//cds/reporter/gfp
type: Collection
persistent identity: cds reporter gfp
display id: cds_reporter_gfp
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
owned by: james
owned by: myers
member: BBa_K1399016
member: BBa_K1740000
member: BBa_K1486034
member: BBa_K1680017
member: BBa_J52021
member: BBa_J52026
member: BBa_K1399023
member: BBa_K1468000
member: BBa_K1789004
member: BBa_K1680019
member: GFP
member: BBa_K1399014
member: BBa_K1486041
member: GFP_Freibu
member: BBa_K1399018
member: BBa_K1680023
member: GFPLVA
member: sfGFP(Sp)
member: BBa_K1399019
member: BBa_K1486023
member: BBa_K1980010
member: J23101::gf
member: MymTsfGFP
member: BBa_K1680024
member: BBa_K1486040
member: BBa_K1909014
member: BBa_K2023016
member: BBa_K1468002
member: BBa_K1700000
member: BBa_K1740001
member: BBa_K1824100
member: BBa_K1909013
member: BBa_K1650038
member: BBa_K294055
member: BBa_K106028
member: BBa_K1497020
member: BBa_K1980005
member: BBa_K1486005
member: BBa_J97001
member: BBa_K1321337
member: BBa_K1680020
member: HisTag-GFP
member: BBa_K1321346
member: BBa_K1399015
member: BBa_K1980012
member: BBa_K082003
member: BBa_K1399017
member: GFP_His
member: GFP-Si4Tag
member: BBa_K1467204
member: BBa_K125500
member: BBa_K1680018
member: BBa_K1732022
member: BBa_K1486028
member: BBa_K1680006
member: BBa_K1709003
member: BBa_K106671
member: pUSPA::gfp
member: BBa_K1486002
member: BBa_K1673410
member: BBa_K1732020
member: BBa_K1399022
member: BBa_K1789003
member: BBa_K1321342
member: BBa_K1321348
member: BBa_K1399021
member: BBa_K180001
member: CheZ-GFP
member: BBa_K1486038
member: BBa_K648013
member: BBa_K1399004
member: BBa_K1742003

BBa_K1399019
type: ComponentDefinition
persistent identity: BBa K1399019
display id: BBa_K1399019
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Ptet-GFP(AAV)
creator: Anna Stikane
created: 2014-09-18T11:00:00Z
modified: 2015-05-08T01:10:16Z
discontinued: false
dominant: false
group u list: _1777_
m user id: 0
owner id: 22477
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: Tetracycline/aTc inducible promoter with GFP reporter tagged with AAV-ssrA degradation tag followed by terminator. The tagged GFP is actively degraded within cell, thus provides better temporal resolution of green fluorescence and promoter activity.
mutable notes: Part was assembled using BrickClip assembly (BBF RFC104) using other biobrick parts as templates. BrickClip assembly is a special case of more general Paperclip assembly method.[1]
mutable provenance: other biobricks
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_B0034
component: BBa_B0015
component: BBa_R0040
component: BBa_K1399005
sequence annotation: BBa_R0040
sequence annotation: BBa_B0015
sequence annotation: BBa_K1399005
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K1399019_sequence/1

BBa_B0034
type: Component
persistent identity: component2384225
display id: component2384225
version: 1
top level: BBa_K1399019
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0015
type: Component
persistent identity: component2384238
display id: component2384238
version: 1
top level: BBa_K1399019
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_R0040
type: Component
persistent identity: component2384219
display id: component2384219
version: 1
top level: BBa_K1399019
access: public
definition: https://synbiohub.org/public/igem/BBa_R0040/1

BBa_K1399005
type: Component
persistent identity: component2384231
display id: component2384231
version: 1
top level: BBa_K1399019
access: public
definition: https://synbiohub.org/public/igem/BBa_K1399005/1

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation2384219
display id: annotation2384219
version: 1
top level: BBa_K1399019
location: https://synbiohub.org/public/igem/BBa_K1399019/annotation2384219/range2384219/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_K1399019/annotation2384219/range2384219/1
type: Range
persistent identity: range2384219
display id: range2384219
version: 1
direction: direction/forward
top level: BBa_K1399019
start: 1
end: 54
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2384238
display id: annotation2384238
version: 1
top level: BBa_K1399019
location: https://synbiohub.org/public/igem/BBa_K1399019/annotation2384238/range2384238/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K1399019/annotation2384238/range2384238/1
type: Range
persistent identity: range2384238
display id: range2384238
version: 1
direction: direction/reverse
top level: BBa_K1399019
start: 842
end: 970
orientation: inline

BBa_K1399005
type: SequenceAnnotation
persistent identity: annotation2384231
display id: annotation2384231
version: 1
top level: BBa_K1399019
location: https://synbiohub.org/public/igem/BBa_K1399019/annotation2384231/range2384231/1
component: BBa_K1399005

https://synbiohub.org/public/igem/BBa_K1399019/annotation2384231/range2384231/1
type: Range
persistent identity: range2384231
display id: range2384231
version: 1
direction: direction/reverse
top level: BBa_K1399019
start: 81
end: 833
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2384225
display id: annotation2384225
version: 1
top level: BBa_K1399019
location: https://synbiohub.org/public/igem/BBa_K1399019/annotation2384225/range2384225/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1399019/annotation2384225/range2384225/1
type: Range
persistent identity: range2384225
display id: range2384225
version: 1
direction: direction/reverse
top level: BBa_K1399019
start: 63
end: 74
orientation: inline

BBa_K1680021
type: ComponentDefinition
persistent identity: BBa K1680021
display id: BBa_K1680021
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Dronpa caged Cre with NLS
creator: Nicolai von Kuegelgen
created: 2015-09-16T11:00:00Z
modified: 2016-01-26T01:27:03Z
discontinued: false
dominant: false
group u list: _2098_
m user id: 4206
owner id: 26366
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Protein coding region for a fusion protein of Cre recombinase (BBa_K1680007) that is C- & N-terminal flanked with 145N Dronpa (BBa_K1680006) using a 6 AA linker as spacer (BBa_K1680000). The construct has a N-terminal NLS sequence (BBa_K1680004). In the expressed construct the two dronpa domains should form multimerize leading to an inactive Cre recombinase that is reversibly activatable through dronpa illumination (see Zhou et al 2012).
mutable notes: 3A Assembly using RFC25
mutable provenance: gBlock design by Team Tuebingen
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Dronpa
sequence annotation: linker
sequence annotation: SV40 NLS
sequence annotation: Cre recombinase
sequence: https://synbiohub.org/public/igem/BBa_K1680021_sequence/1

Dronpa
type: SequenceAnnotation
persistent identity: annotation2477374
display id: annotation2477374
version: 1
top level: BBa_K1680021
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1680021/annotation2477374/range2477374/1

https://synbiohub.org/public/igem/BBa_K1680021/annotation2477374/range2477374/1
type: Range
persistent identity: range2477374
display id: range2477374
version: 1
direction: direction/forward
top level: BBa_K1680021
start: 31
end: 699
orientation: inline

linker
type: SequenceAnnotation
persistent identity: annotation2477377
display id: annotation2477377
version: 1
top level: BBa_K1680021
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1680021/annotation2477377/range2477377/1

https://synbiohub.org/public/igem/BBa_K1680021/annotation2477377/range2477377/1
type: Range
persistent identity: range2477377
display id: range2477377
version: 1
direction: direction/forward
top level: BBa_K1680021
start: 1747
end: 1764
orientation: inline

linker
type: SequenceAnnotation
persistent identity: annotation2477375
display id: annotation2477375
version: 1
top level: BBa_K1680021
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1680021/annotation2477375/range2477375/1

https://synbiohub.org/public/igem/BBa_K1680021/annotation2477375/range2477375/1
type: Range
persistent identity: range2477375
display id: range2477375
version: 1
direction: direction/forward
top level: BBa_K1680021
start: 700
end: 717
orientation: inline

SV40 NLS
type: SequenceAnnotation
persistent identity: annotation2477373
display id: annotation2477373
version: 1
top level: BBa_K1680021
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1680021/annotation2477373/range2477373/1

https://synbiohub.org/public/igem/BBa_K1680021/annotation2477373/range2477373/1
type: Range
persistent identity: range2477373
display id: range2477373
version: 1
direction: direction/forward
top level: BBa_K1680021
start: 4
end: 24
orientation: inline

Dronpa
type: SequenceAnnotation
persistent identity: annotation2477378
display id: annotation2477378
version: 1
top level: BBa_K1680021
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1680021/annotation2477378/range2477378/1

https://synbiohub.org/public/igem/BBa_K1680021/annotation2477378/range2477378/1
type: Range
persistent identity: range2477378
display id: range2477378
version: 1
direction: direction/forward
top level: BBa_K1680021
start: 1765
end: 2433
orientation: inline

Cre recombinase
type: SequenceAnnotation
persistent identity: annotation2477376
display id: annotation2477376
version: 1
top level: BBa_K1680021
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1680021/annotation2477376/range2477376/1

https://synbiohub.org/public/igem/BBa_K1680021/annotation2477376/range2477376/1
type: Range
persistent identity: range2477376
display id: range2477376
version: 1
direction: direction/forward
top level: BBa_K1680021
start: 718
end: 1746
orientation: inline

BBa_K1909014
type: ComponentDefinition
persistent identity: BBa K1909014
display id: BBa_K1909014
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Omp Promoter / eYFP
creator: Daniel Wedemeyer
created: 2016-10-13T11:00:00Z
modified: 2016-10-14T12:58:07Z
discontinued: false
dominant: false
group u list: _2375_
m user id: 29525
owner id: 29525
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Coming Soon
mutable notes: Coming Soon
mutable provenance: Composite Part
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_R0082
component: BBa_E0430
sequence annotation: BBa_R0082
sequence annotation: BBa_E0430
sequence: https://synbiohub.org/public/igem/BBa_K1909014_sequence/1

BBa_R0082
type: Component
persistent identity: component2508857
display id: component2508857
version: 1
top level: BBa_K1909014
access: public
definition: https://synbiohub.org/public/igem/BBa_R0082/1

BBa_E0430
type: Component
persistent identity: component2508867
display id: component2508867
version: 1
top level: BBa_K1909014
access: public
definition: https://synbiohub.org/public/igem/BBa_E0430/1

BBa_R0082
type: SequenceAnnotation
persistent identity: annotation2508857
display id: annotation2508857
version: 1
top level: BBa_K1909014
location: https://synbiohub.org/public/igem/BBa_K1909014/annotation2508857/range2508857/1
component: BBa_R0082

https://synbiohub.org/public/igem/BBa_K1909014/annotation2508857/range2508857/1
type: Range
persistent identity: range2508857
display id: range2508857
version: 1
direction: direction/reverse
top level: BBa_K1909014
start: 1
end: 108
orientation: inline

BBa_E0430
type: SequenceAnnotation
persistent identity: annotation2508867
display id: annotation2508867
version: 1
top level: BBa_K1909014
location: https://synbiohub.org/public/igem/BBa_K1909014/annotation2508867/range2508867/1
component: BBa_E0430

https://synbiohub.org/public/igem/BBa_K1909014/annotation2508867/range2508867/1
type: Range
persistent identity: range2508867
display id: range2508867
version: 1
direction: direction/reverse
top level: BBa_K1909014
start: 117
end: 994
orientation: inline

BBa_K1321341
type: ComponentDefinition
persistent identity: BBa K1321341
display id: BBa_K1321341
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: sfGFP fused to CBDclos in RFC 25
creator: Chris N Micklem
created: 2014-10-07T11:00:00Z
modified: 2015-06-17T12:27:02Z
discontinued: false
dominant: false
group u list: _1696_
m user id: 4206
owner id: 20830
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: asdfads
mutable notes: asdfd
mutable provenance: asdfad
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_K1321337
component: BBa_K1321002
component: BBa_B0105
sequence annotation: BBa_K1321002
sequence annotation: BBa_K1321337
sequence annotation: BBa_B0105
sequence: https://synbiohub.org/public/igem/BBa_K1321341_sequence/1

BBa_K1321337
type: Component
persistent identity: component2415088
display id: component2415088
version: 1
top level: BBa_K1321341
access: public
definition: BBa_K1321337

BBa_K1321002
type: Component
persistent identity: component2415091
display id: component2415091
version: 1
top level: BBa_K1321341
access: public
definition: https://synbiohub.org/public/igem/BBa_K1321002/1

BBa_B0105
type: Component
persistent identity: component2415090
display id: component2415090
version: 1
top level: BBa_K1321341
access: public
definition: https://synbiohub.org/public/igem/BBa_B0105/1

BBa_K1321002
type: SequenceAnnotation
persistent identity: annotation2415091
display id: annotation2415091
version: 1
top level: BBa_K1321341
location: https://synbiohub.org/public/igem/BBa_K1321341/annotation2415091/range2415091/1
component: BBa_K1321002

https://synbiohub.org/public/igem/BBa_K1321341/annotation2415091/range2415091/1
type: Range
persistent identity: range2415091
display id: range2415091
version: 1
direction: direction/reverse
top level: BBa_K1321341
start: 718
end: 1017
orientation: inline

BBa_K1321337
type: SequenceAnnotation
persistent identity: annotation2415088
display id: annotation2415088
version: 1
top level: BBa_K1321341
location: https://synbiohub.org/public/igem/BBa_K1321341/annotation2415088/range2415088/1
component: BBa_K1321337

https://synbiohub.org/public/igem/BBa_K1321341/annotation2415088/range2415088/1
type: Range
persistent identity: range2415088
display id: range2415088
version: 1
direction: direction/reverse
top level: BBa_K1321341
start: 1
end: 711
orientation: inline

BBa_B0105
type: SequenceAnnotation
persistent identity: annotation2415090
display id: annotation2415090
version: 1
top level: BBa_K1321341
location: https://synbiohub.org/public/igem/BBa_K1321341/annotation2415090/range2415090/1
component: BBa_B0105

https://synbiohub.org/public/igem/BBa_K1321341/annotation2415090/range2415090/1
type: Range
persistent identity: range2415090
display id: range2415090
version: 1
direction: direction/reverse
top level: BBa_K1321341
start: 712
end: 717
orientation: inline

BBa_K1399017
type: ComponentDefinition
persistent identity: BBa K1399017
display id: BBa_K1399017
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Plac-GFP(DAS+2)
creator: Anna Stikane
created: 2014-09-18T11:00:00Z
modified: 2015-05-08T01:10:16Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1777_
m user id: 0
owner id: 22477
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: Lactose/IPTG inducible promoter with GFP reporter tagged with NYADAS-ssrA degradation tag followed by terminator. The tagged GFP is actively degraded within cell, thus provides better temporal resolution of green fluorescence and promoter activity.
mutable notes: Part was assembled using BrickClip assembly (BBF RFC104) using other biobrick parts as templates. BrickClip assembly is a special case of more general Paperclip assembly method.[1]
mutable provenance: other biobricks
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_R0010
component: BBa_K1399007
component: BBa_B0034
component: BBa_B0015
sequence annotation: BBa_B0034
sequence annotation: BBa_R0010
sequence annotation: BBa_B0015
sequence annotation: BBa_K1399007
sequence: https://synbiohub.org/public/igem/BBa_K1399017_sequence/1

BBa_R0010
type: Component
persistent identity: component2384175
display id: component2384175
version: 1
top level: BBa_K1399017
access: public
definition: https://synbiohub.org/public/igem/BBa_R0010/1

BBa_K1399007
type: Component
persistent identity: component2384189
display id: component2384189
version: 1
top level: BBa_K1399017
access: public
definition: https://synbiohub.org/public/igem/BBa_K1399007/1

BBa_B0034
type: Component
persistent identity: component2384183
display id: component2384183
version: 1
top level: BBa_K1399017
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0015
type: Component
persistent identity: component2384196
display id: component2384196
version: 1
top level: BBa_K1399017
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2384183
display id: annotation2384183
version: 1
top level: BBa_K1399017
location: https://synbiohub.org/public/igem/BBa_K1399017/annotation2384183/range2384183/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1399017/annotation2384183/range2384183/1
type: Range
persistent identity: range2384183
display id: range2384183
version: 1
direction: direction/reverse
top level: BBa_K1399017
start: 209
end: 220
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation2384175
display id: annotation2384175
version: 1
top level: BBa_K1399017
location: https://synbiohub.org/public/igem/BBa_K1399017/annotation2384175/range2384175/1
component: BBa_R0010

https://synbiohub.org/public/igem/BBa_K1399017/annotation2384175/range2384175/1
type: Range
persistent identity: range2384175
display id: range2384175
version: 1
direction: direction/forward
top level: BBa_K1399017
start: 1
end: 200
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2384196
display id: annotation2384196
version: 1
top level: BBa_K1399017
location: https://synbiohub.org/public/igem/BBa_K1399017/annotation2384196/range2384196/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K1399017/annotation2384196/range2384196/1
type: Range
persistent identity: range2384196
display id: range2384196
version: 1
direction: direction/reverse
top level: BBa_K1399017
start: 994
end: 1122
orientation: inline

BBa_K1399007
type: SequenceAnnotation
persistent identity: annotation2384189
display id: annotation2384189
version: 1
top level: BBa_K1399017
location: https://synbiohub.org/public/igem/BBa_K1399017/annotation2384189/range2384189/1
component: BBa_K1399007

https://synbiohub.org/public/igem/BBa_K1399017/annotation2384189/range2384189/1
type: Range
persistent identity: range2384189
display id: range2384189
version: 1
direction: direction/reverse
top level: BBa_K1399017
start: 227
end: 985
orientation: inline

BBa_K1740000
type: ComponentDefinition
persistent identity: BBa K1740000
display id: BBa_K1740000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Dronpa 145N ORF (E. coli optimized)
creator: Yukina Tanimura,Keisuke Sugahara,
created: 2015-08-19T11:00:00Z
modified: 2016-01-25T11:17:08Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2162_
m user id: 4206
owner id: 25441
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This is test
mutable notes: Non
mutable provenance: synthesis DNA
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Dronpa 145N
sequence annotation: 145Lys to 145Asn
sequence annotation: strat codon
sequence: https://synbiohub.org/public/igem/BBa_K1740000_sequence/1

Dronpa 145N
type: SequenceAnnotation
persistent identity: annotation2444414
display id: annotation2444414
version: 1
top level: BBa_K1740000
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1740000/annotation2444414/range2444414/1

https://synbiohub.org/public/igem/BBa_K1740000/annotation2444414/range2444414/1
type: Range
persistent identity: range2444414
display id: range2444414
version: 1
direction: direction/forward
top level: BBa_K1740000
start: 1
end: 672
orientation: inline

145Lys to 145Asn
type: SequenceAnnotation
persistent identity: annotation2444413
display id: annotation2444413
version: 1
top level: BBa_K1740000
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K1740000/annotation2444413/range2444413/1

https://synbiohub.org/public/igem/BBa_K1740000/annotation2444413/range2444413/1
type: Range
persistent identity: range2444413
display id: range2444413
version: 1
direction: direction/forward
top level: BBa_K1740000
start: 433
end: 435
orientation: inline

strat codon
type: SequenceAnnotation
persistent identity: annotation2444415
display id: annotation2444415
version: 1
top level: BBa_K1740000
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1740000/annotation2444415/range2444415/1

https://synbiohub.org/public/igem/BBa_K1740000/annotation2444415/range2444415/1
type: Range
persistent identity: range2444415
display id: range2444415
version: 1
direction: direction/forward
top level: BBa_K1740000
start: 1
end: 3
orientation: inline

BBa_K1680019
type: ComponentDefinition
persistent identity: BBa K1680019
display id: BBa_K1680019
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Cre-dronpa fusion
creator: Nicolai von Kuegelgen
created: 2015-09-16T11:00:00Z
modified: 2016-01-26T10:15:55Z
discontinued: false
dominant: false
group u list: _2098_
m user id: 4206
owner id: 26366
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Protein coding region for a fusion construct of Cre Recombinase and 145N Dronpa with 12 amino acid linker. Oligomerisation of Dronpa should stop Cre from accessing DNA in the nucleus thereby inhibiting it until light deactivation of dronpa.
mutable notes: 3A assembly using RFC25
mutable provenance: gBlocks designed by Team Tuebingen
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: K1680002
sequence annotation: K1680007
sequence annotation: K1680006
sequence: https://synbiohub.org/public/igem/BBa_K1680019_sequence/1

K1680002
type: SequenceAnnotation
persistent identity: annotation2477417
display id: annotation2477417
version: 1
top level: BBa_K1680019
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1680019/annotation2477417/range2477417/1

https://synbiohub.org/public/igem/BBa_K1680019/annotation2477417/range2477417/1
type: Range
persistent identity: range2477417
display id: range2477417
version: 1
direction: direction/forward
top level: BBa_K1680019
start: 1060
end: 1095
orientation: inline

K1680007
type: SequenceAnnotation
persistent identity: annotation2477416
display id: annotation2477416
version: 1
top level: BBa_K1680019
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1680019/annotation2477416/range2477416/1

https://synbiohub.org/public/igem/BBa_K1680019/annotation2477416/range2477416/1
type: Range
persistent identity: range2477416
display id: range2477416
version: 1
direction: direction/forward
top level: BBa_K1680019
start: 31
end: 1059
orientation: inline

K1680006
type: SequenceAnnotation
persistent identity: annotation2477418
display id: annotation2477418
version: 1
top level: BBa_K1680019
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1680019/annotation2477418/range2477418/1

https://synbiohub.org/public/igem/BBa_K1680019/annotation2477418/range2477418/1
type: Range
persistent identity: range2477418
display id: range2477418
version: 1
direction: direction/forward
top level: BBa_K1680019
start: 1096
end: 1764
orientation: inline

K1680002
type: SequenceAnnotation
persistent identity: annotation2477415
display id: annotation2477415
version: 1
top level: BBa_K1680019
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1680019/annotation2477415/range2477415/1

https://synbiohub.org/public/igem/BBa_K1680019/annotation2477415/range2477415/1
type: Range
persistent identity: range2477415
display id: range2477415
version: 1
direction: direction/forward
top level: BBa_K1680019
start: 1
end: 30
orientation: inline

BBa_K1399018
type: ComponentDefinition
persistent identity: BBa K1399018
display id: BBa_K1399018
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Plac-GFP(DAS)
creator: Anna Stikane
created: 2014-09-18T11:00:00Z
modified: 2015-05-08T01:10:16Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1777_
m user id: 0
owner id: 22477
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: Lactose/IPTG inducible promoter with GFP reporter tagged with DAS-ssrA degradation tag followed by terminator. The tagged GFP is actively degraded within cell, thus provides better temporal resolution of green fluorescence and promoter activity. Presence of SspB is crucial for effective protein degradation.
mutable notes: Part was assembled using BrickClip assembly (BBF RFC104) using other biobrick parts as templates. BrickClip assembly is a special case of more general Paperclip assembly method.[1]
mutable provenance: other biobricks
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_B0034
component: BBa_B0015
component: BBa_K1399008
component: BBa_R0010
sequence annotation: BBa_B0015
sequence annotation: BBa_B0034
sequence annotation: BBa_R0010
sequence annotation: BBa_K1399008
sequence: https://synbiohub.org/public/igem/BBa_K1399018_sequence/1

BBa_B0034
type: Component
persistent identity: component2384205
display id: component2384205
version: 1
top level: BBa_K1399018
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0015
type: Component
persistent identity: component2384218
display id: component2384218
version: 1
top level: BBa_K1399018
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_K1399008
type: Component
persistent identity: component2384211
display id: component2384211
version: 1
top level: BBa_K1399018
access: public
definition: https://synbiohub.org/public/igem/BBa_K1399008/1

BBa_R0010
type: Component
persistent identity: component2384197
display id: component2384197
version: 1
top level: BBa_K1399018
access: public
definition: https://synbiohub.org/public/igem/BBa_R0010/1

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2384218
display id: annotation2384218
version: 1
top level: BBa_K1399018
location: https://synbiohub.org/public/igem/BBa_K1399018/annotation2384218/range2384218/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K1399018/annotation2384218/range2384218/1
type: Range
persistent identity: range2384218
display id: range2384218
version: 1
direction: direction/reverse
top level: BBa_K1399018
start: 988
end: 1116
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2384205
display id: annotation2384205
version: 1
top level: BBa_K1399018
location: https://synbiohub.org/public/igem/BBa_K1399018/annotation2384205/range2384205/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1399018/annotation2384205/range2384205/1
type: Range
persistent identity: range2384205
display id: range2384205
version: 1
direction: direction/reverse
top level: BBa_K1399018
start: 209
end: 220
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation2384197
display id: annotation2384197
version: 1
top level: BBa_K1399018
location: https://synbiohub.org/public/igem/BBa_K1399018/annotation2384197/range2384197/1
component: BBa_R0010

https://synbiohub.org/public/igem/BBa_K1399018/annotation2384197/range2384197/1
type: Range
persistent identity: range2384197
display id: range2384197
version: 1
direction: direction/forward
top level: BBa_K1399018
start: 1
end: 200
orientation: inline

BBa_K1399008
type: SequenceAnnotation
persistent identity: annotation2384211
display id: annotation2384211
version: 1
top level: BBa_K1399018
location: https://synbiohub.org/public/igem/BBa_K1399018/annotation2384211/range2384211/1
component: BBa_K1399008

https://synbiohub.org/public/igem/BBa_K1399018/annotation2384211/range2384211/1
type: Range
persistent identity: range2384211
display id: range2384211
version: 1
direction: direction/reverse
top level: BBa_K1399018
start: 227
end: 979
orientation: inline

BBa_K1399022
type: ComponentDefinition
persistent identity: BBa K1399022
display id: BBa_K1399022
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Ptet-GFP(DAS+2)
creator: Anna Stikane
created: 2014-09-18T11:00:00Z
modified: 2015-05-08T01:10:16Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1777_
m user id: 0
owner id: 22477
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: Tetracycline/aTc inducible promoter with GFP reporter tagged with NYADAS-ssrA degradation tag followed by terminator. The tagged GFP is actively degraded within cell, thus provides better temporal resolution of green fluorescence and promoter activity.
mutable notes: Part was assembled using BrickClip assembly (BBF RFC104) using other biobrick parts as templates. BrickClip assembly is a special case of more general Paperclip assembly method.[1]
mutable provenance: other parts
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_B0015
component: BBa_R0040
component: BBa_B0034
component: BBa_K1399007
sequence annotation: BBa_B0034
sequence annotation: BBa_B0015
sequence annotation: BBa_K1399007
sequence annotation: BBa_R0040
sequence: https://synbiohub.org/public/igem/BBa_K1399022_sequence/1

BBa_B0015
type: Component
persistent identity: component2384298
display id: component2384298
version: 1
top level: BBa_K1399022
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_R0040
type: Component
persistent identity: component2384279
display id: component2384279
version: 1
top level: BBa_K1399022
access: public
definition: https://synbiohub.org/public/igem/BBa_R0040/1

BBa_B0034
type: Component
persistent identity: component2384285
display id: component2384285
version: 1
top level: BBa_K1399022
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K1399007
type: Component
persistent identity: component2384291
display id: component2384291
version: 1
top level: BBa_K1399022
access: public
definition: https://synbiohub.org/public/igem/BBa_K1399007/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2384285
display id: annotation2384285
version: 1
top level: BBa_K1399022
location: https://synbiohub.org/public/igem/BBa_K1399022/annotation2384285/range2384285/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1399022/annotation2384285/range2384285/1
type: Range
persistent identity: range2384285
display id: range2384285
version: 1
direction: direction/reverse
top level: BBa_K1399022
start: 63
end: 74
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2384298
display id: annotation2384298
version: 1
top level: BBa_K1399022
location: https://synbiohub.org/public/igem/BBa_K1399022/annotation2384298/range2384298/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K1399022/annotation2384298/range2384298/1
type: Range
persistent identity: range2384298
display id: range2384298
version: 1
direction: direction/reverse
top level: BBa_K1399022
start: 848
end: 976
orientation: inline

BBa_K1399007
type: SequenceAnnotation
persistent identity: annotation2384291
display id: annotation2384291
version: 1
top level: BBa_K1399022
location: https://synbiohub.org/public/igem/BBa_K1399022/annotation2384291/range2384291/1
component: BBa_K1399007

https://synbiohub.org/public/igem/BBa_K1399022/annotation2384291/range2384291/1
type: Range
persistent identity: range2384291
display id: range2384291
version: 1
direction: direction/reverse
top level: BBa_K1399022
start: 81
end: 839
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation2384279
display id: annotation2384279
version: 1
top level: BBa_K1399022
location: https://synbiohub.org/public/igem/BBa_K1399022/annotation2384279/range2384279/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_K1399022/annotation2384279/range2384279/1
type: Range
persistent identity: range2384279
display id: range2384279
version: 1
direction: direction/forward
top level: BBa_K1399022
start: 1
end: 54
orientation: inline

BBa_K2023016
type: ComponentDefinition
persistent identity: BBa K2023016
display id: BBa_K2023016
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: GFP coding device with Pu
creator: C??lia Chenebault
created: 2016-10-13T11:00:00Z
modified: 2016-10-20T04:05:09Z
discontinued: false
dominant: false
group u list: _2490_
m user id: 30356
owner id: 30230
owning group id: 9
status: status/Planning
bookmark: false
mutable description: GFP coding device with Pu
mutable notes: blabla
mutable provenance: Pu promoter and RBS from GLuc coding device with Pu (BBa_K2023003) and GFP reporter for RHS of library test constructs (BBa_I13401)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_E0040
component: BBa_I723020
component: BBa_B0034
component: BBa_B0015
sequence annotation: BBa_B0034
sequence annotation: BBa_B0015
sequence annotation: BBa_I723020
sequence annotation: BBa_E0040
sequence: https://synbiohub.org/public/igem/BBa_K2023016_sequence/1

BBa_E0040
type: Component
persistent identity: component2518998
display id: component2518998
version: 1
top level: BBa_K2023016
access: public
definition: GFP

BBa_I723020
type: Component
persistent identity: component2518994
display id: component2518994
version: 1
top level: BBa_K2023016
access: public
definition: https://synbiohub.org/public/igem/BBa_I723020/1

BBa_B0034
type: Component
persistent identity: component2518996
display id: component2518996
version: 1
top level: BBa_K2023016
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0015
type: Component
persistent identity: component2519005
display id: component2519005
version: 1
top level: BBa_K2023016
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2518996
display id: annotation2518996
version: 1
top level: BBa_K2023016
location: https://synbiohub.org/public/igem/BBa_K2023016/annotation2518996/range2518996/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K2023016/annotation2518996/range2518996/1
type: Range
persistent identity: range2518996
display id: range2518996
version: 1
direction: direction/reverse
top level: BBa_K2023016
start: 329
end: 340
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2519005
display id: annotation2519005
version: 1
top level: BBa_K2023016
location: https://synbiohub.org/public/igem/BBa_K2023016/annotation2519005/range2519005/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K2023016/annotation2519005/range2519005/1
type: Range
persistent identity: range2519005
display id: range2519005
version: 1
direction: direction/reverse
top level: BBa_K2023016
start: 1075
end: 1203
orientation: inline

BBa_I723020
type: SequenceAnnotation
persistent identity: annotation2518994
display id: annotation2518994
version: 1
top level: BBa_K2023016
location: https://synbiohub.org/public/igem/BBa_K2023016/annotation2518994/range2518994/1
component: BBa_I723020

https://synbiohub.org/public/igem/BBa_K2023016/annotation2518994/range2518994/1
type: Range
persistent identity: range2518994
display id: range2518994
version: 1
direction: direction/reverse
top level: BBa_K2023016
start: 1
end: 320
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation2518998
display id: annotation2518998
version: 1
top level: BBa_K2023016
location: https://synbiohub.org/public/igem/BBa_K2023016/annotation2518998/range2518998/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_K2023016/annotation2518998/range2518998/1
type: Range
persistent identity: range2518998
display id: range2518998
version: 1
direction: direction/reverse
top level: BBa_K2023016
start: 347
end: 1066
orientation: inline

BBa_K1650038
type: ComponentDefinition
persistent identity: BBa K1650038
display id: BBa_K1650038
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: GFP-His
creator: Alexandra Richter
created: 2015-09-17T11:00:00Z
modified: 2016-01-21T02:24:59Z
discontinued: false
dominant: false
group u list: _2067_
m user id: 4206
owner id: 27246
owning group id: 9
status: status/Planning
bookmark: false
mutable description: test
mutable notes: test
mutable provenance: test
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: BBa_K1650038
sequence: https://synbiohub.org/public/igem/BBa_K1650038_sequence/1

BBa_K1650038
type: SequenceAnnotation
persistent identity: annotation2474934
display id: annotation2474934
version: 1
top level: BBa_K1650038
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1650038/annotation2474934/range2474934/1

https://synbiohub.org/public/igem/BBa_K1650038/annotation2474934/range2474934/1
type: Range
persistent identity: range2474934
display id: range2474934
version: 1
direction: direction/forward
top level: BBa_K1650038
start: 1
end: 1159
orientation: inline

BBa_K1700000
type: ComponentDefinition
persistent identity: BBa K1700000
display id: BBa_K1700000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Use toehold to regulate GFP
creator: Meixi Liu
created: 2015-09-16T11:00:00Z
modified: 2015-09-17T09:57:13Z
discontinued: false
dominant: false
group u list: _2118_
m user id: 28512
owner id: 28512
owning group id: 9
status: status/Planning
bookmark: false
mutable description: The structure of toehold switch is similar to hairpin, except it has a loop at the top as ???toehold???. Toehold switch functions as riboregulator through linear-linear interaction between RNAs. When target RNA appears, it will bind one of the toehold switch stems and open the loop, exposing the RBS. Toehold switch systems are composed of two RNA strands referred to as the switch and trigger. The switch RNA contains the coding sequence of the gene being regulated. Upstream of this coding sequence is a hairpin-based processing module containing both a strong RBS and a start codon that is followed by a common 21 nt linker sequence coding for low-molecular-weight amino acids added to the N terminus of the gene of interest. A single-stranded toehold sequence at the 50 end of the hairpin module provides the initial binding site for the trigger RNA strand. This trigger molecule contains an extended single-stranded region that completes a branch migration process with the hairpin to expose the RBS and start codon, thereby initiating translation of the gene of interest. The trigger RNA of our toehold is miRNA-144.When adding it to the system, GFP can be expressed.
mutable notes: Toehold can be open by trigger RNA, thus letting GFP express.
mutable provenance: We synthesis the part.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: TAA stop codon
sequence annotation: GFP coding sequence
sequence annotation: ATG start codon
sequence annotation: toehold
sequence: https://synbiohub.org/public/igem/BBa_K1700000_sequence/1

TAA stop codon
type: SequenceAnnotation
persistent identity: annotation2467633
display id: annotation2467633
version: 1
top level: BBa_K1700000
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1700000/annotation2467633/range2467633/1

https://synbiohub.org/public/igem/BBa_K1700000/annotation2467633/range2467633/1
type: Range
persistent identity: range2467633
display id: range2467633
version: 1
direction: direction/forward
top level: BBa_K1700000
start: 818
end: 820
orientation: inline

GFP coding sequence
type: SequenceAnnotation
persistent identity: annotation2467632
display id: annotation2467632
version: 1
top level: BBa_K1700000
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1700000/annotation2467632/range2467632/1

https://synbiohub.org/public/igem/BBa_K1700000/annotation2467632/range2467632/1
type: Range
persistent identity: range2467632
display id: range2467632
version: 1
direction: direction/forward
top level: BBa_K1700000
start: 101
end: 820
orientation: inline

ATG start codon
type: SequenceAnnotation
persistent identity: annotation2467629
display id: annotation2467629
version: 1
top level: BBa_K1700000
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1700000/annotation2467629/range2467629/1

https://synbiohub.org/public/igem/BBa_K1700000/annotation2467629/range2467629/1
type: Range
persistent identity: range2467629
display id: range2467629
version: 1
direction: direction/forward
top level: BBa_K1700000
start: 156
end: 158
orientation: inline

toehold
type: SequenceAnnotation
persistent identity: annotation2467606
display id: annotation2467606
version: 1
top level: BBa_K1700000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1700000/annotation2467606/range2467606/1

https://synbiohub.org/public/igem/BBa_K1700000/annotation2467606/range2467606/1
type: Range
persistent identity: range2467606
display id: range2467606
version: 1
direction: direction/forward
top level: BBa_K1700000
start: 1
end: 100
orientation: inline

BBa_K106028
type: ComponentDefinition
persistent identity: BBa K106028
display id: BBa_K106028
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: GFP, AarI AB part
creator: Andrej Ondracka
created: 2008-10-24T11:00:00Z
modified: 2016-01-26T09:49:32Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _226_
m user id: 4206
owner id: 3416
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This is coding sequence of GFP (yeast optimized codons).
mutable notes: .
mutable provenance: .
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K106028_sequence/1

GFP_Freibu
type: ComponentDefinition
persistent identity: BBa K863120
display id: BBa_K863120
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (Freiburg-Standard)
creator: Moritz M??ller
created: 2012-09-19T11:00:00Z
modified: 2016-01-25T01:32:59Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1123_
m user id: 4206
owner id: 13080
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Untagged version of gfp from Repressilator reporter. See the [http://partsregistry.org/wiki/index.php?title=Part:BBa_E0040 untagged version] for source information.
mutable notes: Added Freiburg-restriction-sites for in frame assembly.
mutable provenance: [http://partsregistry.org/Part:BBa_I13522 BBa_I13522]
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: BBa_E0040
sequence annotation: ATG
sequence annotation: AgeI
sequence annotation: TAA
sequence annotation: NgoMIV
sequence: https://synbiohub.org/public/igem/BBa_K863120_sequence/1

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation2187773
display id: annotation2187773
version: 1
top level: GFP_Freibu
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K863120/annotation2187773/range2187773/1

https://synbiohub.org/public/igem/BBa_K863120/annotation2187773/range2187773/1
type: Range
persistent identity: range2187773
display id: range2187773
version: 1
direction: direction/forward
top level: GFP_Freibu
start: 12
end: 720
orientation: inline

ATG
type: SequenceAnnotation
persistent identity: annotation2187769
display id: annotation2187769
version: 1
top level: GFP_Freibu
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K863120/annotation2187769/range2187769/1

https://synbiohub.org/public/igem/BBa_K863120/annotation2187769/range2187769/1
type: Range
persistent identity: range2187769
display id: range2187769
version: 1
direction: direction/forward
top level: GFP_Freibu
start: 1
end: 3
orientation: inline

AgeI
type: SequenceAnnotation
persistent identity: annotation2187772
display id: annotation2187772
version: 1
top level: GFP_Freibu
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K863120/annotation2187772/range2187772/1

https://synbiohub.org/public/igem/BBa_K863120/annotation2187772/range2187772/1
type: Range
persistent identity: range2187772
display id: range2187772
version: 1
direction: direction/forward
top level: GFP_Freibu
start: 721
end: 726
orientation: inline

TAA
type: SequenceAnnotation
persistent identity: annotation2187771
display id: annotation2187771
version: 1
top level: GFP_Freibu
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K863120/annotation2187771/range2187771/1

https://synbiohub.org/public/igem/BBa_K863120/annotation2187771/range2187771/1
type: Range
persistent identity: range2187771
display id: range2187771
version: 1
direction: direction/forward
top level: GFP_Freibu
start: 727
end: 729
orientation: inline

NgoMIV
type: SequenceAnnotation
persistent identity: annotation2187770
display id: annotation2187770
version: 1
top level: GFP_Freibu
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K863120/annotation2187770/range2187770/1

https://synbiohub.org/public/igem/BBa_K863120/annotation2187770/range2187770/1
type: Range
persistent identity: range2187770
display id: range2187770
version: 1
direction: direction/forward
top level: GFP_Freibu
start: 4
end: 11
orientation: inline

BBa_K294055
type: ComponentDefinition
persistent identity: BBa K294055
display id: BBa_K294055
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: GFP RFP Hybrid
creator: Ryoji Sekine
created: 2009-06-26T11:00:00Z
modified: 2016-01-25T02:34:10Z
discontinued: false
dominant: false
group u list: _397_
m user id: 4206
owner id: 5313
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This is a test. It may not bright.
mutable notes: E0040
mutable provenance: ATGCATATATATAATTT
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Hoge
sequence annotation: hogehoge
sequence: https://synbiohub.org/public/igem/BBa_K294055_sequence/1

Hoge
type: SequenceAnnotation
persistent identity: annotation2006753
display id: annotation2006753
version: 1
top level: BBa_K294055
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K294055/annotation2006753/range2006753/1

https://synbiohub.org/public/igem/BBa_K294055/annotation2006753/range2006753/1
type: Range
persistent identity: range2006753
display id: range2006753
version: 1
direction: direction/forward
top level: BBa_K294055
start: 200
end: 500
orientation: inline

hogehoge
type: SequenceAnnotation
persistent identity: annotation2006752
display id: annotation2006752
version: 1
top level: BBa_K294055
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K294055/annotation2006752/range2006752/1

https://synbiohub.org/public/igem/BBa_K294055/annotation2006752/range2006752/1
type: Range
persistent identity: range2006752
display id: range2006752
version: 1
direction: direction/forward
top level: BBa_K294055
start: 1
end: 100
orientation: inline

BBa_K1673410
type: ComponentDefinition
persistent identity: BBa K1673410
display id: BBa_K1673410
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: GFP (Mutation Optimized)
creator: Jarrod Shilts
created: 2015-09-17T11:00:00Z
modified: 2016-01-21T02:30:04Z
discontinued: false
dominant: false
group u list: _2091_
m user id: 4206
owner id: 19415
owning group id: 9
status: status/Planning
bookmark: false
mutable description: GFP, based on E0040, with overall mutation minimized
mutable notes: Sequence produced by the Vanderbilt iGEM 2015 mutation optimization software
mutable provenance: DNA Synthesis
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1673410_sequence/1

BBa_K1709003
type: ComponentDefinition
persistent identity: BBa K1709003
display id: BBa_K1709003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CheZ-GFP
creator: Astrid Deryckere
created: 2015-09-15T11:00:00Z
modified: 2015-09-26T08:47:17Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2129_
m user id: 25389
owner id: 25389
owning group id: 9
status: status/Planning
bookmark: true
mutable description: Upon recognition of a small molecule (e.g. nutrients or toxins), chemoreceptors transduce a signal through a set of Che proteins. This group of methylesterases and phosphatases regulates the rotation of the flagella. At the end of the chain of Che proteins is CheY. In phosphorylated state, CheY binds the flagella and causes the cells to tumble. The phosphatase CheZ dephosphorylates CheY, inducing dissociation of CheY from the flagella enabling the cells to swim. Cells lacking the CheZ protein are unable to swim and will tumble excessively and incessantly.
mutable notes: 1 bp in BBa_K629003 was adapted to create an original restriciton site in the plasmid. CheZ was fused to GFP (BBa_K082003) and its LVA tag was codon-optimized to avoid introducing artificial DNA repetitions.
mutable provenance: DNA sequences originate from the E. Coli genome and previous biobrick sequences. The ribosome binding site was chosen from the Anderson RBS family.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_J61101
component: BBa_K1709002
sequence annotation: BBa_J61101
sequence annotation: BBa_K1709002
sequence: https://synbiohub.org/public/igem/BBa_K1709003_sequence/1

BBa_J61101
type: Component
persistent identity: component2472546
display id: component2472546
version: 1
top level: BBa_K1709003
access: public
definition: https://synbiohub.org/public/igem/BBa_J61101/1

BBa_K1709002
type: Component
persistent identity: component2472552
display id: component2472552
version: 1
top level: BBa_K1709003
access: public
definition: CheZ-GFP

BBa_J61101
type: SequenceAnnotation
persistent identity: annotation2472546
display id: annotation2472546
version: 1
top level: BBa_K1709003
location: https://synbiohub.org/public/igem/BBa_K1709003/annotation2472546/range2472546/1
component: BBa_J61101

https://synbiohub.org/public/igem/BBa_K1709003/annotation2472546/range2472546/1
type: Range
persistent identity: range2472546
display id: range2472546
version: 1
direction: direction/reverse
top level: BBa_K1709003
start: 1
end: 12
orientation: inline

BBa_K1709002
type: SequenceAnnotation
persistent identity: annotation2472552
display id: annotation2472552
version: 1
top level: BBa_K1709003
location: https://synbiohub.org/public/igem/BBa_K1709003/annotation2472552/range2472552/1
component: BBa_K1709002

https://synbiohub.org/public/igem/BBa_K1709003/annotation2472552/range2472552/1
type: Range
persistent identity: range2472552
display id: range2472552
version: 1
direction: direction/reverse
top level: BBa_K1709003
start: 19
end: 1419
orientation: inline

MymTsfGFP
type: ComponentDefinition
persistent identity: BBa K1980003
display id: BBa_K1980003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: MymT sfGFP
creator: Sam Garforth
created: 2016-10-10T11:00:00Z
modified: 2016-10-20T07:26:41Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2447_
m user id: 29607
owner id: 29607
owning group id: 9
status: status/Planning
bookmark: false
mutable description: MymT is a small prokaryotic copper metallothein discovered in Mycobacterium tuberculosis. It is believed that the protein may help the bacterium survive copper toxicity, though deleting the gene had no effect on pathogenicity in mice. It can bind up to 7 copper ions but has a preference for 4-6. This version has a C terminal sfGFP with a C terminal hexahistidine tag for purification. The MymT and sfGFP are separated by a small flexible linker. This part has been codon optimised for E. coli.
mutable notes: This protein has a C terminal hexahistidine tag for purification and has been codon optimised for E. coli.
mutable provenance: Mycobacterium tuberculosis. Ordered as codon optimised DNA.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: linker
sequence annotation: Hexahistidine
sequence annotation: MymT
sequence annotation: sfGFP
sequence annotation: Double stop
sequence: https://synbiohub.org/public/igem/BBa_K1980003_sequence/1

linker
type: SequenceAnnotation
persistent identity: annotation2495244
display id: annotation2495244
version: 1
top level: MymTsfGFP
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1980003/annotation2495244/range2495244/1

https://synbiohub.org/public/igem/BBa_K1980003/annotation2495244/range2495244/1
type: Range
persistent identity: range2495244
display id: range2495244
version: 1
direction: direction/forward
top level: MymTsfGFP
start: 160
end: 177
orientation: inline

Hexahistidine
type: SequenceAnnotation
persistent identity: annotation2495246
display id: annotation2495246
version: 1
top level: MymTsfGFP
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1980003/annotation2495246/range2495246/1

https://synbiohub.org/public/igem/BBa_K1980003/annotation2495246/range2495246/1
type: Range
persistent identity: range2495246
display id: range2495246
version: 1
direction: direction/forward
top level: MymTsfGFP
start: 889
end: 906
orientation: inline

MymT
type: SequenceAnnotation
persistent identity: annotation2495243
display id: annotation2495243
version: 1
top level: MymTsfGFP
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1980003/annotation2495243/range2495243/1

https://synbiohub.org/public/igem/BBa_K1980003/annotation2495243/range2495243/1
type: Range
persistent identity: range2495243
display id: range2495243
version: 1
direction: direction/forward
top level: MymTsfGFP
start: 1
end: 159
orientation: inline

sfGFP
type: SequenceAnnotation
persistent identity: annotation2495245
display id: annotation2495245
version: 1
top level: MymTsfGFP
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1980003/annotation2495245/range2495245/1

https://synbiohub.org/public/igem/BBa_K1980003/annotation2495245/range2495245/1
type: Range
persistent identity: range2495245
display id: range2495245
version: 1
direction: direction/forward
top level: MymTsfGFP
start: 178
end: 888
orientation: inline

Double stop
type: SequenceAnnotation
persistent identity: annotation2495248
display id: annotation2495248
version: 1
top level: MymTsfGFP
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1980003/annotation2495248/range2495248/1

https://synbiohub.org/public/igem/BBa_K1980003/annotation2495248/range2495248/1
type: Range
persistent identity: range2495248
display id: range2495248
version: 1
direction: direction/forward
top level: MymTsfGFP
start: 906
end: 912
orientation: inline

BBa_K1486028
type: ComponentDefinition
persistent identity: BBa K1486028
display id: BBa_K1486028
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Yeast optimized sfGFP N-terminus (1-214)
creator: EPFL iGem team 2014
created: 2014-09-26T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: split N term of sfGFP To edit
mutable notes: To edit
mutable provenance: To edit
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000324
role: partType/Tag
sequence annotation: yeast optimized sfGFP N-terminus
sequence: https://synbiohub.org/public/igem/BBa_K1486028_sequence/1

yeast optimized sfGFP N-terminus
type: SequenceAnnotation
persistent identity: annotation2417081
display id: annotation2417081
version: 1
top level: BBa_K1486028
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1486028/annotation2417081/range2417081/1

https://synbiohub.org/public/igem/BBa_K1486028/annotation2417081/range2417081/1
type: Range
persistent identity: range2417081
display id: range2417081
version: 1
direction: direction/forward
top level: BBa_K1486028
start: 1
end: 642
orientation: inline

BBa_K125500
type: ComponentDefinition
persistent identity: BBa K125500
display id: BBa_K125500
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: GFP fusion brick
creator: Grace Kwan
created: 2008-07-24T11:00:00Z
modified: 2016-01-25T02:34:56Z
discontinued: false
dominant: false
experience: experience/None
group u list: _179_
m user id: 4206
owner id: 3229
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: The first two nucleotides of the GFP start codon were removed to create this fusion brick.
mutable provenance: The GFP fusion brick was derived from the GFP BioBrick, [http://partsregistry.org/Part:BBa_E0040 BBa_E0040].
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: GFP fusion brick
sequence annotation: deletion of TG
sequence: https://synbiohub.org/public/igem/BBa_K125500_sequence/1

GFP fusion brick
type: SequenceAnnotation
persistent identity: annotation1968260
display id: annotation1968260
version: 1
top level: BBa_K125500
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K125500/annotation1968260/range1968260/1

https://synbiohub.org/public/igem/BBa_K125500/annotation1968260/range1968260/1
type: Range
persistent identity: range1968260
display id: range1968260
version: 1
direction: direction/forward
top level: BBa_K125500
start: 1
end: 718
orientation: inline

deletion of TG
type: SequenceAnnotation
persistent identity: annotation1969908
display id: annotation1969908
version: 1
top level: BBa_K125500
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_K125500/annotation1969908/range1969908/1

https://synbiohub.org/public/igem/BBa_K125500/annotation1969908/range1969908/1
type: Range
persistent identity: range1969908
display id: range1969908
version: 1
direction: direction/forward
top level: BBa_K125500
start: 1
end: 1
orientation: inline

BBa_K180001
type: ComponentDefinition
persistent identity: BBa K180001
display id: BBa_K180001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Green fluorescent protein (+LVA)
creator: Richard Wilson, Stephanie Chambers
created: 2009-10-11T11:00:00Z
modified: 2016-01-25T01:37:25Z
discontinued: false
dominant: false
experience: experience/None
group u list: _278_
m user id: 4206
owner id: 4082
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: Based on the part E0040, but with an LVA degradation tail attached.
mutable notes: LVA degradation tail and prefix/suffix addition by sequential PCR amplifications with specially designed primers.
mutable provenance: Based upon GFP from E0040, with LVA degradation tail attached.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: LVA
sequence annotation: K180001
sequence annotation: GFPmut3b
sequence: https://synbiohub.org/public/igem/BBa_K180001_sequence/1

LVA
type: SequenceAnnotation
persistent identity: annotation2034727
display id: annotation2034727
version: 1
top level: BBa_K180001
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K180001/annotation2034727/range2034727/1

https://synbiohub.org/public/igem/BBa_K180001/annotation2034727/range2034727/1
type: Range
persistent identity: range2034727
display id: range2034727
version: 1
direction: direction/forward
top level: BBa_K180001
start: 721
end: 754
orientation: inline

K180001
type: SequenceAnnotation
persistent identity: annotation2034728
display id: annotation2034728
version: 1
top level: BBa_K180001
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K180001/annotation2034728/range2034728/1

https://synbiohub.org/public/igem/BBa_K180001/annotation2034728/range2034728/1
type: Range
persistent identity: range2034728
display id: range2034728
version: 1
direction: direction/forward
top level: BBa_K180001
start: 1
end: 754
orientation: inline

GFPmut3b
type: SequenceAnnotation
persistent identity: annotation2034726
display id: annotation2034726
version: 1
top level: BBa_K180001
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K180001/annotation2034726/range2034726/1

https://synbiohub.org/public/igem/BBa_K180001/annotation2034726/range2034726/1
type: Range
persistent identity: range2034726
display id: range2034726
version: 1
direction: direction/forward
top level: BBa_K180001
start: 1
end: 720
orientation: inline

BBa_J52026
type: ComponentDefinition
persistent identity: BBa J52026
display id: BBa_J52026
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: dnMyD88-linker-GFP
creator: Jelka Pohar
created: 2006-10-14T11:00:00Z
modified: 2016-01-26T09:39:38Z
discontinued: false
dominant: false
group u list: _80_
m user id: 4206
owner id: 855
owning group id: 80
sample status: In stock
status: status/Available
bookmark: false
mutable description: dnMyD88 is a dominant negative form of protein MyD88 (an important protein in signaling cascade through TLR4 receptor). dnMyD88 is linked to GFP (acts as a reporter) through six aminoacid long protein linker.
mutable notes: dnMyD88-linker-GFP was made by using PCR overlap extension technique. This part is cloned in BioBrick vector pSB1AK3. Original sequence has PstI restriction site so we have to introduce a mutation without affect on amino acid sequence.
mutable provenance: dnMyD88- GFP- more to come
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: dnMyD88
sequence annotation: linker
sequence annotation: dnMyD88-linker-GFP
sequence annotation: GFP
sequence: https://synbiohub.org/public/igem/BBa_J52026_sequence/1

dnMyD88
type: SequenceAnnotation
persistent identity: annotation1903008
display id: annotation1903008
version: 1
top level: BBa_J52026
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J52026/annotation1903008/range1903008/1

https://synbiohub.org/public/igem/BBa_J52026/annotation1903008/range1903008/1
type: Range
persistent identity: range1903008
display id: range1903008
version: 1
direction: direction/forward
top level: BBa_J52026
start: 1
end: 417
orientation: inline

linker
type: SequenceAnnotation
persistent identity: annotation1903009
display id: annotation1903009
version: 1
top level: BBa_J52026
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_J52026/annotation1903009/range1903009/1

https://synbiohub.org/public/igem/BBa_J52026/annotation1903009/range1903009/1
type: Range
persistent identity: range1903009
display id: range1903009
version: 1
direction: direction/forward
top level: BBa_J52026
start: 418
end: 435
orientation: inline

dnMyD88-linker-GFP
type: SequenceAnnotation
persistent identity: annotation1903011
display id: annotation1903011
version: 1
top level: BBa_J52026
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_J52026/annotation1903011/range1903011/1

https://synbiohub.org/public/igem/BBa_J52026/annotation1903011/range1903011/1
type: Range
persistent identity: range1903011
display id: range1903011
version: 1
direction: direction/forward
top level: BBa_J52026
start: 1
end: 1155
orientation: inline

GFP
type: SequenceAnnotation
persistent identity: annotation1903010
display id: annotation1903010
version: 1
top level: BBa_J52026
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J52026/annotation1903010/range1903010/1

https://synbiohub.org/public/igem/BBa_J52026/annotation1903010/range1903010/1
type: Range
persistent identity: range1903010
display id: range1903010
version: 1
direction: direction/forward
top level: BBa_J52026
start: 436
end: 1155
orientation: inline

BBa_K1486040
type: ComponentDefinition
persistent identity: BBa K1486040
display id: BBa_K1486040
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: sfGFPN + CpxR
creator: EPFL iGem team 2014
created: 2014-09-26T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: To edit
mutable notes: To edit
mutable provenance: To edit
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_I0500
component: BBa_K1486038
component: BBa_K1486037
component: BBa_K1486000
component: BBa_B0034
sequence annotation: BBa_I0500
sequence annotation: BBa_K1486000
sequence annotation: BBa_K1486037
sequence annotation: BBa_B0034
sequence annotation: BBa_K1486038
sequence: https://synbiohub.org/public/igem/BBa_K1486040_sequence/1

BBa_I0500
type: Component
persistent identity: component2387185
display id: component2387185
version: 1
top level: BBa_K1486040
access: public
definition: https://synbiohub.org/public/igem/BBa_I0500/1

BBa_K1486038
type: Component
persistent identity: component2387188
display id: component2387188
version: 1
top level: BBa_K1486040
access: public
definition: BBa_K1486038

BBa_K1486037
type: Component
persistent identity: component2387189
display id: component2387189
version: 1
top level: BBa_K1486040
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486037/1

BBa_K1486000
type: Component
persistent identity: component2387193
display id: component2387193
version: 1
top level: BBa_K1486040
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486000/1

BBa_B0034
type: Component
persistent identity: component2387187
display id: component2387187
version: 1
top level: BBa_K1486040
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_I0500
type: SequenceAnnotation
persistent identity: annotation2387185
display id: annotation2387185
version: 1
top level: BBa_K1486040
location: https://synbiohub.org/public/igem/BBa_K1486040/annotation2387185/range2387185/1
component: BBa_I0500

https://synbiohub.org/public/igem/BBa_K1486040/annotation2387185/range2387185/1
type: Range
persistent identity: range2387185
display id: range2387185
version: 1
direction: direction/reverse
top level: BBa_K1486040
start: 1
end: 1210
orientation: inline

BBa_K1486000
type: SequenceAnnotation
persistent identity: annotation2387193
display id: annotation2387193
version: 1
top level: BBa_K1486040
location: https://synbiohub.org/public/igem/BBa_K1486040/annotation2387193/range2387193/1
component: BBa_K1486000

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type: Range
persistent identity: range2387193
display id: range2387193
version: 1
direction: direction/reverse
top level: BBa_K1486040
start: 1904
end: 2605
orientation: inline

BBa_K1486037
type: SequenceAnnotation
persistent identity: annotation2387189
display id: annotation2387189
version: 1
top level: BBa_K1486040
location: https://synbiohub.org/public/igem/BBa_K1486040/annotation2387189/range2387189/1
component: BBa_K1486037

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type: Range
persistent identity: range2387189
display id: range2387189
version: 1
direction: direction/reverse
top level: BBa_K1486040
start: 1865
end: 1903
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2387187
display id: annotation2387187
version: 1
top level: BBa_K1486040
location: https://synbiohub.org/public/igem/BBa_K1486040/annotation2387187/range2387187/1
component: BBa_B0034

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type: Range
persistent identity: range2387187
display id: range2387187
version: 1
direction: direction/reverse
top level: BBa_K1486040
start: 1211
end: 1222
orientation: inline

BBa_K1486038
type: SequenceAnnotation
persistent identity: annotation2387188
display id: annotation2387188
version: 1
top level: BBa_K1486040
location: https://synbiohub.org/public/igem/BBa_K1486040/annotation2387188/range2387188/1
component: BBa_K1486038

https://synbiohub.org/public/igem/BBa_K1486040/annotation2387188/range2387188/1
type: Range
persistent identity: range2387188
display id: range2387188
version: 1
direction: direction/reverse
top level: BBa_K1486040
start: 1223
end: 1864
orientation: inline

BBa_K1399015
type: ComponentDefinition
persistent identity: BBa K1399015
display id: BBa_K1399015
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Plac-GFP(LVA)
creator: Anna Stikane
created: 2014-09-18T11:00:00Z
modified: 2015-05-08T01:10:16Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1777_
m user id: 0
owner id: 22477
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: Lactose/IPTG inducible promoter with GFP reporter tagged with AAV-ssrA degradation tag followed by terminator. The tagged GFP is actively degraded within cell, thus provides better temporal resolution of green fluorescence and promoter activity.
mutable notes: Part was assembled using BrickClip assembly (BBF RFC104) using other biobrick parts as templates. BrickClip assembly is a special case of more general Paperclip assembly method.[1]
mutable provenance: other biobricks
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_B0015
component: BBa_K1399004
component: BBa_B0034
component: BBa_R0010
sequence annotation: BBa_K1399004
sequence annotation: BBa_B0034
sequence annotation: BBa_B0015
sequence annotation: BBa_R0010
sequence: https://synbiohub.org/public/igem/BBa_K1399015_sequence/1

BBa_B0015
type: Component
persistent identity: component2384085
display id: component2384085
version: 1
top level: BBa_K1399015
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_K1399004
type: Component
persistent identity: component2384078
display id: component2384078
version: 1
top level: BBa_K1399015
access: public
definition: BBa_K1399004

BBa_B0034
type: Component
persistent identity: component2384072
display id: component2384072
version: 1
top level: BBa_K1399015
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_R0010
type: Component
persistent identity: component2384064
display id: component2384064
version: 1
top level: BBa_K1399015
access: public
definition: https://synbiohub.org/public/igem/BBa_R0010/1

BBa_K1399004
type: SequenceAnnotation
persistent identity: annotation2384078
display id: annotation2384078
version: 1
top level: BBa_K1399015
location: https://synbiohub.org/public/igem/BBa_K1399015/annotation2384078/range2384078/1
component: BBa_K1399004

https://synbiohub.org/public/igem/BBa_K1399015/annotation2384078/range2384078/1
type: Range
persistent identity: range2384078
display id: range2384078
version: 1
direction: direction/reverse
top level: BBa_K1399015
start: 227
end: 979
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2384072
display id: annotation2384072
version: 1
top level: BBa_K1399015
location: https://synbiohub.org/public/igem/BBa_K1399015/annotation2384072/range2384072/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1399015/annotation2384072/range2384072/1
type: Range
persistent identity: range2384072
display id: range2384072
version: 1
direction: direction/reverse
top level: BBa_K1399015
start: 209
end: 220
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2384085
display id: annotation2384085
version: 1
top level: BBa_K1399015
location: https://synbiohub.org/public/igem/BBa_K1399015/annotation2384085/range2384085/1
component: BBa_B0015

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type: Range
persistent identity: range2384085
display id: range2384085
version: 1
direction: direction/reverse
top level: BBa_K1399015
start: 988
end: 1116
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation2384064
display id: annotation2384064
version: 1
top level: BBa_K1399015
location: https://synbiohub.org/public/igem/BBa_K1399015/annotation2384064/range2384064/1
component: BBa_R0010

https://synbiohub.org/public/igem/BBa_K1399015/annotation2384064/range2384064/1
type: Range
persistent identity: range2384064
display id: range2384064
version: 1
direction: direction/forward
top level: BBa_K1399015
start: 1
end: 200
orientation: inline

BBa_K1680024
type: ComponentDefinition
persistent identity: BBa K1680024
display id: BBa_K1680024
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Dronpa caged Cre with NLS
creator: Nicolai von Kuegelgen
created: 2015-09-16T11:00:00Z
modified: 2016-01-26T10:22:01Z
discontinued: false
dominant: false
group u list: _2098_
m user id: 4206
owner id: 26366
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Protein coding region for a fusion protein of Cre recombinase (BBa_K1680007) that is C- & N-terminal flanked with 145N Dronpa (BBa_K1680006) using a 15 AA linker as spacer (BBa_K1680003). The construct has a N-terminal NLS sequence (BBa_K1680004). In the expressed construct the two dronpa domains should form multimerize leading to an inactive Cre recombinase that is reversibly activatable through dronpa illumination (see Zhou et al 2012).
mutable notes: 3A assembly with RFC25
mutable provenance: gBlock design by Team Tuebingen
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: K1680007
sequence annotation: K1680003
sequence annotation: K1680006
sequence annotation: K1680004
sequence: https://synbiohub.org/public/igem/BBa_K1680024_sequence/1

K1680007
type: SequenceAnnotation
persistent identity: annotation2477392
display id: annotation2477392
version: 1
top level: BBa_K1680024
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1680024/annotation2477392/range2477392/1

https://synbiohub.org/public/igem/BBa_K1680024/annotation2477392/range2477392/1
type: Range
persistent identity: range2477392
display id: range2477392
version: 1
direction: direction/forward
top level: BBa_K1680024
start: 745
end: 1773
orientation: inline

K1680003
type: SequenceAnnotation
persistent identity: annotation2477395
display id: annotation2477395
version: 1
top level: BBa_K1680024
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1680024/annotation2477395/range2477395/1

https://synbiohub.org/public/igem/BBa_K1680024/annotation2477395/range2477395/1
type: Range
persistent identity: range2477395
display id: range2477395
version: 1
direction: direction/forward
top level: BBa_K1680024
start: 700
end: 744
orientation: inline

K1680006
type: SequenceAnnotation
persistent identity: annotation2477394
display id: annotation2477394
version: 1
top level: BBa_K1680024
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1680024/annotation2477394/range2477394/1

https://synbiohub.org/public/igem/BBa_K1680024/annotation2477394/range2477394/1
type: Range
persistent identity: range2477394
display id: range2477394
version: 1
direction: direction/forward
top level: BBa_K1680024
start: 1818
end: 2487
orientation: inline

K1680006
type: SequenceAnnotation
persistent identity: annotation2477393
display id: annotation2477393
version: 1
top level: BBa_K1680024
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1680024/annotation2477393/range2477393/1

https://synbiohub.org/public/igem/BBa_K1680024/annotation2477393/range2477393/1
type: Range
persistent identity: range2477393
display id: range2477393
version: 1
direction: direction/forward
top level: BBa_K1680024
start: 31
end: 699
orientation: inline

K1680003
type: SequenceAnnotation
persistent identity: annotation2477396
display id: annotation2477396
version: 1
top level: BBa_K1680024
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1680024/annotation2477396/range2477396/1

https://synbiohub.org/public/igem/BBa_K1680024/annotation2477396/range2477396/1
type: Range
persistent identity: range2477396
display id: range2477396
version: 1
direction: direction/forward
top level: BBa_K1680024
start: 1774
end: 1817
orientation: inline

K1680004
type: SequenceAnnotation
persistent identity: annotation2477390
display id: annotation2477390
version: 1
top level: BBa_K1680024
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1680024/annotation2477390/range2477390/1

https://synbiohub.org/public/igem/BBa_K1680024/annotation2477390/range2477390/1
type: Range
persistent identity: range2477390
display id: range2477390
version: 1
direction: direction/forward
top level: BBa_K1680024
start: 1
end: 24
orientation: inline

BBa_K1732022
type: ComponentDefinition
persistent identity: BBa K1732022
display id: BBa_K1732022
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: EGFPCO-His
creator: Donna Lee
created: 2015-09-17T11:00:00Z
modified: 2016-01-21T02:51:07Z
discontinued: false
dominant: false
group u list: _2154_
m user id: 4206
owner id: 27195
owning group id: 9
status: status/Planning
bookmark: false
mutable description: EGFP (BBa_1491002) with 6X-His tag for easier protein purification. Original sequence is codon optimized by IDT Codon Optimized Tool for E.coli.
mutable notes: Codon optimized for E.coli and His tag allows for easier protein purification.
mutable provenance: BBa_1491002
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: EGFPCO
sequence annotation: 6X-His
sequence: https://synbiohub.org/public/igem/BBa_K1732022_sequence/1

EGFPCO
type: SequenceAnnotation
persistent identity: annotation2469080
display id: annotation2469080
version: 1
top level: BBa_K1732022
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1732022/annotation2469080/range2469080/1

https://synbiohub.org/public/igem/BBa_K1732022/annotation2469080/range2469080/1
type: Range
persistent identity: range2469080
display id: range2469080
version: 1
direction: direction/forward
top level: BBa_K1732022
start: 1
end: 716
orientation: inline

6X-His
type: SequenceAnnotation
persistent identity: annotation2469121
display id: annotation2469121
version: 1
top level: BBa_K1732022
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1732022/annotation2469121/range2469121/1

https://synbiohub.org/public/igem/BBa_K1732022/annotation2469121/range2469121/1
type: Range
persistent identity: range2469121
display id: range2469121
version: 1
direction: direction/forward
top level: BBa_K1732022
start: 717
end: 733
orientation: inline

GFP_His
type: ComponentDefinition
persistent identity: BBa K863121
display id: BBa_K863121
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (His-tag)
creator: Moritz M??ller
created: 2012-09-19T11:00:00Z
modified: 2016-01-25T01:33:16Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1123_
m user id: 4206
owner id: 13080
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Untagged version of gfp from Repressilator reporter. See the [http://partsregistry.org/wiki/index.php?title=Part:BBa_E0040 untagged version] for source information.
mutable notes: Added Freiburg-restriction-sites for in frame assembly.
mutable provenance: [http://partsregistry.org/Part:BBa_I13522 BBa_I13522]
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: ATG
sequence annotation: NgoMIV
sequence annotation: AgeI
sequence annotation: His-Tag
sequence annotation: BBa_E0040
sequence annotation: TAA
sequence: https://synbiohub.org/public/igem/BBa_K863121_sequence/1

ATG
type: SequenceAnnotation
persistent identity: annotation2187748
display id: annotation2187748
version: 1
top level: GFP_His
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K863121/annotation2187748/range2187748/1

https://synbiohub.org/public/igem/BBa_K863121/annotation2187748/range2187748/1
type: Range
persistent identity: range2187748
display id: range2187748
version: 1
direction: direction/forward
top level: GFP_His
start: 1
end: 3
orientation: inline

NgoMIV
type: SequenceAnnotation
persistent identity: annotation2187747
display id: annotation2187747
version: 1
top level: GFP_His
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K863121/annotation2187747/range2187747/1

https://synbiohub.org/public/igem/BBa_K863121/annotation2187747/range2187747/1
type: Range
persistent identity: range2187747
display id: range2187747
version: 1
direction: direction/forward
top level: GFP_His
start: 4
end: 9
orientation: inline

AgeI
type: SequenceAnnotation
persistent identity: annotation2187756
display id: annotation2187756
version: 1
top level: GFP_His
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K863121/annotation2187756/range2187756/1

https://synbiohub.org/public/igem/BBa_K863121/annotation2187756/range2187756/1
type: Range
persistent identity: range2187756
display id: range2187756
version: 1
direction: direction/forward
top level: GFP_His
start: 742
end: 747
orientation: inline

His-Tag
type: SequenceAnnotation
persistent identity: annotation2187759
display id: annotation2187759
version: 1
top level: GFP_His
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K863121/annotation2187759/range2187759/1

https://synbiohub.org/public/igem/BBa_K863121/annotation2187759/range2187759/1
type: Range
persistent identity: range2187759
display id: range2187759
version: 1
direction: direction/forward
top level: GFP_His
start: 724
end: 741
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation2187765
display id: annotation2187765
version: 1
top level: GFP_His
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K863121/annotation2187765/range2187765/1

https://synbiohub.org/public/igem/BBa_K863121/annotation2187765/range2187765/1
type: Range
persistent identity: range2187765
display id: range2187765
version: 1
direction: direction/forward
top level: GFP_His
start: 10
end: 723
orientation: inline

TAA
type: SequenceAnnotation
persistent identity: annotation2187751
display id: annotation2187751
version: 1
top level: GFP_His
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K863121/annotation2187751/range2187751/1

https://synbiohub.org/public/igem/BBa_K863121/annotation2187751/range2187751/1
type: Range
persistent identity: range2187751
display id: range2187751
version: 1
direction: direction/forward
top level: GFP_His
start: 748
end: 750
orientation: inline

J23101::gf
type: ComponentDefinition
persistent identity: BBa K1620006
display id: BBa_K1620006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Constitutive GFP generator device
creator: Celio Dias Santos Jr
created: 2015-09-02T11:00:00Z
modified: 2015-09-18T03:02:02Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2037_
m user id: 23597
owner id: 23597
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: It generates permanently and a low rate GFP reporter. It is usefull for cell marker and promoter strength tests.
mutable notes: Assembly method used was 3A assembly.
mutable provenance: This part is assembled from parts BBa_J23101 and GFP generator (BBa_E0840).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_E0840
component: BBa_J23101
sequence annotation: BBa_E0840
sequence annotation: BBa_J23101
sequence: https://synbiohub.org/public/igem/BBa_K1620006_sequence/1

BBa_E0840
type: Component
persistent identity: component2443257
display id: component2443257
version: 1
top level: J23101::gf
access: public
definition: https://synbiohub.org/public/igem/BBa_E0840/1

BBa_J23101
type: Component
persistent identity: component2443246
display id: component2443246
version: 1
top level: J23101::gf
access: public
definition: https://synbiohub.org/public/igem/BBa_J23101/1

BBa_E0840
type: SequenceAnnotation
persistent identity: annotation2443257
display id: annotation2443257
version: 1
top level: J23101::gf
location: https://synbiohub.org/public/igem/BBa_K1620006/annotation2443257/range2443257/1
component: BBa_E0840

https://synbiohub.org/public/igem/BBa_K1620006/annotation2443257/range2443257/1
type: Range
persistent identity: range2443257
display id: range2443257
version: 1
direction: direction/reverse
top level: J23101::gf
start: 44
end: 921
orientation: inline

BBa_J23101
type: SequenceAnnotation
persistent identity: annotation2443246
display id: annotation2443246
version: 1
top level: J23101::gf
location: https://synbiohub.org/public/igem/BBa_K1620006/annotation2443246/range2443246/1
component: BBa_J23101

https://synbiohub.org/public/igem/BBa_K1620006/annotation2443246/range2443246/1
type: Range
persistent identity: range2443246
display id: range2443246
version: 1
direction: direction/reverse
top level: J23101::gf
start: 1
end: 35
orientation: inline

BBa_K1399004
type: ComponentDefinition
persistent identity: BBa K1399004
display id: BBa_K1399004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: GFP (mut3b) with LVA-ssrA degradation tag
creator: Anna Stikane
created: 2014-09-18T11:00:00Z
modified: 2015-06-01T07:07:29Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1777_
m user id: 4206
owner id: 22477
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: GFP (mut3b) (see part BBa_E0040) with added LVA-ssrA degradation tag. The tag increases GFP turn-over rate, thus providing better temporal resolution of green fluorescence. In the same time, maximal fluorescence amplitudes will be lower as newly formed protein is degraded as soon as it is formed. The tag encodes peptide sequence AANDENYALVA and is recognized by ClpA and ClpX unfoldases and ClpX mediator SspB.[1] ClpA and ClpX then form a proteosome-like complex with ClpP protease and the protein is degraded.[1] The final three residues of the tag determines the strength of interaction with ClpX and thus the final protein degradation rate.[2] The LVA tag is reported to lead to fast protein degradation, degrading GFP with rate -0.018 per minute.[2] However, be aware that exact protein degradation rate depends on multiple factors: ClpXP and ClpAP protease and SspB mediator concentrations, protein stability, Km of binding to the protease, temperature [3]. References [1] Flynn, J. M. et al. Overlapping recognition determinants within the ssrA degradation tag allow modulation of proteolysis. Proc. Natl. Acad. Sci. U. S. A. 98, 10584???9 (2001). [2] Andersen, J. B. et al. New unstable variants of green fluorescent protein for studies of transient gene expression in bacteria. Appl. Environ. Microbiol. 64, 2240???6 (1998). [3] Purcell, O., Grierson, C. S., Bernardo, M. Di & Savery, N. J. Temperature dependence of ssrA-tag mediated protein degradation. J. Biol. Eng. 6, 10 (2012).
mutable notes: The tag was attached to GFP using PCR and MABEL (mutagenesis with blunt-end ligation), thus avoiding introduction of additonal residues and restriction site. Different parts of the tag are recognized by different proteins, for example, the final 3 residues (LVA in this case) are recognised by ClpX, whereas first 4 residues of the tag are required for efficient SspB binding.[1] Thus modifications of these critical residues alter the efficacy with what different proteases bind to it.
mutable provenance: GFP comes from part E1010, the tag sequence was obtained from paper by Andersen et al., (1998).[2]
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: GFP (mut3b)
sequence annotation: stop
sequence annotation: start
sequence annotation: LVA
sequence: https://synbiohub.org/public/igem/BBa_K1399004_sequence/1

GFP (mut3b)
type: SequenceAnnotation
persistent identity: annotation2383914
display id: annotation2383914
version: 1
top level: BBa_K1399004
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1399004/annotation2383914/range2383914/1

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display id: range2383914
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stop
type: SequenceAnnotation
persistent identity: annotation2383916
display id: annotation2383916
version: 1
top level: BBa_K1399004
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1399004/annotation2383916/range2383916/1

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persistent identity: range2383916
display id: range2383916
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end: 750
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stop
type: SequenceAnnotation
persistent identity: annotation2383917
display id: annotation2383917
version: 1
top level: BBa_K1399004
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1399004/annotation2383917/range2383917/1

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display id: range2383917
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start
type: SequenceAnnotation
persistent identity: annotation2383913
display id: annotation2383913
version: 1
top level: BBa_K1399004
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1399004/annotation2383913/range2383913/1

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type: Range
persistent identity: range2383913
display id: range2383913
version: 1
direction: direction/forward
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start: 1
end: 3
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LVA
type: SequenceAnnotation
persistent identity: annotation2383915
display id: annotation2383915
version: 1
top level: BBa_K1399004
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1399004/annotation2383915/range2383915/1

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display id: range2383915
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start: 715
end: 747
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BBa_K1321346
type: ComponentDefinition
persistent identity: BBa K1321346
display id: BBa_K1321346
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CBDclos fused to sfGFP in RFC 25
creator: Chris N Micklem
created: 2014-10-07T11:00:00Z
modified: 2015-06-17T12:30:31Z
discontinued: false
dominant: false
group u list: _1696_
m user id: 4206
owner id: 20830
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: asdf
mutable notes: asdf
mutable provenance: asdf
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_K1321002
component: BBa_B0105
component: BBa_K1321337
sequence annotation: BBa_K1321337
sequence annotation: BBa_B0105
sequence annotation: BBa_K1321002
sequence: https://synbiohub.org/public/igem/BBa_K1321346_sequence/1

BBa_K1321002
type: Component
persistent identity: component2415121
display id: component2415121
version: 1
top level: BBa_K1321346
access: public
definition: https://synbiohub.org/public/igem/BBa_K1321002/1

BBa_B0105
type: Component
persistent identity: component2415123
display id: component2415123
version: 1
top level: BBa_K1321346
access: public
definition: https://synbiohub.org/public/igem/BBa_B0105/1

BBa_K1321337
type: Component
persistent identity: component2415125
display id: component2415125
version: 1
top level: BBa_K1321346
access: public
definition: BBa_K1321337

BBa_K1321337
type: SequenceAnnotation
persistent identity: annotation2415125
display id: annotation2415125
version: 1
top level: BBa_K1321346
location: https://synbiohub.org/public/igem/BBa_K1321346/annotation2415125/range2415125/1
component: BBa_K1321337

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display id: range2415125
version: 1
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end: 1017
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BBa_B0105
type: SequenceAnnotation
persistent identity: annotation2415123
display id: annotation2415123
version: 1
top level: BBa_K1321346
location: https://synbiohub.org/public/igem/BBa_K1321346/annotation2415123/range2415123/1
component: BBa_B0105

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type: Range
persistent identity: range2415123
display id: range2415123
version: 1
direction: direction/reverse
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start: 301
end: 306
orientation: inline

BBa_K1321002
type: SequenceAnnotation
persistent identity: annotation2415121
display id: annotation2415121
version: 1
top level: BBa_K1321346
location: https://synbiohub.org/public/igem/BBa_K1321346/annotation2415121/range2415121/1
component: BBa_K1321002

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type: Range
persistent identity: range2415121
display id: range2415121
version: 1
direction: direction/reverse
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start: 1
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BBa_K1486005
type: ComponentDefinition
persistent identity: BBa K1486005
display id: BBa_K1486005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Arabinose promoter + sfGFP-CpxR [Cterm]
creator: iGem EPFL 2014
created: 2014-08-12T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part is composed of an arabinose-inducible promoter for superfolded GFP attached by a linker to CpxR (on the C terminus of CpxR).
mutable notes: We had to test both attachments, N and C terminus of the CpxR.
mutable provenance: The arabinose promoter and the sfGFP were obtained from the registry, while the CpxR was obtained from the E coli genome.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K1486003
component: BBa_K1486000
component: BBa_B0034
component: BBa_I0500
component: BBa_I746916
sequence annotation: BBa_B0034
sequence annotation: BBa_I746916
sequence annotation: BBa_I0500
sequence annotation: BBa_K1486000
sequence annotation: BBa_K1486003
sequence: https://synbiohub.org/public/igem/BBa_K1486005_sequence/1

BBa_K1486003
type: Component
persistent identity: component2382089
display id: component2382089
version: 1
top level: BBa_K1486005
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486003/1

BBa_K1486000
type: Component
persistent identity: component2382088
display id: component2382088
version: 1
top level: BBa_K1486005
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486000/1

BBa_B0034
type: Component
persistent identity: component2382084
display id: component2382084
version: 1
top level: BBa_K1486005
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_I0500
type: Component
persistent identity: component2382082
display id: component2382082
version: 1
top level: BBa_K1486005
access: public
definition: https://synbiohub.org/public/igem/BBa_I0500/1

BBa_I746916
type: Component
persistent identity: component2382093
display id: component2382093
version: 1
top level: BBa_K1486005
access: public
definition: https://synbiohub.org/public/igem/BBa_I746916/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2382084
display id: annotation2382084
version: 1
top level: BBa_K1486005
location: https://synbiohub.org/public/igem/BBa_K1486005/annotation2382084/range2382084/1
component: BBa_B0034

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type: Range
persistent identity: range2382084
display id: range2382084
version: 1
direction: direction/reverse
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start: 1211
end: 1222
orientation: inline

BBa_I746916
type: SequenceAnnotation
persistent identity: annotation2382093
display id: annotation2382093
version: 1
top level: BBa_K1486005
location: https://synbiohub.org/public/igem/BBa_K1486005/annotation2382093/range2382093/1
component: BBa_I746916

https://synbiohub.org/public/igem/BBa_K1486005/annotation2382093/range2382093/1
type: Range
persistent identity: range2382093
display id: range2382093
version: 1
direction: direction/reverse
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start: 1949
end: 2668
orientation: inline

BBa_I0500
type: SequenceAnnotation
persistent identity: annotation2382082
display id: annotation2382082
version: 1
top level: BBa_K1486005
location: https://synbiohub.org/public/igem/BBa_K1486005/annotation2382082/range2382082/1
component: BBa_I0500

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type: Range
persistent identity: range2382082
display id: range2382082
version: 1
direction: direction/reverse
top level: BBa_K1486005
start: 1
end: 1210
orientation: inline

BBa_K1486000
type: SequenceAnnotation
persistent identity: annotation2382088
display id: annotation2382088
version: 1
top level: BBa_K1486005
location: https://synbiohub.org/public/igem/BBa_K1486005/annotation2382088/range2382088/1
component: BBa_K1486000

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type: Range
persistent identity: range2382088
display id: range2382088
version: 1
direction: direction/reverse
top level: BBa_K1486005
start: 1223
end: 1924
orientation: inline

BBa_K1486003
type: SequenceAnnotation
persistent identity: annotation2382089
display id: annotation2382089
version: 1
top level: BBa_K1486005
location: https://synbiohub.org/public/igem/BBa_K1486005/annotation2382089/range2382089/1
component: BBa_K1486003

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type: Range
persistent identity: range2382089
display id: range2382089
version: 1
direction: direction/reverse
top level: BBa_K1486005
start: 1925
end: 1948
orientation: inline

BBa_K1486039
type: ComponentDefinition
persistent identity: BBa K1486039
display id: BBa_K1486039
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: sfGFPC
creator: EPFL iGem team 2014
created: 2014-09-26T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: To edit
mutable notes: To edit
mutable provenance: To edit
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence: https://synbiohub.org/public/igem/BBa_K1486039_sequence/1

BBa_K082003
type: ComponentDefinition
persistent identity: BBa K082003
display id: BBa_K082003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: GFP(+LVA)
creator: Yanting Xue , He Shen
created: 2008-10-27T12:00:00Z
modified: 2016-01-25T04:43:13Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _228_
m user id: 4206
owner id: 1560
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: noen
mutable notes: none
mutable provenance: none
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: GFP
sequence annotation: LVA
sequence: https://synbiohub.org/public/igem/BBa_K082003_sequence/1

GFP
type: SequenceAnnotation
persistent identity: annotation1992959
display id: annotation1992959
version: 1
top level: BBa_K082003
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K082003/annotation1992959/range1992959/1

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type: Range
persistent identity: range1992959
display id: range1992959
version: 1
direction: direction/forward
top level: BBa_K082003
start: 1
end: 720
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation1993327
display id: annotation1993327
version: 1
top level: BBa_K082003
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K082003/annotation1993327/range1993327/1

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type: Range
persistent identity: range1993327
display id: range1993327
version: 1
direction: direction/forward
top level: BBa_K082003
start: 721
end: 753
orientation: inline

BBa_K106671
type: ComponentDefinition
persistent identity: BBa K106671
display id: BBa_K106671
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: GFP, Aar1 AD part
creator: Jacinto Chen
created: 2008-10-28T12:00:00Z
modified: 2016-01-26T09:50:57Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _226_
m user id: 4206
owner id: 3418
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This is the coding sequence of a yeast optimized GFP with BD ends for AarI cloning.
mutable notes: As an AD part, there is no ATG, and the stop codon is included. For more information on AarI cloning, please refer to the UCSF 2008 igem page.
mutable provenance: PCR amplified from lab plasmid stocks.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: BBa_K106671
sequence: https://synbiohub.org/public/igem/BBa_K106671_sequence/1

BBa_K106671
type: SequenceAnnotation
persistent identity: annotation1993686
display id: annotation1993686
version: 1
top level: BBa_K106671
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K106671/annotation1993686/range1993686/1

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type: Range
persistent identity: range1993686
display id: range1993686
version: 1
direction: direction/reverse
top level: BBa_K106671
start: 1
end: 714
orientation: inline

BBa_K1399014
type: ComponentDefinition
persistent identity: BBa K1399014
display id: BBa_K1399014
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Plac-GFP(AAV)
creator: Anna Stikane
created: 2014-09-18T11:00:00Z
modified: 2015-05-08T01:10:16Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1777_
m user id: 0
owner id: 22477
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: Lactose/IPTG inducible promoter with GFP reporter tagged with AAV-ssrA degradation tag followed by terminator. The tagged GFP is actively degraded within cell, thus provides better temporal resolution of green fluorescence and promoter activity.
mutable notes: Part was assembled using BrickClip assembly (BBF RFC104) using other biobrick parts as templates. BrickClip assembly is a special case of more general Paperclip assembly method.[1]
mutable provenance: other biobricks
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_K1399005
component: BBa_B0015
component: BBa_R0010
component: BBa_B0034
sequence annotation: BBa_K1399005
sequence annotation: BBa_B0034
sequence annotation: BBa_B0015
sequence annotation: BBa_R0010
sequence: https://synbiohub.org/public/igem/BBa_K1399014_sequence/1

BBa_K1399005
type: Component
persistent identity: component2384055
display id: component2384055
version: 1
top level: BBa_K1399014
access: public
definition: https://synbiohub.org/public/igem/BBa_K1399005/1

BBa_B0015
type: Component
persistent identity: component2384062
display id: component2384062
version: 1
top level: BBa_K1399014
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_R0010
type: Component
persistent identity: component2384041
display id: component2384041
version: 1
top level: BBa_K1399014
access: public
definition: https://synbiohub.org/public/igem/BBa_R0010/1

BBa_B0034
type: Component
persistent identity: component2384049
display id: component2384049
version: 1
top level: BBa_K1399014
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K1399005
type: SequenceAnnotation
persistent identity: annotation2384055
display id: annotation2384055
version: 1
top level: BBa_K1399014
location: https://synbiohub.org/public/igem/BBa_K1399014/annotation2384055/range2384055/1
component: BBa_K1399005

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type: Range
persistent identity: range2384055
display id: range2384055
version: 1
direction: direction/reverse
top level: BBa_K1399014
start: 227
end: 979
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2384049
display id: annotation2384049
version: 1
top level: BBa_K1399014
location: https://synbiohub.org/public/igem/BBa_K1399014/annotation2384049/range2384049/1
component: BBa_B0034

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type: Range
persistent identity: range2384049
display id: range2384049
version: 1
direction: direction/reverse
top level: BBa_K1399014
start: 209
end: 220
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2384062
display id: annotation2384062
version: 1
top level: BBa_K1399014
location: https://synbiohub.org/public/igem/BBa_K1399014/annotation2384062/range2384062/1
component: BBa_B0015

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type: Range
persistent identity: range2384062
display id: range2384062
version: 1
direction: direction/reverse
top level: BBa_K1399014
start: 988
end: 1116
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation2384041
display id: annotation2384041
version: 1
top level: BBa_K1399014
location: https://synbiohub.org/public/igem/BBa_K1399014/annotation2384041/range2384041/1
component: BBa_R0010

https://synbiohub.org/public/igem/BBa_K1399014/annotation2384041/range2384041/1
type: Range
persistent identity: range2384041
display id: range2384041
version: 1
direction: direction/forward
top level: BBa_K1399014
start: 1
end: 200
orientation: inline

BBa_K1486034
type: ComponentDefinition
persistent identity: BBa K1486034
display id: BBa_K1486034
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Yeast optimized superfolder GFP C-terminus (215-238)
creator: EPFL iGem team 2014
created: 2014-09-26T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: To edit
mutable notes: To edit
mutable provenance: To edit
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000324
role: partType/Tag
sequence annotation: Yeast optimized sfGFP C-terminus
sequence: https://synbiohub.org/public/igem/BBa_K1486034_sequence/1

Yeast optimized sfGFP C-terminus
type: SequenceAnnotation
persistent identity: annotation2417784
display id: annotation2417784
version: 1
top level: BBa_K1486034
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1486034/annotation2417784/range2417784/1

https://synbiohub.org/public/igem/BBa_K1486034/annotation2417784/range2417784/1
type: Range
persistent identity: range2417784
display id: range2417784
version: 1
direction: direction/forward
top level: BBa_K1486034
start: 1
end: 75
orientation: inline

pUSPA::gfp
type: ComponentDefinition
persistent identity: BBa K1620005
display id: BBa_K1620005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: GFP device responsive to environmental stresses
creator: Celio Dias Santos Jr
created: 2015-09-02T11:00:00Z
modified: 2015-09-13T03:59:02Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2037_
m user id: 23597
owner id: 23597
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This device takes environmental stresses as input a transcriptional signal (PoPS) and produce as output the fluorescent protein GFP.
mutable notes: This part was assembled using 3A assembly method.
mutable provenance: This part is an assembly of promoter uspA (BBa_K1620000) and GFP generator device (BBa_E0840).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_K1620000
component: BBa_E0840
sequence annotation: BBa_K1620000
sequence annotation: BBa_E0840
sequence: https://synbiohub.org/public/igem/BBa_K1620005_sequence/1

BBa_K1620000
type: Component
persistent identity: component2443234
display id: component2443234
version: 1
top level: pUSPA::gfp
access: public
definition: https://synbiohub.org/public/igem/BBa_K1620000/1

BBa_E0840
type: Component
persistent identity: component2443245
display id: component2443245
version: 1
top level: pUSPA::gfp
access: public
definition: https://synbiohub.org/public/igem/BBa_E0840/1

BBa_K1620000
type: SequenceAnnotation
persistent identity: annotation2443234
display id: annotation2443234
version: 1
top level: pUSPA::gfp
location: https://synbiohub.org/public/igem/BBa_K1620005/annotation2443234/range2443234/1
component: BBa_K1620000

https://synbiohub.org/public/igem/BBa_K1620005/annotation2443234/range2443234/1
type: Range
persistent identity: range2443234
display id: range2443234
version: 1
direction: direction/reverse
top level: pUSPA::gfp
start: 1
end: 46
orientation: inline

BBa_E0840
type: SequenceAnnotation
persistent identity: annotation2443245
display id: annotation2443245
version: 1
top level: pUSPA::gfp
location: https://synbiohub.org/public/igem/BBa_K1620005/annotation2443245/range2443245/1
component: BBa_E0840

https://synbiohub.org/public/igem/BBa_K1620005/annotation2443245/range2443245/1
type: Range
persistent identity: range2443245
display id: range2443245
version: 1
direction: direction/reverse
top level: pUSPA::gfp
start: 55
end: 932
orientation: inline

BBa_K1980010
type: ComponentDefinition
persistent identity: BBa K1980010
display id: BBa_K1980010
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pCopA TAT Csp1 sfGFP with divergent CueR
creator: Sam Garforth
created: 2016-10-10T11:00:00Z
modified: 2016-10-24T08:12:51Z
discontinued: false
dominant: false
experience: experience/Issues
group u list: _2447_
m user id: 29607
owner id: 29607
owning group id: 9
status: status/Planning
bookmark: false
mutable description: pCopA TAT Csp1 sfGFP with plasmid constitutively expressed CueR
mutable notes: aahahahah
mutable provenance: E. coli, methanoba trcol OB3b
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Other
role: SO:0000110
component: BBa_K1980001
component: BBa_K1980006
sequence annotation: BBa_K1980006
sequence annotation: BBa_K1980001
sequence: https://synbiohub.org/public/igem/BBa_K1980010_sequence/1

BBa_K1980001
type: Component
persistent identity: component2493042
display id: component2493042
version: 1
top level: BBa_K1980010
access: public
definition: Csp1sfGFP

BBa_K1980006
type: Component
persistent identity: component2493041
display id: component2493041
version: 1
top level: BBa_K1980010
access: public
definition: https://synbiohub.org/public/igem/BBa_K1980006/1

BBa_K1980006
type: SequenceAnnotation
persistent identity: annotation2493041
display id: annotation2493041
version: 1
top level: BBa_K1980010
location: https://synbiohub.org/public/igem/BBa_K1980010/annotation2493041/range2493041/1
component: BBa_K1980006

https://synbiohub.org/public/igem/BBa_K1980010/annotation2493041/range2493041/1
type: Range
persistent identity: range2493041
display id: range2493041
version: 1
direction: direction/reverse
top level: BBa_K1980010
start: 1
end: 574
orientation: inline

BBa_K1980001
type: SequenceAnnotation
persistent identity: annotation2493042
display id: annotation2493042
version: 1
top level: BBa_K1980010
location: https://synbiohub.org/public/igem/BBa_K1980010/annotation2493042/range2493042/1
component: BBa_K1980001

https://synbiohub.org/public/igem/BBa_K1980010/annotation2493042/range2493042/1
type: Range
persistent identity: range2493042
display id: range2493042
version: 1
direction: direction/reverse
top level: BBa_K1980010
start: 581
end: 1783
orientation: inline

CheZ-GFP
type: ComponentDefinition
persistent identity: BBa K1709002
display id: BBa_K1709002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CheZ-GFP
creator: Astrid Deryckere
created: 2015-09-09T11:00:00Z
modified: 2015-09-18T09:28:45Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2129_
m user id: 25389
owner id: 25389
owning group id: 9
status: status/Planning
bookmark: true
mutable description: Upon recognition of a small molecule (e.g. nutrients or toxins), chemoreceptors transduce a signal through a set of Che proteins. This group of methylesterases and phosphatases regulates the rotation of the flagella. At the end of the chain of Che proteins is CheY. In phosphorylated state, CheY binds the flagella and causes the cells to tumble. The phosphatase CheZ dephosphorylates CheY, inducing dissociation of CheY from the flagella enabling the cells to swim. Cells lacking the CheZ protein are unable to swim and will tumble excessively and incessantly.
mutable notes: 1 bp in BBa_K629003 was adapted to create an original restriciton site in the plasmid. CheZ was fused to GFP (BBa_K082003) and its LVA tag was codon-optimized to avoid introducing artificial DNA repetitions.
mutable provenance: DNA sequences originate from the E. Coli genome and previous biobrick sequences.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: CheZ Coding Region
sequence annotation: Ochre Stop Codon
sequence annotation: LVA-tag
sequence annotation: GFP Coding Region
sequence annotation: ATG Start Codon
sequence: https://synbiohub.org/public/igem/BBa_K1709002_sequence/1

CheZ Coding Region
type: SequenceAnnotation
persistent identity: annotation2448532
display id: annotation2448532
version: 1
top level: CheZ-GFP
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1709002/annotation2448532/range2448532/1

https://synbiohub.org/public/igem/BBa_K1709002/annotation2448532/range2448532/1
type: Range
persistent identity: range2448532
display id: range2448532
version: 1
direction: direction/forward
top level: CheZ-GFP
start: 1
end: 642
orientation: inline

Ochre Stop Codon
type: SequenceAnnotation
persistent identity: annotation2448531
display id: annotation2448531
version: 1
top level: CheZ-GFP
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1709002/annotation2448531/range2448531/1

https://synbiohub.org/public/igem/BBa_K1709002/annotation2448531/range2448531/1
type: Range
persistent identity: range2448531
display id: range2448531
version: 1
direction: direction/forward
top level: CheZ-GFP
start: 1399
end: 1401
orientation: inline

LVA-tag
type: SequenceAnnotation
persistent identity: annotation2448534
display id: annotation2448534
version: 1
top level: CheZ-GFP
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1709002/annotation2448534/range2448534/1

https://synbiohub.org/public/igem/BBa_K1709002/annotation2448534/range2448534/1
type: Range
persistent identity: range2448534
display id: range2448534
version: 1
direction: direction/forward
top level: CheZ-GFP
start: 1366
end: 1401
orientation: inline

GFP Coding Region
type: SequenceAnnotation
persistent identity: annotation2448533
display id: annotation2448533
version: 1
top level: CheZ-GFP
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1709002/annotation2448533/range2448533/1

https://synbiohub.org/public/igem/BBa_K1709002/annotation2448533/range2448533/1
type: Range
persistent identity: range2448533
display id: range2448533
version: 1
direction: direction/forward
top level: CheZ-GFP
start: 646
end: 1365
orientation: inline

ATG Start Codon
type: SequenceAnnotation
persistent identity: annotation2448530
display id: annotation2448530
version: 1
top level: CheZ-GFP
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1709002/annotation2448530/range2448530/1

https://synbiohub.org/public/igem/BBa_K1709002/annotation2448530/range2448530/1
type: Range
persistent identity: range2448530
display id: range2448530
version: 1
direction: direction/forward
top level: CheZ-GFP
start: 1
end: 3
orientation: inline

BBa_K1399023
type: ComponentDefinition
persistent identity: BBa K1399023
display id: BBa_K1399023
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Ptet-GFP(DAS)
creator: Anna Stikane
created: 2014-09-18T11:00:00Z
modified: 2015-05-08T01:10:16Z
discontinued: false
dominant: false
group u list: _1777_
m user id: 0
owner id: 22477
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: Tetracycline/aTc inducible promoter with GFP reporter tagged with DAS-ssrA degradation tag followed by terminator. The tagged GFP is actively degraded within cell, thus provides better temporal resolution of green fluorescence and promoter activity.Presence of SspB is crucial for effective protein degradation.
mutable notes: Part was assembled using BrickClip assembly (BBF RFC104) using other biobrick parts as templates. BrickClip assembly is a special case of more general Paperclip assembly method.[1]
mutable provenance: other biobricks
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_B0034
component: BBa_B0015
component: BBa_R0040
component: BBa_K1399008
sequence annotation: BBa_B0034
sequence annotation: BBa_K1399008
sequence annotation: BBa_B0015
sequence annotation: BBa_R0040
sequence: https://synbiohub.org/public/igem/BBa_K1399023_sequence/1

BBa_B0034
type: Component
persistent identity: component2384305
display id: component2384305
version: 1
top level: BBa_K1399023
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0015
type: Component
persistent identity: component2384318
display id: component2384318
version: 1
top level: BBa_K1399023
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_R0040
type: Component
persistent identity: component2384299
display id: component2384299
version: 1
top level: BBa_K1399023
access: public
definition: https://synbiohub.org/public/igem/BBa_R0040/1

BBa_K1399008
type: Component
persistent identity: component2384311
display id: component2384311
version: 1
top level: BBa_K1399023
access: public
definition: https://synbiohub.org/public/igem/BBa_K1399008/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2384305
display id: annotation2384305
version: 1
top level: BBa_K1399023
location: https://synbiohub.org/public/igem/BBa_K1399023/annotation2384305/range2384305/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1399023/annotation2384305/range2384305/1
type: Range
persistent identity: range2384305
display id: range2384305
version: 1
direction: direction/reverse
top level: BBa_K1399023
start: 63
end: 74
orientation: inline

BBa_K1399008
type: SequenceAnnotation
persistent identity: annotation2384311
display id: annotation2384311
version: 1
top level: BBa_K1399023
location: https://synbiohub.org/public/igem/BBa_K1399023/annotation2384311/range2384311/1
component: BBa_K1399008

https://synbiohub.org/public/igem/BBa_K1399023/annotation2384311/range2384311/1
type: Range
persistent identity: range2384311
display id: range2384311
version: 1
direction: direction/reverse
top level: BBa_K1399023
start: 81
end: 833
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2384318
display id: annotation2384318
version: 1
top level: BBa_K1399023
location: https://synbiohub.org/public/igem/BBa_K1399023/annotation2384318/range2384318/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K1399023/annotation2384318/range2384318/1
type: Range
persistent identity: range2384318
display id: range2384318
version: 1
direction: direction/reverse
top level: BBa_K1399023
start: 842
end: 970
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation2384299
display id: annotation2384299
version: 1
top level: BBa_K1399023
location: https://synbiohub.org/public/igem/BBa_K1399023/annotation2384299/range2384299/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_K1399023/annotation2384299/range2384299/1
type: Range
persistent identity: range2384299
display id: range2384299
version: 1
direction: direction/forward
top level: BBa_K1399023
start: 1
end: 54
orientation: inline

BBa_K1321349
type: ComponentDefinition
persistent identity: BBa K1321349
display id: BBa_K1321349
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CBDcex fused to sfGFP in RFC 25
creator: Chris N Micklem
created: 2014-10-07T11:00:00Z
modified: 2015-06-17T12:30:55Z
discontinued: false
dominant: false
group u list: _1696_
m user id: 4206
owner id: 20830
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: asdf
mutable notes: asdf
mutable provenance: asdf
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_B0105
component: BBa_K1321337
component: BBa_K1321003
sequence annotation: BBa_B0105
sequence annotation: BBa_K1321337
sequence annotation: BBa_K1321003
sequence: https://synbiohub.org/public/igem/BBa_K1321349_sequence/1

BBa_B0105
type: Component
persistent identity: component2415151
display id: component2415151
version: 1
top level: BBa_K1321349
access: public
definition: https://synbiohub.org/public/igem/BBa_B0105/1

BBa_K1321337
type: Component
persistent identity: component2415153
display id: component2415153
version: 1
top level: BBa_K1321349
access: public
definition: BBa_K1321337

BBa_K1321003
type: Component
persistent identity: component2415149
display id: component2415149
version: 1
top level: BBa_K1321349
access: public
definition: https://synbiohub.org/public/igem/BBa_K1321003/1

BBa_B0105
type: SequenceAnnotation
persistent identity: annotation2415151
display id: annotation2415151
version: 1
top level: BBa_K1321349
location: https://synbiohub.org/public/igem/BBa_K1321349/annotation2415151/range2415151/1
component: BBa_B0105

https://synbiohub.org/public/igem/BBa_K1321349/annotation2415151/range2415151/1
type: Range
persistent identity: range2415151
display id: range2415151
version: 1
direction: direction/reverse
top level: BBa_K1321349
start: 328
end: 333
orientation: inline

BBa_K1321337
type: SequenceAnnotation
persistent identity: annotation2415153
display id: annotation2415153
version: 1
top level: BBa_K1321349
location: https://synbiohub.org/public/igem/BBa_K1321349/annotation2415153/range2415153/1
component: BBa_K1321337

https://synbiohub.org/public/igem/BBa_K1321349/annotation2415153/range2415153/1
type: Range
persistent identity: range2415153
display id: range2415153
version: 1
direction: direction/reverse
top level: BBa_K1321349
start: 334
end: 1044
orientation: inline

BBa_K1321003
type: SequenceAnnotation
persistent identity: annotation2415149
display id: annotation2415149
version: 1
top level: BBa_K1321349
location: https://synbiohub.org/public/igem/BBa_K1321349/annotation2415149/range2415149/1
component: BBa_K1321003

https://synbiohub.org/public/igem/BBa_K1321349/annotation2415149/range2415149/1
type: Range
persistent identity: range2415149
display id: range2415149
version: 1
direction: direction/reverse
top level: BBa_K1321349
start: 1
end: 327
orientation: inline

BBa_K1399020
type: ComponentDefinition
persistent identity: BBa K1399020
display id: BBa_K1399020
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Ptet-GFP(LVA)
creator: Anna Stikane
created: 2014-09-18T11:00:00Z
modified: 2015-05-08T01:10:16Z
discontinued: false
dominant: false
group u list: _1777_
m user id: 0
owner id: 22477
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Tetracycline/aTc inducible promoter with GFP reporter tagged with LVA-ssrA degradation tag followed by terminator. The tagged GFP is actively degraded within cell, thus provides better temporal resolution of green fluorescence and promoter activity.
mutable notes: Part was assembled using BrickClip assembly (BBF RFC104) using other biobrick parts as templates. BrickClip assembly is a special case of more general Paperclip assembly method.[1]
mutable provenance: other biobricks
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_R0040
component: BBa_B0034
component: BBa_B0015
component: BBa_K1399004
sequence annotation: BBa_K1399004
sequence annotation: BBa_B0015
sequence annotation: BBa_B0034
sequence annotation: BBa_R0040
sequence: https://synbiohub.org/public/igem/BBa_K1399020_sequence/1

BBa_R0040
type: Component
persistent identity: component2384239
display id: component2384239
version: 1
top level: BBa_K1399020
access: public
definition: https://synbiohub.org/public/igem/BBa_R0040/1

BBa_B0034
type: Component
persistent identity: component2384245
display id: component2384245
version: 1
top level: BBa_K1399020
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0015
type: Component
persistent identity: component2384258
display id: component2384258
version: 1
top level: BBa_K1399020
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_K1399004
type: Component
persistent identity: component2384251
display id: component2384251
version: 1
top level: BBa_K1399020
access: public
definition: BBa_K1399004

BBa_K1399004
type: SequenceAnnotation
persistent identity: annotation2384251
display id: annotation2384251
version: 1
top level: BBa_K1399020
location: https://synbiohub.org/public/igem/BBa_K1399020/annotation2384251/range2384251/1
component: BBa_K1399004

https://synbiohub.org/public/igem/BBa_K1399020/annotation2384251/range2384251/1
type: Range
persistent identity: range2384251
display id: range2384251
version: 1
direction: direction/reverse
top level: BBa_K1399020
start: 81
end: 833
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2384258
display id: annotation2384258
version: 1
top level: BBa_K1399020
location: https://synbiohub.org/public/igem/BBa_K1399020/annotation2384258/range2384258/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K1399020/annotation2384258/range2384258/1
type: Range
persistent identity: range2384258
display id: range2384258
version: 1
direction: direction/reverse
top level: BBa_K1399020
start: 842
end: 970
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2384245
display id: annotation2384245
version: 1
top level: BBa_K1399020
location: https://synbiohub.org/public/igem/BBa_K1399020/annotation2384245/range2384245/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1399020/annotation2384245/range2384245/1
type: Range
persistent identity: range2384245
display id: range2384245
version: 1
direction: direction/reverse
top level: BBa_K1399020
start: 63
end: 74
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation2384239
display id: annotation2384239
version: 1
top level: BBa_K1399020
location: https://synbiohub.org/public/igem/BBa_K1399020/annotation2384239/range2384239/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_K1399020/annotation2384239/range2384239/1
type: Range
persistent identity: range2384239
display id: range2384239
version: 1
direction: direction/forward
top level: BBa_K1399020
start: 1
end: 54
orientation: inline

BBa_K1732020
type: ComponentDefinition
persistent identity: BBa K1732020
display id: BBa_K1732020
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: GFP-HIS
creator: Donna Lee
created: 2015-09-15T11:00:00Z
modified: 2016-01-25T01:12:20Z
discontinued: false
dominant: false
group u list: _2154_
m user id: 4206
owner id: 27195
owning group id: 9
status: status/Planning
bookmark: false
mutable description: GFP with a 6X His tag for easier protein purification.
mutable notes: Codon optimized for E.coli.
mutable provenance: Unknown
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: 6x-His
sequence annotation: GFPCO
sequence: https://synbiohub.org/public/igem/BBa_K1732020_sequence/1

6x-His
type: SequenceAnnotation
persistent identity: annotation2462825
display id: annotation2462825
version: 1
top level: BBa_K1732020
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1732020/annotation2462825/range2462825/1

https://synbiohub.org/public/igem/BBa_K1732020/annotation2462825/range2462825/1
type: Range
persistent identity: range2462825
display id: range2462825
version: 1
direction: direction/forward
top level: BBa_K1732020
start: 714
end: 731
orientation: inline

GFPCO
type: SequenceAnnotation
persistent identity: annotation2462824
display id: annotation2462824
version: 1
top level: BBa_K1732020
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1732020/annotation2462824/range2462824/1

https://synbiohub.org/public/igem/BBa_K1732020/annotation2462824/range2462824/1
type: Range
persistent identity: range2462824
display id: range2462824
version: 1
direction: direction/forward
top level: BBa_K1732020
start: 1
end: 713
orientation: inline

HisTag-GFP
type: ComponentDefinition
persistent identity: BBa K1602021
display id: BBa_K1602021
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Green fluorescent protein (GFP) with N-terminal His-Tag
creator: Jasmin Weber, Michelle Zoeller, Marcus Geissler, Stefan Bohn, Simon Linder, Sebastian Barthel
created: 2015-09-15T11:00:00Z
modified: 2016-01-25T01:10:25Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2019_
m user id: 0
m user id: 4206
owner id: 22022
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1602021_sequence/1

BBa_K1468002
type: ComponentDefinition
persistent identity: BBa K1468002
display id: BBa_K1468002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pJ23110 + gene encoding ZsGreen
creator: Pedro Luis Dorado Morales
created: 2014-10-02T11:00:00Z
modified: 2015-05-08T01:10:38Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1847_
m user id: 0
owner id: 11756
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: -
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence: https://synbiohub.org/public/igem/BBa_K1468002_sequence/1

BBa_K1680018
type: ComponentDefinition
persistent identity: BBa K1680018
display id: BBa_K1680018
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Cre-Dronpa fusion
creator: Nicolai von K??gelgen
created: 2015-09-16T11:00:00Z
modified: 2016-01-26T10:15:36Z
discontinued: false
dominant: false
group u list: _2098_
m user id: 4206
owner id: 26366
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Protein coding region for a fusion construct of Cre Recombinase and 145N Dronpa with 9 amino acid linker. Oligomerisation of Dronpa should stop Cre from accessing DNA in the nucleus thereby inhibiting it until light deactivation of dronpa.
mutable notes: Assembled using 3A assembly and RFC25.
mutable provenance: gBlocks designed by Team Tuebingen
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: K1680001
sequence annotation: K1680007
sequence annotation: K1680006
sequence: https://synbiohub.org/public/igem/BBa_K1680018_sequence/1

K1680001
type: SequenceAnnotation
persistent identity: annotation2477357
display id: annotation2477357
version: 1
top level: BBa_K1680018
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1680018/annotation2477357/range2477357/1

https://synbiohub.org/public/igem/BBa_K1680018/annotation2477357/range2477357/1
type: Range
persistent identity: range2477357
display id: range2477357
version: 1
direction: direction/forward
top level: BBa_K1680018
start: 1
end: 21
orientation: inline

K1680001
type: SequenceAnnotation
persistent identity: annotation2477358
display id: annotation2477358
version: 1
top level: BBa_K1680018
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1680018/annotation2477358/range2477358/1

https://synbiohub.org/public/igem/BBa_K1680018/annotation2477358/range2477358/1
type: Range
persistent identity: range2477358
display id: range2477358
version: 1
direction: direction/forward
top level: BBa_K1680018
start: 1051
end: 1077
orientation: inline

K1680007
type: SequenceAnnotation
persistent identity: annotation2477355
display id: annotation2477355
version: 1
top level: BBa_K1680018
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1680018/annotation2477355/range2477355/1

https://synbiohub.org/public/igem/BBa_K1680018/annotation2477355/range2477355/1
type: Range
persistent identity: range2477355
display id: range2477355
version: 1
direction: direction/forward
top level: BBa_K1680018
start: 22
end: 1050
orientation: inline

K1680006
type: SequenceAnnotation
persistent identity: annotation2477354
display id: annotation2477354
version: 1
top level: BBa_K1680018
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1680018/annotation2477354/range2477354/1

https://synbiohub.org/public/igem/BBa_K1680018/annotation2477354/range2477354/1
type: Range
persistent identity: range2477354
display id: range2477354
version: 1
direction: direction/forward
top level: BBa_K1680018
start: 1078
end: 1746
orientation: inline

BBa_K1486038
type: ComponentDefinition
persistent identity: BBa K1486038
display id: BBa_K1486038
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: sfGFPN
creator: EPFL iGem team 2014
created: 2014-09-26T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: To edit
mutable notes: To edit
mutable provenance: To edit
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence: https://synbiohub.org/public/igem/BBa_K1486038_sequence/1

BBa_K1486041
type: ComponentDefinition
persistent identity: BBa K1486041
display id: BBa_K1486041
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: sfGFPC + CpxR
creator: Ione Pla Puigsubir??
created: 2014-09-26T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: To edit
mutable notes: To edit
mutable provenance: To edit
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K1486037
component: BBa_I0500
component: BBa_K1486000
component: BBa_K1486039
component: BBa_B0034
sequence annotation: BBa_B0034
sequence annotation: BBa_I0500
sequence annotation: BBa_K1486039
sequence annotation: BBa_K1486000
sequence annotation: BBa_K1486037
sequence: https://synbiohub.org/public/igem/BBa_K1486041_sequence/1

BBa_K1486037
type: Component
persistent identity: component2387213
display id: component2387213
version: 1
top level: BBa_K1486041
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486037/1

BBa_I0500
type: Component
persistent identity: component2387209
display id: component2387209
version: 1
top level: BBa_K1486041
access: public
definition: https://synbiohub.org/public/igem/BBa_I0500/1

BBa_K1486000
type: Component
persistent identity: component2387217
display id: component2387217
version: 1
top level: BBa_K1486041
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486000/1

BBa_K1486039
type: Component
persistent identity: component2387212
display id: component2387212
version: 1
top level: BBa_K1486041
access: public
definition: BBa_K1486039

BBa_B0034
type: Component
persistent identity: component2387211
display id: component2387211
version: 1
top level: BBa_K1486041
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2387211
display id: annotation2387211
version: 1
top level: BBa_K1486041
location: https://synbiohub.org/public/igem/BBa_K1486041/annotation2387211/range2387211/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1486041/annotation2387211/range2387211/1
type: Range
persistent identity: range2387211
display id: range2387211
version: 1
direction: direction/reverse
top level: BBa_K1486041
start: 1211
end: 1222
orientation: inline

BBa_I0500
type: SequenceAnnotation
persistent identity: annotation2387209
display id: annotation2387209
version: 1
top level: BBa_K1486041
location: https://synbiohub.org/public/igem/BBa_K1486041/annotation2387209/range2387209/1
component: BBa_I0500

https://synbiohub.org/public/igem/BBa_K1486041/annotation2387209/range2387209/1
type: Range
persistent identity: range2387209
display id: range2387209
version: 1
direction: direction/reverse
top level: BBa_K1486041
start: 1
end: 1210
orientation: inline

BBa_K1486039
type: SequenceAnnotation
persistent identity: annotation2387212
display id: annotation2387212
version: 1
top level: BBa_K1486041
location: https://synbiohub.org/public/igem/BBa_K1486041/annotation2387212/range2387212/1
component: BBa_K1486039

https://synbiohub.org/public/igem/BBa_K1486041/annotation2387212/range2387212/1
type: Range
persistent identity: range2387212
display id: range2387212
version: 1
direction: direction/reverse
top level: BBa_K1486041
start: 1223
end: 1294
orientation: inline

BBa_K1486000
type: SequenceAnnotation
persistent identity: annotation2387217
display id: annotation2387217
version: 1
top level: BBa_K1486041
location: https://synbiohub.org/public/igem/BBa_K1486041/annotation2387217/range2387217/1
component: BBa_K1486000

https://synbiohub.org/public/igem/BBa_K1486041/annotation2387217/range2387217/1
type: Range
persistent identity: range2387217
display id: range2387217
version: 1
direction: direction/reverse
top level: BBa_K1486041
start: 1334
end: 2035
orientation: inline

BBa_K1486037
type: SequenceAnnotation
persistent identity: annotation2387213
display id: annotation2387213
version: 1
top level: BBa_K1486041
location: https://synbiohub.org/public/igem/BBa_K1486041/annotation2387213/range2387213/1
component: BBa_K1486037

https://synbiohub.org/public/igem/BBa_K1486041/annotation2387213/range2387213/1
type: Range
persistent identity: range2387213
display id: range2387213
version: 1
direction: direction/reverse
top level: BBa_K1486041
start: 1295
end: 1333
orientation: inline

BBa_K1909013
type: ComponentDefinition
persistent identity: BBa K1909013
display id: BBa_K1909013
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Omp Promoter / GFP
creator: Daniel Wedemeyer
created: 2016-10-13T11:00:00Z
modified: 2016-10-14T12:55:41Z
discontinued: false
dominant: false
group u list: _2375_
m user id: 29525
owner id: 29525
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Coming Soon
mutable notes: Coming Soon
mutable provenance: Composite Part
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_E0240
component: BBa_R0082
sequence annotation: BBa_R0082
sequence annotation: BBa_E0240
sequence: https://synbiohub.org/public/igem/BBa_K1909013_sequence/1

BBa_E0240
type: Component
persistent identity: component2508847
display id: component2508847
version: 1
top level: BBa_K1909013
access: public
definition: https://synbiohub.org/public/igem/BBa_E0240/1

BBa_R0082
type: Component
persistent identity: component2508836
display id: component2508836
version: 1
top level: BBa_K1909013
access: public
definition: https://synbiohub.org/public/igem/BBa_R0082/1

BBa_R0082
type: SequenceAnnotation
persistent identity: annotation2508836
display id: annotation2508836
version: 1
top level: BBa_K1909013
location: https://synbiohub.org/public/igem/BBa_K1909013/annotation2508836/range2508836/1
component: BBa_R0082

https://synbiohub.org/public/igem/BBa_K1909013/annotation2508836/range2508836/1
type: Range
persistent identity: range2508836
display id: range2508836
version: 1
direction: direction/reverse
top level: BBa_K1909013
start: 1
end: 108
orientation: inline

BBa_E0240
type: SequenceAnnotation
persistent identity: annotation2508847
display id: annotation2508847
version: 1
top level: BBa_K1909013
location: https://synbiohub.org/public/igem/BBa_K1909013/annotation2508847/range2508847/1
component: BBa_E0240

https://synbiohub.org/public/igem/BBa_K1909013/annotation2508847/range2508847/1
type: Range
persistent identity: range2508847
display id: range2508847
version: 1
direction: direction/reverse
top level: BBa_K1909013
start: 117
end: 992
orientation: inline

BBa_K1399021
type: ComponentDefinition
persistent identity: BBa K1399021
display id: BBa_K1399021
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Ptet-GFP(LAA)
creator: Anna Stikane
created: 2014-09-18T11:00:00Z
modified: 2015-05-08T01:10:16Z
discontinued: false
dominant: false
group u list: _1777_
m user id: 0
owner id: 22477
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Tetracycline/aTc inducible promoter with GFP reporter tagged with LAA-ssrA degradation tag followed by terminator. The tagged GFP is actively degraded within cell, thus provides better temporal resolution of green fluorescence and promoter activity.
mutable notes: Part was assembled using BrickClip assembly (BBF RFC104) using other biobrick parts as templates. BrickClip assembly is a special case of more general Paperclip assembly method.[1]
mutable provenance: other parts
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_R0040
component: BBa_B0015
component: BBa_B0034
component: BBa_K1399006
sequence annotation: BBa_K1399006
sequence annotation: BBa_R0040
sequence annotation: BBa_B0034
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_K1399021_sequence/1

BBa_R0040
type: Component
persistent identity: component2384259
display id: component2384259
version: 1
top level: BBa_K1399021
access: public
definition: https://synbiohub.org/public/igem/BBa_R0040/1

BBa_B0015
type: Component
persistent identity: component2384278
display id: component2384278
version: 1
top level: BBa_K1399021
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_B0034
type: Component
persistent identity: component2384265
display id: component2384265
version: 1
top level: BBa_K1399021
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K1399006
type: Component
persistent identity: component2384271
display id: component2384271
version: 1
top level: BBa_K1399021
access: public
definition: https://synbiohub.org/public/igem/BBa_K1399006/1

BBa_K1399006
type: SequenceAnnotation
persistent identity: annotation2384271
display id: annotation2384271
version: 1
top level: BBa_K1399021
location: https://synbiohub.org/public/igem/BBa_K1399021/annotation2384271/range2384271/1
component: BBa_K1399006

https://synbiohub.org/public/igem/BBa_K1399021/annotation2384271/range2384271/1
type: Range
persistent identity: range2384271
display id: range2384271
version: 1
direction: direction/reverse
top level: BBa_K1399021
start: 81
end: 833
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation2384259
display id: annotation2384259
version: 1
top level: BBa_K1399021
location: https://synbiohub.org/public/igem/BBa_K1399021/annotation2384259/range2384259/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_K1399021/annotation2384259/range2384259/1
type: Range
persistent identity: range2384259
display id: range2384259
version: 1
direction: direction/forward
top level: BBa_K1399021
start: 1
end: 54
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2384265
display id: annotation2384265
version: 1
top level: BBa_K1399021
location: https://synbiohub.org/public/igem/BBa_K1399021/annotation2384265/range2384265/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1399021/annotation2384265/range2384265/1
type: Range
persistent identity: range2384265
display id: range2384265
version: 1
direction: direction/reverse
top level: BBa_K1399021
start: 63
end: 74
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2384278
display id: annotation2384278
version: 1
top level: BBa_K1399021
location: https://synbiohub.org/public/igem/BBa_K1399021/annotation2384278/range2384278/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K1399021/annotation2384278/range2384278/1
type: Range
persistent identity: range2384278
display id: range2384278
version: 1
direction: direction/reverse
top level: BBa_K1399021
start: 842
end: 970
orientation: inline

BBa_K1680006
type: ComponentDefinition
persistent identity: BBa K1680006
display id: BBa_K1680006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Fluorescent protein dronpa
creator: Katharina Sporbeck
created: 2015-09-16T11:00:00Z
modified: 2016-01-26T10:15:05Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2098_
m user id: 4206
owner id: 26460
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Protein coding sequence for a mutant (K145N) of the fluorescent protein Dronpa, codon optimized for Yeast. The mutation leads to photoswitchable monomerisation/multimerisation behaviour (as described by Zhou et al. 2012).
mutable notes: Part is shipped in RFC25.
mutable provenance: gBlock designed by Team Tuebingen
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Dronpa
sequence: https://synbiohub.org/public/igem/BBa_K1680006_sequence/1

Dronpa
type: SequenceAnnotation
persistent identity: annotation2475196
display id: annotation2475196
version: 1
top level: BBa_K1680006
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1680006/annotation2475196/range2475196/1

https://synbiohub.org/public/igem/BBa_K1680006/annotation2475196/range2475196/1
type: Range
persistent identity: range2475196
display id: range2475196
version: 1
direction: direction/forward
top level: BBa_K1680006
start: 1
end: 669
orientation: inline

GFP-Si4Tag
type: ComponentDefinition
persistent identity: BBa K1602024
display id: BBa_K1602024
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: HisTag-GFP_Si4-Tag fusion protein
creator: Jasmin Weber, Michelle Zoeller, Marcus Geissler, Stefan Bohn, Simon Linder, Sebastian Barthel
created: 2015-09-15T11:00:00Z
modified: 2016-01-25T01:11:28Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2019_
m user id: 4206
owner id: 22022
owning group id: 9
status: status/Planning
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1602024_sequence/1

BBa_K1497020
type: ComponentDefinition
persistent identity: BBa K1497020
display id: BBa_K1497020
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Naringenin sensor (FdeR) with GFP as reporter
creator: Sascha Hein, Niklas Hummel, Sebastian Barthel, Thomas Dohmen
created: 2014-10-06T11:00:00Z
modified: 2015-05-08T01:10:45Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1877_
m user id: 0
owner id: 12466
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: We modified the naringenin biosensor from Siedler et al. by elimination of one EcoRI restriction site and by changing the native ribosomal binding site into the strong RBS Bba_B0034. Based on this reporter protein and 3 different fluorescence proteins, we designed 3 new biosensors for in vivo detection and determination of naringenin. The natural source of FdeR is Herbaspirillum seropedicae. You can use our reporter for measuring naringenin concentrations in your samples. Depending on which fluorophor you want to detect, you can use one of three biosensors: GFP fluorescence K1497020 mKate (DsRed variant) fluorescence K1497021 CFP fluorescence K1497022 You can create your own naringenin sensor or your own naringenin dependent gene expression device as well. For these reasons use FdeR Biobrick K1497019 and clone your parts of interest (without RBS!) behind the device.
mutable notes: d
mutable provenance: d
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_K1497019
component: BBa_E0040
sequence annotation: BBa_K1497019
sequence annotation: BBa_E0040
sequence: https://synbiohub.org/public/igem/BBa_K1497020_sequence/1

BBa_K1497019
type: Component
persistent identity: component2398947
display id: component2398947
version: 1
top level: BBa_K1497020
access: public
definition: https://synbiohub.org/public/igem/BBa_K1497019/1

BBa_E0040
type: Component
persistent identity: component2398949
display id: component2398949
version: 1
top level: BBa_K1497020
access: public
definition: GFP

BBa_K1497019
type: SequenceAnnotation
persistent identity: annotation2398947
display id: annotation2398947
version: 1
top level: BBa_K1497020
location: https://synbiohub.org/public/igem/BBa_K1497020/annotation2398947/range2398947/1
component: BBa_K1497019

https://synbiohub.org/public/igem/BBa_K1497020/annotation2398947/range2398947/1
type: Range
persistent identity: range2398947
display id: range2398947
version: 1
direction: direction/reverse
top level: BBa_K1497020
start: 1
end: 1214
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation2398949
display id: annotation2398949
version: 1
top level: BBa_K1497020
location: https://synbiohub.org/public/igem/BBa_K1497020/annotation2398949/range2398949/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_K1497020/annotation2398949/range2398949/1
type: Range
persistent identity: range2398949
display id: range2398949
version: 1
direction: direction/reverse
top level: BBa_K1497020
start: 1221
end: 1940
orientation: inline

BBa_K1468000
type: ComponentDefinition
persistent identity: BBa K1468000
display id: BBa_K1468000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pJ23104 + gene encoding ZsGreen
creator: Pedro Luis Dorado Morales
created: 2014-10-02T11:00:00Z
modified: 2015-05-08T01:10:38Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1847_
m user id: 0
owner id: 11756
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: -
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: ZsGreeen
sequence annotation: ZsGreen protein
sequence annotation: START
sequence annotation: STOP
sequence annotation: J23104p
sequence annotation: BBa_E0040
sequence annotation: BBa_J23104
sequence annotation: BBa_K1468000
sequence: https://synbiohub.org/public/igem/BBa_K1468000_sequence/1

ZsGreeen
type: SequenceAnnotation
persistent identity: annotation2418062
display id: annotation2418062
version: 1
top level: BBa_K1468000
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1468000/annotation2418062/range2418062/1

https://synbiohub.org/public/igem/BBa_K1468000/annotation2418062/range2418062/1
type: Range
persistent identity: range2418062
display id: range2418062
version: 1
direction: direction/forward
top level: BBa_K1468000
start: 34
end: 725
orientation: inline

ZsGreen protein
type: SequenceAnnotation
persistent identity: annotation2418059
display id: annotation2418059
version: 1
top level: BBa_K1468000
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1468000/annotation2418059/range2418059/1

https://synbiohub.org/public/igem/BBa_K1468000/annotation2418059/range2418059/1
type: Range
persistent identity: range2418059
display id: range2418059
version: 1
direction: direction/forward
top level: BBa_K1468000
start: 34
end: 725
orientation: inline

START
type: SequenceAnnotation
persistent identity: annotation2418061
display id: annotation2418061
version: 1
top level: BBa_K1468000
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1468000/annotation2418061/range2418061/1

https://synbiohub.org/public/igem/BBa_K1468000/annotation2418061/range2418061/1
type: Range
persistent identity: range2418061
display id: range2418061
version: 1
direction: direction/forward
top level: BBa_K1468000
start: 34
end: 36
orientation: inline

STOP
type: SequenceAnnotation
persistent identity: annotation2418064
display id: annotation2418064
version: 1
top level: BBa_K1468000
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1468000/annotation2418064/range2418064/1

https://synbiohub.org/public/igem/BBa_K1468000/annotation2418064/range2418064/1
type: Range
persistent identity: range2418064
display id: range2418064
version: 1
direction: direction/forward
top level: BBa_K1468000
start: 725
end: 725
orientation: inline

J23104p
type: SequenceAnnotation
persistent identity: annotation2418030
display id: annotation2418030
version: 1
top level: BBa_K1468000
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1468000/annotation2418030/range2418030/1

https://synbiohub.org/public/igem/BBa_K1468000/annotation2418030/range2418030/1
type: Range
persistent identity: range2418030
display id: range2418030
version: 1
direction: direction/forward
top level: BBa_K1468000
start: 1
end: 33
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation2418060
display id: annotation2418060
version: 1
top level: BBa_K1468000
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1468000/annotation2418060/range2418060/1

https://synbiohub.org/public/igem/BBa_K1468000/annotation2418060/range2418060/1
type: Range
persistent identity: range2418060
display id: range2418060
version: 1
direction: direction/forward
top level: BBa_K1468000
start: 34
end: 725
orientation: inline

BBa_J23104
type: SequenceAnnotation
persistent identity: annotation2418058
display id: annotation2418058
version: 1
top level: BBa_K1468000
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1468000/annotation2418058/range2418058/1

https://synbiohub.org/public/igem/BBa_K1468000/annotation2418058/range2418058/1
type: Range
persistent identity: range2418058
display id: range2418058
version: 1
direction: direction/forward
top level: BBa_K1468000
start: 1
end: 33
orientation: inline

BBa_K1468000
type: SequenceAnnotation
persistent identity: annotation2418810
display id: annotation2418810
version: 1
top level: BBa_K1468000
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1468000/annotation2418810/range2418810/1

https://synbiohub.org/public/igem/BBa_K1468000/annotation2418810/range2418810/1
type: Range
persistent identity: range2418810
display id: range2418810
version: 1
direction: direction/forward
top level: BBa_K1468000
start: 1
end: 725
orientation: inline

BBa_K1486002
type: ComponentDefinition
persistent identity: BBa K1486002
display id: BBa_K1486002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Arabinose promoter + sfGFP CpxR (Nterm)
creator: iGem EPFL 2014
created: 2014-08-11T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part is composed of an arabinose-inducible promoter for superfolded GFP attached by a linker to CpxR (on the N terminus of CpxR).
mutable notes: We had to test both attachments, N and C terminus of the CpxR.
mutable provenance: The arabinose promoter and the sfGFP were obtained from the registry, while the CpxR was obtained from the E coli genome.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_I0500
component: BBa_I746916
component: BBa_K1486003
component: BBa_K1486000
component: BBa_B0034
sequence annotation: BBa_I0500
sequence annotation: BBa_K1486000
sequence annotation: BBa_K1486003
sequence annotation: BBa_I746916
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K1486002_sequence/1

BBa_I0500
type: Component
persistent identity: component2382057
display id: component2382057
version: 1
top level: BBa_K1486002
access: public
definition: https://synbiohub.org/public/igem/BBa_I0500/1

BBa_I746916
type: Component
persistent identity: component2382063
display id: component2382063
version: 1
top level: BBa_K1486002
access: public
definition: https://synbiohub.org/public/igem/BBa_I746916/1

BBa_K1486003
type: Component
persistent identity: component2382064
display id: component2382064
version: 1
top level: BBa_K1486002
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486003/1

BBa_K1486000
type: Component
persistent identity: component2382068
display id: component2382068
version: 1
top level: BBa_K1486002
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486000/1

BBa_B0034
type: Component
persistent identity: component2382059
display id: component2382059
version: 1
top level: BBa_K1486002
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_I0500
type: SequenceAnnotation
persistent identity: annotation2382057
display id: annotation2382057
version: 1
top level: BBa_K1486002
location: https://synbiohub.org/public/igem/BBa_K1486002/annotation2382057/range2382057/1
component: BBa_I0500

https://synbiohub.org/public/igem/BBa_K1486002/annotation2382057/range2382057/1
type: Range
persistent identity: range2382057
display id: range2382057
version: 1
direction: direction/reverse
top level: BBa_K1486002
start: 1
end: 1210
orientation: inline

BBa_K1486000
type: SequenceAnnotation
persistent identity: annotation2382068
display id: annotation2382068
version: 1
top level: BBa_K1486002
location: https://synbiohub.org/public/igem/BBa_K1486002/annotation2382068/range2382068/1
component: BBa_K1486000

https://synbiohub.org/public/igem/BBa_K1486002/annotation2382068/range2382068/1
type: Range
persistent identity: range2382068
display id: range2382068
version: 1
direction: direction/reverse
top level: BBa_K1486002
start: 1967
end: 2668
orientation: inline

BBa_K1486003
type: SequenceAnnotation
persistent identity: annotation2382064
display id: annotation2382064
version: 1
top level: BBa_K1486002
location: https://synbiohub.org/public/igem/BBa_K1486002/annotation2382064/range2382064/1
component: BBa_K1486003

https://synbiohub.org/public/igem/BBa_K1486002/annotation2382064/range2382064/1
type: Range
persistent identity: range2382064
display id: range2382064
version: 1
direction: direction/reverse
top level: BBa_K1486002
start: 1943
end: 1966
orientation: inline

BBa_I746916
type: SequenceAnnotation
persistent identity: annotation2382063
display id: annotation2382063
version: 1
top level: BBa_K1486002
location: https://synbiohub.org/public/igem/BBa_K1486002/annotation2382063/range2382063/1
component: BBa_I746916

https://synbiohub.org/public/igem/BBa_K1486002/annotation2382063/range2382063/1
type: Range
persistent identity: range2382063
display id: range2382063
version: 1
direction: direction/reverse
top level: BBa_K1486002
start: 1223
end: 1942
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2382059
display id: annotation2382059
version: 1
top level: BBa_K1486002
location: https://synbiohub.org/public/igem/BBa_K1486002/annotation2382059/range2382059/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1486002/annotation2382059/range2382059/1
type: Range
persistent identity: range2382059
display id: range2382059
version: 1
direction: direction/reverse
top level: BBa_K1486002
start: 1211
end: 1222
orientation: inline

BBa_K1789004
type: ComponentDefinition
persistent identity: BBa K1789004
display id: BBa_K1789004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: GFP2
creator: Xinyuan Qiu
created: 2015-09-13T11:00:00Z
modified: 2016-01-25T01:04:48Z
discontinued: false
dominant: false
group u list: _2214_
m user id: 4206
owner id: 21134
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This part is the Carboxyl Half of GFP
mutable notes: we REMOVED the extra base from part BBa_I715020(easier to fuse with other proteins) when designing PCR primers to make the protein fusion easier.
mutable provenance: This part is gained from part BBa_I715020.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: GFP2
sequence: https://synbiohub.org/public/igem/BBa_K1789004_sequence/1

GFP2
type: SequenceAnnotation
persistent identity: annotation2454067
display id: annotation2454067
version: 1
top level: BBa_K1789004
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1789004/annotation2454067/range2454067/1

https://synbiohub.org/public/igem/BBa_K1789004/annotation2454067/range2454067/1
type: Range
persistent identity: range2454067
display id: range2454067
version: 1
direction: direction/forward
top level: BBa_K1789004
start: 1
end: 249
orientation: inline

BBa_K1399016
type: ComponentDefinition
persistent identity: BBa K1399016
display id: BBa_K1399016
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Plac-GFP(LAA)
creator: Anna Stikane
created: 2014-09-18T11:00:00Z
modified: 2015-05-08T01:10:16Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1777_
m user id: 0
owner id: 22477
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: Lactose/IPTG inducible promoter with GFP reporter tagged with AAV-ssrA degradation tag followed by terminator. The tagged GFP is actively degraded within cell, thus provides better temporal resolution of green fluorescence and promoter activity.
mutable notes: Part was assembled using BrickClip assembly (BBF RFC104) using other biobrick parts as templates. BrickClip assembly is a special case of more general Paperclip assembly method.[1]
mutable provenance: other biobricks
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_R0010
component: BBa_B0015
component: BBa_B0034
component: BBa_K1399006
sequence annotation: BBa_R0010
sequence annotation: BBa_B0034
sequence annotation: BBa_K1399006
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_K1399016_sequence/1

BBa_R0010
type: Component
persistent identity: component2384145
display id: component2384145
version: 1
top level: BBa_K1399016
access: public
definition: https://synbiohub.org/public/igem/BBa_R0010/1

BBa_B0015
type: Component
persistent identity: component2384166
display id: component2384166
version: 1
top level: BBa_K1399016
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_B0034
type: Component
persistent identity: component2384153
display id: component2384153
version: 1
top level: BBa_K1399016
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K1399006
type: Component
persistent identity: component2384159
display id: component2384159
version: 1
top level: BBa_K1399016
access: public
definition: https://synbiohub.org/public/igem/BBa_K1399006/1

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation2384145
display id: annotation2384145
version: 1
top level: BBa_K1399016
location: https://synbiohub.org/public/igem/BBa_K1399016/annotation2384145/range2384145/1
component: BBa_R0010

https://synbiohub.org/public/igem/BBa_K1399016/annotation2384145/range2384145/1
type: Range
persistent identity: range2384145
display id: range2384145
version: 1
direction: direction/forward
top level: BBa_K1399016
start: 1
end: 200
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2384153
display id: annotation2384153
version: 1
top level: BBa_K1399016
location: https://synbiohub.org/public/igem/BBa_K1399016/annotation2384153/range2384153/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1399016/annotation2384153/range2384153/1
type: Range
persistent identity: range2384153
display id: range2384153
version: 1
direction: direction/reverse
top level: BBa_K1399016
start: 209
end: 220
orientation: inline

BBa_K1399006
type: SequenceAnnotation
persistent identity: annotation2384159
display id: annotation2384159
version: 1
top level: BBa_K1399016
location: https://synbiohub.org/public/igem/BBa_K1399016/annotation2384159/range2384159/1
component: BBa_K1399006

https://synbiohub.org/public/igem/BBa_K1399016/annotation2384159/range2384159/1
type: Range
persistent identity: range2384159
display id: range2384159
version: 1
direction: direction/reverse
top level: BBa_K1399016
start: 227
end: 979
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2384166
display id: annotation2384166
version: 1
top level: BBa_K1399016
location: https://synbiohub.org/public/igem/BBa_K1399016/annotation2384166/range2384166/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K1399016/annotation2384166/range2384166/1
type: Range
persistent identity: range2384166
display id: range2384166
version: 1
direction: direction/reverse
top level: BBa_K1399016
start: 988
end: 1116
orientation: inline

BBa_K1824100
type: ComponentDefinition
persistent identity: BBa K1824100
display id: BBa_K1824100
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: eGFP-terminater
creator: Xinhao Wang
created: 2015-09-12T11:00:00Z
modified: 2015-09-13T02:49:04Z
discontinued: false
dominant: false
group u list: _2250_
m user id: 26000
owner id: 26000
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Composed of eGFP-rrnB terminator-T7 terminater, canbe used in further assembly.
mutable notes: Mind the 5'UTR if in a complex construct.
mutable provenance: synthetic construct
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1824889
component: BBa_B0015
component: BBa_K1094400
sequence annotation: BBa_K1094400
sequence annotation: BBa_K1824889
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_K1824100_sequence/1

BBa_K1824889
type: Component
persistent identity: component2453149
display id: component2453149
version: 1
top level: BBa_K1824100
access: public
definition: https://synbiohub.org/public/igem/BBa_K1824889/1

BBa_B0015
type: Component
persistent identity: component2453156
display id: component2453156
version: 1
top level: BBa_K1824100
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_K1094400
type: Component
persistent identity: component2453148
display id: component2453148
version: 1
top level: BBa_K1824100
access: public
definition: https://synbiohub.org/public/igem/BBa_K1094400/1

BBa_K1094400
type: SequenceAnnotation
persistent identity: annotation2453148
display id: annotation2453148
version: 1
top level: BBa_K1824100
location: https://synbiohub.org/public/igem/BBa_K1824100/annotation2453148/range2453148/1
component: BBa_K1094400

https://synbiohub.org/public/igem/BBa_K1824100/annotation2453148/range2453148/1
type: Range
persistent identity: range2453148
display id: range2453148
version: 1
direction: direction/reverse
top level: BBa_K1824100
start: 1
end: 723
orientation: inline

BBa_K1824889
type: SequenceAnnotation
persistent identity: annotation2453149
display id: annotation2453149
version: 1
top level: BBa_K1824100
location: https://synbiohub.org/public/igem/BBa_K1824100/annotation2453149/range2453149/1
component: BBa_K1824889

https://synbiohub.org/public/igem/BBa_K1824100/annotation2453149/range2453149/1
type: Range
persistent identity: range2453149
display id: range2453149
version: 1
direction: direction/reverse
top level: BBa_K1824100
start: 724
end: 731
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2453156
display id: annotation2453156
version: 1
top level: BBa_K1824100
location: https://synbiohub.org/public/igem/BBa_K1824100/annotation2453156/range2453156/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K1824100/annotation2453156/range2453156/1
type: Range
persistent identity: range2453156
display id: range2453156
version: 1
direction: direction/reverse
top level: BBa_K1824100
start: 732
end: 860
orientation: inline

BBa_K1321348
type: ComponentDefinition
persistent identity: BBa K1321348
display id: BBa_K1321348
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: sfGFP fused to dCBD with linker in RFC 25
creator: Chris N Micklem
created: 2014-10-07T11:00:00Z
modified: 2015-06-17T12:30:41Z
discontinued: false
dominant: false
group u list: _1696_
m user id: 4206
owner id: 20830
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: asdf
mutable notes: asdf
mutable provenance: asdf
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_K1321340
component: BBa_K1321337
component: BBa_B0105
sequence annotation: BBa_K1321340
sequence annotation: BBa_K1321337
sequence annotation: BBa_B0105
sequence: https://synbiohub.org/public/igem/BBa_K1321348_sequence/1

BBa_K1321340
type: Component
persistent identity: component2415140
display id: component2415140
version: 1
top level: BBa_K1321348
access: public
definition: https://synbiohub.org/public/igem/BBa_K1321340/1

BBa_K1321337
type: Component
persistent identity: component2415136
display id: component2415136
version: 1
top level: BBa_K1321348
access: public
definition: BBa_K1321337

BBa_B0105
type: Component
persistent identity: component2415138
display id: component2415138
version: 1
top level: BBa_K1321348
access: public
definition: https://synbiohub.org/public/igem/BBa_B0105/1

BBa_K1321340
type: SequenceAnnotation
persistent identity: annotation2415140
display id: annotation2415140
version: 1
top level: BBa_K1321348
location: https://synbiohub.org/public/igem/BBa_K1321348/annotation2415140/range2415140/1
component: BBa_K1321340

https://synbiohub.org/public/igem/BBa_K1321348/annotation2415140/range2415140/1
type: Range
persistent identity: range2415140
display id: range2415140
version: 1
direction: direction/reverse
top level: BBa_K1321348
start: 718
end: 1083
orientation: inline

BBa_K1321337
type: SequenceAnnotation
persistent identity: annotation2415136
display id: annotation2415136
version: 1
top level: BBa_K1321348
location: https://synbiohub.org/public/igem/BBa_K1321348/annotation2415136/range2415136/1
component: BBa_K1321337

https://synbiohub.org/public/igem/BBa_K1321348/annotation2415136/range2415136/1
type: Range
persistent identity: range2415136
display id: range2415136
version: 1
direction: direction/reverse
top level: BBa_K1321348
start: 1
end: 711
orientation: inline

BBa_B0105
type: SequenceAnnotation
persistent identity: annotation2415138
display id: annotation2415138
version: 1
top level: BBa_K1321348
location: https://synbiohub.org/public/igem/BBa_K1321348/annotation2415138/range2415138/1
component: BBa_B0105

https://synbiohub.org/public/igem/BBa_K1321348/annotation2415138/range2415138/1
type: Range
persistent identity: range2415138
display id: range2415138
version: 1
direction: direction/reverse
top level: BBa_K1321348
start: 712
end: 717
orientation: inline

BBa_K1742003
type: ComponentDefinition
persistent identity: BBa K1742003
display id: BBa_K1742003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Dronpa 145K
creator: Iv??n Casas Rodrigo
created: 2015-09-03T11:00:00Z
modified: 2016-01-25T11:27:33Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2164_
m user id: 4206
owner id: 26598
owning group id: 9
status: status/Planning
bookmark: false
mutable description: From Pectinidae sp. It is a CDS of green fluorescent protein Dronpa.
mutable notes: It is codon optimized for plants.
mutable provenance: Pectinidae sp. sequence found in the following article Lummer, M., Humpert, F., Steuwe, C., Caesar, K., Sch??ttpelz, M., Sauer, M. and Staiger, D. (2011), Reversible Photoswitchable DRONPA-s Monitors Nucleocytoplasmic Transport of an RNA-Binding Protein in Transgenic Plants. Traffic, 12: 693???702. doi: 10.1111/j.1600-0854.2011.01180.x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1742003_sequence/1

BBa_K1321337
type: ComponentDefinition
persistent identity: BBa K1321337
display id: BBa_K1321337
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: sfGFP in Freiburg format (RFC 25)
creator: Chris N Micklem
created: 2014-10-07T11:00:00Z
modified: 2016-01-26T02:10:16Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1696_
m user id: 4206
owner id: 20830
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: sfGFP in Freiburg format (RFC 25) to allow for easy use in fusion proteins.
mutable notes: OEPCR
mutable provenance: Made from existing part.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: sfGFP
sequence: https://synbiohub.org/public/igem/BBa_K1321337_sequence/1

sfGFP
type: SequenceAnnotation
persistent identity: annotation2417826
display id: annotation2417826
version: 1
top level: BBa_K1321337
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1321337/annotation2417826/range2417826/1

https://synbiohub.org/public/igem/BBa_K1321337/annotation2417826/range2417826/1
type: Range
persistent identity: range2417826
display id: range2417826
version: 1
direction: direction/forward
top level: BBa_K1321337
start: 1
end: 711
orientation: inline

GFP
type: ComponentDefinition
persistent identity: BBa E0040
display id: BBa_E0040
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212
creator: jcbraff
created: 2004-09-29T11:00:00Z
modified: 2016-01-26T02:09:38Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _11_1_
m user id: 4206
owner id: 61
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: GFP (mut3b) [note that this part does not have a barcode]
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: GFP protein
sequence: https://synbiohub.org/public/igem/BBa_E0040_sequence/1

GFP protein
type: SequenceAnnotation
persistent identity: annotation1934520
display id: annotation1934520
version: 1
top level: GFP
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_E0040/annotation1934520/range1934520/1

https://synbiohub.org/public/igem/BBa_E0040/annotation1934520/range1934520/1
type: Range
persistent identity: range1934520
display id: range1934520
version: 1
direction: direction/forward
top level: GFP
start: 1
end: 720
orientation: inline

BBa_J52021
type: ComponentDefinition
persistent identity: BBa J52021
display id: BBa_J52021
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: dnTraf6-linker-GFP
creator: Matej Skocaj
created: 2006-10-13T11:00:00Z
modified: 2016-01-26T09:38:59Z
discontinued: false
dominant: false
experience: experience/None
group u list: _80_
m user id: 0
m user id: 4206
owner id: 857
owning group id: 80
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Part is composed of TIR domain of Traf6 and GFP linked with 6 aminoacids long linker. GFP serves as reporter. For usage in eukaryotes.
mutable notes: None.
mutable provenance: dnTraf6- pDeNy GFP-
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: GFP
sequence annotation: dnTraf6-linker-GFP
sequence annotation: linker
sequence annotation: dnTraf6
sequence: https://synbiohub.org/public/igem/BBa_J52021_sequence/1

GFP
type: SequenceAnnotation
persistent identity: annotation1903261
display id: annotation1903261
version: 1
top level: BBa_J52021
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J52021/annotation1903261/range1903261/1

https://synbiohub.org/public/igem/BBa_J52021/annotation1903261/range1903261/1
type: Range
persistent identity: range1903261
display id: range1903261
version: 1
direction: direction/forward
top level: BBa_J52021
start: 733
end: 1446
orientation: inline

dnTraf6-linker-GFP
type: SequenceAnnotation
persistent identity: annotation1903262
display id: annotation1903262
version: 1
top level: BBa_J52021
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_J52021/annotation1903262/range1903262/1

https://synbiohub.org/public/igem/BBa_J52021/annotation1903262/range1903262/1
type: Range
persistent identity: range1903262
display id: range1903262
version: 1
direction: direction/forward
top level: BBa_J52021
start: 1
end: 1446
orientation: inline

linker
type: SequenceAnnotation
persistent identity: annotation1903260
display id: annotation1903260
version: 1
top level: BBa_J52021
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_J52021/annotation1903260/range1903260/1

https://synbiohub.org/public/igem/BBa_J52021/annotation1903260/range1903260/1
type: Range
persistent identity: range1903260
display id: range1903260
version: 1
direction: direction/forward
top level: BBa_J52021
start: 715
end: 732
orientation: inline

dnTraf6
type: SequenceAnnotation
persistent identity: annotation1903259
display id: annotation1903259
version: 1
top level: BBa_J52021
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J52021/annotation1903259/range1903259/1

https://synbiohub.org/public/igem/BBa_J52021/annotation1903259/range1903259/1
type: Range
persistent identity: range1903259
display id: range1903259
version: 1
direction: direction/forward
top level: BBa_J52021
start: 1
end: 714
orientation: inline

BBa_K1467204
type: ComponentDefinition
persistent identity: BBa K1467204
display id: BBa_K1467204
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: GoldenGate compatible Green Flourescent Protein (GFP)
creator: Cara Deal
created: 2014-09-23T11:00:00Z
modified: 2015-06-01T07:05:42Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1846_
m user id: 4206
owner id: 21550
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: A variation of the part BBa_E0040 that has been made compatible for Golden Gate by removal of internal BsaI and BpiI recognition sequences. Fluoresces under UV light when the gene is expressed.
mutable notes: Domestication for GoldenGate by removal of internal BsaI and BpiI recognition sequences.
mutable provenance: Aequorea victoria
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence: https://synbiohub.org/public/igem/BBa_K1467204_sequence/1

BBa_K1980008
type: ComponentDefinition
persistent identity: BBa K1980008
display id: BBa_K1980008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pCopA CueR sfGFP/ feedback pCopA sfGFP
creator: Sam Garforth
created: 2016-10-10T11:00:00Z
modified: 2016-10-24T07:34:54Z
discontinued: false
dominant: false
experience: experience/Issues
group u list: _2447_
m user id: 29607
owner id: 29607
owning group id: 9
status: status/Planning
bookmark: false
mutable description: pCopA CueR sfGFP/ feedback pCopA sfGFP
mutable notes: codon op
mutable provenance: E. coli
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: RBS
sequence annotation: Stop
sequence annotation: pCopA
sequence annotation: sfGFP
sequence annotation: CueR
sequence annotation: Hexahistidine
sequence: https://synbiohub.org/public/igem/BBa_K1980008_sequence/1

RBS
type: SequenceAnnotation
persistent identity: annotation2528250
display id: annotation2528250
version: 1
top level: BBa_K1980008
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1980008/annotation2528250/range2528250/1

https://synbiohub.org/public/igem/BBa_K1980008/annotation2528250/range2528250/1
type: Range
persistent identity: range2528250
display id: range2528250
version: 1
direction: direction/forward
top level: BBa_K1980008
start: 58
end: 69
orientation: inline

Stop
type: SequenceAnnotation
persistent identity: annotation2528256
display id: annotation2528256
version: 1
top level: BBa_K1980008
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1980008/annotation2528256/range2528256/1

https://synbiohub.org/public/igem/BBa_K1980008/annotation2528256/range2528256/1
type: Range
persistent identity: range2528256
display id: range2528256
version: 1
direction: direction/forward
top level: BBa_K1980008
start: 481
end: 486
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2528253
display id: annotation2528253
version: 1
top level: BBa_K1980008
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1980008/annotation2528253/range2528253/1

https://synbiohub.org/public/igem/BBa_K1980008/annotation2528253/range2528253/1
type: Range
persistent identity: range2528253
display id: range2528253
version: 1
direction: direction/forward
top level: BBa_K1980008
start: 499
end: 504
orientation: inline

Stop
type: SequenceAnnotation
persistent identity: annotation2528255
display id: annotation2528255
version: 1
top level: BBa_K1980008
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1980008/annotation2528255/range2528255/1

https://synbiohub.org/public/igem/BBa_K1980008/annotation2528255/range2528255/1
type: Range
persistent identity: range2528255
display id: range2528255
version: 1
direction: direction/forward
top level: BBa_K1980008
start: 1247
end: 1252
orientation: inline

pCopA
type: SequenceAnnotation
persistent identity: annotation2528249
display id: annotation2528249
version: 1
top level: BBa_K1980008
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1980008/annotation2528249/range2528249/1

https://synbiohub.org/public/igem/BBa_K1980008/annotation2528249/range2528249/1
type: Range
persistent identity: range2528249
display id: range2528249
version: 1
direction: direction/forward
top level: BBa_K1980008
start: 1
end: 51
orientation: inline

sfGFP
type: SequenceAnnotation
persistent identity: annotation2528252
display id: annotation2528252
version: 1
top level: BBa_K1980008
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1980008/annotation2528252/range2528252/1

https://synbiohub.org/public/igem/BBa_K1980008/annotation2528252/range2528252/1
type: Range
persistent identity: range2528252
display id: range2528252
version: 1
direction: direction/forward
top level: BBa_K1980008
start: 515
end: 1228
orientation: inline

CueR
type: SequenceAnnotation
persistent identity: annotation2528251
display id: annotation2528251
version: 1
top level: BBa_K1980008
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1980008/annotation2528251/range2528251/1

https://synbiohub.org/public/igem/BBa_K1980008/annotation2528251/range2528251/1
type: Range
persistent identity: range2528251
display id: range2528251
version: 1
direction: direction/forward
top level: BBa_K1980008
start: 76
end: 480
orientation: inline

Hexahistidine
type: SequenceAnnotation
persistent identity: annotation2528254
display id: annotation2528254
version: 1
top level: BBa_K1980008
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1980008/annotation2528254/range2528254/1

https://synbiohub.org/public/igem/BBa_K1980008/annotation2528254/range2528254/1
type: Range
persistent identity: range2528254
display id: range2528254
version: 1
direction: direction/forward
top level: BBa_K1980008
start: 1229
end: 1246
orientation: inline

BBa_K1680017
type: ComponentDefinition
persistent identity: BBa K1680017
display id: BBa_K1680017
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Cre-Dronpa Fusion
creator: Nicolai von K??gelgen
created: 2015-09-16T11:00:00Z
modified: 2016-01-26T10:15:17Z
discontinued: false
dominant: false
group u list: _2098_
m user id: 4206
owner id: 26366
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Protein coding region for a fusion construct of Cre Recombinase and 145N Dronpa with intermediate 6 amino acid linker. Oligomerisation of Dronpa should stop Cre from accessing DNA in the nucleus thereby inhibiting it until light deactivation of dronpa.
mutable notes: Assembled using 3A assembly.
mutable provenance: gBlocks designed by Team Tuebingen.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: BBa_K1680007
sequence annotation: BBa_K1680000
sequence annotation: BBa_K1680006
sequence: https://synbiohub.org/public/igem/BBa_K1680017_sequence/1

BBa_K1680007
type: SequenceAnnotation
persistent identity: annotation2465977
display id: annotation2465977
version: 1
top level: BBa_K1680017
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1680017/annotation2465977/range2465977/1

https://synbiohub.org/public/igem/BBa_K1680017/annotation2465977/range2465977/1
type: Range
persistent identity: range2465977
display id: range2465977
version: 1
direction: direction/reverse
top level: BBa_K1680017
start: 13
end: 1041
orientation: inline

BBa_K1680000
type: SequenceAnnotation
persistent identity: annotation2465978
display id: annotation2465978
version: 1
top level: BBa_K1680017
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1680017/annotation2465978/range2465978/1

https://synbiohub.org/public/igem/BBa_K1680017/annotation2465978/range2465978/1
type: Range
persistent identity: range2465978
display id: range2465978
version: 1
direction: direction/reverse
top level: BBa_K1680017
start: 1050
end: 1055
orientation: inline

BBa_K1680006
type: SequenceAnnotation
persistent identity: annotation2465979
display id: annotation2465979
version: 1
top level: BBa_K1680017
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1680017/annotation2465979/range2465979/1

https://synbiohub.org/public/igem/BBa_K1680017/annotation2465979/range2465979/1
type: Range
persistent identity: range2465979
display id: range2465979
version: 1
direction: direction/reverse
top level: BBa_K1680017
start: 1064
end: 1732
orientation: inline

BBa_K1680000
type: SequenceAnnotation
persistent identity: annotation2465976
display id: annotation2465976
version: 1
top level: BBa_K1680017
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1680017/annotation2465976/range2465976/1

https://synbiohub.org/public/igem/BBa_K1680017/annotation2465976/range2465976/1
type: Range
persistent identity: range2465976
display id: range2465976
version: 1
direction: direction/reverse
top level: BBa_K1680017
start: 1
end: 6
orientation: inline

BBa_K1486023
type: ComponentDefinition
persistent identity: BBa K1486023
display id: BBa_K1486023
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Yeast optimized superfolder GFP
creator: EPFL iGem team 2014
created: 2014-09-25T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: To edit
mutable notes: To edit
mutable provenance: To edit
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: Yo sfGFP
sequence: https://synbiohub.org/public/igem/BBa_K1486023_sequence/1

Yo sfGFP
type: SequenceAnnotation
persistent identity: annotation2418775
display id: annotation2418775
version: 1
top level: BBa_K1486023
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1486023/annotation2418775/range2418775/1

https://synbiohub.org/public/igem/BBa_K1486023/annotation2418775/range2418775/1
type: Range
persistent identity: range2418775
display id: range2418775
version: 1
direction: direction/forward
top level: BBa_K1486023
start: 1
end: 717
orientation: inline

BBa_K1321342
type: ComponentDefinition
persistent identity: BBa K1321342
display id: BBa_K1321342
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: sfGFP fused to CBDcex in RFC 25
creator: Chris N Micklem
created: 2014-10-07T11:00:00Z
modified: 2015-06-17T12:26:42Z
discontinued: false
dominant: false
group u list: _1696_
m user id: 4206
owner id: 20830
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: sadf
mutable notes: asdf
mutable provenance: asdf
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_K1321003
component: BBa_K1321337
component: BBa_B0105
sequence annotation: BBa_K1321003
sequence annotation: BBa_B0105
sequence annotation: BBa_K1321337
sequence: https://synbiohub.org/public/igem/BBa_K1321342_sequence/1

BBa_K1321003
type: Component
persistent identity: component2415362
display id: component2415362
version: 1
top level: BBa_K1321342
access: public
definition: https://synbiohub.org/public/igem/BBa_K1321003/1

BBa_K1321337
type: Component
persistent identity: component2415359
display id: component2415359
version: 1
top level: BBa_K1321342
access: public
definition: BBa_K1321337

BBa_B0105
type: Component
persistent identity: component2415361
display id: component2415361
version: 1
top level: BBa_K1321342
access: public
definition: https://synbiohub.org/public/igem/BBa_B0105/1

BBa_K1321003
type: SequenceAnnotation
persistent identity: annotation2415362
display id: annotation2415362
version: 1
top level: BBa_K1321342
location: https://synbiohub.org/public/igem/BBa_K1321342/annotation2415362/range2415362/1
component: BBa_K1321003

https://synbiohub.org/public/igem/BBa_K1321342/annotation2415362/range2415362/1
type: Range
persistent identity: range2415362
display id: range2415362
version: 1
direction: direction/reverse
top level: BBa_K1321342
start: 718
end: 1044
orientation: inline

BBa_B0105
type: SequenceAnnotation
persistent identity: annotation2415361
display id: annotation2415361
version: 1
top level: BBa_K1321342
location: https://synbiohub.org/public/igem/BBa_K1321342/annotation2415361/range2415361/1
component: BBa_B0105

https://synbiohub.org/public/igem/BBa_K1321342/annotation2415361/range2415361/1
type: Range
persistent identity: range2415361
display id: range2415361
version: 1
direction: direction/reverse
top level: BBa_K1321342
start: 712
end: 717
orientation: inline

BBa_K1321337
type: SequenceAnnotation
persistent identity: annotation2415359
display id: annotation2415359
version: 1
top level: BBa_K1321342
location: https://synbiohub.org/public/igem/BBa_K1321342/annotation2415359/range2415359/1
component: BBa_K1321337

https://synbiohub.org/public/igem/BBa_K1321342/annotation2415359/range2415359/1
type: Range
persistent identity: range2415359
display id: range2415359
version: 1
direction: direction/reverse
top level: BBa_K1321342
start: 1
end: 711
orientation: inline

BBa_K1740001
type: ComponentDefinition
persistent identity: BBa K1740001
display id: BBa_K1740001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Dronpa145K ORF (E. coli optimized)
creator: Keisuke Sugahara, Momoko Saegusa
created: 2015-08-19T11:00:00Z
modified: 2016-01-25T11:16:53Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2162_
m user id: 4206
owner id: 25441
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This is test
mutable notes: none
mutable provenance: .
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Dronpa 145K
sequence annotation: 145Asn to 145Lys
sequence annotation: start codon
sequence: https://synbiohub.org/public/igem/BBa_K1740001_sequence/1

Dronpa 145K
type: SequenceAnnotation
persistent identity: annotation2469980
display id: annotation2469980
version: 1
top level: BBa_K1740001
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1740001/annotation2469980/range2469980/1

https://synbiohub.org/public/igem/BBa_K1740001/annotation2469980/range2469980/1
type: Range
persistent identity: range2469980
display id: range2469980
version: 1
direction: direction/forward
top level: BBa_K1740001
start: 1
end: 672
orientation: inline

145Asn to 145Lys
type: SequenceAnnotation
persistent identity: annotation2469984
display id: annotation2469984
version: 1
top level: BBa_K1740001
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K1740001/annotation2469984/range2469984/1

https://synbiohub.org/public/igem/BBa_K1740001/annotation2469984/range2469984/1
type: Range
persistent identity: range2469984
display id: range2469984
version: 1
direction: direction/forward
top level: BBa_K1740001
start: 433
end: 435
orientation: inline

start codon
type: SequenceAnnotation
persistent identity: annotation2469983
display id: annotation2469983
version: 1
top level: BBa_K1740001
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1740001/annotation2469983/range2469983/1

https://synbiohub.org/public/igem/BBa_K1740001/annotation2469983/range2469983/1
type: Range
persistent identity: range2469983
display id: range2469983
version: 1
direction: direction/forward
top level: BBa_K1740001
start: 1
end: 3
orientation: inline

BBa_K1742002
type: ComponentDefinition
persistent identity: BBa K1742002
display id: BBa_K1742002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Dronpa 145N
creator: Iv??n Casas Rodrigo
created: 2015-09-03T11:00:00Z
modified: 2016-01-25T11:27:00Z
discontinued: false
dominant: false
group u list: _2164_
m user id: 4206
owner id: 26598
owning group id: 9
status: status/Planning
bookmark: false
mutable description: A CDS of Dronpa protein with a mutation in the betha-strand 7 in Lys145 for Asn.
mutable notes: It is codon optimized for plants
mutable provenance: Pectinidae sp. sequence found in the following article Lummer, M., Humpert, F., Steuwe, C., Caesar, K., Sch??ttpelz, M., Sauer, M. and Staiger, D. (2011), Reversible Photoswitchable DRONPA-s Monitors Nucleocytoplasmic Transport of an RNA-Binding Protein in Transgenic Plants. Traffic, 12: 693???702. doi: 10.1111/j.1600-0854.2011.01180.x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1742002_sequence/1

sfGFP(Sp)
type: ComponentDefinition
persistent identity: BBa K1930006
display id: BBa_K1930006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: sfGFP(Sp)
creator: Barbora Waclawikova
created: 2016-10-15T11:00:00Z
modified: 2016-10-16T05:16:06Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2397_
m user id: 29748
owner id: 29748
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This part is a reporter sfGFP(Sp) originally optimized for Streptococcus pneumoniae.
mutable notes: sfGFP originally optimized for streptococcus pneumonia because it could be shown that the signal in Bacillus is even brighter than the one from sfGFP(Bs).
mutable provenance: Plasmid pNW
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: sfGFP(Sp)
sequence: https://synbiohub.org/public/igem/BBa_K1930006_sequence/1

sfGFP(Sp)
type: SequenceAnnotation
persistent identity: annotation2513522
display id: annotation2513522
version: 1
top level: sfGFP(Sp)
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1930006/annotation2513522/range2513522/1

https://synbiohub.org/public/igem/BBa_K1930006/annotation2513522/range2513522/1
type: Range
persistent identity: range2513522
display id: range2513522
version: 1
direction: direction/forward
top level: sfGFP(Sp)
start: 1
end: 722
orientation: inline

BBa_K1899003
type: ComponentDefinition
persistent identity: BBa K1899003
display id: BBa_K1899003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Superfolder GFP
creator: iGEM2016_HKUST
created: 2016-08-27T11:00:00Z
modified: 2016-10-15T04:03:09Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2365_
m user id: 26069
owner id: 26069
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Variant of GFP that folds robustly even when fused to poorly folded proteins. Waldo and other research groups has reported the engineering of a superfolder GFP (sfGFP) that showed increased resistance to denaturation, improved folding kinetics, and increased resistance to aggregation during refolding (Pedelacq et al., 2006; Andrews et al., 2007; Fisher and DeLisa, 2008). sfGFP has proven to be very useful as a scaffold for improved protein detection and tagging both in vivo and in vitro using self-assembled sfGFP fragments (Cabantous et al., 2005b; Cabantous and Waldo, 2006). Furthermore, sfGFP fusions are more soluble than conventional GFP fusions (Wu et al., 2009).
mutable notes: nil
mutable provenance: Waldo lab
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: scar
sequence annotation: BBa_B0032
sequence annotation: BBa_J61048
sequence annotation: sfGFP
sequence: https://synbiohub.org/public/igem/BBa_K1899003_sequence/1

scar
type: SequenceAnnotation
persistent identity: annotation2481804
display id: annotation2481804
version: 1
top level: BBa_K1899003
role: feature/scar
location: https://synbiohub.org/public/igem/BBa_K1899003/annotation2481804/range2481804/1

https://synbiohub.org/public/igem/BBa_K1899003/annotation2481804/range2481804/1
type: Range
persistent identity: range2481804
display id: range2481804
version: 1
direction: direction/forward
top level: BBa_K1899003
start: 13
end: 19
orientation: inline

scar
type: SequenceAnnotation
persistent identity: annotation2481806
display id: annotation2481806
version: 1
top level: BBa_K1899003
role: feature/scar
location: https://synbiohub.org/public/igem/BBa_K1899003/annotation2481806/range2481806/1

https://synbiohub.org/public/igem/BBa_K1899003/annotation2481806/range2481806/1
type: Range
persistent identity: range2481806
display id: range2481806
version: 1
direction: direction/forward
top level: BBa_K1899003
start: 734
end: 741
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation2481802
display id: annotation2481802
version: 1
top level: BBa_K1899003
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1899003/annotation2481802/range2481802/1

https://synbiohub.org/public/igem/BBa_K1899003/annotation2481802/range2481802/1
type: Range
persistent identity: range2481802
display id: range2481802
version: 1
direction: direction/forward
top level: BBa_K1899003
start: 1
end: 12
orientation: inline

BBa_J61048
type: SequenceAnnotation
persistent identity: annotation2481805
display id: annotation2481805
version: 1
top level: BBa_K1899003
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1899003/annotation2481805/range2481805/1

https://synbiohub.org/public/igem/BBa_K1899003/annotation2481805/range2481805/1
type: Range
persistent identity: range2481805
display id: range2481805
version: 1
direction: direction/forward
top level: BBa_K1899003
start: 742
end: 854
orientation: inline

sfGFP
type: SequenceAnnotation
persistent identity: annotation2481803
display id: annotation2481803
version: 1
top level: BBa_K1899003
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1899003/annotation2481803/range2481803/1

https://synbiohub.org/public/igem/BBa_K1899003/annotation2481803/range2481803/1
type: Range
persistent identity: range2481803
display id: range2481803
version: 1
direction: direction/forward
top level: BBa_K1899003
start: 20
end: 733
orientation: inline

BBa_K1680020
type: ComponentDefinition
persistent identity: BBa K1680020
display id: BBa_K1680020
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Cre-dronpa fusion
creator: Nicolai von Kuegelgen
created: 2015-09-16T11:00:00Z
modified: 2016-01-26T10:16:19Z
discontinued: false
dominant: false
group u list: _2098_
m user id: 4206
owner id: 26366
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Protein coding region for a fusion construct of Cre Recombinase and 145N Dronpa with 15 amino acid linker. Oligomerisation of Dronpa should stop Cre from accessing DNA in the nucleus thereby inhibiting it until light deactivation of dronpa.
mutable notes: 3A assembly using RFC25
mutable provenance: gBlock design by Team Tuebingen
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: K1680003
sequence annotation: K1680006
sequence annotation: K1680007
sequence: https://synbiohub.org/public/igem/BBa_K1680020_sequence/1

K1680003
type: SequenceAnnotation
persistent identity: annotation2477365
display id: annotation2477365
version: 1
top level: BBa_K1680020
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1680020/annotation2477365/range2477365/1

https://synbiohub.org/public/igem/BBa_K1680020/annotation2477365/range2477365/1
type: Range
persistent identity: range2477365
display id: range2477365
version: 1
direction: direction/forward
top level: BBa_K1680020
start: 1
end: 39
orientation: inline

K1680006
type: SequenceAnnotation
persistent identity: annotation2477364
display id: annotation2477364
version: 1
top level: BBa_K1680020
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1680020/annotation2477364/range2477364/1

https://synbiohub.org/public/igem/BBa_K1680020/annotation2477364/range2477364/1
type: Range
persistent identity: range2477364
display id: range2477364
version: 1
direction: direction/forward
top level: BBa_K1680020
start: 1114
end: 1782
orientation: inline

K1680003
type: SequenceAnnotation
persistent identity: annotation2477366
display id: annotation2477366
version: 1
top level: BBa_K1680020
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1680020/annotation2477366/range2477366/1

https://synbiohub.org/public/igem/BBa_K1680020/annotation2477366/range2477366/1
type: Range
persistent identity: range2477366
display id: range2477366
version: 1
direction: direction/forward
top level: BBa_K1680020
start: 1089
end: 1113
orientation: inline

K1680007
type: SequenceAnnotation
persistent identity: annotation2477363
display id: annotation2477363
version: 1
top level: BBa_K1680020
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1680020/annotation2477363/range2477363/1

https://synbiohub.org/public/igem/BBa_K1680020/annotation2477363/range2477363/1
type: Range
persistent identity: range2477363
display id: range2477363
version: 1
direction: direction/forward
top level: BBa_K1680020
start: 40
end: 1068
orientation: inline

BBa_K648013
type: ComponentDefinition
persistent identity: BBa K648013
display id: BBa_K648013
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: GFP with Standard 25 Prefix/Suffix
creator: Jim Rose
created: 2011-07-03T11:00:00Z
modified: 2016-01-25T02:32:21Z
discontinued: false
dominant: false
group u list: _825_
m user id: 4206
owner id: 9871
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This is the standard GFP protein reporter (E0040) cloned with the prefix/suffix required for standard 25 assembly. It can easily be used to create fusion proteins through this method.
mutable notes: This part contains the AgeI and NgoMIV sites as part of it's prefix/suffix that allows it to be used in standard 25 assembly. It was synthesized through PCR oligo synthesis methods using the following primers: Forward (56.88 degrees) TATTGAATTCGCGGCCGCTTCTAGATGGCCGGCCGTAAAGGAGAAGAACTTTTC Reverse (56.95 degrees) AATACTGCAGCGGCCGCTACTAGTATTAACCGGTCTTGTCGTCATCATCTTTATAAT
mutable provenance: This part is similar to the part BBa_E0040 commonly used as a reporter.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: misc
sequence: https://synbiohub.org/public/igem/BBa_K648013_sequence/1

misc
type: SequenceAnnotation
persistent identity: annotation2122827
display id: annotation2122827
version: 1
top level: BBa_K648013
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K648013/annotation2122827/range2122827/1

https://synbiohub.org/public/igem/BBa_K648013/annotation2122827/range2122827/1
type: Range
persistent identity: range2122827
display id: range2122827
version: 1
direction: direction/forward
top level: BBa_K648013
start: 1
end: 735
orientation: inline

BBa_K1980012
type: ComponentDefinition
persistent identity: BBa K1980012
display id: BBa_K1980012
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pCopA MymT sfGFP with divergent CueR
creator: Sam Garforth
created: 2016-10-10T11:00:00Z
modified: 2016-10-23T07:47:17Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2447_
m user id: 0
m user id: 29607
owner id: 29607
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: pCopA MymT sfGFP with plasmid constitutively expressed CueR
mutable notes: dsjsdgydahjah
mutable provenance: E. coli TB
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_K1980003
component: BBa_K1980006
sequence annotation: BBa_K1980006
sequence annotation: BBa_K1980003
sequence: https://synbiohub.org/public/igem/BBa_K1980012_sequence/1

BBa_K1980003
type: Component
persistent identity: component2493046
display id: component2493046
version: 1
top level: BBa_K1980012
access: public
definition: MymTsfGFP

BBa_K1980006
type: Component
persistent identity: component2493045
display id: component2493045
version: 1
top level: BBa_K1980012
access: public
definition: https://synbiohub.org/public/igem/BBa_K1980006/1

BBa_K1980006
type: SequenceAnnotation
persistent identity: annotation2493045
display id: annotation2493045
version: 1
top level: BBa_K1980012
location: https://synbiohub.org/public/igem/BBa_K1980012/annotation2493045/range2493045/1
component: BBa_K1980006

https://synbiohub.org/public/igem/BBa_K1980012/annotation2493045/range2493045/1
type: Range
persistent identity: range2493045
display id: range2493045
version: 1
direction: direction/reverse
top level: BBa_K1980012
start: 1
end: 574
orientation: inline

BBa_K1980003
type: SequenceAnnotation
persistent identity: annotation2493046
display id: annotation2493046
version: 1
top level: BBa_K1980012
location: https://synbiohub.org/public/igem/BBa_K1980012/annotation2493046/range2493046/1
component: BBa_K1980003

https://synbiohub.org/public/igem/BBa_K1980012/annotation2493046/range2493046/1
type: Range
persistent identity: range2493046
display id: range2493046
version: 1
direction: direction/reverse
top level: BBa_K1980012
start: 581
end: 1492
orientation: inline

BBa_K1980005
type: ComponentDefinition
persistent identity: BBa K1980005
display id: BBa_K1980005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pCopA sfGFP
creator: Sam Garforth
created: 2016-10-10T11:00:00Z
modified: 2016-10-23T04:46:35Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2447_
m user id: 29607
owner id: 29607
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This is the copper sensitive promoter pCopA (controlled by CueR) in front of an RBS and a sfGFP reporter
mutable notes: Codon optised to E. coli.
mutable provenance: E. coli genome.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: Stop
sequence annotation: CueR binding
sequence annotation: RBS
sequence annotation: Hexahis
sequence annotation: pCopA
sequence annotation: sfGFP
sequence: https://synbiohub.org/public/igem/BBa_K1980005_sequence/1

Stop
type: SequenceAnnotation
persistent identity: annotation2528243
display id: annotation2528243
version: 1
top level: BBa_K1980005
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1980005/annotation2528243/range2528243/1

https://synbiohub.org/public/igem/BBa_K1980005/annotation2528243/range2528243/1
type: Range
persistent identity: range2528243
display id: range2528243
version: 1
direction: direction/forward
top level: BBa_K1980005
start: 805
end: 810
orientation: inline

CueR binding
type: SequenceAnnotation
persistent identity: annotation2528664
display id: annotation2528664
version: 1
top level: BBa_K1980005
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1980005/annotation2528664/range2528664/1

https://synbiohub.org/public/igem/BBa_K1980005/annotation2528664/range2528664/1
type: Range
persistent identity: range2528664
display id: range2528664
version: 1
direction: direction/forward
top level: BBa_K1980005
start: 19
end: 37
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2528240
display id: annotation2528240
version: 1
top level: BBa_K1980005
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1980005/annotation2528240/range2528240/1

https://synbiohub.org/public/igem/BBa_K1980005/annotation2528240/range2528240/1
type: Range
persistent identity: range2528240
display id: range2528240
version: 1
direction: direction/forward
top level: BBa_K1980005
start: 57
end: 62
orientation: inline

Hexahis
type: SequenceAnnotation
persistent identity: annotation2528242
display id: annotation2528242
version: 1
top level: BBa_K1980005
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1980005/annotation2528242/range2528242/1

https://synbiohub.org/public/igem/BBa_K1980005/annotation2528242/range2528242/1
type: Range
persistent identity: range2528242
display id: range2528242
version: 1
direction: direction/forward
top level: BBa_K1980005
start: 787
end: 804
orientation: inline

pCopA
type: SequenceAnnotation
persistent identity: annotation2528239
display id: annotation2528239
version: 1
top level: BBa_K1980005
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1980005/annotation2528239/range2528239/1

https://synbiohub.org/public/igem/BBa_K1980005/annotation2528239/range2528239/1
type: Range
persistent identity: range2528239
display id: range2528239
version: 1
direction: direction/forward
top level: BBa_K1980005
start: 1
end: 51
orientation: inline

sfGFP
type: SequenceAnnotation
persistent identity: annotation2528241
display id: annotation2528241
version: 1
top level: BBa_K1980005
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1980005/annotation2528241/range2528241/1

https://synbiohub.org/public/igem/BBa_K1980005/annotation2528241/range2528241/1
type: Range
persistent identity: range2528241
display id: range2528241
version: 1
direction: direction/forward
top level: BBa_K1980005
start: 73
end: 786
orientation: inline

GFPLVA
type: ComponentDefinition
persistent identity: BBa K411207
display id: BBa_K411207
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Green Fluorescent Protein with LVA tag (GFPLVA)
creator: KO KUEI YUEH
created: 2010-10-21T11:00:00Z
modified: 2016-01-28T12:22:52Z
discontinued: false
dominant: true
experience: experience/None
group u list: _526_
m user id: 4206
owner id: 6665
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: GFP with LVA tag can be degrage by SspB
mutable notes: GFP with LVA-tag can be degrade by speedy degrager and has a short half-life
mutable provenance: Biobrick E0040
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: GFP
sequence annotation: LVA tag
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_K411207_sequence/1

GFP
type: SequenceAnnotation
persistent identity: annotation2092218
display id: annotation2092218
version: 1
top level: GFPLVA
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K411207/annotation2092218/range2092218/1

https://synbiohub.org/public/igem/BBa_K411207/annotation2092218/range2092218/1
type: Range
persistent identity: range2092218
display id: range2092218
version: 1
direction: direction/forward
top level: GFPLVA
start: 1
end: 714
orientation: inline

LVA tag
type: SequenceAnnotation
persistent identity: annotation2092217
display id: annotation2092217
version: 1
top level: GFPLVA
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K411207/annotation2092217/range2092217/1

https://synbiohub.org/public/igem/BBa_K411207/annotation2092217/range2092217/1
type: Range
persistent identity: range2092217
display id: range2092217
version: 1
direction: direction/forward
top level: GFPLVA
start: 715
end: 747
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2101796
display id: annotation2101796
version: 1
top level: GFPLVA
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K411207/annotation2101796/range2101796/1

https://synbiohub.org/public/igem/BBa_K411207/annotation2101796/range2101796/1
type: Range
persistent identity: range2101796
display id: range2101796
version: 1
direction: direction/forward
top level: GFPLVA
start: 748
end: 753
orientation: inline

BBa_K1789003
type: ComponentDefinition
persistent identity: BBa K1789003
display id: BBa_K1789003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: GFP1(with termination codon)
creator: Xinyuan Qiu
created: 2015-09-13T11:00:00Z
modified: 2016-01-25T01:04:29Z
discontinued: false
dominant: false
group u list: _2214_
m user id: 4206
owner id: 21134
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This part is the Amino Half of GFP with termination codon
mutable notes: Because of the need of our project, we added a termination codon when designing the PCR.
mutable provenance: This part is gained from part BBa_I715019. we added the termination codon when designing the PCR.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: termination codon
sequence annotation: GFP1
sequence: https://synbiohub.org/public/igem/BBa_K1789003_sequence/1

termination codon
type: SequenceAnnotation
persistent identity: annotation2454066
display id: annotation2454066
version: 1
top level: BBa_K1789003
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1789003/annotation2454066/range2454066/1

https://synbiohub.org/public/igem/BBa_K1789003/annotation2454066/range2454066/1
type: Range
persistent identity: range2454066
display id: range2454066
version: 1
direction: direction/forward
top level: BBa_K1789003
start: 472
end: 474
orientation: inline

GFP1
type: SequenceAnnotation
persistent identity: annotation2454065
display id: annotation2454065
version: 1
top level: BBa_K1789003
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1789003/annotation2454065/range2454065/1

https://synbiohub.org/public/igem/BBa_K1789003/annotation2454065/range2454065/1
type: Range
persistent identity: range2454065
display id: range2454065
version: 1
direction: direction/forward
top level: BBa_K1789003
start: 1
end: 471
orientation: inline

BBa_K1680023
type: ComponentDefinition
persistent identity: BBa K1680023
display id: BBa_K1680023
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Dronpa caged Cre with NLS
creator: Nicolai von Kuegelgen
created: 2015-09-16T11:00:00Z
modified: 2016-01-26T10:42:40Z
discontinued: false
dominant: false
group u list: _2098_
m user id: 4206
owner id: 26366
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Protein coding region for a fusion protein of Cre recombinase (BBa_K1680007) that is C- & N-terminal flanked with 145N Dronpa (BBa_K1680006) using a 12 AA linker as spacer (BBa_K1680002). The construct has a N-terminal NLS sequence (BBa_K1680004). In the expressed construct the two dronpa domains should form multimerize leading to an inactive Cre recombinase that is reversibly activatable through dronpa illumination (see Zhou et al 2012).
mutable notes: 3A assembly using RFC25
mutable provenance: gBlock design by Team Tuebingen
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: K1680007
sequence annotation: K1680004
sequence annotation: K1680006
sequence annotation: K1680002
sequence: https://synbiohub.org/public/igem/BBa_K1680023_sequence/1

K1680007
type: SequenceAnnotation
persistent identity: annotation2477382
display id: annotation2477382
version: 1
top level: BBa_K1680023
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1680023/annotation2477382/range2477382/1

https://synbiohub.org/public/igem/BBa_K1680023/annotation2477382/range2477382/1
type: Range
persistent identity: range2477382
display id: range2477382
version: 1
direction: direction/forward
top level: BBa_K1680023
start: 736
end: 1764
orientation: inline

K1680004
type: SequenceAnnotation
persistent identity: annotation2477379
display id: annotation2477379
version: 1
top level: BBa_K1680023
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1680023/annotation2477379/range2477379/1

https://synbiohub.org/public/igem/BBa_K1680023/annotation2477379/range2477379/1
type: Range
persistent identity: range2477379
display id: range2477379
version: 1
direction: direction/forward
top level: BBa_K1680023
start: 1
end: 24
orientation: inline

K1680006
type: SequenceAnnotation
persistent identity: annotation2477380
display id: annotation2477380
version: 1
top level: BBa_K1680023
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1680023/annotation2477380/range2477380/1

https://synbiohub.org/public/igem/BBa_K1680023/annotation2477380/range2477380/1
type: Range
persistent identity: range2477380
display id: range2477380
version: 1
direction: direction/forward
top level: BBa_K1680023
start: 31
end: 699
orientation: inline

K1680002
type: SequenceAnnotation
persistent identity: annotation2477384
display id: annotation2477384
version: 1
top level: BBa_K1680023
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1680023/annotation2477384/range2477384/1

https://synbiohub.org/public/igem/BBa_K1680023/annotation2477384/range2477384/1
type: Range
persistent identity: range2477384
display id: range2477384
version: 1
direction: direction/forward
top level: BBa_K1680023
start: 1765
end: 1800
orientation: inline

K1680002
type: SequenceAnnotation
persistent identity: annotation2477383
display id: annotation2477383
version: 1
top level: BBa_K1680023
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1680023/annotation2477383/range2477383/1

https://synbiohub.org/public/igem/BBa_K1680023/annotation2477383/range2477383/1
type: Range
persistent identity: range2477383
display id: range2477383
version: 1
direction: direction/forward
top level: BBa_K1680023
start: 700
end: 735
orientation: inline

K1680006
type: SequenceAnnotation
persistent identity: annotation2477381
display id: annotation2477381
version: 1
top level: BBa_K1680023
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1680023/annotation2477381/range2477381/1

https://synbiohub.org/public/igem/BBa_K1680023/annotation2477381/range2477381/1
type: Range
persistent identity: range2477381
display id: range2477381
version: 1
direction: direction/forward
top level: BBa_K1680023
start: 1801
end: 2469
orientation: inline

BBa_J97001
type: ComponentDefinition
persistent identity: BBa J97001
display id: BBa_J97001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: P-Free JuniperGFP (Green Fluorescent Protein)
creator: Drew Endy
created: 2014-02-18T12:00:00Z
modified: 2015-05-08T01:08:32Z
discontinued: false
dominant: false
experience: experience/None
group u list: _41_1_
m user id: 0
owner id: 747
owning group id: 63
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This part was created by DNA 2.0 as part of their IP-Free series of fluorescent and chromogenic proteins. It is available to use under the BioBrick Public Agreement. Excitation 508nm, Emission 521nm Molecular Weight: 26.6kDA Length: 237aa
mutable notes: This sequence is fully synthetic, (non-Aequorea) and is codon optimized for expression in E. coli.
mutable provenance: This synthetic part was created through a gene engineering and synthesis project at DNA 2.0.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence: https://synbiohub.org/public/igem/BBa_J97001_sequence/1

Csp1sfGFP
type: ComponentDefinition
persistent identity: BBa K1980001
display id: BBa_K1980001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TAT Copper Storage Protein 1 sfGFP
creator: Sam Garforth
created: 2016-10-10T11:00:00Z
modified: 2016-10-12T02:35:29Z
discontinued: false
dominant: false
experience: experience/Issues
group u list: _2447_
m user id: 29607
owner id: 29607
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Copper Storage protein 1 (Csp1) is a tetrameric copper storage protein found in the periplasm of Methylosinus trichosporium OB3b. We investigated whether this part could act as a copper chelator when expressed in E. coli. We modified the protein by adding a TAT signal peptide from the E. coli enzyme CueO in place of the native TAT sequence and added a c terminal sfGFP fluorescence protein with a C terminal his tag in an attempt to direct the protein to the periplasm. The Csp1 and sfGFP are separated by a short hydrophilic, flexible linker.
mutable notes: Codon optimised for E. coli. linker added in order to allow the Csp1 and sfGFP to ideally fold separately. Hexahistidine purification tag on c-terminus to maintain TAT sequence.
mutable provenance: The source organism for the main protein sequence is Methylosinus trichosporium OB3b. The TAT sequence originate from E. coli multi copper oxidase enzyme CueO. We ordered it as codon optimised DNA from IDT.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: TAT signal peptide
sequence annotation: linker
sequence annotation: sfGFP
sequence annotation: Csp1
sequence annotation: Hexahistidine
sequence annotation: Double stop
sequence: https://synbiohub.org/public/igem/BBa_K1980001_sequence/1

TAT signal peptide
type: SequenceAnnotation
persistent identity: annotation2495227
display id: annotation2495227
version: 1
top level: Csp1sfGFP
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1980001/annotation2495227/range2495227/1

https://synbiohub.org/public/igem/BBa_K1980001/annotation2495227/range2495227/1
type: Range
persistent identity: range2495227
display id: range2495227
version: 1
direction: direction/forward
top level: Csp1sfGFP
start: 1
end: 84
orientation: inline

linker
type: SequenceAnnotation
persistent identity: annotation2495229
display id: annotation2495229
version: 1
top level: Csp1sfGFP
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1980001/annotation2495229/range2495229/1

https://synbiohub.org/public/igem/BBa_K1980001/annotation2495229/range2495229/1
type: Range
persistent identity: range2495229
display id: range2495229
version: 1
direction: direction/forward
top level: Csp1sfGFP
start: 451
end: 468
orientation: inline

sfGFP
type: SequenceAnnotation
persistent identity: annotation2495230
display id: annotation2495230
version: 1
top level: Csp1sfGFP
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1980001/annotation2495230/range2495230/1

https://synbiohub.org/public/igem/BBa_K1980001/annotation2495230/range2495230/1
type: Range
persistent identity: range2495230
display id: range2495230
version: 1
direction: direction/forward
top level: Csp1sfGFP
start: 469
end: 1179
orientation: inline

Csp1
type: SequenceAnnotation
persistent identity: annotation2495228
display id: annotation2495228
version: 1
top level: Csp1sfGFP
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1980001/annotation2495228/range2495228/1

https://synbiohub.org/public/igem/BBa_K1980001/annotation2495228/range2495228/1
type: Range
persistent identity: range2495228
display id: range2495228
version: 1
direction: direction/forward
top level: Csp1sfGFP
start: 85
end: 450
orientation: inline

Hexahistidine
type: SequenceAnnotation
persistent identity: annotation2495231
display id: annotation2495231
version: 1
top level: Csp1sfGFP
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1980001/annotation2495231/range2495231/1

https://synbiohub.org/public/igem/BBa_K1980001/annotation2495231/range2495231/1
type: Range
persistent identity: range2495231
display id: range2495231
version: 1
direction: direction/forward
top level: Csp1sfGFP
start: 1180
end: 1197
orientation: inline

Double stop
type: SequenceAnnotation
persistent identity: annotation2495238
display id: annotation2495238
version: 1
top level: Csp1sfGFP
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1980001/annotation2495238/range2495238/1

https://synbiohub.org/public/igem/BBa_K1980001/annotation2495238/range2495238/1
type: Range
persistent identity: range2495238
display id: range2495238
version: 1
direction: direction/forward
top level: Csp1sfGFP
start: 1198
end: 1203
orientation: inline