IP-Free BlazeYFP (Yellow Fluorescent Protein)
This part was created by DNA 2.0 as part of their IP-Free series of fluorescent and chromogenic proteins. It is available to use under the BioBrick Public Agreement. Excitation 515nm, Emission 534nm Molecular Weight: 29.2kDA Length: 263aa
This sequence is fully synthetic, (non-Aequorea) and is codon optimized for expression in E. coli.
This synthetic part was created through a gene engineering and synthesis project at DNA 2.0.
SuperYFP2 (BBa_K864100) with 6x His tag for protein purification.
Arabidopsis optimized Miraculin with a 2xYFP tag.
Miraculin was synthesized. YFP tag was added by cloning
A split protein is a protein whose sequence has been divided into two (or more) different parts. Often used to study protein-protein interactions, the protein can not perform its function until the parts are put back together. For instance, YFP, the yellow-fluorescent protein, will only express fluorescence when its two parts will be reunited. In normal condition, the production of a protein in response to a stimulus can easily reach several hours due to the many steps required for the protein synthesis. By using split-proteins, we are taking advantage of the absence of fluorescence when the two parts are apart. Indeed, the two parts of our split-YFP, when remaining separated, can be produced without being effective. Therefore, the overall process is far less time-consuming. However, to implement a light control on the fluorescence activation, a genetic construction gathering the VVD photoreceptor and our split-YFP has to be engineered. The new alternative approach for the visualization of protein interactiosn has been developed; the biomolecular fluorescence complementation (BiFC) techniques based on the complementation between fragments of fluorescent proteins; fragments of the yellow fluorescent protein (YFP) brought together by the association of two interaction partners fused to the fragments. They noticed that the spectral characteristics of BiFC of YFP were virtually identical to those of intact YFP.(Chang-Deng Hu, 2003)
Arabidopsis optimized Brazzein with a 2xYFP tag.
Brazzein was synthesized. YFP tag was added by cloning
YFP (BBa_K1491004) with 6X-His tag for easier protein purification. Sequence was codon optimized for E.coli via IDT codon optimizing tool.
Codon optimized for E.coli and 6X-His tag for easier protein purification.
mBanana fluorescent protein - Rights owned by Clontech. Sequence is commercially available from Clontech. Sequence obtained through PCR amplification, with BioBrick prefix and suffix added through in-house designed primers.
PstI site within the mBanana sequence - has to be assembled using BioBrick assembly in such an order that any successive digestions will not require PstI, otherwise the mCherry sequence will be cut as well as the site of interest. Alternatively, the assembly can be performed using non-standard BioBrick assembly. Part cannot be submitted to registry as the rights are owned by Clontech. The mCherry sequence is however commercially available from Clontech in the pmBanana vector.
Yellow Fluorescent Protein derived from A. victoria GFP. This fluorescent protein is cloned from the pZE12-YFP plasmid used by Elowitz (see reference). The original sequence was made by the The Yeast Resource Center (YRC) based at the University of Washington in Seattle, Washington.
This fluorescent protein was cloned from the pZE12-YFP plasmid used by Elowitz (see reference).
Elowitz, M. B., A. J. Levine, et al. (2002). "Stochastic gene expression in a single cell." Science 297(5584): 1183-6.
mVenus is a Chlamydomonas reinhardtii nuclear codon-optimized fluorescent protein. A variant of YFP, mVenus excites at 514nm and excites between 520 and 550nm. It includes two introns from RBCS2 in increase expression levels. Lauersen, Kyle., Kruse, Olaf., & Mussgnug Jan. "Targeted expression of nuclear transgenes in Chlamydomonas reinhardtii with a versatile, modular vector toolkit." Applied Microbiology Biotechnology. (2015) 99;3491-3503.
This part was adapted from pOopt_mVenus_Hyg and was originally from ChlamyCollection.org
Super Yellow Fluorescent Protein 2 (SYFP2) in RFC[25]
Improved part of BBa_K864100 which originally cannot be fused to other protein domains. This improved version is flanked by RFC[25] pre- and suffix for further protein fusions.
Super Yellow Fluorescent Protein 2 (SYFP2)
This part codes for the bright yellow fluorescent protein SYFP2. SYFP2 is a GFP based monomeric protein with a narrow fluorescence emission spectrum with a maximum at 515 nm. Bacteria expressing SYFP2 are reported to be 12 times brighter than those expressing EYFP(Q69K) and almost 2-fold brighter than bacteria expressing Venus (Ref. 10.1021/bi0516273). Codon optimized for expression in E coli by DNA 2.0. Mutations compared to wtGFP amino acid sequence (GenBank Accession number M62653) are F46L F64L S65G S72A M153T V163A S175G T203Y A206K.
The sequence has been codon optimized for expression in E coli by DNA 2.0.
Amino acid sequence taken from the article by Kremers et al 2006.