GlpF is an aquaglyceroporin channel that facilitates the transport of As(III).
This part comes from genomic DNA of the Escherichia coli 536.
E.coli K-12 coding region for FecA protein
Ptac-NiCoTB, improves nickel and cobalt internalization
This part comprises a strong E. coli promoter with...
Ptac-NiCoTB-rcn-csgBAEFG, turns E. coli into a functional Ni and Co biofilter
This part, when expressed in a rcnA E. coli strain, allows the formation of a functional Ni2+ and Co2+ biofilter capturing up to 3 mg of metal per g of bacterial dry weight when exposed to 20 ??M Ni2+ or Co2+, in only 10 mn. This is one of the best performances and the fastest reported until now.
[lien] and [lien] were cloned by 3A assembly.
mtrCAB - gene cluster from S. oneidensis for cytochrome expression
Minimal set of genes for the expression of working cytochromes. Allows the organism to transfer electrons to its outer membrane.
Two illegal PstI restriction sites, which had to be removed by specific primer extensions.
This part was derived from the Pbr T lead uptake protein from Ralstonia metallidurans. The protein is responsible for the uptake of Pb 2+ ions. This part can be used for projects where one needs to uptake lead ions from the environment into the cell interior.
When designing the part we checked the gene sequence/restriction site profile to make sure the part would be compatible with standards 10 and 23.
Borremans,B., Hobman,J.L., Provoost,A., Brown,N.L. and van Der Lelie,D. "Cloning and functional analysis of the pbr lead resistance determinant of Ralstonia metallidurans CH34" J. Bacteriol. 183 (19), 5651-5658 (2001)
Export of recombinant proteins in Escherichia coli using ABC transporter of Erwinia chrysanthemi
Jung Hoon Ahn from Korea Advanced Institute of Science and Technology
EGF-TliA, EGF-LARD1 fusion proteins were secreted when ABC transporter was present. E. coli harboring the PrtDEF of E. chrysanthemi secreted more fusion proteins than without ABC transporter. In fact, in enzyme activation assay(with Hitachi Spectrophotometer) we observed that although our protein -EGF- has disulfide bonds, it was excreted succesfully. In addition, when compearing with lack of ABC domain, we saw that protein excretion into medium (supernatant) is 10-fold more with ABC transporter-PrtDEF-. PrtDEF functioned well at 37??C, the optimum growth temperature for E. coli
We used whole plasmid in order to excrete a fusion protein with one of two signal tags which are TliA and LARD1
Sequence and Features PrtDEF: ABC transporter of Erwinia chrysanthemi for PrtA EGF: Human Epidermal Growth Factor
A transporter which is involved in transporting atrazine.
TRM is a trans-membrane transporter which involves in transporting atrazine.
It comes from the genome of Acinetobacter baumannii.
Antigen 43 (Ag43) is the product of the flu gene and works as a cell-surface autotransporter protein found in Escherichia coli. It is expressed to induce cell-cell aggregation and is involved in biofilm formation.
We made a fusion protein of Ag43 and YFP to visualise the clumps of cells
Periplasm transport visualization device
J23119 promoter for Synechocystis 6803 + cmpA.
malE - a monomer. This monomeric protein is used as a negative control when testing for dimerization.
We used a linker attached to a protein membrane "estA"
Zymomonas mobilis glucose facilitator gene
Lipase ABC transporter recognition domain (LARD 1)
Lard 1 was designed for the secretion of fusion proteins.The LARD 1 included four glycine-rich repeats comprising a β-roll structure, and were added to the C-terminus of test proteins. Either Pro-Gly linker or Factor Xa site can be added between fusion proteins and LARD 1. Upon supplementation of E. coli with ABC transporter from E. chrysanthemi (ABC transporter PrtDEF), which function well in the phylogenetically-neighboring genus E.coli, EGF-LARDs were excreted into the culture supernatant.
In our designed LARD 1, we favoured not to add these cleavage site so if you want, yo can add either Pro-Gly linker or Factor Xa site. Secretion of fusion proteins with LARDs could be detected by Western blotting. Fusion proteins were traced in the culture supernatant using antibody against LARDs. Polyclonal antibodies against the C-terminal signal sequence were produced with a synthetic peptide (YQPTDRLVFQGADGST, residues 421???436 of TliA ,also of LARD).
LARD 1 composed of residues 303-476 of thermostable lipase (TliA) of Pseudomonas fluorescens.
trkD, a functional Kup (formerly TrkD) system took up Cs+ with a moderate rate and affinity
Escherichia coli cells which contain a functional Kup (formerly TrkD) system took up Cs' with a moderate rate and affinity. Kup is a separate K+ uptake system with relatively little discrimination in the transport of the cations K+, Rb+, and Cs'. Regardless of the presence or absence of Kup, K+-replete cells took up Cs'primarily by a very low affinity mode, proportional to the ratio of the Cs' and K+ concentrations in the medium. In our project, trkD is used to collect Cs-137 when recN is activated by radiation and start the transcription of trkD,in order to absorb this kind of radioactive source like Cs-137
-Part was synthesized to already contain RBS. -Scar between RBS and ORF is TACTAG. -The part was synthesized with with BioBrick prefix and suffix. -EcoR1 site was preceded by CCG to allow us to clone the part into pSB1C3. -
The Ucp1 is a DNA part which encodes for a protein called UCP1 which belongs to the uncuopling proteins family. The uncoupling proteins (UCPs) are transporters, presents in the mitochondrial inner membrane, that mediate a regulated discharge of the proton gradient that is generated by the respiratory chain. This energy-dissipatory mechanism can serve functions such as termogenesis. The protein is regulated by acivation and inhibition in the mitochondrion. The proton conductance in UCP1 is inhibited by nucleotides and activated by fatty acids.
Uncouplin protein UCP1 is a protein that is found in the mitochondria from brown adipocytes.