//cds/enzyme/phosphorylation
type: Collection
persistent identity: cds enzyme phosphorylation
display id: cds_enzyme_phosphorylation
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
owned by: james
owned by: myers
member: BBa_J212099
member: BBa_K283008
member: BBa_K283020
member: BBa_K094100
member: BBa_K136046
member: pck
member: VanBP
member: tar-envZ
member: BBa_K147000
member: BBa_K819009
member: BBa_K557000
member: lsrK
member: BBa_K118015
member: cheZ
member: BBa_K118016
member: BBa_I752001
member: BBa_K1412829
member: BBa_K932000
member: trz

BBa_K932000
type: ComponentDefinition
persistent identity: BBa K932000
display id: BBa_K932000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: OmpR234 under Tet Repressible Promoter
creator: Sean Kearney
created: 2012-09-15T11:00:00Z
modified: 2015-05-08T01:13:47Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1197_
m user id: 0
owner id: 6891
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part promotes the constitutive expression of the curli cluster (csgD), proteins involved in cell-cell adhesion.
mutable notes: This part regulates curli expression within the cell via transcriptional activation. The construct can be repressed in the presence of the TetR repressor.
mutable provenance: Comes from the E. coli K-12 genome.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0015
component: BBa_R0040
component: BBa_K932001
sequence annotation: BBa_B0015
sequence annotation: BBa_R0040
sequence annotation: BBa_K932001
sequence: https://synbiohub.org/public/igem/BBa_K932000_sequence/1

BBa_B0015
type: Component
persistent identity: component2210137
display id: component2210137
version: 1
top level: BBa_K932000
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_R0040
type: Component
persistent identity: component2210124
display id: component2210124
version: 1
top level: BBa_K932000
access: public
definition: https://synbiohub.org/public/igem/BBa_R0040/1

BBa_K932001
type: Component
persistent identity: component2210130
display id: component2210130
version: 1
top level: BBa_K932000
access: public
definition: https://synbiohub.org/public/igem/BBa_K932001/1

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2210137
display id: annotation2210137
version: 1
top level: BBa_K932000
location: https://synbiohub.org/public/igem/BBa_K932000/annotation2210137/range2210137/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K932000/annotation2210137/range2210137/1
type: Range
persistent identity: range2210137
display id: range2210137
version: 1
direction: direction/reverse
top level: BBa_K932000
start: 789
end: 917
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation2210124
display id: annotation2210124
version: 1
top level: BBa_K932000
location: https://synbiohub.org/public/igem/BBa_K932000/annotation2210124/range2210124/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_K932000/annotation2210124/range2210124/1
type: Range
persistent identity: range2210124
display id: range2210124
version: 1
direction: direction/forward
top level: BBa_K932000
start: 1
end: 54
orientation: inline

BBa_K932001
type: SequenceAnnotation
persistent identity: annotation2210130
display id: annotation2210130
version: 1
top level: BBa_K932000
location: https://synbiohub.org/public/igem/BBa_K932000/annotation2210130/range2210130/1
component: BBa_K932001

https://synbiohub.org/public/igem/BBa_K932000/annotation2210130/range2210130/1
type: Range
persistent identity: range2210130
display id: range2210130
version: 1
direction: direction/reverse
top level: BBa_K932000
start: 61
end: 780
orientation: inline

BBa_K1412829
type: ComponentDefinition
persistent identity: BBa K1412829
display id: BBa_K1412829
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Characterize efficiency of RBS with chemotaxis
creator: Ruihua Zhang
created: 2014-09-01T11:00:00Z
modified: 2015-05-08T01:10:18Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1790_
m user id: 0
owner id: 22460
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: BBa_R0010 BBa_B0033 BBa_K629003 BBa_B0015 Plac-RBS(0.01)-CheZ-TT This part can express CheZ protein which decide the E.coli whether tumble of swim straight.In return, we can charecterize the efficiency of RBS and Promoter by measure the distance E.coli migration. 3H:13-P3-3H BBa_R0010 2L:14-P2-2L BBa_B0033 18G:14-P1-18G BBa_K629003 4F:13-P3-4F BBa_B0015
mutable notes: 1.link RBS and Promoter is too short to link to other gene part.
mutable provenance: BBa_R0010: 13-P3-3H BBa_B0033: 14-P2-2L BBa_K629003: 14-P1-18G BBa_B0015: 13-P3-4F
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_K629003
component: BBa_R0010
component: BBa_B0015
component: BBa_B0032
sequence annotation: BBa_B0015
sequence annotation: BBa_B0032
sequence annotation: BBa_K629003
sequence annotation: BBa_R0010
sequence: https://synbiohub.org/public/igem/BBa_K1412829_sequence/1

BBa_K629003
type: Component
persistent identity: component2393039
display id: component2393039
version: 1
top level: BBa_K1412829
access: public
definition: cheZ

BBa_R0010
type: Component
persistent identity: component2393023
display id: component2393023
version: 1
top level: BBa_K1412829
access: public
definition: https://synbiohub.org/public/igem/BBa_R0010/1

BBa_B0015
type: Component
persistent identity: component2393046
display id: component2393046
version: 1
top level: BBa_K1412829
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_B0032
type: Component
persistent identity: component2393031
display id: component2393031
version: 1
top level: BBa_K1412829
access: public
definition: https://synbiohub.org/public/igem/BBa_B0032/1

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2393046
display id: annotation2393046
version: 1
top level: BBa_K1412829
location: https://synbiohub.org/public/igem/BBa_K1412829/annotation2393046/range2393046/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K1412829/annotation2393046/range2393046/1
type: Range
persistent identity: range2393046
display id: range2393046
version: 1
direction: direction/reverse
top level: BBa_K1412829
start: 881
end: 1009
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation2393031
display id: annotation2393031
version: 1
top level: BBa_K1412829
location: https://synbiohub.org/public/igem/BBa_K1412829/annotation2393031/range2393031/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_K1412829/annotation2393031/range2393031/1
type: Range
persistent identity: range2393031
display id: range2393031
version: 1
direction: direction/forward
top level: BBa_K1412829
start: 209
end: 221
orientation: inline

BBa_K629003
type: SequenceAnnotation
persistent identity: annotation2393039
display id: annotation2393039
version: 1
top level: BBa_K1412829
location: https://synbiohub.org/public/igem/BBa_K1412829/annotation2393039/range2393039/1
component: BBa_K629003

https://synbiohub.org/public/igem/BBa_K1412829/annotation2393039/range2393039/1
type: Range
persistent identity: range2393039
display id: range2393039
version: 1
direction: direction/reverse
top level: BBa_K1412829
start: 228
end: 872
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation2393023
display id: annotation2393023
version: 1
top level: BBa_K1412829
location: https://synbiohub.org/public/igem/BBa_K1412829/annotation2393023/range2393023/1
component: BBa_R0010

https://synbiohub.org/public/igem/BBa_K1412829/annotation2393023/range2393023/1
type: Range
persistent identity: range2393023
display id: range2393023
version: 1
direction: direction/forward
top level: BBa_K1412829
start: 1
end: 200
orientation: inline

BBa_K557000
type: ComponentDefinition
persistent identity: BBa K557000
display id: BBa_K557000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CheZ: phosphatase which dephosphorylates CheY-P and thus plays an important role in E.Coli chemotaxi
creator: Yinghong Lan
created: 2011-10-01T11:00:00Z
modified: 2015-05-08T01:12:41Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _725_
m user id: 0
owner id: 8574
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part encodes CheZ, which is a phosphatase which dephosphorylates CheY-P and causes the flagellum to rotate counterclockwise. E.coli lacking the cheZ gene cannot dephosphorylate CheY-P, tumble incessantly, and are thus nonmotile while inducing the expression of CheZ can restore motility in a CheZ knockout strain by reactivating wild-type chemotaxis.
mutable notes: We get it from the genome of Top10 by PCR.
mutable provenance: The genome of Top10, a strain of E.coli
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: start codon
sequence: https://synbiohub.org/public/igem/BBa_K557000_sequence/1

start codon
type: SequenceAnnotation
persistent identity: annotation2149325
display id: annotation2149325
version: 1
top level: BBa_K557000
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K557000/annotation2149325/range2149325/1

https://synbiohub.org/public/igem/BBa_K557000/annotation2149325/range2149325/1
type: Range
persistent identity: range2149325
display id: range2149325
version: 1
direction: direction/forward
top level: BBa_K557000
start: 1
end: 3
orientation: inline

BBa_K283008
type: ComponentDefinition
persistent identity: BBa K283008
display id: BBa_K283008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: chez_Histag
creator: W Ling , Nelson Chu, Shi Lei
created: 2009-10-12T11:00:00Z
modified: 2015-05-08T01:11:47Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _383_
m user id: 0
owner id: 5494
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This part consists of a chez coding region followed by a 6-Histidine tail
mutable notes: BBa_K283008
mutable provenance: self-designed
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: BBa_K094100
sequence annotation: cheZ
sequence annotation: 6-His tag
sequence: https://synbiohub.org/public/igem/BBa_K283008_sequence/1

BBa_K094100
type: SequenceAnnotation
persistent identity: annotation2035290
display id: annotation2035290
version: 1
top level: BBa_K283008
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K283008/annotation2035290/range2035290/1

https://synbiohub.org/public/igem/BBa_K283008/annotation2035290/range2035290/1
type: Range
persistent identity: range2035290
display id: range2035290
version: 1
direction: direction/reverse
top level: BBa_K283008
start: 1
end: 695
orientation: inline

cheZ
type: SequenceAnnotation
persistent identity: annotation2035289
display id: annotation2035289
version: 1
top level: BBa_K283008
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K283008/annotation2035289/range2035289/1

https://synbiohub.org/public/igem/BBa_K283008/annotation2035289/range2035289/1
type: Range
persistent identity: range2035289
display id: range2035289
version: 1
direction: direction/forward
top level: BBa_K283008
start: 1
end: 645
orientation: inline

6-His tag
type: SequenceAnnotation
persistent identity: annotation2035366
display id: annotation2035366
version: 1
top level: BBa_K283008
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K283008/annotation2035366/range2035366/1

https://synbiohub.org/public/igem/BBa_K283008/annotation2035366/range2035366/1
type: Range
persistent identity: range2035366
display id: range2035366
version: 1
direction: direction/reverse
top level: BBa_K283008
start: 696
end: 713
orientation: inline

trz
type: ComponentDefinition
persistent identity: BBa J58104
display id: BBa_J58104
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Fusion protein Trg-EnvZ for signal transduction
creator: Valencia iGEM 2006
created: 2006-10-25T11:00:00Z
modified: 2015-08-31T03:14:48Z
discontinued: false
dominant: false
experience: experience/None
group u list: _83_
m user id: 0
owner id: 1100
owning group id: 83
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: We created a hybrid gene coding for a protein in which Trg (chemoreceptor) contributed its periplasmic and transmembrane domains as well as a short cytoplasmic segment and EnvZ (osmosensor) contributed its cytoplasmic kinase/phosphatase domain.
mutable notes: none
mutable provenance: none
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_J58104_sequence/1

BBa_K094100
type: ComponentDefinition
persistent identity: BBa K094100
display id: BBa_K094100
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: cheZ gene
creator: Liu Chenli
created: 2008-10-12T11:00:00Z
modified: 2015-05-08T01:08:40Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _255_
m user id: 0
owner id: 2589
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part contains the cheZ operon coding region without an rbs nor a terminator. cheZ protein is responsible for the dephosphorylation of cheY protein in bacteria flagella movement.
mutable notes: This part is without promoter, rbs or terminator.
mutable provenance: The origin of the cheZ gene comes from E. Coli strain MG1655 chromosome.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: cheZ
sequence: https://synbiohub.org/public/igem/BBa_K094100_sequence/1

cheZ
type: SequenceAnnotation
persistent identity: annotation1980478
display id: annotation1980478
version: 1
top level: BBa_K094100
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K094100/annotation1980478/range1980478/1

https://synbiohub.org/public/igem/BBa_K094100/annotation1980478/range1980478/1
type: Range
persistent identity: range1980478
display id: range1980478
version: 1
direction: direction/forward
top level: BBa_K094100
start: 1
end: 645
orientation: inline

BBa_K136046
type: ComponentDefinition
persistent identity: BBa K136046
display id: BBa_K136046
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: envZ*
creator: alexandra bouaziz
created: 2008-10-24T11:00:00Z
modified: 2015-05-08T01:10:03Z
discontinued: false
dominant: false
group u list: _211_
m user id: 0
owner id: 3215
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: EnvZ* is a transmenbrane kinase that phosphorylates ompR the natural inhibitor of pFlhDC independently of any outer signal.
mutable notes: PCR and ligation in pCR4Blunt-TOPO
mutable provenance: bacterial genome of mutated (I281S) strain for EnvZ.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: I281S T->G
sequence annotation: TAA
sequence annotation: ATG
sequence: https://synbiohub.org/public/igem/BBa_K136046_sequence/1

I281S T->G
type: SequenceAnnotation
persistent identity: annotation1994273
display id: annotation1994273
version: 1
top level: BBa_K136046
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_K136046/annotation1994273/range1994273/1

https://synbiohub.org/public/igem/BBa_K136046/annotation1994273/range1994273/1
type: Range
persistent identity: range1994273
display id: range1994273
version: 1
direction: direction/forward
top level: BBa_K136046
start: 842
end: 842
orientation: inline

TAA
type: SequenceAnnotation
persistent identity: annotation1994303
display id: annotation1994303
version: 1
top level: BBa_K136046
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K136046/annotation1994303/range1994303/1

https://synbiohub.org/public/igem/BBa_K136046/annotation1994303/range1994303/1
type: Range
persistent identity: range1994303
display id: range1994303
version: 1
direction: direction/forward
top level: BBa_K136046
start: 1351
end: 1353
orientation: inline

ATG
type: SequenceAnnotation
persistent identity: annotation1994304
display id: annotation1994304
version: 1
top level: BBa_K136046
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K136046/annotation1994304/range1994304/1

https://synbiohub.org/public/igem/BBa_K136046/annotation1994304/range1994304/1
type: Range
persistent identity: range1994304
display id: range1994304
version: 1
direction: direction/forward
top level: BBa_K136046
start: 1
end: 3
orientation: inline

pck
type: ComponentDefinition
persistent identity: BBa K2135000
display id: BBa_K2135000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pck
creator: Harley Greene
created: 2016-08-25T11:00:00Z
modified: 2016-10-14T11:28:26Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2604_
m user id: 29300
owner id: 29300
owning group id: 9
status: status/Planning
bookmark: false
mutable description: pck codes for Phosphoenolpyruvate carboxykinase (Pck), a kinase involved in gluconeogenesis in E. coli
mutable notes: No codons were modified to make this part
mutable provenance: pck gene came from E coli strain MG1655
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: pck
sequence annotation: Native RBS
sequence: https://synbiohub.org/public/igem/BBa_K2135000_sequence/1

pck
type: SequenceAnnotation
persistent identity: annotation2481661
display id: annotation2481661
version: 1
top level: pck
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K2135000/annotation2481661/range2481661/1

https://synbiohub.org/public/igem/BBa_K2135000/annotation2481661/range2481661/1
type: Range
persistent identity: range2481661
display id: range2481661
version: 1
direction: direction/forward
top level: pck
start: 66
end: 1686
orientation: inline

Native RBS
type: SequenceAnnotation
persistent identity: annotation2481660
display id: annotation2481660
version: 1
top level: pck
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K2135000/annotation2481660/range2481660/1

https://synbiohub.org/public/igem/BBa_K2135000/annotation2481660/range2481660/1
type: Range
persistent identity: range2481660
display id: range2481660
version: 1
direction: direction/forward
top level: pck
start: 1
end: 65
orientation: inline

BBa_K283020
type: ComponentDefinition
persistent identity: BBa K283020
display id: BBa_K283020
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: FF_FF ansB promoter-rbs-cheZ_histag-terminators
creator: W Ling , Nelson Chu, Shi Lei
created: 2009-10-12T11:00:00Z
modified: 2015-05-08T01:11:47Z
discontinued: false
dominant: false
experience: experience/None
group u list: _383_
m user id: 0
owner id: 5494
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part consist of a chez protein linked to a double terminator. The cheZ protein is responsible for the dephosphorylation of cheY protein in bacteria flagella movement. The chez coding region has a 6-Histidine tail and do not contain any rbs.
mutable notes: BBa_K283020
mutable provenance: The chez-histag coding region is self-designed The double terminator is from the registry
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_B0034
component: BBa_B0010
component: BBa_K283031
component: BBa_K283008
component: BBa_B0012
sequence annotation: BBa_B0012
sequence annotation: BBa_K283031
sequence annotation: BBa_K283008
sequence annotation: BBa_B0010
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K283020_sequence/1

BBa_B0034
type: Component
persistent identity: component2056620
display id: component2056620
version: 1
top level: BBa_K283020
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0010
type: Component
persistent identity: component2056625
display id: component2056625
version: 1
top level: BBa_K283020
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_K283031
type: Component
persistent identity: component2056618
display id: component2056618
version: 1
top level: BBa_K283020
access: public
definition: https://synbiohub.org/public/igem/BBa_K283031/1

BBa_K283008
type: Component
persistent identity: component2056624
display id: component2056624
version: 1
top level: BBa_K283020
access: public
definition: BBa_K283008

BBa_B0012
type: Component
persistent identity: component2056627
display id: component2056627
version: 1
top level: BBa_K283020
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2056627
display id: annotation2056627
version: 1
top level: BBa_K283020
location: https://synbiohub.org/public/igem/BBa_K283020/annotation2056627/range2056627/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K283020/annotation2056627/range2056627/1
type: Range
persistent identity: range2056627
display id: range2056627
version: 1
direction: direction/forward
top level: BBa_K283020
start: 941
end: 981
orientation: inline

BBa_K283031
type: SequenceAnnotation
persistent identity: annotation2056618
display id: annotation2056618
version: 1
top level: BBa_K283020
location: https://synbiohub.org/public/igem/BBa_K283020/annotation2056618/range2056618/1
component: BBa_K283031

https://synbiohub.org/public/igem/BBa_K283020/annotation2056618/range2056618/1
type: Range
persistent identity: range2056618
display id: range2056618
version: 1
direction: direction/reverse
top level: BBa_K283020
start: 1
end: 105
orientation: inline

BBa_K283008
type: SequenceAnnotation
persistent identity: annotation2056624
display id: annotation2056624
version: 1
top level: BBa_K283020
location: https://synbiohub.org/public/igem/BBa_K283020/annotation2056624/range2056624/1
component: BBa_K283008

https://synbiohub.org/public/igem/BBa_K283020/annotation2056624/range2056624/1
type: Range
persistent identity: range2056624
display id: range2056624
version: 1
direction: direction/reverse
top level: BBa_K283020
start: 132
end: 844
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2056625
display id: annotation2056625
version: 1
top level: BBa_K283020
location: https://synbiohub.org/public/igem/BBa_K283020/annotation2056625/range2056625/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K283020/annotation2056625/range2056625/1
type: Range
persistent identity: range2056625
display id: range2056625
version: 1
direction: direction/forward
top level: BBa_K283020
start: 853
end: 932
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2056620
display id: annotation2056620
version: 1
top level: BBa_K283020
location: https://synbiohub.org/public/igem/BBa_K283020/annotation2056620/range2056620/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K283020/annotation2056620/range2056620/1
type: Range
persistent identity: range2056620
display id: range2056620
version: 1
direction: direction/reverse
top level: BBa_K283020
start: 114
end: 125
orientation: inline

BBa_K819009
type: ComponentDefinition
persistent identity: BBa K819009
display id: BBa_K819009
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Weak cheZ generator
creator: Xing Wenmin
created: 2012-09-02T11:00:00Z
modified: 2015-05-08T01:13:29Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1077_
m user id: 0
owner id: 13785
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: cheZ ligated to a weak promoter. This part express CheZ protein weakly and will induce weak mobility in delta cheZ E.coli strain.
mutable notes: There is a point mutation at position 56 of CheZ coding sequence with have little effect to its function and the bases corresponding to stop codon is changed to bases coding a more frequently used stop codon.
mutable provenance: The promoter used here is from Partsregistry. The CheZ gene is from E.coli strain MG1655 by colony PCR and aligned with CheZ sequence at EcoGene database.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: CheZ
sequence annotation: B0034
sequence annotation: BBa_J23112
sequence: https://synbiohub.org/public/igem/BBa_K819009_sequence/1

CheZ
type: SequenceAnnotation
persistent identity: annotation2197345
display id: annotation2197345
version: 1
top level: BBa_K819009
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K819009/annotation2197345/range2197345/1

https://synbiohub.org/public/igem/BBa_K819009/annotation2197345/range2197345/1
type: Range
persistent identity: range2197345
display id: range2197345
version: 1
direction: direction/forward
top level: BBa_K819009
start: 62
end: 703
orientation: inline

B0034
type: SequenceAnnotation
persistent identity: annotation2197343
display id: annotation2197343
version: 1
top level: BBa_K819009
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K819009/annotation2197343/range2197343/1

https://synbiohub.org/public/igem/BBa_K819009/annotation2197343/range2197343/1
type: Range
persistent identity: range2197343
display id: range2197343
version: 1
direction: direction/forward
top level: BBa_K819009
start: 44
end: 55
orientation: inline

BBa_J23112
type: SequenceAnnotation
persistent identity: annotation2197342
display id: annotation2197342
version: 1
top level: BBa_K819009
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K819009/annotation2197342/range2197342/1

https://synbiohub.org/public/igem/BBa_K819009/annotation2197342/range2197342/1
type: Range
persistent identity: range2197342
display id: range2197342
version: 1
direction: direction/forward
top level: BBa_K819009
start: 1
end: 35
orientation: inline

BBa_J212099
type: ComponentDefinition
persistent identity: BBa J212099
display id: BBa_J212099
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: test
creator: Shuangxi Ren
created: 2012-02-03T12:00:00Z
modified: 2015-08-31T04:08:37Z
discontinued: true
dominant: false
experience: experience/None
group u list: _921_
m user id: 0
owner id: 11311
owning group id: 9
sample status: Discontinued
status: status/Deleted
bookmark: false
mutable description: this is a test.
mutable notes: rfc10
mutable provenance: home-made
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000110
role: partType/Temporary
sequence annotation: rbs
sequence: https://synbiohub.org/public/igem/BBa_J212099_sequence/1

rbs
type: SequenceAnnotation
persistent identity: annotation2170086
display id: annotation2170086
version: 1
top level: BBa_J212099
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_J212099/annotation2170086/range2170086/1

https://synbiohub.org/public/igem/BBa_J212099/annotation2170086/range2170086/1
type: Range
persistent identity: range2170086
display id: range2170086
version: 1
direction: direction/forward
top level: BBa_J212099
start: 1
end: 5
orientation: inline

cheZ
type: ComponentDefinition
persistent identity: BBa K629003
display id: BBa_K629003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: cheZ, chemotaxis regulator, protein phosphatase for CheY
creator: Zilong WANG, Yi ZHENG
created: 2011-10-02T11:00:00Z
modified: 2015-05-08T01:12:56Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _801_
m user id: 0
owner id: 9022
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Polar localization is dependent on CheA-short. CheZ is a cytosolic phosphatase which functions in the chemotaxis signal transduction complex. Plays an important role in bacterial chemotaxis signal transduction pathway by accelerating the dephosphorylation of phosphorylated CheY (CheY-P).
mutable notes: how to control the proper expression of cheZ
mutable provenance: genome of E. Coli BL21 plys strain
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: start
sequence annotation: fwd primer
sequence annotation: rev primer
sequence annotation: TGA to TAA
sequence annotation: terminator
sequence annotation: CheZ
sequence: https://synbiohub.org/public/igem/BBa_K629003_sequence/1

start
type: SequenceAnnotation
persistent identity: annotation2154422
display id: annotation2154422
version: 1
top level: cheZ
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K629003/annotation2154422/range2154422/1

https://synbiohub.org/public/igem/BBa_K629003/annotation2154422/range2154422/1
type: Range
persistent identity: range2154422
display id: range2154422
version: 1
direction: direction/forward
top level: cheZ
start: 1
end: 3
orientation: inline

fwd primer
type: SequenceAnnotation
persistent identity: annotation2154421
display id: annotation2154421
version: 1
top level: cheZ
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K629003/annotation2154421/range2154421/1

https://synbiohub.org/public/igem/BBa_K629003/annotation2154421/range2154421/1
type: Range
persistent identity: range2154421
display id: range2154421
version: 1
direction: direction/forward
top level: cheZ
start: 1
end: 20
orientation: inline

rev primer
type: SequenceAnnotation
persistent identity: annotation2154423
display id: annotation2154423
version: 1
top level: cheZ
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K629003/annotation2154423/range2154423/1

https://synbiohub.org/public/igem/BBa_K629003/annotation2154423/range2154423/1
type: Range
persistent identity: range2154423
display id: range2154423
version: 1
direction: direction/forward
top level: cheZ
start: 624
end: 645
orientation: inline

TGA to TAA
type: SequenceAnnotation
persistent identity: annotation2155668
display id: annotation2155668
version: 1
top level: cheZ
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K629003/annotation2155668/range2155668/1

https://synbiohub.org/public/igem/BBa_K629003/annotation2155668/range2155668/1
type: Range
persistent identity: range2155668
display id: range2155668
version: 1
direction: direction/forward
top level: cheZ
start: 643
end: 645
orientation: inline

terminator
type: SequenceAnnotation
persistent identity: annotation2154424
display id: annotation2154424
version: 1
top level: cheZ
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K629003/annotation2154424/range2154424/1

https://synbiohub.org/public/igem/BBa_K629003/annotation2154424/range2154424/1
type: Range
persistent identity: range2154424
display id: range2154424
version: 1
direction: direction/forward
top level: cheZ
start: 643
end: 645
orientation: inline

CheZ
type: SequenceAnnotation
persistent identity: annotation2154425
display id: annotation2154425
version: 1
top level: cheZ
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K629003/annotation2154425/range2154425/1

https://synbiohub.org/public/igem/BBa_K629003/annotation2154425/range2154425/1
type: Range
persistent identity: range2154425
display id: range2154425
version: 1
direction: direction/forward
top level: cheZ
start: 1
end: 645
orientation: inline

BBa_I752001
type: ComponentDefinition
persistent identity: BBa I752001
display id: BBa_I752001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CheZ coding sequence (Chemotaxis protein)
creator: David Franz
created: 2007-08-26T11:00:00Z
modified: 2016-10-08T03:28:13Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _129_
m user id: 25212
owner id: 1631
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: CheZ is one of the 6 Che proteins used in chemeotaxis. CheZ is a phosphatase which dephosphorylates CheY which in turn then allows the cell to run and tumble, wheres if CheZ is absence, CheY is phosphorylated, and the cell tumbles exclusively. (Topp and Gallivan J. Am. Chem. Soc. 129, p6865-6873)
mutable notes: blah
mutable provenance: blah
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: CheZ
sequence: https://synbiohub.org/public/igem/BBa_I752001_sequence/1

CheZ
type: SequenceAnnotation
persistent identity: annotation1944568
display id: annotation1944568
version: 1
top level: BBa_I752001
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_I752001/annotation1944568/range1944568/1

https://synbiohub.org/public/igem/BBa_I752001/annotation1944568/range1944568/1
type: Range
persistent identity: range1944568
display id: range1944568
version: 1
direction: direction/forward
top level: BBa_I752001
start: 1
end: 639
orientation: inline

VanBP
type: ComponentDefinition
persistent identity: BBa J58105
display id: BBa_J58105
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Synthetic periplasmic binding protein that docks a vanillin molecule
creator: Valencia iGEM 2006
created: 2006-10-25T11:00:00Z
modified: 2015-08-31T03:14:48Z
discontinued: false
dominant: false
experience: experience/None
group u list: _83_
m user id: 0
owner id: 1100
owning group id: 83
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: none
mutable notes: none
mutable provenance: none
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_J58105_sequence/1

BBa_K147000
type: ComponentDefinition
persistent identity: BBa K147000
display id: BBa_K147000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: cheZ
creator: Roxanne Shank
created: 2008-10-27T12:00:00Z
modified: 2015-05-08T01:10:38Z
discontinued: false
dominant: false
group u list: _242_
m user id: 0
owner id: 3171
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: The cheZ motility protein is a protein kinase which dephosphorylates cheY. cheY regulates the clockwise and counterclockwise rotation of the motor flagella. If cheY is phosphorylated the motor flagella are not in sync and the bacterium tumbles. If cheY is not phosphorylated the motor flagella are in sync and the bacterium runs (often towards a chemoattractant)
mutable notes: Nothing of note
mutable provenance: Amplified from pTopp plasmid.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K147000_sequence/1

lsrK
type: ComponentDefinition
persistent identity: BBa K091002
display id: BBa_K091002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LsrK gene
creator: Robert Cool
created: 2008-06-18T11:00:00Z
modified: 2015-05-08T01:08:37Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _191_
m user id: 0
owner id: 3080
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This part codes for the protein LsrK which phosphorylates AI-2 which acts to promote the lsrA promoter.
mutable notes: no special considerations
mutable provenance: This sequence is found in the E. coli strain MG1655.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K091002_sequence/1

BBa_K118016
type: ComponentDefinition
persistent identity: BBa K118016
display id: BBa_K118016
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: glgC16 (glgC with G336D substitution)
creator: Andrew Hall
created: 2008-10-05T11:00:00Z
modified: 2015-05-08T01:09:37Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _192_
m user id: 0
owner id: 3282
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: This is the coding sequence of ''glgC'' (ADP-glucose pyrophosphorylase) from ''Escherichia coli'' JM109 with the substitution G336D. This mutation is known to cause increased activity of ADP-glucose pyrophosphorylase in the absence of the activator fructose 1,6-bisphosphate (FBP), high affinity for FBP and substrates lower affinity for the inhibitor AMP. (Leung ''et al'', 1986; Meyer ''et al.'', 1988)
mutable notes: The mutation G336D was achieved by the substitution of A for G at position 1076 (codon GGC->GAC) by MABEL.
mutable provenance: ''Escherichia coli'' JM109 genomic DNA
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: G336D
sequence annotation: EcoRI site removed
sequence: https://synbiohub.org/public/igem/BBa_K118016_sequence/1

G336D
type: SequenceAnnotation
persistent identity: annotation1978557
display id: annotation1978557
version: 1
top level: BBa_K118016
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K118016/annotation1978557/range1978557/1

https://synbiohub.org/public/igem/BBa_K118016/annotation1978557/range1978557/1
type: Range
persistent identity: range1978557
display id: range1978557
version: 1
direction: direction/forward
top level: BBa_K118016
start: 1006
end: 1008
orientation: inline

EcoRI site removed
type: SequenceAnnotation
persistent identity: annotation1978559
display id: annotation1978559
version: 1
top level: BBa_K118016
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_K118016/annotation1978559/range1978559/1

https://synbiohub.org/public/igem/BBa_K118016/annotation1978559/range1978559/1
type: Range
persistent identity: range1978559
display id: range1978559
version: 1
direction: direction/forward
top level: BBa_K118016
start: 1068
end: 1073
orientation: inline

EcoRI site removed
type: SequenceAnnotation
persistent identity: annotation1978558
display id: annotation1978558
version: 1
top level: BBa_K118016
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_K118016/annotation1978558/range1978558/1

https://synbiohub.org/public/igem/BBa_K118016/annotation1978558/range1978558/1
type: Range
persistent identity: range1978558
display id: range1978558
version: 1
direction: direction/forward
top level: BBa_K118016
start: 571
end: 576
orientation: inline

BBa_K118015
type: ComponentDefinition
persistent identity: BBa K118015
display id: BBa_K118015
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: glgC coding sequence encoding ADP-glucose pyrophosphorylase
creator: Andrew Hall
created: 2008-10-05T11:00:00Z
modified: 2015-05-08T01:09:37Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _192_
m user id: 0
owner id: 3282
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: This is the coding sequence of ''glgC'' from ''Escherichia coli'' JM109. It encodes ADP-glucose pyrophosphorylase, which catalyses Glucose 1-phosphate + ATP -> ADP-glucose + PPi, a step in the production of glycogen from glucose.
mutable notes: The genomic DNA sequence contains to EcoRI sites, both of which have been mutated out using the mutagenesis by blunt-ended ligation (MABEL) technique
mutable provenance: ''Escherichia coli'' JM109 genomic DNA.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: EcoRI site removed
sequence: https://synbiohub.org/public/igem/BBa_K118015_sequence/1

EcoRI site removed
type: SequenceAnnotation
persistent identity: annotation1978556
display id: annotation1978556
version: 1
top level: BBa_K118015
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_K118015/annotation1978556/range1978556/1

https://synbiohub.org/public/igem/BBa_K118015/annotation1978556/range1978556/1
type: Range
persistent identity: range1978556
display id: range1978556
version: 1
direction: direction/forward
top level: BBa_K118015
start: 1068
end: 1073
orientation: inline

EcoRI site removed
type: SequenceAnnotation
persistent identity: annotation1978555
display id: annotation1978555
version: 1
top level: BBa_K118015
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_K118015/annotation1978555/range1978555/1

https://synbiohub.org/public/igem/BBa_K118015/annotation1978555/range1978555/1
type: Range
persistent identity: range1978555
display id: range1978555
version: 1
direction: direction/forward
top level: BBa_K118015
start: 571
end: 576
orientation: inline

tar-envZ
type: ComponentDefinition
persistent identity: BBa C0082
display id: BBa_C0082
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Receptor, tar-envZ
creator: Stephen Lee, Roshan Kumar, Joe Levine, Ziyan Chu (Polkadorks, IAP 2004)
created: 2004-01-27T12:00:00Z
modified: 2015-08-31T04:07:24Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Coding sequence for Tar-EnvZ fusion protein. Tar is the bacterial chemotaxis receptor for aspartate. EnvZ is an osmosensor kinase, which, through phosphorylation of OmpR, regulates transcription of porin genes. This fusion protein combines the extracellular and transmembrane domains of Tar with the intracellular domain of EnvZ. The fusion converts chemical binding of aspartate to production of transcription factors.
mutable notes: A silent point mutation was made at base 423 (A -> C) to eliminate an XbaI restriction enzyme site. The mutation was designed to match codons with similar frequencies of usage in E. coli.
mutable provenance: NC_000913 E. coli K12
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Envz (back)
sequence annotation: Tar (front)
sequence annotation: A
sequence annotation: Help:Barcodes
sequence annotation: LVA
sequence: https://synbiohub.org/public/igem/BBa_C0082_sequence/1

Envz (back)
type: SequenceAnnotation
persistent identity: annotation308360
display id: annotation308360
version: 1
top level: tar-envZ
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_C0082/annotation308360/range308360/1

https://synbiohub.org/public/igem/BBa_C0082/annotation308360/range308360/1
type: Range
persistent identity: range308360
display id: range308360
version: 1
direction: direction/forward
top level: tar-envZ
start: 772
end: 1452
orientation: inline

Tar (front)
type: SequenceAnnotation
persistent identity: annotation308352
display id: annotation308352
version: 1
top level: tar-envZ
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_C0082/annotation308352/range308352/1

https://synbiohub.org/public/igem/BBa_C0082/annotation308352/range308352/1
type: Range
persistent identity: range308352
display id: range308352
version: 1
direction: direction/forward
top level: tar-envZ
start: 1
end: 765
orientation: inline

A
type: SequenceAnnotation
persistent identity: annotation300943
display id: annotation300943
version: 1
top level: tar-envZ
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_C0082/annotation300943/range300943/1

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LVA
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