//cds/membrane/channel
type: Collection
persistent identity: cds membrane channel
display id: cds_membrane_channel
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
owned by: james
owned by: myers
member: BBa_K1020009
member: BBa_K1125000
member: BBa_K090000
member: ExbB
member: BBa_K559000
member: BBa_K864204
member: BBa_K772102
member: BBa_I746200
member: BBa_K559010
member: BBa_K284000
member: PrtDEF
member: TonB
member: BBa_K559001

BBa_K864204
type: ComponentDefinition
persistent identity: BBa K864204
display id: BBa_K864204
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: OmpF
creator: Erik Lundin
created: 2012-09-24T11:00:00Z
modified: 2015-05-08T01:13:37Z
discontinued: false
dominant: false
group u list: _1124_
m user id: 0
owner id: 9827
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: OmpF
mutable notes: Illegal PstI restriction site removed by silent mutations T>A at position 66 and A>T at position 69 using primers listed below (annealing sequences are underlined) ompF_rsr_F CTGAAATCTATAACAAAGATGGCAA ompF_rsr_R CTGCGTTTGCAGTACCTGCT
mutable provenance: Amplified from MG1655 (accession NC_000913) chromosome using primers listed below (annealing sequences are underlined) ompF_BB_F TTCGAATTCGCGGCCGCTTCTAGATGATGAAGCGCAATATTC ompF_BB_R CGGCCGCTACTAGTATTATTAGAACTGGTAAACGATACC
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: A>T
sequence annotation: T>A
sequence annotation: ompF
sequence: https://synbiohub.org/public/igem/BBa_K864204_sequence/1

A>T
type: SequenceAnnotation
persistent identity: annotation2197201
display id: annotation2197201
version: 1
top level: BBa_K864204
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_K864204/annotation2197201/range2197201/1

https://synbiohub.org/public/igem/BBa_K864204/annotation2197201/range2197201/1
type: Range
persistent identity: range2197201
display id: range2197201
version: 1
direction: direction/forward
top level: BBa_K864204
start: 69
end: 69
orientation: inline

T>A
type: SequenceAnnotation
persistent identity: annotation2197200
display id: annotation2197200
version: 1
top level: BBa_K864204
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_K864204/annotation2197200/range2197200/1

https://synbiohub.org/public/igem/BBa_K864204/annotation2197200/range2197200/1
type: Range
persistent identity: range2197200
display id: range2197200
version: 1
direction: direction/forward
top level: BBa_K864204
start: 66
end: 66
orientation: inline

ompF
type: SequenceAnnotation
persistent identity: annotation2197199
display id: annotation2197199
version: 1
top level: BBa_K864204
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K864204/annotation2197199/range2197199/1

https://synbiohub.org/public/igem/BBa_K864204/annotation2197199/range2197199/1
type: Range
persistent identity: range2197199
display id: range2197199
version: 1
direction: direction/forward
top level: BBa_K864204
start: 1
end: 1092
orientation: inline

BBa_K559010
type: ComponentDefinition
persistent identity: BBa K559010
display id: BBa_K559010
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Halorhodopsin complete system
creator: Jacky, Fong Chuen, Loo
created: 2011-09-28T11:00:00Z
modified: 2015-05-08T01:12:41Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _727_
m user id: 0
owner id: 6123
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: Halorhodopsin gene allow chloride ion to be pumped in by the level of light intensity. Halorhodopsin gene is under regulation by constitute T7 promoter to allow protein coding to be maximized, and the bidirectional terminator is added for high efficiency termination of both forward and backward transcription
mutable notes: The double terminator wass tested to stop effectively on the transcription of both forward and backward transcription to make our protein generator be a controlled system
mutable provenance: Halorhodopsin comes from part BBa_K559000, which is come from genomic sequence of N.pharaonis (genebank ID:J05199.1)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K559001
component: BBa_B0014
sequence annotation: BBa_K559001
sequence annotation: BBa_B0014
sequence: https://synbiohub.org/public/igem/BBa_K559010_sequence/1

BBa_K559001
type: Component
persistent identity: component2177574
display id: component2177574
version: 1
top level: BBa_K559010
access: public
definition: BBa_K559001

BBa_B0014
type: Component
persistent identity: component2177581
display id: component2177581
version: 1
top level: BBa_K559010
access: public
definition: https://synbiohub.org/public/igem/BBa_B0014/1

BBa_K559001
type: SequenceAnnotation
persistent identity: annotation2177574
display id: annotation2177574
version: 1
top level: BBa_K559010
location: https://synbiohub.org/public/igem/BBa_K559010/annotation2177574/range2177574/1
component: BBa_K559001

https://synbiohub.org/public/igem/BBa_K559010/annotation2177574/range2177574/1
type: Range
persistent identity: range2177574
display id: range2177574
version: 1
direction: direction/reverse
top level: BBa_K559010
start: 1
end: 728
orientation: inline

BBa_B0014
type: SequenceAnnotation
persistent identity: annotation2177581
display id: annotation2177581
version: 1
top level: BBa_K559010
location: https://synbiohub.org/public/igem/BBa_K559010/annotation2177581/range2177581/1
component: BBa_B0014

https://synbiohub.org/public/igem/BBa_K559010/annotation2177581/range2177581/1
type: Range
persistent identity: range2177581
display id: range2177581
version: 1
direction: direction/reverse
top level: BBa_K559010
start: 737
end: 831
orientation: inline

TonB
type: ComponentDefinition
persistent identity: BBa I765003
display id: BBa_I765003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TonB ferric siderophore transport system, periplasmic binding protein TonB [ Pseudomonas entomophila
creator: iGEM Colombian Team 2007
created: 2007-10-25T11:00:00Z
modified: 2015-08-31T04:08:09Z
discontinued: false
dominant: false
experience: experience/None
group u list: _118_
m user id: 0
owner id: 2266
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This device codify for TonB, a transmembranal Iron channel that appears to transduce energy between the cytoplasmic and the outer membrane in Escherichia coli.
mutable notes: None
mutable provenance: Genomic Sequence
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: rbs
sequence annotation: protein
sequence: https://synbiohub.org/public/igem/BBa_I765003_sequence/1

rbs
type: SequenceAnnotation
persistent identity: annotation1956935
display id: annotation1956935
version: 1
top level: TonB
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_I765003/annotation1956935/range1956935/1

https://synbiohub.org/public/igem/BBa_I765003/annotation1956935/range1956935/1
type: Range
persistent identity: range1956935
display id: range1956935
version: 1
direction: direction/forward
top level: TonB
start: 1
end: 10
orientation: inline

protein
type: SequenceAnnotation
persistent identity: annotation1956936
display id: annotation1956936
version: 1
top level: TonB
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_I765003/annotation1956936/range1956936/1

https://synbiohub.org/public/igem/BBa_I765003/annotation1956936/range1956936/1
type: Range
persistent identity: range1956936
display id: range1956936
version: 1
direction: direction/forward
top level: TonB
start: 12
end: 735
orientation: inline

BBa_K090000
type: ComponentDefinition
persistent identity: BBa K090000
display id: BBa_K090000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Glutamate gated K+ channel
creator: Ellis O'Neill
created: 2008-10-22T11:00:00Z
modified: 2015-05-08T01:08:37Z
discontinued: false
dominant: false
group u list: _188_
m user id: 0
owner id: 3483
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part encodes the gene GluR0 from Synechocystis. It is a well characterised glutamate-gated K+ membrane channel, which has a considerable degree of structural and functional homology to rat neurone GluR2 AMPA receptors.
mutable notes: From peptide sequence of Synechocystis PCC 6803, codon optimised for E.coli, restriction sites removed, and synthesised de novo.
mutable provenance: From Genomic sequence
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K090000_sequence/1

BBa_K559001
type: ComponentDefinition
persistent identity: BBa K559001
display id: BBa_K559001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Halorhodopsin under T7 promoter
creator: Jacky, Fong Chuen, Loo
created: 2011-09-28T11:00:00Z
modified: 2015-05-08T01:12:41Z
discontinued: false
dominant: false
experience: experience/None
group u list: _727_
m user id: 0
owner id: 6123
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Halorhodopsin under constitute T7 promoter to regulate the gene transcription to be maximized and no restrict to other regulatory factor
mutable notes: The coding sequence of N.pharaonis J05199.1 is selected from 61nt to 700nt and the T7 promotor placed in front by 3A assembly
mutable provenance: Halorhodopsin come from N.pharaonis, gene bank J05199.1
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: RBS
sequence annotation: T7 promotor
sequence annotation: Halorhodopsin
sequence annotation: lac repressor
sequence: https://synbiohub.org/public/igem/BBa_K559001_sequence/1

RBS
type: SequenceAnnotation
persistent identity: annotation2177555
display id: annotation2177555
version: 1
top level: BBa_K559001
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K559001/annotation2177555/range2177555/1

https://synbiohub.org/public/igem/BBa_K559001/annotation2177555/range2177555/1
type: Range
persistent identity: range2177555
display id: range2177555
version: 1
direction: direction/forward
top level: BBa_K559001
start: 66
end: 77
orientation: inline

T7 promotor
type: SequenceAnnotation
persistent identity: annotation2177551
display id: annotation2177551
version: 1
top level: BBa_K559001
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K559001/annotation2177551/range2177551/1

https://synbiohub.org/public/igem/BBa_K559001/annotation2177551/range2177551/1
type: Range
persistent identity: range2177551
display id: range2177551
version: 1
direction: direction/forward
top level: BBa_K559001
start: 1
end: 16
orientation: inline

Halorhodopsin
type: SequenceAnnotation
persistent identity: annotation2177554
display id: annotation2177554
version: 1
top level: BBa_K559001
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K559001/annotation2177554/range2177554/1

https://synbiohub.org/public/igem/BBa_K559001/annotation2177554/range2177554/1
type: Range
persistent identity: range2177554
display id: range2177554
version: 1
direction: direction/forward
top level: BBa_K559001
start: 89
end: 728
orientation: inline

lac repressor
type: SequenceAnnotation
persistent identity: annotation2177553
display id: annotation2177553
version: 1
top level: BBa_K559001
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K559001/annotation2177553/range2177553/1

https://synbiohub.org/public/igem/BBa_K559001/annotation2177553/range2177553/1
type: Range
persistent identity: range2177553
display id: range2177553
version: 1
direction: direction/forward
top level: BBa_K559001
start: 22
end: 42
orientation: inline

BBa_K284000
type: ComponentDefinition
persistent identity: BBa K284000
display id: BBa_K284000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Lactate Permease from Kluyveromyces lactis
creator: Ra??ssa Estrela Curado, Ta??s Sineiro Herig and Gleidson Silva Teixeira
created: 2009-08-15T11:00:00Z
modified: 2015-05-08T01:11:48Z
discontinued: false
dominant: false
experience: experience/None
group u list: _386_
m user id: 0
owner id: 4968
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: JEN1 from Kluyveromyces lactis is a homologue gene to JEN1 of Saccharomyces cerevisiae. It ecodes a monocarboxylate acids permease shared by lactic and pyruvic acids. Queiros O, Pereira L, Paiva S, Moradas-Ferreira P and Casal M. 2007. Functional analysis of Kluyveromyces lactis carboxylic acids permeases: heterologous expression of KlJEN1 and KlJEN2 genes. Curr Genet 51, 161-169.
mutable notes: Working yet.
mutable provenance: This part was amplified by PCR from K. lactis genomic DNA.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: primer_binding
sequence annotation: Stop
sequence annotation: Start
sequence annotation: Lactate Permease
sequence: https://synbiohub.org/public/igem/BBa_K284000_sequence/1

primer_binding
type: SequenceAnnotation
persistent identity: annotation2027259
display id: annotation2027259
version: 1
top level: BBa_K284000
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K284000/annotation2027259/range2027259/1

https://synbiohub.org/public/igem/BBa_K284000/annotation2027259/range2027259/1
type: Range
persistent identity: range2027259
display id: range2027259
version: 1
direction: direction/forward
top level: BBa_K284000
start: 1
end: 21
orientation: inline

Stop
type: SequenceAnnotation
persistent identity: annotation2027262
display id: annotation2027262
version: 1
top level: BBa_K284000
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K284000/annotation2027262/range2027262/1

https://synbiohub.org/public/igem/BBa_K284000/annotation2027262/range2027262/1
type: Range
persistent identity: range2027262
display id: range2027262
version: 1
direction: direction/forward
top level: BBa_K284000
start: 1807
end: 1809
orientation: inline

primer_binding
type: SequenceAnnotation
persistent identity: annotation2027263
display id: annotation2027263
version: 1
top level: BBa_K284000
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K284000/annotation2027263/range2027263/1

https://synbiohub.org/public/igem/BBa_K284000/annotation2027263/range2027263/1
type: Range
persistent identity: range2027263
display id: range2027263
version: 1
direction: direction/reverse
top level: BBa_K284000
start: 1855
end: 1873
orientation: inline

Start
type: SequenceAnnotation
persistent identity: annotation2027261
display id: annotation2027261
version: 1
top level: BBa_K284000
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K284000/annotation2027261/range2027261/1

https://synbiohub.org/public/igem/BBa_K284000/annotation2027261/range2027261/1
type: Range
persistent identity: range2027261
display id: range2027261
version: 1
direction: direction/forward
top level: BBa_K284000
start: 7
end: 9
orientation: inline

Lactate Permease
type: SequenceAnnotation
persistent identity: annotation2027260
display id: annotation2027260
version: 1
top level: BBa_K284000
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K284000/annotation2027260/range2027260/1

https://synbiohub.org/public/igem/BBa_K284000/annotation2027260/range2027260/1
type: Range
persistent identity: range2027260
display id: range2027260
version: 1
direction: direction/forward
top level: BBa_K284000
start: 7
end: 1809
orientation: inline

BBa_K1020009
type: ComponentDefinition
persistent identity: BBa K1020009
display id: BBa_K1020009
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: YbhGFSR operon
creator: Yue Liu
created: 2013-09-09T11:00:00Z
modified: 2015-05-08T01:08:43Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1327_
m user id: 0
owner id: 16881
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: ybhGFSR operon encoding an ABC efflux pump
mutable notes: a complete sequence
mutable provenance: E.coli MG1655
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_J23100
component: BBa_K1020006
component: BBa_B0034
sequence annotation: BBa_J23100
sequence annotation: BBa_K1020006
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K1020009_sequence/1

BBa_J23100
type: Component
persistent identity: component2338214
display id: component2338214
version: 1
top level: BBa_K1020009
access: public
definition: https://synbiohub.org/public/igem/BBa_J23100/1

BBa_K1020006
type: Component
persistent identity: component2338217
display id: component2338217
version: 1
top level: BBa_K1020009
access: public
definition: https://synbiohub.org/public/igem/BBa_K1020006/1

BBa_B0034
type: Component
persistent identity: component2338216
display id: component2338216
version: 1
top level: BBa_K1020009
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_J23100
type: SequenceAnnotation
persistent identity: annotation2338214
display id: annotation2338214
version: 1
top level: BBa_K1020009
location: https://synbiohub.org/public/igem/BBa_K1020009/annotation2338214/range2338214/1
component: BBa_J23100

https://synbiohub.org/public/igem/BBa_K1020009/annotation2338214/range2338214/1
type: Range
persistent identity: range2338214
display id: range2338214
version: 1
direction: direction/reverse
top level: BBa_K1020009
start: 1
end: 35
orientation: inline

BBa_K1020006
type: SequenceAnnotation
persistent identity: annotation2338217
display id: annotation2338217
version: 1
top level: BBa_K1020009
location: https://synbiohub.org/public/igem/BBa_K1020009/annotation2338217/range2338217/1
component: BBa_K1020006

https://synbiohub.org/public/igem/BBa_K1020009/annotation2338217/range2338217/1
type: Range
persistent identity: range2338217
display id: range2338217
version: 1
direction: direction/reverse
top level: BBa_K1020009
start: 64
end: 5034
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2338216
display id: annotation2338216
version: 1
top level: BBa_K1020009
location: https://synbiohub.org/public/igem/BBa_K1020009/annotation2338216/range2338216/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1020009/annotation2338216/range2338216/1
type: Range
persistent identity: range2338216
display id: range2338216
version: 1
direction: direction/reverse
top level: BBa_K1020009
start: 44
end: 55
orientation: inline

BBa_K559000
type: ComponentDefinition
persistent identity: BBa K559000
display id: BBa_K559000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Halorhodopsin
creator: Jacky, Fong Chuen, Loo
created: 2011-09-28T11:00:00Z
modified: 2015-05-08T01:12:41Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _727_
m user id: 0
owner id: 6123
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This Halorhodopsin gene allow the photoreceptor protein be expressed, to regulate the chloride ion pumped in by light intensity
mutable notes: The DNA sequence selected from ID J05199.1 of 61nt to 700nt for protein coding
mutable provenance: It is from N.pharaonis, gene back ID J05199.1
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Halorhodopsin
sequence: https://synbiohub.org/public/igem/BBa_K559000_sequence/1

Halorhodopsin
type: SequenceAnnotation
persistent identity: annotation2147609
display id: annotation2147609
version: 1
top level: BBa_K559000
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K559000/annotation2147609/range2147609/1

https://synbiohub.org/public/igem/BBa_K559000/annotation2147609/range2147609/1
type: Range
persistent identity: range2147609
display id: range2147609
version: 1
direction: direction/forward
top level: BBa_K559000
start: 1
end: 640
orientation: inline

BBa_K1125000
type: ComponentDefinition
persistent identity: BBa K1125000
display id: BBa_K1125000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: SrUCPa w/ kozack sequence
creator: Tsung-Yu, Lu
created: 2013-09-15T11:00:00Z
modified: 2015-05-08T01:09:18Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1437_
m user id: 0
owner id: 17654
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: SrUCPa, a human UCP1 homologue, is a mitocondrial inner membrane protein of skunk cabbage, Symplocarpus renifolius. It facilitates proton leaks across the mitocondrial inner membrane, which confers skunk cabbages the ability to produce heat under low temperature.
mutable notes: presence of an invalid XbaI site
mutable provenance: coming from genomic DNA of skunk cabbage, Symplocarpus renifolius.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
sequence annotation: Kozack sequence
sequence annotation: stop codon
sequence annotation: start codon
sequence annotation: SrUCP
sequence: https://synbiohub.org/public/igem/BBa_K1125000_sequence/1

Kozack sequence
type: SequenceAnnotation
persistent identity: annotation2346201
display id: annotation2346201
version: 1
top level: BBa_K1125000
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1125000/annotation2346201/range2346201/1

https://synbiohub.org/public/igem/BBa_K1125000/annotation2346201/range2346201/1
type: Range
persistent identity: range2346201
display id: range2346201
version: 1
direction: direction/forward
top level: BBa_K1125000
start: 1
end: 20
orientation: inline

stop codon
type: SequenceAnnotation
persistent identity: annotation2346204
display id: annotation2346204
version: 1
top level: BBa_K1125000
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1125000/annotation2346204/range2346204/1

https://synbiohub.org/public/igem/BBa_K1125000/annotation2346204/range2346204/1
type: Range
persistent identity: range2346204
display id: range2346204
version: 1
direction: direction/forward
top level: BBa_K1125000
start: 930
end: 935
orientation: inline

start codon
type: SequenceAnnotation
persistent identity: annotation2346203
display id: annotation2346203
version: 1
top level: BBa_K1125000
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1125000/annotation2346203/range2346203/1

https://synbiohub.org/public/igem/BBa_K1125000/annotation2346203/range2346203/1
type: Range
persistent identity: range2346203
display id: range2346203
version: 1
direction: direction/forward
top level: BBa_K1125000
start: 21
end: 23
orientation: inline

SrUCP
type: SequenceAnnotation
persistent identity: annotation2346202
display id: annotation2346202
version: 1
top level: BBa_K1125000
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1125000/annotation2346202/range2346202/1

https://synbiohub.org/public/igem/BBa_K1125000/annotation2346202/range2346202/1
type: Range
persistent identity: range2346202
display id: range2346202
version: 1
direction: direction/forward
top level: BBa_K1125000
start: 21
end: 935
orientation: inline

PrtDEF
type: ComponentDefinition
persistent identity: BBa K258008
display id: BBa_K258008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Export of recombinant proteins in Escherichia coli using ABC transporter of Erwinia chrysanthemi
creator: Jung Hoon Ahn from Korea Advanced Institute of Science and Technology
created: 2009-10-09T11:00:00Z
modified: 2015-05-08T01:11:42Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _358_
m user id: 0
owner id: 4260
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: EGF-TliA, EGF-LARD1 fusion proteins were secreted when ABC transporter was present. E. coli harboring the PrtDEF of E. chrysanthemi secreted more fusion proteins than without ABC transporter. In fact, in enzyme activation assay(with Hitachi Spectrophotometer) we observed that although our protein -EGF- has disulfide bonds, it was excreted succesfully. In addition, when compearing with lack of ABC domain, we saw that protein excretion into medium (supernatant) is 10-fold more with ABC transporter-PrtDEF-. PrtDEF functioned well at 37??C, the optimum growth temperature for E. coli
mutable notes: We used whole plasmid in order to excrete a fusion protein with one of two signal tags which are TliA and LARD1
mutable provenance: Sequence and Features PrtDEF: ABC transporter of Erwinia chrysanthemi for PrtA EGF: Human Epidermal Growth Factor
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000155
role: partType/Plasmid
component: BBa_K258100
component: BBa_K258101
component: BBa_B0034
component: BBa_B0010
component: BBa_B0012
component: BBa_R0040
component: BBa_K258102
sequence annotation: BBa_B0010
sequence annotation: BBa_B0034
sequence annotation: BBa_K258102
sequence annotation: BBa_R0040
sequence annotation: BBa_K258100
sequence annotation: BBa_K258101
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_K258008_sequence/1

BBa_K258100
type: Component
persistent identity: component2033394
display id: component2033394
version: 1
top level: PrtDEF
access: public
definition: https://synbiohub.org/public/igem/BBa_K258100/1

BBa_K258101
type: Component
persistent identity: component2033399
display id: component2033399
version: 1
top level: PrtDEF
access: public
definition: https://synbiohub.org/public/igem/BBa_K258101/1

BBa_B0034
type: Component
persistent identity: component2033398
display id: component2033398
version: 1
top level: PrtDEF
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0010
type: Component
persistent identity: component2033395
display id: component2033395
version: 1
top level: PrtDEF
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0034
type: Component
persistent identity: component2033393
display id: component2033393
version: 1
top level: PrtDEF
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0010
type: Component
persistent identity: component2033405
display id: component2033405
version: 1
top level: PrtDEF
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0012
type: Component
persistent identity: component2033407
display id: component2033407
version: 1
top level: PrtDEF
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_R0040
type: Component
persistent identity: component2033387
display id: component2033387
version: 1
top level: PrtDEF
access: public
definition: https://synbiohub.org/public/igem/BBa_R0040/1

BBa_K258102
type: Component
persistent identity: component2033404
display id: component2033404
version: 1
top level: PrtDEF
access: public
definition: https://synbiohub.org/public/igem/BBa_K258102/1

BBa_B0010
type: Component
persistent identity: component2033400
display id: component2033400
version: 1
top level: PrtDEF
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0034
type: Component
persistent identity: component2033403
display id: component2033403
version: 1
top level: PrtDEF
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2033405
display id: annotation2033405
version: 1
top level: PrtDEF
location: https://synbiohub.org/public/igem/BBa_K258008/annotation2033405/range2033405/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K258008/annotation2033405/range2033405/1
type: Range
persistent identity: range2033405
display id: range2033405
version: 1
direction: direction/forward
top level: PrtDEF
start: 4772
end: 4851
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2033398
display id: annotation2033398
version: 1
top level: PrtDEF
location: https://synbiohub.org/public/igem/BBa_K258008/annotation2033398/range2033398/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K258008/annotation2033398/range2033398/1
type: Range
persistent identity: range2033398
display id: range2033398
version: 1
direction: direction/reverse
top level: PrtDEF
start: 1902
end: 1913
orientation: inline

BBa_K258102
type: SequenceAnnotation
persistent identity: annotation2033404
display id: annotation2033404
version: 1
top level: PrtDEF
location: https://synbiohub.org/public/igem/BBa_K258008/annotation2033404/range2033404/1
component: BBa_K258102

https://synbiohub.org/public/igem/BBa_K258008/annotation2033404/range2033404/1
type: Range
persistent identity: range2033404
display id: range2033404
version: 1
direction: direction/reverse
top level: PrtDEF
start: 3378
end: 4763
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation2033387
display id: annotation2033387
version: 1
top level: PrtDEF
location: https://synbiohub.org/public/igem/BBa_K258008/annotation2033387/range2033387/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_K258008/annotation2033387/range2033387/1
type: Range
persistent identity: range2033387
display id: range2033387
version: 1
direction: direction/forward
top level: PrtDEF
start: 1
end: 54
orientation: inline

BBa_K258100
type: SequenceAnnotation
persistent identity: annotation2033394
display id: annotation2033394
version: 1
top level: PrtDEF
location: https://synbiohub.org/public/igem/BBa_K258008/annotation2033394/range2033394/1
component: BBa_K258100

https://synbiohub.org/public/igem/BBa_K258008/annotation2033394/range2033394/1
type: Range
persistent identity: range2033394
display id: range2033394
version: 1
direction: direction/reverse
top level: PrtDEF
start: 81
end: 1805
orientation: inline

BBa_K258101
type: SequenceAnnotation
persistent identity: annotation2033399
display id: annotation2033399
version: 1
top level: PrtDEF
location: https://synbiohub.org/public/igem/BBa_K258008/annotation2033399/range2033399/1
component: BBa_K258101

https://synbiohub.org/public/igem/BBa_K258008/annotation2033399/range2033399/1
type: Range
persistent identity: range2033399
display id: range2033399
version: 1
direction: direction/reverse
top level: PrtDEF
start: 1920
end: 3263
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2033400
display id: annotation2033400
version: 1
top level: PrtDEF
location: https://synbiohub.org/public/igem/BBa_K258008/annotation2033400/range2033400/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K258008/annotation2033400/range2033400/1
type: Range
persistent identity: range2033400
display id: range2033400
version: 1
direction: direction/forward
top level: PrtDEF
start: 3272
end: 3351
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2033393
display id: annotation2033393
version: 1
top level: PrtDEF
location: https://synbiohub.org/public/igem/BBa_K258008/annotation2033393/range2033393/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K258008/annotation2033393/range2033393/1
type: Range
persistent identity: range2033393
display id: range2033393
version: 1
direction: direction/reverse
top level: PrtDEF
start: 63
end: 74
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2033395
display id: annotation2033395
version: 1
top level: PrtDEF
location: https://synbiohub.org/public/igem/BBa_K258008/annotation2033395/range2033395/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K258008/annotation2033395/range2033395/1
type: Range
persistent identity: range2033395
display id: range2033395
version: 1
direction: direction/forward
top level: PrtDEF
start: 1814
end: 1893
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2033403
display id: annotation2033403
version: 1
top level: PrtDEF
location: https://synbiohub.org/public/igem/BBa_K258008/annotation2033403/range2033403/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K258008/annotation2033403/range2033403/1
type: Range
persistent identity: range2033403
display id: range2033403
version: 1
direction: direction/reverse
top level: PrtDEF
start: 3360
end: 3371
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2033407
display id: annotation2033407
version: 1
top level: PrtDEF
location: https://synbiohub.org/public/igem/BBa_K258008/annotation2033407/range2033407/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K258008/annotation2033407/range2033407/1
type: Range
persistent identity: range2033407
display id: range2033407
version: 1
direction: direction/forward
top level: PrtDEF
start: 4860
end: 4900
orientation: inline

BBa_K772102
type: ComponentDefinition
persistent identity: BBa K772102
display id: BBa_K772102
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Halorhodopsin ready-to-use system
creator: Mustafa Elitok
created: 2012-09-25T11:00:00Z
modified: 2015-05-08T01:13:15Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1024_
m user id: 0
owner id: 9573
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Halorhodopsin is an inward-directed light-driven chloride ion pump originating from Halobacterium. It utilizes light to pump chloride ions against chloride concentration difference into cells from the environment. We added a constitutive working and regulatable pLac promoter in the upstream region of the part in order to use it in our design easily.
mutable notes: Please refer to http://partsregistry.org/Part:BBa_K559000:Design for more detail about halorhodopsin. Please refer to http://partsregistry.org/Part:BBa_J04500:Design for more detail about pLac promoter.
mutable provenance: Please refer to http://partsregistry.org/Part:BBa_K559000:Design, both of the genes are gathered from the Registry distribution.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K559010
component: BBa_J04500
sequence annotation: BBa_J04500
sequence annotation: BBa_K559010
sequence: https://synbiohub.org/public/igem/BBa_K772102_sequence/1

BBa_K559010
type: Component
persistent identity: component2202534
display id: component2202534
version: 1
top level: BBa_K772102
access: public
definition: BBa_K559010

BBa_J04500
type: Component
persistent identity: component2202521
display id: component2202521
version: 1
top level: BBa_K772102
access: public
definition: https://synbiohub.org/public/igem/BBa_J04500/1

BBa_J04500
type: SequenceAnnotation
persistent identity: annotation2202521
display id: annotation2202521
version: 1
top level: BBa_K772102
location: https://synbiohub.org/public/igem/BBa_K772102/annotation2202521/range2202521/1
component: BBa_J04500

https://synbiohub.org/public/igem/BBa_K772102/annotation2202521/range2202521/1
type: Range
persistent identity: range2202521
display id: range2202521
version: 1
direction: direction/reverse
top level: BBa_K772102
start: 1
end: 220
orientation: inline

BBa_K559010
type: SequenceAnnotation
persistent identity: annotation2202534
display id: annotation2202534
version: 1
top level: BBa_K772102
location: https://synbiohub.org/public/igem/BBa_K772102/annotation2202534/range2202534/1
component: BBa_K559010

https://synbiohub.org/public/igem/BBa_K772102/annotation2202534/range2202534/1
type: Range
persistent identity: range2202534
display id: range2202534
version: 1
direction: direction/reverse
top level: BBa_K772102
start: 229
end: 1059
orientation: inline

ExbB
type: ComponentDefinition
persistent identity: BBa I765002
display id: BBa_I765002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: ExbB membrane spanning protein in TonB-ExbB-ExbD complex [ Escherichia coli K12 ]
creator: iGEM Colombian Team 2007
created: 2007-10-25T11:00:00Z
modified: 2015-08-31T04:08:09Z
discontinued: false
dominant: false
experience: experience/None
group u list: _118_
m user id: 0
owner id: 2267
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: this part codifies for a membran localized protein that transduces energy from the proton motive force to high-affinity receptors in the outer membrane. it is part of the complex composede by a TonB ExbB and ExbD
mutable notes: this part has its original ribosomal binding site
mutable provenance: genomic sequence
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: rbs
sequence annotation: protein
sequence: https://synbiohub.org/public/igem/BBa_I765002_sequence/1

rbs
type: SequenceAnnotation
persistent identity: annotation1957821
display id: annotation1957821
version: 1
top level: ExbB
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_I765002/annotation1957821/range1957821/1

https://synbiohub.org/public/igem/BBa_I765002/annotation1957821/range1957821/1
type: Range
persistent identity: range1957821
display id: range1957821
version: 1
direction: direction/forward
top level: ExbB
start: 1
end: 15
orientation: inline

protein
type: SequenceAnnotation
persistent identity: annotation1957820
display id: annotation1957820
version: 1
top level: ExbB
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_I765002/annotation1957820/range1957820/1

https://synbiohub.org/public/igem/BBa_I765002/annotation1957820/range1957820/1
type: Range
persistent identity: range1957820
display id: range1957820
version: 1
direction: direction/forward
top level: ExbB
start: 16
end: 735
orientation: inline

BBa_I746200
type: ComponentDefinition
persistent identity: BBa I746200
display id: BBa_I746200
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: FepA L8T Mutant - Large Diffusion pore for E. coli outer membrane.
creator: Narin Hengrung
created: 2007-08-28T11:00:00Z
modified: 2015-08-31T04:08:04Z
discontinued: false
dominant: false
experience: experience/None
group u list: _116_
m user id: 0
owner id: 2118
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: FepA is an outer membrane receptor protein for Escherichia coli involved in the uptake of the ferric enterobactin siderophore. This part is a deletion mutant of the wild type FepA, which has been shown to convert the protein into an open diffusion channel, similar to a porin. It allows larger molecules through than normally would be permitted.
mutable notes: The original mutants had an EcoR1 restriction site within their coding sequence. This had to be removed via site directed mutagenesis, using a megaprimer based method.
mutable provenance: The mutants were developed by Dr Salete Newton working in Professor P. Klebba's lab at the university of Oklahoma. They were kindly sent to us by Professor Klebba.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_I746200_sequence/1