//cds/membrane/lysis
type: Collection
persistent identity: cds membrane lysis
display id: cds_membrane_lysis
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
owned by: james
owned by: myers
member: BBa_K112009
member: BBa_K112306
member: BBa_K124014
member: BBa_K112004
member: BBa_K112314
member: BBa_K112310
member: BBa_K112000
member: BBa_K358004
member: BBa_K112307
member: BBa_K1639006
member: gpS
member: BBa_K1479016
member: BBa_K112315
member: BBa_K112010
member: BBa_K112002
member: BBa_K112007
member: BBa_K112006
member: BBa_K112316
member: BBa_K112308
member: BBa_K112312
member: BBa_K112313
member: Holin, T4
member: BBa_K112201
member: BBa_K608352
member: BBa_K1919100
member: BBa_K112008
member: BBa_K112309

Holin, T4
type: ComponentDefinition
persistent identity: BBa K112003
display id: BBa_K112003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: T4 holin, without start or stop codon, berkeley standard
creator: Bing Xia
created: 2008-09-02T11:00:00Z
modified: 2015-05-08T01:09:13Z
discontinued: false
dominant: false
experience: experience/None
group u list: _224_
m user id: 0
owner id: 2998
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: T4 holin gene without start or stop codons, in BBb format. ---- This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at: [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
mutable provenance: PCR bx3/bx4 on Bjh1301 (682 bp, EcoRI/BamHI/DpnI) Sub into pBca1256 (EcoRI/BamHI) Product is pBca1256-K112003 ---------------------------------------- bx3 Forward Biobricking of {<T4 Holin>} ccagtGAATTCatgAGATCTgcagcacctagaatatcattttcgccc bx4 Reverse Biobricking of {<T4 Holin>} cgttaGGATCCtttagcccttcctaatattctggcc
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence: https://synbiohub.org/public/igem/BBa_K112003_sequence/1

BBa_K1919100
type: ComponentDefinition
persistent identity: BBa K1919100
display id: BBa_K1919100
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: The device consist of temperature promotor and hokD coding gene.
creator: Zekun Lv
created: 2016-10-13T11:00:00Z
modified: 2016-10-18T09:59:58Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2386_
m user id: 27466
owner id: 27466
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This device is a combination of two parts submitted by previous igem teams that one （BBa_K608351） is a temperature-regulated promoter followed by the other （BBa_K1497008 ）that a gene coding for a protein hokD which induces cell lysis. The promoter we chose won???t start translation until the temperature is raised to 37℃. And at 42℃ can we get the peck efficiency of expression. The promoter is in fact a composite sequence that preceding the regulated promoter is the control sequence which can synthesis λ cI repressor. λ cI repressor binds with the promoter sequence when environment temperature is 28~30℃ while at 42℃, the protein denatures and falls off by which initiate the transcription. We use the temperature-regulated promoter to express hokD gene. The hokD gene of??E. coli??transcribes for a small polypeptide that causes cell death by elimination of vital cell wall functions. In short , our device can induce cell lysis when temperature is raised to 42℃. To deliver the antimicrobial peptides in the right place and at the right time, we designed and constructed this temperature-regulated device. When put under the sole of feet, our peptide-expressing E.coli cells in the insole would be incubated at a warmer environment due to body temperature. We use this promoter to control the synthesis of hokD for the raised temperature will promote the translation of hokD which following the promoter.
mutable notes: We use pSB1C3 vector to express this device for its more avaliable.
mutable provenance: we acquired that two parts BBa_K608351 and BBa_K1497008 we based on on igem distribution plates and constructed the composite device through molecular biology technique.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
sequence annotation: BBa_K608351
sequence annotation: BBa_K1497008
sequence: https://synbiohub.org/public/igem/BBa_K1919100_sequence/1

BBa_K608351
type: SequenceAnnotation
persistent identity: annotation2501464
display id: annotation2501464
version: 1
top level: BBa_K1919100
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1919100/annotation2501464/range2501464/1

https://synbiohub.org/public/igem/BBa_K1919100/annotation2501464/range2501464/1
type: Range
persistent identity: range2501464
display id: range2501464
version: 1
direction: direction/reverse
top level: BBa_K1919100
start: 1
end: 948
orientation: inline

BBa_K1497008
type: SequenceAnnotation
persistent identity: annotation2501707
display id: annotation2501707
version: 1
top level: BBa_K1919100
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1919100/annotation2501707/range2501707/1

https://synbiohub.org/public/igem/BBa_K1919100/annotation2501707/range2501707/1
type: Range
persistent identity: range2501707
display id: range2501707
version: 1
direction: direction/forward
top level: BBa_K1919100
start: 956
end: 1112
orientation: inline

BBa_K112006
type: ComponentDefinition
persistent identity: BBa K112006
display id: BBa_K112006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: T4 antiholin! in BBb
creator: Bing Xia
created: 2008-09-02T11:00:00Z
modified: 2015-05-08T01:09:13Z
discontinued: false
dominant: false
experience: experience/None
group u list: _224_
m user id: 0
owner id: 2998
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: T4 antiholin gene with start and stop codons, in BBb format. ---- This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at: [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
mutable notes: N/A
mutable provenance: PCR bx7/bx8 on Bjh1303 (325 bp, EcoRI/BamHI/DpnI) Sub into pBca1256 (EcoRI/BamHI) Product is pBca1256-K112006 ---------------------------------------- bx7 Forward biobricking of {T4 Antiholin!} ccagtGAATTCatgAGATCTatggccttaaaagcaacagcac bx8 Reverse biobricking of {T4 Antiholin!} cgttaGGATCCtcattcagtctccaatttaatgttc
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K112006_sequence/1

BBa_K112002
type: ComponentDefinition
persistent identity: BBa K112002
display id: BBa_K112002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: T4 holin, without stop codon, berkeley standard
creator: Bing Xia
created: 2008-09-02T11:00:00Z
modified: 2015-05-08T01:09:13Z
discontinued: false
dominant: false
experience: experience/None
group u list: _224_
m user id: 0
owner id: 2998
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: T4 holin gene with a start codon, without a stop codon, in BBb format. ---- This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at: [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K112002_sequence/1

BBa_K112201
type: ComponentDefinition
persistent identity: BBa K112201
display id: BBa_K112201
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: < T4 holin! in BBb
creator: Bing Xia
created: 2008-09-02T11:00:00Z
modified: 2015-05-08T01:09:14Z
discontinued: false
dominant: false
experience: experience/None
group u list: _224_
m user id: 0
owner id: 2998
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: T4 holin gene without a start codon with a stop codon, in BBb format. ---- This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at: [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence: https://synbiohub.org/public/igem/BBa_K112201_sequence/1

BBa_K112312
type: ComponentDefinition
persistent identity: BBa K112312
display id: BBa_K112312
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: {lambda antiholin!}; adheres to Berkeley standard
creator: Aron Lau
created: 2008-10-20T11:00:00Z
modified: 2015-05-08T01:09:16Z
discontinued: false
dominant: false
experience: experience/None
group u list: _224_
m user id: 0
owner id: 2999
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: lambda antiholin with start and stop codons. This part is actually in BBb format. ---- This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at: [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
mutable notes: PCR al0013/al0008 on pSB3C6-Bjh1503 (355bp, EcoRI/BamHI/DpnI) Sub into pBca1256 (EcoRI/BamHI, 2472+910, L) Product is pBca1256-K112312 ----------------------------------------------- al0013 Forward Biobricking of lambda antiholin with start and stop codons CCATAGAATTCatgAGATCTatgaagCtgccagaaaaacatgacc al0008 Reverse Biobricking of lambda antiholin with start and stop codons CGttaGGATCCttattgatttctaccatcttctactccggc
mutable provenance: pSB3C6-Bjh1503
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K112312_sequence/1

BBa_K112307
type: ComponentDefinition
persistent identity: BBa K112307
display id: BBa_K112307
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: {< lambda holin!} in BBb format
creator: Aron Lau
created: 2008-10-20T11:00:00Z
modified: 2015-05-08T01:09:16Z
discontinued: false
dominant: false
experience: experience/None
group u list: _224_
m user id: 0
owner id: 2999
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: lambda holin with stop codon and no start codon in BBb format More information about the BBb Format is available at: [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
mutable notes: PCR al0009/al0008 on pSB3C6-Bjh1502 (346bp, EcoRI/BamHI/DpnI) Sub into pBca1256 (EcoRI/BamHI, 2472+910, L) Product is pBca1256-K112307 ----------------------------------------------- al0009 Forward Biobricking of lambda holin with stop codon and no start codon CCATAGAATTCatgAGATCTccagaaaaacatgacctgttggcc al0008 Reverse Biobricking of lambda holin with stop codon and no start codon CGttaGGATCCttattgatttctaccatcttctactccggc
mutable provenance: pSB3C6-Bjh1502
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence: https://synbiohub.org/public/igem/BBa_K112307_sequence/1

BBa_K1479016
type: ComponentDefinition
persistent identity: BBa K1479016
display id: BBa_K1479016
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
created: 2014-10-06T11:00:00Z
modified: 2015-05-08T01:10:40Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1859_
m user id: 0
owner id: 21516
owning group id: 9
sample status: No part sequence
status: status/Planning
bookmark: false
mutable description: fsadfasdfasdfasfdasdfa
mutable notes: sadfasdfadfsdfa
mutable provenance: adsfsdfasdfasdf
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Promoter
sequence annotation: 4
sequence annotation: 1

4
type: SequenceAnnotation
persistent identity: annotation2418317
display id: annotation2418317
version: 1
top level: BBa_K1479016
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1479016/annotation2418317/range2418317/1

https://synbiohub.org/public/igem/BBa_K1479016/annotation2418317/range2418317/1
type: Range
persistent identity: range2418317
display id: range2418317
version: 1
direction: direction/forward
top level: BBa_K1479016
start: 5
end: 6
orientation: inline

1
type: SequenceAnnotation
persistent identity: annotation2418316
display id: annotation2418316
version: 1
top level: BBa_K1479016
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1479016/annotation2418316/range2418316/1

https://synbiohub.org/public/igem/BBa_K1479016/annotation2418316/range2418316/1
type: Range
persistent identity: range2418316
display id: range2418316
version: 1
direction: direction/forward
top level: BBa_K1479016
start: 2
end: 3
orientation: inline

BBa_K112315
type: ComponentDefinition
persistent identity: BBa K112315
display id: BBa_K112315
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: {< lambda antiholin >}; adheres to Berkeley standard
creator: Aron Lau
created: 2008-10-20T11:00:00Z
modified: 2015-05-08T01:09:16Z
discontinued: false
dominant: false
experience: experience/None
group u list: _224_
m user id: 0
owner id: 2999
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: lambda antiholin with no start and stop codons. This part is actually in BBb format. ---- This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at: [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
mutable notes: PCR al0014/al0010 on pSB3C6-Bjh1503 (349bp, EcoRI/BamHI/DpnI) Sub into pBca1256 (EcoRI/BamHI, 2472+910, L) Product is pBca1256-K112315 ----------------------------------------------- al0014 Forward Biobricking of lambda antiholin with no start and stop codons CCATAGAATTCatgAGATCTaagCtgccagaaaaacatgacctg al0010 Reverse Biobricking of lambda antiholin with no start and stop codons CGttaGGATCCttgatttctaccatcttctactccggc
mutable provenance: pSB3C6-Bjh1503
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence: https://synbiohub.org/public/igem/BBa_K112315_sequence/1

BBa_K112008
type: ComponentDefinition
persistent identity: BBa K112008
display id: BBa_K112008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: T4 antiholin> in BBb
creator: Bing Xia
created: 2008-09-02T11:00:00Z
modified: 2015-05-08T01:09:13Z
discontinued: false
dominant: false
experience: experience/None
group u list: _224_
m user id: 0
owner id: 2998
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: T4 antiholin gene with a start codon, without a stop codon, in BBb format. ---- This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at: [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
mutable notes: N/A
mutable provenance: PCR bx7/bx10 on Bjh1303 (322 bp, EcoRI/BamHI/DpnI) Sub into pBca1256 (EcoRI/BamHI) Product is pBca1256-K112008 ---------------------------------------- bx7 Forward biobricking of {T4 Antiholin>} ccagtGAATTCatgAGATCTatggccttaaaagcaacagcac bx10 Reverse biobricking of {T4 Antiholin>} cgttaGGATCCttcagtctccaatttaatgttc
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence: https://synbiohub.org/public/igem/BBa_K112008_sequence/1

gpS
type: ComponentDefinition
persistent identity: BBa K836004
display id: BBa_K836004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Lysis inhibitor from Enterobacteria phage lambda (codon usage optimized for R. opacus)
creator: Ricardo Alvarado
created: 2012-09-19T11:00:00Z
modified: 2015-05-08T01:13:32Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1095_
m user id: 0
owner id: 11819
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This protein is the one in charge to avoid the formation of pores in the inner membrane of the bacterial cell. It has been optimized for expression in R. opacus. A weak RBS for Gram positive bacteria was used (http://partsregistry.org/Part:BBa_K090506:Design)in order to obtain low concentration of the protein.
mutable notes: We wanted a low concentration of this protein since medium to large concentrations could affect or completely inhibit the lysis device. Basal but constitutive expression of this protein is needed to avoid the formation of pores in the design lysis device.
mutable provenance: The amino sequence was obtained from Uniprot (http://www.uniprot.org/uniprot/P03705#section_seq lysis inhibitor isoform). The codon optimization for R. opacus (avoiding standard restriction sites EcoRI, PstI, XbaI and SpeI) was made using the free software GeneDesigner from DNA 2.0.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
sequence annotation: Lambda antiholin
sequence annotation: BBa_K090506
sequence annotation: Scar
sequence: https://synbiohub.org/public/igem/BBa_K836004_sequence/1

Lambda antiholin
type: SequenceAnnotation
persistent identity: annotation2187517
display id: annotation2187517
version: 1
top level: gpS
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K836004/annotation2187517/range2187517/1

https://synbiohub.org/public/igem/BBa_K836004/annotation2187517/range2187517/1
type: Range
persistent identity: range2187517
display id: range2187517
version: 1
direction: direction/forward
top level: gpS
start: 18
end: 338
orientation: inline

BBa_K090506
type: SequenceAnnotation
persistent identity: annotation2187515
display id: annotation2187515
version: 1
top level: gpS
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K836004/annotation2187515/range2187515/1

https://synbiohub.org/public/igem/BBa_K836004/annotation2187515/range2187515/1
type: Range
persistent identity: range2187515
display id: range2187515
version: 1
direction: direction/forward
top level: gpS
start: 1
end: 11
orientation: inline

Scar
type: SequenceAnnotation
persistent identity: annotation2187516
display id: annotation2187516
version: 1
top level: gpS
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K836004/annotation2187516/range2187516/1

https://synbiohub.org/public/igem/BBa_K836004/annotation2187516/range2187516/1
type: Range
persistent identity: range2187516
display id: range2187516
version: 1
direction: direction/forward
top level: gpS
start: 12
end: 17
orientation: inline

BBa_K608352
type: ComponentDefinition
persistent identity: BBa K608352
display id: BBa_K608352
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Bacteriophage Lysis Cassette with RBS
creator: Theodhor Tiko
created: 2011-09-13T11:00:00Z
modified: 2015-05-08T01:12:52Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _780_
m user id: 0
owner id: 9113
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: RBS + Lysis Genes
mutable notes: sdf
mutable provenance: sdf
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
sequence annotation: BBa_B0034
sequence annotation: R Protein (Endolysin: Phage lambda lysozyme)
sequence annotation: S' Protein (Holin) / S Protein (Anti-holin)
sequence annotation: Rz1 Protein
sequence annotation: SpeI, XbaI scar
sequence annotation: Rz Protein
sequence: https://synbiohub.org/public/igem/BBa_K608352_sequence/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2131072
display id: annotation2131072
version: 1
top level: BBa_K608352
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K608352/annotation2131072/range2131072/1

https://synbiohub.org/public/igem/BBa_K608352/annotation2131072/range2131072/1
type: Range
persistent identity: range2131072
display id: range2131072
version: 1
direction: direction/forward
top level: BBa_K608352
start: 1
end: 12
orientation: inline

R Protein (Endolysin: Phage lambda lysozyme)
type: SequenceAnnotation
persistent identity: annotation2131075
display id: annotation2131075
version: 1
top level: BBa_K608352
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K608352/annotation2131075/range2131075/1

https://synbiohub.org/public/igem/BBa_K608352/annotation2131075/range2131075/1
type: Range
persistent identity: range2131075
display id: range2131075
version: 1
direction: direction/forward
top level: BBa_K608352
start: 322
end: 798
orientation: inline

S' Protein (Holin) / S Protein (Anti-holin)
type: SequenceAnnotation
persistent identity: annotation2131074
display id: annotation2131074
version: 1
top level: BBa_K608352
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K608352/annotation2131074/range2131074/1

https://synbiohub.org/public/igem/BBa_K608352/annotation2131074/range2131074/1
type: Range
persistent identity: range2131074
display id: range2131074
version: 1
direction: direction/forward
top level: BBa_K608352
start: 21
end: 338
orientation: inline

Rz1 Protein
type: SequenceAnnotation
persistent identity: annotation2131764
display id: annotation2131764
version: 1
top level: BBa_K608352
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K608352/annotation2131764/range2131764/1

https://synbiohub.org/public/igem/BBa_K608352/annotation2131764/range2131764/1
type: Range
persistent identity: range2131764
display id: range2131764
version: 1
direction: direction/forward
top level: BBa_K608352
start: 1015
end: 1197
orientation: inline

SpeI, XbaI scar
type: SequenceAnnotation
persistent identity: annotation2131073
display id: annotation2131073
version: 1
top level: BBa_K608352
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K608352/annotation2131073/range2131073/1

https://synbiohub.org/public/igem/BBa_K608352/annotation2131073/range2131073/1
type: Range
persistent identity: range2131073
display id: range2131073
version: 1
direction: direction/forward
top level: BBa_K608352
start: 13
end: 20
orientation: inline

Rz Protein
type: SequenceAnnotation
persistent identity: annotation2131076
display id: annotation2131076
version: 1
top level: BBa_K608352
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K608352/annotation2131076/range2131076/1

https://synbiohub.org/public/igem/BBa_K608352/annotation2131076/range2131076/1
type: Range
persistent identity: range2131076
display id: range2131076
version: 1
direction: direction/forward
top level: BBa_K608352
start: 795
end: 1256
orientation: inline

gpS
type: ComponentDefinition
persistent identity: BBa K836006
display id: BBa_K836006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Lysis protein S from Enterobacteria phage lambda (codon usage optimized for R. opacus)
creator: Ricardo Alvarado
created: 2012-09-19T11:00:00Z
modified: 2015-05-08T01:13:32Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1095_
m user id: 0
owner id: 11819
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This gene codifies for a protein that makes holes in the inner membrane of the bacterial cell. A Gram positive RBS (SpoVG, http://partsregistry.org/wiki/index.php?title=Part:BBa_K143021) was added at the beginning.
mutable notes: Between the RBS and the CDS a spacer consisting on a scar from a standard suffix and a preffix for CDS was inserted.
mutable provenance: The amino sequence was obtained from Uniprot (http://www.uniprot.org/uniprot/P03705#section_seq lysis protein S) and optimized for R. opacus (avoiding standard restriction sites) using the free software GeneDesigner from DNA 2.0.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
sequence annotation: SpoVG
sequence annotation: Holin
sequence annotation: Spacer
sequence: https://synbiohub.org/public/igem/BBa_K836006_sequence/1

SpoVG
type: SequenceAnnotation
persistent identity: annotation2187525
display id: annotation2187525
version: 1
top level: gpS
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K836006/annotation2187525/range2187525/1

https://synbiohub.org/public/igem/BBa_K836006/annotation2187525/range2187525/1
type: Range
persistent identity: range2187525
display id: range2187525
version: 1
direction: direction/forward
top level: gpS
start: 1
end: 12
orientation: inline

Holin
type: SequenceAnnotation
persistent identity: annotation2187528
display id: annotation2187528
version: 1
top level: gpS
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K836006/annotation2187528/range2187528/1

https://synbiohub.org/public/igem/BBa_K836006/annotation2187528/range2187528/1
type: Range
persistent identity: range2187528
display id: range2187528
version: 1
direction: direction/forward
top level: gpS
start: 19
end: 333
orientation: inline

Spacer
type: SequenceAnnotation
persistent identity: annotation2187527
display id: annotation2187527
version: 1
top level: gpS
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K836006/annotation2187527/range2187527/1

https://synbiohub.org/public/igem/BBa_K836006/annotation2187527/range2187527/1
type: Range
persistent identity: range2187527
display id: range2187527
version: 1
direction: direction/forward
top level: gpS
start: 13
end: 18
orientation: inline

BBa_K112309
type: ComponentDefinition
persistent identity: BBa K112309
display id: BBa_K112309
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: {< lambda holin >}; adheres to Berkley standard
creator: Aron Lau
created: 2008-10-20T11:00:00Z
modified: 2015-05-08T01:09:16Z
discontinued: false
dominant: false
experience: experience/None
group u list: _224_
m user id: 0
owner id: 2999
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: lambda holin with no start and stop codon in BBb format. More information about the BBb Format is available at: [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
mutable notes: PCR al0009/al0010 on pSB3C6-Bjh1502 (343bp, EcoRI/BamHI/DpnI) Sub into pBca1256 (EcoRI/BamHI, 2472+910, L) Product is pBca1256-K112309 ----------------------------------------------- al0009 Forward Biobricking of lambda holin with no start and stop codon CCATAGAATTCatgAGATCTccagaaaaacatgacctgttggcc al0010 Reverse Biobricking of lambda holin with no start and stop codon CGttaGGATCCttgatttctaccatcttctactccggc
mutable provenance: pSB3C6-Bjh1502
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence: https://synbiohub.org/public/igem/BBa_K112309_sequence/1

BBa_K358004
type: ComponentDefinition
persistent identity: BBa K358004
display id: BBa_K358004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: lambda lysis cassette[SRRz/Rz1]
creator: Ken Kajita
created: 2010-09-18T11:00:00Z
modified: 2015-05-08T01:12:12Z
discontinued: false
dominant: false
group u list: _455_
m user id: 0
owner id: 7170
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This part causes cell death. It contains holin/antiholin, endolysin and Rz/Rz1 genes.
mutable notes: We made point mutation and prepared wild type phage DNA.
mutable provenance: λ -Hind Ⅲ digest (product of TaKaRa Bio)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
sequence annotation: holin
sequence annotation: Rz1
sequence annotation: endolysin
sequence annotation: Rz
sequence annotation: wt-RBS
sequence annotation: antiholin
sequence: https://synbiohub.org/public/igem/BBa_K358004_sequence/1

holin
type: SequenceAnnotation
persistent identity: annotation2080448
display id: annotation2080448
version: 1
top level: BBa_K358004
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K358004/annotation2080448/range2080448/1

https://synbiohub.org/public/igem/BBa_K358004/annotation2080448/range2080448/1
type: Range
persistent identity: range2080448
display id: range2080448
version: 1
direction: direction/forward
top level: BBa_K358004
start: 35
end: 352
orientation: inline

Rz1
type: SequenceAnnotation
persistent identity: annotation2080451
display id: annotation2080451
version: 1
top level: BBa_K358004
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K358004/annotation2080451/range2080451/1

https://synbiohub.org/public/igem/BBa_K358004/annotation2080451/range2080451/1
type: Range
persistent identity: range2080451
display id: range2080451
version: 1
direction: direction/forward
top level: BBa_K358004
start: 1029
end: 1211
orientation: inline

endolysin
type: SequenceAnnotation
persistent identity: annotation2080449
display id: annotation2080449
version: 1
top level: BBa_K358004
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K358004/annotation2080449/range2080449/1

https://synbiohub.org/public/igem/BBa_K358004/annotation2080449/range2080449/1
type: Range
persistent identity: range2080449
display id: range2080449
version: 1
direction: direction/forward
top level: BBa_K358004
start: 336
end: 812
orientation: inline

Rz
type: SequenceAnnotation
persistent identity: annotation2080450
display id: annotation2080450
version: 1
top level: BBa_K358004
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K358004/annotation2080450/range2080450/1

https://synbiohub.org/public/igem/BBa_K358004/annotation2080450/range2080450/1
type: Range
persistent identity: range2080450
display id: range2080450
version: 1
direction: direction/forward
top level: BBa_K358004
start: 809
end: 1270
orientation: inline

wt-RBS
type: SequenceAnnotation
persistent identity: annotation2080446
display id: annotation2080446
version: 1
top level: BBa_K358004
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K358004/annotation2080446/range2080446/1

https://synbiohub.org/public/igem/BBa_K358004/annotation2080446/range2080446/1
type: Range
persistent identity: range2080446
display id: range2080446
version: 1
direction: direction/forward
top level: BBa_K358004
start: 2
end: 26
orientation: inline

antiholin
type: SequenceAnnotation
persistent identity: annotation2080447
display id: annotation2080447
version: 1
top level: BBa_K358004
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K358004/annotation2080447/range2080447/1

https://synbiohub.org/public/igem/BBa_K358004/annotation2080447/range2080447/1
type: Range
persistent identity: range2080447
display id: range2080447
version: 1
direction: direction/forward
top level: BBa_K358004
start: 29
end: 352
orientation: inline

BBa_K112004
type: ComponentDefinition
persistent identity: BBa K112004
display id: BBa_K112004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: a~T4 holin! in BBb
creator: Bing Xia
created: 2008-09-02T11:00:00Z
modified: 2015-05-08T01:09:13Z
discontinued: false
dominant: false
experience: experience/None
group u list: _224_
m user id: 0
owner id: 2998
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: T4 holin gene ready to have an rbs attached with a stop codon, in BBb format. ---- This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at: [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
mutable notes: N/A
mutable provenance: PCR bx5/bx2 on Bjh1301 (692 bp, EcoRI/BamHI/DpnI) Sub into pBca1256 (EcoRI/BamHI) Product is pBca1256-K112004 ---------------------------------------- bx5 Forward Biobricking of {a~T4 Holin!} ccagtGAATTCatgAGATCTgtctatggcagcacctagaatatc bx2 Reverse Biobricking of {a~T4 Holin!} cgttaGGATCCttatttagcccttcctaatattctggccgcc
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K112004_sequence/1

BBa_K124014
type: ComponentDefinition
persistent identity: BBa K124014
display id: BBa_K124014
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Bacteriophage Holin Gene pS105
creator: Katherine Jacobs
created: 2008-10-08T11:00:00Z
modified: 2015-05-08T01:09:44Z
discontinued: false
dominant: false
experience: experience/None
group u list: _221_
m user id: 0
owner id: 3367
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: The Cell Lysis Cassette consists of an S, R, and Rz protein. When paired with an activator, the cassette is expressed and cell lysis is induced. The S105 strain of the cassette has the initial 6 base pairs at the beginning of the wild type sequence removed. This causes cell lysis to be induced at a faster rate than the wild type.
mutable notes: No design considerations.
mutable provenance: This part was graciously donated to the Brown iGEM Team by Ry Young at Texas A&M.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K124014_sequence/1

BBa_K112310
type: ComponentDefinition
persistent identity: BBa K112310
display id: BBa_K112310
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: {a~lambda holin!}; adheres to Berkeley standard
creator: Aron Lau
created: 2008-10-20T11:00:00Z
modified: 2015-05-08T01:09:16Z
discontinued: false
dominant: false
experience: experience/None
group u list: _224_
m user id: 0
owner id: 2999
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: lambda holin ready for rbs addition and has stop codon in BBb format. More information about the BBb Format is available at: [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
mutable notes: PCR al0011/al0008 on pSB3C6-Bjh1502 (353bp, EcoRI/BamHI/DpnI) Sub into pBca1256 (EcoRI/BamHI, 2472+910, L) Product is pBca1256-K112310 ----------------------------------------------- al0011 Forward Biobricking of lambda holin with spacer CCATAGAATTCatgAGATCTgaagatgccagaaaaacatgacctg al0008 Reverse Biobricking of lambda holin with spacer CGttaGGATCCttattgatttctaccatcttctactccggc
mutable provenance: pSB3C6-Bjh1502
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K112310_sequence/1

BBa_K112009
type: ComponentDefinition
persistent identity: BBa K112009
display id: BBa_K112009
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: <T4 antiholin> in BBb
creator: Bing Xia
created: 2008-09-02T11:00:00Z
modified: 2015-05-08T01:09:13Z
discontinued: false
dominant: false
experience: experience/None
group u list: _224_
m user id: 0
owner id: 2998
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: T4 antiholin gene without start and stop codons, in BBb format. ---- This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at: [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
mutable notes: N/A
mutable provenance: PCR bx9/bx10 on Bjh1303 (319 bp, EcoRI/BamHI/DpnI) Sub into pBca1256 (EcoRI/BamHI) Product is pBca1256-K112009 ---------------------------------------- bx9 Forward biobricking of {<T4 Antiholin>} ccagtGAATTCatgAGATCTgccttaaaagcaacagcac bx10 Reverse biobricking of {<T4 Antiholin>} cgttaGGATCCttcagtctccaatttaatgttc
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K112009_sequence/1

BBa_K1639006
type: ComponentDefinition
persistent identity: BBa K1639006
display id: BBa_K1639006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PotB59-pomA
creator: Mustafa Yılmaz
created: 2015-09-11T11:00:00Z
modified: 2015-09-21T05:08:59Z
discontinued: false
dominant: false
group u list: _2056_
m user id: 24789
owner id: 20835
owning group id: 9
status: status/Planning
bookmark: false
mutable description: PotB59, chimeric protein that is formed by the N-Terminal region of PomB and the C-terminal of E. coli MotB It was shown that a chimeric protein, PotB, renamed as PotB59, that has the N-terminal region of PomB (1???50) fused to the C-terminal periplasmic region of E. coli MotB (59???308), is functional with PomA in E. coli as well as in strains of V. alginolyticus that are defective in motX or motY (Asai et al. 2003) Instead of the hydrogen-dependant MotA MotB Stator protein partially adapted from the V. alginolyticus, sodium-dependant Stator proteins were installed. After this adaptation, the flagellar velocity increased by twice fold.
mutable notes: This part contains an additional BamHI restriction site at beginning of TlpB sequence and a XhoI restriction site at the end.
mutable provenance: asd
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: PotB59
sequence annotation: PomA
sequence annotation: Ribosome Reinitiation Spacer
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_K1639006_sequence/1

PotB59
type: SequenceAnnotation
persistent identity: annotation2452964
display id: annotation2452964
version: 1
top level: BBa_K1639006
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1639006/annotation2452964/range2452964/1

https://synbiohub.org/public/igem/BBa_K1639006/annotation2452964/range2452964/1
type: Range
persistent identity: range2452964
display id: range2452964
version: 1
direction: direction/forward
top level: BBa_K1639006
start: 9
end: 911
orientation: inline

PomA
type: SequenceAnnotation
persistent identity: annotation2452965
display id: annotation2452965
version: 1
top level: BBa_K1639006
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1639006/annotation2452965/range2452965/1

https://synbiohub.org/public/igem/BBa_K1639006/annotation2452965/range2452965/1
type: Range
persistent identity: range2452965
display id: range2452965
version: 1
direction: direction/forward
top level: BBa_K1639006
start: 965
end: 1723
orientation: inline

Ribosome Reinitiation Spacer
type: SequenceAnnotation
persistent identity: annotation2452966
display id: annotation2452966
version: 1
top level: BBa_K1639006
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1639006/annotation2452966/range2452966/1

https://synbiohub.org/public/igem/BBa_K1639006/annotation2452966/range2452966/1
type: Range
persistent identity: range2452966
display id: range2452966
version: 1
direction: direction/forward
top level: BBa_K1639006
start: 915
end: 935
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2452967
display id: annotation2452967
version: 1
top level: BBa_K1639006
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1639006/annotation2452967/range2452967/1

https://synbiohub.org/public/igem/BBa_K1639006/annotation2452967/range2452967/1
type: Range
persistent identity: range2452967
display id: range2452967
version: 1
direction: direction/forward
top level: BBa_K1639006
start: 936
end: 958
orientation: inline

BBa_K112010
type: ComponentDefinition
persistent identity: BBa K112010
display id: BBa_K112010
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: a~T4 antiholin! in BBb
creator: Bing Xia
created: 2008-09-02T11:00:00Z
modified: 2015-05-08T01:09:13Z
discontinued: false
dominant: false
experience: experience/None
group u list: _224_
m user id: 0
owner id: 2998
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: T4 antiholin gene ready to have an rbs attached to the front, with a stop codon, in BBb format. ---- This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at: [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
mutable notes: N/A
mutable provenance: PCR bx11/bx8 on Bjh1303 (329 bp, EcoRI/BamHI/DpnI) Sub into pBca1256 (EcoRI/BamHI) Product is pBca1256-K112010 ---------------------------------------- bx11 Forward biobricking of {a~T4 Antiholin!} ccagtGAATTCatgAGATCTctttatggccttaaaagcaacagc bx8 Reverse biobricking of {a~T4 Antiholin!} cgttaGGATCCtcattcagtctccaatttaatgttc
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K112010_sequence/1

BBa_K112308
type: ComponentDefinition
persistent identity: BBa K112308
display id: BBa_K112308
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: {lambda holin >}; adheres to Berkeley standard
creator: Aron Lau
created: 2008-10-20T11:00:00Z
modified: 2015-05-08T01:09:16Z
discontinued: false
dominant: false
experience: experience/None
group u list: _224_
m user id: 0
owner id: 2999
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: lambda holin with start and no stop codon in BBb format. More information about the BBb Format is available at: [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
mutable notes: PCR al0007/al0010 on pSB3C6-Bjh1502 (346bp, EcoRI/BamHI/DpnI) Sub into pBca1256 (EcoRI/BamHI, 2472+910, L) Product is pBca1256-K112308 ----------------------------------------------- al0007 Forward Biobricking of lambda holin with start and no stop codon CCATAGAATTCatgAGATCTatgccagaaaaacatgacctgttgg al0010 Reverse Biobricking of lambda holin wtih start and no stop codon CGttaGGATCCttgatttctaccatcttctactccggc
mutable provenance: pSB3C6-Bjh1502
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence: https://synbiohub.org/public/igem/BBa_K112308_sequence/1

BBa_K112000
type: ComponentDefinition
persistent identity: BBa K112000
display id: BBa_K112000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: T4 holin, complete CDS, berkeley standard
creator: Bing Xia
created: 2008-09-02T11:00:00Z
modified: 2015-05-08T01:09:13Z
discontinued: false
dominant: false
experience: experience/None
group u list: _224_
m user id: 0
owner id: 2998
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: T4 holin gene, with start and stop codons. This part is actually in BBb format.
mutable provenance: PCR from existing part.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K112000_sequence/1

BBa_K112313
type: ComponentDefinition
persistent identity: BBa K112313
display id: BBa_K112313
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: {< lambda antiholin!}; adheres to Berkeley standard
creator: Aron Lau
created: 2008-10-20T11:00:00Z
modified: 2015-05-08T01:09:16Z
discontinued: false
dominant: false
experience: experience/None
group u list: _224_
m user id: 0
owner id: 2999
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: lambda antiholin with stop codon and no start codon. This part is actually in BBb format. ---- This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at: [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
mutable notes: PCR al0014/al0008 on pSB3C6-Bjh1503 (352bp, EcoRI/BamHI/DpnI) Sub into pBca1256 (EcoRI/BamHI, 2472+910, L) Product is pBca1256-K112313 ----------------------------------------------- al0014 Forward Biobricking of lambda antiholin with stop codon and no start codon CCATAGAATTCatgAGATCTaagCtgccagaaaaacatgacctg al0008 Reverse Biobricking of lambda antiholin with stop codon and no start codon CGttaGGATCCttattgatttctaccatcttctactccggc
mutable provenance: pSB3C6-Bjh1503
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence: https://synbiohub.org/public/igem/BBa_K112313_sequence/1

BBa_K112316
type: ComponentDefinition
persistent identity: BBa K112316
display id: BBa_K112316
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: {a~lambda antiholin!}; adheres to Berkeley standard
creator: Aron Lau
created: 2008-10-20T11:00:00Z
modified: 2015-05-08T01:09:16Z
discontinued: false
dominant: false
experience: experience/None
group u list: _224_
m user id: 0
owner id: 2999
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: lambda antiholin ready for rbs addition and has stop codon. This part is actually in BBb format. ---- This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at: [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
mutable notes: PCR al0015/al0008 on pSB3C6-Bjh1503 (359bp, EcoRI/BamHI/DpnI) Sub into pBca1256 (EcoRI/BamHI, 2472+910, L) Product is pBca1256-K112316 ----------------------------------------------- al0015 Forward Biobricking of lambda antiholin with spacer CCATAGAATTCatgAGATCTagacatgaagCtgccagaaaaacatgacc al0008 Reverse Biobricking of lambda antiholin with spacer CGttaGGATCCttattgatttctaccatcttctactccggc
mutable provenance: pSB3C6-Bjh1503
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K112316_sequence/1

BBa_K112007
type: ComponentDefinition
persistent identity: BBa K112007
display id: BBa_K112007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: < T4 antiholin! in BBb
creator: Bing Xia
created: 2008-09-02T11:00:00Z
modified: 2015-05-08T01:09:13Z
discontinued: false
dominant: false
experience: experience/None
group u list: _224_
m user id: 0
owner id: 2998
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: T4 antiholin gene with a start codon, with a stop codon, in BBb format. ---- This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at: [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
mutable notes: N/A
mutable provenance: PCR bx9/bx8 on Bjh1303 (322 bp, EcoRI/BamHI/DpnI) Sub into pBca1256 (EcoRI/BamHI) Product is pBca1256-K112007 ---------------------------------------- bx9 Forward biobricking of {<T4 Antiholin!} ccagtGAATTCatgAGATCTgccttaaaagcaacagcac bx8 Reverse biobricking of {<T4 Antiholin!} cgttaGGATCCtcattcagtctccaatttaatgttc
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence: https://synbiohub.org/public/igem/BBa_K112007_sequence/1

BBa_K112306
type: ComponentDefinition
persistent identity: BBa K112306
display id: BBa_K112306
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: {lambda holin!} in BBb format
creator: Aron Lau
created: 2008-10-20T11:00:00Z
modified: 2015-05-08T01:09:16Z
discontinued: false
dominant: true
experience: experience/None
group u list: _224_
m user id: 0
owner id: 2999
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: lambda holin with start and stop codons in BBb format. More information about the BBb Format is available at: [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
mutable notes: PCR al0007/al0008 on pSB3C6-Bjh1502 (349bp, EcoRI/BamHI/DpnI) Sub into pBca1256 (EcoRI/BamHI, 2472+910, L) Product is pBca1256-K112306 ----------------------------------------------- al0007 Forward Biobricking of lambda holin with start and stop codons CCATAGAATTCatgAGATCTatgccagaaaaacatgacctgttgg al0008 Reverse Biobricking of lambda holin with start and stop codons CGttaGGATCCttattgatttctaccatcttctactccggc
mutable provenance: pSB3C6-Bjh1502
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K112306_sequence/1

BBa_K112314
type: ComponentDefinition
persistent identity: BBa K112314
display id: BBa_K112314
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: { lambda antiholin >}; adheres to Berkeley standard
creator: Aron Lau
created: 2008-10-20T11:00:00Z
modified: 2015-05-08T01:09:16Z
discontinued: false
dominant: false
experience: experience/None
group u list: _224_
m user id: 0
owner id: 2999
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: lambda antiholin with start codon and no stop codon. This part is actually in BBb format. ---- This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at: [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
mutable notes: PCR al0013/al0010 on pSB3C6-Bjh1503 (352bp, EcoRI/BamHI/DpnI) Sub into pBca1256 (EcoRI/BamHI, 2472+910, L) Product is pBca1256-K112314 ----------------------------------------------- al0013 Forward Biobricking of lambda antiholin with start codon and no stop codon CCATAGAATTCatgAGATCTatgaagCtgccagaaaaacatgacc al0010 Reverse Biobricking of lambda antiholin with start codon and no stop codon CGttaGGATCCttgatttctaccatcttctactccggc
mutable provenance: pSB3C6-Bjh1503
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence: https://synbiohub.org/public/igem/BBa_K112314_sequence/1