//cds/selectionmarker/antibioticresistance
type: Collection
persistent identity: cds selectionmarker antibioticresistance
display id: cds_selectionmarker_antibioticresistance
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
owned by: james
owned by: myers
member: hygR
member: BBa_K1930003
member: BBa_K1015002
member: BBa_K1486027
member: CmR
member: BBa_K1207001
member: ptrA
member: BBa_K1015009
member: KanR Backw
member: BBa_K1323013
member: BBa_J23012
member: ampR
member: tetA(C)f
member: BBa_K319041
member: BBa_K1323012
member: BBa_K1021003
member: BBa_K1486029
member: BBa_K1486036
member: Aad9
member: kanR
member: BBa_K678022
member: BBa_K389011
member: BBa_K1486035
member: CiproR
member: BBa_K1323011
member: BBa_K156011
member: BBa_K678047
member: BBa_K1071001
member: BBa_T9010
member: BBa_K914018
member: ble
member: tetA(C)b
member: BBa_K1930007
member: BBa_K1015010
member: BBa_K1486033
member: BBa_K1486024
member: BBa_K1930002
member: Ori+Kan
member: BBa_K823020
member: NeoR
member: BBa_J133003
member: bleR
member: BBa_T9000
member: BBa_K914009
member: KanR
member: BBa_K394000
member: BBa_J133653
member: BBa_K1486030
member: CmR Backwa
member: BBa_K1486026

BBa_K394000
type: ComponentDefinition
persistent identity: BBa K394000
display id: BBa_K394000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Resistance Gene for G418 in Yeast
creator: Brian Landry
created: 2010-10-17T11:00:00Z
modified: 2015-05-08T01:12:21Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _504_
m user id: 0
owner id: 6327
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This part confers resistance to Geneticin (G418) in yeast. Selection of yeast cells is typically preformed on plates with 200??g/ml of G418.
mutable notes: None
mutable provenance: The part was synthesized from the PMKV005 plasmid used in McCusker et. al. The coding region contains the KanR open reading from transposon Tn903 from E. coli. 1. McCusker JH, Vorachek-Warren MK. DsdA (D-serine deaminase): a new heterologous MX cassette for gene disruption and selection in Saccharomyces cerevisiae. Yeast (Chichester, England). 2004;21(2):163-71. Available at: http://www.ncbi.nlm.nih.gov/pubmed/14755641.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Start Codon
sequence annotation: Stop
sequence annotation: KanR gene
sequence annotation: HindIII cut site
sequence: https://synbiohub.org/public/igem/BBa_K394000_sequence/1

Start Codon
type: SequenceAnnotation
persistent identity: annotation2104020
display id: annotation2104020
version: 1
top level: BBa_K394000
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K394000/annotation2104020/range2104020/1

https://synbiohub.org/public/igem/BBa_K394000/annotation2104020/range2104020/1
type: Range
persistent identity: range2104020
display id: range2104020
version: 1
direction: direction/forward
top level: BBa_K394000
start: 1
end: 3
orientation: inline

Stop
type: SequenceAnnotation
persistent identity: annotation2104023
display id: annotation2104023
version: 1
top level: BBa_K394000
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K394000/annotation2104023/range2104023/1

https://synbiohub.org/public/igem/BBa_K394000/annotation2104023/range2104023/1
type: Range
persistent identity: range2104023
display id: range2104023
version: 1
direction: direction/forward
top level: BBa_K394000
start: 808
end: 813
orientation: inline

KanR gene
type: SequenceAnnotation
persistent identity: annotation2104021
display id: annotation2104021
version: 1
top level: BBa_K394000
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K394000/annotation2104021/range2104021/1

https://synbiohub.org/public/igem/BBa_K394000/annotation2104021/range2104021/1
type: Range
persistent identity: range2104021
display id: range2104021
version: 1
direction: direction/forward
top level: BBa_K394000
start: 1
end: 810
orientation: inline

HindIII cut site
type: SequenceAnnotation
persistent identity: annotation2104022
display id: annotation2104022
version: 1
top level: BBa_K394000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K394000/annotation2104022/range2104022/1

https://synbiohub.org/public/igem/BBa_K394000/annotation2104022/range2104022/1
type: Range
persistent identity: range2104022
display id: range2104022
version: 1
direction: direction/forward
top level: BBa_K394000
start: 545
end: 549
orientation: inline

bleR
type: ComponentDefinition
persistent identity: BBa K678044
display id: BBa_K678044
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: bleR cassette, selection marker for Aspergillus nidulans
creator: DTU-Denmark-2
created: 2011-09-04T11:00:00Z
modified: 2015-05-08T01:13:04Z
discontinued: false
dominant: false
experience: experience/None
group u list: _882_
m user id: 0
owner id: 8341
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: bleR cassette, antibiotic selection marker for Aspergillus nidulans
mutable notes: Compatible with the Plug'n'Play assembly standard
mutable provenance: pAN8-1
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Linker 5
sequence annotation: bleR cassette
sequence: https://synbiohub.org/public/igem/BBa_K678044_sequence/1

Linker 5
type: SequenceAnnotation
persistent identity: annotation2127419
display id: annotation2127419
version: 1
top level: bleR
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K678044/annotation2127419/range2127419/1

https://synbiohub.org/public/igem/BBa_K678044/annotation2127419/range2127419/1
type: Range
persistent identity: range2127419
display id: range2127419
version: 1
direction: direction/forward
top level: bleR
start: 1
end: 8
orientation: inline

bleR cassette
type: SequenceAnnotation
persistent identity: annotation2127420
display id: annotation2127420
version: 1
top level: bleR
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K678044/annotation2127420/range2127420/1

https://synbiohub.org/public/igem/BBa_K678044/annotation2127420/range2127420/1
type: Range
persistent identity: range2127420
display id: range2127420
version: 1
direction: direction/forward
top level: bleR
start: 9
end: 1663
orientation: inline

BBa_J133653
type: ComponentDefinition
persistent identity: BBa J133653
display id: BBa_J133653
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: aadA Cassette + Flanked by TLTerminators (missing RBS)
creator: Norman Wang
created: 2011-07-21T11:00:00Z
modified: 2015-08-31T04:08:30Z
discontinued: false
dominant: false
experience: experience/None
group u list: _634_
m user id: 0
owner id: 2680
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: KanR gene BBa_P1003 with BsmBI removed, it has duplicated promoter region for kanR since it was amplified from pSB3K5m which is a pSB3K5 derivative. Flanked with bidirectional translational Terminators BBa_J133422 (prefix) and BBa_B0042 (postfix)
mutable notes: n/a
mutable provenance: http://partsregistry.org/wiki/index.php/Part:BBa_J133003
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000110
role: partType/Temporary
sequence annotation: BBa K125520 Aminoglycoside 3"-Adenylyltransferase (aadA1) Gene CDS
sequence annotation: BBa_J133422
sequence annotation: BBa_B0042
sequence annotation: E81V mutation GAG to GTG
sequence annotation: BBa I14032 P(Lac) IQ
sequence: https://synbiohub.org/public/igem/BBa_J133653_sequence/1

BBa K125520 Aminoglycoside 3"-Adenylyltransferase (aadA1) Gene CDS
type: SequenceAnnotation
persistent identity: annotation2125652
display id: annotation2125652
version: 1
top level: BBa_J133653
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J133653/annotation2125652/range2125652/1

https://synbiohub.org/public/igem/BBa_J133653/annotation2125652/range2125652/1
type: Range
persistent identity: range2125652
display id: range2125652
version: 1
direction: direction/forward
top level: BBa_J133653
start: 56
end: 847
orientation: inline

BBa_J133422
type: SequenceAnnotation
persistent identity: annotation2125650
display id: annotation2125650
version: 1
top level: BBa_J133653
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_J133653/annotation2125650/range2125650/1

https://synbiohub.org/public/igem/BBa_J133653/annotation2125650/range2125650/1
type: Range
persistent identity: range2125650
display id: range2125650
version: 1
direction: direction/forward
top level: BBa_J133653
start: 1
end: 12
orientation: inline

BBa_B0042
type: SequenceAnnotation
persistent identity: annotation2125653
display id: annotation2125653
version: 1
top level: BBa_J133653
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_J133653/annotation2125653/range2125653/1

https://synbiohub.org/public/igem/BBa_J133653/annotation2125653/range2125653/1
type: Range
persistent identity: range2125653
display id: range2125653
version: 1
direction: direction/forward
top level: BBa_J133653
start: 848
end: 859
orientation: inline

E81V mutation GAG to GTG
type: SequenceAnnotation
persistent identity: annotation2125654
display id: annotation2125654
version: 1
top level: BBa_J133653
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_J133653/annotation2125654/range2125654/1

https://synbiohub.org/public/igem/BBa_J133653/annotation2125654/range2125654/1
type: Range
persistent identity: range2125654
display id: range2125654
version: 1
direction: direction/forward
top level: BBa_J133653
start: 296
end: 298
orientation: inline

BBa I14032 P(Lac) IQ
type: SequenceAnnotation
persistent identity: annotation2125651
display id: annotation2125651
version: 1
top level: BBa_J133653
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_J133653/annotation2125651/range2125651/1

https://synbiohub.org/public/igem/BBa_J133653/annotation2125651/range2125651/1
type: Range
persistent identity: range2125651
display id: range2125651
version: 1
direction: direction/forward
top level: BBa_J133653
start: 13
end: 49
orientation: inline

BBa_K1486024
type: ComponentDefinition
persistent identity: BBa K1486024
display id: BBa_K1486024
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Yeast kanamycin resistance
creator: EPFL iGem team 2014
created: 2014-09-25T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: To edit
mutable notes: To edit
mutable provenance: To edit
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: TEF terminator
sequence annotation: KanR
sequence annotation: TEF promoter
sequence: https://synbiohub.org/public/igem/BBa_K1486024_sequence/1

TEF terminator
type: SequenceAnnotation
persistent identity: annotation2416944
display id: annotation2416944
version: 1
top level: BBa_K1486024
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1486024/annotation2416944/range2416944/1

https://synbiohub.org/public/igem/BBa_K1486024/annotation2416944/range2416944/1
type: Range
persistent identity: range2416944
display id: range2416944
version: 1
direction: direction/forward
top level: BBa_K1486024
start: 1159
end: 1357
orientation: inline

KanR
type: SequenceAnnotation
persistent identity: annotation2416943
display id: annotation2416943
version: 1
top level: BBa_K1486024
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1486024/annotation2416943/range2416943/1

https://synbiohub.org/public/igem/BBa_K1486024/annotation2416943/range2416943/1
type: Range
persistent identity: range2416943
display id: range2416943
version: 1
direction: direction/forward
top level: BBa_K1486024
start: 345
end: 1154
orientation: inline

TEF promoter
type: SequenceAnnotation
persistent identity: annotation2416942
display id: annotation2416942
version: 1
top level: BBa_K1486024
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1486024/annotation2416942/range2416942/1

https://synbiohub.org/public/igem/BBa_K1486024/annotation2416942/range2416942/1
type: Range
persistent identity: range2416942
display id: range2416942
version: 1
direction: direction/forward
top level: BBa_K1486024
start: 1
end: 344
orientation: inline

BBa_K1486027
type: ComponentDefinition
persistent identity: BBa K1486027
display id: BBa_K1486027
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: R.reniformis luciferase + ADH1 terminator + Kanamycin resistance
creator: EPFL iGem team 2014
created: 2014-09-26T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: To edit
mutable notes: To edit
mutable provenance: To edit
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000324
role: partType/Tag
component: BBa_K1486037
component: BBa_K1486019
component: BBa_K1486020
component: BBa_K801012
component: BBa_K1486024
sequence annotation: BBa_K1486024
sequence annotation: BBa_K1486020
sequence annotation: BBa_K801012
sequence annotation: BBa_K1486019
sequence annotation: BBa_K1486037
sequence: https://synbiohub.org/public/igem/BBa_K1486027_sequence/1

BBa_K1486037
type: Component
persistent identity: component2417706
display id: component2417706
version: 1
top level: BBa_K1486027
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486037/1

BBa_K1486019
type: Component
persistent identity: component2417707
display id: component2417707
version: 1
top level: BBa_K1486027
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486019/1

BBa_K1486020
type: Component
persistent identity: component2417708
display id: component2417708
version: 1
top level: BBa_K1486027
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486020/1

BBa_K801012
type: Component
persistent identity: component2417710
display id: component2417710
version: 1
top level: BBa_K1486027
access: public
definition: https://synbiohub.org/public/igem/BBa_K801012/1

BBa_K1486024
type: Component
persistent identity: component2417714
display id: component2417714
version: 1
top level: BBa_K1486027
access: public
definition: BBa_K1486024

BBa_K1486024
type: SequenceAnnotation
persistent identity: annotation2417714
display id: annotation2417714
version: 1
top level: BBa_K1486027
location: https://synbiohub.org/public/igem/BBa_K1486027/annotation2417714/range2417714/1
component: BBa_K1486024

https://synbiohub.org/public/igem/BBa_K1486027/annotation2417714/range2417714/1
type: Range
persistent identity: range2417714
display id: range2417714
version: 1
direction: direction/reverse
top level: BBa_K1486027
start: 1325
end: 2681
orientation: inline

BBa_K1486020
type: SequenceAnnotation
persistent identity: annotation2417708
display id: annotation2417708
version: 1
top level: BBa_K1486027
location: https://synbiohub.org/public/igem/BBa_K1486027/annotation2417708/range2417708/1
component: BBa_K1486020

https://synbiohub.org/public/igem/BBa_K1486027/annotation2417708/range2417708/1
type: Range
persistent identity: range2417708
display id: range2417708
version: 1
direction: direction/reverse
top level: BBa_K1486027
start: 370
end: 975
orientation: inline

BBa_K801012
type: SequenceAnnotation
persistent identity: annotation2417710
display id: annotation2417710
version: 1
top level: BBa_K1486027
location: https://synbiohub.org/public/igem/BBa_K1486027/annotation2417710/range2417710/1
component: BBa_K801012

https://synbiohub.org/public/igem/BBa_K1486027/annotation2417710/range2417710/1
type: Range
persistent identity: range2417710
display id: range2417710
version: 1
direction: direction/reverse
top level: BBa_K1486027
start: 976
end: 1324
orientation: inline

BBa_K1486019
type: SequenceAnnotation
persistent identity: annotation2417707
display id: annotation2417707
version: 1
top level: BBa_K1486027
location: https://synbiohub.org/public/igem/BBa_K1486027/annotation2417707/range2417707/1
component: BBa_K1486019

https://synbiohub.org/public/igem/BBa_K1486027/annotation2417707/range2417707/1
type: Range
persistent identity: range2417707
display id: range2417707
version: 1
direction: direction/reverse
top level: BBa_K1486027
start: 40
end: 369
orientation: inline

BBa_K1486037
type: SequenceAnnotation
persistent identity: annotation2417706
display id: annotation2417706
version: 1
top level: BBa_K1486027
location: https://synbiohub.org/public/igem/BBa_K1486027/annotation2417706/range2417706/1
component: BBa_K1486037

https://synbiohub.org/public/igem/BBa_K1486027/annotation2417706/range2417706/1
type: Range
persistent identity: range2417706
display id: range2417706
version: 1
direction: direction/reverse
top level: BBa_K1486027
start: 1
end: 39
orientation: inline

ble
type: ComponentDefinition
persistent identity: BBa K1033990
display id: BBa_K1033990
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Zeocin resistance cassette
creator: Erik Gullberg
created: 2013-09-02T11:00:00Z
modified: 2015-05-08T01:08:50Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1340_
m user id: 0
owner id: 10137
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: Zeocoin and bleomycin resistance cassette including promoter and coding sequence. It lacks a terminator.
mutable notes: Codon optimized for E coli by GenScript.
mutable provenance: Streptoalloteichus hindustanus, PDB: 1BYL
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: RBS
sequence annotation: Promoter
sequence annotation: ble
sequence: https://synbiohub.org/public/igem/BBa_K1033990_sequence/1

RBS
type: SequenceAnnotation
persistent identity: annotation2334638
display id: annotation2334638
version: 1
top level: ble
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1033990/annotation2334638/range2334638/1

https://synbiohub.org/public/igem/BBa_K1033990/annotation2334638/range2334638/1
type: Range
persistent identity: range2334638
display id: range2334638
version: 1
direction: direction/forward
top level: ble
start: 58
end: 64
orientation: inline

Promoter
type: SequenceAnnotation
persistent identity: annotation2334637
display id: annotation2334637
version: 1
top level: ble
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1033990/annotation2334637/range2334637/1

https://synbiohub.org/public/igem/BBa_K1033990/annotation2334637/range2334637/1
type: Range
persistent identity: range2334637
display id: range2334637
version: 1
direction: direction/forward
top level: ble
start: 2
end: 45
orientation: inline

ble
type: SequenceAnnotation
persistent identity: annotation2334639
display id: annotation2334639
version: 1
top level: ble
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1033990/annotation2334639/range2334639/1

https://synbiohub.org/public/igem/BBa_K1033990/annotation2334639/range2334639/1
type: Range
persistent identity: range2334639
display id: range2334639
version: 1
direction: direction/forward
top level: ble
start: 72
end: 446
orientation: inline

Aad9
type: ComponentDefinition
persistent identity: BBa K143031
display id: BBa_K143031
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Aad9 Spectinomycin Resistance Gene
creator: Chris Hirst
created: 2008-09-15T11:00:00Z
modified: 2015-05-08T01:10:24Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _199_
m user id: 0
owner id: 3475
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Aad9 is the spectinomycin resistance gene from ''Enterococcus faecalis''#1. Expression in a host confers resistance to spectinomycin at a concentration of 100μg/μl and has been used in a variety of vectors for both ''B. subtilis'' and ''E. coli'' including pDP870#2, pCOMT-Kan#3 and pIEF16s#4 ====References==== <biblio> #1 pmid=1659306 #2 pmid=16997985 #3 pmid=15060042 #4 pmid=16714443 </biblio>
mutable notes: The sequence of ''B. subtilis'' integration vector pDR111 was searched for the spectinomycin resistance gene and the Biobrick standard applied to the gene sequence upon PCR cloning
mutable provenance: Aad9 was PCR cloned from the ''B. subtilis'' integration vector pDR111 using Pfu DNA polymerase
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: stop
sequence annotation: start
sequence annotation: Aad9 Spectinomycin Acetyltransferase
sequence: https://synbiohub.org/public/igem/BBa_K143031_sequence/1

stop
type: SequenceAnnotation
persistent identity: annotation1992696
display id: annotation1992696
version: 1
top level: Aad9
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K143031/annotation1992696/range1992696/1

https://synbiohub.org/public/igem/BBa_K143031/annotation1992696/range1992696/1
type: Range
persistent identity: range1992696
display id: range1992696
version: 1
direction: direction/forward
top level: Aad9
start: 766
end: 768
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1992697
display id: annotation1992697
version: 1
top level: Aad9
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K143031/annotation1992697/range1992697/1

https://synbiohub.org/public/igem/BBa_K143031/annotation1992697/range1992697/1
type: Range
persistent identity: range1992697
display id: range1992697
version: 1
direction: direction/forward
top level: Aad9
start: 769
end: 771
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation1992698
display id: annotation1992698
version: 1
top level: Aad9
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K143031/annotation1992698/range1992698/1

https://synbiohub.org/public/igem/BBa_K143031/annotation1992698/range1992698/1
type: Range
persistent identity: range1992698
display id: range1992698
version: 1
direction: direction/forward
top level: Aad9
start: 1
end: 3
orientation: inline

Aad9 Spectinomycin Acetyltransferase
type: SequenceAnnotation
persistent identity: annotation1975961
display id: annotation1975961
version: 1
top level: Aad9
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K143031/annotation1975961/range1975961/1

https://synbiohub.org/public/igem/BBa_K143031/annotation1975961/range1975961/1
type: Range
persistent identity: range1975961
display id: range1975961
version: 1
direction: direction/forward
top level: Aad9
start: 1
end: 765
orientation: inline

NeoR
type: ComponentDefinition
persistent identity: BBa K1313004
display id: BBa_K1313004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Neomycin Resistance
creator: Carlos Meza
created: 2014-10-11T11:00:00Z
modified: 2016-01-28T01:43:08Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1688_
m user id: 4206
owner id: 19941
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: Neomycin resistance gene. This gene can be used for selection of cells with an efficient transformation. This gene codes neomycin phosphotransferase which breaks neomycin, kanamycin, and gentamicin molecules. If you use these gene as selectivity gene in bacteria, and your laboratory lacks neomycin, you can replace it for kanamycin by adding 35 mg/ml per liter of LB media. The best concentration of neomycin to use is 80 mg/ml per liter of LB media. This gene can be also used as a selective gene for mammalian cells using gentamicin.
mutable notes: Pst1 site cuts gene.
mutable provenance: This gene was obtained from pCDNA 3.1 myc His(A).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1313004_sequence/1

BBa_K1323013
type: ComponentDefinition
persistent identity: BBa K1323013
display id: BBa_K1323013
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: aadD - Kanamycin Resistance Gene Coding Sequence
creator: Julia Manalil
created: 2014-10-03T11:00:00Z
modified: 2016-01-28T01:43:33Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1698_
m user id: 4206
owner id: 18368
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: TBA
mutable notes: TBA
mutable provenance: TBA
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: aadD
sequence: https://synbiohub.org/public/igem/BBa_K1323013_sequence/1

aadD
type: SequenceAnnotation
persistent identity: annotation2428783
display id: annotation2428783
version: 1
top level: BBa_K1323013
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1323013/annotation2428783/range2428783/1

https://synbiohub.org/public/igem/BBa_K1323013/annotation2428783/range2428783/1
type: Range
persistent identity: range2428783
display id: range2428783
version: 1
direction: direction/forward
top level: BBa_K1323013
start: 1
end: 726
orientation: inline

CiproR
type: ComponentDefinition
persistent identity: BBa K1930004
display id: BBa_K1930004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Ciprofloxacin resistance cassette
creator: Mareike Schmidt
created: 2016-10-15T11:00:00Z
modified: 2016-10-16T04:58:30Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2397_
m user id: 29748
owner id: 29748
owning group id: 9
status: status/Planning
bookmark: true
mutable description: QnrS1 resistance cassette BioBrick BBa_K1930004.
mutable notes: To design a QnrS1 resistance cassette BioBrick BBa_K1930004 we designed a gBlock that contains the Bacillus subtilis promoter PAtpI, which is active from a very early stage of germination and includes a ribosome binding site. The gBlock also contains the original QnrS1 gene sequence from E. coli, the double terminator BBa_B0015 from iGEM as well as the prefix and suffix for BioBricks. In summary the ciprofloxacin cassette consists of the following parts PAtpI+RBS+QnrS1+2TER.
mutable provenance: Ordered as gBlock. IDT (Integrated DNA technologies).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: Qnrs1
sequence annotation: BBa_B0015
sequence annotation: BBa_K1930005
sequence annotation: 2TER
sequence annotation: P(AtpI)
sequence: https://synbiohub.org/public/igem/BBa_K1930004_sequence/1

Qnrs1
type: SequenceAnnotation
persistent identity: annotation2513504
display id: annotation2513504
version: 1
top level: CiproR
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1930004/annotation2513504/range2513504/1

https://synbiohub.org/public/igem/BBa_K1930004/annotation2513504/range2513504/1
type: Range
persistent identity: range2513504
display id: range2513504
version: 1
direction: direction/forward
top level: CiproR
start: 351
end: 1007
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2513506
display id: annotation2513506
version: 1
top level: CiproR
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1930004/annotation2513506/range2513506/1

https://synbiohub.org/public/igem/BBa_K1930004/annotation2513506/range2513506/1
type: Range
persistent identity: range2513506
display id: range2513506
version: 1
direction: direction/forward
top level: CiproR
start: 1008
end: 1136
orientation: inline

BBa_K1930005
type: SequenceAnnotation
persistent identity: annotation2513503
display id: annotation2513503
version: 1
top level: CiproR
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1930004/annotation2513503/range2513503/1

https://synbiohub.org/public/igem/BBa_K1930004/annotation2513503/range2513503/1
type: Range
persistent identity: range2513503
display id: range2513503
version: 1
direction: direction/forward
top level: CiproR
start: 1
end: 350
orientation: inline

2TER
type: SequenceAnnotation
persistent identity: annotation2513505
display id: annotation2513505
version: 1
top level: CiproR
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1930004/annotation2513505/range2513505/1

https://synbiohub.org/public/igem/BBa_K1930004/annotation2513505/range2513505/1
type: Range
persistent identity: range2513505
display id: range2513505
version: 1
direction: direction/forward
top level: CiproR
start: 1008
end: 1136
orientation: inline

P(AtpI)
type: SequenceAnnotation
persistent identity: annotation2513502
display id: annotation2513502
version: 1
top level: CiproR
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1930004/annotation2513502/range2513502/1

https://synbiohub.org/public/igem/BBa_K1930004/annotation2513502/range2513502/1
type: Range
persistent identity: range2513502
display id: range2513502
version: 1
direction: direction/forward
top level: CiproR
start: 1
end: 350
orientation: inline

Ori+Kan
type: ComponentDefinition
persistent identity: BBa K320004
display id: BBa_K320004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Origin for Asaia and resistance to Kanamycin
creator: Perrudet Christian
created: 2010-10-17T11:00:00Z
modified: 2015-05-08T01:11:59Z
discontinued: false
dominant: false
experience: experience/Issues
group u list: _439_
m user id: 0
owner id: 7149
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: t
mutable notes: t
mutable provenance: t
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: KanR
sequence annotation: BBa_K320000
sequence annotation: Asaia replication origin
sequence: https://synbiohub.org/public/igem/BBa_K320004_sequence/1

KanR
type: SequenceAnnotation
persistent identity: annotation2091398
display id: annotation2091398
version: 1
top level: Ori+Kan
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K320004/annotation2091398/range2091398/1

https://synbiohub.org/public/igem/BBa_K320004/annotation2091398/range2091398/1
type: Range
persistent identity: range2091398
display id: range2091398
version: 1
direction: direction/forward
top level: Ori+Kan
start: 1400
end: 2372
orientation: inline

BBa_K320000
type: SequenceAnnotation
persistent identity: annotation2088717
display id: annotation2088717
version: 1
top level: Ori+Kan
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K320004/annotation2088717/range2088717/1

https://synbiohub.org/public/igem/BBa_K320004/annotation2088717/range2088717/1
type: Range
persistent identity: range2088717
display id: range2088717
version: 1
direction: direction/reverse
top level: Ori+Kan
start: 1
end: 1399
orientation: inline

Asaia replication origin
type: SequenceAnnotation
persistent identity: annotation2091397
display id: annotation2091397
version: 1
top level: Ori+Kan
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K320004/annotation2091397/range2091397/1

https://synbiohub.org/public/igem/BBa_K320004/annotation2091397/range2091397/1
type: Range
persistent identity: range2091397
display id: range2091397
version: 1
direction: direction/forward
top level: Ori+Kan
start: 355
end: 1029
orientation: inline

hygR
type: ComponentDefinition
persistent identity: BBa K678021
display id: BBa_K678021
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Hygromycin cassette
creator: DTU-Denmark-2
created: 2011-09-03T11:00:00Z
modified: 2015-05-08T01:13:04Z
discontinued: false
dominant: false
experience: experience/None
group u list: _882_
m user id: 0
owner id: 8341
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Hygromycin cassette for selection of mammalian cells. The selection cassette contains the SV40 promoter, the hygromycin resistance gene, and the SV40 poly A as terminator
mutable notes: Compatible with the Plu'n'Play assembly standard
mutable provenance: From plasmid pcDNA3.1/Hygro from Invitrogen
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Other
role: SO:0000110
sequence annotation: Linker 5
sequence annotation: Hygromycin cassette
sequence: https://synbiohub.org/public/igem/BBa_K678021_sequence/1

Linker 5
type: SequenceAnnotation
persistent identity: annotation2127356
display id: annotation2127356
version: 1
top level: hygR
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K678021/annotation2127356/range2127356/1

https://synbiohub.org/public/igem/BBa_K678021/annotation2127356/range2127356/1
type: Range
persistent identity: range2127356
display id: range2127356
version: 1
direction: direction/forward
top level: hygR
start: 1
end: 8
orientation: inline

Hygromycin cassette
type: SequenceAnnotation
persistent identity: annotation2127357
display id: annotation2127357
version: 1
top level: hygR
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K678021/annotation2127357/range2127357/1

https://synbiohub.org/public/igem/BBa_K678021/annotation2127357/range2127357/1
type: Range
persistent identity: range2127357
display id: range2127357
version: 1
direction: direction/forward
top level: hygR
start: 9
end: 1759
orientation: inline

BBa_K1015002
type: ComponentDefinition
persistent identity: BBa K1015002
display id: BBa_K1015002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: streptmycin resisitance gene (can PCR ampicillin resistance gene primer)
creator: Kento Tominaga
created: 2013-09-14T11:00:00Z
modified: 2016-01-28T01:50:11Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1321_
m user id: 4206
owner id: 15618
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: streptmycin resisitance gene(aadA) This aadA gene are inserted into blaZ gene come from pI258 plasmid. So this aadA gene can PCR with primers for blaZ.
mutable notes: antibiotic resistance
mutable provenance: aminoglycoside resistance gene (aadA) come from R100.1 plasmid.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: blaZ fragment(promoter contain)
sequence annotation: aadA(streptomycin resistance gene)
sequence annotation: blaZ fragment
sequence: https://synbiohub.org/public/igem/BBa_K1015002_sequence/1

blaZ fragment(promoter contain)
type: SequenceAnnotation
persistent identity: annotation2342881
display id: annotation2342881
version: 1
top level: BBa_K1015002
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1015002/annotation2342881/range2342881/1

https://synbiohub.org/public/igem/BBa_K1015002/annotation2342881/range2342881/1
type: Range
persistent identity: range2342881
display id: range2342881
version: 1
direction: direction/forward
top level: BBa_K1015002
start: 1
end: 118
orientation: inline

aadA(streptomycin resistance gene)
type: SequenceAnnotation
persistent identity: annotation2342880
display id: annotation2342880
version: 1
top level: BBa_K1015002
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1015002/annotation2342880/range2342880/1

https://synbiohub.org/public/igem/BBa_K1015002/annotation2342880/range2342880/1
type: Range
persistent identity: range2342880
display id: range2342880
version: 1
direction: direction/forward
top level: BBa_K1015002
start: 119
end: 911
orientation: inline

blaZ fragment
type: SequenceAnnotation
persistent identity: annotation2342888
display id: annotation2342888
version: 1
top level: BBa_K1015002
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1015002/annotation2342888/range2342888/1

https://synbiohub.org/public/igem/BBa_K1015002/annotation2342888/range2342888/1
type: Range
persistent identity: range2342888
display id: range2342888
version: 1
direction: direction/forward
top level: BBa_K1015002
start: 911
end: 1030
orientation: inline

BBa_K1486035
type: ComponentDefinition
persistent identity: BBa K1486035
display id: BBa_K1486035
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Yeast Optimized sfGFP-C + ADH1 terminator + CaUra3 Cassette
creator: EPFL iGem team 2014
created: 2014-09-26T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: To edit
mutable notes: To edit
mutable provenance: To edit
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000324
role: partType/Tag
component: BBa_K1486034
component: BBa_K1486037
component: BBa_K1486031
component: BBa_K1486025
sequence annotation: BBa_K1486025
sequence annotation: BBa_K1486031
sequence annotation: BBa_K1486034
sequence annotation: BBa_K1486037
sequence: https://synbiohub.org/public/igem/BBa_K1486035_sequence/1

BBa_K1486034
type: Component
persistent identity: component2418330
display id: component2418330
version: 1
top level: BBa_K1486035
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486034/1

BBa_K1486037
type: Component
persistent identity: component2418328
display id: component2418328
version: 1
top level: BBa_K1486035
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486037/1

BBa_K1486031
type: Component
persistent identity: component2418335
display id: component2418335
version: 1
top level: BBa_K1486035
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486031/1

BBa_K1486025
type: Component
persistent identity: component2418331
display id: component2418331
version: 1
top level: BBa_K1486035
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486025/1

BBa_K1486025
type: SequenceAnnotation
persistent identity: annotation2418331
display id: annotation2418331
version: 1
top level: BBa_K1486035
location: https://synbiohub.org/public/igem/BBa_K1486035/annotation2418331/range2418331/1
component: BBa_K1486025

https://synbiohub.org/public/igem/BBa_K1486035/annotation2418331/range2418331/1
type: Range
persistent identity: range2418331
display id: range2418331
version: 1
direction: direction/reverse
top level: BBa_K1486035
start: 115
end: 302
orientation: inline

BBa_K1486031
type: SequenceAnnotation
persistent identity: annotation2418335
display id: annotation2418335
version: 1
top level: BBa_K1486035
location: https://synbiohub.org/public/igem/BBa_K1486035/annotation2418335/range2418335/1
component: BBa_K1486031

https://synbiohub.org/public/igem/BBa_K1486035/annotation2418335/range2418335/1
type: Range
persistent identity: range2418335
display id: range2418335
version: 1
direction: direction/reverse
top level: BBa_K1486035
start: 303
end: 1662
orientation: inline

BBa_K1486034
type: SequenceAnnotation
persistent identity: annotation2418330
display id: annotation2418330
version: 1
top level: BBa_K1486035
location: https://synbiohub.org/public/igem/BBa_K1486035/annotation2418330/range2418330/1
component: BBa_K1486034

https://synbiohub.org/public/igem/BBa_K1486035/annotation2418330/range2418330/1
type: Range
persistent identity: range2418330
display id: range2418330
version: 1
direction: direction/reverse
top level: BBa_K1486035
start: 40
end: 114
orientation: inline

BBa_K1486037
type: SequenceAnnotation
persistent identity: annotation2418328
display id: annotation2418328
version: 1
top level: BBa_K1486035
location: https://synbiohub.org/public/igem/BBa_K1486035/annotation2418328/range2418328/1
component: BBa_K1486037

https://synbiohub.org/public/igem/BBa_K1486035/annotation2418328/range2418328/1
type: Range
persistent identity: range2418328
display id: range2418328
version: 1
direction: direction/reverse
top level: BBa_K1486035
start: 1
end: 39
orientation: inline

BBa_K1071001
type: ComponentDefinition
persistent identity: BBa K1071001
display id: BBa_K1071001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: ble resistance (eucaryotic)
creator: Team Marburg 2013
created: 2013-09-18T11:00:00Z
modified: 2016-01-28T01:51:30Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1380_
m user id: 4206
owner id: 18233
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: Gives resistance against the antibiotic zeocin for eucaryotes.
mutable notes: No mutations added
mutable provenance: Phaeodactylum tricornutum
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1071001_sequence/1

tetA(C)b
type: ComponentDefinition
persistent identity: BBa J31006
display id: BBa_J31006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: tetracycline resistance protein TetA(C) (backwards) [cf. BBa_J31007]
creator: Sabriya Rosemond, Erin Zwack
created: 2006-07-11T11:00:00Z
modified: 2015-08-31T04:08:45Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _61_
m user id: 0
owner id: 919
owning group id: 61
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This is the coding region for Tetracyline resistance in the reverse orientation. If a promoter adn RBS are placed downstream and the entire region, from promoter to stop codon is flipped the cell will express tetracycline resistance.
mutable notes: It was cloned into PsB1A2 plasmids.
mutable provenance: PsB1AT3
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: stop
sequence annotation: Rev primer J31006
sequence annotation: Fwd primer J31006
sequence annotation: tetA(C) backwards
sequence: https://synbiohub.org/public/igem/BBa_J31006_sequence/1

stop
type: SequenceAnnotation
persistent identity: annotation1910574
display id: annotation1910574
version: 1
top level: tetA(C)b
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_J31006/annotation1910574/range1910574/1

https://synbiohub.org/public/igem/BBa_J31006/annotation1910574/range1910574/1
type: Range
persistent identity: range1910574
display id: range1910574
version: 1
direction: direction/reverse
top level: tetA(C)b
start: 1
end: 3
orientation: inline

Rev primer J31006
type: SequenceAnnotation
persistent identity: annotation1910575
display id: annotation1910575
version: 1
top level: tetA(C)b
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_J31006/annotation1910575/range1910575/1

https://synbiohub.org/public/igem/BBa_J31006/annotation1910575/range1910575/1
type: Range
persistent identity: range1910575
display id: range1910575
version: 1
direction: direction/forward
top level: tetA(C)b
start: 1
end: 20
orientation: inline

Fwd primer J31006
type: SequenceAnnotation
persistent identity: annotation1910576
display id: annotation1910576
version: 1
top level: tetA(C)b
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_J31006/annotation1910576/range1910576/1

https://synbiohub.org/public/igem/BBa_J31006/annotation1910576/range1910576/1
type: Range
persistent identity: range1910576
display id: range1910576
version: 1
direction: direction/reverse
top level: tetA(C)b
start: 1168
end: 1191
orientation: inline

tetA(C) backwards
type: SequenceAnnotation
persistent identity: annotation1884998
display id: annotation1884998
version: 1
top level: tetA(C)b
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J31006/annotation1884998/range1884998/1

https://synbiohub.org/public/igem/BBa_J31006/annotation1884998/range1884998/1
type: Range
persistent identity: range1884998
display id: range1884998
version: 1
direction: direction/reverse
top level: tetA(C)b
start: 1
end: 1191
orientation: inline

ptrA
type: ComponentDefinition
persistent identity: BBa K678045
display id: BBa_K678045
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: ptrA, selection marker for Aspergillus nidulans
creator: DTU-Denmark-2
created: 2011-09-04T11:00:00Z
modified: 2015-05-08T01:13:04Z
discontinued: false
dominant: false
experience: experience/None
group u list: _882_
m user id: 0
owner id: 8341
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: ptrA, selection marker for Aspergillus nidulans
mutable notes: Compatible with the Plug'n'Play assembly standard
mutable provenance: ptrA
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: ptrA cassette
sequence annotation: Linker 5
sequence: https://synbiohub.org/public/igem/BBa_K678045_sequence/1

ptrA cassette
type: SequenceAnnotation
persistent identity: annotation2127422
display id: annotation2127422
version: 1
top level: ptrA
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K678045/annotation2127422/range2127422/1

https://synbiohub.org/public/igem/BBa_K678045/annotation2127422/range2127422/1
type: Range
persistent identity: range2127422
display id: range2127422
version: 1
direction: direction/forward
top level: ptrA
start: 9
end: 1899
orientation: inline

Linker 5
type: SequenceAnnotation
persistent identity: annotation2127421
display id: annotation2127421
version: 1
top level: ptrA
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K678045/annotation2127421/range2127421/1

https://synbiohub.org/public/igem/BBa_K678045/annotation2127421/range2127421/1
type: Range
persistent identity: range2127421
display id: range2127421
version: 1
direction: direction/forward
top level: ptrA
start: 1
end: 8
orientation: inline

BBa_K1323012
type: ComponentDefinition
persistent identity: BBa K1323012
display id: BBa_K1323012
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Spc - Spectinomycin Resistance Gene Coding Sequence
creator: Julia Manalil
created: 2014-10-03T11:00:00Z
modified: 2016-01-28T01:43:19Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1698_
m user id: 4206
owner id: 18368
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: TBA
mutable notes: TBA
mutable provenance: TBA
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: spc
sequence: https://synbiohub.org/public/igem/BBa_K1323012_sequence/1

spc
type: SequenceAnnotation
persistent identity: annotation2428780
display id: annotation2428780
version: 1
top level: BBa_K1323012
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1323012/annotation2428780/range2428780/1

https://synbiohub.org/public/igem/BBa_K1323012/annotation2428780/range2428780/1
type: Range
persistent identity: range2428780
display id: range2428780
version: 1
direction: direction/forward
top level: BBa_K1323012
start: 1
end: 783
orientation: inline

BBa_K823020
type: ComponentDefinition
persistent identity: BBa K823020
display id: BBa_K823020
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: chloramphenicol acetyltransferase (cat)
creator: Simon Kelterborn
created: 2012-08-14T11:00:00Z
modified: 2015-05-08T01:13:30Z
discontinued: false
dominant: false
group u list: _1081_
m user id: 0
owner id: 11555
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part codes for the chloramphenicol acetyltransferase (cat) gene used in Bacillus subtilis for resistance against the antibiotic chloramphenicol. We constructed it in the freiburg standard (Assembly 25) for in-frame fusion-proteins. There is a Shine-Dalgarno Sequence (RBS) optimized for Bacillus subtilis included between the XbaI and NgoIV restriction site. You can use it both N-and C terminal fusions.
mutable notes: We constructed the part in the freiburg standard (Assembly 25). We included a Bacillus optimized RBS between the XbaI and NgoIV restriction site.
mutable provenance: Plasmid: Bacillus subtilis reporter vector pAC6
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: cat
sequence: https://synbiohub.org/public/igem/BBa_K823020_sequence/1

cat
type: SequenceAnnotation
persistent identity: annotation2179570
display id: annotation2179570
version: 1
top level: BBa_K823020
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K823020/annotation2179570/range2179570/1

https://synbiohub.org/public/igem/BBa_K823020/annotation2179570/range2179570/1
type: Range
persistent identity: range2179570
display id: range2179570
version: 1
direction: direction/forward
top level: BBa_K823020
start: 1
end: 645
orientation: inline

BBa_K1930002
type: ComponentDefinition
persistent identity: BBa K1930002
display id: BBa_K1930002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Encryption key sequence in integration plasmid (BBa_K823023)
creator: Eike Mahlandt
created: 2016-10-15T11:00:00Z
modified: 2016-10-17T09:00:28Z
discontinued: true
dominant: false
experience: experience/Works
group u list: _2397_
m user id: 29748
owner id: 29748
owning group id: 9
status: status/Deleted
bookmark: false
mutable description: The iGEM team Groningen 2016 worked on bioencryption in order to safely store data in DNA. In our project we were mainly working with two sequences of DNA which were the encrypted message sequence and the encryption key sequence. The key sequence was created by our software. It is therefore artificial DNA. We ordered the encrypted key sequence as gBlock from IDT (Integrated DNA technologies). This part was created in the interest of having a genomic integration plasmid with our key sequence. We used an available BioBrick BBa_K823023 from igem Munich 2012. It is an integration plasmid for Bacillus subtilis, which can be used for cloning in E. coli as well. An RFP is inserted in BBa_K823023 for more efficient screening after transformation.
mutable notes: The iGEM team Groningen 2016 worked on bioencryption in order to safely store data in DNA. In our project we were mainly working with two sequences of DNA which were the encrypted message sequence and the encryption key sequence. The key sequence was created by our software. It is therefore artificial DNA. We ordered the encrypted key sequence as gBlock from IDT (Integrated DNA technologies). This part was created in the interest of having a genomic integration plasmid with our key sequence. We used an available BioBrick BBa_K823023 from igem Munich 2012. It is an integration plasmid for Bacillus subtilis, which can be used for cloning in E. coli as well. An RFP is inserted in BBa_K823023 for more efficient screening after transformation.
mutable provenance: Key sequence: Created by our CryptoGErM software. http://2016.igem.org/Team:Groningen/Encoding BBa_K823023: Created by iGEM Munich 2012. http://parts.igem.org/Part:BBa_K823023
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000155
role: partType/Plasmid
sequence annotation: key sequence
sequence annotation: cat
sequence annotation: BBa_K1930000
sequence annotation: amyE
sequence annotation: bla
sequence annotation: BBa_K823023
sequence: https://synbiohub.org/public/igem/BBa_K1930002_sequence/1

key sequence
type: SequenceAnnotation
persistent identity: annotation2514195
display id: annotation2514195
version: 1
top level: BBa_K1930002
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1930002/annotation2514195/range2514195/1

https://synbiohub.org/public/igem/BBa_K1930002/annotation2514195/range2514195/1
type: Range
persistent identity: range2514195
display id: range2514195
version: 1
direction: direction/forward
top level: BBa_K1930002
start: 6063
end: 6206
orientation: inline

cat
type: SequenceAnnotation
persistent identity: annotation2514191
display id: annotation2514191
version: 1
top level: BBa_K1930002
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1930002/annotation2514191/range2514191/1

https://synbiohub.org/public/igem/BBa_K1930002/annotation2514191/range2514191/1
type: Range
persistent identity: range2514191
display id: range2514191
version: 1
direction: direction/forward
top level: BBa_K1930002
start: 337
end: 987
orientation: inline

BBa_K1930000
type: SequenceAnnotation
persistent identity: annotation2514196
display id: annotation2514196
version: 1
top level: BBa_K1930002
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1930002/annotation2514196/range2514196/1

https://synbiohub.org/public/igem/BBa_K1930002/annotation2514196/range2514196/1
type: Range
persistent identity: range2514196
display id: range2514196
version: 1
direction: direction/forward
top level: BBa_K1930002
start: 6063
end: 6206
orientation: inline

amyE
type: SequenceAnnotation
persistent identity: annotation2514194
display id: annotation2514194
version: 1
top level: BBa_K1930002
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1930002/annotation2514194/range2514194/1

https://synbiohub.org/public/igem/BBa_K1930002/annotation2514194/range2514194/1
type: Range
persistent identity: range2514194
display id: range2514194
version: 1
direction: direction/forward
top level: BBa_K1930002
start: 5525
end: 6045
orientation: inline

bla
type: SequenceAnnotation
persistent identity: annotation2514193
display id: annotation2514193
version: 1
top level: BBa_K1930002
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1930002/annotation2514193/range2514193/1

https://synbiohub.org/public/igem/BBa_K1930002/annotation2514193/range2514193/1
type: Range
persistent identity: range2514193
display id: range2514193
version: 1
direction: direction/forward
top level: BBa_K1930002
start: 4340
end: 5200
orientation: inline

amyE
type: SequenceAnnotation
persistent identity: annotation2514192
display id: annotation2514192
version: 1
top level: BBa_K1930002
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1930002/annotation2514192/range2514192/1

https://synbiohub.org/public/igem/BBa_K1930002/annotation2514192/range2514192/1
type: Range
persistent identity: range2514192
display id: range2514192
version: 1
direction: direction/forward
top level: BBa_K1930002
start: 1358
end: 2386
orientation: inline

BBa_K823023
type: SequenceAnnotation
persistent identity: annotation2514190
display id: annotation2514190
version: 1
top level: BBa_K1930002
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1930002/annotation2514190/range2514190/1

https://synbiohub.org/public/igem/BBa_K1930002/annotation2514190/range2514190/1
type: Range
persistent identity: range2514190
display id: range2514190
version: 1
direction: direction/forward
top level: BBa_K1930002
start: 1
end: 6062
orientation: inline

BBa_T9010
type: ComponentDefinition
persistent identity: BBa T9010
display id: BBa_T9010
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: aacC2 with RBS
creator: Abigail Sison
created: 2016-08-21T11:00:00Z
modified: 2016-08-22T11:02:40Z
discontinued: false
dominant: false
group u list: _1_
m user id: 33565
owner id: 33565
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Translational unit including aacC2 gene encoding gentamicin resistance (BBa_T9000) with strong RBS BBa_B0034
mutable notes: Changed TAG stop codon to TAA
mutable provenance: Escherichia coli R-Plasmid. Sequence from Genbank: http://www.ncbi.nlm.nih.gov/nuccore/X54723
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_B0034
component: BBa_T9000
sequence annotation: BBa_T9000
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_T9010_sequence/1

BBa_B0034
type: Component
persistent identity: component2481504
display id: component2481504
version: 1
top level: BBa_T9010
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_T9000
type: Component
persistent identity: component2481508
display id: component2481508
version: 1
top level: BBa_T9010
access: public
definition: BBa_T9000

BBa_T9000
type: SequenceAnnotation
persistent identity: annotation2481508
display id: annotation2481508
version: 1
top level: BBa_T9010
location: https://synbiohub.org/public/igem/BBa_T9010/annotation2481508/range2481508/1
component: BBa_T9000

https://synbiohub.org/public/igem/BBa_T9010/annotation2481508/range2481508/1
type: Range
persistent identity: range2481508
display id: range2481508
version: 1
direction: direction/reverse
top level: BBa_T9010
start: 19
end: 879
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2481504
display id: annotation2481504
version: 1
top level: BBa_T9010
location: https://synbiohub.org/public/igem/BBa_T9010/annotation2481504/range2481504/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_T9010/annotation2481504/range2481504/1
type: Range
persistent identity: range2481504
display id: range2481504
version: 1
direction: direction/reverse
top level: BBa_T9010
start: 1
end: 12
orientation: inline

BBa_K678047
type: ComponentDefinition
persistent identity: BBa K678047
display id: BBa_K678047
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Ampicillin cassette
creator: DTU-Denmark-2
created: 2011-09-06T11:00:00Z
modified: 2015-05-08T01:13:04Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _882_
m user id: 0
owner id: 8341
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: For selection of E. coli transformants, contains promoter, ampR gene, and terminator
mutable notes: Compatible with the Plug'n'Play assembly standard
mutable provenance: From plasmid pcDNA3.1 from Invitrogen
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
sequence annotation: ampR cassette
sequence annotation: Linker 5
sequence: https://synbiohub.org/public/igem/BBa_K678047_sequence/1

ampR cassette
type: SequenceAnnotation
persistent identity: annotation2127426
display id: annotation2127426
version: 1
top level: BBa_K678047
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K678047/annotation2127426/range2127426/1

https://synbiohub.org/public/igem/BBa_K678047/annotation2127426/range2127426/1
type: Range
persistent identity: range2127426
display id: range2127426
version: 1
direction: direction/forward
top level: BBa_K678047
start: 9
end: 867
orientation: inline

Linker 5
type: SequenceAnnotation
persistent identity: annotation2127425
display id: annotation2127425
version: 1
top level: BBa_K678047
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K678047/annotation2127425/range2127425/1

https://synbiohub.org/public/igem/BBa_K678047/annotation2127425/range2127425/1
type: Range
persistent identity: range2127425
display id: range2127425
version: 1
direction: direction/forward
top level: BBa_K678047
start: 1
end: 8
orientation: inline

ampR
type: ComponentDefinition
persistent identity: BBa K1073000
display id: BBa_K1073000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: ampicillin resistance
creator: iGEM Team Braunschweig 2013
created: 2013-09-15T11:00:00Z
modified: 2016-01-28T01:50:42Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1382_
m user id: 4206
owner id: 15674
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This sequence codes for a beta-lactamase, which degrades ampicillin. There is no RBS or other information attached, only the sequence for the enzyme plus prefix and suffix.
mutable notes: The primer sequences can be found on iGEM Team Braunschweig's wiki.
mutable provenance: The part was derived from BBa_P1002 ampicillin resistance cassette. The sequence for the beta-lactamase was gained by PCR of the original BBa_P1002 with appropiate primers.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1073000_sequence/1

BBa_K1015009
type: ComponentDefinition
persistent identity: BBa K1015009
display id: BBa_K1015009
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Chloramphenicol resistance gene+FRT
creator: Marina Ikeno
created: 2013-09-15T11:00:00Z
modified: 2016-01-28T01:50:53Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1321_
m user id: 4206
owner id: 13704
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Chloramphenicol resistance gene Chloramphenicol is effective against a wide variety of Gram-positive and Gram-negative bacteria, including most anaerobic organisms.This part includes a gene which has resistance to chloramphenicol. In our project, we use this part as a marker to select colonies of E.coli which succeeded in transformation.
mutable notes: selection marker
mutable provenance: Streptomyces coelicolor A3(2)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Cm
sequence annotation: FRT
sequence: https://synbiohub.org/public/igem/BBa_K1015009_sequence/1

Cm
type: SequenceAnnotation
persistent identity: annotation2347534
display id: annotation2347534
version: 1
top level: BBa_K1015009
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1015009/annotation2347534/range2347534/1

https://synbiohub.org/public/igem/BBa_K1015009/annotation2347534/range2347534/1
type: Range
persistent identity: range2347534
display id: range2347534
version: 1
direction: direction/forward
top level: BBa_K1015009
start: 395
end: 1055
orientation: inline

FRT
type: SequenceAnnotation
persistent identity: annotation2347569
display id: annotation2347569
version: 1
top level: BBa_K1015009
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1015009/annotation2347569/range2347569/1

https://synbiohub.org/public/igem/BBa_K1015009/annotation2347569/range2347569/1
type: Range
persistent identity: range2347569
display id: range2347569
version: 1
direction: direction/forward
top level: BBa_K1015009
start: 47
end: 80
orientation: inline

hygR
type: ComponentDefinition
persistent identity: BBa K678042
display id: BBa_K678042
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: hygromycin cassette for Aspergillus nidulans
creator: DTU-Denmark-2
created: 2011-09-04T11:00:00Z
modified: 2015-05-08T01:13:04Z
discontinued: false
dominant: false
experience: experience/None
group u list: _882_
m user id: 0
owner id: 8341
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: hygromycin cassette for Aspergillus nidulans
mutable notes: Compatible with the Plug'n'Play assembly standard
mutable provenance: pCB1001
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: hygR cassette
sequence annotation: Linker 5
sequence: https://synbiohub.org/public/igem/BBa_K678042_sequence/1

hygR cassette
type: SequenceAnnotation
persistent identity: annotation2127416
display id: annotation2127416
version: 1
top level: hygR
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K678042/annotation2127416/range2127416/1

https://synbiohub.org/public/igem/BBa_K678042/annotation2127416/range2127416/1
type: Range
persistent identity: range2127416
display id: range2127416
version: 1
direction: direction/forward
top level: hygR
start: 9
end: 1363
orientation: inline

Linker 5
type: SequenceAnnotation
persistent identity: annotation2127415
display id: annotation2127415
version: 1
top level: hygR
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K678042/annotation2127415/range2127415/1

https://synbiohub.org/public/igem/BBa_K678042/annotation2127415/range2127415/1
type: Range
persistent identity: range2127415
display id: range2127415
version: 1
direction: direction/forward
top level: hygR
start: 1
end: 8
orientation: inline

BBa_K1207001
type: ComponentDefinition
persistent identity: BBa K1207001
display id: BBa_K1207001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Macrolide-Lincosamide-Streptogramin B Resistance Marker
creator: Jeremiah Miller
created: 2013-09-11T11:00:00Z
modified: 2016-01-28T01:51:46Z
discontinued: false
dominant: false
group u list: _1521_1241_
m user id: 4206
owner id: 14319
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This mlsR gene was chosen as an additional selection marker for transformation of Gram positive chassis organisms. In the intended chassis organism of Clostridium acetobutylicum, the concentrations for selectivity were known, making this a convenient choice.
mutable notes: The cloning of this part was relatively straight-forward. No special design considerations were required.
mutable provenance: This part was cloned from the plasmid pIKM1.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1207001_sequence/1

BBa_J23012
type: ComponentDefinition
persistent identity: BBa J23012
display id: BBa_J23012
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Spectinomycin R open-reading-frame basic part
creator: Will Bosworth
created: 2006-07-23T11:00:00Z
modified: 2015-08-31T04:08:38Z
discontinued: false
dominant: false
experience: experience/Issues
group u list: _52_
m user id: 0
owner id: 363
owning group id: 52
sample status: In stock
status: status/Available
bookmark: false
mutable description: it is a Spec resistant biobrick
mutable notes: JL1 Forward EcoRI oligo to bioBrick SpecR: gacttgaattcgcggccgcttctagagtccaagcgagctcgatatc JL2 Reverse SpeI oligo to bioBrick SpecR: ccgctactagtCAGCAACGATGTTACGCAGC
mutable provenance: PCR JL1/JL2 on wt. pSPlam (917bp, EcoRI/SpeI, Neb2) Paste into pSB1A2-I13521 (EcoRI/SpeI, Neb2, Large 2062)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: SpR Spectinomycin Resistance
sequence: https://synbiohub.org/public/igem/BBa_J23012_sequence/1

SpR Spectinomycin Resistance
type: SequenceAnnotation
persistent identity: annotation1966465
display id: annotation1966465
version: 1
top level: BBa_J23012
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J23012/annotation1966465/range1966465/1

https://synbiohub.org/public/igem/BBa_J23012/annotation1966465/range1966465/1
type: Range
persistent identity: range1966465
display id: range1966465
version: 1
direction: direction/reverse
top level: BBa_J23012
start: 46
end: 837
orientation: inline

BBa_K1486026
type: ComponentDefinition
persistent identity: BBa K1486026
display id: BBa_K1486026
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: sfGFP + Kanamycin resistance for yeast
creator: EPFL iGem team 2014
created: 2014-09-25T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: To edit
mutable notes: To edit
mutable provenance: To edit
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000324
role: partType/Tag
sequence annotation: BBa_K1486023
sequence annotation: Adh1 terminator
sequence annotation: BBa_K1486024
sequence annotation: Pbs2 flanking
sequence annotation: 8 a.a linker
sequence: https://synbiohub.org/public/igem/BBa_K1486026_sequence/1

BBa_K1486023
type: SequenceAnnotation
persistent identity: annotation2417049
display id: annotation2417049
version: 1
top level: BBa_K1486026
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1486026/annotation2417049/range2417049/1

https://synbiohub.org/public/igem/BBa_K1486026/annotation2417049/range2417049/1
type: Range
persistent identity: range2417049
display id: range2417049
version: 1
direction: direction/forward
top level: BBa_K1486026
start: 75
end: 791
orientation: inline

Adh1 terminator
type: SequenceAnnotation
persistent identity: annotation2417050
display id: annotation2417050
version: 1
top level: BBa_K1486026
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1486026/annotation2417050/range2417050/1

https://synbiohub.org/public/igem/BBa_K1486026/annotation2417050/range2417050/1
type: Range
persistent identity: range2417050
display id: range2417050
version: 1
direction: direction/forward
top level: BBa_K1486026
start: 811
end: 999
orientation: inline

BBa_K1486024
type: SequenceAnnotation
persistent identity: annotation2417051
display id: annotation2417051
version: 1
top level: BBa_K1486026
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1486026/annotation2417051/range2417051/1

https://synbiohub.org/public/igem/BBa_K1486026/annotation2417051/range2417051/1
type: Range
persistent identity: range2417051
display id: range2417051
version: 1
direction: direction/forward
top level: BBa_K1486026
start: 1073
end: 2430
orientation: inline

Pbs2 flanking
type: SequenceAnnotation
persistent identity: annotation2417047
display id: annotation2417047
version: 1
top level: BBa_K1486026
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1486026/annotation2417047/range2417047/1

https://synbiohub.org/public/igem/BBa_K1486026/annotation2417047/range2417047/1
type: Range
persistent identity: range2417047
display id: range2417047
version: 1
direction: direction/forward
top level: BBa_K1486026
start: 1
end: 50
orientation: inline

Pbs2 flanking
type: SequenceAnnotation
persistent identity: annotation2417052
display id: annotation2417052
version: 1
top level: BBa_K1486026
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1486026/annotation2417052/range2417052/1

https://synbiohub.org/public/igem/BBa_K1486026/annotation2417052/range2417052/1
type: Range
persistent identity: range2417052
display id: range2417052
version: 1
direction: direction/forward
top level: BBa_K1486026
start: 2485
end: 2535
orientation: inline

8 a.a linker
type: SequenceAnnotation
persistent identity: annotation2417048
display id: annotation2417048
version: 1
top level: BBa_K1486026
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1486026/annotation2417048/range2417048/1

https://synbiohub.org/public/igem/BBa_K1486026/annotation2417048/range2417048/1
type: Range
persistent identity: range2417048
display id: range2417048
version: 1
direction: direction/forward
top level: BBa_K1486026
start: 51
end: 74
orientation: inline

BBa_K1015010
type: ComponentDefinition
persistent identity: BBa K1015010
display id: BBa_K1015010
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Ampicillin resistance gene
creator: Marina Ikeno
created: 2013-09-15T11:00:00Z
modified: 2016-01-28T01:51:04Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1321_
m user id: 4206
owner id: 13704
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Ampicillin resistance gene Ampicillin acts as an irreversible inhibitor of the enzyme transpeptidase, which is needed by bacteria to make their cell walls. It inhibits the third and final stage of bacterial cell wall synthesis in binary fission, which ultimately leads to cell lysis. This part includes a gene which has resistance to ampicillin. In our project, we use this part as a marker to select colonies of E.coli which succeeded in transformation.
mutable notes: selection maker
mutable provenance: plasmid(pI258) of Staphylococcus aureus
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1015010_sequence/1

BBa_K1930007
type: ComponentDefinition
persistent identity: BBa K1930007
display id: BBa_K1930007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Ciprofloxacin resistance cassette in integration plasmid (BBa_K823023)
creator: Barbora Waclawikova
created: 2016-10-15T11:00:00Z
modified: 2016-10-17T09:01:57Z
discontinued: true
dominant: false
experience: experience/Works
group u list: _2397_
m user id: 29748
owner id: 29748
owning group id: 9
status: status/Deleted
bookmark: false
mutable description: This part is a ciprofloxacin resistance cassette. It is containing a PAtpI constitutive promoter, ciprofloxacin resistance gene qnrS1 and double terminator. The promoter PAtpI ([http://parts.igem.org/Part:BBa_K1930005 ''BBa_K1930005'']) is a constitutive promoter which has its origin in Bacillus subtilis. It is responsible for the expression of atpA gene (ATP synthesis) during the first 30 minutes of the germination of B. subtilis. atpA gene is part of an operon (atpI-atpB-atpE-atpF-atpH-atpA-atpG-atpD-atpC), therefore the promoter region in front of the first protein coding gene (atpI) in this operon was chosen. Ciprofloxacin resistance gene qnrS1 is found naturally in E. coli, and other Gram-negative strains. qnr genes code for pentapeptide repeat proteins.??These proteins reduce susceptibility to quinolones by protecting the complex of DNA and DNA gyrase enzyme from the inhibitory effect of quinolones. Double terminator ([http://parts.igem.org/Part:BBa_B0015 ''BBa_B0015'']) is the most commonly used terminator. For more information see here: http://parts.igem.org/Part:BBa_B0015.
mutable notes: design
mutable provenance: Ciprofloxacin resistance cassette: Ordered as gBlock. IDT (Integrated DNA technologies). BBa_K823023: Created by iGEM Munich 2012. http://parts.igem.org/Part:BBa_K823023.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000155
role: partType/Plasmid
sequence annotation: QnrS1
sequence annotation: BBa_K1930005
sequence annotation: amyE
sequence annotation: BBa_B0015
sequence annotation: BBa_K823023
sequence annotation: bla
sequence annotation: cat
sequence annotation: 2TER
sequence annotation: P(AtpI)
sequence: https://synbiohub.org/public/igem/BBa_K1930007_sequence/1

QnrS1
type: SequenceAnnotation
persistent identity: annotation2514182
display id: annotation2514182
version: 1
top level: BBa_K1930007
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1930007/annotation2514182/range2514182/1

https://synbiohub.org/public/igem/BBa_K1930007/annotation2514182/range2514182/1
type: Range
persistent identity: range2514182
display id: range2514182
version: 1
direction: direction/forward
top level: BBa_K1930007
start: 351
end: 1007
orientation: inline

BBa_K1930005
type: SequenceAnnotation
persistent identity: annotation2514181
display id: annotation2514181
version: 1
top level: BBa_K1930007
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1930007/annotation2514181/range2514181/1

https://synbiohub.org/public/igem/BBa_K1930007/annotation2514181/range2514181/1
type: Range
persistent identity: range2514181
display id: range2514181
version: 1
direction: direction/forward
top level: BBa_K1930007
start: 1
end: 350
orientation: inline

amyE
type: SequenceAnnotation
persistent identity: annotation2514188
display id: annotation2514188
version: 1
top level: BBa_K1930007
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1930007/annotation2514188/range2514188/1

https://synbiohub.org/public/igem/BBa_K1930007/annotation2514188/range2514188/1
type: Range
persistent identity: range2514188
display id: range2514188
version: 1
direction: direction/forward
top level: BBa_K1930007
start: 6661
end: 7181
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2514198
display id: annotation2514198
version: 1
top level: BBa_K1930007
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1930007/annotation2514198/range2514198/1

https://synbiohub.org/public/igem/BBa_K1930007/annotation2514198/range2514198/1
type: Range
persistent identity: range2514198
display id: range2514198
version: 1
direction: direction/forward
top level: BBa_K1930007
start: 1008
end: 1136
orientation: inline

BBa_K823023
type: SequenceAnnotation
persistent identity: annotation2514184
display id: annotation2514184
version: 1
top level: BBa_K1930007
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1930007/annotation2514184/range2514184/1

https://synbiohub.org/public/igem/BBa_K1930007/annotation2514184/range2514184/1
type: Range
persistent identity: range2514184
display id: range2514184
version: 1
direction: direction/forward
top level: BBa_K1930007
start: 1137
end: 7198
orientation: inline

bla
type: SequenceAnnotation
persistent identity: annotation2514187
display id: annotation2514187
version: 1
top level: BBa_K1930007
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1930007/annotation2514187/range2514187/1

https://synbiohub.org/public/igem/BBa_K1930007/annotation2514187/range2514187/1
type: Range
persistent identity: range2514187
display id: range2514187
version: 1
direction: direction/forward
top level: BBa_K1930007
start: 5476
end: 6336
orientation: inline

cat
type: SequenceAnnotation
persistent identity: annotation2514185
display id: annotation2514185
version: 1
top level: BBa_K1930007
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1930007/annotation2514185/range2514185/1

https://synbiohub.org/public/igem/BBa_K1930007/annotation2514185/range2514185/1
type: Range
persistent identity: range2514185
display id: range2514185
version: 1
direction: direction/forward
top level: BBa_K1930007
start: 1473
end: 2123
orientation: inline

2TER
type: SequenceAnnotation
persistent identity: annotation2514183
display id: annotation2514183
version: 1
top level: BBa_K1930007
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1930007/annotation2514183/range2514183/1

https://synbiohub.org/public/igem/BBa_K1930007/annotation2514183/range2514183/1
type: Range
persistent identity: range2514183
display id: range2514183
version: 1
direction: direction/forward
top level: BBa_K1930007
start: 1008
end: 1136
orientation: inline

amyE
type: SequenceAnnotation
persistent identity: annotation2514186
display id: annotation2514186
version: 1
top level: BBa_K1930007
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1930007/annotation2514186/range2514186/1

https://synbiohub.org/public/igem/BBa_K1930007/annotation2514186/range2514186/1
type: Range
persistent identity: range2514186
display id: range2514186
version: 1
direction: direction/forward
top level: BBa_K1930007
start: 2494
end: 3522
orientation: inline

P(AtpI)
type: SequenceAnnotation
persistent identity: annotation2514180
display id: annotation2514180
version: 1
top level: BBa_K1930007
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1930007/annotation2514180/range2514180/1

https://synbiohub.org/public/igem/BBa_K1930007/annotation2514180/range2514180/1
type: Range
persistent identity: range2514180
display id: range2514180
version: 1
direction: direction/forward
top level: BBa_K1930007
start: 1
end: 350
orientation: inline

BBa_K678022
type: ComponentDefinition
persistent identity: BBa K678022
display id: BBa_K678022
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Neomycin cassette
creator: DTU-Denmark-2
created: 2011-09-03T11:00:00Z
modified: 2015-05-08T01:13:04Z
discontinued: false
dominant: false
experience: experience/None
group u list: _882_
m user id: 0
owner id: 8341
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Resistance cassette for selection of mammalian cells. The cassette contains a SV40 promoter, the neomycin resistance gene, and the SV40 poly A as a terminator.
mutable notes: Compatible with the Plug'n'Play assembly standard
mutable provenance: From plasmid pcDNA3.1 from Invitrogen
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Other
role: SO:0000110
sequence annotation: Neomycin cassette
sequence annotation: Linker 5
sequence: https://synbiohub.org/public/igem/BBa_K678022_sequence/1

Neomycin cassette
type: SequenceAnnotation
persistent identity: annotation2127359
display id: annotation2127359
version: 1
top level: BBa_K678022
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K678022/annotation2127359/range2127359/1

https://synbiohub.org/public/igem/BBa_K678022/annotation2127359/range2127359/1
type: Range
persistent identity: range2127359
display id: range2127359
version: 1
direction: direction/forward
top level: BBa_K678022
start: 9
end: 1512
orientation: inline

Linker 5
type: SequenceAnnotation
persistent identity: annotation2127358
display id: annotation2127358
version: 1
top level: BBa_K678022
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K678022/annotation2127358/range2127358/1

https://synbiohub.org/public/igem/BBa_K678022/annotation2127358/range2127358/1
type: Range
persistent identity: range2127358
display id: range2127358
version: 1
direction: direction/forward
top level: BBa_K678022
start: 1
end: 8
orientation: inline

BBa_K1486036
type: ComponentDefinition
persistent identity: BBa K1486036
display id: BBa_K1486036
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: rLucC + ADH1 terminator + CaUra3 cassette
creator: EPFL iGem team 2014
created: 2014-09-26T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: To edit
mutable notes: To edit
mutable provenance: To edit
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000324
role: partType/Tag
component: BBa_K1486037
component: BBa_K1486020
component: BBa_K1486031
component: BBa_K1486025
sequence annotation: BBa_K1486031
sequence annotation: BBa_K1486025
sequence annotation: BBa_K1486020
sequence annotation: BBa_K1486037
sequence: https://synbiohub.org/public/igem/BBa_K1486036_sequence/1

BBa_K1486037
type: Component
persistent identity: component2418337
display id: component2418337
version: 1
top level: BBa_K1486036
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486037/1

BBa_K1486020
type: Component
persistent identity: component2418338
display id: component2418338
version: 1
top level: BBa_K1486036
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486020/1

BBa_K1486031
type: Component
persistent identity: component2418343
display id: component2418343
version: 1
top level: BBa_K1486036
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486031/1

BBa_K1486025
type: Component
persistent identity: component2418339
display id: component2418339
version: 1
top level: BBa_K1486036
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486025/1

BBa_K1486031
type: SequenceAnnotation
persistent identity: annotation2418343
display id: annotation2418343
version: 1
top level: BBa_K1486036
location: https://synbiohub.org/public/igem/BBa_K1486036/annotation2418343/range2418343/1
component: BBa_K1486031

https://synbiohub.org/public/igem/BBa_K1486036/annotation2418343/range2418343/1
type: Range
persistent identity: range2418343
display id: range2418343
version: 1
direction: direction/reverse
top level: BBa_K1486036
start: 834
end: 2193
orientation: inline

BBa_K1486025
type: SequenceAnnotation
persistent identity: annotation2418339
display id: annotation2418339
version: 1
top level: BBa_K1486036
location: https://synbiohub.org/public/igem/BBa_K1486036/annotation2418339/range2418339/1
component: BBa_K1486025

https://synbiohub.org/public/igem/BBa_K1486036/annotation2418339/range2418339/1
type: Range
persistent identity: range2418339
display id: range2418339
version: 1
direction: direction/reverse
top level: BBa_K1486036
start: 646
end: 833
orientation: inline

BBa_K1486020
type: SequenceAnnotation
persistent identity: annotation2418338
display id: annotation2418338
version: 1
top level: BBa_K1486036
location: https://synbiohub.org/public/igem/BBa_K1486036/annotation2418338/range2418338/1
component: BBa_K1486020

https://synbiohub.org/public/igem/BBa_K1486036/annotation2418338/range2418338/1
type: Range
persistent identity: range2418338
display id: range2418338
version: 1
direction: direction/reverse
top level: BBa_K1486036
start: 40
end: 645
orientation: inline

BBa_K1486037
type: SequenceAnnotation
persistent identity: annotation2418337
display id: annotation2418337
version: 1
top level: BBa_K1486036
location: https://synbiohub.org/public/igem/BBa_K1486036/annotation2418337/range2418337/1
component: BBa_K1486037

https://synbiohub.org/public/igem/BBa_K1486036/annotation2418337/range2418337/1
type: Range
persistent identity: range2418337
display id: range2418337
version: 1
direction: direction/reverse
top level: BBa_K1486036
start: 1
end: 39
orientation: inline

CmR
type: ComponentDefinition
persistent identity: BBa J31005
display id: BBa_J31005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: chloramphenicol acetyltransferase (forwards, CmF) [cf. BBa_J31004]
creator: Erin Zwack, Sabriya Rosemond
created: 2006-07-11T11:00:00Z
modified: 2015-08-31T04:08:45Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _61_
m user id: 0
owner id: 918
owning group id: 61
sample status: In stock
status: status/Available
bookmark: true
mutable description: When a promoter and an RBS are in front of the gene, the cell will express Chloramphenicol resistance. Because it contains full biobrick ends, this part can be used to easily add chloramphenicol resistance to any part without changing plasmid vectors.
mutable notes: This part is cloned into pSB1A2.
mutable provenance: pSB1AC3
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: CmR gene
sequence: https://synbiohub.org/public/igem/BBa_J31005_sequence/1

CmR gene
type: SequenceAnnotation
persistent identity: annotation1884999
display id: annotation1884999
version: 1
top level: CmR
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J31005/annotation1884999/range1884999/1

https://synbiohub.org/public/igem/BBa_J31005/annotation1884999/range1884999/1
type: Range
persistent identity: range1884999
display id: range1884999
version: 1
direction: direction/forward
top level: CmR
start: 1
end: 660
orientation: inline

BBa_K1021003
type: ComponentDefinition
persistent identity: BBa K1021003
display id: BBa_K1021003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: bar (bialaphos resistance)
creator: Swati Sureka
created: 2013-08-29T11:00:00Z
modified: 2016-01-28T01:51:16Z
discontinued: false
dominant: false
group u list: _1328_
m user id: 4206
owner id: 13516
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: The bialaphos resistance (bar) gene confers resistance to bialaphos and phosphinothricin.
mutable notes: The plasmid sequence was unknown so extensive sequencing was carried out in order to extract it from its original vector, confirm BioBrick compatibility, and transfer it to pSB1C3.
mutable provenance: pBARGPE1 from the FGSC (http://www.fgsc.net/fgn/pall.html)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1021003_sequence/1

BBa_J133003
type: ComponentDefinition
persistent identity: BBa J133003
display id: BBa_J133003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: aadA gene with lacIQ promoter and RBS and oriT
creator: Norman Wang
created: 2011-07-11T11:00:00Z
modified: 2015-08-31T04:08:30Z
discontinued: false
dominant: false
group u list: _634_
m user id: 0
owner id: 2680
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: BBa_K125530 with an oriT postfixed, intermediate from the 2008 iGEM Hawaii team collection
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K125320
component: BBa_K125530
sequence annotation: BBa_K125530
sequence annotation: BBa_K125320
sequence: https://synbiohub.org/public/igem/BBa_J133003_sequence/1

BBa_K125320
type: Component
persistent identity: component2123319
display id: component2123319
version: 1
top level: BBa_J133003
access: public
definition: https://synbiohub.org/public/igem/BBa_K125320/1

BBa_K125530
type: Component
persistent identity: component2123318
display id: component2123318
version: 1
top level: BBa_J133003
access: public
definition: https://synbiohub.org/public/igem/BBa_K125530/1

BBa_K125530
type: SequenceAnnotation
persistent identity: annotation2123318
display id: annotation2123318
version: 1
top level: BBa_J133003
location: https://synbiohub.org/public/igem/BBa_J133003/annotation2123318/range2123318/1
component: BBa_K125530

https://synbiohub.org/public/igem/BBa_J133003/annotation2123318/range2123318/1
type: Range
persistent identity: range2123318
display id: range2123318
version: 1
direction: direction/reverse
top level: BBa_J133003
start: 1
end: 855
orientation: inline

BBa_K125320
type: SequenceAnnotation
persistent identity: annotation2123319
display id: annotation2123319
version: 1
top level: BBa_J133003
location: https://synbiohub.org/public/igem/BBa_J133003/annotation2123319/range2123319/1
component: BBa_K125320

https://synbiohub.org/public/igem/BBa_J133003/annotation2123319/range2123319/1
type: Range
persistent identity: range2123319
display id: range2123319
version: 1
direction: direction/reverse
top level: BBa_J133003
start: 864
end: 962
orientation: inline

kanR
type: ComponentDefinition
persistent identity: BBa K389005
display id: BBa_K389005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Kanamycin resistance
creator: Jonas Aretz
created: 2010-08-09T11:00:00Z
modified: 2015-05-08T01:12:19Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _517_
m user id: 0
owner id: 6670
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This is a kanamycin resistance part without promotor. It is made from the BioBrick BBa_P1003 and is used for screenings.
mutable notes: we removed the promotor from the kanamycin resistance gene in BioBrick BBa_P1003
mutable provenance: BioBrick BBa_P1003
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: kanamycin resistance
sequence annotation: Stop codon
sequence: https://synbiohub.org/public/igem/BBa_K389005_sequence/1

kanamycin resistance
type: SequenceAnnotation
persistent identity: annotation2077058
display id: annotation2077058
version: 1
top level: kanR
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K389005/annotation2077058/range2077058/1

https://synbiohub.org/public/igem/BBa_K389005/annotation2077058/range2077058/1
type: Range
persistent identity: range2077058
display id: range2077058
version: 1
direction: direction/forward
top level: kanR
start: 1
end: 816
orientation: inline

Stop codon
type: SequenceAnnotation
persistent identity: annotation2078365
display id: annotation2078365
version: 1
top level: kanR
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K389005/annotation2078365/range2078365/1

https://synbiohub.org/public/igem/BBa_K389005/annotation2078365/range2078365/1
type: Range
persistent identity: range2078365
display id: range2078365
version: 1
direction: direction/forward
top level: kanR
start: 817
end: 819
orientation: inline

KanR Backw
type: ComponentDefinition
persistent identity: BBa J31002
display id: BBa_J31002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: kanamycin resistance backwards (KanB) [cf. BBa_J23012 & BBa_J31003]
creator: Erin Zwack, Sabriya Rosemond
created: 2006-07-11T11:00:00Z
modified: 2015-08-31T04:08:45Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _61_
m user id: 0
owner id: 918
owning group id: 61
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Codes the reverse of the Kanamycin Resistance gene. When the region is flipped, Kanamycin resistance will be expressed as long as a promoter and RBS are in front of the region.
mutable notes: None
mutable provenance: pSB1AK3
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Reverse of KanR gene
sequence: https://synbiohub.org/public/igem/BBa_J31002_sequence/1

Reverse of KanR gene
type: SequenceAnnotation
persistent identity: annotation1884995
display id: annotation1884995
version: 1
top level: KanR Backw
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J31002/annotation1884995/range1884995/1

https://synbiohub.org/public/igem/BBa_J31002/annotation1884995/range1884995/1
type: Range
persistent identity: range1884995
display id: range1884995
version: 1
direction: direction/reverse
top level: KanR Backw
start: 1
end: 816
orientation: inline

tetA(C)f
type: ComponentDefinition
persistent identity: BBa J31007
display id: BBa_J31007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: tetracycline resistance protein TetA(C) (forward), [cf. BBa_J31006]
creator: Sabriya Rosemond, Erin Zwack
created: 2006-07-11T11:00:00Z
modified: 2015-08-31T04:08:45Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _61_
m user id: 0
owner id: 919
owning group id: 61
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This part is the coding region of the TetR gene. It requires a promoter and RBS for the cell to be able to express tetracyline resistance.
mutable notes: It was cloned into PsB1A2 plamsid.
mutable provenance: PsB1AT3
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Rev primer J31007
sequence annotation: Fwd primer J31007
sequence annotation: stop
sequence annotation: tetA(C) forward
sequence: https://synbiohub.org/public/igem/BBa_J31007_sequence/1

Rev primer J31007
type: SequenceAnnotation
persistent identity: annotation1910572
display id: annotation1910572
version: 1
top level: tetA(C)f
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_J31007/annotation1910572/range1910572/1

https://synbiohub.org/public/igem/BBa_J31007/annotation1910572/range1910572/1
type: Range
persistent identity: range1910572
display id: range1910572
version: 1
direction: direction/reverse
top level: tetA(C)f
start: 1172
end: 1191
orientation: inline

Fwd primer J31007
type: SequenceAnnotation
persistent identity: annotation1910571
display id: annotation1910571
version: 1
top level: tetA(C)f
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_J31007/annotation1910571/range1910571/1

https://synbiohub.org/public/igem/BBa_J31007/annotation1910571/range1910571/1
type: Range
persistent identity: range1910571
display id: range1910571
version: 1
direction: direction/forward
top level: tetA(C)f
start: 1
end: 24
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1910573
display id: annotation1910573
version: 1
top level: tetA(C)f
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_J31007/annotation1910573/range1910573/1

https://synbiohub.org/public/igem/BBa_J31007/annotation1910573/range1910573/1
type: Range
persistent identity: range1910573
display id: range1910573
version: 1
direction: direction/forward
top level: tetA(C)f
start: 1189
end: 1191
orientation: inline

tetA(C) forward
type: SequenceAnnotation
persistent identity: annotation1885000
display id: annotation1885000
version: 1
top level: tetA(C)f
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J31007/annotation1885000/range1885000/1

https://synbiohub.org/public/igem/BBa_J31007/annotation1885000/range1885000/1
type: Range
persistent identity: range1885000
display id: range1885000
version: 1
direction: direction/forward
top level: tetA(C)f
start: 1
end: 1191
orientation: inline

KanR
type: ComponentDefinition
persistent identity: BBa J31003
display id: BBa_J31003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: kanamycin resistance forward (KanF) [cf. BBa_J23012 & BBa_J31002]
creator: Erin Zwack, Sabriya Rosemond
created: 2006-07-11T11:00:00Z
modified: 2015-08-31T04:08:45Z
discontinued: false
dominant: false
experience: experience/None
group u list: _61_
m user id: 0
owner id: 918
owning group id: 61
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: If there is a promoter and an RBS before this region, the cell will express Kanamycin resistance. As it has full biobricks, you can easily add KanR to an existing part without changing the plasmid vector,
mutable notes: This part is cloned in plasmid pSB1A2.
mutable provenance: pSB1AK3
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: KanR Gene
sequence: https://synbiohub.org/public/igem/BBa_J31003_sequence/1

KanR Gene
type: SequenceAnnotation
persistent identity: annotation1884996
display id: annotation1884996
version: 1
top level: KanR
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J31003/annotation1884996/range1884996/1

https://synbiohub.org/public/igem/BBa_J31003/annotation1884996/range1884996/1
type: Range
persistent identity: range1884996
display id: range1884996
version: 1
direction: direction/forward
top level: KanR
start: 1
end: 816
orientation: inline

CmR Backwa
type: ComponentDefinition
persistent identity: BBa J31004
display id: BBa_J31004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: chloramphenicol acetyltransferase (backwards, CmB) [cf. BBa_J31005]
creator: Erin Zwack, Sabriya Rosemond
created: 2006-07-11T11:00:00Z
modified: 2015-08-31T04:08:45Z
discontinued: false
dominant: false
experience: experience/None
group u list: _61_
m user id: 0
owner id: 918
owning group id: 61
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Codes the reverse of the CmR gene. If the region has a promoter and an RBS in front and is flipped, the cell will express Chloramphenicol resistance.
mutable notes: This part was cloned pSB1A2.
mutable provenance: pSB1AC3
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Reverse of CmR gene
sequence: https://synbiohub.org/public/igem/BBa_J31004_sequence/1

Reverse of CmR gene
type: SequenceAnnotation
persistent identity: annotation1884997
display id: annotation1884997
version: 1
top level: CmR Backwa
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J31004/annotation1884997/range1884997/1

https://synbiohub.org/public/igem/BBa_J31004/annotation1884997/range1884997/1
type: Range
persistent identity: range1884997
display id: range1884997
version: 1
direction: direction/forward
top level: CmR Backwa
start: 1
end: 660
orientation: inline

BBa_K156011
type: ComponentDefinition
persistent identity: BBa K156011
display id: BBa_K156011
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: aadA (streptomycin 3'-adenyltransferase)
creator: George McArthur IV
created: 2008-10-28T12:00:00Z
modified: 2015-05-08T01:10:54Z
discontinued: false
dominant: false
experience: experience/None
group u list: _246_
m user id: 0
owner id: 1475
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part codes for an aminoglycoside resistance protein that endows ''E. coli'' with resistance to the antibiotic streptomycin. This part can be used as a selection marker.
mutable notes: This part was codon-optimized for expression in E. coli. The following restriction enzyme sites were also removed: EcoRI, NotI, XbaI, SpeI, PstI, NheI, AvrII, ApoI, MfeI, NsiI, SbfI.
mutable provenance: aadA was isolated from a plasmid in ''E. coli''. The original sequence was obtained from GenBank (VERSION NC_002525.1, GI:10955438).
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K156011_sequence/1

BBa_K1323011
type: ComponentDefinition
persistent identity: BBa K1323011
display id: BBa_K1323011
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Erythromycin resistance coding sequence
creator: Julia Manalil
created: 2014-10-03T11:00:00Z
modified: 2016-01-28T01:42:45Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1698_
m user id: 4206
owner id: 18368
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: TBA
mutable notes: TBA
mutable provenance: TBA
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: ermM CDS (Erythromycin resistance)
sequence: https://synbiohub.org/public/igem/BBa_K1323011_sequence/1

ermM CDS (Erythromycin resistance)
type: SequenceAnnotation
persistent identity: annotation2428789
display id: annotation2428789
version: 1
top level: BBa_K1323011
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1323011/annotation2428789/range2428789/1

https://synbiohub.org/public/igem/BBa_K1323011/annotation2428789/range2428789/1
type: Range
persistent identity: range2428789
display id: range2428789
version: 1
direction: direction/forward
top level: BBa_K1323011
start: 1
end: 735
orientation: inline

BBa_K914018
type: ComponentDefinition
persistent identity: BBa K914018
display id: BBa_K914018
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: P1003** Kan resistant gene with 2 Amber Codon
creator: Jean Cury
created: 2012-10-24T11:00:00Z
modified: 2015-05-08T01:13:46Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1179_
m user id: 0
owner id: 13470
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: The amber mutations avoid the expression of the P1003 gene even at usual concentration that prevent the expression of kanamycin resistance, although one mutation (BBa_K914009) permits some leakage in translation for this gene. This mutation is rescued with the tRNA amber suppressor supD (BBa_K914000). The idea here is to prevent the kanamycin gene resistance to be expressed in case horizontal gene transfer.
mutable notes: I have need to design the primers that will introduce a mutation from a serine that was closer to the TAG codon (either 1, 2 or 3 mutations, here it was 1 and 2 mutations for both mutations), and it is necessary to have the amber codon in the middle of the coding sequence, not in the beginning, to be not interpreted as a alternative start codon for instance.
mutable provenance: This part is made from the BBa_K914009 part from which I add a second mutation with the QuickChange Site-directed mutagenesis kit, Agilent.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
sequence annotation: Ser133->Amber codon
sequence annotation: Ser203->Amber mutation
sequence annotation: BBa_P1003
sequence annotation: kanamycin resistance
sequence annotation: BBa_K914009
sequence: https://synbiohub.org/public/igem/BBa_K914018_sequence/1

Ser133->Amber codon
type: SequenceAnnotation
persistent identity: annotation2212596
display id: annotation2212596
version: 1
top level: BBa_K914018
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_K914018/annotation2212596/range2212596/1

https://synbiohub.org/public/igem/BBa_K914018/annotation2212596/range2212596/1
type: Range
persistent identity: range2212596
display id: range2212596
version: 1
direction: direction/forward
top level: BBa_K914018
start: 546
end: 546
orientation: inline

Ser203->Amber mutation
type: SequenceAnnotation
persistent identity: annotation2212599
display id: annotation2212599
version: 1
top level: BBa_K914018
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K914018/annotation2212599/range2212599/1

https://synbiohub.org/public/igem/BBa_K914018/annotation2212599/range2212599/1
type: Range
persistent identity: range2212599
display id: range2212599
version: 1
direction: direction/forward
top level: BBa_K914018
start: 609
end: 610
orientation: inline

BBa_P1003
type: SequenceAnnotation
persistent identity: annotation2212594
display id: annotation2212594
version: 1
top level: BBa_K914018
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K914018/annotation2212594/range2212594/1

https://synbiohub.org/public/igem/BBa_K914018/annotation2212594/range2212594/1
type: Range
persistent identity: range2212594
display id: range2212594
version: 1
direction: direction/forward
top level: BBa_K914018
start: 1
end: 967
orientation: inline

kanamycin resistance
type: SequenceAnnotation
persistent identity: annotation2212595
display id: annotation2212595
version: 1
top level: BBa_K914018
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K914018/annotation2212595/range2212595/1

https://synbiohub.org/public/igem/BBa_K914018/annotation2212595/range2212595/1
type: Range
persistent identity: range2212595
display id: range2212595
version: 1
direction: direction/forward
top level: BBa_K914018
start: 149
end: 967
orientation: inline

BBa_K914009
type: SequenceAnnotation
persistent identity: annotation2212597
display id: annotation2212597
version: 1
top level: BBa_K914018
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K914018/annotation2212597/range2212597/1

https://synbiohub.org/public/igem/BBa_K914018/annotation2212597/range2212597/1
type: Range
persistent identity: range2212597
display id: range2212597
version: 1
direction: direction/reverse
top level: BBa_K914018
start: 1
end: 967
orientation: inline

BBa_K1930003
type: ComponentDefinition
persistent identity: BBa K1930003
display id: BBa_K1930003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Encrypted message sequence in integration plasmid (BBa_K823023)
creator: Barbora Waclawikova
created: 2016-10-15T11:00:00Z
modified: 2016-10-17T09:01:43Z
discontinued: true
dominant: false
experience: experience/Works
group u list: _2397_
m user id: 29748
owner id: 29748
owning group id: 9
status: status/Deleted
bookmark: false
mutable description: The iGEM team Groningen 2016 worked on bioencryption in order to safely store data in DNA. In our project we were mainly working with two sequences of DNA which were the encrypted message sequence and the encryption key sequence. The message sequence was created by our software. It is therefore artificial DNA. We ordered the encrypted message sequence as gBlock from IDT (Integrated DNA technologies). This part was created in the interest of having a genomic integration plasmid with our message sequence. We used an available BioBrick BBa_K823023 from igem Munich 2012. It is an integration plasmid for Bacillus subtilis, which can be used for cloning in E. coli as well. An RFP is inserted in BBa_K823023 for more efficient screening after transformation.
mutable notes: The iGEM team Groningen 2016 worked on bioencryption in order to safely store data in DNA. In our project we were mainly working with two sequences of DNA which were the encrypted message sequence and the encryption key sequence. The message sequence was created by our software. It is therefore artificial DNA. We ordered the encrypted message sequence as gBlock from IDT (Integrated DNA technologies). This part was created in the interest of having a genomic integration plasmid with our message sequence. We used an available BioBrick BBa_K823023 from igem Munich 2012. It is an integration plasmid for Bacillus subtilis, which can be used for cloning in E. coli as well. An RFP is inserted in BBa_K823023 for more efficient screening after transformation. For more information see plasmid construction under our lab journal: http://2016.igem.org/Team:Groningen/Experiments
mutable provenance: Message sequence: Created by our CryptoGErM software. http://2016.igem.org/Team:Groningen/Encoding BBa_K823023: Created by iGEM Munich 2012. http://parts.igem.org/Part:BBa_K823023
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000155
role: partType/Plasmid
sequence annotation: message sequence
sequence annotation: amyE
sequence annotation: cat
sequence annotation: BBa_K1930001
sequence annotation: BBa_K823023
sequence annotation: bla
sequence: https://synbiohub.org/public/igem/BBa_K1930003_sequence/1

message sequence
type: SequenceAnnotation
persistent identity: annotation2513445
display id: annotation2513445
version: 1
top level: BBa_K1930003
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1930003/annotation2513445/range2513445/1

https://synbiohub.org/public/igem/BBa_K1930003/annotation2513445/range2513445/1
type: Range
persistent identity: range2513445
display id: range2513445
version: 1
direction: direction/forward
top level: BBa_K1930003
start: 1
end: 556
orientation: inline

amyE
type: SequenceAnnotation
persistent identity: annotation2513447
display id: annotation2513447
version: 1
top level: BBa_K1930003
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1930003/annotation2513447/range2513447/1

https://synbiohub.org/public/igem/BBa_K1930003/annotation2513447/range2513447/1
type: Range
persistent identity: range2513447
display id: range2513447
version: 1
direction: direction/forward
top level: BBa_K1930003
start: 1914
end: 2942
orientation: inline

cat
type: SequenceAnnotation
persistent identity: annotation2513446
display id: annotation2513446
version: 1
top level: BBa_K1930003
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1930003/annotation2513446/range2513446/1

https://synbiohub.org/public/igem/BBa_K1930003/annotation2513446/range2513446/1
type: Range
persistent identity: range2513446
display id: range2513446
version: 1
direction: direction/forward
top level: BBa_K1930003
start: 893
end: 1543
orientation: inline

BBa_K1930001
type: SequenceAnnotation
persistent identity: annotation2514197
display id: annotation2514197
version: 1
top level: BBa_K1930003
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1930003/annotation2514197/range2514197/1

https://synbiohub.org/public/igem/BBa_K1930003/annotation2514197/range2514197/1
type: Range
persistent identity: range2514197
display id: range2514197
version: 1
direction: direction/forward
top level: BBa_K1930003
start: 1
end: 556
orientation: inline

BBa_K823023
type: SequenceAnnotation
persistent identity: annotation2513444
display id: annotation2513444
version: 1
top level: BBa_K1930003
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1930003/annotation2513444/range2513444/1

https://synbiohub.org/public/igem/BBa_K1930003/annotation2513444/range2513444/1
type: Range
persistent identity: range2513444
display id: range2513444
version: 1
direction: direction/forward
top level: BBa_K1930003
start: 557
end: 6618
orientation: inline

bla
type: SequenceAnnotation
persistent identity: annotation2513448
display id: annotation2513448
version: 1
top level: BBa_K1930003
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1930003/annotation2513448/range2513448/1

https://synbiohub.org/public/igem/BBa_K1930003/annotation2513448/range2513448/1
type: Range
persistent identity: range2513448
display id: range2513448
version: 1
direction: direction/forward
top level: BBa_K1930003
start: 4896
end: 5756
orientation: inline

amyE
type: SequenceAnnotation
persistent identity: annotation2513449
display id: annotation2513449
version: 1
top level: BBa_K1930003
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1930003/annotation2513449/range2513449/1

https://synbiohub.org/public/igem/BBa_K1930003/annotation2513449/range2513449/1
type: Range
persistent identity: range2513449
display id: range2513449
version: 1
direction: direction/forward
top level: BBa_K1930003
start: 6081
end: 6601
orientation: inline

BBa_K1486029
type: ComponentDefinition
persistent identity: BBa K1486029
display id: BBa_K1486029
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Yeast Optimized sfGFP-N + ADH1 terminator + Kanamycin resistance
creator: EPFL iGem team 2014
created: 2014-09-26T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: To edit
mutable notes: To edit
mutable provenance: To edit
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K1486025
component: BBa_K1486028
component: BBa_K1486037
component: BBa_K1486024
sequence annotation: BBa_K1486028
sequence annotation: BBa_K1486024
sequence annotation: BBa_K1486037
sequence annotation: BBa_K1486025
sequence: https://synbiohub.org/public/igem/BBa_K1486029_sequence/1

BBa_K1486025
type: Component
persistent identity: component2417700
display id: component2417700
version: 1
top level: BBa_K1486029
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486025/1

BBa_K1486028
type: Component
persistent identity: component2417699
display id: component2417699
version: 1
top level: BBa_K1486029
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486028/1

BBa_K1486037
type: Component
persistent identity: component2417697
display id: component2417697
version: 1
top level: BBa_K1486029
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486037/1

BBa_K1486024
type: Component
persistent identity: component2417704
display id: component2417704
version: 1
top level: BBa_K1486029
access: public
definition: BBa_K1486024

BBa_K1486028
type: SequenceAnnotation
persistent identity: annotation2417699
display id: annotation2417699
version: 1
top level: BBa_K1486029
location: https://synbiohub.org/public/igem/BBa_K1486029/annotation2417699/range2417699/1
component: BBa_K1486028

https://synbiohub.org/public/igem/BBa_K1486029/annotation2417699/range2417699/1
type: Range
persistent identity: range2417699
display id: range2417699
version: 1
direction: direction/reverse
top level: BBa_K1486029
start: 40
end: 681
orientation: inline

BBa_K1486024
type: SequenceAnnotation
persistent identity: annotation2417704
display id: annotation2417704
version: 1
top level: BBa_K1486029
location: https://synbiohub.org/public/igem/BBa_K1486029/annotation2417704/range2417704/1
component: BBa_K1486024

https://synbiohub.org/public/igem/BBa_K1486029/annotation2417704/range2417704/1
type: Range
persistent identity: range2417704
display id: range2417704
version: 1
direction: direction/reverse
top level: BBa_K1486029
start: 870
end: 2226
orientation: inline

BBa_K1486037
type: SequenceAnnotation
persistent identity: annotation2417697
display id: annotation2417697
version: 1
top level: BBa_K1486029
location: https://synbiohub.org/public/igem/BBa_K1486029/annotation2417697/range2417697/1
component: BBa_K1486037

https://synbiohub.org/public/igem/BBa_K1486029/annotation2417697/range2417697/1
type: Range
persistent identity: range2417697
display id: range2417697
version: 1
direction: direction/reverse
top level: BBa_K1486029
start: 1
end: 39
orientation: inline

BBa_K1486025
type: SequenceAnnotation
persistent identity: annotation2417700
display id: annotation2417700
version: 1
top level: BBa_K1486029
location: https://synbiohub.org/public/igem/BBa_K1486029/annotation2417700/range2417700/1
component: BBa_K1486025

https://synbiohub.org/public/igem/BBa_K1486029/annotation2417700/range2417700/1
type: Range
persistent identity: range2417700
display id: range2417700
version: 1
direction: direction/reverse
top level: BBa_K1486029
start: 682
end: 869
orientation: inline

BBa_K319041
type: ComponentDefinition
persistent identity: BBa K319041
display id: BBa_K319041
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: NatMX cassette
creator: Afnan Azizi
created: 2010-10-25T11:00:00Z
modified: 2015-05-08T01:11:59Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _437_
m user id: 0
owner id: 7363
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This is the cassette (promoter+coding region+terminator) that imparts resistance to nourseothricin in yeast.
mutable notes: N/A
mutable provenance: n
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: TEF1 promoter
sequence annotation: NatMX ORF
sequence annotation: TEF1 terminator
sequence: https://synbiohub.org/public/igem/BBa_K319041_sequence/1

TEF1 promoter
type: SequenceAnnotation
persistent identity: annotation2109281
display id: annotation2109281
version: 1
top level: BBa_K319041
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K319041/annotation2109281/range2109281/1

https://synbiohub.org/public/igem/BBa_K319041/annotation2109281/range2109281/1
type: Range
persistent identity: range2109281
display id: range2109281
version: 1
direction: direction/forward
top level: BBa_K319041
start: 1
end: 379
orientation: inline

NatMX ORF
type: SequenceAnnotation
persistent identity: annotation2109282
display id: annotation2109282
version: 1
top level: BBa_K319041
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K319041/annotation2109282/range2109282/1

https://synbiohub.org/public/igem/BBa_K319041/annotation2109282/range2109282/1
type: Range
persistent identity: range2109282
display id: range2109282
version: 1
direction: direction/forward
top level: BBa_K319041
start: 380
end: 955
orientation: inline

TEF1 terminator
type: SequenceAnnotation
persistent identity: annotation2109283
display id: annotation2109283
version: 1
top level: BBa_K319041
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K319041/annotation2109283/range2109283/1

https://synbiohub.org/public/igem/BBa_K319041/annotation2109283/range2109283/1
type: Range
persistent identity: range2109283
display id: range2109283
version: 1
direction: direction/forward
top level: BBa_K319041
start: 956
end: 1190
orientation: inline

BBa_K1486030
type: ComponentDefinition
persistent identity: BBa K1486030
display id: BBa_K1486030
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: rLucN + ADH1 terminator + Kanamycin resistance
creator: EPFL iGem team 2014
created: 2014-09-26T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: To edit
mutable notes: To edit
mutable provenance: To edit
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K1486019
component: BBa_K1486024
component: BBa_K1486025
component: BBa_K1486037
sequence annotation: BBa_K1486037
sequence annotation: BBa_K1486019
sequence annotation: BBa_K1486025
sequence annotation: BBa_K1486024
sequence: https://synbiohub.org/public/igem/BBa_K1486030_sequence/1

BBa_K1486019
type: Component
persistent identity: component2417717
display id: component2417717
version: 1
top level: BBa_K1486030
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486019/1

BBa_K1486024
type: Component
persistent identity: component2417722
display id: component2417722
version: 1
top level: BBa_K1486030
access: public
definition: BBa_K1486024

BBa_K1486025
type: Component
persistent identity: component2417718
display id: component2417718
version: 1
top level: BBa_K1486030
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486025/1

BBa_K1486037
type: Component
persistent identity: component2417716
display id: component2417716
version: 1
top level: BBa_K1486030
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486037/1

BBa_K1486037
type: SequenceAnnotation
persistent identity: annotation2417716
display id: annotation2417716
version: 1
top level: BBa_K1486030
location: https://synbiohub.org/public/igem/BBa_K1486030/annotation2417716/range2417716/1
component: BBa_K1486037

https://synbiohub.org/public/igem/BBa_K1486030/annotation2417716/range2417716/1
type: Range
persistent identity: range2417716
display id: range2417716
version: 1
direction: direction/reverse
top level: BBa_K1486030
start: 1
end: 39
orientation: inline

BBa_K1486019
type: SequenceAnnotation
persistent identity: annotation2417717
display id: annotation2417717
version: 1
top level: BBa_K1486030
location: https://synbiohub.org/public/igem/BBa_K1486030/annotation2417717/range2417717/1
component: BBa_K1486019

https://synbiohub.org/public/igem/BBa_K1486030/annotation2417717/range2417717/1
type: Range
persistent identity: range2417717
display id: range2417717
version: 1
direction: direction/reverse
top level: BBa_K1486030
start: 40
end: 369
orientation: inline

BBa_K1486025
type: SequenceAnnotation
persistent identity: annotation2417718
display id: annotation2417718
version: 1
top level: BBa_K1486030
location: https://synbiohub.org/public/igem/BBa_K1486030/annotation2417718/range2417718/1
component: BBa_K1486025

https://synbiohub.org/public/igem/BBa_K1486030/annotation2417718/range2417718/1
type: Range
persistent identity: range2417718
display id: range2417718
version: 1
direction: direction/reverse
top level: BBa_K1486030
start: 370
end: 557
orientation: inline

BBa_K1486024
type: SequenceAnnotation
persistent identity: annotation2417722
display id: annotation2417722
version: 1
top level: BBa_K1486030
location: https://synbiohub.org/public/igem/BBa_K1486030/annotation2417722/range2417722/1
component: BBa_K1486024

https://synbiohub.org/public/igem/BBa_K1486030/annotation2417722/range2417722/1
type: Range
persistent identity: range2417722
display id: range2417722
version: 1
direction: direction/reverse
top level: BBa_K1486030
start: 558
end: 1914
orientation: inline

BBa_K914009
type: ComponentDefinition
persistent identity: BBa K914009
display id: BBa_K914009
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: P1003* Ser133->Amber Codon
creator: Jean Cury
created: 2012-09-20T11:00:00Z
modified: 2015-05-08T01:13:46Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1179_
m user id: 0
owner id: 13470
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: The single mutation avoid the expression of the P1003 gene, that prevent the expression of a kanamycin resistance. This mutation is rescue with the tRNA amber suppressor supD (BBa_K914000), or any genotype relative to sup44 . The idea here is to prevent the kanamycin gene resistance in case horizontal gene transfer. Actually it would be better with another mutation.
mutable notes: I made the single point mutation with a quick change directed mutagenesis kit.
mutable provenance: this part comes from BBa_P1003
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
sequence annotation: kanamycin resistance
sequence annotation: Ser133->Amber codon
sequence annotation: BBa_P1003
sequence: https://synbiohub.org/public/igem/BBa_K914009_sequence/1

kanamycin resistance
type: SequenceAnnotation
persistent identity: annotation2212601
display id: annotation2212601
version: 1
top level: BBa_K914009
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K914009/annotation2212601/range2212601/1

https://synbiohub.org/public/igem/BBa_K914009/annotation2212601/range2212601/1
type: Range
persistent identity: range2212601
display id: range2212601
version: 1
direction: direction/forward
top level: BBa_K914009
start: 149
end: 967
orientation: inline

Ser133->Amber codon
type: SequenceAnnotation
persistent identity: annotation2212602
display id: annotation2212602
version: 1
top level: BBa_K914009
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_K914009/annotation2212602/range2212602/1

https://synbiohub.org/public/igem/BBa_K914009/annotation2212602/range2212602/1
type: Range
persistent identity: range2212602
display id: range2212602
version: 1
direction: direction/forward
top level: BBa_K914009
start: 546
end: 546
orientation: inline

BBa_P1003
type: SequenceAnnotation
persistent identity: annotation2212600
display id: annotation2212600
version: 1
top level: BBa_K914009
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K914009/annotation2212600/range2212600/1

https://synbiohub.org/public/igem/BBa_K914009/annotation2212600/range2212600/1
type: Range
persistent identity: range2212600
display id: range2212600
version: 1
direction: direction/forward
top level: BBa_K914009
start: 1
end: 967
orientation: inline

BBa_T9000
type: ComponentDefinition
persistent identity: BBa T9000
display id: BBa_T9000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: aacC2
creator: Abigail Sison
created: 2016-08-21T11:00:00Z
modified: 2016-08-22T10:11:14Z
discontinued: false
dominant: false
group u list: _1_
m user id: 33565
owner id: 33565
owning group id: 9
status: status/Planning
bookmark: false
mutable description: E.coli R-plasmid aacC2 gene for aminoglycoside-(3)-N-acetyltransferase activity AKA Gentamicin resistance
mutable notes: Changed TAG stop codon to TAA
mutable provenance: Escherichia coli R-Plasmid. Sequence from Genbank: http://www.ncbi.nlm.nih.gov/nuccore/X54723
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: ATG Start Codon
sequence annotation: aacC2 coding region
sequence annotation: TAA Stop Codon
sequence: https://synbiohub.org/public/igem/BBa_T9000_sequence/1

ATG Start Codon
type: SequenceAnnotation
persistent identity: annotation2481500
display id: annotation2481500
version: 1
top level: BBa_T9000
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_T9000/annotation2481500/range2481500/1

https://synbiohub.org/public/igem/BBa_T9000/annotation2481500/range2481500/1
type: Range
persistent identity: range2481500
display id: range2481500
version: 1
direction: direction/forward
top level: BBa_T9000
start: 1
end: 3
orientation: inline

aacC2 coding region
type: SequenceAnnotation
persistent identity: annotation2481501
display id: annotation2481501
version: 1
top level: BBa_T9000
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_T9000/annotation2481501/range2481501/1

https://synbiohub.org/public/igem/BBa_T9000/annotation2481501/range2481501/1
type: Range
persistent identity: range2481501
display id: range2481501
version: 1
direction: direction/forward
top level: BBa_T9000
start: 1
end: 861
orientation: inline

TAA Stop Codon
type: SequenceAnnotation
persistent identity: annotation2481502
display id: annotation2481502
version: 1
top level: BBa_T9000
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_T9000/annotation2481502/range2481502/1

https://synbiohub.org/public/igem/BBa_T9000/annotation2481502/range2481502/1
type: Range
persistent identity: range2481502
display id: range2481502
version: 1
direction: direction/forward
top level: BBa_T9000
start: 859
end: 861
orientation: inline

BBa_K1486033
type: ComponentDefinition
persistent identity: BBa K1486033
display id: BBa_K1486033
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: R.reniformis luciferase + ADH1 terminator + CaUra3
creator: EPFL iGem team 2014
created: 2014-09-26T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: To edit
mutable notes: To edit
mutable provenance: To edit
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000324
role: partType/Tag
component: BBa_K1486020
component: BBa_K1486025
component: BBa_K1486031
component: BBa_K1486037
component: BBa_K1486019
sequence annotation: BBa_K1486037
sequence annotation: BBa_K1486031
sequence annotation: BBa_K1486025
sequence annotation: BBa_K1486020
sequence annotation: BBa_K1486019
sequence: https://synbiohub.org/public/igem/BBa_K1486033_sequence/1

BBa_K1486020
type: Component
persistent identity: component2418784
display id: component2418784
version: 1
top level: BBa_K1486033
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486020/1

BBa_K1486025
type: Component
persistent identity: component2418786
display id: component2418786
version: 1
top level: BBa_K1486033
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486025/1

BBa_K1486031
type: Component
persistent identity: component2418790
display id: component2418790
version: 1
top level: BBa_K1486033
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486031/1

BBa_K1486037
type: Component
persistent identity: component2418782
display id: component2418782
version: 1
top level: BBa_K1486033
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486037/1

BBa_K1486019
type: Component
persistent identity: component2418783
display id: component2418783
version: 1
top level: BBa_K1486033
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486019/1

BBa_K1486037
type: SequenceAnnotation
persistent identity: annotation2418782
display id: annotation2418782
version: 1
top level: BBa_K1486033
location: https://synbiohub.org/public/igem/BBa_K1486033/annotation2418782/range2418782/1
component: BBa_K1486037

https://synbiohub.org/public/igem/BBa_K1486033/annotation2418782/range2418782/1
type: Range
persistent identity: range2418782
display id: range2418782
version: 1
direction: direction/reverse
top level: BBa_K1486033
start: 1
end: 39
orientation: inline

BBa_K1486031
type: SequenceAnnotation
persistent identity: annotation2418790
display id: annotation2418790
version: 1
top level: BBa_K1486033
location: https://synbiohub.org/public/igem/BBa_K1486033/annotation2418790/range2418790/1
component: BBa_K1486031

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type: Range
persistent identity: range2418790
display id: range2418790
version: 1
direction: direction/reverse
top level: BBa_K1486033
start: 1164
end: 2523
orientation: inline

BBa_K1486025
type: SequenceAnnotation
persistent identity: annotation2418786
display id: annotation2418786
version: 1
top level: BBa_K1486033
location: https://synbiohub.org/public/igem/BBa_K1486033/annotation2418786/range2418786/1
component: BBa_K1486025

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type: Range
persistent identity: range2418786
display id: range2418786
version: 1
direction: direction/reverse
top level: BBa_K1486033
start: 976
end: 1163
orientation: inline

BBa_K1486020
type: SequenceAnnotation
persistent identity: annotation2418784
display id: annotation2418784
version: 1
top level: BBa_K1486033
location: https://synbiohub.org/public/igem/BBa_K1486033/annotation2418784/range2418784/1
component: BBa_K1486020

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type: Range
persistent identity: range2418784
display id: range2418784
version: 1
direction: direction/reverse
top level: BBa_K1486033
start: 370
end: 975
orientation: inline

BBa_K1486019
type: SequenceAnnotation
persistent identity: annotation2418783
display id: annotation2418783
version: 1
top level: BBa_K1486033
location: https://synbiohub.org/public/igem/BBa_K1486033/annotation2418783/range2418783/1
component: BBa_K1486019

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type: Range
persistent identity: range2418783
display id: range2418783
version: 1
direction: direction/reverse
top level: BBa_K1486033
start: 40
end: 369
orientation: inline

BBa_K389011
type: ComponentDefinition
persistent identity: BBa K389011
display id: BBa_K389011
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: VirA screening device
creator: Jonas Aretz
created: 2010-08-09T11:00:00Z
modified: 2015-05-08T01:12:19Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _517_
m user id: 0
owner id: 6670
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This part contains: - a mutated ''virG'' response regulator from the virA/G receptor system from ''Agrobacterium tumefaciens'' which works in ''Escherichia coli'' without an ''rpoA'' gene from ''A. tumefaciens'' - a kanamycin resistance under the control of a ''vir'' promotor This device is used to screen ''virA'' receptor mutants. The ''virA'' receptor is mutated in the BBa_K389010 BioBrick. The better the receptor detects a substance, the more ''virG'' is phosphorylated and the stronger the kanamycin resistance from this BioBrick is expressed. The BioBricks BBa_K389010 and BBa_K389011 have to be used together in one cell.
mutable notes: medium strong constitutive promotor, terminator to keep basal expression of kanamycin resistance low
mutable provenance: synthetic ''virG'' gene, ''vir'' promotor from ''A. tumefaciens'' C58, partsregistry
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K389002
component: BBa_K389003
component: BBa_K389005
component: BBa_B0010
component: BBa_J23110
sequence annotation: BBa_B0010
sequence annotation: BBa_K389003
sequence annotation: BBa_J23110
sequence annotation: BBa_K389002
sequence annotation: BBa_K389005
sequence: https://synbiohub.org/public/igem/BBa_K389011_sequence/1

BBa_K389002
type: Component
persistent identity: component2078370
display id: component2078370
version: 1
top level: BBa_K389011
access: public
definition: https://synbiohub.org/public/igem/BBa_K389002/1

BBa_K389003
type: Component
persistent identity: component2078379
display id: component2078379
version: 1
top level: BBa_K389011
access: public
definition: https://synbiohub.org/public/igem/BBa_K389003/1

BBa_K389005
type: Component
persistent identity: component2078382
display id: component2078382
version: 1
top level: BBa_K389011
access: public
definition: kanR

BBa_B0010
type: Component
persistent identity: component2078373
display id: component2078373
version: 1
top level: BBa_K389011
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0010
type: Component
persistent identity: component2078371
display id: component2078371
version: 1
top level: BBa_K389011
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_J23110
type: Component
persistent identity: component2078366
display id: component2078366
version: 1
top level: BBa_K389011
access: public
definition: https://synbiohub.org/public/igem/BBa_J23110/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2078371
display id: annotation2078371
version: 1
top level: BBa_K389011
location: https://synbiohub.org/public/igem/BBa_K389011/annotation2078371/range2078371/1
component: BBa_B0010

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type: Range
persistent identity: range2078371
display id: range2078371
version: 1
direction: direction/forward
top level: BBa_K389011
start: 792
end: 871
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2078373
display id: annotation2078373
version: 1
top level: BBa_K389011
location: https://synbiohub.org/public/igem/BBa_K389011/annotation2078373/range2078373/1
component: BBa_B0010

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type: Range
persistent identity: range2078373
display id: range2078373
version: 1
direction: direction/forward
top level: BBa_K389011
start: 880
end: 959
orientation: inline

BBa_K389003
type: SequenceAnnotation
persistent identity: annotation2078379
display id: annotation2078379
version: 1
top level: BBa_K389011
location: https://synbiohub.org/public/igem/BBa_K389011/annotation2078379/range2078379/1
component: BBa_K389003

https://synbiohub.org/public/igem/BBa_K389011/annotation2078379/range2078379/1
type: Range
persistent identity: range2078379
display id: range2078379
version: 1
direction: direction/reverse
top level: BBa_K389011
start: 968
end: 1125
orientation: inline

BBa_J23110
type: SequenceAnnotation
persistent identity: annotation2078366
display id: annotation2078366
version: 1
top level: BBa_K389011
location: https://synbiohub.org/public/igem/BBa_K389011/annotation2078366/range2078366/1
component: BBa_J23110

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type: Range
persistent identity: range2078366
display id: range2078366
version: 1
direction: direction/reverse
top level: BBa_K389011
start: 1
end: 35
orientation: inline

BBa_K389002
type: SequenceAnnotation
persistent identity: annotation2078370
display id: annotation2078370
version: 1
top level: BBa_K389011
location: https://synbiohub.org/public/igem/BBa_K389011/annotation2078370/range2078370/1
component: BBa_K389002

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type: Range
persistent identity: range2078370
display id: range2078370
version: 1
direction: direction/reverse
top level: BBa_K389011
start: 44
end: 783
orientation: inline

BBa_K389005
type: SequenceAnnotation
persistent identity: annotation2078382
display id: annotation2078382
version: 1
top level: BBa_K389011
location: https://synbiohub.org/public/igem/BBa_K389011/annotation2078382/range2078382/1
component: BBa_K389005

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type: Range
persistent identity: range2078382
display id: range2078382
version: 1
direction: direction/reverse
top level: BBa_K389011
start: 1132
end: 1950
orientation: inline