//chassis/bacteriophage/t7
type: Collection
persistent identity: chassis bacteriophage t7
display id: chassis_bacteriophage_t7
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
owned by: james
owned by: myers
member: BBa_S03652
member: BBa_Z0253
member: BBa_K113011
member: BBa_K921001
member: BBa_K113010
member: BBa_J34814
member: BBa_R0186
member: BBa_R0182
member: T7
member: BBa_K113012
member: BBa_K1833000
member: BBa_K1833999
member: BBa_K921000
member: BBa_S03651
member: BBa_K1614000
member: BBa_R0187
member: BBa_Z0252
member: BBa_K1833009
member: BBa_R0183
member: BBa_J64997
member: BBa_I712074
member: BBa_S03650
member: pT7
member: BBa_Z0251
member: BBa_R0180
member: BBa_R0085
member: BBa_R0184
member: T7Gene6
member: BBa_K921002
member: BBa_S03653
member: BBa_R0181
member: BBa_K731700
member: BBa_R0185

BBa_S03651
type: ComponentDefinition
persistent identity: BBa S03651
display id: BBa_S03651
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: S00159:P1010
creator: Heather Keller
created: 2007-03-05T12:00:00Z
modified: 2015-06-15T12:38:52Z
discontinued: true
dominant: false
experience: experience/None
group u list: _10_
m user id: 4206
owner id: 571
owning group id: 10
sample status: It's complicated
status: status/Deleted
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_S00159
component: BBa_P1010
sequence annotation: BBa_P1010
sequence annotation: BBa_S00159
sequence: https://synbiohub.org/public/igem/BBa_S03651_sequence/1

BBa_S00159
type: Component
persistent identity: component1920355
display id: component1920355
version: 1
top level: BBa_S03651
access: public
definition: https://synbiohub.org/public/igem/BBa_S00159/1

BBa_P1010
type: Component
persistent identity: component1920361
display id: component1920361
version: 1
top level: BBa_S03651
access: public
definition: https://synbiohub.org/public/igem/BBa_P1010/1

BBa_P1010
type: SequenceAnnotation
persistent identity: annotation1920361
display id: annotation1920361
version: 1
top level: BBa_S03651
location: https://synbiohub.org/public/igem/BBa_S03651/annotation1920361/range1920361/1
component: BBa_P1010

https://synbiohub.org/public/igem/BBa_S03651/annotation1920361/range1920361/1
type: Range
persistent identity: range1920361
display id: range1920361
version: 1
direction: direction/reverse
top level: BBa_S03651
start: 185
end: 859
orientation: inline

BBa_S00159
type: SequenceAnnotation
persistent identity: annotation1920355
display id: annotation1920355
version: 1
top level: BBa_S03651
location: https://synbiohub.org/public/igem/BBa_S03651/annotation1920355/range1920355/1
component: BBa_S00159

https://synbiohub.org/public/igem/BBa_S03651/annotation1920355/range1920355/1
type: Range
persistent identity: range1920355
display id: range1920355
version: 1
direction: direction/reverse
top level: BBa_S03651
start: 1
end: 176
orientation: inline

BBa_R0180
type: ComponentDefinition
persistent identity: BBa R0180
display id: BBa_R0180
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: T7 RNAP promoter
creator: Barry Canton
created: 2005-09-25T11:00:00Z
modified: 2015-05-08T01:14:15Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_6_
m user id: 0
owner id: 135
owning group id: 6
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: T7 promoter with an A->T mutation at the -16 position of the consensus sequence
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_R0180_sequence/1

T7
type: ComponentDefinition
persistent identity: BBa G00113
display id: BBa_G00113
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: T7 promoter sequencing primer, 20-mer
creator: Reshma Shetty
created: 2008-10-16T11:00:00Z
modified: 2015-08-31T04:07:28Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _41_
m user id: 0
owner id: 126
owning group id: 162
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This primer anneals to the T7 Promoter region of any vector containing the T7 Promoter.
mutable notes: <biblio> #Wallace pmid=6282692 </biblio>
mutable provenance: T7 promoter.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000112
role: partType/Primer
sequence: https://synbiohub.org/public/igem/BBa_G00113_sequence/1

T7
type: ComponentDefinition
persistent identity: BBa K145014
display id: BBa_K145014
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: T7 RNA Polymerase with UmuD derived tag
creator: Benjamien Moeyaert
created: 2008-07-23T11:00:00Z
modified: 2015-05-08T01:10:28Z
discontinued: false
dominant: false
experience: experience/None
group u list: _257_
m user id: 0
owner id: 2939
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: T7 RNA Polymerase with N-terminal UmuD degradation tag.
mutable notes: This part is different from the simple composition of the tag and the polymerase, as there cannot be a stop codon in the scar.
mutable provenance: Part composed of the T7 RNA Polymerase and the N-terminal degradation tag, but with no scar (no stop codon)
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: start
sequence annotation: cds
sequence annotation: stop
sequence annotation: tag
sequence: https://synbiohub.org/public/igem/BBa_K145014_sequence/1

start
type: SequenceAnnotation
persistent identity: annotation1968238
display id: annotation1968238
version: 1
top level: T7
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K145014/annotation1968238/range1968238/1

https://synbiohub.org/public/igem/BBa_K145014/annotation1968238/range1968238/1
type: Range
persistent identity: range1968238
display id: range1968238
version: 1
direction: direction/forward
top level: T7
start: 1
end: 3
orientation: inline

cds
type: SequenceAnnotation
persistent identity: annotation1968242
display id: annotation1968242
version: 1
top level: T7
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K145014/annotation1968242/range1968242/1

https://synbiohub.org/public/igem/BBa_K145014/annotation1968242/range1968242/1
type: Range
persistent identity: range1968242
display id: range1968242
version: 1
direction: direction/forward
top level: T7
start: 88
end: 2736
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1968239
display id: annotation1968239
version: 1
top level: T7
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K145014/annotation1968239/range1968239/1

https://synbiohub.org/public/igem/BBa_K145014/annotation1968239/range1968239/1
type: Range
persistent identity: range1968239
display id: range1968239
version: 1
direction: direction/forward
top level: T7
start: 2740
end: 2742
orientation: inline

tag
type: SequenceAnnotation
persistent identity: annotation1968241
display id: annotation1968241
version: 1
top level: T7
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K145014/annotation1968241/range1968241/1

https://synbiohub.org/public/igem/BBa_K145014/annotation1968241/range1968241/1
type: Range
persistent identity: range1968241
display id: range1968241
version: 1
direction: direction/forward
top level: T7
start: 1
end: 87
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1968240
display id: annotation1968240
version: 1
top level: T7
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K145014/annotation1968240/range1968240/1

https://synbiohub.org/public/igem/BBa_K145014/annotation1968240/range1968240/1
type: Range
persistent identity: range1968240
display id: range1968240
version: 1
direction: direction/forward
top level: T7
start: 2737
end: 2739
orientation: inline

BBa_R0187
type: ComponentDefinition
persistent identity: BBa R0187
display id: BBa_R0187
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: T7 promoter (lacI repressible)
creator: Bartholomew Canton
created: 2008-04-07T11:00:00Z
modified: 2015-05-08T01:14:15Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_
m user id: 0
owner id: 135
owning group id: 84
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: A lac repressible T7 promoter. This promoter is similar to those found in the pET vectors with T7lac promoters. I mutated the -9 base to an G, which should make it less than 3% of the strength of consensus. The base numbering is as used by Studier and coworkers (JMB 1991, 219, 45-59). See BBa_R0183 for more information.
mutable notes: The -9 mutation from consensus was included to weaken the consensus promoter as described by Imburgio and co-workers (see BBa_R0183 for more information)
mutable provenance: This sequence is based on the T7lac promoter described by Dubendorff and Studier (JMB 1991, 219, 45-59). The only modification is the mutation at -9.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: LacI binding site
sequence annotation: T7 promoter
sequence: https://synbiohub.org/public/igem/BBa_R0187_sequence/1

LacI binding site
type: SequenceAnnotation
persistent identity: annotation1962096
display id: annotation1962096
version: 1
top level: BBa_R0187
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0187/annotation1962096/range1962096/1

https://synbiohub.org/public/igem/BBa_R0187/annotation1962096/range1962096/1
type: Range
persistent identity: range1962096
display id: range1962096
version: 1
direction: direction/forward
top level: BBa_R0187
start: 20
end: 44
orientation: inline

T7 promoter
type: SequenceAnnotation
persistent identity: annotation1962097
display id: annotation1962097
version: 1
top level: BBa_R0187
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0187/annotation1962097/range1962097/1

https://synbiohub.org/public/igem/BBa_R0187/annotation1962097/range1962097/1
type: Range
persistent identity: range1962097
display id: range1962097
version: 1
direction: direction/forward
top level: BBa_R0187
start: 1
end: 20
orientation: inline

BBa_Z0253
type: ComponentDefinition
persistent identity: BBa Z0253
display id: BBa_Z0253
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: T7 weak binding promoter
creator: Sriram Kosuri
created: 2006-05-15T11:00:00Z
modified: 2015-05-08T01:14:55Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_10_
m user id: 0
owner id: 64
owning group id: 10
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: ag->TA from concensus
sequence annotation: ag->GA from concensus
sequence: https://synbiohub.org/public/igem/BBa_Z0253_sequence/1

ag->TA from concensus
type: SequenceAnnotation
persistent identity: annotation1862684
display id: annotation1862684
version: 1
top level: BBa_Z0253
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_Z0253/annotation1862684/range1862684/1

https://synbiohub.org/public/igem/BBa_Z0253/annotation1862684/range1862684/1
type: Range
persistent identity: range1862684
display id: range1862684
version: 1
direction: direction/forward
top level: BBa_Z0253
start: 27
end: 28
orientation: inline

ag->GA from concensus
type: SequenceAnnotation
persistent identity: annotation1862691
display id: annotation1862691
version: 1
top level: BBa_Z0253
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_Z0253/annotation1862691/range1862691/1

https://synbiohub.org/public/igem/BBa_Z0253/annotation1862691/range1862691/1
type: Range
persistent identity: range1862691
display id: range1862691
version: 1
direction: direction/forward
top level: BBa_Z0253
start: 31
end: 32
orientation: inline

BBa_K113011
type: ComponentDefinition
persistent identity: BBa K113011
display id: BBa_K113011
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: more overlapping T7 promoter
creator: Wu Jingjing, Wang Jinyu
created: 2008-10-25T11:00:00Z
modified: 2015-05-08T01:09:20Z
discontinued: false
dominant: false
experience: experience/None
group u list: _254_
m user id: 0
owner id: 2711
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Two T7 promoters are reversely overlapping in five base pairs, with the third one mutated from A to G.
mutable notes: It is difficult to make this short DNA by PCR as both sides are T7 promoters, which are very similar. It is highly possible that they form loops before they can be synthesized by PCR. Thus we order two single stranded DNA and then anneal them together by touchdown PCR.
mutable provenance: The sequence of T7 promoter is TAATACGACTCACTATA (from 5' to 3').
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: T7 promoter
sequence: https://synbiohub.org/public/igem/BBa_K113011_sequence/1

T7 promoter
type: SequenceAnnotation
persistent identity: annotation1989359
display id: annotation1989359
version: 1
top level: BBa_K113011
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K113011/annotation1989359/range1989359/1

https://synbiohub.org/public/igem/BBa_K113011/annotation1989359/range1989359/1
type: Range
persistent identity: range1989359
display id: range1989359
version: 1
direction: direction/reverse
top level: BBa_K113011
start: 4
end: 21
orientation: inline

T7 promoter
type: SequenceAnnotation
persistent identity: annotation1989380
display id: annotation1989380
version: 1
top level: BBa_K113011
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K113011/annotation1989380/range1989380/1

https://synbiohub.org/public/igem/BBa_K113011/annotation1989380/range1989380/1
type: Range
persistent identity: range1989380
display id: range1989380
version: 1
direction: direction/forward
top level: BBa_K113011
start: 17
end: 34
orientation: inline

BBa_Z0252
type: ComponentDefinition
persistent identity: BBa Z0252
display id: BBa_Z0252
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: T7 weak binding and processivity
creator: T7.2
created: 2005-04-04T11:00:00Z
modified: 2015-05-08T01:14:55Z
discontinued: false
dominant: false
experience: experience/None
group u list: _10_
m user id: 0
owner id: 250
owning group id: 10
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Promoter for T7 RNA polymerase
mutable provenance: T7
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: 23bp conserved region
sequence annotation: t -> a mutation from consensus
sequence annotation: tga -> acg mutation from consensus
sequence: https://synbiohub.org/public/igem/BBa_Z0252_sequence/1

23bp conserved region
type: SequenceAnnotation
persistent identity: annotation1476343
display id: annotation1476343
version: 1
top level: BBa_Z0252
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_Z0252/annotation1476343/range1476343/1

https://synbiohub.org/public/igem/BBa_Z0252/annotation1476343/range1476343/1
type: Range
persistent identity: range1476343
display id: range1476343
version: 1
direction: direction/forward
top level: BBa_Z0252
start: 6
end: 28
orientation: inline

t -> a mutation from consensus
type: SequenceAnnotation
persistent identity: annotation1476345
display id: annotation1476345
version: 1
top level: BBa_Z0252
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_Z0252/annotation1476345/range1476345/1

https://synbiohub.org/public/igem/BBa_Z0252/annotation1476345/range1476345/1
type: Range
persistent identity: range1476345
display id: range1476345
version: 1
direction: direction/forward
top level: BBa_Z0252
start: 21
end: 21
orientation: inline

tga -> acg mutation from consensus
type: SequenceAnnotation
persistent identity: annotation1476344
display id: annotation1476344
version: 1
top level: BBa_Z0252
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_Z0252/annotation1476344/range1476344/1

https://synbiohub.org/public/igem/BBa_Z0252/annotation1476344/range1476344/1
type: Range
persistent identity: range1476344
display id: range1476344
version: 1
direction: direction/forward
top level: BBa_Z0252
start: 10
end: 12
orientation: inline

BBa_R0185
type: ComponentDefinition
persistent identity: BBa R0185
display id: BBa_R0185
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: T7 promoter (lacI repressible)
creator: Bartholomew Canton
created: 2008-04-07T11:00:00Z
modified: 2015-05-08T01:14:15Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_
m user id: 0
owner id: 135
owning group id: 84
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: A lac repressible T7 promoter. This promoter is similar to those found in the pET vectors with T7lac promoters. I mutated the -10 base to a G, which should make it about 4% of the strength of consensus. The base numbering is as used by Studier and coworkers (JMB 1991, 219, 45-59). See BBa_R0183 for more information.
mutable notes: The -10 mutation from consensus was included to weaken the consensus promoter as described by Imburgio and co-workers (see BBa_R0183 for more information)
mutable provenance: This sequence is based on the T7lac promoter described by Dubendorff and Studier (JMB 1991, 219, 45-59). The only modification is the mutation at -10.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: LacI binding site
sequence annotation: T7 promoter sequence
sequence: https://synbiohub.org/public/igem/BBa_R0185_sequence/1

LacI binding site
type: SequenceAnnotation
persistent identity: annotation1962093
display id: annotation1962093
version: 1
top level: BBa_R0185
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0185/annotation1962093/range1962093/1

https://synbiohub.org/public/igem/BBa_R0185/annotation1962093/range1962093/1
type: Range
persistent identity: range1962093
display id: range1962093
version: 1
direction: direction/forward
top level: BBa_R0185
start: 20
end: 44
orientation: inline

T7 promoter sequence
type: SequenceAnnotation
persistent identity: annotation1962092
display id: annotation1962092
version: 1
top level: BBa_R0185
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0185/annotation1962092/range1962092/1

https://synbiohub.org/public/igem/BBa_R0185/annotation1962092/range1962092/1
type: Range
persistent identity: range1962092
display id: range1962092
version: 1
direction: direction/forward
top level: BBa_R0185
start: 1
end: 20
orientation: inline

BBa_S03652
type: ComponentDefinition
persistent identity: BBa S03652
display id: BBa_S03652
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: E0041:S03650
creator: Heather Keller
created: 2007-03-05T12:00:00Z
modified: 2015-05-08T01:14:26Z
discontinued: false
dominant: false
experience: experience/None
group u list: _10_
m user id: 0
owner id: 571
owning group id: 10
sample status: In stock
status: status/Available
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_E0041
component: BBa_B0012
component: BBa_B0010
component: BBa_B0101
sequence annotation: BBa_B0010
sequence annotation: BBa_E0041
sequence annotation: BBa_B0012
sequence annotation: BBa_B0101
sequence: https://synbiohub.org/public/igem/BBa_S03652_sequence/1

BBa_E0041
type: Component
persistent identity: component1920512
display id: component1920512
version: 1
top level: BBa_S03652
access: public
definition: https://synbiohub.org/public/igem/BBa_E0041/1

BBa_B0012
type: Component
persistent identity: component1920517
display id: component1920517
version: 1
top level: BBa_S03652
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0010
type: Component
persistent identity: component1920515
display id: component1920515
version: 1
top level: BBa_S03652
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0101
type: Component
persistent identity: component1920514
display id: component1920514
version: 1
top level: BBa_S03652
access: public
definition: https://synbiohub.org/public/igem/BBa_B0101/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1920515
display id: annotation1920515
version: 1
top level: BBa_S03652
location: https://synbiohub.org/public/igem/BBa_S03652/annotation1920515/range1920515/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_S03652/annotation1920515/range1920515/1
type: Range
persistent identity: range1920515
display id: range1920515
version: 1
direction: direction/forward
top level: BBa_S03652
start: 803
end: 882
orientation: inline

BBa_E0041
type: SequenceAnnotation
persistent identity: annotation1920512
display id: annotation1920512
version: 1
top level: BBa_S03652
location: https://synbiohub.org/public/igem/BBa_S03652/annotation1920512/range1920512/1
component: BBa_E0041

https://synbiohub.org/public/igem/BBa_S03652/annotation1920512/range1920512/1
type: Range
persistent identity: range1920512
display id: range1920512
version: 1
direction: direction/reverse
top level: BBa_S03652
start: 1
end: 728
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1920517
display id: annotation1920517
version: 1
top level: BBa_S03652
location: https://synbiohub.org/public/igem/BBa_S03652/annotation1920517/range1920517/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_S03652/annotation1920517/range1920517/1
type: Range
persistent identity: range1920517
display id: range1920517
version: 1
direction: direction/forward
top level: BBa_S03652
start: 891
end: 931
orientation: inline

BBa_B0101
type: SequenceAnnotation
persistent identity: annotation1920514
display id: annotation1920514
version: 1
top level: BBa_S03652
location: https://synbiohub.org/public/igem/BBa_S03652/annotation1920514/range1920514/1
component: BBa_B0101

https://synbiohub.org/public/igem/BBa_S03652/annotation1920514/range1920514/1
type: Range
persistent identity: range1920514
display id: range1920514
version: 1
direction: direction/reverse
top level: BBa_S03652
start: 737
end: 794
orientation: inline

BBa_R0181
type: ComponentDefinition
persistent identity: BBa R0181
display id: BBa_R0181
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: T7 RNAP promoter
creator: Barry Canton
created: 2005-09-25T11:00:00Z
modified: 2015-05-08T01:14:15Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_6_
m user id: 0
owner id: 135
owning group id: 6
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: T7 promoter with an T->G mutation at the -17 position of the consensus sequence
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_R0181_sequence/1

BBa_R0183
type: ComponentDefinition
persistent identity: BBa R0183
display id: BBa_R0183
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: T7 RNAP promoter
creator: Barry Canton
created: 2005-09-25T11:00:00Z
modified: 2015-05-08T01:14:15Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_6_
m user id: 0
owner id: 135
owning group id: 6
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: T7 promoter with an A->C mutation at the -16 position of the consensus sequence
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_R0183_sequence/1

BBa_R0184
type: ComponentDefinition
persistent identity: BBa R0184
display id: BBa_R0184
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: T7 promoter (lacI repressible)
creator: Bartholomew Canton
created: 2007-04-25T11:00:00Z
modified: 2015-05-08T01:14:15Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_
m user id: 0
owner id: 135
owning group id: 84
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: A mutant version of the T7lac promoter designed by Dubendorff and Studier. The promoter is based on the strong ??10 T7 promoter but incorporates an A->C mutation at -16. The LacI operator site is a partially symmetric 25bp sequence.
mutable notes: The -16 mutation was included to weaken the consensus promoter as described by Imburgio and co-workers (see BBa_R0183 for more information)
mutable provenance: This sequence is based on the T7lac promoter described by Dubendorff and Studier (JMB 1991, 219, 45-59). The only modification is the mutation at -16.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: lacI binding site
sequence annotation: BBa_R0183 fragment
sequence annotation: Center of binding site symmetry
sequence: https://synbiohub.org/public/igem/BBa_R0184_sequence/1

lacI binding site
type: SequenceAnnotation
persistent identity: annotation1932679
display id: annotation1932679
version: 1
top level: BBa_R0184
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_R0184/annotation1932679/range1932679/1

https://synbiohub.org/public/igem/BBa_R0184/annotation1932679/range1932679/1
type: Range
persistent identity: range1932679
display id: range1932679
version: 1
direction: direction/forward
top level: BBa_R0184
start: 20
end: 44
orientation: inline

BBa_R0183 fragment
type: SequenceAnnotation
persistent identity: annotation1932678
display id: annotation1932678
version: 1
top level: BBa_R0184
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0184/annotation1932678/range1932678/1

https://synbiohub.org/public/igem/BBa_R0184/annotation1932678/range1932678/1
type: Range
persistent identity: range1932678
display id: range1932678
version: 1
direction: direction/forward
top level: BBa_R0184
start: 1
end: 20
orientation: inline

Center of binding site symmetry
type: SequenceAnnotation
persistent identity: annotation1932680
display id: annotation1932680
version: 1
top level: BBa_R0184
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_R0184/annotation1932680/range1932680/1

https://synbiohub.org/public/igem/BBa_R0184/annotation1932680/range1932680/1
type: Range
persistent identity: range1932680
display id: range1932680
version: 1
direction: direction/forward
top level: BBa_R0184
start: 31
end: 31
orientation: inline

BBa_K1614000
type: ComponentDefinition
persistent identity: BBa K1614000
display id: BBa_K1614000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: T7 promoter for expression of functional RNA
creator: Stefan Holderbach
created: 2015-09-13T11:00:00Z
modified: 2015-09-14T12:29:29Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2031_
m user id: 12380
owner id: 16422
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Test
mutable notes: None.
mutable provenance: T7 phage minimal promoter (consensus)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: T7 promoter
sequence: https://synbiohub.org/public/igem/BBa_K1614000_sequence/1

T7 promoter
type: SequenceAnnotation
persistent identity: annotation2454728
display id: annotation2454728
version: 1
top level: BBa_K1614000
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1614000/annotation2454728/range2454728/1

https://synbiohub.org/public/igem/BBa_K1614000/annotation2454728/range2454728/1
type: Range
persistent identity: range2454728
display id: range2454728
version: 1
direction: direction/forward
top level: BBa_K1614000
start: 1
end: 17
orientation: inline

BBa_K1833009
type: ComponentDefinition
persistent identity: BBa K1833009
display id: BBa_K1833009
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: T7 promoter -> 434 cI N terminus with P22 repressor specificity
creator: Konstantin Borisov
created: 2015-09-17T11:00:00Z
modified: 2015-09-17T11:13:57Z
discontinued: false
dominant: false
group u list: _2259_
m user id: 27388
owner id: 27388
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This part produces the DNA binding domain of the phage 434 cI repressor, with mutations that change the specificity of the DNA to which the protein binds. This protein domain was used as in "A two-hybrid system based on chimeric operator recognition for studying protein homo/heterodimerization in Escherichia coli" (G. Di Lallo, L. Castagnoli, P. Ghelardini and L. Paolozzi; Microbiology (2001), 147, 1651???1656). For more information on the uses of this part, visit 2015.igem.org/Team:Pitt/Protease/Project.
mutable notes: This part was designed with a T7 promoter so the protein could be overexpressed.
mutable provenance: "A two-hybrid system based on chimeric operator recognition for studying protein homo/heterodimerization in Escherichia coli" (G. Di Lallo, L. Castagnoli, P. Ghelardini and L. Paolozzi; Microbiology (2001), 147, 1651???1656) described the use of this protein domain.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: P22-like 434 cI N-terminus
sequence annotation: rbs
sequence annotation: pT7
sequence annotation: T7 terminator
sequence: https://synbiohub.org/public/igem/BBa_K1833009_sequence/1

P22-like 434 cI N-terminus
type: SequenceAnnotation
persistent identity: annotation2469007
display id: annotation2469007
version: 1
top level: BBa_K1833009
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1833009/annotation2469007/range2469007/1

https://synbiohub.org/public/igem/BBa_K1833009/annotation2469007/range2469007/1
type: Range
persistent identity: range2469007
display id: range2469007
version: 1
direction: direction/forward
top level: BBa_K1833009
start: 33
end: 317
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation2469005
display id: annotation2469005
version: 1
top level: BBa_K1833009
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1833009/annotation2469005/range2469005/1

https://synbiohub.org/public/igem/BBa_K1833009/annotation2469005/range2469005/1
type: Range
persistent identity: range2469005
display id: range2469005
version: 1
direction: direction/forward
top level: BBa_K1833009
start: 24
end: 29
orientation: inline

pT7
type: SequenceAnnotation
persistent identity: annotation2469004
display id: annotation2469004
version: 1
top level: BBa_K1833009
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1833009/annotation2469004/range2469004/1

https://synbiohub.org/public/igem/BBa_K1833009/annotation2469004/range2469004/1
type: Range
persistent identity: range2469004
display id: range2469004
version: 1
direction: direction/forward
top level: BBa_K1833009
start: 1
end: 20
orientation: inline

T7 terminator
type: SequenceAnnotation
persistent identity: annotation2469011
display id: annotation2469011
version: 1
top level: BBa_K1833009
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1833009/annotation2469011/range2469011/1

https://synbiohub.org/public/igem/BBa_K1833009/annotation2469011/range2469011/1
type: Range
persistent identity: range2469011
display id: range2469011
version: 1
direction: direction/forward
top level: BBa_K1833009
start: 318
end: 358
orientation: inline

BBa_Z0251
type: ComponentDefinition
persistent identity: BBa Z0251
display id: BBa_Z0251
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: T7 strong promoter
creator: T7.2
created: 2005-04-04T11:00:00Z
modified: 2015-05-08T01:14:55Z
discontinued: false
dominant: false
experience: experience/None
group u list: _10_
m user id: 0
owner id: 250
owning group id: 10
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Promoter for T7 RNA polymerase
mutable provenance: bacteriophage T7
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: consensus sequence
sequence: https://synbiohub.org/public/igem/BBa_Z0251_sequence/1

consensus sequence
type: SequenceAnnotation
persistent identity: annotation1476333
display id: annotation1476333
version: 1
top level: BBa_Z0251
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_Z0251/annotation1476333/range1476333/1

https://synbiohub.org/public/igem/BBa_Z0251/annotation1476333/range1476333/1
type: Range
persistent identity: range1476333
display id: range1476333
version: 1
direction: direction/forward
top level: BBa_Z0251
start: 6
end: 28
orientation: inline

BBa_R0182
type: ComponentDefinition
persistent identity: BBa R0182
display id: BBa_R0182
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: T7 RNAP promoter
creator: Barry Canton
created: 2005-09-25T11:00:00Z
modified: 2015-05-08T01:14:15Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_6_
m user id: 0
owner id: 135
owning group id: 6
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: T7 promoter with an A->T mutation at the -10 position of the consensus sequence
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_R0182_sequence/1

BBa_S03653
type: ComponentDefinition
persistent identity: BBa S03653
display id: BBa_S03653
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: E0042:S03650
creator: Heather Keller
created: 2007-03-05T12:00:00Z
modified: 2015-05-08T01:14:26Z
discontinued: false
dominant: false
experience: experience/None
group u list: _10_
m user id: 0
owner id: 571
owning group id: 10
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_B0012
component: BBa_B0010
component: BBa_B0101
component: BBa_E0042
sequence annotation: BBa_B0010
sequence annotation: BBa_B0101
sequence annotation: BBa_E0042
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_S03653_sequence/1

BBa_B0012
type: Component
persistent identity: component1920505
display id: component1920505
version: 1
top level: BBa_S03653
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0010
type: Component
persistent identity: component1920503
display id: component1920503
version: 1
top level: BBa_S03653
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0101
type: Component
persistent identity: component1920502
display id: component1920502
version: 1
top level: BBa_S03653
access: public
definition: https://synbiohub.org/public/igem/BBa_B0101/1

BBa_E0042
type: Component
persistent identity: component1920500
display id: component1920500
version: 1
top level: BBa_S03653
access: public
definition: https://synbiohub.org/public/igem/BBa_E0042/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1920503
display id: annotation1920503
version: 1
top level: BBa_S03653
location: https://synbiohub.org/public/igem/BBa_S03653/annotation1920503/range1920503/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_S03653/annotation1920503/range1920503/1
type: Range
persistent identity: range1920503
display id: range1920503
version: 1
direction: direction/forward
top level: BBa_S03653
start: 797
end: 876
orientation: inline

BBa_B0101
type: SequenceAnnotation
persistent identity: annotation1920502
display id: annotation1920502
version: 1
top level: BBa_S03653
location: https://synbiohub.org/public/igem/BBa_S03653/annotation1920502/range1920502/1
component: BBa_B0101

https://synbiohub.org/public/igem/BBa_S03653/annotation1920502/range1920502/1
type: Range
persistent identity: range1920502
display id: range1920502
version: 1
direction: direction/reverse
top level: BBa_S03653
start: 731
end: 788
orientation: inline

BBa_E0042
type: SequenceAnnotation
persistent identity: annotation1920500
display id: annotation1920500
version: 1
top level: BBa_S03653
location: https://synbiohub.org/public/igem/BBa_S03653/annotation1920500/range1920500/1
component: BBa_E0042

https://synbiohub.org/public/igem/BBa_S03653/annotation1920500/range1920500/1
type: Range
persistent identity: range1920500
display id: range1920500
version: 1
direction: direction/reverse
top level: BBa_S03653
start: 1
end: 722
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1920505
display id: annotation1920505
version: 1
top level: BBa_S03653
location: https://synbiohub.org/public/igem/BBa_S03653/annotation1920505/range1920505/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_S03653/annotation1920505/range1920505/1
type: Range
persistent identity: range1920505
display id: range1920505
version: 1
direction: direction/forward
top level: BBa_S03653
start: 885
end: 925
orientation: inline

BBa_K1833999
type: ComponentDefinition
persistent identity: BBa K1833999
display id: BBa_K1833999
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: T7 promoter as annealed oligos
creator: Konstantin Borisov
created: 2015-09-14T11:00:00Z
modified: 2015-09-16T07:58:33Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2259_
m user id: 27388
owner id: 27388
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This is a part used as a building block for T7 driven expression. However, this part uses a special method with the following synthesized nucleotides: pT7sense: aattcgcggccgcttctagataatacgactcactatagggagaa pT7antisense: ctagttctccctatagtgagtcgtattatctagaagcggccgcg The following method can be used to insert a T7 promoter before a desired Biobrick part: 1. Cut the desired plasmid (which should contain an RBS and cds at minimum) with EcoRI and XbaI. Purify the plasmid with a method of your choice (both gel purification and PCR purification kits from Qiagen serve to remove the short undesired oligonucleotide from the digestion). 2. Be sure that your oligos have 5' phosphorylation (treat unphosphorylated oligos with T4 Polynucleotide Kinase). Then, anneal the two oligos by slowly cooling from 98C to room temperature. 3. Finally, ligate the cut plasmid with the annealed oligos by using a 1:10 molar ratio of plasmid to insert. 4. Transform the ligation reaction into E. coli, and plate. 5. Pick colonies, and verify the success of the cloning with sequencing or PCR. (Doing a diagnostic digest is not recommended as the size of the original plasmid and the desired plasmid are very similar.) 6. Add a composite part to the iGEM registry by using this part with the original protein coding part.
mutable notes: When ligating with a cut plasmid, this leaves a Biobrick scar between the T7 promoter and original Biobrick part.
mutable provenance: Synthesized oligonucleotides as described on main page.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: pT7
sequence annotation: Oligo scar
sequence: https://synbiohub.org/public/igem/BBa_K1833999_sequence/1

pT7
type: SequenceAnnotation
persistent identity: annotation2460074
display id: annotation2460074
version: 1
top level: BBa_K1833999
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1833999/annotation2460074/range2460074/1

https://synbiohub.org/public/igem/BBa_K1833999/annotation2460074/range2460074/1
type: Range
persistent identity: range2460074
display id: range2460074
version: 1
direction: direction/forward
top level: BBa_K1833999
start: 1
end: 23
orientation: inline

Oligo scar
type: SequenceAnnotation
persistent identity: annotation2460116
display id: annotation2460116
version: 1
top level: BBa_K1833999
role: feature/scar
location: https://synbiohub.org/public/igem/BBa_K1833999/annotation2460116/range2460116/1

https://synbiohub.org/public/igem/BBa_K1833999/annotation2460116/range2460116/1
type: Range
persistent identity: range2460116
display id: range2460116
version: 1
direction: direction/forward
top level: BBa_K1833999
start: 24
end: 29
orientation: inline

pT7
type: ComponentDefinition
persistent identity: BBa I719005
display id: BBa_I719005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: T7 Promoter
creator: Imperial 2007
created: 2007-10-23T11:00:00Z
modified: 2015-08-31T04:07:53Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _128_
m user id: 0
owner id: 2097
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: Just a T7 Promoter
mutable notes: None
mutable provenance: ---
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I719005_sequence/1

BBa_I712074
type: ComponentDefinition
persistent identity: BBa I712074
display id: BBa_I712074
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: T7 promoter (strong promoter from T7 bacteriophage)
creator: Rok Gaber
created: 2007-10-21T11:00:00Z
modified: 2015-08-31T04:07:46Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _130_
m user id: 0
owner id: 1856
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: T7 promoter is very specific promoter which is transcribed only by specific T7 RNA polymerase. Usually this promoter is used in expression systems where T7 promoter is cotransfected with T7 RNA polymerase. That ensures strong transcription of desired genes.
mutable provenance: T7 bacteriophage
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I712074_sequence/1

BBa_R0186
type: ComponentDefinition
persistent identity: BBa R0186
display id: BBa_R0186
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: T7 promoter (lacI repressible)
creator: Bartholomew Canton
created: 2008-04-07T11:00:00Z
modified: 2015-05-08T01:14:15Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_
m user id: 0
owner id: 135
owning group id: 84
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: A lac repressible T7 promoter. This promoter is similar to those found in the pET vectors with T7lac promoters. I mutated the -9 base to an A, which should make it less than 3% of the strength of consensus. The base numbering is as used by Studier and coworkers (JMB 1991, 219, 45-59). See BBa_R0183 for more information.
mutable notes: The -9 mutation from consensus was included to weaken the consensus promoter as described by Imburgio and co-workers (see BBa_R0183 for more information)
mutable provenance: This sequence is based on the T7lac promoter described by Dubendorff and Studier (JMB 1991, 219, 45-59). The only modification is the mutation at -9.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: LacI binding site
sequence annotation: T7 promoter
sequence: https://synbiohub.org/public/igem/BBa_R0186_sequence/1

LacI binding site
type: SequenceAnnotation
persistent identity: annotation1962094
display id: annotation1962094
version: 1
top level: BBa_R0186
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0186/annotation1962094/range1962094/1

https://synbiohub.org/public/igem/BBa_R0186/annotation1962094/range1962094/1
type: Range
persistent identity: range1962094
display id: range1962094
version: 1
direction: direction/forward
top level: BBa_R0186
start: 20
end: 44
orientation: inline

T7 promoter
type: SequenceAnnotation
persistent identity: annotation1962095
display id: annotation1962095
version: 1
top level: BBa_R0186
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0186/annotation1962095/range1962095/1

https://synbiohub.org/public/igem/BBa_R0186/annotation1962095/range1962095/1
type: Range
persistent identity: range1962095
display id: range1962095
version: 1
direction: direction/forward
top level: BBa_R0186
start: 1
end: 20
orientation: inline

BBa_S03650
type: ComponentDefinition
persistent identity: BBa S03650
display id: BBa_S03650
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: B0100:B0015
creator: Heather Keller
created: 2007-03-05T12:00:00Z
modified: 2015-05-08T01:14:26Z
discontinued: false
dominant: false
experience: experience/None
group u list: _10_
m user id: 0
owner id: 571
owning group id: 10
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_B0012
component: BBa_B0101
component: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_B0101
sequence annotation: BBa_B0010
sequence: https://synbiohub.org/public/igem/BBa_S03650_sequence/1

BBa_B0012
type: Component
persistent identity: component1920491
display id: component1920491
version: 1
top level: BBa_S03650
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0101
type: Component
persistent identity: component1920488
display id: component1920488
version: 1
top level: BBa_S03650
access: public
definition: https://synbiohub.org/public/igem/BBa_B0101/1

BBa_B0010
type: Component
persistent identity: component1920489
display id: component1920489
version: 1
top level: BBa_S03650
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1920491
display id: annotation1920491
version: 1
top level: BBa_S03650
location: https://synbiohub.org/public/igem/BBa_S03650/annotation1920491/range1920491/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_S03650/annotation1920491/range1920491/1
type: Range
persistent identity: range1920491
display id: range1920491
version: 1
direction: direction/forward
top level: BBa_S03650
start: 155
end: 195
orientation: inline

BBa_B0101
type: SequenceAnnotation
persistent identity: annotation1920488
display id: annotation1920488
version: 1
top level: BBa_S03650
location: https://synbiohub.org/public/igem/BBa_S03650/annotation1920488/range1920488/1
component: BBa_B0101

https://synbiohub.org/public/igem/BBa_S03650/annotation1920488/range1920488/1
type: Range
persistent identity: range1920488
display id: range1920488
version: 1
direction: direction/reverse
top level: BBa_S03650
start: 1
end: 58
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1920489
display id: annotation1920489
version: 1
top level: BBa_S03650
location: https://synbiohub.org/public/igem/BBa_S03650/annotation1920489/range1920489/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_S03650/annotation1920489/range1920489/1
type: Range
persistent identity: range1920489
display id: range1920489
version: 1
direction: direction/forward
top level: BBa_S03650
start: 67
end: 146
orientation: inline

T7Gene6
type: ComponentDefinition
persistent identity: BBa K093001
display id: BBa_K093001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: T7 Gene 6 exonuclease
creator: John Heil, Shira Davis, Danielle Nash
created: 2008-10-25T11:00:00Z
modified: 2015-05-08T01:08:40Z
discontinued: false
dominant: false
experience: experience/None
group u list: _247_
m user id: 0
owner id: 3630
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This is the T7 gene 6 ORF, that codes for the T7 gene 6 5'-3' exonuclease. For properties of T7 gene 6 exonuclease see: Kerr and Sadowski "Gene 6 Exonuclease of Bacteriophage T7" The Journal of Biological Chemistry Vol. 247 No. 1 January 10, pp 311-318, 1972.
mutable notes: We needed to change the stop codon to the standard stop codon TAA. We later realized that we should have added tandem TAA. The Tandem TAA stop codon is present in the composite parts containing this ORF. The ORF alone has the single TAA stop codon.
mutable provenance: T7 genomic DNA
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K093001_sequence/1

BBa_K113012
type: ComponentDefinition
persistent identity: BBa K113012
display id: BBa_K113012
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: weaken overlapping T7 promoter
creator: Wu Jingjing, Wang Jinyu
created: 2008-10-25T11:00:00Z
modified: 2015-05-08T01:09:20Z
discontinued: false
dominant: false
experience: experience/None
group u list: _254_
m user id: 0
owner id: 2711
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Two T7 promoters are reversely overlapping in two base pairs while a point mutation occurs at the 12th base pair, from A-T to T-A.
mutable notes: It is difficult to make this short DNA by PCR as both sides are T7 promoters, which are very similar. It is highly possible that they form loops before they can be synthesized by PCR. Thus we order two single stranded DNA and then anneal them together by touchdown PCR.
mutable provenance: The sequence of T7 promoter is TAATACGACTCACTATA (from 5' to 3').
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: T7 promoter
sequence: https://synbiohub.org/public/igem/BBa_K113012_sequence/1

T7 promoter
type: SequenceAnnotation
persistent identity: annotation1989400
display id: annotation1989400
version: 1
top level: BBa_K113012
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K113012/annotation1989400/range1989400/1

https://synbiohub.org/public/igem/BBa_K113012/annotation1989400/range1989400/1
type: Range
persistent identity: range1989400
display id: range1989400
version: 1
direction: direction/reverse
top level: BBa_K113012
start: 4
end: 21
orientation: inline

T7 promoter
type: SequenceAnnotation
persistent identity: annotation1989402
display id: annotation1989402
version: 1
top level: BBa_K113012
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K113012/annotation1989402/range1989402/1

https://synbiohub.org/public/igem/BBa_K113012/annotation1989402/range1989402/1
type: Range
persistent identity: range1989402
display id: range1989402
version: 1
direction: direction/forward
top level: BBa_K113012
start: 20
end: 37
orientation: inline

BBa_K921000
type: ComponentDefinition
persistent identity: BBa K921000
display id: BBa_K921000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: T7 RNAP + IPTG->PoPs (Mutant I)
creator: Eric Pederson
created: 2012-09-30T11:00:00Z
modified: 2015-05-08T01:13:46Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1186_
m user id: 0
owner id: 12713
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: This is a mutant T7 promoter that includes a lacO sequence. This is repressible by the LacI protein and is induced in the presence of IPTG. The promoter sits in the 5' region of the gene of interest and initiates transcription when the cell expresses T7 RNA polymerase. Be sure you are using a cell strain that includes this gene (DE3).
mutable notes: There are three portions to the T7 promoter: the recognition site, the melting box (or TATA box) and the initation site. Mutations in these sequences caused differential behavior from the wild type.
mutable provenance: The part consists of a sequences that resemble a T7 promoter and the lacO sequence found in E. coli.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/T7
role: SO:0001207
sequence annotation: Initiation
sequence annotation: T7 promoter
sequence annotation: LacO
sequence: https://synbiohub.org/public/igem/BBa_K921000_sequence/1

Initiation
type: SequenceAnnotation
persistent identity: annotation2205291
display id: annotation2205291
version: 1
top level: BBa_K921000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K921000/annotation2205291/range2205291/1

https://synbiohub.org/public/igem/BBa_K921000/annotation2205291/range2205291/1
type: Range
persistent identity: range2205291
display id: range2205291
version: 1
direction: direction/forward
top level: BBa_K921000
start: 19
end: 20
orientation: inline

T7 promoter
type: SequenceAnnotation
persistent identity: annotation2205289
display id: annotation2205289
version: 1
top level: BBa_K921000
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K921000/annotation2205289/range2205289/1

https://synbiohub.org/public/igem/BBa_K921000/annotation2205289/range2205289/1
type: Range
persistent identity: range2205289
display id: range2205289
version: 1
direction: direction/forward
top level: BBa_K921000
start: 1
end: 19
orientation: inline

LacO
type: SequenceAnnotation
persistent identity: annotation2205290
display id: annotation2205290
version: 1
top level: BBa_K921000
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K921000/annotation2205290/range2205290/1

https://synbiohub.org/public/igem/BBa_K921000/annotation2205290/range2205290/1
type: Range
persistent identity: range2205290
display id: range2205290
version: 1
direction: direction/forward
top level: BBa_K921000
start: 19
end: 46
orientation: inline

BBa_K113010
type: ComponentDefinition
persistent identity: BBa K113010
display id: BBa_K113010
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: overlapping T7 promoter
creator: Wu Jingjing, Wang Jinyu
created: 2008-10-25T11:00:00Z
modified: 2015-05-08T01:09:20Z
discontinued: false
dominant: false
experience: experience/None
group u list: _254_
m user id: 0
owner id: 2711
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Two T7 promoters are reversely overlapping.
mutable notes: It is difficult to make this short DNA by PCR as both sides are T7 promoters, which are very similar. It is highly possible that they form loops before they can be synthesized by PCR. Thus we order two single stranded DNA and then anneal them together by touchdown PCR.
mutable provenance: The sequence of T7 promoter is TAATACGACTCACTATA.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: T7 promoter
sequence: https://synbiohub.org/public/igem/BBa_K113010_sequence/1

T7 promoter
type: SequenceAnnotation
persistent identity: annotation1989277
display id: annotation1989277
version: 1
top level: BBa_K113010
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K113010/annotation1989277/range1989277/1

https://synbiohub.org/public/igem/BBa_K113010/annotation1989277/range1989277/1
type: Range
persistent identity: range1989277
display id: range1989277
version: 1
direction: direction/reverse
top level: BBa_K113010
start: 4
end: 21
orientation: inline

T7 promoter
type: SequenceAnnotation
persistent identity: annotation1989307
display id: annotation1989307
version: 1
top level: BBa_K113010
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K113010/annotation1989307/range1989307/1

https://synbiohub.org/public/igem/BBa_K113010/annotation1989307/range1989307/1
type: Range
persistent identity: range1989307
display id: range1989307
version: 1
direction: direction/forward
top level: BBa_K113010
start: 20
end: 37
orientation: inline

BBa_J34814
type: ComponentDefinition
persistent identity: BBa J34814
display id: BBa_J34814
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: T7 Promoter
creator: Alexandra Choutko
created: 2006-10-25T11:00:00Z
modified: 2015-08-31T04:08:47Z
discontinued: false
dominant: false
experience: experience/None
group u list: _62_
m user id: 0
owner id: 1085
owning group id: 62
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: to be used qith t7 polymerase BBa_J34811
mutable notes: a
mutable provenance: a
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J34814_sequence/1

BBa_K921002
type: ComponentDefinition
persistent identity: BBa K921002
display id: BBa_K921002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: T7 RNAP + IPTG->PoPs (Mutant III)
creator: Eric Pederson
created: 2012-09-30T11:00:00Z
modified: 2015-05-08T01:13:46Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1186_
m user id: 0
owner id: 12713
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This is a mutant T7 promoter that includes a lacO sequence. This is repressible by the LacI protein and is induced in the presence of IPTG. The promoter sits in the 5' region of the gene of interest and initiates transcription when the cell expresses T7 RNA polymerase. Be sure you are using a cell strain that includes this gene (DE3).
mutable notes: There are three portions to the T7 promoter: the recognition site, the melting box (or TATA box) and the initation site. Mutations in these sequences caused differential behavior from the wild type.
mutable provenance: The part consists of a sequences that resemble a T7 promoter and the lacO sequence found in E. coli.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/T7
role: SO:0001207
sequence annotation: Initiation
sequence annotation: LacO
sequence annotation: T7 Promoter
sequence: https://synbiohub.org/public/igem/BBa_K921002_sequence/1

Initiation
type: SequenceAnnotation
persistent identity: annotation2205318
display id: annotation2205318
version: 1
top level: BBa_K921002
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K921002/annotation2205318/range2205318/1

https://synbiohub.org/public/igem/BBa_K921002/annotation2205318/range2205318/1
type: Range
persistent identity: range2205318
display id: range2205318
version: 1
direction: direction/forward
top level: BBa_K921002
start: 18
end: 20
orientation: inline

LacO
type: SequenceAnnotation
persistent identity: annotation2205317
display id: annotation2205317
version: 1
top level: BBa_K921002
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K921002/annotation2205317/range2205317/1

https://synbiohub.org/public/igem/BBa_K921002/annotation2205317/range2205317/1
type: Range
persistent identity: range2205317
display id: range2205317
version: 1
direction: direction/forward
top level: BBa_K921002
start: 19
end: 47
orientation: inline

T7 Promoter
type: SequenceAnnotation
persistent identity: annotation2205316
display id: annotation2205316
version: 1
top level: BBa_K921002
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K921002/annotation2205316/range2205316/1

https://synbiohub.org/public/igem/BBa_K921002/annotation2205316/range2205316/1
type: Range
persistent identity: range2205316
display id: range2205316
version: 1
direction: direction/forward
top level: BBa_K921002
start: 1
end: 20
orientation: inline

BBa_K921001
type: ComponentDefinition
persistent identity: BBa K921001
display id: BBa_K921001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: T7 RNAP + IPTG->PoPs (Mutant II)
creator: Eric Pederson
created: 2012-09-30T11:00:00Z
modified: 2015-05-08T01:13:46Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1186_
m user id: 0
owner id: 12713
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This is a mutant T7 promoter that includes a lacO sequence. This is repressible by the LacI protein and is induced in the presence of IPTG. The promoter sits in the 5' region of the gene of interest and initiates transcription when the cell expresses T7 RNA polymerase. Be sure you are using a cell strain that includes this gene (DE3).
mutable notes: There are three portions to the T7 promoter: the recognition site, the melting box (or TATA box) and the initation site. Mutations in these sequences caused differential behavior from the wild type.
mutable provenance: The part consists of a sequences that resemble a T7 promoter and the lacO sequence found in E. coli.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/T7
role: SO:0001207
sequence annotation: T7 Promoter
sequence annotation: LacO
sequence annotation: Initiation
sequence: https://synbiohub.org/public/igem/BBa_K921001_sequence/1

T7 Promoter
type: SequenceAnnotation
persistent identity: annotation2205296
display id: annotation2205296
version: 1
top level: BBa_K921001
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K921001/annotation2205296/range2205296/1

https://synbiohub.org/public/igem/BBa_K921001/annotation2205296/range2205296/1
type: Range
persistent identity: range2205296
display id: range2205296
version: 1
direction: direction/forward
top level: BBa_K921001
start: 1
end: 20
orientation: inline

LacO
type: SequenceAnnotation
persistent identity: annotation2205297
display id: annotation2205297
version: 1
top level: BBa_K921001
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K921001/annotation2205297/range2205297/1

https://synbiohub.org/public/igem/BBa_K921001/annotation2205297/range2205297/1
type: Range
persistent identity: range2205297
display id: range2205297
version: 1
direction: direction/forward
top level: BBa_K921001
start: 22
end: 48
orientation: inline

Initiation
type: SequenceAnnotation
persistent identity: annotation2205298
display id: annotation2205298
version: 1
top level: BBa_K921001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K921001/annotation2205298/range2205298/1

https://synbiohub.org/public/igem/BBa_K921001/annotation2205298/range2205298/1
type: Range
persistent identity: range2205298
display id: range2205298
version: 1
direction: direction/forward
top level: BBa_K921001
start: 18
end: 20
orientation: inline

BBa_J64997
type: ComponentDefinition
persistent identity: BBa J64997
display id: BBa_J64997
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: T7 consensus -10 and rest
creator: Sai Duriseti
created: 2007-03-25T11:00:00Z
modified: 2015-05-08T01:08:18Z
discontinued: false
dominant: false
experience: experience/None
group u list: _98_
m user id: 0
owner id: 1428
owning group id: 98
sample status: Not in stock
status: status/Unavailable
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: T7-10
sequence: https://synbiohub.org/public/igem/BBa_J64997_sequence/1

T7-10
type: SequenceAnnotation
persistent identity: annotation1921746
display id: annotation1921746
version: 1
top level: BBa_J64997
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_J64997/annotation1921746/range1921746/1

https://synbiohub.org/public/igem/BBa_J64997/annotation1921746/range1921746/1
type: Range
persistent identity: range1921746
display id: range1921746
version: 1
direction: direction/forward
top level: BBa_J64997
start: 1
end: 19
orientation: inline

BBa_K731700
type: ComponentDefinition
persistent identity: BBa K731700
display id: BBa_K731700
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Platform for terminators analysis under the control of T7 promoter
creator: Giacomo Giacomelli, Anna Depetris
created: 2012-08-13T11:00:00Z
modified: 2015-05-08T01:13:06Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _977_
m user id: 0
owner id: 12063
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: We used this part to test terminators: the two subsequent proteins allow to quantify the termination efficiency as the ratio between Venus's and Cherry's fluorescence. The vector is pET21b, that contains lacI, t7 promoter and ampicillin resistance gene. The two fluorescent proteins have partially overlapping emission spectra, thus the fluorimetric measures should be taken with these parameters, to avoid interference between the two: Venus excitation: 485nm, Venus emission: 528nm Cherry excitation: 528nm, Cherry emission: 615nm We inserted the prefix-suffix linker between the two proteins, to allow terminator characterization.
mutable notes: Using and modifying this construct is important to remember that the two proteins have strong N-terminal homology, especially designing primers. For any future improvement, maybe it would be better two use two different fluorescence proteins, because these two has partially overlapping emission spectra and different folding kinetics. Using and modifying this construct is important to remember that the two proteins have strong N-terminal homology, especially designing primers. For any future improvement, maybe it would be better two use two different fluorescence proteins, because these two has partially overlapping emission spectra and different folding kinetics.
mutable provenance: It's made from a Roberta Lentini's construct (from Mansy lab) that consist of pET21b with the two proteins and a 20bp linker between them. That construct was mutated two times to eliminate illegal restriction sites; prefix-suffix linker was added by insertion PCR.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: lacI
sequence annotation: T7 TERMINATOR
sequence annotation: ampicillin resistance gene
sequence annotation: mCHERRY
sequence annotation: lacO
sequence annotation: T7 PROMOTER
sequence annotation: A206K VENUS
sequence: https://synbiohub.org/public/igem/BBa_K731700_sequence/1

lacI
type: SequenceAnnotation
persistent identity: annotation2179559
display id: annotation2179559
version: 1
top level: BBa_K731700
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K731700/annotation2179559/range2179559/1

https://synbiohub.org/public/igem/BBa_K731700/annotation2179559/range2179559/1
type: Range
persistent identity: range2179559
display id: range2179559
version: 1
direction: direction/reverse
top level: BBa_K731700
start: 4562
end: 5653
orientation: inline

T7 TERMINATOR
type: SequenceAnnotation
persistent identity: annotation2179557
display id: annotation2179557
version: 1
top level: BBa_K731700
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K731700/annotation2179557/range2179557/1

https://synbiohub.org/public/igem/BBa_K731700/annotation2179557/range2179557/1
type: Range
persistent identity: range2179557
display id: range2179557
version: 1
direction: direction/forward
top level: BBa_K731700
start: 843
end: 890
orientation: inline

ampicillin resistance gene
type: SequenceAnnotation
persistent identity: annotation2179558
display id: annotation2179558
version: 1
top level: BBa_K731700
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K731700/annotation2179558/range2179558/1

https://synbiohub.org/public/igem/BBa_K731700/annotation2179558/range2179558/1
type: Range
persistent identity: range2179558
display id: range2179558
version: 1
direction: direction/forward
top level: BBa_K731700
start: 1711
end: 2370
orientation: inline

mCHERRY
type: SequenceAnnotation
persistent identity: annotation2179562
display id: annotation2179562
version: 1
top level: BBa_K731700
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K731700/annotation2179562/range2179562/1

https://synbiohub.org/public/igem/BBa_K731700/annotation2179562/range2179562/1
type: Range
persistent identity: range2179562
display id: range2179562
version: 1
direction: direction/forward
top level: BBa_K731700
start: 6119
end: 6829
orientation: inline

lacO
type: SequenceAnnotation
persistent identity: annotation2179561
display id: annotation2179561
version: 1
top level: BBa_K731700
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K731700/annotation2179561/range2179561/1

https://synbiohub.org/public/igem/BBa_K731700/annotation2179561/range2179561/1
type: Range
persistent identity: range2179561
display id: range2179561
version: 1
direction: direction/forward
top level: BBa_K731700
start: 6050
end: 6072
orientation: inline

T7 PROMOTER
type: SequenceAnnotation
persistent identity: annotation2179560
display id: annotation2179560
version: 1
top level: BBa_K731700
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K731700/annotation2179560/range2179560/1

https://synbiohub.org/public/igem/BBa_K731700/annotation2179560/range2179560/1
type: Range
persistent identity: range2179560
display id: range2179560
version: 1
direction: direction/forward
top level: BBa_K731700
start: 6031
end: 6050
orientation: inline

A206K VENUS
type: SequenceAnnotation
persistent identity: annotation2179556
display id: annotation2179556
version: 1
top level: BBa_K731700
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K731700/annotation2179556/range2179556/1

https://synbiohub.org/public/igem/BBa_K731700/annotation2179556/range2179556/1
type: Range
persistent identity: range2179556
display id: range2179556
version: 1
direction: direction/forward
top level: BBa_K731700
start: 36
end: 752
orientation: inline

BBa_R0085
type: ComponentDefinition
persistent identity: BBa R0085
display id: BBa_R0085
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: T7 Consensus Promoter Sequence
creator: Barry Canton
created: 2005-02-21T12:00:00Z
modified: 2015-05-08T01:14:15Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_6_
m user id: 0
owner id: 135
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: The T7 promoter should only produce PoPS when the T7 polymerase is also being expressed.
mutable provenance: Sequence obtained from Sri Kosuri
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: Initiation Region
sequence annotation: Polymerase Binding Region
sequence annotation: Transcription Start Site
sequence: https://synbiohub.org/public/igem/BBa_R0085_sequence/1

Initiation Region
type: SequenceAnnotation
persistent identity: annotation1721522
display id: annotation1721522
version: 1
top level: BBa_R0085
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_R0085/annotation1721522/range1721522/1

https://synbiohub.org/public/igem/BBa_R0085/annotation1721522/range1721522/1
type: Range
persistent identity: range1721522
display id: range1721522
version: 1
direction: direction/forward
top level: BBa_R0085
start: 12
end: 23
orientation: inline

Polymerase Binding Region
type: SequenceAnnotation
persistent identity: annotation1721521
display id: annotation1721521
version: 1
top level: BBa_R0085
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0085/annotation1721521/range1721521/1

https://synbiohub.org/public/igem/BBa_R0085/annotation1721521/range1721521/1
type: Range
persistent identity: range1721521
display id: range1721521
version: 1
direction: direction/forward
top level: BBa_R0085
start: 1
end: 11
orientation: inline

Transcription Start Site
type: SequenceAnnotation
persistent identity: annotation1721520
display id: annotation1721520
version: 1
top level: BBa_R0085
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_R0085/annotation1721520/range1721520/1

https://synbiohub.org/public/igem/BBa_R0085/annotation1721520/range1721520/1
type: Range
persistent identity: range1721520
display id: range1721520
version: 1
direction: direction/forward
top level: BBa_R0085
start: 18
end: 18
orientation: inline

BBa_K1833000
type: ComponentDefinition
persistent identity: BBa K1833000
display id: BBa_K1833000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pT7-eGFP
creator: Konstantin Borisov
created: 2015-09-12T11:00:00Z
modified: 2015-09-18T11:04:36Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2259_
m user id: 27388
owner id: 27388
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This part produces GFP in the presence of T7 RNA polymerase. It contains a T7 promoter, a strong RBS, the coding region for GFP, and a double terminator. The GFP is a mutant known as GFPmut3b, and the original citation is as follows: Cormack, B.P., Valdivia, R.H., and S. Falkow. FACS-optimized mutants of green fluorescent protein (GFP). Gene 173: 33-38 (1996). (http://www.sciencedirect.com/science/article/pii/0378111995006850) It has a maximum excitation at 501 nm and maximum emission at 511 nm. For more information, see part BBa_E0040
mutable notes: The part containing GFP was cut with EcoRI and XbaI, and ligated with the annealed oligos to form the completed part. Note that due to the choice of technique, a Biobrick scar site remains between the promoter and RBS.
mutable provenance: The RBS-GFP-terminator was obtained from as part BBa_E0840 from the 2015 Distribution (Plate 2, Well 24D). The T7 promoter was obtained as two short oligonucleotides annealed to form sticky ends. The part containing GFP was then cut with EcoRI and XbaI, and ligated with the annealed oligos to form the completed part. Note that due to the choice of technique, a Biobrick scar site remains between the promoter and RBS.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_K1833999
component: BBa_E0840
sequence annotation: BBa_E0840
sequence annotation: BBa_K1833999
sequence: https://synbiohub.org/public/igem/BBa_K1833000_sequence/1

BBa_K1833999
type: Component
persistent identity: component2460119
display id: component2460119
version: 1
top level: BBa_K1833000
access: public
definition: BBa_K1833999

BBa_E0840
type: Component
persistent identity: component2460130
display id: component2460130
version: 1
top level: BBa_K1833000
access: public
definition: https://synbiohub.org/public/igem/BBa_E0840/1

BBa_E0840
type: SequenceAnnotation
persistent identity: annotation2460130
display id: annotation2460130
version: 1
top level: BBa_K1833000
location: https://synbiohub.org/public/igem/BBa_K1833000/annotation2460130/range2460130/1
component: BBa_E0840

https://synbiohub.org/public/igem/BBa_K1833000/annotation2460130/range2460130/1
type: Range
persistent identity: range2460130
display id: range2460130
version: 1
direction: direction/reverse
top level: BBa_K1833000
start: 30
end: 907
orientation: inline

BBa_K1833999
type: SequenceAnnotation
persistent identity: annotation2460119
display id: annotation2460119
version: 1
top level: BBa_K1833000
location: https://synbiohub.org/public/igem/BBa_K1833000/annotation2460119/range2460119/1
component: BBa_K1833999

https://synbiohub.org/public/igem/BBa_K1833000/annotation2460119/range2460119/1
type: Range
persistent identity: range2460119
display id: range2460119
version: 1
direction: direction/reverse
top level: BBa_K1833000
start: 1
end: 29
orientation: inline