cds_reporter_gfp
1
//cds/reporter/gfp
BBa_K1321337
1
BBa_K1321337
sfGFP in Freiburg format (RFC 25)
Chris N Micklem
2014-10-07T11:00:00Z
2016-01-26T02:10:16Z
false
false
_1696_
4206
20830
9
In stock
true
sfGFP in Freiburg format (RFC 25) to allow for easy use in fusion proteins.
OEPCR
Made from existing part.
false
annotation2417826
1
sfGFP
range2417826
1
1
711
BBa_K1909014
1
BBa_K1909014
Omp Promoter / eYFP
Daniel Wedemeyer
2016-10-13T11:00:00Z
2016-10-14T12:58:07Z
false
false
_2375_
29525
29525
9
false
Coming Soon
Coming Soon
Composite Part
false
component2508857
1
BBa_R0082
component2508867
1
BBa_E0430
annotation2508867
1
BBa_E0430
range2508867
1
117
994
annotation2508857
1
BBa_R0082
range2508857
1
1
108
BBa_K1399016
1
BBa_K1399016
Plac-GFP(LAA)
Anna Stikane
2014-09-18T11:00:00Z
2015-05-08T01:10:16Z
false
false
_1777_
0
22477
9
In stock
true
Lactose/IPTG inducible promoter with GFP reporter tagged with AAV-ssrA degradation tag followed by terminator.
The tagged GFP is actively degraded within cell, thus provides better temporal resolution of green fluorescence and promoter activity.
Part was assembled using BrickClip assembly (BBF RFC104) using other biobrick parts as templates. BrickClip assembly is a special case of more general Paperclip assembly method.[1]
other biobricks
false
component2384166
1
BBa_B0015
component2384159
1
BBa_K1399006
component2384153
1
BBa_B0034
component2384145
1
BBa_R0010
annotation2384153
1
BBa_B0034
range2384153
1
209
220
annotation2384145
1
BBa_R0010
range2384145
1
1
200
annotation2384159
1
BBa_K1399006
range2384159
1
227
979
annotation2384166
1
BBa_B0015
range2384166
1
988
1116
BBa_K1399015
1
BBa_K1399015
Plac-GFP(LVA)
Anna Stikane
2014-09-18T11:00:00Z
2015-05-08T01:10:16Z
false
false
_1777_
0
22477
9
In stock
true
Lactose/IPTG inducible promoter with GFP reporter tagged with AAV-ssrA degradation tag followed by terminator.
The tagged GFP is actively degraded within cell, thus provides better temporal resolution of green fluorescence and promoter activity.
Part was assembled using BrickClip assembly (BBF RFC104) using other biobrick parts as templates. BrickClip assembly is a special case of more general Paperclip assembly method.[1]
other biobricks
false
component2384064
1
BBa_R0010
component2384078
1
BBa_K1399004
component2384072
1
BBa_B0034
component2384085
1
BBa_B0015
annotation2384064
1
BBa_R0010
range2384064
1
1
200
annotation2384078
1
BBa_K1399004
range2384078
1
227
979
annotation2384085
1
BBa_B0015
range2384085
1
988
1116
annotation2384072
1
BBa_B0034
range2384072
1
209
220
BBa_K1486028
1
BBa_K1486028
Yeast optimized sfGFP N-terminus (1-214)
EPFL iGem team 2014
2014-09-26T11:00:00Z
2015-05-08T01:10:42Z
false
false
_1866_
0
21042
9
Not in stock
false
split N term of sfGFP To edit
To edit
To edit
false
annotation2417081
1
yeast optimized sfGFP N-terminus
range2417081
1
1
642
BBa_K1732022
1
BBa_K1732022
EGFPCO-His
Donna Lee
2015-09-17T11:00:00Z
2016-01-21T02:51:07Z
false
false
_2154_
4206
27195
9
false
EGFP (BBa_1491002) with 6X-His tag for easier protein purification. Original sequence is codon optimized by IDT Codon Optimized Tool for E.coli.
Codon optimized for E.coli and His tag allows for easier protein purification.
BBa_1491002
false
annotation2469121
1
6X-His
range2469121
1
717
733
annotation2469080
1
EGFPCO
range2469080
1
1
716
BBa_K1680021
1
BBa_K1680021
Dronpa caged Cre with NLS
Nicolai von Kuegelgen
2015-09-16T11:00:00Z
2016-01-26T01:27:03Z
false
false
_2098_
4206
26366
9
false
Protein coding region for a fusion protein of Cre recombinase (BBa_K1680007) that is C- & N-terminal flanked with 145N Dronpa (BBa_K1680006) using a 6 AA linker as spacer (BBa_K1680000). The construct has a N-terminal NLS sequence (BBa_K1680004). In the expressed construct the two dronpa domains should form multimerize leading to an inactive Cre recombinase that is reversibly activatable through dronpa illumination (see Zhou et al 2012).
3A Assembly using RFC25
gBlock design by Team Tuebingen
false
annotation2477378
1
Dronpa
range2477378
1
1765
2433
annotation2477377
1
linker
range2477377
1
1747
1764
annotation2477375
1
linker
range2477375
1
700
717
annotation2477376
1
Cre recombinase
range2477376
1
718
1746
annotation2477373
1
SV40 NLS
range2477373
1
4
24
annotation2477374
1
Dronpa
range2477374
1
31
699
BBa_K1789003
1
BBa_K1789003
GFP1(with termination codon)
Xinyuan Qiu
2015-09-13T11:00:00Z
2016-01-25T01:04:29Z
false
false
_2214_
4206
21134
9
false
This part is the Amino Half of GFP with termination codon
Because of the need of our project, we added a termination codon when designing the PCR.
This part is gained from part BBa_I715019. we added the termination codon when designing the PCR.
false
annotation2454066
1
termination codon
range2454066
1
472
474
annotation2454065
1
GFP1
range2454065
1
1
471
BBa_K1740000
1
BBa_K1740000
Dronpa 145N ORF (E. coli optimized)
Yukina Tanimura,Keisuke Sugahara,
2015-08-19T11:00:00Z
2016-01-25T11:17:08Z
false
false
_2162_
4206
25441
9
false
This is test
Non
synthesis DNA
false
annotation2444413
1
145Lys to 145Asn
range2444413
1
433
435
annotation2444414
1
Dronpa 145N
range2444414
1
1
672
annotation2444415
1
strat codon
range2444415
1
1
3
BBa_K1980010
1
BBa_K1980010
pCopA TAT Csp1 sfGFP with divergent CueR
Sam Garforth
2016-10-10T11:00:00Z
2016-10-24T08:12:51Z
false
false
_2447_
29607
29607
9
false
pCopA TAT Csp1 sfGFP with plasmid constitutively expressed CueR
aahahahah
E. coli, methanoba trcol OB3b
false
component2493041
1
BBa_K1980006
component2493042
1
BBa_K1980001
annotation2493042
1
BBa_K1980001
range2493042
1
581
1783
annotation2493041
1
BBa_K1980006
range2493041
1
1
574
BBa_K1399023
1
BBa_K1399023
Ptet-GFP(DAS)
Anna Stikane
2014-09-18T11:00:00Z
2015-05-08T01:10:16Z
false
false
_1777_
0
22477
9
In stock
false
Tetracycline/aTc inducible promoter with GFP reporter tagged with DAS-ssrA degradation tag followed by terminator.
The tagged GFP is actively degraded within cell, thus provides better temporal resolution of green fluorescence and promoter activity.Presence of SspB is crucial for effective protein degradation.
Part was assembled using BrickClip assembly (BBF RFC104) using other biobrick parts as templates. BrickClip assembly is a special case of more general Paperclip assembly method.[1]
other biobricks
false
component2384299
1
BBa_R0040
component2384318
1
BBa_B0015
component2384305
1
BBa_B0034
component2384311
1
BBa_K1399008
annotation2384311
1
BBa_K1399008
range2384311
1
81
833
annotation2384305
1
BBa_B0034
range2384305
1
63
74
annotation2384299
1
BBa_R0040
range2384299
1
1
54
annotation2384318
1
BBa_B0015
range2384318
1
842
970
BBa_K1399017
1
BBa_K1399017
Plac-GFP(DAS+2)
Anna Stikane
2014-09-18T11:00:00Z
2015-05-08T01:10:16Z
false
false
_1777_
0
22477
9
In stock
true
Lactose/IPTG inducible promoter with GFP reporter tagged with NYADAS-ssrA degradation tag followed by terminator.
The tagged GFP is actively degraded within cell, thus provides better temporal resolution of green fluorescence and promoter activity.
Part was assembled using BrickClip assembly (BBF RFC104) using other biobrick parts as templates. BrickClip assembly is a special case of more general Paperclip assembly method.[1]
other biobricks
false
component2384175
1
BBa_R0010
component2384189
1
BBa_K1399007
component2384183
1
BBa_B0034
component2384196
1
BBa_B0015
annotation2384189
1
BBa_K1399007
range2384189
1
227
985
annotation2384183
1
BBa_B0034
range2384183
1
209
220
annotation2384196
1
BBa_B0015
range2384196
1
994
1122
annotation2384175
1
BBa_R0010
range2384175
1
1
200
BBa_K1486005
1
BBa_K1486005
Arabinose promoter + sfGFP-CpxR [Cterm]
iGem EPFL 2014
2014-08-12T11:00:00Z
2015-05-08T01:10:42Z
false
false
_1866_
0
21042
9
It's complicated
false
This part is composed of an arabinose-inducible promoter for superfolded GFP attached by a linker to CpxR (on the C terminus of CpxR).
We had to test both attachments, N and C terminus of the CpxR.
The arabinose promoter and the sfGFP were obtained from the registry, while the CpxR was obtained from the E coli genome.
false
component2382093
1
BBa_I746916
component2382084
1
BBa_B0034
component2382089
1
BBa_K1486003
component2382082
1
BBa_I0500
component2382088
1
BBa_K1486000
annotation2382089
1
BBa_K1486003
range2382089
1
1925
1948
annotation2382093
1
BBa_I746916
range2382093
1
1949
2668
annotation2382082
1
BBa_I0500
range2382082
1
1
1210
annotation2382088
1
BBa_K1486000
range2382088
1
1223
1924
annotation2382084
1
BBa_B0034
range2382084
1
1211
1222
BBa_K1980012
1
BBa_K1980012
pCopA MymT sfGFP with divergent CueR
Sam Garforth
2016-10-10T11:00:00Z
2016-10-23T07:47:17Z
false
false
_2447_
0
29607
29607
9
It's complicated
true
pCopA MymT sfGFP with plasmid constitutively expressed CueR
dsjsdgydahjah
E. coli TB
false
component2493046
1
BBa_K1980003
component2493045
1
BBa_K1980006
annotation2493045
1
BBa_K1980006
range2493045
1
1
574
annotation2493046
1
BBa_K1980003
range2493046
1
581
1492
BBa_K1740001
1
BBa_K1740001
Dronpa145K ORF (E. coli optimized)
Keisuke Sugahara, Momoko Saegusa
2015-08-19T11:00:00Z
2016-01-25T11:16:53Z
false
false
_2162_
4206
25441
9
false
This is test
none
.
false
annotation2469983
1
start codon
range2469983
1
1
3
annotation2469984
1
145Asn to 145Lys
range2469984
1
433
435
annotation2469980
1
Dronpa 145K
range2469980
1
1
672
BBa_J52021
1
BBa_J52021
dnTraf6-linker-GFP
Matej Skocaj
2006-10-13T11:00:00Z
2016-01-26T09:38:59Z
false
false
_80_
0
4206
857
80
Released HQ 2013
In stock
false
Part is composed of TIR domain of Traf6 and GFP linked with 6 aminoacids long linker. GFP serves as reporter. For usage in eukaryotes.
None.
dnTraf6- pDeNy
GFP-
true
annotation1903261
1
GFP
range1903261
1
733
1446
annotation1903262
1
dnTraf6-linker-GFP
range1903262
1
1
1446
annotation1903260
1
linker
range1903260
1
715
732
annotation1903259
1
dnTraf6
range1903259
1
1
714
BBa_K106028
1
BBa_K106028
GFP, AarI AB part
Andrej Ondracka
2008-10-24T11:00:00Z
2016-01-26T09:49:32Z
false
false
_226_
4206
3416
9
It's complicated
true
This is coding sequence of GFP (yeast optimized codons).
.
.
true
BBa_K1486002
1
BBa_K1486002
Arabinose promoter + sfGFP CpxR (Nterm)
iGem EPFL 2014
2014-08-11T11:00:00Z
2015-05-08T01:10:42Z
false
false
_1866_
0
21042
9
It's complicated
false
This part is composed of an arabinose-inducible promoter for superfolded GFP attached by a linker to CpxR (on the N terminus of CpxR).
We had to test both attachments, N and C terminus of the CpxR.
The arabinose promoter and the sfGFP were obtained from the registry, while the CpxR was obtained from the E coli genome.
false
component2382057
1
BBa_I0500
component2382064
1
BBa_K1486003
component2382063
1
BBa_I746916
component2382068
1
BBa_K1486000
component2382059
1
BBa_B0034
annotation2382057
1
BBa_I0500
range2382057
1
1
1210
annotation2382064
1
BBa_K1486003
range2382064
1
1943
1966
annotation2382068
1
BBa_K1486000
range2382068
1
1967
2668
annotation2382059
1
BBa_B0034
range2382059
1
1211
1222
annotation2382063
1
BBa_I746916
range2382063
1
1223
1942
BBa_K1321346
1
BBa_K1321346
CBDclos fused to sfGFP in RFC 25
Chris N Micklem
2014-10-07T11:00:00Z
2015-06-17T12:30:31Z
false
false
_1696_
4206
20830
9
In stock
false
asdf
asdf
asdf
false
component2415121
1
BBa_K1321002
component2415125
1
BBa_K1321337
component2415123
1
BBa_B0105
annotation2415123
1
BBa_B0105
range2415123
1
301
306
annotation2415121
1
BBa_K1321002
range2415121
1
1
300
annotation2415125
1
BBa_K1321337
range2415125
1
307
1017
BBa_K1399019
1
BBa_K1399019
Ptet-GFP(AAV)
Anna Stikane
2014-09-18T11:00:00Z
2015-05-08T01:10:16Z
false
false
_1777_
0
22477
9
In stock
false
Tetracycline/aTc inducible promoter with GFP reporter tagged with AAV-ssrA degradation tag followed by terminator.
The tagged GFP is actively degraded within cell, thus provides better temporal resolution of green fluorescence and promoter activity.
Part was assembled using BrickClip assembly (BBF RFC104) using other biobrick parts as templates. BrickClip assembly is a special case of more general Paperclip assembly method.[1]
other biobricks
false
component2384238
1
BBa_B0015
component2384231
1
BBa_K1399005
component2384225
1
BBa_B0034
component2384219
1
BBa_R0040
annotation2384231
1
BBa_K1399005
range2384231
1
81
833
annotation2384219
1
BBa_R0040
range2384219
1
1
54
annotation2384225
1
BBa_B0034
range2384225
1
63
74
annotation2384238
1
BBa_B0015
range2384238
1
842
970
BBa_K1680017
1
BBa_K1680017
Cre-Dronpa Fusion
Nicolai von K??gelgen
2015-09-16T11:00:00Z
2016-01-26T10:15:17Z
false
false
_2098_
4206
26366
9
false
Protein coding region for a fusion construct of Cre Recombinase and 145N Dronpa with intermediate 6 amino acid linker. Oligomerisation of Dronpa should stop Cre from accessing DNA in the nucleus thereby inhibiting it until light deactivation of dronpa.
Assembled using 3A assembly.
gBlocks designed by Team Tuebingen.
false
annotation2465979
1
BBa_K1680006
range2465979
1
1064
1732
annotation2465976
1
BBa_K1680000
range2465976
1
1
6
annotation2465978
1
BBa_K1680000
range2465978
1
1050
1055
annotation2465977
1
BBa_K1680007
range2465977
1
13
1041
BBa_K1486034
1
BBa_K1486034
Yeast optimized superfolder GFP C-terminus (215-238)
EPFL iGem team 2014
2014-09-26T11:00:00Z
2015-05-08T01:10:42Z
false
false
_1866_
0
21042
9
Not in stock
false
To edit
To edit
To edit
false
annotation2417784
1
Yeast optimized sfGFP C-terminus
range2417784
1
1
75
BBa_K1321348
1
BBa_K1321348
sfGFP fused to dCBD with linker in RFC 25
Chris N Micklem
2014-10-07T11:00:00Z
2015-06-17T12:30:41Z
false
false
_1696_
4206
20830
9
In stock
false
asdf
asdf
asdf
false
component2415138
1
BBa_B0105
component2415136
1
BBa_K1321337
component2415140
1
BBa_K1321340
annotation2415140
1
BBa_K1321340
range2415140
1
718
1083
annotation2415136
1
BBa_K1321337
range2415136
1
1
711
annotation2415138
1
BBa_B0105
range2415138
1
712
717
BBa_K1673410
1
BBa_K1673410
GFP (Mutation Optimized)
Jarrod Shilts
2015-09-17T11:00:00Z
2016-01-21T02:30:04Z
false
false
_2091_
4206
19415
9
false
GFP, based on E0040, with overall mutation minimized
Sequence produced by the Vanderbilt iGEM 2015 mutation optimization software
DNA Synthesis
false
BBa_K1399020
1
BBa_K1399020
Ptet-GFP(LVA)
Anna Stikane
2014-09-18T11:00:00Z
2015-05-08T01:10:16Z
false
false
_1777_
0
22477
9
It's complicated
false
Tetracycline/aTc inducible promoter with GFP reporter tagged with LVA-ssrA degradation tag followed by terminator.
The tagged GFP is actively degraded within cell, thus provides better temporal resolution of green fluorescence and promoter activity.
Part was assembled using BrickClip assembly (BBF RFC104) using other biobrick parts as templates. BrickClip assembly is a special case of more general Paperclip assembly method.[1]
other biobricks
false
component2384251
1
BBa_K1399004
component2384239
1
BBa_R0040
component2384258
1
BBa_B0015
component2384245
1
BBa_B0034
annotation2384258
1
BBa_B0015
range2384258
1
842
970
annotation2384239
1
BBa_R0040
range2384239
1
1
54
annotation2384245
1
BBa_B0034
range2384245
1
63
74
annotation2384251
1
BBa_K1399004
range2384251
1
81
833
BBa_E0040
1
GFP
green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212
jcbraff
2004-09-29T11:00:00Z
2016-01-26T02:09:38Z
false
true
_11_1_
4206
61
7
Released HQ 2013
In stock
false
GFP (mut3b) [note that this part does not have a barcode]
true
annotation1934520
1
GFP protein
range1934520
1
1
720
BBa_K1468000
1
BBa_K1468000
pJ23104 + gene encoding ZsGreen
Pedro Luis Dorado Morales
2014-10-02T11:00:00Z
2015-05-08T01:10:38Z
false
false
_1847_
0
11756
9
It's complicated
true
-
-
-
false
annotation2418064
1
STOP
range2418064
1
725
725
annotation2418060
1
BBa_E0040
range2418060
1
34
725
annotation2418058
1
BBa_J23104
range2418058
1
1
33
annotation2418062
1
ZsGreeen
range2418062
1
34
725
annotation2418810
1
BBa_K1468000
range2418810
1
1
725
annotation2418061
1
START
range2418061
1
34
36
annotation2418030
1
J23104p
range2418030
1
1
33
annotation2418059
1
ZsGreen protein
range2418059
1
34
725
BBa_K863121
1
GFP_His
green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (His-tag)
Moritz M??ller
2012-09-19T11:00:00Z
2016-01-25T01:33:16Z
false
false
_1123_
4206
13080
9
Not in stock
false
Untagged version of gfp from Repressilator reporter. See the [http://partsregistry.org/wiki/index.php?title=Part:BBa_E0040 untagged version] for source information.
Added Freiburg-restriction-sites for in frame assembly.
[http://partsregistry.org/Part:BBa_I13522 BBa_I13522]
false
annotation2187748
1
ATG
range2187748
1
1
3
annotation2187765
1
BBa_E0040
range2187765
1
10
723
annotation2187751
1
TAA
range2187751
1
748
750
annotation2187747
1
NgoMIV
range2187747
1
4
9
annotation2187756
1
AgeI
range2187756
1
742
747
annotation2187759
1
His-Tag
range2187759
1
724
741
BBa_K082003
1
BBa_K082003
GFP(+LVA)
Yanting Xue , He Shen
2008-10-27T12:00:00Z
2016-01-25T04:43:13Z
false
false
_228_
4206
1560
9
Released HQ 2013
In stock
false
noen
none
none
false
annotation1993327
1
LVA
range1993327
1
721
753
annotation1992959
1
GFP
range1992959
1
1
720
BBa_K1321342
1
BBa_K1321342
sfGFP fused to CBDcex in RFC 25
Chris N Micklem
2014-10-07T11:00:00Z
2015-06-17T12:26:42Z
false
false
_1696_
4206
20830
9
In stock
false
sadf
asdf
asdf
false
component2415361
1
BBa_B0105
component2415362
1
BBa_K1321003
component2415359
1
BBa_K1321337
annotation2415361
1
BBa_B0105
range2415361
1
712
717
annotation2415359
1
BBa_K1321337
range2415359
1
1
711
annotation2415362
1
BBa_K1321003
range2415362
1
718
1044
BBa_K2023016
1
BBa_K2023016
GFP coding device with Pu
C??lia Chenebault
2016-10-13T11:00:00Z
2016-10-20T04:05:09Z
false
false
_2490_
30356
30230
9
false
GFP coding device with Pu
blabla
Pu promoter and RBS from GLuc coding device with Pu (BBa_K2023003) and GFP reporter for RHS of library test constructs (BBa_I13401)
false
component2518994
1
BBa_I723020
component2519005
1
BBa_B0015
component2518998
1
BBa_E0040
component2518996
1
BBa_B0034
annotation2518996
1
BBa_B0034
range2518996
1
329
340
annotation2518998
1
BBa_E0040
range2518998
1
347
1066
annotation2518994
1
BBa_I723020
range2518994
1
1
320
annotation2519005
1
BBa_B0015
range2519005
1
1075
1203
BBa_K1980008
1
BBa_K1980008
pCopA CueR sfGFP/ feedback pCopA sfGFP
Sam Garforth
2016-10-10T11:00:00Z
2016-10-24T07:34:54Z
false
false
_2447_
29607
29607
9
false
pCopA CueR sfGFP/ feedback pCopA sfGFP
codon op
E. coli
false
annotation2528249
1
pCopA
range2528249
1
1
51
annotation2528252
1
sfGFP
range2528252
1
515
1228
annotation2528253
1
RBS
range2528253
1
499
504
annotation2528251
1
CueR
range2528251
1
76
480
annotation2528254
1
Hexahistidine
range2528254
1
1229
1246
annotation2528250
1
RBS
range2528250
1
58
69
annotation2528256
1
Stop
range2528256
1
481
486
annotation2528255
1
Stop
range2528255
1
1247
1252
BBa_K863120
1
GFP_Freibu
green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (Freiburg-Standard)
Moritz M??ller
2012-09-19T11:00:00Z
2016-01-25T01:32:59Z
false
false
_1123_
4206
13080
9
Not in stock
false
Untagged version of gfp from Repressilator reporter. See the [http://partsregistry.org/wiki/index.php?title=Part:BBa_E0040 untagged version] for source information.
Added Freiburg-restriction-sites for in frame assembly.
[http://partsregistry.org/Part:BBa_I13522 BBa_I13522]
false
annotation2187770
1
NgoMIV
range2187770
1
4
11
annotation2187771
1
TAA
range2187771
1
727
729
annotation2187772
1
AgeI
range2187772
1
721
726
annotation2187773
1
BBa_E0040
range2187773
1
12
720
annotation2187769
1
ATG
range2187769
1
1
3
BBa_K1321341
1
BBa_K1321341
sfGFP fused to CBDclos in RFC 25
Chris N Micklem
2014-10-07T11:00:00Z
2015-06-17T12:27:02Z
false
false
_1696_
4206
20830
9
In stock
false
asdfads
asdfd
asdfad
false
component2415088
1
BBa_K1321337
component2415090
1
BBa_B0105
component2415091
1
BBa_K1321002
annotation2415088
1
BBa_K1321337
range2415088
1
1
711
annotation2415091
1
BBa_K1321002
range2415091
1
718
1017
annotation2415090
1
BBa_B0105
range2415090
1
712
717
BBa_K1399018
1
BBa_K1399018
Plac-GFP(DAS)
Anna Stikane
2014-09-18T11:00:00Z
2015-05-08T01:10:16Z
false
false
_1777_
0
22477
9
In stock
true
Lactose/IPTG inducible promoter with GFP reporter tagged with DAS-ssrA degradation tag followed by terminator.
The tagged GFP is actively degraded within cell, thus provides better temporal resolution of green fluorescence and promoter activity. Presence of SspB is crucial for effective protein degradation.
Part was assembled using BrickClip assembly (BBF RFC104) using other biobrick parts as templates. BrickClip assembly is a special case of more general Paperclip assembly method.[1]
other biobricks
false
component2384211
1
BBa_K1399008
component2384197
1
BBa_R0010
component2384205
1
BBa_B0034
component2384218
1
BBa_B0015
annotation2384218
1
BBa_B0015
range2384218
1
988
1116
annotation2384211
1
BBa_K1399008
range2384211
1
227
979
annotation2384205
1
BBa_B0034
range2384205
1
209
220
annotation2384197
1
BBa_R0010
range2384197
1
1
200
BBa_K125500
1
BBa_K125500
GFP fusion brick
Grace Kwan
2008-07-24T11:00:00Z
2016-01-25T02:34:56Z
false
false
_179_
4206
3229
9
In stock
false
The first two nucleotides of the GFP start codon were removed to create this fusion brick.
The GFP fusion brick was derived from the GFP BioBrick, [http://partsregistry.org/Part:BBa_E0040 BBa_E0040].
false
annotation1968260
1
GFP fusion brick
range1968260
1
1
718
annotation1969908
1
deletion of TG
range1969908
1
1
1
BBa_K1700000
1
BBa_K1700000
Use toehold to regulate GFP
Meixi Liu
2015-09-16T11:00:00Z
2015-09-17T09:57:13Z
false
false
_2118_
28512
28512
9
false
The structure of toehold switch is similar to hairpin, except it has a loop at the top as ???toehold???. Toehold switch functions as riboregulator through linear-linear interaction between RNAs. When target RNA appears, it will bind one of the toehold switch stems and open the loop, exposing the RBS.
Toehold switch systems are composed of two RNA strands referred to as the switch and trigger. The switch RNA contains the coding sequence of the gene being regulated. Upstream of this coding sequence is a hairpin-based processing module containing both a strong RBS and a start codon that is followed by a common 21 nt linker sequence coding for low-molecular-weight amino acids added to the N terminus of the gene of interest. A single-stranded toehold sequence at the 50 end of the hairpin module provides the initial binding site for the trigger RNA strand. This trigger molecule contains an extended single-stranded region that completes a branch migration process with the hairpin to expose the RBS and start codon, thereby initiating translation of the gene of interest.
The trigger RNA of our toehold is miRNA-144.When adding it to the system, GFP can be expressed.
Toehold can be open by trigger RNA, thus letting GFP express.
We synthesis the part.
false
annotation2467629
1
ATG start codon
range2467629
1
156
158
annotation2467633
1
TAA stop codon
range2467633
1
818
820
annotation2467606
1
toehold
range2467606
1
1
100
annotation2467632
1
GFP coding sequence
range2467632
1
101
820
BBa_K1709002
1
CheZ-GFP
CheZ-GFP
Astrid Deryckere
2015-09-09T11:00:00Z
2015-09-18T09:28:45Z
false
false
_2129_
25389
25389
9
true
Upon recognition of a small molecule (e.g. nutrients or toxins), chemoreceptors transduce a signal through a set of Che proteins. This group of methylesterases and phosphatases regulates the rotation of the flagella. At the end of the chain of Che proteins is CheY. In phosphorylated state, CheY binds the flagella and causes the cells to tumble. The phosphatase CheZ dephosphorylates CheY, inducing dissociation of CheY from the flagella enabling the cells to swim. Cells lacking the CheZ protein are unable to swim and will tumble excessively and incessantly.
1 bp in BBa_K629003 was adapted to create an original restriciton site in the plasmid.
CheZ was fused to GFP (BBa_K082003) and its LVA tag was codon-optimized to avoid introducing artificial DNA repetitions.
DNA sequences originate from the E. Coli genome and previous biobrick sequences.
false
annotation2448532
1
CheZ Coding Region
range2448532
1
1
642
annotation2448533
1
GFP Coding Region
range2448533
1
646
1365
annotation2448531
1
Ochre Stop Codon
range2448531
1
1399
1401
annotation2448534
1
LVA-tag
range2448534
1
1366
1401
annotation2448530
1
ATG Start Codon
range2448530
1
1
3
BBa_K411207
1
GFPLVA
Green Fluorescent Protein with LVA tag (GFPLVA)
KO KUEI YUEH
2010-10-21T11:00:00Z
2016-01-28T12:22:52Z
false
true
_526_
4206
6665
9
In stock
false
GFP with LVA tag can be degrage by SspB
GFP with LVA-tag can be degrade by speedy degrager and has a short half-life
Biobrick E0040
false
annotation2092218
1
GFP
range2092218
1
1
714
annotation2101796
1
stop
range2101796
1
748
753
annotation2092217
1
LVA tag
range2092217
1
715
747
BBa_K294055
1
BBa_K294055
GFP RFP Hybrid
Ryoji Sekine
2009-06-26T11:00:00Z
2016-01-25T02:34:10Z
false
false
_397_
4206
5313
9
Released HQ 2013
In stock
false
This is a test. It may not bright.
E0040
ATGCATATATATAATTT
false
annotation2006753
1
Hoge
range2006753
1
200
500
annotation2006752
1
hogehoge
range2006752
1
1
100
BBa_K1486039
1
BBa_K1486039
sfGFPC
EPFL iGem team 2014
2014-09-26T11:00:00Z
2015-05-08T01:10:42Z
false
false
_1866_
0
21042
9
Not in stock
false
To edit
To edit
To edit
false
BBa_K1824100
1
BBa_K1824100
eGFP-terminater
Xinhao Wang
2015-09-12T11:00:00Z
2015-09-13T02:49:04Z
false
false
_2250_
26000
26000
9
false
Composed of eGFP-rrnB terminator-T7 terminater, canbe used in further assembly.
Mind the 5'UTR if in a complex construct.
synthetic construct
false
component2453149
1
BBa_K1824889
component2453156
1
BBa_B0015
component2453148
1
BBa_K1094400
annotation2453149
1
BBa_K1824889
range2453149
1
724
731
annotation2453148
1
BBa_K1094400
range2453148
1
1
723
annotation2453156
1
BBa_B0015
range2453156
1
732
860
BBa_K1399021
1
BBa_K1399021
Ptet-GFP(LAA)
Anna Stikane
2014-09-18T11:00:00Z
2015-05-08T01:10:16Z
false
false
_1777_
0
22477
9
It's complicated
false
Tetracycline/aTc inducible promoter with GFP reporter tagged with LAA-ssrA degradation tag followed by terminator.
The tagged GFP is actively degraded within cell, thus provides better temporal resolution of green fluorescence and promoter activity.
Part was assembled using BrickClip assembly (BBF RFC104) using other biobrick parts as templates. BrickClip assembly is a special case of more general Paperclip assembly method.[1]
other parts
false
component2384259
1
BBa_R0040
component2384278
1
BBa_B0015
component2384265
1
BBa_B0034
component2384271
1
BBa_K1399006
annotation2384278
1
BBa_B0015
range2384278
1
842
970
annotation2384259
1
BBa_R0040
range2384259
1
1
54
annotation2384271
1
BBa_K1399006
range2384271
1
81
833
annotation2384265
1
BBa_B0034
range2384265
1
63
74
BBa_K1486040
1
BBa_K1486040
sfGFPN + CpxR
EPFL iGem team 2014
2014-09-26T11:00:00Z
2015-05-08T01:10:42Z
false
false
_1866_
0
21042
9
Not in stock
false
To edit
To edit
To edit
false
component2387188
1
BBa_K1486038
component2387187
1
BBa_B0034
component2387185
1
BBa_I0500
component2387193
1
BBa_K1486000
component2387189
1
BBa_K1486037
annotation2387185
1
BBa_I0500
range2387185
1
1
1210
annotation2387187
1
BBa_B0034
range2387187
1
1211
1222
annotation2387188
1
BBa_K1486038
range2387188
1
1223
1864
annotation2387189
1
BBa_K1486037
range2387189
1
1865
1903
annotation2387193
1
BBa_K1486000
range2387193
1
1904
2605
BBa_K1602021
1
HisTag-GFP
Green fluorescent protein (GFP) with N-terminal His-Tag
Jasmin Weber, Michelle Zoeller, Marcus Geissler, Stefan Bohn, Simon Linder, Sebastian Barthel
2015-09-15T11:00:00Z
2016-01-25T01:10:25Z
false
false
_2019_
0
4206
22022
9
It's complicated
false
false
BBa_K1468002
1
BBa_K1468002
pJ23110 + gene encoding ZsGreen
Pedro Luis Dorado Morales
2014-10-02T11:00:00Z
2015-05-08T01:10:38Z
false
false
_1847_
0
11756
9
It's complicated
false
-
-
-
false
BBa_K1930006
1
sfGFP(Sp)
sfGFP(Sp)
Barbora Waclawikova
2016-10-15T11:00:00Z
2016-10-16T05:16:06Z
false
false
_2397_
29748
29748
9
false
This part is a reporter sfGFP(Sp) originally optimized for <em>Streptococcus pneumoniae</em>.
sfGFP originally optimized for streptococcus pneumonia because it could be shown that the signal in Bacillus is even brighter than the one from sfGFP(Bs).
Plasmid pNW
false
annotation2513522
1
sfGFP(Sp)
range2513522
1
1
722
BBa_K1486038
1
BBa_K1486038
sfGFPN
EPFL iGem team 2014
2014-09-26T11:00:00Z
2015-05-08T01:10:42Z
false
false
_1866_
0
21042
9
Not in stock
false
To edit
To edit
To edit
false
BBa_K648013
1
BBa_K648013
GFP with Standard 25 Prefix/Suffix
Jim Rose
2011-07-03T11:00:00Z
2016-01-25T02:32:21Z
false
false
_825_
4206
9871
9
In stock
false
This is the standard GFP protein reporter (E0040) cloned with the prefix/suffix required for standard 25 assembly. It can easily be used to create fusion proteins through this method.
This part contains the AgeI and NgoMIV sites as part of it's prefix/suffix that allows it to be used in standard 25 assembly.
It was synthesized through PCR oligo synthesis methods using the following primers:
Forward (56.88 degrees)
TATTGAATTCGCGGCCGCTTCTAGATGGCCGGCCGTAAAGGAGAAGAACTTTTC
Reverse (56.95 degrees)
AATACTGCAGCGGCCGCTACTAGTATTAACCGGTCTTGTCGTCATCATCTTTATAAT
This part is similar to the part BBa_E0040 commonly used as a reporter.
false
annotation2122827
1
misc
range2122827
1
1
735
BBa_K1909013
1
BBa_K1909013
Omp Promoter / GFP
Daniel Wedemeyer
2016-10-13T11:00:00Z
2016-10-14T12:55:41Z
false
false
_2375_
29525
29525
9
false
Coming Soon
Coming Soon
Composite Part
false
component2508847
1
BBa_E0240
component2508836
1
BBa_R0082
annotation2508836
1
BBa_R0082
range2508836
1
1
108
annotation2508847
1
BBa_E0240
range2508847
1
117
992
BBa_K106671
1
BBa_K106671
GFP, Aar1 AD part
Jacinto Chen
2008-10-28T12:00:00Z
2016-01-26T09:50:57Z
false
false
_226_
4206
3418
9
It's complicated
true
This is the coding sequence of a yeast optimized GFP with BD ends for AarI cloning.
As an AD part, there is no ATG, and the stop codon is included. For more information on AarI cloning, please refer to the UCSF 2008 igem page.
PCR amplified from lab plasmid stocks.
true
annotation1993686
1
BBa_K106671
range1993686
1
1
714
BBa_K1980005
1
BBa_K1980005
pCopA sfGFP
Sam Garforth
2016-10-10T11:00:00Z
2016-10-23T04:46:35Z
false
false
_2447_
29607
29607
9
It's complicated
false
This is the copper sensitive promoter pCopA (controlled by CueR) in front of an RBS and a sfGFP reporter
Codon optised to E. coli.
E. coli genome.
false
annotation2528239
1
pCopA
range2528239
1
1
51
annotation2528243
1
Stop
range2528243
1
805
810
annotation2528240
1
RBS
range2528240
1
57
62
annotation2528242
1
Hexahis
range2528242
1
787
804
annotation2528241
1
sfGFP
range2528241
1
73
786
annotation2528664
1
CueR binding
range2528664
1
19
37
BBa_K1497020
1
BBa_K1497020
Naringenin sensor (FdeR) with GFP as reporter
Sascha Hein, Niklas Hummel, Sebastian Barthel, Thomas Dohmen
2014-10-06T11:00:00Z
2015-05-08T01:10:45Z
false
false
_1877_
0
12466
9
It's complicated
false
We modified the naringenin biosensor from Siedler et al. by elimination of one EcoRI restriction site and by changing the native ribosomal binding site into the strong RBS Bba_B0034. Based on this reporter protein and 3 different fluorescence proteins, we designed 3 new biosensors for in vivo detection and determination of naringenin. The natural source of FdeR is Herbaspirillum seropedicae.
You can use our reporter for measuring naringenin concentrations in your samples. Depending on which fluorophor you want to detect, you can use one of three biosensors:
GFP fluorescence K1497020
mKate (DsRed variant) fluorescence K1497021
CFP fluorescence K1497022
You can create your own naringenin sensor or your own naringenin dependent gene expression device as well. For these reasons use FdeR Biobrick K1497019 and clone your parts of interest (without RBS!) behind the device.
d
d
false
component2398949
1
BBa_E0040
component2398947
1
BBa_K1497019
annotation2398949
1
BBa_E0040
range2398949
1
1221
1940
annotation2398947
1
BBa_K1497019
range2398947
1
1
1214
BBa_K1680019
1
BBa_K1680019
Cre-dronpa fusion
Nicolai von Kuegelgen
2015-09-16T11:00:00Z
2016-01-26T10:15:55Z
false
false
_2098_
4206
26366
9
false
Protein coding region for a fusion construct of Cre Recombinase and 145N Dronpa with 12 amino acid linker. Oligomerisation of Dronpa should stop Cre from accessing DNA in the nucleus thereby inhibiting it until light deactivation of dronpa.
3A assembly using RFC25
gBlocks designed by Team Tuebingen
false
annotation2477417
1
K1680002
range2477417
1
1060
1095
annotation2477415
1
K1680002
range2477415
1
1
30
annotation2477416
1
K1680007
range2477416
1
31
1059
annotation2477418
1
K1680006
range2477418
1
1096
1764
BBa_K1620005
1
pUSPA::gfp
GFP device responsive to environmental stresses
Celio Dias Santos Jr
2015-09-02T11:00:00Z
2015-09-13T03:59:02Z
false
false
_2037_
23597
23597
9
false
This device takes environmental stresses as input a transcriptional signal (PoPS) and produce as output the fluorescent protein GFP.
This part was assembled using 3A assembly method.
This part is an assembly of promoter uspA (BBa_K1620000) and GFP generator device (BBa_E0840).
false
component2443245
1
BBa_E0840
component2443234
1
BBa_K1620000
annotation2443245
1
BBa_E0840
range2443245
1
55
932
annotation2443234
1
BBa_K1620000
range2443234
1
1
46
BBa_K1680006
1
BBa_K1680006
Fluorescent protein dronpa
Katharina Sporbeck
2015-09-16T11:00:00Z
2016-01-26T10:15:05Z
false
false
_2098_
4206
26460
9
false
Protein coding sequence for a mutant (K145N) of the fluorescent protein Dronpa, codon optimized for Yeast. The mutation leads to photoswitchable monomerisation/multimerisation behaviour (as described by Zhou et al. 2012).
Part is shipped in RFC25.
gBlock designed by Team Tuebingen
false
annotation2475196
1
Dronpa
range2475196
1
1
669
BBa_K1602024
1
GFP-Si4Tag
HisTag-GFP_Si4-Tag fusion protein
Jasmin Weber, Michelle Zoeller, Marcus Geissler, Stefan Bohn, Simon Linder, Sebastian Barthel
2015-09-15T11:00:00Z
2016-01-25T01:11:28Z
false
false
_2019_
4206
22022
9
false
false
BBa_K1321349
1
BBa_K1321349
CBDcex fused to sfGFP in RFC 25
Chris N Micklem
2014-10-07T11:00:00Z
2015-06-17T12:30:55Z
false
false
_1696_
4206
20830
9
In stock
false
asdf
asdf
asdf
false
component2415151
1
BBa_B0105
component2415149
1
BBa_K1321003
component2415153
1
BBa_K1321337
annotation2415153
1
BBa_K1321337
range2415153
1
334
1044
annotation2415149
1
BBa_K1321003
range2415149
1
1
327
annotation2415151
1
BBa_B0105
range2415151
1
328
333
BBa_K1650038
1
BBa_K1650038
GFP-His
Alexandra Richter
2015-09-17T11:00:00Z
2016-01-21T02:24:59Z
false
false
_2067_
4206
27246
9
false
test
test
test
false
annotation2474934
1
BBa_K1650038
range2474934
1
1
1159
BBa_K180001
1
BBa_K180001
Green fluorescent protein (+LVA)
Richard Wilson, Stephanie Chambers
2009-10-11T11:00:00Z
2016-01-25T01:37:25Z
false
false
_278_
4206
4082
9
Not in stock
true
Based on the part E0040, but with an LVA degradation tail attached.
LVA degradation tail and prefix/suffix addition by sequential PCR amplifications with specially designed primers.
Based upon GFP from E0040, with LVA degradation tail attached.
false
annotation2034727
1
LVA
range2034727
1
721
754
annotation2034728
1
K180001
range2034728
1
1
754
annotation2034726
1
GFPmut3b
range2034726
1
1
720
BBa_K1742003
1
BBa_K1742003
Dronpa 145K
Iv??n Casas Rodrigo
2015-09-03T11:00:00Z
2016-01-25T11:27:33Z
false
false
_2164_
4206
26598
9
false
From Pectinidae sp.
It is a CDS of green fluorescent protein Dronpa.
It is codon optimized for plants.
Pectinidae sp. sequence found in the following article Lummer, M., Humpert, F., Steuwe, C., Caesar, K., Sch??ttpelz, M., Sauer, M. and Staiger, D. (2011), Reversible Photoswitchable DRONPA-s Monitors Nucleocytoplasmic Transport of an RNA-Binding Protein in Transgenic Plants. Traffic, 12: 693???702. doi: 10.1111/j.1600-0854.2011.01180.x
false
BBa_K1789004
1
BBa_K1789004
GFP2
Xinyuan Qiu
2015-09-13T11:00:00Z
2016-01-25T01:04:48Z
false
false
_2214_
4206
21134
9
false
This part is the Carboxyl Half of GFP
we REMOVED the extra base from part BBa_I715020(easier to fuse with other proteins) when designing PCR primers to make the protein fusion easier.
This part is gained from part BBa_I715020.
false
annotation2454067
1
GFP2
range2454067
1
1
249
BBa_K1486023
1
BBa_K1486023
Yeast optimized superfolder GFP
EPFL iGem team 2014
2014-09-25T11:00:00Z
2015-05-08T01:10:42Z
false
false
_1866_
0
21042
9
Not in stock
false
To edit
To edit
To edit
false
annotation2418775
1
Yo sfGFP
range2418775
1
1
717
BBa_K1467204
1
BBa_K1467204
GoldenGate compatible Green Flourescent Protein (GFP)
Cara Deal
2014-09-23T11:00:00Z
2015-06-01T07:05:42Z
false
false
_1846_
4206
21550
9
In stock
false
A variation of the part BBa_E0040 that has been made compatible for Golden Gate by removal of internal BsaI and BpiI recognition sequences.
Fluoresces under UV light when the gene is expressed.
Domestication for GoldenGate by removal of internal BsaI and BpiI recognition sequences.
Aequorea victoria
false
BBa_K1620006
1
J23101::gf
Constitutive GFP generator device
Celio Dias Santos Jr
2015-09-02T11:00:00Z
2015-09-18T03:02:02Z
false
false
_2037_
23597
23597
9
It's complicated
false
It generates permanently and a low rate GFP reporter. It is usefull for cell marker and promoter strength tests.
Assembly method used was 3A assembly.
This part is assembled from parts BBa_J23101 and GFP generator (BBa_E0840).
false
component2443257
1
BBa_E0840
component2443246
1
BBa_J23101
annotation2443246
1
BBa_J23101
range2443246
1
1
35
annotation2443257
1
BBa_E0840
range2443257
1
44
921
BBa_K1899003
1
BBa_K1899003
Superfolder GFP
iGEM2016_HKUST
2016-08-27T11:00:00Z
2016-10-15T04:03:09Z
false
false
_2365_
26069
26069
9
false
Variant of GFP that folds robustly even when fused to poorly folded proteins.
Waldo and other research groups has reported the engineering of a superfolder GFP (sfGFP) that showed increased resistance to denaturation, improved folding kinetics, and increased resistance to aggregation during refolding (Pedelacq et al., 2006; Andrews et al., 2007; Fisher and DeLisa, 2008). sfGFP has proven to be very useful as a scaffold for improved protein detection and tagging both in vivo and in vitro using self-assembled sfGFP fragments (Cabantous et al., 2005b; Cabantous and Waldo, 2006). Furthermore, sfGFP fusions are more soluble than conventional GFP fusions (Wu et al., 2009).
nil
Waldo lab
false
annotation2481805
1
BBa_J61048
range2481805
1
742
854
annotation2481806
1
scar
range2481806
1
734
741
annotation2481804
1
scar
range2481804
1
13
19
annotation2481802
1
BBa_B0032
range2481802
1
1
12
annotation2481803
1
sfGFP
range2481803
1
20
733
BBa_K1709003
1
BBa_K1709003
CheZ-GFP
Astrid Deryckere
2015-09-15T11:00:00Z
2015-09-26T08:47:17Z
false
false
_2129_
25389
25389
9
true
Upon recognition of a small molecule (e.g. nutrients or toxins), chemoreceptors transduce a signal through a set of Che proteins. This group of methylesterases and phosphatases regulates the rotation of the flagella. At the end of the chain of Che proteins is CheY. In phosphorylated state, CheY binds the flagella and causes the cells to tumble. The phosphatase CheZ dephosphorylates CheY, inducing dissociation of CheY from the flagella enabling the cells to swim. Cells lacking the CheZ protein are unable to swim and will tumble excessively and incessantly.
1 bp in BBa_K629003 was adapted to create an original restriciton site in the plasmid. CheZ was fused to GFP (BBa_K082003) and its LVA tag was codon-optimized to avoid introducing artificial DNA repetitions.
DNA sequences originate from the E. Coli genome and previous biobrick sequences. The ribosome binding site was chosen from the Anderson RBS family.
false
component2472552
1
BBa_K1709002
component2472546
1
BBa_J61101
annotation2472552
1
BBa_K1709002
range2472552
1
19
1419
annotation2472546
1
BBa_J61101
range2472546
1
1
12
BBa_K1486041
1
BBa_K1486041
sfGFPC + CpxR
Ione Pla Puigsubir??
2014-09-26T11:00:00Z
2015-05-08T01:10:42Z
false
false
_1866_
0
21042
9
Not in stock
false
To edit
To edit
To edit
false
component2387213
1
BBa_K1486037
component2387209
1
BBa_I0500
component2387212
1
BBa_K1486039
component2387217
1
BBa_K1486000
component2387211
1
BBa_B0034
annotation2387212
1
BBa_K1486039
range2387212
1
1223
1294
annotation2387211
1
BBa_B0034
range2387211
1
1211
1222
annotation2387213
1
BBa_K1486037
range2387213
1
1295
1333
annotation2387209
1
BBa_I0500
range2387209
1
1
1210
annotation2387217
1
BBa_K1486000
range2387217
1
1334
2035
BBa_K1680024
1
BBa_K1680024
Dronpa caged Cre with NLS
Nicolai von Kuegelgen
2015-09-16T11:00:00Z
2016-01-26T10:22:01Z
false
false
_2098_
4206
26366
9
false
Protein coding region for a fusion protein of Cre recombinase (BBa_K1680007) that is C- & N-terminal flanked with 145N Dronpa (BBa_K1680006) using a 15 AA linker as spacer (BBa_K1680003). The construct has a N-terminal NLS sequence (BBa_K1680004). In the expressed construct the two dronpa domains should form multimerize leading to an inactive Cre recombinase that is reversibly activatable through dronpa illumination (see Zhou et al 2012).
3A assembly with RFC25
gBlock design by Team Tuebingen
false
annotation2477394
1
K1680006
range2477394
1
1818
2487
annotation2477393
1
K1680006
range2477393
1
31
699
annotation2477392
1
K1680007
range2477392
1
745
1773
annotation2477390
1
K1680004
range2477390
1
1
24
annotation2477396
1
K1680003
range2477396
1
1774
1817
annotation2477395
1
K1680003
range2477395
1
700
744
BBa_K1680018
1
BBa_K1680018
Cre-Dronpa fusion
Nicolai von K??gelgen
2015-09-16T11:00:00Z
2016-01-26T10:15:36Z
false
false
_2098_
4206
26366
9
false
Protein coding region for a fusion construct of Cre Recombinase and 145N Dronpa with 9 amino acid linker. Oligomerisation of Dronpa should stop Cre from accessing DNA in the nucleus thereby inhibiting it until light deactivation of dronpa.
Assembled using 3A assembly and RFC25.
gBlocks designed by Team Tuebingen
false
annotation2477358
1
K1680001
range2477358
1
1051
1077
annotation2477354
1
K1680006
range2477354
1
1078
1746
annotation2477357
1
K1680001
range2477357
1
1
21
annotation2477355
1
K1680007
range2477355
1
22
1050
BBa_K1680023
1
BBa_K1680023
Dronpa caged Cre with NLS
Nicolai von Kuegelgen
2015-09-16T11:00:00Z
2016-01-26T10:42:40Z
false
false
_2098_
4206
26366
9
false
Protein coding region for a fusion protein of Cre recombinase (BBa_K1680007) that is C- & N-terminal flanked with 145N Dronpa (BBa_K1680006) using a 12 AA linker as spacer (BBa_K1680002). The construct has a N-terminal NLS sequence (BBa_K1680004). In the expressed construct the two dronpa domains should form multimerize leading to an inactive Cre recombinase that is reversibly activatable through dronpa illumination (see Zhou et al 2012).
3A assembly using RFC25
gBlock design by Team Tuebingen
false
annotation2477382
1
K1680007
range2477382
1
736
1764
annotation2477383
1
K1680002
range2477383
1
700
735
annotation2477380
1
K1680006
range2477380
1
31
699
annotation2477381
1
K1680006
range2477381
1
1801
2469
annotation2477379
1
K1680004
range2477379
1
1
24
annotation2477384
1
K1680002
range2477384
1
1765
1800
BBa_K1742002
1
BBa_K1742002
Dronpa 145N
Iv??n Casas Rodrigo
2015-09-03T11:00:00Z
2016-01-25T11:27:00Z
false
false
_2164_
4206
26598
9
false
A CDS of Dronpa protein with a mutation in the betha-strand 7 in Lys145 for Asn.
It is codon optimized for plants
Pectinidae sp. sequence found in the following article Lummer, M., Humpert, F., Steuwe, C., Caesar, K., Sch??ttpelz, M., Sauer, M. and Staiger, D. (2011), Reversible Photoswitchable DRONPA-s Monitors Nucleocytoplasmic Transport of an RNA-Binding Protein in Transgenic Plants. Traffic, 12: 693???702. doi: 10.1111/j.1600-0854.2011.01180.x
false
BBa_K1399004
1
BBa_K1399004
GFP (mut3b) with LVA-ssrA degradation tag
Anna Stikane
2014-09-18T11:00:00Z
2015-06-01T07:07:29Z
false
false
_1777_
4206
22477
9
In stock
true
GFP (mut3b) (see part BBa_E0040) with added LVA-ssrA degradation tag. The tag increases GFP turn-over rate, thus providing better temporal resolution of green fluorescence. In the same time, maximal fluorescence amplitudes will be lower as newly formed protein is degraded as soon as it is formed.
The tag encodes peptide sequence AANDENYALVA and is recognized by ClpA and ClpX unfoldases and ClpX mediator SspB.[1] ClpA and ClpX then form a proteosome-like complex with ClpP protease and the protein is degraded.[1]
The final three residues of the tag determines the strength of interaction with ClpX and thus the final protein degradation rate.[2] The LVA tag is reported to lead to fast protein degradation, degrading GFP with rate -0.018 per minute.[2] However, be aware that exact protein degradation rate depends on multiple factors: ClpXP and ClpAP protease and SspB mediator concentrations, protein stability, Km of binding to the protease, temperature [3].
References
[1] Flynn, J. M. et al. Overlapping recognition determinants within the ssrA degradation tag allow modulation of proteolysis. Proc. Natl. Acad. Sci. U. S. A. 98, 10584???9 (2001).
[2] Andersen, J. B. et al. New unstable variants of green fluorescent protein for studies of transient gene expression in bacteria. Appl. Environ. Microbiol. 64, 2240???6 (1998).
[3] Purcell, O., Grierson, C. S., Bernardo, M. Di & Savery, N. J. Temperature dependence of ssrA-tag mediated protein degradation. J. Biol. Eng. 6, 10 (2012).
The tag was attached to GFP using PCR and MABEL (mutagenesis with blunt-end ligation), thus avoiding introduction of additonal residues and restriction site. Different parts of the tag are recognized by different proteins, for example, the final 3 residues (LVA in this case) are recognised by ClpX, whereas first 4 residues of the tag are required for efficient SspB binding.[1] Thus modifications of these critical residues alter the efficacy with what different proteases bind to it.
GFP comes from part E1010, the tag sequence was obtained from paper by Andersen et al., (1998).[2]
false
annotation2383913
1
start
range2383913
1
1
3
annotation2383916
1
stop
range2383916
1
748
750
annotation2383914
1
GFP (mut3b)
range2383914
1
4
714
annotation2383917
1
stop
range2383917
1
751
753
annotation2383915
1
LVA
range2383915
1
715
747
BBa_K1399022
1
BBa_K1399022
Ptet-GFP(DAS+2)
Anna Stikane
2014-09-18T11:00:00Z
2015-05-08T01:10:16Z
false
false
_1777_
0
22477
9
In stock
false
Tetracycline/aTc inducible promoter with GFP reporter tagged with NYADAS-ssrA degradation tag followed by terminator.
The tagged GFP is actively degraded within cell, thus provides better temporal resolution of green fluorescence and promoter activity.
Part was assembled using BrickClip assembly (BBF RFC104) using other biobrick parts as templates. BrickClip assembly is a special case of more general Paperclip assembly method.[1]
other parts
false
component2384279
1
BBa_R0040
component2384285
1
BBa_B0034
component2384291
1
BBa_K1399007
component2384298
1
BBa_B0015
annotation2384279
1
BBa_R0040
range2384279
1
1
54
annotation2384285
1
BBa_B0034
range2384285
1
63
74
annotation2384298
1
BBa_B0015
range2384298
1
848
976
annotation2384291
1
BBa_K1399007
range2384291
1
81
839
BBa_J52026
1
BBa_J52026
dnMyD88-linker-GFP
Jelka Pohar
2006-10-14T11:00:00Z
2016-01-26T09:39:38Z
false
false
_80_
4206
855
80
In stock
false
dnMyD88 is a dominant negative form of protein MyD88 (an important protein in signaling cascade through TLR4 receptor). dnMyD88 is linked to GFP (acts as a reporter) through six aminoacid long protein linker.
dnMyD88-linker-GFP was made by using PCR overlap extension technique. This part is cloned in BioBrick vector pSB1AK3. Original sequence has PstI restriction site so we have to introduce a mutation without affect on amino acid sequence.
dnMyD88-
GFP-
more to come
true
annotation1903011
1
dnMyD88-linker-GFP
range1903011
1
1
1155
annotation1903008
1
dnMyD88
range1903008
1
1
417
annotation1903009
1
linker
range1903009
1
418
435
annotation1903010
1
GFP
range1903010
1
436
1155
BBa_K1980001
1
Csp1sfGFP
TAT Copper Storage Protein 1 sfGFP
Sam Garforth
2016-10-10T11:00:00Z
2016-10-12T02:35:29Z
false
false
_2447_
29607
29607
9
false
Copper Storage protein 1 (Csp1) is a tetrameric copper storage protein found in the periplasm of Methylosinus trichosporium OB3b. We investigated whether this part could act as a copper chelator when expressed in E. coli. We modified the protein by adding a TAT signal peptide from the E. coli enzyme CueO in place of the native TAT sequence and added a c terminal sfGFP fluorescence protein with a C terminal his tag in an attempt to direct the protein to the periplasm. The Csp1 and sfGFP are separated by a short hydrophilic, flexible linker.
Codon optimised for E. coli. linker added in order to allow the Csp1 and sfGFP to ideally fold separately. Hexahistidine purification tag on c-terminus to maintain TAT sequence.
The source organism for the main protein sequence is Methylosinus trichosporium OB3b. The TAT sequence originate from E. coli multi copper oxidase enzyme CueO. We ordered it as codon optimised DNA from IDT.
false
annotation2495228
1
Csp1
range2495228
1
85
450
annotation2495227
1
TAT signal peptide
range2495227
1
1
84
annotation2495231
1
Hexahistidine
range2495231
1
1180
1197
annotation2495229
1
linker
range2495229
1
451
468
annotation2495238
1
Double stop
range2495238
1
1198
1203
annotation2495230
1
sfGFP
range2495230
1
469
1179
BBa_K1732020
1
BBa_K1732020
GFP-HIS
Donna Lee
2015-09-15T11:00:00Z
2016-01-25T01:12:20Z
false
false
_2154_
4206
27195
9
false
GFP with a 6X His tag for easier protein purification.
Codon optimized for E.coli.
Unknown
false
annotation2462824
1
GFPCO
range2462824
1
1
713
annotation2462825
1
6x-His
range2462825
1
714
731
BBa_K1680020
1
BBa_K1680020
Cre-dronpa fusion
Nicolai von Kuegelgen
2015-09-16T11:00:00Z
2016-01-26T10:16:19Z
false
false
_2098_
4206
26366
9
false
Protein coding region for a fusion construct of Cre Recombinase and 145N Dronpa with 15 amino acid linker. Oligomerisation of Dronpa should stop Cre from accessing DNA in the nucleus thereby inhibiting it until light deactivation of dronpa.
3A assembly using RFC25
gBlock design by Team Tuebingen
false
annotation2477365
1
K1680003
range2477365
1
1
39
annotation2477363
1
K1680007
range2477363
1
40
1068
annotation2477364
1
K1680006
range2477364
1
1114
1782
annotation2477366
1
K1680003
range2477366
1
1089
1113
BBa_K1980003
1
MymTsfGFP
MymT sfGFP
Sam Garforth
2016-10-10T11:00:00Z
2016-10-20T07:26:41Z
false
false
_2447_
29607
29607
9
false
MymT is a small prokaryotic copper metallothein discovered in Mycobacterium tuberculosis. It is believed that the protein may help the bacterium survive copper toxicity, though deleting the gene had no effect on pathogenicity in mice. It can bind up to 7 copper ions but has a preference for 4-6. This version has a C terminal sfGFP with a C terminal hexahistidine tag for purification. The MymT and sfGFP are separated by a small flexible linker. This part has been codon optimised for E. coli.
This protein has a C terminal hexahistidine tag for purification and has been codon optimised for E. coli.
Mycobacterium tuberculosis. Ordered as codon optimised DNA.
false
annotation2495245
1
sfGFP
range2495245
1
178
888
annotation2495248
1
Double stop
range2495248
1
906
912
annotation2495243
1
MymT
range2495243
1
1
159
annotation2495244
1
linker
range2495244
1
160
177
annotation2495246
1
Hexahistidine
range2495246
1
889
906
BBa_K1399014
1
BBa_K1399014
Plac-GFP(AAV)
Anna Stikane
2014-09-18T11:00:00Z
2015-05-08T01:10:16Z
false
false
_1777_
0
22477
9
In stock
true
Lactose/IPTG inducible promoter with GFP reporter tagged with AAV-ssrA degradation tag followed by terminator.
The tagged GFP is actively degraded within cell, thus provides better temporal resolution of green fluorescence and promoter activity.
Part was assembled using BrickClip assembly (BBF RFC104) using other biobrick parts as templates. BrickClip assembly is a special case of more general Paperclip assembly method.[1]
other biobricks
false
component2384055
1
BBa_K1399005
component2384049
1
BBa_B0034
component2384062
1
BBa_B0015
component2384041
1
BBa_R0010
annotation2384041
1
BBa_R0010
range2384041
1
1
200
annotation2384049
1
BBa_B0034
range2384049
1
209
220
annotation2384055
1
BBa_K1399005
range2384055
1
227
979
annotation2384062
1
BBa_B0015
range2384062
1
988
1116
BBa_J97001
1
BBa_J97001
P-Free JuniperGFP (Green Fluorescent Protein)
Drew Endy
2014-02-18T12:00:00Z
2015-05-08T01:08:32Z
false
false
_41_1_
0
747
63
Not in stock
false
This part was created by DNA 2.0 as part of their IP-Free series of fluorescent and chromogenic proteins. It is available to use under the BioBrick Public Agreement.
Excitation 508nm, Emission 521nm
Molecular Weight: 26.6kDA
Length: 237aa
This sequence is fully synthetic, (non-Aequorea) and is codon optimized for expression in E. coli.
This synthetic part was created through a gene engineering and synthesis project at DNA 2.0.
false