//cds/transcriptionalregulator/repressor
type: Collection
persistent identity: cds transcriptionalregulator repressor
display id: cds_transcriptionalregulator_repressor
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
owned by: james
owned by: myers
member: BBa_K1520514
member: BBa_K544012
member: lacI
member: BBa_K1509000
member: BBa_K608351
member: BBa_K142006
member: BBa_K1520013
member: LacIM
member: BBa_K1520502
member: BBa_K374002
member: cI TP901
member: BBa_K1520004
member: BBa_K1520008
member: BBa_K1031510
member: BBa_K1031743
member: BBa_K106667
member: BBa_K1480003
member: BBa_K142002
member: BBa_K1682011
member: BBa_K1520017
member: ARL4A0604
member: BBa_K518003
member: BBa_K1031016
member: BBa_K108021
member: BBa_C2006
member: mnt (s)
member: lacI ts
member: BBa_K864202
member: BBa_K142003
member: BBa_K1520518
member: BBa_K1520505
member: BBa_K1520501
member: ARL2A0301
member: BBa_K1480005
member: BBa_K1720004
member: LacI
member: BBa_K106666
member: BBa_K1520021
member: BBa_K1520007
member: BBa_I715032
member: luxr
member: ARL2A0203
member: LacI_I12
member: c2 P22
member: BBa_K1520503
member: BBa_K1031015
member: BBa_K1520520
member: BBa_K950001
member: ARL2A0102
member: LRLa
member: BBa_K822004
member: BBa_K1520015
member: rhIR
member: BBa_K544003
member: BBa_K1031013
member: cI lam
member: BBa_K1520006
member: BBa_K1520519
member: BBa_K091121
member: BBa_K1031014
member: BBa_K142001
member: tetR
member: BBa_K1720003
member: BBa_K082004
member: BBa_K544001
member: mnt (w)
member: penI
member: BBa_K292005
member: Fur
member: lexA
member: BBa_K106668

BBa_K106667
type: ComponentDefinition
persistent identity: BBa K106667
display id: BBa_K106667
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Lac Repressor, AarI BD part
creator: Andrej Ondracka
created: 2008-10-24T11:00:00Z
modified: 2015-05-08T01:08:59Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _226_
m user id: 0
owner id: 3416
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: Lac repressor from E.coli
mutable notes: .
mutable provenance: .
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K106667_sequence/1

BBa_K1520024
type: ComponentDefinition
persistent identity: BBa K1520024
display id: BBa_K1520024
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Pcons2-rbs-lacI-Ter-Plac-rbs-luxI-Ter-Pcons2-rbs-luxR-Ter-Prlux-rbs-rfp-rbs-luxI-rbs-luxR-Ter
creator: yue wu
created: 2014-09-21T11:00:00Z
modified: 2015-05-08T01:10:49Z
discontinued: false
dominant: false
group u list: _1902_
m user id: 0
owner id: 21318
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Plasmid in positive feedback verification: Pcons2-rbs-lacI-Ter-Plac-rbs-luxI-Ter-Pcons2-rbs-luxR-Ter-Prlux-rbs-rfp-rbs-luxI-rbs-luxR-Ter It is a positive feedback plasmid using luxI and luxR as key to feedback. Introducing IPTG will produce rfp, luxI and luxR, and luxI together with luxR will work back to enhance production of rfp.
mutable notes: None
mutable provenance: Pcons2, lacI, Plac, luxI, luxR, Prlux, rfp, Ter
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1520013
component: BBa_K1520020
sequence annotation: BBa_K1520013
sequence annotation: BBa_K1520020
sequence: https://synbiohub.org/public/igem/BBa_K1520024_sequence/1

BBa_K1520013
type: Component
persistent identity: component2385264
display id: component2385264
version: 1
top level: BBa_K1520024
access: public
definition: BBa_K1520013

BBa_K1520020
type: Component
persistent identity: component2385250
display id: component2385250
version: 1
top level: BBa_K1520024
access: public
definition: BBa_K1520020

BBa_K1520013
type: SequenceAnnotation
persistent identity: annotation2385264
display id: annotation2385264
version: 1
top level: BBa_K1520024
location: https://synbiohub.org/public/igem/BBa_K1520024/annotation2385264/range2385264/1
component: BBa_K1520013

https://synbiohub.org/public/igem/BBa_K1520024/annotation2385264/range2385264/1
type: Range
persistent identity: range2385264
display id: range2385264
version: 1
direction: direction/reverse
top level: BBa_K1520024
start: 3361
end: 5768
orientation: inline

BBa_K1520020
type: SequenceAnnotation
persistent identity: annotation2385250
display id: annotation2385250
version: 1
top level: BBa_K1520024
location: https://synbiohub.org/public/igem/BBa_K1520024/annotation2385250/range2385250/1
component: BBa_K1520020

https://synbiohub.org/public/igem/BBa_K1520024/annotation2385250/range2385250/1
type: Range
persistent identity: range2385250
display id: range2385250
version: 1
direction: direction/reverse
top level: BBa_K1520024
start: 1
end: 3352
orientation: inline

BBa_K1520518
type: ComponentDefinition
persistent identity: BBa K1520518
display id: BBa_K1520518
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PgolTS-golS-PgolB-tetR-Ter
creator: yue wu
created: 2014-09-22T11:00:00Z
modified: 2015-05-08T01:10:49Z
discontinued: false
dominant: false
group u list: _1902_
m user id: 0
owner id: 21318
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: PgolTS-golS-PgolB-tetR-Ter If there exist gold ions, tetR will be produced.
mutable notes: None
mutable provenance: PgolTS-golS-PgolB, tetR, Ter
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1520506
component: BBa_K1520029
component: BBa_K1520505
sequence annotation: BBa_K1520506
sequence annotation: BBa_K1520505
sequence annotation: BBa_K1520029
sequence: https://synbiohub.org/public/igem/BBa_K1520518_sequence/1

BBa_K1520506
type: Component
persistent identity: component2385739
display id: component2385739
version: 1
top level: BBa_K1520518
access: public
definition: https://synbiohub.org/public/igem/BBa_K1520506/1

BBa_K1520029
type: Component
persistent identity: component2385743
display id: component2385743
version: 1
top level: BBa_K1520518
access: public
definition: https://synbiohub.org/public/igem/BBa_K1520029/1

BBa_K1520505
type: Component
persistent identity: component2385742
display id: component2385742
version: 1
top level: BBa_K1520518
access: public
definition: BBa_K1520505

BBa_K1520506
type: SequenceAnnotation
persistent identity: annotation2385739
display id: annotation2385739
version: 1
top level: BBa_K1520518
location: https://synbiohub.org/public/igem/BBa_K1520518/annotation2385739/range2385739/1
component: BBa_K1520506

https://synbiohub.org/public/igem/BBa_K1520518/annotation2385739/range2385739/1
type: Range
persistent identity: range2385739
display id: range2385739
version: 1
direction: direction/reverse
top level: BBa_K1520518
start: 1
end: 753
orientation: inline

BBa_K1520505
type: SequenceAnnotation
persistent identity: annotation2385742
display id: annotation2385742
version: 1
top level: BBa_K1520518
location: https://synbiohub.org/public/igem/BBa_K1520518/annotation2385742/range2385742/1
component: BBa_K1520505

https://synbiohub.org/public/igem/BBa_K1520518/annotation2385742/range2385742/1
type: Range
persistent identity: range2385742
display id: range2385742
version: 1
direction: direction/reverse
top level: BBa_K1520518
start: 762
end: 1464
orientation: inline

BBa_K1520029
type: SequenceAnnotation
persistent identity: annotation2385743
display id: annotation2385743
version: 1
top level: BBa_K1520518
location: https://synbiohub.org/public/igem/BBa_K1520518/annotation2385743/range2385743/1
component: BBa_K1520029

https://synbiohub.org/public/igem/BBa_K1520518/annotation2385743/range2385743/1
type: Range
persistent identity: range2385743
display id: range2385743
version: 1
direction: direction/reverse
top level: BBa_K1520518
start: 1473
end: 1601
orientation: inline

BBa_K1520016
type: ComponentDefinition
persistent identity: BBa K1520016
display id: BBa_K1520016
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Pcons2-rbs-luxR-Ter-Prlux-rbs-lacI-Ter
creator: yue wu
created: 2014-09-21T11:00:00Z
modified: 2015-05-08T01:10:49Z
discontinued: false
dominant: false
group u list: _1902_
m user id: 0
owner id: 21318
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Pcons2-rbs-luxR-Ter-Prlux-rbs-lacI-Ter. It produces lacI when there is enough AHL.
mutable notes: None
mutable provenance: Pcons2, luxR, Prlux, lacI, Ter
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1520004
component: BBa_K1520003
sequence annotation: BBa_K1520004
sequence annotation: BBa_K1520003
sequence: https://synbiohub.org/public/igem/BBa_K1520016_sequence/1

BBa_K1520004
type: Component
persistent identity: component2385025
display id: component2385025
version: 1
top level: BBa_K1520016
access: public
definition: BBa_K1520004

BBa_K1520003
type: Component
persistent identity: component2385015
display id: component2385015
version: 1
top level: BBa_K1520016
access: public
definition: BBa_K1520003

BBa_K1520004
type: SequenceAnnotation
persistent identity: annotation2385025
display id: annotation2385025
version: 1
top level: BBa_K1520016
location: https://synbiohub.org/public/igem/BBa_K1520016/annotation2385025/range2385025/1
component: BBa_K1520004

https://synbiohub.org/public/igem/BBa_K1520016/annotation2385025/range2385025/1
type: Range
persistent identity: range2385025
display id: range2385025
version: 1
direction: direction/reverse
top level: BBa_K1520016
start: 988
end: 2358
orientation: inline

BBa_K1520003
type: SequenceAnnotation
persistent identity: annotation2385015
display id: annotation2385015
version: 1
top level: BBa_K1520016
location: https://synbiohub.org/public/igem/BBa_K1520016/annotation2385015/range2385015/1
component: BBa_K1520003

https://synbiohub.org/public/igem/BBa_K1520016/annotation2385015/range2385015/1
type: Range
persistent identity: range2385015
display id: range2385015
version: 1
direction: direction/reverse
top level: BBa_K1520016
start: 1
end: 979
orientation: inline

rhlR
type: ComponentDefinition
persistent identity: BBa C0071
display id: BBa_C0071
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: rhlR repressor/activator from P. aeruginosa PA3477 (+LVA)
creator: Debra Lin, Srini Devadas, David Gray, Ronny Krashinsky, and Chris Zheng Liu, Boopers
created: 2004-01-26T12:00:00Z
modified: 2015-08-31T04:07:23Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Transcriptional regulator, in complex with N-butyryl-HSL, RhlR binds to the Rhl promoter
mutable notes: (1/28/04 Edit Jamboree) (0) No BB sites (1) ATG (2) TAATAA (3) Blast checks out (4) No codon changes (5) Maybe chassis compatability (check by experiment) (6) Codon usage not optimal (but looks OK) (7) ssrA tag added
mutable provenance: P. aeruginosa PA3477
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: rhlR
sequence annotation: LVA
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C0071_sequence/1

rhlR
type: SequenceAnnotation
persistent identity: annotation301192
display id: annotation301192
version: 1
top level: rhlR
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_C0071/annotation301192/range301192/1

https://synbiohub.org/public/igem/BBa_C0071/annotation301192/range301192/1
type: Range
persistent identity: range301192
display id: range301192
version: 1
direction: direction/forward
top level: rhlR
start: 1
end: 723
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation306523
display id: annotation306523
version: 1
top level: rhlR
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_C0071/annotation306523/range306523/1

https://synbiohub.org/public/igem/BBa_C0071/annotation306523/range306523/1
type: Range
persistent identity: range306523
display id: range306523
version: 1
direction: direction/forward
top level: rhlR
start: 724
end: 756
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213995
display id: annotation2213995
version: 1
top level: rhlR
role: SO:0000807
role: feature/barcode
location: https://synbiohub.org/public/igem/BBa_C0071/annotation2213995/range2213995/1

https://synbiohub.org/public/igem/BBa_C0071/annotation2213995/range2213995/1
type: Range
persistent identity: range2213995
display id: range2213995
version: 1
direction: direction/forward
top level: rhlR
start: 763
end: 787
orientation: inline

BBa_K950009
type: ComponentDefinition
persistent identity: BBa K950009
display id: BBa_K950009
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: yeast mig1
creator: Jakob Matthes
created: 2012-09-12T11:00:00Z
modified: 2015-05-08T01:13:49Z
discontinued: false
dominant: false
group u list: _1218_
m user id: 0
owner id: 11576
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: mig1 is a repressor targeting the promotor of suc2.
mutable notes: original sequence was not altered
mutable provenance: Sequence and information is based on the Saccharomyces Genome Database, see http://www.yeastgenome.org/cgi-bin/locus.fpl?locus=mig1
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: mig1
sequence: https://synbiohub.org/public/igem/BBa_K950009_sequence/1

mig1
type: SequenceAnnotation
persistent identity: annotation2182740
display id: annotation2182740
version: 1
top level: BBa_K950009
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K950009/annotation2182740/range2182740/1

https://synbiohub.org/public/igem/BBa_K950009/annotation2182740/range2182740/1
type: Range
persistent identity: range2182740
display id: range2182740
version: 1
direction: direction/forward
top level: BBa_K950009
start: 1
end: 1515
orientation: inline

BBa_K1861040
type: ComponentDefinition
persistent identity: BBa K1861040
display id: BBa_K1861040
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Fusion protein of SpyCatcher and RPD3 in an expression cassette
creator: Vladyslav Vyshnevskyi
created: 2015-09-17T11:00:00Z
modified: 2015-09-23T04:21:28Z
discontinued: false
dominant: false
group u list: _2291_
m user id: 25348
owner id: 25348
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Coming soon.
mutable notes: Coming soon.
mutable provenance: Coming soon.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1861104
component: BBa_K1861102
component: BBa_K392003
component: BBa_K1861401
component: BBa_K1861101
component: BBa_K1159200
sequence annotation: BBa_K1861101
sequence annotation: BBa_K1861401
sequence annotation: BBa_K392003
sequence annotation: BBa_K1861104
sequence annotation: BBa_K1159200
sequence annotation: BBa_K1861102
sequence: https://synbiohub.org/public/igem/BBa_K1861040_sequence/1

BBa_K1861104
type: Component
persistent identity: component2474408
display id: component2474408
version: 1
top level: BBa_K1861040
access: public
definition: https://synbiohub.org/public/igem/BBa_K1861104/1

BBa_K1861102
type: Component
persistent identity: component2474401
display id: component2474401
version: 1
top level: BBa_K1861040
access: public
definition: https://synbiohub.org/public/igem/BBa_K1861102/1

BBa_K392003
type: Component
persistent identity: component2474409
display id: component2474409
version: 1
top level: BBa_K1861040
access: public
definition: https://synbiohub.org/public/igem/BBa_K392003/1

BBa_K1861401
type: Component
persistent identity: component2474407
display id: component2474407
version: 1
top level: BBa_K1861040
access: public
definition: https://synbiohub.org/public/igem/BBa_K1861401/1

BBa_K1861101
type: Component
persistent identity: component2474400
display id: component2474400
version: 1
top level: BBa_K1861040
access: public
definition: https://synbiohub.org/public/igem/BBa_K1861101/1

BBa_K1159200
type: Component
persistent identity: component2474406
display id: component2474406
version: 1
top level: BBa_K1861040
access: public
definition: https://synbiohub.org/public/igem/BBa_K1159200/1

BBa_K1861101
type: SequenceAnnotation
persistent identity: annotation2474400
display id: annotation2474400
version: 1
top level: BBa_K1861040
location: https://synbiohub.org/public/igem/BBa_K1861040/annotation2474400/range2474400/1
component: BBa_K1861101

https://synbiohub.org/public/igem/BBa_K1861040/annotation2474400/range2474400/1
type: Range
persistent identity: range2474400
display id: range2474400
version: 1
direction: direction/reverse
top level: BBa_K1861040
start: 1
end: 238
orientation: inline

BBa_K1861401
type: SequenceAnnotation
persistent identity: annotation2474407
display id: annotation2474407
version: 1
top level: BBa_K1861040
location: https://synbiohub.org/public/igem/BBa_K1861040/annotation2474407/range2474407/1
component: BBa_K1861401

https://synbiohub.org/public/igem/BBa_K1861040/annotation2474407/range2474407/1
type: Range
persistent identity: range2474407
display id: range2474407
version: 1
direction: direction/reverse
top level: BBa_K1861040
start: 605
end: 1954
orientation: inline

BBa_K392003
type: SequenceAnnotation
persistent identity: annotation2474409
display id: annotation2474409
version: 1
top level: BBa_K1861040
location: https://synbiohub.org/public/igem/BBa_K1861040/annotation2474409/range2474409/1
component: BBa_K392003

https://synbiohub.org/public/igem/BBa_K1861040/annotation2474409/range2474409/1
type: Range
persistent identity: range2474409
display id: range2474409
version: 1
direction: direction/reverse
top level: BBa_K1861040
start: 1964
end: 2092
orientation: inline

BBa_K1861104
type: SequenceAnnotation
persistent identity: annotation2474408
display id: annotation2474408
version: 1
top level: BBa_K1861040
location: https://synbiohub.org/public/igem/BBa_K1861040/annotation2474408/range2474408/1
component: BBa_K1861104

https://synbiohub.org/public/igem/BBa_K1861040/annotation2474408/range2474408/1
type: Range
persistent identity: range2474408
display id: range2474408
version: 1
direction: direction/reverse
top level: BBa_K1861040
start: 1955
end: 1963
orientation: inline

BBa_K1159200
type: SequenceAnnotation
persistent identity: annotation2474406
display id: annotation2474406
version: 1
top level: BBa_K1861040
location: https://synbiohub.org/public/igem/BBa_K1861040/annotation2474406/range2474406/1
component: BBa_K1159200

https://synbiohub.org/public/igem/BBa_K1861040/annotation2474406/range2474406/1
type: Range
persistent identity: range2474406
display id: range2474406
version: 1
direction: direction/reverse
top level: BBa_K1861040
start: 266
end: 604
orientation: inline

BBa_K1861102
type: SequenceAnnotation
persistent identity: annotation2474401
display id: annotation2474401
version: 1
top level: BBa_K1861040
location: https://synbiohub.org/public/igem/BBa_K1861040/annotation2474401/range2474401/1
component: BBa_K1861102

https://synbiohub.org/public/igem/BBa_K1861040/annotation2474401/range2474401/1
type: Range
persistent identity: range2474401
display id: range2474401
version: 1
direction: direction/reverse
top level: BBa_K1861040
start: 239
end: 265
orientation: inline

BBa_K1031742
type: ComponentDefinition
persistent identity: BBa K1031742
display id: BBa_K1031742
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: J23109-PaaX
creator: He Shuaixin
created: 2013-09-08T11:00:00Z
modified: 2015-05-08T01:08:47Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1338_
m user id: 0
owner id: 15843
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: 109-PaaX-TT
mutable notes: molecular cloning
mutable provenance: PCR genome E.coli
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: PaaX
sequence annotation: BBa_J23109
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K1031742_sequence/1

PaaX
type: SequenceAnnotation
persistent identity: annotation2357489
display id: annotation2357489
version: 1
top level: BBa_K1031742
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1031742/annotation2357489/range2357489/1

https://synbiohub.org/public/igem/BBa_K1031742/annotation2357489/range2357489/1
type: Range
persistent identity: range2357489
display id: range2357489
version: 1
direction: direction/forward
top level: BBa_K1031742
start: 62
end: 1012
orientation: inline

BBa_J23109
type: SequenceAnnotation
persistent identity: annotation2357488
display id: annotation2357488
version: 1
top level: BBa_K1031742
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1031742/annotation2357488/range2357488/1

https://synbiohub.org/public/igem/BBa_K1031742/annotation2357488/range2357488/1
type: Range
persistent identity: range2357488
display id: range2357488
version: 1
direction: direction/forward
top level: BBa_K1031742
start: 1
end: 35
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2357490
display id: annotation2357490
version: 1
top level: BBa_K1031742
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1031742/annotation2357490/range2357490/1

https://synbiohub.org/public/igem/BBa_K1031742/annotation2357490/range2357490/1
type: Range
persistent identity: range2357490
display id: range2357490
version: 1
direction: direction/forward
top level: BBa_K1031742
start: 44
end: 55
orientation: inline

BBa_C2006
type: ComponentDefinition
persistent identity: BBa C2006
display id: BBa_C2006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: MalE.FactorXa.Zif268-GCN4
creator: Reshma Shetty
created: 2007-03-27T11:00:00Z
modified: 2015-08-31T04:07:25Z
discontinued: false
dominant: false
group u list: _41_
m user id: 0
owner id: 126
owning group id: 70
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Maltose binding protein fused to Zif268-GCN4.
mutable notes: None.
mutable provenance: From pMAL-c2x and BBa_C2005.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_C2006_sequence/1

BBa_K1520520
type: ComponentDefinition
persistent identity: BBa K1520520
display id: BBa_K1520520
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PgolTS-golS-PgolB-tetR-Ter-PtetO-rfp-Ter-Plac-tetR-Ter
creator: yue wu
created: 2014-09-25T11:00:00Z
modified: 2015-05-08T01:10:49Z
discontinued: false
dominant: false
group u list: _1902_
m user id: 0
owner id: 21318
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: PgolTS-golS-PgolB-tetR-Ter-PtetO-rfp-Ter-Plac-tetR-Ter It is part of our test of suicide circuit by gold ions.
mutable notes: None
mutable provenance: PgolTS-golS-PgolB, tetR, PtetO, rfp, Plac, Ter
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1520014
component: BBa_K1520015
component: BBa_K1520518
sequence annotation: BBa_K1520015
sequence annotation: BBa_K1520014
sequence annotation: BBa_K1520518
sequence: https://synbiohub.org/public/igem/BBa_K1520520_sequence/1

BBa_K1520014
type: Component
persistent identity: component2386979
display id: component2386979
version: 1
top level: BBa_K1520520
access: public
definition: https://synbiohub.org/public/igem/BBa_K1520014/1

BBa_K1520015
type: Component
persistent identity: component2386991
display id: component2386991
version: 1
top level: BBa_K1520520
access: public
definition: BBa_K1520015

BBa_K1520518
type: Component
persistent identity: component2386971
display id: component2386971
version: 1
top level: BBa_K1520520
access: public
definition: BBa_K1520518

BBa_K1520015
type: SequenceAnnotation
persistent identity: annotation2386991
display id: annotation2386991
version: 1
top level: BBa_K1520520
location: https://synbiohub.org/public/igem/BBa_K1520520/annotation2386991/range2386991/1
component: BBa_K1520015

https://synbiohub.org/public/igem/BBa_K1520520/annotation2386991/range2386991/1
type: Range
persistent identity: range2386991
display id: range2386991
version: 1
direction: direction/reverse
top level: BBa_K1520520
start: 2549
end: 3596
orientation: inline

BBa_K1520014
type: SequenceAnnotation
persistent identity: annotation2386979
display id: annotation2386979
version: 1
top level: BBa_K1520520
location: https://synbiohub.org/public/igem/BBa_K1520520/annotation2386979/range2386979/1
component: BBa_K1520014

https://synbiohub.org/public/igem/BBa_K1520520/annotation2386979/range2386979/1
type: Range
persistent identity: range2386979
display id: range2386979
version: 1
direction: direction/reverse
top level: BBa_K1520520
start: 1610
end: 2540
orientation: inline

BBa_K1520518
type: SequenceAnnotation
persistent identity: annotation2386971
display id: annotation2386971
version: 1
top level: BBa_K1520520
location: https://synbiohub.org/public/igem/BBa_K1520520/annotation2386971/range2386971/1
component: BBa_K1520518

https://synbiohub.org/public/igem/BBa_K1520520/annotation2386971/range2386971/1
type: Range
persistent identity: range2386971
display id: range2386971
version: 1
direction: direction/reverse
top level: BBa_K1520520
start: 1
end: 1601
orientation: inline

BBa_K105002
type: ComponentDefinition
persistent identity: BBa K105002
display id: BBa_K105002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TUP1 - repressor domain for transcription factors in yeast
creator: Sandra Dittmann
created: 2008-10-11T11:00:00Z
modified: 2015-05-08T01:08:52Z
discontinued: false
dominant: false
experience: experience/None
group u list: _253_
m user id: 0
owner id: 3313
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This BioBrick is the N-terminal repression domain of the yeast general repressor TUP1.<br\n> In artificial systems TUP1 is used in fusion with a DNA-binding domain. This DNA-binding domain is specific for a bait sequence. So the selective repressionn of genes with a promoter containing this bait sequence can be achieved.
mutable notes: To enable the fusion with other domains in frame the vector of this BioBrick has no base pair in between the restriction side and the BioBrick.<br\n> For more information about this issus, see Phillips, I.E. and Silver, P.A. "A new Biobrick Assembly Strategy Designed for Facile Protein Engineering." - DSpace http://hdl.handle.net/1721.1/32535 (2006).
mutable provenance: Yeast genomic DNA.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence: https://synbiohub.org/public/igem/BBa_K105002_sequence/1

ARL2A0101
type: ComponentDefinition
persistent identity: BBa I732105
display id: BBa_I732105
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: ARL2A0101
creator: Zhan Jian
created: 2007-10-13T11:00:00Z
modified: 2015-08-31T04:07:57Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_I732105_sequence/1

BBa_K1520012
type: ComponentDefinition
persistent identity: BBa K1520012
display id: BBa_K1520012
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Prlux-rbs-rfp-rbs-luxR-Ter
creator: yue wu
created: 2014-09-21T11:00:00Z
modified: 2015-05-08T01:10:49Z
discontinued: false
dominant: false
group u list: _1902_
m user id: 0
owner id: 21318
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Prlux-rbs-rfp-rbs-luxR-Ter. It produces rfp and luxR when there is enough rfp and luxR.
mutable notes: None
mutable provenance: Prlux, rfp, luxR, Ter
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1520501
component: BBa_K1520500
component: BBa_R0062
component: BBa_K1520029
sequence annotation: BBa_K1520500
sequence annotation: BBa_R0062
sequence annotation: BBa_K1520501
sequence annotation: BBa_K1520029
sequence: https://synbiohub.org/public/igem/BBa_K1520012_sequence/1

BBa_K1520501
type: Component
persistent identity: component2384976
display id: component2384976
version: 1
top level: BBa_K1520012
access: public
definition: BBa_K1520501

BBa_K1520500
type: Component
persistent identity: component2384973
display id: component2384973
version: 1
top level: BBa_K1520012
access: public
definition: https://synbiohub.org/public/igem/BBa_K1520500/1

BBa_R0062
type: Component
persistent identity: component2384968
display id: component2384968
version: 1
top level: BBa_K1520012
access: public
definition: https://synbiohub.org/public/igem/BBa_R0062/1

BBa_K1520029
type: Component
persistent identity: component2384977
display id: component2384977
version: 1
top level: BBa_K1520012
access: public
definition: https://synbiohub.org/public/igem/BBa_K1520029/1

BBa_K1520500
type: SequenceAnnotation
persistent identity: annotation2384973
display id: annotation2384973
version: 1
top level: BBa_K1520012
location: https://synbiohub.org/public/igem/BBa_K1520012/annotation2384973/range2384973/1
component: BBa_K1520500

https://synbiohub.org/public/igem/BBa_K1520012/annotation2384973/range2384973/1
type: Range
persistent identity: range2384973
display id: range2384973
version: 1
direction: direction/reverse
top level: BBa_K1520012
start: 64
end: 795
orientation: inline

BBa_R0062
type: SequenceAnnotation
persistent identity: annotation2384968
display id: annotation2384968
version: 1
top level: BBa_K1520012
location: https://synbiohub.org/public/igem/BBa_K1520012/annotation2384968/range2384968/1
component: BBa_R0062

https://synbiohub.org/public/igem/BBa_K1520012/annotation2384968/range2384968/1
type: Range
persistent identity: range2384968
display id: range2384968
version: 1
direction: direction/forward
top level: BBa_K1520012
start: 1
end: 55
orientation: inline

BBa_K1520501
type: SequenceAnnotation
persistent identity: annotation2384976
display id: annotation2384976
version: 1
top level: BBa_K1520012
location: https://synbiohub.org/public/igem/BBa_K1520012/annotation2384976/range2384976/1
component: BBa_K1520501

https://synbiohub.org/public/igem/BBa_K1520012/annotation2384976/range2384976/1
type: Range
persistent identity: range2384976
display id: range2384976
version: 1
direction: direction/reverse
top level: BBa_K1520012
start: 804
end: 1602
orientation: inline

BBa_K1520029
type: SequenceAnnotation
persistent identity: annotation2384977
display id: annotation2384977
version: 1
top level: BBa_K1520012
location: https://synbiohub.org/public/igem/BBa_K1520012/annotation2384977/range2384977/1
component: BBa_K1520029

https://synbiohub.org/public/igem/BBa_K1520012/annotation2384977/range2384977/1
type: Range
persistent identity: range2384977
display id: range2384977
version: 1
direction: direction/reverse
top level: BBa_K1520012
start: 1611
end: 1739
orientation: inline

rhIR
type: ComponentDefinition
persistent identity: BBa C0171
display id: BBa_C0171
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: rhlR repressor/activator from P. aeruginosa PA3477 (no LVA)
creator: jcbraff
created: 2004-05-26T11:00:00Z
modified: 2015-08-31T04:07:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_1_
m user id: 0
owner id: 61
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: same as C0071 except no LVA tag
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_C0171_sequence/1

ARL2A0203
type: ComponentDefinition
persistent identity: BBa I732110
display id: BBa_I732110
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: ARL2A0203
creator: Zhan Jian
created: 2007-10-13T11:00:00Z
modified: 2015-08-31T04:07:57Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_I732110_sequence/1

BBa_K106666
type: ComponentDefinition
persistent identity: BBa K106666
display id: BBa_K106666
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Lac Repressor, AarI AB part
creator: Andrej Ondracka
created: 2008-10-24T11:00:00Z
modified: 2015-05-08T01:08:59Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _226_
m user id: 0
owner id: 3416
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: Lac repressor from E.coli
mutable notes: .
mutable provenance: .
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K106666_sequence/1

lexA
type: ComponentDefinition
persistent identity: BBa K864201
display id: BBa_K864201
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LexA SOS response repressor
creator: Erik Lundin
created: 2012-09-24T11:00:00Z
modified: 2015-05-08T01:13:37Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1124_
m user id: 0
owner id: 9827
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: lexA is important in induction of recA
mutable notes: None
mutable provenance: Amplified from MG1655 (accession NC_000913) chromosome using primers listed below (annealing sequences are underlined) lexA_BB_F TTCGAATTCGCGGCCGCTTCTAGATGAAAGCGTTAACGGCCAG lexA_BB_R CGGCCGCTACTAGTATTATTACAGCCAGTCGCCGTT
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: lexA
sequence: https://synbiohub.org/public/igem/BBa_K864201_sequence/1

lexA
type: SequenceAnnotation
persistent identity: annotation2197113
display id: annotation2197113
version: 1
top level: lexA
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K864201/annotation2197113/range2197113/1

https://synbiohub.org/public/igem/BBa_K864201/annotation2197113/range2197113/1
type: Range
persistent identity: range2197113
display id: range2197113
version: 1
direction: direction/forward
top level: lexA
start: 1
end: 612
orientation: inline

BBa_K292005
type: ComponentDefinition
persistent identity: BBa K292005
display id: BBa_K292005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LacI repressor and a double terminator
creator: David Charoy
created: 2009-10-18T11:00:00Z
modified: 2015-05-08T01:11:49Z
discontinued: false
dominant: false
group u list: _394_
m user id: 0
owner id: 5212
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part contains a LacI repressor and a terminator (BBa_C0012 + BBa_B0014). The LacI repressor repressed pLac promoter from the E.coli lactose operon. So LacI repressor is really reliable to use when we want to control a gene expression by using pLac promoter. In fact when LacI is not expressed, pLac is activated, and when there is an expression of LacI, pLac is inhibited. We can easily design a system to induce the transcription of LacI and creates a failed feedback mechanism for pLac inhibition. History: In E.Coli, pLac is a part of the lactose operon. LacI is constantly expressed and represses the pLac. In the presence of Lactose, LacI is inhibited and pLac is active and allows the transcription of beta-galactosidase. Lactose can be substituted by IPTG (Isopropyl β-D-1-thiogalactopyranoside).
mutable notes: When we design the bio-brick it was impossible to obtain a functional bio-brick by using the plasmid SB1AK3. But it's worked with a pSB1A2.
mutable provenance: Part:BBa_C0012, Designed by Grace Kenney, Daniel Shen, Neelaksh Varshney, Samantha Sutton Group: Registry Part:BBa_B0014, Designed by Reshma Shetty Group: Registry (2003-07-16).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_C0012
component: BBa_B0014
sequence annotation: BBa_B0014
sequence annotation: BBa_C0012
sequence: https://synbiohub.org/public/igem/BBa_K292005_sequence/1

BBa_C0012
type: Component
persistent identity: component2246883
display id: component2246883
version: 1
top level: BBa_K292005
access: public
definition: lacI

BBa_B0014
type: Component
persistent identity: component2246893
display id: component2246893
version: 1
top level: BBa_K292005
access: public
definition: https://synbiohub.org/public/igem/BBa_B0014/1

BBa_B0014
type: SequenceAnnotation
persistent identity: annotation2246893
display id: annotation2246893
version: 1
top level: BBa_K292005
location: https://synbiohub.org/public/igem/BBa_K292005/annotation2246893/range2246893/1
component: BBa_B0014

https://synbiohub.org/public/igem/BBa_K292005/annotation2246893/range2246893/1
type: Range
persistent identity: range2246893
display id: range2246893
version: 1
direction: direction/reverse
top level: BBa_K292005
start: 1162
end: 1256
orientation: inline

BBa_C0012
type: SequenceAnnotation
persistent identity: annotation2246883
display id: annotation2246883
version: 1
top level: BBa_K292005
location: https://synbiohub.org/public/igem/BBa_K292005/annotation2246883/range2246883/1
component: BBa_C0012

https://synbiohub.org/public/igem/BBa_K292005/annotation2246883/range2246883/1
type: Range
persistent identity: range2246883
display id: range2246883
version: 1
direction: direction/forward
top level: BBa_K292005
start: 1
end: 1128
orientation: inline

BBa_K1031014
type: ComponentDefinition
persistent identity: BBa K1031014
display id: BBa_K1031014
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: NahR-Psal-B0031-RiboJ-CI
creator: He Shuaixin
created: 2013-09-08T11:00:00Z
modified: 2015-05-08T01:08:47Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1338_
m user id: 0
owner id: 15843
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: NahR Psel Ribo_J CI B0031
mutable notes: Pseudomonas putida, phiR73, E.coli
mutable provenance: lab made
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: promoter
sequence annotation: nahR
sequence annotation: cI
sequence annotation: b0031
sequence: https://synbiohub.org/public/igem/BBa_K1031014_sequence/1

promoter
type: SequenceAnnotation
persistent identity: annotation2354022
display id: annotation2354022
version: 1
top level: BBa_K1031014
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1031014/annotation2354022/range2354022/1

https://synbiohub.org/public/igem/BBa_K1031014/annotation2354022/range2354022/1
type: Range
persistent identity: range2354022
display id: range2354022
version: 1
direction: direction/forward
top level: BBa_K1031014
start: 904
end: 1050
orientation: inline

nahR
type: SequenceAnnotation
persistent identity: annotation2354021
display id: annotation2354021
version: 1
top level: BBa_K1031014
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1031014/annotation2354021/range2354021/1

https://synbiohub.org/public/igem/BBa_K1031014/annotation2354021/range2354021/1
type: Range
persistent identity: range2354021
display id: range2354021
version: 1
direction: direction/reverse
top level: BBa_K1031014
start: 1
end: 903
orientation: inline

cI
type: SequenceAnnotation
persistent identity: annotation2354024
display id: annotation2354024
version: 1
top level: BBa_K1031014
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1031014/annotation2354024/range2354024/1

https://synbiohub.org/public/igem/BBa_K1031014/annotation2354024/range2354024/1
type: Range
persistent identity: range2354024
display id: range2354024
version: 1
direction: direction/forward
top level: BBa_K1031014
start: 1170
end: 1886
orientation: inline

b0031
type: SequenceAnnotation
persistent identity: annotation2354023
display id: annotation2354023
version: 1
top level: BBa_K1031014
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1031014/annotation2354023/range2354023/1

https://synbiohub.org/public/igem/BBa_K1031014/annotation2354023/range2354023/1
type: Range
persistent identity: range2354023
display id: range2354023
version: 1
direction: direction/forward
top level: BBa_K1031014
start: 1150
end: 1163
orientation: inline

mnt (s)
type: ComponentDefinition
persistent identity: BBa C0072
display id: BBa_C0072
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: mnt repressor (strong) from Salmonella phage P22 (+LVA)
creator: crackdots
created: 2004-01-28T12:00:00Z
modified: 2015-08-31T04:07:23Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: -- No description --
mutable notes: wild type dimeric repressor from enterobacteriophage p22 with dissociation constant of 2.5*10^-12. sequence was translated with the E.Coli K12 codon usage table, and nucleotides 95-97 was changed from AAT to AAC to avoid the cut site GAATTC (94-99). Identification of Functionally Important Residues in the DNA Binding Region of the MNT Repressor Kendall, KL; Sauer, RT The Journal of Biological Chemistry, 264(23):13706-13710, 1989
mutable provenance: enterobacteriophage p22
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: T
sequence annotation: Help:Barcodes
sequence annotation: LVA
sequence annotation: mnt
sequence: https://synbiohub.org/public/igem/BBa_C0072_sequence/1

T
type: SequenceAnnotation
persistent identity: annotation308502
display id: annotation308502
version: 1
top level: mnt (s)
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_C0072/annotation308502/range308502/1

https://synbiohub.org/public/igem/BBa_C0072/annotation308502/range308502/1
type: Range
persistent identity: range308502
display id: range308502
version: 1
direction: direction/forward
top level: mnt (s)
start: 95
end: 97
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2214010
display id: annotation2214010
version: 1
top level: mnt (s)
role: SO:0000807
role: feature/barcode
location: https://synbiohub.org/public/igem/BBa_C0072/annotation2214010/range2214010/1

https://synbiohub.org/public/igem/BBa_C0072/annotation2214010/range2214010/1
type: Range
persistent identity: range2214010
display id: range2214010
version: 1
direction: direction/forward
top level: mnt (s)
start: 289
end: 313
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation308503
display id: annotation308503
version: 1
top level: mnt (s)
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_C0072/annotation308503/range308503/1

https://synbiohub.org/public/igem/BBa_C0072/annotation308503/range308503/1
type: Range
persistent identity: range308503
display id: range308503
version: 1
direction: direction/forward
top level: mnt (s)
start: 250
end: 282
orientation: inline

mnt
type: SequenceAnnotation
persistent identity: annotation308499
display id: annotation308499
version: 1
top level: mnt (s)
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_C0072/annotation308499/range308499/1

https://synbiohub.org/public/igem/BBa_C0072/annotation308499/range308499/1
type: Range
persistent identity: range308499
display id: range308499
version: 1
direction: direction/forward
top level: mnt (s)
start: 1
end: 249
orientation: inline

BBa_K374000
type: ComponentDefinition
persistent identity: BBa K374000
display id: BBa_K374000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: the Gifsy-1 phage lysogenic repressor gogR
creator: Anja Sander
created: 2010-09-01T11:00:00Z
modified: 2015-05-08T01:12:16Z
discontinued: true
dominant: false
experience: experience/None
group u list: _520_
m user id: 0
owner id: 6196
owning group id: 9
sample status: Discontinued
status: status/Deleted
bookmark: false
mutable description: GogR expression is controlled by the pRM promoter and for repression gogR can bind to three different operator sites OR1, OR2, OR3 on either prophage, which also overlap with the pR promoter, so pRM and pR share certain operator sites.
mutable notes: There were problems expressing gogR with a string RBS on a high copy number plasmid.
mutable provenance: Gifsy-1 is a lambdoid prophage present in Salmonella enterica
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316

BBa_K374002
type: ComponentDefinition
persistent identity: BBa K374002
display id: BBa_K374002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: the Gifsy-2 phage lysogenic repressor gtgR
creator: Anja Sander
created: 2010-09-01T11:00:00Z
modified: 2015-05-08T01:12:16Z
discontinued: true
dominant: false
experience: experience/None
group u list: _520_
m user id: 0
owner id: 6196
owning group id: 9
sample status: Discontinued
status: status/Deleted
bookmark: false
mutable description: GtgR is homologous to GogR and its expression is controlled by the pR promoter and for repression gtgR can bind to three different operator sites OR1, OR2, OR3 on either prophage, which also overlap with the pR promoter, so pRM and pR share certain operator sites.
mutable notes: No problems expressing gtgR with a strong RBS from a high copy number plasmid.
mutable provenance: Gifsy-1 is a lambdoid prophage present in Salmonella enterica. Gene sequence obtained from Sebastien Lemire, DTU.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316

BBa_K142003
type: ComponentDefinition
persistent identity: BBa K142003
display id: BBa_K142003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: lacI IS mutant (IPTG unresponsive) T276F
creator: Julius Rabl
created: 2008-10-16T11:00:00Z
modified: 2015-05-08T01:10:22Z
discontinued: false
dominant: false
group u list: _194_
m user id: 0
owner id: 3254
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Short description: The lacI IS mutant is almost identical to the lacI transcriptional regulator except for the difference that it is not able to bind IPTG or allolactose due to a mutation; it therefore can not be activated by induction with these substances. Since it recognizes the same motif in the lac promotor region, it strongly represses transcription of all genes regulated by promotors with lacI binding site even if IPTG or allolactose are present. It can be used to terminate the expression of proteins under lac control if IPTG can not be removed from the cell rapidly. Detailed description: Expression of the lac operon in E. coli is tightly controlled by lacI, a protein, which binds to a repressor binding site within the promotor and disables transcription by obscuring the promotor region. When bound to DNA, lacI is in the tetrameric form, which consists of two dimers interacting at the end distal from the DNA binding site. Upon binding of allolactose or IPTG, the tetramer breaks down into two dimers and the affinity for the repressor binding site is greatly reduced; the lacI IPTG complex will diffuse away from the repressor binding site, leaving the promotor accessible. As a result of decades of genetic and structural studies, the function of lacI is now understood on the molecular level (1, 2). Mutational experiments have identified residues, which abolish IPTG response upon mutation (3). Furthermore, the x-ray crystal structure of lacI with bound IPTG has allowed the identification of residues that interact with IPTG and which are promising targets for mutagenesis (1). We decided to mutate residues R197 and T276, which are located in the IPTG binding groove, contact IPTG and have been shown to produce the lacI IS mutation in previous genetic experiments. Since a quantitative study of the strength of inhibition by different lacI IS mutants has to our knowledge not been published so far, we decided to generate a set of eight mutated lacIs, in which we replaced…
mutable notes: During site-directed mutagenesis, the codons to be mutated were replaced with the most highly utilized codons in E. coli to prevent complications from the use of rare codons. The lacI IS sequences were analyzed for BioBrick restriction sites within the coding sequence to ensure their compatibility.
mutable provenance: The lacI IS mutants presented derive from the BioBrick holding lacI by itself (C0012). Site-directed mutagenesis was performed by PCR, subsequent DpnI digest and transformation. The following primers were used: R197F forward CGGCGCGTCTGTTTCTGGCTGGCTG R197A forward CGGCGCGTCTGGCGCTGGCTGGCTG R197F reverse CAGCCAGCCAGAAACAGACGCGCCG R197A reverse CAGCCAGCCAGCGCCAGACGCGCCG T276F forward GGATACGACGATTTTGAAGACAGCTC T276A forward GGATACGACGATGCGGAAGACAGCTC T276F reverse GAGCTGTCTTCAAAATCGTCGTATCC T276A reverse GAGCTGTCTTCCGCATCGTCGTATCC All lacI IS holding BioBricks were verified by sequencing.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: T276F
sequence annotation: lacI IS
sequence: https://synbiohub.org/public/igem/BBa_K142003_sequence/1

T276F
type: SequenceAnnotation
persistent identity: annotation1981804
display id: annotation1981804
version: 1
top level: BBa_K142003
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K142003/annotation1981804/range1981804/1

https://synbiohub.org/public/igem/BBa_K142003/annotation1981804/range1981804/1
type: Range
persistent identity: range1981804
display id: range1981804
version: 1
direction: direction/forward
top level: BBa_K142003
start: 835
end: 837
orientation: inline

lacI IS
type: SequenceAnnotation
persistent identity: annotation1981803
display id: annotation1981803
version: 1
top level: BBa_K142003
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K142003/annotation1981803/range1981803/1

https://synbiohub.org/public/igem/BBa_K142003/annotation1981803/range1981803/1
type: Range
persistent identity: range1981803
display id: range1981803
version: 1
direction: direction/forward
top level: BBa_K142003
start: 1
end: 1128
orientation: inline

BBa_K608351
type: ComponentDefinition
persistent identity: BBa K608351
display id: BBa_K608351
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: (correct) temperature sensitive promoter
creator: Theodhor Tiko
created: 2011-09-13T11:00:00Z
modified: 2015-05-08T01:12:52Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _780_
m user id: 0
owner id: 9113
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: correct temperatursesitive promotor
mutable notes: asd
mutable provenance: asd
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: BBa_J23114
sequence annotation: polya
sequence annotation: stop
sequence annotation: cIts
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: BBa_R0051
sequence annotation: BBa_K098997
sequence annotation: BBa_B0011
sequence annotation: stem_loop
sequence annotation: OR1
sequence annotation: OR2
sequence annotation: conserved
sequence annotation: -35
sequence annotation: -10
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K608351_sequence/1

BBa_J23114
type: SequenceAnnotation
persistent identity: annotation2131300
display id: annotation2131300
version: 1
top level: BBa_K608351
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K608351/annotation2131300/range2131300/1

https://synbiohub.org/public/igem/BBa_K608351/annotation2131300/range2131300/1
type: Range
persistent identity: range2131300
display id: range2131300
version: 1
direction: direction/reverse
top level: BBa_K608351
start: 1
end: 35
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation2131307
display id: annotation2131307
version: 1
top level: BBa_K608351
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_K608351/annotation2131307/range2131307/1

https://synbiohub.org/public/igem/BBa_K608351/annotation2131307/range2131307/1
type: Range
persistent identity: range2131307
display id: range2131307
version: 1
direction: direction/forward
top level: BBa_K608351
start: 824
end: 837
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2131308
display id: annotation2131308
version: 1
top level: BBa_K608351
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K608351/annotation2131308/range2131308/1

https://synbiohub.org/public/igem/BBa_K608351/annotation2131308/range2131308/1
type: Range
persistent identity: range2131308
display id: range2131308
version: 1
direction: direction/forward
top level: BBa_K608351
start: 830
end: 830
orientation: inline

cIts
type: SequenceAnnotation
persistent identity: annotation2131303
display id: annotation2131303
version: 1
top level: BBa_K608351
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K608351/annotation2131303/range2131303/1

https://synbiohub.org/public/igem/BBa_K608351/annotation2131303/range2131303/1
type: Range
persistent identity: range2131303
display id: range2131303
version: 1
direction: direction/forward
top level: BBa_K608351
start: 62
end: 775
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2131305
display id: annotation2131305
version: 1
top level: BBa_K608351
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K608351/annotation2131305/range2131305/1

https://synbiohub.org/public/igem/BBa_K608351/annotation2131305/range2131305/1
type: Range
persistent identity: range2131305
display id: range2131305
version: 1
direction: direction/forward
top level: BBa_K608351
start: 797
end: 837
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation2131306
display id: annotation2131306
version: 1
top level: BBa_K608351
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K608351/annotation2131306/range2131306/1

https://synbiohub.org/public/igem/BBa_K608351/annotation2131306/range2131306/1
type: Range
persistent identity: range2131306
display id: range2131306
version: 1
direction: direction/forward
top level: BBa_K608351
start: 804
end: 823
orientation: inline

BBa_R0051
type: SequenceAnnotation
persistent identity: annotation2131312
display id: annotation2131312
version: 1
top level: BBa_K608351
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K608351/annotation2131312/range2131312/1

https://synbiohub.org/public/igem/BBa_K608351/annotation2131312/range2131312/1
type: Range
persistent identity: range2131312
display id: range2131312
version: 1
direction: direction/forward
top level: BBa_K608351
start: 900
end: 948
orientation: inline

BBa_K098997
type: SequenceAnnotation
persistent identity: annotation2131304
display id: annotation2131304
version: 1
top level: BBa_K608351
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K608351/annotation2131304/range2131304/1

https://synbiohub.org/public/igem/BBa_K608351/annotation2131304/range2131304/1
type: Range
persistent identity: range2131304
display id: range2131304
version: 1
direction: direction/reverse
top level: BBa_K608351
start: 62
end: 775
orientation: inline

BBa_B0011
type: SequenceAnnotation
persistent identity: annotation2131309
display id: annotation2131309
version: 1
top level: BBa_K608351
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K608351/annotation2131309/range2131309/1

https://synbiohub.org/public/igem/BBa_K608351/annotation2131309/range2131309/1
type: Range
persistent identity: range2131309
display id: range2131309
version: 1
direction: direction/forward
top level: BBa_K608351
start: 846
end: 891
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation2131310
display id: annotation2131310
version: 1
top level: BBa_K608351
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K608351/annotation2131310/range2131310/1

https://synbiohub.org/public/igem/BBa_K608351/annotation2131310/range2131310/1
type: Range
persistent identity: range2131310
display id: range2131310
version: 1
direction: direction/forward
top level: BBa_K608351
start: 858
end: 880
orientation: inline

OR1
type: SequenceAnnotation
persistent identity: annotation2131314
display id: annotation2131314
version: 1
top level: BBa_K608351
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K608351/annotation2131314/range2131314/1

https://synbiohub.org/public/igem/BBa_K608351/annotation2131314/range2131314/1
type: Range
persistent identity: range2131314
display id: range2131314
version: 1
direction: direction/forward
top level: BBa_K608351
start: 924
end: 940
orientation: inline

OR2
type: SequenceAnnotation
persistent identity: annotation2131311
display id: annotation2131311
version: 1
top level: BBa_K608351
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K608351/annotation2131311/range2131311/1

https://synbiohub.org/public/igem/BBa_K608351/annotation2131311/range2131311/1
type: Range
persistent identity: range2131311
display id: range2131311
version: 1
direction: direction/forward
top level: BBa_K608351
start: 900
end: 916
orientation: inline

conserved
type: SequenceAnnotation
persistent identity: annotation2131425
display id: annotation2131425
version: 1
top level: BBa_K608351
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K608351/annotation2131425/range2131425/1

https://synbiohub.org/public/igem/BBa_K608351/annotation2131425/range2131425/1
type: Range
persistent identity: range2131425
display id: range2131425
version: 1
direction: direction/forward
top level: BBa_K608351
start: 776
end: 788
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2131313
display id: annotation2131313
version: 1
top level: BBa_K608351
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K608351/annotation2131313/range2131313/1

https://synbiohub.org/public/igem/BBa_K608351/annotation2131313/range2131313/1
type: Range
persistent identity: range2131313
display id: range2131313
version: 1
direction: direction/forward
top level: BBa_K608351
start: 914
end: 919
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2131315
display id: annotation2131315
version: 1
top level: BBa_K608351
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K608351/annotation2131315/range2131315/1

https://synbiohub.org/public/igem/BBa_K608351/annotation2131315/range2131315/1
type: Range
persistent identity: range2131315
display id: range2131315
version: 1
direction: direction/forward
top level: BBa_K608351
start: 937
end: 942
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2131302
display id: annotation2131302
version: 1
top level: BBa_K608351
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K608351/annotation2131302/range2131302/1

https://synbiohub.org/public/igem/BBa_K608351/annotation2131302/range2131302/1
type: Range
persistent identity: range2131302
display id: range2131302
version: 1
direction: direction/reverse
top level: BBa_K608351
start: 44
end: 55
orientation: inline

conserved
type: SequenceAnnotation
persistent identity: annotation2131301
display id: annotation2131301
version: 1
top level: BBa_K608351
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K608351/annotation2131301/range2131301/1

https://synbiohub.org/public/igem/BBa_K608351/annotation2131301/range2131301/1
type: Range
persistent identity: range2131301
display id: range2131301
version: 1
direction: direction/forward
top level: BBa_K608351
start: 48
end: 51
orientation: inline

LRLa
type: ComponentDefinition
persistent identity: BBa I732101
display id: BBa_I732101
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LRLa
creator: Zhan Jian
created: 2007-10-13T11:00:00Z
modified: 2015-08-31T04:07:57Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_I732101_sequence/1

BBa_K864202
type: ComponentDefinition
persistent identity: BBa K864202
display id: BBa_K864202
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: MarR(G95S) super-repressor
creator: Erik Lundin
created: 2012-09-24T11:00:00Z
modified: 2015-05-08T01:13:37Z
discontinued: false
dominant: false
group u list: _1124_
m user id: 0
owner id: 9827
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: marR(G95S) is a super-repressor
mutable notes: marR(G95S) carries a G>A mutation at position 284 which gives a amino acid change G95S. Has been mutated from BBa_K864200.
mutable provenance: BBa_K864200
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: cds
sequence annotation: G95S
sequence annotation: G>A
sequence: https://synbiohub.org/public/igem/BBa_K864202_sequence/1

cds
type: SequenceAnnotation
persistent identity: annotation2197126
display id: annotation2197126
version: 1
top level: BBa_K864202
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K864202/annotation2197126/range2197126/1

https://synbiohub.org/public/igem/BBa_K864202/annotation2197126/range2197126/1
type: Range
persistent identity: range2197126
display id: range2197126
version: 1
direction: direction/forward
top level: BBa_K864202
start: 1
end: 438
orientation: inline

G95S
type: SequenceAnnotation
persistent identity: annotation2197127
display id: annotation2197127
version: 1
top level: BBa_K864202
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K864202/annotation2197127/range2197127/1

https://synbiohub.org/public/igem/BBa_K864202/annotation2197127/range2197127/1
type: Range
persistent identity: range2197127
display id: range2197127
version: 1
direction: direction/forward
top level: BBa_K864202
start: 283
end: 285
orientation: inline

G>A
type: SequenceAnnotation
persistent identity: annotation2197128
display id: annotation2197128
version: 1
top level: BBa_K864202
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K864202/annotation2197128/range2197128/1

https://synbiohub.org/public/igem/BBa_K864202/annotation2197128/range2197128/1
type: Range
persistent identity: range2197128
display id: range2197128
version: 1
direction: direction/forward
top level: BBa_K864202
start: 283
end: 283
orientation: inline

cI HK022
type: ComponentDefinition
persistent identity: BBa C0050
display id: BBa_C0050
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: cI repressor from phage HK022 (+LVA?)
creator: Reshma Shetty
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:23Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Coding region for the HK022 bacteriophage cI protein. cI binds to the HK022 pR regulator (BBa_R0050). It represses transcription of the protein encoded by the sequence 3' to the pR region. This coding sequence does not contain a RBS.
mutable notes: References (unparsed) here: Carlson NG, Little JW. Highly cooperative DNA binding by the coliphage HK022 repressor. J Mol Biol. 1993 Apr 20;230(4):1108-30. PMID: 8487297. Mao C, Little JW. Mutations affecting cooperative DNA binding of phage HK022 CI repressor. J Mol Biol. 1998 May 29;279(1):31-48. PMID: 9636698. References (unparsed) here: Carlson NG, Little JW. Highly cooperative DNA binding by the coliphage HK022 repressor. J Mol Biol. 1993 Apr 20;230(4):1108-30. PMID: 8487297. Mao C, Little JW. Mutations affecting cooperative DNA binding of phage HK022 CI repressor. J Mol Biol. 1998 May 29;279(1):31-48. PMID: 9636698. Derived from <genbank>STHK022N</genbank>. Response from John Little (Arizona) regarding the start of HK022 cI From: <jlittle@email.arizona.edu> Date: Tue Jan 21, 2003 4:39:21 PM US/Eastern To: "Drew Endy" <endy@MIT.EDU> Subject: RE: hk022 cI start (naive question)? Hello Drew and Michael, I seriously doubt that the extra 27 aa are part of CI. It doesn't make sense in terms of the biology, for sure. In any case, the protein we characterized starts where Carlson and Little stated; as I recall, oR3 partially overlaps the start of cI. I have a vague memory of some possibly interesting biology, having to do with multicopy plasmids. I don't recall the findings themselves. Anyway, one possible explanation was that this extra N-terminal addition was made (e.g. from the pRE promoter, or from a message that arose from transcription around the entire plasmid), making a protein with altered functions. We never followed it up. Good luck John -- Original Message -- Date: Sun, 19 Jan 2003 15:26:26 -0500 Subject: hk022 cI start (naive question)? Cc: elowitm@rockefeller.edu To: jlittle@u.arizona.edu From: Drew Endy <endy@MIT.EDU> Hi John, I'm sitting here with Michael Elowitz and we're working through the sequence for HK022 cI. We noticed that the annotation from NC_002166 includes an "extra" 81 base pairs upstream of what we thought of as the actual start. It look…
mutable provenance: Bacteriophage HK022.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: 2
sequence annotation: BBa_C0050
sequence annotation: SsrA
sequence annotation: OR3 partial
sequence annotation: cI HK022
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C0050_sequence/1

2
type: SequenceAnnotation
persistent identity: annotation1734
display id: annotation1734
version: 1
top level: cI HK022
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_C0050/annotation1734/range1734/1

https://synbiohub.org/public/igem/BBa_C0050/annotation1734/range1734/1
type: Range
persistent identity: range1734
display id: range1734
version: 1
direction: direction/forward
top level: cI HK022
start: 739
end: 744
orientation: inline

BBa_C0050
type: SequenceAnnotation
persistent identity: annotation7033
display id: annotation7033
version: 1
top level: cI HK022
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_C0050/annotation7033/range7033/1

https://synbiohub.org/public/igem/BBa_C0050/annotation7033/range7033/1
type: Range
persistent identity: range7033
display id: range7033
version: 1
direction: direction/forward
top level: cI HK022
start: 1
end: 744
orientation: inline

SsrA
type: SequenceAnnotation
persistent identity: annotation1736
display id: annotation1736
version: 1
top level: cI HK022
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_C0050/annotation1736/range1736/1

https://synbiohub.org/public/igem/BBa_C0050/annotation1736/range1736/1
type: Range
persistent identity: range1736
display id: range1736
version: 1
direction: direction/forward
top level: cI HK022
start: 706
end: 738
orientation: inline

OR3 partial
type: SequenceAnnotation
persistent identity: annotation1737
display id: annotation1737
version: 1
top level: cI HK022
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_C0050/annotation1737/range1737/1

https://synbiohub.org/public/igem/BBa_C0050/annotation1737/range1737/1
type: Range
persistent identity: range1737
display id: range1737
version: 1
direction: direction/forward
top level: cI HK022
start: 1
end: 8
orientation: inline

cI HK022
type: SequenceAnnotation
persistent identity: annotation1735
display id: annotation1735
version: 1
top level: cI HK022
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_C0050/annotation1735/range1735/1

https://synbiohub.org/public/igem/BBa_C0050/annotation1735/range1735/1
type: Range
persistent identity: range1735
display id: range1735
version: 1
direction: direction/forward
top level: cI HK022
start: 1
end: 744
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213990
display id: annotation2213990
version: 1
top level: cI HK022
role: SO:0000807
role: feature/barcode
location: https://synbiohub.org/public/igem/BBa_C0050/annotation2213990/range2213990/1

https://synbiohub.org/public/igem/BBa_C0050/annotation2213990/range2213990/1
type: Range
persistent identity: range2213990
display id: range2213990
version: 1
direction: direction/forward
top level: cI HK022
start: 745
end: 769
orientation: inline

BBa_K1031743
type: ComponentDefinition
persistent identity: BBa K1031743
display id: BBa_K1031743
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: J23113-PaaX
creator: He Shuaixin
created: 2013-09-08T11:00:00Z
modified: 2015-05-08T01:08:47Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1338_
m user id: 0
owner id: 15843
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: 113-PaaX-TT
mutable notes: molecular cloning
mutable provenance: PCR genome E.coli
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: BBa_J23113
sequence annotation: BBa_B0034
sequence annotation: PaaX
sequence: https://synbiohub.org/public/igem/BBa_K1031743_sequence/1

BBa_J23113
type: SequenceAnnotation
persistent identity: annotation2357491
display id: annotation2357491
version: 1
top level: BBa_K1031743
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1031743/annotation2357491/range2357491/1

https://synbiohub.org/public/igem/BBa_K1031743/annotation2357491/range2357491/1
type: Range
persistent identity: range2357491
display id: range2357491
version: 1
direction: direction/forward
top level: BBa_K1031743
start: 1
end: 35
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2357492
display id: annotation2357492
version: 1
top level: BBa_K1031743
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1031743/annotation2357492/range2357492/1

https://synbiohub.org/public/igem/BBa_K1031743/annotation2357492/range2357492/1
type: Range
persistent identity: range2357492
display id: range2357492
version: 1
direction: direction/forward
top level: BBa_K1031743
start: 44
end: 55
orientation: inline

PaaX
type: SequenceAnnotation
persistent identity: annotation2357493
display id: annotation2357493
version: 1
top level: BBa_K1031743
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1031743/annotation2357493/range2357493/1

https://synbiohub.org/public/igem/BBa_K1031743/annotation2357493/range2357493/1
type: Range
persistent identity: range2357493
display id: range2357493
version: 1
direction: direction/forward
top level: BBa_K1031743
start: 62
end: 1012
orientation: inline

BBa_K1720003
type: ComponentDefinition
persistent identity: BBa K1720003
display id: BBa_K1720003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Human phosphodiesterase 5A gene silencing device NO.1
creator: Ying Guan, Yuanbin Cui
created: 2015-08-26T11:00:00Z
modified: 2015-09-18T07:29:39Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2140_
m user id: 25248
owner id: 25248
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This device is uesd for silencing the human phosphodiesterase 5A (PDE5A) gene.A U6 promoter driving a designed, synthetic shRNA-like miRNA followed by the terminator. PDE5A is a cGMP-binding, cGMP-specific phosphodiesterase, a member of the cyclic nucleotide phosphodiesterase family. This phosphodiesterase specifically hydrolyzes cGMP to 5'-GMP. It is involved in the regulation of intracellular concentrations of cyclic nucleotides and is important for smooth muscle relaxation in the cardiovascular system. We designed 3 silencing device and test their function at the same time.Here is the another two device :BBa_K1720004,BBaK172005 This device with a GFP reporter was then transfected into HEK293 cells by lentiviral vector.Once we silence the PDE5A gene the level of cGMP will be up regulated as a result. The positive control was HEK293 cells that treat with Sodium Nitroprusside ,a NO donator that activate sGC and up regulate the level of cGMP. A negative control was made by transfecting an empty vector that does not contain sGC subunit. We used Elisa to detect cGMP level. The results are as follow:
mutable notes: 1. Kukreja RC, Salloum FN, Das A: Cyclic Guanosine Monophosphate Signaling and Phosphodiesterase-5 Inhibitors in Cardioprotection. Journal Of the American College Of Cardiology 2012, 59(22):1921-1927. 2. Li L, Haider HK, Wang L, Lu G, Ashraf M: Adenoviral short hairpin RNA therapy targeting phosphodiesterase 5a relieves cardiac remodeling and dysfunction following myocardial infarction. American Journal Of Physiology-Heart And Circulatory Physiology 2012, 302(10):H2112-H2121. 3. Unwalla HJ, Li HT, Bahner I, Li MJ, Kohn D, Rossi JJ: Novel Pol II fusion promoter directs human immunodeficiency virus type 1-inducible coexpression of a short hairpin RNA and protein. Journal of virology 2006, 80(4):1863-1873.
mutable provenance: We designed the shRNA according to the homology segment sequence of PDE5A from NCBI
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
sequence: https://synbiohub.org/public/igem/BBa_K1720003_sequence/1

cI TP901
type: ComponentDefinition
persistent identity: BBa C0075
display id: BBa_C0075
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TP901 cI repressor from phage TP901-1 (+LVA)
creator: crackdots
created: 2004-01-27T12:00:00Z
modified: 2015-08-31T04:07:23Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: -- No description --
mutable notes: Dimeric repressor from bacteriophage TP901-1 with dissociation constant of 2*10^-9. Sequence was translated with the E.Coli K12 codon usage table, and nucleotides 84-86 was changed from TTC to TTT to avoid the cut site GAATTC (81-86). Bronsted, L., Hammer, K., Johansen, A.H., 2003. Identification of operator sites of the CI repressor of the phage TP901-1: evolutionary link to the other phages. Virology 311, 144-156. cI sequence slightly different from part C0051. NCBI locus: NC_001416
mutable provenance: TP901-1
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: LVA
sequence annotation: Help:Barcodes
sequence annotation: cI tp901
sequence: https://synbiohub.org/public/igem/BBa_C0075_sequence/1

LVA
type: SequenceAnnotation
persistent identity: annotation306574
display id: annotation306574
version: 1
top level: cI TP901
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_C0075/annotation306574/range306574/1

https://synbiohub.org/public/igem/BBa_C0075/annotation306574/range306574/1
type: Range
persistent identity: range306574
display id: range306574
version: 1
direction: direction/forward
top level: cI TP901
start: 541
end: 573
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2214007
display id: annotation2214007
version: 1
top level: cI TP901
role: SO:0000807
role: feature/barcode
location: https://synbiohub.org/public/igem/BBa_C0075/annotation2214007/range2214007/1

https://synbiohub.org/public/igem/BBa_C0075/annotation2214007/range2214007/1
type: Range
persistent identity: range2214007
display id: range2214007
version: 1
direction: direction/forward
top level: cI TP901
start: 580
end: 604
orientation: inline

cI tp901
type: SequenceAnnotation
persistent identity: annotation302645
display id: annotation302645
version: 1
top level: cI TP901
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_C0075/annotation302645/range302645/1

https://synbiohub.org/public/igem/BBa_C0075/annotation302645/range302645/1
type: Range
persistent identity: range302645
display id: range302645
version: 1
direction: direction/forward
top level: cI TP901
start: 1
end: 540
orientation: inline

lacI ts
type: ComponentDefinition
persistent identity: BBa J06500
display id: BBa_J06500
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LacI repressor (temperature-sensitive mut 241) (+LVA)
creator: ytwang
created: 2005-06-13T11:00:00Z
modified: 2015-08-31T04:08:18Z
discontinued: false
dominant: false
experience: experience/None
group u list: _20_
m user id: 0
owner id: 340
owning group id: 20
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Mutated from BBa_C0012 Repressor, LacI (LVA+) to be temperature sensitive. Mutation from Yabuta, 1995: Ala241->Thr (G(730)CG->ACG)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: LacI(ts)-LVA
sequence annotation: TS mutation (G->A)
sequence annotation: LVA
sequence annotation: barcode from LacI (BBa_C0012)
sequence: https://synbiohub.org/public/igem/BBa_J06500_sequence/1

LacI(ts)-LVA
type: SequenceAnnotation
persistent identity: annotation1518213
display id: annotation1518213
version: 1
top level: lacI ts
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J06500/annotation1518213/range1518213/1

https://synbiohub.org/public/igem/BBa_J06500/annotation1518213/range1518213/1
type: Range
persistent identity: range1518213
display id: range1518213
version: 1
direction: direction/forward
top level: lacI ts
start: 1
end: 1128
orientation: inline

TS mutation (G->A)
type: SequenceAnnotation
persistent identity: annotation1518230
display id: annotation1518230
version: 1
top level: lacI ts
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_J06500/annotation1518230/range1518230/1

https://synbiohub.org/public/igem/BBa_J06500/annotation1518230/range1518230/1
type: Range
persistent identity: range1518230
display id: range1518230
version: 1
direction: direction/forward
top level: lacI ts
start: 730
end: 730
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation1518220
display id: annotation1518220
version: 1
top level: lacI ts
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_J06500/annotation1518220/range1518220/1

https://synbiohub.org/public/igem/BBa_J06500/annotation1518220/range1518220/1
type: Range
persistent identity: range1518220
display id: range1518220
version: 1
direction: direction/forward
top level: lacI ts
start: 1090
end: 1128
orientation: inline

barcode from LacI (BBa_C0012)
type: SequenceAnnotation
persistent identity: annotation1518229
display id: annotation1518229
version: 1
top level: lacI ts
role: SO:0000807
role: feature/barcode
location: https://synbiohub.org/public/igem/BBa_J06500/annotation1518229/range1518229/1

https://synbiohub.org/public/igem/BBa_J06500/annotation1518229/range1518229/1
type: Range
persistent identity: range1518229
display id: range1518229
version: 1
direction: direction/forward
top level: lacI ts
start: 1129
end: 1153
orientation: inline

BBa_K142005
type: ComponentDefinition
persistent identity: BBa K142005
display id: BBa_K142005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: lacI IS mutant (IPTG unresponsive) R197A T276F
creator: Julius Rabl
created: 2008-10-24T11:00:00Z
modified: 2015-05-08T01:10:22Z
discontinued: false
dominant: false
experience: experience/None
group u list: _194_
m user id: 0
owner id: 3254
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: lacI IS mutant (IPTG unresponsive) Short description: The lacI IS mutant is almost identical to the lacI transcriptional regulator except for the difference that it is not able to bind IPTG or allolactose due to a mutation; it therefore can not be activated by induction with these substances. Since it recognizes the same motif in the lac promotor region, it strongly represses transcription of all genes regulated by promotors with lacI binding site even if IPTG or allolactose are present. It can be used to terminate the expression of proteins under lac control if IPTG can not be removed from the cell rapidly. Detailed description: Expression of the lac operon in E. coli is tightly controlled by lacI, a protein, which binds to a repressor binding site within the promotor and disables transcription by obscuring the promotor region. When bound to DNA, lacI is in the tetrameric form, which consists of two dimers interacting at the end distal from the DNA binding site. Upon binding of allolactose or IPTG, the tetramer breaks down into two dimers and the affinity for the repressor binding site is greatly reduced; the lacI IPTG complex will diffuse away from the repressor binding site, leaving the promotor accessible. As a result of decades of genetic and structural studies, the function of lacI is now understood on the molecular level (1, 2). Mutational experiments have identified residues, which abolish IPTG response upon mutation (3). Furthermore, the x-ray crystal structure of lacI with bound IPTG has allowed the identification of residues that interact with IPTG and which are promising targets for mutagenesis (1). We decided to mutate residues R197 and T276, which are located in the IPTG binding groove, contact IPTG and have been shown to produce the lacI IS mutation in previous genetic experiments. Since a quantitative study of the strength of inhibition by different lacI IS mutants has to our knowledge not been published so far, we decided to generate a set of eight …
mutable notes: During site-directed mutagenesis, the codons to be mutated were replaced with the most highly utilized codons in E. coli to prevent complications from the use of rare codons. The lacI IS sequences were analyzed for BioBrick restriction sites within the coding sequence to ensure their compatibility.
mutable provenance: The lacI IS mutants presented derive from the BioBrick holding lacI by itself (C0012). Site-directed mutagenesis was performed by PCR, subsequent DpnI digest and transformation. The following primers were used: R197F forward CGGCGCGTCTGTTTCTGGCTGGCTG R197A forward CGGCGCGTCTGGCGCTGGCTGGCTG R197F reverse CAGCCAGCCAGAAACAGACGCGCCG R197A reverse CAGCCAGCCAGCGCCAGACGCGCCG T276F forward GGATACGACGATTTTGAAGACAGCTC T276A forward GGATACGACGATGCGGAAGACAGCTC T276F reverse GAGCTGTCTTCAAAATCGTCGTATCC T276A reverse GAGCTGTCTTCCGCATCGTCGTATCC All lacI IS holding BioBricks were verified by sequencing.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: lacI IS
sequence: https://synbiohub.org/public/igem/BBa_K142005_sequence/1

lacI IS
type: SequenceAnnotation
persistent identity: annotation1987410
display id: annotation1987410
version: 1
top level: BBa_K142005
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K142005/annotation1987410/range1987410/1

https://synbiohub.org/public/igem/BBa_K142005/annotation1987410/range1987410/1
type: Range
persistent identity: range1987410
display id: range1987410
version: 1
direction: direction/forward
top level: BBa_K142005
start: 1
end: 1128
orientation: inline

BBa_K1520501
type: ComponentDefinition
persistent identity: BBa K1520501
display id: BBa_K1520501
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Rbs-luxR(scarless)
creator: yue wu
created: 2014-09-21T11:00:00Z
modified: 2015-05-08T01:10:49Z
discontinued: false
dominant: false
group u list: _1902_
m user id: 0
owner id: 21318
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Rbs-luxR(scarless)
mutable notes: None
mutable provenance: None
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
sequence annotation: luxR
sequence annotation: rbs
sequence: https://synbiohub.org/public/igem/BBa_K1520501_sequence/1

luxR
type: SequenceAnnotation
persistent identity: annotation2384862
display id: annotation2384862
version: 1
top level: BBa_K1520501
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1520501/annotation2384862/range2384862/1

https://synbiohub.org/public/igem/BBa_K1520501/annotation2384862/range2384862/1
type: Range
persistent identity: range2384862
display id: range2384862
version: 1
direction: direction/forward
top level: BBa_K1520501
start: 19
end: 799
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation2384861
display id: annotation2384861
version: 1
top level: BBa_K1520501
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1520501/annotation2384861/range2384861/1

https://synbiohub.org/public/igem/BBa_K1520501/annotation2384861/range2384861/1
type: Range
persistent identity: range2384861
display id: range2384861
version: 1
direction: direction/forward
top level: BBa_K1520501
start: 1
end: 18
orientation: inline

BBa_K1520503
type: ComponentDefinition
persistent identity: BBa K1520503
display id: BBa_K1520503
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Rbs-cI(scarless)
creator: yue wu
created: 2014-09-21T11:00:00Z
modified: 2015-05-08T01:10:49Z
discontinued: false
dominant: false
group u list: _1902_
m user id: 0
owner id: 21318
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Rbs-cI(scarless) It is an improvement of cI(BBa_C0051) by adding a rbs.
mutable notes: None
mutable provenance: It is an improvement of cI(BBa_C0051) by adding a rbs.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
sequence annotation: cI
sequence annotation: rbs
sequence: https://synbiohub.org/public/igem/BBa_K1520503_sequence/1

cI
type: SequenceAnnotation
persistent identity: annotation2384868
display id: annotation2384868
version: 1
top level: BBa_K1520503
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1520503/annotation2384868/range2384868/1

https://synbiohub.org/public/igem/BBa_K1520503/annotation2384868/range2384868/1
type: Range
persistent identity: range2384868
display id: range2384868
version: 1
direction: direction/forward
top level: BBa_K1520503
start: 19
end: 793
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation2384867
display id: annotation2384867
version: 1
top level: BBa_K1520503
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1520503/annotation2384867/range2384867/1

https://synbiohub.org/public/igem/BBa_K1520503/annotation2384867/range2384867/1
type: Range
persistent identity: range2384867
display id: range2384867
version: 1
direction: direction/forward
top level: BBa_K1520503
start: 1
end: 18
orientation: inline

BBa_K1031013
type: ComponentDefinition
persistent identity: BBa K1031013
display id: BBa_K1031013
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: NahR-Psal-RBS-RiboJ-CI
creator: He Shuaixin
created: 2013-09-08T11:00:00Z
modified: 2015-05-08T01:08:47Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1338_
m user id: 0
owner id: 15843
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: NahR Psel Ribo_J CI
mutable notes: Pseudomonas putida, phiR73, E.coli
mutable provenance: lab made
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: nahR
sequence annotation: cI
sequence annotation: promoter
sequence: https://synbiohub.org/public/igem/BBa_K1031013_sequence/1

nahR
type: SequenceAnnotation
persistent identity: annotation2353996
display id: annotation2353996
version: 1
top level: BBa_K1031013
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1031013/annotation2353996/range2353996/1

https://synbiohub.org/public/igem/BBa_K1031013/annotation2353996/range2353996/1
type: Range
persistent identity: range2353996
display id: range2353996
version: 1
direction: direction/reverse
top level: BBa_K1031013
start: 1
end: 903
orientation: inline

cI
type: SequenceAnnotation
persistent identity: annotation2353998
display id: annotation2353998
version: 1
top level: BBa_K1031013
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1031013/annotation2353998/range2353998/1

https://synbiohub.org/public/igem/BBa_K1031013/annotation2353998/range2353998/1
type: Range
persistent identity: range2353998
display id: range2353998
version: 1
direction: direction/forward
top level: BBa_K1031013
start: 1156
end: 1872
orientation: inline

promoter
type: SequenceAnnotation
persistent identity: annotation2353997
display id: annotation2353997
version: 1
top level: BBa_K1031013
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1031013/annotation2353997/range2353997/1

https://synbiohub.org/public/igem/BBa_K1031013/annotation2353997/range2353997/1
type: Range
persistent identity: range2353997
display id: range2353997
version: 1
direction: direction/forward
top level: BBa_K1031013
start: 904
end: 1050
orientation: inline

BBa_K1031016
type: ComponentDefinition
persistent identity: BBa K1031016
display id: BBa_K1031016
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: NahR-Psal-B0034-RiboJ-CI
creator: He Shuaixin
created: 2013-09-08T11:00:00Z
modified: 2015-05-08T01:08:47Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1338_
m user id: 0
owner id: 15843
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: NahR Psel Ribo_J CI B0034
mutable notes: Pseudomonas putida, phiR73 phage, E.coli
mutable provenance: lab made
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: cI
sequence annotation: B0034
sequence annotation: NahR
sequence annotation: promoter
sequence: https://synbiohub.org/public/igem/BBa_K1031016_sequence/1

cI
type: SequenceAnnotation
persistent identity: annotation2354032
display id: annotation2354032
version: 1
top level: BBa_K1031016
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1031016/annotation2354032/range2354032/1

https://synbiohub.org/public/igem/BBa_K1031016/annotation2354032/range2354032/1
type: Range
persistent identity: range2354032
display id: range2354032
version: 1
direction: direction/forward
top level: BBa_K1031016
start: 1169
end: 1885
orientation: inline

B0034
type: SequenceAnnotation
persistent identity: annotation2354031
display id: annotation2354031
version: 1
top level: BBa_K1031016
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1031016/annotation2354031/range2354031/1

https://synbiohub.org/public/igem/BBa_K1031016/annotation2354031/range2354031/1
type: Range
persistent identity: range2354031
display id: range2354031
version: 1
direction: direction/forward
top level: BBa_K1031016
start: 1150
end: 1165
orientation: inline

NahR
type: SequenceAnnotation
persistent identity: annotation2354029
display id: annotation2354029
version: 1
top level: BBa_K1031016
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1031016/annotation2354029/range2354029/1

https://synbiohub.org/public/igem/BBa_K1031016/annotation2354029/range2354029/1
type: Range
persistent identity: range2354029
display id: range2354029
version: 1
direction: direction/reverse
top level: BBa_K1031016
start: 1
end: 903
orientation: inline

promoter
type: SequenceAnnotation
persistent identity: annotation2354030
display id: annotation2354030
version: 1
top level: BBa_K1031016
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1031016/annotation2354030/range2354030/1

https://synbiohub.org/public/igem/BBa_K1031016/annotation2354030/range2354030/1
type: Range
persistent identity: range2354030
display id: range2354030
version: 1
direction: direction/forward
top level: BBa_K1031016
start: 904
end: 1050
orientation: inline

BBa_K1520018
type: ComponentDefinition
persistent identity: BBa K1520018
display id: BBa_K1520018
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Prlux-rbs-cI-Ter-PcI-rbs-lacI-Ter
creator: yue wu
created: 2014-09-21T11:00:00Z
modified: 2015-05-08T01:10:49Z
discontinued: false
dominant: false
group u list: _1902_
m user id: 0
owner id: 21318
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Plasmid2 in detector: Prlux-rbs-cI-Ter-PcI-rbs-lacI-Ter It is a low level detection plasmid. It produces rfp when AHL is enough.
mutable notes: None
mutable provenance: Prlux, cI, PcI, lacI,Ter
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1520006
component: BBa_K1520007
sequence annotation: BBa_K1520006
sequence annotation: BBa_K1520007
sequence: https://synbiohub.org/public/igem/BBa_K1520018_sequence/1

BBa_K1520006
type: Component
persistent identity: component2385063
display id: component2385063
version: 1
top level: BBa_K1520018
access: public
definition: BBa_K1520006

BBa_K1520007
type: Component
persistent identity: component2385073
display id: component2385073
version: 1
top level: BBa_K1520018
access: public
definition: BBa_K1520007

BBa_K1520006
type: SequenceAnnotation
persistent identity: annotation2385063
display id: annotation2385063
version: 1
top level: BBa_K1520018
location: https://synbiohub.org/public/igem/BBa_K1520018/annotation2385063/range2385063/1
component: BBa_K1520006

https://synbiohub.org/public/igem/BBa_K1520018/annotation2385063/range2385063/1
type: Range
persistent identity: range2385063
display id: range2385063
version: 1
direction: direction/reverse
top level: BBa_K1520018
start: 1
end: 993
orientation: inline

BBa_K1520007
type: SequenceAnnotation
persistent identity: annotation2385073
display id: annotation2385073
version: 1
top level: BBa_K1520018
location: https://synbiohub.org/public/igem/BBa_K1520018/annotation2385073/range2385073/1
component: BBa_K1520007

https://synbiohub.org/public/igem/BBa_K1520018/annotation2385073/range2385073/1
type: Range
persistent identity: range2385073
display id: range2385073
version: 1
direction: direction/reverse
top level: BBa_K1520018
start: 1002
end: 2366
orientation: inline

BBa_K1520502
type: ComponentDefinition
persistent identity: BBa K1520502
display id: BBa_K1520502
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Rbs-lacI(scarless)
creator: yue wu
created: 2014-09-21T11:00:00Z
modified: 2015-05-08T01:10:49Z
discontinued: false
dominant: false
group u list: _1902_
m user id: 0
owner id: 21318
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Rbs-lacI(scarless) It is an improvement of lacI by adding a rbs.
mutable notes: None
mutable provenance: It is an improvement of lacI by adding a rbs.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
sequence annotation: lacI
sequence annotation: rbs
sequence: https://synbiohub.org/public/igem/BBa_K1520502_sequence/1

lacI
type: SequenceAnnotation
persistent identity: annotation2384864
display id: annotation2384864
version: 1
top level: BBa_K1520502
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1520502/annotation2384864/range2384864/1

https://synbiohub.org/public/igem/BBa_K1520502/annotation2384864/range2384864/1
type: Range
persistent identity: range2384864
display id: range2384864
version: 1
direction: direction/forward
top level: BBa_K1520502
start: 19
end: 1171
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation2384863
display id: annotation2384863
version: 1
top level: BBa_K1520502
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1520502/annotation2384863/range2384863/1

https://synbiohub.org/public/igem/BBa_K1520502/annotation2384863/range2384863/1
type: Range
persistent identity: range2384863
display id: range2384863
version: 1
direction: direction/forward
top level: BBa_K1520502
start: 1
end: 18
orientation: inline

BBa_I715032
type: ComponentDefinition
persistent identity: BBa I715032
display id: BBa_I715032
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: lacIq reverse
creator: Jordan Baumgardner
created: 2007-07-17T11:00:00Z
modified: 2015-08-31T04:07:49Z
discontinued: false
dominant: false
experience: experience/None
group u list: _120_
m user id: 0
owner id: 1518
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This is a reverse version of BBa_C0012, the lacIq repressor cds.
mutable notes: Standard biobrick prefix and suffix were used. Since PCR was used, DNA sequence verification was needed - no mutations were found.
mutable provenance: PCR was used to amplify C0012 using primers that switched the biobrick prefix and suffix.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_I715032_sequence/1

BBa_K772104
type: ComponentDefinition
persistent identity: BBa K772104
display id: BBa_K772104
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pLac IPTG inducible generator
creator: Mustafa Elitok
created: 2012-10-04T11:00:00Z
modified: 2015-05-08T01:13:15Z
discontinued: false
dominant: false
group u list: _1024_
m user id: 0
owner id: 9573
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This part is an inverting regulator sensitive to LacI and CAP. It contains two protein binding sites. The first binds the CAP protein, which is generally present in E.coli and is asocciated with cell health and availability of glucose. The second binds LacI protein. This part also produces LacI protein as an oscillator which results that as LacI protein is synthesized, the promoter wil be inhibited until the presence of IPTG. The inhibition will decrease the production of LacI which leads to the synthesis of this inhibitor. >In the absence of LacI protein and CAP protein, this part promotes transcription. >In the presence of LacI protein and CAP protein, this part inhibits transcription. >LacI can be inhibited by IPTG. >LacI is coded by BBa_C0010
mutable notes: Note that this part is not working unless IPTG is present in the media.
mutable provenance: Registry
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
component: BBa_J04500
component: BBa_C0012
sequence annotation: BBa_C0012
sequence annotation: BBa_J04500
sequence: https://synbiohub.org/public/igem/BBa_K772104_sequence/1

BBa_J04500
type: Component
persistent identity: component2248259
display id: component2248259
version: 1
top level: BBa_K772104
access: public
definition: https://synbiohub.org/public/igem/BBa_J04500/1

BBa_C0012
type: Component
persistent identity: component2248261
display id: component2248261
version: 1
top level: BBa_K772104
access: public
definition: lacI

BBa_C0012
type: SequenceAnnotation
persistent identity: annotation2248261
display id: annotation2248261
version: 1
top level: BBa_K772104
location: https://synbiohub.org/public/igem/BBa_K772104/annotation2248261/range2248261/1
component: BBa_C0012

https://synbiohub.org/public/igem/BBa_K772104/annotation2248261/range2248261/1
type: Range
persistent identity: range2248261
display id: range2248261
version: 1
direction: direction/forward
top level: BBa_K772104
start: 227
end: 1354
orientation: inline

BBa_J04500
type: SequenceAnnotation
persistent identity: annotation2248259
display id: annotation2248259
version: 1
top level: BBa_K772104
location: https://synbiohub.org/public/igem/BBa_K772104/annotation2248259/range2248259/1
component: BBa_J04500

https://synbiohub.org/public/igem/BBa_K772104/annotation2248259/range2248259/1
type: Range
persistent identity: range2248259
display id: range2248259
version: 1
direction: direction/reverse
top level: BBa_K772104
start: 1
end: 220
orientation: inline

BBa_K1520006
type: ComponentDefinition
persistent identity: BBa K1520006
display id: BBa_K1520006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Prlux-rbs-cI-Ter
creator: yue wu
created: 2014-09-21T11:00:00Z
modified: 2015-05-08T01:10:48Z
discontinued: false
dominant: false
group u list: _1902_
m user id: 0
owner id: 21318
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Prlux-rbs-cI-Ter. It produces cI when there is enough luxR and AHL.
mutable notes: None
mutable provenance: Prlux, cI, Ter
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1520029
component: BBa_R0062
component: BBa_K1520503
sequence annotation: BBa_R0062
sequence annotation: BBa_K1520029
sequence annotation: BBa_K1520503
sequence: https://synbiohub.org/public/igem/BBa_K1520006_sequence/1

BBa_K1520029
type: Component
persistent identity: component2384919
display id: component2384919
version: 1
top level: BBa_K1520006
access: public
definition: https://synbiohub.org/public/igem/BBa_K1520029/1

BBa_R0062
type: Component
persistent identity: component2384911
display id: component2384911
version: 1
top level: BBa_K1520006
access: public
definition: https://synbiohub.org/public/igem/BBa_R0062/1

BBa_K1520503
type: Component
persistent identity: component2384918
display id: component2384918
version: 1
top level: BBa_K1520006
access: public
definition: BBa_K1520503

BBa_R0062
type: SequenceAnnotation
persistent identity: annotation2384911
display id: annotation2384911
version: 1
top level: BBa_K1520006
location: https://synbiohub.org/public/igem/BBa_K1520006/annotation2384911/range2384911/1
component: BBa_R0062

https://synbiohub.org/public/igem/BBa_K1520006/annotation2384911/range2384911/1
type: Range
persistent identity: range2384911
display id: range2384911
version: 1
direction: direction/forward
top level: BBa_K1520006
start: 1
end: 55
orientation: inline

BBa_K1520029
type: SequenceAnnotation
persistent identity: annotation2384919
display id: annotation2384919
version: 1
top level: BBa_K1520006
location: https://synbiohub.org/public/igem/BBa_K1520006/annotation2384919/range2384919/1
component: BBa_K1520029

https://synbiohub.org/public/igem/BBa_K1520006/annotation2384919/range2384919/1
type: Range
persistent identity: range2384919
display id: range2384919
version: 1
direction: direction/reverse
top level: BBa_K1520006
start: 865
end: 993
orientation: inline

BBa_K1520503
type: SequenceAnnotation
persistent identity: annotation2384918
display id: annotation2384918
version: 1
top level: BBa_K1520006
location: https://synbiohub.org/public/igem/BBa_K1520006/annotation2384918/range2384918/1
component: BBa_K1520503

https://synbiohub.org/public/igem/BBa_K1520006/annotation2384918/range2384918/1
type: Range
persistent identity: range2384918
display id: range2384918
version: 1
direction: direction/reverse
top level: BBa_K1520006
start: 64
end: 856
orientation: inline

BBa_K544012
type: ComponentDefinition
persistent identity: BBa K544012
display id: BBa_K544012
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Tetracycline Repressor with Consitutive Promoter BBa_J23106
creator: Leung Man Hong
created: 2011-09-29T11:00:00Z
modified: 2015-05-08T01:12:39Z
discontinued: false
dominant: false
group u list: _711_
m user id: 0
owner id: 6780
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: A transcriptional regulator TetR (BBa_C0040) is driven by constitutive promoter family members (BBa-J23103, BBa-J23109, BBa-J23116, and BBa-J23106), which can be used to tune the expression level of constitutively expressed TetR.
mutable notes: The SsrA site at the end of BBa_C0040 is not present in our design.
mutable provenance: The source of this part is based on the parts in the registry distribution
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: BBa_B0034
sequence annotation: BBa_J23106
sequence annotation: tet-R
sequence: https://synbiohub.org/public/igem/BBa_K544012_sequence/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2148190
display id: annotation2148190
version: 1
top level: BBa_K544012
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K544012/annotation2148190/range2148190/1

https://synbiohub.org/public/igem/BBa_K544012/annotation2148190/range2148190/1
type: Range
persistent identity: range2148190
display id: range2148190
version: 1
direction: direction/forward
top level: BBa_K544012
start: 44
end: 55
orientation: inline

BBa_J23106
type: SequenceAnnotation
persistent identity: annotation2148189
display id: annotation2148189
version: 1
top level: BBa_K544012
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K544012/annotation2148189/range2148189/1

https://synbiohub.org/public/igem/BBa_K544012/annotation2148189/range2148189/1
type: Range
persistent identity: range2148189
display id: range2148189
version: 1
direction: direction/forward
top level: BBa_K544012
start: 1
end: 35
orientation: inline

tet-R
type: SequenceAnnotation
persistent identity: annotation2148191
display id: annotation2148191
version: 1
top level: BBa_K544012
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K544012/annotation2148191/range2148191/1

https://synbiohub.org/public/igem/BBa_K544012/annotation2148191/range2148191/1
type: Range
persistent identity: range2148191
display id: range2148191
version: 1
direction: direction/forward
top level: BBa_K544012
start: 62
end: 682
orientation: inline

BBa_K106669
type: ComponentDefinition
persistent identity: BBa K106669
display id: BBa_K106669
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Tet Repressor, AarI BD part
creator: Andrej Ondracka
created: 2008-10-24T11:00:00Z
modified: 2015-05-08T01:08:59Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _226_
m user id: 0
owner id: 3416
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: Tet repressor from E.coli
mutable notes: .
mutable provenance: .
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K106669_sequence/1

ARL2A0103
type: ComponentDefinition
persistent identity: BBa I732107
display id: BBa_I732107
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: ARL2A0103
creator: Zhan Jian
created: 2007-10-13T11:00:00Z
modified: 2015-08-31T04:07:57Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_I732107_sequence/1

c2 P22
type: ComponentDefinition
persistent identity: BBa C0053
display id: BBa_C0053
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: c2 repressor from Salmonella phage P22 (+LVA)
creator: Maia Mahoney
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:23Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: The P22 c2 repressor protein coding sequence is a 720 base-pair sequence with the standard RBS-compatible BioBrick prefix and the standard BioBrick suffix sections on its ends. It binds to the P22 c2 regulatory sequence, BBa_R0053. The sequence contains a LVA tag for faster degredation.
mutable notes: References (unparsed) here: Vander Byl,C. and Kropinski,A.M. Sequence of the genome of Salmonella bacteriophage P22 J. Bacteriol. 182 (22), 6472-6481 (2000) References (unparsed) here: Vander Byl,C. and Kropinski,A.M. Sequence of the genome of Salmonella bacteriophage P22 J. Bacteriol. 182 (22), 6472-6481 (2000)
mutable provenance: Bacteriophage P22
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: stop
sequence annotation: LVA
sequence annotation: BBa_C0053
sequence annotation: cII p22
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C0053_sequence/1

stop
type: SequenceAnnotation
persistent identity: annotation1751
display id: annotation1751
version: 1
top level: c2 P22
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_C0053/annotation1751/range1751/1

https://synbiohub.org/public/igem/BBa_C0053/annotation1751/range1751/1
type: Range
persistent identity: range1751
display id: range1751
version: 1
direction: direction/forward
top level: c2 P22
start: 682
end: 687
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation1750
display id: annotation1750
version: 1
top level: c2 P22
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_C0053/annotation1750/range1750/1

https://synbiohub.org/public/igem/BBa_C0053/annotation1750/range1750/1
type: Range
persistent identity: range1750
display id: range1750
version: 1
direction: direction/forward
top level: c2 P22
start: 649
end: 681
orientation: inline

BBa_C0053
type: SequenceAnnotation
persistent identity: annotation7036
display id: annotation7036
version: 1
top level: c2 P22
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_C0053/annotation7036/range7036/1

https://synbiohub.org/public/igem/BBa_C0053/annotation7036/range7036/1
type: Range
persistent identity: range7036
display id: range7036
version: 1
direction: direction/forward
top level: c2 P22
start: 1
end: 687
orientation: inline

cII p22
type: SequenceAnnotation
persistent identity: annotation1747
display id: annotation1747
version: 1
top level: c2 P22
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_C0053/annotation1747/range1747/1

https://synbiohub.org/public/igem/BBa_C0053/annotation1747/range1747/1
type: Range
persistent identity: range1747
display id: range1747
version: 1
direction: direction/forward
top level: c2 P22
start: 1
end: 648
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213993
display id: annotation2213993
version: 1
top level: c2 P22
role: SO:0000807
role: feature/barcode
location: https://synbiohub.org/public/igem/BBa_C0053/annotation2213993/range2213993/1

https://synbiohub.org/public/igem/BBa_C0053/annotation2213993/range2213993/1
type: Range
persistent identity: range2213993
display id: range2213993
version: 1
direction: direction/forward
top level: c2 P22
start: 688
end: 712
orientation: inline

BBa_K1520013
type: ComponentDefinition
persistent identity: BBa K1520013
display id: BBa_K1520013
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Prlux-rbs-rfp-rbs-luxI-rbs-luxR-Ter
creator: yue wu
created: 2014-09-21T11:00:00Z
modified: 2015-05-08T01:10:49Z
discontinued: false
dominant: false
group u list: _1902_
m user id: 0
owner id: 21318
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Prlux-rbs-rfp-rbs-luxI-rbs-luxR-Ter. It produces rfp, luxI and luxR when there is enough luxR and AHL.
mutable notes: None
mutable provenance: Prlux, rfp, luxI, luxR, Ter
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1520501
component: BBa_K1520029
component: BBa_R0062
component: BBa_K1520500
component: BBa_K1520504
sequence annotation: BBa_K1520500
sequence annotation: BBa_K1520504
sequence annotation: BBa_K1520501
sequence annotation: BBa_K1520029
sequence annotation: BBa_R0062
sequence: https://synbiohub.org/public/igem/BBa_K1520013_sequence/1

BBa_K1520501
type: Component
persistent identity: component2384990
display id: component2384990
version: 1
top level: BBa_K1520013
access: public
definition: BBa_K1520501

BBa_K1520029
type: Component
persistent identity: component2384991
display id: component2384991
version: 1
top level: BBa_K1520013
access: public
definition: https://synbiohub.org/public/igem/BBa_K1520029/1

BBa_R0062
type: Component
persistent identity: component2384979
display id: component2384979
version: 1
top level: BBa_K1520013
access: public
definition: https://synbiohub.org/public/igem/BBa_R0062/1

BBa_K1520500
type: Component
persistent identity: component2384984
display id: component2384984
version: 1
top level: BBa_K1520013
access: public
definition: https://synbiohub.org/public/igem/BBa_K1520500/1

BBa_K1520504
type: Component
persistent identity: component2384987
display id: component2384987
version: 1
top level: BBa_K1520013
access: public
definition: https://synbiohub.org/public/igem/BBa_K1520504/1

BBa_K1520500
type: SequenceAnnotation
persistent identity: annotation2384984
display id: annotation2384984
version: 1
top level: BBa_K1520013
location: https://synbiohub.org/public/igem/BBa_K1520013/annotation2384984/range2384984/1
component: BBa_K1520500

https://synbiohub.org/public/igem/BBa_K1520013/annotation2384984/range2384984/1
type: Range
persistent identity: range2384984
display id: range2384984
version: 1
direction: direction/reverse
top level: BBa_K1520013
start: 64
end: 795
orientation: inline

BBa_K1520504
type: SequenceAnnotation
persistent identity: annotation2384987
display id: annotation2384987
version: 1
top level: BBa_K1520013
location: https://synbiohub.org/public/igem/BBa_K1520013/annotation2384987/range2384987/1
component: BBa_K1520504

https://synbiohub.org/public/igem/BBa_K1520013/annotation2384987/range2384987/1
type: Range
persistent identity: range2384987
display id: range2384987
version: 1
direction: direction/reverse
top level: BBa_K1520013
start: 804
end: 1464
orientation: inline

BBa_K1520501
type: SequenceAnnotation
persistent identity: annotation2384990
display id: annotation2384990
version: 1
top level: BBa_K1520013
location: https://synbiohub.org/public/igem/BBa_K1520013/annotation2384990/range2384990/1
component: BBa_K1520501

https://synbiohub.org/public/igem/BBa_K1520013/annotation2384990/range2384990/1
type: Range
persistent identity: range2384990
display id: range2384990
version: 1
direction: direction/reverse
top level: BBa_K1520013
start: 1473
end: 2271
orientation: inline

BBa_K1520029
type: SequenceAnnotation
persistent identity: annotation2384991
display id: annotation2384991
version: 1
top level: BBa_K1520013
location: https://synbiohub.org/public/igem/BBa_K1520013/annotation2384991/range2384991/1
component: BBa_K1520029

https://synbiohub.org/public/igem/BBa_K1520013/annotation2384991/range2384991/1
type: Range
persistent identity: range2384991
display id: range2384991
version: 1
direction: direction/reverse
top level: BBa_K1520013
start: 2280
end: 2408
orientation: inline

BBa_R0062
type: SequenceAnnotation
persistent identity: annotation2384979
display id: annotation2384979
version: 1
top level: BBa_K1520013
location: https://synbiohub.org/public/igem/BBa_K1520013/annotation2384979/range2384979/1
component: BBa_R0062

https://synbiohub.org/public/igem/BBa_K1520013/annotation2384979/range2384979/1
type: Range
persistent identity: range2384979
display id: range2384979
version: 1
direction: direction/forward
top level: BBa_K1520013
start: 1
end: 55
orientation: inline

BBa_K1720005
type: ComponentDefinition
persistent identity: BBa K1720005
display id: BBa_K1720005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Human phosphodiesterase 5A gene silencing device NO.3
creator: Ying Guan , Yuanbin Cui
created: 2015-08-26T11:00:00Z
modified: 2015-09-18T07:28:43Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2140_
m user id: 25248
owner id: 25248
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This device is uesd for silencing the human phosphodiesterase 5A (PDE5A) gene.A U6 promoter driving a designed, synthetic shRNA-like miRNA followed by the terminator. PDE5A is a cGMP-binding, cGMP-specific phosphodiesterase, a member of the cyclic nucleotide phosphodiesterase family. This phosphodiesterase specifically hydrolyzes cGMP to 5'-GMP. It is involved in the regulation of intracellular concentrations of cyclic nucleotides and is important for smooth muscle relaxation in the cardiovascular system. We designed 3 silencing device and test their function at the same time.Here is the another two device :BBa_K1720004,BBaK172005 This device with a GFP reporter was then transfected into HEK293 cells by lentiviral vector.Once we silence the PDE5A gene the level of cGMP will be up regulated as a result. The positive control was HEK293 cells that treat with Sodium Nitroprusside ,a NO donator that activate sGC and up regulate the level of cGMP. A negative control was made by transfecting an empty vector that does not contain sGC subunit. We used Elisa to detect cGMP level. The results are as follow:
mutable notes: 1. Kukreja RC, Salloum FN, Das A: Cyclic Guanosine Monophosphate Signaling and Phosphodiesterase-5 Inhibitors in Cardioprotection. Journal Of the American College Of Cardiology 2012, 59(22):1921-1927. 2. Li L, Haider HK, Wang L, Lu G, Ashraf M: Adenoviral short hairpin RNA therapy targeting phosphodiesterase 5a relieves cardiac remodeling and dysfunction following myocardial infarction. American Journal Of Physiology-Heart And Circulatory Physiology 2012, 302(10):H2112-H2121. 3. Unwalla HJ, Li HT, Bahner I, Li MJ, Kohn D, Rossi JJ: Novel Pol II fusion promoter directs human immunodeficiency virus type 1-inducible coexpression of a short hairpin RNA and protein. Journal of virology 2006, 80(4):1863-1873.
mutable provenance: We designed the shRNA according to the homology segment sequence of PDE5A from NCBI
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
sequence: https://synbiohub.org/public/igem/BBa_K1720005_sequence/1

BBa_K1223001
type: ComponentDefinition
persistent identity: BBa K1223001
display id: BBa_K1223001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: P.A.S.E. 1 cassette
creator: Orr Schlesinger
created: 2013-09-06T11:00:00Z
modified: 2015-05-08T01:09:43Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1537_
m user id: 0
owner id: 18143
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: A DNA cassette that undergo homologous recombination into E.coli genome that contains homologous regions at each end, Kanamicin resistance gene (KanR) and death operon containing Lambda holin and lysozime genes and controlled by a Lambda cI regulated promoter
mutable notes: /
mutable provenance: part is synthetic. holin and lysozime originates from lambda bacteriophage. the homology regions were taken from E.coli BL21 genome
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: Lambda holin CDS
sequence annotation: KanR CDS
sequence annotation: P.A.S.E 1 death operaton
sequence annotation: upstream homology region
sequence annotation: Lambda lyzosime
sequence annotation: downstream homology region
sequence annotation: cI regulated promoter
sequence: https://synbiohub.org/public/igem/BBa_K1223001_sequence/1

Lambda holin CDS
type: SequenceAnnotation
persistent identity: annotation2336783
display id: annotation2336783
version: 1
top level: BBa_K1223001
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1223001/annotation2336783/range2336783/1

https://synbiohub.org/public/igem/BBa_K1223001/annotation2336783/range2336783/1
type: Range
persistent identity: range2336783
display id: range2336783
version: 1
direction: direction/forward
top level: BBa_K1223001
start: 1631
end: 1948
orientation: inline

KanR CDS
type: SequenceAnnotation
persistent identity: annotation2336779
display id: annotation2336779
version: 1
top level: BBa_K1223001
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1223001/annotation2336779/range2336779/1

https://synbiohub.org/public/igem/BBa_K1223001/annotation2336779/range2336779/1
type: Range
persistent identity: range2336779
display id: range2336779
version: 1
direction: direction/forward
top level: BBa_K1223001
start: 234
end: 1049
orientation: inline

P.A.S.E 1 death operaton
type: SequenceAnnotation
persistent identity: annotation2336781
display id: annotation2336781
version: 1
top level: BBa_K1223001
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K1223001/annotation2336781/range2336781/1

https://synbiohub.org/public/igem/BBa_K1223001/annotation2336781/range2336781/1
type: Range
persistent identity: range2336781
display id: range2336781
version: 1
direction: direction/forward
top level: BBa_K1223001
start: 1059
end: 2088
orientation: inline

upstream homology region
type: SequenceAnnotation
persistent identity: annotation2336778
display id: annotation2336778
version: 1
top level: BBa_K1223001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1223001/annotation2336778/range2336778/1

https://synbiohub.org/public/igem/BBa_K1223001/annotation2336778/range2336778/1
type: Range
persistent identity: range2336778
display id: range2336778
version: 1
direction: direction/forward
top level: BBa_K1223001
start: 19
end: 73
orientation: inline

Lambda lyzosime
type: SequenceAnnotation
persistent identity: annotation2336782
display id: annotation2336782
version: 1
top level: BBa_K1223001
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1223001/annotation2336782/range2336782/1

https://synbiohub.org/public/igem/BBa_K1223001/annotation2336782/range2336782/1
type: Range
persistent identity: range2336782
display id: range2336782
version: 1
direction: direction/forward
top level: BBa_K1223001
start: 1134
end: 1610
orientation: inline

downstream homology region
type: SequenceAnnotation
persistent identity: annotation2336784
display id: annotation2336784
version: 1
top level: BBa_K1223001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1223001/annotation2336784/range2336784/1

https://synbiohub.org/public/igem/BBa_K1223001/annotation2336784/range2336784/1
type: Range
persistent identity: range2336784
display id: range2336784
version: 1
direction: direction/forward
top level: BBa_K1223001
start: 2094
end: 2148
orientation: inline

cI regulated promoter
type: SequenceAnnotation
persistent identity: annotation2336780
display id: annotation2336780
version: 1
top level: BBa_K1223001
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1223001/annotation2336780/range2336780/1

https://synbiohub.org/public/igem/BBa_K1223001/annotation2336780/range2336780/1
type: Range
persistent identity: range2336780
display id: range2336780
version: 1
direction: direction/forward
top level: BBa_K1223001
start: 1059
end: 1107
orientation: inline

zif23
type: ComponentDefinition
persistent identity: BBa C2001
display id: BBa_C2001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Zif23-GCN4 engineered repressor (+LVA, C2000 codon-optimized for E.coli)
creator: Drew Endy
created: 2004-01-29T12:00:00Z
modified: 2015-08-31T04:07:25Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Variant of Zif23-GCN4 (see BBa_C2000). CDS has been optimized (via VNTI) for expression in enteric bacteria. ssrA tag (LVA) added.
mutable notes: This protein should form a dimer and bind a specific operator site, thus repressing transcription.
mutable provenance: Protein Engineering Gods from the Pabo Lab
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: linker
sequence annotation: Recognition site
sequence annotation: Help:Barcodes
sequence annotation: 2
sequence annotation: Finger 2
sequence annotation: Finger 3
sequence annotation: LVA
sequence annotation: GCN4: leucine zipper
sequence: https://synbiohub.org/public/igem/BBa_C2001_sequence/1

linker
type: SequenceAnnotation
persistent identity: annotation318104
display id: annotation318104
version: 1
top level: zif23
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_C2001/annotation318104/range318104/1

https://synbiohub.org/public/igem/BBa_C2001/annotation318104/range318104/1
type: Range
persistent identity: range318104
display id: range318104
version: 1
direction: direction/forward
top level: zif23
start: 151
end: 165
orientation: inline

Recognition site
type: SequenceAnnotation
persistent identity: annotation318109
display id: annotation318109
version: 1
top level: zif23
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_C2001/annotation318109/range318109/1

https://synbiohub.org/public/igem/BBa_C2001/annotation318109/range318109/1
type: Range
persistent identity: range318109
display id: range318109
version: 1
direction: direction/forward
top level: zif23
start: 127
end: 147
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2214011
display id: annotation2214011
version: 1
top level: zif23
role: SO:0000807
role: feature/barcode
location: https://synbiohub.org/public/igem/BBa_C2001/annotation2214011/range2214011/1

https://synbiohub.org/public/igem/BBa_C2001/annotation2214011/range2214011/1
type: Range
persistent identity: range2214011
display id: range2214011
version: 1
direction: direction/forward
top level: zif23
start: 301
end: 325
orientation: inline

2
type: SequenceAnnotation
persistent identity: annotation318107
display id: annotation318107
version: 1
top level: zif23
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_C2001/annotation318107/range318107/1

https://synbiohub.org/public/igem/BBa_C2001/annotation318107/range318107/1
type: Range
persistent identity: range318107
display id: range318107
version: 1
direction: direction/forward
top level: zif23
start: 295
end: 300
orientation: inline

Finger 2
type: SequenceAnnotation
persistent identity: annotation318101
display id: annotation318101
version: 1
top level: zif23
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_C2001/annotation318101/range318101/1

https://synbiohub.org/public/igem/BBa_C2001/annotation318101/range318101/1
type: Range
persistent identity: range318101
display id: range318101
version: 1
direction: direction/forward
top level: zif23
start: 4
end: 90
orientation: inline

Finger 3
type: SequenceAnnotation
persistent identity: annotation318103
display id: annotation318103
version: 1
top level: zif23
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_C2001/annotation318103/range318103/1

https://synbiohub.org/public/igem/BBa_C2001/annotation318103/range318103/1
type: Range
persistent identity: range318103
display id: range318103
version: 1
direction: direction/forward
top level: zif23
start: 91
end: 150
orientation: inline

Recognition site
type: SequenceAnnotation
persistent identity: annotation318108
display id: annotation318108
version: 1
top level: zif23
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_C2001/annotation318108/range318108/1

https://synbiohub.org/public/igem/BBa_C2001/annotation318108/range318108/1
type: Range
persistent identity: range318108
display id: range318108
version: 1
direction: direction/forward
top level: zif23
start: 43
end: 63
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation318106
display id: annotation318106
version: 1
top level: zif23
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_C2001/annotation318106/range318106/1

https://synbiohub.org/public/igem/BBa_C2001/annotation318106/range318106/1
type: Range
persistent identity: range318106
display id: range318106
version: 1
direction: direction/forward
top level: zif23
start: 262
end: 294
orientation: inline

GCN4: leucine zipper
type: SequenceAnnotation
persistent identity: annotation318105
display id: annotation318105
version: 1
top level: zif23
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_C2001/annotation318105/range318105/1

https://synbiohub.org/public/igem/BBa_C2001/annotation318105/range318105/1
type: Range
persistent identity: range318105
display id: range318105
version: 1
direction: direction/forward
top level: zif23
start: 166
end: 261
orientation: inline

BBa_K082004
type: ComponentDefinition
persistent identity: BBa K082004
display id: BBa_K082004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LacI- wild type
creator: Yanting Xue , He Shen
created: 2008-10-27T12:00:00Z
modified: 2015-05-08T01:08:35Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _228_
m user id: 0
owner id: 1560
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: The
mutable notes: The
mutable provenance: The
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: BBa_K082004
sequence: https://synbiohub.org/public/igem/BBa_K082004_sequence/1

BBa_K082004
type: SequenceAnnotation
persistent identity: annotation1992777
display id: annotation1992777
version: 1
top level: BBa_K082004
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K082004/annotation1992777/range1992777/1

https://synbiohub.org/public/igem/BBa_K082004/annotation1992777/range1992777/1
type: Range
persistent identity: range1992777
display id: range1992777
version: 1
direction: direction/reverse
top level: BBa_K082004
start: 1
end: 1083
orientation: inline

BBa_K1031015
type: ComponentDefinition
persistent identity: BBa K1031015
display id: BBa_K1031015
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: NahR-Psal-B0032-RiboJ-CI
creator: He Shuaixin
created: 2013-09-08T11:00:00Z
modified: 2015-05-08T01:08:47Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1338_
m user id: 0
owner id: 15843
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: NahR Psel Ribo_J CI B0032
mutable notes: Pseudomonas putida, phiR73, E.coli
mutable provenance: lab made
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: cI
sequence annotation: promoter
sequence annotation: nahR
sequence annotation: b0032
sequence: https://synbiohub.org/public/igem/BBa_K1031015_sequence/1

cI
type: SequenceAnnotation
persistent identity: annotation2354028
display id: annotation2354028
version: 1
top level: BBa_K1031015
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1031015/annotation2354028/range2354028/1

https://synbiohub.org/public/igem/BBa_K1031015/annotation2354028/range2354028/1
type: Range
persistent identity: range2354028
display id: range2354028
version: 1
direction: direction/forward
top level: BBa_K1031015
start: 1169
end: 1885
orientation: inline

promoter
type: SequenceAnnotation
persistent identity: annotation2354026
display id: annotation2354026
version: 1
top level: BBa_K1031015
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1031015/annotation2354026/range2354026/1

https://synbiohub.org/public/igem/BBa_K1031015/annotation2354026/range2354026/1
type: Range
persistent identity: range2354026
display id: range2354026
version: 1
direction: direction/forward
top level: BBa_K1031015
start: 904
end: 1050
orientation: inline

nahR
type: SequenceAnnotation
persistent identity: annotation2354025
display id: annotation2354025
version: 1
top level: BBa_K1031015
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1031015/annotation2354025/range2354025/1

https://synbiohub.org/public/igem/BBa_K1031015/annotation2354025/range2354025/1
type: Range
persistent identity: range2354025
display id: range2354025
version: 1
direction: direction/reverse
top level: BBa_K1031015
start: 1
end: 903
orientation: inline

b0032
type: SequenceAnnotation
persistent identity: annotation2354027
display id: annotation2354027
version: 1
top level: BBa_K1031015
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1031015/annotation2354027/range2354027/1

https://synbiohub.org/public/igem/BBa_K1031015/annotation2354027/range2354027/1
type: Range
persistent identity: range2354027
display id: range2354027
version: 1
direction: direction/forward
top level: BBa_K1031015
start: 1150
end: 1162
orientation: inline

penI
type: ComponentDefinition
persistent identity: BBa C0074
display id: BBa_C0074
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: penI repressor from Bacillus licheniformis (+LVA)
creator: crackdots
created: 2004-01-27T12:00:00Z
modified: 2015-08-31T04:07:23Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: -- No description --
mutable notes: Since penI is being ported from Bacillus Licheniformis, the sequence was changed within the coding region to reflect codon usage in E.Coli K12. Codons were mapped to the codon of the same usage rank in E.Coli.
mutable provenance: bacillus licheniformis
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: penI
sequence annotation: LVA
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C0074_sequence/1

penI
type: SequenceAnnotation
persistent identity: annotation302638
display id: annotation302638
version: 1
top level: penI
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_C0074/annotation302638/range302638/1

https://synbiohub.org/public/igem/BBa_C0074/annotation302638/range302638/1
type: Range
persistent identity: range302638
display id: range302638
version: 1
direction: direction/forward
top level: penI
start: 1
end: 387
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation306563
display id: annotation306563
version: 1
top level: penI
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_C0074/annotation306563/range306563/1

https://synbiohub.org/public/igem/BBa_C0074/annotation306563/range306563/1
type: Range
persistent identity: range306563
display id: range306563
version: 1
direction: direction/forward
top level: penI
start: 385
end: 417
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2214009
display id: annotation2214009
version: 1
top level: penI
role: SO:0000807
role: feature/barcode
location: https://synbiohub.org/public/igem/BBa_C0074/annotation2214009/range2214009/1

https://synbiohub.org/public/igem/BBa_C0074/annotation2214009/range2214009/1
type: Range
persistent identity: range2214009
display id: range2214009
version: 1
direction: direction/forward
top level: penI
start: 424
end: 448
orientation: inline

BBa_K1520505
type: ComponentDefinition
persistent identity: BBa K1520505
display id: BBa_K1520505
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Rbs-tetR(scarless)
creator: yue wu
created: 2014-09-21T11:00:00Z
modified: 2015-05-08T01:10:49Z
discontinued: false
dominant: false
group u list: _1902_
m user id: 0
owner id: 21318
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Rbs-tetR(scarless) It is an improvement of tetR(BBa_C0040) by adding a rbs.
mutable notes: None
mutable provenance: It is an improvement of tetR(BBa_C0040) by adding a rbs.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
sequence annotation: rbs
sequence annotation: tetR
sequence: https://synbiohub.org/public/igem/BBa_K1520505_sequence/1

rbs
type: SequenceAnnotation
persistent identity: annotation2384877
display id: annotation2384877
version: 1
top level: BBa_K1520505
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1520505/annotation2384877/range2384877/1

https://synbiohub.org/public/igem/BBa_K1520505/annotation2384877/range2384877/1
type: Range
persistent identity: range2384877
display id: range2384877
version: 1
direction: direction/forward
top level: BBa_K1520505
start: 1
end: 18
orientation: inline

tetR
type: SequenceAnnotation
persistent identity: annotation2384878
display id: annotation2384878
version: 1
top level: BBa_K1520505
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1520505/annotation2384878/range2384878/1

https://synbiohub.org/public/igem/BBa_K1520505/annotation2384878/range2384878/1
type: Range
persistent identity: range2384878
display id: range2384878
version: 1
direction: direction/forward
top level: BBa_K1520505
start: 19
end: 703
orientation: inline

BBa_K1031710
type: ComponentDefinition
persistent identity: BBa K1031710
display id: BBa_K1031710
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PaaX-Terminator
creator: He Shuaixin
created: 2013-09-08T11:00:00Z
modified: 2015-05-08T01:08:47Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1338_
m user id: 0
owner id: 15843
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: PaaX-TT
mutable notes: Molecular cloning
mutable provenance: PCR genome E.coli
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: PaaX
sequence annotation: BBa_B0034
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_K1031710_sequence/1

PaaX
type: SequenceAnnotation
persistent identity: annotation2357486
display id: annotation2357486
version: 1
top level: BBa_K1031710
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1031710/annotation2357486/range2357486/1

https://synbiohub.org/public/igem/BBa_K1031710/annotation2357486/range2357486/1
type: Range
persistent identity: range2357486
display id: range2357486
version: 1
direction: direction/forward
top level: BBa_K1031710
start: 19
end: 969
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2357485
display id: annotation2357485
version: 1
top level: BBa_K1031710
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1031710/annotation2357485/range2357485/1

https://synbiohub.org/public/igem/BBa_K1031710/annotation2357485/range2357485/1
type: Range
persistent identity: range2357485
display id: range2357485
version: 1
direction: direction/forward
top level: BBa_K1031710
start: 1
end: 12
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2357487
display id: annotation2357487
version: 1
top level: BBa_K1031710
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1031710/annotation2357487/range2357487/1

https://synbiohub.org/public/igem/BBa_K1031710/annotation2357487/range2357487/1
type: Range
persistent identity: range2357487
display id: range2357487
version: 1
direction: direction/forward
top level: BBa_K1031710
start: 978
end: 1106
orientation: inline

BBa_K1031510
type: ComponentDefinition
persistent identity: BBa K1031510
display id: BBa_K1031510
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: HpaR-Terminator
creator: He Shuaixin
created: 2013-09-08T11:00:00Z
modified: 2015-05-08T01:08:47Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1338_
m user id: 0
owner id: 15843
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: HpaR-TT
mutable notes: molecular cloning
mutable provenance: PCR genome E.coli
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: BBa_B0015
sequence annotation: HpaR
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K1031510_sequence/1

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2357473
display id: annotation2357473
version: 1
top level: BBa_K1031510
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1031510/annotation2357473/range2357473/1

https://synbiohub.org/public/igem/BBa_K1031510/annotation2357473/range2357473/1
type: Range
persistent identity: range2357473
display id: range2357473
version: 1
direction: direction/forward
top level: BBa_K1031510
start: 474
end: 602
orientation: inline

HpaR
type: SequenceAnnotation
persistent identity: annotation2357472
display id: annotation2357472
version: 1
top level: BBa_K1031510
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1031510/annotation2357472/range2357472/1

https://synbiohub.org/public/igem/BBa_K1031510/annotation2357472/range2357472/1
type: Range
persistent identity: range2357472
display id: range2357472
version: 1
direction: direction/forward
top level: BBa_K1031510
start: 19
end: 465
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2357469
display id: annotation2357469
version: 1
top level: BBa_K1031510
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1031510/annotation2357469/range2357469/1

https://synbiohub.org/public/igem/BBa_K1031510/annotation2357469/range2357469/1
type: Range
persistent identity: range2357469
display id: range2357469
version: 1
direction: direction/forward
top level: BBa_K1031510
start: 1
end: 12
orientation: inline

BBa_K1072002
type: ComponentDefinition
persistent identity: BBa K1072002
display id: BBa_K1072002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: NADH dehydrogenase
creator: Yichong wang, Ruichen, Xihao liao and Junjie zhang
created: 2013-08-31T11:00:00Z
modified: 2015-05-08T01:09:01Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1381_
m user id: 0
owner id: 16002
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: BBa_K1072002 is the generator fo NADH dehydrogenase which is an enzyme following chemical reaction: NADH + H+ acceptor \rightleftharpoons NAD+ + reduced acceptor
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K1072001
component: BBa_R0062
component: BBa_B0010
component: BBa_B0012
component: BBa_B0034
sequence annotation: BBa_K1072001
sequence annotation: BBa_R0062
sequence annotation: BBa_B0012
sequence annotation: BBa_B0034
sequence annotation: BBa_B0010
sequence: https://synbiohub.org/public/igem/BBa_K1072002_sequence/1

BBa_K1072001
type: Component
persistent identity: component2334352
display id: component2334352
version: 1
top level: BBa_K1072002
access: public
definition: https://synbiohub.org/public/igem/BBa_K1072001/1

BBa_R0062
type: Component
persistent identity: component2334344
display id: component2334344
version: 1
top level: BBa_K1072002
access: public
definition: https://synbiohub.org/public/igem/BBa_R0062/1

BBa_B0010
type: Component
persistent identity: component2334353
display id: component2334353
version: 1
top level: BBa_K1072002
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0012
type: Component
persistent identity: component2334355
display id: component2334355
version: 1
top level: BBa_K1072002
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0034
type: Component
persistent identity: component2334350
display id: component2334350
version: 1
top level: BBa_K1072002
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K1072001
type: SequenceAnnotation
persistent identity: annotation2334352
display id: annotation2334352
version: 1
top level: BBa_K1072002
location: https://synbiohub.org/public/igem/BBa_K1072002/annotation2334352/range2334352/1
component: BBa_K1072001

https://synbiohub.org/public/igem/BBa_K1072002/annotation2334352/range2334352/1
type: Range
persistent identity: range2334352
display id: range2334352
version: 1
direction: direction/reverse
top level: BBa_K1072002
start: 84
end: 1388
orientation: inline

BBa_R0062
type: SequenceAnnotation
persistent identity: annotation2334344
display id: annotation2334344
version: 1
top level: BBa_K1072002
location: https://synbiohub.org/public/igem/BBa_K1072002/annotation2334344/range2334344/1
component: BBa_R0062

https://synbiohub.org/public/igem/BBa_K1072002/annotation2334344/range2334344/1
type: Range
persistent identity: range2334344
display id: range2334344
version: 1
direction: direction/forward
top level: BBa_K1072002
start: 1
end: 55
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2334355
display id: annotation2334355
version: 1
top level: BBa_K1072002
location: https://synbiohub.org/public/igem/BBa_K1072002/annotation2334355/range2334355/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K1072002/annotation2334355/range2334355/1
type: Range
persistent identity: range2334355
display id: range2334355
version: 1
direction: direction/forward
top level: BBa_K1072002
start: 1485
end: 1525
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2334350
display id: annotation2334350
version: 1
top level: BBa_K1072002
location: https://synbiohub.org/public/igem/BBa_K1072002/annotation2334350/range2334350/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1072002/annotation2334350/range2334350/1
type: Range
persistent identity: range2334350
display id: range2334350
version: 1
direction: direction/reverse
top level: BBa_K1072002
start: 64
end: 75
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2334353
display id: annotation2334353
version: 1
top level: BBa_K1072002
location: https://synbiohub.org/public/igem/BBa_K1072002/annotation2334353/range2334353/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K1072002/annotation2334353/range2334353/1
type: Range
persistent identity: range2334353
display id: range2334353
version: 1
direction: direction/forward
top level: BBa_K1072002
start: 1397
end: 1476
orientation: inline

BBa_K1520015
type: ComponentDefinition
persistent identity: BBa K1520015
display id: BBa_K1520015
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Plac-rbs-tetR-Ter
creator: yue wu
created: 2014-09-21T11:00:00Z
modified: 2015-05-08T01:10:49Z
discontinued: false
dominant: false
group u list: _1902_
m user id: 0
owner id: 21318
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Plac-rbs-tetR-Ter. It produces tetR when there is enough IPTG or less lacI.
mutable notes: None
mutable provenance: Plac, tetR, Ter
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1520505
component: BBa_R0010
component: BBa_K1520029
sequence annotation: BBa_R0010
sequence annotation: BBa_K1520029
sequence annotation: BBa_K1520505
sequence: https://synbiohub.org/public/igem/BBa_K1520015_sequence/1

BBa_K1520505
type: Component
persistent identity: component2385008
display id: component2385008
version: 1
top level: BBa_K1520015
access: public
definition: BBa_K1520505

BBa_R0010
type: Component
persistent identity: component2384999
display id: component2384999
version: 1
top level: BBa_K1520015
access: public
definition: https://synbiohub.org/public/igem/BBa_R0010/1

BBa_K1520029
type: Component
persistent identity: component2385009
display id: component2385009
version: 1
top level: BBa_K1520015
access: public
definition: https://synbiohub.org/public/igem/BBa_K1520029/1

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation2384999
display id: annotation2384999
version: 1
top level: BBa_K1520015
location: https://synbiohub.org/public/igem/BBa_K1520015/annotation2384999/range2384999/1
component: BBa_R0010

https://synbiohub.org/public/igem/BBa_K1520015/annotation2384999/range2384999/1
type: Range
persistent identity: range2384999
display id: range2384999
version: 1
direction: direction/forward
top level: BBa_K1520015
start: 1
end: 200
orientation: inline

BBa_K1520029
type: SequenceAnnotation
persistent identity: annotation2385009
display id: annotation2385009
version: 1
top level: BBa_K1520015
location: https://synbiohub.org/public/igem/BBa_K1520015/annotation2385009/range2385009/1
component: BBa_K1520029

https://synbiohub.org/public/igem/BBa_K1520015/annotation2385009/range2385009/1
type: Range
persistent identity: range2385009
display id: range2385009
version: 1
direction: direction/reverse
top level: BBa_K1520015
start: 920
end: 1048
orientation: inline

BBa_K1520505
type: SequenceAnnotation
persistent identity: annotation2385008
display id: annotation2385008
version: 1
top level: BBa_K1520015
location: https://synbiohub.org/public/igem/BBa_K1520015/annotation2385008/range2385008/1
component: BBa_K1520505

https://synbiohub.org/public/igem/BBa_K1520015/annotation2385008/range2385008/1
type: Range
persistent identity: range2385008
display id: range2385008
version: 1
direction: direction/reverse
top level: BBa_K1520015
start: 209
end: 911
orientation: inline

BBa_K1520004
type: ComponentDefinition
persistent identity: BBa K1520004
display id: BBa_K1520004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Prlux-rbs-lacI-Ter
creator: yue wu
created: 2014-09-21T11:00:00Z
modified: 2015-05-08T01:10:48Z
discontinued: false
dominant: false
group u list: _1902_
m user id: 0
owner id: 21318
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Prlux-rbs-lacI-Ter. It worked out an expression of lacI in the presence of luxR and AHL.
mutable notes: None
mutable provenance: Prlux, lacI, Ter
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_R0062
component: BBa_K1520029
component: BBa_K1520502
sequence annotation: BBa_R0062
sequence annotation: BBa_K1520502
sequence annotation: BBa_K1520029
sequence: https://synbiohub.org/public/igem/BBa_K1520004_sequence/1

BBa_R0062
type: Component
persistent identity: component2384890
display id: component2384890
version: 1
top level: BBa_K1520004
access: public
definition: https://synbiohub.org/public/igem/BBa_R0062/1

BBa_K1520029
type: Component
persistent identity: component2384898
display id: component2384898
version: 1
top level: BBa_K1520004
access: public
definition: https://synbiohub.org/public/igem/BBa_K1520029/1

BBa_K1520502
type: Component
persistent identity: component2384897
display id: component2384897
version: 1
top level: BBa_K1520004
access: public
definition: BBa_K1520502

BBa_R0062
type: SequenceAnnotation
persistent identity: annotation2384890
display id: annotation2384890
version: 1
top level: BBa_K1520004
location: https://synbiohub.org/public/igem/BBa_K1520004/annotation2384890/range2384890/1
component: BBa_R0062

https://synbiohub.org/public/igem/BBa_K1520004/annotation2384890/range2384890/1
type: Range
persistent identity: range2384890
display id: range2384890
version: 1
direction: direction/forward
top level: BBa_K1520004
start: 1
end: 55
orientation: inline

BBa_K1520502
type: SequenceAnnotation
persistent identity: annotation2384897
display id: annotation2384897
version: 1
top level: BBa_K1520004
location: https://synbiohub.org/public/igem/BBa_K1520004/annotation2384897/range2384897/1
component: BBa_K1520502

https://synbiohub.org/public/igem/BBa_K1520004/annotation2384897/range2384897/1
type: Range
persistent identity: range2384897
display id: range2384897
version: 1
direction: direction/reverse
top level: BBa_K1520004
start: 64
end: 1234
orientation: inline

BBa_K1520029
type: SequenceAnnotation
persistent identity: annotation2384898
display id: annotation2384898
version: 1
top level: BBa_K1520004
location: https://synbiohub.org/public/igem/BBa_K1520004/annotation2384898/range2384898/1
component: BBa_K1520029

https://synbiohub.org/public/igem/BBa_K1520004/annotation2384898/range2384898/1
type: Range
persistent identity: range2384898
display id: range2384898
version: 1
direction: direction/reverse
top level: BBa_K1520004
start: 1243
end: 1371
orientation: inline

BBa_K518003
type: ComponentDefinition
persistent identity: BBa K518003
display id: BBa_K518003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LexA - a repressor for LexA-regulated SOS promoters -
creator: Masato Ohgishi
created: 2011-09-14T11:00:00Z
modified: 2015-05-08T01:12:33Z
discontinued: false
dominant: false
group u list: _683_
m user id: 0
owner id: 8305
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Escherichia coli and other organisms have a gene cluster activated in case of emergency, which is called "SOS genes". It is well-documented that in E. coli, most of those SOS genes are repressed by LexA protein. When the cell is exposed to excess stress, RecA is up-produced, which promotes the self-degradation of LexA. As a result, many LexA-regulated genes become expressed. Combined with RecA () and SOS promoters (for instance, ), a stress-responsive gene-expression circuit was assembled. See ().
mutable notes: None.
mutable provenance: Cloned de novo from Escherichia coli JM109 strain.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K518003_sequence/1

ARL2A0301
type: ComponentDefinition
persistent identity: BBa I732112
display id: BBa_I732112
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: ARL2A0301
creator: Zhan Jian
created: 2007-10-13T11:00:00Z
modified: 2015-08-31T04:07:57Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_I732112_sequence/1

BBa_K292006
type: ComponentDefinition
persistent identity: BBa K292006
display id: BBa_K292006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Strong RBS + LacI repressor + double terminator
creator: David Charoy
created: 2009-10-18T11:00:00Z
modified: 2015-05-08T01:11:49Z
discontinued: false
dominant: true
group u list: _394_
m user id: 0
owner id: 5212
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part contains a strong ribosome binding site, the LacI repressor and a double terminator (BBa_B0030 + BBa_C0012 + BBa_B0014). This brick comes from the BBa_K292005. The LacI repressor represses pLac promoter from the E.coli lactose operon. So LacI repressor and this bio-brick are really reliable to use when we want to control a gene expression by using pLac promoter. In fact when LacI is not expressed, pLac is activated, and when there is an expression of LacI, pLac is inhibited. We can easily design a system to induce the transcription of LacI by using another operon as the tetracycline operon and creates a failed feedback mechanism for pLac inhibition and so, for gene expression inhibition. The LacI (BBa_K292006) already contains a strong RBS and a double terminator, so this brick is ready to use! History: In E.Coli, pLac is a part of the lactose operon. LacI is constantly expressed and represses the pLac. In the presence of Lactose, LacI is inhibited and pLac is active and allows the transcription of beta-galactosidase. Lactose can be substituted by IPTG (Isopropyl β-D-1-thiogalactopyranoside).
mutable notes: N/A
mutable provenance: Part:BBa_B0030, Designed by Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. Group: NCBS, Prather Lab Part:BBa_C0012, Designed by Grace Kenney, Daniel Shen, Neelaksh Varshney, Samantha Sutton Group: Registry Part:BBa_B0014, Designed by Reshma Shetty Group: Registry (2003-07-16)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0014
component: BBa_B0030
component: BBa_C0012
sequence annotation: BBa_C0012
sequence annotation: BBa_B0014
sequence annotation: BBa_B0030
sequence: https://synbiohub.org/public/igem/BBa_K292006_sequence/1

BBa_B0014
type: Component
persistent identity: component2249941
display id: component2249941
version: 1
top level: BBa_K292006
access: public
definition: https://synbiohub.org/public/igem/BBa_B0014/1

BBa_B0030
type: Component
persistent identity: component2249929
display id: component2249929
version: 1
top level: BBa_K292006
access: public
definition: https://synbiohub.org/public/igem/BBa_B0030/1

BBa_C0012
type: Component
persistent identity: component2249932
display id: component2249932
version: 1
top level: BBa_K292006
access: public
definition: lacI

BBa_C0012
type: SequenceAnnotation
persistent identity: annotation2249932
display id: annotation2249932
version: 1
top level: BBa_K292006
location: https://synbiohub.org/public/igem/BBa_K292006/annotation2249932/range2249932/1
component: BBa_C0012

https://synbiohub.org/public/igem/BBa_K292006/annotation2249932/range2249932/1
type: Range
persistent identity: range2249932
display id: range2249932
version: 1
direction: direction/forward
top level: BBa_K292006
start: 22
end: 1149
orientation: inline

BBa_B0014
type: SequenceAnnotation
persistent identity: annotation2249941
display id: annotation2249941
version: 1
top level: BBa_K292006
location: https://synbiohub.org/public/igem/BBa_K292006/annotation2249941/range2249941/1
component: BBa_B0014

https://synbiohub.org/public/igem/BBa_K292006/annotation2249941/range2249941/1
type: Range
persistent identity: range2249941
display id: range2249941
version: 1
direction: direction/reverse
top level: BBa_K292006
start: 1183
end: 1277
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation2249929
display id: annotation2249929
version: 1
top level: BBa_K292006
location: https://synbiohub.org/public/igem/BBa_K292006/annotation2249929/range2249929/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_K292006/annotation2249929/range2249929/1
type: Range
persistent identity: range2249929
display id: range2249929
version: 1
direction: direction/forward
top level: BBa_K292006
start: 1
end: 15
orientation: inline

LacI
type: ComponentDefinition
persistent identity: BBa K143033
display id: BBa_K143033
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LacI (Lva-, N-terminal deletion) regulatory protein
creator: Chris Hirst
created: 2008-09-15T11:00:00Z
modified: 2015-05-08T01:10:24Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _199_
m user id: 0
owner id: 3475
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: LacI is a regulatory protein responsible for the repression of many catabolite genes. Transcription is regulated by proteins which bind operator sequences around the transcription start site. These proteins can positively affect transcription (activators) or negatively affect transcription (reppresors). Some repressor proteins can be inactivted however by addition of an inducer, such as IPTG or certain sugars. LacI if the regulator protein for the lactose operon in ''E.coli'' and the hyper-spank protein of ''B. subtilis''#1(<bbpart>BBaK143015</bbpart>) and is responsible for ensuring that in the absence of lactose (or IPTG) that there is no expression trough these promoter. LacI is not endogenous to ''B. subtilis'', so LacI will need to be expressed in the host in order for the hyper-spank promoter to be regulated. In the presence of IPTG or lactose, the LacI tetramer is unable to bind DNA and so transcription resumes. This version of LacI lacks a Lva degradation tag and has a small(3 amino acid) N-terminal deletion relative to the current registry LacI (<bbpart>BBa_C0012</bbpart)> and is derivatives. The N-terminal deletion appears to be common to most of the LacI genes used in conjunction with ''B. subtilis'' though both forms are found in ''E.coli'' (in differing strains). LacI was used in conjunction with the '''Hyper-spank promoter''' (<bbpart>BBa_K143015<bbpart>) and acted as an input adaptor for a '''Polymerases per second''' (POPS) output ====References==== <biblio> #1 pmid=16166525 </biblio>
mutable notes: LacI was located in the sequence of the ''B. subtilis'' shuttle vector pDR111. This version of LacI lacks a Ltva degradation sequence and has a small N-terminal deletion that is observed in many LacI used in studies on ''B.subtilis''. In particular, this LacI protein is used in pDR111 to regulate expression of the inducible Phyper-spank protein (<bbpart>BBa_K143015</bbpart>) (also used in the pDR111 vector). The BioBrick prefix and suffix were applied to the gene
mutable provenance: The LacI gene was cloned from''B. subtilis'' shuttle vector pDR111 using Pfu DNA polymerase PCR
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: stop
sequence annotation: LacI (Lva-, N-terminal deletion) regulatory protein
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_K143033_sequence/1

stop
type: SequenceAnnotation
persistent identity: annotation1994271
display id: annotation1994271
version: 1
top level: LacI
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K143033/annotation1994271/range1994271/1

https://synbiohub.org/public/igem/BBa_K143033/annotation1994271/range1994271/1
type: Range
persistent identity: range1994271
display id: range1994271
version: 1
direction: direction/forward
top level: LacI
start: 1081
end: 1083
orientation: inline

LacI (Lva-, N-terminal deletion) regulatory protein
type: SequenceAnnotation
persistent identity: annotation1975974
display id: annotation1975974
version: 1
top level: LacI
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K143033/annotation1975974/range1975974/1

https://synbiohub.org/public/igem/BBa_K143033/annotation1975974/range1975974/1
type: Range
persistent identity: range1975974
display id: range1975974
version: 1
direction: direction/forward
top level: LacI
start: 1
end: 1080
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1994272
display id: annotation1994272
version: 1
top level: LacI
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K143033/annotation1994272/range1994272/1

https://synbiohub.org/public/igem/BBa_K143033/annotation1994272/range1994272/1
type: Range
persistent identity: range1994272
display id: range1994272
version: 1
direction: direction/forward
top level: LacI
start: 1084
end: 1086
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation1992702
display id: annotation1992702
version: 1
top level: LacI
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K143033/annotation1992702/range1992702/1

https://synbiohub.org/public/igem/BBa_K143033/annotation1992702/range1992702/1
type: Range
persistent identity: range1992702
display id: range1992702
version: 1
direction: direction/forward
top level: LacI
start: 1
end: 3
orientation: inline

lsrR
type: ComponentDefinition
persistent identity: BBa K091001
display id: BBa_K091001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LsrR gene
creator: Robert Cool
created: 2008-06-18T11:00:00Z
modified: 2015-05-08T01:08:37Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _191_
m user id: 0
owner id: 3080
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This sequence codes for the LsrR gene which is found in the E. coli strain MG1655. When this protein fors a complex with phosphorylated AI-2 it promotes the LsrA promoter.
mutable notes: no special considerations
mutable provenance: The DNA for this part is found in the E. coli strain MG1655.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K091001_sequence/1

BBa_K1520514
type: ComponentDefinition
persistent identity: BBa K1520514
display id: BBa_K1520514
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: MC31-rbs-tetR-Ter-PtetO-rbs-rfp-Ter-Plac-rbs-tetR-Ter-Pcons2-rbs-lacI-Ter
creator: yue wu
created: 2014-09-22T11:00:00Z
modified: 2015-05-08T01:10:49Z
discontinued: false
dominant: false
group u list: _1902_
m user id: 0
owner id: 21318
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: MC31-rbs-tetR-Ter-PtetO-rbs-rfp-Ter-Plac-rbs-tetR-Ter-Pcons2-rbs-lacI-Ter It is a test of our suicide circuit for microcystin.
mutable notes: None
mutable provenance: MC31, tetR, PtetO, rfp, Plac, Pcons2, lacI, Ter
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1520505
component: BBa_K1520002
component: BBa_K1520026
sequence annotation: BBa_K1520026
sequence annotation: BBa_K1520505
sequence annotation: BBa_K1520002
sequence: https://synbiohub.org/public/igem/BBa_K1520514_sequence/1

BBa_K1520505
type: Component
persistent identity: component2385636
display id: component2385636
version: 1
top level: BBa_K1520514
access: public
definition: BBa_K1520505

BBa_K1520002
type: Component
persistent identity: component2385633
display id: component2385633
version: 1
top level: BBa_K1520514
access: public
definition: https://synbiohub.org/public/igem/BBa_K1520002/1

BBa_K1520026
type: Component
persistent identity: component2385664
display id: component2385664
version: 1
top level: BBa_K1520514
access: public
definition: https://synbiohub.org/public/igem/BBa_K1520026/1

BBa_K1520026
type: SequenceAnnotation
persistent identity: annotation2385664
display id: annotation2385664
version: 1
top level: BBa_K1520514
location: https://synbiohub.org/public/igem/BBa_K1520514/annotation2385664/range2385664/1
component: BBa_K1520026

https://synbiohub.org/public/igem/BBa_K1520514/annotation2385664/range2385664/1
type: Range
persistent identity: range2385664
display id: range2385664
version: 1
direction: direction/reverse
top level: BBa_K1520514
start: 852
end: 4197
orientation: inline

BBa_K1520505
type: SequenceAnnotation
persistent identity: annotation2385636
display id: annotation2385636
version: 1
top level: BBa_K1520514
location: https://synbiohub.org/public/igem/BBa_K1520514/annotation2385636/range2385636/1
component: BBa_K1520505

https://synbiohub.org/public/igem/BBa_K1520514/annotation2385636/range2385636/1
type: Range
persistent identity: range2385636
display id: range2385636
version: 1
direction: direction/reverse
top level: BBa_K1520514
start: 141
end: 843
orientation: inline

BBa_K1520002
type: SequenceAnnotation
persistent identity: annotation2385633
display id: annotation2385633
version: 1
top level: BBa_K1520514
location: https://synbiohub.org/public/igem/BBa_K1520514/annotation2385633/range2385633/1
component: BBa_K1520002

https://synbiohub.org/public/igem/BBa_K1520514/annotation2385633/range2385633/1
type: Range
persistent identity: range2385633
display id: range2385633
version: 1
direction: direction/reverse
top level: BBa_K1520514
start: 1
end: 132
orientation: inline

cI 434
type: ComponentDefinition
persistent identity: BBa C0052
display id: BBa_C0052
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: cI repressor from phage 434 (+LVA)
creator: Maia Mahoney
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:23Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: The 434 cI repressor protein coding sequence is a 710 base-pair sequence with the standard RBS-compatible BioBrick prefix and the standard BioBrick suffix sections on its ends. It binds to the 434 regulatory sequence, BBa_R0052. The sequence contains a LVA tag for faster degredation and has no RBS.
mutable notes: References (unparsed) here: Nikolnikov,S., Posfai,G. and Sain,B. The construction of a versatile plasmid vector that allows direct selection of fragments cloned into six unique sites of the cI gene of coliphage 434. Gene 30 (1-3), 261-265 (1984) References (unparsed) here: Nikolnikov,S., Posfai,G. and Sain,B. The construction of a versatile plasmid vector that allows direct selection of fragments cloned into six unique sites of the cI gene of coliphage 434. Gene 30 (1-3), 261-265 (1984)
mutable provenance: Bacteriophage 434
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: BBa_C0052
sequence annotation: Help:Barcodes
sequence annotation: cI 434
sequence annotation: LVA
sequence: https://synbiohub.org/public/igem/BBa_C0052_sequence/1

BBa_C0052
type: SequenceAnnotation
persistent identity: annotation7035
display id: annotation7035
version: 1
top level: cI 434
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_C0052/annotation7035/range7035/1

https://synbiohub.org/public/igem/BBa_C0052/annotation7035/range7035/1
type: Range
persistent identity: range7035
display id: range7035
version: 1
direction: direction/forward
top level: cI 434
start: 1
end: 669
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213992
display id: annotation2213992
version: 1
top level: cI 434
role: SO:0000807
role: feature/barcode
location: https://synbiohub.org/public/igem/BBa_C0052/annotation2213992/range2213992/1

https://synbiohub.org/public/igem/BBa_C0052/annotation2213992/range2213992/1
type: Range
persistent identity: range2213992
display id: range2213992
version: 1
direction: direction/forward
top level: cI 434
start: 670
end: 694
orientation: inline

cI 434
type: SequenceAnnotation
persistent identity: annotation1743
display id: annotation1743
version: 1
top level: cI 434
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_C0052/annotation1743/range1743/1

https://synbiohub.org/public/igem/BBa_C0052/annotation1743/range1743/1
type: Range
persistent identity: range1743
display id: range1743
version: 1
direction: direction/forward
top level: cI 434
start: 1
end: 669
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation1745
display id: annotation1745
version: 1
top level: cI 434
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_C0052/annotation1745/range1745/1

https://synbiohub.org/public/igem/BBa_C0052/annotation1745/range1745/1
type: Range
persistent identity: range1745
display id: range1745
version: 1
direction: direction/forward
top level: cI 434
start: 631
end: 669
orientation: inline

BBa_K1520017
type: ComponentDefinition
persistent identity: BBa K1520017
display id: BBa_K1520017
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Pcons2-rbs-luxR-Ter-Prlux-rbs-lacI-Ter-Plac-rbs-rfp-Ter
creator: yue wu
created: 2014-09-21T11:00:00Z
modified: 2015-05-08T01:10:49Z
discontinued: false
dominant: false
group u list: _1902_
m user id: 0
owner id: 21318
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Plasmid1 in detector: Pcons2-rbs-luxR-Ter-Prlux-rbs-lacI-Ter-Plac-rbs-rfp-Ter It is a high level detection plasmid. It produces rfp when AHL is not so much.
mutable notes: None
mutable provenance: Pcons2,luxR, Ter, Prlux, lacI, Ter, Plac, rfp, Ter
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1520016
component: BBa_K1520005
sequence annotation: BBa_K1520016
sequence annotation: BBa_K1520005
sequence: https://synbiohub.org/public/igem/BBa_K1520017_sequence/1

BBa_K1520016
type: Component
persistent identity: component2385042
display id: component2385042
version: 1
top level: BBa_K1520017
access: public
definition: BBa_K1520016

BBa_K1520005
type: Component
persistent identity: component2385052
display id: component2385052
version: 1
top level: BBa_K1520017
access: public
definition: https://synbiohub.org/public/igem/BBa_K1520005/1

BBa_K1520016
type: SequenceAnnotation
persistent identity: annotation2385042
display id: annotation2385042
version: 1
top level: BBa_K1520017
location: https://synbiohub.org/public/igem/BBa_K1520017/annotation2385042/range2385042/1
component: BBa_K1520016

https://synbiohub.org/public/igem/BBa_K1520017/annotation2385042/range2385042/1
type: Range
persistent identity: range2385042
display id: range2385042
version: 1
direction: direction/reverse
top level: BBa_K1520017
start: 1
end: 2358
orientation: inline

BBa_K1520005
type: SequenceAnnotation
persistent identity: annotation2385052
display id: annotation2385052
version: 1
top level: BBa_K1520017
location: https://synbiohub.org/public/igem/BBa_K1520017/annotation2385052/range2385052/1
component: BBa_K1520005

https://synbiohub.org/public/igem/BBa_K1520017/annotation2385052/range2385052/1
type: Range
persistent identity: range2385052
display id: range2385052
version: 1
direction: direction/reverse
top level: BBa_K1520017
start: 2367
end: 3443
orientation: inline

BBa_C0056
type: ComponentDefinition
persistent identity: BBa C0056
display id: BBa_C0056
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: cI repressor from phage 434 (no LVA)
creator: Reshma Shetty
created: 2004-09-01T11:00:00Z
modified: 2015-08-31T04:07:23Z
discontinued: false
dominant: false
experience: experience/None
group u list: _41_6_
m user id: 0
owner id: 126
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: -- No description --
mutable notes: Removed LVA tag from BBa_C0052 via PCR. In pSB1AK3 currently.
mutable provenance: Removed LVA tag from BBa_C0052
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: cI 434
sequence annotation: 2
sequence: https://synbiohub.org/public/igem/BBa_C0056_sequence/1

cI 434
type: SequenceAnnotation
persistent identity: annotation1063931
display id: annotation1063931
version: 1
top level: BBa_C0056
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_C0056/annotation1063931/range1063931/1

https://synbiohub.org/public/igem/BBa_C0056/annotation1063931/range1063931/1
type: Range
persistent identity: range1063931
display id: range1063931
version: 1
direction: direction/forward
top level: BBa_C0056
start: 1
end: 636
orientation: inline

2
type: SequenceAnnotation
persistent identity: annotation1063930
display id: annotation1063930
version: 1
top level: BBa_C0056
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_C0056/annotation1063930/range1063930/1

https://synbiohub.org/public/igem/BBa_C0056/annotation1063930/range1063930/1
type: Range
persistent identity: range1063930
display id: range1063930
version: 1
direction: direction/forward
top level: BBa_C0056
start: 631
end: 636
orientation: inline

BBa_K544002
type: ComponentDefinition
persistent identity: BBa K544002
display id: BBa_K544002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Tetracycline Repressor with Consitutive Promoter BBa_J23109
creator: Leung Man Hong
created: 2011-09-29T11:00:00Z
modified: 2015-05-08T01:12:39Z
discontinued: false
dominant: false
experience: experience/None
group u list: _711_
m user id: 0
owner id: 6780
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: A transcriptional regulator TetR (BBa_C0040) is driven by constitutive promoter family members (BBa-J23103, BBa-J23109, BBa-J23116, and BBa-J23106), which can be used to tune the expression level of constitutively expressed TetR.
mutable notes: The SsrA site at the end of BBa_C0040 is not present in our design.
mutable provenance: The source of this part is based on the registry distribution
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: cds
sequence annotation: BBa_J23109
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K544002_sequence/1

cds
type: SequenceAnnotation
persistent identity: annotation2147988
display id: annotation2147988
version: 1
top level: BBa_K544002
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K544002/annotation2147988/range2147988/1

https://synbiohub.org/public/igem/BBa_K544002/annotation2147988/range2147988/1
type: Range
persistent identity: range2147988
display id: range2147988
version: 1
direction: direction/forward
top level: BBa_K544002
start: 62
end: 682
orientation: inline

BBa_J23109
type: SequenceAnnotation
persistent identity: annotation2147986
display id: annotation2147986
version: 1
top level: BBa_K544002
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K544002/annotation2147986/range2147986/1

https://synbiohub.org/public/igem/BBa_K544002/annotation2147986/range2147986/1
type: Range
persistent identity: range2147986
display id: range2147986
version: 1
direction: direction/forward
top level: BBa_K544002
start: 1
end: 35
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2147987
display id: annotation2147987
version: 1
top level: BBa_K544002
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K544002/annotation2147987/range2147987/1

https://synbiohub.org/public/igem/BBa_K544002/annotation2147987/range2147987/1
type: Range
persistent identity: range2147987
display id: range2147987
version: 1
direction: direction/forward
top level: BBa_K544002
start: 44
end: 55
orientation: inline

mnt (w)
type: ComponentDefinition
persistent identity: BBa C0073
display id: BBa_C0073
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: mnt repressor (weak) from Salmonella phage P22 (+LVA)
creator: crackdots
created: 2004-01-27T12:00:00Z
modified: 2015-08-31T04:07:23Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: -- No description --
mutable notes: dimeric repressor from enterobacteriophage p22 with dissociation constant of 3*10^-8. sequence was translated with the E.Coli K12 codon usage table, and nucleotides 95-97 was changed from AAT to AAC to avoid the cut site GAATTC (94-99). the mutation R10->K10 (ctg->aaa) decreases the binding affinity of the mnt protein to its operator by approximately 12,000 fold (Kd: 2.5e-12 to 3e-8)
mutable provenance: enterobacteriophage p22
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Help:Barcodes
sequence annotation: Arg->Lys (ctg)
sequence annotation: LVA
sequence annotation: T
sequence annotation: mnt
sequence: https://synbiohub.org/public/igem/BBa_C0073_sequence/1

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2214006
display id: annotation2214006
version: 1
top level: mnt (w)
role: SO:0000807
role: feature/barcode
location: https://synbiohub.org/public/igem/BBa_C0073/annotation2214006/range2214006/1

https://synbiohub.org/public/igem/BBa_C0073/annotation2214006/range2214006/1
type: Range
persistent identity: range2214006
display id: range2214006
version: 1
direction: direction/forward
top level: mnt (w)
start: 289
end: 313
orientation: inline

Arg->Lys (ctg)
type: SequenceAnnotation
persistent identity: annotation308213
display id: annotation308213
version: 1
top level: mnt (w)
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_C0073/annotation308213/range308213/1

https://synbiohub.org/public/igem/BBa_C0073/annotation308213/range308213/1
type: Range
persistent identity: range308213
display id: range308213
version: 1
direction: direction/forward
top level: mnt (w)
start: 31
end: 33
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation306535
display id: annotation306535
version: 1
top level: mnt (w)
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_C0073/annotation306535/range306535/1

https://synbiohub.org/public/igem/BBa_C0073/annotation306535/range306535/1
type: Range
persistent identity: range306535
display id: range306535
version: 1
direction: direction/forward
top level: mnt (w)
start: 250
end: 282
orientation: inline

T
type: SequenceAnnotation
persistent identity: annotation308457
display id: annotation308457
version: 1
top level: mnt (w)
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_C0073/annotation308457/range308457/1

https://synbiohub.org/public/igem/BBa_C0073/annotation308457/range308457/1
type: Range
persistent identity: range308457
display id: range308457
version: 1
direction: direction/forward
top level: mnt (w)
start: 95
end: 97
orientation: inline

mnt
type: SequenceAnnotation
persistent identity: annotation302631
display id: annotation302631
version: 1
top level: mnt (w)
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_C0073/annotation302631/range302631/1

https://synbiohub.org/public/igem/BBa_C0073/annotation302631/range302631/1
type: Range
persistent identity: range302631
display id: range302631
version: 1
direction: direction/forward
top level: mnt (w)
start: 1
end: 249
orientation: inline

Fur
type: ComponentDefinition
persistent identity: BBa K1122666
display id: BBa_K1122666
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Ferric uptake repressor
creator: Hugo Villanueva
created: 2013-07-23T11:00:00Z
modified: 2015-07-24T01:14:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1433_
m user id: 4206
owner id: 18022
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: The ferric uptake repressor of Bacillus subtilis called YqkL is a regulator that when in contact with iron will bind and repress a regulatory sequence (the Fur box).
mutable notes: We sequenced the Fur box. This can be added in front of genes to provide regulation when in contact with iron.
mutable provenance: This comes from Bacillus subtilis.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1122666_sequence/1

BBa_K544003
type: ComponentDefinition
persistent identity: BBa K544003
display id: BBa_K544003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Tetracycline Repressor with Consitutive Promoter BBa_J23116
creator: Leung Man Hong
created: 2011-09-29T11:00:00Z
modified: 2015-05-08T01:12:39Z
discontinued: false
dominant: false
experience: experience/None
group u list: _711_
m user id: 0
owner id: 6780
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: The tetR-HNS2-90 protein fuses the polymerization demain of histone-like nucleoid-structuring (H-NS) protein to tetR. Thus, in E.coli, when TetR-HNS2-90 binds the TetR-specific DNA motif, it will recruit native H-NS to bind to adjacent DNA, which may lead to local gene silencing. This fusion protein is driven by constitutive promoter family members (BBa-J23103, BBa-J23109, BBa-J23116, and BBa-J23106). Experimental data indicates that TetR-HNS2-90 is more effective than TetR on transcription repression.
mutable notes: The source of this part is based on the registry distribution
mutable provenance: The source of this part is based on the parts in the registry distribution
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: tet-R
sequence annotation: BBa_J23116
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K544003_sequence/1

tet-R
type: SequenceAnnotation
persistent identity: annotation2147991
display id: annotation2147991
version: 1
top level: BBa_K544003
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K544003/annotation2147991/range2147991/1

https://synbiohub.org/public/igem/BBa_K544003/annotation2147991/range2147991/1
type: Range
persistent identity: range2147991
display id: range2147991
version: 1
direction: direction/forward
top level: BBa_K544003
start: 62
end: 682
orientation: inline

BBa_J23116
type: SequenceAnnotation
persistent identity: annotation2147989
display id: annotation2147989
version: 1
top level: BBa_K544003
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K544003/annotation2147989/range2147989/1

https://synbiohub.org/public/igem/BBa_K544003/annotation2147989/range2147989/1
type: Range
persistent identity: range2147989
display id: range2147989
version: 1
direction: direction/forward
top level: BBa_K544003
start: 1
end: 35
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2147990
display id: annotation2147990
version: 1
top level: BBa_K544003
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K544003/annotation2147990/range2147990/1

https://synbiohub.org/public/igem/BBa_K544003/annotation2147990/range2147990/1
type: Range
persistent identity: range2147990
display id: range2147990
version: 1
direction: direction/forward
top level: BBa_K544003
start: 44
end: 55
orientation: inline

BBa_K2056001
type: ComponentDefinition
persistent identity: BBa K2056001
display id: BBa_K2056001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: NsrR - NO(x) sensitive pYear, pNirk repressor protein
creator: Muhammad Ismail
created: 2016-10-12T11:00:00Z
modified: 2016-10-13T10:04:47Z
discontinued: false
dominant: false
group u list: _2524_
m user id: 33019
owner id: 33019
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This part contains the NsrR (NO(x) sensitive pYear, pNirk repressor protein) encoding region. Escherichia coli (E. coli) detects environmental nitrate by the yeaR-yoaG operon. PyeaR is regulated by the NsrR repressor protein. Nitric oxide binds to the NsrR protein and relieves the repression on PyeaR. As a result, any genes that are downstream of PyeaR are expressed. This part is also related with BBa_K2056002, the promoter region of the NirK protein, because NsrR activates the transcription of NirK under high nitrite conditions. Due to these properties, we can use this part as a sensor for oxides of nitrogen.
mutable notes: Because the part Bba_K1682011 was not available from the registry, we had to get it synthesized. We had to codon optimized this part to conform to assembly standards and the requirements for synthesis.
mutable provenance: This part has been designed after the part Bba_K1682011 in the Registry of Standard Biological Parts.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K2056001_sequence/1

tetR
type: ComponentDefinition
persistent identity: BBa C0040
display id: BBa_C0040
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: tetracycline repressor from transposon Tn10 (+LVA)
creator: June Rhee, Connie Tao, Ty Thomson, Louis Waldman.
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:23Z
discontinued: false
dominant: true
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Coding region for the TetR protein without the Ribosome Binding Site. Modified with an LVA tail for rapid degradation of the protein and faster fall time for the emission. TetR binds to the pTet regulator (BBa_R0040). aTc (anhydrotetracycline) binds to TetR and inhibits its operation.
mutable notes: References (unparsed) here: Elowitz, M. B. Transport, Assembly, and Dynamics in Systems of Interacting Proteins. Thesis, Princeton Univ., Princeton (1999). Lutz R, Bujard H., Independent and tight regulation of transcriptional units in Escherichia coli via the LacR/O, the TetR/O and AraC/I1-I2 regulatory elements. Nucleic Acids Res. 1997 Mar 15;25(6):1203-10. PMID: 9092630 References (unparsed) here: Elowitz, M. B. Transport, Assembly, and Dynamics in Systems of Interacting Proteins. Thesis, Princeton Univ., Princeton (1999). Lutz R, Bujard H., Independent and tight regulation of transcriptional units in Escherichia coli via the LacR/O, the TetR/O and AraC/I1-I2 regulatory elements. Nucleic Acids Res. 1997 Mar 15;25(6):1203-10. PMID: 9092630 BBa_C0040 TetR Protein is based on the TetR sequence from Elowitz's repressilator. It has been modified to include a rapid degradation LVA tail, and includes the BioBrick standard assembly head and tail restriction sites. The RBS has been removed. The stop codon has been changed from TAA to a double stop codon TAATAA.
mutable provenance: Elowitz, M. B. Transport, Assembly, and Dynamics in Systems of Interacting Proteins. Thesis, Princeton Univ., Princeton (1999)
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: SsrA
sequence annotation: Help:Barcodes
sequence annotation: tetR
sequence: https://synbiohub.org/public/igem/BBa_C0040_sequence/1

SsrA
type: SequenceAnnotation
persistent identity: annotation23330
display id: annotation23330
version: 1
top level: tetR
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_C0040/annotation23330/range23330/1

https://synbiohub.org/public/igem/BBa_C0040/annotation23330/range23330/1
type: Range
persistent identity: range23330
display id: range23330
version: 1
direction: direction/forward
top level: tetR
start: 621
end: 654
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213989
display id: annotation2213989
version: 1
top level: tetR
role: SO:0000807
role: feature/barcode
location: https://synbiohub.org/public/igem/BBa_C0040/annotation2213989/range2213989/1

https://synbiohub.org/public/igem/BBa_C0040/annotation2213989/range2213989/1
type: Range
persistent identity: range2213989
display id: range2213989
version: 1
direction: direction/forward
top level: tetR
start: 661
end: 685
orientation: inline

tetR
type: SequenceAnnotation
persistent identity: annotation23329
display id: annotation23329
version: 1
top level: tetR
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_C0040/annotation23329/range23329/1

https://synbiohub.org/public/igem/BBa_C0040/annotation23329/range23329/1
type: Range
persistent identity: range23329
display id: range23329
version: 1
direction: direction/forward
top level: tetR
start: 4
end: 620
orientation: inline

cI857
type: ComponentDefinition
persistent identity: BBa K200011
display id: BBa_K200011
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Temperature Sensitive Lamda cI Repressor
creator: Royah Vaezi, Kun Xue
created: 2009-08-30T11:00:00Z
modified: 2015-05-08T01:11:21Z
discontinued: false
dominant: false
experience: experience/None
group u list: _298_
m user id: 0
owner id: 5055
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: The temperature sensitive lambda cI protein has the cI857 mutation. Therefore, the repressor is denatured at 42??C. It normally represses promoter activity from bacteriophage lambda PL and PR promoters, and has maximal activity at the permissive temperature of 30??C. cI1 However, when the temperature is raised further, typically 42??C, the functionality of such protein is lost and it is no longer able to bind the operator of the promoter. cI2
mutable notes: Nil
mutable provenance: pGW7 plasmid from ATCC.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0034
component: BBa_B0010
component: BBa_K200016
component: BBa_B0012
sequence annotation: BBa_K200016
sequence annotation: BBa_B0012
sequence annotation: BBa_B0010
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K200011_sequence/1

BBa_B0034
type: Component
persistent identity: component2039798
display id: component2039798
version: 1
top level: cI857
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0010
type: Component
persistent identity: component2039800
display id: component2039800
version: 1
top level: cI857
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_K200016
type: Component
persistent identity: component2039799
display id: component2039799
version: 1
top level: cI857
access: public
definition: https://synbiohub.org/public/igem/BBa_K200016/1

BBa_B0012
type: Component
persistent identity: component2039802
display id: component2039802
version: 1
top level: cI857
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_K200016
type: SequenceAnnotation
persistent identity: annotation2039799
display id: annotation2039799
version: 1
top level: cI857
location: https://synbiohub.org/public/igem/BBa_K200011/annotation2039799/range2039799/1
component: BBa_K200016

https://synbiohub.org/public/igem/BBa_K200011/annotation2039799/range2039799/1
type: Range
persistent identity: range2039799
display id: range2039799
version: 1
direction: direction/reverse
top level: cI857
start: 19
end: 732
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2039802
display id: annotation2039802
version: 1
top level: cI857
location: https://synbiohub.org/public/igem/BBa_K200011/annotation2039802/range2039802/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K200011/annotation2039802/range2039802/1
type: Range
persistent identity: range2039802
display id: range2039802
version: 1
direction: direction/forward
top level: cI857
start: 829
end: 869
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2039800
display id: annotation2039800
version: 1
top level: cI857
location: https://synbiohub.org/public/igem/BBa_K200011/annotation2039800/range2039800/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K200011/annotation2039800/range2039800/1
type: Range
persistent identity: range2039800
display id: range2039800
version: 1
direction: direction/forward
top level: cI857
start: 741
end: 820
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2039798
display id: annotation2039798
version: 1
top level: cI857
location: https://synbiohub.org/public/igem/BBa_K200011/annotation2039798/range2039798/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K200011/annotation2039798/range2039798/1
type: Range
persistent identity: range2039798
display id: range2039798
version: 1
direction: direction/reverse
top level: cI857
start: 1
end: 12
orientation: inline

BBa_K1520021
type: ComponentDefinition
persistent identity: BBa K1520021
display id: BBa_K1520021
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Pcons2-rbs-lacI-Ter-Plac-rbs-luxI-Ter-Pcons2-rbs-luxR-Ter-Prlux-rbs-rfp-Ter
creator: yue wu
created: 2014-09-21T11:00:00Z
modified: 2015-05-08T01:10:49Z
discontinued: false
dominant: false
group u list: _1902_
m user id: 0
owner id: 21318
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Plasmid in positive feedback verification: Pcons2-rbs-lacI-Ter-Plac-rbs-luxI-Ter-Pcons2-rbs-luxR-Ter-Prlux-rbs-rfp-Ter. It is a control plasmid for positive feedback circuit. It will produce rfp when there is enough IPTG.
mutable notes: None
mutable provenance: Pcons2, lacI, Plac, luxI, Pcons2, luxR, Prlux, rfp, Ter
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1520020
component: BBa_K1520010
sequence annotation: BBa_K1520010
sequence annotation: BBa_K1520020
sequence: https://synbiohub.org/public/igem/BBa_K1520021_sequence/1

BBa_K1520020
type: Component
persistent identity: component2385142
display id: component2385142
version: 1
top level: BBa_K1520021
access: public
definition: BBa_K1520020

BBa_K1520010
type: Component
persistent identity: component2385150
display id: component2385150
version: 1
top level: BBa_K1520021
access: public
definition: https://synbiohub.org/public/igem/BBa_K1520010/1

BBa_K1520010
type: SequenceAnnotation
persistent identity: annotation2385150
display id: annotation2385150
version: 1
top level: BBa_K1520021
location: https://synbiohub.org/public/igem/BBa_K1520021/annotation2385150/range2385150/1
component: BBa_K1520010

https://synbiohub.org/public/igem/BBa_K1520021/annotation2385150/range2385150/1
type: Range
persistent identity: range2385150
display id: range2385150
version: 1
direction: direction/reverse
top level: BBa_K1520021
start: 3361
end: 4292
orientation: inline

BBa_K1520020
type: SequenceAnnotation
persistent identity: annotation2385142
display id: annotation2385142
version: 1
top level: BBa_K1520021
location: https://synbiohub.org/public/igem/BBa_K1520021/annotation2385142/range2385142/1
component: BBa_K1520020

https://synbiohub.org/public/igem/BBa_K1520021/annotation2385142/range2385142/1
type: Range
persistent identity: range2385142
display id: range2385142
version: 1
direction: direction/reverse
top level: BBa_K1520021
start: 1
end: 3352
orientation: inline

BBa_K822004
type: ComponentDefinition
persistent identity: BBa K822004
display id: BBa_K822004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LacI generator (no promoter)
creator: Jarle Pahr
created: 2012-09-24T11:00:00Z
modified: 2015-05-08T01:13:29Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1080_
m user id: 0
owner id: 11627
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: A composite part with coding sequence for LacI-LVA together with ribosome binding site (RBS) and a double transcriptional terminator. Fore use in regulation of LacI-repressed genetic elements, by combining the part with an appropriate promoter.
mutable notes: This part is a reconstruction of BBa_K292006. BBa_K292006 has the same theoretical sequence, but the DNA available through the registry does not match this sequence. We thus offer this part as a replacement.
mutable provenance: BioBrick parts from the Registry.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_C0012
component: BBa_B0030
component: BBa_B0014
sequence annotation: BBa_B0014
sequence annotation: BBa_B0030
sequence annotation: BBa_C0012
sequence: https://synbiohub.org/public/igem/BBa_K822004_sequence/1

BBa_C0012
type: Component
persistent identity: component2251181
display id: component2251181
version: 1
top level: BBa_K822004
access: public
definition: lacI

BBa_B0030
type: Component
persistent identity: component2251178
display id: component2251178
version: 1
top level: BBa_K822004
access: public
definition: https://synbiohub.org/public/igem/BBa_B0030/1

BBa_B0014
type: Component
persistent identity: component2251190
display id: component2251190
version: 1
top level: BBa_K822004
access: public
definition: https://synbiohub.org/public/igem/BBa_B0014/1

BBa_B0014
type: SequenceAnnotation
persistent identity: annotation2251190
display id: annotation2251190
version: 1
top level: BBa_K822004
location: https://synbiohub.org/public/igem/BBa_K822004/annotation2251190/range2251190/1
component: BBa_B0014

https://synbiohub.org/public/igem/BBa_K822004/annotation2251190/range2251190/1
type: Range
persistent identity: range2251190
display id: range2251190
version: 1
direction: direction/reverse
top level: BBa_K822004
start: 1183
end: 1277
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation2251178
display id: annotation2251178
version: 1
top level: BBa_K822004
location: https://synbiohub.org/public/igem/BBa_K822004/annotation2251178/range2251178/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_K822004/annotation2251178/range2251178/1
type: Range
persistent identity: range2251178
display id: range2251178
version: 1
direction: direction/forward
top level: BBa_K822004
start: 1
end: 15
orientation: inline

BBa_C0012
type: SequenceAnnotation
persistent identity: annotation2251181
display id: annotation2251181
version: 1
top level: BBa_K822004
location: https://synbiohub.org/public/igem/BBa_K822004/annotation2251181/range2251181/1
component: BBa_C0012

https://synbiohub.org/public/igem/BBa_K822004/annotation2251181/range2251181/1
type: Range
persistent identity: range2251181
display id: range2251181
version: 1
direction: direction/forward
top level: BBa_K822004
start: 22
end: 1149
orientation: inline

BBa_K544001
type: ComponentDefinition
persistent identity: BBa K544001
display id: BBa_K544001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Tetracycline Repressor with Consitutive Promoter BBa_J23103
creator: Leung Man Hong
created: 2011-09-29T11:00:00Z
modified: 2015-05-08T01:12:39Z
discontinued: false
dominant: false
group u list: _711_
m user id: 0
owner id: 6780
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: A transcriptional regulator TetR (BBa_C0040) is driven by constitutive promoter family members (BBa-J23103, BBa-J23109, BBa-J23116, and BBa-J23106), which can be used to tune the expression level of constitutively expressed TetR.
mutable notes: The SsrA at the end of BBa_C0040 is not present in our design.
mutable provenance: The source of this part are all from the registry distributions.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: BBa_B0034
sequence annotation: tet-R
sequence annotation: BBa_J23103
sequence: https://synbiohub.org/public/igem/BBa_K544001_sequence/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2147984
display id: annotation2147984
version: 1
top level: BBa_K544001
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K544001/annotation2147984/range2147984/1

https://synbiohub.org/public/igem/BBa_K544001/annotation2147984/range2147984/1
type: Range
persistent identity: range2147984
display id: range2147984
version: 1
direction: direction/forward
top level: BBa_K544001
start: 44
end: 55
orientation: inline

tet-R
type: SequenceAnnotation
persistent identity: annotation2147985
display id: annotation2147985
version: 1
top level: BBa_K544001
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K544001/annotation2147985/range2147985/1

https://synbiohub.org/public/igem/BBa_K544001/annotation2147985/range2147985/1
type: Range
persistent identity: range2147985
display id: range2147985
version: 1
direction: direction/forward
top level: BBa_K544001
start: 62
end: 682
orientation: inline

BBa_J23103
type: SequenceAnnotation
persistent identity: annotation2147647
display id: annotation2147647
version: 1
top level: BBa_K544001
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K544001/annotation2147647/range2147647/1

https://synbiohub.org/public/igem/BBa_K544001/annotation2147647/range2147647/1
type: Range
persistent identity: range2147647
display id: range2147647
version: 1
direction: direction/forward
top level: BBa_K544001
start: 1
end: 35
orientation: inline

lacI
type: ComponentDefinition
persistent identity: BBa C0012
display id: BBa_C0012
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: lacI repressor from E. coli (+LVA)
creator: Grace Kenney, Daniel Shen, Neelaksh Varshney, Samantha Sutton
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:23Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Coding region for the LacI protein with an LVA degradation tail and without an RBS. LacI binds to the pLac regulator <bb_part>BBa_R0010</bb_part> and PLlac01 hybrid regulator <bb_part>BBa_R0011</bb_part> and inhibits transcription. IPTG (Isopropylthiogalactoside) binds to LacI and inhibits its operation, therefore promoting transcription. A rapid degredation tail (LVA) has been added to improve the High to Low performance of this part.
mutable notes: References (unparsed) here: Elowitz, M.B., Leibler, S. A synthetic oscillatory network of transcriptional regulators. Nature 403, 335-338 (2000). [ELOW00] References (unparsed) here: Elowitz, M.B., Leibler, S. A synthetic oscillatory network of transcriptional regulators. Nature 403, 335-338 (2000). [ELOW00] Sequence taken from the repressilator of Elowitz and Leibler (2000). The obtained sequence was compared to the wild-type sequence for LacI obtained through a database search. The sequence had been modified from the wild-type in that wild-type GTG start was changed to an ATG start (note, actual ORF in E.coli has several GTG starts it would seem). The LVA tag has been added for quicker degradation. Incompatible with systems containing LacI, lactose, or IPTG.
mutable provenance: represillator of Elowitz and Leibler (2000)
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Help:Barcodes
sequence annotation: lacI-LVA
sequence annotation: BBa_C0012
sequence annotation: LVA
sequence: https://synbiohub.org/public/igem/BBa_C0012_sequence/1

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213988
display id: annotation2213988
version: 1
top level: lacI
role: SO:0000807
role: feature/barcode
location: https://synbiohub.org/public/igem/BBa_C0012/annotation2213988/range2213988/1

https://synbiohub.org/public/igem/BBa_C0012/annotation2213988/range2213988/1
type: Range
persistent identity: range2213988
display id: range2213988
version: 1
direction: direction/forward
top level: lacI
start: 1129
end: 1153
orientation: inline

lacI-LVA
type: SequenceAnnotation
persistent identity: annotation1723
display id: annotation1723
version: 1
top level: lacI
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_C0012/annotation1723/range1723/1

https://synbiohub.org/public/igem/BBa_C0012/annotation1723/range1723/1
type: Range
persistent identity: range1723
display id: range1723
version: 1
direction: direction/forward
top level: lacI
start: 1
end: 1128
orientation: inline

BBa_C0012
type: SequenceAnnotation
persistent identity: annotation7031
display id: annotation7031
version: 1
top level: lacI
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_C0012/annotation7031/range7031/1

https://synbiohub.org/public/igem/BBa_C0012/annotation7031/range7031/1
type: Range
persistent identity: range7031
display id: range7031
version: 1
direction: direction/forward
top level: lacI
start: 1
end: 1128
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation1722
display id: annotation1722
version: 1
top level: lacI
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_C0012/annotation1722/range1722/1

https://synbiohub.org/public/igem/BBa_C0012/annotation1722/range1722/1
type: Range
persistent identity: range1722
display id: range1722
version: 1
direction: direction/forward
top level: lacI
start: 1090
end: 1128
orientation: inline

BBa_K1480005
type: ComponentDefinition
persistent identity: BBa K1480005
display id: BBa_K1480005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TetR generator (Promoter J23111 )
creator: Jesus Gilberto Rodriguez Ceja
created: 2014-10-13T11:00:00Z
modified: 2015-05-08T01:10:40Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1860_
m user id: 0
owner id: 12878
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is a TetR protein generator regulated by the constitutive promoter J23111. The protein has a LVA tag for degradation.
mutable notes: It was assembled using assembly 10.
mutable provenance: Based on parts of the registry.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_J23111
component: BBa_C0040
component: BBa_B0034
component: BBa_B0010
component: BBa_B0012
sequence annotation: BBa_B0012
sequence annotation: BBa_B0034
sequence annotation: BBa_J23111
sequence annotation: BBa_C0040
sequence annotation: BBa_B0010
sequence: https://synbiohub.org/public/igem/BBa_K1480005_sequence/1

BBa_J23111
type: Component
persistent identity: component2419554
display id: component2419554
version: 1
top level: BBa_K1480005
access: public
definition: https://synbiohub.org/public/igem/BBa_J23111/1

BBa_C0040
type: Component
persistent identity: component2419560
display id: component2419560
version: 1
top level: BBa_K1480005
access: public
definition: tetR

BBa_B0034
type: Component
persistent identity: component2419556
display id: component2419556
version: 1
top level: BBa_K1480005
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0010
type: Component
persistent identity: component2419561
display id: component2419561
version: 1
top level: BBa_K1480005
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0012
type: Component
persistent identity: component2419563
display id: component2419563
version: 1
top level: BBa_K1480005
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2419563
display id: annotation2419563
version: 1
top level: BBa_K1480005
location: https://synbiohub.org/public/igem/BBa_K1480005/annotation2419563/range2419563/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K1480005/annotation2419563/range2419563/1
type: Range
persistent identity: range2419563
display id: range2419563
version: 1
direction: direction/forward
top level: BBa_K1480005
start: 843
end: 883
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2419556
display id: annotation2419556
version: 1
top level: BBa_K1480005
location: https://synbiohub.org/public/igem/BBa_K1480005/annotation2419556/range2419556/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1480005/annotation2419556/range2419556/1
type: Range
persistent identity: range2419556
display id: range2419556
version: 1
direction: direction/reverse
top level: BBa_K1480005
start: 44
end: 55
orientation: inline

BBa_J23111
type: SequenceAnnotation
persistent identity: annotation2419554
display id: annotation2419554
version: 1
top level: BBa_K1480005
location: https://synbiohub.org/public/igem/BBa_K1480005/annotation2419554/range2419554/1
component: BBa_J23111

https://synbiohub.org/public/igem/BBa_K1480005/annotation2419554/range2419554/1
type: Range
persistent identity: range2419554
display id: range2419554
version: 1
direction: direction/reverse
top level: BBa_K1480005
start: 1
end: 35
orientation: inline

BBa_C0040
type: SequenceAnnotation
persistent identity: annotation2419560
display id: annotation2419560
version: 1
top level: BBa_K1480005
location: https://synbiohub.org/public/igem/BBa_K1480005/annotation2419560/range2419560/1
component: BBa_C0040

https://synbiohub.org/public/igem/BBa_K1480005/annotation2419560/range2419560/1
type: Range
persistent identity: range2419560
display id: range2419560
version: 1
direction: direction/reverse
top level: BBa_K1480005
start: 62
end: 746
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2419561
display id: annotation2419561
version: 1
top level: BBa_K1480005
location: https://synbiohub.org/public/igem/BBa_K1480005/annotation2419561/range2419561/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K1480005/annotation2419561/range2419561/1
type: Range
persistent identity: range2419561
display id: range2419561
version: 1
direction: direction/forward
top level: BBa_K1480005
start: 755
end: 834
orientation: inline

BBa_K1520003
type: ComponentDefinition
persistent identity: BBa K1520003
display id: BBa_K1520003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Pcons2-rbs-luxR-Ter
creator: yue wu
created: 2014-09-21T11:00:00Z
modified: 2015-05-08T01:10:48Z
discontinued: false
dominant: false
group u list: _1902_
m user id: 0
owner id: 21318
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Pcons2-rbs-luxR-Ter. It achieves a constant expression of luxR, the AHL combining protein Pcons2 is BBa_J23100
mutable notes: NONE
mutable provenance: Pcons2, luxR, Ter
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_J23100
component: BBa_K1520501
component: BBa_K1520029
sequence annotation: BBa_K1520501
sequence annotation: BBa_K1520029
sequence annotation: BBa_J23100
sequence: https://synbiohub.org/public/igem/BBa_K1520003_sequence/1

BBa_J23100
type: Component
persistent identity: component2384884
display id: component2384884
version: 1
top level: BBa_K1520003
access: public
definition: https://synbiohub.org/public/igem/BBa_J23100/1

BBa_K1520501
type: Component
persistent identity: component2384887
display id: component2384887
version: 1
top level: BBa_K1520003
access: public
definition: BBa_K1520501

BBa_K1520029
type: Component
persistent identity: component2384888
display id: component2384888
version: 1
top level: BBa_K1520003
access: public
definition: https://synbiohub.org/public/igem/BBa_K1520029/1

BBa_K1520501
type: SequenceAnnotation
persistent identity: annotation2384887
display id: annotation2384887
version: 1
top level: BBa_K1520003
location: https://synbiohub.org/public/igem/BBa_K1520003/annotation2384887/range2384887/1
component: BBa_K1520501

https://synbiohub.org/public/igem/BBa_K1520003/annotation2384887/range2384887/1
type: Range
persistent identity: range2384887
display id: range2384887
version: 1
direction: direction/reverse
top level: BBa_K1520003
start: 44
end: 842
orientation: inline

BBa_K1520029
type: SequenceAnnotation
persistent identity: annotation2384888
display id: annotation2384888
version: 1
top level: BBa_K1520003
location: https://synbiohub.org/public/igem/BBa_K1520003/annotation2384888/range2384888/1
component: BBa_K1520029

https://synbiohub.org/public/igem/BBa_K1520003/annotation2384888/range2384888/1
type: Range
persistent identity: range2384888
display id: range2384888
version: 1
direction: direction/reverse
top level: BBa_K1520003
start: 851
end: 979
orientation: inline

BBa_J23100
type: SequenceAnnotation
persistent identity: annotation2384884
display id: annotation2384884
version: 1
top level: BBa_K1520003
location: https://synbiohub.org/public/igem/BBa_K1520003/annotation2384884/range2384884/1
component: BBa_J23100

https://synbiohub.org/public/igem/BBa_K1520003/annotation2384884/range2384884/1
type: Range
persistent identity: range2384884
display id: range2384884
version: 1
direction: direction/reverse
top level: BBa_K1520003
start: 1
end: 35
orientation: inline

BBa_K864200
type: ComponentDefinition
persistent identity: BBa K864200
display id: BBa_K864200
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: MarR native E. coli repressor
creator: Erik Lundin
created: 2012-09-24T11:00:00Z
modified: 2015-05-08T01:13:37Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1124_
m user id: 0
owner id: 9827
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: The marR gene encodes a repressor of the marRAB operon
mutable notes: None
mutable provenance: Amplified from MG1655 (Accession NC_000913) chromosome using primers listed below marR_BB_F TTCGAATTCGCGGCCGCTTCTAGATGAAAAGTACCAGCGATCT marR_BB_R GGCCGCTACTAGTATTATTACGGCAGGACTTTCTT
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: marR
sequence: https://synbiohub.org/public/igem/BBa_K864200_sequence/1

marR
type: SequenceAnnotation
persistent identity: annotation2197174
display id: annotation2197174
version: 1
top level: BBa_K864200
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K864200/annotation2197174/range2197174/1

https://synbiohub.org/public/igem/BBa_K864200/annotation2197174/range2197174/1
type: Range
persistent identity: range2197174
display id: range2197174
version: 1
direction: direction/forward
top level: BBa_K864200
start: 1
end: 438
orientation: inline

LacI
type: ComponentDefinition
persistent identity: BBa I732100
display id: BBa_I732100
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LacI
creator: Zhan Jian
created: 2007-10-13T11:00:00Z
modified: 2016-10-18T06:58:21Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _156_
m user id: 31248
owner id: 1557
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_I732100_sequence/1

LacIM
type: ComponentDefinition
persistent identity: BBa K819012
display id: BBa_K819012
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Luminesensor repressible LacIM generator
creator: ZHANG Hong
created: 2012-09-02T11:00:00Z
modified: 2015-05-08T01:13:29Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1077_
m user id: 0
owner id: 13785
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: LacI mutant driven by luminesensor repressible SulA promoter in 408 form.
mutable notes: B0034, CDS of LacI mutant and B0015 were connected directly to SulA promoter in 408 form.
mutable provenance: Sequene of the LacI mutant is from Partregistry.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: SulA408 Promoter
sequence annotation: LacIM(K395400)
sequence: https://synbiohub.org/public/igem/BBa_K819012_sequence/1

SulA408 Promoter
type: SequenceAnnotation
persistent identity: annotation2181628
display id: annotation2181628
version: 1
top level: LacIM
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K819012/annotation2181628/range2181628/1

https://synbiohub.org/public/igem/BBa_K819012/annotation2181628/range2181628/1
type: Range
persistent identity: range2181628
display id: range2181628
version: 1
direction: direction/forward
top level: LacIM
start: 1
end: 64
orientation: inline

LacIM(K395400)
type: SequenceAnnotation
persistent identity: annotation2181627
display id: annotation2181627
version: 1
top level: LacIM
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K819012/annotation2181627/range2181627/1

https://synbiohub.org/public/igem/BBa_K819012/annotation2181627/range2181627/1
type: Range
persistent identity: range2181627
display id: range2181627
version: 1
direction: direction/forward
top level: LacIM
start: 65
end: 1292
orientation: inline

lacI ts
type: ComponentDefinition
persistent identity: BBa J06501
display id: BBa_J06501
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LacI repressor (temperature-sensitive mut 265) (+LVA)
creator: ytwang
created: 2005-06-13T11:00:00Z
modified: 2015-08-31T04:08:18Z
discontinued: false
dominant: false
experience: experience/None
group u list: _20_
m user id: 0
owner id: 340
owning group id: 20
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Mutated from BBa_C0012 Repressor, LacI (LVA+) to be temperature sensitive. Mutation from Chao, et al., 2002: Gly265->Asp (GG(803)T->GAT)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: TS mutation (G->A)
sequence annotation: LVA
sequence annotation: barcode from LacI (BBa_C0012)
sequence annotation: LacI(ts)-LVA
sequence: https://synbiohub.org/public/igem/BBa_J06501_sequence/1

TS mutation (G->A)
type: SequenceAnnotation
persistent identity: annotation1530900
display id: annotation1530900
version: 1
top level: lacI ts
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_J06501/annotation1530900/range1530900/1

https://synbiohub.org/public/igem/BBa_J06501/annotation1530900/range1530900/1
type: Range
persistent identity: range1530900
display id: range1530900
version: 1
direction: direction/forward
top level: lacI ts
start: 803
end: 803
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation1530907
display id: annotation1530907
version: 1
top level: lacI ts
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_J06501/annotation1530907/range1530907/1

https://synbiohub.org/public/igem/BBa_J06501/annotation1530907/range1530907/1
type: Range
persistent identity: range1530907
display id: range1530907
version: 1
direction: direction/forward
top level: lacI ts
start: 1090
end: 1128
orientation: inline

barcode from LacI (BBa_C0012)
type: SequenceAnnotation
persistent identity: annotation1530914
display id: annotation1530914
version: 1
top level: lacI ts
role: SO:0000807
role: feature/barcode
location: https://synbiohub.org/public/igem/BBa_J06501/annotation1530914/range1530914/1

https://synbiohub.org/public/igem/BBa_J06501/annotation1530914/range1530914/1
type: Range
persistent identity: range1530914
display id: range1530914
version: 1
direction: direction/forward
top level: lacI ts
start: 1129
end: 1153
orientation: inline

LacI(ts)-LVA
type: SequenceAnnotation
persistent identity: annotation1530899
display id: annotation1530899
version: 1
top level: lacI ts
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J06501/annotation1530899/range1530899/1

https://synbiohub.org/public/igem/BBa_J06501/annotation1530899/range1530899/1
type: Range
persistent identity: range1530899
display id: range1530899
version: 1
direction: direction/forward
top level: lacI ts
start: 1
end: 1128
orientation: inline

BBa_K1520008
type: ComponentDefinition
persistent identity: BBa K1520008
display id: BBa_K1520008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Pcons2-rbs-lacI-Ter
creator: yue wu
created: 2014-09-21T11:00:00Z
modified: 2015-05-08T01:10:48Z
discontinued: false
dominant: false
group u list: _1902_
m user id: 0
owner id: 21318
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Pcons2-rbs-lacI-Ter. It constantly produces lacI.
mutable notes: None
mutable provenance: Pcons2, lacI, Ter
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_J23100
component: BBa_K1520502
component: BBa_K1520029
sequence annotation: BBa_K1520502
sequence annotation: BBa_J23100
sequence annotation: BBa_K1520029
sequence: https://synbiohub.org/public/igem/BBa_K1520008_sequence/1

BBa_J23100
type: Component
persistent identity: component2384932
display id: component2384932
version: 1
top level: BBa_K1520008
access: public
definition: https://synbiohub.org/public/igem/BBa_J23100/1

BBa_K1520502
type: Component
persistent identity: component2384935
display id: component2384935
version: 1
top level: BBa_K1520008
access: public
definition: BBa_K1520502

BBa_K1520029
type: Component
persistent identity: component2384936
display id: component2384936
version: 1
top level: BBa_K1520008
access: public
definition: https://synbiohub.org/public/igem/BBa_K1520029/1

BBa_K1520502
type: SequenceAnnotation
persistent identity: annotation2384935
display id: annotation2384935
version: 1
top level: BBa_K1520008
location: https://synbiohub.org/public/igem/BBa_K1520008/annotation2384935/range2384935/1
component: BBa_K1520502

https://synbiohub.org/public/igem/BBa_K1520008/annotation2384935/range2384935/1
type: Range
persistent identity: range2384935
display id: range2384935
version: 1
direction: direction/reverse
top level: BBa_K1520008
start: 44
end: 1214
orientation: inline

BBa_J23100
type: SequenceAnnotation
persistent identity: annotation2384932
display id: annotation2384932
version: 1
top level: BBa_K1520008
location: https://synbiohub.org/public/igem/BBa_K1520008/annotation2384932/range2384932/1
component: BBa_J23100

https://synbiohub.org/public/igem/BBa_K1520008/annotation2384932/range2384932/1
type: Range
persistent identity: range2384932
display id: range2384932
version: 1
direction: direction/reverse
top level: BBa_K1520008
start: 1
end: 35
orientation: inline

BBa_K1520029
type: SequenceAnnotation
persistent identity: annotation2384936
display id: annotation2384936
version: 1
top level: BBa_K1520008
location: https://synbiohub.org/public/igem/BBa_K1520008/annotation2384936/range2384936/1
component: BBa_K1520029

https://synbiohub.org/public/igem/BBa_K1520008/annotation2384936/range2384936/1
type: Range
persistent identity: range2384936
display id: range2384936
version: 1
direction: direction/reverse
top level: BBa_K1520008
start: 1223
end: 1351
orientation: inline

BBa_K142000
type: ComponentDefinition
persistent identity: BBa K142000
display id: BBa_K142000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: lacI IS mutant (IPTG unresponsive) R197A
creator: Julius Rabl
created: 2008-10-16T11:00:00Z
modified: 2015-05-08T01:10:21Z
discontinued: false
dominant: false
experience: experience/None
group u list: _194_
m user id: 0
owner id: 3254
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Short description: The lacI IS mutant is almost identical to the lacI transcriptional regulator except for the difference that it is not able to bind IPTG or allolactose due to a mutation; it therefore can not be activated by induction with these substances. Since it recognizes the same motif in the lac promotor region, it strongly represses transcription of all genes regulated by promotors with lacI binding site even if IPTG or allolactose are present. It can be used to terminate the expression of proteins under lac control if IPTG can not be removed from the cell rapidly. Detailed description: Expression of the lac operon in E. coli is tightly controlled by lacI, a protein, which binds to a repressor binding site within the promotor and disables transcription by obscuring the promotor region. When bound to DNA, lacI is in the tetrameric form, which consists of two dimers interacting at the end distal from the DNA binding site. Upon binding of allolactose or IPTG, the tetramer breaks down into two dimers and the affinity for the repressor binding site is greatly reduced; the lacI IPTG complex will diffuse away from the repressor binding site, leaving the promotor accessible. As a result of decades of genetic and structural studies, the function of lacI is now understood on the molecular level (1, 2). Mutational experiments have identified residues, which abolish IPTG response upon mutation (3). Furthermore, the x-ray crystal structure of lacI with bound IPTG has allowed the identification of residues that interact with IPTG and which are promising targets for mutagenesis (1). We decided to mutate residues R197 and T276, which are located in the IPTG binding groove, contact IPTG and have been shown to produce the lacI IS mutation in previous genetic experiments. Since a quantitative study of the strength of inhibition by different lacI IS mutants has to our knowledge not been published so far, we decided to generate a set of eight mutated lacIs, in which we replaced…
mutable notes: During ste-directed mutagenesis, the codons to be mutated were replaced with the most highly utilized codons in E. coli to prevent complications from the use of rare codons. The lacI IS sequences were analyzed for BioBrick restriction sites within the coding sequence to ensure their compatibility.
mutable provenance: Source: The lacI IS mutants presented derive from the BioBrick holding lacI by itself (C0012). Site-directed mutagenesis was performed by PCR, subsequent DpnI digest and transformation. The following primers were used: R197F forward CGGCGCGTCTGTTTCTGGCTGGCTG R197A forward CGGCGCGTCTGGCGCTGGCTGGCTG R197F reverse CAGCCAGCCAGAAACAGACGCGCCG R197A reverse CAGCCAGCCAGCGCCAGACGCGCCG T276F forward GGATACGACGATTTTGAAGACAGCTC T276A forward GGATACGACGATGCGGAAGACAGCTC T276F reverse GAGCTGTCTTCAAAATCGTCGTATCC T276A reverse GAGCTGTCTTCCGCATCGTCGTATCC All lacI IS holding BioBricks were verified by sequencing.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: lacI IS
sequence annotation: R197A
sequence: https://synbiohub.org/public/igem/BBa_K142000_sequence/1

lacI IS
type: SequenceAnnotation
persistent identity: annotation1981797
display id: annotation1981797
version: 1
top level: BBa_K142000
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K142000/annotation1981797/range1981797/1

https://synbiohub.org/public/igem/BBa_K142000/annotation1981797/range1981797/1
type: Range
persistent identity: range1981797
display id: range1981797
version: 1
direction: direction/forward
top level: BBa_K142000
start: 1
end: 1128
orientation: inline

R197A
type: SequenceAnnotation
persistent identity: annotation1981798
display id: annotation1981798
version: 1
top level: BBa_K142000
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K142000/annotation1981798/range1981798/1

https://synbiohub.org/public/igem/BBa_K142000/annotation1981798/range1981798/1
type: Range
persistent identity: range1981798
display id: range1981798
version: 1
direction: direction/forward
top level: BBa_K142000
start: 598
end: 600
orientation: inline

BBa_K142002
type: ComponentDefinition
persistent identity: BBa K142002
display id: BBa_K142002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: lacI IS mutant (IPTG unresponsive) T276A
creator: Julius Rabl
created: 2008-10-16T11:00:00Z
modified: 2015-05-08T01:10:22Z
discontinued: false
dominant: false
group u list: _194_
m user id: 0
owner id: 3254
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Short description: The lacI IS mutant is almost identical to the lacI transcriptional regulator except for the difference that it is not able to bind IPTG or allolactose due to a mutation; it therefore can not be activated by induction with these substances. Since it recognizes the same motif in the lac promotor region, it strongly represses transcription of all genes regulated by promotors with lacI binding site even if IPTG or allolactose are present. It can be used to terminate the expression of proteins under lac control if IPTG can not be removed from the cell rapidly. Detailed description: Expression of the lac operon in E. coli is tightly controlled by lacI, a protein, which binds to a repressor binding site within the promotor and disables transcription by obscuring the promotor region. When bound to DNA, lacI is in the tetrameric form, which consists of two dimers interacting at the end distal from the DNA binding site. Upon binding of allolactose or IPTG, the tetramer breaks down into two dimers and the affinity for the repressor binding site is greatly reduced; the lacI IPTG complex will diffuse away from the repressor binding site, leaving the promotor accessible. As a result of decades of genetic and structural studies, the function of lacI is now understood on the molecular level (1, 2). Mutational experiments have identified residues, which abolish IPTG response upon mutation (3). Furthermore, the x-ray crystal structure of lacI with bound IPTG has allowed the identification of residues that interact with IPTG and which are promising targets for mutagenesis (1). We decided to mutate residues R197 and T276, which are located in the IPTG binding groove, contact IPTG and have been shown to produce the lacI IS mutation in previous genetic experiments. Since a quantitative study of the strength of inhibition by different lacI IS mutants has to our knowledge not been published so far, we decided to generate a set of eight mutated lacIs, in which we replaced…
mutable notes: During site-directed mutagenesis, the codons to be mutated were replaced with the most highly utilized codons in E. coli to prevent complications from the use of rare codons. The lacI IS sequences were analyzed for BioBrick restriction sites within the coding sequence to ensure their compatibility.
mutable provenance: The lacI IS mutants presented derive from the BioBrick holding lacI by itself (C0012). Site-directed mutagenesis was performed by PCR, subsequent DpnI digest and transformation. The following primers were used: R197F forward CGGCGCGTCTGTTTCTGGCTGGCTG R197A forward CGGCGCGTCTGGCGCTGGCTGGCTG R197F reverse CAGCCAGCCAGAAACAGACGCGCCG R197A reverse CAGCCAGCCAGCGCCAGACGCGCCG T276F forward GGATACGACGATTTTGAAGACAGCTC T276A forward GGATACGACGATGCGGAAGACAGCTC T276F reverse GAGCTGTCTTCAAAATCGTCGTATCC T276A reverse GAGCTGTCTTCCGCATCGTCGTATCC All lacI IS holding BioBricks were verified by sequencing.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: T276A
sequence annotation: lacI IS
sequence: https://synbiohub.org/public/igem/BBa_K142002_sequence/1

T276A
type: SequenceAnnotation
persistent identity: annotation1981802
display id: annotation1981802
version: 1
top level: BBa_K142002
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K142002/annotation1981802/range1981802/1

https://synbiohub.org/public/igem/BBa_K142002/annotation1981802/range1981802/1
type: Range
persistent identity: range1981802
display id: range1981802
version: 1
direction: direction/forward
top level: BBa_K142002
start: 835
end: 837
orientation: inline

lacI IS
type: SequenceAnnotation
persistent identity: annotation1981801
display id: annotation1981801
version: 1
top level: BBa_K142002
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K142002/annotation1981801/range1981801/1

https://synbiohub.org/public/igem/BBa_K142002/annotation1981801/range1981801/1
type: Range
persistent identity: range1981801
display id: range1981801
version: 1
direction: direction/forward
top level: BBa_K142002
start: 1
end: 1128
orientation: inline

BBa_K1520519
type: ComponentDefinition
persistent identity: BBa K1520519
display id: BBa_K1520519
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PgolTS-golS-PgolB-tetR-Ter-PtetO-rfp-Ter
creator: yue wu
created: 2014-09-22T11:00:00Z
modified: 2015-05-08T01:10:49Z
discontinued: false
dominant: false
group u list: _1902_
m user id: 0
owner id: 21318
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: PgolTS-golS-PgolB-tetR-Ter-PtetO-rfp-Ter. If there exist gold ions, rfp will not be produced.
mutable notes: None
mutable provenance: PgolTS-golS-PgolB, tetR, PtetO, rfp, Ter
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1520014
component: BBa_K1520518
sequence annotation: BBa_K1520014
sequence annotation: BBa_K1520518
sequence: https://synbiohub.org/public/igem/BBa_K1520519_sequence/1

BBa_K1520014
type: Component
persistent identity: component2385760
display id: component2385760
version: 1
top level: BBa_K1520519
access: public
definition: https://synbiohub.org/public/igem/BBa_K1520014/1

BBa_K1520518
type: Component
persistent identity: component2385752
display id: component2385752
version: 1
top level: BBa_K1520519
access: public
definition: BBa_K1520518

BBa_K1520014
type: SequenceAnnotation
persistent identity: annotation2385760
display id: annotation2385760
version: 1
top level: BBa_K1520519
location: https://synbiohub.org/public/igem/BBa_K1520519/annotation2385760/range2385760/1
component: BBa_K1520014

https://synbiohub.org/public/igem/BBa_K1520519/annotation2385760/range2385760/1
type: Range
persistent identity: range2385760
display id: range2385760
version: 1
direction: direction/reverse
top level: BBa_K1520519
start: 1610
end: 2540
orientation: inline

BBa_K1520518
type: SequenceAnnotation
persistent identity: annotation2385752
display id: annotation2385752
version: 1
top level: BBa_K1520519
location: https://synbiohub.org/public/igem/BBa_K1520519/annotation2385752/range2385752/1
component: BBa_K1520518

https://synbiohub.org/public/igem/BBa_K1520519/annotation2385752/range2385752/1
type: Range
persistent identity: range2385752
display id: range2385752
version: 1
direction: direction/reverse
top level: BBa_K1520519
start: 1
end: 1601
orientation: inline

BBa_K142006
type: ComponentDefinition
persistent identity: BBa K142006
display id: BBa_K142006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: lacI IS mutant (IPTG unresponsive) R197F T276A
creator: Julius Rabl
created: 2008-10-24T11:00:00Z
modified: 2015-05-08T01:10:22Z
discontinued: false
dominant: false
experience: experience/None
group u list: _194_
m user id: 0
owner id: 3254
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: lacI IS mutant (IPTG unresponsive) Short description: The lacI IS mutant is almost identical to the lacI transcriptional regulator except for the difference that it is not able to bind IPTG or allolactose due to a mutation; it therefore can not be activated by induction with these substances. Since it recognizes the same motif in the lac promotor region, it strongly represses transcription of all genes regulated by promotors with lacI binding site even if IPTG or allolactose are present. It can be used to terminate the expression of proteins under lac control if IPTG can not be removed from the cell rapidly. Detailed description: Expression of the lac operon in E. coli is tightly controlled by lacI, a protein, which binds to a repressor binding site within the promotor and disables transcription by obscuring the promotor region. When bound to DNA, lacI is in the tetrameric form, which consists of two dimers interacting at the end distal from the DNA binding site. Upon binding of allolactose or IPTG, the tetramer breaks down into two dimers and the affinity for the repressor binding site is greatly reduced; the lacI IPTG complex will diffuse away from the repressor binding site, leaving the promotor accessible. As a result of decades of genetic and structural studies, the function of lacI is now understood on the molecular level (1, 2). Mutational experiments have identified residues, which abolish IPTG response upon mutation (3). Furthermore, the x-ray crystal structure of lacI with bound IPTG has allowed the identification of residues that interact with IPTG and which are promising targets for mutagenesis (1). We decided to mutate residues R197 and T276, which are located in the IPTG binding groove, contact IPTG and have been shown to produce the lacI IS mutation in previous genetic experiments. Since a quantitative study of the strength of inhibition by different lacI IS mutants has to our knowledge not been published so far, we decided to generate a set of eight …
mutable notes: During site-directed mutagenesis, the codons to be mutated were replaced with the most highly utilized codons in E. coli to prevent complications from the use of rare codons. The lacI IS sequences were analyzed for BioBrick restriction sites within the coding sequence to ensure their compatibility.
mutable provenance: The lacI IS mutants presented derive from the BioBrick holding lacI by itself (C0012). Site-directed mutagenesis was performed by PCR, subsequent DpnI digest and transformation. The following primers were used: R197F forward CGGCGCGTCTGTTTCTGGCTGGCTG R197A forward CGGCGCGTCTGGCGCTGGCTGGCTG R197F reverse CAGCCAGCCAGAAACAGACGCGCCG R197A reverse CAGCCAGCCAGCGCCAGACGCGCCG T276F forward GGATACGACGATTTTGAAGACAGCTC T276A forward GGATACGACGATGCGGAAGACAGCTC T276F reverse GAGCTGTCTTCAAAATCGTCGTATCC T276A reverse GAGCTGTCTTCCGCATCGTCGTATCC All lacI IS holding BioBricks were verified by sequencing.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: lacI IS
sequence: https://synbiohub.org/public/igem/BBa_K142006_sequence/1

lacI IS
type: SequenceAnnotation
persistent identity: annotation1987411
display id: annotation1987411
version: 1
top level: BBa_K142006
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K142006/annotation1987411/range1987411/1

https://synbiohub.org/public/igem/BBa_K142006/annotation1987411/range1987411/1
type: Range
persistent identity: range1987411
display id: range1987411
version: 1
direction: direction/forward
top level: BBa_K142006
start: 1
end: 1128
orientation: inline

BBa_K1031744
type: ComponentDefinition
persistent identity: BBa K1031744
display id: BBa_K1031744
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: J23114-PaaX
creator: He Shuaixin
created: 2013-09-08T11:00:00Z
modified: 2015-05-08T01:08:48Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1338_
m user id: 0
owner id: 15843
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: 114-PaaX-TT
mutable notes: molecular cloning
mutable provenance: PCR genome E.coli
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: PaaX
sequence annotation: BBa_B0034
sequence annotation: BBa_J23114
sequence: https://synbiohub.org/public/igem/BBa_K1031744_sequence/1

PaaX
type: SequenceAnnotation
persistent identity: annotation2357496
display id: annotation2357496
version: 1
top level: BBa_K1031744
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1031744/annotation2357496/range2357496/1

https://synbiohub.org/public/igem/BBa_K1031744/annotation2357496/range2357496/1
type: Range
persistent identity: range2357496
display id: range2357496
version: 1
direction: direction/forward
top level: BBa_K1031744
start: 62
end: 1012
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2357495
display id: annotation2357495
version: 1
top level: BBa_K1031744
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1031744/annotation2357495/range2357495/1

https://synbiohub.org/public/igem/BBa_K1031744/annotation2357495/range2357495/1
type: Range
persistent identity: range2357495
display id: range2357495
version: 1
direction: direction/forward
top level: BBa_K1031744
start: 44
end: 55
orientation: inline

BBa_J23114
type: SequenceAnnotation
persistent identity: annotation2357494
display id: annotation2357494
version: 1
top level: BBa_K1031744
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1031744/annotation2357494/range2357494/1

https://synbiohub.org/public/igem/BBa_K1031744/annotation2357494/range2357494/1
type: Range
persistent identity: range2357494
display id: range2357494
version: 1
direction: direction/forward
top level: BBa_K1031744
start: 1
end: 35
orientation: inline

BBa_K082005
type: ComponentDefinition
persistent identity: BBa K082005
display id: BBa_K082005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LacI-Mutant
creator: Yanting Xue, Binjie Xu
created: 2008-10-27T12:00:00Z
modified: 2015-05-08T01:08:35Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _228_
m user id: 0
owner id: 1560
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: None
mutable notes: None
mutable provenance: None
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: BBa_K082005
sequence: https://synbiohub.org/public/igem/BBa_K082005_sequence/1

BBa_K082005
type: SequenceAnnotation
persistent identity: annotation1992781
display id: annotation1992781
version: 1
top level: BBa_K082005
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K082005/annotation1992781/range1992781/1

https://synbiohub.org/public/igem/BBa_K082005/annotation1992781/range1992781/1
type: Range
persistent identity: range1992781
display id: range1992781
version: 1
direction: direction/reverse
top level: BBa_K082005
start: 1
end: 1083
orientation: inline

BBa_K1509000
type: ComponentDefinition
persistent identity: BBa K1509000
display id: BBa_K1509000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Coding for the trans-acting regulator SmtB
creator: Tong Li
created: 2014-09-25T11:00:00Z
modified: 2015-07-23T03:26:04Z
discontinued: false
dominant: false
group u list: _1889_
m user id: 4206
owner id: 24032
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: In 1993, Huckle and co-workers reported the isolation and analysis of the smt locus from Synechococcus sp.. Gene smtB, one of the components of the locus, encodes a transcriptional repressor of gene smtA, with which is required for normal tolerance to Zn2+ and Cd2+. The product of smtB functions by binding to the smt operator-promoter(smtO-P) and dissociating in the presence of Zn2+ due to the classical helix-turn-helix motif similar to many DNA-binding proteins. It can also be genomically rearranged when induced by Cd2+, leading to a deletion within smtB because of a highly iterated palindrome (HIP1), a highly represented octanucleotides(5′-GCGATC-GC-3′) which traverse both borders of the excised element. These two mechanisms both cause the elevated expression of smtA.
mutable notes: According to the mechanisms of smt locus represented above, gene smtB should function with the accompany of smt operator-promoter. Previous cases selected smtA whose product functions by encoding MT-like to sequester metal ions, while our case intended to take advantage of the trigger lies in this locus. By imitating former researches, in which a promoter less lacZ gene was fused upstream of smtA, we respectively splice two selected reporter genes and sequence from smt operator-promoter to smtB, equivalent to replacing smtA with reporter genes, to visualize the effects of metal ions on the locus.
mutable provenance: Metallothioneins(MTs), discovered in horse kidney in 1957 by Margoshes and Vallee are identified as low-molecular weight and sulphydryl rich proteins which bind metal ions in metal-thiolate clusters and whose synthesis increases in response to elevated concentrations of certain metals. In 1988, Olafson and co-workers reported the amino acid sequence of a metal-associated protein isolated from cyanobacterium Synechococcus sp.. With the function of binding metal ions remaining, it only has less than 20% homology in amino acid sequence with known MTs in eukaryotes and thus we call it class II MT or on some occasions MT-like. Owing to the works of Robinson and Huckle, the corresponding locus of Synechococcus PCC7942, smt locus was isolated and analyzed. It includes smtA which encodes the class II MT and a divergently transcribed gene smtB which encodes a trans-acting, autoregulatory repressor that exerts metal ion-inducible negative control over smtA transcription. Between them lies a 100-bp operator-promoter region(smt O-P, BBa_K1509001), which is responsible for the divergent orientation of smtA and smtB.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: smtB
sequence: https://synbiohub.org/public/igem/BBa_K1509000_sequence/1

smtB
type: SequenceAnnotation
persistent identity: annotation2387081
display id: annotation2387081
version: 1
top level: BBa_K1509000
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1509000/annotation2387081/range2387081/1

https://synbiohub.org/public/igem/BBa_K1509000/annotation2387081/range2387081/1
type: Range
persistent identity: range2387081
display id: range2387081
version: 1
direction: direction/forward
top level: BBa_K1509000
start: 1
end: 301
orientation: inline

BBa_K1682011
type: ComponentDefinition
persistent identity: BBa K1682011
display id: BBa_K1682011
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: NsrR
creator: iGEM15_HKUST-Rice
created: 2015-09-01T11:00:00Z
modified: 2015-09-18T09:29:08Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2100_
m user id: 8758
owner id: 26069
owning group id: 9
status: status/Planning
bookmark: false
mutable description: NsrR protein is a nitrate sensitive transcriptional repressor. When there is nitrate, it will be oxidized to nitric oxide by nitrate oxidase, which in turns relieve the repression of NsrR on PyeaR promoter.
mutable notes: N/A
mutable provenance: Native in E. coli
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: nsrR
sequence: https://synbiohub.org/public/igem/BBa_K1682011_sequence/1

nsrR
type: SequenceAnnotation
persistent identity: annotation2475311
display id: annotation2475311
version: 1
top level: BBa_K1682011
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1682011/annotation2475311/range2475311/1

https://synbiohub.org/public/igem/BBa_K1682011/annotation2475311/range2475311/1
type: Range
persistent identity: range2475311
display id: range2475311
version: 1
direction: direction/forward
top level: BBa_K1682011
start: 1
end: 426
orientation: inline

BBa_K142001
type: ComponentDefinition
persistent identity: BBa K142001
display id: BBa_K142001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: lacI IS mutant (IPTG unresponsive) R197F
creator: Julius Rabl
created: 2008-10-16T11:00:00Z
modified: 2015-05-08T01:10:22Z
discontinued: false
dominant: false
group u list: _194_
m user id: 0
owner id: 3254
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Short description: The lacI IS mutant is almost identical to the lacI transcriptional regulator except for the difference that it is not able to bind IPTG or allolactose due to a mutation; it therefore can not be activated by induction with these substances. Since it recognizes the same motif in the lac promotor region, it strongly represses transcription of all genes regulated by promotors with lacI binding site even if IPTG or allolactose are present. It can be used to terminate the expression of proteins under lac control if IPTG can not be removed from the cell rapidly. Detailed description: Expression of the lac operon in E. coli is tightly controlled by lacI, a protein, which binds to a repressor binding site within the promotor and disables transcription by obscuring the promotor region. When bound to DNA, lacI is in the tetrameric form, which consists of two dimers interacting at the end distal from the DNA binding site. Upon binding of allolactose or IPTG, the tetramer breaks down into two dimers and the affinity for the repressor binding site is greatly reduced; the lacI IPTG complex will diffuse away from the repressor binding site, leaving the promotor accessible. As a result of decades of genetic and structural studies, the function of lacI is now understood on the molecular level (1, 2). Mutational experiments have identified residues, which abolish IPTG response upon mutation (3). Furthermore, the x-ray crystal structure of lacI with bound IPTG has allowed the identification of residues that interact with IPTG and which are promising targets for mutagenesis (1). We decided to mutate residues R197 and T276, which are located in the IPTG binding groove, contact IPTG and have been shown to produce the lacI IS mutation in previous genetic experiments. Since a quantitative study of the strength of inhibition by different lacI IS mutants has to our knowledge not been published so far, we decided to generate a set of eight mutated lacIs, in which we replaced…
mutable notes: During site-directed mutagenesis, the codons to be mutated were replaced with the most highly utilized codons in E. coli to prevent complications from the use of rare codons. The lacI IS sequences were analyzed for BioBrick restriction sites within the coding sequence to ensure their compatibility.
mutable provenance: The lacI IS mutants presented derive from the BioBrick holding lacI by itself (C0012). Site-directed mutagenesis was performed by PCR, subsequent DpnI digest and transformation. The following primers were used: R197F forward CGGCGCGTCTGTTTCTGGCTGGCTG R197A forward CGGCGCGTCTGGCGCTGGCTGGCTG R197F reverse CAGCCAGCCAGAAACAGACGCGCCG R197A reverse CAGCCAGCCAGCGCCAGACGCGCCG T276F forward GGATACGACGATTTTGAAGACAGCTC T276A forward GGATACGACGATGCGGAAGACAGCTC T276F reverse GAGCTGTCTTCAAAATCGTCGTATCC T276A reverse GAGCTGTCTTCCGCATCGTCGTATCC All lacI IS holding BioBricks were verified by sequencing.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: lacI IS
sequence annotation: R197F
sequence: https://synbiohub.org/public/igem/BBa_K142001_sequence/1

lacI IS
type: SequenceAnnotation
persistent identity: annotation1981799
display id: annotation1981799
version: 1
top level: BBa_K142001
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K142001/annotation1981799/range1981799/1

https://synbiohub.org/public/igem/BBa_K142001/annotation1981799/range1981799/1
type: Range
persistent identity: range1981799
display id: range1981799
version: 1
direction: direction/forward
top level: BBa_K142001
start: 1
end: 1128
orientation: inline

R197F
type: SequenceAnnotation
persistent identity: annotation1981800
display id: annotation1981800
version: 1
top level: BBa_K142001
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K142001/annotation1981800/range1981800/1

https://synbiohub.org/public/igem/BBa_K142001/annotation1981800/range1981800/1
type: Range
persistent identity: range1981800
display id: range1981800
version: 1
direction: direction/forward
top level: BBa_K142001
start: 598
end: 600
orientation: inline

BBa_K1520007
type: ComponentDefinition
persistent identity: BBa K1520007
display id: BBa_K1520007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PcI-rbs-lacI-Ter
creator: yue wu
created: 2014-09-21T11:00:00Z
modified: 2015-05-08T01:10:48Z
discontinued: false
dominant: false
group u list: _1902_
m user id: 0
owner id: 21318
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: PcI-rbs-lacI-Ter. It produces enough lacI when there is less cI.
mutable notes: None
mutable provenance: PcI, lacI, Ter
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_R0051
component: BBa_K1520502
component: BBa_K1520029
sequence annotation: BBa_K1520029
sequence annotation: BBa_R0051
sequence annotation: BBa_K1520502
sequence: https://synbiohub.org/public/igem/BBa_K1520007_sequence/1

BBa_R0051
type: Component
persistent identity: component2384924
display id: component2384924
version: 1
top level: BBa_K1520007
access: public
definition: https://synbiohub.org/public/igem/BBa_R0051/1

BBa_K1520502
type: Component
persistent identity: component2384930
display id: component2384930
version: 1
top level: BBa_K1520007
access: public
definition: BBa_K1520502

BBa_K1520029
type: Component
persistent identity: component2384931
display id: component2384931
version: 1
top level: BBa_K1520007
access: public
definition: https://synbiohub.org/public/igem/BBa_K1520029/1

BBa_K1520029
type: SequenceAnnotation
persistent identity: annotation2384931
display id: annotation2384931
version: 1
top level: BBa_K1520007
location: https://synbiohub.org/public/igem/BBa_K1520007/annotation2384931/range2384931/1
component: BBa_K1520029

https://synbiohub.org/public/igem/BBa_K1520007/annotation2384931/range2384931/1
type: Range
persistent identity: range2384931
display id: range2384931
version: 1
direction: direction/reverse
top level: BBa_K1520007
start: 1237
end: 1365
orientation: inline

BBa_R0051
type: SequenceAnnotation
persistent identity: annotation2384924
display id: annotation2384924
version: 1
top level: BBa_K1520007
location: https://synbiohub.org/public/igem/BBa_K1520007/annotation2384924/range2384924/1
component: BBa_R0051

https://synbiohub.org/public/igem/BBa_K1520007/annotation2384924/range2384924/1
type: Range
persistent identity: range2384924
display id: range2384924
version: 1
direction: direction/forward
top level: BBa_K1520007
start: 1
end: 49
orientation: inline

BBa_K1520502
type: SequenceAnnotation
persistent identity: annotation2384930
display id: annotation2384930
version: 1
top level: BBa_K1520007
location: https://synbiohub.org/public/igem/BBa_K1520007/annotation2384930/range2384930/1
component: BBa_K1520502

https://synbiohub.org/public/igem/BBa_K1520007/annotation2384930/range2384930/1
type: Range
persistent identity: range2384930
display id: range2384930
version: 1
direction: direction/reverse
top level: BBa_K1520007
start: 58
end: 1228
orientation: inline

cI lam
type: ComponentDefinition
persistent identity: BBa C0051
display id: BBa_C0051
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: cI repressor from E. coli phage lambda (+LVA)
creator: Vinay S Mahajan, Brian Chow, Peter Carr, Voichita Marinescu and Alexander D. Wissner-Gross
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:23Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Coding region for the cI repressor based on cI repressor from bacteriophage lambda modified with an LVA tail for rapid degradation of the protein. cI repressor binds to the cI regulator (BBa_R0051).
mutable notes: References (unparsed) here: A synthetic oscillatory network of transcriptional regulators , Elowitz M.B. , Leibler S., Nature(403),335-38: 2000 Octamerization of CI repressor is needed for effective repression of PRM and efficient switching from lysogeny. Ian B. Dodd,1 Alison J. Perkins, Daniel Tsemitsidis, and J. Barry Egan , Genes and Development (Vol 15, No. 22) 3013-3022: 2001 References (unparsed) here: A synthetic oscillatory network of transcriptional regulators , Elowitz M.B. , Leibler S., Nature(403),335-38: 2000 Octamerization of CI repressor is needed for effective repression of PRM and efficient switching from lysogeny. Ian B. Dodd,1 Alison J. Perkins, Daniel Tsemitsidis, and J. Barry Egan , Genes and Development (Vol 15, No. 22) 3013-3022: 2001 BBa_C0051 cI repressor is based on the cI repressor from the Elowitz's repressilator. It has been modified to include a rapid degradation LAA tail, and includes the BioBrick standard assembly head and tail restriction sites. The RBS has been removed. The stop codon has been changed from TAA to a double stop codon TAATAA.
mutable provenance: Elowitz, M. B. Transport, Assembly, and Dynamics in Systems of Interacting Proteins. Thesis, Princeton Univ., Princeton (1999).
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: cI lambda
sequence annotation: Help:Barcodes
sequence annotation: LVA
sequence: https://synbiohub.org/public/igem/BBa_C0051_sequence/1

cI lambda
type: SequenceAnnotation
persistent identity: annotation23334
display id: annotation23334
version: 1
top level: cI lam
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_C0051/annotation23334/range23334/1

https://synbiohub.org/public/igem/BBa_C0051/annotation23334/range23334/1
type: Range
persistent identity: range23334
display id: range23334
version: 1
direction: direction/forward
top level: cI lam
start: 4
end: 711
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213991
display id: annotation2213991
version: 1
top level: cI lam
role: SO:0000807
role: feature/barcode
location: https://synbiohub.org/public/igem/BBa_C0051/annotation2213991/range2213991/1

https://synbiohub.org/public/igem/BBa_C0051/annotation2213991/range2213991/1
type: Range
persistent identity: range2213991
display id: range2213991
version: 1
direction: direction/forward
top level: cI lam
start: 751
end: 775
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation23335
display id: annotation23335
version: 1
top level: cI lam
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_C0051/annotation23335/range23335/1

https://synbiohub.org/public/igem/BBa_C0051/annotation23335/range23335/1
type: Range
persistent identity: range23335
display id: range23335
version: 1
direction: direction/forward
top level: cI lam
start: 712
end: 744
orientation: inline

BBa_K1480003
type: ComponentDefinition
persistent identity: BBa K1480003
display id: BBa_K1480003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TetR generator (Promoter J23109)
creator: Jesus Gilberto Rodriguez Ceja
created: 2014-10-13T11:00:00Z
modified: 2015-05-08T01:10:40Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1860_
m user id: 0
owner id: 12878
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is a TetR protein generator regulated by the constitutive promoter J23109. The protein has a LVA tag for degradation.
mutable notes: It was assembled using assembly 10.
mutable provenance: Based on parts of the registry.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0012
component: BBa_C0040
component: BBa_J23109
component: BBa_B0034
component: BBa_B0010
sequence annotation: BBa_C0040
sequence annotation: BBa_B0012
sequence annotation: BBa_B0010
sequence annotation: BBa_B0034
sequence annotation: BBa_J23109
sequence: https://synbiohub.org/public/igem/BBa_K1480003_sequence/1

BBa_B0012
type: Component
persistent identity: component2419537
display id: component2419537
version: 1
top level: BBa_K1480003
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_C0040
type: Component
persistent identity: component2419534
display id: component2419534
version: 1
top level: BBa_K1480003
access: public
definition: tetR

BBa_J23109
type: Component
persistent identity: component2419528
display id: component2419528
version: 1
top level: BBa_K1480003
access: public
definition: https://synbiohub.org/public/igem/BBa_J23109/1

BBa_B0034
type: Component
persistent identity: component2419530
display id: component2419530
version: 1
top level: BBa_K1480003
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0010
type: Component
persistent identity: component2419535
display id: component2419535
version: 1
top level: BBa_K1480003
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_C0040
type: SequenceAnnotation
persistent identity: annotation2419534
display id: annotation2419534
version: 1
top level: BBa_K1480003
location: https://synbiohub.org/public/igem/BBa_K1480003/annotation2419534/range2419534/1
component: BBa_C0040

https://synbiohub.org/public/igem/BBa_K1480003/annotation2419534/range2419534/1
type: Range
persistent identity: range2419534
display id: range2419534
version: 1
direction: direction/reverse
top level: BBa_K1480003
start: 62
end: 746
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2419537
display id: annotation2419537
version: 1
top level: BBa_K1480003
location: https://synbiohub.org/public/igem/BBa_K1480003/annotation2419537/range2419537/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K1480003/annotation2419537/range2419537/1
type: Range
persistent identity: range2419537
display id: range2419537
version: 1
direction: direction/forward
top level: BBa_K1480003
start: 843
end: 883
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2419535
display id: annotation2419535
version: 1
top level: BBa_K1480003
location: https://synbiohub.org/public/igem/BBa_K1480003/annotation2419535/range2419535/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K1480003/annotation2419535/range2419535/1
type: Range
persistent identity: range2419535
display id: range2419535
version: 1
direction: direction/forward
top level: BBa_K1480003
start: 755
end: 834
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2419530
display id: annotation2419530
version: 1
top level: BBa_K1480003
location: https://synbiohub.org/public/igem/BBa_K1480003/annotation2419530/range2419530/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1480003/annotation2419530/range2419530/1
type: Range
persistent identity: range2419530
display id: range2419530
version: 1
direction: direction/reverse
top level: BBa_K1480003
start: 44
end: 55
orientation: inline

BBa_J23109
type: SequenceAnnotation
persistent identity: annotation2419528
display id: annotation2419528
version: 1
top level: BBa_K1480003
location: https://synbiohub.org/public/igem/BBa_K1480003/annotation2419528/range2419528/1
component: BBa_J23109

https://synbiohub.org/public/igem/BBa_K1480003/annotation2419528/range2419528/1
type: Range
persistent identity: range2419528
display id: range2419528
version: 1
direction: direction/reverse
top level: BBa_K1480003
start: 1
end: 35
orientation: inline

BBa_K142004
type: ComponentDefinition
persistent identity: BBa K142004
display id: BBa_K142004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: lacI IS mutant (IPTG unresponsive) R197A T276A
creator: Julius Rabl
created: 2008-10-24T11:00:00Z
modified: 2015-05-08T01:10:22Z
discontinued: false
dominant: false
group u list: _194_
m user id: 0
owner id: 3254
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Short description: The lacI IS mutant is almost identical to the lacI transcriptional regulator except for the difference that it is not able to bind IPTG or allolactose due to a mutation; it therefore can not be activated by induction with these substances. Since it recognizes the same motif in the lac promotor region, it strongly represses transcription of all genes regulated by promotors with lacI binding site even if IPTG or allolactose are present. It can be used to terminate the expression of proteins under lac control if IPTG can not be removed from the cell rapidly. Detailed description: Expression of the lac operon in E. coli is tightly controlled by lacI, a protein, which binds to a repressor binding site within the promotor and disables transcription by obscuring the promotor region. When bound to DNA, lacI is in the tetrameric form, which consists of two dimers interacting at the end distal from the DNA binding site. Upon binding of allolactose or IPTG, the tetramer breaks down into two dimers and the affinity for the repressor binding site is greatly reduced; the lacI IPTG complex will diffuse away from the repressor binding site, leaving the promotor accessible. As a result of decades of genetic and structural studies, the function of lacI is now understood on the molecular level (1, 2). Mutational experiments have identified residues, which abolish IPTG response upon mutation (3). Furthermore, the x-ray crystal structure of lacI with bound IPTG has allowed the identification of residues that interact with IPTG and which are promising targets for mutagenesis (1). We decided to mutate residues R197 and T276, which are located in the IPTG binding groove, contact IPTG and have been shown to produce the lacI IS mutation in previous genetic experiments. Since a quantitative study of the strength of inhibition by different lacI IS mutants has to our knowledge not been published so far, we decided to generate a set of eight mutated lacIs, in which we replaced…
mutable notes: During site-directed mutagenesis, the codons to be mutated were replaced with the most highly utilized codons in E. coli to prevent complications from the use of rare codons. The lacI IS sequences were analyzed for BioBrick restriction sites within the coding sequence to ensure their compatibility.
mutable provenance: The lacI IS mutants presented derive from the BioBrick holding lacI by itself (C0012). Site-directed mutagenesis was performed by PCR, subsequent DpnI digest and transformation. The following primers were used: R197F forward CGGCGCGTCTGTTTCTGGCTGGCTG R197A forward CGGCGCGTCTGGCGCTGGCTGGCTG R197F reverse CAGCCAGCCAGAAACAGACGCGCCG R197A reverse CAGCCAGCCAGCGCCAGACGCGCCG T276F forward GGATACGACGATTTTGAAGACAGCTC T276A forward GGATACGACGATGCGGAAGACAGCTC T276F reverse GAGCTGTCTTCAAAATCGTCGTATCC T276A reverse GAGCTGTCTTCCGCATCGTCGTATCC All lacI IS holding BioBricks were verified by sequencing.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: lacI IS
sequence: https://synbiohub.org/public/igem/BBa_K142004_sequence/1

lacI IS
type: SequenceAnnotation
persistent identity: annotation1987409
display id: annotation1987409
version: 1
top level: BBa_K142004
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K142004/annotation1987409/range1987409/1

https://synbiohub.org/public/igem/BBa_K142004/annotation1987409/range1987409/1
type: Range
persistent identity: range1987409
display id: range1987409
version: 1
direction: direction/forward
top level: BBa_K142004
start: 1
end: 1128
orientation: inline

BBa_K1720004
type: ComponentDefinition
persistent identity: BBa K1720004
display id: BBa_K1720004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Human phosphodiesterase 5A gene silencing device NO.2
creator: Ying Guan, Yuanbin Cui
created: 2015-08-26T11:00:00Z
modified: 2015-09-18T07:29:07Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2140_
m user id: 25248
owner id: 25248
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This device is uesd for silencing the human phosphodiesterase 5A (PDE5A) gene.A U6 promoter driving a designed, synthetic shRNA-like miRNA followed by the terminator. PDE5A is a cGMP-binding, cGMP-specific phosphodiesterase, a member of the cyclic nucleotide phosphodiesterase family. This phosphodiesterase specifically hydrolyzes cGMP to 5'-GMP. It is involved in the regulation of intracellular concentrations of cyclic nucleotides and is important for smooth muscle relaxation in the cardiovascular system. We designed 3 silencing device and test their function at the same time.Here is the another two device :BBa_K1720003,BBaK172005 This device with a GFP reporter was then transfected into HEK293 cells by lentiviral vector.Once we silence the PDE5A gene the level of cGMP will be up regulated as a result. The positive control was HEK293 cells that treat with Sodium Nitroprusside ,a NO donator that activate sGC and up regulate the level of cGMP. A negative control was made by transfecting an empty vector that does not contain sGC subunit. We used Elisa to detect cGMP level. The results are as follow:
mutable notes: 1. Kukreja RC, Salloum FN, Das A: Cyclic Guanosine Monophosphate Signaling and Phosphodiesterase-5 Inhibitors in Cardioprotection. Journal Of the American College Of Cardiology 2012, 59(22):1921-1927. 2. Li L, Haider HK, Wang L, Lu G, Ashraf M: Adenoviral short hairpin RNA therapy targeting phosphodiesterase 5a relieves cardiac remodeling and dysfunction following myocardial infarction. American Journal Of Physiology-Heart And Circulatory Physiology 2012, 302(10):H2112-H2121. 3. Unwalla HJ, Li HT, Bahner I, Li MJ, Kohn D, Rossi JJ: Novel Pol II fusion promoter directs human immunodeficiency virus type 1-inducible coexpression of a short hairpin RNA and protein. Journal of virology 2006, 80(4):1863-1873.
mutable provenance: We designed the shRNA according to the homology segment sequence of PDE5A from NCBI
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
sequence: https://synbiohub.org/public/igem/BBa_K1720004_sequence/1

BBa_K1520023
type: ComponentDefinition
persistent identity: BBa K1520023
display id: BBa_K1520023
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Pcons2-rbs-lacI-Ter-Plac-rbs-luxI-Ter-Pcons2-rbs-luxR-Ter-Prlux-rbs-rfp-rbs-luxR-Ter
creator: yue wu
created: 2014-09-21T11:00:00Z
modified: 2015-05-08T01:10:49Z
discontinued: false
dominant: false
group u list: _1902_
m user id: 0
owner id: 21318
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Plasmid in positive feedback verification: Pcons2-rbs-lacI-Ter-Plac-rbs-luxI-Ter-Pcons2-rbs-luxR-Ter-Prlux-rbs-rfp-rbs-luxR-Ter. It is a positive feedback plasmid using luxR as key to feedback. Introducing IPTG will produce rfp and luxR, and luxR will work back to enhance production of rfp.
mutable notes: None
mutable provenance: Pcons2, lacI, Plac, luxI, luxR, Prlux, rfp, Ter
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1520020
component: BBa_K1520012
sequence annotation: BBa_K1520012
sequence annotation: BBa_K1520020
sequence: https://synbiohub.org/public/igem/BBa_K1520023_sequence/1

BBa_K1520020
type: Component
persistent identity: component2385213
display id: component2385213
version: 1
top level: BBa_K1520023
access: public
definition: BBa_K1520020

BBa_K1520012
type: Component
persistent identity: component2385224
display id: component2385224
version: 1
top level: BBa_K1520023
access: public
definition: BBa_K1520012

BBa_K1520012
type: SequenceAnnotation
persistent identity: annotation2385224
display id: annotation2385224
version: 1
top level: BBa_K1520023
location: https://synbiohub.org/public/igem/BBa_K1520023/annotation2385224/range2385224/1
component: BBa_K1520012

https://synbiohub.org/public/igem/BBa_K1520023/annotation2385224/range2385224/1
type: Range
persistent identity: range2385224
display id: range2385224
version: 1
direction: direction/reverse
top level: BBa_K1520023
start: 3361
end: 5099
orientation: inline

BBa_K1520020
type: SequenceAnnotation
persistent identity: annotation2385213
display id: annotation2385213
version: 1
top level: BBa_K1520023
location: https://synbiohub.org/public/igem/BBa_K1520023/annotation2385213/range2385213/1
component: BBa_K1520020

https://synbiohub.org/public/igem/BBa_K1520023/annotation2385213/range2385213/1
type: Range
persistent identity: range2385213
display id: range2385213
version: 1
direction: direction/reverse
top level: BBa_K1520023
start: 1
end: 3352
orientation: inline

ARL4A0604
type: ComponentDefinition
persistent identity: BBa I732115
display id: BBa_I732115
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: ARL4A0604
creator: Zhan Jian
created: 2007-10-13T11:00:00Z
modified: 2015-08-31T04:07:57Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_I732115_sequence/1

BBa_K108021
type: ComponentDefinition
persistent identity: BBa K108021
display id: BBa_K108021
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Fis
creator: Qi Liu
created: 2008-10-22T11:00:00Z
modified: 2015-05-08T01:09:04Z
discontinued: false
dominant: false
experience: experience/None
group u list: _222_
m user id: 0
owner id: 2671
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: global DNA-binding transcriptional dual regulator
mutable notes: PCR using primers with biobrick prefix and surfix, and then cloned into pSB1AC3.
mutable provenance: E.coli K12 DH10B genome
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Fis
sequence: https://synbiohub.org/public/igem/BBa_K108021_sequence/1

Fis
type: SequenceAnnotation
persistent identity: annotation1986976
display id: annotation1986976
version: 1
top level: BBa_K108021
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K108021/annotation1986976/range1986976/1

https://synbiohub.org/public/igem/BBa_K108021/annotation1986976/range1986976/1
type: Range
persistent identity: range1986976
display id: range1986976
version: 1
direction: direction/forward
top level: BBa_K108021
start: 1
end: 294
orientation: inline

luxr
type: ComponentDefinition
persistent identity: BBa C0062
display id: BBa_C0062
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: luxR repressor/activator, (no LVA?)
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:23Z
discontinued: false
dominant: true
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: In complex with HSL, LuxR binds to the Lux promoter, activating transcription from Pr <bb_part>BBa_R0062</bb_part>, and repressing transcription from Pl <bb_part>BBa_R0063</bb_part>. The lux cassette of V. fischeri contains a left and a right promoter. The right promoter gives weak constitutive expression of downstream genes.This expression is up-regulated by the action of the Lux activator, LuxR complexed to HSL. Two molecules of LuxR protein form a complex with two molecules the signalling compound homoserine lactone (HSL). This complex binds to a palindromic site on the promoter, increasing the rate of transcription.
mutable notes: 2 silent point mutants were introduced in the coding sequence to remove internal XbaI and PstI sites. Mutation sites were chosen to replace codons commonly used in E. coli with codons used at a similar frequency.
mutable provenance: V. fischeri <genbank>AF170104</genbank>
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Help:Barcodes
sequence annotation: prefix
sequence annotation: A
sequence annotation: T
sequence annotation: BBa_C0062
sequence annotation: luxR
sequence: https://synbiohub.org/public/igem/BBa_C0062_sequence/1

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213986
display id: annotation2213986
version: 1
top level: luxr
role: SO:0000807
role: feature/barcode
location: https://synbiohub.org/public/igem/BBa_C0062/annotation2213986/range2213986/1

https://synbiohub.org/public/igem/BBa_C0062/annotation2213986/range2213986/1
type: Range
persistent identity: range2213986
display id: range2213986
version: 1
direction: direction/forward
top level: luxr
start: 757
end: 781
orientation: inline

prefix
type: SequenceAnnotation
persistent identity: annotation1762
display id: annotation1762
version: 1
top level: luxr
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_C0062/annotation1762/range1762/1

https://synbiohub.org/public/igem/BBa_C0062/annotation1762/range1762/1
type: Range
persistent identity: range1762
display id: range1762
version: 1
direction: direction/forward
top level: luxr
start: 1
end: 2
orientation: inline

A
type: SequenceAnnotation
persistent identity: annotation1765
display id: annotation1765
version: 1
top level: luxr
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_C0062/annotation1765/range1765/1

https://synbiohub.org/public/igem/BBa_C0062/annotation1765/range1765/1
type: Range
persistent identity: range1765
display id: range1765
version: 1
direction: direction/forward
top level: luxr
start: 492
end: 492
orientation: inline

T
type: SequenceAnnotation
persistent identity: annotation1764
display id: annotation1764
version: 1
top level: luxr
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_C0062/annotation1764/range1764/1

https://synbiohub.org/public/igem/BBa_C0062/annotation1764/range1764/1
type: Range
persistent identity: range1764
display id: range1764
version: 1
direction: direction/forward
top level: luxr
start: 174
end: 174
orientation: inline

BBa_C0062
type: SequenceAnnotation
persistent identity: annotation7039
display id: annotation7039
version: 1
top level: luxr
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_C0062/annotation7039/range7039/1

https://synbiohub.org/public/igem/BBa_C0062/annotation7039/range7039/1
type: Range
persistent identity: range7039
display id: range7039
version: 1
direction: direction/forward
top level: luxr
start: 1
end: 756
orientation: inline

luxR
type: SequenceAnnotation
persistent identity: annotation1766
display id: annotation1766
version: 1
top level: luxr
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_C0062/annotation1766/range1766/1

https://synbiohub.org/public/igem/BBa_C0062/annotation1766/range1766/1
type: Range
persistent identity: range1766
display id: range1766
version: 1
direction: direction/forward
top level: luxr
start: 1
end: 750
orientation: inline

LacI_I12
type: ComponentDefinition
persistent identity: BBa K091122
display id: BBa_K091122
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LacI_I12 protein
creator: Pallavi Penumetcha
created: 2008-07-08T11:00:00Z
modified: 2015-05-08T01:08:38Z
discontinued: false
dominant: false
experience: experience/None
group u list: _191_
m user id: 0
owner id: 2993
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This is a variation of the LacI protein that acts as a repressor for the pLac promoter and is repressed by IPTG. It has a point mutation at the third codon where proline is replaced by tyrosine. This missense mutation causes an increased binding affinity of the LacI_I12 protein with the pLac promoter.
mutable notes: Because there are many codons that code for the amino acid tyrosine, we chose to use the TAT codon, because it was the most common tyrosine codon found throughout the rest of the LacI wild-type gene sequence.
mutable provenance: The basic sequence comes from Ecogene.com
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Proline->Tyrosine
sequence: https://synbiohub.org/public/igem/BBa_K091122_sequence/1

Proline->Tyrosine
type: SequenceAnnotation
persistent identity: annotation1965746
display id: annotation1965746
version: 1
top level: LacI_I12
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K091122/annotation1965746/range1965746/1

https://synbiohub.org/public/igem/BBa_K091122/annotation1965746/range1965746/1
type: Range
persistent identity: range1965746
display id: range1965746
version: 1
direction: direction/reverse
top level: LacI_I12
start: 7
end: 9
orientation: inline

BBa_K1520513
type: ComponentDefinition
persistent identity: BBa K1520513
display id: BBa_K1520513
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: MC7-rbs-tetR-Ter-PtetO-rbs-rfp-Ter-Plac-rbs-tetR-Ter-Pcons2-rbs-lacI-Ter
creator: yue wu
created: 2014-09-22T11:00:00Z
modified: 2015-05-08T01:10:49Z
discontinued: false
dominant: false
group u list: _1902_
m user id: 0
owner id: 21318
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: MC7-rbs-tetR-Ter-PtetO-rbs-rfp-Ter-Plac-rbs-tetR-Ter-Pcons2-rbs-lacI-Ter It is a test of our suicide circuit for microcystin.
mutable notes: None
mutable provenance: MC17, tetR, PtetO, rfp, Plac, Pcons2, lacI, Ter
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1520029
component: BBa_K1520001
component: BBa_K1520505
component: BBa_K1520026
sequence annotation: BBa_K1520001
sequence annotation: BBa_K1520505
sequence annotation: BBa_K1520026
sequence annotation: BBa_K1520029
sequence: https://synbiohub.org/public/igem/BBa_K1520513_sequence/1

BBa_K1520029
type: Component
persistent identity: component2385604
display id: component2385604
version: 1
top level: BBa_K1520513
access: public
definition: https://synbiohub.org/public/igem/BBa_K1520029/1

BBa_K1520001
type: Component
persistent identity: component2385600
display id: component2385600
version: 1
top level: BBa_K1520513
access: public
definition: https://synbiohub.org/public/igem/BBa_K1520001/1

BBa_K1520505
type: Component
persistent identity: component2385603
display id: component2385603
version: 1
top level: BBa_K1520513
access: public
definition: BBa_K1520505

BBa_K1520026
type: Component
persistent identity: component2385632
display id: component2385632
version: 1
top level: BBa_K1520513
access: public
definition: https://synbiohub.org/public/igem/BBa_K1520026/1

BBa_K1520001
type: SequenceAnnotation
persistent identity: annotation2385600
display id: annotation2385600
version: 1
top level: BBa_K1520513
location: https://synbiohub.org/public/igem/BBa_K1520513/annotation2385600/range2385600/1
component: BBa_K1520001

https://synbiohub.org/public/igem/BBa_K1520513/annotation2385600/range2385600/1
type: Range
persistent identity: range2385600
display id: range2385600
version: 1
direction: direction/reverse
top level: BBa_K1520513
start: 1
end: 133
orientation: inline

BBa_K1520505
type: SequenceAnnotation
persistent identity: annotation2385603
display id: annotation2385603
version: 1
top level: BBa_K1520513
location: https://synbiohub.org/public/igem/BBa_K1520513/annotation2385603/range2385603/1
component: BBa_K1520505

https://synbiohub.org/public/igem/BBa_K1520513/annotation2385603/range2385603/1
type: Range
persistent identity: range2385603
display id: range2385603
version: 1
direction: direction/reverse
top level: BBa_K1520513
start: 142
end: 844
orientation: inline

BBa_K1520026
type: SequenceAnnotation
persistent identity: annotation2385632
display id: annotation2385632
version: 1
top level: BBa_K1520513
location: https://synbiohub.org/public/igem/BBa_K1520513/annotation2385632/range2385632/1
component: BBa_K1520026

https://synbiohub.org/public/igem/BBa_K1520513/annotation2385632/range2385632/1
type: Range
persistent identity: range2385632
display id: range2385632
version: 1
direction: direction/reverse
top level: BBa_K1520513
start: 990
end: 4335
orientation: inline

BBa_K1520029
type: SequenceAnnotation
persistent identity: annotation2385604
display id: annotation2385604
version: 1
top level: BBa_K1520513
location: https://synbiohub.org/public/igem/BBa_K1520513/annotation2385604/range2385604/1
component: BBa_K1520029

https://synbiohub.org/public/igem/BBa_K1520513/annotation2385604/range2385604/1
type: Range
persistent identity: range2385604
display id: range2385604
version: 1
direction: direction/reverse
top level: BBa_K1520513
start: 853
end: 981
orientation: inline

BBa_K864203
type: ComponentDefinition
persistent identity: BBa K864203
display id: BBa_K864203
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LexA3 super-repressor
creator: Erik Lundin
created: 2012-09-24T11:00:00Z
modified: 2015-05-08T01:13:37Z
discontinued: false
dominant: false
group u list: _1124_
m user id: 0
owner id: 9827
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: lexA3
mutable notes: lexA3 is lexA (BBa_K864201) with a G85D mutation (G>A basepair change at position 254)
mutable provenance: BBa_K864201
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: G95D
sequence annotation: lexA3
sequence annotation: G>A
sequence: https://synbiohub.org/public/igem/BBa_K864203_sequence/1

G95D
type: SequenceAnnotation
persistent identity: annotation2197172
display id: annotation2197172
version: 1
top level: BBa_K864203
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K864203/annotation2197172/range2197172/1

https://synbiohub.org/public/igem/BBa_K864203/annotation2197172/range2197172/1
type: Range
persistent identity: range2197172
display id: range2197172
version: 1
direction: direction/forward
top level: BBa_K864203
start: 253
end: 255
orientation: inline

lexA3
type: SequenceAnnotation
persistent identity: annotation2197171
display id: annotation2197171
version: 1
top level: BBa_K864203
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K864203/annotation2197171/range2197171/1

https://synbiohub.org/public/igem/BBa_K864203/annotation2197171/range2197171/1
type: Range
persistent identity: range2197171
display id: range2197171
version: 1
direction: direction/forward
top level: BBa_K864203
start: 1
end: 612
orientation: inline

G>A
type: SequenceAnnotation
persistent identity: annotation2197173
display id: annotation2197173
version: 1
top level: BBa_K864203
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K864203/annotation2197173/range2197173/1

https://synbiohub.org/public/igem/BBa_K864203/annotation2197173/range2197173/1
type: Range
persistent identity: range2197173
display id: range2197173
version: 1
direction: direction/forward
top level: BBa_K864203
start: 254
end: 254
orientation: inline

araC
type: ComponentDefinition
persistent identity: BBa C0080
display id: BBa_C0080
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: araC arabinose operon regulatory protein (repressor/activator) from E. coli (+LVA)
creator: Sara Neves (Fighting Darwins)
created: 2004-01-27T12:00:00Z
modified: 2015-08-31T04:07:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: coding region for the araC gene, used to make araC protein for use in positive or negative regulation of TIPs output (see also R0080, R0081)
mutable provenance: GenBank: NC_002655 (www.ncbi.nlm.nih.gov)
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Help:Barcodes
sequence annotation: araC
sequence annotation: LVA
sequence: https://synbiohub.org/public/igem/BBa_C0080_sequence/1

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2214004
display id: annotation2214004
version: 1
top level: araC
role: SO:0000807
role: feature/barcode
location: https://synbiohub.org/public/igem/BBa_C0080/annotation2214004/range2214004/1

https://synbiohub.org/public/igem/BBa_C0080/annotation2214004/range2214004/1
type: Range
persistent identity: range2214004
display id: range2214004
version: 1
direction: direction/forward
top level: araC
start: 916
end: 940
orientation: inline

araC
type: SequenceAnnotation
persistent identity: annotation308191
display id: annotation308191
version: 1
top level: araC
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_C0080/annotation308191/range308191/1

https://synbiohub.org/public/igem/BBa_C0080/annotation308191/range308191/1
type: Range
persistent identity: range308191
display id: range308191
version: 1
direction: direction/forward
top level: araC
start: 1
end: 876
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation308196
display id: annotation308196
version: 1
top level: araC
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_C0080/annotation308196/range308196/1

https://synbiohub.org/public/igem/BBa_C0080/annotation308196/range308196/1
type: Range
persistent identity: range308196
display id: range308196
version: 1
direction: direction/forward
top level: araC
start: 877
end: 909
orientation: inline

BBa_K1520020
type: ComponentDefinition
persistent identity: BBa K1520020
display id: BBa_K1520020
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Pcons2-rbs-lacI-Ter-Plac-rbs-luxI-Ter-Pcons2-rbs-luxR-Ter
creator: yue wu
created: 2014-09-21T11:00:00Z
modified: 2015-05-08T01:10:49Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1902_
m user id: 0
owner id: 21318
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Pcons2-rbs-lacI-Ter-Plac-rbs-luxI-Ter It produces luxI when there is enough IPTG.
mutable notes: None
mutable provenance: Pcons2, lacI, Plac, luxI, Ter
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1520019
component: BBa_K1520003
sequence annotation: BBa_K1520019
sequence annotation: BBa_K1520003
sequence: https://synbiohub.org/public/igem/BBa_K1520020_sequence/1

BBa_K1520019
type: Component
persistent identity: component2385110
display id: component2385110
version: 1
top level: BBa_K1520020
access: public
definition: https://synbiohub.org/public/igem/BBa_K1520019/1

BBa_K1520003
type: Component
persistent identity: component2385116
display id: component2385116
version: 1
top level: BBa_K1520020
access: public
definition: BBa_K1520003

BBa_K1520019
type: SequenceAnnotation
persistent identity: annotation2385110
display id: annotation2385110
version: 1
top level: BBa_K1520020
location: https://synbiohub.org/public/igem/BBa_K1520020/annotation2385110/range2385110/1
component: BBa_K1520019

https://synbiohub.org/public/igem/BBa_K1520020/annotation2385110/range2385110/1
type: Range
persistent identity: range2385110
display id: range2385110
version: 1
direction: direction/reverse
top level: BBa_K1520020
start: 1
end: 2365
orientation: inline

BBa_K1520003
type: SequenceAnnotation
persistent identity: annotation2385116
display id: annotation2385116
version: 1
top level: BBa_K1520020
location: https://synbiohub.org/public/igem/BBa_K1520020/annotation2385116/range2385116/1
component: BBa_K1520003

https://synbiohub.org/public/igem/BBa_K1520020/annotation2385116/range2385116/1
type: Range
persistent identity: range2385116
display id: range2385116
version: 1
direction: direction/reverse
top level: BBa_K1520020
start: 2374
end: 3352
orientation: inline

BBa_K1480004
type: ComponentDefinition
persistent identity: BBa K1480004
display id: BBa_K1480004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TetR generator (Promoter J23106 )
creator: Jesus Gilberto Rodriguez Ceja
created: 2014-10-13T11:00:00Z
modified: 2015-05-08T01:10:40Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1860_
m user id: 0
owner id: 12878
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is a TetR protein generator regulated by the constitutive promoter J23106. The protein has a LVA tag for degradation.
mutable notes: Based on parts of registry.
mutable provenance: It was assembled using assembly 10.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0034
component: BBa_C0040
component: BBa_B0012
component: BBa_B0010
component: BBa_J23106
sequence annotation: BBa_J23106
sequence annotation: BBa_B0010
sequence annotation: BBa_C0040
sequence annotation: BBa_B0034
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_K1480004_sequence/1

BBa_B0034
type: Component
persistent identity: component2419543
display id: component2419543
version: 1
top level: BBa_K1480004
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_C0040
type: Component
persistent identity: component2419547
display id: component2419547
version: 1
top level: BBa_K1480004
access: public
definition: tetR

BBa_B0012
type: Component
persistent identity: component2419550
display id: component2419550
version: 1
top level: BBa_K1480004
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0010
type: Component
persistent identity: component2419548
display id: component2419548
version: 1
top level: BBa_K1480004
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_J23106
type: Component
persistent identity: component2419541
display id: component2419541
version: 1
top level: BBa_K1480004
access: public
definition: https://synbiohub.org/public/igem/BBa_J23106/1

BBa_J23106
type: SequenceAnnotation
persistent identity: annotation2419541
display id: annotation2419541
version: 1
top level: BBa_K1480004
location: https://synbiohub.org/public/igem/BBa_K1480004/annotation2419541/range2419541/1
component: BBa_J23106

https://synbiohub.org/public/igem/BBa_K1480004/annotation2419541/range2419541/1
type: Range
persistent identity: range2419541
display id: range2419541
version: 1
direction: direction/reverse
top level: BBa_K1480004
start: 1
end: 35
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2419548
display id: annotation2419548
version: 1
top level: BBa_K1480004
location: https://synbiohub.org/public/igem/BBa_K1480004/annotation2419548/range2419548/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K1480004/annotation2419548/range2419548/1
type: Range
persistent identity: range2419548
display id: range2419548
version: 1
direction: direction/forward
top level: BBa_K1480004
start: 755
end: 834
orientation: inline

BBa_C0040
type: SequenceAnnotation
persistent identity: annotation2419547
display id: annotation2419547
version: 1
top level: BBa_K1480004
location: https://synbiohub.org/public/igem/BBa_K1480004/annotation2419547/range2419547/1
component: BBa_C0040

https://synbiohub.org/public/igem/BBa_K1480004/annotation2419547/range2419547/1
type: Range
persistent identity: range2419547
display id: range2419547
version: 1
direction: direction/reverse
top level: BBa_K1480004
start: 62
end: 746
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2419543
display id: annotation2419543
version: 1
top level: BBa_K1480004
location: https://synbiohub.org/public/igem/BBa_K1480004/annotation2419543/range2419543/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1480004/annotation2419543/range2419543/1
type: Range
persistent identity: range2419543
display id: range2419543
version: 1
direction: direction/reverse
top level: BBa_K1480004
start: 44
end: 55
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2419550
display id: annotation2419550
version: 1
top level: BBa_K1480004
location: https://synbiohub.org/public/igem/BBa_K1480004/annotation2419550/range2419550/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K1480004/annotation2419550/range2419550/1
type: Range
persistent identity: range2419550
display id: range2419550
version: 1
direction: direction/forward
top level: BBa_K1480004
start: 843
end: 883
orientation: inline

BBa_K142007
type: ComponentDefinition
persistent identity: BBa K142007
display id: BBa_K142007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: lacI IS mutant (IPTG unresponsive) R197F T276F
creator: Julius Rabl
created: 2008-10-24T11:00:00Z
modified: 2015-05-08T01:10:22Z
discontinued: false
dominant: false
group u list: _194_
m user id: 0
owner id: 3254
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: lacI IS mutant (IPTG unresponsive) Short description: The lacI IS mutant is almost identical to the lacI transcriptional regulator except for the difference that it is not able to bind IPTG or allolactose due to a mutation; it therefore can not be activated by induction with these substances. Since it recognizes the same motif in the lac promotor region, it strongly represses transcription of all genes regulated by promotors with lacI binding site even if IPTG or allolactose are present. It can be used to terminate the expression of proteins under lac control if IPTG can not be removed from the cell rapidly. Detailed description: Expression of the lac operon in E. coli is tightly controlled by lacI, a protein, which binds to a repressor binding site within the promotor and disables transcription by obscuring the promotor region. When bound to DNA, lacI is in the tetrameric form, which consists of two dimers interacting at the end distal from the DNA binding site. Upon binding of allolactose or IPTG, the tetramer breaks down into two dimers and the affinity for the repressor binding site is greatly reduced; the lacI IPTG complex will diffuse away from the repressor binding site, leaving the promotor accessible. As a result of decades of genetic and structural studies, the function of lacI is now understood on the molecular level (1, 2). Mutational experiments have identified residues, which abolish IPTG response upon mutation (3). Furthermore, the x-ray crystal structure of lacI with bound IPTG has allowed the identification of residues that interact with IPTG and which are promising targets for mutagenesis (1). We decided to mutate residues R197 and T276, which are located in the IPTG binding groove, contact IPTG and have been shown to produce the lacI IS mutation in previous genetic experiments. Since a quantitative study of the strength of inhibition by different lacI IS mutants has to our knowledge not been published so far, we decided to generate a set of eight …
mutable notes: During site-directed mutagenesis, the codons to be mutated were replaced with the most highly utilized codons in E. coli to prevent complications from the use of rare codons. The lacI IS sequences were analyzed for BioBrick restriction sites within the coding sequence to ensure their compatibility.
mutable provenance: The lacI IS mutants presented derive from the BioBrick holding lacI by itself (C0012). Site-directed mutagenesis was performed by PCR, subsequent DpnI digest and transformation. The following primers were used: R197F forward CGGCGCGTCTGTTTCTGGCTGGCTG R197A forward CGGCGCGTCTGGCGCTGGCTGGCTG R197F reverse CAGCCAGCCAGAAACAGACGCGCCG R197A reverse CAGCCAGCCAGCGCCAGACGCGCCG T276F forward GGATACGACGATTTTGAAGACAGCTC T276A forward GGATACGACGATGCGGAAGACAGCTC T276F reverse GAGCTGTCTTCAAAATCGTCGTATCC T276A reverse GAGCTGTCTTCCGCATCGTCGTATCC All lacI IS holding BioBricks were verified by sequencing.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: lacI IS
sequence: https://synbiohub.org/public/igem/BBa_K142007_sequence/1

lacI IS
type: SequenceAnnotation
persistent identity: annotation1987412
display id: annotation1987412
version: 1
top level: BBa_K142007
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K142007/annotation1987412/range1987412/1

https://synbiohub.org/public/igem/BBa_K142007/annotation1987412/range1987412/1
type: Range
persistent identity: range1987412
display id: range1987412
version: 1
direction: direction/forward
top level: BBa_K142007
start: 1
end: 1128
orientation: inline

BBa_K106668
type: ComponentDefinition
persistent identity: BBa K106668
display id: BBa_K106668
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Tet Repressor, AarI AB part
creator: Andrej Ondracka
created: 2008-10-24T11:00:00Z
modified: 2015-05-08T01:08:59Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _226_
m user id: 0
owner id: 3416
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: Tet repressor from E.coli
mutable notes: .
mutable provenance: .
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K106668_sequence/1

BBa_K091121
type: ComponentDefinition
persistent identity: BBa K091121
display id: BBa_K091121
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LacI wild-type gene
creator: Pallavi Penumetcha
created: 2008-07-08T11:00:00Z
modified: 2015-05-08T01:08:37Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _191_
m user id: 0
owner id: 2993
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: LacI wild-type gene sequence. Repressed by IPTG
mutable notes: We changed the start codon from CTG to ATG
mutable provenance: The gene sequence was obtained from Ecogene.com
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K091121_sequence/1

BBa_K950001
type: ComponentDefinition
persistent identity: BBa K950001
display id: BBa_K950001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: yeast rox1
creator: Jakob Matthes, Jan Rudolph
created: 2012-07-28T11:00:00Z
modified: 2015-05-08T01:13:48Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1218_
m user id: 0
owner id: 11576
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: rox1 is a repressor. It targets the promotor anb1.
mutable notes: original sequence was not altered
mutable provenance: Sequence and information is based on the Saccharomyces Genome Database, see http://www.yeastgenome.org/cgi-bin/locus.fpl?locus=rox1
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: rox1
sequence: https://synbiohub.org/public/igem/BBa_K950001_sequence/1

rox1
type: SequenceAnnotation
persistent identity: annotation2179266
display id: annotation2179266
version: 1
top level: BBa_K950001
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K950001/annotation2179266/range2179266/1

https://synbiohub.org/public/igem/BBa_K950001/annotation2179266/range2179266/1
type: Range
persistent identity: range2179266
display id: range2179266
version: 1
direction: direction/forward
top level: BBa_K950001
start: 1
end: 1107
orientation: inline

ARL2A0102
type: ComponentDefinition
persistent identity: BBa I732106
display id: BBa_I732106
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: ARL2A0102
creator: Zhan Jian
created: 2007-10-13T11:00:00Z
modified: 2015-08-31T04:07:57Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_I732106_sequence/1