//cds/lysis
type: Collection
persistent identity: cds lysis
display id: cds_lysis
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
owned by: james
owned by: myers
member: BBa_K608352
member: gpS
member: BBa_K772101
member: BBa_K108001
member: BBa_K990005
member: LysA
member: BBa_K117000
member: BBa_K124017
member: BBa_K284022
member: BBa_K358004
member: Lysis

BBa_K990005
type: ComponentDefinition
persistent identity: BBa K990005
display id: BBa_K990005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LasR Device + T4 Lysis Device
creator: Fatma Betul CEVIK
created: 2013-06-20T11:00:00Z
modified: 2015-05-08T01:13:51Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1287_
m user id: 0
owner id: 11713
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: Receiver cell, T4 lysis device is under pLas.
mutable notes: We got BBa_K772004 from 2012 Fatih Medical team.
mutable provenance: LasR Device:K772004 Enterobacteria phage T4 Lysis Device - no promoter: K112808
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K772004
component: BBa_K112808
sequence annotation: BBa_K112808
sequence annotation: BBa_K772004
sequence: https://synbiohub.org/public/igem/BBa_K990005_sequence/1

BBa_K772004
type: Component
persistent identity: component2329973
display id: component2329973
version: 1
top level: BBa_K990005
access: public
definition: https://synbiohub.org/public/igem/BBa_K772004/1

BBa_K112808
type: Component
persistent identity: component2329989
display id: component2329989
version: 1
top level: BBa_K990005
access: public
definition: https://synbiohub.org/public/igem/BBa_K112808/1

BBa_K112808
type: SequenceAnnotation
persistent identity: annotation2329989
display id: annotation2329989
version: 1
top level: BBa_K990005
location: https://synbiohub.org/public/igem/BBa_K990005/annotation2329989/range2329989/1
component: BBa_K112808

https://synbiohub.org/public/igem/BBa_K990005/annotation2329989/range2329989/1
type: Range
persistent identity: range2329989
display id: range2329989
version: 1
direction: direction/reverse
top level: BBa_K990005
start: 1131
end: 2915
orientation: inline

BBa_K772004
type: SequenceAnnotation
persistent identity: annotation2329973
display id: annotation2329973
version: 1
top level: BBa_K990005
location: https://synbiohub.org/public/igem/BBa_K990005/annotation2329973/range2329973/1
component: BBa_K772004

https://synbiohub.org/public/igem/BBa_K990005/annotation2329973/range2329973/1
type: Range
persistent identity: range2329973
display id: range2329973
version: 1
direction: direction/reverse
top level: BBa_K990005
start: 1
end: 1122
orientation: inline

BBa_K772101
type: ComponentDefinition
persistent identity: BBa K772101
display id: BBa_K772101
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Self-Destruction Kit: Endolysin + Holin
creator: Mustafa Elitok
created: 2012-09-25T11:00:00Z
modified: 2015-05-08T01:13:15Z
discontinued: false
dominant: false
group u list: _1024_
m user id: 0
owner id: 9573
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Endolysin is a lysozyme which cleaves the glycosidic bond between the C-1 of N-acetylmuramic acid (NAM) and the C-4 of N-acetylglucosamine (NAG) in the bacterial peptidoglycan (PG). This leads to the complete lysis of the cell. Holin is a protein that assembles together to form pores on inner membrane of bacteria allowing lysozyme to reach periplasm and degrade peptidoglycan layer. It is sourced from T4 bacteriophage. This composite part can be used to induce self-destruction with a regulatory device in the upstream region.
mutable notes: There is no RBS in the upstream region, so during the assemble procedure, this must be taken care of.
mutable provenance: Gene sequences of endolysin and holin are gathered from the Registry.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K112806
component: BBa_K124014
sequence annotation: BBa_K124014
sequence annotation: BBa_K112806
sequence: https://synbiohub.org/public/igem/BBa_K772101_sequence/1

BBa_K112806
type: Component
persistent identity: component2202511
display id: component2202511
version: 1
top level: BBa_K772101
access: public
definition: https://synbiohub.org/public/igem/BBa_K112806/1

BBa_K124014
type: Component
persistent identity: component2202512
display id: component2202512
version: 1
top level: BBa_K772101
access: public
definition: https://synbiohub.org/public/igem/BBa_K124014/1

BBa_K124014
type: SequenceAnnotation
persistent identity: annotation2202512
display id: annotation2202512
version: 1
top level: BBa_K772101
location: https://synbiohub.org/public/igem/BBa_K772101/annotation2202512/range2202512/1
component: BBa_K124014

https://synbiohub.org/public/igem/BBa_K772101/annotation2202512/range2202512/1
type: Range
persistent identity: range2202512
display id: range2202512
version: 1
direction: direction/reverse
top level: BBa_K772101
start: 523
end: 839
orientation: inline

BBa_K112806
type: SequenceAnnotation
persistent identity: annotation2202511
display id: annotation2202511
version: 1
top level: BBa_K772101
location: https://synbiohub.org/public/igem/BBa_K772101/annotation2202511/range2202511/1
component: BBa_K112806

https://synbiohub.org/public/igem/BBa_K772101/annotation2202511/range2202511/1
type: Range
persistent identity: range2202511
display id: range2202511
version: 1
direction: direction/reverse
top level: BBa_K772101
start: 1
end: 514
orientation: inline

BBa_K284022
type: ComponentDefinition
persistent identity: BBa K284022
display id: BBa_K284022
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: T4 endolysin under the control of T7 promotor
creator: Ane Fernanda Beraldi Zeidler, Luige A. Llerena Calder??n and Marcos Henrique de Moraes
created: 2009-10-13T11:00:00Z
modified: 2015-05-08T01:11:48Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _386_
m user id: 0
owner id: 4973
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This construction is a device composed of: BBa_I746911 - BBa_K112806 - Bba_B0014. This device contain the T4 endolysin, a lysozyme from enterobacteria phage, that degrades peptidoglycan layer, under the control of the T7 promoter.
mutable notes: For more accurate tests about the endolysin effectiveness, this part has to be under the control of an inducible promoter, like the T7 promoter.
mutable provenance: This device is a junction of previously designed parts: BBa_I746911 - Designed by Stefan Milde Group: iGEM07_Cambridge (2008-09-30) BBa_K112806 - Designed by Jin Huh Group: iGEM08_UC_Berkeley (2008-10-31) Bba_B0014 - Designed by Reshma Shetty Group: Registry (2003-07-16)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_I719005
component: BBa_B0011
component: BBa_B0034
component: BBa_K112806
component: BBa_B0012
sequence annotation: BBa_B0011
sequence annotation: BBa_B0034
sequence annotation: BBa_K112806
sequence annotation: BBa_I719005
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_K284022_sequence/1

BBa_I719005
type: Component
persistent identity: component2058982
display id: component2058982
version: 1
top level: BBa_K284022
access: public
definition: https://synbiohub.org/public/igem/BBa_I719005/1

BBa_B0011
type: Component
persistent identity: component2058991
display id: component2058991
version: 1
top level: BBa_K284022
access: public
definition: https://synbiohub.org/public/igem/BBa_B0011/1

BBa_B0034
type: Component
persistent identity: component2058984
display id: component2058984
version: 1
top level: BBa_K284022
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K112806
type: Component
persistent identity: component2058986
display id: component2058986
version: 1
top level: BBa_K284022
access: public
definition: https://synbiohub.org/public/igem/BBa_K112806/1

BBa_B0012
type: Component
persistent identity: component2058987
display id: component2058987
version: 1
top level: BBa_K284022
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0011
type: SequenceAnnotation
persistent identity: annotation2058991
display id: annotation2058991
version: 1
top level: BBa_K284022
location: https://synbiohub.org/public/igem/BBa_K284022/annotation2058991/range2058991/1
component: BBa_B0011

https://synbiohub.org/public/igem/BBa_K284022/annotation2058991/range2058991/1
type: Range
persistent identity: range2058991
display id: range2058991
version: 1
direction: direction/forward
top level: BBa_K284022
start: 623
end: 668
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2058984
display id: annotation2058984
version: 1
top level: BBa_K284022
location: https://synbiohub.org/public/igem/BBa_K284022/annotation2058984/range2058984/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K284022/annotation2058984/range2058984/1
type: Range
persistent identity: range2058984
display id: range2058984
version: 1
direction: direction/reverse
top level: BBa_K284022
start: 32
end: 43
orientation: inline

BBa_K112806
type: SequenceAnnotation
persistent identity: annotation2058986
display id: annotation2058986
version: 1
top level: BBa_K284022
location: https://synbiohub.org/public/igem/BBa_K284022/annotation2058986/range2058986/1
component: BBa_K112806

https://synbiohub.org/public/igem/BBa_K284022/annotation2058986/range2058986/1
type: Range
persistent identity: range2058986
display id: range2058986
version: 1
direction: direction/reverse
top level: BBa_K284022
start: 52
end: 565
orientation: inline

BBa_I719005
type: SequenceAnnotation
persistent identity: annotation2058982
display id: annotation2058982
version: 1
top level: BBa_K284022
location: https://synbiohub.org/public/igem/BBa_K284022/annotation2058982/range2058982/1
component: BBa_I719005

https://synbiohub.org/public/igem/BBa_K284022/annotation2058982/range2058982/1
type: Range
persistent identity: range2058982
display id: range2058982
version: 1
direction: direction/reverse
top level: BBa_K284022
start: 1
end: 23
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2058987
display id: annotation2058987
version: 1
top level: BBa_K284022
location: https://synbiohub.org/public/igem/BBa_K284022/annotation2058987/range2058987/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K284022/annotation2058987/range2058987/1
type: Range
persistent identity: range2058987
display id: range2058987
version: 1
direction: direction/forward
top level: BBa_K284022
start: 574
end: 614
orientation: inline

Lysis
type: ComponentDefinition
persistent identity: BBa K836009
display id: BBa_K836009
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Lysis device for R. opacus
creator: Ricardo Alvarado
created: 2012-09-19T11:00:00Z
modified: 2015-05-08T01:13:33Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1095_
m user id: 0
owner id: 11819
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Lysis construct based on the one presented by Berkeley's 2008 team BBa_K112022 (http://partsregistry.org/wiki/index.php?title=Part:BBa_K112022. It is based on the inducible expression of a lysozyme and lambda phage's holin in order to liberate internal products of the bacterial cell in an efficient way. The induction is achieved by the addition of nitrile or a similar agent since both genes are regulated by nitA promoter.
mutable notes: Constitutive expression of nitR and a low constitutive expression of an antiholin are required to avoid undesired lysis.
mutable provenance: The majority of the parts where made by backtranslating a protein. Others are existing biobricks while some are promoters obtained from databases as NCBI. Check each individual part to know its origin.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_K836004
component: BBa_K836006
component: BBa_K836005
component: BBa_B1006
component: BBa_K143012
component: BBa_K836008
component: BBa_K836007
sequence annotation: BBa_K836008
sequence annotation: BBa_K836007
sequence annotation: BBa_K836005
sequence annotation: BBa_B1006
sequence annotation: BBa_K836004
sequence annotation: BBa_K836006
sequence annotation: BBa_K143012
sequence: https://synbiohub.org/public/igem/BBa_K836009_sequence/1

BBa_K836004
type: Component
persistent identity: component2187623
display id: component2187623
version: 1
top level: Lysis
access: public
definition: gpS

BBa_K836006
type: Component
persistent identity: component2187606
display id: component2187606
version: 1
top level: Lysis
access: public
definition: gpS

BBa_K836005
type: Component
persistent identity: component2187602
display id: component2187602
version: 1
top level: Lysis
access: public
definition: https://synbiohub.org/public/igem/BBa_K836005/1

BBa_B1006
type: Component
persistent identity: component2187616
display id: component2187616
version: 1
top level: Lysis
access: public
definition: https://synbiohub.org/public/igem/BBa_B1006/1

BBa_K143012
type: Component
persistent identity: component2187631
display id: component2187631
version: 1
top level: Lysis
access: public
definition: https://synbiohub.org/public/igem/BBa_K143012/1

BBa_K836008
type: Component
persistent identity: component2187598
display id: component2187598
version: 1
top level: Lysis
access: public
definition: https://synbiohub.org/public/igem/BBa_K836008/1

BBa_B1006
type: Component
persistent identity: component2187628
display id: component2187628
version: 1
top level: Lysis
access: public
definition: https://synbiohub.org/public/igem/BBa_B1006/1

BBa_K143012
type: Component
persistent identity: component2187619
display id: component2187619
version: 1
top level: Lysis
access: public
definition: https://synbiohub.org/public/igem/BBa_K143012/1

BBa_B1006
type: Component
persistent identity: component2187611
display id: component2187611
version: 1
top level: Lysis
access: public
definition: https://synbiohub.org/public/igem/BBa_B1006/1

BBa_B1006
type: Component
persistent identity: component2187640
display id: component2187640
version: 1
top level: Lysis
access: public
definition: https://synbiohub.org/public/igem/BBa_B1006/1

BBa_K836007
type: Component
persistent identity: component2187635
display id: component2187635
version: 1
top level: Lysis
access: public
definition: https://synbiohub.org/public/igem/BBa_K836007/1

BBa_K836008
type: SequenceAnnotation
persistent identity: annotation2187598
display id: annotation2187598
version: 1
top level: Lysis
location: https://synbiohub.org/public/igem/BBa_K836009/annotation2187598/range2187598/1
component: BBa_K836008

https://synbiohub.org/public/igem/BBa_K836009/annotation2187598/range2187598/1
type: Range
persistent identity: range2187598
display id: range2187598
version: 1
direction: direction/reverse
top level: Lysis
start: 1
end: 115
orientation: inline

BBa_K836007
type: SequenceAnnotation
persistent identity: annotation2187635
display id: annotation2187635
version: 1
top level: Lysis
location: https://synbiohub.org/public/igem/BBa_K836009/annotation2187635/range2187635/1
component: BBa_K836007

https://synbiohub.org/public/igem/BBa_K836009/annotation2187635/range2187635/1
type: Range
persistent identity: range2187635
display id: range2187635
version: 1
direction: direction/reverse
top level: Lysis
start: 2517
end: 3497
orientation: inline

BBa_K836005
type: SequenceAnnotation
persistent identity: annotation2187602
display id: annotation2187602
version: 1
top level: Lysis
location: https://synbiohub.org/public/igem/BBa_K836009/annotation2187602/range2187602/1
component: BBa_K836005

https://synbiohub.org/public/igem/BBa_K836009/annotation2187602/range2187602/1
type: Range
persistent identity: range2187602
display id: range2187602
version: 1
direction: direction/reverse
top level: Lysis
start: 124
end: 1470
orientation: inline

BBa_B1006
type: SequenceAnnotation
persistent identity: annotation2187628
display id: annotation2187628
version: 1
top level: Lysis
location: https://synbiohub.org/public/igem/BBa_K836009/annotation2187628/range2187628/1
component: BBa_B1006

https://synbiohub.org/public/igem/BBa_K836009/annotation2187628/range2187628/1
type: Range
persistent identity: range2187628
display id: range2187628
version: 1
direction: direction/reverse
top level: Lysis
start: 2365
end: 2403
orientation: inline

BBa_K836004
type: SequenceAnnotation
persistent identity: annotation2187623
display id: annotation2187623
version: 1
top level: Lysis
location: https://synbiohub.org/public/igem/BBa_K836009/annotation2187623/range2187623/1
component: BBa_K836004

https://synbiohub.org/public/igem/BBa_K836009/annotation2187623/range2187623/1
type: Range
persistent identity: range2187623
display id: range2187623
version: 1
direction: direction/reverse
top level: Lysis
start: 2019
end: 2356
orientation: inline

BBa_B1006
type: SequenceAnnotation
persistent identity: annotation2187616
display id: annotation2187616
version: 1
top level: Lysis
location: https://synbiohub.org/public/igem/BBa_K836009/annotation2187616/range2187616/1
component: BBa_B1006

https://synbiohub.org/public/igem/BBa_K836009/annotation2187616/range2187616/1
type: Range
persistent identity: range2187616
display id: range2187616
version: 1
direction: direction/reverse
top level: Lysis
start: 1867
end: 1905
orientation: inline

BBa_K836006
type: SequenceAnnotation
persistent identity: annotation2187606
display id: annotation2187606
version: 1
top level: Lysis
location: https://synbiohub.org/public/igem/BBa_K836009/annotation2187606/range2187606/1
component: BBa_K836006

https://synbiohub.org/public/igem/BBa_K836009/annotation2187606/range2187606/1
type: Range
persistent identity: range2187606
display id: range2187606
version: 1
direction: direction/reverse
top level: Lysis
start: 1479
end: 1811
orientation: inline

BBa_B1006
type: SequenceAnnotation
persistent identity: annotation2187640
display id: annotation2187640
version: 1
top level: Lysis
location: https://synbiohub.org/public/igem/BBa_K836009/annotation2187640/range2187640/1
component: BBa_B1006

https://synbiohub.org/public/igem/BBa_K836009/annotation2187640/range2187640/1
type: Range
persistent identity: range2187640
display id: range2187640
version: 1
direction: direction/reverse
top level: Lysis
start: 3506
end: 3544
orientation: inline

BBa_K143012
type: SequenceAnnotation
persistent identity: annotation2187619
display id: annotation2187619
version: 1
top level: Lysis
location: https://synbiohub.org/public/igem/BBa_K836009/annotation2187619/range2187619/1
component: BBa_K143012

https://synbiohub.org/public/igem/BBa_K836009/annotation2187619/range2187619/1
type: Range
persistent identity: range2187619
display id: range2187619
version: 1
direction: direction/reverse
top level: Lysis
start: 1914
end: 2010
orientation: inline

BBa_B1006
type: SequenceAnnotation
persistent identity: annotation2187611
display id: annotation2187611
version: 1
top level: Lysis
location: https://synbiohub.org/public/igem/BBa_K836009/annotation2187611/range2187611/1
component: BBa_B1006

https://synbiohub.org/public/igem/BBa_K836009/annotation2187611/range2187611/1
type: Range
persistent identity: range2187611
display id: range2187611
version: 1
direction: direction/reverse
top level: Lysis
start: 1820
end: 1858
orientation: inline

BBa_K143012
type: SequenceAnnotation
persistent identity: annotation2187631
display id: annotation2187631
version: 1
top level: Lysis
location: https://synbiohub.org/public/igem/BBa_K836009/annotation2187631/range2187631/1
component: BBa_K143012

https://synbiohub.org/public/igem/BBa_K836009/annotation2187631/range2187631/1
type: Range
persistent identity: range2187631
display id: range2187631
version: 1
direction: direction/reverse
top level: Lysis
start: 2412
end: 2508
orientation: inline

BBa_K108001
type: ComponentDefinition
persistent identity: BBa K108001
display id: BBa_K108001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: SRRz
creator: Qi Liu
created: 2008-10-14T11:00:00Z
modified: 2015-05-08T01:09:03Z
discontinued: false
dominant: false
experience: experience/None
group u list: _222_
m user id: 0
owner id: 2671
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: SRRz
mutable notes: ...
mutable provenance: Alcaligenes eutrophus H16
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Rz
sequence annotation: R
sequence annotation: S
sequence: https://synbiohub.org/public/igem/BBa_K108001_sequence/1

Rz
type: SequenceAnnotation
persistent identity: annotation1986692
display id: annotation1986692
version: 1
top level: BBa_K108001
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K108001/annotation1986692/range1986692/1

https://synbiohub.org/public/igem/BBa_K108001/annotation1986692/range1986692/1
type: Range
persistent identity: range1986692
display id: range1986692
version: 1
direction: direction/forward
top level: BBa_K108001
start: 781
end: 1239
orientation: inline

R
type: SequenceAnnotation
persistent identity: annotation1986663
display id: annotation1986663
version: 1
top level: BBa_K108001
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K108001/annotation1986663/range1986663/1

https://synbiohub.org/public/igem/BBa_K108001/annotation1986663/range1986663/1
type: Range
persistent identity: range1986663
display id: range1986663
version: 1
direction: direction/forward
top level: BBa_K108001
start: 308
end: 781
orientation: inline

S
type: SequenceAnnotation
persistent identity: annotation1986662
display id: annotation1986662
version: 1
top level: BBa_K108001
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K108001/annotation1986662/range1986662/1

https://synbiohub.org/public/igem/BBa_K108001/annotation1986662/range1986662/1
type: Range
persistent identity: range1986662
display id: range1986662
version: 1
direction: direction/forward
top level: BBa_K108001
start: 1
end: 321
orientation: inline

BBa_K124017
type: ComponentDefinition
persistent identity: BBa K124017
display id: BBa_K124017
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Bacteriophage Lysis Cassette S105, R, and Rz
creator: Neil J. Parikh
created: 2008-10-27T12:00:00Z
modified: 2015-05-08T01:09:44Z
discontinued: false
dominant: false
experience: experience/None
group u list: _221_
m user id: 0
owner id: 3365
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: The gene cassette consists of the S105 protein (Holin), R protein (Endolysin), and Rz protein. When paired with an inducible promoter, the cassette is activated by the inducer and begins to produce the proteins which lyse the cell. This cassette contains the mutant S gene, which can be found individually as BBa_K124014. The S variation allows for faster lysis.
mutable notes: None-
mutable provenance: The DNA for this part was graciously donated to the Brown iGEM Team by Ry Young at Texas A&M University. The team then BioBricked the part for submission to the registry.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K124017_sequence/1

BBa_K608352
type: ComponentDefinition
persistent identity: BBa K608352
display id: BBa_K608352
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Bacteriophage Lysis Cassette with RBS
creator: Theodhor Tiko
created: 2011-09-13T11:00:00Z
modified: 2015-05-08T01:12:52Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _780_
m user id: 0
owner id: 9113
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: RBS + Lysis Genes
mutable notes: sdf
mutable provenance: sdf
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
sequence annotation: Rz Protein
sequence annotation: S' Protein (Holin) / S Protein (Anti-holin)
sequence annotation: Rz1 Protein
sequence annotation: SpeI, XbaI scar
sequence annotation: R Protein (Endolysin: Phage lambda lysozyme)
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K608352_sequence/1

Rz Protein
type: SequenceAnnotation
persistent identity: annotation2131076
display id: annotation2131076
version: 1
top level: BBa_K608352
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K608352/annotation2131076/range2131076/1

https://synbiohub.org/public/igem/BBa_K608352/annotation2131076/range2131076/1
type: Range
persistent identity: range2131076
display id: range2131076
version: 1
direction: direction/forward
top level: BBa_K608352
start: 795
end: 1256
orientation: inline

S' Protein (Holin) / S Protein (Anti-holin)
type: SequenceAnnotation
persistent identity: annotation2131074
display id: annotation2131074
version: 1
top level: BBa_K608352
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K608352/annotation2131074/range2131074/1

https://synbiohub.org/public/igem/BBa_K608352/annotation2131074/range2131074/1
type: Range
persistent identity: range2131074
display id: range2131074
version: 1
direction: direction/forward
top level: BBa_K608352
start: 21
end: 338
orientation: inline

Rz1 Protein
type: SequenceAnnotation
persistent identity: annotation2131764
display id: annotation2131764
version: 1
top level: BBa_K608352
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K608352/annotation2131764/range2131764/1

https://synbiohub.org/public/igem/BBa_K608352/annotation2131764/range2131764/1
type: Range
persistent identity: range2131764
display id: range2131764
version: 1
direction: direction/forward
top level: BBa_K608352
start: 1015
end: 1197
orientation: inline

SpeI, XbaI scar
type: SequenceAnnotation
persistent identity: annotation2131073
display id: annotation2131073
version: 1
top level: BBa_K608352
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K608352/annotation2131073/range2131073/1

https://synbiohub.org/public/igem/BBa_K608352/annotation2131073/range2131073/1
type: Range
persistent identity: range2131073
display id: range2131073
version: 1
direction: direction/forward
top level: BBa_K608352
start: 13
end: 20
orientation: inline

R Protein (Endolysin: Phage lambda lysozyme)
type: SequenceAnnotation
persistent identity: annotation2131075
display id: annotation2131075
version: 1
top level: BBa_K608352
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K608352/annotation2131075/range2131075/1

https://synbiohub.org/public/igem/BBa_K608352/annotation2131075/range2131075/1
type: Range
persistent identity: range2131075
display id: range2131075
version: 1
direction: direction/forward
top level: BBa_K608352
start: 322
end: 798
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2131072
display id: annotation2131072
version: 1
top level: BBa_K608352
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K608352/annotation2131072/range2131072/1

https://synbiohub.org/public/igem/BBa_K608352/annotation2131072/range2131072/1
type: Range
persistent identity: range2131072
display id: range2131072
version: 1
direction: direction/forward
top level: BBa_K608352
start: 1
end: 12
orientation: inline

BBa_K358004
type: ComponentDefinition
persistent identity: BBa K358004
display id: BBa_K358004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: lambda lysis cassette[SRRz/Rz1]
creator: Ken Kajita
created: 2010-09-18T11:00:00Z
modified: 2015-05-08T01:12:12Z
discontinued: false
dominant: false
group u list: _455_
m user id: 0
owner id: 7170
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This part causes cell death. It contains holin/antiholin, endolysin and Rz/Rz1 genes.
mutable notes: We made point mutation and prepared wild type phage DNA.
mutable provenance: λ -Hind Ⅲ digest (product of TaKaRa Bio)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
sequence annotation: endolysin
sequence annotation: Rz1
sequence annotation: wt-RBS
sequence annotation: holin
sequence annotation: antiholin
sequence annotation: Rz
sequence: https://synbiohub.org/public/igem/BBa_K358004_sequence/1

endolysin
type: SequenceAnnotation
persistent identity: annotation2080449
display id: annotation2080449
version: 1
top level: BBa_K358004
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K358004/annotation2080449/range2080449/1

https://synbiohub.org/public/igem/BBa_K358004/annotation2080449/range2080449/1
type: Range
persistent identity: range2080449
display id: range2080449
version: 1
direction: direction/forward
top level: BBa_K358004
start: 336
end: 812
orientation: inline

Rz1
type: SequenceAnnotation
persistent identity: annotation2080451
display id: annotation2080451
version: 1
top level: BBa_K358004
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K358004/annotation2080451/range2080451/1

https://synbiohub.org/public/igem/BBa_K358004/annotation2080451/range2080451/1
type: Range
persistent identity: range2080451
display id: range2080451
version: 1
direction: direction/forward
top level: BBa_K358004
start: 1029
end: 1211
orientation: inline

wt-RBS
type: SequenceAnnotation
persistent identity: annotation2080446
display id: annotation2080446
version: 1
top level: BBa_K358004
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K358004/annotation2080446/range2080446/1

https://synbiohub.org/public/igem/BBa_K358004/annotation2080446/range2080446/1
type: Range
persistent identity: range2080446
display id: range2080446
version: 1
direction: direction/forward
top level: BBa_K358004
start: 2
end: 26
orientation: inline

holin
type: SequenceAnnotation
persistent identity: annotation2080448
display id: annotation2080448
version: 1
top level: BBa_K358004
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K358004/annotation2080448/range2080448/1

https://synbiohub.org/public/igem/BBa_K358004/annotation2080448/range2080448/1
type: Range
persistent identity: range2080448
display id: range2080448
version: 1
direction: direction/forward
top level: BBa_K358004
start: 35
end: 352
orientation: inline

antiholin
type: SequenceAnnotation
persistent identity: annotation2080447
display id: annotation2080447
version: 1
top level: BBa_K358004
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K358004/annotation2080447/range2080447/1

https://synbiohub.org/public/igem/BBa_K358004/annotation2080447/range2080447/1
type: Range
persistent identity: range2080447
display id: range2080447
version: 1
direction: direction/forward
top level: BBa_K358004
start: 29
end: 352
orientation: inline

Rz
type: SequenceAnnotation
persistent identity: annotation2080450
display id: annotation2080450
version: 1
top level: BBa_K358004
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K358004/annotation2080450/range2080450/1

https://synbiohub.org/public/igem/BBa_K358004/annotation2080450/range2080450/1
type: Range
persistent identity: range2080450
display id: range2080450
version: 1
direction: direction/forward
top level: BBa_K358004
start: 809
end: 1270
orientation: inline

gpS
type: ComponentDefinition
persistent identity: BBa K836006
display id: BBa_K836006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Lysis protein S from Enterobacteria phage lambda (codon usage optimized for R. opacus)
creator: Ricardo Alvarado
created: 2012-09-19T11:00:00Z
modified: 2015-05-08T01:13:32Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1095_
m user id: 0
owner id: 11819
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This gene codifies for a protein that makes holes in the inner membrane of the bacterial cell. A Gram positive RBS (SpoVG, http://partsregistry.org/wiki/index.php?title=Part:BBa_K143021) was added at the beginning.
mutable notes: Between the RBS and the CDS a spacer consisting on a scar from a standard suffix and a preffix for CDS was inserted.
mutable provenance: The amino sequence was obtained from Uniprot (http://www.uniprot.org/uniprot/P03705#section_seq lysis protein S) and optimized for R. opacus (avoiding standard restriction sites) using the free software GeneDesigner from DNA 2.0.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
sequence annotation: SpoVG
sequence annotation: Holin
sequence annotation: Spacer
sequence: https://synbiohub.org/public/igem/BBa_K836006_sequence/1

SpoVG
type: SequenceAnnotation
persistent identity: annotation2187525
display id: annotation2187525
version: 1
top level: gpS
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K836006/annotation2187525/range2187525/1

https://synbiohub.org/public/igem/BBa_K836006/annotation2187525/range2187525/1
type: Range
persistent identity: range2187525
display id: range2187525
version: 1
direction: direction/forward
top level: gpS
start: 1
end: 12
orientation: inline

Holin
type: SequenceAnnotation
persistent identity: annotation2187528
display id: annotation2187528
version: 1
top level: gpS
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K836006/annotation2187528/range2187528/1

https://synbiohub.org/public/igem/BBa_K836006/annotation2187528/range2187528/1
type: Range
persistent identity: range2187528
display id: range2187528
version: 1
direction: direction/forward
top level: gpS
start: 19
end: 333
orientation: inline

Spacer
type: SequenceAnnotation
persistent identity: annotation2187527
display id: annotation2187527
version: 1
top level: gpS
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K836006/annotation2187527/range2187527/1

https://synbiohub.org/public/igem/BBa_K836006/annotation2187527/range2187527/1
type: Range
persistent identity: range2187527
display id: range2187527
version: 1
direction: direction/forward
top level: gpS
start: 13
end: 18
orientation: inline

BBa_K117000
type: ComponentDefinition
persistent identity: BBa K117000
display id: BBa_K117000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Lysis gene (promotes lysis in colicin-producing bacteria strain)
creator: Nguyen Xuan Hung
created: 2008-10-06T11:00:00Z
modified: 2015-05-08T01:09:34Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _209_
m user id: 0
owner id: 2774
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: This lysis gene encodes for the lysis protein in colicin-producing strains of bacteria. Once activated, it causes the host cell to lyze. It also removes the immunity protein out of colicin, and hence, activates the endonuclease activity of the colicin.
mutable notes: to be updated
mutable provenance: to be updated
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K117000_sequence/1

LysA
type: ComponentDefinition
persistent identity: BBa K284001
display id: BBa_K284001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Lysozyme from Gallus gallus
creator: Tais Sineiro Herig, Raissa Estrela Curado and Gleidson Silva Teixeira
created: 2009-09-25T11:00:00Z
modified: 2015-05-08T01:11:48Z
discontinued: false
dominant: false
experience: experience/None
group u list: _386_
m user id: 0
owner id: 4968
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: .
mutable notes: .
mutable provenance: .
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: primer_binding
sequence annotation: Lysozyme
sequence annotation: Stop
sequence annotation: Start
sequence: https://synbiohub.org/public/igem/BBa_K284001_sequence/1

primer_binding
type: SequenceAnnotation
persistent identity: annotation2037722
display id: annotation2037722
version: 1
top level: LysA
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K284001/annotation2037722/range2037722/1

https://synbiohub.org/public/igem/BBa_K284001/annotation2037722/range2037722/1
type: Range
persistent identity: range2037722
display id: range2037722
version: 1
direction: direction/forward
top level: LysA
start: 1
end: 22
orientation: inline

Lysozyme
type: SequenceAnnotation
persistent identity: annotation2037720
display id: annotation2037720
version: 1
top level: LysA
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K284001/annotation2037720/range2037720/1

https://synbiohub.org/public/igem/BBa_K284001/annotation2037720/range2037720/1
type: Range
persistent identity: range2037720
display id: range2037720
version: 1
direction: direction/forward
top level: LysA
start: 1
end: 444
orientation: inline

Stop
type: SequenceAnnotation
persistent identity: annotation2037723
display id: annotation2037723
version: 1
top level: LysA
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K284001/annotation2037723/range2037723/1

https://synbiohub.org/public/igem/BBa_K284001/annotation2037723/range2037723/1
type: Range
persistent identity: range2037723
display id: range2037723
version: 1
direction: direction/forward
top level: LysA
start: 442
end: 444
orientation: inline

Start
type: SequenceAnnotation
persistent identity: annotation2037721
display id: annotation2037721
version: 1
top level: LysA
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K284001/annotation2037721/range2037721/1

https://synbiohub.org/public/igem/BBa_K284001/annotation2037721/range2037721/1
type: Range
persistent identity: range2037721
display id: range2037721
version: 1
direction: direction/forward
top level: LysA
start: 1
end: 3
orientation: inline

primer_binding
type: SequenceAnnotation
persistent identity: annotation2037724
display id: annotation2037724
version: 1
top level: LysA
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K284001/annotation2037724/range2037724/1

https://synbiohub.org/public/igem/BBa_K284001/annotation2037724/range2037724/1
type: Range
persistent identity: range2037724
display id: range2037724
version: 1
direction: direction/reverse
top level: LysA
start: 520
end: 539
orientation: inline

gpS
type: ComponentDefinition
persistent identity: BBa K836004
display id: BBa_K836004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Lysis inhibitor from Enterobacteria phage lambda (codon usage optimized for R. opacus)
creator: Ricardo Alvarado
created: 2012-09-19T11:00:00Z
modified: 2015-05-08T01:13:32Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1095_
m user id: 0
owner id: 11819
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This protein is the one in charge to avoid the formation of pores in the inner membrane of the bacterial cell. It has been optimized for expression in R. opacus. A weak RBS for Gram positive bacteria was used (http://partsregistry.org/Part:BBa_K090506:Design)in order to obtain low concentration of the protein.
mutable notes: We wanted a low concentration of this protein since medium to large concentrations could affect or completely inhibit the lysis device. Basal but constitutive expression of this protein is needed to avoid the formation of pores in the design lysis device.
mutable provenance: The amino sequence was obtained from Uniprot (http://www.uniprot.org/uniprot/P03705#section_seq lysis inhibitor isoform). The codon optimization for R. opacus (avoiding standard restriction sites EcoRI, PstI, XbaI and SpeI) was made using the free software GeneDesigner from DNA 2.0.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
sequence annotation: BBa_K090506
sequence annotation: Scar
sequence annotation: Lambda antiholin
sequence: https://synbiohub.org/public/igem/BBa_K836004_sequence/1

BBa_K090506
type: SequenceAnnotation
persistent identity: annotation2187515
display id: annotation2187515
version: 1
top level: gpS
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K836004/annotation2187515/range2187515/1

https://synbiohub.org/public/igem/BBa_K836004/annotation2187515/range2187515/1
type: Range
persistent identity: range2187515
display id: range2187515
version: 1
direction: direction/forward
top level: gpS
start: 1
end: 11
orientation: inline

Scar
type: SequenceAnnotation
persistent identity: annotation2187516
display id: annotation2187516
version: 1
top level: gpS
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K836004/annotation2187516/range2187516/1

https://synbiohub.org/public/igem/BBa_K836004/annotation2187516/range2187516/1
type: Range
persistent identity: range2187516
display id: range2187516
version: 1
direction: direction/forward
top level: gpS
start: 12
end: 17
orientation: inline

Lambda antiholin
type: SequenceAnnotation
persistent identity: annotation2187517
display id: annotation2187517
version: 1
top level: gpS
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K836004/annotation2187517/range2187517/1

https://synbiohub.org/public/igem/BBa_K836004/annotation2187517/range2187517/1
type: Range
persistent identity: range2187517
display id: range2187517
version: 1
direction: direction/forward
top level: gpS
start: 18
end: 338
orientation: inline