//chassis/bacteriophage
type: Collection
persistent identity: chassis bacteriophage
display id: chassis_bacteriophage
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
member: BBa_K1031011
member: P3
member: //chassis/bacteriophage/t7
member: //chassis/bacteriophage/t4
member: //chassis/bacteriophage/t3

//chassis/bacteriophage/t4
type: Collection
persistent identity: chassis bacteriophage t4
display id: chassis_bacteriophage_t4
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
member: BBa_K112000

//chassis/bacteriophage/t3
type: Collection
persistent identity: chassis bacteriophage t3
display id: chassis_bacteriophage_t3
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
member: BBa_K2084000
member: BBa_K346000
member: BBa_K1833002
member: BBa_K1833997
member: BBa_K1479005
member: BBa_K2084001
member: BBa_K2084004

//chassis/bacteriophage/t7
type: Collection
persistent identity: chassis bacteriophage t7
display id: chassis_bacteriophage_t7
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
member: BBa_S03652
member: BBa_Z0253
member: BBa_K113011
member: BBa_K921001
member: BBa_K113010
member: BBa_J34814
member: BBa_R0186
member: BBa_R0182
member: T7
member: BBa_K113012
member: BBa_K1833000
member: BBa_K1833999
member: BBa_K921000
member: BBa_S03651
member: BBa_K1614000
member: BBa_R0187
member: BBa_Z0252
member: BBa_K1833009
member: BBa_R0183
member: BBa_J64997
member: BBa_I712074
member: BBa_S03650
member: pT7
member: BBa_Z0251
member: BBa_R0180
member: BBa_R0085
member: BBa_R0184
member: T7Gene6
member: BBa_K921002
member: BBa_S03653
member: BBa_R0181
member: BBa_K731700
member: BBa_R0185

BBa_B0030
type: ComponentDefinition
persistent identity: BBa B0030
display id: BBa_B0030
version: 1
was generated by: iGEM to SBOL conversion
description: RBS.1 (strong) -- modified from R. Weiss
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>.
discontinued: false
dominant: true
experience: experience/Works
group u list: _44_46_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("orig" in figure 4-14 of Ron Weiss thesis). No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: RBS
sequence annotation: BBa_B0030
sequence annotation: RBS-1\Strong
sequence: https://synbiohub.org/public/igem/BBa_B0030_sequence/1

RBS
type: SequenceAnnotation
persistent identity: annotation1702
display id: annotation1702
version: 1
top level: BBa_B0030
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_B0030/annotation1702/range1702/1

https://synbiohub.org/public/igem/BBa_B0030/annotation1702/range1702/1
type: Range
persistent identity: range1702
display id: range1702
version: 1
top level: BBa_B0030
direction: direction/forward
start: 8
end: 12
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation7025
display id: annotation7025
version: 1
top level: BBa_B0030
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_B0030/annotation7025/range7025/1

https://synbiohub.org/public/igem/BBa_B0030/annotation7025/range7025/1
type: Range
persistent identity: range7025
display id: range7025
version: 1
top level: BBa_B0030
direction: direction/forward
start: 1
end: 15
orientation: inline

RBS-1\Strong
type: SequenceAnnotation
persistent identity: annotation1701
display id: annotation1701
version: 1
top level: BBa_B0030
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_B0030/annotation1701/range1701/1

https://synbiohub.org/public/igem/BBa_B0030/annotation1701/range1701/1
type: Range
persistent identity: range1701
display id: range1701
version: 1
top level: BBa_B0030
direction: direction/forward
start: 1
end: 15
orientation: inline

BBa_K1031011
type: ComponentDefinition
persistent identity: BBa K1031011
display id: BBa_K1031011
version: 1
was generated by: iGEM to SBOL conversion
description: B0034-RiboJ-φR73
creator: He Shuaixin
created: 2013-09-08T11:00:00Z
modified: 2015-05-08T01:08:47Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1338_
m user id: 0
owner id: 15843
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: phiR-73
mutable notes: phiR73 phage
mutable provenance: lab made
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: phiR73
sequence: https://synbiohub.org/public/igem/BBa_K1031011_sequence/1

phiR73
type: SequenceAnnotation
persistent identity: annotation2353995
display id: annotation2353995
version: 1
top level: BBa_K1031011
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1031011/annotation2353995/range2353995/1

https://synbiohub.org/public/igem/BBa_K1031011/annotation2353995/range2353995/1
type: Range
persistent identity: range2353995
display id: range2353995
version: 1
direction: direction/forward
top level: BBa_K1031011
start: 94
end: 339
orientation: inline

HP3 3
type: ComponentDefinition
persistent identity: BBa B0101
display id: BBa_B0101
version: 1
was generated by: iGEM to SBOL conversion
description: HP3 3
created: 2007-02-28T12:00:00Z
modified: 2015-08-31T04:07:21Z
mutable provenance: The part was made by primer annealing.
owned by: james
owned by: myers
mutable description: 3' stabilizing mRNA designed by Christina Smolke and Jay Keasling for stabilizing gfp.
discontinued: false
dominant: false
experience: experience/None
group u list: _11_
m user id: 0
owner id: 571
owning group id: 10
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: A single base pair change (A to T at position 25) from the original design was made in order to remove a PstI site occurring in the loop of the hairpin. This should have no effect on the secondary structure.
star: false
creator: Heather Keller
type: DnaRegion
role: SO:0000834
role: partType/RNA
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0101_sequence/1

stem_loop
type: SequenceAnnotation
persistent identity: annotation1919685
display id: annotation1919685
version: 1
top level: HP3 3
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_B0101/annotation1919685/range1919685/1

https://synbiohub.org/public/igem/BBa_B0101/annotation1919685/range1919685/1
type: Range
persistent identity: range1919685
display id: range1919685
version: 1
top level: HP3 3
direction: direction/forward
start: 6
end: 58
orientation: inline

BBa_S03653
type: ComponentDefinition
persistent identity: BBa S03653
display id: BBa_S03653
version: 1
was generated by: iGEM to SBOL conversion
description: E0042:S03650
created: 2007-03-05T12:00:00Z
modified: 2015-05-08T01:14:26Z
owned by: james
owned by: myers
part status: Released HQ 2013
discontinued: false
dominant: false
experience: experience/None
group u list: _10_
m user id: 0
owner id: 571
owning group id: 10
sample status: In stock
status: status/Available
bookmark: false
star: false
creator: Heather Keller
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_B0012
component: BBa_B0010
component: BBa_B0101
component: BBa_E0042
sequence annotation: BBa_B0012
sequence annotation: BBa_B0010
sequence annotation: BBa_E0042
sequence annotation: BBa_B0101
sequence: https://synbiohub.org/public/igem/BBa_S03653_sequence/1

BBa_B0012
type: Component
persistent identity: component1920505
display id: component1920505
version: 1
top level: BBa_S03653
access: public
definition: BBa_B0012

BBa_B0010
type: Component
persistent identity: component1920503
display id: component1920503
version: 1
top level: BBa_S03653
access: public
definition: BBa_B0010

BBa_B0101
type: Component
persistent identity: component1920502
display id: component1920502
version: 1
top level: BBa_S03653
access: public
definition: HP3 3

BBa_E0042
type: Component
persistent identity: component1920500
display id: component1920500
version: 1
top level: BBa_S03653
access: public
definition: BBa_E0042

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1920505
display id: annotation1920505
version: 1
top level: BBa_S03653
location: https://synbiohub.org/public/igem/BBa_S03653/annotation1920505/range1920505/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_S03653/annotation1920505/range1920505/1
type: Range
persistent identity: range1920505
display id: range1920505
version: 1
top level: BBa_S03653
direction: direction/forward
start: 885
end: 925
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1920503
display id: annotation1920503
version: 1
top level: BBa_S03653
location: https://synbiohub.org/public/igem/BBa_S03653/annotation1920503/range1920503/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_S03653/annotation1920503/range1920503/1
type: Range
persistent identity: range1920503
display id: range1920503
version: 1
top level: BBa_S03653
direction: direction/forward
start: 797
end: 876
orientation: inline

BBa_E0042
type: SequenceAnnotation
persistent identity: annotation1920500
display id: annotation1920500
version: 1
top level: BBa_S03653
location: https://synbiohub.org/public/igem/BBa_S03653/annotation1920500/range1920500/1
component: BBa_E0042

https://synbiohub.org/public/igem/BBa_S03653/annotation1920500/range1920500/1
type: Range
persistent identity: range1920500
display id: range1920500
version: 1
top level: BBa_S03653
direction: direction/reverse
start: 1
end: 722
orientation: inline

BBa_B0101
type: SequenceAnnotation
persistent identity: annotation1920502
display id: annotation1920502
version: 1
top level: BBa_S03653
location: https://synbiohub.org/public/igem/BBa_S03653/annotation1920502/range1920502/1
component: BBa_B0101

https://synbiohub.org/public/igem/BBa_S03653/annotation1920502/range1920502/1
type: Range
persistent identity: range1920502
display id: range1920502
version: 1
top level: BBa_S03653
direction: direction/reverse
start: 731
end: 788
orientation: inline

BBa_K1833997
type: ComponentDefinition
persistent identity: BBa K1833997
display id: BBa_K1833997
version: 1
was generated by: iGEM to SBOL conversion
description: T3 promoter as annealed oligonucleotides
created: 2015-09-15T11:00:00Z
modified: 2015-09-16T07:59:14Z
mutable provenance: Synthesized oligonucleotides as described on main page.
owned by: james
owned by: myers
mutable description: This is a part used as a building block for T3 driven expression. However, this part uses a special cloning method with the following synthesized nucleotides: pT3sense: aattcgcggccgcttctagaaattaaccctcactaaagggagaa pT3antisense: ctagttctccctttagtgagggttaatttctagaagcggccgcg The following method can be used to insert pProt before a desired Biobrick part (which should contain an RBS at minimum and preferably a cds and terminator): 1. Cut the desired plasmid with EcoRI and XbaI. Purify the plasmid with a method of your choice (both gel purification and PCR purification kits from Qiagen serve to remove the short undesired oligonucleotide from the digestion). 2. Be sure that your oligos have 5' phosphorylation (treat unphosphorylated oligos with T4 Polynucleotide Kinase). Then, anneal the two oligos by slowly cooling from 98C to room temperature. 3. Finally, ligate the cut plasmid with the annealed oligos by using a 1:10 molar ratio of plasmid to insert. 4. Transform the ligation reaction into E. coli, and plate. 5. Pick colonies, and verify the success of the cloning with sequencing or PCR. (Doing a diagnostic digest is not recommended as the size of the original plasmid and the desired plasmid are very similar.) 6. Add a composite part to the iGEM registry by using this part with the original protein coding part, checking the box to create without a scar, as this part contains a modified scar.
discontinued: false
dominant: false
group u list: _2259_
m user id: 27388
owner id: 27388
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: When ligating with a cut plasmid, this leaves a modified Biobrick scar between the T7 promoter and original Biobrick part, which is included in the sequence of this part.
star: false
creator: Konstantin Borisov
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: Oligo scar
sequence annotation: pT3
sequence: https://synbiohub.org/public/igem/BBa_K1833997_sequence/1

Oligo scar
type: SequenceAnnotation
persistent identity: annotation2460295
display id: annotation2460295
version: 1
top level: BBa_K1833997
role: feature/scar
location: https://synbiohub.org/public/igem/BBa_K1833997/annotation2460295/range2460295/1

https://synbiohub.org/public/igem/BBa_K1833997/annotation2460295/range2460295/1
type: Range
persistent identity: range2460295
display id: range2460295
version: 1
top level: BBa_K1833997
direction: direction/forward
start: 24
end: 29
orientation: inline

pT3
type: SequenceAnnotation
persistent identity: annotation2460294
display id: annotation2460294
version: 1
top level: BBa_K1833997
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1833997/annotation2460294/range2460294/1

https://synbiohub.org/public/igem/BBa_K1833997/annotation2460294/range2460294/1
type: Range
persistent identity: range2460294
display id: range2460294
version: 1
top level: BBa_K1833997
direction: direction/forward
start: 1
end: 23
orientation: inline

BBa_K1833002
type: ComponentDefinition
persistent identity: BBa K1833002
display id: BBa_K1833002
version: 1
was generated by: iGEM to SBOL conversion
description: pT3-GFP
created: 2015-09-15T11:00:00Z
modified: 2015-09-16T07:55:27Z
mutable provenance: The RBS-GFP-terminator was obtained from as part BBa_E0840 from the 2015 Distribution (Plate 2, Well 24D). The T3 promoter was obtained as two short oligonucleotides annealed to form sticky ends. The part containing GFP was then cut with EcoRI and XbaI, and ligated with the annealed oligos to form the completed part.
owned by: james
owned by: myers
mutable description: This part produces GFP in the presence of T3 RNA polymerase. It contains a T3 promoter, a strong RBS, the coding region for GFP, and a double terminator. The GFP is a mutant known as GFPmut3b, and the original citation is as follows: Cormack, B.P., Valdivia, R.H., and S. Falkow. FACS-optimized mutants of green fluorescent protein (GFP). Gene 173: 33-38 (1996). (http://www.sciencedirect.com/science/article/pii/0378111995006850) It has a maximum excitation at 501 nm and maximum emission at 511 nm. For more information, see part BBa_E0040.
discontinued: false
dominant: false
group u list: _2259_
m user id: 27388
owner id: 27388
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: The part containing GFP was cut with EcoRI and XbaI, and ligated with the annealed oligos to form the completed part. Note that due to the choice of cloning technique, a Biobrick scar site remains between the promoter and RBS.
star: false
creator: Konstantin Borisov
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_K1833997
component: BBa_E0840
sequence annotation: BBa_E0840
sequence annotation: BBa_K1833997
sequence: https://synbiohub.org/public/igem/BBa_K1833002_sequence/1

BBa_K1833997
type: Component
persistent identity: component2460312
display id: component2460312
version: 1
top level: BBa_K1833002
access: public
definition: BBa_K1833997

BBa_E0840
type: Component
persistent identity: component2460323
display id: component2460323
version: 1
top level: BBa_K1833002
access: public
definition: GFP genera

BBa_E0840
type: SequenceAnnotation
persistent identity: annotation2460323
display id: annotation2460323
version: 1
top level: BBa_K1833002
location: https://synbiohub.org/public/igem/BBa_K1833002/annotation2460323/range2460323/1
component: BBa_E0840

https://synbiohub.org/public/igem/BBa_K1833002/annotation2460323/range2460323/1
type: Range
persistent identity: range2460323
display id: range2460323
version: 1
top level: BBa_K1833002
direction: direction/reverse
start: 30
end: 907
orientation: inline

BBa_K1833997
type: SequenceAnnotation
persistent identity: annotation2460312
display id: annotation2460312
version: 1
top level: BBa_K1833002
location: https://synbiohub.org/public/igem/BBa_K1833002/annotation2460312/range2460312/1
component: BBa_K1833997

https://synbiohub.org/public/igem/BBa_K1833002/annotation2460312/range2460312/1
type: Range
persistent identity: range2460312
display id: range2460312
version: 1
top level: BBa_K1833002
direction: direction/reverse
start: 1
end: 29
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: stop
sequence annotation: polya
sequence annotation: T7 TE
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

BBa_K1833009
type: ComponentDefinition
persistent identity: BBa K1833009
display id: BBa_K1833009
version: 1
was generated by: iGEM to SBOL conversion
description: T7 promoter -> 434 cI N terminus with P22 repressor specificity
created: 2015-09-17T11:00:00Z
modified: 2015-09-17T11:13:57Z
mutable provenance: "A two-hybrid system based on chimeric operator recognition for studying protein homo/heterodimerization in Escherichia coli" (G. Di Lallo, L. Castagnoli, P. Ghelardini and L. Paolozzi; Microbiology (2001), 147, 1651???1656) described the use of this protein domain.
owned by: james
owned by: myers
mutable description: This part produces the DNA binding domain of the phage 434 cI repressor, with mutations that change the specificity of the DNA to which the protein binds. This protein domain was used as in "A two-hybrid system based on chimeric operator recognition for studying protein homo/heterodimerization in Escherichia coli" (G. Di Lallo, L. Castagnoli, P. Ghelardini and L. Paolozzi; Microbiology (2001), 147, 1651???1656). For more information on the uses of this part, visit 2015.igem.org/Team:Pitt/Protease/Project.
discontinued: false
dominant: false
group u list: _2259_
m user id: 27388
owner id: 27388
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: This part was designed with a T7 promoter so the protein could be overexpressed.
star: false
creator: Konstantin Borisov
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: P22-like 434 cI N-terminus
sequence annotation: pT7
sequence annotation: T7 terminator
sequence annotation: rbs
sequence: https://synbiohub.org/public/igem/BBa_K1833009_sequence/1

P22-like 434 cI N-terminus
type: SequenceAnnotation
persistent identity: annotation2469007
display id: annotation2469007
version: 1
top level: BBa_K1833009
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1833009/annotation2469007/range2469007/1

https://synbiohub.org/public/igem/BBa_K1833009/annotation2469007/range2469007/1
type: Range
persistent identity: range2469007
display id: range2469007
version: 1
top level: BBa_K1833009
direction: direction/forward
start: 33
end: 317
orientation: inline

pT7
type: SequenceAnnotation
persistent identity: annotation2469004
display id: annotation2469004
version: 1
top level: BBa_K1833009
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1833009/annotation2469004/range2469004/1

https://synbiohub.org/public/igem/BBa_K1833009/annotation2469004/range2469004/1
type: Range
persistent identity: range2469004
display id: range2469004
version: 1
top level: BBa_K1833009
direction: direction/forward
start: 1
end: 20
orientation: inline

T7 terminator
type: SequenceAnnotation
persistent identity: annotation2469011
display id: annotation2469011
version: 1
top level: BBa_K1833009
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1833009/annotation2469011/range2469011/1

https://synbiohub.org/public/igem/BBa_K1833009/annotation2469011/range2469011/1
type: Range
persistent identity: range2469011
display id: range2469011
version: 1
top level: BBa_K1833009
direction: direction/forward
start: 318
end: 358
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation2469005
display id: annotation2469005
version: 1
top level: BBa_K1833009
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1833009/annotation2469005/range2469005/1

https://synbiohub.org/public/igem/BBa_K1833009/annotation2469005/range2469005/1
type: Range
persistent identity: range2469005
display id: range2469005
version: 1
top level: BBa_K1833009
direction: direction/forward
start: 24
end: 29
orientation: inline

BBa_K731700
type: ComponentDefinition
persistent identity: BBa K731700
display id: BBa_K731700
version: 1
was generated by: iGEM to SBOL conversion
description: Platform for terminators analysis under the control of T7 promoter
created: 2012-08-13T11:00:00Z
modified: 2015-05-08T01:13:06Z
mutable provenance: It's made from a Roberta Lentini's construct (from Mansy lab) that consist of pET21b with the two proteins and a 20bp linker between them. That construct was mutated two times to eliminate illegal restriction sites; prefix-suffix linker was added by insertion PCR.
owned by: myers
owned by: james
mutable description: We used this part to test terminators: the two subsequent proteins allow to quantify the termination efficiency as the ratio between Venus's and Cherry's fluorescence. The vector is pET21b, that contains lacI, t7 promoter and ampicillin resistance gene. The two fluorescent proteins have partially overlapping emission spectra, thus the fluorimetric measures should be taken with these parameters, to avoid interference between the two: Venus excitation: 485nm, Venus emission: 528nm Cherry excitation: 528nm, Cherry emission: 615nm We inserted the prefix-suffix linker between the two proteins, to allow terminator characterization.
discontinued: false
dominant: false
experience: experience/Works
group u list: _977_
m user id: 0
owner id: 12063
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: Using and modifying this construct is important to remember that the two proteins have strong N-terminal homology, especially designing primers. For any future improvement, maybe it would be better two use two different fluorescence proteins, because these two has partially overlapping emission spectra and different folding kinetics. Using and modifying this construct is important to remember that the two proteins have strong N-terminal homology, especially designing primers. For any future improvement, maybe it would be better two use two different fluorescence proteins, because these two has partially overlapping emission spectra and different folding kinetics.
star: false
creator: Giacomo Giacomelli, Anna Depetris
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: A206K VENUS
sequence annotation: ampicillin resistance gene
sequence annotation: T7 PROMOTER
sequence annotation: lacO
sequence annotation: lacI
sequence annotation: mCHERRY
sequence annotation: T7 TERMINATOR
sequence: https://synbiohub.org/public/igem/BBa_K731700_sequence/1

A206K VENUS
type: SequenceAnnotation
persistent identity: annotation2179556
display id: annotation2179556
version: 1
top level: BBa_K731700
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K731700/annotation2179556/range2179556/1

https://synbiohub.org/public/igem/BBa_K731700/annotation2179556/range2179556/1
type: Range
persistent identity: range2179556
display id: range2179556
version: 1
top level: BBa_K731700
direction: direction/forward
start: 36
end: 752
orientation: inline

ampicillin resistance gene
type: SequenceAnnotation
persistent identity: annotation2179558
display id: annotation2179558
version: 1
top level: BBa_K731700
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K731700/annotation2179558/range2179558/1

https://synbiohub.org/public/igem/BBa_K731700/annotation2179558/range2179558/1
type: Range
persistent identity: range2179558
display id: range2179558
version: 1
top level: BBa_K731700
direction: direction/forward
start: 1711
end: 2370
orientation: inline

T7 PROMOTER
type: SequenceAnnotation
persistent identity: annotation2179560
display id: annotation2179560
version: 1
top level: BBa_K731700
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K731700/annotation2179560/range2179560/1

https://synbiohub.org/public/igem/BBa_K731700/annotation2179560/range2179560/1
type: Range
persistent identity: range2179560
display id: range2179560
version: 1
top level: BBa_K731700
direction: direction/forward
start: 6031
end: 6050
orientation: inline

lacO
type: SequenceAnnotation
persistent identity: annotation2179561
display id: annotation2179561
version: 1
top level: BBa_K731700
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K731700/annotation2179561/range2179561/1

https://synbiohub.org/public/igem/BBa_K731700/annotation2179561/range2179561/1
type: Range
persistent identity: range2179561
display id: range2179561
version: 1
top level: BBa_K731700
direction: direction/forward
start: 6050
end: 6072
orientation: inline

lacI
type: SequenceAnnotation
persistent identity: annotation2179559
display id: annotation2179559
version: 1
top level: BBa_K731700
role: feature/protein
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K731700/annotation2179559/range2179559/1

https://synbiohub.org/public/igem/BBa_K731700/annotation2179559/range2179559/1
type: Range
persistent identity: range2179559
display id: range2179559
version: 1
top level: BBa_K731700
direction: direction/reverse
start: 4562
end: 5653
orientation: inline

mCHERRY
type: SequenceAnnotation
persistent identity: annotation2179562
display id: annotation2179562
version: 1
top level: BBa_K731700
role: feature/protein
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K731700/annotation2179562/range2179562/1

https://synbiohub.org/public/igem/BBa_K731700/annotation2179562/range2179562/1
type: Range
persistent identity: range2179562
display id: range2179562
version: 1
top level: BBa_K731700
direction: direction/forward
start: 6119
end: 6829
orientation: inline

T7 TERMINATOR
type: SequenceAnnotation
persistent identity: annotation2179557
display id: annotation2179557
version: 1
top level: BBa_K731700
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K731700/annotation2179557/range2179557/1

https://synbiohub.org/public/igem/BBa_K731700/annotation2179557/range2179557/1
type: Range
persistent identity: range2179557
display id: range2179557
version: 1
top level: BBa_K731700
direction: direction/forward
start: 843
end: 890
orientation: inline

BBa_K1833000
type: ComponentDefinition
persistent identity: BBa K1833000
display id: BBa_K1833000
version: 1
was generated by: iGEM to SBOL conversion
description: pT7-eGFP
created: 2015-09-12T11:00:00Z
modified: 2015-09-18T11:04:36Z
mutable provenance: The RBS-GFP-terminator was obtained from as part BBa_E0840 from the 2015 Distribution (Plate 2, Well 24D). The T7 promoter was obtained as two short oligonucleotides annealed to form sticky ends. The part containing GFP was then cut with EcoRI and XbaI, and ligated with the annealed oligos to form the completed part. Note that due to the choice of technique, a Biobrick scar site remains between the promoter and RBS.
owned by: james
owned by: myers
mutable description: This part produces GFP in the presence of T7 RNA polymerase. It contains a T7 promoter, a strong RBS, the coding region for GFP, and a double terminator. The GFP is a mutant known as GFPmut3b, and the original citation is as follows: Cormack, B.P., Valdivia, R.H., and S. Falkow. FACS-optimized mutants of green fluorescent protein (GFP). Gene 173: 33-38 (1996). (http://www.sciencedirect.com/science/article/pii/0378111995006850) It has a maximum excitation at 501 nm and maximum emission at 511 nm. For more information, see part BBa_E0040
discontinued: false
dominant: false
experience: experience/None
group u list: _2259_
m user id: 27388
owner id: 27388
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: The part containing GFP was cut with EcoRI and XbaI, and ligated with the annealed oligos to form the completed part. Note that due to the choice of technique, a Biobrick scar site remains between the promoter and RBS.
star: false
creator: Konstantin Borisov
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_K1833999
component: BBa_E0840
sequence annotation: BBa_E0840
sequence annotation: BBa_K1833999
sequence: https://synbiohub.org/public/igem/BBa_K1833000_sequence/1

BBa_K1833999
type: Component
persistent identity: component2460119
display id: component2460119
version: 1
top level: BBa_K1833000
access: public
definition: BBa_K1833999

BBa_E0840
type: Component
persistent identity: component2460130
display id: component2460130
version: 1
top level: BBa_K1833000
access: public
definition: GFP genera

BBa_E0840
type: SequenceAnnotation
persistent identity: annotation2460130
display id: annotation2460130
version: 1
top level: BBa_K1833000
location: https://synbiohub.org/public/igem/BBa_K1833000/annotation2460130/range2460130/1
component: BBa_E0840

https://synbiohub.org/public/igem/BBa_K1833000/annotation2460130/range2460130/1
type: Range
persistent identity: range2460130
display id: range2460130
version: 1
top level: BBa_K1833000
direction: direction/reverse
start: 30
end: 907
orientation: inline

BBa_K1833999
type: SequenceAnnotation
persistent identity: annotation2460119
display id: annotation2460119
version: 1
top level: BBa_K1833000
location: https://synbiohub.org/public/igem/BBa_K1833000/annotation2460119/range2460119/1
component: BBa_K1833999

https://synbiohub.org/public/igem/BBa_K1833000/annotation2460119/range2460119/1
type: Range
persistent identity: range2460119
display id: range2460119
version: 1
top level: BBa_K1833000
direction: direction/reverse
start: 1
end: 29
orientation: inline

T7Gene6
type: ComponentDefinition
persistent identity: BBa K093001
display id: BBa_K093001
version: 1
was generated by: iGEM to SBOL conversion
description: T7 Gene 6 exonuclease
created: 2008-10-25T11:00:00Z
modified: 2015-05-08T01:08:40Z
mutable provenance: T7 genomic DNA
owned by: myers
owned by: james
mutable description: This is the T7 gene 6 ORF, that codes for the T7 gene 6 5'-3' exonuclease. For properties of T7 gene 6 exonuclease see: Kerr and Sadowski "Gene 6 Exonuclease of Bacteriophage T7" The Journal of Biological Chemistry Vol. 247 No. 1 January 10, pp 311-318, 1972.
discontinued: false
dominant: false
experience: experience/None
group u list: _247_
m user id: 0
owner id: 3630
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: We needed to change the stop codon to the standard stop codon TAA. We later realized that we should have added tandem TAA. The Tandem TAA stop codon is present in the composite parts containing this ORF. The ORF alone has the single TAA stop codon.
star: false
creator: John Heil, Shira Davis, Danielle Nash
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K093001_sequence/1

BBa_K2084004
type: ComponentDefinition
persistent identity: BBa K2084004
display id: BBa_K2084004
version: 1
was generated by: iGEM to SBOL conversion
description: T3 Promoter with RBS site
created: 2016-10-13T11:00:00Z
modified: 2016-10-29T09:48:58Z
mutable provenance: The T3 promoter comes from the T3 bacteriophage. The RBS site comes from...
owned by: james
owned by: myers
mutable description: This is the T3 promoter with an RBS site added. Other groups should use this part to regulate their part by adding it in front.
discontinued: false
dominant: false
experience: experience/None
group u list: _2552_
m user id: 32066
owner id: 32131
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: NA
star: false
creator: Maya Lemmon-Kishi
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
component: BBa_K2084000
component: BBa_B0034
sequence annotation: BBa_B0034
sequence annotation: BBa_K2084000
sequence: https://synbiohub.org/public/igem/BBa_K2084004_sequence/1

BBa_K2084000
type: Component
persistent identity: component2531295
display id: component2531295
version: 1
top level: BBa_K2084004
access: public
definition: BBa_K2084000

BBa_B0034
type: Component
persistent identity: component2531297
display id: component2531297
version: 1
top level: BBa_K2084004
access: public
definition: BBa_B0034

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2531297
display id: annotation2531297
version: 1
top level: BBa_K2084004
location: https://synbiohub.org/public/igem/BBa_K2084004/annotation2531297/range2531297/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K2084004/annotation2531297/range2531297/1
type: Range
persistent identity: range2531297
display id: range2531297
version: 1
top level: BBa_K2084004
direction: direction/reverse
start: 32
end: 43
orientation: inline

BBa_K2084000
type: SequenceAnnotation
persistent identity: annotation2531295
display id: annotation2531295
version: 1
top level: BBa_K2084004
location: https://synbiohub.org/public/igem/BBa_K2084004/annotation2531295/range2531295/1
component: BBa_K2084000

https://synbiohub.org/public/igem/BBa_K2084004/annotation2531295/range2531295/1
type: Range
persistent identity: range2531295
display id: range2531295
version: 1
top level: BBa_K2084004
direction: direction/reverse
start: 1
end: 23
orientation: inline

BBa_Z0253
type: ComponentDefinition
persistent identity: BBa Z0253
display id: BBa_Z0253
version: 1
was generated by: iGEM to SBOL conversion
description: T7 weak binding promoter
created: 2006-05-15T11:00:00Z
modified: 2015-05-08T01:14:55Z
owned by: james
owned by: myers
mutable description: -- No description --
discontinued: false
dominant: false
experience: experience/None
group u list: _11_10_
m user id: 0
owner id: 64
owning group id: 10
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Sriram Kosuri
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: ag->GA from concensus
sequence annotation: ag->TA from concensus
sequence: https://synbiohub.org/public/igem/BBa_Z0253_sequence/1

ag->GA from concensus
type: SequenceAnnotation
persistent identity: annotation1862691
display id: annotation1862691
version: 1
top level: BBa_Z0253
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_Z0253/annotation1862691/range1862691/1

https://synbiohub.org/public/igem/BBa_Z0253/annotation1862691/range1862691/1
type: Range
persistent identity: range1862691
display id: range1862691
version: 1
top level: BBa_Z0253
direction: direction/forward
start: 31
end: 32
orientation: inline

ag->TA from concensus
type: SequenceAnnotation
persistent identity: annotation1862684
display id: annotation1862684
version: 1
top level: BBa_Z0253
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_Z0253/annotation1862684/range1862684/1

https://synbiohub.org/public/igem/BBa_Z0253/annotation1862684/range1862684/1
type: Range
persistent identity: range1862684
display id: range1862684
version: 1
top level: BBa_Z0253
direction: direction/forward
start: 27
end: 28
orientation: inline

BBa_K921002
type: ComponentDefinition
persistent identity: BBa K921002
display id: BBa_K921002
version: 1
was generated by: iGEM to SBOL conversion
description: T7 RNAP + IPTG->PoPs (Mutant III)
created: 2012-09-30T11:00:00Z
modified: 2015-05-08T01:13:46Z
mutable provenance: The part consists of a sequences that resemble a T7 promoter and the lacO sequence found in E. coli.
owned by: james
owned by: myers
mutable description: This is a mutant T7 promoter that includes a lacO sequence. This is repressible by the LacI protein and is induced in the presence of IPTG. The promoter sits in the 5' region of the gene of interest and initiates transcription when the cell expresses T7 RNA polymerase. Be sure you are using a cell strain that includes this gene (DE3).
discontinued: false
dominant: false
experience: experience/Works
group u list: _1186_
m user id: 0
owner id: 12713
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: There are three portions to the T7 promoter: the recognition site, the melting box (or TATA box) and the initation site. Mutations in these sequences caused differential behavior from the wild type.
star: false
creator: Eric Pederson
type: DnaRegion
role: partType/T7
role: SO:0001207
sequence annotation: LacO
sequence annotation: Initiation
sequence annotation: T7 Promoter
sequence: https://synbiohub.org/public/igem/BBa_K921002_sequence/1

LacO
type: SequenceAnnotation
persistent identity: annotation2205317
display id: annotation2205317
version: 1
top level: BBa_K921002
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K921002/annotation2205317/range2205317/1

https://synbiohub.org/public/igem/BBa_K921002/annotation2205317/range2205317/1
type: Range
persistent identity: range2205317
display id: range2205317
version: 1
top level: BBa_K921002
direction: direction/forward
start: 19
end: 47
orientation: inline

Initiation
type: SequenceAnnotation
persistent identity: annotation2205318
display id: annotation2205318
version: 1
top level: BBa_K921002
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K921002/annotation2205318/range2205318/1

https://synbiohub.org/public/igem/BBa_K921002/annotation2205318/range2205318/1
type: Range
persistent identity: range2205318
display id: range2205318
version: 1
top level: BBa_K921002
direction: direction/forward
start: 18
end: 20
orientation: inline

T7 Promoter
type: SequenceAnnotation
persistent identity: annotation2205316
display id: annotation2205316
version: 1
top level: BBa_K921002
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K921002/annotation2205316/range2205316/1

https://synbiohub.org/public/igem/BBa_K921002/annotation2205316/range2205316/1
type: Range
persistent identity: range2205316
display id: range2205316
version: 1
top level: BBa_K921002
direction: direction/forward
start: 1
end: 20
orientation: inline

BBa_K2084000
type: ComponentDefinition
persistent identity: BBa K2084000
display id: BBa_K2084000
version: 1
was generated by: iGEM to SBOL conversion
description: T3 Promoter
created: 2016-10-11T11:00:00Z
modified: 2016-10-29T09:13:21Z
mutable provenance: Wild-type found in T3 bacteriophage. Synthesized by IDT.
owned by: james
owned by: myers
mutable description: Promoter for the T3 RNA Polymerase.
discontinued: false
dominant: false
experience: experience/None
group u list: _2552_
m user id: 32066
owner id: 32131
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: NA
star: false
creator: Maya Lemmon-Kishi
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K2084000_sequence/1

BBa_R0184
type: ComponentDefinition
persistent identity: BBa R0184
display id: BBa_R0184
version: 1
was generated by: iGEM to SBOL conversion
description: T7 promoter (lacI repressible)
created: 2007-04-25T11:00:00Z
modified: 2015-05-08T01:14:15Z
mutable provenance: This sequence is based on the T7lac promoter described by Dubendorff and Studier (JMB 1991, 219, 45-59). The only modification is the mutation at -16.
owned by: james
owned by: myers
mutable description: A mutant version of the T7lac promoter designed by Dubendorff and Studier. The promoter is based on the strong ??10 T7 promoter but incorporates an A->C mutation at -16. The LacI operator site is a partially symmetric 25bp sequence.
discontinued: false
dominant: false
experience: experience/None
group u list: _11_
m user id: 0
owner id: 135
owning group id: 84
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: The -16 mutation was included to weaken the consensus promoter as described by Imburgio and co-workers (see BBa_R0183 for more information)
star: false
creator: Bartholomew Canton
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: Center of binding site symmetry
sequence annotation: lacI binding site
sequence annotation: BBa_R0183 fragment
sequence: https://synbiohub.org/public/igem/BBa_R0184_sequence/1

Center of binding site symmetry
type: SequenceAnnotation
persistent identity: annotation1932680
display id: annotation1932680
version: 1
top level: BBa_R0184
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_R0184/annotation1932680/range1932680/1

https://synbiohub.org/public/igem/BBa_R0184/annotation1932680/range1932680/1
type: Range
persistent identity: range1932680
display id: range1932680
version: 1
top level: BBa_R0184
direction: direction/forward
start: 31
end: 31
orientation: inline

lacI binding site
type: SequenceAnnotation
persistent identity: annotation1932679
display id: annotation1932679
version: 1
top level: BBa_R0184
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_R0184/annotation1932679/range1932679/1

https://synbiohub.org/public/igem/BBa_R0184/annotation1932679/range1932679/1
type: Range
persistent identity: range1932679
display id: range1932679
version: 1
top level: BBa_R0184
direction: direction/forward
start: 20
end: 44
orientation: inline

BBa_R0183 fragment
type: SequenceAnnotation
persistent identity: annotation1932678
display id: annotation1932678
version: 1
top level: BBa_R0184
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0184/annotation1932678/range1932678/1

https://synbiohub.org/public/igem/BBa_R0184/annotation1932678/range1932678/1
type: Range
persistent identity: range1932678
display id: range1932678
version: 1
top level: BBa_R0184
direction: direction/forward
start: 1
end: 20
orientation: inline

BBa_E0041
type: ComponentDefinition
persistent identity: BBa E0041
display id: BBa_E0041
version: 1
was generated by: iGEM to SBOL conversion
description: SapI + GFP
created: 2007-03-05T12:00:00Z
modified: 2015-08-31T04:07:25Z
mutable provenance: The part was generated by PCR amplification of part E0040 with the SapI site incorported into the forward promoter.
owned by: james
owned by: myers
mutable description: GFP (mut3) part number E0040 with a unpstream SapI site. Cleave with SapI results in an overhang of 3'-TAC - 5' from the left end of the GFP coding region, making the coding sequence compatible with Heather Keller's alternative assembly method for blunt cloning of RBS sequences upstream of a coding sequence.
discontinued: false
dominant: false
group u list: _11_
m user id: 0
owner id: 571
owning group id: 10
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: n/a
star: false
creator: Heather Keller
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: BBa_B0102
sequence annotation: SapI recognition
sequence annotation: BBa_E0040
sequence: https://synbiohub.org/public/igem/BBa_E0041_sequence/1

BBa_B0102
type: SequenceAnnotation
persistent identity: annotation1920211
display id: annotation1920211
version: 1
top level: BBa_E0041
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_E0041/annotation1920211/range1920211/1

https://synbiohub.org/public/igem/BBa_E0041/annotation1920211/range1920211/1
type: Range
persistent identity: range1920211
display id: range1920211
version: 1
top level: BBa_E0041
direction: direction/reverse
start: 1
end: 8
orientation: inline

SapI recognition
type: SequenceAnnotation
persistent identity: annotation1920210
display id: annotation1920210
version: 1
top level: BBa_E0041
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_E0041/annotation1920210/range1920210/1

https://synbiohub.org/public/igem/BBa_E0041/annotation1920210/range1920210/1
type: Range
persistent identity: range1920210
display id: range1920210
version: 1
top level: BBa_E0041
direction: direction/forward
start: 1
end: 7
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation1920212
display id: annotation1920212
version: 1
top level: BBa_E0041
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_E0041/annotation1920212/range1920212/1

https://synbiohub.org/public/igem/BBa_E0041/annotation1920212/range1920212/1
type: Range
persistent identity: range1920212
display id: range1920212
version: 1
top level: BBa_E0041
direction: direction/reverse
start: 9
end: 728
orientation: inline

BBa_K921000
type: ComponentDefinition
persistent identity: BBa K921000
display id: BBa_K921000
version: 1
was generated by: iGEM to SBOL conversion
description: T7 RNAP + IPTG->PoPs (Mutant I)
created: 2012-09-30T11:00:00Z
modified: 2015-05-08T01:13:46Z
mutable provenance: The part consists of a sequences that resemble a T7 promoter and the lacO sequence found in E. coli.
owned by: james
owned by: myers
mutable description: This is a mutant T7 promoter that includes a lacO sequence. This is repressible by the LacI protein and is induced in the presence of IPTG. The promoter sits in the 5' region of the gene of interest and initiates transcription when the cell expresses T7 RNA polymerase. Be sure you are using a cell strain that includes this gene (DE3).
discontinued: false
dominant: false
experience: experience/Works
group u list: _1186_
m user id: 0
owner id: 12713
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable notes: There are three portions to the T7 promoter: the recognition site, the melting box (or TATA box) and the initation site. Mutations in these sequences caused differential behavior from the wild type.
star: false
creator: Eric Pederson
type: DnaRegion
role: partType/T7
role: SO:0001207
sequence annotation: LacO
sequence annotation: Initiation
sequence annotation: T7 promoter
sequence: https://synbiohub.org/public/igem/BBa_K921000_sequence/1

LacO
type: SequenceAnnotation
persistent identity: annotation2205290
display id: annotation2205290
version: 1
top level: BBa_K921000
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K921000/annotation2205290/range2205290/1

https://synbiohub.org/public/igem/BBa_K921000/annotation2205290/range2205290/1
type: Range
persistent identity: range2205290
display id: range2205290
version: 1
top level: BBa_K921000
direction: direction/forward
start: 19
end: 46
orientation: inline

Initiation
type: SequenceAnnotation
persistent identity: annotation2205291
display id: annotation2205291
version: 1
top level: BBa_K921000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K921000/annotation2205291/range2205291/1

https://synbiohub.org/public/igem/BBa_K921000/annotation2205291/range2205291/1
type: Range
persistent identity: range2205291
display id: range2205291
version: 1
top level: BBa_K921000
direction: direction/forward
start: 19
end: 20
orientation: inline

T7 promoter
type: SequenceAnnotation
persistent identity: annotation2205289
display id: annotation2205289
version: 1
top level: BBa_K921000
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K921000/annotation2205289/range2205289/1

https://synbiohub.org/public/igem/BBa_K921000/annotation2205289/range2205289/1
type: Range
persistent identity: range2205289
display id: range2205289
version: 1
top level: BBa_K921000
direction: direction/forward
start: 1
end: 19
orientation: inline

GFP
type: ComponentDefinition
persistent identity: BBa E0040
display id: BBa_E0040
version: 1
was generated by: iGEM to SBOL conversion
description: green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212
created: 2004-09-29T11:00:00Z
modified: 2016-01-26T02:09:38Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: GFP (mut3b) [note that this part does not have a barcode]
discontinued: false
dominant: true
experience: experience/Works
group u list: _11_1_
m user id: 4206
owner id: 61
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: jcbraff
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: GFP protein
sequence: https://synbiohub.org/public/igem/BBa_E0040_sequence/1

GFP protein
type: SequenceAnnotation
persistent identity: annotation1934520
display id: annotation1934520
version: 1
top level: GFP
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_E0040/annotation1934520/range1934520/1

https://synbiohub.org/public/igem/BBa_E0040/annotation1934520/range1934520/1
type: Range
persistent identity: range1934520
display id: range1934520
version: 1
top level: GFP
direction: direction/forward
start: 1
end: 720
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

BBa_R0183
type: ComponentDefinition
persistent identity: BBa R0183
display id: BBa_R0183
version: 1
was generated by: iGEM to SBOL conversion
description: T7 RNAP promoter
created: 2005-09-25T11:00:00Z
modified: 2015-05-08T01:14:15Z
owned by: james
owned by: myers
mutable description: T7 promoter with an A->C mutation at the -16 position of the consensus sequence
discontinued: false
dominant: false
experience: experience/None
group u list: _11_6_
m user id: 0
owner id: 135
owning group id: 6
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Barry Canton
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_R0183_sequence/1

P3
type: ComponentDefinition
persistent identity: BBa K1781000
display id: BBa_K1781000
version: 1
was generated by: iGEM to SBOL conversion
description: P3 from M13
creator: Marvin Prein
created: 2015-09-06T11:00:00Z
modified: 2015-09-11T01:17:28Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2206_
m user id: 25533
owner id: 25533
owning group id: 9
sample status: No part sequence
status: status/Planning
bookmark: false
mutable description: will be edited
mutable notes: will be edited
mutable provenance: will be edited
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: P3
sequence: https://synbiohub.org/public/igem/BBa_K1781000_sequence/1

P3
type: SequenceAnnotation
persistent identity: annotation2448988
display id: annotation2448988
version: 1
top level: P3
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1781000/annotation2448988/range2448988/1

https://synbiohub.org/public/igem/BBa_K1781000/annotation2448988/range2448988/1
type: Range
persistent identity: range2448988
display id: range2448988
version: 1
direction: direction/forward
top level: P3
start: 1
end: 1302
orientation: inline

BBa_K113012
type: ComponentDefinition
persistent identity: BBa K113012
display id: BBa_K113012
version: 1
was generated by: iGEM to SBOL conversion
description: weaken overlapping T7 promoter
created: 2008-10-25T11:00:00Z
modified: 2015-05-08T01:09:20Z
mutable provenance: The sequence of T7 promoter is TAATACGACTCACTATA (from 5' to 3').
owned by: james
owned by: myers
mutable description: Two T7 promoters are reversely overlapping in two base pairs while a point mutation occurs at the 12th base pair, from A-T to T-A.
discontinued: false
dominant: false
experience: experience/None
group u list: _254_
m user id: 0
owner id: 2711
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: It is difficult to make this short DNA by PCR as both sides are T7 promoters, which are very similar. It is highly possible that they form loops before they can be synthesized by PCR. Thus we order two single stranded DNA and then anneal them together by touchdown PCR.
star: false
creator: Wu Jingjing, Wang Jinyu
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: T7 promoter
sequence: https://synbiohub.org/public/igem/BBa_K113012_sequence/1

T7 promoter
type: SequenceAnnotation
persistent identity: annotation1989400
display id: annotation1989400
version: 1
top level: BBa_K113012
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K113012/annotation1989400/range1989400/1

https://synbiohub.org/public/igem/BBa_K113012/annotation1989400/range1989400/1
type: Range
persistent identity: range1989400
display id: range1989400
version: 1
top level: BBa_K113012
direction: direction/reverse
start: 4
end: 21
orientation: inline

T7 promoter
type: SequenceAnnotation
persistent identity: annotation1989402
display id: annotation1989402
version: 1
top level: BBa_K113012
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K113012/annotation1989402/range1989402/1

https://synbiohub.org/public/igem/BBa_K113012/annotation1989402/range1989402/1
type: Range
persistent identity: range1989402
display id: range1989402
version: 1
top level: BBa_K113012
direction: direction/forward
start: 20
end: 37
orientation: inline

BBa_R0186
type: ComponentDefinition
persistent identity: BBa R0186
display id: BBa_R0186
version: 1
was generated by: iGEM to SBOL conversion
description: T7 promoter (lacI repressible)
created: 2008-04-07T11:00:00Z
modified: 2015-05-08T01:14:15Z
mutable provenance: This sequence is based on the T7lac promoter described by Dubendorff and Studier (JMB 1991, 219, 45-59). The only modification is the mutation at -9.
owned by: james
owned by: myers
mutable description: A lac repressible T7 promoter. This promoter is similar to those found in the pET vectors with T7lac promoters. I mutated the -9 base to an A, which should make it less than 3% of the strength of consensus. The base numbering is as used by Studier and coworkers (JMB 1991, 219, 45-59). See BBa_R0183 for more information.
discontinued: false
dominant: false
experience: experience/None
group u list: _11_
m user id: 0
owner id: 135
owning group id: 84
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: The -9 mutation from consensus was included to weaken the consensus promoter as described by Imburgio and co-workers (see BBa_R0183 for more information)
star: false
creator: Bartholomew Canton
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: LacI binding site
sequence annotation: T7 promoter
sequence: https://synbiohub.org/public/igem/BBa_R0186_sequence/1

LacI binding site
type: SequenceAnnotation
persistent identity: annotation1962094
display id: annotation1962094
version: 1
top level: BBa_R0186
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0186/annotation1962094/range1962094/1

https://synbiohub.org/public/igem/BBa_R0186/annotation1962094/range1962094/1
type: Range
persistent identity: range1962094
display id: range1962094
version: 1
top level: BBa_R0186
direction: direction/forward
start: 20
end: 44
orientation: inline

T7 promoter
type: SequenceAnnotation
persistent identity: annotation1962095
display id: annotation1962095
version: 1
top level: BBa_R0186
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0186/annotation1962095/range1962095/1

https://synbiohub.org/public/igem/BBa_R0186/annotation1962095/range1962095/1
type: Range
persistent identity: range1962095
display id: range1962095
version: 1
top level: BBa_R0186
direction: direction/forward
start: 1
end: 20
orientation: inline

BBa_S03651
type: ComponentDefinition
persistent identity: BBa S03651
display id: BBa_S03651
version: 1
was generated by: iGEM to SBOL conversion
description: S00159:P1010
created: 2007-03-05T12:00:00Z
modified: 2015-06-15T12:38:52Z
owned by: james
owned by: myers
discontinued: true
dominant: false
experience: experience/None
group u list: _10_
m user id: 4206
owner id: 571
owning group id: 10
sample status: It's complicated
status: status/Deleted
bookmark: false
star: false
creator: Heather Keller
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_S00159
component: BBa_P1010
sequence annotation: BBa_S00159
sequence annotation: BBa_P1010
sequence: https://synbiohub.org/public/igem/BBa_S03651_sequence/1

BBa_S00159
type: Component
persistent identity: component1920355
display id: component1920355
version: 1
top level: BBa_S03651
access: public
definition: BBa_S00159

BBa_P1010
type: Component
persistent identity: component1920361
display id: component1920361
version: 1
top level: BBa_S03651
access: public
definition: ccdB

BBa_S00159
type: SequenceAnnotation
persistent identity: annotation1920355
display id: annotation1920355
version: 1
top level: BBa_S03651
location: https://synbiohub.org/public/igem/BBa_S03651/annotation1920355/range1920355/1
component: BBa_S00159

https://synbiohub.org/public/igem/BBa_S03651/annotation1920355/range1920355/1
type: Range
persistent identity: range1920355
display id: range1920355
version: 1
top level: BBa_S03651
direction: direction/reverse
start: 1
end: 176
orientation: inline

BBa_P1010
type: SequenceAnnotation
persistent identity: annotation1920361
display id: annotation1920361
version: 1
top level: BBa_S03651
location: https://synbiohub.org/public/igem/BBa_S03651/annotation1920361/range1920361/1
component: BBa_P1010

https://synbiohub.org/public/igem/BBa_S03651/annotation1920361/range1920361/1
type: Range
persistent identity: range1920361
display id: range1920361
version: 1
top level: BBa_S03651
direction: direction/reverse
start: 185
end: 859
orientation: inline

T7
type: ComponentDefinition
persistent identity: BBa G00113
display id: BBa_G00113
version: 1
was generated by: iGEM to SBOL conversion
description: T7 promoter sequencing primer, 20-mer
created: 2008-10-16T11:00:00Z
modified: 2015-08-31T04:07:28Z
mutable provenance: T7 promoter.
owned by: james
owned by: myers
mutable description: This primer anneals to the T7 Promoter region of any vector containing the T7 Promoter.
discontinued: false
dominant: true
experience: experience/Works
group u list: _41_
m user id: 0
owner id: 126
owning group id: 162
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: <biblio> #Wallace pmid=6282692 </biblio>
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000112
role: partType/Primer
sequence: https://synbiohub.org/public/igem/BBa_G00113_sequence/1

BBa_R0180
type: ComponentDefinition
persistent identity: BBa R0180
display id: BBa_R0180
version: 1
was generated by: iGEM to SBOL conversion
description: T7 RNAP promoter
created: 2005-09-25T11:00:00Z
modified: 2015-05-08T01:14:15Z
owned by: james
owned by: myers
mutable description: T7 promoter with an A->T mutation at the -16 position of the consensus sequence
discontinued: false
dominant: false
experience: experience/None
group u list: _11_6_
m user id: 0
owner id: 135
owning group id: 6
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Barry Canton
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_R0180_sequence/1

GFP genera
type: ComponentDefinition
persistent identity: BBa E0840
display id: BBa_E0840
version: 1
was generated by: iGEM to SBOL conversion
description: GFP generator
created: 2004-10-17T11:00:00Z
modified: 2015-08-31T04:07:26Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: B0030.E0040.B0015
discontinued: false
dominant: true
experience: experience/Works
group u list: _11_1_
m user id: 0
owner id: 61
owning group id: 7
sample status: In stock
status: status/Available
bookmark: true
star: true
creator: Jennifer Braff
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_B0030
component: BBa_B0010
component: BBa_E0040
component: BBa_B0012
sequence annotation: BBa_B0030
sequence annotation: BBa_B0012
sequence annotation: BBa_E0040
sequence annotation: BBa_B0010
sequence: https://synbiohub.org/public/igem/BBa_E0840_sequence/1

BBa_B0030
type: Component
persistent identity: component1249239
display id: component1249239
version: 1
top level: GFP genera
access: public
definition: BBa_B0030

BBa_B0010
type: Component
persistent identity: component1249247
display id: component1249247
version: 1
top level: GFP genera
access: public
definition: BBa_B0010

BBa_E0040
type: Component
persistent identity: component1249242
display id: component1249242
version: 1
top level: GFP genera
access: public
definition: GFP

BBa_B0012
type: Component
persistent identity: component1249257
display id: component1249257
version: 1
top level: GFP genera
access: public
definition: BBa_B0012

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation1249239
display id: annotation1249239
version: 1
top level: GFP genera
location: https://synbiohub.org/public/igem/BBa_E0840/annotation1249239/range1249239/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_E0840/annotation1249239/range1249239/1
type: Range
persistent identity: range1249239
display id: range1249239
version: 1
top level: GFP genera
direction: direction/forward
start: 1
end: 15
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1249257
display id: annotation1249257
version: 1
top level: GFP genera
location: https://synbiohub.org/public/igem/BBa_E0840/annotation1249257/range1249257/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_E0840/annotation1249257/range1249257/1
type: Range
persistent identity: range1249257
display id: range1249257
version: 1
top level: GFP genera
direction: direction/forward
start: 838
end: 878
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation1249242
display id: annotation1249242
version: 1
top level: GFP genera
location: https://synbiohub.org/public/igem/BBa_E0840/annotation1249242/range1249242/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_E0840/annotation1249242/range1249242/1
type: Range
persistent identity: range1249242
display id: range1249242
version: 1
top level: GFP genera
direction: direction/forward
start: 22
end: 741
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1249247
display id: annotation1249247
version: 1
top level: GFP genera
location: https://synbiohub.org/public/igem/BBa_E0840/annotation1249247/range1249247/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_E0840/annotation1249247/range1249247/1
type: Range
persistent identity: range1249247
display id: range1249247
version: 1
top level: GFP genera
direction: direction/forward
start: 750
end: 829
orientation: inline

BBa_E0042
type: ComponentDefinition
persistent identity: BBa E0042
display id: BBa_E0042
version: 1
was generated by: iGEM to SBOL conversion
description: SapI + mCherry
created: 2007-03-05T12:00:00Z
modified: 2015-08-31T04:07:25Z
mutable provenance: The part was generated by PCR amplification of part E0040 with the SapI site incorported into the forward promoter.
owned by: james
owned by: myers
mutable description: mCherry part number J06504 with an upstream SapI site. Cleave with SapI results in an overhang of 3'-TAC - 5' from the left end of the mCherry coding region, making the coding sequence compatible with Heather Keller's alternative assembly method for blunt cloning of RBS sequences upstream of a coding sequence.
discontinued: false
dominant: false
group u list: _11_
m user id: 0
owner id: 571
owning group id: 10
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: n/a
star: false
creator: Heather Keller
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: C->T (removing PstI site)
sequence annotation: BBa_B0102
sequence annotation: mCherry
sequence annotation: SapI recognition
sequence annotation: BBa_J06504
sequence: https://synbiohub.org/public/igem/BBa_E0042_sequence/1

C->T (removing PstI site)
type: SequenceAnnotation
persistent identity: annotation1920226
display id: annotation1920226
version: 1
top level: BBa_E0042
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_E0042/annotation1920226/range1920226/1

https://synbiohub.org/public/igem/BBa_E0042/annotation1920226/range1920226/1
type: Range
persistent identity: range1920226
display id: range1920226
version: 1
top level: BBa_E0042
direction: direction/forward
start: 360
end: 360
orientation: inline

BBa_B0102
type: SequenceAnnotation
persistent identity: annotation1920224
display id: annotation1920224
version: 1
top level: BBa_E0042
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_E0042/annotation1920224/range1920224/1

https://synbiohub.org/public/igem/BBa_E0042/annotation1920224/range1920224/1
type: Range
persistent identity: range1920224
display id: range1920224
version: 1
top level: BBa_E0042
direction: direction/reverse
start: 1
end: 8
orientation: inline

mCherry
type: SequenceAnnotation
persistent identity: annotation1920225
display id: annotation1920225
version: 1
top level: BBa_E0042
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_E0042/annotation1920225/range1920225/1

https://synbiohub.org/public/igem/BBa_E0042/annotation1920225/range1920225/1
type: Range
persistent identity: range1920225
display id: range1920225
version: 1
top level: BBa_E0042
direction: direction/forward
start: 9
end: 719
orientation: inline

SapI recognition
type: SequenceAnnotation
persistent identity: annotation1920223
display id: annotation1920223
version: 1
top level: BBa_E0042
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_E0042/annotation1920223/range1920223/1

https://synbiohub.org/public/igem/BBa_E0042/annotation1920223/range1920223/1
type: Range
persistent identity: range1920223
display id: range1920223
version: 1
top level: BBa_E0042
direction: direction/forward
start: 1
end: 7
orientation: inline

BBa_J06504
type: SequenceAnnotation
persistent identity: annotation1920227
display id: annotation1920227
version: 1
top level: BBa_E0042
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_E0042/annotation1920227/range1920227/1

https://synbiohub.org/public/igem/BBa_E0042/annotation1920227/range1920227/1
type: Range
persistent identity: range1920227
display id: range1920227
version: 1
top level: BBa_E0042
direction: direction/reverse
start: 9
end: 722
orientation: inline

BBa_S00159
type: ComponentDefinition
persistent identity: BBa S00159
display id: BBa_S00159
version: 1
was generated by: iGEM to SBOL conversion
description: R0011+B0100+B0048
created: 2007-03-05T12:00:00Z
modified: 2015-05-08T01:14:16Z
mutable provenance: This part was generated by PCR amplification. The upstream biobricks cut sites (EcoRI and XbaI) as well as the R0011 promoter were incorporated into the forward promoter recognizing the ompA region in the MG1655 genome. The AvrII cut site, as well as the downstream biobricks cut sites (SpeI and PstI) were incorporated into the reverse promoter.
owned by: james
owned by: myers
mutable description: This part is an intermediate assembly product that contains the R0011 promoter (lambda cI promoter with lacI binding sites), the OmpaA 5' UTR containing a double hairpin for transcript stability, and an AvrII restriction site to facilitate later downstream cloning of an RBS via Heather Keller's RBS characterization scheme. These parts are cloned bluntly, without generating biobricks mixed sites in between.
discontinued: false
dominant: true
group u list: _11_
m user id: 0
owner id: 571
owning group id: 10
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: This part was generated by PCR assembly in order to minimize the number of digestions and ligations necessary to generate a larger construct. Especially given the short sequence of the three individual parts (and the difficulting in purifying small parts) it was much simpler to amplify the product in this way rather than generate the 3 parts individually and clone them together.
star: false
creator: Heather Keller
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
sequence annotation: AvrII site
sequence annotation: -35
sequence annotation: ss2
sequence annotation: ss1
sequence annotation: -10
sequence annotation: BBa_B0048
sequence annotation: hp2
sequence annotation: lac O1
sequence annotation: BBa_R0011
sequence annotation: BBa_B0100
sequence annotation: hp1
sequence: https://synbiohub.org/public/igem/BBa_S00159_sequence/1

AvrII site
type: SequenceAnnotation
persistent identity: annotation1920205
display id: annotation1920205
version: 1
top level: BBa_S00159
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_S00159/annotation1920205/range1920205/1

https://synbiohub.org/public/igem/BBa_S00159/annotation1920205/range1920205/1
type: Range
persistent identity: range1920205
display id: range1920205
version: 1
top level: BBa_S00159
direction: direction/forward
start: 171
end: 176
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1920197
display id: annotation1920197
version: 1
top level: BBa_S00159
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_S00159/annotation1920197/range1920197/1

https://synbiohub.org/public/igem/BBa_S00159/annotation1920197/range1920197/1
type: Range
persistent identity: range1920197
display id: range1920197
version: 1
top level: BBa_S00159
direction: direction/forward
start: 20
end: 25
orientation: inline

ss2
type: SequenceAnnotation
persistent identity: annotation1920203
display id: annotation1920203
version: 1
top level: BBa_S00159
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_S00159/annotation1920203/range1920203/1

https://synbiohub.org/public/igem/BBa_S00159/annotation1920203/range1920203/1
type: Range
persistent identity: range1920203
display id: range1920203
version: 1
top level: BBa_S00159
direction: direction/forward
start: 159
end: 170
orientation: inline

ss1
type: SequenceAnnotation
persistent identity: annotation1920201
display id: annotation1920201
version: 1
top level: BBa_S00159
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_S00159/annotation1920201/range1920201/1

https://synbiohub.org/public/igem/BBa_S00159/annotation1920201/range1920201/1
type: Range
persistent identity: range1920201
display id: range1920201
version: 1
top level: BBa_S00159
direction: direction/forward
start: 119
end: 129
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1920199
display id: annotation1920199
version: 1
top level: BBa_S00159
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_S00159/annotation1920199/range1920199/1

https://synbiohub.org/public/igem/BBa_S00159/annotation1920199/range1920199/1
type: Range
persistent identity: range1920199
display id: range1920199
version: 1
top level: BBa_S00159
direction: direction/forward
start: 43
end: 48
orientation: inline

BBa_B0048
type: SequenceAnnotation
persistent identity: annotation1920206
display id: annotation1920206
version: 1
top level: BBa_S00159
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_S00159/annotation1920206/range1920206/1

https://synbiohub.org/public/igem/BBa_S00159/annotation1920206/range1920206/1
type: Range
persistent identity: range1920206
display id: range1920206
version: 1
top level: BBa_S00159
direction: direction/reverse
start: 171
end: 176
orientation: inline

hp2
type: SequenceAnnotation
persistent identity: annotation1920202
display id: annotation1920202
version: 1
top level: BBa_S00159
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_S00159/annotation1920202/range1920202/1

https://synbiohub.org/public/igem/BBa_S00159/annotation1920202/range1920202/1
type: Range
persistent identity: range1920202
display id: range1920202
version: 1
top level: BBa_S00159
direction: direction/forward
start: 130
end: 158
orientation: inline

lac O1
type: SequenceAnnotation
persistent identity: annotation1920196
display id: annotation1920196
version: 1
top level: BBa_S00159
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_S00159/annotation1920196/range1920196/1

https://synbiohub.org/public/igem/BBa_S00159/annotation1920196/range1920196/1
type: Range
persistent identity: range1920196
display id: range1920196
version: 1
top level: BBa_S00159
direction: direction/forward
start: 3
end: 19
orientation: inline

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation1920195
display id: annotation1920195
version: 1
top level: BBa_S00159
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_S00159/annotation1920195/range1920195/1

https://synbiohub.org/public/igem/BBa_S00159/annotation1920195/range1920195/1
type: Range
persistent identity: range1920195
display id: range1920195
version: 1
top level: BBa_S00159
direction: direction/forward
start: 1
end: 54
orientation: inline

BBa_B0100
type: SequenceAnnotation
persistent identity: annotation1920204
display id: annotation1920204
version: 1
top level: BBa_S00159
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_S00159/annotation1920204/range1920204/1

https://synbiohub.org/public/igem/BBa_S00159/annotation1920204/range1920204/1
type: Range
persistent identity: range1920204
display id: range1920204
version: 1
top level: BBa_S00159
direction: direction/reverse
start: 56
end: 170
orientation: inline

lac O1
type: SequenceAnnotation
persistent identity: annotation1920198
display id: annotation1920198
version: 1
top level: BBa_S00159
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_S00159/annotation1920198/range1920198/1

https://synbiohub.org/public/igem/BBa_S00159/annotation1920198/range1920198/1
type: Range
persistent identity: range1920198
display id: range1920198
version: 1
top level: BBa_S00159
direction: direction/forward
start: 26
end: 42
orientation: inline

hp1
type: SequenceAnnotation
persistent identity: annotation1920200
display id: annotation1920200
version: 1
top level: BBa_S00159
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_S00159/annotation1920200/range1920200/1

https://synbiohub.org/public/igem/BBa_S00159/annotation1920200/range1920200/1
type: Range
persistent identity: range1920200
display id: range1920200
version: 1
top level: BBa_S00159
direction: direction/forward
start: 56
end: 118
orientation: inline

BBa_K113011
type: ComponentDefinition
persistent identity: BBa K113011
display id: BBa_K113011
version: 1
was generated by: iGEM to SBOL conversion
description: more overlapping T7 promoter
created: 2008-10-25T11:00:00Z
modified: 2015-05-08T01:09:20Z
mutable provenance: The sequence of T7 promoter is TAATACGACTCACTATA (from 5' to 3').
owned by: james
owned by: myers
mutable description: Two T7 promoters are reversely overlapping in five base pairs, with the third one mutated from A to G.
discontinued: false
dominant: false
experience: experience/None
group u list: _254_
m user id: 0
owner id: 2711
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: It is difficult to make this short DNA by PCR as both sides are T7 promoters, which are very similar. It is highly possible that they form loops before they can be synthesized by PCR. Thus we order two single stranded DNA and then anneal them together by touchdown PCR.
star: false
creator: Wu Jingjing, Wang Jinyu
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: T7 promoter
sequence: https://synbiohub.org/public/igem/BBa_K113011_sequence/1

T7 promoter
type: SequenceAnnotation
persistent identity: annotation1989380
display id: annotation1989380
version: 1
top level: BBa_K113011
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K113011/annotation1989380/range1989380/1

https://synbiohub.org/public/igem/BBa_K113011/annotation1989380/range1989380/1
type: Range
persistent identity: range1989380
display id: range1989380
version: 1
top level: BBa_K113011
direction: direction/forward
start: 17
end: 34
orientation: inline

T7 promoter
type: SequenceAnnotation
persistent identity: annotation1989359
display id: annotation1989359
version: 1
top level: BBa_K113011
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K113011/annotation1989359/range1989359/1

https://synbiohub.org/public/igem/BBa_K113011/annotation1989359/range1989359/1
type: Range
persistent identity: range1989359
display id: range1989359
version: 1
top level: BBa_K113011
direction: direction/reverse
start: 4
end: 21
orientation: inline

BBa_K921001
type: ComponentDefinition
persistent identity: BBa K921001
display id: BBa_K921001
version: 1
was generated by: iGEM to SBOL conversion
description: T7 RNAP + IPTG->PoPs (Mutant II)
created: 2012-09-30T11:00:00Z
modified: 2015-05-08T01:13:46Z
mutable provenance: The part consists of a sequences that resemble a T7 promoter and the lacO sequence found in E. coli.
owned by: james
owned by: myers
mutable description: This is a mutant T7 promoter that includes a lacO sequence. This is repressible by the LacI protein and is induced in the presence of IPTG. The promoter sits in the 5' region of the gene of interest and initiates transcription when the cell expresses T7 RNA polymerase. Be sure you are using a cell strain that includes this gene (DE3).
discontinued: false
dominant: false
experience: experience/Works
group u list: _1186_
m user id: 0
owner id: 12713
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: There are three portions to the T7 promoter: the recognition site, the melting box (or TATA box) and the initation site. Mutations in these sequences caused differential behavior from the wild type.
star: false
creator: Eric Pederson
type: DnaRegion
role: partType/T7
role: SO:0001207
sequence annotation: Initiation
sequence annotation: T7 Promoter
sequence annotation: LacO
sequence: https://synbiohub.org/public/igem/BBa_K921001_sequence/1

Initiation
type: SequenceAnnotation
persistent identity: annotation2205298
display id: annotation2205298
version: 1
top level: BBa_K921001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K921001/annotation2205298/range2205298/1

https://synbiohub.org/public/igem/BBa_K921001/annotation2205298/range2205298/1
type: Range
persistent identity: range2205298
display id: range2205298
version: 1
top level: BBa_K921001
direction: direction/forward
start: 18
end: 20
orientation: inline

T7 Promoter
type: SequenceAnnotation
persistent identity: annotation2205296
display id: annotation2205296
version: 1
top level: BBa_K921001
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K921001/annotation2205296/range2205296/1

https://synbiohub.org/public/igem/BBa_K921001/annotation2205296/range2205296/1
type: Range
persistent identity: range2205296
display id: range2205296
version: 1
top level: BBa_K921001
direction: direction/forward
start: 1
end: 20
orientation: inline

LacO
type: SequenceAnnotation
persistent identity: annotation2205297
display id: annotation2205297
version: 1
top level: BBa_K921001
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K921001/annotation2205297/range2205297/1

https://synbiohub.org/public/igem/BBa_K921001/annotation2205297/range2205297/1
type: Range
persistent identity: range2205297
display id: range2205297
version: 1
top level: BBa_K921001
direction: direction/forward
start: 22
end: 48
orientation: inline

BBa_R0182
type: ComponentDefinition
persistent identity: BBa R0182
display id: BBa_R0182
version: 1
was generated by: iGEM to SBOL conversion
description: T7 RNAP promoter
created: 2005-09-25T11:00:00Z
modified: 2015-05-08T01:14:15Z
owned by: james
owned by: myers
mutable description: T7 promoter with an A->T mutation at the -10 position of the consensus sequence
discontinued: false
dominant: false
experience: experience/None
group u list: _11_6_
m user id: 0
owner id: 135
owning group id: 6
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Barry Canton
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_R0182_sequence/1

BBa_K1614000
type: ComponentDefinition
persistent identity: BBa K1614000
display id: BBa_K1614000
version: 1
was generated by: iGEM to SBOL conversion
description: T7 promoter for expression of functional RNA
created: 2015-09-13T11:00:00Z
modified: 2015-09-14T12:29:29Z
mutable provenance: T7 phage minimal promoter (consensus)
owned by: james
owned by: myers
mutable description: Test
discontinued: false
dominant: false
experience: experience/None
group u list: _2031_
m user id: 12380
owner id: 16422
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: None.
star: false
creator: Stefan Holderbach
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: T7 promoter
sequence: https://synbiohub.org/public/igem/BBa_K1614000_sequence/1

T7 promoter
type: SequenceAnnotation
persistent identity: annotation2454728
display id: annotation2454728
version: 1
top level: BBa_K1614000
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1614000/annotation2454728/range2454728/1

https://synbiohub.org/public/igem/BBa_K1614000/annotation2454728/range2454728/1
type: Range
persistent identity: range2454728
display id: range2454728
version: 1
top level: BBa_K1614000
direction: direction/forward
start: 1
end: 17
orientation: inline

BBa_Z0252
type: ComponentDefinition
persistent identity: BBa Z0252
display id: BBa_Z0252
version: 1
was generated by: iGEM to SBOL conversion
description: T7 weak binding and processivity
created: 2005-04-04T11:00:00Z
modified: 2015-05-08T01:14:55Z
mutable provenance: T7
owned by: james
owned by: myers
mutable description: Promoter for T7 RNA polymerase
discontinued: false
dominant: false
experience: experience/None
group u list: _10_
m user id: 0
owner id: 250
owning group id: 10
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: T7.2
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: 23bp conserved region
sequence annotation: t -> a mutation from consensus
sequence annotation: tga -> acg mutation from consensus
sequence: https://synbiohub.org/public/igem/BBa_Z0252_sequence/1

23bp conserved region
type: SequenceAnnotation
persistent identity: annotation1476343
display id: annotation1476343
version: 1
top level: BBa_Z0252
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_Z0252/annotation1476343/range1476343/1

https://synbiohub.org/public/igem/BBa_Z0252/annotation1476343/range1476343/1
type: Range
persistent identity: range1476343
display id: range1476343
version: 1
top level: BBa_Z0252
direction: direction/forward
start: 6
end: 28
orientation: inline

t -> a mutation from consensus
type: SequenceAnnotation
persistent identity: annotation1476345
display id: annotation1476345
version: 1
top level: BBa_Z0252
role: feature/mutation
role: SO:0001059
location: https://synbiohub.org/public/igem/BBa_Z0252/annotation1476345/range1476345/1

https://synbiohub.org/public/igem/BBa_Z0252/annotation1476345/range1476345/1
type: Range
persistent identity: range1476345
display id: range1476345
version: 1
top level: BBa_Z0252
direction: direction/forward
start: 21
end: 21
orientation: inline

tga -> acg mutation from consensus
type: SequenceAnnotation
persistent identity: annotation1476344
display id: annotation1476344
version: 1
top level: BBa_Z0252
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_Z0252/annotation1476344/range1476344/1

https://synbiohub.org/public/igem/BBa_Z0252/annotation1476344/range1476344/1
type: Range
persistent identity: range1476344
display id: range1476344
version: 1
top level: BBa_Z0252
direction: direction/forward
start: 10
end: 12
orientation: inline

pT7
type: ComponentDefinition
persistent identity: BBa I719005
display id: BBa_I719005
version: 1
was generated by: iGEM to SBOL conversion
description: T7 Promoter
created: 2007-10-23T11:00:00Z
modified: 2015-08-31T04:07:53Z
mutable provenance: ---
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Just a T7 Promoter
discontinued: false
dominant: false
experience: experience/Works
group u list: _128_
m user id: 0
owner id: 2097
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable notes: None
star: true
creator: Imperial 2007
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I719005_sequence/1

BBa_J34814
type: ComponentDefinition
persistent identity: BBa J34814
display id: BBa_J34814
version: 1
was generated by: iGEM to SBOL conversion
description: T7 Promoter
created: 2006-10-25T11:00:00Z
modified: 2015-08-31T04:08:47Z
mutable provenance: a
owned by: james
owned by: myers
mutable description: to be used qith t7 polymerase BBa_J34811
discontinued: false
dominant: false
experience: experience/None
group u list: _62_
m user id: 0
owner id: 1085
owning group id: 62
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: a
star: false
creator: Alexandra Choutko
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J34814_sequence/1

BBa_Z0251
type: ComponentDefinition
persistent identity: BBa Z0251
display id: BBa_Z0251
version: 1
was generated by: iGEM to SBOL conversion
description: T7 strong promoter
created: 2005-04-04T11:00:00Z
modified: 2015-05-08T01:14:55Z
mutable provenance: bacteriophage T7
owned by: james
owned by: myers
mutable description: Promoter for T7 RNA polymerase
discontinued: false
dominant: false
experience: experience/None
group u list: _10_
m user id: 0
owner id: 250
owning group id: 10
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: T7.2
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: consensus sequence
sequence: https://synbiohub.org/public/igem/BBa_Z0251_sequence/1

consensus sequence
type: SequenceAnnotation
persistent identity: annotation1476333
display id: annotation1476333
version: 1
top level: BBa_Z0251
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_Z0251/annotation1476333/range1476333/1

https://synbiohub.org/public/igem/BBa_Z0251/annotation1476333/range1476333/1
type: Range
persistent identity: range1476333
display id: range1476333
version: 1
top level: BBa_Z0251
direction: direction/forward
start: 6
end: 28
orientation: inline

BBa_S03652
type: ComponentDefinition
persistent identity: BBa S03652
display id: BBa_S03652
version: 1
was generated by: iGEM to SBOL conversion
description: E0041:S03650
created: 2007-03-05T12:00:00Z
modified: 2015-05-08T01:14:26Z
owned by: james
owned by: myers
discontinued: false
dominant: false
experience: experience/None
group u list: _10_
m user id: 0
owner id: 571
owning group id: 10
sample status: In stock
status: status/Available
bookmark: false
star: false
creator: Heather Keller
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_E0041
component: BBa_B0012
component: BBa_B0010
component: BBa_B0101
sequence annotation: BBa_B0012
sequence annotation: BBa_E0041
sequence annotation: BBa_B0101
sequence annotation: BBa_B0010
sequence: https://synbiohub.org/public/igem/BBa_S03652_sequence/1

BBa_E0041
type: Component
persistent identity: component1920512
display id: component1920512
version: 1
top level: BBa_S03652
access: public
definition: BBa_E0041

BBa_B0012
type: Component
persistent identity: component1920517
display id: component1920517
version: 1
top level: BBa_S03652
access: public
definition: BBa_B0012

BBa_B0010
type: Component
persistent identity: component1920515
display id: component1920515
version: 1
top level: BBa_S03652
access: public
definition: BBa_B0010

BBa_B0101
type: Component
persistent identity: component1920514
display id: component1920514
version: 1
top level: BBa_S03652
access: public
definition: HP3 3

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1920517
display id: annotation1920517
version: 1
top level: BBa_S03652
location: https://synbiohub.org/public/igem/BBa_S03652/annotation1920517/range1920517/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_S03652/annotation1920517/range1920517/1
type: Range
persistent identity: range1920517
display id: range1920517
version: 1
top level: BBa_S03652
direction: direction/forward
start: 891
end: 931
orientation: inline

BBa_E0041
type: SequenceAnnotation
persistent identity: annotation1920512
display id: annotation1920512
version: 1
top level: BBa_S03652
location: https://synbiohub.org/public/igem/BBa_S03652/annotation1920512/range1920512/1
component: BBa_E0041

https://synbiohub.org/public/igem/BBa_S03652/annotation1920512/range1920512/1
type: Range
persistent identity: range1920512
display id: range1920512
version: 1
top level: BBa_S03652
direction: direction/reverse
start: 1
end: 728
orientation: inline

BBa_B0101
type: SequenceAnnotation
persistent identity: annotation1920514
display id: annotation1920514
version: 1
top level: BBa_S03652
location: https://synbiohub.org/public/igem/BBa_S03652/annotation1920514/range1920514/1
component: BBa_B0101

https://synbiohub.org/public/igem/BBa_S03652/annotation1920514/range1920514/1
type: Range
persistent identity: range1920514
display id: range1920514
version: 1
top level: BBa_S03652
direction: direction/reverse
start: 737
end: 794
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1920515
display id: annotation1920515
version: 1
top level: BBa_S03652
location: https://synbiohub.org/public/igem/BBa_S03652/annotation1920515/range1920515/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_S03652/annotation1920515/range1920515/1
type: Range
persistent identity: range1920515
display id: range1920515
version: 1
top level: BBa_S03652
direction: direction/forward
start: 803
end: 882
orientation: inline

BBa_K2084001
type: ComponentDefinition
persistent identity: BBa K2084001
display id: BBa_K2084001
version: 1
was generated by: iGEM to SBOL conversion
description: T3 Unit
created: 2016-10-11T11:00:00Z
modified: 2016-10-29T09:20:38Z
mutable provenance: PCR amplified T3 from BBa_K346000
owned by: james
owned by: myers
mutable description: This sequence encodes for the T3 RNAP.
discontinued: false
dominant: false
group u list: _2552_
m user id: 32066
owner id: 32131
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: Had to create primers to amplify just the T3
star: false
creator: Maya Lemmon-Kishi
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
sequence: https://synbiohub.org/public/igem/BBa_K2084001_sequence/1

BBa_K112000
type: ComponentDefinition
persistent identity: BBa K112000
display id: BBa_K112000
version: 1
was generated by: iGEM to SBOL conversion
description: T4 holin, complete CDS, berkeley standard
created: 2008-09-02T11:00:00Z
modified: 2015-05-08T01:09:13Z
mutable provenance: PCR from existing part.
owned by: james
owned by: myers
mutable description: T4 holin gene, with start and stop codons. This part is actually in BBb format.
discontinued: false
dominant: false
experience: experience/None
group u list: _224_
m user id: 0
owner id: 2998
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Bing Xia
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K112000_sequence/1

BBa_K1479005
type: ComponentDefinition
persistent identity: BBa K1479005
display id: BBa_K1479005
version: 1
was generated by: iGEM to SBOL conversion
created: 2014-10-05T11:00:00Z
modified: 2015-05-08T01:10:40Z
mutable provenance: NULL
owned by: james
owned by: myers
mutable description: This part is just for upload test. It is meaningless
discontinued: false
dominant: false
experience: experience/None
group u list: _1859_
m user id: 0
owner id: 21515
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: NULL
star: false
type: DnaRegion
role: partType/Promoter
sequence annotation: cds
sequence annotation: 1
sequence annotation: st
sequence: https://synbiohub.org/public/igem/BBa_K1479005_sequence/1

cds
type: SequenceAnnotation
persistent identity: annotation2417782
display id: annotation2417782
version: 1
top level: BBa_K1479005
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1479005/annotation2417782/range2417782/1

https://synbiohub.org/public/igem/BBa_K1479005/annotation2417782/range2417782/1
type: Range
persistent identity: range2417782
display id: range2417782
version: 1
top level: BBa_K1479005
direction: direction/forward
start: 3
end: 5
orientation: inline

1
type: SequenceAnnotation
persistent identity: annotation2417781
display id: annotation2417781
version: 1
top level: BBa_K1479005
role: feature/start
role: SO:0000318
location: https://synbiohub.org/public/igem/BBa_K1479005/annotation2417781/range2417781/1

https://synbiohub.org/public/igem/BBa_K1479005/annotation2417781/range2417781/1
type: Range
persistent identity: range2417781
display id: range2417781
version: 1
top level: BBa_K1479005
direction: direction/forward
start: 1
end: 2
orientation: inline

st
type: SequenceAnnotation
persistent identity: annotation2417783
display id: annotation2417783
version: 1
top level: BBa_K1479005
role: feature/stop
role: SO:0000319
location: https://synbiohub.org/public/igem/BBa_K1479005/annotation2417783/range2417783/1

https://synbiohub.org/public/igem/BBa_K1479005/annotation2417783/range2417783/1
type: Range
persistent identity: range2417783
display id: range2417783
version: 1
top level: BBa_K1479005
direction: direction/reverse
start: 6
end: 7
orientation: inline

BBa_R0085
type: ComponentDefinition
persistent identity: BBa R0085
display id: BBa_R0085
version: 1
was generated by: iGEM to SBOL conversion
description: T7 Consensus Promoter Sequence
created: 2005-02-21T12:00:00Z
modified: 2015-05-08T01:14:15Z
mutable provenance: Sequence obtained from Sri Kosuri
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: The T7 promoter should only produce PoPS when the T7 polymerase is also being expressed.
discontinued: false
dominant: false
experience: experience/None
group u list: _11_6_
m user id: 0
owner id: 135
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: false
creator: Barry Canton
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: Polymerase Binding Region
sequence annotation: Initiation Region
sequence annotation: Transcription Start Site
sequence: https://synbiohub.org/public/igem/BBa_R0085_sequence/1

Polymerase Binding Region
type: SequenceAnnotation
persistent identity: annotation1721521
display id: annotation1721521
version: 1
top level: BBa_R0085
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0085/annotation1721521/range1721521/1

https://synbiohub.org/public/igem/BBa_R0085/annotation1721521/range1721521/1
type: Range
persistent identity: range1721521
display id: range1721521
version: 1
top level: BBa_R0085
direction: direction/forward
start: 1
end: 11
orientation: inline

Initiation Region
type: SequenceAnnotation
persistent identity: annotation1721522
display id: annotation1721522
version: 1
top level: BBa_R0085
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_R0085/annotation1721522/range1721522/1

https://synbiohub.org/public/igem/BBa_R0085/annotation1721522/range1721522/1
type: Range
persistent identity: range1721522
display id: range1721522
version: 1
top level: BBa_R0085
direction: direction/forward
start: 12
end: 23
orientation: inline

Transcription Start Site
type: SequenceAnnotation
persistent identity: annotation1721520
display id: annotation1721520
version: 1
top level: BBa_R0085
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_R0085/annotation1721520/range1721520/1

https://synbiohub.org/public/igem/BBa_R0085/annotation1721520/range1721520/1
type: Range
persistent identity: range1721520
display id: range1721520
version: 1
top level: BBa_R0085
direction: direction/forward
start: 18
end: 18
orientation: inline

BBa_S03650
type: ComponentDefinition
persistent identity: BBa S03650
display id: BBa_S03650
version: 1
was generated by: iGEM to SBOL conversion
description: B0100:B0015
created: 2007-03-05T12:00:00Z
modified: 2015-05-08T01:14:26Z
owned by: james
owned by: myers
part status: Released HQ 2013
discontinued: false
dominant: false
experience: experience/None
group u list: _10_
m user id: 0
owner id: 571
owning group id: 10
sample status: In stock
status: status/Available
bookmark: false
star: false
creator: Heather Keller
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_B0012
component: BBa_B0101
component: BBa_B0010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0101
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_S03650_sequence/1

BBa_B0012
type: Component
persistent identity: component1920491
display id: component1920491
version: 1
top level: BBa_S03650
access: public
definition: BBa_B0012

BBa_B0101
type: Component
persistent identity: component1920488
display id: component1920488
version: 1
top level: BBa_S03650
access: public
definition: HP3 3

BBa_B0010
type: Component
persistent identity: component1920489
display id: component1920489
version: 1
top level: BBa_S03650
access: public
definition: BBa_B0010

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1920489
display id: annotation1920489
version: 1
top level: BBa_S03650
location: https://synbiohub.org/public/igem/BBa_S03650/annotation1920489/range1920489/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_S03650/annotation1920489/range1920489/1
type: Range
persistent identity: range1920489
display id: range1920489
version: 1
top level: BBa_S03650
direction: direction/forward
start: 67
end: 146
orientation: inline

BBa_B0101
type: SequenceAnnotation
persistent identity: annotation1920488
display id: annotation1920488
version: 1
top level: BBa_S03650
location: https://synbiohub.org/public/igem/BBa_S03650/annotation1920488/range1920488/1
component: BBa_B0101

https://synbiohub.org/public/igem/BBa_S03650/annotation1920488/range1920488/1
type: Range
persistent identity: range1920488
display id: range1920488
version: 1
top level: BBa_S03650
direction: direction/reverse
start: 1
end: 58
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1920491
display id: annotation1920491
version: 1
top level: BBa_S03650
location: https://synbiohub.org/public/igem/BBa_S03650/annotation1920491/range1920491/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_S03650/annotation1920491/range1920491/1
type: Range
persistent identity: range1920491
display id: range1920491
version: 1
top level: BBa_S03650
direction: direction/forward
start: 155
end: 195
orientation: inline

BBa_J64997
type: ComponentDefinition
persistent identity: BBa J64997
display id: BBa_J64997
version: 1
was generated by: iGEM to SBOL conversion
description: T7 consensus -10 and rest
created: 2007-03-25T11:00:00Z
modified: 2015-05-08T01:08:18Z
owned by: james
owned by: myers
discontinued: false
dominant: false
experience: experience/None
group u list: _98_
m user id: 0
owner id: 1428
owning group id: 98
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Sai Duriseti
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: T7-10
sequence: https://synbiohub.org/public/igem/BBa_J64997_sequence/1

T7-10
type: SequenceAnnotation
persistent identity: annotation1921746
display id: annotation1921746
version: 1
top level: BBa_J64997
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_J64997/annotation1921746/range1921746/1

https://synbiohub.org/public/igem/BBa_J64997/annotation1921746/range1921746/1
type: Range
persistent identity: range1921746
display id: range1921746
version: 1
top level: BBa_J64997
direction: direction/forward
start: 1
end: 19
orientation: inline

BBa_I712074
type: ComponentDefinition
persistent identity: BBa I712074
display id: BBa_I712074
version: 1
was generated by: iGEM to SBOL conversion
description: T7 promoter (strong promoter from T7 bacteriophage)
created: 2007-10-21T11:00:00Z
modified: 2015-08-31T04:07:46Z
mutable provenance: T7 bacteriophage
owned by: james
owned by: myers
mutable description: T7 promoter is very specific promoter which is transcribed only by specific T7 RNA polymerase. Usually this promoter is used in expression systems where T7 promoter is cotransfected with T7 RNA polymerase. That ensures strong transcription of desired genes.
discontinued: false
dominant: false
experience: experience/Works
group u list: _130_
m user id: 0
owner id: 1856
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Rok Gaber
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I712074_sequence/1

ccdB
type: ComponentDefinition
persistent identity: BBa P1010
display id: BBa_P1010
version: 1
was generated by: iGEM to SBOL conversion
description: ccdB cell death gene
created: 2004-07-27T11:00:00Z
modified: 2015-05-08T01:14:11Z
owned by: james
owned by: myers
part status: Deleted
mutable description: The ccd operon having a constitutive promoter and the ccdB coding region. ccdB protein is lethal in normal cloning strains. This part is used to aid the process of moving a BioBrick part to a new plasmid. A target plasmid carrying P1010 is cut and mixed with the cut insert. Plasmids that recombine are selected against because they kill the cell that picks them up. P1010 is only provided as plasmid or in DB3.1 which is ccdB resistant.
discontinued: true
dominant: true
experience: experience/Works
group u list: _11_1_
m user id: 0
owner id: 60
owning group id: 7
sample status: Discontinued
status: status/Deleted
bookmark: false
star: false
creator: Leon Chan
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: T to C mutation
sequence annotation: -10 (rev)
sequence annotation: -35 (rev)
sequence annotation: ccdB (rev)
sequence annotation: ccdA- (rev)
sequence: https://synbiohub.org/public/igem/BBa_P1010_sequence/1

T to C mutation
type: SequenceAnnotation
persistent identity: annotation1747334
display id: annotation1747334
version: 1
top level: ccdB
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_P1010/annotation1747334/range1747334/1

https://synbiohub.org/public/igem/BBa_P1010/annotation1747334/range1747334/1
type: Range
persistent identity: range1747334
display id: range1747334
version: 1
top level: ccdB
direction: direction/forward
start: 467
end: 467
orientation: inline

-10 (rev)
type: SequenceAnnotation
persistent identity: annotation1747335
display id: annotation1747335
version: 1
top level: ccdB
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_P1010/annotation1747335/range1747335/1

https://synbiohub.org/public/igem/BBa_P1010/annotation1747335/range1747335/1
type: Range
persistent identity: range1747335
display id: range1747335
version: 1
top level: ccdB
direction: direction/reverse
start: 595
end: 600
orientation: inline

-35 (rev)
type: SequenceAnnotation
persistent identity: annotation1747336
display id: annotation1747336
version: 1
top level: ccdB
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_P1010/annotation1747336/range1747336/1

https://synbiohub.org/public/igem/BBa_P1010/annotation1747336/range1747336/1
type: Range
persistent identity: range1747336
display id: range1747336
version: 1
top level: ccdB
direction: direction/reverse
start: 618
end: 623
orientation: inline

ccdB (rev)
type: SequenceAnnotation
persistent identity: annotation1747332
display id: annotation1747332
version: 1
top level: ccdB
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_P1010/annotation1747332/range1747332/1

https://synbiohub.org/public/igem/BBa_P1010/annotation1747332/range1747332/1
type: Range
persistent identity: range1747332
display id: range1747332
version: 1
top level: ccdB
direction: direction/reverse
start: 29
end: 334
orientation: inline

ccdA- (rev)
type: SequenceAnnotation
persistent identity: annotation1747333
display id: annotation1747333
version: 1
top level: ccdB
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_P1010/annotation1747333/range1747333/1

https://synbiohub.org/public/igem/BBa_P1010/annotation1747333/range1747333/1
type: Range
persistent identity: range1747333
display id: range1747333
version: 1
top level: ccdB
direction: direction/reverse
start: 336
end: 556
orientation: inline

BBa_K1833999
type: ComponentDefinition
persistent identity: BBa K1833999
display id: BBa_K1833999
version: 1
was generated by: iGEM to SBOL conversion
description: T7 promoter as annealed oligos
created: 2015-09-14T11:00:00Z
modified: 2015-09-16T07:58:33Z
mutable provenance: Synthesized oligonucleotides as described on main page.
owned by: james
owned by: myers
mutable description: This is a part used as a building block for T7 driven expression. However, this part uses a special method with the following synthesized nucleotides: pT7sense: aattcgcggccgcttctagataatacgactcactatagggagaa pT7antisense: ctagttctccctatagtgagtcgtattatctagaagcggccgcg The following method can be used to insert a T7 promoter before a desired Biobrick part: 1. Cut the desired plasmid (which should contain an RBS and cds at minimum) with EcoRI and XbaI. Purify the plasmid with a method of your choice (both gel purification and PCR purification kits from Qiagen serve to remove the short undesired oligonucleotide from the digestion). 2. Be sure that your oligos have 5' phosphorylation (treat unphosphorylated oligos with T4 Polynucleotide Kinase). Then, anneal the two oligos by slowly cooling from 98C to room temperature. 3. Finally, ligate the cut plasmid with the annealed oligos by using a 1:10 molar ratio of plasmid to insert. 4. Transform the ligation reaction into E. coli, and plate. 5. Pick colonies, and verify the success of the cloning with sequencing or PCR. (Doing a diagnostic digest is not recommended as the size of the original plasmid and the desired plasmid are very similar.) 6. Add a composite part to the iGEM registry by using this part with the original protein coding part.
discontinued: false
dominant: false
experience: experience/None
group u list: _2259_
m user id: 27388
owner id: 27388
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: When ligating with a cut plasmid, this leaves a Biobrick scar between the T7 promoter and original Biobrick part.
star: false
creator: Konstantin Borisov
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: pT7
sequence annotation: Oligo scar
sequence: https://synbiohub.org/public/igem/BBa_K1833999_sequence/1

pT7
type: SequenceAnnotation
persistent identity: annotation2460074
display id: annotation2460074
version: 1
top level: BBa_K1833999
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1833999/annotation2460074/range2460074/1

https://synbiohub.org/public/igem/BBa_K1833999/annotation2460074/range2460074/1
type: Range
persistent identity: range2460074
display id: range2460074
version: 1
top level: BBa_K1833999
direction: direction/forward
start: 1
end: 23
orientation: inline

Oligo scar
type: SequenceAnnotation
persistent identity: annotation2460116
display id: annotation2460116
version: 1
top level: BBa_K1833999
role: feature/scar
location: https://synbiohub.org/public/igem/BBa_K1833999/annotation2460116/range2460116/1

https://synbiohub.org/public/igem/BBa_K1833999/annotation2460116/range2460116/1
type: Range
persistent identity: range2460116
display id: range2460116
version: 1
top level: BBa_K1833999
direction: direction/forward
start: 24
end: 29
orientation: inline

BBa_R0181
type: ComponentDefinition
persistent identity: BBa R0181
display id: BBa_R0181
version: 1
was generated by: iGEM to SBOL conversion
description: T7 RNAP promoter
created: 2005-09-25T11:00:00Z
modified: 2015-05-08T01:14:15Z
owned by: james
owned by: myers
mutable description: T7 promoter with an T->G mutation at the -17 position of the consensus sequence
discontinued: false
dominant: false
experience: experience/None
group u list: _11_6_
m user id: 0
owner id: 135
owning group id: 6
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Barry Canton
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_R0181_sequence/1

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

BBa_K113010
type: ComponentDefinition
persistent identity: BBa K113010
display id: BBa_K113010
version: 1
was generated by: iGEM to SBOL conversion
description: overlapping T7 promoter
created: 2008-10-25T11:00:00Z
modified: 2015-05-08T01:09:20Z
mutable provenance: The sequence of T7 promoter is TAATACGACTCACTATA.
owned by: james
owned by: myers
mutable description: Two T7 promoters are reversely overlapping.
discontinued: false
dominant: false
experience: experience/None
group u list: _254_
m user id: 0
owner id: 2711
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: It is difficult to make this short DNA by PCR as both sides are T7 promoters, which are very similar. It is highly possible that they form loops before they can be synthesized by PCR. Thus we order two single stranded DNA and then anneal them together by touchdown PCR.
star: false
creator: Wu Jingjing, Wang Jinyu
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: T7 promoter
sequence: https://synbiohub.org/public/igem/BBa_K113010_sequence/1

T7 promoter
type: SequenceAnnotation
persistent identity: annotation1989277
display id: annotation1989277
version: 1
top level: BBa_K113010
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K113010/annotation1989277/range1989277/1

https://synbiohub.org/public/igem/BBa_K113010/annotation1989277/range1989277/1
type: Range
persistent identity: range1989277
display id: range1989277
version: 1
top level: BBa_K113010
direction: direction/reverse
start: 4
end: 21
orientation: inline

T7 promoter
type: SequenceAnnotation
persistent identity: annotation1989307
display id: annotation1989307
version: 1
top level: BBa_K113010
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K113010/annotation1989307/range1989307/1

https://synbiohub.org/public/igem/BBa_K113010/annotation1989307/range1989307/1
type: Range
persistent identity: range1989307
display id: range1989307
version: 1
top level: BBa_K113010
direction: direction/forward
start: 20
end: 37
orientation: inline

BBa_K346000
type: ComponentDefinition
persistent identity: BBa K346000
display id: BBa_K346000
version: 1
was generated by: iGEM to SBOL conversion
description: RBS(B0032)+T3 DNA-directed RNA polymerase
created: 2010-09-24T11:00:00Z
modified: 2015-05-08T01:12:08Z
mutable provenance: Tn21
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: This part is the coding region of MerR, which is a mercury responsive regulator from Tn21.
discontinued: false
dominant: false
experience: experience/Works
group u list: _457_
m user id: 0
owner id: 4411
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable notes: We cloned this part from plasmid NR1, which was a gift from Anne O. Summers.
star: false
creator: Haoqian Zhang and Weiye Wang
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
sequence annotation: b0032
sequence annotation: t3 pol
sequence: https://synbiohub.org/public/igem/BBa_K346000_sequence/1

b0032
type: SequenceAnnotation
persistent identity: annotation2092860
display id: annotation2092860
version: 1
top level: BBa_K346000
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K346000/annotation2092860/range2092860/1

https://synbiohub.org/public/igem/BBa_K346000/annotation2092860/range2092860/1
type: Range
persistent identity: range2092860
display id: range2092860
version: 1
top level: BBa_K346000
direction: direction/forward
start: 1
end: 14
orientation: inline

t3 pol
type: SequenceAnnotation
persistent identity: annotation2092859
display id: annotation2092859
version: 1
top level: BBa_K346000
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K346000/annotation2092859/range2092859/1

https://synbiohub.org/public/igem/BBa_K346000/annotation2092859/range2092859/1
type: Range
persistent identity: range2092859
display id: range2092859
version: 1
top level: BBa_K346000
direction: direction/forward
start: 19
end: 2674
orientation: inline

T7
type: ComponentDefinition
persistent identity: BBa K145014
display id: BBa_K145014
version: 1
was generated by: iGEM to SBOL conversion
description: T7 RNA Polymerase with UmuD derived tag
created: 2008-07-23T11:00:00Z
modified: 2015-05-08T01:10:28Z
mutable provenance: Part composed of the T7 RNA Polymerase and the N-terminal degradation tag, but with no scar (no stop codon)
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: T7 RNA Polymerase with N-terminal UmuD degradation tag.
discontinued: false
dominant: false
experience: experience/None
group u list: _257_
m user id: 0
owner id: 2939
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable notes: This part is different from the simple composition of the tag and the polymerase, as there cannot be a stop codon in the scar.
star: true
creator: Benjamien Moeyaert
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: start
sequence annotation: stop
sequence annotation: tag
sequence annotation: cds
sequence: https://synbiohub.org/public/igem/BBa_K145014_sequence/1

start
type: SequenceAnnotation
persistent identity: annotation1968238
display id: annotation1968238
version: 1
top level: T7
role: feature/start
role: SO:0000318
location: https://synbiohub.org/public/igem/BBa_K145014/annotation1968238/range1968238/1

https://synbiohub.org/public/igem/BBa_K145014/annotation1968238/range1968238/1
type: Range
persistent identity: range1968238
display id: range1968238
version: 1
top level: T7
direction: direction/forward
start: 1
end: 3
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1968239
display id: annotation1968239
version: 1
top level: T7
role: feature/stop
role: SO:0000319
location: https://synbiohub.org/public/igem/BBa_K145014/annotation1968239/range1968239/1

https://synbiohub.org/public/igem/BBa_K145014/annotation1968239/range1968239/1
type: Range
persistent identity: range1968239
display id: range1968239
version: 1
top level: T7
direction: direction/forward
start: 2740
end: 2742
orientation: inline

tag
type: SequenceAnnotation
persistent identity: annotation1968241
display id: annotation1968241
version: 1
top level: T7
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K145014/annotation1968241/range1968241/1

https://synbiohub.org/public/igem/BBa_K145014/annotation1968241/range1968241/1
type: Range
persistent identity: range1968241
display id: range1968241
version: 1
top level: T7
direction: direction/forward
start: 1
end: 87
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1968240
display id: annotation1968240
version: 1
top level: T7
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K145014/annotation1968240/range1968240/1

https://synbiohub.org/public/igem/BBa_K145014/annotation1968240/range1968240/1
type: Range
persistent identity: range1968240
display id: range1968240
version: 1
top level: T7
direction: direction/forward
start: 2737
end: 2739
orientation: inline

cds
type: SequenceAnnotation
persistent identity: annotation1968242
display id: annotation1968242
version: 1
top level: T7
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K145014/annotation1968242/range1968242/1

https://synbiohub.org/public/igem/BBa_K145014/annotation1968242/range1968242/1
type: Range
persistent identity: range1968242
display id: range1968242
version: 1
top level: T7
direction: direction/forward
start: 88
end: 2736
orientation: inline

BBa_R0185
type: ComponentDefinition
persistent identity: BBa R0185
display id: BBa_R0185
version: 1
was generated by: iGEM to SBOL conversion
description: T7 promoter (lacI repressible)
created: 2008-04-07T11:00:00Z
modified: 2015-05-08T01:14:15Z
mutable provenance: This sequence is based on the T7lac promoter described by Dubendorff and Studier (JMB 1991, 219, 45-59). The only modification is the mutation at -10.
owned by: james
owned by: myers
mutable description: A lac repressible T7 promoter. This promoter is similar to those found in the pET vectors with T7lac promoters. I mutated the -10 base to a G, which should make it about 4% of the strength of consensus. The base numbering is as used by Studier and coworkers (JMB 1991, 219, 45-59). See BBa_R0183 for more information.
discontinued: false
dominant: false
experience: experience/None
group u list: _11_
m user id: 0
owner id: 135
owning group id: 84
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: The -10 mutation from consensus was included to weaken the consensus promoter as described by Imburgio and co-workers (see BBa_R0183 for more information)
star: false
creator: Bartholomew Canton
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: LacI binding site
sequence annotation: T7 promoter sequence
sequence: https://synbiohub.org/public/igem/BBa_R0185_sequence/1

LacI binding site
type: SequenceAnnotation
persistent identity: annotation1962093
display id: annotation1962093
version: 1
top level: BBa_R0185
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0185/annotation1962093/range1962093/1

https://synbiohub.org/public/igem/BBa_R0185/annotation1962093/range1962093/1
type: Range
persistent identity: range1962093
display id: range1962093
version: 1
top level: BBa_R0185
direction: direction/forward
start: 20
end: 44
orientation: inline

T7 promoter sequence
type: SequenceAnnotation
persistent identity: annotation1962092
display id: annotation1962092
version: 1
top level: BBa_R0185
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0185/annotation1962092/range1962092/1

https://synbiohub.org/public/igem/BBa_R0185/annotation1962092/range1962092/1
type: Range
persistent identity: range1962092
display id: range1962092
version: 1
top level: BBa_R0185
direction: direction/forward
start: 1
end: 20
orientation: inline

BBa_R0187
type: ComponentDefinition
persistent identity: BBa R0187
display id: BBa_R0187
version: 1
was generated by: iGEM to SBOL conversion
description: T7 promoter (lacI repressible)
created: 2008-04-07T11:00:00Z
modified: 2015-05-08T01:14:15Z
mutable provenance: This sequence is based on the T7lac promoter described by Dubendorff and Studier (JMB 1991, 219, 45-59). The only modification is the mutation at -9.
owned by: james
owned by: myers
mutable description: A lac repressible T7 promoter. This promoter is similar to those found in the pET vectors with T7lac promoters. I mutated the -9 base to an G, which should make it less than 3% of the strength of consensus. The base numbering is as used by Studier and coworkers (JMB 1991, 219, 45-59). See BBa_R0183 for more information.
discontinued: false
dominant: false
experience: experience/None
group u list: _11_
m user id: 0
owner id: 135
owning group id: 84
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: The -9 mutation from consensus was included to weaken the consensus promoter as described by Imburgio and co-workers (see BBa_R0183 for more information)
star: false
creator: Bartholomew Canton
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: LacI binding site
sequence annotation: T7 promoter
sequence: https://synbiohub.org/public/igem/BBa_R0187_sequence/1

LacI binding site
type: SequenceAnnotation
persistent identity: annotation1962096
display id: annotation1962096
version: 1
top level: BBa_R0187
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0187/annotation1962096/range1962096/1

https://synbiohub.org/public/igem/BBa_R0187/annotation1962096/range1962096/1
type: Range
persistent identity: range1962096
display id: range1962096
version: 1
top level: BBa_R0187
direction: direction/forward
start: 20
end: 44
orientation: inline

T7 promoter
type: SequenceAnnotation
persistent identity: annotation1962097
display id: annotation1962097
version: 1
top level: BBa_R0187
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0187/annotation1962097/range1962097/1

https://synbiohub.org/public/igem/BBa_R0187/annotation1962097/range1962097/1
type: Range
persistent identity: range1962097
display id: range1962097
version: 1
top level: BBa_R0187
direction: direction/forward
start: 1
end: 20
orientation: inline

https://synbiohub.org/public/igem/BBa_R0185_sequence/1
type: Sequence
persistent identity: BBa R0185 sequence
display id: BBa_R0185_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: taatacggctcactataggggaattgtgagcggataacaattcc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_Z0251_sequence/1
type: Sequence
persistent identity: BBa Z0251 sequence
display id: BBa_Z0251_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gaaattaatacgactcactatagggagaccacaac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_S03653_sequence/1
type: Sequence
persistent identity: BBa S03653 sequence
display id: BBa_S03653_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gctcttctatggtgagcaagggcgaggaggataacatggccatcatcaaggagttcatgcgcttcaaggtgcacatggagggctccgtgaacggccacgagttcgagatcgagggcgagggcgagggccgcccctacgagggcacccagaccgccaagctgaaggtgaccaagggtggccccctgcccttcgcctgggacatcctgtcccctcagttcatgtacggctccaaggcctacgtgaagcaccccgccgacatccccgactacttgaagctgtccttccccgagggcttcaagtgggagcgcgtgatgaacttcgaggacggcggcgtggtgaccgtgacccaggactcctccttgcaggacggcgagttcatctacaaggtgaagctgcgcggcaccaacttcccctccgacggccccgtaatgcagaagaagaccatgggctgggaggcctcctccgagcggatgtaccccgaggacggcgccctgaagggcgagatcaagcagaggctgaagctgaaggacggcggccactacgacgctgaggtcaagaccacctacaaggccaagaagcccgtgcagctgcccggcgcctacaacgtcaacatcaagttggacatcacctcccacaacgaggactacaccatcgtggaacagtacgaacgcgccgagggccgccactccaccggcggcatggacgagctgtacaagtaataatactagaggatcgcctgatcccggtgcacccgggcagctgcatagtctgggtgcaccgggatcaggtactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1833009_sequence/1
type: Sequence
persistent identity: BBa K1833009 sequence
display id: BBa_K1833009_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: taatacgactcactatagggaaagaggagaaaatgagtatcagctcgcgtgtgaaaagtaaacgcatccagctgggactgaatcaggccgagctggcgcagaaagttggtacgagtaacgtgtctatttcccagctggaacgcggaaaaaccaaacgcccgcgtttcctgcctgaactggcaagcgccttgggcgtgtctgtagactggcttttaaacggcacttctgattcgaatgttcgctttgtcggccatgtggagccgaaaggcaaatacccactgattagcatggtacgcgccggtagctggtgctgataaaaccccttggggcctctaaacgggtcttgaggggttttttg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E0041_sequence/1
type: Sequence
persistent identity: BBa E0041 sequence
display id: BBa_E0041_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gctcttctatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_G00113_sequence/1
type: Sequence
persistent identity: BBa G00113 sequence
display id: BBa_G00113_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: taatacgactcactataggg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K113012_sequence/1
type: Sequence
persistent identity: BBa K113012 sequence
display id: BBa_K113012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cccctatagagagtcgtattaatacgactctctatagggg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I719005_sequence/1
type: Sequence
persistent identity: BBa I719005 sequence
display id: BBa_I719005_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: taatacgactcactatagggaga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K093001_sequence/1
type: Sequence
persistent identity: BBa K093001 sequence
display id: BBa_K093001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggcacttcttgaccttaaacaattctatgagttacgtgaaggctgcgacgacaagggtatccttgtgatggacggcgactggctggtcttccaagctatgagtgctgctgagtttgatgcctcttgggaggaagagatttggcaccgatgctgtgaccacgctaaggcccgtcagattcttgaggattccattaagtcctacgagacccgtaagaaggcttgggcaggtgctccaattgtccttgcgttcaccgatagtgttaactggcgtaaagaactggttgacccgaactataaggctaaccgtaaggccgtgaagaaacctgtagggtactttgagttccttgatgctctctttgagcgcgaagagttctattgcatccgtgagcctatgcttgagggtgatgacgttatgggagttattgcttccaatccgtctgccttcggtgctcgtaaggctgtaatcatctcttgcgataaggactttaagaccatccctaactgtgacttcctgtggtgtaccactggtaacatcctgactcagaccgaagagtccgctgactggtggcacctcttccagaccatcaagggtgacatcactgatggttactcagggattgctggatggggtgataccgccgaggacttcttgaataacccgttcataaccgagcctaaaacgtctgtgcttaagtccggtaagaacaaaggccaagaggttactaaatgggttaaacgcgaccctgagcctcatgagacgctttgggactgcattaagtccattggcgcgaaggctggtatgaccgaagaggatattatcaagcagggccaaatggctcgaatcctacggttcaacgagtacaactttattgacaaggagatttacctgtggagaccgtaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0180_sequence/1
type: Sequence
persistent identity: BBa R0180 sequence
display id: BBa_R0180_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttatacgactcactatagggaga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_P1010_sequence/1
type: Sequence
persistent identity: BBa P1010 sequence
display id: BBa_P1010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: actggctgtgtataagggagcctgacatttatattccccagaacatcaggttaatggcgtttttgatgtcattttcgcggtggctgagatcagccacttcttccccgataacggagaccggcacactggccatatcggtggtcatcatgcgccagctttcatccccgatatgcaccaccgggtaaagttcacgggagactttatctgacagcagacgtgcactggccagggggatcaccatccgtcgcccgggcgtgtcaataatatcactctgtacatccacaaacagacgataacggctctctcttttataggtgtaaaccttaaactgcatttcaccagcccctgttctcgtcagcaaaagagccgttcatttcaataaaccgggcgacctcagccatcccttcctgattttccgctttccagcgttcggcacgcagacgacgggcttcattctgcatggttgtgcttaccagaccggagatattgacatcatatatgccttgagcaactgatagctgtcgctgtcaactgtcactgtaatacgctgcttcatagcatacctctttttgacatacttcgggtatacatatcagtatatattcttataccgcaaaaatcagcgcgcaaatacgcatactgttatctggcttttagtaagccggatccacgcgt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1833002_sequence/1
type: Sequence
persistent identity: BBa K1833002 sequence
display id: BBa_K1833002_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aattaaccctcactaaagggagaactagaattaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J64997_sequence/1
type: Sequence
persistent identity: BBa J64997 sequence
display id: BBa_J64997_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: taatacgactcactatagg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K145014_sequence/1
type: Sequence
persistent identity: BBa K145014 sequence
display id: BBa_K145014_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgttgtttatcaagcctgcggatctccgcgaaattgtgacttttccgctatttagcgatcttgttcagtgtggctttccttcaccgatgaacacgattaacatcgctaagaacgacttctctgacatcgaactggctgctatcccgttcaacactctggctgaccattacggtgagcgtttagctcgcgaacagttggcccttgagcatgagtcttacgagatgggtgaagcacgcttccgcaagatgtttgagcgtcaacttaaagctggtgaggttgcggataacgctgccgccaagcctctcatcactaccctactccctaagatgattgcacgcatcaacgactggtttgaggaagtgaaagctaagcgcggcaagcgcccgacagccttccagttcctgcaagaaatcaagccggaagccgtagcgtacatcaccattaagaccactctggcttgcctaaccagtgctgacaatacaaccgttcaggctgtagcaagcgcaatcggtcgggccattgaggacgaggctcgcttcggtcgtatccgtgaccttgaagctaagcacttcaagaaaaacgttgaggaacaactcaacaagcgcgtagggcacgtctacaagaaagcatttatgcaagttgtcgaggctgacatgctctctaagggtctactcggtggcgaggcgtggtcttcgtggcataaggaagactctattcatgtaggagtacgctgcatcgagatgctcattgagtcaaccggaatggttagcttacaccgccaaaatgctggcgtagtaggtcaagactctgagactatcgaactcgcacctgaatacgctgaggctatcgcaacccgtgcaggtgcgctggctggcatctctccgatgttccaaccttgcgtagttcctcctaagccgtggactggcattactggtggtggctattgggctaacggtcgtcgtcctctggcgctggtgcgtactcacagtaagaaagcactgatgcgctacgaagacgtttacatgcctgaggtgtacaaagcgattaacattgcgcaaaacaccgcatggaaaatcaacaagaaagtcctagcggtcgccaacgtaatcaccaagtggaagcattgtccggtcgaggacatccctgcgattgagcgtgaagaactcccgatgaaaccggaagacatcgacatgaatcctgaggctctcaccgcgtggaaacgtgctgccgctgctgtgtaccgcaaggacaaggctcgcaagtctcgccgtatcagccttgagttcatgcttgagcaagccaataagtttgctaaccataaggccatctggttcccttacaacatggactggcgcggtcgtgtttacgctgtgtcaatgttcaacccgcaaggtaacgatatgaccaaaggactgcttacgctggcgaaaggtaaaccaatcggtaaggaaggttactactggctgaaaatccacggtgcaaactgtgcgggtgtcgataaggttccgttccctgagcgcatcaagttcattgaggaaaaccacgagaacatcatggcttgcgctaagtctccactggagaacacttggtgggctgagcaagattctccgttctgcttccttgcgttctgctttgagtacgctggggtacagcaccacggcctgagctataactgctcccttccgctggcgtttgacgggtcttgctctggcatccagcacttctccgcgatgctccgagatgaggtaggtggtcgcgcggttaacttgcttcctagtgaaaccgttcaggacatctacgggattgttgctaagaaagtcaacgagattctacaagcagacgcaatcaatgggaccgataacgaagtagttaccgtgaccgatgagaacactggtgaaatctctgagaaagtcaagctgggcactaaggcactggctggtcaatggctggcttacggtgttactcgcagtgtgactaagcgttcagtcatgacgctggc…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_S03651_sequence/1
type: Sequence
persistent identity: BBa S03651 sequence
display id: BBa_S03651_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacagccaggggtgctcggcataagccgaagatatcggtagagttaatattgagcagatcccccggtgaaggatttaaccgtgttatctcgttggagatattcatggcgtattttggatcctaggtactagagactggctgtgtataagggagcctgacatttatattccccagaacatcaggttaatggcgtttttgatgtcattttcgcggtggctgagatcagccacttcttccccgataacggagaccggcacactggccatatcggtggtcatcatgcgccagctttcatccccgatatgcaccaccgggtaaagttcacgggagactttatctgacagcagacgtgcactggccagggggatcaccatccgtcgcccgggcgtgtcaataatatcactctgtacatccacaaacagacgataacggctctctcttttataggtgtaaaccttaaactgcatttcaccagcccctgttctcgtcagcaaaagagccgttcatttcaataaaccgggcgacctcagccatcccttcctgattttccgctttccagcgttcggcacgcagacgacgggcttcattctgcatggttgtgcttaccagaccggagatattgacatcatatatgccttgagcaactgatagctgtcgctgtcaactgtcactgtaatacgctgcttcatagcatacctctttttgacatacttcgggtatacatatcagtatatattcttataccgcaaaaatcagcgcgcaaatacgcatactgttatctggcttttagtaagccggatccacgcgt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K921001_sequence/1
type: Sequence
persistent identity: BBa K921001 sequence
display id: BBa_K921001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: taatacgactcactacagggcggaattgtgagcggataacaattccaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J34814_sequence/1
type: Sequence
persistent identity: BBa J34814 sequence
display id: BBa_J34814_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gaatttaatacgactcactatagggaga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_Z0252_sequence/1
type: Sequence
persistent identity: BBa Z0252 sequence
display id: BBa_Z0252_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ataattaattgaactcactaaagggagaccacagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K2084000_sequence/1
type: Sequence
persistent identity: BBa K2084000 sequence
display id: BBa_K2084000_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aattaaccctcactaaagggaga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K731700_sequence/1
type: Sequence
persistent identity: BBa K731700 sequence
display id: BBa_K731700_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tactagtagcggccgctgcagaaggagaataatctatgagcaaaggcgaagaactgttcacgggtgtggttccgatcctggttgaactggatggcgatgtgaacggtcataaatttagcgtgtctggtgaaggcgaaggtgatgcgacctacggcaaactgacgctgaaactgatttgcaccacgggtaaactgccggttccgtggccgaccctggtgaccacgctgggttatggtctgatgtgtttcgcacgttacccggatcacatgaaacgccatgatttctttaaatctgcgatgccggaaggctatgtgcaggaacgtaccatctttttcaaagatgatggtaactacaaaacccgcgcggaagttaaatttgaaggcgatacgctggtgaaccgtattgaactgaaaggtatcgatttcaaagaagatggcaatattctgggtcacaaactggaatacaactacaacagtcataacgtgtacattaccgccgataaacagaaaaacggtatcaaagcaaacttcaaaatccgtcacaacatcgaagatggcggtgttcagctggccgatcattaccagcagaacaccccgattggcgatggtccggtgctgctgccggataatcattatctgagttaccagagcaaactgtctaaagatccgaatgaaaaacgcgatcacatggttctgctggaatttgtgaccgcggccggcattacgcatggtatggatgaactgtataaataactcgagcaccaccaccaccaccactgagatccggctgctaacaaagcccgaaaggaagctgagttggctgctgccaccgctgagcaataactagcataaccccttggggcctctaaacgggtcttgaggggttttttgctgaaaggaggaactatatccggatggcgaatgggacgcgccctgtagcggcgcattaagcgcggcgggtgtggtggttacgcgcagcgtgaccgctacacttgccagcgccctagcgcccgctcctttcgctttcttcccttcctttctcgccacgttcgccggctttccccgtcaagctctaaatcgggggctccctttagggttccgatttagtgctttacggcacctcgaccccaaaaaacttgattagggtgatggttcacgtagtgggccatcgccctgatagacggtttttcgccctttgacgttggagtccacgttctttaatagtggactcttgttccaaactggaacaacactcaaccctatctcggtctattcttttgatttataagggattttgccgatttcggcctattggttaaaaaatgagctgatttaacaaaaatttaacgcgaattttaacaaaatattaacgtttacaatttcaggtggcacttttcggggaaatgtgcgcggaacccctatttgtttatttttctaaatacattcaaatatgtatccgctcatgagacaataaccctgataaatgcttcaataatattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattcccttttttgcggcattttgccttcctgtttttgctcacccagaaacgctggtgaaagtaaaagatgctgaagatcagttgggtgcacgagtgggttacatcgaactggatctcaacagcggtaagatccttgagagttttcgccccgaagaacgttttccaatgatgagcacttttaaagttctgctatgtggcgcggtattatcccgtattgacgccgggcaagagcaactcggtcgccgcatacactattctcagaatgacttggttgagtactcaccagtcacagaaaagcatcttacggatggcatgacagtaagagaattatgcagtgctgccataaccatgagtgataacactgcggccaacttacttctgacaacgatcggaggaccgaaggagctaaccgcttttttgcacaacatgggggatcatgtaactcgccttgatcgttg…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1833000_sequence/1
type: Sequence
persistent identity: BBa K1833000 sequence
display id: BBa_K1833000_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: taatacgactcactatagggagaactagaattaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1479005_sequence/1
type: Sequence
persistent identity: BBa K1479005 sequence
display id: BBa_K1479005_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aattccggaattccgg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0085_sequence/1
type: Sequence
persistent identity: BBa R0085 sequence
display id: BBa_R0085_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: taatacgactcactatagggaga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0181_sequence/1
type: Sequence
persistent identity: BBa R0181 sequence
display id: BBa_R0181_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gaatacgactcactatagggaga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0183_sequence/1
type: Sequence
persistent identity: BBa R0183 sequence
display id: BBa_R0183_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcatacgactcactatagggaga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1833999_sequence/1
type: Sequence
persistent identity: BBa K1833999 sequence
display id: BBa_K1833999_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: taatacgactcactatagggagaactaga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1833997_sequence/1
type: Sequence
persistent identity: BBa K1833997 sequence
display id: BBa_K1833997_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aattaaccctcactaaagggagaactaga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K346000_sequence/1
type: Sequence
persistent identity: BBa K346000 sequence
display id: BBa_K346000_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacacaggaaagtactagatgaacatcatcgaaaacatcgaaaagaatgacttctcagaaatcgaactggctgctatcccgttcaacacactggctgaccactacggaagcgccttggctaaagagcagttggctttagaacatgagtcttatgagctaggcgagcgccgcttcctcaagatgcttgagcgtcaagcgaaagctggtgagattgcagacaacgcagccgctaagccgttactcgctacgcttctccctaagttaaccacacgtatcgtcgagtggctcgaagagtacgcatcgaagaaaggccgcaagcctagcgcatacgcaccgctccagttactcaagccggaggcctccgcgtttatcaccctgaaagttatccttgcgtcactaaccagtacgaacatgacaaccattcaggccgctgctggtatgctggggaaagccattgaggacgaggcacgatttgggcgcatccgtgacctagaagcgaagcacttcaagaagcacgttgaggaacagcttaacaagcgccacgggcaagtctacaagaaagcatttatgcaggtggtcgaggccgatatgattggtcgaggtctgcttggtggcgaggcgtggtctagctgggataaagaaaccacgatgcacgtagggattcgcctgattgaaatgctgattgaatccacgggtctggtggaattacagcgccacaacgcaggtaacgcaggctctgaccatgaggcactgcaactggcccaagagtacgtggacgtattagcgaagcgtgcaggcgctctggcgggtatctctccgatgttccagccgtgtgtcgtaccgccgaaaccttgggtagcaatcacagggggcggctattgggctaacggtcgcagacctttggcactcgttcgcactcactctaagaagggcttgatgcgctacgaagacgtttacatgccagaagtctacaaggctgtgaacctcgcgcaaaacaccgcatggaaaatcaacaagaaagttcttgctgttgtcaatgagattgttaactggaagaattgcccggtagcagacattccatcgctggagcgccaagagttaccgcctaagcctgacgacattgacaccaacgaggcagcgctcaaggagtggaagaaagccgctgctggtatctatcgcttggacaaggcacgagtgtctcgccgtatcagcttagagttcatgctggagcaggccaacaagttcgcaagtaagaaagcaatctggttcccttacaacatggactggcgcggtcgtgtgtacgctgtgccgatgttcaacccgcaaggcaacgacatgacgaaaggtctgctgacccttgctaaaggcaagccaatcggtgaggaaggtttctactggctgaaaatccacggtgcgaactgtgcgggtgttgataaggttccattcccggagcgcatcgcgttcattgagaagcacgtagacgacattctggcttgcgctaaagacccaatcaataacacttggtgggctgagcaggattcaccgttctgtttcctcgcgttttgcttcgagtatgcaggcgttacgcaccacggtctgagctacaattgctctctgccgctggcgttcgacgggtcttgctctggtatccagcacttctccgcgatgctccgcgatgaggtaggcggtcgtgcggttaacctgctgccaagcgaaaccgtgcaggacatttacggcatcgttgcacagaaagtaaacgagattctcaaacaggatgcaatcaacggcacgcctaacgagatgattaccgtgaccgacaaggacaccggggaaatctcagagaagctcaaacttggaacctcaacgctggcgcaacagtggctggcatatggtgtaacccgtagcgtaactaaacgttcggtcatgacgctggcttacggttccaaggagttcggctttcgtcaacaggtattggatgacaccattcagcctgcaattg…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0186_sequence/1
type: Sequence
persistent identity: BBa R0186 sequence
display id: BBa_R0186_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: taatacgaatcactataggggaattgtgagcggataacaattcc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K921000_sequence/1
type: Sequence
persistent identity: BBa K921000 sequence
display id: BBa_K921000_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: taatgcgactcactataggacaattgtgggcggacaacaattccaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K2084004_sequence/1
type: Sequence
persistent identity: BBa K2084004 sequence
display id: BBa_K2084004_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aattaaccctcactaaagggagatactagagaaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_S03650_sequence/1
type: Sequence
persistent identity: BBa S03650 sequence
display id: BBa_S03650_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gatcgcctgatcccggtgcacccgggcagctgcatagtctgggtgcaccgggatcaggtactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0182_sequence/1
type: Sequence
persistent identity: BBa R0182 sequence
display id: BBa_R0182_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: taatacgtctcactatagggaga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K921002_sequence/1
type: Sequence
persistent identity: BBa K921002 sequence
display id: BBa_K921002_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: caatccgactcactaaagagagaattgtgagcggataacaattccaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E0042_sequence/1
type: Sequence
persistent identity: BBa E0042 sequence
display id: BBa_E0042_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gctcttctatggtgagcaagggcgaggaggataacatggccatcatcaaggagttcatgcgcttcaaggtgcacatggagggctccgtgaacggccacgagttcgagatcgagggcgagggcgagggccgcccctacgagggcacccagaccgccaagctgaaggtgaccaagggtggccccctgcccttcgcctgggacatcctgtcccctcagttcatgtacggctccaaggcctacgtgaagcaccccgccgacatccccgactacttgaagctgtccttccccgagggcttcaagtgggagcgcgtgatgaacttcgaggacggcggcgtggtgaccgtgacccaggactcctccttgcaggacggcgagttcatctacaaggtgaagctgcgcggcaccaacttcccctccgacggccccgtaatgcagaagaagaccatgggctgggaggcctcctccgagcggatgtaccccgaggacggcgccctgaagggcgagatcaagcagaggctgaagctgaaggacggcggccactacgacgctgaggtcaagaccacctacaaggccaagaagcccgtgcagctgcccggcgcctacaacgtcaacatcaagttggacatcacctcccacaacgaggactacaccatcgtggaacagtacgaacgcgccgagggccgccactccaccggcggcatggacgagctgtacaagtaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I712074_sequence/1
type: Sequence
persistent identity: BBa I712074 sequence
display id: BBa_I712074_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: taatacgactcactatagggaatacaagctacttgttctttttgca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E0040_sequence/1
type: Sequence
persistent identity: BBa E0040 sequence
display id: BBa_E0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0187_sequence/1
type: Sequence
persistent identity: BBa R0187 sequence
display id: BBa_R0187_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: taatacgagtcactataggggaattgtgagcggataacaattcc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K2084001_sequence/1
type: Sequence
persistent identity: BBa K2084001 sequence
display id: BBa_K2084001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaacatcatcgaaaacatcgaaaagaatgacttctcagaaatcgaactggctgctatcccgttcaacacactggctgaccactacggaagcgccttggctaaagagcagttggctttagaacatgagtcttatgagctaggcgagcgccgcttcctcaagatgcttgagcgtcaagcgaaagctggtgagattgcagacaacgcagccgctaagccgttactcgctacgcttctccctaagttaaccacacgtatcgtcgagtggctcgaagagtacgcatcgaagaaaggccgcaagcctagcgcatacgcaccgctccagttactcaagccggaggcctccgcgtttatcaccctgaaagttatccttgcgtcactaaccagtacgaacatgacaaccattcaggccgctgctggtatgctggggaaagccattgaggacgaggcacgatttgggcgcatccgtgacctagaagcgaagcacttcaagaagcacgttgaggaacagcttaacaagcgccacgggcaagtctacaagaaagcatttatgcaggtggtcgaggccgatatgattggtcgaggtctgcttggtggcgaggcgtggtctagctgggataaagaaaccacgatgcacgtagggattcgcctgattgaaatgctgattgaatccacgggtctggtggaattacagcgccacaacgcaggtaacgcaggctctgaccatgaggcactgcaactggcccaagagtacgtggacgtattagcgaagcgtgcaggcgctctggcgggtatctctccgatgttccagccgtgtgtcgtaccgccgaaaccttgggtagcaatcacagggggcggctattgggctaacggtcgcagacctttggcactcgttcgcactcactctaagaagggcttgatgcgctacgaagacgtttacatgccagaagtctacaaggctgtgaacctcgcgcaaaacaccgcatggaaaatcaacaagaaagttcttgctgttgtcaatgagattgttaactggaagaattgcccggtagcagacattccatcgctggagcgccaagagttaccgcctaagcctgacgacattgacaccaacgaggcagcgctcaaggagtggaagaaagccgctgctggtatctatcgcttggacaaggcacgagtgtctcgccgtatcagcttagagttcatgctggagcaggccaacaagttcgcaagtaagaaagcaatctggttcccttacaacatggactggcgcggtcgtgtgtacgctgtgccgatgttcaacccgcaaggcaacgacatgacgaaaggtctgctgacccttgctaaaggcaagccaatcggtgaggaaggtttctactggctgaaaatccacggtgcgaactgtgcgggtgttgataaggttccattcccggagcgcatcgcgttcattgagaagcacgtagacgacattctggcttgcgctaaagacccaatcaataacacttggtgggctgagcaggattcaccgttctgtttcctcgcgttttgcttcgagtatgcaggcgttacgcaccacggtctgagctacaattgctctctgccgctggcgttcgacgggtcttgctctggtatccagcacttctccgcgatgctccgcgatgaggtaggcggtcgtgcggttaacctgctgccaagcgaaaccgtgcaggacatttacggcatcgttgcacagaaagtaaacgagattctcaaacaggatgcaatcaacggcacgcctaacgagatgattaccgtgaccgacaaggacaccggggaaatctcagagaagctcaaacttggaacctcaacgctggcgcaacagtggctggcatatggtgtaacccgtagcgtaactaaacgttcggtcatgacgctggcttacggttccaaggagttcggctttcgtcaacaggtattggatgacaccattcagcctgcaattgacagcggtaagggcttgat…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_S03652_sequence/1
type: Sequence
persistent identity: BBa S03652 sequence
display id: BBa_S03652_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gctcttctatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagaggatcgcctgatcccggtgcacccgggcagctgcatagtctgggtgcaccgggatcaggtactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0184_sequence/1
type: Sequence
persistent identity: BBa R0184 sequence
display id: BBa_R0184_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcatacgactcactataggggaattgtgagcggataacaattcc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K112000_sequence/1
type: Sequence
persistent identity: BBa K112000 sequence
display id: BBa_K112000_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggcagcacctagaatatcattttcgccctctgatattctatttggtgttctcgatcgcttgttcaaagataacgctaccgggaaggttcttgcttcccgggtagctgtcgtaattcttttgtttataatggcgattgtttggtataggggagatagtttctttgagtactataagcaatcaaagtatgaaacatacagtgaaattattgaaaaggaaagaactgcacgctttgaatctgtcgccctggaacaactccagatagttcatatatcatctgaggcagactttagtgcggtgtattctttccgccctaaaaacttaaactattttgttgatattatagcatacgaaggaaaattaccttcaacaataagtgaaaaatcacttggaggatatcctgttgataaaactatggatgaatatacagttcatttaaatggacgtcattattattccaactcaaaatttgcttttttaccaactaaaaagcctactcccgaaataaactacatgtacagttgtccatattttaatttggataatatctatgctggaacgataaccatgtactggtatagaaatgatcatataagtaatgaccgccttgaatcaatatgtgctcaggcggccagaatattaggaagggctaaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_Z0253_sequence/1
type: Sequence
persistent identity: BBa Z0253 sequence
display id: BBa_Z0253_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: agcaccgaagtaatacgactcactattagggaaga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0030_sequence/1
type: Sequence
persistent identity: BBa B0030 sequence
display id: BBa_B0030_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: attaaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1614000_sequence/1
type: Sequence
persistent identity: BBa K1614000 sequence
display id: BBa_K1614000_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: taatacgactcactatag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0101_sequence/1
type: Sequence
persistent identity: BBa B0101 sequence
display id: BBa_B0101_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gatcgcctgatcccggtgcacccgggcagctgcatagtctgggtgcaccgggatcagg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K113011_sequence/1
type: Sequence
persistent identity: BBa K113011 sequence
display id: BBa_K113011_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cccctatagtgagtcgtactacgactcactatagggg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E0840_sequence/1
type: Sequence
persistent identity: BBa E0840 sequence
display id: BBa_E0840_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: attaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K113010_sequence/1
type: Sequence
persistent identity: BBa K113010 sequence
display id: BBa_K113010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cccctatagtgagtcgtattaatacgactcactatagggg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_S00159_sequence/1
type: Sequence
persistent identity: BBa S00159 sequence
display id: BBa_S00159_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacagccaggggtgctcggcataagccgaagatatcggtagagttaatattgagcagatcccccggtgaaggatttaaccgtgttatctcgttggagatattcatggcgtattttggatcctagg
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z