//cds/reporter/rfp
type: Collection
persistent identity: cds reporter rfp
display id: cds_reporter_rfp
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
owned by: james
owned by: myers
member: BBa_K1399011
member: BBa_K1673103
member: BBa_K1122005
member: mRFP
member: BBa_K1673102
member: BBa_K1799026
member: dTomato
member: BBa_K1122006
member: mCherryC
member: BBa_K1673104
member: mRFP1
member: BBa_K1351021
member: BBa_K1399010
member: BBa_K180008
member: BBa_K1641224
member: BBa_K1673101
member: BBa_K1673010
member: BBa_K106004
member: BBa_K1184000
member: BBa_K1480002
member: BBa_K2040122
member: BBa_J97003
member: BBa_K1323009
member: BBa_K1732025
member: BBa_K1926022
member: BBa_K1980007
member: BBa_K1510017
member: BBa_K106005
member: BBa_K1399013
member: RFP2
member: BBa_K1362461
member: mCherry
member: BBa_K1926031
member: BBa_K1399012
member: BBa_K1497021
member: BBa_K1980009
member: RFP1
member: BBa_K1926013

BBa_K1122005
type: ComponentDefinition
persistent identity: BBa K1122005
display id: BBa_K1122005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: dsRED (BBa_E1010) compatible with Genabler assembly
creator: Lina Gasiūnaitė
created: 2013-08-12T11:00:00Z
modified: 2016-01-26T02:16:37Z
discontinued: false
dominant: false
group u list: _1433_
m user id: 4206
owner id: 18510
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: dsRED (BBa_E1010) part with inward facing EarI recognition sites at the ends
mutable notes: To be compatible with Genabler assembly, the part has inward-facing EarI sites at the ends. When assembled by BioBrick assembly, five N-terminal residues MetAlaSerSerSer- and one C-terminal Ala residue will be added to the original sequence of BBa_E1010.
mutable provenance: dsRED is based on BBa_E1010
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: EarI site
sequence annotation: dsRED
sequence annotation: stop
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_K1122005_sequence/1

EarI site
type: SequenceAnnotation
persistent identity: annotation2334601
display id: annotation2334601
version: 1
top level: BBa_K1122005
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1122005/annotation2334601/range2334601/1

https://synbiohub.org/public/igem/BBa_K1122005/annotation2334601/range2334601/1
type: Range
persistent identity: range2334601
display id: range2334601
version: 1
direction: direction/forward
top level: BBa_K1122005
start: 6
end: 11
orientation: inline

dsRED
type: SequenceAnnotation
persistent identity: annotation2346297
display id: annotation2346297
version: 1
top level: BBa_K1122005
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1122005/annotation2346297/range2346297/1

https://synbiohub.org/public/igem/BBa_K1122005/annotation2346297/range2346297/1
type: Range
persistent identity: range2346297
display id: range2346297
version: 1
direction: direction/forward
top level: BBa_K1122005
start: 1
end: 697
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2334603
display id: annotation2334603
version: 1
top level: BBa_K1122005
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1122005/annotation2334603/range2334603/1

https://synbiohub.org/public/igem/BBa_K1122005/annotation2334603/range2334603/1
type: Range
persistent identity: range2334603
display id: range2334603
version: 1
direction: direction/forward
top level: BBa_K1122005
start: 691
end: 693
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2334600
display id: annotation2334600
version: 1
top level: BBa_K1122005
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1122005/annotation2334600/range2334600/1

https://synbiohub.org/public/igem/BBa_K1122005/annotation2334600/range2334600/1
type: Range
persistent identity: range2334600
display id: range2334600
version: 1
direction: direction/forward
top level: BBa_K1122005
start: 1
end: 3
orientation: inline

EarI site
type: SequenceAnnotation
persistent identity: annotation2334602
display id: annotation2334602
version: 1
top level: BBa_K1122005
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1122005/annotation2334602/range2334602/1

https://synbiohub.org/public/igem/BBa_K1122005/annotation2334602/range2334602/1
type: Range
persistent identity: range2334602
display id: range2334602
version: 1
direction: direction/reverse
top level: BBa_K1122005
start: 692
end: 697
orientation: inline

BBa_K1799026
type: ComponentDefinition
persistent identity: BBa K1799026
display id: BBa_K1799026
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Discosoma RFP with stp
creator: Ellen Jorgensen
created: 2015-09-17T11:00:00Z
modified: 2016-01-21T02:18:57Z
discontinued: false
dominant: false
group u list: _2224_
m user id: 4206
owner id: 9151
owning group id: 9
status: status/Planning
bookmark: false
mutable description: BBa_E1010 Discosoma RFP with stp
mutable notes: BBa_E1010 Discosoma RFP with stp
mutable provenance: BBa_E1010
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1799026_sequence/1

BBa_J97003
type: ComponentDefinition
persistent identity: BBa J97003
display id: BBa_J97003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: IP-Free TannenRFP (Red Fluorescent Protein)
creator: Drew Endy
created: 2014-02-18T12:00:00Z
modified: 2015-05-08T01:08:32Z
discontinued: false
dominant: false
experience: experience/None
group u list: _41_1_
m user id: 0
owner id: 747
owning group id: 63
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: x
mutable notes: This sequence is fully synthetic, (non-Aequorea) and is codon optimized for expression in E. coli.
mutable provenance: This synthetic part was created through a gene engineering and synthesis project at DNA 2.0.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence: https://synbiohub.org/public/igem/BBa_J97003_sequence/1

BBa_K1351021
type: ComponentDefinition
persistent identity: BBa K1351021
display id: BBa_K1351021
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Monomeric Red Fluorescent Protein from Discosoma striata
creator: Anna Sommer
created: 2014-09-29T11:00:00Z
modified: 2016-01-26T01:58:37Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1726_
m user id: 4206
owner id: 20043
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: Mutant of the Biobrick BBa_E1010, in order to make it compatible with the Freiburg-Standard, two AgeI-Restriction sites were deleted monomeric RFP: Red Fluorescent Protein. Excitation peak: 584 nm Emission peak: 607 nm
mutable notes: The Biobrick has the Following Prefix and Suffix: Gatcgaattccgcggccgcttctagataaggaggagccggc Gatcctgcagcggccgctactagtattaaccggt
mutable provenance: Discosoma striata (coral)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1351021_sequence/1

BBa_K1323009
type: ComponentDefinition
persistent identity: BBa K1323009
display id: BBa_K1323009
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: DsRed (RFP) Coding Sequence
creator: Julia Manalil
created: 2014-10-03T11:00:00Z
modified: 2016-01-26T02:07:51Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1698_
m user id: 4206
owner id: 18368
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: TBA
mutable notes: TBA
mutable provenance: TBA
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: DsRed
sequence: https://synbiohub.org/public/igem/BBa_K1323009_sequence/1

DsRed
type: SequenceAnnotation
persistent identity: annotation2428765
display id: annotation2428765
version: 1
top level: BBa_K1323009
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1323009/annotation2428765/range2428765/1

https://synbiohub.org/public/igem/BBa_K1323009/annotation2428765/range2428765/1
type: Range
persistent identity: range2428765
display id: range2428765
version: 1
direction: direction/forward
top level: BBa_K1323009
start: 1
end: 678
orientation: inline

BBa_K1641224
type: ComponentDefinition
persistent identity: BBa K1641224
display id: BBa_K1641224
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: mCherry-ssrA
creator: Jianheng Liu
created: 2015-09-13T11:00:00Z
modified: 2016-01-25T01:06:32Z
discontinued: false
dominant: false
group u list: _2058_
m user id: 4206
owner id: 23346
owning group id: 9
status: status/Planning
bookmark: false
mutable description: mCherry (BBa_J06504) with ssrA tag（BBa_M0052）
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1641224_sequence/1

BBa_K1980007
type: ComponentDefinition
persistent identity: BBa K1980007
display id: BBa_K1980007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pCusC mKate2
creator: Sam Garforth
created: 2016-10-10T11:00:00Z
modified: 2016-10-23T03:43:26Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2447_
m user id: 29607
owner id: 29607
owning group id: 9
status: status/Planning
bookmark: false
mutable description: pCusC mKate2. copper sensitive promoter with RFP reporter gene
mutable notes: Codon optimised.
mutable provenance: E. coli mainly
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: pCusC
sequence annotation: CusR binding
sequence annotation: RBS
sequence annotation: Stop
sequence annotation: -35
sequence annotation: mKate2
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_K1980007_sequence/1

pCusC
type: SequenceAnnotation
persistent identity: annotation2525389
display id: annotation2525389
version: 1
top level: BBa_K1980007
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1980007/annotation2525389/range2525389/1

https://synbiohub.org/public/igem/BBa_K1980007/annotation2525389/range2525389/1
type: Range
persistent identity: range2525389
display id: range2525389
version: 1
direction: direction/forward
top level: BBa_K1980007
start: 1
end: 98
orientation: inline

CusR binding
type: SequenceAnnotation
persistent identity: annotation2528662
display id: annotation2528662
version: 1
top level: BBa_K1980007
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1980007/annotation2528662/range2528662/1

https://synbiohub.org/public/igem/BBa_K1980007/annotation2528662/range2528662/1
type: Range
persistent identity: range2528662
display id: range2528662
version: 1
direction: direction/forward
top level: BBa_K1980007
start: 30
end: 51
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2528246
display id: annotation2528246
version: 1
top level: BBa_K1980007
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1980007/annotation2528246/range2528246/1

https://synbiohub.org/public/igem/BBa_K1980007/annotation2528246/range2528246/1
type: Range
persistent identity: range2528246
display id: range2528246
version: 1
direction: direction/forward
top level: BBa_K1980007
start: 111
end: 116
orientation: inline

Stop
type: SequenceAnnotation
persistent identity: annotation2528248
display id: annotation2528248
version: 1
top level: BBa_K1980007
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1980007/annotation2528248/range2528248/1

https://synbiohub.org/public/igem/BBa_K1980007/annotation2528248/range2528248/1
type: Range
persistent identity: range2528248
display id: range2528248
version: 1
direction: direction/forward
top level: BBa_K1980007
start: 819
end: 824
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2528244
display id: annotation2528244
version: 1
top level: BBa_K1980007
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1980007/annotation2528244/range2528244/1

https://synbiohub.org/public/igem/BBa_K1980007/annotation2528244/range2528244/1
type: Range
persistent identity: range2528244
display id: range2528244
version: 1
direction: direction/forward
top level: BBa_K1980007
start: 60
end: 65
orientation: inline

mKate2
type: SequenceAnnotation
persistent identity: annotation2528247
display id: annotation2528247
version: 1
top level: BBa_K1980007
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1980007/annotation2528247/range2528247/1

https://synbiohub.org/public/igem/BBa_K1980007/annotation2528247/range2528247/1
type: Range
persistent identity: range2528247
display id: range2528247
version: 1
direction: direction/forward
top level: BBa_K1980007
start: 126
end: 818
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2528245
display id: annotation2528245
version: 1
top level: BBa_K1980007
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1980007/annotation2528245/range2528245/1

https://synbiohub.org/public/igem/BBa_K1980007/annotation2528245/range2528245/1
type: Range
persistent identity: range2528245
display id: range2528245
version: 1
direction: direction/forward
top level: BBa_K1980007
start: 81
end: 87
orientation: inline

BBa_K1926022
type: ComponentDefinition
persistent identity: BBa K1926022
display id: BBa_K1926022
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: The SNAP-mCHERRY UNIT
creator: Xinyi Liu
created: 2016-10-09T11:00:00Z
modified: 2016-10-11T06:31:46Z
discontinued: false
dominant: false
group u list: _2393_
m user id: 28998
owner id: 28998
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This part is the SNAP-mCHERRY UNIT of cyclebow including a SNAP UNIT and a mCHERRY UNIT. The SNAP UNIT, which can be cut off by CRE, includes an sv40 nuclear location sequence (NLS), a covalent labeling of fusion proteins named SNAP-tag?? and an sv40 terminator flanked by homodromous recognition site of recombinase CRE named loxP. The mCHERRY UNIT, which can be cut off by VIKA, includes a degradation tag named DBOX, a fluorescent gene mCherry and an sv40 terminator flanked by homodromous recognition site of recombinase VIKA named Vox. You may use this part to: 1) Test the cut-off efficiency of recombinase CRE and VIKA; 2) Estimate the time needed for genome repair because mCherry can only be expressed after the cut off of SNAP UNIT and genome repair; 3) Fast cut off this part by transient transfecting recombinase gene into nucleus; 4) When there is no CRE, you can use this part as the SNAP UNIT, which can help localize proteins in vivo with BLOCK and dye from NEB company.
mutable notes: None
mutable provenance: The SNAP-tag?? was brought from NEB company. NLS and sv40 terminator were got from commercial plasmid: pentry nls GFP/pcdna3.1. The sequence of Vox and DBOX was retrieved from Addgene and got through denovo synthesis. mCHERRY was got from commercial plasmid: pentry dcas9 mcherry bira.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1926013
component: BBa_K1926011
sequence annotation: BBa_K1926013
sequence annotation: BBa_K1926011
sequence: https://synbiohub.org/public/igem/BBa_K1926022_sequence/1

BBa_K1926013
type: Component
persistent identity: component2491941
display id: component2491941
version: 1
top level: BBa_K1926022
access: public
definition: BBa_K1926013

BBa_K1926011
type: Component
persistent identity: component2491934
display id: component2491934
version: 1
top level: BBa_K1926022
access: public
definition: https://synbiohub.org/public/igem/BBa_K1926011/1

BBa_K1926013
type: SequenceAnnotation
persistent identity: annotation2491941
display id: annotation2491941
version: 1
top level: BBa_K1926022
location: https://synbiohub.org/public/igem/BBa_K1926022/annotation2491941/range2491941/1
component: BBa_K1926013

https://synbiohub.org/public/igem/BBa_K1926022/annotation2491941/range2491941/1
type: Range
persistent identity: range2491941
display id: range2491941
version: 1
direction: direction/reverse
top level: BBa_K1926022
start: 789
end: 1704
orientation: inline

BBa_K1926011
type: SequenceAnnotation
persistent identity: annotation2491934
display id: annotation2491934
version: 1
top level: BBa_K1926022
location: https://synbiohub.org/public/igem/BBa_K1926022/annotation2491934/range2491934/1
component: BBa_K1926011

https://synbiohub.org/public/igem/BBa_K1926022/annotation2491934/range2491934/1
type: Range
persistent identity: range2491934
display id: range2491934
version: 1
direction: direction/reverse
top level: BBa_K1926022
start: 1
end: 788
orientation: inline

BBa_K1480002
type: ComponentDefinition
persistent identity: BBa K1480002
display id: BBa_K1480002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pTet + RBS + mRFP1 + Transcriptional terminator
creator: Jesus Gilberto Rodriguez Ceja
created: 2014-10-07T11:00:00Z
modified: 2015-05-08T01:10:40Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1860_
m user id: 0
owner id: 12878
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part codifies for a red fluorescent protein (BBa_K081014). It??s regulated by a TetR repressible promoter (R0040).
mutable notes: Assembled using assembly 10.
mutable provenance: mRFP: Monomeric red fluorescent protein engineered to enhance photoresistance (BBa_E1010). It was used the part BBa_K081014. ptet: Based in the TetR repressible promoter of operon for resistance against tetracycline (BBa_R0040).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_E1010
component: BBa_B0030
component: BBa_B1006
component: BBa_R0040
sequence annotation: BBa_B1006
sequence annotation: BBa_R0040
sequence annotation: BBa_B0030
sequence annotation: BBa_E1010
sequence: https://synbiohub.org/public/igem/BBa_K1480002_sequence/1

BBa_E1010
type: Component
persistent identity: component2406226
display id: component2406226
version: 1
top level: BBa_K1480002
access: public
definition: https://synbiohub.org/public/igem/BBa_E1010/1

BBa_B0030
type: Component
persistent identity: component2406222
display id: component2406222
version: 1
top level: BBa_K1480002
access: public
definition: https://synbiohub.org/public/igem/BBa_B0030/1

BBa_B1006
type: Component
persistent identity: component2406231
display id: component2406231
version: 1
top level: BBa_K1480002
access: public
definition: https://synbiohub.org/public/igem/BBa_B1006/1

BBa_R0040
type: Component
persistent identity: component2406216
display id: component2406216
version: 1
top level: BBa_K1480002
access: public
definition: https://synbiohub.org/public/igem/BBa_R0040/1

BBa_B1006
type: SequenceAnnotation
persistent identity: annotation2406231
display id: annotation2406231
version: 1
top level: BBa_K1480002
location: https://synbiohub.org/public/igem/BBa_K1480002/annotation2406231/range2406231/1
component: BBa_B1006

https://synbiohub.org/public/igem/BBa_K1480002/annotation2406231/range2406231/1
type: Range
persistent identity: range2406231
display id: range2406231
version: 1
direction: direction/reverse
top level: BBa_K1480002
start: 798
end: 836
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation2406216
display id: annotation2406216
version: 1
top level: BBa_K1480002
location: https://synbiohub.org/public/igem/BBa_K1480002/annotation2406216/range2406216/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_K1480002/annotation2406216/range2406216/1
type: Range
persistent identity: range2406216
display id: range2406216
version: 1
direction: direction/forward
top level: BBa_K1480002
start: 1
end: 54
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation2406222
display id: annotation2406222
version: 1
top level: BBa_K1480002
location: https://synbiohub.org/public/igem/BBa_K1480002/annotation2406222/range2406222/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_K1480002/annotation2406222/range2406222/1
type: Range
persistent identity: range2406222
display id: range2406222
version: 1
direction: direction/forward
top level: BBa_K1480002
start: 63
end: 77
orientation: inline

BBa_E1010
type: SequenceAnnotation
persistent identity: annotation2406226
display id: annotation2406226
version: 1
top level: BBa_K1480002
location: https://synbiohub.org/public/igem/BBa_K1480002/annotation2406226/range2406226/1
component: BBa_E1010

https://synbiohub.org/public/igem/BBa_K1480002/annotation2406226/range2406226/1
type: Range
persistent identity: range2406226
display id: range2406226
version: 1
direction: direction/reverse
top level: BBa_K1480002
start: 84
end: 789
orientation: inline

BBa_K1673102
type: ComponentDefinition
persistent identity: BBa K1673102
display id: BBa_K1673102
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: UV Mutation Optimized RFP (Improved Expression)
creator: Jarrod Shilts
created: 2015-09-16T11:00:00Z
modified: 2016-01-21T04:04:00Z
discontinued: false
dominant: false
group u list: _2091_
m user id: 4206
owner id: 19415
owning group id: 9
status: status/Planning
bookmark: false
mutable description: RFP sequence, based on E1010, with the number of possible pyrimidine dimer-forming sites reduced to the maximum possible extent. Altered from BBa_K1673101 to increase effective expression approximately 40%
mutable notes: Sequence produced by the Vanderbilt iGEM 2015 mutation optimization software.
mutable provenance: Synthesized DNA
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1673102_sequence/1

BBa_K180008
type: ComponentDefinition
persistent identity: BBa K180008
display id: BBa_K180008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: mCherry (rights owned by Clontech)
creator: Richard Wilson, Stephanie Chambers
created: 2009-10-15T11:00:00Z
modified: 2016-01-26T10:07:05Z
discontinued: false
dominant: false
experience: experience/None
group u list: _278_
m user id: 4206
owner id: 4082
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: mCherry fluorescent protein - Rights owned by Clontech. Sequence is commercially available from Clontech. Sequence obtained through PCR amplification, with BioBrick prefix and suffix added through in-house designed primers.
mutable notes: PstI site within the mCherry sequence - has to be assembled using BioBrick assembly in such an order that any successive digestions will not require PstI, otherwise the mCherry sequence will be cut as well as the site of interest.
mutable provenance: pmCherry vector, Clontech
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: mCherry
sequence: https://synbiohub.org/public/igem/BBa_K180008_sequence/1

mCherry
type: SequenceAnnotation
persistent identity: annotation2046808
display id: annotation2046808
version: 1
top level: BBa_K180008
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K180008/annotation2046808/range2046808/1

https://synbiohub.org/public/igem/BBa_K180008/annotation2046808/range2046808/1
type: Range
persistent identity: range2046808
display id: range2046808
version: 1
direction: direction/forward
top level: BBa_K180008
start: 1
end: 708
orientation: inline

RFP1
type: ComponentDefinition
persistent identity: BBa I715022
display id: BBa_I715022
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Amino Portion of RFP
creator: Andrew Martens
created: 2007-06-24T11:00:00Z
modified: 2016-01-25T04:43:58Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _120_
m user id: 4206
owner id: 201
owning group id: 61
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: af
mutable notes: afd
mutable provenance: adf
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: start
sequence annotation: Coding sequence
sequence: https://synbiohub.org/public/igem/BBa_I715022_sequence/1

start
type: SequenceAnnotation
persistent identity: annotation1935380
display id: annotation1935380
version: 1
top level: RFP1
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_I715022/annotation1935380/range1935380/1

https://synbiohub.org/public/igem/BBa_I715022/annotation1935380/range1935380/1
type: Range
persistent identity: range1935380
display id: range1935380
version: 1
direction: direction/forward
top level: RFP1
start: 1
end: 3
orientation: inline

Coding sequence
type: SequenceAnnotation
persistent identity: annotation1935381
display id: annotation1935381
version: 1
top level: RFP1
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_I715022/annotation1935381/range1935381/1

https://synbiohub.org/public/igem/BBa_I715022/annotation1935381/range1935381/1
type: Range
persistent identity: range1935381
display id: range1935381
version: 1
direction: direction/forward
top level: RFP1
start: 1
end: 462
orientation: inline

BBa_K1399013
type: ComponentDefinition
persistent identity: BBa K1399013
display id: BBa_K1399013
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Plac-RFP(DAS)
creator: Anna Stikane
created: 2014-09-18T11:00:00Z
modified: 2015-05-08T01:10:16Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1777_
m user id: 0
owner id: 22477
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: Lactose/IPTG inducible promoter with RFP reporter tagged with DAS-ssrA degradation tag followed by terminator. The tagged RFP is degraded within cell, thus provides better temporal resolution of red fluorescence and promoter activity. Presence of SspB is crucial for effective protein degradation.
mutable notes: Part was assembled using BrickClip assembly (BBF RFC104) using other biobrick parts as templates. BrickClip assembly is a special case of more general Paperclip assembly method.[1]
mutable provenance: other biobricks
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: BBa_R0010
sequence annotation: stem_loop
sequence annotation: BBa_K1399003
sequence annotation: conserved
sequence annotation: end of LacI coding region (inactive)
sequence annotation: LacI binding site
sequence annotation: BBa_B0012
sequence annotation: DAS-ssrA tag
sequence annotation: start
sequence annotation: T7 TE
sequence annotation: stop
sequence annotation: CAP binding site
sequence annotation: RFP
sequence annotation: -35
sequence annotation: barcode from BBa_E1010
sequence annotation: polya
sequence annotation: BBa_B0010
sequence annotation: BBa_B0015
sequence annotation: -10
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K1399013_sequence/1

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation2384012
display id: annotation2384012
version: 1
top level: BBa_K1399013
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1399013/annotation2384012/range2384012/1

https://synbiohub.org/public/igem/BBa_K1399013/annotation2384012/range2384012/1
type: Range
persistent identity: range2384012
display id: range2384012
version: 1
direction: direction/forward
top level: BBa_K1399013
start: 1
end: 200
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation2384028
display id: annotation2384028
version: 1
top level: BBa_K1399013
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1399013/annotation2384028/range2384028/1

https://synbiohub.org/public/igem/BBa_K1399013/annotation2384028/range2384028/1
type: Range
persistent identity: range2384028
display id: range2384028
version: 1
direction: direction/forward
top level: BBa_K1399013
start: 985
end: 1028
orientation: inline

BBa_K1399003
type: SequenceAnnotation
persistent identity: annotation2384026
display id: annotation2384026
version: 1
top level: BBa_K1399013
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1399013/annotation2384026/range2384026/1

https://synbiohub.org/public/igem/BBa_K1399013/annotation2384026/range2384026/1
type: Range
persistent identity: range2384026
display id: range2384026
version: 1
direction: direction/reverse
top level: BBa_K1399013
start: 227
end: 940
orientation: inline

conserved
type: SequenceAnnotation
persistent identity: annotation2384019
display id: annotation2384019
version: 1
top level: BBa_K1399013
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K1399013/annotation2384019/range2384019/1

https://synbiohub.org/public/igem/BBa_K1399013/annotation2384019/range2384019/1
type: Range
persistent identity: range2384019
display id: range2384019
version: 1
direction: direction/forward
top level: BBa_K1399013
start: 213
end: 216
orientation: inline

end of LacI coding region (inactive)
type: SequenceAnnotation
persistent identity: annotation2384013
display id: annotation2384013
version: 1
top level: BBa_K1399013
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1399013/annotation2384013/range2384013/1

https://synbiohub.org/public/igem/BBa_K1399013/annotation2384013/range2384013/1
type: Range
persistent identity: range2384013
display id: range2384013
version: 1
direction: direction/forward
top level: BBa_K1399013
start: 1
end: 88
orientation: inline

LacI binding site
type: SequenceAnnotation
persistent identity: annotation2384017
display id: annotation2384017
version: 1
top level: BBa_K1399013
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1399013/annotation2384017/range2384017/1

https://synbiohub.org/public/igem/BBa_K1399013/annotation2384017/range2384017/1
type: Range
persistent identity: range2384017
display id: range2384017
version: 1
direction: direction/forward
top level: BBa_K1399013
start: 166
end: 200
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2384029
display id: annotation2384029
version: 1
top level: BBa_K1399013
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1399013/annotation2384029/range2384029/1

https://synbiohub.org/public/igem/BBa_K1399013/annotation2384029/range2384029/1
type: Range
persistent identity: range2384029
display id: range2384029
version: 1
direction: direction/forward
top level: BBa_K1399013
start: 1062
end: 1102
orientation: inline

DAS-ssrA tag
type: SequenceAnnotation
persistent identity: annotation2384023
display id: annotation2384023
version: 1
top level: BBa_K1399013
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1399013/annotation2384023/range2384023/1

https://synbiohub.org/public/igem/BBa_K1399013/annotation2384023/range2384023/1
type: Range
persistent identity: range2384023
display id: range2384023
version: 1
direction: direction/forward
top level: BBa_K1399013
start: 902
end: 934
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2384021
display id: annotation2384021
version: 1
top level: BBa_K1399013
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1399013/annotation2384021/range2384021/1

https://synbiohub.org/public/igem/BBa_K1399013/annotation2384021/range2384021/1
type: Range
persistent identity: range2384021
display id: range2384021
version: 1
direction: direction/forward
top level: BBa_K1399013
start: 227
end: 229
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation2384030
display id: annotation2384030
version: 1
top level: BBa_K1399013
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1399013/annotation2384030/range2384030/1

https://synbiohub.org/public/igem/BBa_K1399013/annotation2384030/range2384030/1
type: Range
persistent identity: range2384030
display id: range2384030
version: 1
direction: direction/forward
top level: BBa_K1399013
start: 1069
end: 1088
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2384018
display id: annotation2384018
version: 1
top level: BBa_K1399013
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1399013/annotation2384018/range2384018/1

https://synbiohub.org/public/igem/BBa_K1399013/annotation2384018/range2384018/1
type: Range
persistent identity: range2384018
display id: range2384018
version: 1
direction: direction/forward
top level: BBa_K1399013
start: 173
end: 173
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2384032
display id: annotation2384032
version: 1
top level: BBa_K1399013
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1399013/annotation2384032/range2384032/1

https://synbiohub.org/public/igem/BBa_K1399013/annotation2384032/range2384032/1
type: Range
persistent identity: range2384032
display id: range2384032
version: 1
direction: direction/forward
top level: BBa_K1399013
start: 1095
end: 1095
orientation: inline

CAP binding site
type: SequenceAnnotation
persistent identity: annotation2384014
display id: annotation2384014
version: 1
top level: BBa_K1399013
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1399013/annotation2384014/range2384014/1

https://synbiohub.org/public/igem/BBa_K1399013/annotation2384014/range2384014/1
type: Range
persistent identity: range2384014
display id: range2384014
version: 1
direction: direction/forward
top level: BBa_K1399013
start: 89
end: 126
orientation: inline

RFP
type: SequenceAnnotation
persistent identity: annotation2384022
display id: annotation2384022
version: 1
top level: BBa_K1399013
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1399013/annotation2384022/range2384022/1

https://synbiohub.org/public/igem/BBa_K1399013/annotation2384022/range2384022/1
type: Range
persistent identity: range2384022
display id: range2384022
version: 1
direction: direction/forward
top level: BBa_K1399013
start: 230
end: 901
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2384015
display id: annotation2384015
version: 1
top level: BBa_K1399013
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1399013/annotation2384015/range2384015/1

https://synbiohub.org/public/igem/BBa_K1399013/annotation2384015/range2384015/1
type: Range
persistent identity: range2384015
display id: range2384015
version: 1
direction: direction/forward
top level: BBa_K1399013
start: 137
end: 142
orientation: inline

barcode from BBa_E1010
type: SequenceAnnotation
persistent identity: annotation2386313
display id: annotation2386313
version: 1
top level: BBa_K1399013
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1399013/annotation2386313/range2386313/1

https://synbiohub.org/public/igem/BBa_K1399013/annotation2386313/range2386313/1
type: Range
persistent identity: range2386313
display id: range2386313
version: 1
direction: direction/forward
top level: BBa_K1399013
start: 941
end: 965
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2384025
display id: annotation2384025
version: 1
top level: BBa_K1399013
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1399013/annotation2384025/range2384025/1

https://synbiohub.org/public/igem/BBa_K1399013/annotation2384025/range2384025/1
type: Range
persistent identity: range2384025
display id: range2384025
version: 1
direction: direction/forward
top level: BBa_K1399013
start: 938
end: 940
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2384024
display id: annotation2384024
version: 1
top level: BBa_K1399013
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1399013/annotation2384024/range2384024/1

https://synbiohub.org/public/igem/BBa_K1399013/annotation2384024/range2384024/1
type: Range
persistent identity: range2384024
display id: range2384024
version: 1
direction: direction/forward
top level: BBa_K1399013
start: 935
end: 937
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation2384031
display id: annotation2384031
version: 1
top level: BBa_K1399013
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_K1399013/annotation2384031/range2384031/1

https://synbiohub.org/public/igem/BBa_K1399013/annotation2384031/range2384031/1
type: Range
persistent identity: range2384031
display id: range2384031
version: 1
direction: direction/forward
top level: BBa_K1399013
start: 1089
end: 1102
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2384027
display id: annotation2384027
version: 1
top level: BBa_K1399013
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1399013/annotation2384027/range2384027/1

https://synbiohub.org/public/igem/BBa_K1399013/annotation2384027/range2384027/1
type: Range
persistent identity: range2384027
display id: range2384027
version: 1
direction: direction/forward
top level: BBa_K1399013
start: 974
end: 1053
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2384033
display id: annotation2384033
version: 1
top level: BBa_K1399013
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1399013/annotation2384033/range2384033/1

https://synbiohub.org/public/igem/BBa_K1399013/annotation2384033/range2384033/1
type: Range
persistent identity: range2384033
display id: range2384033
version: 1
direction: direction/reverse
top level: BBa_K1399013
start: 974
end: 1102
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2384016
display id: annotation2384016
version: 1
top level: BBa_K1399013
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1399013/annotation2384016/range2384016/1

https://synbiohub.org/public/igem/BBa_K1399013/annotation2384016/range2384016/1
type: Range
persistent identity: range2384016
display id: range2384016
version: 1
direction: direction/forward
top level: BBa_K1399013
start: 161
end: 166
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2384020
display id: annotation2384020
version: 1
top level: BBa_K1399013
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1399013/annotation2384020/range2384020/1

https://synbiohub.org/public/igem/BBa_K1399013/annotation2384020/range2384020/1
type: Range
persistent identity: range2384020
display id: range2384020
version: 1
direction: direction/reverse
top level: BBa_K1399013
start: 209
end: 220
orientation: inline

BBa_K1362461
type: ComponentDefinition
persistent identity: BBa K1362461
display id: BBa_K1362461
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: **highly** engineered mutant of red fluorescent protein from Discosoma striata (coral) with barcode
creator: Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena Büscher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Schäfer, Carolin Schmelas, Silvan Schmitz, Max Waldha
created: 2014-10-07T11:00:00Z
modified: 2016-01-25T01:30:37Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1738_
m user id: 4206
owner id: 22951
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This part is essentially identical to <partinfo>BBa_E1010</partinfo>, but has a point mutation in the barcode. Barcodes are discontinued, but composite parts using this part will have the mutated barcode.
mutable provenance: ''Discosoma striata''
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: mrfp1
sequence annotation: mutated barcode
sequence: https://synbiohub.org/public/igem/BBa_K1362461_sequence/1

mrfp1
type: SequenceAnnotation
persistent identity: annotation2404599
display id: annotation2404599
version: 1
top level: BBa_K1362461
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1362461/annotation2404599/range2404599/1

https://synbiohub.org/public/igem/BBa_K1362461/annotation2404599/range2404599/1
type: Range
persistent identity: range2404599
display id: range2404599
version: 1
direction: direction/forward
top level: BBa_K1362461
start: 1
end: 675
orientation: inline

mutated barcode
type: SequenceAnnotation
persistent identity: annotation2404604
display id: annotation2404604
version: 1
top level: BBa_K1362461
role: SO:0000807
role: feature/barcode
location: https://synbiohub.org/public/igem/BBa_K1362461/annotation2404604/range2404604/1

https://synbiohub.org/public/igem/BBa_K1362461/annotation2404604/range2404604/1
type: Range
persistent identity: range2404604
display id: range2404604
version: 1
direction: direction/forward
top level: BBa_K1362461
start: 682
end: 706
orientation: inline

BBa_K1673104
type: ComponentDefinition
persistent identity: BBa K1673104
display id: BBa_K1673104
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Oxidation-Resistant RFP
creator: Jarrod Shilts
created: 2015-09-16T11:00:00Z
modified: 2016-01-21T04:03:04Z
discontinued: false
dominant: false
group u list: _2091_
m user id: 4206
owner id: 19415
owning group id: 9
status: status/Planning
bookmark: false
mutable description: RFP sequence, based on E1010, with the number of oxidation-prone sites reduced to the minimum possible amount.
mutable notes: Sequence produced by the Vanderbilt iGEM 2015 mutation optimization software.
mutable provenance: Synthesized DNA
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1673104_sequence/1

BBa_K106005
type: ComponentDefinition
persistent identity: BBa K106005
display id: BBa_K106005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: mCherry, Aar1 BD part
creator: Ryan Quan
created: 2008-10-21T11:00:00Z
modified: 2016-01-26T09:49:02Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _226_
m user id: 4206
owner id: 3421
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This is the coding sequence for mCherry with AB ends for Aar1 cloning.
mutable notes: Since this is an BD part, there is no ATG but there is a stop codon. For more information on AarI cloning, please refer to the UCSF 2008 igem page.
mutable provenance: none
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K106005_sequence/1

dTomato
type: ComponentDefinition
persistent identity: BBa K1688010
display id: BBa_K1688010
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: dTomato (inc RBS, J23101 promoter)
creator: Dianna Zeleskov
created: 2015-09-14T11:00:00Z
modified: 2015-09-18T11:41:03Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2106_
m user id: 27796
owner id: 27121
owning group id: 9
status: status/Planning
bookmark: false
mutable description: The dTomato protein is a fluorescent dimer protein that emits orange-red light when it is excited by green-yellow light. It is preferable to use ??? especially in self-made fluorometry tests ??? because the excitation wavelengths and the emission wavelengths do not overlap as much as in other fluorescent proteins. The dTomato excitation peak is at 554 nm and 50% of it is at 510 nm. The emission peak is at 581 nm and the 50% emission peak is at 629 nm. Characterization has been made with the Anderson promoters BBa_J23101 (this construct) and BBa_J23110 (construct BBa_K1688011)
mutable provenance: dTomato was derived from a monomeric mutant of the anthozoan coral RFP, DsRed.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_J23101
component: BBa_K1688004
sequence annotation: BBa_K1688004
sequence annotation: BBa_J23101
sequence: https://synbiohub.org/public/igem/BBa_K1688010_sequence/1

BBa_J23101
type: Component
persistent identity: component2457518
display id: component2457518
version: 1
top level: dTomato
access: public
definition: https://synbiohub.org/public/igem/BBa_J23101/1

BBa_K1688004
type: Component
persistent identity: component2457522
display id: component2457522
version: 1
top level: dTomato
access: public
definition: dTomato

BBa_K1688004
type: SequenceAnnotation
persistent identity: annotation2457522
display id: annotation2457522
version: 1
top level: dTomato
location: https://synbiohub.org/public/igem/BBa_K1688010/annotation2457522/range2457522/1
component: BBa_K1688004

https://synbiohub.org/public/igem/BBa_K1688010/annotation2457522/range2457522/1
type: Range
persistent identity: range2457522
display id: range2457522
version: 1
direction: direction/reverse
top level: dTomato
start: 44
end: 769
orientation: inline

BBa_J23101
type: SequenceAnnotation
persistent identity: annotation2457518
display id: annotation2457518
version: 1
top level: dTomato
location: https://synbiohub.org/public/igem/BBa_K1688010/annotation2457518/range2457518/1
component: BBa_J23101

https://synbiohub.org/public/igem/BBa_K1688010/annotation2457518/range2457518/1
type: Range
persistent identity: range2457518
display id: range2457518
version: 1
direction: direction/reverse
top level: dTomato
start: 1
end: 35
orientation: inline

mCherry
type: ComponentDefinition
persistent identity: BBa J06504
display id: BBa_J06504
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: monomeric RFP optimized for bacteria
creator: ytwang
created: 2005-07-17T11:00:00Z
modified: 2016-01-25T01:05:28Z
discontinued: false
dominant: false
experience: experience/None
group u list: _20_
m user id: 4206
owner id: 340
owning group id: 20
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: mRFP (DsRed) derived, altered to be a biobrick by removing a PstI site and adding in the ends.
mutable notes: Made a point mutation to eliminate a PstI site in the middle and then added BioBrick ends using PCR. Sequenced using primers that bind to the pSB1A2 plasmid on either side of the brick.
mutable provenance: mCherry from Roger Y. Tsien's lab, altered to be BioBrick compatible
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: mCherry
sequence annotation: C->T (removing PstI site)
sequence: https://synbiohub.org/public/igem/BBa_J06504_sequence/1

mCherry
type: SequenceAnnotation
persistent identity: annotation1585829
display id: annotation1585829
version: 1
top level: mCherry
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J06504/annotation1585829/range1585829/1

https://synbiohub.org/public/igem/BBa_J06504/annotation1585829/range1585829/1
type: Range
persistent identity: range1585829
display id: range1585829
version: 1
direction: direction/forward
top level: mCherry
start: 1
end: 711
orientation: inline

C->T (removing PstI site)
type: SequenceAnnotation
persistent identity: annotation1585833
display id: annotation1585833
version: 1
top level: mCherry
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_J06504/annotation1585833/range1585833/1

https://synbiohub.org/public/igem/BBa_J06504/annotation1585833/range1585833/1
type: Range
persistent identity: range1585833
display id: range1585833
version: 1
direction: direction/forward
top level: mCherry
start: 352
end: 352
orientation: inline

BBa_K1732025
type: ComponentDefinition
persistent identity: BBa K1732025
display id: BBa_K1732025
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RFPCO-His
creator: Donna Lee
created: 2015-09-17T11:00:00Z
modified: 2016-01-21T02:30:37Z
discontinued: false
dominant: false
group u list: _2154_
m user id: 4206
owner id: 27195
owning group id: 9
status: status/Planning
bookmark: false
mutable description: RFP (BBa_1491008) with 6X His Tag for easier protein purification. Original sequence was codon optimized for E.coli by IDT Codon optimization tool.
mutable notes: His tag for easier protein purification and codon optimized for E.coli.
mutable provenance: BBa_1491008
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: 6X-His
sequence annotation: RFPCO
sequence: https://synbiohub.org/public/igem/BBa_K1732025_sequence/1

6X-His
type: SequenceAnnotation
persistent identity: annotation2469439
display id: annotation2469439
version: 1
top level: BBa_K1732025
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1732025/annotation2469439/range2469439/1

https://synbiohub.org/public/igem/BBa_K1732025/annotation2469439/range2469439/1
type: Range
persistent identity: range2469439
display id: range2469439
version: 1
direction: direction/forward
top level: BBa_K1732025
start: 672
end: 690
orientation: inline

RFPCO
type: SequenceAnnotation
persistent identity: annotation2469438
display id: annotation2469438
version: 1
top level: BBa_K1732025
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1732025/annotation2469438/range2469438/1

https://synbiohub.org/public/igem/BBa_K1732025/annotation2469438/range2469438/1
type: Range
persistent identity: range2469438
display id: range2469438
version: 1
direction: direction/forward
top level: BBa_K1732025
start: 1
end: 671
orientation: inline

dTomato
type: ComponentDefinition
persistent identity: BBa K1688019
display id: BBa_K1688019
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: dTomato, red fluorescent protein
creator: Erik Gullberg
created: 2015-09-14T11:00:00Z
modified: 2015-09-15T12:53:18Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2106_
m user id: 27118
owner id: 27118
owning group id: 9
status: status/Planning
bookmark: false
mutable description: The dTomato protein is a fluorescent dimer protein that emits orange-red light when it is excited by green-yellow light. It is preferable to use ??? especially in self-made fluorometry tests ??? because the excitation wavelengths and the emission wavelengths do not overlap as much as in other fluorescent proteins. The dTomato excitation peak is at 554 nm and 50% of it is at 510 nm. The emission peak is at 581 nm and the 50% emission peak is at 629 nm.
mutable provenance: dTomato was derived from a monomeric mutant of the anthozoan coral RFP, DsRed.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1688019_sequence/1

BBa_K1673010
type: ComponentDefinition
persistent identity: BBa K1673010
display id: BBa_K1673010
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Partially UV-Resistant RFP
creator: Jarrod Shilts
created: 2015-09-16T11:00:00Z
modified: 2016-01-21T04:17:27Z
discontinued: false
dominant: false
group u list: _2091_
m user id: 4206
owner id: 19415
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Partially optimized RFP sequence, based on E1010. Has a 4% reduction in the number of possible pyrimidine dimer-forming sites.
mutable notes: Sequence produced by the Vanderbilt iGEM 2015 mutation optimization software.
mutable provenance: Synthesized DNA
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1673010_sequence/1

BBa_K1673101
type: ComponentDefinition
persistent identity: BBa K1673101
display id: BBa_K1673101
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: UV-Resistant RFP
creator: Jarrod Shilts
created: 2015-09-16T11:00:00Z
modified: 2016-01-21T04:04:18Z
discontinued: false
dominant: false
group u list: _2091_
m user id: 4206
owner id: 19415
owning group id: 9
status: status/Planning
bookmark: false
mutable description: RFP sequence, based on E1010, with the number of possible pyrimidine dimer-forming sites reduced to the maximum possible extent.
mutable notes: Sequence produced by the Vanderbilt iGEM 2015 mutation optimization software.
mutable provenance: Synthesized DNA
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1673101_sequence/1

BBa_K1399012
type: ComponentDefinition
persistent identity: BBa K1399012
display id: BBa_K1399012
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Plac-RFP(LAA)
creator: Anna Stikane
created: 2014-09-18T11:00:00Z
modified: 2015-05-08T01:10:16Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1777_
m user id: 0
owner id: 22477
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: Lactose/IPTG inducible promoter with RBS, RFP reporter tagged with LAA-ssrA degradation tag and terminator. The tagged RFP is actively degraded within cell, thus provides better temporal resolution of red fluorescence and promoter activity.
mutable notes: Part was assembled using BrickClip assembly (BBF RFC 104) using other biobrick parts as templates. BrickClip assembly is a special case of more general Paperclip assembly method.[1]
mutable provenance: other biobricks
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: BBa_B0034
sequence annotation: polya
sequence annotation: BBa_B0010
sequence annotation: stop
sequence annotation: -10
sequence annotation: start
sequence annotation: T7 TE
sequence annotation: tag
sequence annotation: BBa_K1399002
sequence annotation: conserved
sequence annotation: -35
sequence annotation: stem_loop
sequence annotation: RFP
sequence annotation: barcode from BBa_E1010
sequence annotation: CAP binding site
sequence annotation: end of LacI coding region (inactive)
sequence annotation: BBa_B0012
sequence annotation: LacI binding site
sequence annotation: BBa_B0015
sequence annotation: BBa_R0010
sequence: https://synbiohub.org/public/igem/BBa_K1399012_sequence/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2383994
display id: annotation2383994
version: 1
top level: BBa_K1399012
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1399012/annotation2383994/range2383994/1

https://synbiohub.org/public/igem/BBa_K1399012/annotation2383994/range2383994/1
type: Range
persistent identity: range2383994
display id: range2383994
version: 1
direction: direction/reverse
top level: BBa_K1399012
start: 209
end: 220
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation2384005
display id: annotation2384005
version: 1
top level: BBa_K1399012
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_K1399012/annotation2384005/range2384005/1

https://synbiohub.org/public/igem/BBa_K1399012/annotation2384005/range2384005/1
type: Range
persistent identity: range2384005
display id: range2384005
version: 1
direction: direction/forward
top level: BBa_K1399012
start: 1089
end: 1102
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2384001
display id: annotation2384001
version: 1
top level: BBa_K1399012
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1399012/annotation2384001/range2384001/1

https://synbiohub.org/public/igem/BBa_K1399012/annotation2384001/range2384001/1
type: Range
persistent identity: range2384001
display id: range2384001
version: 1
direction: direction/forward
top level: BBa_K1399012
start: 974
end: 1053
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2383998
display id: annotation2383998
version: 1
top level: BBa_K1399012
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1399012/annotation2383998/range2383998/1

https://synbiohub.org/public/igem/BBa_K1399012/annotation2383998/range2383998/1
type: Range
persistent identity: range2383998
display id: range2383998
version: 1
direction: direction/forward
top level: BBa_K1399012
start: 935
end: 937
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2383990
display id: annotation2383990
version: 1
top level: BBa_K1399012
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1399012/annotation2383990/range2383990/1

https://synbiohub.org/public/igem/BBa_K1399012/annotation2383990/range2383990/1
type: Range
persistent identity: range2383990
display id: range2383990
version: 1
direction: direction/forward
top level: BBa_K1399012
start: 161
end: 166
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2383992
display id: annotation2383992
version: 1
top level: BBa_K1399012
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1399012/annotation2383992/range2383992/1

https://synbiohub.org/public/igem/BBa_K1399012/annotation2383992/range2383992/1
type: Range
persistent identity: range2383992
display id: range2383992
version: 1
direction: direction/forward
top level: BBa_K1399012
start: 173
end: 173
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation2384004
display id: annotation2384004
version: 1
top level: BBa_K1399012
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1399012/annotation2384004/range2384004/1

https://synbiohub.org/public/igem/BBa_K1399012/annotation2384004/range2384004/1
type: Range
persistent identity: range2384004
display id: range2384004
version: 1
direction: direction/forward
top level: BBa_K1399012
start: 1069
end: 1088
orientation: inline

tag
type: SequenceAnnotation
persistent identity: annotation2383997
display id: annotation2383997
version: 1
top level: BBa_K1399012
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1399012/annotation2383997/range2383997/1

https://synbiohub.org/public/igem/BBa_K1399012/annotation2383997/range2383997/1
type: Range
persistent identity: range2383997
display id: range2383997
version: 1
direction: direction/forward
top level: BBa_K1399012
start: 902
end: 934
orientation: inline

BBa_K1399002
type: SequenceAnnotation
persistent identity: annotation2384000
display id: annotation2384000
version: 1
top level: BBa_K1399012
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1399012/annotation2384000/range2384000/1

https://synbiohub.org/public/igem/BBa_K1399012/annotation2384000/range2384000/1
type: Range
persistent identity: range2384000
display id: range2384000
version: 1
direction: direction/reverse
top level: BBa_K1399012
start: 227
end: 940
orientation: inline

conserved
type: SequenceAnnotation
persistent identity: annotation2383993
display id: annotation2383993
version: 1
top level: BBa_K1399012
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K1399012/annotation2383993/range2383993/1

https://synbiohub.org/public/igem/BBa_K1399012/annotation2383993/range2383993/1
type: Range
persistent identity: range2383993
display id: range2383993
version: 1
direction: direction/forward
top level: BBa_K1399012
start: 213
end: 216
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2383989
display id: annotation2383989
version: 1
top level: BBa_K1399012
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1399012/annotation2383989/range2383989/1

https://synbiohub.org/public/igem/BBa_K1399012/annotation2383989/range2383989/1
type: Range
persistent identity: range2383989
display id: range2383989
version: 1
direction: direction/forward
top level: BBa_K1399012
start: 137
end: 142
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2383999
display id: annotation2383999
version: 1
top level: BBa_K1399012
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1399012/annotation2383999/range2383999/1

https://synbiohub.org/public/igem/BBa_K1399012/annotation2383999/range2383999/1
type: Range
persistent identity: range2383999
display id: range2383999
version: 1
direction: direction/forward
top level: BBa_K1399012
start: 938
end: 940
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation2384002
display id: annotation2384002
version: 1
top level: BBa_K1399012
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1399012/annotation2384002/range2384002/1

https://synbiohub.org/public/igem/BBa_K1399012/annotation2384002/range2384002/1
type: Range
persistent identity: range2384002
display id: range2384002
version: 1
direction: direction/forward
top level: BBa_K1399012
start: 985
end: 1028
orientation: inline

RFP
type: SequenceAnnotation
persistent identity: annotation2383996
display id: annotation2383996
version: 1
top level: BBa_K1399012
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1399012/annotation2383996/range2383996/1

https://synbiohub.org/public/igem/BBa_K1399012/annotation2383996/range2383996/1
type: Range
persistent identity: range2383996
display id: range2383996
version: 1
direction: direction/forward
top level: BBa_K1399012
start: 230
end: 901
orientation: inline

barcode from BBa_E1010
type: SequenceAnnotation
persistent identity: annotation2386312
display id: annotation2386312
version: 1
top level: BBa_K1399012
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1399012/annotation2386312/range2386312/1

https://synbiohub.org/public/igem/BBa_K1399012/annotation2386312/range2386312/1
type: Range
persistent identity: range2386312
display id: range2386312
version: 1
direction: direction/forward
top level: BBa_K1399012
start: 941
end: 965
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2384006
display id: annotation2384006
version: 1
top level: BBa_K1399012
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1399012/annotation2384006/range2384006/1

https://synbiohub.org/public/igem/BBa_K1399012/annotation2384006/range2384006/1
type: Range
persistent identity: range2384006
display id: range2384006
version: 1
direction: direction/forward
top level: BBa_K1399012
start: 1095
end: 1095
orientation: inline

CAP binding site
type: SequenceAnnotation
persistent identity: annotation2383988
display id: annotation2383988
version: 1
top level: BBa_K1399012
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1399012/annotation2383988/range2383988/1

https://synbiohub.org/public/igem/BBa_K1399012/annotation2383988/range2383988/1
type: Range
persistent identity: range2383988
display id: range2383988
version: 1
direction: direction/forward
top level: BBa_K1399012
start: 89
end: 126
orientation: inline

end of LacI coding region (inactive)
type: SequenceAnnotation
persistent identity: annotation2383987
display id: annotation2383987
version: 1
top level: BBa_K1399012
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1399012/annotation2383987/range2383987/1

https://synbiohub.org/public/igem/BBa_K1399012/annotation2383987/range2383987/1
type: Range
persistent identity: range2383987
display id: range2383987
version: 1
direction: direction/forward
top level: BBa_K1399012
start: 1
end: 88
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2384003
display id: annotation2384003
version: 1
top level: BBa_K1399012
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1399012/annotation2384003/range2384003/1

https://synbiohub.org/public/igem/BBa_K1399012/annotation2384003/range2384003/1
type: Range
persistent identity: range2384003
display id: range2384003
version: 1
direction: direction/forward
top level: BBa_K1399012
start: 1062
end: 1102
orientation: inline

LacI binding site
type: SequenceAnnotation
persistent identity: annotation2383991
display id: annotation2383991
version: 1
top level: BBa_K1399012
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1399012/annotation2383991/range2383991/1

https://synbiohub.org/public/igem/BBa_K1399012/annotation2383991/range2383991/1
type: Range
persistent identity: range2383991
display id: range2383991
version: 1
direction: direction/forward
top level: BBa_K1399012
start: 166
end: 200
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2384007
display id: annotation2384007
version: 1
top level: BBa_K1399012
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1399012/annotation2384007/range2384007/1

https://synbiohub.org/public/igem/BBa_K1399012/annotation2384007/range2384007/1
type: Range
persistent identity: range2384007
display id: range2384007
version: 1
direction: direction/reverse
top level: BBa_K1399012
start: 974
end: 1102
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation2383986
display id: annotation2383986
version: 1
top level: BBa_K1399012
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1399012/annotation2383986/range2383986/1

https://synbiohub.org/public/igem/BBa_K1399012/annotation2383986/range2383986/1
type: Range
persistent identity: range2383986
display id: range2383986
version: 1
direction: direction/forward
top level: BBa_K1399012
start: 1
end: 200
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2383995
display id: annotation2383995
version: 1
top level: BBa_K1399012
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1399012/annotation2383995/range2383995/1

https://synbiohub.org/public/igem/BBa_K1399012/annotation2383995/range2383995/1
type: Range
persistent identity: range2383995
display id: range2383995
version: 1
direction: direction/forward
top level: BBa_K1399012
start: 227
end: 229
orientation: inline

BBa_K1497021
type: ComponentDefinition
persistent identity: BBa K1497021
display id: BBa_K1497021
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Naringenin sensor (FdeR) with mKate as reporter
creator: Sascha Hein, Niklas Hummel, Sebastian Barthel, Thomas Dohmen
created: 2014-10-06T11:00:00Z
modified: 2015-05-08T01:10:45Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1877_
m user id: 0
owner id: 12466
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: We modified the naringenin biosensor from Siedler et al. by elimination of one EcoRI restriction site and by changing the native ribosomal binding site into the strong RBS Bba_B0034. Based on this reporter protein and 3 different fluorescence proteins, we designed 3 new biosensors for in vivo detection and determination of naringenin. The natural source of FdeR is Herbaspirillum seropedicae. You can use our reporter for measuring naringenin concentrations in your samples. Depending on which fluorophor you want to detect, you can use one of three biosensors: GFP fluorescence K1497020 mKate (DsRed variant) fluorescence K1497021 CFP fluorescence K1497022
mutable notes: d
mutable provenance: d
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_K1497019
component: BBa_K1055000
sequence annotation: BBa_K1497019
sequence annotation: BBa_K1055000
sequence: https://synbiohub.org/public/igem/BBa_K1497021_sequence/1

BBa_K1497019
type: Component
persistent identity: component2398954
display id: component2398954
version: 1
top level: BBa_K1497021
access: public
definition: https://synbiohub.org/public/igem/BBa_K1497019/1

BBa_K1055000
type: Component
persistent identity: component2398955
display id: component2398955
version: 1
top level: BBa_K1497021
access: public
definition: https://synbiohub.org/public/igem/BBa_K1055000/1

BBa_K1497019
type: SequenceAnnotation
persistent identity: annotation2398954
display id: annotation2398954
version: 1
top level: BBa_K1497021
location: https://synbiohub.org/public/igem/BBa_K1497021/annotation2398954/range2398954/1
component: BBa_K1497019

https://synbiohub.org/public/igem/BBa_K1497021/annotation2398954/range2398954/1
type: Range
persistent identity: range2398954
display id: range2398954
version: 1
direction: direction/reverse
top level: BBa_K1497021
start: 1
end: 1214
orientation: inline

BBa_K1055000
type: SequenceAnnotation
persistent identity: annotation2398955
display id: annotation2398955
version: 1
top level: BBa_K1497021
location: https://synbiohub.org/public/igem/BBa_K1497021/annotation2398955/range2398955/1
component: BBa_K1055000

https://synbiohub.org/public/igem/BBa_K1497021/annotation2398955/range2398955/1
type: Range
persistent identity: range2398955
display id: range2398955
version: 1
direction: direction/reverse
top level: BBa_K1497021
start: 1221
end: 1937
orientation: inline

BBa_K1926013
type: ComponentDefinition
persistent identity: BBa K1926013
display id: BBa_K1926013
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: The mCHERRY UNIT: mCherry flanked by Vox
creator: Xinyi Liu
created: 2016-10-08T11:00:00Z
modified: 2016-10-11T05:50:50Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2393_
m user id: 28998
owner id: 28998
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This part is the mCHERRY UNIT of cyclebow system including a degradation tag named DBOX, a fluorescent gene mCherry and an sv40 terminator flanked by homodromous recognition site of recombinase VIKA named Vox. The whole part can be cut off by VIKA. You may use this part to: 1) Provide fluorescent red which can be quickly cut off by transient transfecting recombinase gene into nucleus; 2) Use it as a part of the flp-out system[5]. 5. Voutev, R. and E.J. Hubbard, A "FLP-Out" system for controlled gene expression in Caenorhabditis elegans. Genetics, 2008. 180(1): p. 103-19.
mutable notes: None
mutable provenance: The sequence of Vox and DBOX was retrieved from Addgene and got through denovo synthesis. mCHERRY and sv40 terminator were got from commercial plasmid: pentry dcas9 mcherry bira and pentry nls GFP/pcdna3.1, respectfully.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: mutate C into A to remove Pst1
sequence annotation: sv40 terminator
sequence annotation: vox
sequence annotation: mCherry
sequence annotation: TAA
sequence: https://synbiohub.org/public/igem/BBa_K1926013_sequence/1

mutate C into A to remove Pst1
type: SequenceAnnotation
persistent identity: annotation2491882
display id: annotation2491882
version: 1
top level: BBa_K1926013
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K1926013/annotation2491882/range2491882/1

https://synbiohub.org/public/igem/BBa_K1926013/annotation2491882/range2491882/1
type: Range
persistent identity: range2491882
display id: range2491882
version: 1
direction: direction/forward
top level: BBa_K1926013
start: 384
end: 384
orientation: inline

sv40 terminator
type: SequenceAnnotation
persistent identity: annotation2491888
display id: annotation2491888
version: 1
top level: BBa_K1926013
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1926013/annotation2491888/range2491888/1

https://synbiohub.org/public/igem/BBa_K1926013/annotation2491888/range2491888/1
type: Range
persistent identity: range2491888
display id: range2491888
version: 1
direction: direction/forward
top level: BBa_K1926013
start: 740
end: 882
orientation: inline

vox
type: SequenceAnnotation
persistent identity: annotation2491874
display id: annotation2491874
version: 1
top level: BBa_K1926013
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1926013/annotation2491874/range2491874/1

https://synbiohub.org/public/igem/BBa_K1926013/annotation2491874/range2491874/1
type: Range
persistent identity: range2491874
display id: range2491874
version: 1
direction: direction/forward
top level: BBa_K1926013
start: 1
end: 34
orientation: inline

mCherry
type: SequenceAnnotation
persistent identity: annotation2491876
display id: annotation2491876
version: 1
top level: BBa_K1926013
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1926013/annotation2491876/range2491876/1

https://synbiohub.org/public/igem/BBa_K1926013/annotation2491876/range2491876/1
type: Range
persistent identity: range2491876
display id: range2491876
version: 1
direction: direction/forward
top level: BBa_K1926013
start: 35
end: 739
orientation: inline

vox
type: SequenceAnnotation
persistent identity: annotation2491890
display id: annotation2491890
version: 1
top level: BBa_K1926013
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1926013/annotation2491890/range2491890/1

https://synbiohub.org/public/igem/BBa_K1926013/annotation2491890/range2491890/1
type: Range
persistent identity: range2491890
display id: range2491890
version: 1
direction: direction/forward
top level: BBa_K1926013
start: 883
end: 916
orientation: inline

TAA
type: SequenceAnnotation
persistent identity: annotation2491891
display id: annotation2491891
version: 1
top level: BBa_K1926013
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1926013/annotation2491891/range2491891/1

https://synbiohub.org/public/igem/BBa_K1926013/annotation2491891/range2491891/1
type: Range
persistent identity: range2491891
display id: range2491891
version: 1
direction: direction/forward
top level: BBa_K1926013
start: 759
end: 761
orientation: inline

BBa_K1926031
type: ComponentDefinition
persistent identity: BBa K1926031
display id: BBa_K1926031
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: The SNAP-mCHERRY- CYAN UNIT
creator: Xinyi Liu
created: 2016-10-09T11:00:00Z
modified: 2016-10-11T07:02:32Z
discontinued: false
dominant: false
group u list: _2393_
m user id: 28998
owner id: 28998
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This part is the SNAP-mCHERRY-CYAN UNIT of cyclebow including a SNAP UNIT, a mCHERRY UNIT and a plain CYAN. The SNAP UNIT, which can be cut off by CRE, includes an sv40 nuclear location sequence (NLS), a covalent labeling of fusion proteins named SNAP-tag?? and an sv40 terminator flanked by homodromous recognition site of recombinase CRE named loxP. The mCHERRY UNIT, which can be cut off by VIKA, includes a degradation tag named DBOX, a fluorescent gene mCherry and an sv40 terminator flanked by homodromous recognition site of recombinase VIKA named Vox. You may use this part to: 1) Test the cut-off efficiency of recombinase CRE and VIKA; 2) Estimate the time needed for genome repair because mCherry can only be expressed after the cut off of SNAP UNIT and genome repair; 3) Fast cut off this part by transient transfecting recombinase gene into nucleus; 4) When there is no CRE, you can use this part as the SNAP UNIT, which can help localize proteins in vivo with BLOCK and dye from NEB company.
mutable notes: None
mutable provenance: The SNAP-tag?? was brought from NEB company. NLS and sv40 terminator were got from commercial plasmid: pentry nls GFP/pcdna3.1. The sequence of Vox and DBOX was retrieved from Addgene and got through denovo synthesis. mCHERRY was got from commercial plasmid: pentry dcas9 mcherry bira. CYAN was got from commercial plasmid: puc18 cfp-yfp.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: BBa_K1926022
sequence annotation: CYAN
sequence annotation: mutate C into A to remove Xba1
sequence: https://synbiohub.org/public/igem/BBa_K1926031_sequence/1

BBa_K1926022
type: SequenceAnnotation
persistent identity: annotation2489581
display id: annotation2489581
version: 1
top level: BBa_K1926031
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1926031/annotation2489581/range2489581/1

https://synbiohub.org/public/igem/BBa_K1926031/annotation2489581/range2489581/1
type: Range
persistent identity: range2489581
display id: range2489581
version: 1
direction: direction/forward
top level: BBa_K1926031
start: 1
end: 1704
orientation: inline

CYAN
type: SequenceAnnotation
persistent identity: annotation2491946
display id: annotation2491946
version: 1
top level: BBa_K1926031
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1926031/annotation2491946/range2491946/1

https://synbiohub.org/public/igem/BBa_K1926031/annotation2491946/range2491946/1
type: Range
persistent identity: range2491946
display id: range2491946
version: 1
direction: direction/forward
top level: BBa_K1926031
start: 1712
end: 2428
orientation: inline

mutate C into A to remove Xba1
type: SequenceAnnotation
persistent identity: annotation2491947
display id: annotation2491947
version: 1
top level: BBa_K1926031
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K1926031/annotation2491947/range2491947/1

https://synbiohub.org/public/igem/BBa_K1926031/annotation2491947/range2491947/1
type: Range
persistent identity: range2491947
display id: range2491947
version: 1
direction: direction/forward
top level: BBa_K1926031
start: 1707
end: 1707
orientation: inline

BBa_K1673103
type: ComponentDefinition
persistent identity: BBa K1673103
display id: BBa_K1673103
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: UV Mutation Maximizied RFP
creator: Jarrod Shilts
created: 2015-09-16T11:00:00Z
modified: 2016-01-21T04:03:48Z
discontinued: false
dominant: false
group u list: _2091_
m user id: 4206
owner id: 19415
owning group id: 9
status: status/Planning
bookmark: false
mutable description: RFP sequence, based on E1010, with the number of possible pyrimidine dimer-forming sites increased to the maximum possible amount.
mutable notes: Sequence produced by the Vanderbilt iGEM 2015 mutation optimization software
mutable provenance: Synthesized DNA
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1673103_sequence/1

BBa_K1980009
type: ComponentDefinition
persistent identity: BBa K1980009
display id: BBa_K1980009
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pCusC CusR RFP
creator: Sam Garforth
created: 2016-10-10T11:00:00Z
modified: 2016-10-23T07:39:56Z
discontinued: false
dominant: false
group u list: _2447_
m user id: 29607
owner id: 29607
owning group id: 9
status: status/Planning
bookmark: false
mutable description: pCusC CusR RFP
mutable notes: Stuff.
mutable provenance: E. coli
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: -35
sequence annotation: RBS
sequence annotation: mKate2
sequence annotation: -10
sequence annotation: pCusC
sequence annotation: t to c mutation (val to Ala)
sequence annotation: CusR
sequence annotation: Hexahistidine
sequence: https://synbiohub.org/public/igem/BBa_K1980009_sequence/1

-35
type: SequenceAnnotation
persistent identity: annotation2525373
display id: annotation2525373
version: 1
top level: BBa_K1980009
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1980009/annotation2525373/range2525373/1

https://synbiohub.org/public/igem/BBa_K1980009/annotation2525373/range2525373/1
type: Range
persistent identity: range2525373
display id: range2525373
version: 1
direction: direction/forward
top level: BBa_K1980009
start: 60
end: 65
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2525372
display id: annotation2525372
version: 1
top level: BBa_K1980009
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1980009/annotation2525372/range2525372/1

https://synbiohub.org/public/igem/BBa_K1980009/annotation2525372/range2525372/1
type: Range
persistent identity: range2525372
display id: range2525372
version: 1
direction: direction/forward
top level: BBa_K1980009
start: 105
end: 116
orientation: inline

mKate2
type: SequenceAnnotation
persistent identity: annotation2525378
display id: annotation2525378
version: 1
top level: BBa_K1980009
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1980009/annotation2525378/range2525378/1

https://synbiohub.org/public/igem/BBa_K1980009/annotation2525378/range2525378/1
type: Range
persistent identity: range2525378
display id: range2525378
version: 1
direction: direction/forward
top level: BBa_K1980009
start: 838
end: 1530
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2525374
display id: annotation2525374
version: 1
top level: BBa_K1980009
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1980009/annotation2525374/range2525374/1

https://synbiohub.org/public/igem/BBa_K1980009/annotation2525374/range2525374/1
type: Range
persistent identity: range2525374
display id: range2525374
version: 1
direction: direction/forward
top level: BBa_K1980009
start: 81
end: 87
orientation: inline

pCusC
type: SequenceAnnotation
persistent identity: annotation2525371
display id: annotation2525371
version: 1
top level: BBa_K1980009
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1980009/annotation2525371/range2525371/1

https://synbiohub.org/public/igem/BBa_K1980009/annotation2525371/range2525371/1
type: Range
persistent identity: range2525371
display id: range2525371
version: 1
direction: direction/forward
top level: BBa_K1980009
start: 1
end: 98
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2525377
display id: annotation2525377
version: 1
top level: BBa_K1980009
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1980009/annotation2525377/range2525377/1

https://synbiohub.org/public/igem/BBa_K1980009/annotation2525377/range2525377/1
type: Range
persistent identity: range2525377
display id: range2525377
version: 1
direction: direction/forward
top level: BBa_K1980009
start: 822
end: 827
orientation: inline

t to c mutation (val to Ala)
type: SequenceAnnotation
persistent identity: annotation2525368
display id: annotation2525368
version: 1
top level: BBa_K1980009
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K1980009/annotation2525368/range2525368/1

https://synbiohub.org/public/igem/BBa_K1980009/annotation2525368/range2525368/1
type: Range
persistent identity: range2525368
display id: range2525368
version: 1
direction: direction/forward
top level: BBa_K1980009
start: 706
end: 706
orientation: inline

CusR
type: SequenceAnnotation
persistent identity: annotation2525375
display id: annotation2525375
version: 1
top level: BBa_K1980009
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1980009/annotation2525375/range2525375/1

https://synbiohub.org/public/igem/BBa_K1980009/annotation2525375/range2525375/1
type: Range
persistent identity: range2525375
display id: range2525375
version: 1
direction: direction/forward
top level: BBa_K1980009
start: 123
end: 809
orientation: inline

Hexahistidine
type: SequenceAnnotation
persistent identity: annotation2525379
display id: annotation2525379
version: 1
top level: BBa_K1980009
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1980009/annotation2525379/range2525379/1

https://synbiohub.org/public/igem/BBa_K1980009/annotation2525379/range2525379/1
type: Range
persistent identity: range2525379
display id: range2525379
version: 1
direction: direction/forward
top level: BBa_K1980009
start: 1531
end: 1554
orientation: inline

mCherryC
type: ComponentDefinition
persistent identity: BBa K1093017
display id: BBa_K1093017
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: mCherry C-terminal
creator: Anna Miścicka
created: 2013-09-20T11:00:00Z
modified: 2016-01-26T03:07:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1403_
m user id: 4206
owner id: 18431
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This is N-terminal of mCherry, that can be used in Bimolecular Fluorescence Complementation system (BiFC).
mutable notes: Created by PCR on mCherry.
mutable provenance: PartsRegistry J06505, originally from Discosoma sp.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: stop
sequence annotation: mCherry C-terminal
sequence: https://synbiohub.org/public/igem/BBa_K1093017_sequence/1

stop
type: SequenceAnnotation
persistent identity: annotation2357376
display id: annotation2357376
version: 1
top level: mCherryC
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1093017/annotation2357376/range2357376/1

https://synbiohub.org/public/igem/BBa_K1093017/annotation2357376/range2357376/1
type: Range
persistent identity: range2357376
display id: range2357376
version: 1
direction: direction/forward
top level: mCherryC
start: 240
end: 243
orientation: inline

mCherry C-terminal
type: SequenceAnnotation
persistent identity: annotation2357377
display id: annotation2357377
version: 1
top level: mCherryC
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1093017/annotation2357377/range2357377/1

https://synbiohub.org/public/igem/BBa_K1093017/annotation2357377/range2357377/1
type: Range
persistent identity: range2357377
display id: range2357377
version: 1
direction: direction/forward
top level: mCherryC
start: 1
end: 239
orientation: inline

dTomato
type: ComponentDefinition
persistent identity: BBa K1688004
display id: BBa_K1688004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: dTomato (inc RBS)
creator: Erik Gullberg
created: 2015-09-14T11:00:00Z
modified: 2015-09-18T10:34:03Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2106_
m user id: 27118
owner id: 27121
owning group id: 9
status: status/Planning
bookmark: false
mutable description: The dTomato protein is a fluorescent dimer protein that emits orange-red light when it is excited by green-yellow light. It is preferable to use ??? especially in self-made fluorometry tests ??? because the excitation wavelengths and the emission wavelengths don't overlap as much as in other fluorescent proteins. The dTomato excitation peak is at 554 nm and 50% of it is at 510 nm. Also, its emission peak is at 581 nm and its 50% emission at 629 nm (Figure 1). Characterization has been made with the Anderson promoters BBa_J23101 and (construct BBa_K1688010) and BBa_J23110 (construct BBa_K1688011).
mutable provenance: It was derived from a monomeric mutant of the anthozoan coral RFP, DsRed
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_B0034
component: BBa_K1688019
sequence annotation: BBa_K1688019
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K1688004_sequence/1

BBa_B0034
type: Component
persistent identity: component2457404
display id: component2457404
version: 1
top level: dTomato
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K1688019
type: Component
persistent identity: component2457405
display id: component2457405
version: 1
top level: dTomato
access: public
definition: dTomato

BBa_K1688019
type: SequenceAnnotation
persistent identity: annotation2457405
display id: annotation2457405
version: 1
top level: dTomato
location: https://synbiohub.org/public/igem/BBa_K1688004/annotation2457405/range2457405/1
component: BBa_K1688019

https://synbiohub.org/public/igem/BBa_K1688004/annotation2457405/range2457405/1
type: Range
persistent identity: range2457405
display id: range2457405
version: 1
direction: direction/reverse
top level: dTomato
start: 19
end: 726
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2457404
display id: annotation2457404
version: 1
top level: dTomato
location: https://synbiohub.org/public/igem/BBa_K1688004/annotation2457404/range2457404/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1688004/annotation2457404/range2457404/1
type: Range
persistent identity: range2457404
display id: range2457404
version: 1
direction: direction/reverse
top level: dTomato
start: 1
end: 12
orientation: inline

RFP2
type: ComponentDefinition
persistent identity: BBa I715023
display id: BBa_I715023
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Carboxyl portion of RFP
creator: Andrew Martens
created: 2007-06-24T11:00:00Z
modified: 2016-01-25T04:44:49Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _120_
m user id: 4206
owner id: 201
owning group id: 61
sample status: In stock
status: status/Available
bookmark: false
mutable description: The second portion of the RFP gene, after the HixC insertion point.
mutable notes: Used web tool.
mutable provenance: Sequence from part BBa_E1010.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: extra base (avoid frameshift)
sequence annotation: Continue coding sequence
sequence annotation: (two in tandem)
sequence: https://synbiohub.org/public/igem/BBa_I715023_sequence/1

extra base (avoid frameshift)
type: SequenceAnnotation
persistent identity: annotation1935382
display id: annotation1935382
version: 1
top level: RFP2
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I715023/annotation1935382/range1935382/1

https://synbiohub.org/public/igem/BBa_I715023/annotation1935382/range1935382/1
type: Range
persistent identity: range1935382
display id: range1935382
version: 1
direction: direction/forward
top level: RFP2
start: 1
end: 1
orientation: inline

Continue coding sequence
type: SequenceAnnotation
persistent identity: annotation1935383
display id: annotation1935383
version: 1
top level: RFP2
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_I715023/annotation1935383/range1935383/1

https://synbiohub.org/public/igem/BBa_I715023/annotation1935383/range1935383/1
type: Range
persistent identity: range1935383
display id: range1935383
version: 1
direction: direction/forward
top level: RFP2
start: 2
end: 214
orientation: inline

(two in tandem)
type: SequenceAnnotation
persistent identity: annotation1935384
display id: annotation1935384
version: 1
top level: RFP2
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_I715023/annotation1935384/range1935384/1

https://synbiohub.org/public/igem/BBa_I715023/annotation1935384/range1935384/1
type: Range
persistent identity: range1935384
display id: range1935384
version: 1
direction: direction/forward
top level: RFP2
start: 215
end: 219
orientation: inline

BBa_K1399011
type: ComponentDefinition
persistent identity: BBa K1399011
display id: BBa_K1399011
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Plac-RFP(LVA)
creator: Anna Stikane
created: 2014-09-18T11:00:00Z
modified: 2015-05-08T01:10:16Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1777_
m user id: 0
owner id: 22477
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: Lactose/IPTG inducible promoter with RBS, RFP reporter tagged with LVA-ssrA degradation tag and terminator. The tagged RFP is actively degraded within cell, thus provides better temporal resolution of red fluorescence and promoter activity. This part can be considered as improved version of BBa_J04451 as it does not contain StuI restriction site coding for additional 'RP' residues between RFP and LVA tag.
mutable notes: Part was assembled using BrickClip assembly (BBF RFC 104) using other biobrick parts as templates. BrickClip assembly is a special case of more general Paperclip assembly method.[1]
mutable provenance: other biobricks
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: end of LacI coding region (inactive)
sequence annotation: T7 TE
sequence annotation: Barcode of BBa_E1010
sequence annotation: LacI binding site
sequence annotation: conserved
sequence annotation: polya
sequence annotation: BBa_B0015
sequence annotation: BBa_R0010
sequence annotation: BBa_B0010
sequence annotation: LVA-ssrA tag
sequence annotation: BBa_E1010 w/o stop
sequence annotation: stop
sequence annotation: -10
sequence annotation: CAP binding site
sequence annotation: BBa_K1399001
sequence annotation: stem_loop
sequence annotation: BBa_B0012
sequence annotation: -35
sequence annotation: start
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K1399011_sequence/1

end of LacI coding region (inactive)
type: SequenceAnnotation
persistent identity: annotation2386290
display id: annotation2386290
version: 1
top level: BBa_K1399011
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1399011/annotation2386290/range2386290/1

https://synbiohub.org/public/igem/BBa_K1399011/annotation2386290/range2386290/1
type: Range
persistent identity: range2386290
display id: range2386290
version: 1
direction: direction/forward
top level: BBa_K1399011
start: 1
end: 88
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation2386309
display id: annotation2386309
version: 1
top level: BBa_K1399011
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1399011/annotation2386309/range2386309/1

https://synbiohub.org/public/igem/BBa_K1399011/annotation2386309/range2386309/1
type: Range
persistent identity: range2386309
display id: range2386309
version: 1
direction: direction/forward
top level: BBa_K1399011
start: 1069
end: 1088
orientation: inline

Barcode of BBa_E1010
type: SequenceAnnotation
persistent identity: annotation2386304
display id: annotation2386304
version: 1
top level: BBa_K1399011
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1399011/annotation2386304/range2386304/1

https://synbiohub.org/public/igem/BBa_K1399011/annotation2386304/range2386304/1
type: Range
persistent identity: range2386304
display id: range2386304
version: 1
direction: direction/forward
top level: BBa_K1399011
start: 941
end: 965
orientation: inline

LacI binding site
type: SequenceAnnotation
persistent identity: annotation2386294
display id: annotation2386294
version: 1
top level: BBa_K1399011
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1399011/annotation2386294/range2386294/1

https://synbiohub.org/public/igem/BBa_K1399011/annotation2386294/range2386294/1
type: Range
persistent identity: range2386294
display id: range2386294
version: 1
direction: direction/forward
top level: BBa_K1399011
start: 166
end: 200
orientation: inline

conserved
type: SequenceAnnotation
persistent identity: annotation2386297
display id: annotation2386297
version: 1
top level: BBa_K1399011
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K1399011/annotation2386297/range2386297/1

https://synbiohub.org/public/igem/BBa_K1399011/annotation2386297/range2386297/1
type: Range
persistent identity: range2386297
display id: range2386297
version: 1
direction: direction/forward
top level: BBa_K1399011
start: 213
end: 216
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation2386310
display id: annotation2386310
version: 1
top level: BBa_K1399011
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_K1399011/annotation2386310/range2386310/1

https://synbiohub.org/public/igem/BBa_K1399011/annotation2386310/range2386310/1
type: Range
persistent identity: range2386310
display id: range2386310
version: 1
direction: direction/forward
top level: BBa_K1399011
start: 1089
end: 1102
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2386305
display id: annotation2386305
version: 1
top level: BBa_K1399011
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1399011/annotation2386305/range2386305/1

https://synbiohub.org/public/igem/BBa_K1399011/annotation2386305/range2386305/1
type: Range
persistent identity: range2386305
display id: range2386305
version: 1
direction: direction/reverse
top level: BBa_K1399011
start: 974
end: 1102
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation2386289
display id: annotation2386289
version: 1
top level: BBa_K1399011
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1399011/annotation2386289/range2386289/1

https://synbiohub.org/public/igem/BBa_K1399011/annotation2386289/range2386289/1
type: Range
persistent identity: range2386289
display id: range2386289
version: 1
direction: direction/forward
top level: BBa_K1399011
start: 1
end: 200
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2386306
display id: annotation2386306
version: 1
top level: BBa_K1399011
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1399011/annotation2386306/range2386306/1

https://synbiohub.org/public/igem/BBa_K1399011/annotation2386306/range2386306/1
type: Range
persistent identity: range2386306
display id: range2386306
version: 1
direction: direction/forward
top level: BBa_K1399011
start: 974
end: 1053
orientation: inline

LVA-ssrA tag
type: SequenceAnnotation
persistent identity: annotation2386301
display id: annotation2386301
version: 1
top level: BBa_K1399011
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1399011/annotation2386301/range2386301/1

https://synbiohub.org/public/igem/BBa_K1399011/annotation2386301/range2386301/1
type: Range
persistent identity: range2386301
display id: range2386301
version: 1
direction: direction/forward
top level: BBa_K1399011
start: 902
end: 934
orientation: inline

BBa_E1010 w/o stop
type: SequenceAnnotation
persistent identity: annotation2386300
display id: annotation2386300
version: 1
top level: BBa_K1399011
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1399011/annotation2386300/range2386300/1

https://synbiohub.org/public/igem/BBa_K1399011/annotation2386300/range2386300/1
type: Range
persistent identity: range2386300
display id: range2386300
version: 1
direction: direction/forward
top level: BBa_K1399011
start: 230
end: 901
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2386302
display id: annotation2386302
version: 1
top level: BBa_K1399011
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1399011/annotation2386302/range2386302/1

https://synbiohub.org/public/igem/BBa_K1399011/annotation2386302/range2386302/1
type: Range
persistent identity: range2386302
display id: range2386302
version: 1
direction: direction/forward
top level: BBa_K1399011
start: 935
end: 937
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2386293
display id: annotation2386293
version: 1
top level: BBa_K1399011
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1399011/annotation2386293/range2386293/1

https://synbiohub.org/public/igem/BBa_K1399011/annotation2386293/range2386293/1
type: Range
persistent identity: range2386293
display id: range2386293
version: 1
direction: direction/forward
top level: BBa_K1399011
start: 161
end: 166
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2386303
display id: annotation2386303
version: 1
top level: BBa_K1399011
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1399011/annotation2386303/range2386303/1

https://synbiohub.org/public/igem/BBa_K1399011/annotation2386303/range2386303/1
type: Range
persistent identity: range2386303
display id: range2386303
version: 1
direction: direction/forward
top level: BBa_K1399011
start: 938
end: 940
orientation: inline

CAP binding site
type: SequenceAnnotation
persistent identity: annotation2386291
display id: annotation2386291
version: 1
top level: BBa_K1399011
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1399011/annotation2386291/range2386291/1

https://synbiohub.org/public/igem/BBa_K1399011/annotation2386291/range2386291/1
type: Range
persistent identity: range2386291
display id: range2386291
version: 1
direction: direction/forward
top level: BBa_K1399011
start: 89
end: 126
orientation: inline

BBa_K1399001
type: SequenceAnnotation
persistent identity: annotation2386299
display id: annotation2386299
version: 1
top level: BBa_K1399011
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1399011/annotation2386299/range2386299/1

https://synbiohub.org/public/igem/BBa_K1399011/annotation2386299/range2386299/1
type: Range
persistent identity: range2386299
display id: range2386299
version: 1
direction: direction/reverse
top level: BBa_K1399011
start: 227
end: 940
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation2386307
display id: annotation2386307
version: 1
top level: BBa_K1399011
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1399011/annotation2386307/range2386307/1

https://synbiohub.org/public/igem/BBa_K1399011/annotation2386307/range2386307/1
type: Range
persistent identity: range2386307
display id: range2386307
version: 1
direction: direction/forward
top level: BBa_K1399011
start: 985
end: 1028
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2386308
display id: annotation2386308
version: 1
top level: BBa_K1399011
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1399011/annotation2386308/range2386308/1

https://synbiohub.org/public/igem/BBa_K1399011/annotation2386308/range2386308/1
type: Range
persistent identity: range2386308
display id: range2386308
version: 1
direction: direction/forward
top level: BBa_K1399011
start: 1062
end: 1102
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2386311
display id: annotation2386311
version: 1
top level: BBa_K1399011
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1399011/annotation2386311/range2386311/1

https://synbiohub.org/public/igem/BBa_K1399011/annotation2386311/range2386311/1
type: Range
persistent identity: range2386311
display id: range2386311
version: 1
direction: direction/forward
top level: BBa_K1399011
start: 1095
end: 1095
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2386292
display id: annotation2386292
version: 1
top level: BBa_K1399011
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1399011/annotation2386292/range2386292/1

https://synbiohub.org/public/igem/BBa_K1399011/annotation2386292/range2386292/1
type: Range
persistent identity: range2386292
display id: range2386292
version: 1
direction: direction/forward
top level: BBa_K1399011
start: 137
end: 142
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2386295
display id: annotation2386295
version: 1
top level: BBa_K1399011
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1399011/annotation2386295/range2386295/1

https://synbiohub.org/public/igem/BBa_K1399011/annotation2386295/range2386295/1
type: Range
persistent identity: range2386295
display id: range2386295
version: 1
direction: direction/forward
top level: BBa_K1399011
start: 173
end: 173
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2386298
display id: annotation2386298
version: 1
top level: BBa_K1399011
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1399011/annotation2386298/range2386298/1

https://synbiohub.org/public/igem/BBa_K1399011/annotation2386298/range2386298/1
type: Range
persistent identity: range2386298
display id: range2386298
version: 1
direction: direction/forward
top level: BBa_K1399011
start: 227
end: 229
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2386296
display id: annotation2386296
version: 1
top level: BBa_K1399011
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1399011/annotation2386296/range2386296/1

https://synbiohub.org/public/igem/BBa_K1399011/annotation2386296/range2386296/1
type: Range
persistent identity: range2386296
display id: range2386296
version: 1
direction: direction/reverse
top level: BBa_K1399011
start: 209
end: 220
orientation: inline

BBa_K1184000
type: ComponentDefinition
persistent identity: BBa K1184000
display id: BBa_K1184000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: KillerRed
creator: Eric Pederson
created: 2013-09-03T11:00:00Z
modified: 2016-01-25T02:40:37Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1497_
m user id: 4206
owner id: 12713
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: KillerRed is a red fluorescent protein that produces reactive oxygen species (ROS) in the presence of yellow-orange light (540-585 nm). KillerRed is engineered from Aequorea victoria GFP (avGFP) to be phototoxic. It has been shown that KillerRed produces superoxide radical anions by reacting with water. Superoxide reacts with the chromophore of KillerRed, causing it to become dark, which ultimately gives rise to a bleaching effect. KillerRed is spectrally similar to mRFP1 with a similar brightness. KillerRed is oligomeric and may form large aggregates. This sequence is codon optimized for mammalian cells and has 14 rare proline codons for E. coli (CCC) and one rare arginine codon (AGA). Codon optimization should be taken into consideration if large amounts of the protein are required. Expression on a high-copy plasmid has produced detectable fluorescent signal, however.
mutable notes: This sequence is not codon optimized for E. coli.
mutable provenance: Engineered from avGFP. Provided by the Bruchez lab at Carnegie Mellon
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Phototoxic Mutation Asn(147)
sequence annotation: Phototoxic Mutation Ser(121)
sequence annotation: Phototoxic Mutation Glu(70)
sequence annotation: Chromophore
sequence annotation: Double Stop
sequence annotation: Start
sequence annotation: cds
sequence annotation: Phototoxic Mutation Ala(163)
sequence: https://synbiohub.org/public/igem/BBa_K1184000_sequence/1

Phototoxic Mutation Asn(147)
type: SequenceAnnotation
persistent identity: annotation2334960
display id: annotation2334960
version: 1
top level: BBa_K1184000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1184000/annotation2334960/range2334960/1

https://synbiohub.org/public/igem/BBa_K1184000/annotation2334960/range2334960/1
type: Range
persistent identity: range2334960
display id: range2334960
version: 1
direction: direction/forward
top level: BBa_K1184000
start: 439
end: 441
orientation: inline

Phototoxic Mutation Ser(121)
type: SequenceAnnotation
persistent identity: annotation2334957
display id: annotation2334957
version: 1
top level: BBa_K1184000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1184000/annotation2334957/range2334957/1

https://synbiohub.org/public/igem/BBa_K1184000/annotation2334957/range2334957/1
type: Range
persistent identity: range2334957
display id: range2334957
version: 1
direction: direction/forward
top level: BBa_K1184000
start: 367
end: 369
orientation: inline

Phototoxic Mutation Glu(70)
type: SequenceAnnotation
persistent identity: annotation2334956
display id: annotation2334956
version: 1
top level: BBa_K1184000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1184000/annotation2334956/range2334956/1

https://synbiohub.org/public/igem/BBa_K1184000/annotation2334956/range2334956/1
type: Range
persistent identity: range2334956
display id: range2334956
version: 1
direction: direction/forward
top level: BBa_K1184000
start: 208
end: 210
orientation: inline

Chromophore
type: SequenceAnnotation
persistent identity: annotation2334958
display id: annotation2334958
version: 1
top level: BBa_K1184000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1184000/annotation2334958/range2334958/1

https://synbiohub.org/public/igem/BBa_K1184000/annotation2334958/range2334958/1
type: Range
persistent identity: range2334958
display id: range2334958
version: 1
direction: direction/forward
top level: BBa_K1184000
start: 199
end: 207
orientation: inline

Double Stop
type: SequenceAnnotation
persistent identity: annotation2334953
display id: annotation2334953
version: 1
top level: BBa_K1184000
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1184000/annotation2334953/range2334953/1

https://synbiohub.org/public/igem/BBa_K1184000/annotation2334953/range2334953/1
type: Range
persistent identity: range2334953
display id: range2334953
version: 1
direction: direction/forward
top level: BBa_K1184000
start: 718
end: 723
orientation: inline

Start
type: SequenceAnnotation
persistent identity: annotation2334954
display id: annotation2334954
version: 1
top level: BBa_K1184000
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1184000/annotation2334954/range2334954/1

https://synbiohub.org/public/igem/BBa_K1184000/annotation2334954/range2334954/1
type: Range
persistent identity: range2334954
display id: range2334954
version: 1
direction: direction/forward
top level: BBa_K1184000
start: 1
end: 3
orientation: inline

cds
type: SequenceAnnotation
persistent identity: annotation2334955
display id: annotation2334955
version: 1
top level: BBa_K1184000
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1184000/annotation2334955/range2334955/1

https://synbiohub.org/public/igem/BBa_K1184000/annotation2334955/range2334955/1
type: Range
persistent identity: range2334955
display id: range2334955
version: 1
direction: direction/forward
top level: BBa_K1184000
start: 1
end: 717
orientation: inline

Phototoxic Mutation Ala(163)
type: SequenceAnnotation
persistent identity: annotation2334965
display id: annotation2334965
version: 1
top level: BBa_K1184000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1184000/annotation2334965/range2334965/1

https://synbiohub.org/public/igem/BBa_K1184000/annotation2334965/range2334965/1
type: Range
persistent identity: range2334965
display id: range2334965
version: 1
direction: direction/forward
top level: BBa_K1184000
start: 487
end: 489
orientation: inline

BBa_K1399010
type: ComponentDefinition
persistent identity: BBa K1399010
display id: BBa_K1399010
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Plac-RFP(AAV)
creator: Anna Stikane
created: 2014-09-18T11:00:00Z
modified: 2015-05-08T01:10:16Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1777_
m user id: 0
owner id: 22477
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: Lactose/IPTG inducible promoter wuth RFP reporter tagged with AAV-ssrA degradation tag followed by terminator. The tagged RFP is actively degraded within cell, thus provides better temporal resolution of red fluorescence and promoter activity. Part was assembled using BrickClip assembly (BBF RFC 104) using other biobrick parts as templates. BrickClip assembly is a special case of more general Paperclip assembly method.[1] Reference [1] Trubitsyna, M., Michlewski, G., Cai, Y., Elfick, A. & French, C. E. PaperClip: rapid multi-part DNA assembly from existing libraries. Nucleic Acids Res. 1???8 (2014). doi:10.1093/nar/gku829
mutable notes: no
mutable provenance: Other biobrick parts
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: T7 TE
sequence annotation: BBa_B0034
sequence annotation: stop
sequence annotation: BBa_K1399000
sequence annotation: CAP binding site
sequence annotation: stem_loop
sequence annotation: start
sequence annotation: BBa_B0010
sequence annotation: conserved
sequence annotation: BBa_B0012
sequence annotation: polya
sequence annotation: BBa_E1010 w/o stop
sequence annotation: end of LacI coding region (inactive)
sequence annotation: BBa_R0010
sequence annotation: barcode from BBa_E1010
sequence annotation: BBa_B0015
sequence annotation: -35
sequence annotation: AAV-ssrA tag
sequence annotation: LacI binding site
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_K1399010_sequence/1

T7 TE
type: SequenceAnnotation
persistent identity: annotation2383951
display id: annotation2383951
version: 1
top level: BBa_K1399010
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1399010/annotation2383951/range2383951/1

https://synbiohub.org/public/igem/BBa_K1399010/annotation2383951/range2383951/1
type: Range
persistent identity: range2383951
display id: range2383951
version: 1
direction: direction/forward
top level: BBa_K1399010
start: 1069
end: 1088
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2383941
display id: annotation2383941
version: 1
top level: BBa_K1399010
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1399010/annotation2383941/range2383941/1

https://synbiohub.org/public/igem/BBa_K1399010/annotation2383941/range2383941/1
type: Range
persistent identity: range2383941
display id: range2383941
version: 1
direction: direction/reverse
top level: BBa_K1399010
start: 209
end: 220
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2383945
display id: annotation2383945
version: 1
top level: BBa_K1399010
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1399010/annotation2383945/range2383945/1

https://synbiohub.org/public/igem/BBa_K1399010/annotation2383945/range2383945/1
type: Range
persistent identity: range2383945
display id: range2383945
version: 1
direction: direction/forward
top level: BBa_K1399010
start: 935
end: 937
orientation: inline

BBa_K1399000
type: SequenceAnnotation
persistent identity: annotation2383947
display id: annotation2383947
version: 1
top level: BBa_K1399010
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1399010/annotation2383947/range2383947/1

https://synbiohub.org/public/igem/BBa_K1399010/annotation2383947/range2383947/1
type: Range
persistent identity: range2383947
display id: range2383947
version: 1
direction: direction/reverse
top level: BBa_K1399010
start: 227
end: 940
orientation: inline

CAP binding site
type: SequenceAnnotation
persistent identity: annotation2383935
display id: annotation2383935
version: 1
top level: BBa_K1399010
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1399010/annotation2383935/range2383935/1

https://synbiohub.org/public/igem/BBa_K1399010/annotation2383935/range2383935/1
type: Range
persistent identity: range2383935
display id: range2383935
version: 1
direction: direction/forward
top level: BBa_K1399010
start: 89
end: 126
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation2383949
display id: annotation2383949
version: 1
top level: BBa_K1399010
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1399010/annotation2383949/range2383949/1

https://synbiohub.org/public/igem/BBa_K1399010/annotation2383949/range2383949/1
type: Range
persistent identity: range2383949
display id: range2383949
version: 1
direction: direction/forward
top level: BBa_K1399010
start: 985
end: 1028
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2383942
display id: annotation2383942
version: 1
top level: BBa_K1399010
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1399010/annotation2383942/range2383942/1

https://synbiohub.org/public/igem/BBa_K1399010/annotation2383942/range2383942/1
type: Range
persistent identity: range2383942
display id: range2383942
version: 1
direction: direction/forward
top level: BBa_K1399010
start: 227
end: 229
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2383948
display id: annotation2383948
version: 1
top level: BBa_K1399010
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1399010/annotation2383948/range2383948/1

https://synbiohub.org/public/igem/BBa_K1399010/annotation2383948/range2383948/1
type: Range
persistent identity: range2383948
display id: range2383948
version: 1
direction: direction/forward
top level: BBa_K1399010
start: 974
end: 1053
orientation: inline

conserved
type: SequenceAnnotation
persistent identity: annotation2383940
display id: annotation2383940
version: 1
top level: BBa_K1399010
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K1399010/annotation2383940/range2383940/1

https://synbiohub.org/public/igem/BBa_K1399010/annotation2383940/range2383940/1
type: Range
persistent identity: range2383940
display id: range2383940
version: 1
direction: direction/forward
top level: BBa_K1399010
start: 213
end: 216
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2383939
display id: annotation2383939
version: 1
top level: BBa_K1399010
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1399010/annotation2383939/range2383939/1

https://synbiohub.org/public/igem/BBa_K1399010/annotation2383939/range2383939/1
type: Range
persistent identity: range2383939
display id: range2383939
version: 1
direction: direction/forward
top level: BBa_K1399010
start: 173
end: 173
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2383950
display id: annotation2383950
version: 1
top level: BBa_K1399010
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1399010/annotation2383950/range2383950/1

https://synbiohub.org/public/igem/BBa_K1399010/annotation2383950/range2383950/1
type: Range
persistent identity: range2383950
display id: range2383950
version: 1
direction: direction/forward
top level: BBa_K1399010
start: 1062
end: 1102
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation2383952
display id: annotation2383952
version: 1
top level: BBa_K1399010
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_K1399010/annotation2383952/range2383952/1

https://synbiohub.org/public/igem/BBa_K1399010/annotation2383952/range2383952/1
type: Range
persistent identity: range2383952
display id: range2383952
version: 1
direction: direction/forward
top level: BBa_K1399010
start: 1089
end: 1102
orientation: inline

BBa_E1010 w/o stop
type: SequenceAnnotation
persistent identity: annotation2383943
display id: annotation2383943
version: 1
top level: BBa_K1399010
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1399010/annotation2383943/range2383943/1

https://synbiohub.org/public/igem/BBa_K1399010/annotation2383943/range2383943/1
type: Range
persistent identity: range2383943
display id: range2383943
version: 1
direction: direction/forward
top level: BBa_K1399010
start: 230
end: 901
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2383946
display id: annotation2383946
version: 1
top level: BBa_K1399010
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1399010/annotation2383946/range2383946/1

https://synbiohub.org/public/igem/BBa_K1399010/annotation2383946/range2383946/1
type: Range
persistent identity: range2383946
display id: range2383946
version: 1
direction: direction/forward
top level: BBa_K1399010
start: 938
end: 940
orientation: inline

end of LacI coding region (inactive)
type: SequenceAnnotation
persistent identity: annotation2383934
display id: annotation2383934
version: 1
top level: BBa_K1399010
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1399010/annotation2383934/range2383934/1

https://synbiohub.org/public/igem/BBa_K1399010/annotation2383934/range2383934/1
type: Range
persistent identity: range2383934
display id: range2383934
version: 1
direction: direction/forward
top level: BBa_K1399010
start: 1
end: 88
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation2383933
display id: annotation2383933
version: 1
top level: BBa_K1399010
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1399010/annotation2383933/range2383933/1

https://synbiohub.org/public/igem/BBa_K1399010/annotation2383933/range2383933/1
type: Range
persistent identity: range2383933
display id: range2383933
version: 1
direction: direction/forward
top level: BBa_K1399010
start: 1
end: 200
orientation: inline

barcode from BBa_E1010
type: SequenceAnnotation
persistent identity: annotation2386257
display id: annotation2386257
version: 1
top level: BBa_K1399010
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1399010/annotation2386257/range2386257/1

https://synbiohub.org/public/igem/BBa_K1399010/annotation2386257/range2386257/1
type: Range
persistent identity: range2386257
display id: range2386257
version: 1
direction: direction/forward
top level: BBa_K1399010
start: 941
end: 965
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2383953
display id: annotation2383953
version: 1
top level: BBa_K1399010
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1399010/annotation2383953/range2383953/1

https://synbiohub.org/public/igem/BBa_K1399010/annotation2383953/range2383953/1
type: Range
persistent identity: range2383953
display id: range2383953
version: 1
direction: direction/forward
top level: BBa_K1399010
start: 1095
end: 1095
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2383954
display id: annotation2383954
version: 1
top level: BBa_K1399010
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1399010/annotation2383954/range2383954/1

https://synbiohub.org/public/igem/BBa_K1399010/annotation2383954/range2383954/1
type: Range
persistent identity: range2383954
display id: range2383954
version: 1
direction: direction/reverse
top level: BBa_K1399010
start: 974
end: 1102
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2383936
display id: annotation2383936
version: 1
top level: BBa_K1399010
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1399010/annotation2383936/range2383936/1

https://synbiohub.org/public/igem/BBa_K1399010/annotation2383936/range2383936/1
type: Range
persistent identity: range2383936
display id: range2383936
version: 1
direction: direction/forward
top level: BBa_K1399010
start: 137
end: 142
orientation: inline

AAV-ssrA tag
type: SequenceAnnotation
persistent identity: annotation2383944
display id: annotation2383944
version: 1
top level: BBa_K1399010
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1399010/annotation2383944/range2383944/1

https://synbiohub.org/public/igem/BBa_K1399010/annotation2383944/range2383944/1
type: Range
persistent identity: range2383944
display id: range2383944
version: 1
direction: direction/forward
top level: BBa_K1399010
start: 902
end: 934
orientation: inline

LacI binding site
type: SequenceAnnotation
persistent identity: annotation2383938
display id: annotation2383938
version: 1
top level: BBa_K1399010
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1399010/annotation2383938/range2383938/1

https://synbiohub.org/public/igem/BBa_K1399010/annotation2383938/range2383938/1
type: Range
persistent identity: range2383938
display id: range2383938
version: 1
direction: direction/forward
top level: BBa_K1399010
start: 166
end: 200
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2383937
display id: annotation2383937
version: 1
top level: BBa_K1399010
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1399010/annotation2383937/range2383937/1

https://synbiohub.org/public/igem/BBa_K1399010/annotation2383937/range2383937/1
type: Range
persistent identity: range2383937
display id: range2383937
version: 1
direction: direction/forward
top level: BBa_K1399010
start: 161
end: 166
orientation: inline

BBa_K106004
type: ComponentDefinition
persistent identity: BBa K106004
display id: BBa_K106004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: mCherry, Aar1 AB part
creator: Ryan Quan
created: 2008-10-21T11:00:00Z
modified: 2016-01-26T09:49:59Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _226_
m user id: 4206
owner id: 3421
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This is the coding sequence for mCherry with AB ends for Aar1 cloning.
mutable notes: Since this is an AB part, there is an ATG but no stop codon. For more information on AarI cloning, please refer to the UCSF 2008 igem page.
mutable provenance: Yeast genome
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K106004_sequence/1

mRFP1
type: ComponentDefinition
persistent identity: BBa E2050
display id: BBa_E2050
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: derivative of mRFP1, yeast-optimized
creator: ryu
created: 2005-01-24T12:00:00Z
modified: 2016-01-25T02:35:54Z
discontinued: false
dominant: false
experience: experience/None
group u list: _8_
m user id: 4206
owner id: 230
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: mRFP derivative. Ex548nm/Em562. yeast codon optimized.
mutable notes: Contains N- and C-terminal linker sequences (with homology to biobrick parts BBa_E2060 (mCherry), BBa_E2020 (Cerulean CFP), and BBa_E2030 (Venus YFP) to facilitate color swapping in yeast. Adds N-"MATSG" and "GSGTA"-C. to published amino acid sequence. Double TAATAA stop codon. Missing EcoRI, HindIII, NotI, NdeI, XhoI, RsrII, BamHI, NcoI, BglI, SpeI, XbaI, and PstI. except for 5' and 3' ends no significant sequence identity runs to mCherry (BBa_E2060).
mutable provenance: Shaner et al, 2004. Nat Biotech (22):1567-1571
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: GSGTA linker
sequence annotation: MATSG linker
sequence annotation: Help:Barcodes
sequence annotation: mOrange
sequence: https://synbiohub.org/public/igem/BBa_E2050_sequence/1

GSGTA linker
type: SequenceAnnotation
persistent identity: annotation1431919
display id: annotation1431919
version: 1
top level: mRFP1
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_E2050/annotation1431919/range1431919/1

https://synbiohub.org/public/igem/BBa_E2050/annotation1431919/range1431919/1
type: Range
persistent identity: range1431919
display id: range1431919
version: 1
direction: direction/forward
top level: mRFP1
start: 724
end: 738
orientation: inline

MATSG linker
type: SequenceAnnotation
persistent identity: annotation1431915
display id: annotation1431915
version: 1
top level: mRFP1
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_E2050/annotation1431915/range1431915/1

https://synbiohub.org/public/igem/BBa_E2050/annotation1431915/range1431915/1
type: Range
persistent identity: range1431915
display id: range1431915
version: 1
direction: direction/forward
top level: mRFP1
start: 1
end: 15
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2214024
display id: annotation2214024
version: 1
top level: mRFP1
role: SO:0000807
role: feature/barcode
location: https://synbiohub.org/public/igem/BBa_E2050/annotation2214024/range2214024/1

https://synbiohub.org/public/igem/BBa_E2050/annotation2214024/range2214024/1
type: Range
persistent identity: range2214024
display id: range2214024
version: 1
direction: direction/forward
top level: mRFP1
start: 745
end: 769
orientation: inline

mOrange
type: SequenceAnnotation
persistent identity: annotation1431920
display id: annotation1431920
version: 1
top level: mRFP1
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_E2050/annotation1431920/range1431920/1

https://synbiohub.org/public/igem/BBa_E2050/annotation1431920/range1431920/1
type: Range
persistent identity: range1431920
display id: range1431920
version: 1
direction: direction/forward
top level: mRFP1
start: 1
end: 738
orientation: inline

BBa_K1510017
type: ComponentDefinition
persistent identity: BBa K1510017
display id: BBa_K1510017
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: red florescence protein RFC23
creator: Chien-Yung Huang
created: 2014-10-05T11:00:00Z
modified: 2016-01-26T02:09:57Z
discontinued: false
dominant: false
group u list: _1890_
m user id: 0
m user id: 4206
owner id: 21268
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: The part is basically a sequence encoding red florescent protein. With specially designed primers, it can be fused with its previous signal protein, yebF.
mutable notes: It is RFC23 compatible, with specially designed prefix.
mutable provenance: Obtaining this sequence, we designed primers to take the sequence from IGEM distributed part, BBa_E1010.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1510017_sequence/1

dTomato
type: ComponentDefinition
persistent identity: BBa K1688011
display id: BBa_K1688011
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: dTomato (inc RBS, J23110 promoter)
creator: Dianna Zeleskov
created: 2015-09-14T11:00:00Z
modified: 2015-09-18T11:31:20Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2106_
m user id: 27796
owner id: 27121
owning group id: 9
status: status/Planning
bookmark: false
mutable description: '''The dTomato protein is a fluorescent dimer protein that emits orange-red light when it is excited by green-yellow light. The excitation wavelengths and the emission wavelengths don't overlap as much as in other fluorescent proteins, which makes it preferable to use ??? especially in self‑made fluorometry tests.'''
mutable provenance: dTomato was derived from a monomeric mutant of the anthozoan coral RFP, DsRed.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_J23110
component: BBa_K1688004
sequence annotation: BBa_K1688004
sequence annotation: BBa_J23110
sequence: https://synbiohub.org/public/igem/BBa_K1688011_sequence/1

BBa_J23110
type: Component
persistent identity: component2457935
display id: component2457935
version: 1
top level: dTomato
access: public
definition: https://synbiohub.org/public/igem/BBa_J23110/1

BBa_K1688004
type: Component
persistent identity: component2457939
display id: component2457939
version: 1
top level: dTomato
access: public
definition: dTomato

BBa_K1688004
type: SequenceAnnotation
persistent identity: annotation2457939
display id: annotation2457939
version: 1
top level: dTomato
location: https://synbiohub.org/public/igem/BBa_K1688011/annotation2457939/range2457939/1
component: BBa_K1688004

https://synbiohub.org/public/igem/BBa_K1688011/annotation2457939/range2457939/1
type: Range
persistent identity: range2457939
display id: range2457939
version: 1
direction: direction/reverse
top level: dTomato
start: 44
end: 769
orientation: inline

BBa_J23110
type: SequenceAnnotation
persistent identity: annotation2457935
display id: annotation2457935
version: 1
top level: dTomato
location: https://synbiohub.org/public/igem/BBa_K1688011/annotation2457935/range2457935/1
component: BBa_J23110

https://synbiohub.org/public/igem/BBa_K1688011/annotation2457935/range2457935/1
type: Range
persistent identity: range2457935
display id: range2457935
version: 1
direction: direction/reverse
top level: dTomato
start: 1
end: 35
orientation: inline

BBa_K1122006
type: ComponentDefinition
persistent identity: BBa K1122006
display id: BBa_K1122006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: mCherry (BBa_J06504) compatible with Genabler assembly
creator: Lina Gasiūnaitė
created: 2013-08-12T11:00:00Z
modified: 2016-01-26T02:16:52Z
discontinued: false
dominant: false
group u list: _1433_
m user id: 4206
owner id: 18510
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: mCherry (BBa_J0654) part with inward facing EarI recognition sites at the ends compatible with Genabler assembly.
mutable notes: To be compatible with Genabler assembly, the part has inward-facing EarI sites at the ends. When assembled by BioBrick assembly, five N-terminal residues MetAlaSerSerSer- and one C-terminal Ala residue will be added to the original sequence of BBa_E0040.
mutable provenance: mCherry is based on the BioBrick BBa_J06504.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: mCherry
sequence annotation: stop
sequence annotation: start
sequence annotation: EarI site
sequence: https://synbiohub.org/public/igem/BBa_K1122006_sequence/1

mCherry
type: SequenceAnnotation
persistent identity: annotation2346320
display id: annotation2346320
version: 1
top level: BBa_K1122006
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1122006/annotation2346320/range2346320/1

https://synbiohub.org/public/igem/BBa_K1122006/annotation2346320/range2346320/1
type: Range
persistent identity: range2346320
display id: range2346320
version: 1
direction: direction/forward
top level: BBa_K1122006
start: 1
end: 727
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2334606
display id: annotation2334606
version: 1
top level: BBa_K1122006
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1122006/annotation2334606/range2334606/1

https://synbiohub.org/public/igem/BBa_K1122006/annotation2334606/range2334606/1
type: Range
persistent identity: range2334606
display id: range2334606
version: 1
direction: direction/forward
top level: BBa_K1122006
start: 724
end: 726
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2334604
display id: annotation2334604
version: 1
top level: BBa_K1122006
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1122006/annotation2334604/range2334604/1

https://synbiohub.org/public/igem/BBa_K1122006/annotation2334604/range2334604/1
type: Range
persistent identity: range2334604
display id: range2334604
version: 1
direction: direction/forward
top level: BBa_K1122006
start: 1
end: 3
orientation: inline

EarI site
type: SequenceAnnotation
persistent identity: annotation2334605
display id: annotation2334605
version: 1
top level: BBa_K1122006
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1122006/annotation2334605/range2334605/1

https://synbiohub.org/public/igem/BBa_K1122006/annotation2334605/range2334605/1
type: Range
persistent identity: range2334605
display id: range2334605
version: 1
direction: direction/forward
top level: BBa_K1122006
start: 6
end: 11
orientation: inline

EarI site
type: SequenceAnnotation
persistent identity: annotation2334607
display id: annotation2334607
version: 1
top level: BBa_K1122006
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1122006/annotation2334607/range2334607/1

https://synbiohub.org/public/igem/BBa_K1122006/annotation2334607/range2334607/1
type: Range
persistent identity: range2334607
display id: range2334607
version: 1
direction: direction/reverse
top level: BBa_K1122006
start: 725
end: 730
orientation: inline

mRFP
type: ComponentDefinition
persistent identity: BBa K1615089
display id: BBa_K1615089
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: mRFP RFC25
creator: Brooke Rothschild-Mancinelli
created: 2015-08-30T11:00:00Z
modified: 2016-01-25T11:25:49Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2032_
m user id: 4206
owner id: 25547
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Highly engineered monomeric mutant of RFP. Improved version of part BBa_E1010 to remove illegal AgeI sites and add RFC25 prefix and suffix.
mutable notes: Codon optimised for E. coli. RFC 25 compatible.
mutable provenance: mRFP highly engineered variant originally from Discosoma striata.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: mRFP
sequence: https://synbiohub.org/public/igem/BBa_K1615089_sequence/1

mRFP
type: SequenceAnnotation
persistent identity: annotation2440569
display id: annotation2440569
version: 1
top level: mRFP
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1615089/annotation2440569/range2440569/1

https://synbiohub.org/public/igem/BBa_K1615089/annotation2440569/range2440569/1
type: Range
persistent identity: range2440569
display id: range2440569
version: 1
direction: direction/forward
top level: mRFP
start: 1
end: 675
orientation: inline

BBa_K2040122
type: ComponentDefinition
persistent identity: BBa K2040122
display id: BBa_K2040122
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: KillerRed + NLS
creator: Chilam Poon
created: 2016-10-13T11:00:00Z
modified: 2016-10-19T01:35:29Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2507_
m user id: 30360
owner id: 30360
owning group id: 9
status: status/Planning
bookmark: false
mutable description: A SV40 nuclear localization signal was added to the phototoxic protein KillerRed.
mutable notes: NLS
mutable provenance: BBa_K1184000
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: KillerRed
sequence annotation: SV40 NLS
sequence: https://synbiohub.org/public/igem/BBa_K2040122_sequence/1

KillerRed
type: SequenceAnnotation
persistent identity: annotation2518117
display id: annotation2518117
version: 1
top level: BBa_K2040122
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K2040122/annotation2518117/range2518117/1

https://synbiohub.org/public/igem/BBa_K2040122/annotation2518117/range2518117/1
type: Range
persistent identity: range2518117
display id: range2518117
version: 1
direction: direction/forward
top level: BBa_K2040122
start: 1
end: 717
orientation: inline

SV40 NLS
type: SequenceAnnotation
persistent identity: annotation2502105
display id: annotation2502105
version: 1
top level: BBa_K2040122
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K2040122/annotation2502105/range2502105/1

https://synbiohub.org/public/igem/BBa_K2040122/annotation2502105/range2502105/1
type: Range
persistent identity: range2502105
display id: range2502105
version: 1
direction: direction/forward
top level: BBa_K2040122
start: 718
end: 741
orientation: inline