//cds/transcriptionalregulator/activator
type: Collection
persistent identity: cds transcriptionalregulator activator
display id: cds_transcriptionalregulator_activator
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
owned by: james
owned by: myers
member: BBa_K1486013
member: BBa_K1709004
member: LuxO D47A
member: BBa_K1520013
member: VirA/G
member: BBa_K389413
member: BBa_K1031019
member: BBa_K2023010
member: BBa_K105001
member: BBa_K1315005
member: BBa_K1031442
member: BBa_K1486040
member: BBa_K1486010
member: BBa_K1031111
member: BBa_K108021
member: BBa_K1031913
member: BBa_K1946005
member: BBa_K1031912
member: BBa_K932000
member: BBa_K1031018
member: BBa_K1520501
member: rcsA
member: BBa_K1639002
member: BBa_K1486042
member: BBa_K389412
member: BBa_K1031444
member: BBa_K1486012
member: BBa_K1031311
member: BBa_K1520021
member: luxr
member: lasr
member: BBa_K1031441
member: VP64
member: BBa_K082006
member: BBa_K1031011
member: BBa_K352001
member: BBa_K606033
member: BBa_K1067008
member: BBa_K1486008
member: BBa_K1486001
member: cinr
member: BBa_K1486014
member: rhIR
member: BBa_K1031917
member: BBa_K1315000
member: BBa_K1946000
member: BBa_K294205
member: BBa_K389411
member: BBa_K2023012
member: BBa_J07009
member: BBa_K1031100
member: BBa_K1031410
member: BBa_K1486015
member: BBa_K1486041
member: BBa_K1486011
member: BBa_K266002
member: BBa_K2023015
member: BBa_K1709006
member: BBa_J120015
member: BBa_K1031911
member: BBa_K1072002
member: BBa_K1520024
member: BBa_K389011
member: BBa_K1031443
member: BBa_K1520023
member: BBa_K2036000
member: BBa_K1031112
member: BBa_K1031017
member: LuxO D47E
member: rhlR
member: virG

BBa_K1031011
type: ComponentDefinition
persistent identity: BBa K1031011
display id: BBa_K1031011
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: B0034-RiboJ-φR73
creator: He Shuaixin
created: 2013-09-08T11:00:00Z
modified: 2015-05-08T01:08:47Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1338_
m user id: 0
owner id: 15843
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: phiR-73
mutable notes: phiR73 phage
mutable provenance: lab made
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: phiR73
sequence: https://synbiohub.org/public/igem/BBa_K1031011_sequence/1

phiR73
type: SequenceAnnotation
persistent identity: annotation2353995
display id: annotation2353995
version: 1
top level: BBa_K1031011
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1031011/annotation2353995/range2353995/1

https://synbiohub.org/public/igem/BBa_K1031011/annotation2353995/range2353995/1
type: Range
persistent identity: range2353995
display id: range2353995
version: 1
direction: direction/forward
top level: BBa_K1031011
start: 94
end: 339
orientation: inline

BBa_K748000
type: ComponentDefinition
persistent identity: BBa K748000
display id: BBa_K748000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: AgrA protein coding sequence. AgrA is the main component of S.aureus agr quorum sensing system.
creator: Lei Qiao
created: 2012-09-14T11:00:00Z
modified: 2015-05-08T01:13:12Z
discontinued: false
dominant: false
experience: experience/None
group u list: _999_
m user id: 0
owner id: 8564
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Staphylococcus aureus AgrA, the transcriptional component of a quorum sensing system and global regulator of virulence that up-regulates secreted virulence factors and down-regulates cell wall-associated proteins, can bind in both the P2 and P3 promoter regions of the agr locus. The composite of AgrC and AIP then leads to phosphorylation of AgrA. AgrA in its phosphorylated sate activates transcription from both P2 and P3, leading to the production of GFP and 3OC6HSL. Thus we can detect the presence of S.aureus expediently by observing the expression of GFP.
mutable notes: As a AIP sensor system, agrA should work with agrC.
mutable provenance: Staphylococcus aureus genomic sequence
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K748000_sequence/1

BBa_K1486043
type: ComponentDefinition
persistent identity: BBa K1486043
display id: BBa_K1486043
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Leucine Zipper + split rLuc
creator: EPFL iGem team 2014
created: 2014-10-01T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: To edit. Pense ?? rajouter les linkers, je ne sais pas s'ils sont de igem ou s'il faut ajouter la s??quence ?? la registry. Check pour tous tes biobricks et dis-moi, please.
mutable notes: To edit
mutable provenance: To edit
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_I0500
component: BBa_K1486042
component: BBa_B0034
component: BBa_K1486019
component: BBa_K1486020
sequence annotation: BBa_K1486020
sequence annotation: BBa_K1486042
sequence annotation: BBa_K1486019
sequence annotation: BBa_B0034
sequence annotation: BBa_I0500
sequence: https://synbiohub.org/public/igem/BBa_K1486043_sequence/1

BBa_I0500
type: Component
persistent identity: component2392170
display id: component2392170
version: 1
top level: BBa_K1486043
access: public
definition: https://synbiohub.org/public/igem/BBa_I0500/1

BBa_K1486042
type: Component
persistent identity: component2392173
display id: component2392173
version: 1
top level: BBa_K1486043
access: public
definition: BBa_K1486042

BBa_B0034
type: Component
persistent identity: component2392176
display id: component2392176
version: 1
top level: BBa_K1486043
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K1486042
type: Component
persistent identity: component2392177
display id: component2392177
version: 1
top level: BBa_K1486043
access: public
definition: BBa_K1486042

BBa_K1486019
type: Component
persistent identity: component2392174
display id: component2392174
version: 1
top level: BBa_K1486043
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486019/1

BBa_K1486020
type: Component
persistent identity: component2392178
display id: component2392178
version: 1
top level: BBa_K1486043
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486020/1

BBa_B0034
type: Component
persistent identity: component2392172
display id: component2392172
version: 1
top level: BBa_K1486043
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K1486020
type: SequenceAnnotation
persistent identity: annotation2392178
display id: annotation2392178
version: 1
top level: BBa_K1486043
location: https://synbiohub.org/public/igem/BBa_K1486043/annotation2392178/range2392178/1
component: BBa_K1486020

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type: Range
persistent identity: range2392178
display id: range2392178
version: 1
direction: direction/reverse
top level: BBa_K1486043
start: 1865
end: 2470
orientation: inline

BBa_K1486042
type: SequenceAnnotation
persistent identity: annotation2392173
display id: annotation2392173
version: 1
top level: BBa_K1486043
location: https://synbiohub.org/public/igem/BBa_K1486043/annotation2392173/range2392173/1
component: BBa_K1486042

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type: Range
persistent identity: range2392173
display id: range2392173
version: 1
direction: direction/reverse
top level: BBa_K1486043
start: 1223
end: 1372
orientation: inline

BBa_K1486019
type: SequenceAnnotation
persistent identity: annotation2392174
display id: annotation2392174
version: 1
top level: BBa_K1486043
location: https://synbiohub.org/public/igem/BBa_K1486043/annotation2392174/range2392174/1
component: BBa_K1486019

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type: Range
persistent identity: range2392174
display id: range2392174
version: 1
direction: direction/reverse
top level: BBa_K1486043
start: 1373
end: 1702
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2392172
display id: annotation2392172
version: 1
top level: BBa_K1486043
location: https://synbiohub.org/public/igem/BBa_K1486043/annotation2392172/range2392172/1
component: BBa_B0034

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type: Range
persistent identity: range2392172
display id: range2392172
version: 1
direction: direction/reverse
top level: BBa_K1486043
start: 1211
end: 1222
orientation: inline

BBa_I0500
type: SequenceAnnotation
persistent identity: annotation2392170
display id: annotation2392170
version: 1
top level: BBa_K1486043
location: https://synbiohub.org/public/igem/BBa_K1486043/annotation2392170/range2392170/1
component: BBa_I0500

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type: Range
persistent identity: range2392170
display id: range2392170
version: 1
direction: direction/reverse
top level: BBa_K1486043
start: 1
end: 1210
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2392176
display id: annotation2392176
version: 1
top level: BBa_K1486043
location: https://synbiohub.org/public/igem/BBa_K1486043/annotation2392176/range2392176/1
component: BBa_B0034

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type: Range
persistent identity: range2392176
display id: range2392176
version: 1
direction: direction/reverse
top level: BBa_K1486043
start: 1703
end: 1714
orientation: inline

BBa_K1486042
type: SequenceAnnotation
persistent identity: annotation2392177
display id: annotation2392177
version: 1
top level: BBa_K1486043
location: https://synbiohub.org/public/igem/BBa_K1486043/annotation2392177/range2392177/1
component: BBa_K1486042

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type: Range
persistent identity: range2392177
display id: range2392177
version: 1
direction: direction/reverse
top level: BBa_K1486043
start: 1715
end: 1864
orientation: inline

BBa_K1067007
type: ComponentDefinition
persistent identity: BBa K1067007
display id: BBa_K1067007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Tight pBAD with araC
creator: Kristian Davidsen and Helen Cook
created: 2013-09-27T11:00:00Z
modified: 2015-05-08T01:09:00Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1376_
m user id: 0
owner id: 16028
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: ggg
mutable notes: ggg
mutable provenance: ggg
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: araC
sequence annotation: araO2
sequence annotation: araI2
sequence annotation: araO1L
sequence annotation: Tight pBAD promoter
sequence annotation: -10
sequence annotation: araI1
sequence annotation: araO1R
sequence annotation: CRP binding site
sequence annotation: -35
sequence: https://synbiohub.org/public/igem/BBa_K1067007_sequence/1

araC
type: SequenceAnnotation
persistent identity: annotation2366479
display id: annotation2366479
version: 1
top level: BBa_K1067007
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1067007/annotation2366479/range2366479/1

https://synbiohub.org/public/igem/BBa_K1067007/annotation2366479/range2366479/1
type: Range
persistent identity: range2366479
display id: range2366479
version: 1
direction: direction/forward
top level: BBa_K1067007
start: 1
end: 880
orientation: inline

araO2
type: SequenceAnnotation
persistent identity: annotation2366480
display id: annotation2366480
version: 1
top level: BBa_K1067007
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1067007/annotation2366480/range2366480/1

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type: Range
persistent identity: range2366480
display id: range2366480
version: 1
direction: direction/forward
top level: BBa_K1067007
start: 908
end: 924
orientation: inline

araI2
type: SequenceAnnotation
persistent identity: annotation2366485
display id: annotation2366485
version: 1
top level: BBa_K1067007
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1067007/annotation2366485/range2366485/1

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type: Range
persistent identity: range2366485
display id: range2366485
version: 1
direction: direction/forward
top level: BBa_K1067007
start: 1140
end: 1157
orientation: inline

araO1L
type: SequenceAnnotation
persistent identity: annotation2366481
display id: annotation2366481
version: 1
top level: BBa_K1067007
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1067007/annotation2366481/range2366481/1

https://synbiohub.org/public/igem/BBa_K1067007/annotation2366481/range2366481/1
type: Range
persistent identity: range2366481
display id: range2366481
version: 1
direction: direction/forward
top level: BBa_K1067007
start: 1045
end: 1061
orientation: inline

Tight pBAD promoter
type: SequenceAnnotation
persistent identity: annotation2366486
display id: annotation2366486
version: 1
top level: BBa_K1067007
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1067007/annotation2366486/range2366486/1

https://synbiohub.org/public/igem/BBa_K1067007/annotation2366486/range2366486/1
type: Range
persistent identity: range2366486
display id: range2366486
version: 1
direction: direction/forward
top level: BBa_K1067007
start: 1153
end: 1189
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2366488
display id: annotation2366488
version: 1
top level: BBa_K1067007
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1067007/annotation2366488/range2366488/1

https://synbiohub.org/public/igem/BBa_K1067007/annotation2366488/range2366488/1
type: Range
persistent identity: range2366488
display id: range2366488
version: 1
direction: direction/forward
top level: BBa_K1067007
start: 1177
end: 1182
orientation: inline

araI1
type: SequenceAnnotation
persistent identity: annotation2366484
display id: annotation2366484
version: 1
top level: BBa_K1067007
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1067007/annotation2366484/range2366484/1

https://synbiohub.org/public/igem/BBa_K1067007/annotation2366484/range2366484/1
type: Range
persistent identity: range2366484
display id: range2366484
version: 1
direction: direction/forward
top level: BBa_K1067007
start: 1119
end: 1135
orientation: inline

araO1R
type: SequenceAnnotation
persistent identity: annotation2366482
display id: annotation2366482
version: 1
top level: BBa_K1067007
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1067007/annotation2366482/range2366482/1

https://synbiohub.org/public/igem/BBa_K1067007/annotation2366482/range2366482/1
type: Range
persistent identity: range2366482
display id: range2366482
version: 1
direction: direction/forward
top level: BBa_K1067007
start: 1066
end: 1082
orientation: inline

CRP binding site
type: SequenceAnnotation
persistent identity: annotation2366483
display id: annotation2366483
version: 1
top level: BBa_K1067007
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1067007/annotation2366483/range2366483/1

https://synbiohub.org/public/igem/BBa_K1067007/annotation2366483/range2366483/1
type: Range
persistent identity: range2366483
display id: range2366483
version: 1
direction: direction/forward
top level: BBa_K1067007
start: 1087
end: 1108
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2366487
display id: annotation2366487
version: 1
top level: BBa_K1067007
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1067007/annotation2366487/range2366487/1

https://synbiohub.org/public/igem/BBa_K1067007/annotation2366487/range2366487/1
type: Range
persistent identity: range2366487
display id: range2366487
version: 1
direction: direction/forward
top level: BBa_K1067007
start: 1153
end: 1158
orientation: inline

araC
type: ComponentDefinition
persistent identity: BBa C0080
display id: BBa_C0080
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: araC arabinose operon regulatory protein (repressor/activator) from E. coli (+LVA)
creator: Sara Neves (Fighting Darwins)
created: 2004-01-27T12:00:00Z
modified: 2015-08-31T04:07:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: coding region for the araC gene, used to make araC protein for use in positive or negative regulation of TIPs output (see also R0080, R0081)
mutable provenance: GenBank: NC_002655 (www.ncbi.nlm.nih.gov)
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: LVA
sequence annotation: araC
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C0080_sequence/1

LVA
type: SequenceAnnotation
persistent identity: annotation308196
display id: annotation308196
version: 1
top level: araC
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_C0080/annotation308196/range308196/1

https://synbiohub.org/public/igem/BBa_C0080/annotation308196/range308196/1
type: Range
persistent identity: range308196
display id: range308196
version: 1
direction: direction/forward
top level: araC
start: 877
end: 909
orientation: inline

araC
type: SequenceAnnotation
persistent identity: annotation308191
display id: annotation308191
version: 1
top level: araC
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_C0080/annotation308191/range308191/1

https://synbiohub.org/public/igem/BBa_C0080/annotation308191/range308191/1
type: Range
persistent identity: range308191
display id: range308191
version: 1
direction: direction/forward
top level: araC
start: 1
end: 876
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2214004
display id: annotation2214004
version: 1
top level: araC
role: SO:0000807
role: feature/barcode
location: https://synbiohub.org/public/igem/BBa_C0080/annotation2214004/range2214004/1

https://synbiohub.org/public/igem/BBa_C0080/annotation2214004/range2214004/1
type: Range
persistent identity: range2214004
display id: range2214004
version: 1
direction: direction/forward
top level: araC
start: 916
end: 940
orientation: inline

BBa_K352001
type: ComponentDefinition
persistent identity: BBa K352001
display id: BBa_K352001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CooA from Rhodospirillum rubrum
creator: Cihan Tastan
created: 2010-10-01T11:00:00Z
modified: 2015-05-08T01:12:11Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _474_606_
m user id: 0
owner id: 5815
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: CooA is a heme-containing transcriptional activator that enables Rhodospirillum rubrum to sense and grow on CO as a sole energy source. CooA is a member of a family of transcriptional regulators similar to the cAMP receptor protein and fumavate nitrate reduction from Escherichia coli. The protein is active in sequence-specific DNA binding in the presence of CO, but not in the absence of CO. The protein to be a dimer in the absence of CO. The product, CooA, is 28% identical (51% similar) to CRP(cAMP receptor protein) and 18% identical (45% similar) to FNR(fumavate nitrate reduction) from Escherichia coli. Inactive Fe(II) CooA structure adapted from that of the strain with PDB identification no. 1FT9. The protein consists of two monomers, shaded differently, which dimerize along the central C-helices of adjacent effector-binding domains. The solved structure is asymmetric, in which one monomer contains fused C- and D-helices. Nonetheless, both F-helices that interact with DNA in a sequence-specific manner are buried from the surface in the structure. The 4/5 loop is noted and so are the Pro2 and His77 heme Fe(II) ligands.
mutable notes: The sequence information was acquired from NCBI and physical DNA was synthesized from GENEART. Classical cloning strategies(restriction digestion) were used to produce biobricks.
mutable provenance: Hwan Youn, Robert L. Kerby, Mary Conrad, et al. 2004. Functionally Critical Elements of CooA-Related CO Sensors. J. Bacteriol. 186(5):1320-1329. doi:10.1128/JB.186.5.1320-1329.2004.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Start Codon
sequence: https://synbiohub.org/public/igem/BBa_K352001_sequence/1

Start Codon
type: SequenceAnnotation
persistent identity: annotation2081251
display id: annotation2081251
version: 1
top level: BBa_K352001
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K352001/annotation2081251/range2081251/1

https://synbiohub.org/public/igem/BBa_K352001/annotation2081251/range2081251/1
type: Range
persistent identity: range2081251
display id: range2081251
version: 1
direction: direction/forward
top level: BBa_K352001
start: 1
end: 3
orientation: inline

BBa_K932000
type: ComponentDefinition
persistent identity: BBa K932000
display id: BBa_K932000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: OmpR234 under Tet Repressible Promoter
creator: Sean Kearney
created: 2012-09-15T11:00:00Z
modified: 2015-05-08T01:13:47Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1197_
m user id: 0
owner id: 6891
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part promotes the constitutive expression of the curli cluster (csgD), proteins involved in cell-cell adhesion.
mutable notes: This part regulates curli expression within the cell via transcriptional activation. The construct can be repressed in the presence of the TetR repressor.
mutable provenance: Comes from the E. coli K-12 genome.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K932001
component: BBa_B0015
component: BBa_R0040
sequence annotation: BBa_K932001
sequence annotation: BBa_B0015
sequence annotation: BBa_R0040
sequence: https://synbiohub.org/public/igem/BBa_K932000_sequence/1

BBa_K932001
type: Component
persistent identity: component2210130
display id: component2210130
version: 1
top level: BBa_K932000
access: public
definition: https://synbiohub.org/public/igem/BBa_K932001/1

BBa_B0015
type: Component
persistent identity: component2210137
display id: component2210137
version: 1
top level: BBa_K932000
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_R0040
type: Component
persistent identity: component2210124
display id: component2210124
version: 1
top level: BBa_K932000
access: public
definition: https://synbiohub.org/public/igem/BBa_R0040/1

BBa_K932001
type: SequenceAnnotation
persistent identity: annotation2210130
display id: annotation2210130
version: 1
top level: BBa_K932000
location: https://synbiohub.org/public/igem/BBa_K932000/annotation2210130/range2210130/1
component: BBa_K932001

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type: Range
persistent identity: range2210130
display id: range2210130
version: 1
direction: direction/reverse
top level: BBa_K932000
start: 61
end: 780
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2210137
display id: annotation2210137
version: 1
top level: BBa_K932000
location: https://synbiohub.org/public/igem/BBa_K932000/annotation2210137/range2210137/1
component: BBa_B0015

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type: Range
persistent identity: range2210137
display id: range2210137
version: 1
direction: direction/reverse
top level: BBa_K932000
start: 789
end: 917
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation2210124
display id: annotation2210124
version: 1
top level: BBa_K932000
location: https://synbiohub.org/public/igem/BBa_K932000/annotation2210124/range2210124/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_K932000/annotation2210124/range2210124/1
type: Range
persistent identity: range2210124
display id: range2210124
version: 1
direction: direction/forward
top level: BBa_K932000
start: 1
end: 54
orientation: inline

BBa_K1895007
type: ComponentDefinition
persistent identity: BBa K1895007
display id: BBa_K1895007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Sequence for sigma 32 transcription factor
creator: Jake Burton
created: 2016-10-17T11:00:00Z
modified: 2016-10-18T02:13:40Z
discontinued: false
dominant: false
group u list: _2361_
m user id: 29859
owner id: 29859
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This part is a correction to [http://parts.igem.org/Part:BBa_M36888 BBa_M36888] which has an illegal PstI site which makes it incompatible with BioBricks / RFC10. iGEM Teams can use this alternative sequence which contains none of the BioBrick restriction enzyme sites. Amino acid No. 202, Glysine has been mutated from CAG to CAA to remove the PstI site. The remainder of the sequence remains unchanged.
mutable notes: This part was checked for BioBrick restriction enzyme sites which would otherwise make it unsuitable for use by iGEM teams.
mutable provenance: This part is a correction to [http://parts.igem.org/Part:BBa_M36888 BBa_M36888].
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1895007_sequence/1

BBa_K1072002
type: ComponentDefinition
persistent identity: BBa K1072002
display id: BBa_K1072002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: NADH dehydrogenase
creator: Yichong wang, Ruichen, Xihao liao and Junjie zhang
created: 2013-08-31T11:00:00Z
modified: 2015-05-08T01:09:01Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1381_
m user id: 0
owner id: 16002
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: BBa_K1072002 is the generator fo NADH dehydrogenase which is an enzyme following chemical reaction: NADH + H+ acceptor \rightleftharpoons NAD+ + reduced acceptor
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0034
component: BBa_R0062
component: BBa_K1072001
component: BBa_B0010
component: BBa_B0012
sequence annotation: BBa_K1072001
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_R0062
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K1072002_sequence/1

BBa_B0034
type: Component
persistent identity: component2334350
display id: component2334350
version: 1
top level: BBa_K1072002
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_R0062
type: Component
persistent identity: component2334344
display id: component2334344
version: 1
top level: BBa_K1072002
access: public
definition: https://synbiohub.org/public/igem/BBa_R0062/1

BBa_K1072001
type: Component
persistent identity: component2334352
display id: component2334352
version: 1
top level: BBa_K1072002
access: public
definition: https://synbiohub.org/public/igem/BBa_K1072001/1

BBa_B0010
type: Component
persistent identity: component2334353
display id: component2334353
version: 1
top level: BBa_K1072002
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0012
type: Component
persistent identity: component2334355
display id: component2334355
version: 1
top level: BBa_K1072002
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_K1072001
type: SequenceAnnotation
persistent identity: annotation2334352
display id: annotation2334352
version: 1
top level: BBa_K1072002
location: https://synbiohub.org/public/igem/BBa_K1072002/annotation2334352/range2334352/1
component: BBa_K1072001

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type: Range
persistent identity: range2334352
display id: range2334352
version: 1
direction: direction/reverse
top level: BBa_K1072002
start: 84
end: 1388
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2334353
display id: annotation2334353
version: 1
top level: BBa_K1072002
location: https://synbiohub.org/public/igem/BBa_K1072002/annotation2334353/range2334353/1
component: BBa_B0010

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type: Range
persistent identity: range2334353
display id: range2334353
version: 1
direction: direction/forward
top level: BBa_K1072002
start: 1397
end: 1476
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2334355
display id: annotation2334355
version: 1
top level: BBa_K1072002
location: https://synbiohub.org/public/igem/BBa_K1072002/annotation2334355/range2334355/1
component: BBa_B0012

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type: Range
persistent identity: range2334355
display id: range2334355
version: 1
direction: direction/forward
top level: BBa_K1072002
start: 1485
end: 1525
orientation: inline

BBa_R0062
type: SequenceAnnotation
persistent identity: annotation2334344
display id: annotation2334344
version: 1
top level: BBa_K1072002
location: https://synbiohub.org/public/igem/BBa_K1072002/annotation2334344/range2334344/1
component: BBa_R0062

https://synbiohub.org/public/igem/BBa_K1072002/annotation2334344/range2334344/1
type: Range
persistent identity: range2334344
display id: range2334344
version: 1
direction: direction/forward
top level: BBa_K1072002
start: 1
end: 55
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2334350
display id: annotation2334350
version: 1
top level: BBa_K1072002
location: https://synbiohub.org/public/igem/BBa_K1072002/annotation2334350/range2334350/1
component: BBa_B0034

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type: Range
persistent identity: range2334350
display id: range2334350
version: 1
direction: direction/reverse
top level: BBa_K1072002
start: 64
end: 75
orientation: inline

BBa_K1486040
type: ComponentDefinition
persistent identity: BBa K1486040
display id: BBa_K1486040
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: sfGFPN + CpxR
creator: EPFL iGem team 2014
created: 2014-09-26T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: To edit
mutable notes: To edit
mutable provenance: To edit
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K1486038
component: BBa_B0034
component: BBa_I0500
component: BBa_K1486037
component: BBa_K1486000
sequence annotation: BBa_K1486038
sequence annotation: BBa_I0500
sequence annotation: BBa_K1486000
sequence annotation: BBa_K1486037
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K1486040_sequence/1

BBa_K1486038
type: Component
persistent identity: component2387188
display id: component2387188
version: 1
top level: BBa_K1486040
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486038/1

BBa_B0034
type: Component
persistent identity: component2387187
display id: component2387187
version: 1
top level: BBa_K1486040
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_I0500
type: Component
persistent identity: component2387185
display id: component2387185
version: 1
top level: BBa_K1486040
access: public
definition: https://synbiohub.org/public/igem/BBa_I0500/1

BBa_K1486037
type: Component
persistent identity: component2387189
display id: component2387189
version: 1
top level: BBa_K1486040
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486037/1

BBa_K1486000
type: Component
persistent identity: component2387193
display id: component2387193
version: 1
top level: BBa_K1486040
access: public
definition: BBa_K1486000

BBa_K1486038
type: SequenceAnnotation
persistent identity: annotation2387188
display id: annotation2387188
version: 1
top level: BBa_K1486040
location: https://synbiohub.org/public/igem/BBa_K1486040/annotation2387188/range2387188/1
component: BBa_K1486038

https://synbiohub.org/public/igem/BBa_K1486040/annotation2387188/range2387188/1
type: Range
persistent identity: range2387188
display id: range2387188
version: 1
direction: direction/reverse
top level: BBa_K1486040
start: 1223
end: 1864
orientation: inline

BBa_I0500
type: SequenceAnnotation
persistent identity: annotation2387185
display id: annotation2387185
version: 1
top level: BBa_K1486040
location: https://synbiohub.org/public/igem/BBa_K1486040/annotation2387185/range2387185/1
component: BBa_I0500

https://synbiohub.org/public/igem/BBa_K1486040/annotation2387185/range2387185/1
type: Range
persistent identity: range2387185
display id: range2387185
version: 1
direction: direction/reverse
top level: BBa_K1486040
start: 1
end: 1210
orientation: inline

BBa_K1486000
type: SequenceAnnotation
persistent identity: annotation2387193
display id: annotation2387193
version: 1
top level: BBa_K1486040
location: https://synbiohub.org/public/igem/BBa_K1486040/annotation2387193/range2387193/1
component: BBa_K1486000

https://synbiohub.org/public/igem/BBa_K1486040/annotation2387193/range2387193/1
type: Range
persistent identity: range2387193
display id: range2387193
version: 1
direction: direction/reverse
top level: BBa_K1486040
start: 1904
end: 2605
orientation: inline

BBa_K1486037
type: SequenceAnnotation
persistent identity: annotation2387189
display id: annotation2387189
version: 1
top level: BBa_K1486040
location: https://synbiohub.org/public/igem/BBa_K1486040/annotation2387189/range2387189/1
component: BBa_K1486037

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type: Range
persistent identity: range2387189
display id: range2387189
version: 1
direction: direction/reverse
top level: BBa_K1486040
start: 1865
end: 1903
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2387187
display id: annotation2387187
version: 1
top level: BBa_K1486040
location: https://synbiohub.org/public/igem/BBa_K1486040/annotation2387187/range2387187/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1486040/annotation2387187/range2387187/1
type: Range
persistent identity: range2387187
display id: range2387187
version: 1
direction: direction/reverse
top level: BBa_K1486040
start: 1211
end: 1222
orientation: inline

BBa_K2036000
type: ComponentDefinition
persistent identity: BBa K2036000
display id: BBa_K2036000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CII bacteriophage lambda trascriptional activator
creator: Zhangyu Cheng
created: 2016-09-17T11:00:00Z
modified: 2016-09-27T09:08:58Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2503_
m user id: 25901
owner id: 25901
owning group id: 9
status: status/Planning
bookmark: false
mutable description: CII is an important part of bacteriaphage lambda operon.
mutable notes: CII is an transcriptional activator,it can
mutable provenance: come from bacteriaphage lambda
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: RBS core sequence
sequence annotation: Start codon
sequence: https://synbiohub.org/public/igem/BBa_K2036000_sequence/1

RBS core sequence
type: SequenceAnnotation
persistent identity: annotation2484232
display id: annotation2484232
version: 1
top level: BBa_K2036000
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K2036000/annotation2484232/range2484232/1

https://synbiohub.org/public/igem/BBa_K2036000/annotation2484232/range2484232/1
type: Range
persistent identity: range2484232
display id: range2484232
version: 1
direction: direction/forward
top level: BBa_K2036000
start: 1
end: 12
orientation: inline

Start codon
type: SequenceAnnotation
persistent identity: annotation2484233
display id: annotation2484233
version: 1
top level: BBa_K2036000
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K2036000/annotation2484233/range2484233/1

https://synbiohub.org/public/igem/BBa_K2036000/annotation2484233/range2484233/1
type: Range
persistent identity: range2484233
display id: range2484233
version: 1
direction: direction/forward
top level: BBa_K2036000
start: 19
end: 21
orientation: inline

BBa_K1486009
type: ComponentDefinition
persistent identity: BBa K1486009
display id: BBa_K1486009
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CxpR & Split IFP1.4 [Nterm + Nterm]
creator: iGem EPFL 2014
created: 2014-08-26T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part has two fused proteins downstream of an arabinose-inducible promoter: CpxR-IFP[1] and CpxR-IFP[2]. This construct was made in all orientations: - CpxR-IFP[1] + CpxR-IFP[2] (BBa_K1286008) - IFP[1]-CpxR + CpxR-IFP[2] (BBa_K1486010) - CpxR-IFP[1] + IFP[2]-CpxR (BBa_K1486011)
mutable notes: All orientations were made, so all dimerizations could be tested (see main page for biobrick numbers). Flexible linkers were used for each of the fused proteins, and an RBS was put before each one of them.
mutable provenance: From Michnick Article. To Edit
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K1486004
component: BBa_K1486007
component: BBa_I0500
component: BBa_K1486000
component: BBa_K1486052
component: BBa_B0034
component: BBa_K1486006
sequence annotation: BBa_K1486000
sequence annotation: BBa_B0034
sequence annotation: BBa_K1486052
sequence annotation: BBa_K1486004
sequence annotation: BBa_K1486006
sequence annotation: BBa_I0500
sequence annotation: BBa_K1486007
sequence: https://synbiohub.org/public/igem/BBa_K1486009_sequence/1

BBa_K1486004
type: Component
persistent identity: component2420784
display id: component2420784
version: 1
top level: BBa_K1486009
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486004/1

BBa_K1486007
type: Component
persistent identity: component2420756
display id: component2420756
version: 1
top level: BBa_K1486009
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486007/1

BBa_I0500
type: Component
persistent identity: component2420763
display id: component2420763
version: 1
top level: BBa_K1486009
access: public
definition: https://synbiohub.org/public/igem/BBa_I0500/1

BBa_K1486004
type: Component
persistent identity: component2420772
display id: component2420772
version: 1
top level: BBa_K1486009
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486004/1

BBa_K1486000
type: Component
persistent identity: component2420776
display id: component2420776
version: 1
top level: BBa_K1486009
access: public
definition: BBa_K1486000

BBa_K1486052
type: Component
persistent identity: component2420777
display id: component2420777
version: 1
top level: BBa_K1486009
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486052/1

BBa_K1486000
type: Component
persistent identity: component2420788
display id: component2420788
version: 1
top level: BBa_K1486009
access: public
definition: BBa_K1486000

BBa_B0034
type: Component
persistent identity: component2420767
display id: component2420767
version: 1
top level: BBa_K1486009
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K1486004
type: Component
persistent identity: component2420758
display id: component2420758
version: 1
top level: BBa_K1486009
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486004/1

BBa_K1486000
type: Component
persistent identity: component2420766
display id: component2420766
version: 1
top level: BBa_K1486009
access: public
definition: BBa_K1486000

BBa_K1486007
type: Component
persistent identity: component2420783
display id: component2420783
version: 1
top level: BBa_K1486009
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486007/1

BBa_B0034
type: Component
persistent identity: component2420779
display id: component2420779
version: 1
top level: BBa_K1486009
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K1486006
type: Component
persistent identity: component2420771
display id: component2420771
version: 1
top level: BBa_K1486009
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486006/1

BBa_K1486000
type: SequenceAnnotation
persistent identity: annotation2420766
display id: annotation2420766
version: 1
top level: BBa_K1486009
location: https://synbiohub.org/public/igem/BBa_K1486009/annotation2420766/range2420766/1
component: BBa_K1486000

https://synbiohub.org/public/igem/BBa_K1486009/annotation2420766/range2420766/1
type: Range
persistent identity: range2420766
display id: range2420766
version: 1
direction: direction/reverse
top level: BBa_K1486009
start: 3025
end: 3726
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2420767
display id: annotation2420767
version: 1
top level: BBa_K1486009
location: https://synbiohub.org/public/igem/BBa_K1486009/annotation2420767/range2420767/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1486009/annotation2420767/range2420767/1
type: Range
persistent identity: range2420767
display id: range2420767
version: 1
direction: direction/reverse
top level: BBa_K1486009
start: 1211
end: 1222
orientation: inline

BBa_K1486052
type: SequenceAnnotation
persistent identity: annotation2420777
display id: annotation2420777
version: 1
top level: BBa_K1486009
location: https://synbiohub.org/public/igem/BBa_K1486009/annotation2420777/range2420777/1
component: BBa_K1486052

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type: Range
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BBa_K1486000
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BBa_K1486004
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persistent identity: annotation2420784
display id: annotation2420784
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BBa_K1486006
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persistent identity: annotation2420771
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BBa_K1486000
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persistent identity: annotation2420776
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persistent identity: range2420776
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BBa_I0500
type: SequenceAnnotation
persistent identity: annotation2420763
display id: annotation2420763
version: 1
top level: BBa_K1486009
location: https://synbiohub.org/public/igem/BBa_K1486009/annotation2420763/range2420763/1
component: BBa_I0500

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persistent identity: range2420763
display id: range2420763
version: 1
direction: direction/reverse
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BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2420779
display id: annotation2420779
version: 1
top level: BBa_K1486009
location: https://synbiohub.org/public/igem/BBa_K1486009/annotation2420779/range2420779/1
component: BBa_B0034

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type: Range
persistent identity: range2420779
display id: range2420779
version: 1
direction: direction/reverse
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start: 2398
end: 2409
orientation: inline

BBa_K1486007
type: SequenceAnnotation
persistent identity: annotation2420783
display id: annotation2420783
version: 1
top level: BBa_K1486009
location: https://synbiohub.org/public/igem/BBa_K1486009/annotation2420783/range2420783/1
component: BBa_K1486007

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type: Range
persistent identity: range2420783
display id: range2420783
version: 1
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start: 2410
end: 2994
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BBa_K1486004
type: SequenceAnnotation
persistent identity: annotation2420772
display id: annotation2420772
version: 1
top level: BBa_K1486009
location: https://synbiohub.org/public/igem/BBa_K1486009/annotation2420772/range2420772/1
component: BBa_K1486004

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persistent identity: range2420772
display id: range2420772
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BBa_K1486004
type: SequenceAnnotation
persistent identity: annotation2420758
display id: annotation2420758
version: 1
top level: BBa_K1486009
location: https://synbiohub.org/public/igem/BBa_K1486009/annotation2420758/range2420758/1
component: BBa_K1486004

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persistent identity: range2420758
display id: range2420758
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end: 3024
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BBa_K1486007
type: SequenceAnnotation
persistent identity: annotation2420756
display id: annotation2420756
version: 1
top level: BBa_K1486009
location: https://synbiohub.org/public/igem/BBa_K1486009/annotation2420756/range2420756/1
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display id: range2420756
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BBa_K1031912
type: ComponentDefinition
persistent identity: BBa K1031912
display id: BBa_K1031912
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: J23105-XylS-Terminator
creator: He Shuaixin
created: 2013-09-08T11:00:00Z
modified: 2015-05-08T01:08:48Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1338_
m user id: 0
owner id: 15843
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: 105-XylS-TT
mutable notes: molecular cloning
mutable provenance: synthesis Pseudomonas putida
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: B0015
sequence annotation: XylS
sequence annotation: J23105
sequence: https://synbiohub.org/public/igem/BBa_K1031912_sequence/1

B0015
type: SequenceAnnotation
persistent identity: annotation2360108
display id: annotation2360108
version: 1
top level: BBa_K1031912
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1031912/annotation2360108/range2360108/1

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type: Range
persistent identity: range2360108
display id: range2360108
version: 1
direction: direction/forward
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start: 1034
end: 1162
orientation: inline

XylS
type: SequenceAnnotation
persistent identity: annotation2360107
display id: annotation2360107
version: 1
top level: BBa_K1031912
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1031912/annotation2360107/range2360107/1

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persistent identity: range2360107
display id: range2360107
version: 1
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start: 62
end: 1028
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J23105
type: SequenceAnnotation
persistent identity: annotation2360063
display id: annotation2360063
version: 1
top level: BBa_K1031912
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1031912/annotation2360063/range2360063/1

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type: Range
persistent identity: range2360063
display id: range2360063
version: 1
direction: direction/forward
top level: BBa_K1031912
start: 1
end: 35
orientation: inline

BBa_K1709006
type: ComponentDefinition
persistent identity: BBa K1709006
display id: BBa_K1709006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS-LuxI-His-RBS-LuxR-E-Term-Plux
creator: Astrid Deryckere
created: 2015-09-17T11:00:00Z
modified: 2015-09-18T11:29:08Z
discontinued: false
dominant: false
group u list: _2129_
m user id: 25389
owner id: 25389
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Autoinducer synthetase for acyl-homoserine lactones (AHL): LuxI synthesizes AHL (3OC6HSL). Two molecules of AHL form a complex with two molecules of the LuxR protein. This complex then binds to a palyndromic site on the lux promoter, increasing its transcription rate. LuxI, LuxR and the right lux promoter are all derived from V. fischeri. In absence of AHL, the right Lux promotor only gives weak constitutive expression of downstream genes. Any gene you would like to be controlled by AHL and thus cell density can be cloned after this complex.
mutable notes: For the ease of cloning, the sequence was devided in multiple gBlocks. Therefor, there is a mistake in the composite part sequence since the scar is only partly present.
mutable provenance: The Lux operon originates from V. Fischeri.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
component: BBa_K1709001
component: BBa_B1002
component: BBa_R0062
component: BBa_K1709004
component: BBa_J61100
sequence annotation: BBa_J61100
sequence annotation: BBa_B1002
sequence annotation: BBa_R0062
sequence annotation: BBa_K1709004
sequence annotation: BBa_K1709001
sequence: https://synbiohub.org/public/igem/BBa_K1709006_sequence/1

BBa_K1709001
type: Component
persistent identity: component2473330
display id: component2473330
version: 1
top level: BBa_K1709006
access: public
definition: https://synbiohub.org/public/igem/BBa_K1709001/1

BBa_B1002
type: Component
persistent identity: component2473341
display id: component2473341
version: 1
top level: BBa_K1709006
access: public
definition: https://synbiohub.org/public/igem/BBa_B1002/1

BBa_R0062
type: Component
persistent identity: component2473343
display id: component2473343
version: 1
top level: BBa_K1709006
access: public
definition: https://synbiohub.org/public/igem/BBa_R0062/1

BBa_K1709004
type: Component
persistent identity: component2473336
display id: component2473336
version: 1
top level: BBa_K1709006
access: public
definition: BBa_K1709004

BBa_J61100
type: Component
persistent identity: component2473331
display id: component2473331
version: 1
top level: BBa_K1709006
access: public
definition: https://synbiohub.org/public/igem/BBa_J61100/1

BBa_J61100
type: SequenceAnnotation
persistent identity: annotation2473331
display id: annotation2473331
version: 1
top level: BBa_K1709006
location: https://synbiohub.org/public/igem/BBa_K1709006/annotation2473331/range2473331/1
component: BBa_J61100

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type: Range
persistent identity: range2473331
display id: range2473331
version: 1
direction: direction/reverse
top level: BBa_K1709006
start: 636
end: 647
orientation: inline

BBa_B1002
type: SequenceAnnotation
persistent identity: annotation2473341
display id: annotation2473341
version: 1
top level: BBa_K1709006
location: https://synbiohub.org/public/igem/BBa_K1709006/annotation2473341/range2473341/1
component: BBa_B1002

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persistent identity: range2473341
display id: range2473341
version: 1
direction: direction/reverse
top level: BBa_K1709006
start: 1463
end: 1496
orientation: inline

BBa_R0062
type: SequenceAnnotation
persistent identity: annotation2473343
display id: annotation2473343
version: 1
top level: BBa_K1709006
location: https://synbiohub.org/public/igem/BBa_K1709006/annotation2473343/range2473343/1
component: BBa_R0062

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type: Range
persistent identity: range2473343
display id: range2473343
version: 1
direction: direction/forward
top level: BBa_K1709006
start: 1505
end: 1559
orientation: inline

BBa_K1709004
type: SequenceAnnotation
persistent identity: annotation2473336
display id: annotation2473336
version: 1
top level: BBa_K1709006
location: https://synbiohub.org/public/igem/BBa_K1709006/annotation2473336/range2473336/1
component: BBa_K1709004

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type: Range
persistent identity: range2473336
display id: range2473336
version: 1
direction: direction/reverse
top level: BBa_K1709006
start: 654
end: 1454
orientation: inline

BBa_K1709001
type: SequenceAnnotation
persistent identity: annotation2473330
display id: annotation2473330
version: 1
top level: BBa_K1709006
location: https://synbiohub.org/public/igem/BBa_K1709006/annotation2473330/range2473330/1
component: BBa_K1709001

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type: Range
persistent identity: range2473330
display id: range2473330
version: 1
direction: direction/reverse
top level: BBa_K1709006
start: 1
end: 627
orientation: inline

rhIR
type: ComponentDefinition
persistent identity: BBa C0171
display id: BBa_C0171
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: rhlR repressor/activator from P. aeruginosa PA3477 (no LVA)
creator: jcbraff
created: 2004-05-26T11:00:00Z
modified: 2015-08-31T04:07:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_1_
m user id: 0
owner id: 61
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: same as C0071 except no LVA tag
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_C0171_sequence/1

VirA/G
type: ComponentDefinition
persistent identity: BBa K389017
display id: BBa_K389017
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: VirA/G signaling system without read-out
creator: Jonas Aretz
created: 2010-09-27T11:00:00Z
modified: 2015-05-08T01:12:19Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _517_
m user id: 0
owner id: 6670
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This BioBrick is a virA/G signaling system without read-out. It contains an unmutated ''virA'' gene and a ''virG'' gene that works without ''rpoA'' from ''A. tumefaciens'' and a ''vir'' promoter.
mutable notes: This BioBrick can be used to control any gene with a ''vir'' promoter
mutable provenance: ''A. tumefaciens'' C58 TI-plasmid, partsregistry, Mr. Gene
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
component: BBa_K389003
component: BBa_J23110
component: BBa_K389010
component: BBa_K389002
component: BBa_B0017
sequence annotation: BBa_K389010
sequence annotation: BBa_B0017
sequence annotation: BBa_K389002
sequence annotation: BBa_J23110
sequence annotation: BBa_K389003
sequence: https://synbiohub.org/public/igem/BBa_K389017_sequence/1

BBa_K389003
type: Component
persistent identity: component2226711
display id: component2226711
version: 1
top level: VirA/G
access: public
definition: https://synbiohub.org/public/igem/BBa_K389003/1

BBa_J23110
type: Component
persistent identity: component2226697
display id: component2226697
version: 1
top level: VirA/G
access: public
definition: https://synbiohub.org/public/igem/BBa_J23110/1

BBa_K389010
type: Component
persistent identity: component2226696
display id: component2226696
version: 1
top level: VirA/G
access: public
definition: https://synbiohub.org/public/igem/BBa_K389010/1

BBa_K389002
type: Component
persistent identity: component2226701
display id: component2226701
version: 1
top level: VirA/G
access: public
definition: virG

BBa_B0017
type: Component
persistent identity: component2226706
display id: component2226706
version: 1
top level: VirA/G
access: public
definition: https://synbiohub.org/public/igem/BBa_B0017/1

BBa_K389010
type: SequenceAnnotation
persistent identity: annotation2226696
display id: annotation2226696
version: 1
top level: VirA/G
location: https://synbiohub.org/public/igem/BBa_K389017/annotation2226696/range2226696/1
component: BBa_K389010

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type: Range
persistent identity: range2226696
display id: range2226696
version: 1
direction: direction/reverse
top level: VirA/G
start: 1
end: 2566
orientation: inline

BBa_B0017
type: SequenceAnnotation
persistent identity: annotation2226706
display id: annotation2226706
version: 1
top level: VirA/G
location: https://synbiohub.org/public/igem/BBa_K389017/annotation2226706/range2226706/1
component: BBa_B0017

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type: Range
persistent identity: range2226706
display id: range2226706
version: 1
direction: direction/reverse
top level: VirA/G
start: 3366
end: 3533
orientation: inline

BBa_K389002
type: SequenceAnnotation
persistent identity: annotation2226701
display id: annotation2226701
version: 1
top level: VirA/G
location: https://synbiohub.org/public/igem/BBa_K389017/annotation2226701/range2226701/1
component: BBa_K389002

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type: Range
persistent identity: range2226701
display id: range2226701
version: 1
direction: direction/reverse
top level: VirA/G
start: 2618
end: 3357
orientation: inline

BBa_J23110
type: SequenceAnnotation
persistent identity: annotation2226697
display id: annotation2226697
version: 1
top level: VirA/G
location: https://synbiohub.org/public/igem/BBa_K389017/annotation2226697/range2226697/1
component: BBa_J23110

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type: Range
persistent identity: range2226697
display id: range2226697
version: 1
direction: direction/reverse
top level: VirA/G
start: 2575
end: 2609
orientation: inline

BBa_K389003
type: SequenceAnnotation
persistent identity: annotation2226711
display id: annotation2226711
version: 1
top level: VirA/G
location: https://synbiohub.org/public/igem/BBa_K389017/annotation2226711/range2226711/1
component: BBa_K389003

https://synbiohub.org/public/igem/BBa_K389017/annotation2226711/range2226711/1
type: Range
persistent identity: range2226711
display id: range2226711
version: 1
direction: direction/reverse
top level: VirA/G
start: 3542
end: 3699
orientation: inline

BBa_K1486011
type: ComponentDefinition
persistent identity: BBa K1486011
display id: BBa_K1486011
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CxpR & Split IFP1.4 [Cterm + Nterm]
creator: iGem EPFL 2014
created: 2014-08-26T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part has two fused proteins downstream of an arabinose-inducible promoter: CpxR-IFP[1] and CpxR-IFP[2]. This construct was made in all orientations: - CpxR-IFP[1] + CpxR-IFP[2] (BBa_K1286008) - IFP[1]-CpxR + CpxR-IFP[2] (BBa_K1486010) - CpxR-IFP[1] + IFP[2]-CpxR (BBa_K1486011)
mutable notes: All orientations were made, so all dimerizations could be tested (see main page for biobrick numbers). Flexible linkers were used for each of the fused proteins, and an RBS was put before each one of them.
mutable provenance: From Michnick Article. To Edit
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0034
component: BBa_K1486004
component: BBa_K1486006
component: BBa_K1486000
component: BBa_K1486007
component: BBa_I0500
sequence annotation: BBa_I0500
sequence annotation: BBa_K1486004
sequence annotation: BBa_K1486000
sequence annotation: BBa_K1486006
sequence annotation: BBa_K1486007
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K1486011_sequence/1

BBa_B0034
type: Component
persistent identity: component2382245
display id: component2382245
version: 1
top level: BBa_K1486011
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K1486004
type: Component
persistent identity: component2382250
display id: component2382250
version: 1
top level: BBa_K1486011
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486004/1

BBa_K1486006
type: Component
persistent identity: component2382243
display id: component2382243
version: 1
top level: BBa_K1486011
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486006/1

BBa_K1486000
type: Component
persistent identity: component2382254
display id: component2382254
version: 1
top level: BBa_K1486011
access: public
definition: BBa_K1486000

BBa_K1486000
type: Component
persistent identity: component2382241
display id: component2382241
version: 1
top level: BBa_K1486011
access: public
definition: BBa_K1486000

BBa_K1486007
type: Component
persistent identity: component2382249
display id: component2382249
version: 1
top level: BBa_K1486011
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486007/1

BBa_I0500
type: Component
persistent identity: component2382235
display id: component2382235
version: 1
top level: BBa_K1486011
access: public
definition: https://synbiohub.org/public/igem/BBa_I0500/1

BBa_K1486004
type: Component
persistent identity: component2382242
display id: component2382242
version: 1
top level: BBa_K1486011
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486004/1

BBa_B0034
type: Component
persistent identity: component2382237
display id: component2382237
version: 1
top level: BBa_K1486011
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_I0500
type: SequenceAnnotation
persistent identity: annotation2382235
display id: annotation2382235
version: 1
top level: BBa_K1486011
location: https://synbiohub.org/public/igem/BBa_K1486011/annotation2382235/range2382235/1
component: BBa_I0500

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type: Range
persistent identity: range2382235
display id: range2382235
version: 1
direction: direction/reverse
top level: BBa_K1486011
start: 1
end: 1210
orientation: inline

BBa_K1486004
type: SequenceAnnotation
persistent identity: annotation2382250
display id: annotation2382250
version: 1
top level: BBa_K1486011
location: https://synbiohub.org/public/igem/BBa_K1486011/annotation2382250/range2382250/1
component: BBa_K1486004

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type: Range
persistent identity: range2382250
display id: range2382250
version: 1
direction: direction/reverse
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end: 3157
orientation: inline

BBa_K1486000
type: SequenceAnnotation
persistent identity: annotation2382241
display id: annotation2382241
version: 1
top level: BBa_K1486011
location: https://synbiohub.org/public/igem/BBa_K1486011/annotation2382241/range2382241/1
component: BBa_K1486000

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type: Range
persistent identity: range2382241
display id: range2382241
version: 1
direction: direction/reverse
top level: BBa_K1486011
start: 1223
end: 1924
orientation: inline

BBa_K1486006
type: SequenceAnnotation
persistent identity: annotation2382243
display id: annotation2382243
version: 1
top level: BBa_K1486011
location: https://synbiohub.org/public/igem/BBa_K1486011/annotation2382243/range2382243/1
component: BBa_K1486006

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type: Range
persistent identity: range2382243
display id: range2382243
version: 1
direction: direction/reverse
top level: BBa_K1486011
start: 1955
end: 2536
orientation: inline

BBa_K1486007
type: SequenceAnnotation
persistent identity: annotation2382249
display id: annotation2382249
version: 1
top level: BBa_K1486011
location: https://synbiohub.org/public/igem/BBa_K1486011/annotation2382249/range2382249/1
component: BBa_K1486007

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type: Range
persistent identity: range2382249
display id: range2382249
version: 1
direction: direction/reverse
top level: BBa_K1486011
start: 2549
end: 3127
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2382237
display id: annotation2382237
version: 1
top level: BBa_K1486011
location: https://synbiohub.org/public/igem/BBa_K1486011/annotation2382237/range2382237/1
component: BBa_B0034

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type: Range
persistent identity: range2382237
display id: range2382237
version: 1
direction: direction/reverse
top level: BBa_K1486011
start: 1211
end: 1222
orientation: inline

BBa_K1486004
type: SequenceAnnotation
persistent identity: annotation2382242
display id: annotation2382242
version: 1
top level: BBa_K1486011
location: https://synbiohub.org/public/igem/BBa_K1486011/annotation2382242/range2382242/1
component: BBa_K1486004

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type: Range
persistent identity: range2382242
display id: range2382242
version: 1
direction: direction/reverse
top level: BBa_K1486011
start: 1925
end: 1954
orientation: inline

BBa_K1486000
type: SequenceAnnotation
persistent identity: annotation2382254
display id: annotation2382254
version: 1
top level: BBa_K1486011
location: https://synbiohub.org/public/igem/BBa_K1486011/annotation2382254/range2382254/1
component: BBa_K1486000

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type: Range
persistent identity: range2382254
display id: range2382254
version: 1
direction: direction/reverse
top level: BBa_K1486011
start: 3158
end: 3859
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2382245
display id: annotation2382245
version: 1
top level: BBa_K1486011
location: https://synbiohub.org/public/igem/BBa_K1486011/annotation2382245/range2382245/1
component: BBa_B0034

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type: Range
persistent identity: range2382245
display id: range2382245
version: 1
direction: direction/reverse
top level: BBa_K1486011
start: 2537
end: 2548
orientation: inline

BBa_S04301
type: ComponentDefinition
persistent identity: BBa S04301
display id: BBa_S04301
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: C0079:B0015
creator: UNAM FCiencias-CINVESTAV IRA
created: 2009-10-11T11:00:00Z
modified: 2015-05-08T01:14:38Z
discontinued: false
dominant: false
experience: experience/None
group u list: _9_
m user id: 0
owner id: 1955
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_C0079
component: BBa_B0015
sequence annotation: BBa_C0079
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_S04301_sequence/1

BBa_C0079
type: Component
persistent identity: component2246626
display id: component2246626
version: 1
top level: BBa_S04301
access: public
definition: lasr

BBa_B0015
type: Component
persistent identity: component2246633
display id: component2246633
version: 1
top level: BBa_S04301
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_C0079
type: SequenceAnnotation
persistent identity: annotation2246626
display id: annotation2246626
version: 1
top level: BBa_S04301
location: https://synbiohub.org/public/igem/BBa_S04301/annotation2246626/range2246626/1
component: BBa_C0079

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type: Range
persistent identity: range2246626
display id: range2246626
version: 1
direction: direction/reverse
top level: BBa_S04301
start: 1
end: 781
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2246633
display id: annotation2246633
version: 1
top level: BBa_S04301
location: https://synbiohub.org/public/igem/BBa_S04301/annotation2246633/range2246633/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_S04301/annotation2246633/range2246633/1
type: Range
persistent identity: range2246633
display id: range2246633
version: 1
direction: direction/reverse
top level: BBa_S04301
start: 790
end: 918
orientation: inline

BBa_K1031211
type: ComponentDefinition
persistent identity: BBa K1031211
display id: BBa_K1031211
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Pr-DmpR
creator: He Shuaixin
created: 2013-10-20T11:00:00Z
modified: 2015-05-08T01:08:47Z
discontinued: false
dominant: false
group u list: _1338_
m user id: 0
owner id: 15843
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Pr-DmpR::pSB1C3
mutable notes: molecular cloning
mutable provenance: Pseudomonas.putida
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Pr
sequence annotation: DmpR
sequence: https://synbiohub.org/public/igem/BBa_K1031211_sequence/1

Pr
type: SequenceAnnotation
persistent identity: annotation2369617
display id: annotation2369617
version: 1
top level: BBa_K1031211
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1031211/annotation2369617/range2369617/1

https://synbiohub.org/public/igem/BBa_K1031211/annotation2369617/range2369617/1
type: Range
persistent identity: range2369617
display id: range2369617
version: 1
direction: direction/forward
top level: BBa_K1031211
start: 1
end: 74
orientation: inline

DmpR
type: SequenceAnnotation
persistent identity: annotation2369618
display id: annotation2369618
version: 1
top level: BBa_K1031211
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1031211/annotation2369618/range2369618/1

https://synbiohub.org/public/igem/BBa_K1031211/annotation2369618/range2369618/1
type: Range
persistent identity: range2369618
display id: range2369618
version: 1
direction: direction/forward
top level: BBa_K1031211
start: 75
end: 1766
orientation: inline

BBa_K108021
type: ComponentDefinition
persistent identity: BBa K108021
display id: BBa_K108021
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Fis
creator: Qi Liu
created: 2008-10-22T11:00:00Z
modified: 2015-05-08T01:09:04Z
discontinued: false
dominant: false
experience: experience/None
group u list: _222_
m user id: 0
owner id: 2671
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: global DNA-binding transcriptional dual regulator
mutable notes: PCR using primers with biobrick prefix and surfix, and then cloned into pSB1AC3.
mutable provenance: E.coli K12 DH10B genome
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Fis
sequence: https://synbiohub.org/public/igem/BBa_K108021_sequence/1

Fis
type: SequenceAnnotation
persistent identity: annotation1986976
display id: annotation1986976
version: 1
top level: BBa_K108021
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K108021/annotation1986976/range1986976/1

https://synbiohub.org/public/igem/BBa_K108021/annotation1986976/range1986976/1
type: Range
persistent identity: range1986976
display id: range1986976
version: 1
direction: direction/forward
top level: BBa_K108021
start: 1
end: 294
orientation: inline

BBa_K1031911
type: ComponentDefinition
persistent identity: BBa K1031911
display id: BBa_K1031911
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: XylS-Terminator
creator: He Shuaixin
created: 2013-08-31T11:00:00Z
modified: 2015-05-08T01:08:48Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1338_
m user id: 0
owner id: 15843
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: XylS senses benzoate and its substitutes, it is the transcriptional activation factor that activates the expression of meta-operon for benzoate degradation on TOL plasmid pWW0 in Pseudomonas putida. This part contains XylS coding sequence with terminator. To complete the sensor, please add promoter before XylS coding sequence.
mutable notes: Molecular cloning to add terminator to XylS coding sequence.
mutable provenance: XylS comes from Pseudomonas putida, sequence found through Genbank: http://www.ncbi.nlm.nih.gov/nuccore/M10143.1
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: TGA stop coden
sequence annotation: B0015
sequence annotation: XylS CDS
sequence annotation: ATG start coden
sequence: https://synbiohub.org/public/igem/BBa_K1031911_sequence/1

TGA stop coden
type: SequenceAnnotation
persistent identity: annotation2334325
display id: annotation2334325
version: 1
top level: BBa_K1031911
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1031911/annotation2334325/range2334325/1

https://synbiohub.org/public/igem/BBa_K1031911/annotation2334325/range2334325/1
type: Range
persistent identity: range2334325
display id: range2334325
version: 1
direction: direction/forward
top level: BBa_K1031911
start: 964
end: 966
orientation: inline

B0015
type: SequenceAnnotation
persistent identity: annotation2334324
display id: annotation2334324
version: 1
top level: BBa_K1031911
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1031911/annotation2334324/range2334324/1

https://synbiohub.org/public/igem/BBa_K1031911/annotation2334324/range2334324/1
type: Range
persistent identity: range2334324
display id: range2334324
version: 1
direction: direction/forward
top level: BBa_K1031911
start: 974
end: 1103
orientation: inline

XylS CDS
type: SequenceAnnotation
persistent identity: annotation2334323
display id: annotation2334323
version: 1
top level: BBa_K1031911
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1031911/annotation2334323/range2334323/1

https://synbiohub.org/public/igem/BBa_K1031911/annotation2334323/range2334323/1
type: Range
persistent identity: range2334323
display id: range2334323
version: 1
direction: direction/forward
top level: BBa_K1031911
start: 1
end: 966
orientation: inline

ATG start coden
type: SequenceAnnotation
persistent identity: annotation2334322
display id: annotation2334322
version: 1
top level: BBa_K1031911
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1031911/annotation2334322/range2334322/1

https://synbiohub.org/public/igem/BBa_K1031911/annotation2334322/range2334322/1
type: Range
persistent identity: range2334322
display id: range2334322
version: 1
direction: direction/forward
top level: BBa_K1031911
start: 1
end: 3
orientation: inline

BBa_K1067008
type: ComponentDefinition
persistent identity: BBa K1067008
display id: BBa_K1067008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: mCherry downstream tight pBAD
creator: Kristian Davidsen
created: 2013-09-27T11:00:00Z
modified: 2015-05-08T01:09:00Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1376_
m user id: 0
owner id: 16028
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: ggg
mutable notes: ggg
mutable provenance: ggg
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Measurement
sequence annotation: mCherry
sequence annotation: araO2
sequence annotation: araI2
sequence annotation: araO1R
sequence annotation: araO1L
sequence annotation: araI1
sequence annotation: -10
sequence annotation: -35
sequence annotation: Tight pBAD promoter
sequence annotation: rbs
sequence annotation: araC
sequence annotation: CRP binding site
sequence annotation: BBa_K1067007
sequence: https://synbiohub.org/public/igem/BBa_K1067008_sequence/1

mCherry
type: SequenceAnnotation
persistent identity: annotation2366501
display id: annotation2366501
version: 1
top level: BBa_K1067008
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1067008/annotation2366501/range2366501/1

https://synbiohub.org/public/igem/BBa_K1067008/annotation2366501/range2366501/1
type: Range
persistent identity: range2366501
display id: range2366501
version: 1
direction: direction/forward
top level: BBa_K1067008
start: 1237
end: 1947
orientation: inline

araO2
type: SequenceAnnotation
persistent identity: annotation2366491
display id: annotation2366491
version: 1
top level: BBa_K1067008
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1067008/annotation2366491/range2366491/1

https://synbiohub.org/public/igem/BBa_K1067008/annotation2366491/range2366491/1
type: Range
persistent identity: range2366491
display id: range2366491
version: 1
direction: direction/forward
top level: BBa_K1067008
start: 908
end: 924
orientation: inline

araI2
type: SequenceAnnotation
persistent identity: annotation2366496
display id: annotation2366496
version: 1
top level: BBa_K1067008
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1067008/annotation2366496/range2366496/1

https://synbiohub.org/public/igem/BBa_K1067008/annotation2366496/range2366496/1
type: Range
persistent identity: range2366496
display id: range2366496
version: 1
direction: direction/forward
top level: BBa_K1067008
start: 1140
end: 1157
orientation: inline

araO1R
type: SequenceAnnotation
persistent identity: annotation2366493
display id: annotation2366493
version: 1
top level: BBa_K1067008
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1067008/annotation2366493/range2366493/1

https://synbiohub.org/public/igem/BBa_K1067008/annotation2366493/range2366493/1
type: Range
persistent identity: range2366493
display id: range2366493
version: 1
direction: direction/forward
top level: BBa_K1067008
start: 1066
end: 1082
orientation: inline

araO1L
type: SequenceAnnotation
persistent identity: annotation2366492
display id: annotation2366492
version: 1
top level: BBa_K1067008
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1067008/annotation2366492/range2366492/1

https://synbiohub.org/public/igem/BBa_K1067008/annotation2366492/range2366492/1
type: Range
persistent identity: range2366492
display id: range2366492
version: 1
direction: direction/forward
top level: BBa_K1067008
start: 1045
end: 1061
orientation: inline

araI1
type: SequenceAnnotation
persistent identity: annotation2366495
display id: annotation2366495
version: 1
top level: BBa_K1067008
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1067008/annotation2366495/range2366495/1

https://synbiohub.org/public/igem/BBa_K1067008/annotation2366495/range2366495/1
type: Range
persistent identity: range2366495
display id: range2366495
version: 1
direction: direction/forward
top level: BBa_K1067008
start: 1119
end: 1135
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2366499
display id: annotation2366499
version: 1
top level: BBa_K1067008
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1067008/annotation2366499/range2366499/1

https://synbiohub.org/public/igem/BBa_K1067008/annotation2366499/range2366499/1
type: Range
persistent identity: range2366499
display id: range2366499
version: 1
direction: direction/forward
top level: BBa_K1067008
start: 1177
end: 1182
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2366498
display id: annotation2366498
version: 1
top level: BBa_K1067008
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1067008/annotation2366498/range2366498/1

https://synbiohub.org/public/igem/BBa_K1067008/annotation2366498/range2366498/1
type: Range
persistent identity: range2366498
display id: range2366498
version: 1
direction: direction/forward
top level: BBa_K1067008
start: 1153
end: 1158
orientation: inline

Tight pBAD promoter
type: SequenceAnnotation
persistent identity: annotation2366497
display id: annotation2366497
version: 1
top level: BBa_K1067008
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1067008/annotation2366497/range2366497/1

https://synbiohub.org/public/igem/BBa_K1067008/annotation2366497/range2366497/1
type: Range
persistent identity: range2366497
display id: range2366497
version: 1
direction: direction/forward
top level: BBa_K1067008
start: 1153
end: 1189
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation2366502
display id: annotation2366502
version: 1
top level: BBa_K1067008
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1067008/annotation2366502/range2366502/1

https://synbiohub.org/public/igem/BBa_K1067008/annotation2366502/range2366502/1
type: Range
persistent identity: range2366502
display id: range2366502
version: 1
direction: direction/forward
top level: BBa_K1067008
start: 1225
end: 1229
orientation: inline

araC
type: SequenceAnnotation
persistent identity: annotation2366490
display id: annotation2366490
version: 1
top level: BBa_K1067008
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1067008/annotation2366490/range2366490/1

https://synbiohub.org/public/igem/BBa_K1067008/annotation2366490/range2366490/1
type: Range
persistent identity: range2366490
display id: range2366490
version: 1
direction: direction/forward
top level: BBa_K1067008
start: 1
end: 880
orientation: inline

CRP binding site
type: SequenceAnnotation
persistent identity: annotation2366494
display id: annotation2366494
version: 1
top level: BBa_K1067008
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1067008/annotation2366494/range2366494/1

https://synbiohub.org/public/igem/BBa_K1067008/annotation2366494/range2366494/1
type: Range
persistent identity: range2366494
display id: range2366494
version: 1
direction: direction/forward
top level: BBa_K1067008
start: 1087
end: 1108
orientation: inline

BBa_K1067007
type: SequenceAnnotation
persistent identity: annotation2366500
display id: annotation2366500
version: 1
top level: BBa_K1067008
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1067008/annotation2366500/range2366500/1

https://synbiohub.org/public/igem/BBa_K1067008/annotation2366500/range2366500/1
type: Range
persistent identity: range2366500
display id: range2366500
version: 1
direction: direction/reverse
top level: BBa_K1067008
start: 1
end: 1209
orientation: inline

BBa_K1520003
type: ComponentDefinition
persistent identity: BBa K1520003
display id: BBa_K1520003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Pcons2-rbs-luxR-Ter
creator: yue wu
created: 2014-09-21T11:00:00Z
modified: 2015-05-08T01:10:48Z
discontinued: false
dominant: false
group u list: _1902_
m user id: 0
owner id: 21318
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Pcons2-rbs-luxR-Ter. It achieves a constant expression of luxR, the AHL combining protein Pcons2 is BBa_J23100
mutable notes: NONE
mutable provenance: Pcons2, luxR, Ter
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K1520029
component: BBa_J23100
component: BBa_K1520501
sequence annotation: BBa_J23100
sequence annotation: BBa_K1520501
sequence annotation: BBa_K1520029
sequence: https://synbiohub.org/public/igem/BBa_K1520003_sequence/1

BBa_K1520029
type: Component
persistent identity: component2384888
display id: component2384888
version: 1
top level: BBa_K1520003
access: public
definition: https://synbiohub.org/public/igem/BBa_K1520029/1

BBa_J23100
type: Component
persistent identity: component2384884
display id: component2384884
version: 1
top level: BBa_K1520003
access: public
definition: https://synbiohub.org/public/igem/BBa_J23100/1

BBa_K1520501
type: Component
persistent identity: component2384887
display id: component2384887
version: 1
top level: BBa_K1520003
access: public
definition: BBa_K1520501

BBa_J23100
type: SequenceAnnotation
persistent identity: annotation2384884
display id: annotation2384884
version: 1
top level: BBa_K1520003
location: https://synbiohub.org/public/igem/BBa_K1520003/annotation2384884/range2384884/1
component: BBa_J23100

https://synbiohub.org/public/igem/BBa_K1520003/annotation2384884/range2384884/1
type: Range
persistent identity: range2384884
display id: range2384884
version: 1
direction: direction/reverse
top level: BBa_K1520003
start: 1
end: 35
orientation: inline

BBa_K1520501
type: SequenceAnnotation
persistent identity: annotation2384887
display id: annotation2384887
version: 1
top level: BBa_K1520003
location: https://synbiohub.org/public/igem/BBa_K1520003/annotation2384887/range2384887/1
component: BBa_K1520501

https://synbiohub.org/public/igem/BBa_K1520003/annotation2384887/range2384887/1
type: Range
persistent identity: range2384887
display id: range2384887
version: 1
direction: direction/reverse
top level: BBa_K1520003
start: 44
end: 842
orientation: inline

BBa_K1520029
type: SequenceAnnotation
persistent identity: annotation2384888
display id: annotation2384888
version: 1
top level: BBa_K1520003
location: https://synbiohub.org/public/igem/BBa_K1520003/annotation2384888/range2384888/1
component: BBa_K1520029

https://synbiohub.org/public/igem/BBa_K1520003/annotation2384888/range2384888/1
type: Range
persistent identity: range2384888
display id: range2384888
version: 1
direction: direction/reverse
top level: BBa_K1520003
start: 851
end: 979
orientation: inline

BBa_K1709004
type: ComponentDefinition
persistent identity: BBa K1709004
display id: BBa_K1709004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LuxR-E
creator: Astrid Deryckere
created: 2015-09-17T11:00:00Z
modified: 2015-09-18T11:21:02Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2129_
m user id: 25389
owner id: 25389
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Encodes a transcription activator for the right handed Lux promotor. Two molecules of acyl-homoserine lactones (AHL) form a complex with two molecules of the LuxR protein. This complex then binds to a palyndromic site on the lux promoter, increasing its transcription rate. LuxI, LuxR and the right lux promoter are all derived from V. fischeri.
mutable notes: Biobrick BBa_C0061 still contained a Barcode sequence which was deleted. For our own use, 9 bp were exchanged to create unique restriction sites and to optimize DNA folding.
mutable provenance: Sequence is originating from V. fischeri. Here, the sequence from BBa_I0462 was used and optimized.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: LuxR
sequence annotation: ATG Start Codon
sequence annotation: E-tag
sequence annotation: Ochre Stop Codon
sequence: https://synbiohub.org/public/igem/BBa_K1709004_sequence/1

LuxR
type: SequenceAnnotation
persistent identity: annotation2470868
display id: annotation2470868
version: 1
top level: BBa_K1709004
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1709004/annotation2470868/range2470868/1

https://synbiohub.org/public/igem/BBa_K1709004/annotation2470868/range2470868/1
type: Range
persistent identity: range2470868
display id: range2470868
version: 1
direction: direction/forward
top level: BBa_K1709004
start: 1
end: 750
orientation: inline

ATG Start Codon
type: SequenceAnnotation
persistent identity: annotation2470847
display id: annotation2470847
version: 1
top level: BBa_K1709004
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1709004/annotation2470847/range2470847/1

https://synbiohub.org/public/igem/BBa_K1709004/annotation2470847/range2470847/1
type: Range
persistent identity: range2470847
display id: range2470847
version: 1
direction: direction/forward
top level: BBa_K1709004
start: 1
end: 3
orientation: inline

E-tag
type: SequenceAnnotation
persistent identity: annotation2470866
display id: annotation2470866
version: 1
top level: BBa_K1709004
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1709004/annotation2470866/range2470866/1

https://synbiohub.org/public/igem/BBa_K1709004/annotation2470866/range2470866/1
type: Range
persistent identity: range2470866
display id: range2470866
version: 1
direction: direction/forward
top level: BBa_K1709004
start: 753
end: 801
orientation: inline

Ochre Stop Codon
type: SequenceAnnotation
persistent identity: annotation2470849
display id: annotation2470849
version: 1
top level: BBa_K1709004
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1709004/annotation2470849/range2470849/1

https://synbiohub.org/public/igem/BBa_K1709004/annotation2470849/range2470849/1
type: Range
persistent identity: range2470849
display id: range2470849
version: 1
direction: direction/forward
top level: BBa_K1709004
start: 799
end: 801
orientation: inline

BBa_K389413
type: ComponentDefinition
persistent identity: BBa K389413
display id: BBa_K389413
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: vir promoter + sensitivity tuner + luciferase
creator: Jonas Aretz
created: 2010-10-10T11:00:00Z
modified: 2015-05-08T01:12:19Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _517_
m user id: 0
owner id: 6670
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part combines a mutated virG response regulator from the VirA/G receptor system under the control of a constitutive promoter with a sensitivity tuner before a luciferase gene under the control of a vir promoter. The sensitivity tuner enhances a signal from a promoter so it enhances the readout luciferase.
mutable notes: sensitivity tuner to enhance the luciferase signal
mutable provenance: * luciferase from pGL4.10luc2 (Promega) * sensitivity tuner part, terminators and constitutive promoter from the partsregistry * ''virG'' was synthesized by Mr. Gene * ''vir'' promoter from Agrobacterium tumefaciens C58, TI-plasmid
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_J23110
component: BBa_K389403
component: BBa_B0017
component: BBa_K389002
component: BBa_K389003
sequence annotation: BBa_J23110
sequence annotation: BBa_K389403
sequence annotation: BBa_K389002
sequence annotation: BBa_B0017
sequence annotation: BBa_K389003
sequence: https://synbiohub.org/public/igem/BBa_K389413_sequence/1

BBa_J23110
type: Component
persistent identity: component2300800
display id: component2300800
version: 1
top level: BBa_K389413
access: public
definition: https://synbiohub.org/public/igem/BBa_J23110/1

BBa_K389403
type: Component
persistent identity: component2300839
display id: component2300839
version: 1
top level: BBa_K389413
access: public
definition: https://synbiohub.org/public/igem/BBa_K389403/1

BBa_B0017
type: Component
persistent identity: component2300809
display id: component2300809
version: 1
top level: BBa_K389413
access: public
definition: https://synbiohub.org/public/igem/BBa_B0017/1

BBa_K389002
type: Component
persistent identity: component2300804
display id: component2300804
version: 1
top level: BBa_K389413
access: public
definition: virG

BBa_K389003
type: Component
persistent identity: component2300814
display id: component2300814
version: 1
top level: BBa_K389413
access: public
definition: https://synbiohub.org/public/igem/BBa_K389003/1

BBa_J23110
type: SequenceAnnotation
persistent identity: annotation2300800
display id: annotation2300800
version: 1
top level: BBa_K389413
location: https://synbiohub.org/public/igem/BBa_K389413/annotation2300800/range2300800/1
component: BBa_J23110

https://synbiohub.org/public/igem/BBa_K389413/annotation2300800/range2300800/1
type: Range
persistent identity: range2300800
display id: range2300800
version: 1
direction: direction/reverse
top level: BBa_K389413
start: 1
end: 35
orientation: inline

BBa_K389403
type: SequenceAnnotation
persistent identity: annotation2300839
display id: annotation2300839
version: 1
top level: BBa_K389413
location: https://synbiohub.org/public/igem/BBa_K389413/annotation2300839/range2300839/1
component: BBa_K389403

https://synbiohub.org/public/igem/BBa_K389413/annotation2300839/range2300839/1
type: Range
persistent identity: range2300839
display id: range2300839
version: 1
direction: direction/reverse
top level: BBa_K389413
start: 1134
end: 4047
orientation: inline

BBa_K389002
type: SequenceAnnotation
persistent identity: annotation2300804
display id: annotation2300804
version: 1
top level: BBa_K389413
location: https://synbiohub.org/public/igem/BBa_K389413/annotation2300804/range2300804/1
component: BBa_K389002

https://synbiohub.org/public/igem/BBa_K389413/annotation2300804/range2300804/1
type: Range
persistent identity: range2300804
display id: range2300804
version: 1
direction: direction/reverse
top level: BBa_K389413
start: 44
end: 783
orientation: inline

BBa_B0017
type: SequenceAnnotation
persistent identity: annotation2300809
display id: annotation2300809
version: 1
top level: BBa_K389413
location: https://synbiohub.org/public/igem/BBa_K389413/annotation2300809/range2300809/1
component: BBa_B0017

https://synbiohub.org/public/igem/BBa_K389413/annotation2300809/range2300809/1
type: Range
persistent identity: range2300809
display id: range2300809
version: 1
direction: direction/reverse
top level: BBa_K389413
start: 792
end: 959
orientation: inline

BBa_K389003
type: SequenceAnnotation
persistent identity: annotation2300814
display id: annotation2300814
version: 1
top level: BBa_K389413
location: https://synbiohub.org/public/igem/BBa_K389413/annotation2300814/range2300814/1
component: BBa_K389003

https://synbiohub.org/public/igem/BBa_K389413/annotation2300814/range2300814/1
type: Range
persistent identity: range2300814
display id: range2300814
version: 1
direction: direction/reverse
top level: BBa_K389413
start: 968
end: 1125
orientation: inline

BBa_K1031916
type: ComponentDefinition
persistent identity: BBa K1031916
display id: BBa_K1031916
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: J23114-XylS-Terminator
creator: He Shuaixin
created: 2013-09-08T11:00:00Z
modified: 2015-05-08T01:08:48Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1338_
m user id: 0
owner id: 15843
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: 114-XylS-TT
mutable notes: molecular cloning
mutable provenance: synthesis PSEUDOMONAS
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: J23114
sequence annotation: XylS
sequence annotation: B0015
sequence: https://synbiohub.org/public/igem/BBa_K1031916_sequence/1

J23114
type: SequenceAnnotation
persistent identity: annotation2360184
display id: annotation2360184
version: 1
top level: BBa_K1031916
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1031916/annotation2360184/range2360184/1

https://synbiohub.org/public/igem/BBa_K1031916/annotation2360184/range2360184/1
type: Range
persistent identity: range2360184
display id: range2360184
version: 1
direction: direction/forward
top level: BBa_K1031916
start: 1
end: 35
orientation: inline

XylS
type: SequenceAnnotation
persistent identity: annotation2360185
display id: annotation2360185
version: 1
top level: BBa_K1031916
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1031916/annotation2360185/range2360185/1

https://synbiohub.org/public/igem/BBa_K1031916/annotation2360185/range2360185/1
type: Range
persistent identity: range2360185
display id: range2360185
version: 1
direction: direction/forward
top level: BBa_K1031916
start: 62
end: 1027
orientation: inline

B0015
type: SequenceAnnotation
persistent identity: annotation2360187
display id: annotation2360187
version: 1
top level: BBa_K1031916
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1031916/annotation2360187/range2360187/1

https://synbiohub.org/public/igem/BBa_K1031916/annotation2360187/range2360187/1
type: Range
persistent identity: range2360187
display id: range2360187
version: 1
direction: direction/forward
top level: BBa_K1031916
start: 1034
end: 1162
orientation: inline

VP64
type: ComponentDefinition
persistent identity: BBa K1982012
display id: BBa_K1982012
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: VP64 transcription activitor
creator: Zexu Li
created: 2016-10-17T11:00:00Z
modified: 2016-10-18T09:58:31Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2449_
m user id: 30176
owner id: 30176
owning group id: 9
status: status/Planning
bookmark: false
mutable description: {| style="color:black" cellpadding="6" cellspacing="1" border="2" align="right" ! colspan="2" style="background:#FFBF00;"|VP64 |- |'''Function''' |transcription activator |- |'''RFC standard''' |[http://parts.igem.org/Help:Assembly_standard_10 RFC 10] |- |'''Backbone''' |pSB1C3 |- |'''Organism''' |''synthetic construct'' |- |'''Source''' |synthetic construct |- |'''Submitted by''' |[http://2016.igem.org/Team:NEU-China NEU-China 2016] |} Tetrameric VP16 transcription activator domain
mutable notes: synthetic construct
mutable provenance: synthetic construct
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: SV40 NLS
sequence annotation: VP16
sequence annotation: FLAG
sequence annotation: HA
sequence annotation: Linker
sequence annotation: TAATAA
sequence: https://synbiohub.org/public/igem/BBa_K1982012_sequence/1

SV40 NLS
type: SequenceAnnotation
persistent identity: annotation2517149
display id: annotation2517149
version: 1
top level: VP64
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1982012/annotation2517149/range2517149/1

https://synbiohub.org/public/igem/BBa_K1982012/annotation2517149/range2517149/1
type: Range
persistent identity: range2517149
display id: range2517149
version: 1
direction: direction/forward
top level: VP64
start: 1
end: 21
orientation: inline

VP16
type: SequenceAnnotation
persistent identity: annotation2517150
display id: annotation2517150
version: 1
top level: VP64
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1982012/annotation2517150/range2517150/1

https://synbiohub.org/public/igem/BBa_K1982012/annotation2517150/range2517150/1
type: Range
persistent identity: range2517150
display id: range2517150
version: 1
direction: direction/forward
top level: VP64
start: 22
end: 63
orientation: inline

FLAG
type: SequenceAnnotation
persistent identity: annotation2517156
display id: annotation2517156
version: 1
top level: VP64
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1982012/annotation2517156/range2517156/1

https://synbiohub.org/public/igem/BBa_K1982012/annotation2517156/range2517156/1
type: Range
persistent identity: range2517156
display id: range2517156
version: 1
direction: direction/forward
top level: VP64
start: 217
end: 240
orientation: inline

HA
type: SequenceAnnotation
persistent identity: annotation2517155
display id: annotation2517155
version: 1
top level: VP64
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1982012/annotation2517155/range2517155/1

https://synbiohub.org/public/igem/BBa_K1982012/annotation2517155/range2517155/1
type: Range
persistent identity: range2517155
display id: range2517155
version: 1
direction: direction/forward
top level: VP64
start: 187
end: 213
orientation: inline

VP16
type: SequenceAnnotation
persistent identity: annotation2517153
display id: annotation2517153
version: 1
top level: VP64
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1982012/annotation2517153/range2517153/1

https://synbiohub.org/public/igem/BBa_K1982012/annotation2517153/range2517153/1
type: Range
persistent identity: range2517153
display id: range2517153
version: 1
direction: direction/forward
top level: VP64
start: 142
end: 180
orientation: inline

VP16
type: SequenceAnnotation
persistent identity: annotation2517151
display id: annotation2517151
version: 1
top level: VP64
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1982012/annotation2517151/range2517151/1

https://synbiohub.org/public/igem/BBa_K1982012/annotation2517151/range2517151/1
type: Range
persistent identity: range2517151
display id: range2517151
version: 1
direction: direction/forward
top level: VP64
start: 64
end: 102
orientation: inline

VP16
type: SequenceAnnotation
persistent identity: annotation2517152
display id: annotation2517152
version: 1
top level: VP64
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1982012/annotation2517152/range2517152/1

https://synbiohub.org/public/igem/BBa_K1982012/annotation2517152/range2517152/1
type: Range
persistent identity: range2517152
display id: range2517152
version: 1
direction: direction/forward
top level: VP64
start: 103
end: 141
orientation: inline

Linker
type: SequenceAnnotation
persistent identity: annotation2517154
display id: annotation2517154
version: 1
top level: VP64
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1982012/annotation2517154/range2517154/1

https://synbiohub.org/public/igem/BBa_K1982012/annotation2517154/range2517154/1
type: Range
persistent identity: range2517154
display id: range2517154
version: 1
direction: direction/forward
top level: VP64
start: 181
end: 186
orientation: inline

TAATAA
type: SequenceAnnotation
persistent identity: annotation2517157
display id: annotation2517157
version: 1
top level: VP64
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1982012/annotation2517157/range2517157/1

https://synbiohub.org/public/igem/BBa_K1982012/annotation2517157/range2517157/1
type: Range
persistent identity: range2517157
display id: range2517157
version: 1
direction: direction/forward
top level: VP64
start: 241
end: 246
orientation: inline

BBa_J07009
type: ComponentDefinition
persistent identity: BBa J07009
display id: BBa_J07009
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: toxicity-gene activator from Vibrio cholerae
creator: mit_igem_2005
created: 2005-07-06T11:00:00Z
modified: 2015-08-31T04:08:19Z
discontinued: false
dominant: false
experience: experience/None
group u list: _21_
m user id: 0
owner id: 363
owning group id: 21
sample status: In stock
status: status/Available
bookmark: true
mutable description: shortened BBa_J07008. Typical ToxR-periplasm-region subsitutions are done @ aa 211. explanation of ToxR domains @ http://model.mit.edu/igem/images/d/d7/Kolmar95.pdf ELIMINATE XbaI SITE PLZZZZZ
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Start Codon
sequence annotation: Cytoplasmic Region
sequence annotation: Periplasmic Region
sequence annotation: Coding Sequence for ToxR'
sequence annotation: Transmembrane Region
sequence: https://synbiohub.org/public/igem/BBa_J07009_sequence/1

Start Codon
type: SequenceAnnotation
persistent identity: annotation1668508
display id: annotation1668508
version: 1
top level: BBa_J07009
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_J07009/annotation1668508/range1668508/1

https://synbiohub.org/public/igem/BBa_J07009/annotation1668508/range1668508/1
type: Range
persistent identity: range1668508
display id: range1668508
version: 1
direction: direction/forward
top level: BBa_J07009
start: 6
end: 8
orientation: inline

Cytoplasmic Region
type: SequenceAnnotation
persistent identity: annotation1668506
display id: annotation1668506
version: 1
top level: BBa_J07009
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J07009/annotation1668506/range1668506/1

https://synbiohub.org/public/igem/BBa_J07009/annotation1668506/range1668506/1
type: Range
persistent identity: range1668506
display id: range1668506
version: 1
direction: direction/forward
top level: BBa_J07009
start: 1
end: 546
orientation: inline

Periplasmic Region
type: SequenceAnnotation
persistent identity: annotation1668510
display id: annotation1668510
version: 1
top level: BBa_J07009
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J07009/annotation1668510/range1668510/1

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type: Range
persistent identity: range1668510
display id: range1668510
version: 1
direction: direction/forward
top level: BBa_J07009
start: 595
end: 630
orientation: inline

Coding Sequence for ToxR'
type: SequenceAnnotation
persistent identity: annotation1668507
display id: annotation1668507
version: 1
top level: BBa_J07009
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J07009/annotation1668507/range1668507/1

https://synbiohub.org/public/igem/BBa_J07009/annotation1668507/range1668507/1
type: Range
persistent identity: range1668507
display id: range1668507
version: 1
direction: direction/forward
top level: BBa_J07009
start: 1
end: 630
orientation: inline

Transmembrane Region
type: SequenceAnnotation
persistent identity: annotation1668509
display id: annotation1668509
version: 1
top level: BBa_J07009
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J07009/annotation1668509/range1668509/1

https://synbiohub.org/public/igem/BBa_J07009/annotation1668509/range1668509/1
type: Range
persistent identity: range1668509
display id: range1668509
version: 1
direction: direction/forward
top level: BBa_J07009
start: 547
end: 594
orientation: inline

BBa_K1486008
type: ComponentDefinition
persistent identity: BBa K1486008
display id: BBa_K1486008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CxpR & Split IFP1.4 [Cterm + Cterm][1]
creator: iGem EPFL 2014
created: 2014-08-25T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part has two fused proteins downstream of an arabinose-inducible promoter: CpxR-IFP[1] and CpxR-IFP[2]. This construct was made in all orientations: - IFP[1]-CpxR + IFP[2]-CpxR (BBa_K1286009) - IFP[1]-CpxR + CpxR-IFP[2] (BBa_K1486010) - CpxR-IFP[1] + IFP[2]-CpxR (BBa_K1486011)
mutable notes: All orientations were made, so all dimerizations could be tested (see main page for biobrick numbers). Flexible linkers were used for each of the fused proteins, and an RBS was put before each one of them.
mutable provenance: From Michnick Article. To Edit
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K1486004
component: BBa_I0500
component: BBa_K1486052
component: BBa_K1486006
component: BBa_K1486007
component: BBa_B0034
component: BBa_K1486000
sequence annotation: BBa_K1486000
sequence annotation: BBa_I0500
sequence annotation: BBa_B0034
sequence annotation: BBa_K1486004
sequence annotation: BBa_K1486006
sequence annotation: BBa_K1486007
sequence annotation: BBa_K1486052
sequence: https://synbiohub.org/public/igem/BBa_K1486008_sequence/1

BBa_K1486004
type: Component
persistent identity: component2418041
display id: component2418041
version: 1
top level: BBa_K1486008
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486004/1

BBa_I0500
type: Component
persistent identity: component2418034
display id: component2418034
version: 1
top level: BBa_K1486008
access: public
definition: https://synbiohub.org/public/igem/BBa_I0500/1

BBa_K1486052
type: Component
persistent identity: component2418046
display id: component2418046
version: 1
top level: BBa_K1486008
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486052/1

BBa_K1486006
type: Component
persistent identity: component2418045
display id: component2418045
version: 1
top level: BBa_K1486008
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486006/1

BBa_K1486007
type: Component
persistent identity: component2418057
display id: component2418057
version: 1
top level: BBa_K1486008
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486007/1

BBa_K1486004
type: Component
persistent identity: component2418053
display id: component2418053
version: 1
top level: BBa_K1486008
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486004/1

BBa_B0034
type: Component
persistent identity: component2418036
display id: component2418036
version: 1
top level: BBa_K1486008
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K1486000
type: Component
persistent identity: component2418040
display id: component2418040
version: 1
top level: BBa_K1486008
access: public
definition: BBa_K1486000

BBa_B0034
type: Component
persistent identity: component2418048
display id: component2418048
version: 1
top level: BBa_K1486008
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K1486000
type: Component
persistent identity: component2418052
display id: component2418052
version: 1
top level: BBa_K1486008
access: public
definition: BBa_K1486000

BBa_K1486000
type: SequenceAnnotation
persistent identity: annotation2418040
display id: annotation2418040
version: 1
top level: BBa_K1486008
location: https://synbiohub.org/public/igem/BBa_K1486008/annotation2418040/range2418040/1
component: BBa_K1486000

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type: Range
persistent identity: range2418040
display id: range2418040
version: 1
direction: direction/reverse
top level: BBa_K1486008
start: 1223
end: 1924
orientation: inline

BBa_I0500
type: SequenceAnnotation
persistent identity: annotation2418034
display id: annotation2418034
version: 1
top level: BBa_K1486008
location: https://synbiohub.org/public/igem/BBa_K1486008/annotation2418034/range2418034/1
component: BBa_I0500

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type: Range
persistent identity: range2418034
display id: range2418034
version: 1
direction: direction/reverse
top level: BBa_K1486008
start: 1
end: 1210
orientation: inline

BBa_K1486000
type: SequenceAnnotation
persistent identity: annotation2418052
display id: annotation2418052
version: 1
top level: BBa_K1486008
location: https://synbiohub.org/public/igem/BBa_K1486008/annotation2418052/range2418052/1
component: BBa_K1486000

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type: Range
persistent identity: range2418052
display id: range2418052
version: 1
direction: direction/reverse
top level: BBa_K1486008
start: 2410
end: 3111
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2418036
display id: annotation2418036
version: 1
top level: BBa_K1486008
location: https://synbiohub.org/public/igem/BBa_K1486008/annotation2418036/range2418036/1
component: BBa_B0034

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type: Range
persistent identity: range2418036
display id: range2418036
version: 1
direction: direction/reverse
top level: BBa_K1486008
start: 1211
end: 1222
orientation: inline

BBa_K1486004
type: SequenceAnnotation
persistent identity: annotation2418053
display id: annotation2418053
version: 1
top level: BBa_K1486008
location: https://synbiohub.org/public/igem/BBa_K1486008/annotation2418053/range2418053/1
component: BBa_K1486004

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type: Range
persistent identity: range2418053
display id: range2418053
version: 1
direction: direction/reverse
top level: BBa_K1486008
start: 3112
end: 3141
orientation: inline

BBa_K1486006
type: SequenceAnnotation
persistent identity: annotation2418045
display id: annotation2418045
version: 1
top level: BBa_K1486008
location: https://synbiohub.org/public/igem/BBa_K1486008/annotation2418045/range2418045/1
component: BBa_K1486006

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type: Range
persistent identity: range2418045
display id: range2418045
version: 1
direction: direction/reverse
top level: BBa_K1486008
start: 1955
end: 2359
orientation: inline

BBa_K1486007
type: SequenceAnnotation
persistent identity: annotation2418057
display id: annotation2418057
version: 1
top level: BBa_K1486008
location: https://synbiohub.org/public/igem/BBa_K1486008/annotation2418057/range2418057/1
component: BBa_K1486007

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type: Range
persistent identity: range2418057
display id: range2418057
version: 1
direction: direction/reverse
top level: BBa_K1486008
start: 3142
end: 3726
orientation: inline

BBa_K1486052
type: SequenceAnnotation
persistent identity: annotation2418046
display id: annotation2418046
version: 1
top level: BBa_K1486008
location: https://synbiohub.org/public/igem/BBa_K1486008/annotation2418046/range2418046/1
component: BBa_K1486052

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type: Range
persistent identity: range2418046
display id: range2418046
version: 1
direction: direction/reverse
top level: BBa_K1486008
start: 2360
end: 2397
orientation: inline

BBa_K1486004
type: SequenceAnnotation
persistent identity: annotation2418041
display id: annotation2418041
version: 1
top level: BBa_K1486008
location: https://synbiohub.org/public/igem/BBa_K1486008/annotation2418041/range2418041/1
component: BBa_K1486004

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type: Range
persistent identity: range2418041
display id: range2418041
version: 1
direction: direction/reverse
top level: BBa_K1486008
start: 1925
end: 1954
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2418048
display id: annotation2418048
version: 1
top level: BBa_K1486008
location: https://synbiohub.org/public/igem/BBa_K1486008/annotation2418048/range2418048/1
component: BBa_B0034

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type: Range
persistent identity: range2418048
display id: range2418048
version: 1
direction: direction/reverse
top level: BBa_K1486008
start: 2398
end: 2409
orientation: inline

BBa_K1946000
type: ComponentDefinition
persistent identity: BBa K1946000
display id: BBa_K1946000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: ChnR
creator: Musa Efe Işılak
created: 2016-10-12T11:00:00Z
modified: 2016-10-13T04:34:20Z
discontinued: false
dominant: false
group u list: _2413_
m user id: 29843
owner id: 29843
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This part codes for ChnR protein which is a Xyls/AraC-type transcriptional activator from Acinetobacter sp. It responds to the presence of cyclohexanone by inducing expression of ChnB protein. Iwaki H, Hasegawa Y, Teraoka M, Tokuyama T, Bergeron H, Lau PC. Identification of a transcriptional activator (ChnR) and a 6-oxohexanoate dehydrogenase (ChnE) in the cyclohexanol catabolic pathway in Acinetobacter sp. Strain NCIMB 9871 and localization of the genes that encode them. Appl Environ Microbiol. 1999;65(11):5158-62. Steigedal, Magnus and Svein Valla. "The Acinetobacter Sp. Chnb Promoter Together With Its Cognate Positive Regulator Chnr Is An Attractive New Candidate For Metabolic Engineering Applications In Bacteria". Metabolic Engineering 10.2 (2008): 121-129. Web.
mutable notes: Codon optimized for E. Coli k12
mutable provenance: The source of this part is amino acid sequence from GenBank: BAA86295.1
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: start codon
sequence annotation: stop codon
sequence annotation: ChnR coding region
sequence: https://synbiohub.org/public/igem/BBa_K1946000_sequence/1

start codon
type: SequenceAnnotation
persistent identity: annotation2497187
display id: annotation2497187
version: 1
top level: BBa_K1946000
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1946000/annotation2497187/range2497187/1

https://synbiohub.org/public/igem/BBa_K1946000/annotation2497187/range2497187/1
type: Range
persistent identity: range2497187
display id: range2497187
version: 1
direction: direction/forward
top level: BBa_K1946000
start: 1
end: 3
orientation: inline

stop codon
type: SequenceAnnotation
persistent identity: annotation2497192
display id: annotation2497192
version: 1
top level: BBa_K1946000
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1946000/annotation2497192/range2497192/1

https://synbiohub.org/public/igem/BBa_K1946000/annotation2497192/range2497192/1
type: Range
persistent identity: range2497192
display id: range2497192
version: 1
direction: direction/forward
top level: BBa_K1946000
start: 940
end: 942
orientation: inline

ChnR coding region
type: SequenceAnnotation
persistent identity: annotation2497189
display id: annotation2497189
version: 1
top level: BBa_K1946000
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1946000/annotation2497189/range2497189/1

https://synbiohub.org/public/igem/BBa_K1946000/annotation2497189/range2497189/1
type: Range
persistent identity: range2497189
display id: range2497189
version: 1
direction: direction/forward
top level: BBa_K1946000
start: 1
end: 942
orientation: inline

BBa_K1031444
type: ComponentDefinition
persistent identity: BBa K1031444
display id: BBa_K1031444
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: J23114-HcaR
creator: He Shuaixin
created: 2013-09-08T11:00:00Z
modified: 2015-05-08T01:08:47Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1338_
m user id: 0
owner id: 15843
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: 114-HcaR-TT
mutable notes: molecular cloning
mutable provenance: PCR genome E.coli
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: BBa_J23114
sequence annotation: HcaR
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K1031444_sequence/1

BBa_J23114
type: SequenceAnnotation
persistent identity: annotation2357463
display id: annotation2357463
version: 1
top level: BBa_K1031444
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1031444/annotation2357463/range2357463/1

https://synbiohub.org/public/igem/BBa_K1031444/annotation2357463/range2357463/1
type: Range
persistent identity: range2357463
display id: range2357463
version: 1
direction: direction/forward
top level: BBa_K1031444
start: 1
end: 35
orientation: inline

HcaR
type: SequenceAnnotation
persistent identity: annotation2357467
display id: annotation2357467
version: 1
top level: BBa_K1031444
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1031444/annotation2357467/range2357467/1

https://synbiohub.org/public/igem/BBa_K1031444/annotation2357467/range2357467/1
type: Range
persistent identity: range2357467
display id: range2357467
version: 1
direction: direction/forward
top level: BBa_K1031444
start: 62
end: 952
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2357465
display id: annotation2357465
version: 1
top level: BBa_K1031444
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1031444/annotation2357465/range2357465/1

https://synbiohub.org/public/igem/BBa_K1031444/annotation2357465/range2357465/1
type: Range
persistent identity: range2357465
display id: range2357465
version: 1
direction: direction/forward
top level: BBa_K1031444
start: 44
end: 55
orientation: inline

BBa_K2023015
type: ComponentDefinition
persistent identity: BBa K2023015
display id: BBa_K2023015
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: XylR coding device-GFP coding device with Pu
creator: C??lia Chenebault
created: 2016-10-13T11:00:00Z
modified: 2016-10-20T03:40:10Z
discontinued: false
dominant: false
group u list: _2490_
m user id: 30356
owner id: 30230
owning group id: 9
status: status/Planning
bookmark: false
mutable description: XylR coding device-GFP coding device with Pu
mutable notes: blabla
mutable provenance: XylR coding device (BBa_K2023002) and GFP coding device with Pu (BBa_K2023016)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Measurement
component: BBa_E0040
component: BBa_K2023002
component: BBa_I723020
component: BBa_B0015
component: BBa_B0034
sequence annotation: BBa_B0015
sequence annotation: BBa_I723020
sequence annotation: BBa_E0040
sequence annotation: BBa_K2023002
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K2023015_sequence/1

BBa_E0040
type: Component
persistent identity: component2518985
display id: component2518985
version: 1
top level: BBa_K2023015
access: public
definition: https://synbiohub.org/public/igem/BBa_E0040/1

BBa_K2023002
type: Component
persistent identity: component2518979
display id: component2518979
version: 1
top level: BBa_K2023015
access: public
definition: https://synbiohub.org/public/igem/BBa_K2023002/1

BBa_I723020
type: Component
persistent identity: component2518981
display id: component2518981
version: 1
top level: BBa_K2023015
access: public
definition: https://synbiohub.org/public/igem/BBa_I723020/1

BBa_B0015
type: Component
persistent identity: component2518992
display id: component2518992
version: 1
top level: BBa_K2023015
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_B0034
type: Component
persistent identity: component2518983
display id: component2518983
version: 1
top level: BBa_K2023015
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2518992
display id: annotation2518992
version: 1
top level: BBa_K2023015
location: https://synbiohub.org/public/igem/BBa_K2023015/annotation2518992/range2518992/1
component: BBa_B0015

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type: Range
persistent identity: range2518992
display id: range2518992
version: 1
direction: direction/reverse
top level: BBa_K2023015
start: 3326
end: 3454
orientation: inline

BBa_I723020
type: SequenceAnnotation
persistent identity: annotation2518981
display id: annotation2518981
version: 1
top level: BBa_K2023015
location: https://synbiohub.org/public/igem/BBa_K2023015/annotation2518981/range2518981/1
component: BBa_I723020

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type: Range
persistent identity: range2518981
display id: range2518981
version: 1
direction: direction/reverse
top level: BBa_K2023015
start: 2252
end: 2571
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation2518985
display id: annotation2518985
version: 1
top level: BBa_K2023015
location: https://synbiohub.org/public/igem/BBa_K2023015/annotation2518985/range2518985/1
component: BBa_E0040

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type: Range
persistent identity: range2518985
display id: range2518985
version: 1
direction: direction/reverse
top level: BBa_K2023015
start: 2598
end: 3317
orientation: inline

BBa_K2023002
type: SequenceAnnotation
persistent identity: annotation2518979
display id: annotation2518979
version: 1
top level: BBa_K2023015
location: https://synbiohub.org/public/igem/BBa_K2023015/annotation2518979/range2518979/1
component: BBa_K2023002

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type: Range
persistent identity: range2518979
display id: range2518979
version: 1
direction: direction/reverse
top level: BBa_K2023015
start: 1
end: 2243
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2518983
display id: annotation2518983
version: 1
top level: BBa_K2023015
location: https://synbiohub.org/public/igem/BBa_K2023015/annotation2518983/range2518983/1
component: BBa_B0034

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type: Range
persistent identity: range2518983
display id: range2518983
version: 1
direction: direction/reverse
top level: BBa_K2023015
start: 2580
end: 2591
orientation: inline

BBa_M36888
type: ComponentDefinition
persistent identity: BBa M36888
display id: BBa_M36888
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Sequence for sigma 32 transcription factor
creator: Shamik Mascharak, Justo Caballero and Sibel Sayiner
created: 2012-12-05T12:00:00Z
modified: 2015-05-08T01:14:06Z
discontinued: false
dominant: false
experience: experience/Issues
group u list: _848_
m user id: 0
owner id: 15030
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: The rpoH gene codes for sigma 32 (alternatively sigma H, RpoH) transcription factor, a component of RNA polymerase sigma 32 holoenzyme. This 32-kD stress factor belongs to the sigma-70 factor family and is involved in intracellular temperature regulation, initiating bacterial transcription of various chaperone proteins and proteases in response to sudden increases in ambient temperature. The gene is currently classified as essential, due to the fact that rpoH deletion mutants fail to grow at standard incubation temperatures. This part may be employed in heat shock-to-PoPs sensors by coupling expression of the rpoH gene to action of sigma 32-inducible promoters such as the htpG heat-shock promoter (part#: BBa_J45405). Transcriptional activation by rpoH transcripts is possible both within and without the sensor genetic circuit, as sigma factors can diffuse around the nucleus.
mutable notes: Designs incorporating this part into a feedback amplification loop must beware of likely rpoH-deletion mutants. This is due to overproduction of sigma 32 under leaky promoters such as htpG (BBa_J45405). Less deletion mutants in E. coli are noted when plasmids containing rpoH are cloned at higher temperature. Consider two facts before using this part. First, rpoH transcript activity is reduced under excess heat shock protein production. As such, feedback loops using rpoH should employ weaker bicistronic ribosome binding sites so as to optimize heat-shock response. Second, sigma 32 is turned over rapidly, with a half-life under 1 minute.
mutable provenance: The particular part described here is specific to the genome of W3110 strain E. coli.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: START
sequence annotation: STOP
sequence: https://synbiohub.org/public/igem/BBa_M36888_sequence/1

START
type: SequenceAnnotation
persistent identity: annotation2213636
display id: annotation2213636
version: 1
top level: BBa_M36888
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_M36888/annotation2213636/range2213636/1

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type: Range
persistent identity: range2213636
display id: range2213636
version: 1
direction: direction/forward
top level: BBa_M36888
start: 1
end: 3
orientation: inline

STOP
type: SequenceAnnotation
persistent identity: annotation2213640
display id: annotation2213640
version: 1
top level: BBa_M36888
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_M36888/annotation2213640/range2213640/1

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type: Range
persistent identity: range2213640
display id: range2213640
version: 1
direction: direction/forward
top level: BBa_M36888
start: 853
end: 861
orientation: inline

BBa_K606033
type: ComponentDefinition
persistent identity: BBa K606033
display id: BBa_K606033
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pVeg SpoVG T7amber double terminator.
creator: Axel S??guret
created: 2011-09-17T11:00:00Z
modified: 2015-05-08T01:12:51Z
discontinued: false
dominant: true
experience: experience/None
group u list: _778_
m user id: 0
owner id: 8931
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: T7 amber expression system in Bacillus subtilis.
mutable notes: biobrick assembly
mutable provenance: Bacillus subtilis
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K143053
component: BBa_B0015
component: BBa_K606032
sequence annotation: BBa_K606032
sequence annotation: BBa_B0015
sequence annotation: BBa_K143053
sequence: https://synbiohub.org/public/igem/BBa_K606033_sequence/1

BBa_K143053
type: Component
persistent identity: component2138050
display id: component2138050
version: 1
top level: BBa_K606033
access: public
definition: https://synbiohub.org/public/igem/BBa_K143053/1

BBa_B0015
type: Component
persistent identity: component2138063
display id: component2138063
version: 1
top level: BBa_K606033
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_K606032
type: Component
persistent identity: component2138056
display id: component2138056
version: 1
top level: BBa_K606033
access: public
definition: https://synbiohub.org/public/igem/BBa_K606032/1

BBa_K606032
type: SequenceAnnotation
persistent identity: annotation2138056
display id: annotation2138056
version: 1
top level: BBa_K606033
location: https://synbiohub.org/public/igem/BBa_K606033/annotation2138056/range2138056/1
component: BBa_K606032

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type: Range
persistent identity: range2138056
display id: range2138056
version: 1
direction: direction/reverse
top level: BBa_K606033
start: 124
end: 2778
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2138063
display id: annotation2138063
version: 1
top level: BBa_K606033
location: https://synbiohub.org/public/igem/BBa_K606033/annotation2138063/range2138063/1
component: BBa_B0015

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type: Range
persistent identity: range2138063
display id: range2138063
version: 1
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BBa_K143053
type: SequenceAnnotation
persistent identity: annotation2138050
display id: annotation2138050
version: 1
top level: BBa_K606033
location: https://synbiohub.org/public/igem/BBa_K606033/annotation2138050/range2138050/1
component: BBa_K143053

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persistent identity: range2138050
display id: range2138050
version: 1
direction: direction/reverse
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start: 1
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orientation: inline

lasR
type: ComponentDefinition
persistent identity: BBa C0179
display id: BBa_C0179
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: lasR activator from P. aeruginosa PAO1(no LVA)
creator: jcbraff
created: 2004-05-26T11:00:00Z
modified: 2015-08-31T04:07:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_1_
m user id: 0
owner id: 61
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: same as C0079 except no LVA tag
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_C0179_sequence/1

BBa_K1486002
type: ComponentDefinition
persistent identity: BBa K1486002
display id: BBa_K1486002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Arabinose promoter + sfGFP CpxR (Nterm)
creator: iGem EPFL 2014
created: 2014-08-11T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part is composed of an arabinose-inducible promoter for superfolded GFP attached by a linker to CpxR (on the N terminus of CpxR).
mutable notes: We had to test both attachments, N and C terminus of the CpxR.
mutable provenance: The arabinose promoter and the sfGFP were obtained from the registry, while the CpxR was obtained from the E coli genome.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K1486003
component: BBa_K1486000
component: BBa_I746916
component: BBa_B0034
component: BBa_I0500
sequence annotation: BBa_I746916
sequence annotation: BBa_I0500
sequence annotation: BBa_B0034
sequence annotation: BBa_K1486003
sequence annotation: BBa_K1486000
sequence: https://synbiohub.org/public/igem/BBa_K1486002_sequence/1

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type: Component
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display id: component2382064
version: 1
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access: public
definition: https://synbiohub.org/public/igem/BBa_K1486003/1

BBa_K1486000
type: Component
persistent identity: component2382068
display id: component2382068
version: 1
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access: public
definition: BBa_K1486000

BBa_I746916
type: Component
persistent identity: component2382063
display id: component2382063
version: 1
top level: BBa_K1486002
access: public
definition: https://synbiohub.org/public/igem/BBa_I746916/1

BBa_B0034
type: Component
persistent identity: component2382059
display id: component2382059
version: 1
top level: BBa_K1486002
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_I0500
type: Component
persistent identity: component2382057
display id: component2382057
version: 1
top level: BBa_K1486002
access: public
definition: https://synbiohub.org/public/igem/BBa_I0500/1

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type: SequenceAnnotation
persistent identity: annotation2382063
display id: annotation2382063
version: 1
top level: BBa_K1486002
location: https://synbiohub.org/public/igem/BBa_K1486002/annotation2382063/range2382063/1
component: BBa_I746916

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display id: annotation2382057
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type: Range
persistent identity: range2382057
display id: range2382057
version: 1
direction: direction/reverse
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orientation: inline

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type: SequenceAnnotation
persistent identity: annotation2382059
display id: annotation2382059
version: 1
top level: BBa_K1486002
location: https://synbiohub.org/public/igem/BBa_K1486002/annotation2382059/range2382059/1
component: BBa_B0034

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persistent identity: range2382059
display id: range2382059
version: 1
direction: direction/reverse
top level: BBa_K1486002
start: 1211
end: 1222
orientation: inline

BBa_K1486003
type: SequenceAnnotation
persistent identity: annotation2382064
display id: annotation2382064
version: 1
top level: BBa_K1486002
location: https://synbiohub.org/public/igem/BBa_K1486002/annotation2382064/range2382064/1
component: BBa_K1486003

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type: Range
persistent identity: range2382064
display id: range2382064
version: 1
direction: direction/reverse
top level: BBa_K1486002
start: 1943
end: 1966
orientation: inline

BBa_K1486000
type: SequenceAnnotation
persistent identity: annotation2382068
display id: annotation2382068
version: 1
top level: BBa_K1486002
location: https://synbiohub.org/public/igem/BBa_K1486002/annotation2382068/range2382068/1
component: BBa_K1486000

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type: Range
persistent identity: range2382068
display id: range2382068
version: 1
direction: direction/reverse
top level: BBa_K1486002
start: 1967
end: 2668
orientation: inline

BBa_K1031111
type: ComponentDefinition
persistent identity: BBa K1031111
display id: BBa_K1031111
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: J23109-CapR-Terminator
creator: He Shuaixin
created: 2013-09-08T11:00:00Z
modified: 2015-05-08T01:08:47Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1338_
m user id: 0
owner id: 15843
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: 109-CapR-TT
mutable notes: molecular cloning
mutable provenance: synthesis Pseudomonas putida
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: J23109
sequence annotation: CapR
sequence annotation: B0015
sequence: https://synbiohub.org/public/igem/BBa_K1031111_sequence/1

J23109
type: SequenceAnnotation
persistent identity: annotation2361309
display id: annotation2361309
version: 1
top level: BBa_K1031111
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1031111/annotation2361309/range2361309/1

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type: Range
persistent identity: range2361309
display id: range2361309
version: 1
direction: direction/forward
top level: BBa_K1031111
start: 1
end: 35
orientation: inline

CapR
type: SequenceAnnotation
persistent identity: annotation2361310
display id: annotation2361310
version: 1
top level: BBa_K1031111
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1031111/annotation2361310/range2361310/1

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persistent identity: range2361310
display id: range2361310
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direction: direction/forward
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start: 62
end: 1753
orientation: inline

B0015
type: SequenceAnnotation
persistent identity: annotation2361311
display id: annotation2361311
version: 1
top level: BBa_K1031111
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1031111/annotation2361311/range2361311/1

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type: Range
persistent identity: range2361311
display id: range2361311
version: 1
direction: direction/forward
top level: BBa_K1031111
start: 1760
end: 1888
orientation: inline

BBa_K1486042
type: ComponentDefinition
persistent identity: BBa K1486042
display id: BBa_K1486042
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Leucine Zipper
creator: EPFL iGem team 2014
created: 2014-10-01T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: To edit
mutable notes: To edit
mutable provenance: To edit
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence: https://synbiohub.org/public/igem/BBa_K1486042_sequence/1

BBa_K1486010
type: ComponentDefinition
persistent identity: BBa K1486010
display id: BBa_K1486010
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CxpR & Split IFP1.4 [Nterm + Cterm]
creator: iGem EPFL 2014
created: 2014-08-26T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part has two fused proteins downstream of an arabinose-inducible promoter: CpxR-IFP[1] and CpxR-IFP[2]. This construct was made in all orientations: - CpxR-IFP[1] + CpxR-IFP[2] (BBa_K1286008) - IFP[1]-CpxR + CpxR-IFP[2] (BBa_K1486010) - CpxR-IFP[1] + IFP[2]-CpxR (BBa_K1486011)
mutable notes: All orientations were made, so all dimerizations could be tested (see main page for biobrick numbers). Flexible linkers were used for each of the fused proteins, and an RBS was put before each one of them.
mutable provenance: From Michnick Article. To Edit
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K1486000
component: BBa_B0034
component: BBa_K1486007
component: BBa_I0500
component: BBa_K1486006
component: BBa_K1486004
sequence annotation: BBa_K1486006
sequence annotation: BBa_K1486004
sequence annotation: BBa_K1486000
sequence annotation: BBa_B0034
sequence annotation: BBa_I0500
sequence annotation: BBa_K1486007
sequence: https://synbiohub.org/public/igem/BBa_K1486010_sequence/1

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display id: component2424995
version: 1
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access: public
definition: BBa_K1486000

BBa_B0034
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persistent identity: component2424997
display id: component2424997
version: 1
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access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K1486007
type: Component
persistent identity: component2425006
display id: component2425006
version: 1
top level: BBa_K1486010
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486007/1

BBa_I0500
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persistent identity: component2424984
display id: component2424984
version: 1
top level: BBa_K1486010
access: public
definition: https://synbiohub.org/public/igem/BBa_I0500/1

BBa_K1486006
type: Component
persistent identity: component2424990
display id: component2424990
version: 1
top level: BBa_K1486010
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486006/1

BBa_K1486000
type: Component
persistent identity: component2425001
display id: component2425001
version: 1
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access: public
definition: BBa_K1486000

BBa_B0034
type: Component
persistent identity: component2424986
display id: component2424986
version: 1
top level: BBa_K1486010
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K1486004
type: Component
persistent identity: component2425002
display id: component2425002
version: 1
top level: BBa_K1486010
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486004/1

BBa_K1486004
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persistent identity: component2424991
display id: component2424991
version: 1
top level: BBa_K1486010
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486004/1

BBa_K1486006
type: SequenceAnnotation
persistent identity: annotation2424990
display id: annotation2424990
version: 1
top level: BBa_K1486010
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persistent identity: range2424990
display id: range2424990
version: 1
direction: direction/reverse
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start: 1223
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orientation: inline

BBa_K1486004
type: SequenceAnnotation
persistent identity: annotation2424991
display id: annotation2424991
version: 1
top level: BBa_K1486010
location: https://synbiohub.org/public/igem/BBa_K1486010/annotation2424991/range2424991/1
component: BBa_K1486004

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type: Range
persistent identity: range2424991
display id: range2424991
version: 1
direction: direction/reverse
top level: BBa_K1486010
start: 1628
end: 1657
orientation: inline

BBa_K1486000
type: SequenceAnnotation
persistent identity: annotation2424995
display id: annotation2424995
version: 1
top level: BBa_K1486010
location: https://synbiohub.org/public/igem/BBa_K1486010/annotation2424995/range2424995/1
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persistent identity: range2424995
display id: range2424995
version: 1
direction: direction/reverse
top level: BBa_K1486010
start: 1658
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BBa_B0034
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persistent identity: annotation2424997
display id: annotation2424997
version: 1
top level: BBa_K1486010
location: https://synbiohub.org/public/igem/BBa_K1486010/annotation2424997/range2424997/1
component: BBa_B0034

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persistent identity: range2424997
display id: range2424997
version: 1
direction: direction/reverse
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start: 2360
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persistent identity: annotation2425001
display id: annotation2425001
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persistent identity: range2425001
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direction: direction/reverse
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display id: annotation2424986
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persistent identity: range2424986
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persistent identity: annotation2425002
display id: annotation2425002
version: 1
top level: BBa_K1486010
location: https://synbiohub.org/public/igem/BBa_K1486010/annotation2425002/range2425002/1
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persistent identity: range2425002
display id: range2425002
version: 1
direction: direction/reverse
top level: BBa_K1486010
start: 3074
end: 3103
orientation: inline

BBa_K1486007
type: SequenceAnnotation
persistent identity: annotation2425006
display id: annotation2425006
version: 1
top level: BBa_K1486010
location: https://synbiohub.org/public/igem/BBa_K1486010/annotation2425006/range2425006/1
component: BBa_K1486007

https://synbiohub.org/public/igem/BBa_K1486010/annotation2425006/range2425006/1
type: Range
persistent identity: range2425006
display id: range2425006
version: 1
direction: direction/reverse
top level: BBa_K1486010
start: 3104
end: 3688
orientation: inline

LuxO D47A
type: ComponentDefinition
persistent identity: BBa K131023
display id: BBa_K131023
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LuxO D47A, Vibrio harveyi
creator: Thane Kubik
created: 2008-10-27T12:00:00Z
modified: 2015-05-08T01:09:47Z
discontinued: false
dominant: true
experience: experience/None
group u list: _259_
m user id: 0
owner id: 3190
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: LuxO D47A is a inactive mutant of LuxO.
mutable notes: Plasmid containing LuxO D47A (pJAF544) was obtained from Bassler lab (Freeman and Bassler, 1999).
mutable provenance: Jeremy A. Freeman and Bonnie L. Bassler. A genetic analysis of the function of LuxO, a two-component response regulator involved in quorum sensing in Vibrio harveyi. 1999a. Molecular Microbiology. 31(2), 665-677.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: D47A
sequence annotation: Stop
sequence annotation: Start
sequence: https://synbiohub.org/public/igem/BBa_K131023_sequence/1

D47A
type: SequenceAnnotation
persistent identity: annotation1990657
display id: annotation1990657
version: 1
top level: LuxO D47A
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K131023/annotation1990657/range1990657/1

https://synbiohub.org/public/igem/BBa_K131023/annotation1990657/range1990657/1
type: Range
persistent identity: range1990657
display id: range1990657
version: 1
direction: direction/forward
top level: LuxO D47A
start: 139
end: 141
orientation: inline

Stop
type: SequenceAnnotation
persistent identity: annotation1990659
display id: annotation1990659
version: 1
top level: LuxO D47A
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K131023/annotation1990659/range1990659/1

https://synbiohub.org/public/igem/BBa_K131023/annotation1990659/range1990659/1
type: Range
persistent identity: range1990659
display id: range1990659
version: 1
direction: direction/forward
top level: LuxO D47A
start: 1360
end: 1362
orientation: inline

Start
type: SequenceAnnotation
persistent identity: annotation1990658
display id: annotation1990658
version: 1
top level: LuxO D47A
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K131023/annotation1990658/range1990658/1

https://synbiohub.org/public/igem/BBa_K131023/annotation1990658/range1990658/1
type: Range
persistent identity: range1990658
display id: range1990658
version: 1
direction: direction/forward
top level: LuxO D47A
start: 1
end: 3
orientation: inline

BBa_K1486013
type: ComponentDefinition
persistent identity: BBa K1486013
display id: BBa_K1486013
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CpxR-IFP[2] under pBAD
creator: iGem EPFL 2014
created: 2014-08-31T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part has a fused protein downstream of an arabinose-inducible promoter: CpxR-IFP[2]. Biobrick BBa_K1486012 has CpxR-IFP[1] with an arabinose promoter. This construct was made in both orientations: BBa_K1486015 is IFP[2]-CpxR (with an arabinose promoter).
mutable notes: Both orientations were made, as well as for IFP[1] so all dimerizations could be tested (see main page for biobrick numbers). Flexible linkers were used to fuse both proteins, and an RBS was put before each one of them.
mutable provenance: to edit
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K1486007
component: BBa_I0500
component: BBa_K1486004
component: BBa_K1486000
component: BBa_B0034
sequence annotation: BBa_K1486000
sequence annotation: BBa_B0034
sequence annotation: BBa_I0500
sequence annotation: BBa_K1486007
sequence annotation: BBa_K1486004
sequence: https://synbiohub.org/public/igem/BBa_K1486013_sequence/1

BBa_K1486007
type: Component
persistent identity: component2382308
display id: component2382308
version: 1
top level: BBa_K1486013
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486007/1

BBa_I0500
type: Component
persistent identity: component2382297
display id: component2382297
version: 1
top level: BBa_K1486013
access: public
definition: https://synbiohub.org/public/igem/BBa_I0500/1

BBa_K1486004
type: Component
persistent identity: component2382304
display id: component2382304
version: 1
top level: BBa_K1486013
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486004/1

BBa_K1486000
type: Component
persistent identity: component2382303
display id: component2382303
version: 1
top level: BBa_K1486013
access: public
definition: BBa_K1486000

BBa_B0034
type: Component
persistent identity: component2382299
display id: component2382299
version: 1
top level: BBa_K1486013
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K1486000
type: SequenceAnnotation
persistent identity: annotation2382303
display id: annotation2382303
version: 1
top level: BBa_K1486013
location: https://synbiohub.org/public/igem/BBa_K1486013/annotation2382303/range2382303/1
component: BBa_K1486000

https://synbiohub.org/public/igem/BBa_K1486013/annotation2382303/range2382303/1
type: Range
persistent identity: range2382303
display id: range2382303
version: 1
direction: direction/reverse
top level: BBa_K1486013
start: 1223
end: 1924
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2382299
display id: annotation2382299
version: 1
top level: BBa_K1486013
location: https://synbiohub.org/public/igem/BBa_K1486013/annotation2382299/range2382299/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1486013/annotation2382299/range2382299/1
type: Range
persistent identity: range2382299
display id: range2382299
version: 1
direction: direction/reverse
top level: BBa_K1486013
start: 1211
end: 1222
orientation: inline

BBa_I0500
type: SequenceAnnotation
persistent identity: annotation2382297
display id: annotation2382297
version: 1
top level: BBa_K1486013
location: https://synbiohub.org/public/igem/BBa_K1486013/annotation2382297/range2382297/1
component: BBa_I0500

https://synbiohub.org/public/igem/BBa_K1486013/annotation2382297/range2382297/1
type: Range
persistent identity: range2382297
display id: range2382297
version: 1
direction: direction/reverse
top level: BBa_K1486013
start: 1
end: 1210
orientation: inline

BBa_K1486007
type: SequenceAnnotation
persistent identity: annotation2382308
display id: annotation2382308
version: 1
top level: BBa_K1486013
location: https://synbiohub.org/public/igem/BBa_K1486013/annotation2382308/range2382308/1
component: BBa_K1486007

https://synbiohub.org/public/igem/BBa_K1486013/annotation2382308/range2382308/1
type: Range
persistent identity: range2382308
display id: range2382308
version: 1
direction: direction/reverse
top level: BBa_K1486013
start: 1955
end: 2533
orientation: inline

BBa_K1486004
type: SequenceAnnotation
persistent identity: annotation2382304
display id: annotation2382304
version: 1
top level: BBa_K1486013
location: https://synbiohub.org/public/igem/BBa_K1486013/annotation2382304/range2382304/1
component: BBa_K1486004

https://synbiohub.org/public/igem/BBa_K1486013/annotation2382304/range2382304/1
type: Range
persistent identity: range2382304
display id: range2382304
version: 1
direction: direction/reverse
top level: BBa_K1486013
start: 1925
end: 1954
orientation: inline

BBa_K1031443
type: ComponentDefinition
persistent identity: BBa K1031443
display id: BBa_K1031443
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: J23113-HcaR
creator: He Shuaixin
created: 2013-09-08T11:00:00Z
modified: 2015-05-08T01:08:47Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1338_
m user id: 0
owner id: 15843
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: 113-HcaR-TT
mutable notes: molecular cloning
mutable provenance: PCR genome E.coli
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: HcaR
sequence annotation: BBa_B0034
sequence annotation: BBa_J23113
sequence: https://synbiohub.org/public/igem/BBa_K1031443_sequence/1

HcaR
type: SequenceAnnotation
persistent identity: annotation2357461
display id: annotation2357461
version: 1
top level: BBa_K1031443
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1031443/annotation2357461/range2357461/1

https://synbiohub.org/public/igem/BBa_K1031443/annotation2357461/range2357461/1
type: Range
persistent identity: range2357461
display id: range2357461
version: 1
direction: direction/forward
top level: BBa_K1031443
start: 62
end: 952
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2357460
display id: annotation2357460
version: 1
top level: BBa_K1031443
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1031443/annotation2357460/range2357460/1

https://synbiohub.org/public/igem/BBa_K1031443/annotation2357460/range2357460/1
type: Range
persistent identity: range2357460
display id: range2357460
version: 1
direction: direction/forward
top level: BBa_K1031443
start: 44
end: 55
orientation: inline

BBa_J23113
type: SequenceAnnotation
persistent identity: annotation2357459
display id: annotation2357459
version: 1
top level: BBa_K1031443
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1031443/annotation2357459/range2357459/1

https://synbiohub.org/public/igem/BBa_K1031443/annotation2357459/range2357459/1
type: Range
persistent identity: range2357459
display id: range2357459
version: 1
direction: direction/forward
top level: BBa_K1031443
start: 1
end: 35
orientation: inline

BBa_K082006
type: ComponentDefinition
persistent identity: BBa K082006
display id: BBa_K082006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LuxR-G2F
creator: Yanting Xue , He Shen
created: 2008-10-27T12:00:00Z
modified: 2015-05-08T01:08:35Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _228_
m user id: 0
owner id: 1560
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: none
mutable notes: None
mutable provenance: None
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: BBa_K082006
sequence: https://synbiohub.org/public/igem/BBa_K082006_sequence/1

BBa_K082006
type: SequenceAnnotation
persistent identity: annotation1993281
display id: annotation1993281
version: 1
top level: BBa_K082006
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K082006/annotation1993281/range1993281/1

https://synbiohub.org/public/igem/BBa_K082006/annotation1993281/range1993281/1
type: Range
persistent identity: range1993281
display id: range1993281
version: 1
direction: direction/reverse
top level: BBa_K082006
start: 1
end: 753
orientation: inline

BBa_K1031441
type: ComponentDefinition
persistent identity: BBa K1031441
display id: BBa_K1031441
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: J23106-HcaR
creator: He Shuaixin
created: 2013-09-08T11:00:00Z
modified: 2015-05-08T01:08:47Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1338_
m user id: 0
owner id: 15843
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: 106-HcaR-TT
mutable notes: molecular cloning
mutable provenance: PCR genome E.coli
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: BBa_B0034
sequence annotation: HcaR
sequence annotation: BBa_J23106
sequence: https://synbiohub.org/public/igem/BBa_K1031441_sequence/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2357454
display id: annotation2357454
version: 1
top level: BBa_K1031441
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1031441/annotation2357454/range2357454/1

https://synbiohub.org/public/igem/BBa_K1031441/annotation2357454/range2357454/1
type: Range
persistent identity: range2357454
display id: range2357454
version: 1
direction: direction/forward
top level: BBa_K1031441
start: 44
end: 55
orientation: inline

HcaR
type: SequenceAnnotation
persistent identity: annotation2357455
display id: annotation2357455
version: 1
top level: BBa_K1031441
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1031441/annotation2357455/range2357455/1

https://synbiohub.org/public/igem/BBa_K1031441/annotation2357455/range2357455/1
type: Range
persistent identity: range2357455
display id: range2357455
version: 1
direction: direction/forward
top level: BBa_K1031441
start: 62
end: 952
orientation: inline

BBa_J23106
type: SequenceAnnotation
persistent identity: annotation2357453
display id: annotation2357453
version: 1
top level: BBa_K1031441
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1031441/annotation2357453/range2357453/1

https://synbiohub.org/public/igem/BBa_K1031441/annotation2357453/range2357453/1
type: Range
persistent identity: range2357453
display id: range2357453
version: 1
direction: direction/forward
top level: BBa_K1031441
start: 1
end: 35
orientation: inline

BBa_K266002
type: ComponentDefinition
persistent identity: BBa K266002
display id: BBa_K266002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LasR + Term
creator: UNAM FCIENCIAS-CINVESTAV IRA
created: 2009-10-12T11:00:00Z
modified: 2015-05-08T01:11:43Z
discontinued: false
dominant: false
experience: experience/None
group u list: _363_
m user id: 0
owner id: 1955
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: LasR coding region and a double terminator
mutable notes: None
mutable provenance: The biobricks BBa_C0079 and BBa_B0015 where used for this biobrick.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_B0015
component: BBa_C0079
sequence annotation: BBa_C0079
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_K266002_sequence/1

BBa_B0015
type: Component
persistent identity: component2246671
display id: component2246671
version: 1
top level: BBa_K266002
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_C0079
type: Component
persistent identity: component2246664
display id: component2246664
version: 1
top level: BBa_K266002
access: public
definition: lasr

BBa_C0079
type: SequenceAnnotation
persistent identity: annotation2246664
display id: annotation2246664
version: 1
top level: BBa_K266002
location: https://synbiohub.org/public/igem/BBa_K266002/annotation2246664/range2246664/1
component: BBa_C0079

https://synbiohub.org/public/igem/BBa_K266002/annotation2246664/range2246664/1
type: Range
persistent identity: range2246664
display id: range2246664
version: 1
direction: direction/reverse
top level: BBa_K266002
start: 1
end: 781
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2246671
display id: annotation2246671
version: 1
top level: BBa_K266002
location: https://synbiohub.org/public/igem/BBa_K266002/annotation2246671/range2246671/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K266002/annotation2246671/range2246671/1
type: Range
persistent identity: range2246671
display id: range2246671
version: 1
direction: direction/reverse
top level: BBa_K266002
start: 790
end: 918
orientation: inline

BBa_K1067009
type: ComponentDefinition
persistent identity: BBa K1067009
display id: BBa_K1067009
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Periplasm transport visualization device
creator: Kristian Davidsen
created: 2013-09-27T11:00:00Z
modified: 2015-05-08T01:09:00Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1376_
m user id: 0
owner id: 16028
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: ggg
mutable notes: ggg
mutable provenance: ggg
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
sequence annotation: GFP SF
sequence annotation: stop
sequence annotation: TAT signal peptide
sequence annotation: RFP mCherry
sequence annotation: BBa_K1067001
sequence annotation: CRP binding site
sequence annotation: araO2
sequence annotation: BBa_K1067007
sequence annotation: rbs
sequence annotation: start
sequence annotation: araO1R
sequence annotation: araC
sequence annotation: Tight pBAD promoter
sequence annotation: -10
sequence annotation: araO1L
sequence annotation: Gap
sequence annotation: araI2
sequence annotation: araI1
sequence annotation: -35
sequence: https://synbiohub.org/public/igem/BBa_K1067009_sequence/1

GFP SF
type: SequenceAnnotation
persistent identity: annotation2366516
display id: annotation2366516
version: 1
top level: BBa_K1067009
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1067009/annotation2366516/range2366516/1

https://synbiohub.org/public/igem/BBa_K1067009/annotation2366516/range2366516/1
type: Range
persistent identity: range2366516
display id: range2366516
version: 1
direction: direction/forward
top level: BBa_K1067009
start: 1357
end: 2070
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2366522
display id: annotation2366522
version: 1
top level: BBa_K1067009
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1067009/annotation2366522/range2366522/1

https://synbiohub.org/public/igem/BBa_K1067009/annotation2366522/range2366522/1
type: Range
persistent identity: range2366522
display id: range2366522
version: 1
direction: direction/forward
top level: BBa_K1067009
start: 2799
end: 2801
orientation: inline

TAT signal peptide
type: SequenceAnnotation
persistent identity: annotation2366514
display id: annotation2366514
version: 1
top level: BBa_K1067009
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1067009/annotation2366514/range2366514/1

https://synbiohub.org/public/igem/BBa_K1067009/annotation2366514/range2366514/1
type: Range
persistent identity: range2366514
display id: range2366514
version: 1
direction: direction/forward
top level: BBa_K1067009
start: 1240
end: 1356
orientation: inline

RFP mCherry
type: SequenceAnnotation
persistent identity: annotation2366521
display id: annotation2366521
version: 1
top level: BBa_K1067009
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1067009/annotation2366521/range2366521/1

https://synbiohub.org/public/igem/BBa_K1067009/annotation2366521/range2366521/1
type: Range
persistent identity: range2366521
display id: range2366521
version: 1
direction: direction/forward
top level: BBa_K1067009
start: 2091
end: 2801
orientation: inline

BBa_K1067001
type: SequenceAnnotation
persistent identity: annotation2366523
display id: annotation2366523
version: 1
top level: BBa_K1067009
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1067009/annotation2366523/range2366523/1

https://synbiohub.org/public/igem/BBa_K1067009/annotation2366523/range2366523/1
type: Range
persistent identity: range2366523
display id: range2366523
version: 1
direction: direction/reverse
top level: BBa_K1067009
start: 1218
end: 2801
orientation: inline

CRP binding site
type: SequenceAnnotation
persistent identity: annotation2366507
display id: annotation2366507
version: 1
top level: BBa_K1067009
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1067009/annotation2366507/range2366507/1

https://synbiohub.org/public/igem/BBa_K1067009/annotation2366507/range2366507/1
type: Range
persistent identity: range2366507
display id: range2366507
version: 1
direction: direction/forward
top level: BBa_K1067009
start: 1087
end: 1108
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2366517
display id: annotation2366517
version: 1
top level: BBa_K1067009
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1067009/annotation2366517/range2366517/1

https://synbiohub.org/public/igem/BBa_K1067009/annotation2366517/range2366517/1
type: Range
persistent identity: range2366517
display id: range2366517
version: 1
direction: direction/forward
top level: BBa_K1067009
start: 2068
end: 2070
orientation: inline

araO2
type: SequenceAnnotation
persistent identity: annotation2366504
display id: annotation2366504
version: 1
top level: BBa_K1067009
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1067009/annotation2366504/range2366504/1

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type: Range
persistent identity: range2366504
display id: range2366504
version: 1
direction: direction/forward
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start: 908
end: 924
orientation: inline

BBa_K1067007
type: SequenceAnnotation
persistent identity: annotation2366513
display id: annotation2366513
version: 1
top level: BBa_K1067009
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1067009/annotation2366513/range2366513/1

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type: Range
persistent identity: range2366513
display id: range2366513
version: 1
direction: direction/reverse
top level: BBa_K1067009
start: 1
end: 1209
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation2366519
display id: annotation2366519
version: 1
top level: BBa_K1067009
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1067009/annotation2366519/range2366519/1

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type: Range
persistent identity: range2366519
display id: range2366519
version: 1
direction: direction/forward
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start: 2079
end: 2083
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2366515
display id: annotation2366515
version: 1
top level: BBa_K1067009
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1067009/annotation2366515/range2366515/1

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type: Range
persistent identity: range2366515
display id: range2366515
version: 1
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start: 1240
end: 1242
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start
type: SequenceAnnotation
persistent identity: annotation2366520
display id: annotation2366520
version: 1
top level: BBa_K1067009
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1067009/annotation2366520/range2366520/1

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type: Range
persistent identity: range2366520
display id: range2366520
version: 1
direction: direction/forward
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start: 2091
end: 2093
orientation: inline

araO1R
type: SequenceAnnotation
persistent identity: annotation2366506
display id: annotation2366506
version: 1
top level: BBa_K1067009
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1067009/annotation2366506/range2366506/1

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type: Range
persistent identity: range2366506
display id: range2366506
version: 1
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start: 1066
end: 1082
orientation: inline

araC
type: SequenceAnnotation
persistent identity: annotation2366503
display id: annotation2366503
version: 1
top level: BBa_K1067009
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1067009/annotation2366503/range2366503/1

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type: Range
persistent identity: range2366503
display id: range2366503
version: 1
direction: direction/forward
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start: 1
end: 880
orientation: inline

Tight pBAD promoter
type: SequenceAnnotation
persistent identity: annotation2366510
display id: annotation2366510
version: 1
top level: BBa_K1067009
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1067009/annotation2366510/range2366510/1

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persistent identity: range2366510
display id: range2366510
version: 1
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start: 1153
end: 1189
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-10
type: SequenceAnnotation
persistent identity: annotation2366512
display id: annotation2366512
version: 1
top level: BBa_K1067009
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1067009/annotation2366512/range2366512/1

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type: Range
persistent identity: range2366512
display id: range2366512
version: 1
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start: 1177
end: 1182
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araO1L
type: SequenceAnnotation
persistent identity: annotation2366505
display id: annotation2366505
version: 1
top level: BBa_K1067009
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1067009/annotation2366505/range2366505/1

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type: Range
persistent identity: range2366505
display id: range2366505
version: 1
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start: 1045
end: 1061
orientation: inline

Gap
type: SequenceAnnotation
persistent identity: annotation2366518
display id: annotation2366518
version: 1
top level: BBa_K1067009
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1067009/annotation2366518/range2366518/1

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type: Range
persistent identity: range2366518
display id: range2366518
version: 1
direction: direction/forward
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start: 2071
end: 2090
orientation: inline

araI2
type: SequenceAnnotation
persistent identity: annotation2366509
display id: annotation2366509
version: 1
top level: BBa_K1067009
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1067009/annotation2366509/range2366509/1

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type: Range
persistent identity: range2366509
display id: range2366509
version: 1
direction: direction/forward
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start: 1140
end: 1157
orientation: inline

araI1
type: SequenceAnnotation
persistent identity: annotation2366508
display id: annotation2366508
version: 1
top level: BBa_K1067009
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1067009/annotation2366508/range2366508/1

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type: Range
persistent identity: range2366508
display id: range2366508
version: 1
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start: 1119
end: 1135
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-35
type: SequenceAnnotation
persistent identity: annotation2366511
display id: annotation2366511
version: 1
top level: BBa_K1067009
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1067009/annotation2366511/range2366511/1

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type: Range
persistent identity: range2366511
display id: range2366511
version: 1
direction: direction/forward
top level: BBa_K1067009
start: 1153
end: 1158
orientation: inline

BBa_K1520021
type: ComponentDefinition
persistent identity: BBa K1520021
display id: BBa_K1520021
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Pcons2-rbs-lacI-Ter-Plac-rbs-luxI-Ter-Pcons2-rbs-luxR-Ter-Prlux-rbs-rfp-Ter
creator: yue wu
created: 2014-09-21T11:00:00Z
modified: 2015-05-08T01:10:49Z
discontinued: false
dominant: false
group u list: _1902_
m user id: 0
owner id: 21318
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Plasmid in positive feedback verification: Pcons2-rbs-lacI-Ter-Plac-rbs-luxI-Ter-Pcons2-rbs-luxR-Ter-Prlux-rbs-rfp-Ter. It is a control plasmid for positive feedback circuit. It will produce rfp when there is enough IPTG.
mutable notes: None
mutable provenance: Pcons2, lacI, Plac, luxI, Pcons2, luxR, Prlux, rfp, Ter
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1520010
component: BBa_K1520020
sequence annotation: BBa_K1520010
sequence annotation: BBa_K1520020
sequence: https://synbiohub.org/public/igem/BBa_K1520021_sequence/1

BBa_K1520010
type: Component
persistent identity: component2385150
display id: component2385150
version: 1
top level: BBa_K1520021
access: public
definition: https://synbiohub.org/public/igem/BBa_K1520010/1

BBa_K1520020
type: Component
persistent identity: component2385142
display id: component2385142
version: 1
top level: BBa_K1520021
access: public
definition: BBa_K1520020

BBa_K1520010
type: SequenceAnnotation
persistent identity: annotation2385150
display id: annotation2385150
version: 1
top level: BBa_K1520021
location: https://synbiohub.org/public/igem/BBa_K1520021/annotation2385150/range2385150/1
component: BBa_K1520010

https://synbiohub.org/public/igem/BBa_K1520021/annotation2385150/range2385150/1
type: Range
persistent identity: range2385150
display id: range2385150
version: 1
direction: direction/reverse
top level: BBa_K1520021
start: 3361
end: 4292
orientation: inline

BBa_K1520020
type: SequenceAnnotation
persistent identity: annotation2385142
display id: annotation2385142
version: 1
top level: BBa_K1520021
location: https://synbiohub.org/public/igem/BBa_K1520021/annotation2385142/range2385142/1
component: BBa_K1520020

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type: Range
persistent identity: range2385142
display id: range2385142
version: 1
direction: direction/reverse
top level: BBa_K1520021
start: 1
end: 3352
orientation: inline

BBa_K1315005
type: ComponentDefinition
persistent identity: BBa K1315005
display id: BBa_K1315005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: The clp gene from Xanthomonas campestris
creator: Dimitrios Michailidis
created: 2014-09-24T11:00:00Z
modified: 2015-05-08T01:09:50Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1690_
m user id: 0
owner id: 21812
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: c-di-GMP Repressed DNA Binding Protein (Clp) Quorum sensing and bacterial cell signalling are mechanisms known to be utilised by bacteria to regulate their virulence. The RpfC-RpfG sensory system has been found to activate the production of virulence factors like extracellular enzymes and polysaccharides in Xanthomonas campestris. It has been also suggested that this system is involved in linking environmental stimuli, including DSF, with pathogenicity in Xanthomonas campestris. DSF is a cis-unsaturated fatty acid from the DSF family. The RpfC-RpfG two-component system is the most well understood one and is present in all Xanthomonads. http://www.ncbi.nlm.nih.gov/pubmed/?term=r+ryan+dsf+family http://www.ncbi.nlm.nih.gov/pubmed/?term=A+two-component+system+involving+an+HD-GYP+domain+protein+links+cell%E2%80%93cell+signalling+to+pathogenicity+gene+expression+in+Xanthomonas+campestris
mutable notes: The sequence had 1 PstI site that was mutated out by site-directed mutagenesis.
mutable provenance: The source of this part is Xanthomonas campestris pv. campestris 8004 genomic DNA which was provided by the Robert Ryan lab in the Molecular Microbiology department of the University of Dundee, UK.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Clp
sequence: https://synbiohub.org/public/igem/BBa_K1315005_sequence/1

Clp
type: SequenceAnnotation
persistent identity: annotation2386527
display id: annotation2386527
version: 1
top level: BBa_K1315005
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1315005/annotation2386527/range2386527/1

https://synbiohub.org/public/igem/BBa_K1315005/annotation2386527/range2386527/1
type: Range
persistent identity: range2386527
display id: range2386527
version: 1
direction: direction/forward
top level: BBa_K1315005
start: 1
end: 696
orientation: inline

BBa_K1486056
type: ComponentDefinition
persistent identity: BBa K1486056
display id: BBa_K1486056
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CxpR & Split IFP1.4 [Cterm + Cterm][2]
creator: iGem EPFL 2014
created: 2014-10-06T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This part is the twin of the non-compatible biobrick BBa_K1486008 rendered compatible by mutating the two illegal restriction sites. However, all experiments were run on the aforementioned biobrick.
mutable notes: Site-directed mutagenesis was used to remove the restriction sites in the sequence.
mutable provenance: http://www.addgene.org/browse/article/8177/ which was then mutated by us.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_B0034
component: BBa_K1486004
component: BBa_I0500
component: BBa_K1486000
component: BBa_K1486045
component: BBa_K1486044
sequence annotation: BBa_I0500
sequence annotation: BBa_K1486000
sequence annotation: BBa_K1486004
sequence annotation: BBa_B0034
sequence annotation: BBa_K1486044
sequence annotation: BBa_K1486045
sequence: https://synbiohub.org/public/igem/BBa_K1486056_sequence/1

BBa_B0034
type: Component
persistent identity: component2398601
display id: component2398601
version: 1
top level: BBa_K1486056
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K1486004
type: Component
persistent identity: component2398606
display id: component2398606
version: 1
top level: BBa_K1486056
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486004/1

BBa_B0034
type: Component
persistent identity: component2398609
display id: component2398609
version: 1
top level: BBa_K1486056
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_I0500
type: Component
persistent identity: component2398599
display id: component2398599
version: 1
top level: BBa_K1486056
access: public
definition: https://synbiohub.org/public/igem/BBa_I0500/1

BBa_K1486000
type: Component
persistent identity: component2398613
display id: component2398613
version: 1
top level: BBa_K1486056
access: public
definition: BBa_K1486000

BBa_K1486004
type: Component
persistent identity: component2398614
display id: component2398614
version: 1
top level: BBa_K1486056
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486004/1

BBa_K1486045
type: Component
persistent identity: component2398615
display id: component2398615
version: 1
top level: BBa_K1486056
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486045/1

BBa_K1486000
type: Component
persistent identity: component2398605
display id: component2398605
version: 1
top level: BBa_K1486056
access: public
definition: BBa_K1486000

BBa_K1486044
type: Component
persistent identity: component2398607
display id: component2398607
version: 1
top level: BBa_K1486056
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486044/1

BBa_I0500
type: SequenceAnnotation
persistent identity: annotation2398599
display id: annotation2398599
version: 1
top level: BBa_K1486056
location: https://synbiohub.org/public/igem/BBa_K1486056/annotation2398599/range2398599/1
component: BBa_I0500

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type: Range
persistent identity: range2398599
display id: range2398599
version: 1
direction: direction/reverse
top level: BBa_K1486056
start: 1
end: 1210
orientation: inline

BBa_K1486000
type: SequenceAnnotation
persistent identity: annotation2398605
display id: annotation2398605
version: 1
top level: BBa_K1486056
location: https://synbiohub.org/public/igem/BBa_K1486056/annotation2398605/range2398605/1
component: BBa_K1486000

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type: Range
persistent identity: range2398605
display id: range2398605
version: 1
direction: direction/reverse
top level: BBa_K1486056
start: 1237
end: 1938
orientation: inline

BBa_K1486000
type: SequenceAnnotation
persistent identity: annotation2398613
display id: annotation2398613
version: 1
top level: BBa_K1486056
location: https://synbiohub.org/public/igem/BBa_K1486056/annotation2398613/range2398613/1
component: BBa_K1486000

https://synbiohub.org/public/igem/BBa_K1486056/annotation2398613/range2398613/1
type: Range
persistent identity: range2398613
display id: range2398613
version: 1
direction: direction/reverse
top level: BBa_K1486056
start: 2413
end: 3114
orientation: inline

BBa_K1486004
type: SequenceAnnotation
persistent identity: annotation2398606
display id: annotation2398606
version: 1
top level: BBa_K1486056
location: https://synbiohub.org/public/igem/BBa_K1486056/annotation2398606/range2398606/1
component: BBa_K1486004

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type: Range
persistent identity: range2398606
display id: range2398606
version: 1
direction: direction/reverse
top level: BBa_K1486056
start: 1947
end: 1976
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2398609
display id: annotation2398609
version: 1
top level: BBa_K1486056
location: https://synbiohub.org/public/igem/BBa_K1486056/annotation2398609/range2398609/1
component: BBa_B0034

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type: Range
persistent identity: range2398609
display id: range2398609
version: 1
direction: direction/reverse
top level: BBa_K1486056
start: 2395
end: 2406
orientation: inline

BBa_K1486044
type: SequenceAnnotation
persistent identity: annotation2398607
display id: annotation2398607
version: 1
top level: BBa_K1486056
location: https://synbiohub.org/public/igem/BBa_K1486056/annotation2398607/range2398607/1
component: BBa_K1486044

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type: Range
persistent identity: range2398607
display id: range2398607
version: 1
direction: direction/reverse
top level: BBa_K1486056
start: 1985
end: 2386
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2398601
display id: annotation2398601
version: 1
top level: BBa_K1486056
location: https://synbiohub.org/public/igem/BBa_K1486056/annotation2398601/range2398601/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1486056/annotation2398601/range2398601/1
type: Range
persistent identity: range2398601
display id: range2398601
version: 1
direction: direction/reverse
top level: BBa_K1486056
start: 1219
end: 1230
orientation: inline

BBa_K1486045
type: SequenceAnnotation
persistent identity: annotation2398615
display id: annotation2398615
version: 1
top level: BBa_K1486056
location: https://synbiohub.org/public/igem/BBa_K1486056/annotation2398615/range2398615/1
component: BBa_K1486045

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type: Range
persistent identity: range2398615
display id: range2398615
version: 1
direction: direction/reverse
top level: BBa_K1486056
start: 3161
end: 3742
orientation: inline

BBa_K1486004
type: SequenceAnnotation
persistent identity: annotation2398614
display id: annotation2398614
version: 1
top level: BBa_K1486056
location: https://synbiohub.org/public/igem/BBa_K1486056/annotation2398614/range2398614/1
component: BBa_K1486004

https://synbiohub.org/public/igem/BBa_K1486056/annotation2398614/range2398614/1
type: Range
persistent identity: range2398614
display id: range2398614
version: 1
direction: direction/reverse
top level: BBa_K1486056
start: 3123
end: 3152
orientation: inline

BBa_K1031442
type: ComponentDefinition
persistent identity: BBa K1031442
display id: BBa_K1031442
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: J23109-HcaR
creator: He Shuaixin
created: 2013-09-08T11:00:00Z
modified: 2015-05-08T01:08:47Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1338_
m user id: 0
owner id: 15843
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: 109-HcaR-TT
mutable notes: molecular cloning
mutable provenance: PCR genome E.coli
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: BBa_J23109
sequence annotation: HcaR
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K1031442_sequence/1

BBa_J23109
type: SequenceAnnotation
persistent identity: annotation2357456
display id: annotation2357456
version: 1
top level: BBa_K1031442
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1031442/annotation2357456/range2357456/1

https://synbiohub.org/public/igem/BBa_K1031442/annotation2357456/range2357456/1
type: Range
persistent identity: range2357456
display id: range2357456
version: 1
direction: direction/forward
top level: BBa_K1031442
start: 1
end: 35
orientation: inline

HcaR
type: SequenceAnnotation
persistent identity: annotation2357458
display id: annotation2357458
version: 1
top level: BBa_K1031442
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1031442/annotation2357458/range2357458/1

https://synbiohub.org/public/igem/BBa_K1031442/annotation2357458/range2357458/1
type: Range
persistent identity: range2357458
display id: range2357458
version: 1
direction: direction/forward
top level: BBa_K1031442
start: 62
end: 952
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2357457
display id: annotation2357457
version: 1
top level: BBa_K1031442
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1031442/annotation2357457/range2357457/1

https://synbiohub.org/public/igem/BBa_K1031442/annotation2357457/range2357457/1
type: Range
persistent identity: range2357457
display id: range2357457
version: 1
direction: direction/forward
top level: BBa_K1031442
start: 44
end: 55
orientation: inline

BBa_K1520024
type: ComponentDefinition
persistent identity: BBa K1520024
display id: BBa_K1520024
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Pcons2-rbs-lacI-Ter-Plac-rbs-luxI-Ter-Pcons2-rbs-luxR-Ter-Prlux-rbs-rfp-rbs-luxI-rbs-luxR-Ter
creator: yue wu
created: 2014-09-21T11:00:00Z
modified: 2015-05-08T01:10:49Z
discontinued: false
dominant: false
group u list: _1902_
m user id: 0
owner id: 21318
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Plasmid in positive feedback verification: Pcons2-rbs-lacI-Ter-Plac-rbs-luxI-Ter-Pcons2-rbs-luxR-Ter-Prlux-rbs-rfp-rbs-luxI-rbs-luxR-Ter It is a positive feedback plasmid using luxI and luxR as key to feedback. Introducing IPTG will produce rfp, luxI and luxR, and luxI together with luxR will work back to enhance production of rfp.
mutable notes: None
mutable provenance: Pcons2, lacI, Plac, luxI, luxR, Prlux, rfp, Ter
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1520013
component: BBa_K1520020
sequence annotation: BBa_K1520013
sequence annotation: BBa_K1520020
sequence: https://synbiohub.org/public/igem/BBa_K1520024_sequence/1

BBa_K1520013
type: Component
persistent identity: component2385264
display id: component2385264
version: 1
top level: BBa_K1520024
access: public
definition: BBa_K1520013

BBa_K1520020
type: Component
persistent identity: component2385250
display id: component2385250
version: 1
top level: BBa_K1520024
access: public
definition: BBa_K1520020

BBa_K1520013
type: SequenceAnnotation
persistent identity: annotation2385264
display id: annotation2385264
version: 1
top level: BBa_K1520024
location: https://synbiohub.org/public/igem/BBa_K1520024/annotation2385264/range2385264/1
component: BBa_K1520013

https://synbiohub.org/public/igem/BBa_K1520024/annotation2385264/range2385264/1
type: Range
persistent identity: range2385264
display id: range2385264
version: 1
direction: direction/reverse
top level: BBa_K1520024
start: 3361
end: 5768
orientation: inline

BBa_K1520020
type: SequenceAnnotation
persistent identity: annotation2385250
display id: annotation2385250
version: 1
top level: BBa_K1520024
location: https://synbiohub.org/public/igem/BBa_K1520024/annotation2385250/range2385250/1
component: BBa_K1520020

https://synbiohub.org/public/igem/BBa_K1520024/annotation2385250/range2385250/1
type: Range
persistent identity: range2385250
display id: range2385250
version: 1
direction: direction/reverse
top level: BBa_K1520024
start: 1
end: 3352
orientation: inline

BBa_K1031019
type: ComponentDefinition
persistent identity: BBa K1031019
display id: BBa_K1031019
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: NahR-Psal-B0034-RiboJ-φR73δ
creator: He Shuaixin
created: 2013-10-21T11:00:00Z
modified: 2015-05-08T01:08:47Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1338_
m user id: 0
owner id: 15843
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: NahR-Psal-φR73Δ
mutable notes: molecular cloning
mutable provenance: φR73; Pseudomonas. putida
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: NahR
sequence annotation: Pr
sequence annotation: Psal
sequence annotation: phiR73delta
sequence: https://synbiohub.org/public/igem/BBa_K1031019_sequence/1

NahR
type: SequenceAnnotation
persistent identity: annotation2370291
display id: annotation2370291
version: 1
top level: BBa_K1031019
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1031019/annotation2370291/range2370291/1

https://synbiohub.org/public/igem/BBa_K1031019/annotation2370291/range2370291/1
type: Range
persistent identity: range2370291
display id: range2370291
version: 1
direction: direction/reverse
top level: BBa_K1031019
start: 211
end: 1113
orientation: inline

Pr
type: SequenceAnnotation
persistent identity: annotation2370292
display id: annotation2370292
version: 1
top level: BBa_K1031019
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1031019/annotation2370292/range2370292/1

https://synbiohub.org/public/igem/BBa_K1031019/annotation2370292/range2370292/1
type: Range
persistent identity: range2370292
display id: range2370292
version: 1
direction: direction/reverse
top level: BBa_K1031019
start: 1114
end: 1203
orientation: inline

Psal
type: SequenceAnnotation
persistent identity: annotation2370293
display id: annotation2370293
version: 1
top level: BBa_K1031019
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1031019/annotation2370293/range2370293/1

https://synbiohub.org/public/igem/BBa_K1031019/annotation2370293/range2370293/1
type: Range
persistent identity: range2370293
display id: range2370293
version: 1
direction: direction/forward
top level: BBa_K1031019
start: 1192
end: 1260
orientation: inline

phiR73delta
type: SequenceAnnotation
persistent identity: annotation2370294
display id: annotation2370294
version: 1
top level: BBa_K1031019
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1031019/annotation2370294/range2370294/1

https://synbiohub.org/public/igem/BBa_K1031019/annotation2370294/range2370294/1
type: Range
persistent identity: range2370294
display id: range2370294
version: 1
direction: direction/forward
top level: BBa_K1031019
start: 1370
end: 1615
orientation: inline

BBa_K1520501
type: ComponentDefinition
persistent identity: BBa K1520501
display id: BBa_K1520501
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Rbs-luxR(scarless)
creator: yue wu
created: 2014-09-21T11:00:00Z
modified: 2015-05-08T01:10:49Z
discontinued: false
dominant: false
group u list: _1902_
m user id: 0
owner id: 21318
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Rbs-luxR(scarless)
mutable notes: None
mutable provenance: None
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
sequence annotation: luxR
sequence annotation: rbs
sequence: https://synbiohub.org/public/igem/BBa_K1520501_sequence/1

luxR
type: SequenceAnnotation
persistent identity: annotation2384862
display id: annotation2384862
version: 1
top level: BBa_K1520501
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1520501/annotation2384862/range2384862/1

https://synbiohub.org/public/igem/BBa_K1520501/annotation2384862/range2384862/1
type: Range
persistent identity: range2384862
display id: range2384862
version: 1
direction: direction/forward
top level: BBa_K1520501
start: 19
end: 799
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation2384861
display id: annotation2384861
version: 1
top level: BBa_K1520501
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1520501/annotation2384861/range2384861/1

https://synbiohub.org/public/igem/BBa_K1520501/annotation2384861/range2384861/1
type: Range
persistent identity: range2384861
display id: range2384861
version: 1
direction: direction/forward
top level: BBa_K1520501
start: 1
end: 18
orientation: inline

BBa_K1486014
type: ComponentDefinition
persistent identity: BBa K1486014
display id: BBa_K1486014
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: IFP[1]-CpxR under pBAD
creator: iGem EPFL 2014
created: 2014-08-31T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part has a fused protein downstream of an arabinose-inducible promoter: IFP[1]-CpxR. Biobrick BBa_K1486015 has IFP[2]-CpxR with an arabinose promoter. This construct was made in both orientations: BBa_K1486012 is CpxR-IFP[1] (with an arabinose promoter).
mutable notes: Both orientations were made, as well as for IFP[2] so all dimerizations could be tested (see main page for biobrick numbers). Flexible linkers were used to fuse both proteins, and an RBS was put between the promoter and the cds.
mutable provenance: to edit
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_I0500
component: BBa_K1486000
component: BBa_B0034
component: BBa_K1486004
component: BBa_K1486006
sequence annotation: BBa_K1486006
sequence annotation: BBa_K1486004
sequence annotation: BBa_I0500
sequence annotation: BBa_B0034
sequence annotation: BBa_K1486000
sequence: https://synbiohub.org/public/igem/BBa_K1486014_sequence/1

BBa_I0500
type: Component
persistent identity: component2424736
display id: component2424736
version: 1
top level: BBa_K1486014
access: public
definition: https://synbiohub.org/public/igem/BBa_I0500/1

BBa_K1486000
type: Component
persistent identity: component2424747
display id: component2424747
version: 1
top level: BBa_K1486014
access: public
definition: BBa_K1486000

BBa_B0034
type: Component
persistent identity: component2424738
display id: component2424738
version: 1
top level: BBa_K1486014
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K1486004
type: Component
persistent identity: component2424743
display id: component2424743
version: 1
top level: BBa_K1486014
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486004/1

BBa_K1486006
type: Component
persistent identity: component2424742
display id: component2424742
version: 1
top level: BBa_K1486014
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486006/1

BBa_K1486006
type: SequenceAnnotation
persistent identity: annotation2424742
display id: annotation2424742
version: 1
top level: BBa_K1486014
location: https://synbiohub.org/public/igem/BBa_K1486014/annotation2424742/range2424742/1
component: BBa_K1486006

https://synbiohub.org/public/igem/BBa_K1486014/annotation2424742/range2424742/1
type: Range
persistent identity: range2424742
display id: range2424742
version: 1
direction: direction/reverse
top level: BBa_K1486014
start: 1223
end: 1627
orientation: inline

BBa_K1486004
type: SequenceAnnotation
persistent identity: annotation2424743
display id: annotation2424743
version: 1
top level: BBa_K1486014
location: https://synbiohub.org/public/igem/BBa_K1486014/annotation2424743/range2424743/1
component: BBa_K1486004

https://synbiohub.org/public/igem/BBa_K1486014/annotation2424743/range2424743/1
type: Range
persistent identity: range2424743
display id: range2424743
version: 1
direction: direction/reverse
top level: BBa_K1486014
start: 1628
end: 1657
orientation: inline

BBa_I0500
type: SequenceAnnotation
persistent identity: annotation2424736
display id: annotation2424736
version: 1
top level: BBa_K1486014
location: https://synbiohub.org/public/igem/BBa_K1486014/annotation2424736/range2424736/1
component: BBa_I0500

https://synbiohub.org/public/igem/BBa_K1486014/annotation2424736/range2424736/1
type: Range
persistent identity: range2424736
display id: range2424736
version: 1
direction: direction/reverse
top level: BBa_K1486014
start: 1
end: 1210
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2424738
display id: annotation2424738
version: 1
top level: BBa_K1486014
location: https://synbiohub.org/public/igem/BBa_K1486014/annotation2424738/range2424738/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1486014/annotation2424738/range2424738/1
type: Range
persistent identity: range2424738
display id: range2424738
version: 1
direction: direction/reverse
top level: BBa_K1486014
start: 1211
end: 1222
orientation: inline

BBa_K1486000
type: SequenceAnnotation
persistent identity: annotation2424747
display id: annotation2424747
version: 1
top level: BBa_K1486014
location: https://synbiohub.org/public/igem/BBa_K1486014/annotation2424747/range2424747/1
component: BBa_K1486000

https://synbiohub.org/public/igem/BBa_K1486014/annotation2424747/range2424747/1
type: Range
persistent identity: range2424747
display id: range2424747
version: 1
direction: direction/reverse
top level: BBa_K1486014
start: 1658
end: 2359
orientation: inline

BBa_K389011
type: ComponentDefinition
persistent identity: BBa K389011
display id: BBa_K389011
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: VirA screening device
creator: Jonas Aretz
created: 2010-08-09T11:00:00Z
modified: 2015-05-08T01:12:19Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _517_
m user id: 0
owner id: 6670
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This part contains: - a mutated ''virG'' response regulator from the virA/G receptor system from ''Agrobacterium tumefaciens'' which works in ''Escherichia coli'' without an ''rpoA'' gene from ''A. tumefaciens'' - a kanamycin resistance under the control of a ''vir'' promotor This device is used to screen ''virA'' receptor mutants. The ''virA'' receptor is mutated in the BBa_K389010 BioBrick. The better the receptor detects a substance, the more ''virG'' is phosphorylated and the stronger the kanamycin resistance from this BioBrick is expressed. The BioBricks BBa_K389010 and BBa_K389011 have to be used together in one cell.
mutable notes: medium strong constitutive promotor, terminator to keep basal expression of kanamycin resistance low
mutable provenance: synthetic ''virG'' gene, ''vir'' promotor from ''A. tumefaciens'' C58, partsregistry
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_J23110
component: BBa_K389003
component: BBa_K389002
component: BBa_K389005
component: BBa_B0010
sequence annotation: BBa_B0010
sequence annotation: BBa_J23110
sequence annotation: BBa_K389005
sequence annotation: BBa_K389002
sequence annotation: BBa_K389003
sequence: https://synbiohub.org/public/igem/BBa_K389011_sequence/1

BBa_J23110
type: Component
persistent identity: component2078366
display id: component2078366
version: 1
top level: BBa_K389011
access: public
definition: https://synbiohub.org/public/igem/BBa_J23110/1

BBa_K389003
type: Component
persistent identity: component2078379
display id: component2078379
version: 1
top level: BBa_K389011
access: public
definition: https://synbiohub.org/public/igem/BBa_K389003/1

BBa_K389002
type: Component
persistent identity: component2078370
display id: component2078370
version: 1
top level: BBa_K389011
access: public
definition: virG

BBa_K389005
type: Component
persistent identity: component2078382
display id: component2078382
version: 1
top level: BBa_K389011
access: public
definition: https://synbiohub.org/public/igem/BBa_K389005/1

BBa_B0010
type: Component
persistent identity: component2078371
display id: component2078371
version: 1
top level: BBa_K389011
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0010
type: Component
persistent identity: component2078373
display id: component2078373
version: 1
top level: BBa_K389011
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2078371
display id: annotation2078371
version: 1
top level: BBa_K389011
location: https://synbiohub.org/public/igem/BBa_K389011/annotation2078371/range2078371/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K389011/annotation2078371/range2078371/1
type: Range
persistent identity: range2078371
display id: range2078371
version: 1
direction: direction/forward
top level: BBa_K389011
start: 792
end: 871
orientation: inline

BBa_J23110
type: SequenceAnnotation
persistent identity: annotation2078366
display id: annotation2078366
version: 1
top level: BBa_K389011
location: https://synbiohub.org/public/igem/BBa_K389011/annotation2078366/range2078366/1
component: BBa_J23110

https://synbiohub.org/public/igem/BBa_K389011/annotation2078366/range2078366/1
type: Range
persistent identity: range2078366
display id: range2078366
version: 1
direction: direction/reverse
top level: BBa_K389011
start: 1
end: 35
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2078373
display id: annotation2078373
version: 1
top level: BBa_K389011
location: https://synbiohub.org/public/igem/BBa_K389011/annotation2078373/range2078373/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K389011/annotation2078373/range2078373/1
type: Range
persistent identity: range2078373
display id: range2078373
version: 1
direction: direction/forward
top level: BBa_K389011
start: 880
end: 959
orientation: inline

BBa_K389005
type: SequenceAnnotation
persistent identity: annotation2078382
display id: annotation2078382
version: 1
top level: BBa_K389011
location: https://synbiohub.org/public/igem/BBa_K389011/annotation2078382/range2078382/1
component: BBa_K389005

https://synbiohub.org/public/igem/BBa_K389011/annotation2078382/range2078382/1
type: Range
persistent identity: range2078382
display id: range2078382
version: 1
direction: direction/reverse
top level: BBa_K389011
start: 1132
end: 1950
orientation: inline

BBa_K389002
type: SequenceAnnotation
persistent identity: annotation2078370
display id: annotation2078370
version: 1
top level: BBa_K389011
location: https://synbiohub.org/public/igem/BBa_K389011/annotation2078370/range2078370/1
component: BBa_K389002

https://synbiohub.org/public/igem/BBa_K389011/annotation2078370/range2078370/1
type: Range
persistent identity: range2078370
display id: range2078370
version: 1
direction: direction/reverse
top level: BBa_K389011
start: 44
end: 783
orientation: inline

BBa_K389003
type: SequenceAnnotation
persistent identity: annotation2078379
display id: annotation2078379
version: 1
top level: BBa_K389011
location: https://synbiohub.org/public/igem/BBa_K389011/annotation2078379/range2078379/1
component: BBa_K389003

https://synbiohub.org/public/igem/BBa_K389011/annotation2078379/range2078379/1
type: Range
persistent identity: range2078379
display id: range2078379
version: 1
direction: direction/reverse
top level: BBa_K389011
start: 968
end: 1125
orientation: inline

rcsA
type: ComponentDefinition
persistent identity: BBa K137113
display id: BBa_K137113
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: rcsA
creator: Fei Chen
created: 2008-09-23T11:00:00Z
modified: 2015-05-08T01:10:11Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _187_
m user id: 0
owner id: 2988
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: rcsA is a gene responsible for regulating capsule synthesis. It has also been shown to induce lambda lysogens into the lytic cycle.
mutable notes: N/A
mutable provenance: DH10B genomic DNA.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K137113_sequence/1

lasr
type: ComponentDefinition
persistent identity: BBa C0079
display id: BBa_C0079
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: lasR activator from P. aeruginosa PAO1(+LVA)
creator: Alvin Carter Powers (Fighting Darwins)
created: 2004-01-27T12:00:00Z
modified: 2015-08-31T04:07:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: coding region for lasR protein, which accepts chemical signal AI-1 (made by lasI protein)
mutable notes: Mutated 480 from g to a to remove PstI site
mutable provenance: Genbank: NC_002516 (www.ncbi.nlm.nih.gov)
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: LVA
sequence annotation: Help:Barcodes
sequence annotation: lasR
sequence annotation: G
sequence: https://synbiohub.org/public/igem/BBa_C0079_sequence/1

LVA
type: SequenceAnnotation
persistent identity: annotation307948
display id: annotation307948
version: 1
top level: lasr
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_C0079/annotation307948/range307948/1

https://synbiohub.org/public/igem/BBa_C0079/annotation307948/range307948/1
type: Range
persistent identity: range307948
display id: range307948
version: 1
direction: direction/forward
top level: lasr
start: 718
end: 750
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213999
display id: annotation2213999
version: 1
top level: lasr
role: SO:0000807
role: feature/barcode
location: https://synbiohub.org/public/igem/BBa_C0079/annotation2213999/range2213999/1

https://synbiohub.org/public/igem/BBa_C0079/annotation2213999/range2213999/1
type: Range
persistent identity: range2213999
display id: range2213999
version: 1
direction: direction/forward
top level: lasr
start: 757
end: 781
orientation: inline

lasR
type: SequenceAnnotation
persistent identity: annotation307935
display id: annotation307935
version: 1
top level: lasr
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_C0079/annotation307935/range307935/1

https://synbiohub.org/public/igem/BBa_C0079/annotation307935/range307935/1
type: Range
persistent identity: range307935
display id: range307935
version: 1
direction: direction/forward
top level: lasr
start: 1
end: 717
orientation: inline

G
type: SequenceAnnotation
persistent identity: annotation308006
display id: annotation308006
version: 1
top level: lasr
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_C0079/annotation308006/range308006/1

https://synbiohub.org/public/igem/BBa_C0079/annotation308006/range308006/1
type: Range
persistent identity: range308006
display id: range308006
version: 1
direction: direction/forward
top level: lasr
start: 480
end: 480
orientation: inline

BBa_K1486015
type: ComponentDefinition
persistent identity: BBa K1486015
display id: BBa_K1486015
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: IFP[2]-CpxR under pBAD
creator: EPFL iGem team 2014
created: 2014-09-09T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part has a fused protein downstream of an arabinose-inducible promoter: IFP[2]-CpxR. Biobrick BBa_K1486014 has IFP[1]-CpxR with an arabinose promoter. This construct was made in both orientations: BBa_K1486013 is CpxR-IFP[2] (with an arabinose promoter).
mutable notes: Both orientations were made, as well as for IFP[1] so all dimerizations could be tested (see main page for biobrick numbers). Flexible linkers were used to fuse both proteins, and an RBS was put before each one of them.
mutable provenance: To edit.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K1486007
component: BBa_K1486004
component: BBa_I0500
component: BBa_B0034
component: BBa_K1486000
sequence annotation: BBa_K1486004
sequence annotation: BBa_K1486000
sequence annotation: BBa_I0500
sequence annotation: BBa_B0034
sequence annotation: BBa_K1486007
sequence: https://synbiohub.org/public/igem/BBa_K1486015_sequence/1

BBa_K1486007
type: Component
persistent identity: component2382854
display id: component2382854
version: 1
top level: BBa_K1486015
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486007/1

BBa_K1486004
type: Component
persistent identity: component2382855
display id: component2382855
version: 1
top level: BBa_K1486015
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486004/1

BBa_I0500
type: Component
persistent identity: component2382848
display id: component2382848
version: 1
top level: BBa_K1486015
access: public
definition: https://synbiohub.org/public/igem/BBa_I0500/1

BBa_B0034
type: Component
persistent identity: component2382850
display id: component2382850
version: 1
top level: BBa_K1486015
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K1486000
type: Component
persistent identity: component2382859
display id: component2382859
version: 1
top level: BBa_K1486015
access: public
definition: BBa_K1486000

BBa_K1486004
type: SequenceAnnotation
persistent identity: annotation2382855
display id: annotation2382855
version: 1
top level: BBa_K1486015
location: https://synbiohub.org/public/igem/BBa_K1486015/annotation2382855/range2382855/1
component: BBa_K1486004

https://synbiohub.org/public/igem/BBa_K1486015/annotation2382855/range2382855/1
type: Range
persistent identity: range2382855
display id: range2382855
version: 1
direction: direction/reverse
top level: BBa_K1486015
start: 1824
end: 1853
orientation: inline

BBa_K1486000
type: SequenceAnnotation
persistent identity: annotation2382859
display id: annotation2382859
version: 1
top level: BBa_K1486015
location: https://synbiohub.org/public/igem/BBa_K1486015/annotation2382859/range2382859/1
component: BBa_K1486000

https://synbiohub.org/public/igem/BBa_K1486015/annotation2382859/range2382859/1
type: Range
persistent identity: range2382859
display id: range2382859
version: 1
direction: direction/reverse
top level: BBa_K1486015
start: 1860
end: 2561
orientation: inline

BBa_I0500
type: SequenceAnnotation
persistent identity: annotation2382848
display id: annotation2382848
version: 1
top level: BBa_K1486015
location: https://synbiohub.org/public/igem/BBa_K1486015/annotation2382848/range2382848/1
component: BBa_I0500

https://synbiohub.org/public/igem/BBa_K1486015/annotation2382848/range2382848/1
type: Range
persistent identity: range2382848
display id: range2382848
version: 1
direction: direction/reverse
top level: BBa_K1486015
start: 1
end: 1210
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2382850
display id: annotation2382850
version: 1
top level: BBa_K1486015
location: https://synbiohub.org/public/igem/BBa_K1486015/annotation2382850/range2382850/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1486015/annotation2382850/range2382850/1
type: Range
persistent identity: range2382850
display id: range2382850
version: 1
direction: direction/reverse
top level: BBa_K1486015
start: 1219
end: 1230
orientation: inline

BBa_K1486007
type: SequenceAnnotation
persistent identity: annotation2382854
display id: annotation2382854
version: 1
top level: BBa_K1486015
location: https://synbiohub.org/public/igem/BBa_K1486015/annotation2382854/range2382854/1
component: BBa_K1486007

https://synbiohub.org/public/igem/BBa_K1486015/annotation2382854/range2382854/1
type: Range
persistent identity: range2382854
display id: range2382854
version: 1
direction: direction/reverse
top level: BBa_K1486015
start: 1237
end: 1815
orientation: inline

BBa_K1520012
type: ComponentDefinition
persistent identity: BBa K1520012
display id: BBa_K1520012
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Prlux-rbs-rfp-rbs-luxR-Ter
creator: yue wu
created: 2014-09-21T11:00:00Z
modified: 2015-05-08T01:10:49Z
discontinued: false
dominant: false
group u list: _1902_
m user id: 0
owner id: 21318
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Prlux-rbs-rfp-rbs-luxR-Ter. It produces rfp and luxR when there is enough rfp and luxR.
mutable notes: None
mutable provenance: Prlux, rfp, luxR, Ter
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1520029
component: BBa_K1520501
component: BBa_K1520500
component: BBa_R0062
sequence annotation: BBa_K1520501
sequence annotation: BBa_R0062
sequence annotation: BBa_K1520500
sequence annotation: BBa_K1520029
sequence: https://synbiohub.org/public/igem/BBa_K1520012_sequence/1

BBa_K1520029
type: Component
persistent identity: component2384977
display id: component2384977
version: 1
top level: BBa_K1520012
access: public
definition: https://synbiohub.org/public/igem/BBa_K1520029/1

BBa_K1520501
type: Component
persistent identity: component2384976
display id: component2384976
version: 1
top level: BBa_K1520012
access: public
definition: BBa_K1520501

BBa_K1520500
type: Component
persistent identity: component2384973
display id: component2384973
version: 1
top level: BBa_K1520012
access: public
definition: https://synbiohub.org/public/igem/BBa_K1520500/1

BBa_R0062
type: Component
persistent identity: component2384968
display id: component2384968
version: 1
top level: BBa_K1520012
access: public
definition: https://synbiohub.org/public/igem/BBa_R0062/1

BBa_K1520501
type: SequenceAnnotation
persistent identity: annotation2384976
display id: annotation2384976
version: 1
top level: BBa_K1520012
location: https://synbiohub.org/public/igem/BBa_K1520012/annotation2384976/range2384976/1
component: BBa_K1520501

https://synbiohub.org/public/igem/BBa_K1520012/annotation2384976/range2384976/1
type: Range
persistent identity: range2384976
display id: range2384976
version: 1
direction: direction/reverse
top level: BBa_K1520012
start: 804
end: 1602
orientation: inline

BBa_R0062
type: SequenceAnnotation
persistent identity: annotation2384968
display id: annotation2384968
version: 1
top level: BBa_K1520012
location: https://synbiohub.org/public/igem/BBa_K1520012/annotation2384968/range2384968/1
component: BBa_R0062

https://synbiohub.org/public/igem/BBa_K1520012/annotation2384968/range2384968/1
type: Range
persistent identity: range2384968
display id: range2384968
version: 1
direction: direction/forward
top level: BBa_K1520012
start: 1
end: 55
orientation: inline

BBa_K1520500
type: SequenceAnnotation
persistent identity: annotation2384973
display id: annotation2384973
version: 1
top level: BBa_K1520012
location: https://synbiohub.org/public/igem/BBa_K1520012/annotation2384973/range2384973/1
component: BBa_K1520500

https://synbiohub.org/public/igem/BBa_K1520012/annotation2384973/range2384973/1
type: Range
persistent identity: range2384973
display id: range2384973
version: 1
direction: direction/reverse
top level: BBa_K1520012
start: 64
end: 795
orientation: inline

BBa_K1520029
type: SequenceAnnotation
persistent identity: annotation2384977
display id: annotation2384977
version: 1
top level: BBa_K1520012
location: https://synbiohub.org/public/igem/BBa_K1520012/annotation2384977/range2384977/1
component: BBa_K1520029

https://synbiohub.org/public/igem/BBa_K1520012/annotation2384977/range2384977/1
type: Range
persistent identity: range2384977
display id: range2384977
version: 1
direction: direction/reverse
top level: BBa_K1520012
start: 1611
end: 1739
orientation: inline

BBa_K1031100
type: ComponentDefinition
persistent identity: BBa K1031100
display id: BBa_K1031100
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CapR-Terminator
creator: He Shuaixin
created: 2013-09-08T11:00:00Z
modified: 2015-05-08T01:08:47Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1338_
m user id: 0
owner id: 15843
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: CapR-TT
mutable notes: Pseudomonas putida
mutable provenance: synthesis
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: CapR
sequence annotation: B0015
sequence: https://synbiohub.org/public/igem/BBa_K1031100_sequence/1

CapR
type: SequenceAnnotation
persistent identity: annotation2361063
display id: annotation2361063
version: 1
top level: BBa_K1031100
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1031100/annotation2361063/range2361063/1

https://synbiohub.org/public/igem/BBa_K1031100/annotation2361063/range2361063/1
type: Range
persistent identity: range2361063
display id: range2361063
version: 1
direction: direction/forward
top level: BBa_K1031100
start: 1
end: 1692
orientation: inline

B0015
type: SequenceAnnotation
persistent identity: annotation2361122
display id: annotation2361122
version: 1
top level: BBa_K1031100
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1031100/annotation2361122/range2361122/1

https://synbiohub.org/public/igem/BBa_K1031100/annotation2361122/range2361122/1
type: Range
persistent identity: range2361122
display id: range2361122
version: 1
direction: direction/forward
top level: BBa_K1031100
start: 1699
end: 1827
orientation: inline

BBa_K1031017
type: ComponentDefinition
persistent identity: BBa K1031017
display id: BBa_K1031017
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: J23114-B0034-RiboJ-φR73δ
creator: He Shuaixin
created: 2013-10-21T11:00:00Z
modified: 2015-05-08T01:08:47Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1338_
m user id: 0
owner id: 15843
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: J23114-φR73Δ
mutable notes: molecular cloning
mutable provenance: φR73
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: B0034
sequence annotation: J23114
sequence annotation: phiR73delta
sequence: https://synbiohub.org/public/igem/BBa_K1031017_sequence/1

B0034
type: SequenceAnnotation
persistent identity: annotation2370287
display id: annotation2370287
version: 1
top level: BBa_K1031017
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1031017/annotation2370287/range2370287/1

https://synbiohub.org/public/igem/BBa_K1031017/annotation2370287/range2370287/1
type: Range
persistent identity: range2370287
display id: range2370287
version: 1
direction: direction/forward
top level: BBa_K1031017
start: 118
end: 129
orientation: inline

J23114
type: SequenceAnnotation
persistent identity: annotation2370286
display id: annotation2370286
version: 1
top level: BBa_K1031017
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1031017/annotation2370286/range2370286/1

https://synbiohub.org/public/igem/BBa_K1031017/annotation2370286/range2370286/1
type: Range
persistent identity: range2370286
display id: range2370286
version: 1
direction: direction/forward
top level: BBa_K1031017
start: 1
end: 35
orientation: inline

phiR73delta
type: SequenceAnnotation
persistent identity: annotation2370288
display id: annotation2370288
version: 1
top level: BBa_K1031017
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1031017/annotation2370288/range2370288/1

https://synbiohub.org/public/igem/BBa_K1031017/annotation2370288/range2370288/1
type: Range
persistent identity: range2370288
display id: range2370288
version: 1
direction: direction/forward
top level: BBa_K1031017
start: 136
end: 381
orientation: inline

virG
type: ComponentDefinition
persistent identity: BBa K389002
display id: BBa_K389002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Mutated virG
creator: Jonas Aretz
created: 2010-08-09T11:00:00Z
modified: 2015-05-08T01:12:18Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _517_
m user id: 0
owner id: 6670
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This is the ''virG'' response regulator from the virA/G receptor system. The virA/G receptor system is used by ''Agrobacterium tumefaciens'' to detect phenolic substances secreted by plants. In presence of these substances, ''virG'' is activated by the ''virA'' receptor and induces the transcription of genes under the control of a ''vir'' promotor. Normally, ''virG'' needs the ''rpoA'' RNA polymerase subunit from ''A. tumefaciens'' to work in ''Escherichia coli'' but this ''virG'' BioBrick is mutated, so works with the ''rpoA'' subunit from ''E. coli''.
mutable notes: synthesized by Mr. Gene, so optimated codon usage, removal of illegal restriction sites
mutable provenance: synthetic
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: RBS
sequence annotation: Stop codon
sequence annotation: virG
sequence: https://synbiohub.org/public/igem/BBa_K389002_sequence/1

RBS
type: SequenceAnnotation
persistent identity: annotation2078360
display id: annotation2078360
version: 1
top level: virG
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K389002/annotation2078360/range2078360/1

https://synbiohub.org/public/igem/BBa_K389002/annotation2078360/range2078360/1
type: Range
persistent identity: range2078360
display id: range2078360
version: 1
direction: direction/forward
top level: virG
start: 4
end: 8
orientation: inline

Stop codon
type: SequenceAnnotation
persistent identity: annotation2078362
display id: annotation2078362
version: 1
top level: virG
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K389002/annotation2078362/range2078362/1

https://synbiohub.org/public/igem/BBa_K389002/annotation2078362/range2078362/1
type: Range
persistent identity: range2078362
display id: range2078362
version: 1
direction: direction/forward
top level: virG
start: 738
end: 740
orientation: inline

virG
type: SequenceAnnotation
persistent identity: annotation2078361
display id: annotation2078361
version: 1
top level: virG
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K389002/annotation2078361/range2078361/1

https://synbiohub.org/public/igem/BBa_K389002/annotation2078361/range2078361/1
type: Range
persistent identity: range2078361
display id: range2078361
version: 1
direction: direction/forward
top level: virG
start: 12
end: 737
orientation: inline

LuxO D47E
type: ComponentDefinition
persistent identity: BBa K131022
display id: BBa_K131022
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LuxO D47E, Vibrio harveyi
creator: Thane Kubik
created: 2008-10-27T12:00:00Z
modified: 2015-05-08T01:09:47Z
discontinued: false
dominant: true
experience: experience/None
group u list: _259_
m user id: 0
owner id: 3190
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: LuxO D47E, Vibrio harveyi is a constitutively active mutant of LuxO
mutable notes: The plasmid containing LuxOD47E (pJAF822) was obtained from Bassler lab (Freeman and Bassler, 1999). Jeremy A. Freeman and Bonnie L. Bassler. A genetic analysis of the function of LuxO, a two-component response regulator involved in quorum sensing in Vibrio harveyi. 1999a. Molecular Microbiology. 31(2), 665-677.
mutable provenance: >gi|156972381:c2983472-2982069 Vibrio harveyi ATCC BAA-1116 chromosome I, complete sequence Nucleotide number 141 has been mutated from T to G, thus bases 139-141 have been changed from GAT (Aspartic acid, D) to GAG (Glutamine, E). Jeremy A. Freeman and Bonnie L. Bassler. A genetic analysis of the function of LuxO, a two-component response regulator involved in quorum sensing in Vibrio harveyi. 1999a. Molecular Microbiology. 31(2), 665-677.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: D47E
sequence annotation: Start
sequence annotation: Stop
sequence: https://synbiohub.org/public/igem/BBa_K131022_sequence/1

D47E
type: SequenceAnnotation
persistent identity: annotation1990654
display id: annotation1990654
version: 1
top level: LuxO D47E
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K131022/annotation1990654/range1990654/1

https://synbiohub.org/public/igem/BBa_K131022/annotation1990654/range1990654/1
type: Range
persistent identity: range1990654
display id: range1990654
version: 1
direction: direction/forward
top level: LuxO D47E
start: 139
end: 141
orientation: inline

Start
type: SequenceAnnotation
persistent identity: annotation1990655
display id: annotation1990655
version: 1
top level: LuxO D47E
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K131022/annotation1990655/range1990655/1

https://synbiohub.org/public/igem/BBa_K131022/annotation1990655/range1990655/1
type: Range
persistent identity: range1990655
display id: range1990655
version: 1
direction: direction/forward
top level: LuxO D47E
start: 1
end: 3
orientation: inline

Stop
type: SequenceAnnotation
persistent identity: annotation1990656
display id: annotation1990656
version: 1
top level: LuxO D47E
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K131022/annotation1990656/range1990656/1

https://synbiohub.org/public/igem/BBa_K131022/annotation1990656/range1990656/1
type: Range
persistent identity: range1990656
display id: range1990656
version: 1
direction: direction/forward
top level: LuxO D47E
start: 1360
end: 1362
orientation: inline

appY
type: ComponentDefinition
persistent identity: BBa K118001
display id: BBa_K118001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: appY coding sequence encoding a DNA-binding transcriptional activator
creator: Andrew Hall
created: 2008-07-21T11:00:00Z
modified: 2015-05-08T01:09:36Z
discontinued: false
dominant: false
experience: experience/None
group u list: _192_
m user id: 0
owner id: 3282
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: This is the coding sequence of appY from Escherichia coli JM109. It encodes a transcriptional regulator related to anaerobic energy metabolism. Overexpression of the gene has been reported to result in enhanced lycopene production by E. coli (Kang et al., 2005).
mutable notes: No special considerations.
mutable provenance: Escherichia coli JM109 genomic DNA.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: appY coding sequence
sequence: https://synbiohub.org/public/igem/BBa_K118001_sequence/1

appY coding sequence
type: SequenceAnnotation
persistent identity: annotation1995700
display id: annotation1995700
version: 1
top level: appY
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K118001/annotation1995700/range1995700/1

https://synbiohub.org/public/igem/BBa_K118001/annotation1995700/range1995700/1
type: Range
persistent identity: range1995700
display id: range1995700
version: 1
direction: direction/forward
top level: appY
start: 1
end: 750
orientation: inline

BBa_K1031112
type: ComponentDefinition
persistent identity: BBa K1031112
display id: BBa_K1031112
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: J23113-CapR-Terminator
creator: He Shuaixin
created: 2013-09-08T11:00:00Z
modified: 2015-05-08T01:08:47Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1338_
m user id: 0
owner id: 15843
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: 113-CapR-TT
mutable notes: molecular cloning
mutable provenance: synthesis Pseudomonas putida
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: J23113
sequence annotation: CapR
sequence annotation: B0015
sequence: https://synbiohub.org/public/igem/BBa_K1031112_sequence/1

J23113
type: SequenceAnnotation
persistent identity: annotation2361448
display id: annotation2361448
version: 1
top level: BBa_K1031112
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1031112/annotation2361448/range2361448/1

https://synbiohub.org/public/igem/BBa_K1031112/annotation2361448/range2361448/1
type: Range
persistent identity: range2361448
display id: range2361448
version: 1
direction: direction/forward
top level: BBa_K1031112
start: 1
end: 35
orientation: inline

CapR
type: SequenceAnnotation
persistent identity: annotation2361507
display id: annotation2361507
version: 1
top level: BBa_K1031112
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1031112/annotation2361507/range2361507/1

https://synbiohub.org/public/igem/BBa_K1031112/annotation2361507/range2361507/1
type: Range
persistent identity: range2361507
display id: range2361507
version: 1
direction: direction/forward
top level: BBa_K1031112
start: 62
end: 1753
orientation: inline

B0015
type: SequenceAnnotation
persistent identity: annotation2361508
display id: annotation2361508
version: 1
top level: BBa_K1031112
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1031112/annotation2361508/range2361508/1

https://synbiohub.org/public/igem/BBa_K1031112/annotation2361508/range2361508/1
type: Range
persistent identity: range2361508
display id: range2361508
version: 1
direction: direction/forward
top level: BBa_K1031112
start: 1760
end: 1888
orientation: inline

BBa_K294205
type: ComponentDefinition
persistent identity: BBa K294205
display id: BBa_K294205
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: This is a coding sequence of heat shock protein from E.coli
creator: Koji Sode
created: 2009-06-26T11:00:00Z
modified: 2015-05-08T01:11:49Z
discontinued: false
dominant: false
group u list: _397_
m user id: 0
owner id: 5334
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: this is a coding sequence of heat shock protein from E.coli. It regulate heat shock proteins .....
mutable notes: We chose this proiten because ...
mutable provenance: HSP15 derived from genome E.coli K12 http://biocyc.org/ECOLI/NEW-IMAGE?type=ENZYME&object=G7743-MONOMER
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: hslR
sequence annotation: bidding domain
sequence: https://synbiohub.org/public/igem/BBa_K294205_sequence/1

hslR
type: SequenceAnnotation
persistent identity: annotation2006747
display id: annotation2006747
version: 1
top level: BBa_K294205
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K294205/annotation2006747/range2006747/1

https://synbiohub.org/public/igem/BBa_K294205/annotation2006747/range2006747/1
type: Range
persistent identity: range2006747
display id: range2006747
version: 1
direction: direction/forward
top level: BBa_K294205
start: 1
end: 402
orientation: inline

bidding domain
type: SequenceAnnotation
persistent identity: annotation2006751
display id: annotation2006751
version: 1
top level: BBa_K294205
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K294205/annotation2006751/range2006751/1

https://synbiohub.org/public/igem/BBa_K294205/annotation2006751/range2006751/1
type: Range
persistent identity: range2006751
display id: range2006751
version: 1
direction: direction/forward
top level: BBa_K294205
start: 59
end: 350
orientation: inline

BBa_K1486041
type: ComponentDefinition
persistent identity: BBa K1486041
display id: BBa_K1486041
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: sfGFPC + CpxR
creator: Ione Pla Puigsubir??
created: 2014-09-26T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: To edit
mutable notes: To edit
mutable provenance: To edit
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K1486039
component: BBa_I0500
component: BBa_B0034
component: BBa_K1486000
component: BBa_K1486037
sequence annotation: BBa_I0500
sequence annotation: BBa_B0034
sequence annotation: BBa_K1486039
sequence annotation: BBa_K1486000
sequence annotation: BBa_K1486037
sequence: https://synbiohub.org/public/igem/BBa_K1486041_sequence/1

BBa_K1486039
type: Component
persistent identity: component2387212
display id: component2387212
version: 1
top level: BBa_K1486041
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486039/1

BBa_I0500
type: Component
persistent identity: component2387209
display id: component2387209
version: 1
top level: BBa_K1486041
access: public
definition: https://synbiohub.org/public/igem/BBa_I0500/1

BBa_B0034
type: Component
persistent identity: component2387211
display id: component2387211
version: 1
top level: BBa_K1486041
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K1486000
type: Component
persistent identity: component2387217
display id: component2387217
version: 1
top level: BBa_K1486041
access: public
definition: BBa_K1486000

BBa_K1486037
type: Component
persistent identity: component2387213
display id: component2387213
version: 1
top level: BBa_K1486041
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486037/1

BBa_I0500
type: SequenceAnnotation
persistent identity: annotation2387209
display id: annotation2387209
version: 1
top level: BBa_K1486041
location: https://synbiohub.org/public/igem/BBa_K1486041/annotation2387209/range2387209/1
component: BBa_I0500

https://synbiohub.org/public/igem/BBa_K1486041/annotation2387209/range2387209/1
type: Range
persistent identity: range2387209
display id: range2387209
version: 1
direction: direction/reverse
top level: BBa_K1486041
start: 1
end: 1210
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2387211
display id: annotation2387211
version: 1
top level: BBa_K1486041
location: https://synbiohub.org/public/igem/BBa_K1486041/annotation2387211/range2387211/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1486041/annotation2387211/range2387211/1
type: Range
persistent identity: range2387211
display id: range2387211
version: 1
direction: direction/reverse
top level: BBa_K1486041
start: 1211
end: 1222
orientation: inline

BBa_K1486039
type: SequenceAnnotation
persistent identity: annotation2387212
display id: annotation2387212
version: 1
top level: BBa_K1486041
location: https://synbiohub.org/public/igem/BBa_K1486041/annotation2387212/range2387212/1
component: BBa_K1486039

https://synbiohub.org/public/igem/BBa_K1486041/annotation2387212/range2387212/1
type: Range
persistent identity: range2387212
display id: range2387212
version: 1
direction: direction/reverse
top level: BBa_K1486041
start: 1223
end: 1294
orientation: inline

BBa_K1486000
type: SequenceAnnotation
persistent identity: annotation2387217
display id: annotation2387217
version: 1
top level: BBa_K1486041
location: https://synbiohub.org/public/igem/BBa_K1486041/annotation2387217/range2387217/1
component: BBa_K1486000

https://synbiohub.org/public/igem/BBa_K1486041/annotation2387217/range2387217/1
type: Range
persistent identity: range2387217
display id: range2387217
version: 1
direction: direction/reverse
top level: BBa_K1486041
start: 1334
end: 2035
orientation: inline

BBa_K1486037
type: SequenceAnnotation
persistent identity: annotation2387213
display id: annotation2387213
version: 1
top level: BBa_K1486041
location: https://synbiohub.org/public/igem/BBa_K1486041/annotation2387213/range2387213/1
component: BBa_K1486037

https://synbiohub.org/public/igem/BBa_K1486041/annotation2387213/range2387213/1
type: Range
persistent identity: range2387213
display id: range2387213
version: 1
direction: direction/reverse
top level: BBa_K1486041
start: 1295
end: 1333
orientation: inline

BBa_K1031410
type: ComponentDefinition
persistent identity: BBa K1031410
display id: BBa_K1031410
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: HcaR-Terminator
creator: He Shuaixin
created: 2013-09-08T11:00:00Z
modified: 2015-05-08T01:08:47Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1338_
m user id: 0
owner id: 15843
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: HcaR-TT
mutable notes: molecular cloning
mutable provenance: PCR genome E.coli
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: BBa_B0034
sequence annotation: HcaR
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_K1031410_sequence/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2357450
display id: annotation2357450
version: 1
top level: BBa_K1031410
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1031410/annotation2357450/range2357450/1

https://synbiohub.org/public/igem/BBa_K1031410/annotation2357450/range2357450/1
type: Range
persistent identity: range2357450
display id: range2357450
version: 1
direction: direction/forward
top level: BBa_K1031410
start: 1
end: 12
orientation: inline

HcaR
type: SequenceAnnotation
persistent identity: annotation2357451
display id: annotation2357451
version: 1
top level: BBa_K1031410
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1031410/annotation2357451/range2357451/1

https://synbiohub.org/public/igem/BBa_K1031410/annotation2357451/range2357451/1
type: Range
persistent identity: range2357451
display id: range2357451
version: 1
direction: direction/forward
top level: BBa_K1031410
start: 19
end: 909
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2357452
display id: annotation2357452
version: 1
top level: BBa_K1031410
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1031410/annotation2357452/range2357452/1

https://synbiohub.org/public/igem/BBa_K1031410/annotation2357452/range2357452/1
type: Range
persistent identity: range2357452
display id: range2357452
version: 1
direction: direction/forward
top level: BBa_K1031410
start: 918
end: 1046
orientation: inline

BBa_J120015
type: ComponentDefinition
persistent identity: BBa J120015
display id: BBa_J120015
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Coding sequence for PAX3, a regulatory transcription factor in H. sapiens
creator: Brett Fuller, Austin Jones, Jeremy Gerbig, Sindhu Adhikari, Coby Turner
created: 2012-03-28T11:00:00Z
modified: 2015-08-31T04:08:29Z
discontinued: false
dominant: false
experience: experience/None
group u list: _617_
m user id: 0
owner id: 7766
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Contains coding sequence for PAX3, a transcription factor which binds to the Mitf promoter. When expressed in a system with the Mitf promoter present, will induce production of target gene.
mutable notes: N/A
mutable provenance: H. sapien
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_J120015_sequence/1

BBa_K2023010
type: ComponentDefinition
persistent identity: BBa K2023010
display id: BBa_K2023010
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: XylR gene with His tag
creator: C??lia Chenebault
created: 2016-10-13T11:00:00Z
modified: 2016-10-20T04:34:10Z
discontinued: false
dominant: false
group u list: _2490_
m user id: 30356
owner id: 30230
owning group id: 9
status: status/Planning
bookmark: false
mutable description: XylR gene with His tag
mutable notes: blabla
mutable provenance: IDT synthesis
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: XylR
sequence annotation: STOP XylR
sequence annotation: Linkage
sequence annotation: His Tag
sequence: https://synbiohub.org/public/igem/BBa_K2023010_sequence/1

XylR
type: SequenceAnnotation
persistent identity: annotation2519773
display id: annotation2519773
version: 1
top level: BBa_K2023010
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K2023010/annotation2519773/range2519773/1

https://synbiohub.org/public/igem/BBa_K2023010/annotation2519773/range2519773/1
type: Range
persistent identity: range2519773
display id: range2519773
version: 1
direction: direction/forward
top level: BBa_K2023010
start: 1
end: 1698
orientation: inline

STOP XylR
type: SequenceAnnotation
persistent identity: annotation2519778
display id: annotation2519778
version: 1
top level: BBa_K2023010
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K2023010/annotation2519778/range2519778/1

https://synbiohub.org/public/igem/BBa_K2023010/annotation2519778/range2519778/1
type: Range
persistent identity: range2519778
display id: range2519778
version: 1
direction: direction/forward
top level: BBa_K2023010
start: 1725
end: 1728
orientation: inline

Linkage
type: SequenceAnnotation
persistent identity: annotation2519776
display id: annotation2519776
version: 1
top level: BBa_K2023010
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K2023010/annotation2519776/range2519776/1

https://synbiohub.org/public/igem/BBa_K2023010/annotation2519776/range2519776/1
type: Range
persistent identity: range2519776
display id: range2519776
version: 1
direction: direction/forward
top level: BBa_K2023010
start: 1699
end: 1708
orientation: inline

His Tag
type: SequenceAnnotation
persistent identity: annotation2519777
display id: annotation2519777
version: 1
top level: BBa_K2023010
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K2023010/annotation2519777/range2519777/1

https://synbiohub.org/public/igem/BBa_K2023010/annotation2519777/range2519777/1
type: Range
persistent identity: range2519777
display id: range2519777
version: 1
direction: direction/forward
top level: BBa_K2023010
start: 1708
end: 1726
orientation: inline

luxr
type: ComponentDefinition
persistent identity: BBa C0062
display id: BBa_C0062
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: luxR repressor/activator, (no LVA?)
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:23Z
discontinued: false
dominant: true
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: In complex with HSL, LuxR binds to the Lux promoter, activating transcription from Pr <bb_part>BBa_R0062</bb_part>, and repressing transcription from Pl <bb_part>BBa_R0063</bb_part>. The lux cassette of V. fischeri contains a left and a right promoter. The right promoter gives weak constitutive expression of downstream genes.This expression is up-regulated by the action of the Lux activator, LuxR complexed to HSL. Two molecules of LuxR protein form a complex with two molecules the signalling compound homoserine lactone (HSL). This complex binds to a palindromic site on the promoter, increasing the rate of transcription.
mutable notes: 2 silent point mutants were introduced in the coding sequence to remove internal XbaI and PstI sites. Mutation sites were chosen to replace codons commonly used in E. coli with codons used at a similar frequency.
mutable provenance: V. fischeri <genbank>AF170104</genbank>
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: BBa_C0062
sequence annotation: prefix
sequence annotation: luxR
sequence annotation: T
sequence annotation: A
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C0062_sequence/1

BBa_C0062
type: SequenceAnnotation
persistent identity: annotation7039
display id: annotation7039
version: 1
top level: luxr
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_C0062/annotation7039/range7039/1

https://synbiohub.org/public/igem/BBa_C0062/annotation7039/range7039/1
type: Range
persistent identity: range7039
display id: range7039
version: 1
direction: direction/forward
top level: luxr
start: 1
end: 756
orientation: inline

prefix
type: SequenceAnnotation
persistent identity: annotation1762
display id: annotation1762
version: 1
top level: luxr
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_C0062/annotation1762/range1762/1

https://synbiohub.org/public/igem/BBa_C0062/annotation1762/range1762/1
type: Range
persistent identity: range1762
display id: range1762
version: 1
direction: direction/forward
top level: luxr
start: 1
end: 2
orientation: inline

luxR
type: SequenceAnnotation
persistent identity: annotation1766
display id: annotation1766
version: 1
top level: luxr
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_C0062/annotation1766/range1766/1

https://synbiohub.org/public/igem/BBa_C0062/annotation1766/range1766/1
type: Range
persistent identity: range1766
display id: range1766
version: 1
direction: direction/forward
top level: luxr
start: 1
end: 750
orientation: inline

T
type: SequenceAnnotation
persistent identity: annotation1764
display id: annotation1764
version: 1
top level: luxr
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_C0062/annotation1764/range1764/1

https://synbiohub.org/public/igem/BBa_C0062/annotation1764/range1764/1
type: Range
persistent identity: range1764
display id: range1764
version: 1
direction: direction/forward
top level: luxr
start: 174
end: 174
orientation: inline

A
type: SequenceAnnotation
persistent identity: annotation1765
display id: annotation1765
version: 1
top level: luxr
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_C0062/annotation1765/range1765/1

https://synbiohub.org/public/igem/BBa_C0062/annotation1765/range1765/1
type: Range
persistent identity: range1765
display id: range1765
version: 1
direction: direction/forward
top level: luxr
start: 492
end: 492
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213986
display id: annotation2213986
version: 1
top level: luxr
role: SO:0000807
role: feature/barcode
location: https://synbiohub.org/public/igem/BBa_C0062/annotation2213986/range2213986/1

https://synbiohub.org/public/igem/BBa_C0062/annotation2213986/range2213986/1
type: Range
persistent identity: range2213986
display id: range2213986
version: 1
direction: direction/forward
top level: luxr
start: 757
end: 781
orientation: inline

BBa_K1639002
type: ComponentDefinition
persistent identity: BBa K1639002
display id: BBa_K1639002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: GadE
creator: Mustafa Yılmaz
created: 2015-09-11T11:00:00Z
modified: 2015-09-18T06:54:04Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2056_
m user id: 20835
owner id: 20835
owning group id: 9
status: status/Planning
bookmark: true
mutable description: This part is activator of proteins responsible for acid resistance in E.coli. GadE is transcription factor of Gad operon and Gad operon express Glutamate Dependent Acid Resistance genes gadA, gadB, gadC.
mutable notes: none
mutable provenance: Escherichia Coli K-12 strain
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: GadE
sequence annotation: Start Codon
sequence annotation: Stop Codon
sequence: https://synbiohub.org/public/igem/BBa_K1639002_sequence/1

GadE
type: SequenceAnnotation
persistent identity: annotation2452830
display id: annotation2452830
version: 1
top level: BBa_K1639002
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1639002/annotation2452830/range2452830/1

https://synbiohub.org/public/igem/BBa_K1639002/annotation2452830/range2452830/1
type: Range
persistent identity: range2452830
display id: range2452830
version: 1
direction: direction/forward
top level: BBa_K1639002
start: 9
end: 536
orientation: inline

Start Codon
type: SequenceAnnotation
persistent identity: annotation2452829
display id: annotation2452829
version: 1
top level: BBa_K1639002
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1639002/annotation2452829/range2452829/1

https://synbiohub.org/public/igem/BBa_K1639002/annotation2452829/range2452829/1
type: Range
persistent identity: range2452829
display id: range2452829
version: 1
direction: direction/forward
top level: BBa_K1639002
start: 9
end: 11
orientation: inline

Stop Codon
type: SequenceAnnotation
persistent identity: annotation2452831
display id: annotation2452831
version: 1
top level: BBa_K1639002
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1639002/annotation2452831/range2452831/1

https://synbiohub.org/public/igem/BBa_K1639002/annotation2452831/range2452831/1
type: Range
persistent identity: range2452831
display id: range2452831
version: 1
direction: direction/forward
top level: BBa_K1639002
start: 534
end: 539
orientation: inline

BBa_K1031018
type: ComponentDefinition
persistent identity: BBa K1031018
display id: BBa_K1031018
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Psal-B0034-RiboJ-φR73δ
creator: He Shuaixin
created: 2013-10-21T11:00:00Z
modified: 2015-05-08T01:08:47Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1338_
m user id: 0
owner id: 15843
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Psal-φR73Δ
mutable notes: molecular cloning
mutable provenance: φR73
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: phiR73delta
sequence annotation: Psal
sequence: https://synbiohub.org/public/igem/BBa_K1031018_sequence/1

phiR73delta
type: SequenceAnnotation
persistent identity: annotation2370290
display id: annotation2370290
version: 1
top level: BBa_K1031018
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1031018/annotation2370290/range2370290/1

https://synbiohub.org/public/igem/BBa_K1031018/annotation2370290/range2370290/1
type: Range
persistent identity: range2370290
display id: range2370290
version: 1
direction: direction/forward
top level: BBa_K1031018
start: 235
end: 480
orientation: inline

Psal
type: SequenceAnnotation
persistent identity: annotation2370289
display id: annotation2370289
version: 1
top level: BBa_K1031018
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1031018/annotation2370289/range2370289/1

https://synbiohub.org/public/igem/BBa_K1031018/annotation2370289/range2370289/1
type: Range
persistent identity: range2370289
display id: range2370289
version: 1
direction: direction/forward
top level: BBa_K1031018
start: 1
end: 228
orientation: inline

BBa_K1520020
type: ComponentDefinition
persistent identity: BBa K1520020
display id: BBa_K1520020
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Pcons2-rbs-lacI-Ter-Plac-rbs-luxI-Ter-Pcons2-rbs-luxR-Ter
creator: yue wu
created: 2014-09-21T11:00:00Z
modified: 2015-05-08T01:10:49Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1902_
m user id: 0
owner id: 21318
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Pcons2-rbs-lacI-Ter-Plac-rbs-luxI-Ter It produces luxI when there is enough IPTG.
mutable notes: None
mutable provenance: Pcons2, lacI, Plac, luxI, Ter
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1520003
component: BBa_K1520019
sequence annotation: BBa_K1520019
sequence annotation: BBa_K1520003
sequence: https://synbiohub.org/public/igem/BBa_K1520020_sequence/1

BBa_K1520003
type: Component
persistent identity: component2385116
display id: component2385116
version: 1
top level: BBa_K1520020
access: public
definition: BBa_K1520003

BBa_K1520019
type: Component
persistent identity: component2385110
display id: component2385110
version: 1
top level: BBa_K1520020
access: public
definition: https://synbiohub.org/public/igem/BBa_K1520019/1

BBa_K1520019
type: SequenceAnnotation
persistent identity: annotation2385110
display id: annotation2385110
version: 1
top level: BBa_K1520020
location: https://synbiohub.org/public/igem/BBa_K1520020/annotation2385110/range2385110/1
component: BBa_K1520019

https://synbiohub.org/public/igem/BBa_K1520020/annotation2385110/range2385110/1
type: Range
persistent identity: range2385110
display id: range2385110
version: 1
direction: direction/reverse
top level: BBa_K1520020
start: 1
end: 2365
orientation: inline

BBa_K1520003
type: SequenceAnnotation
persistent identity: annotation2385116
display id: annotation2385116
version: 1
top level: BBa_K1520020
location: https://synbiohub.org/public/igem/BBa_K1520020/annotation2385116/range2385116/1
component: BBa_K1520003

https://synbiohub.org/public/igem/BBa_K1520020/annotation2385116/range2385116/1
type: Range
persistent identity: range2385116
display id: range2385116
version: 1
direction: direction/reverse
top level: BBa_K1520020
start: 2374
end: 3352
orientation: inline

BBa_K389411
type: ComponentDefinition
persistent identity: BBa K389411
display id: BBa_K389411
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: vir promoter + sensitivity tuner + luciferase
creator: Jonas Aretz
created: 2010-10-10T11:00:00Z
modified: 2015-05-08T01:12:19Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _517_
m user id: 0
owner id: 6670
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part combines a mutated ''virG'' response regulator from the VirA/G receptor system under the control of a constitutive promoter with a sensitivity tuner before a luciferase gene under the control of a ''vir'' promoter. The sensitivity tuner enhances a signal from a promoter so it enhances the readout luciferase.
mutable notes: sensitivity tuner to enhance the luciferase signal
mutable provenance: * luciferase from pGL4.10luc2 (Promega) * sensitivity tuner part, terminators and constitutive promoter from the partsregistry * ''virG'' was synthesized by Mr. Gene * ''vir'' promoter from ''Agrobacterium tumefaciens'' C58, TI-plasmid
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K389003
component: BBa_K389401
component: BBa_B0017
component: BBa_J23110
component: BBa_K389002
sequence annotation: BBa_K389002
sequence annotation: BBa_K389401
sequence annotation: BBa_B0017
sequence annotation: BBa_K389003
sequence annotation: BBa_J23110
sequence: https://synbiohub.org/public/igem/BBa_K389411_sequence/1

BBa_K389003
type: Component
persistent identity: component2300736
display id: component2300736
version: 1
top level: BBa_K389411
access: public
definition: https://synbiohub.org/public/igem/BBa_K389003/1

BBa_K389401
type: Component
persistent identity: component2300760
display id: component2300760
version: 1
top level: BBa_K389411
access: public
definition: https://synbiohub.org/public/igem/BBa_K389401/1

BBa_B0017
type: Component
persistent identity: component2300731
display id: component2300731
version: 1
top level: BBa_K389411
access: public
definition: https://synbiohub.org/public/igem/BBa_B0017/1

BBa_J23110
type: Component
persistent identity: component2300722
display id: component2300722
version: 1
top level: BBa_K389411
access: public
definition: https://synbiohub.org/public/igem/BBa_J23110/1

BBa_K389002
type: Component
persistent identity: component2300726
display id: component2300726
version: 1
top level: BBa_K389411
access: public
definition: virG

BBa_K389002
type: SequenceAnnotation
persistent identity: annotation2300726
display id: annotation2300726
version: 1
top level: BBa_K389411
location: https://synbiohub.org/public/igem/BBa_K389411/annotation2300726/range2300726/1
component: BBa_K389002

https://synbiohub.org/public/igem/BBa_K389411/annotation2300726/range2300726/1
type: Range
persistent identity: range2300726
display id: range2300726
version: 1
direction: direction/reverse
top level: BBa_K389411
start: 44
end: 783
orientation: inline

BBa_K389401
type: SequenceAnnotation
persistent identity: annotation2300760
display id: annotation2300760
version: 1
top level: BBa_K389411
location: https://synbiohub.org/public/igem/BBa_K389411/annotation2300760/range2300760/1
component: BBa_K389401

https://synbiohub.org/public/igem/BBa_K389411/annotation2300760/range2300760/1
type: Range
persistent identity: range2300760
display id: range2300760
version: 1
direction: direction/reverse
top level: BBa_K389411
start: 1134
end: 4020
orientation: inline

BBa_B0017
type: SequenceAnnotation
persistent identity: annotation2300731
display id: annotation2300731
version: 1
top level: BBa_K389411
location: https://synbiohub.org/public/igem/BBa_K389411/annotation2300731/range2300731/1
component: BBa_B0017

https://synbiohub.org/public/igem/BBa_K389411/annotation2300731/range2300731/1
type: Range
persistent identity: range2300731
display id: range2300731
version: 1
direction: direction/reverse
top level: BBa_K389411
start: 792
end: 959
orientation: inline

BBa_K389003
type: SequenceAnnotation
persistent identity: annotation2300736
display id: annotation2300736
version: 1
top level: BBa_K389411
location: https://synbiohub.org/public/igem/BBa_K389411/annotation2300736/range2300736/1
component: BBa_K389003

https://synbiohub.org/public/igem/BBa_K389411/annotation2300736/range2300736/1
type: Range
persistent identity: range2300736
display id: range2300736
version: 1
direction: direction/reverse
top level: BBa_K389411
start: 968
end: 1125
orientation: inline

BBa_J23110
type: SequenceAnnotation
persistent identity: annotation2300722
display id: annotation2300722
version: 1
top level: BBa_K389411
location: https://synbiohub.org/public/igem/BBa_K389411/annotation2300722/range2300722/1
component: BBa_J23110

https://synbiohub.org/public/igem/BBa_K389411/annotation2300722/range2300722/1
type: Range
persistent identity: range2300722
display id: range2300722
version: 1
direction: direction/reverse
top level: BBa_K389411
start: 1
end: 35
orientation: inline

BB123
type: ComponentDefinition
persistent identity: BBa K2023001
display id: BBa_K2023001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Biosensor device for BTEX detection
creator: Celia Chenebault, Camille Soucies, Benjamin Piot
created: 2016-10-13T11:00:00Z
modified: 2016-10-20T12:05:53Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2490_
m user id: 30230
owner id: 30230
owning group id: 9
status: status/Planning
bookmark: false
mutable description: The Pr promoter is a constitutive promoter driving the transcription of the XylR gene, coding for the XylR protein. The XylR protein, a transcriptional regulatory protein for the Pu promoter, is activated by aromatic hydrocarbons that carry a methyl group (like toluene and xylene). In our biosensor the Pu promoter allows the transcription of the bioluminescent reporter gene GLuc, coding for the Gaussia luciferase. When this enzyme reacts with its coelenterazine substrate it emits luminescence.
mutable notes: Site directed mutagenesis to overcome the mutation in the start codon of XylR
mutable provenance: IDT synthesis in 3 parts that were then assembled
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Measurement
component: BBa_K2023003
component: BBa_K2023002
sequence annotation: BBa_K2023003
sequence annotation: BBa_K2023002
sequence: https://synbiohub.org/public/igem/BBa_K2023001_sequence/1

BBa_K2023003
type: Component
persistent identity: component2518727
display id: component2518727
version: 1
top level: BB123
access: public
definition: https://synbiohub.org/public/igem/BBa_K2023003/1

BBa_K2023002
type: Component
persistent identity: component2518713
display id: component2518713
version: 1
top level: BB123
access: public
definition: https://synbiohub.org/public/igem/BBa_K2023002/1

BBa_K2023003
type: SequenceAnnotation
persistent identity: annotation2518727
display id: annotation2518727
version: 1
top level: BB123
location: https://synbiohub.org/public/igem/BBa_K2023001/annotation2518727/range2518727/1
component: BBa_K2023003

https://synbiohub.org/public/igem/BBa_K2023001/annotation2518727/range2518727/1
type: Range
persistent identity: range2518727
display id: range2518727
version: 1
direction: direction/reverse
top level: BB123
start: 2252
end: 3261
orientation: inline

BBa_K2023002
type: SequenceAnnotation
persistent identity: annotation2518713
display id: annotation2518713
version: 1
top level: BB123
location: https://synbiohub.org/public/igem/BBa_K2023001/annotation2518713/range2518713/1
component: BBa_K2023002

https://synbiohub.org/public/igem/BBa_K2023001/annotation2518713/range2518713/1
type: Range
persistent identity: range2518713
display id: range2518713
version: 1
direction: direction/reverse
top level: BB123
start: 1
end: 2243
orientation: inline

BBa_K1982005
type: ComponentDefinition
persistent identity: BBa K1982005
display id: BBa_K1982005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CRY2-VP64(Prokaryotic LACE system)
creator: Zexu Li
created: 2016-09-11T11:00:00Z
modified: 2016-10-18T10:38:55Z
discontinued: false
dominant: false
group u list: _2449_
m user id: 30176
owner id: 30176
owning group id: 9
status: status/Planning
bookmark: false
mutable description: The CRY2/CIBN interaction is entirely genetically encoded. The binding reverses within minutes in the dark, allowing rapid shutoff of transcription by placing samples in the dark. This fusion protein is for use in LACE(light-activated CRISPR/Cas9 effector) system, and a VP64 fused to its C terminus. To regulate DNA transcription by blue light, the system is based on CRY2/CIBN interaction in which a light-mediated protein interaction brings together two protein (tCas9 and an activation domain VP64) . If we remove the stimulation of blue light, dark reversion of CRY2 will dissociate the interaction with CIBN and shut off transcription.
mutable notes: no
mutable provenance: CRY2 and CIB1 are extracted from Arabidopsis thaliana. tCas9 is from GE Share company. VP64 is synthetic construct.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
sequence annotation: VP16
sequence annotation: ATG
sequence annotation: SV40 NLS
sequence annotation: TAATAA
sequence annotation: Prokaryotic rbs
sequence annotation: FLAG
sequence annotation: HA
sequence annotation: CRY2
sequence annotation: Linker
sequence: https://synbiohub.org/public/igem/BBa_K1982005_sequence/1

VP16
type: SequenceAnnotation
persistent identity: annotation2517266
display id: annotation2517266
version: 1
top level: BBa_K1982005
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1982005/annotation2517266/range2517266/1

https://synbiohub.org/public/igem/BBa_K1982005/annotation2517266/range2517266/1
type: Range
persistent identity: range2517266
display id: range2517266
version: 1
direction: direction/forward
top level: BBa_K1982005
start: 1876
end: 1914
orientation: inline

ATG
type: SequenceAnnotation
persistent identity: annotation2517263
display id: annotation2517263
version: 1
top level: BBa_K1982005
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1982005/annotation2517263/range2517263/1

https://synbiohub.org/public/igem/BBa_K1982005/annotation2517263/range2517263/1
type: Range
persistent identity: range2517263
display id: range2517263
version: 1
direction: direction/forward
top level: BBa_K1982005
start: 19
end: 21
orientation: inline

SV40 NLS
type: SequenceAnnotation
persistent identity: annotation2517265
display id: annotation2517265
version: 1
top level: BBa_K1982005
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1982005/annotation2517265/range2517265/1

https://synbiohub.org/public/igem/BBa_K1982005/annotation2517265/range2517265/1
type: Range
persistent identity: range2517265
display id: range2517265
version: 1
direction: direction/forward
top level: BBa_K1982005
start: 1855
end: 1875
orientation: inline

TAATAA
type: SequenceAnnotation
persistent identity: annotation2517273
display id: annotation2517273
version: 1
top level: BBa_K1982005
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1982005/annotation2517273/range2517273/1

https://synbiohub.org/public/igem/BBa_K1982005/annotation2517273/range2517273/1
type: Range
persistent identity: range2517273
display id: range2517273
version: 1
direction: direction/forward
top level: BBa_K1982005
start: 2095
end: 2100
orientation: inline

Prokaryotic rbs
type: SequenceAnnotation
persistent identity: annotation2517262
display id: annotation2517262
version: 1
top level: BBa_K1982005
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1982005/annotation2517262/range2517262/1

https://synbiohub.org/public/igem/BBa_K1982005/annotation2517262/range2517262/1
type: Range
persistent identity: range2517262
display id: range2517262
version: 1
direction: direction/forward
top level: BBa_K1982005
start: 1
end: 18
orientation: inline

VP16
type: SequenceAnnotation
persistent identity: annotation2517269
display id: annotation2517269
version: 1
top level: BBa_K1982005
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1982005/annotation2517269/range2517269/1

https://synbiohub.org/public/igem/BBa_K1982005/annotation2517269/range2517269/1
type: Range
persistent identity: range2517269
display id: range2517269
version: 1
direction: direction/forward
top level: BBa_K1982005
start: 1993
end: 2031
orientation: inline

VP16
type: SequenceAnnotation
persistent identity: annotation2517267
display id: annotation2517267
version: 1
top level: BBa_K1982005
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1982005/annotation2517267/range2517267/1

https://synbiohub.org/public/igem/BBa_K1982005/annotation2517267/range2517267/1
type: Range
persistent identity: range2517267
display id: range2517267
version: 1
direction: direction/forward
top level: BBa_K1982005
start: 1915
end: 1953
orientation: inline

VP16
type: SequenceAnnotation
persistent identity: annotation2517268
display id: annotation2517268
version: 1
top level: BBa_K1982005
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1982005/annotation2517268/range2517268/1

https://synbiohub.org/public/igem/BBa_K1982005/annotation2517268/range2517268/1
type: Range
persistent identity: range2517268
display id: range2517268
version: 1
direction: direction/forward
top level: BBa_K1982005
start: 1954
end: 1992
orientation: inline

FLAG
type: SequenceAnnotation
persistent identity: annotation2517272
display id: annotation2517272
version: 1
top level: BBa_K1982005
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1982005/annotation2517272/range2517272/1

https://synbiohub.org/public/igem/BBa_K1982005/annotation2517272/range2517272/1
type: Range
persistent identity: range2517272
display id: range2517272
version: 1
direction: direction/forward
top level: BBa_K1982005
start: 2071
end: 2094
orientation: inline

HA
type: SequenceAnnotation
persistent identity: annotation2517271
display id: annotation2517271
version: 1
top level: BBa_K1982005
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1982005/annotation2517271/range2517271/1

https://synbiohub.org/public/igem/BBa_K1982005/annotation2517271/range2517271/1
type: Range
persistent identity: range2517271
display id: range2517271
version: 1
direction: direction/forward
top level: BBa_K1982005
start: 2037
end: 2063
orientation: inline

CRY2
type: SequenceAnnotation
persistent identity: annotation2517264
display id: annotation2517264
version: 1
top level: BBa_K1982005
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1982005/annotation2517264/range2517264/1

https://synbiohub.org/public/igem/BBa_K1982005/annotation2517264/range2517264/1
type: Range
persistent identity: range2517264
display id: range2517264
version: 1
direction: direction/reverse
top level: BBa_K1982005
start: 22
end: 1854
orientation: inline

Linker
type: SequenceAnnotation
persistent identity: annotation2517270
display id: annotation2517270
version: 1
top level: BBa_K1982005
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1982005/annotation2517270/range2517270/1

https://synbiohub.org/public/igem/BBa_K1982005/annotation2517270/range2517270/1
type: Range
persistent identity: range2517270
display id: range2517270
version: 1
direction: direction/forward
top level: BBa_K1982005
start: 2032
end: 2037
orientation: inline

BBa_K1031913
type: ComponentDefinition
persistent identity: BBa K1031913
display id: BBa_K1031913
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: J23109-XylS-Terminator
creator: He Shuaixin
created: 2013-09-08T11:00:00Z
modified: 2015-05-08T01:08:48Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1338_
m user id: 0
owner id: 15843
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: 109-XylS-TT
mutable notes: molecular cloning
mutable provenance: synthesis Pseudomonas
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: B0015
sequence annotation: J23109
sequence annotation: XylS
sequence: https://synbiohub.org/public/igem/BBa_K1031913_sequence/1

B0015
type: SequenceAnnotation
persistent identity: annotation2360171
display id: annotation2360171
version: 1
top level: BBa_K1031913
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1031913/annotation2360171/range2360171/1

https://synbiohub.org/public/igem/BBa_K1031913/annotation2360171/range2360171/1
type: Range
persistent identity: range2360171
display id: range2360171
version: 1
direction: direction/forward
top level: BBa_K1031913
start: 1034
end: 1162
orientation: inline

J23109
type: SequenceAnnotation
persistent identity: annotation2360169
display id: annotation2360169
version: 1
top level: BBa_K1031913
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1031913/annotation2360169/range2360169/1

https://synbiohub.org/public/igem/BBa_K1031913/annotation2360169/range2360169/1
type: Range
persistent identity: range2360169
display id: range2360169
version: 1
direction: direction/forward
top level: BBa_K1031913
start: 1
end: 35
orientation: inline

XylS
type: SequenceAnnotation
persistent identity: annotation2360170
display id: annotation2360170
version: 1
top level: BBa_K1031913
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1031913/annotation2360170/range2360170/1

https://synbiohub.org/public/igem/BBa_K1031913/annotation2360170/range2360170/1
type: Range
persistent identity: range2360170
display id: range2360170
version: 1
direction: direction/forward
top level: BBa_K1031913
start: 36
end: 1027
orientation: inline

BBa_K1031917
type: ComponentDefinition
persistent identity: BBa K1031917
display id: BBa_K1031917
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: J23117-XylS-Terminator
creator: He Shuaixin
created: 2013-10-13T11:00:00Z
modified: 2015-05-08T01:08:48Z
discontinued: false
dominant: false
group u list: _1338_
m user id: 0
owner id: 15843
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: 117-XylS-TT
mutable notes: molecular cloning
mutable provenance: Pseudomonas.putida
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: B0015
sequence annotation: J23117
sequence annotation: XylS
sequence: https://synbiohub.org/public/igem/BBa_K1031917_sequence/1

B0015
type: SequenceAnnotation
persistent identity: annotation2369602
display id: annotation2369602
version: 1
top level: BBa_K1031917
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1031917/annotation2369602/range2369602/1

https://synbiohub.org/public/igem/BBa_K1031917/annotation2369602/range2369602/1
type: Range
persistent identity: range2369602
display id: range2369602
version: 1
direction: direction/forward
top level: BBa_K1031917
start: 1035
end: 1163
orientation: inline

J23117
type: SequenceAnnotation
persistent identity: annotation2369600
display id: annotation2369600
version: 1
top level: BBa_K1031917
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1031917/annotation2369600/range2369600/1

https://synbiohub.org/public/igem/BBa_K1031917/annotation2369600/range2369600/1
type: Range
persistent identity: range2369600
display id: range2369600
version: 1
direction: direction/forward
top level: BBa_K1031917
start: 1
end: 35
orientation: inline

XylS
type: SequenceAnnotation
persistent identity: annotation2369601
display id: annotation2369601
version: 1
top level: BBa_K1031917
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1031917/annotation2369601/range2369601/1

https://synbiohub.org/public/igem/BBa_K1031917/annotation2369601/range2369601/1
type: Range
persistent identity: range2369601
display id: range2369601
version: 1
direction: direction/forward
top level: BBa_K1031917
start: 63
end: 1028
orientation: inline

BBa_K1031311
type: ComponentDefinition
persistent identity: BBa K1031311
display id: BBa_K1031311
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: J23114-HbpR-Terminator
creator: He Shuaixin
created: 2013-10-12T11:00:00Z
modified: 2015-05-08T01:08:47Z
discontinued: false
dominant: false
group u list: _1338_
m user id: 0
owner id: 15843
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: 114-HbpR-TT
mutable notes: molecular cloning
mutable provenance: P.putida
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: J23114
sequence annotation: B0015
sequence annotation: HbpR
sequence: https://synbiohub.org/public/igem/BBa_K1031311_sequence/1

J23114
type: SequenceAnnotation
persistent identity: annotation2369597
display id: annotation2369597
version: 1
top level: BBa_K1031311
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1031311/annotation2369597/range2369597/1

https://synbiohub.org/public/igem/BBa_K1031311/annotation2369597/range2369597/1
type: Range
persistent identity: range2369597
display id: range2369597
version: 1
direction: direction/forward
top level: BBa_K1031311
start: 1
end: 35
orientation: inline

B0015
type: SequenceAnnotation
persistent identity: annotation2369599
display id: annotation2369599
version: 1
top level: BBa_K1031311
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1031311/annotation2369599/range2369599/1

https://synbiohub.org/public/igem/BBa_K1031311/annotation2369599/range2369599/1
type: Range
persistent identity: range2369599
display id: range2369599
version: 1
direction: direction/forward
top level: BBa_K1031311
start: 1782
end: 1910
orientation: inline

HbpR
type: SequenceAnnotation
persistent identity: annotation2369598
display id: annotation2369598
version: 1
top level: BBa_K1031311
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1031311/annotation2369598/range2369598/1

https://synbiohub.org/public/igem/BBa_K1031311/annotation2369598/range2369598/1
type: Range
persistent identity: range2369598
display id: range2369598
version: 1
direction: direction/forward
top level: BBa_K1031311
start: 63
end: 1713
orientation: inline

cinr
type: ComponentDefinition
persistent identity: BBa C0077
display id: BBa_C0077
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: cinR activator from Rhizobium leguminosarum (+LVA)
creator: crackdots
created: 2004-01-27T12:00:00Z
modified: 2015-08-31T04:07:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: In complex with O3-C14:1-HSL (made by BBa_C0076), CinR binds to the Cin promoter (BBa_R0077) and activates transcription.
mutable notes: The regulatory locus cinRI in Rhizobium leguminosarum conrols a network of quorum-sensing loci Lithgow, JK; Wilkinson, A; Hardman, A; Rodelas, B; Wisniewski-Dye, F; Williams, P; Downie, AJ MOL. MICROBIOLOGY 37(1): 81-97, 2000 Change log: original STOP: tag -> taATAA
mutable provenance: Rhizobium leguminosarum
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Help:Barcodes
sequence annotation: LVA
sequence annotation: cinR
sequence: https://synbiohub.org/public/igem/BBa_C0077_sequence/1

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2214000
display id: annotation2214000
version: 1
top level: cinr
role: SO:0000807
role: feature/barcode
location: https://synbiohub.org/public/igem/BBa_C0077/annotation2214000/range2214000/1

https://synbiohub.org/public/igem/BBa_C0077/annotation2214000/range2214000/1
type: Range
persistent identity: range2214000
display id: range2214000
version: 1
direction: direction/forward
top level: cinr
start: 763
end: 787
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation306600
display id: annotation306600
version: 1
top level: cinr
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_C0077/annotation306600/range306600/1

https://synbiohub.org/public/igem/BBa_C0077/annotation306600/range306600/1
type: Range
persistent identity: range306600
display id: range306600
version: 1
direction: direction/forward
top level: cinr
start: 724
end: 756
orientation: inline

cinR
type: SequenceAnnotation
persistent identity: annotation301112
display id: annotation301112
version: 1
top level: cinr
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_C0077/annotation301112/range301112/1

https://synbiohub.org/public/igem/BBa_C0077/annotation301112/range301112/1
type: Range
persistent identity: range301112
display id: range301112
version: 1
direction: direction/forward
top level: cinr
start: 1
end: 723
orientation: inline

BBa_K1486012
type: ComponentDefinition
persistent identity: BBa K1486012
display id: BBa_K1486012
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CpxR-IFP[1] under pBAD
creator: iGem EPFL 2014
created: 2014-08-31T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part has a fused protein downstream of an arabinose-inducible promoter: CpxR-IFP[1]. Biobrick BBa_K1486013 has CpxR-IFP[2] with an arabinose promoter. This construct was made in both orientations: BBa_K1486014 is IFP[1]-CpxR (with an arabinose promoter).
mutable notes: Both orientations were made, as well as for IFP[2] so all dimerizations could be tested (see main page for biobrick numbers). Flexible linkers were used to fuse both proteins, and an RBS was put before each one of them.
mutable provenance: To Edit
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0034
component: BBa_K1486004
component: BBa_K1486006
component: BBa_I0500
component: BBa_K1486000
sequence annotation: BBa_K1486006
sequence annotation: BBa_B0034
sequence annotation: BBa_I0500
sequence annotation: BBa_K1486004
sequence annotation: BBa_K1486000
sequence: https://synbiohub.org/public/igem/BBa_K1486012_sequence/1

BBa_B0034
type: Component
persistent identity: component2382287
display id: component2382287
version: 1
top level: BBa_K1486012
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K1486004
type: Component
persistent identity: component2382292
display id: component2382292
version: 1
top level: BBa_K1486012
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486004/1

BBa_K1486006
type: Component
persistent identity: component2382293
display id: component2382293
version: 1
top level: BBa_K1486012
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486006/1

BBa_I0500
type: Component
persistent identity: component2382285
display id: component2382285
version: 1
top level: BBa_K1486012
access: public
definition: https://synbiohub.org/public/igem/BBa_I0500/1

BBa_K1486000
type: Component
persistent identity: component2382291
display id: component2382291
version: 1
top level: BBa_K1486012
access: public
definition: BBa_K1486000

BBa_K1486006
type: SequenceAnnotation
persistent identity: annotation2382293
display id: annotation2382293
version: 1
top level: BBa_K1486012
location: https://synbiohub.org/public/igem/BBa_K1486012/annotation2382293/range2382293/1
component: BBa_K1486006

https://synbiohub.org/public/igem/BBa_K1486012/annotation2382293/range2382293/1
type: Range
persistent identity: range2382293
display id: range2382293
version: 1
direction: direction/reverse
top level: BBa_K1486012
start: 1955
end: 2536
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2382287
display id: annotation2382287
version: 1
top level: BBa_K1486012
location: https://synbiohub.org/public/igem/BBa_K1486012/annotation2382287/range2382287/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1486012/annotation2382287/range2382287/1
type: Range
persistent identity: range2382287
display id: range2382287
version: 1
direction: direction/reverse
top level: BBa_K1486012
start: 1211
end: 1222
orientation: inline

BBa_I0500
type: SequenceAnnotation
persistent identity: annotation2382285
display id: annotation2382285
version: 1
top level: BBa_K1486012
location: https://synbiohub.org/public/igem/BBa_K1486012/annotation2382285/range2382285/1
component: BBa_I0500

https://synbiohub.org/public/igem/BBa_K1486012/annotation2382285/range2382285/1
type: Range
persistent identity: range2382285
display id: range2382285
version: 1
direction: direction/reverse
top level: BBa_K1486012
start: 1
end: 1210
orientation: inline

BBa_K1486004
type: SequenceAnnotation
persistent identity: annotation2382292
display id: annotation2382292
version: 1
top level: BBa_K1486012
location: https://synbiohub.org/public/igem/BBa_K1486012/annotation2382292/range2382292/1
component: BBa_K1486004

https://synbiohub.org/public/igem/BBa_K1486012/annotation2382292/range2382292/1
type: Range
persistent identity: range2382292
display id: range2382292
version: 1
direction: direction/reverse
top level: BBa_K1486012
start: 1925
end: 1954
orientation: inline

BBa_K1486000
type: SequenceAnnotation
persistent identity: annotation2382291
display id: annotation2382291
version: 1
top level: BBa_K1486012
location: https://synbiohub.org/public/igem/BBa_K1486012/annotation2382291/range2382291/1
component: BBa_K1486000

https://synbiohub.org/public/igem/BBa_K1486012/annotation2382291/range2382291/1
type: Range
persistent identity: range2382291
display id: range2382291
version: 1
direction: direction/reverse
top level: BBa_K1486012
start: 1223
end: 1924
orientation: inline

BBa_K105001
type: ComponentDefinition
persistent identity: BBa K105001
display id: BBa_K105001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: VP16 - eucaryotic activation domain
creator: Manuel Gersbacher
created: 2008-10-11T11:00:00Z
modified: 2015-05-08T01:08:52Z
discontinued: false
dominant: false
experience: experience/None
group u list: _253_
m user id: 0
owner id: 3313
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: Originally VP16 is a protein from the herpes simplex virus. It activates the transcription of the late genes of this virus by the RNA-polymerase II of the eucaryotic host. In artificial systems VP16 is used in fusion with a DNA-binding domain. This DNA-binding domain is specific for a bait sequence. So the selective expression of genes with a promoter containing this bait sequence can be achieved.
mutable notes: To enable the fusion with other domains in frame the vector of this BioBrick has no base pair in between the restriction side and the BioBrick. <br\n> For more information about this issus, see Phillips, I.E. and Silver, P.A. "A new Biobrick Assembly Strategy Designed for Facile Protein Engineering." DSpace http://hdl.handle.net/1721.1/32535 (2006).
mutable provenance: We have constructed our BioBrick from the plasmid xxx, which was available in our hosting lab.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence: https://synbiohub.org/public/igem/BBa_K105001_sequence/1

rhlR
type: ComponentDefinition
persistent identity: BBa C0071
display id: BBa_C0071
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: rhlR repressor/activator from P. aeruginosa PA3477 (+LVA)
creator: Debra Lin, Srini Devadas, David Gray, Ronny Krashinsky, and Chris Zheng Liu, Boopers
created: 2004-01-26T12:00:00Z
modified: 2015-08-31T04:07:23Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Transcriptional regulator, in complex with N-butyryl-HSL, RhlR binds to the Rhl promoter
mutable notes: (1/28/04 Edit Jamboree) (0) No BB sites (1) ATG (2) TAATAA (3) Blast checks out (4) No codon changes (5) Maybe chassis compatability (check by experiment) (6) Codon usage not optimal (but looks OK) (7) ssrA tag added
mutable provenance: P. aeruginosa PA3477
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: rhlR
sequence annotation: Help:Barcodes
sequence annotation: LVA
sequence: https://synbiohub.org/public/igem/BBa_C0071_sequence/1

rhlR
type: SequenceAnnotation
persistent identity: annotation301192
display id: annotation301192
version: 1
top level: rhlR
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_C0071/annotation301192/range301192/1

https://synbiohub.org/public/igem/BBa_C0071/annotation301192/range301192/1
type: Range
persistent identity: range301192
display id: range301192
version: 1
direction: direction/forward
top level: rhlR
start: 1
end: 723
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213995
display id: annotation2213995
version: 1
top level: rhlR
role: SO:0000807
role: feature/barcode
location: https://synbiohub.org/public/igem/BBa_C0071/annotation2213995/range2213995/1

https://synbiohub.org/public/igem/BBa_C0071/annotation2213995/range2213995/1
type: Range
persistent identity: range2213995
display id: range2213995
version: 1
direction: direction/forward
top level: rhlR
start: 763
end: 787
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation306523
display id: annotation306523
version: 1
top level: rhlR
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_C0071/annotation306523/range306523/1

https://synbiohub.org/public/igem/BBa_C0071/annotation306523/range306523/1
type: Range
persistent identity: range306523
display id: range306523
version: 1
direction: direction/forward
top level: rhlR
start: 724
end: 756
orientation: inline

BBa_K1467205
type: ComponentDefinition
persistent identity: BBa K1467205
display id: BBa_K1467205
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: AvrBS3 TAL Effector - Induces BS3
creator: Cara Deal
created: 2014-09-23T11:00:00Z
modified: 2015-05-29T01:38:58Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1846_
m user id: 4206
owner id: 21550
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: TALE that induces the BS3 promoter. The part is compatible with the GoldenGate MoClo Assembly Standard (free from BsaI and BpiI recognition sequences) Codon-optimised for expression in dicotyledenous plants
mutable notes: Domestication for GoldenGate by removal of internal BsaI and BpiI recognition sequences. Codon-optimised for expression in dicotyledenous plants
mutable provenance: Xanthomonas vesicatoria
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1467205_sequence/1

BBa_K1486000
type: ComponentDefinition
persistent identity: BBa K1486000
display id: BBa_K1486000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CpxR coding region
creator: iGem EPFL 2014
created: 2014-08-11T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: CpxR is a transciption factor that dimerizes and becomes active when phosphorylated by the transmembrane histidine kinase CpxA. CpxA in turn becomes active when the bacterial cell is submitted to different kinds of shock (pH, temperature, pressure, protein misfolding in the inter-membrane space...).
mutable notes: When making composite parts with CpxR that are supposed to dimerize, we had to take care about the positioning of the other elements regarding the parallel dimerization of CpxR.
mutable provenance: The part has been extracted from the E coli genome.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: START
sequence annotation: CpxR
sequence annotation: STOP
sequence: https://synbiohub.org/public/igem/BBa_K1486000_sequence/1

START
type: SequenceAnnotation
persistent identity: annotation2382022
display id: annotation2382022
version: 1
top level: BBa_K1486000
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1486000/annotation2382022/range2382022/1

https://synbiohub.org/public/igem/BBa_K1486000/annotation2382022/range2382022/1
type: Range
persistent identity: range2382022
display id: range2382022
version: 1
direction: direction/forward
top level: BBa_K1486000
start: 1
end: 3
orientation: inline

CpxR
type: SequenceAnnotation
persistent identity: annotation2382021
display id: annotation2382021
version: 1
top level: BBa_K1486000
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1486000/annotation2382021/range2382021/1

https://synbiohub.org/public/igem/BBa_K1486000/annotation2382021/range2382021/1
type: Range
persistent identity: range2382021
display id: range2382021
version: 1
direction: direction/forward
top level: BBa_K1486000
start: 1
end: 702
orientation: inline

STOP
type: SequenceAnnotation
persistent identity: annotation2382023
display id: annotation2382023
version: 1
top level: BBa_K1486000
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1486000/annotation2382023/range2382023/1

https://synbiohub.org/public/igem/BBa_K1486000/annotation2382023/range2382023/1
type: Range
persistent identity: range2382023
display id: range2382023
version: 1
direction: direction/forward
top level: BBa_K1486000
start: 699
end: 702
orientation: inline

BBa_K332021
type: ComponentDefinition
persistent identity: BBa K332021
display id: BBa_K332021
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Ptet + RBS + LuxR
creator: Chen-Hsi Tang
created: 2010-10-23T11:00:00Z
modified: 2015-05-08T01:12:05Z
discontinued: false
dominant: false
experience: experience/None
group u list: _452_
m user id: 0
owner id: 7483
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: In this portion of the design, we aim to achieve population control by combining a cell-signaling system (luxR/luxI) and suicidal gene for controlling the number of E.coli. The population controlling function in the engineered E.coli can warn us of high-density E. coli congregations by emitting fluorescent proteins. Green fluorescent light means the Cry circuit is functional and the population density is under control, while red fluorescent gene signals E.coli overpopulation.
mutable notes: (I) Strand C (BBa_ K332021) 1. Strand C is synthesized from three fragments ??? (1) Ptet (2) RBS+LuxR (3) RBS+LuxI+double ter sites. 2. All of them are in the form of plasmids, so we digest them by appropriate restriction enzymes. 3. We first combine the last two fragments by ligation. The procedure is as follows: (1)Add the respective digestion products, vector plasmid, buffer, ligase to ddwater (2)React at 16??C for 30 minutes (3)Inactivate the ligase by incubation it at 80??C for 20 minutes (4)Store the products at 4??C 4. The products of ligation are then transformed into competent cells. The procedure is as follows: (1)2??l plasmids(ligation products) are added to 33??l competent cells (2)Shake gently for one second, and cool it on ice (3)Incubate it in 42??C water bath for 45 seconds (4)Spread the liquid to plate which corresponding antibiotics have been added to (5)37??C overnight 5. The next day we do a colony PCR for the transformed cells and have the PCR products electrophoresis to make sure that the two fragments have been combined correctly. 6. Now we separate the plasmids(RBS+LuxR+RBS+LuxI+double ter sites) from E. Coli, and digest it with restriction enzyme. 7. The first fragment (Ptet) is then combined with the above product by ligation, and transformed into competent cells by the same we have done early. 8. Because the length of Ptet to the remain segment is relatively too short, we cannot confirm whether they have combined correctly by just do an electrophoresis of the colony PCR products. If the electrophoresis result seems like that they have combined, we then send the product to sequencing.
mutable provenance: 1. LuxI protein (BBa_C0061) is the synthase that converts S-adenosylmethionine (SAM) into acyl-homoserine lactone (AHL). When the concentration of AHL reaches a threshold level, it binds to the N-terminal domain of a LuxR protein. Then the LuxR/AHL complex binds the Lux box within PtetR promoter (BBa_C0040), which then activates suicidal gene expression. 2. LuxR (BBa_C0062) is transcribed continuously by the constitutive promoter (BBa_J23106), and later works in conjunction with AHL. This design can control the concentration of LuxR protein in a steady phase, and the concentration of AHL/LuxR complex is only affected by the concentration of LuxI protein (BBa_C0061).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0034
component: BBa_R0040
component: BBa_C0062
sequence annotation: BBa_R0040
sequence annotation: BBa_C0062
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K332021_sequence/1

BBa_B0034
type: Component
persistent identity: component2247623
display id: component2247623
version: 1
top level: BBa_K332021
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_R0040
type: Component
persistent identity: component2247617
display id: component2247617
version: 1
top level: BBa_K332021
access: public
definition: https://synbiohub.org/public/igem/BBa_R0040/1

BBa_C0062
type: Component
persistent identity: component2247626
display id: component2247626
version: 1
top level: BBa_K332021
access: public
definition: luxr

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation2247617
display id: annotation2247617
version: 1
top level: BBa_K332021
location: https://synbiohub.org/public/igem/BBa_K332021/annotation2247617/range2247617/1
component: BBa_R0040

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type: Range
persistent identity: range2247617
display id: range2247617
version: 1
direction: direction/forward
top level: BBa_K332021
start: 1
end: 54
orientation: inline

BBa_C0062
type: SequenceAnnotation
persistent identity: annotation2247626
display id: annotation2247626
version: 1
top level: BBa_K332021
location: https://synbiohub.org/public/igem/BBa_K332021/annotation2247626/range2247626/1
component: BBa_C0062

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type: Range
persistent identity: range2247626
display id: range2247626
version: 1
direction: direction/forward
top level: BBa_K332021
start: 81
end: 836
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2247623
display id: annotation2247623
version: 1
top level: BBa_K332021
location: https://synbiohub.org/public/igem/BBa_K332021/annotation2247623/range2247623/1
component: BBa_B0034

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type: Range
persistent identity: range2247623
display id: range2247623
version: 1
direction: direction/reverse
top level: BBa_K332021
start: 63
end: 74
orientation: inline

BBa_K1486001
type: ComponentDefinition
persistent identity: BBa K1486001
display id: BBa_K1486001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Arabinose promoter + CpxR
creator: iGem EPFL 2014
created: 2014-08-11T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: The part contains CpxR with an upstream arabinose-inducible promoter.
mutable notes: The arabinose had to be upstream of the CpxR. Also, the sequence had to be tested because of a mutation, and it works perfectly.
mutable provenance: The CpxR comes from the genome of E coli, and the arabinose promoter comes from a biobrick.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_I0500
component: BBa_B0034
component: BBa_K1486000
sequence annotation: BBa_B0034
sequence annotation: BBa_K1486000
sequence annotation: BBa_I0500
sequence: https://synbiohub.org/public/igem/BBa_K1486001_sequence/1

BBa_I0500
type: Component
persistent identity: component2382072
display id: component2382072
version: 1
top level: BBa_K1486001
access: public
definition: https://synbiohub.org/public/igem/BBa_I0500/1

BBa_B0034
type: Component
persistent identity: component2382074
display id: component2382074
version: 1
top level: BBa_K1486001
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K1486000
type: Component
persistent identity: component2382078
display id: component2382078
version: 1
top level: BBa_K1486001
access: public
definition: BBa_K1486000

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2382074
display id: annotation2382074
version: 1
top level: BBa_K1486001
location: https://synbiohub.org/public/igem/BBa_K1486001/annotation2382074/range2382074/1
component: BBa_B0034

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type: Range
persistent identity: range2382074
display id: range2382074
version: 1
direction: direction/reverse
top level: BBa_K1486001
start: 1211
end: 1222
orientation: inline

BBa_K1486000
type: SequenceAnnotation
persistent identity: annotation2382078
display id: annotation2382078
version: 1
top level: BBa_K1486001
location: https://synbiohub.org/public/igem/BBa_K1486001/annotation2382078/range2382078/1
component: BBa_K1486000

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type: Range
persistent identity: range2382078
display id: range2382078
version: 1
direction: direction/reverse
top level: BBa_K1486001
start: 1223
end: 1924
orientation: inline

BBa_I0500
type: SequenceAnnotation
persistent identity: annotation2382072
display id: annotation2382072
version: 1
top level: BBa_K1486001
location: https://synbiohub.org/public/igem/BBa_K1486001/annotation2382072/range2382072/1
component: BBa_I0500

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type: Range
persistent identity: range2382072
display id: range2382072
version: 1
direction: direction/reverse
top level: BBa_K1486001
start: 1
end: 1210
orientation: inline

BBa_K2023012
type: ComponentDefinition
persistent identity: BBa K2023012
display id: BBa_K2023012
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Pr-RBS-XylR-His tag
creator: C??lia Chenebault
created: 2016-10-13T11:00:00Z
modified: 2016-10-20T04:44:22Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2490_
m user id: 30356
owner id: 30230
owning group id: 9
status: status/Planning
bookmark: false
mutable description: XylR-His tag-term
mutable notes: blabla
mutable provenance: IDT
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K2023010
component: BBa_B0034
component: BBa_I723018
sequence annotation: BBa_K2023010
sequence annotation: BBa_B0034
sequence annotation: BBa_I723018
sequence: https://synbiohub.org/public/igem/BBa_K2023012_sequence/1

BBa_K2023010
type: Component
persistent identity: component2520032
display id: component2520032
version: 1
top level: BBa_K2023012
access: public
definition: BBa_K2023010

BBa_B0034
type: Component
persistent identity: component2520027
display id: component2520027
version: 1
top level: BBa_K2023012
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_I723018
type: Component
persistent identity: component2520025
display id: component2520025
version: 1
top level: BBa_K2023012
access: public
definition: https://synbiohub.org/public/igem/BBa_I723018/1

BBa_K2023010
type: SequenceAnnotation
persistent identity: annotation2520032
display id: annotation2520032
version: 1
top level: BBa_K2023012
location: https://synbiohub.org/public/igem/BBa_K2023012/annotation2520032/range2520032/1
component: BBa_K2023010

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type: Range
persistent identity: range2520032
display id: range2520032
version: 1
direction: direction/reverse
top level: BBa_K2023012
start: 437
end: 2164
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2520027
display id: annotation2520027
version: 1
top level: BBa_K2023012
location: https://synbiohub.org/public/igem/BBa_K2023012/annotation2520027/range2520027/1
component: BBa_B0034

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type: Range
persistent identity: range2520027
display id: range2520027
version: 1
direction: direction/reverse
top level: BBa_K2023012
start: 419
end: 430
orientation: inline

BBa_I723018
type: SequenceAnnotation
persistent identity: annotation2520025
display id: annotation2520025
version: 1
top level: BBa_K2023012
location: https://synbiohub.org/public/igem/BBa_K2023012/annotation2520025/range2520025/1
component: BBa_I723018

https://synbiohub.org/public/igem/BBa_K2023012/annotation2520025/range2520025/1
type: Range
persistent identity: range2520025
display id: range2520025
version: 1
direction: direction/reverse
top level: BBa_K2023012
start: 1
end: 410
orientation: inline

BBa_K389412
type: ComponentDefinition
persistent identity: BBa K389412
display id: BBa_K389412
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: vir promoter + sensitivity tuner + luciferase
creator: Jonas Aretz
created: 2010-10-10T11:00:00Z
modified: 2015-05-08T01:12:19Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _517_
m user id: 0
owner id: 6670
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part combines a mutated virG response regulator from the VirA/G receptor system under the control of a constitutive promoter with a sensitivity tuner before a luciferase gene under the control of a vir promoter. The sensitivity tuner enhances a signal from a promoter so it enhances the readout luciferase.
mutable notes: sensitivity tuner to enhance the luciferase signal
mutable provenance: * luciferase from pGL4.10luc2 (Promega) * sensitivity tuner part, terminators and constitutive promoter from the partsregistry * ''virG'' was synthesized by Mr. Gene * ''vir'' promoter from ''Agrobacterium tumefaciens'' C58, TI-plasmid
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0017
component: BBa_K389002
component: BBa_K389003
component: BBa_J23110
component: BBa_K389402
sequence annotation: BBa_K389003
sequence annotation: BBa_B0017
sequence annotation: BBa_J23110
sequence annotation: BBa_K389002
sequence annotation: BBa_K389402
sequence: https://synbiohub.org/public/igem/BBa_K389412_sequence/1

BBa_B0017
type: Component
persistent identity: component2300770
display id: component2300770
version: 1
top level: BBa_K389412
access: public
definition: https://synbiohub.org/public/igem/BBa_B0017/1

BBa_K389002
type: Component
persistent identity: component2300765
display id: component2300765
version: 1
top level: BBa_K389412
access: public
definition: virG

BBa_K389003
type: Component
persistent identity: component2300775
display id: component2300775
version: 1
top level: BBa_K389412
access: public
definition: https://synbiohub.org/public/igem/BBa_K389003/1

BBa_J23110
type: Component
persistent identity: component2300761
display id: component2300761
version: 1
top level: BBa_K389412
access: public
definition: https://synbiohub.org/public/igem/BBa_J23110/1

BBa_K389402
type: Component
persistent identity: component2300799
display id: component2300799
version: 1
top level: BBa_K389412
access: public
definition: https://synbiohub.org/public/igem/BBa_K389402/1

BBa_K389003
type: SequenceAnnotation
persistent identity: annotation2300775
display id: annotation2300775
version: 1
top level: BBa_K389412
location: https://synbiohub.org/public/igem/BBa_K389412/annotation2300775/range2300775/1
component: BBa_K389003

https://synbiohub.org/public/igem/BBa_K389412/annotation2300775/range2300775/1
type: Range
persistent identity: range2300775
display id: range2300775
version: 1
direction: direction/reverse
top level: BBa_K389412
start: 968
end: 1125
orientation: inline

BBa_B0017
type: SequenceAnnotation
persistent identity: annotation2300770
display id: annotation2300770
version: 1
top level: BBa_K389412
location: https://synbiohub.org/public/igem/BBa_K389412/annotation2300770/range2300770/1
component: BBa_B0017

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type: Range
persistent identity: range2300770
display id: range2300770
version: 1
direction: direction/reverse
top level: BBa_K389412
start: 792
end: 959
orientation: inline

BBa_J23110
type: SequenceAnnotation
persistent identity: annotation2300761
display id: annotation2300761
version: 1
top level: BBa_K389412
location: https://synbiohub.org/public/igem/BBa_K389412/annotation2300761/range2300761/1
component: BBa_J23110

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type: Range
persistent identity: range2300761
display id: range2300761
version: 1
direction: direction/reverse
top level: BBa_K389412
start: 1
end: 35
orientation: inline

BBa_K389002
type: SequenceAnnotation
persistent identity: annotation2300765
display id: annotation2300765
version: 1
top level: BBa_K389412
location: https://synbiohub.org/public/igem/BBa_K389412/annotation2300765/range2300765/1
component: BBa_K389002

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type: Range
persistent identity: range2300765
display id: range2300765
version: 1
direction: direction/reverse
top level: BBa_K389412
start: 44
end: 783
orientation: inline

BBa_K389402
type: SequenceAnnotation
persistent identity: annotation2300799
display id: annotation2300799
version: 1
top level: BBa_K389412
location: https://synbiohub.org/public/igem/BBa_K389412/annotation2300799/range2300799/1
component: BBa_K389402

https://synbiohub.org/public/igem/BBa_K389412/annotation2300799/range2300799/1
type: Range
persistent identity: range2300799
display id: range2300799
version: 1
direction: direction/reverse
top level: BBa_K389412
start: 1134
end: 4020
orientation: inline

BBa_K1315000
type: ComponentDefinition
persistent identity: BBa K1315000
display id: BBa_K1315000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pqsR
creator: Dimitrios Michailidis
created: 2014-09-19T11:00:00Z
modified: 2015-05-08T01:09:48Z
discontinued: true
dominant: false
experience: experience/None
group u list: _1690_
m user id: 0
owner id: 21812
owning group id: 9
sample status: Discontinued
status: status/Deleted
bookmark: false
mutable description: The Pseydomonas quinolone signal (PQS) as well as its precursor 2-heptyl-4-quinolone (HHQ)are very important in mechanisms controlling virulence in Pseudomonas aeruginosa. P. aeruginosa has a key role in Cystic Fibrosis (CF). PQS can initiate signalling for various processes like virulence, motility, and biofilm formation. A good number of these processes are facilitated by PqsR, which is a transcriptional factor. An activated PqsR will go to the nucleus of the cells and activate the respective genes. http://www.ncbi.nlm.nih.gov/pubmed/21539583 http://www.ncbi.nlm.nih.gov/pubmed/?term=pqsr-dependent+and+pqsr-independent+regulation+of+motility+and+biofilm+formation+by+pqs http://www.ncbi.nlm.nih.gov/pubmed/12393198
mutable notes: The sequence did not have any restriction sites to remove so it was immediately PCRed out of the genome and had the registry prefix and suffix added to it. It was then ligated into the pSB1C3 vector.
mutable provenance: The source of this part is the genome of Pseudomonas aeruginosa PAO1 provided by the Robert Ryan lab in the Molecular Microbiology department of the University of Dundee, UK.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: PqsR
sequence: https://synbiohub.org/public/igem/BBa_K1315000_sequence/1

PqsR
type: SequenceAnnotation
persistent identity: annotation2384759
display id: annotation2384759
version: 1
top level: BBa_K1315000
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1315000/annotation2384759/range2384759/1

https://synbiohub.org/public/igem/BBa_K1315000/annotation2384759/range2384759/1
type: Range
persistent identity: range2384759
display id: range2384759
version: 1
direction: direction/forward
top level: BBa_K1315000
start: 1
end: 999
orientation: inline

BBa_K1946005
type: ComponentDefinition
persistent identity: BBa K1946005
display id: BBa_K1946005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: XylR
creator: Ayşe Naz Ozant??rk
created: 2016-10-12T11:00:00Z
modified: 2016-10-13T05:23:37Z
discontinued: false
dominant: false
group u list: _2413_
m user id: 29837
owner id: 29837
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part codes for the transcriptional regulatory protein XylR from Pseudomonas putida. It activates the TOL plasmid xyl operons in the presence of toluene, m-, p- and o-xylene.
mutable notes: The sequence was codon optimized for E.coli K12.
mutable provenance: The source translation sequence can be accessed at GenBank: AAB59162.1
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: XylR coding region
sequence annotation: stop codon
sequence annotation: start codon
sequence: https://synbiohub.org/public/igem/BBa_K1946005_sequence/1

XylR coding region
type: SequenceAnnotation
persistent identity: annotation2497253
display id: annotation2497253
version: 1
top level: BBa_K1946005
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1946005/annotation2497253/range2497253/1

https://synbiohub.org/public/igem/BBa_K1946005/annotation2497253/range2497253/1
type: Range
persistent identity: range2497253
display id: range2497253
version: 1
direction: direction/forward
top level: BBa_K1946005
start: 1
end: 1698
orientation: inline

stop codon
type: SequenceAnnotation
persistent identity: annotation2497256
display id: annotation2497256
version: 1
top level: BBa_K1946005
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1946005/annotation2497256/range2497256/1

https://synbiohub.org/public/igem/BBa_K1946005/annotation2497256/range2497256/1
type: Range
persistent identity: range2497256
display id: range2497256
version: 1
direction: direction/forward
top level: BBa_K1946005
start: 1699
end: 1701
orientation: inline

start codon
type: SequenceAnnotation
persistent identity: annotation2497252
display id: annotation2497252
version: 1
top level: BBa_K1946005
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1946005/annotation2497252/range2497252/1

https://synbiohub.org/public/igem/BBa_K1946005/annotation2497252/range2497252/1
type: Range
persistent identity: range2497252
display id: range2497252
version: 1
direction: direction/forward
top level: BBa_K1946005
start: 1
end: 3
orientation: inline

BBa_K1520023
type: ComponentDefinition
persistent identity: BBa K1520023
display id: BBa_K1520023
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Pcons2-rbs-lacI-Ter-Plac-rbs-luxI-Ter-Pcons2-rbs-luxR-Ter-Prlux-rbs-rfp-rbs-luxR-Ter
creator: yue wu
created: 2014-09-21T11:00:00Z
modified: 2015-05-08T01:10:49Z
discontinued: false
dominant: false
group u list: _1902_
m user id: 0
owner id: 21318
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Plasmid in positive feedback verification: Pcons2-rbs-lacI-Ter-Plac-rbs-luxI-Ter-Pcons2-rbs-luxR-Ter-Prlux-rbs-rfp-rbs-luxR-Ter. It is a positive feedback plasmid using luxR as key to feedback. Introducing IPTG will produce rfp and luxR, and luxR will work back to enhance production of rfp.
mutable notes: None
mutable provenance: Pcons2, lacI, Plac, luxI, luxR, Prlux, rfp, Ter
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1520020
component: BBa_K1520012
sequence annotation: BBa_K1520020
sequence annotation: BBa_K1520012
sequence: https://synbiohub.org/public/igem/BBa_K1520023_sequence/1

BBa_K1520020
type: Component
persistent identity: component2385213
display id: component2385213
version: 1
top level: BBa_K1520023
access: public
definition: BBa_K1520020

BBa_K1520012
type: Component
persistent identity: component2385224
display id: component2385224
version: 1
top level: BBa_K1520023
access: public
definition: BBa_K1520012

BBa_K1520020
type: SequenceAnnotation
persistent identity: annotation2385213
display id: annotation2385213
version: 1
top level: BBa_K1520023
location: https://synbiohub.org/public/igem/BBa_K1520023/annotation2385213/range2385213/1
component: BBa_K1520020

https://synbiohub.org/public/igem/BBa_K1520023/annotation2385213/range2385213/1
type: Range
persistent identity: range2385213
display id: range2385213
version: 1
direction: direction/reverse
top level: BBa_K1520023
start: 1
end: 3352
orientation: inline

BBa_K1520012
type: SequenceAnnotation
persistent identity: annotation2385224
display id: annotation2385224
version: 1
top level: BBa_K1520023
location: https://synbiohub.org/public/igem/BBa_K1520023/annotation2385224/range2385224/1
component: BBa_K1520012

https://synbiohub.org/public/igem/BBa_K1520023/annotation2385224/range2385224/1
type: Range
persistent identity: range2385224
display id: range2385224
version: 1
direction: direction/reverse
top level: BBa_K1520023
start: 3361
end: 5099
orientation: inline

VP64
type: ComponentDefinition
persistent identity: BBa J176013
display id: BBa_J176013
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: VP64
creator: Karmella Haynes
created: 2011-09-28T11:00:00Z
modified: 2015-08-31T04:08:33Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _863_
m user id: 0
owner id: 10865
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Tetrameric VP16 transc. activator domain
mutable notes: TBA
mutable provenance: TBA
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: VP16
sequence: https://synbiohub.org/public/igem/BBa_J176013_sequence/1

VP16
type: SequenceAnnotation
persistent identity: annotation2166131
display id: annotation2166131
version: 1
top level: VP64
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_J176013/annotation2166131/range2166131/1

https://synbiohub.org/public/igem/BBa_J176013/annotation2166131/range2166131/1
type: Range
persistent identity: range2166131
display id: range2166131
version: 1
direction: direction/forward
top level: VP64
start: 79
end: 111
orientation: inline

VP16
type: SequenceAnnotation
persistent identity: annotation2166130
display id: annotation2166130
version: 1
top level: VP64
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_J176013/annotation2166130/range2166130/1

https://synbiohub.org/public/igem/BBa_J176013/annotation2166130/range2166130/1
type: Range
persistent identity: range2166130
display id: range2166130
version: 1
direction: direction/forward
top level: VP64
start: 40
end: 72
orientation: inline

VP16
type: SequenceAnnotation
persistent identity: annotation2166132
display id: annotation2166132
version: 1
top level: VP64
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_J176013/annotation2166132/range2166132/1

https://synbiohub.org/public/igem/BBa_J176013/annotation2166132/range2166132/1
type: Range
persistent identity: range2166132
display id: range2166132
version: 1
direction: direction/forward
top level: VP64
start: 118
end: 150
orientation: inline

VP16
type: SequenceAnnotation
persistent identity: annotation2166129
display id: annotation2166129
version: 1
top level: VP64
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_J176013/annotation2166129/range2166129/1

https://synbiohub.org/public/igem/BBa_J176013/annotation2166129/range2166129/1
type: Range
persistent identity: range2166129
display id: range2166129
version: 1
direction: direction/forward
top level: VP64
start: 1
end: 33
orientation: inline

BBa_K783054
type: ComponentDefinition
persistent identity: BBa K783054
display id: BBa_K783054
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: This is a MoClo converted version of BBa_C0079
creator: Traci Haddock
created: 2012-10-01T11:00:00Z
modified: 2015-05-08T01:13:21Z
discontinued: false
dominant: false
group u list: _1038_
m user id: 0
owner id: 8248
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This is a MoClo converted version of BBa_C0079, which is the coding region for the lasR protein, which accepts chemical signal AI-1 (made by lasI protein). It has two MoClo fusion sites flanking it.
mutable notes: We had to design the proper MoClo fusion sites to flank this part.
mutable provenance: BBa_C0079
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: G
sequence annotation: LVA
sequence annotation: lasR
sequence: https://synbiohub.org/public/igem/BBa_K783054_sequence/1

G
type: SequenceAnnotation
persistent identity: annotation2214300
display id: annotation2214300
version: 1
top level: BBa_K783054
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K783054/annotation2214300/range2214300/1

https://synbiohub.org/public/igem/BBa_K783054/annotation2214300/range2214300/1
type: Range
persistent identity: range2214300
display id: range2214300
version: 1
direction: direction/forward
top level: BBa_K783054
start: 480
end: 480
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation2214301
display id: annotation2214301
version: 1
top level: BBa_K783054
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K783054/annotation2214301/range2214301/1

https://synbiohub.org/public/igem/BBa_K783054/annotation2214301/range2214301/1
type: Range
persistent identity: range2214301
display id: range2214301
version: 1
direction: direction/forward
top level: BBa_K783054
start: 718
end: 750
orientation: inline

lasR
type: SequenceAnnotation
persistent identity: annotation2214299
display id: annotation2214299
version: 1
top level: BBa_K783054
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K783054/annotation2214299/range2214299/1

https://synbiohub.org/public/igem/BBa_K783054/annotation2214299/range2214299/1
type: Range
persistent identity: range2214299
display id: range2214299
version: 1
direction: direction/forward
top level: BBa_K783054
start: 1
end: 717
orientation: inline

BBa_K1486005
type: ComponentDefinition
persistent identity: BBa K1486005
display id: BBa_K1486005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Arabinose promoter + sfGFP-CpxR [Cterm]
creator: iGem EPFL 2014
created: 2014-08-12T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part is composed of an arabinose-inducible promoter for superfolded GFP attached by a linker to CpxR (on the C terminus of CpxR).
mutable notes: We had to test both attachments, N and C terminus of the CpxR.
mutable provenance: The arabinose promoter and the sfGFP were obtained from the registry, while the CpxR was obtained from the E coli genome.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_I746916
component: BBa_B0034
component: BBa_I0500
component: BBa_K1486000
component: BBa_K1486003
sequence annotation: BBa_K1486003
sequence annotation: BBa_B0034
sequence annotation: BBa_I0500
sequence annotation: BBa_I746916
sequence annotation: BBa_K1486000
sequence: https://synbiohub.org/public/igem/BBa_K1486005_sequence/1

BBa_I746916
type: Component
persistent identity: component2382093
display id: component2382093
version: 1
top level: BBa_K1486005
access: public
definition: https://synbiohub.org/public/igem/BBa_I746916/1

BBa_B0034
type: Component
persistent identity: component2382084
display id: component2382084
version: 1
top level: BBa_K1486005
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_I0500
type: Component
persistent identity: component2382082
display id: component2382082
version: 1
top level: BBa_K1486005
access: public
definition: https://synbiohub.org/public/igem/BBa_I0500/1

BBa_K1486000
type: Component
persistent identity: component2382088
display id: component2382088
version: 1
top level: BBa_K1486005
access: public
definition: BBa_K1486000

BBa_K1486003
type: Component
persistent identity: component2382089
display id: component2382089
version: 1
top level: BBa_K1486005
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486003/1

BBa_K1486003
type: SequenceAnnotation
persistent identity: annotation2382089
display id: annotation2382089
version: 1
top level: BBa_K1486005
location: https://synbiohub.org/public/igem/BBa_K1486005/annotation2382089/range2382089/1
component: BBa_K1486003

https://synbiohub.org/public/igem/BBa_K1486005/annotation2382089/range2382089/1
type: Range
persistent identity: range2382089
display id: range2382089
version: 1
direction: direction/reverse
top level: BBa_K1486005
start: 1925
end: 1948
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2382084
display id: annotation2382084
version: 1
top level: BBa_K1486005
location: https://synbiohub.org/public/igem/BBa_K1486005/annotation2382084/range2382084/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1486005/annotation2382084/range2382084/1
type: Range
persistent identity: range2382084
display id: range2382084
version: 1
direction: direction/reverse
top level: BBa_K1486005
start: 1211
end: 1222
orientation: inline

BBa_I0500
type: SequenceAnnotation
persistent identity: annotation2382082
display id: annotation2382082
version: 1
top level: BBa_K1486005
location: https://synbiohub.org/public/igem/BBa_K1486005/annotation2382082/range2382082/1
component: BBa_I0500

https://synbiohub.org/public/igem/BBa_K1486005/annotation2382082/range2382082/1
type: Range
persistent identity: range2382082
display id: range2382082
version: 1
direction: direction/reverse
top level: BBa_K1486005
start: 1
end: 1210
orientation: inline

BBa_I746916
type: SequenceAnnotation
persistent identity: annotation2382093
display id: annotation2382093
version: 1
top level: BBa_K1486005
location: https://synbiohub.org/public/igem/BBa_K1486005/annotation2382093/range2382093/1
component: BBa_I746916

https://synbiohub.org/public/igem/BBa_K1486005/annotation2382093/range2382093/1
type: Range
persistent identity: range2382093
display id: range2382093
version: 1
direction: direction/reverse
top level: BBa_K1486005
start: 1949
end: 2668
orientation: inline

BBa_K1486000
type: SequenceAnnotation
persistent identity: annotation2382088
display id: annotation2382088
version: 1
top level: BBa_K1486005
location: https://synbiohub.org/public/igem/BBa_K1486005/annotation2382088/range2382088/1
component: BBa_K1486000

https://synbiohub.org/public/igem/BBa_K1486005/annotation2382088/range2382088/1
type: Range
persistent identity: range2382088
display id: range2382088
version: 1
direction: direction/reverse
top level: BBa_K1486005
start: 1223
end: 1924
orientation: inline

BBa_K1520013
type: ComponentDefinition
persistent identity: BBa K1520013
display id: BBa_K1520013
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Prlux-rbs-rfp-rbs-luxI-rbs-luxR-Ter
creator: yue wu
created: 2014-09-21T11:00:00Z
modified: 2015-05-08T01:10:49Z
discontinued: false
dominant: false
group u list: _1902_
m user id: 0
owner id: 21318
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Prlux-rbs-rfp-rbs-luxI-rbs-luxR-Ter. It produces rfp, luxI and luxR when there is enough luxR and AHL.
mutable notes: None
mutable provenance: Prlux, rfp, luxI, luxR, Ter
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1520029
component: BBa_K1520504
component: BBa_K1520501
component: BBa_R0062
component: BBa_K1520500
sequence annotation: BBa_K1520504
sequence annotation: BBa_K1520500
sequence annotation: BBa_K1520501
sequence annotation: BBa_K1520029
sequence annotation: BBa_R0062
sequence: https://synbiohub.org/public/igem/BBa_K1520013_sequence/1

BBa_K1520029
type: Component
persistent identity: component2384991
display id: component2384991
version: 1
top level: BBa_K1520013
access: public
definition: https://synbiohub.org/public/igem/BBa_K1520029/1

BBa_K1520504
type: Component
persistent identity: component2384987
display id: component2384987
version: 1
top level: BBa_K1520013
access: public
definition: https://synbiohub.org/public/igem/BBa_K1520504/1

BBa_K1520501
type: Component
persistent identity: component2384990
display id: component2384990
version: 1
top level: BBa_K1520013
access: public
definition: BBa_K1520501

BBa_R0062
type: Component
persistent identity: component2384979
display id: component2384979
version: 1
top level: BBa_K1520013
access: public
definition: https://synbiohub.org/public/igem/BBa_R0062/1

BBa_K1520500
type: Component
persistent identity: component2384984
display id: component2384984
version: 1
top level: BBa_K1520013
access: public
definition: https://synbiohub.org/public/igem/BBa_K1520500/1

BBa_K1520504
type: SequenceAnnotation
persistent identity: annotation2384987
display id: annotation2384987
version: 1
top level: BBa_K1520013
location: https://synbiohub.org/public/igem/BBa_K1520013/annotation2384987/range2384987/1
component: BBa_K1520504

https://synbiohub.org/public/igem/BBa_K1520013/annotation2384987/range2384987/1
type: Range
persistent identity: range2384987
display id: range2384987
version: 1
direction: direction/reverse
top level: BBa_K1520013
start: 804
end: 1464
orientation: inline

BBa_K1520500
type: SequenceAnnotation
persistent identity: annotation2384984
display id: annotation2384984
version: 1
top level: BBa_K1520013
location: https://synbiohub.org/public/igem/BBa_K1520013/annotation2384984/range2384984/1
component: BBa_K1520500

https://synbiohub.org/public/igem/BBa_K1520013/annotation2384984/range2384984/1
type: Range
persistent identity: range2384984
display id: range2384984
version: 1
direction: direction/reverse
top level: BBa_K1520013
start: 64
end: 795
orientation: inline

BBa_K1520501
type: SequenceAnnotation
persistent identity: annotation2384990
display id: annotation2384990
version: 1
top level: BBa_K1520013
location: https://synbiohub.org/public/igem/BBa_K1520013/annotation2384990/range2384990/1
component: BBa_K1520501

https://synbiohub.org/public/igem/BBa_K1520013/annotation2384990/range2384990/1
type: Range
persistent identity: range2384990
display id: range2384990
version: 1
direction: direction/reverse
top level: BBa_K1520013
start: 1473
end: 2271
orientation: inline

BBa_K1520029
type: SequenceAnnotation
persistent identity: annotation2384991
display id: annotation2384991
version: 1
top level: BBa_K1520013
location: https://synbiohub.org/public/igem/BBa_K1520013/annotation2384991/range2384991/1
component: BBa_K1520029

https://synbiohub.org/public/igem/BBa_K1520013/annotation2384991/range2384991/1
type: Range
persistent identity: range2384991
display id: range2384991
version: 1
direction: direction/reverse
top level: BBa_K1520013
start: 2280
end: 2408
orientation: inline

BBa_R0062
type: SequenceAnnotation
persistent identity: annotation2384979
display id: annotation2384979
version: 1
top level: BBa_K1520013
location: https://synbiohub.org/public/igem/BBa_K1520013/annotation2384979/range2384979/1
component: BBa_R0062

https://synbiohub.org/public/igem/BBa_K1520013/annotation2384979/range2384979/1
type: Range
persistent identity: range2384979
display id: range2384979
version: 1
direction: direction/forward
top level: BBa_K1520013
start: 1
end: 55
orientation: inline