//cds/reporter/yfp
type: Collection
persistent identity: cds reporter yfp
display id: cds_reporter_yfp
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
owned by: james
owned by: myers
member: BBa_K180009
member: BBa_K1857000
member: BBa_K382040
member: BBa_K1418030
member: YFP-N
member: Venus
member: BBa_K1861030
member: BBa_K1323010
member: BBa_K1491004
member: BBa_K1732024
member: BBa_K382041
member: YFP
member: SYFP2
member: BBa_J97002
member: BBa_K1159301
member: YFP-C

BBa_K1323010
type: ComponentDefinition
persistent identity: BBa K1323010
display id: BBa_K1323010
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: YFP Coding Sequence
creator: Julia Manalil
created: 2014-10-03T11:00:00Z
modified: 2016-01-26T02:08:54Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1698_
m user id: 4206
owner id: 18368
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: TBA
mutable notes: TBA
mutable provenance: TBA
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: YFP
sequence: https://synbiohub.org/public/igem/BBa_K1323010_sequence/1

YFP
type: SequenceAnnotation
persistent identity: annotation2428779
display id: annotation2428779
version: 1
top level: BBa_K1323010
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1323010/annotation2428779/range2428779/1

https://synbiohub.org/public/igem/BBa_K1323010/annotation2428779/range2428779/1
type: Range
persistent identity: range2428779
display id: range2428779
version: 1
direction: direction/forward
top level: BBa_K1323010
start: 1
end: 720
orientation: inline

BBa_J97002
type: ComponentDefinition
persistent identity: BBa J97002
display id: BBa_J97002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: IP-Free BlazeYFP (Yellow Fluorescent Protein)
creator: Drew Endy
created: 2014-02-18T12:00:00Z
modified: 2015-05-08T01:08:32Z
discontinued: false
dominant: false
experience: experience/None
group u list: _41_1_
m user id: 0
owner id: 747
owning group id: 63
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This part was created by DNA 2.0 as part of their IP-Free series of fluorescent and chromogenic proteins. It is available to use under the BioBrick Public Agreement. Excitation 515nm, Emission 534nm Molecular Weight: 29.2kDA Length: 263aa
mutable notes: This sequence is fully synthetic, (non-Aequorea) and is codon optimized for expression in E. coli.
mutable provenance: This synthetic part was created through a gene engineering and synthesis project at DNA 2.0.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence: https://synbiohub.org/public/igem/BBa_J97002_sequence/1

BBa_K1491004
type: ComponentDefinition
persistent identity: BBa K1491004
display id: BBa_K1491004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: SuperYFP2-His
creator: Danielle Peters
created: 2014-07-14T11:00:00Z
modified: 2016-01-21T02:28:40Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1871_
m user id: 4206
owner id: 22521
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: SuperYFP2 (BBa_K864100) with 6x His tag for protein purification.
mutable notes: YFP+His
mutable provenance: SuperYFP2 is BBa_K864100
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: 6x His
sequence annotation: BBa_K864100
sequence: https://synbiohub.org/public/igem/BBa_K1491004_sequence/1

6x His
type: SequenceAnnotation
persistent identity: annotation2380095
display id: annotation2380095
version: 1
top level: BBa_K1491004
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1491004/annotation2380095/range2380095/1

https://synbiohub.org/public/igem/BBa_K1491004/annotation2380095/range2380095/1
type: Range
persistent identity: range2380095
display id: range2380095
version: 1
direction: direction/forward
top level: BBa_K1491004
start: 718
end: 735
orientation: inline

BBa_K864100
type: SequenceAnnotation
persistent identity: annotation2380094
display id: annotation2380094
version: 1
top level: BBa_K1491004
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1491004/annotation2380094/range2380094/1

https://synbiohub.org/public/igem/BBa_K1491004/annotation2380094/range2380094/1
type: Range
persistent identity: range2380094
display id: range2380094
version: 1
direction: direction/forward
top level: BBa_K1491004
start: 1
end: 717
orientation: inline

BBa_K382040
type: ComponentDefinition
persistent identity: BBa K382040
display id: BBa_K382040
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Arabidopsis optimized Miraculin+2xYFP
creator: Mara C. Inniss
created: 2010-10-25T11:00:00Z
modified: 2015-06-11T01:06:36Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _488_
m user id: 4206
owner id: 7454
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Arabidopsis optimized Miraculin with a 2xYFP tag.
mutable notes: Conforms to Silver standard 21
mutable provenance: Miraculin was synthesized. YFP tag was added by cloning
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Arabidopsis optimized Miraculin
sequence annotation: 2xYFP
sequence annotation: biobrick scar (standard 23)
sequence annotation: start codon
sequence: https://synbiohub.org/public/igem/BBa_K382040_sequence/1

Arabidopsis optimized Miraculin
type: SequenceAnnotation
persistent identity: annotation2114287
display id: annotation2114287
version: 1
top level: BBa_K382040
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K382040/annotation2114287/range2114287/1

https://synbiohub.org/public/igem/BBa_K382040/annotation2114287/range2114287/1
type: Range
persistent identity: range2114287
display id: range2114287
version: 1
direction: direction/forward
top level: BBa_K382040
start: 1
end: 660
orientation: inline

2xYFP
type: SequenceAnnotation
persistent identity: annotation2114289
display id: annotation2114289
version: 1
top level: BBa_K382040
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K382040/annotation2114289/range2114289/1

https://synbiohub.org/public/igem/BBa_K382040/annotation2114289/range2114289/1
type: Range
persistent identity: range2114289
display id: range2114289
version: 1
direction: direction/forward
top level: BBa_K382040
start: 667
end: 2160
orientation: inline

biobrick scar (standard 23)
type: SequenceAnnotation
persistent identity: annotation2114288
display id: annotation2114288
version: 1
top level: BBa_K382040
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K382040/annotation2114288/range2114288/1

https://synbiohub.org/public/igem/BBa_K382040/annotation2114288/range2114288/1
type: Range
persistent identity: range2114288
display id: range2114288
version: 1
direction: direction/forward
top level: BBa_K382040
start: 661
end: 666
orientation: inline

start codon
type: SequenceAnnotation
persistent identity: annotation2114286
display id: annotation2114286
version: 1
top level: BBa_K382040
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K382040/annotation2114286/range2114286/1

https://synbiohub.org/public/igem/BBa_K382040/annotation2114286/range2114286/1
type: Range
persistent identity: range2114286
display id: range2114286
version: 1
direction: direction/forward
top level: BBa_K382040
start: 1
end: 3
orientation: inline

YFP-N
type: ComponentDefinition
persistent identity: BBa K1616017
display id: BBa_K1616017
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: YN155 - N terminal YFP split
creator: Johanna Chesnel
created: 2015-09-17T11:00:00Z
modified: 2016-01-21T02:25:38Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2033_
m user id: 4206
owner id: 22805
owning group id: 9
status: status/Planning
bookmark: false
mutable description: A split protein is a protein whose sequence has been divided into two (or more) different parts. Often used to study protein-protein interactions, the protein can not perform its function until the parts are put back together. For instance, YFP, the yellow-fluorescent protein, will only express fluorescence when its two parts will be reunited. In normal condition, the production of a protein in response to a stimulus can easily reach several hours due to the many steps required for the protein synthesis. By using split-proteins, we are taking advantage of the absence of fluorescence when the two parts are apart. Indeed, the two parts of our split-YFP, when remaining separated, can be produced without being effective. Therefore, the overall process is far less time-consuming. However, to implement a light control on the fluorescence activation, a genetic construction gathering the VVD photoreceptor and our split-YFP has to be engineered. The new alternative approach for the visualization of protein interactiosn has been developed; the biomolecular fluorescence complementation (BiFC) techniques based on the complementation between fragments of fluorescent proteins; fragments of the yellow fluorescent protein (YFP) brought together by the association of two interaction partners fused to the fragments. They noticed that the spectral characteristics of BiFC of YFP were virtually identical to those of intact YFP.(Chang-Deng Hu, 2003)
mutable notes: Illegal sites have been checked.
mutable provenance: This part works with BBa_K16160016
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: YN155
sequence: https://synbiohub.org/public/igem/BBa_K1616017_sequence/1

YN155
type: SequenceAnnotation
persistent identity: annotation2470148
display id: annotation2470148
version: 1
top level: YFP-N
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1616017/annotation2470148/range2470148/1

https://synbiohub.org/public/igem/BBa_K1616017/annotation2470148/range2470148/1
type: Range
persistent identity: range2470148
display id: range2470148
version: 1
direction: direction/forward
top level: YFP-N
start: 1
end: 468
orientation: inline

BBa_K382041
type: ComponentDefinition
persistent identity: BBa K382041
display id: BBa_K382041
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Arabidopsis optimized Brazzein+2xYFP
creator: Mara C. Inniss
created: 2010-10-25T11:00:00Z
modified: 2015-06-11T01:07:07Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _488_
m user id: 4206
owner id: 7454
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Arabidopsis optimized Brazzein with a 2xYFP tag.
mutable notes: Conforms to Silver standard 21
mutable provenance: Brazzein was synthesized. YFP tag was added by cloning
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: 2xYFP
sequence annotation: start codon
sequence annotation: biobrick scar (standard 23)
sequence annotation: Arabidopsis optimized Brazzein
sequence: https://synbiohub.org/public/igem/BBa_K382041_sequence/1

2xYFP
type: SequenceAnnotation
persistent identity: annotation2114278
display id: annotation2114278
version: 1
top level: BBa_K382041
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K382041/annotation2114278/range2114278/1

https://synbiohub.org/public/igem/BBa_K382041/annotation2114278/range2114278/1
type: Range
persistent identity: range2114278
display id: range2114278
version: 1
direction: direction/forward
top level: BBa_K382041
start: 172
end: 1665
orientation: inline

start codon
type: SequenceAnnotation
persistent identity: annotation2114275
display id: annotation2114275
version: 1
top level: BBa_K382041
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K382041/annotation2114275/range2114275/1

https://synbiohub.org/public/igem/BBa_K382041/annotation2114275/range2114275/1
type: Range
persistent identity: range2114275
display id: range2114275
version: 1
direction: direction/forward
top level: BBa_K382041
start: 1
end: 3
orientation: inline

biobrick scar (standard 23)
type: SequenceAnnotation
persistent identity: annotation2114277
display id: annotation2114277
version: 1
top level: BBa_K382041
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K382041/annotation2114277/range2114277/1

https://synbiohub.org/public/igem/BBa_K382041/annotation2114277/range2114277/1
type: Range
persistent identity: range2114277
display id: range2114277
version: 1
direction: direction/forward
top level: BBa_K382041
start: 166
end: 171
orientation: inline

Arabidopsis optimized Brazzein
type: SequenceAnnotation
persistent identity: annotation2114276
display id: annotation2114276
version: 1
top level: BBa_K382041
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K382041/annotation2114276/range2114276/1

https://synbiohub.org/public/igem/BBa_K382041/annotation2114276/range2114276/1
type: Range
persistent identity: range2114276
display id: range2114276
version: 1
direction: direction/forward
top level: BBa_K382041
start: 1
end: 165
orientation: inline

BBa_K1732024
type: ComponentDefinition
persistent identity: BBa K1732024
display id: BBa_K1732024
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: YFPCO-His
creator: Donna Lee
created: 2015-09-17T11:00:00Z
modified: 2016-01-21T02:27:14Z
discontinued: false
dominant: false
group u list: _2154_
m user id: 4206
owner id: 27195
owning group id: 9
status: status/Planning
bookmark: false
mutable description: YFP (BBa_K1491004) with 6X-His tag for easier protein purification. Sequence was codon optimized for E.coli via IDT codon optimizing tool.
mutable notes: Codon optimized for E.coli and 6X-His tag for easier protein purification.
mutable provenance: BBa_K1491004
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: YFPCO
sequence annotation: 6X-His
sequence: https://synbiohub.org/public/igem/BBa_K1732024_sequence/1

YFPCO
type: SequenceAnnotation
persistent identity: annotation2469348
display id: annotation2469348
version: 1
top level: BBa_K1732024
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1732024/annotation2469348/range2469348/1

https://synbiohub.org/public/igem/BBa_K1732024/annotation2469348/range2469348/1
type: Range
persistent identity: range2469348
display id: range2469348
version: 1
direction: direction/forward
top level: BBa_K1732024
start: 1
end: 716
orientation: inline

6X-His
type: SequenceAnnotation
persistent identity: annotation2469350
display id: annotation2469350
version: 1
top level: BBa_K1732024
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1732024/annotation2469350/range2469350/1

https://synbiohub.org/public/igem/BBa_K1732024/annotation2469350/range2469350/1
type: Range
persistent identity: range2469350
display id: range2469350
version: 1
direction: direction/forward
top level: BBa_K1732024
start: 717
end: 735
orientation: inline

BBa_K180009
type: ComponentDefinition
persistent identity: BBa K180009
display id: BBa_K180009
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: mBanana (rights owned by Clontech)
creator: Richard Wilson, Stephanie Chambers
created: 2009-10-15T11:00:00Z
modified: 2016-01-25T01:37:03Z
discontinued: false
dominant: false
experience: experience/None
group u list: _278_
m user id: 4206
owner id: 4082
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: mBanana fluorescent protein - Rights owned by Clontech. Sequence is commercially available from Clontech. Sequence obtained through PCR amplification, with BioBrick prefix and suffix added through in-house designed primers.
mutable notes: PstI site within the mBanana sequence - has to be assembled using BioBrick assembly in such an order that any successive digestions will not require PstI, otherwise the mCherry sequence will be cut as well as the site of interest. Alternatively, the assembly can be performed using non-standard BioBrick assembly. Part cannot be submitted to registry as the rights are owned by Clontech. The mCherry sequence is however commercially available from Clontech in the pmBanana vector.
mutable provenance: pmBanana vector, Clontech
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: mBanana
sequence: https://synbiohub.org/public/igem/BBa_K180009_sequence/1

mBanana
type: SequenceAnnotation
persistent identity: annotation2046843
display id: annotation2046843
version: 1
top level: BBa_K180009
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K180009/annotation2046843/range2046843/1

https://synbiohub.org/public/igem/BBa_K180009/annotation2046843/range2046843/1
type: Range
persistent identity: range2046843
display id: range2046843
version: 1
direction: direction/forward
top level: BBa_K180009
start: 1
end: 708
orientation: inline

Venus
type: ComponentDefinition
persistent identity: BBa K1907000
display id: BBa_K1907000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Venus Yellow Fluorescent Protein
creator: Pihla Savola
created: 2016-10-11T11:00:00Z
modified: 2016-10-19T12:26:01Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2373_
m user id: 29559
owner id: 29693
owning group id: 9
status: status/Planning
bookmark: false
mutable description: -
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1907000_sequence/1

BBa_K1418030
type: ComponentDefinition
persistent identity: BBa K1418030
display id: BBa_K1418030
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: SYFP2
creator: Cody Maxfield
created: 2014-10-07T11:00:00Z
modified: 2016-01-26T02:11:39Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1796_
m user id: 4206
owner id: 13965
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: SYFP2
mutable notes: RFC 23
mutable provenance: SYFP2
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: atg
sequence annotation: SYFP2
sequence annotation: taataa
sequence: https://synbiohub.org/public/igem/BBa_K1418030_sequence/1

atg
type: SequenceAnnotation
persistent identity: annotation2421320
display id: annotation2421320
version: 1
top level: BBa_K1418030
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1418030/annotation2421320/range2421320/1

https://synbiohub.org/public/igem/BBa_K1418030/annotation2421320/range2421320/1
type: Range
persistent identity: range2421320
display id: range2421320
version: 1
direction: direction/forward
top level: BBa_K1418030
start: 1
end: 3
orientation: inline

SYFP2
type: SequenceAnnotation
persistent identity: annotation2421321
display id: annotation2421321
version: 1
top level: BBa_K1418030
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1418030/annotation2421321/range2421321/1

https://synbiohub.org/public/igem/BBa_K1418030/annotation2421321/range2421321/1
type: Range
persistent identity: range2421321
display id: range2421321
version: 1
direction: direction/forward
top level: BBa_K1418030
start: 4
end: 717
orientation: inline

taataa
type: SequenceAnnotation
persistent identity: annotation2421322
display id: annotation2421322
version: 1
top level: BBa_K1418030
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1418030/annotation2421322/range2421322/1

https://synbiohub.org/public/igem/BBa_K1418030/annotation2421322/range2421322/1
type: Range
persistent identity: range2421322
display id: range2421322
version: 1
direction: direction/forward
top level: BBa_K1418030
start: 718
end: 723
orientation: inline

BBa_K1861030
type: ComponentDefinition
persistent identity: BBa K1861030
display id: BBa_K1861030
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: eYFP gene for expression in yeast
creator: Vladyslav Vyshnevskyi
created: 2015-09-17T11:00:00Z
modified: 2015-09-23T04:49:52Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2291_
m user id: 25348
owner id: 25348
owning group id: 9
status: status/Planning
bookmark: true
mutable description: Coming soon.
mutable notes: Coming soon.
mutable provenance: Coming soon.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_J63002
component: BBa_K1861304
component: BBa_K792001
component: BBa_K1861302
component: BBa_K1861303
component: BBa_K1861301
sequence annotation: BBa_K1861302
sequence annotation: BBa_J63002
sequence annotation: BBa_K792001
sequence annotation: BBa_K1861304
sequence annotation: BBa_K1861301
sequence annotation: BBa_K1861303
sequence: https://synbiohub.org/public/igem/BBa_K1861030_sequence/1

BBa_J63002
type: Component
persistent identity: component2474915
display id: component2474915
version: 1
top level: BBa_K1861030
access: public
definition: https://synbiohub.org/public/igem/BBa_J63002/1

BBa_K1861304
type: Component
persistent identity: component2474916
display id: component2474916
version: 1
top level: BBa_K1861030
access: public
definition: https://synbiohub.org/public/igem/BBa_K1861304/1

BBa_K792001
type: Component
persistent identity: component2474912
display id: component2474912
version: 1
top level: BBa_K1861030
access: public
definition: https://synbiohub.org/public/igem/BBa_K792001/1

BBa_K1861302
type: Component
persistent identity: component2474913
display id: component2474913
version: 1
top level: BBa_K1861030
access: public
definition: https://synbiohub.org/public/igem/BBa_K1861302/1

BBa_K1861303
type: Component
persistent identity: component2474908
display id: component2474908
version: 1
top level: BBa_K1861030
access: public
definition: https://synbiohub.org/public/igem/BBa_K1861303/1

BBa_K1861301
type: Component
persistent identity: component2474907
display id: component2474907
version: 1
top level: BBa_K1861030
access: public
definition: https://synbiohub.org/public/igem/BBa_K1861301/1

BBa_K1861302
type: SequenceAnnotation
persistent identity: annotation2474913
display id: annotation2474913
version: 1
top level: BBa_K1861030
location: https://synbiohub.org/public/igem/BBa_K1861030/annotation2474913/range2474913/1
component: BBa_K1861302

https://synbiohub.org/public/igem/BBa_K1861030/annotation2474913/range2474913/1
type: Range
persistent identity: range2474913
display id: range2474913
version: 1
direction: direction/reverse
top level: BBa_K1861030
start: 297
end: 1061
orientation: inline

BBa_J63002
type: SequenceAnnotation
persistent identity: annotation2474915
display id: annotation2474915
version: 1
top level: BBa_K1861030
location: https://synbiohub.org/public/igem/BBa_K1861030/annotation2474915/range2474915/1
component: BBa_J63002

https://synbiohub.org/public/igem/BBa_K1861030/annotation2474915/range2474915/1
type: Range
persistent identity: range2474915
display id: range2474915
version: 1
direction: direction/reverse
top level: BBa_K1861030
start: 1062
end: 1286
orientation: inline

BBa_K792001
type: SequenceAnnotation
persistent identity: annotation2474912
display id: annotation2474912
version: 1
top level: BBa_K1861030
location: https://synbiohub.org/public/igem/BBa_K1861030/annotation2474912/range2474912/1
component: BBa_K792001

https://synbiohub.org/public/igem/BBa_K1861030/annotation2474912/range2474912/1
type: Range
persistent identity: range2474912
display id: range2474912
version: 1
direction: direction/reverse
top level: BBa_K1861030
start: 276
end: 296
orientation: inline

BBa_K1861304
type: SequenceAnnotation
persistent identity: annotation2474916
display id: annotation2474916
version: 1
top level: BBa_K1861030
location: https://synbiohub.org/public/igem/BBa_K1861030/annotation2474916/range2474916/1
component: BBa_K1861304

https://synbiohub.org/public/igem/BBa_K1861030/annotation2474916/range2474916/1
type: Range
persistent identity: range2474916
display id: range2474916
version: 1
direction: direction/reverse
top level: BBa_K1861030
start: 1287
end: 1349
orientation: inline

BBa_K1861301
type: SequenceAnnotation
persistent identity: annotation2474907
display id: annotation2474907
version: 1
top level: BBa_K1861030
location: https://synbiohub.org/public/igem/BBa_K1861030/annotation2474907/range2474907/1
component: BBa_K1861301

https://synbiohub.org/public/igem/BBa_K1861030/annotation2474907/range2474907/1
type: Range
persistent identity: range2474907
display id: range2474907
version: 1
direction: direction/reverse
top level: BBa_K1861030
start: 1
end: 71
orientation: inline

BBa_K1861303
type: SequenceAnnotation
persistent identity: annotation2474908
display id: annotation2474908
version: 1
top level: BBa_K1861030
location: https://synbiohub.org/public/igem/BBa_K1861030/annotation2474908/range2474908/1
component: BBa_K1861303

https://synbiohub.org/public/igem/BBa_K1861030/annotation2474908/range2474908/1
type: Range
persistent identity: range2474908
display id: range2474908
version: 1
direction: direction/reverse
top level: BBa_K1861030
start: 72
end: 275
orientation: inline

YFP
type: ComponentDefinition
persistent identity: BBa K592101
display id: BBa_K592101
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Yellow Fluorescent Protein (YFP)
creator: Erik Lundin
created: 2011-09-20T11:00:00Z
modified: 2016-01-25T04:47:08Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _763_
m user id: 4206
owner id: 9827
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: Yellow Fluorescent Protein derived from A. victoria GFP. This fluorescent protein is cloned from the pZE12-YFP plasmid used by Elowitz (see reference). The original sequence was made by the The Yeast Resource Center (YRC) based at the University of Washington in Seattle, Washington.
mutable notes: This fluorescent protein was cloned from the pZE12-YFP plasmid used by Elowitz (see reference).
mutable provenance: Elowitz, M. B., A. J. Levine, et al. (2002). "Stochastic gene expression in a single cell." Science 297(5584): 1183-6.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: YFP
sequence: https://synbiohub.org/public/igem/BBa_K592101_sequence/1

YFP
type: SequenceAnnotation
persistent identity: annotation2137506
display id: annotation2137506
version: 1
top level: YFP
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K592101/annotation2137506/range2137506/1

https://synbiohub.org/public/igem/BBa_K592101/annotation2137506/range2137506/1
type: Range
persistent identity: range2137506
display id: range2137506
version: 1
direction: direction/forward
top level: YFP
start: 1
end: 714
orientation: inline

BBa_K1857000
type: ComponentDefinition
persistent identity: BBa K1857000
display id: BBa_K1857000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: mVenus
creator: Joshua Timmons
created: 2015-09-15T11:00:00Z
modified: 2016-01-25T01:12:03Z
discontinued: false
dominant: false
group u list: _2285_
m user id: 4206
owner id: 26495
owning group id: 9
status: status/Planning
bookmark: false
mutable description: mVenus is a Chlamydomonas reinhardtii nuclear codon-optimized fluorescent protein. A variant of YFP, mVenus excites at 514nm and excites between 520 and 550nm. It includes two introns from RBCS2 in increase expression levels. Lauersen, Kyle., Kruse, Olaf., & Mussgnug Jan. "Targeted expression of nuclear transgenes in Chlamydomonas reinhardtii with a versatile, modular vector toolkit." Applied Microbiology Biotechnology. (2015) 99;3491-3503.
mutable notes: N/A
mutable provenance: This part was adapted from pOopt_mVenus_Hyg and was originally from ChlamyCollection.org
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: mVenus
sequence: https://synbiohub.org/public/igem/BBa_K1857000_sequence/1

mVenus
type: SequenceAnnotation
persistent identity: annotation2473680
display id: annotation2473680
version: 1
top level: BBa_K1857000
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1857000/annotation2473680/range2473680/1

https://synbiohub.org/public/igem/BBa_K1857000/annotation2473680/range2473680/1
type: Range
persistent identity: range2473680
display id: range2473680
version: 1
direction: direction/forward
top level: BBa_K1857000
start: 144
end: 1236
orientation: inline

YFP-C
type: ComponentDefinition
persistent identity: BBa K1616016
display id: BBa_K1616016
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: YC155 - C terminal YFP split
creator: Johanna Chesnel
created: 2015-09-17T11:00:00Z
modified: 2016-01-21T02:25:51Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2033_
m user id: 4206
owner id: 22805
owning group id: 9
status: status/Planning
bookmark: false
mutable description: A split protein is a protein whose sequence has been divided into two (or more) different parts. Often used to study protein-protein interactions, the protein can not perform its function until the parts are put back together. For instance, YFP, the yellow-fluorescent protein, will only express fluorescence when its two parts will be reunited. In normal condition, the production of a protein in response to a stimulus can easily reach several hours due to the many steps required for the protein synthesis. By using split-proteins, we are taking advantage of the absence of fluorescence when the two parts are apart. Indeed, the two parts of our split-YFP, when remaining separated, can be produced without being effective. Therefore, the overall process is far less time-consuming. However, to implement a light control on the fluorescence activation, a genetic construction gathering the VVD photoreceptor and our split-YFP has to be engineered. The new alternative approach for the visualization of protein interactiosn has been developed; the biomolecular fluorescence complementation (BiFC) techniques based on the complementation between fragments of fluorescent proteins; fragments of the yellow fluorescent protein (YFP) brought together by the association of two interaction partners fused to the fragments. They noticed that the spectral characteristics of BiFC of YFP were virtually identical to those of intact YFP.(Chang-Deng Hu, 2003)
mutable notes: Illegal sites have been checked.
mutable provenance: This part works with BBa_K16160017.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: YC155
sequence: https://synbiohub.org/public/igem/BBa_K1616016_sequence/1

YC155
type: SequenceAnnotation
persistent identity: annotation2470135
display id: annotation2470135
version: 1
top level: YFP-C
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1616016/annotation2470135/range2470135/1

https://synbiohub.org/public/igem/BBa_K1616016/annotation2470135/range2470135/1
type: Range
persistent identity: range2470135
display id: range2470135
version: 1
direction: direction/forward
top level: YFP-C
start: 1
end: 255
orientation: inline

BBa_K1159301
type: ComponentDefinition
persistent identity: BBa K1159301
display id: BBa_K1159301
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Super Yellow Fluorescent Protein 2 (SYFP2) in RFC[25]
creator: Dong-Jiunn Jeffery TRUONG
created: 2013-09-13T11:00:00Z
modified: 2016-01-26T02:17:14Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1471_
m user id: 4206
owner id: 11507
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: Improved part of BBa_K864100 which originally cannot be fused to other protein domains. This improved version is flanked by RFC[25] pre- and suffix for further protein fusions.
mutable notes: x
mutable provenance: ''Aequorea victoria''
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: SYFP2
sequence: https://synbiohub.org/public/igem/BBa_K1159301_sequence/1

SYFP2
type: SequenceAnnotation
persistent identity: annotation2343586
display id: annotation2343586
version: 1
top level: BBa_K1159301
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1159301/annotation2343586/range2343586/1

https://synbiohub.org/public/igem/BBa_K1159301/annotation2343586/range2343586/1
type: Range
persistent identity: range2343586
display id: range2343586
version: 1
direction: direction/forward
top level: BBa_K1159301
start: 1
end: 714
orientation: inline

SYFP2
type: ComponentDefinition
persistent identity: BBa K864100
display id: BBa_K864100
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Super Yellow Fluorescent Protein 2 (SYFP2)
creator: Erik Gullberg
created: 2012-09-23T11:00:00Z
modified: 2016-01-21T02:29:24Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1124_
m user id: 4206
owner id: 10137
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This part codes for the bright yellow fluorescent protein SYFP2. SYFP2 is a GFP based monomeric protein with a narrow fluorescence emission spectrum with a maximum at 515 nm. Bacteria expressing SYFP2 are reported to be 12 times brighter than those expressing EYFP(Q69K) and almost 2-fold brighter than bacteria expressing Venus (Ref. 10.1021/bi0516273). Codon optimized for expression in E coli by DNA 2.0. Mutations compared to wtGFP amino acid sequence (GenBank Accession number M62653) are F46L F64L S65G S72A M153T V163A S175G T203Y A206K.
mutable notes: The sequence has been codon optimized for expression in E coli by DNA 2.0.
mutable provenance: Amino acid sequence taken from the article by Kremers et al 2006.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: SYFP2
sequence: https://synbiohub.org/public/igem/BBa_K864100_sequence/1

SYFP2
type: SequenceAnnotation
persistent identity: annotation2196221
display id: annotation2196221
version: 1
top level: SYFP2
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K864100/annotation2196221/range2196221/1

https://synbiohub.org/public/igem/BBa_K864100/annotation2196221/range2196221/1
type: Range
persistent identity: range2196221
display id: range2196221
version: 1
direction: direction/forward
top level: SYFP2
start: 1
end: 717
orientation: inline