//chassis/eukaryote/human
type: Collection
persistent identity: chassis eukaryote human
display id: chassis_eukaryote_human
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
owned by: james
owned by: myers
member: BBa_K404250
member: BBa_K404006
member: BBa_K404164
member: flyPCD
member: BBa_K258002
member: BBa_K404241
member: BBa_K404155
member: BBa_K678005
member: BBa_J52021
member: BBa_K404209
member: BBa_K1119006
member: BBa_K404125
member: BBa_K404248
member: BBa_K404211
member: BBa_K404232
member: BBa_K404202
member: BBa_K404253
member: BBa_I712020
member: IscA2
member: BBa_K404227
member: BBa_K404206
member: BBa_K404167
member: BBa_K404223
member: BBa_K1777019
member: BBa_J52019
member: BBa_K404158
member: BBa_K1926022
member: BBa_I712002
member: BBa_K404108
member: BBa_K404011
member: BBa_K404235
member: BBa_K678022
member: BBa_K404101
member: BBa_K404203
member: BBa_K1926021
member: BBa_K404160
member: BBa_K404161
member: BBa_K404126
member: BBa_K258010
member: BBa_K404151
member: BBa_K1720004
member: BBa_K404121
member: BBa_K404254
member: BBa_K404168
member: BBa_K404224
member: BBa_K404205
member: BBa_K404119
member: BBa_J52013
member: BBa_K2061000
member: eGFP
member: BBa_K404128
member: BBa_K404012
member: BBa_I712024
member: VP64
member: BBa_K404003
member: BBa_K404207
member: BBa_K404166
member: BBa_K404153
member: BBa_K404123
member: BBa_K404243
member: BBa_K404256
member: BBa_K404104
member: BBa_K404213
member: BBa_K404117
member: BBa_K1936000
member: BBa_K1119008
member: hPCD
member: BBa_K1926013
member: BBa_K1926011
member: BBa_K404118
member: BBa_K404255

BBa_K678022
type: ComponentDefinition
persistent identity: BBa K678022
display id: BBa_K678022
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Neomycin cassette
creator: DTU-Denmark-2
created: 2011-09-03T11:00:00Z
modified: 2015-05-08T01:13:04Z
discontinued: false
dominant: false
experience: experience/None
group u list: _882_
m user id: 0
owner id: 8341
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Resistance cassette for selection of mammalian cells. The cassette contains a SV40 promoter, the neomycin resistance gene, and the SV40 poly A as a terminator.
mutable notes: Compatible with the Plug'n'Play assembly standard
mutable provenance: From plasmid pcDNA3.1 from Invitrogen
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Other
role: SO:0000110
sequence annotation: Linker 5
sequence annotation: Neomycin cassette
sequence: https://synbiohub.org/public/igem/BBa_K678022_sequence/1

Linker 5
type: SequenceAnnotation
persistent identity: annotation2127358
display id: annotation2127358
version: 1
top level: BBa_K678022
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K678022/annotation2127358/range2127358/1

https://synbiohub.org/public/igem/BBa_K678022/annotation2127358/range2127358/1
type: Range
persistent identity: range2127358
display id: range2127358
version: 1
direction: direction/forward
top level: BBa_K678022
start: 1
end: 8
orientation: inline

Neomycin cassette
type: SequenceAnnotation
persistent identity: annotation2127359
display id: annotation2127359
version: 1
top level: BBa_K678022
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K678022/annotation2127359/range2127359/1

https://synbiohub.org/public/igem/BBa_K678022/annotation2127359/range2127359/1
type: Range
persistent identity: range2127359
display id: range2127359
version: 1
direction: direction/forward
top level: BBa_K678022
start: 9
end: 1512
orientation: inline

BBa_K404118
type: ComponentDefinition
persistent identity: BBa K404118
display id: BBa_K404118
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: [AAV2]-left-ITR_phTERT_betaglobin
creator: Freiburg Bioware 2010
created: 2010-10-10T11:00:00Z
modified: 2015-05-08T01:12:23Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 6733
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: Beta-Globin-intron
sequence annotation: left ITR
sequence annotation: phTERT
sequence: https://synbiohub.org/public/igem/BBa_K404118_sequence/1

Beta-Globin-intron
type: SequenceAnnotation
persistent identity: annotation2093236
display id: annotation2093236
version: 1
top level: BBa_K404118
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404118/annotation2093236/range2093236/1

https://synbiohub.org/public/igem/BBa_K404118/annotation2093236/range2093236/1
type: Range
persistent identity: range2093236
display id: range2093236
version: 1
direction: direction/forward
top level: BBa_K404118
start: 619
end: 1113
orientation: inline

left ITR
type: SequenceAnnotation
persistent identity: annotation2093233
display id: annotation2093233
version: 1
top level: BBa_K404118
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404118/annotation2093233/range2093233/1

https://synbiohub.org/public/igem/BBa_K404118/annotation2093233/range2093233/1
type: Range
persistent identity: range2093233
display id: range2093233
version: 1
direction: direction/forward
top level: BBa_K404118
start: 1
end: 147
orientation: inline

phTERT
type: SequenceAnnotation
persistent identity: annotation2093235
display id: annotation2093235
version: 1
top level: BBa_K404118
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K404118/annotation2093235/range2093235/1

https://synbiohub.org/public/igem/BBa_K404118/annotation2093235/range2093235/1
type: Range
persistent identity: range2093235
display id: range2093235
version: 1
direction: direction/forward
top level: BBa_K404118
start: 154
end: 612
orientation: inline

BBa_K404214
type: ComponentDefinition
persistent identity: BBa K404214
display id: BBa_K404214
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: ViralBrick-587KO-Z34C-Spacer
creator: Freiburg Bioware 2010
created: 2010-08-27T11:00:00Z
modified: 2015-05-08T01:12:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 7049
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: upstream 453
sequence annotation: R=>A
sequence annotation: Z34C-motif
sequence annotation: TG Spacer
sequence annotation: GLS Spacer
sequence annotation: upstream 587
sequence annotation: downstream 587
sequence annotation: downstream 453
sequence: https://synbiohub.org/public/igem/BBa_K404214_sequence/1

upstream 453
type: SequenceAnnotation
persistent identity: annotation2079164
display id: annotation2079164
version: 1
top level: BBa_K404214
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404214/annotation2079164/range2079164/1

https://synbiohub.org/public/igem/BBa_K404214/annotation2079164/range2079164/1
type: Range
persistent identity: range2079164
display id: range2079164
version: 1
direction: direction/forward
top level: BBa_K404214
start: 1
end: 9
orientation: inline

R=>A
type: SequenceAnnotation
persistent identity: annotation2079172
display id: annotation2079172
version: 1
top level: BBa_K404214
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K404214/annotation2079172/range2079172/1

https://synbiohub.org/public/igem/BBa_K404214/annotation2079172/range2079172/1
type: Range
persistent identity: range2079172
display id: range2079172
version: 1
direction: direction/forward
top level: BBa_K404214
start: 160
end: 162
orientation: inline

Z34C-motif
type: SequenceAnnotation
persistent identity: annotation2079168
display id: annotation2079168
version: 1
top level: BBa_K404214
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404214/annotation2079168/range2079168/1

https://synbiohub.org/public/igem/BBa_K404214/annotation2079168/range2079168/1
type: Range
persistent identity: range2079168
display id: range2079168
version: 1
direction: direction/forward
top level: BBa_K404214
start: 49
end: 150
orientation: inline

TG Spacer
type: SequenceAnnotation
persistent identity: annotation2079167
display id: annotation2079167
version: 1
top level: BBa_K404214
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404214/annotation2079167/range2079167/1

https://synbiohub.org/public/igem/BBa_K404214/annotation2079167/range2079167/1
type: Range
persistent identity: range2079167
display id: range2079167
version: 1
direction: direction/forward
top level: BBa_K404214
start: 43
end: 48
orientation: inline

GLS Spacer
type: SequenceAnnotation
persistent identity: annotation2079169
display id: annotation2079169
version: 1
top level: BBa_K404214
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404214/annotation2079169/range2079169/1

https://synbiohub.org/public/igem/BBa_K404214/annotation2079169/range2079169/1
type: Range
persistent identity: range2079169
display id: range2079169
version: 1
direction: direction/forward
top level: BBa_K404214
start: 151
end: 159
orientation: inline

upstream 587
type: SequenceAnnotation
persistent identity: annotation2079165
display id: annotation2079165
version: 1
top level: BBa_K404214
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404214/annotation2079165/range2079165/1

https://synbiohub.org/public/igem/BBa_K404214/annotation2079165/range2079165/1
type: Range
persistent identity: range2079165
display id: range2079165
version: 1
direction: direction/forward
top level: BBa_K404214
start: 10
end: 42
orientation: inline

R=>A
type: SequenceAnnotation
persistent identity: annotation2079166
display id: annotation2079166
version: 1
top level: BBa_K404214
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K404214/annotation2079166/range2079166/1

https://synbiohub.org/public/igem/BBa_K404214/annotation2079166/range2079166/1
type: Range
persistent identity: range2079166
display id: range2079166
version: 1
direction: direction/forward
top level: BBa_K404214
start: 34
end: 36
orientation: inline

downstream 587
type: SequenceAnnotation
persistent identity: annotation2079170
display id: annotation2079170
version: 1
top level: BBa_K404214
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404214/annotation2079170/range2079170/1

https://synbiohub.org/public/igem/BBa_K404214/annotation2079170/range2079170/1
type: Range
persistent identity: range2079170
display id: range2079170
version: 1
direction: direction/forward
top level: BBa_K404214
start: 160
end: 180
orientation: inline

downstream 453
type: SequenceAnnotation
persistent identity: annotation2079171
display id: annotation2079171
version: 1
top level: BBa_K404214
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404214/annotation2079171/range2079171/1

https://synbiohub.org/public/igem/BBa_K404214/annotation2079171/range2079171/1
type: Range
persistent identity: range2079171
display id: range2079171
version: 1
direction: direction/forward
top level: BBa_K404214
start: 181
end: 189
orientation: inline

BBa_K404213
type: ComponentDefinition
persistent identity: BBa K404213
display id: BBa_K404213
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: ViralBrick-587KO-Z34C
creator: Freiburg Bioware 2010
created: 2010-08-27T11:00:00Z
modified: 2015-05-08T01:12:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 7049
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: downstream 453
sequence annotation: upstream 587
sequence annotation: R=>A
sequence annotation: upstream 453
sequence annotation: downstream 587
sequence annotation: Z34C-motif
sequence: https://synbiohub.org/public/igem/BBa_K404213_sequence/1

downstream 453
type: SequenceAnnotation
persistent identity: annotation2079163
display id: annotation2079163
version: 1
top level: BBa_K404213
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404213/annotation2079163/range2079163/1

https://synbiohub.org/public/igem/BBa_K404213/annotation2079163/range2079163/1
type: Range
persistent identity: range2079163
display id: range2079163
version: 1
direction: direction/forward
top level: BBa_K404213
start: 166
end: 174
orientation: inline

upstream 587
type: SequenceAnnotation
persistent identity: annotation2079158
display id: annotation2079158
version: 1
top level: BBa_K404213
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404213/annotation2079158/range2079158/1

https://synbiohub.org/public/igem/BBa_K404213/annotation2079158/range2079158/1
type: Range
persistent identity: range2079158
display id: range2079158
version: 1
direction: direction/forward
top level: BBa_K404213
start: 10
end: 42
orientation: inline

R=>A
type: SequenceAnnotation
persistent identity: annotation2079161
display id: annotation2079161
version: 1
top level: BBa_K404213
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K404213/annotation2079161/range2079161/1

https://synbiohub.org/public/igem/BBa_K404213/annotation2079161/range2079161/1
type: Range
persistent identity: range2079161
display id: range2079161
version: 1
direction: direction/forward
top level: BBa_K404213
start: 145
end: 147
orientation: inline

upstream 453
type: SequenceAnnotation
persistent identity: annotation2079157
display id: annotation2079157
version: 1
top level: BBa_K404213
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404213/annotation2079157/range2079157/1

https://synbiohub.org/public/igem/BBa_K404213/annotation2079157/range2079157/1
type: Range
persistent identity: range2079157
display id: range2079157
version: 1
direction: direction/forward
top level: BBa_K404213
start: 1
end: 9
orientation: inline

R=>A
type: SequenceAnnotation
persistent identity: annotation2079159
display id: annotation2079159
version: 1
top level: BBa_K404213
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K404213/annotation2079159/range2079159/1

https://synbiohub.org/public/igem/BBa_K404213/annotation2079159/range2079159/1
type: Range
persistent identity: range2079159
display id: range2079159
version: 1
direction: direction/forward
top level: BBa_K404213
start: 34
end: 36
orientation: inline

downstream 587
type: SequenceAnnotation
persistent identity: annotation2079162
display id: annotation2079162
version: 1
top level: BBa_K404213
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404213/annotation2079162/range2079162/1

https://synbiohub.org/public/igem/BBa_K404213/annotation2079162/range2079162/1
type: Range
persistent identity: range2079162
display id: range2079162
version: 1
direction: direction/forward
top level: BBa_K404213
start: 145
end: 165
orientation: inline

Z34C-motif
type: SequenceAnnotation
persistent identity: annotation2079160
display id: annotation2079160
version: 1
top level: BBa_K404213
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404213/annotation2079160/range2079160/1

https://synbiohub.org/public/igem/BBa_K404213/annotation2079160/range2079160/1
type: Range
persistent identity: range2079160
display id: range2079160
version: 1
direction: direction/forward
top level: BBa_K404213
start: 43
end: 144
orientation: inline

BBa_K404254
type: ComponentDefinition
persistent identity: BBa K404254
display id: BBa_K404254
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pCMV_[AAV2]-VP123ex (ViralBrick-587KO-RGD)
creator: Freiburg Bioware 2010
created: 2010-10-13T11:00:00Z
modified: 2015-05-08T01:12:25Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 7049
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: CMV
sequence annotation: GLS-spacer
sequence annotation: VP1
sequence annotation: VP3
sequence annotation: TG-spacer
sequence annotation: downstream 587
sequence annotation: VP2
sequence annotation: HSPG-KO
sequence annotation: AAV-2-cap (Stratagene)
sequence annotation: upstream 587
sequence annotation: RGD
sequence: https://synbiohub.org/public/igem/BBa_K404254_sequence/1

CMV
type: SequenceAnnotation
persistent identity: annotation2094679
display id: annotation2094679
version: 1
top level: BBa_K404254
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K404254/annotation2094679/range2094679/1

https://synbiohub.org/public/igem/BBa_K404254/annotation2094679/range2094679/1
type: Range
persistent identity: range2094679
display id: range2094679
version: 1
direction: direction/forward
top level: BBa_K404254
start: 2
end: 655
orientation: inline

GLS-spacer
type: SequenceAnnotation
persistent identity: annotation2094686
display id: annotation2094686
version: 1
top level: BBa_K404254
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404254/annotation2094686/range2094686/1

https://synbiohub.org/public/igem/BBa_K404254/annotation2094686/range2094686/1
type: Range
persistent identity: range2094686
display id: range2094686
version: 1
direction: direction/forward
top level: BBa_K404254
start: 2462
end: 2470
orientation: inline

VP1
type: SequenceAnnotation
persistent identity: annotation2094680
display id: annotation2094680
version: 1
top level: BBa_K404254
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404254/annotation2094680/range2094680/1

https://synbiohub.org/public/igem/BBa_K404254/annotation2094680/range2094680/1
type: Range
persistent identity: range2094680
display id: range2094680
version: 1
direction: direction/forward
top level: BBa_K404254
start: 671
end: 2917
orientation: inline

VP3
type: SequenceAnnotation
persistent identity: annotation2094682
display id: annotation2094682
version: 1
top level: BBa_K404254
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404254/annotation2094682/range2094682/1

https://synbiohub.org/public/igem/BBa_K404254/annotation2094682/range2094682/1
type: Range
persistent identity: range2094682
display id: range2094682
version: 1
direction: direction/forward
top level: BBa_K404254
start: 1274
end: 2917
orientation: inline

TG-spacer
type: SequenceAnnotation
persistent identity: annotation2094685
display id: annotation2094685
version: 1
top level: BBa_K404254
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404254/annotation2094685/range2094685/1

https://synbiohub.org/public/igem/BBa_K404254/annotation2094685/range2094685/1
type: Range
persistent identity: range2094685
display id: range2094685
version: 1
direction: direction/forward
top level: BBa_K404254
start: 2429
end: 2434
orientation: inline

downstream 587
type: SequenceAnnotation
persistent identity: annotation2094687
display id: annotation2094687
version: 1
top level: BBa_K404254
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404254/annotation2094687/range2094687/1

https://synbiohub.org/public/igem/BBa_K404254/annotation2094687/range2094687/1
type: Range
persistent identity: range2094687
display id: range2094687
version: 1
direction: direction/forward
top level: BBa_K404254
start: 2471
end: 2486
orientation: inline

VP2
type: SequenceAnnotation
persistent identity: annotation2094681
display id: annotation2094681
version: 1
top level: BBa_K404254
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404254/annotation2094681/range2094681/1

https://synbiohub.org/public/igem/BBa_K404254/annotation2094681/range2094681/1
type: Range
persistent identity: range2094681
display id: range2094681
version: 1
direction: direction/forward
top level: BBa_K404254
start: 1079
end: 2917
orientation: inline

HSPG-KO
type: SequenceAnnotation
persistent identity: annotation2101886
display id: annotation2101886
version: 1
top level: BBa_K404254
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K404254/annotation2101886/range2101886/1

https://synbiohub.org/public/igem/BBa_K404254/annotation2101886/range2101886/1
type: Range
persistent identity: range2101886
display id: range2101886
version: 1
direction: direction/forward
top level: BBa_K404254
start: 2471
end: 2473
orientation: inline

AAV-2-cap (Stratagene)
type: SequenceAnnotation
persistent identity: annotation2094683
display id: annotation2094683
version: 1
top level: BBa_K404254
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404254/annotation2094683/range2094683/1

https://synbiohub.org/public/igem/BBa_K404254/annotation2094683/range2094683/1
type: Range
persistent identity: range2094683
display id: range2094683
version: 1
direction: direction/forward
top level: BBa_K404254
start: 671
end: 3040
orientation: inline

HSPG-KO
type: SequenceAnnotation
persistent identity: annotation2101887
display id: annotation2101887
version: 1
top level: BBa_K404254
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K404254/annotation2101887/range2101887/1

https://synbiohub.org/public/igem/BBa_K404254/annotation2101887/range2101887/1
type: Range
persistent identity: range2101887
display id: range2101887
version: 1
direction: direction/forward
top level: BBa_K404254
start: 2433
end: 2435
orientation: inline

upstream 587
type: SequenceAnnotation
persistent identity: annotation2094688
display id: annotation2094688
version: 1
top level: BBa_K404254
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404254/annotation2094688/range2094688/1

https://synbiohub.org/public/igem/BBa_K404254/annotation2094688/range2094688/1
type: Range
persistent identity: range2094688
display id: range2094688
version: 1
direction: direction/forward
top level: BBa_K404254
start: 2397
end: 2428
orientation: inline

RGD
type: SequenceAnnotation
persistent identity: annotation2094684
display id: annotation2094684
version: 1
top level: BBa_K404254
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404254/annotation2094684/range2094684/1

https://synbiohub.org/public/igem/BBa_K404254/annotation2094684/range2094684/1
type: Range
persistent identity: range2094684
display id: range2094684
version: 1
direction: direction/forward
top level: BBa_K404254
start: 2435
end: 2461
orientation: inline

BBa_M1436
type: ComponentDefinition
persistent identity: BBa M1436
display id: BBa_M1436
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Pepsinogen from Homo sapiens optimized for E. coli
creator: Trevor Russell Ballard
created: 2013-04-24T11:00:00Z
modified: 2015-05-08T01:13:56Z
discontinued: false
dominant: false
group u list: _768_
m user id: 0
owner id: 17280
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Pepsinogen is the precursor protein for the enzyme pepsin, one of the primary digestive enzymes located in the human stomach. Pepsinogen activates into pepsin in the presence of HCl and other pepsinogen molecules. It is a protease, being used in the human digestive system to digest proteins. This sequence has been codon optimized for expression in E. coli.
mutable notes: Codon optimized for expression in E. coli using Gene Designer from DNA 2.0.
mutable provenance: http://www.uniprot.org/uniprot/P0DJD7
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_M1436_sequence/1

BBa_K404204
type: ComponentDefinition
persistent identity: BBa K404204
display id: BBa_K404204
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: ViralBrick-453-Z34C
creator: Freiburg Bioware 2010
created: 2010-08-27T11:00:00Z
modified: 2015-05-08T01:12:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 7049
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: Z34C
sequence annotation: upstream 453
sequence annotation: downstream 453
sequence: https://synbiohub.org/public/igem/BBa_K404204_sequence/1

Z34C
type: SequenceAnnotation
persistent identity: annotation2078945
display id: annotation2078945
version: 1
top level: BBa_K404204
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404204/annotation2078945/range2078945/1

https://synbiohub.org/public/igem/BBa_K404204/annotation2078945/range2078945/1
type: Range
persistent identity: range2078945
display id: range2078945
version: 1
direction: direction/forward
top level: BBa_K404204
start: 43
end: 144
orientation: inline

upstream 453
type: SequenceAnnotation
persistent identity: annotation2078944
display id: annotation2078944
version: 1
top level: BBa_K404204
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404204/annotation2078944/range2078944/1

https://synbiohub.org/public/igem/BBa_K404204/annotation2078944/range2078944/1
type: Range
persistent identity: range2078944
display id: range2078944
version: 1
direction: direction/forward
top level: BBa_K404204
start: 1
end: 42
orientation: inline

downstream 453
type: SequenceAnnotation
persistent identity: annotation2078946
display id: annotation2078946
version: 1
top level: BBa_K404204
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404204/annotation2078946/range2078946/1

https://synbiohub.org/public/igem/BBa_K404204/annotation2078946/range2078946/1
type: Range
persistent identity: range2078946
display id: range2078946
version: 1
direction: direction/forward
top level: BBa_K404204
start: 145
end: 165
orientation: inline

BBa_K404159
type: ComponentDefinition
persistent identity: BBa K404159
display id: BBa_K404159
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pCMV_CFP_Middle-Linker_[AAV2]-VP23 (ViralBrick-587KO-Empty)
creator: Freiburg Bioware 2010
created: 2010-10-11T11:00:00Z
modified: 2015-05-08T01:12:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 6733
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: CMV
sequence annotation: VP3
sequence annotation: HSPG-KO
sequence annotation: CFP
sequence annotation: VP2
sequence annotation: middle-linker
sequence annotation: AAV-2-cap (Stratagene)
sequence: https://synbiohub.org/public/igem/BBa_K404159_sequence/1

CMV
type: SequenceAnnotation
persistent identity: annotation2097914
display id: annotation2097914
version: 1
top level: BBa_K404159
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K404159/annotation2097914/range2097914/1

https://synbiohub.org/public/igem/BBa_K404159/annotation2097914/range2097914/1
type: Range
persistent identity: range2097914
display id: range2097914
version: 1
direction: direction/forward
top level: BBa_K404159
start: 2
end: 655
orientation: inline

VP3
type: SequenceAnnotation
persistent identity: annotation2097918
display id: annotation2097918
version: 1
top level: BBa_K404159
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404159/annotation2097918/range2097918/1

https://synbiohub.org/public/igem/BBa_K404159/annotation2097918/range2097918/1
type: Range
persistent identity: range2097918
display id: range2097918
version: 1
direction: direction/forward
top level: BBa_K404159
start: 1613
end: 3214
orientation: inline

HSPG-KO
type: SequenceAnnotation
persistent identity: annotation2101798
display id: annotation2101798
version: 1
top level: BBa_K404159
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K404159/annotation2101798/range2101798/1

https://synbiohub.org/public/igem/BBa_K404159/annotation2101798/range2101798/1
type: Range
persistent identity: range2101798
display id: range2101798
version: 1
direction: direction/forward
top level: BBa_K404159
start: 2759
end: 2761
orientation: inline

CFP
type: SequenceAnnotation
persistent identity: annotation2097915
display id: annotation2097915
version: 1
top level: BBa_K404159
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404159/annotation2097915/range2097915/1

https://synbiohub.org/public/igem/BBa_K404159/annotation2097915/range2097915/1
type: Range
persistent identity: range2097915
display id: range2097915
version: 1
direction: direction/forward
top level: BBa_K404159
start: 662
end: 1385
orientation: inline

VP2
type: SequenceAnnotation
persistent identity: annotation2097917
display id: annotation2097917
version: 1
top level: BBa_K404159
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404159/annotation2097917/range2097917/1

https://synbiohub.org/public/igem/BBa_K404159/annotation2097917/range2097917/1
type: Range
persistent identity: range2097917
display id: range2097917
version: 1
direction: direction/forward
top level: BBa_K404159
start: 1421
end: 3214
orientation: inline

HSPG-KO
type: SequenceAnnotation
persistent identity: annotation2101797
display id: annotation2101797
version: 1
top level: BBa_K404159
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K404159/annotation2101797/range2101797/1

https://synbiohub.org/public/igem/BBa_K404159/annotation2101797/range2101797/1
type: Range
persistent identity: range2101797
display id: range2101797
version: 1
direction: direction/forward
top level: BBa_K404159
start: 2768
end: 2770
orientation: inline

middle-linker
type: SequenceAnnotation
persistent identity: annotation2097916
display id: annotation2097916
version: 1
top level: BBa_K404159
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404159/annotation2097916/range2097916/1

https://synbiohub.org/public/igem/BBa_K404159/annotation2097916/range2097916/1
type: Range
persistent identity: range2097916
display id: range2097916
version: 1
direction: direction/forward
top level: BBa_K404159
start: 1391
end: 1414
orientation: inline

AAV-2-cap (Stratagene)
type: SequenceAnnotation
persistent identity: annotation2097919
display id: annotation2097919
version: 1
top level: BBa_K404159
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404159/annotation2097919/range2097919/1

https://synbiohub.org/public/igem/BBa_K404159/annotation2097919/range2097919/1
type: Range
persistent identity: range2097919
display id: range2097919
version: 1
direction: direction/forward
top level: BBa_K404159
start: 1421
end: 3337
orientation: inline

flyPCD
type: ComponentDefinition
persistent identity: BBa J176001
display id: BBa_J176001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: flyPCD
creator: Karmella Haynes
created: 2011-07-01T11:00:00Z
modified: 2015-08-31T04:08:33Z
discontinued: false
dominant: false
experience: experience/None
group u list: _863_
m user id: 0
owner id: 1144
owning group id: 61
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Amino acids 2-64 from the zebrafish CBX2 protein. This is the conserved Polycomb chromodomain predicted to bind trimethylated histone H3 lysine 27 in chromatin.
mutable notes: Codons were optimized for expression in cultured human cells.
mutable provenance: Danio rerio (zebrafish) CBX2 amino acids 2-64. The human codon-optimized coding sequence was synthesized by Genscript.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence: https://synbiohub.org/public/igem/BBa_J176001_sequence/1

BBa_K1119008
type: ComponentDefinition
persistent identity: BBa K1119008
display id: BBa_K1119008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CMV promoter - GFP - hGH polyA tail
creator: Hyun Jung Lee
created: 2013-09-15T11:00:00Z
modified: 2015-05-08T01:09:13Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1430_
m user id: 0
owner id: 17064
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: Later
mutable notes: Later
mutable provenance: Later
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: GFP
sequence annotation: hGH plolya
sequence annotation: BBa_K648013
sequence annotation: BBa_K1119006
sequence annotation: CMV promoter
sequence annotation: BBa_K404108
sequence: https://synbiohub.org/public/igem/BBa_K1119008_sequence/1

GFP
type: SequenceAnnotation
persistent identity: annotation2363281
display id: annotation2363281
version: 1
top level: BBa_K1119008
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1119008/annotation2363281/range2363281/1

https://synbiohub.org/public/igem/BBa_K1119008/annotation2363281/range2363281/1
type: Range
persistent identity: range2363281
display id: range2363281
version: 1
direction: direction/forward
top level: BBa_K1119008
start: 634
end: 1368
orientation: inline

hGH plolya
type: SequenceAnnotation
persistent identity: annotation2363282
display id: annotation2363282
version: 1
top level: BBa_K1119008
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_K1119008/annotation2363282/range2363282/1

https://synbiohub.org/public/igem/BBa_K1119008/annotation2363282/range2363282/1
type: Range
persistent identity: range2363282
display id: range2363282
version: 1
direction: direction/forward
top level: BBa_K1119008
start: 1386
end: 1866
orientation: inline

BBa_K648013
type: SequenceAnnotation
persistent identity: annotation2363277
display id: annotation2363277
version: 1
top level: BBa_K1119008
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1119008/annotation2363277/range2363277/1

https://synbiohub.org/public/igem/BBa_K1119008/annotation2363277/range2363277/1
type: Range
persistent identity: range2363277
display id: range2363277
version: 1
direction: direction/forward
top level: BBa_K1119008
start: 634
end: 1368
orientation: inline

BBa_K1119006
type: SequenceAnnotation
persistent identity: annotation2363276
display id: annotation2363276
version: 1
top level: BBa_K1119008
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1119008/annotation2363276/range2363276/1

https://synbiohub.org/public/igem/BBa_K1119008/annotation2363276/range2363276/1
type: Range
persistent identity: range2363276
display id: range2363276
version: 1
direction: direction/forward
top level: BBa_K1119008
start: 1
end: 618
orientation: inline

CMV promoter
type: SequenceAnnotation
persistent identity: annotation2363280
display id: annotation2363280
version: 1
top level: BBa_K1119008
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1119008/annotation2363280/range2363280/1

https://synbiohub.org/public/igem/BBa_K1119008/annotation2363280/range2363280/1
type: Range
persistent identity: range2363280
display id: range2363280
version: 1
direction: direction/forward
top level: BBa_K1119008
start: 1
end: 618
orientation: inline

BBa_K404108
type: SequenceAnnotation
persistent identity: annotation2363278
display id: annotation2363278
version: 1
top level: BBa_K1119008
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1119008/annotation2363278/range2363278/1

https://synbiohub.org/public/igem/BBa_K1119008/annotation2363278/range2363278/1
type: Range
persistent identity: range2363278
display id: range2363278
version: 1
direction: direction/forward
top level: BBa_K1119008
start: 1386
end: 1866
orientation: inline

BBa_K404012
type: ComponentDefinition
persistent identity: BBa K404012
display id: BBa_K404012
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: [AAV2]-VP3
creator: Freiburg Bioware 2010
created: 2010-10-20T11:00:00Z
modified: 2015-05-08T01:12:23Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 6674
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: VP3
sequence: https://synbiohub.org/public/igem/BBa_K404012_sequence/1

VP3
type: SequenceAnnotation
persistent identity: annotation2109186
display id: annotation2109186
version: 1
top level: BBa_K404012
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404012/annotation2109186/range2109186/1

https://synbiohub.org/public/igem/BBa_K404012/annotation2109186/range2109186/1
type: Range
persistent identity: range2109186
display id: range2109186
version: 1
direction: direction/forward
top level: BBa_K404012
start: 1
end: 1635
orientation: inline

BBa_K404107
type: ComponentDefinition
persistent identity: BBa K404107
display id: BBa_K404107
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Beta-Globin-Intron
creator: Freiburg Bioware 2010
created: 2010-08-27T11:00:00Z
modified: 2015-05-08T01:12:23Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 7049
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: Beta-Globin-intron
sequence: https://synbiohub.org/public/igem/BBa_K404107_sequence/1

Beta-Globin-intron
type: SequenceAnnotation
persistent identity: annotation2093172
display id: annotation2093172
version: 1
top level: BBa_K404107
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404107/annotation2093172/range2093172/1

https://synbiohub.org/public/igem/BBa_K404107/annotation2093172/range2093172/1
type: Range
persistent identity: range2093172
display id: range2093172
version: 1
direction: direction/forward
top level: BBa_K404107
start: 1
end: 495
orientation: inline

VP64
type: ComponentDefinition
persistent identity: BBa K1982012
display id: BBa_K1982012
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: VP64 transcription activitor
creator: Zexu Li
created: 2016-10-17T11:00:00Z
modified: 2016-10-18T09:58:31Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2449_
m user id: 30176
owner id: 30176
owning group id: 9
status: status/Planning
bookmark: false
mutable description: {| style="color:black" cellpadding="6" cellspacing="1" border="2" align="right" ! colspan="2" style="background:#FFBF00;"|VP64 |- |'''Function''' |transcription activator |- |'''RFC standard''' |[http://parts.igem.org/Help:Assembly_standard_10 RFC 10] |- |'''Backbone''' |pSB1C3 |- |'''Organism''' |''synthetic construct'' |- |'''Source''' |synthetic construct |- |'''Submitted by''' |[http://2016.igem.org/Team:NEU-China NEU-China 2016] |} Tetrameric VP16 transcription activator domain
mutable notes: synthetic construct
mutable provenance: synthetic construct
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: FLAG
sequence annotation: VP16
sequence annotation: SV40 NLS
sequence annotation: HA
sequence annotation: TAATAA
sequence annotation: Linker
sequence: https://synbiohub.org/public/igem/BBa_K1982012_sequence/1

FLAG
type: SequenceAnnotation
persistent identity: annotation2517156
display id: annotation2517156
version: 1
top level: VP64
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1982012/annotation2517156/range2517156/1

https://synbiohub.org/public/igem/BBa_K1982012/annotation2517156/range2517156/1
type: Range
persistent identity: range2517156
display id: range2517156
version: 1
direction: direction/forward
top level: VP64
start: 217
end: 240
orientation: inline

VP16
type: SequenceAnnotation
persistent identity: annotation2517153
display id: annotation2517153
version: 1
top level: VP64
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1982012/annotation2517153/range2517153/1

https://synbiohub.org/public/igem/BBa_K1982012/annotation2517153/range2517153/1
type: Range
persistent identity: range2517153
display id: range2517153
version: 1
direction: direction/forward
top level: VP64
start: 142
end: 180
orientation: inline

SV40 NLS
type: SequenceAnnotation
persistent identity: annotation2517149
display id: annotation2517149
version: 1
top level: VP64
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1982012/annotation2517149/range2517149/1

https://synbiohub.org/public/igem/BBa_K1982012/annotation2517149/range2517149/1
type: Range
persistent identity: range2517149
display id: range2517149
version: 1
direction: direction/forward
top level: VP64
start: 1
end: 21
orientation: inline

VP16
type: SequenceAnnotation
persistent identity: annotation2517152
display id: annotation2517152
version: 1
top level: VP64
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1982012/annotation2517152/range2517152/1

https://synbiohub.org/public/igem/BBa_K1982012/annotation2517152/range2517152/1
type: Range
persistent identity: range2517152
display id: range2517152
version: 1
direction: direction/forward
top level: VP64
start: 103
end: 141
orientation: inline

HA
type: SequenceAnnotation
persistent identity: annotation2517155
display id: annotation2517155
version: 1
top level: VP64
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1982012/annotation2517155/range2517155/1

https://synbiohub.org/public/igem/BBa_K1982012/annotation2517155/range2517155/1
type: Range
persistent identity: range2517155
display id: range2517155
version: 1
direction: direction/forward
top level: VP64
start: 187
end: 213
orientation: inline

TAATAA
type: SequenceAnnotation
persistent identity: annotation2517157
display id: annotation2517157
version: 1
top level: VP64
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1982012/annotation2517157/range2517157/1

https://synbiohub.org/public/igem/BBa_K1982012/annotation2517157/range2517157/1
type: Range
persistent identity: range2517157
display id: range2517157
version: 1
direction: direction/forward
top level: VP64
start: 241
end: 246
orientation: inline

VP16
type: SequenceAnnotation
persistent identity: annotation2517151
display id: annotation2517151
version: 1
top level: VP64
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1982012/annotation2517151/range2517151/1

https://synbiohub.org/public/igem/BBa_K1982012/annotation2517151/range2517151/1
type: Range
persistent identity: range2517151
display id: range2517151
version: 1
direction: direction/forward
top level: VP64
start: 64
end: 102
orientation: inline

VP16
type: SequenceAnnotation
persistent identity: annotation2517150
display id: annotation2517150
version: 1
top level: VP64
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1982012/annotation2517150/range2517150/1

https://synbiohub.org/public/igem/BBa_K1982012/annotation2517150/range2517150/1
type: Range
persistent identity: range2517150
display id: range2517150
version: 1
direction: direction/forward
top level: VP64
start: 22
end: 63
orientation: inline

Linker
type: SequenceAnnotation
persistent identity: annotation2517154
display id: annotation2517154
version: 1
top level: VP64
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1982012/annotation2517154/range2517154/1

https://synbiohub.org/public/igem/BBa_K1982012/annotation2517154/range2517154/1
type: Range
persistent identity: range2517154
display id: range2517154
version: 1
direction: direction/forward
top level: VP64
start: 181
end: 186
orientation: inline

BBa_K1926031
type: ComponentDefinition
persistent identity: BBa K1926031
display id: BBa_K1926031
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: The SNAP-mCHERRY- CYAN UNIT
creator: Xinyi Liu
created: 2016-10-09T11:00:00Z
modified: 2016-10-11T07:02:32Z
discontinued: false
dominant: false
group u list: _2393_
m user id: 28998
owner id: 28998
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This part is the SNAP-mCHERRY-CYAN UNIT of cyclebow including a SNAP UNIT, a mCHERRY UNIT and a plain CYAN. The SNAP UNIT, which can be cut off by CRE, includes an sv40 nuclear location sequence (NLS), a covalent labeling of fusion proteins named SNAP-tag?? and an sv40 terminator flanked by homodromous recognition site of recombinase CRE named loxP. The mCHERRY UNIT, which can be cut off by VIKA, includes a degradation tag named DBOX, a fluorescent gene mCherry and an sv40 terminator flanked by homodromous recognition site of recombinase VIKA named Vox. You may use this part to: 1) Test the cut-off efficiency of recombinase CRE and VIKA; 2) Estimate the time needed for genome repair because mCherry can only be expressed after the cut off of SNAP UNIT and genome repair; 3) Fast cut off this part by transient transfecting recombinase gene into nucleus; 4) When there is no CRE, you can use this part as the SNAP UNIT, which can help localize proteins in vivo with BLOCK and dye from NEB company.
mutable notes: None
mutable provenance: The SNAP-tag?? was brought from NEB company. NLS and sv40 terminator were got from commercial plasmid: pentry nls GFP/pcdna3.1. The sequence of Vox and DBOX was retrieved from Addgene and got through denovo synthesis. mCHERRY was got from commercial plasmid: pentry dcas9 mcherry bira. CYAN was got from commercial plasmid: puc18 cfp-yfp.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: CYAN
sequence annotation: mutate C into A to remove Xba1
sequence annotation: BBa_K1926022
sequence: https://synbiohub.org/public/igem/BBa_K1926031_sequence/1

CYAN
type: SequenceAnnotation
persistent identity: annotation2491946
display id: annotation2491946
version: 1
top level: BBa_K1926031
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1926031/annotation2491946/range2491946/1

https://synbiohub.org/public/igem/BBa_K1926031/annotation2491946/range2491946/1
type: Range
persistent identity: range2491946
display id: range2491946
version: 1
direction: direction/forward
top level: BBa_K1926031
start: 1712
end: 2428
orientation: inline

mutate C into A to remove Xba1
type: SequenceAnnotation
persistent identity: annotation2491947
display id: annotation2491947
version: 1
top level: BBa_K1926031
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K1926031/annotation2491947/range2491947/1

https://synbiohub.org/public/igem/BBa_K1926031/annotation2491947/range2491947/1
type: Range
persistent identity: range2491947
display id: range2491947
version: 1
direction: direction/forward
top level: BBa_K1926031
start: 1707
end: 1707
orientation: inline

BBa_K1926022
type: SequenceAnnotation
persistent identity: annotation2489581
display id: annotation2489581
version: 1
top level: BBa_K1926031
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1926031/annotation2489581/range2489581/1

https://synbiohub.org/public/igem/BBa_K1926031/annotation2489581/range2489581/1
type: Range
persistent identity: range2489581
display id: range2489581
version: 1
direction: direction/forward
top level: BBa_K1926031
start: 1
end: 1704
orientation: inline

BBa_K1926013
type: ComponentDefinition
persistent identity: BBa K1926013
display id: BBa_K1926013
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: The mCHERRY UNIT: mCherry flanked by Vox
creator: Xinyi Liu
created: 2016-10-08T11:00:00Z
modified: 2016-10-11T05:50:50Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2393_
m user id: 28998
owner id: 28998
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This part is the mCHERRY UNIT of cyclebow system including a degradation tag named DBOX, a fluorescent gene mCherry and an sv40 terminator flanked by homodromous recognition site of recombinase VIKA named Vox. The whole part can be cut off by VIKA. You may use this part to: 1) Provide fluorescent red which can be quickly cut off by transient transfecting recombinase gene into nucleus; 2) Use it as a part of the flp-out system[5]. 5. Voutev, R. and E.J. Hubbard, A "FLP-Out" system for controlled gene expression in Caenorhabditis elegans. Genetics, 2008. 180(1): p. 103-19.
mutable notes: None
mutable provenance: The sequence of Vox and DBOX was retrieved from Addgene and got through denovo synthesis. mCHERRY and sv40 terminator were got from commercial plasmid: pentry dcas9 mcherry bira and pentry nls GFP/pcdna3.1, respectfully.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: mutate C into A to remove Pst1
sequence annotation: sv40 terminator
sequence annotation: vox
sequence annotation: mCherry
sequence annotation: TAA
sequence: https://synbiohub.org/public/igem/BBa_K1926013_sequence/1

mutate C into A to remove Pst1
type: SequenceAnnotation
persistent identity: annotation2491882
display id: annotation2491882
version: 1
top level: BBa_K1926013
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K1926013/annotation2491882/range2491882/1

https://synbiohub.org/public/igem/BBa_K1926013/annotation2491882/range2491882/1
type: Range
persistent identity: range2491882
display id: range2491882
version: 1
direction: direction/forward
top level: BBa_K1926013
start: 384
end: 384
orientation: inline

sv40 terminator
type: SequenceAnnotation
persistent identity: annotation2491888
display id: annotation2491888
version: 1
top level: BBa_K1926013
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1926013/annotation2491888/range2491888/1

https://synbiohub.org/public/igem/BBa_K1926013/annotation2491888/range2491888/1
type: Range
persistent identity: range2491888
display id: range2491888
version: 1
direction: direction/forward
top level: BBa_K1926013
start: 740
end: 882
orientation: inline

vox
type: SequenceAnnotation
persistent identity: annotation2491874
display id: annotation2491874
version: 1
top level: BBa_K1926013
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1926013/annotation2491874/range2491874/1

https://synbiohub.org/public/igem/BBa_K1926013/annotation2491874/range2491874/1
type: Range
persistent identity: range2491874
display id: range2491874
version: 1
direction: direction/forward
top level: BBa_K1926013
start: 1
end: 34
orientation: inline

mCherry
type: SequenceAnnotation
persistent identity: annotation2491876
display id: annotation2491876
version: 1
top level: BBa_K1926013
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1926013/annotation2491876/range2491876/1

https://synbiohub.org/public/igem/BBa_K1926013/annotation2491876/range2491876/1
type: Range
persistent identity: range2491876
display id: range2491876
version: 1
direction: direction/forward
top level: BBa_K1926013
start: 35
end: 739
orientation: inline

vox
type: SequenceAnnotation
persistent identity: annotation2491890
display id: annotation2491890
version: 1
top level: BBa_K1926013
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1926013/annotation2491890/range2491890/1

https://synbiohub.org/public/igem/BBa_K1926013/annotation2491890/range2491890/1
type: Range
persistent identity: range2491890
display id: range2491890
version: 1
direction: direction/forward
top level: BBa_K1926013
start: 883
end: 916
orientation: inline

TAA
type: SequenceAnnotation
persistent identity: annotation2491891
display id: annotation2491891
version: 1
top level: BBa_K1926013
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1926013/annotation2491891/range2491891/1

https://synbiohub.org/public/igem/BBa_K1926013/annotation2491891/range2491891/1
type: Range
persistent identity: range2491891
display id: range2491891
version: 1
direction: direction/forward
top level: BBa_K1926013
start: 759
end: 761
orientation: inline

BBa_K404125
type: ComponentDefinition
persistent identity: BBa K404125
display id: BBa_K404125
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: [AAV2]-left-ITR_phTERT_betaglobin_CD_hGH_[AAV2]-right-ITR
creator: Freiburg Bioware 2010
created: 2010-10-10T11:00:00Z
modified: 2015-05-08T01:12:23Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 6733
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: Beta-Globin-intron
sequence annotation: right ITR
sequence annotation: cytosine deaminase
sequence annotation: hGH terminator
sequence annotation: left ITR
sequence annotation: phTERT
sequence: https://synbiohub.org/public/igem/BBa_K404125_sequence/1

Beta-Globin-intron
type: SequenceAnnotation
persistent identity: annotation2093284
display id: annotation2093284
version: 1
top level: BBa_K404125
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404125/annotation2093284/range2093284/1

https://synbiohub.org/public/igem/BBa_K404125/annotation2093284/range2093284/1
type: Range
persistent identity: range2093284
display id: range2093284
version: 1
direction: direction/forward
top level: BBa_K404125
start: 619
end: 1113
orientation: inline

right ITR
type: SequenceAnnotation
persistent identity: annotation2093287
display id: annotation2093287
version: 1
top level: BBa_K404125
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404125/annotation2093287/range2093287/1

https://synbiohub.org/public/igem/BBa_K404125/annotation2093287/range2093287/1
type: Range
persistent identity: range2093287
display id: range2093287
version: 1
direction: direction/forward
top level: BBa_K404125
start: 2919
end: 3056
orientation: inline

cytosine deaminase
type: SequenceAnnotation
persistent identity: annotation2093285
display id: annotation2093285
version: 1
top level: BBa_K404125
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404125/annotation2093285/range2093285/1

https://synbiohub.org/public/igem/BBa_K404125/annotation2093285/range2093285/1
type: Range
persistent identity: range2093285
display id: range2093285
version: 1
direction: direction/forward
top level: BBa_K404125
start: 1128
end: 2408
orientation: inline

hGH terminator
type: SequenceAnnotation
persistent identity: annotation2093289
display id: annotation2093289
version: 1
top level: BBa_K404125
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404125/annotation2093289/range2093289/1

https://synbiohub.org/public/igem/BBa_K404125/annotation2093289/range2093289/1
type: Range
persistent identity: range2093289
display id: range2093289
version: 1
direction: direction/forward
top level: BBa_K404125
start: 2425
end: 2904
orientation: inline

left ITR
type: SequenceAnnotation
persistent identity: annotation2093281
display id: annotation2093281
version: 1
top level: BBa_K404125
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404125/annotation2093281/range2093281/1

https://synbiohub.org/public/igem/BBa_K404125/annotation2093281/range2093281/1
type: Range
persistent identity: range2093281
display id: range2093281
version: 1
direction: direction/forward
top level: BBa_K404125
start: 1
end: 147
orientation: inline

phTERT
type: SequenceAnnotation
persistent identity: annotation2093282
display id: annotation2093282
version: 1
top level: BBa_K404125
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K404125/annotation2093282/range2093282/1

https://synbiohub.org/public/igem/BBa_K404125/annotation2093282/range2093282/1
type: Range
persistent identity: range2093282
display id: range2093282
version: 1
direction: direction/forward
top level: BBa_K404125
start: 154
end: 612
orientation: inline

BBa_K404163
type: ComponentDefinition
persistent identity: BBa K404163
display id: BBa_K404163
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pCMV_Z-EGFR-1907_Middle-Linker_[AAV2]-VP23 (ViralBrick-587KO-His-Tag)
creator: Freiburg Bioware 2010
created: 2010-10-11T11:00:00Z
modified: 2015-05-08T01:12:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 6733
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: ZEGFR_1907
sequence annotation: downstream 587
sequence annotation: A20-2 binding site
sequence annotation: basic region 2
sequence annotation: TG-spacer
sequence annotation: scar
sequence annotation: basic region 3
sequence annotation: VP2
sequence annotation: HSPG-KO
sequence annotation: middle-linker
sequence annotation: VP3
sequence annotation: His-tag
sequence annotation: B1 binding site
sequence annotation: GLS-spacer
sequence annotation: A20-4 binding site
sequence annotation: basic region 4
sequence annotation: upstream 587
sequence annotation: AAV-2-cap (Stratagene)
sequence annotation: A20-1 binding site
sequence annotation: CMV
sequence: https://synbiohub.org/public/igem/BBa_K404163_sequence/1

ZEGFR_1907
type: SequenceAnnotation
persistent identity: annotation2101690
display id: annotation2101690
version: 1
top level: BBa_K404163
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K404163/annotation2101690/range2101690/1

https://synbiohub.org/public/igem/BBa_K404163/annotation2101690/range2101690/1
type: Range
persistent identity: range2101690
display id: range2101690
version: 1
direction: direction/forward
top level: BBa_K404163
start: 671
end: 844
orientation: inline

downstream 587
type: SequenceAnnotation
persistent identity: annotation2101708
display id: annotation2101708
version: 1
top level: BBa_K404163
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404163/annotation2101708/range2101708/1

https://synbiohub.org/public/igem/BBa_K404163/annotation2101708/range2101708/1
type: Range
persistent identity: range2101708
display id: range2101708
version: 1
direction: direction/forward
top level: BBa_K404163
start: 2261
end: 2276
orientation: inline

A20-2 binding site
type: SequenceAnnotation
persistent identity: annotation2101701
display id: annotation2101701
version: 1
top level: BBa_K404163
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K404163/annotation2101701/range2101701/1

https://synbiohub.org/public/igem/BBa_K404163/annotation2101701/range2101701/1
type: Range
persistent identity: range2101701
display id: range2101701
version: 1
direction: direction/forward
top level: BBa_K404163
start: 1574
end: 1600
orientation: inline

basic region 2
type: SequenceAnnotation
persistent identity: annotation2101696
display id: annotation2101696
version: 1
top level: BBa_K404163
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404163/annotation2101696/range2101696/1

https://synbiohub.org/public/igem/BBa_K404163/annotation2101696/range2101696/1
type: Range
persistent identity: range2101696
display id: range2101696
version: 1
direction: direction/forward
top level: BBa_K404163
start: 884
end: 904
orientation: inline

TG-spacer
type: SequenceAnnotation
persistent identity: annotation2101705
display id: annotation2101705
version: 1
top level: BBa_K404163
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404163/annotation2101705/range2101705/1

https://synbiohub.org/public/igem/BBa_K404163/annotation2101705/range2101705/1
type: Range
persistent identity: range2101705
display id: range2101705
version: 1
direction: direction/forward
top level: BBa_K404163
start: 2228
end: 2233
orientation: inline

scar
type: SequenceAnnotation
persistent identity: annotation2101691
display id: annotation2101691
version: 1
top level: BBa_K404163
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404163/annotation2101691/range2101691/1

https://synbiohub.org/public/igem/BBa_K404163/annotation2101691/range2101691/1
type: Range
persistent identity: range2101691
display id: range2101691
version: 1
direction: direction/forward
top level: BBa_K404163
start: 845
end: 850
orientation: inline

basic region 3
type: SequenceAnnotation
persistent identity: annotation2101697
display id: annotation2101697
version: 1
top level: BBa_K404163
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404163/annotation2101697/range2101697/1

https://synbiohub.org/public/igem/BBa_K404163/annotation2101697/range2101697/1
type: Range
persistent identity: range2101697
display id: range2101697
version: 1
direction: direction/forward
top level: BBa_K404163
start: 962
end: 982
orientation: inline

VP2
type: SequenceAnnotation
persistent identity: annotation2101694
display id: annotation2101694
version: 1
top level: BBa_K404163
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K404163/annotation2101694/range2101694/1

https://synbiohub.org/public/igem/BBa_K404163/annotation2101694/range2101694/1
type: Range
persistent identity: range2101694
display id: range2101694
version: 1
direction: direction/forward
top level: BBa_K404163
start: 881
end: 2707
orientation: inline

HSPG-KO
type: SequenceAnnotation
persistent identity: annotation2101704
display id: annotation2101704
version: 1
top level: BBa_K404163
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K404163/annotation2101704/range2101704/1

https://synbiohub.org/public/igem/BBa_K404163/annotation2101704/range2101704/1
type: Range
persistent identity: range2101704
display id: range2101704
version: 1
direction: direction/forward
top level: BBa_K404163
start: 2219
end: 2221
orientation: inline

scar
type: SequenceAnnotation
persistent identity: annotation2101689
display id: annotation2101689
version: 1
top level: BBa_K404163
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404163/annotation2101689/range2101689/1

https://synbiohub.org/public/igem/BBa_K404163/annotation2101689/range2101689/1
type: Range
persistent identity: range2101689
display id: range2101689
version: 1
direction: direction/forward
top level: BBa_K404163
start: 656
end: 670
orientation: inline

middle-linker
type: SequenceAnnotation
persistent identity: annotation2101692
display id: annotation2101692
version: 1
top level: BBa_K404163
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404163/annotation2101692/range2101692/1

https://synbiohub.org/public/igem/BBa_K404163/annotation2101692/range2101692/1
type: Range
persistent identity: range2101692
display id: range2101692
version: 1
direction: direction/forward
top level: BBa_K404163
start: 851
end: 874
orientation: inline

VP3
type: SequenceAnnotation
persistent identity: annotation2101698
display id: annotation2101698
version: 1
top level: BBa_K404163
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K404163/annotation2101698/range2101698/1

https://synbiohub.org/public/igem/BBa_K404163/annotation2101698/range2101698/1
type: Range
persistent identity: range2101698
display id: range2101698
version: 1
direction: direction/forward
top level: BBa_K404163
start: 1073
end: 2707
orientation: inline

His-tag
type: SequenceAnnotation
persistent identity: annotation2101706
display id: annotation2101706
version: 1
top level: BBa_K404163
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404163/annotation2101706/range2101706/1

https://synbiohub.org/public/igem/BBa_K404163/annotation2101706/range2101706/1
type: Range
persistent identity: range2101706
display id: range2101706
version: 1
direction: direction/forward
top level: BBa_K404163
start: 2234
end: 2251
orientation: inline

B1 binding site
type: SequenceAnnotation
persistent identity: annotation2101710
display id: annotation2101710
version: 1
top level: BBa_K404163
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404163/annotation2101710/range2101710/1

https://synbiohub.org/public/igem/BBa_K404163/annotation2101710/range2101710/1
type: Range
persistent identity: range2101710
display id: range2101710
version: 1
direction: direction/forward
top level: BBa_K404163
start: 2675
end: 2698
orientation: inline

HSPG-KO
type: SequenceAnnotation
persistent identity: annotation2101709
display id: annotation2101709
version: 1
top level: BBa_K404163
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K404163/annotation2101709/range2101709/1

https://synbiohub.org/public/igem/BBa_K404163/annotation2101709/range2101709/1
type: Range
persistent identity: range2101709
display id: range2101709
version: 1
direction: direction/forward
top level: BBa_K404163
start: 2288
end: 2290
orientation: inline

GLS-spacer
type: SequenceAnnotation
persistent identity: annotation2101707
display id: annotation2101707
version: 1
top level: BBa_K404163
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404163/annotation2101707/range2101707/1

https://synbiohub.org/public/igem/BBa_K404163/annotation2101707/range2101707/1
type: Range
persistent identity: range2101707
display id: range2101707
version: 1
direction: direction/forward
top level: BBa_K404163
start: 2252
end: 2260
orientation: inline

A20-4 binding site
type: SequenceAnnotation
persistent identity: annotation2101702
display id: annotation2101702
version: 1
top level: BBa_K404163
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K404163/annotation2101702/range2101702/1

https://synbiohub.org/public/igem/BBa_K404163/annotation2101702/range2101702/1
type: Range
persistent identity: range2101702
display id: range2101702
version: 1
direction: direction/forward
top level: BBa_K404163
start: 2162
end: 2191
orientation: inline

scar
type: SequenceAnnotation
persistent identity: annotation2101693
display id: annotation2101693
version: 1
top level: BBa_K404163
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404163/annotation2101693/range2101693/1

https://synbiohub.org/public/igem/BBa_K404163/annotation2101693/range2101693/1
type: Range
persistent identity: range2101693
display id: range2101693
version: 1
direction: direction/forward
top level: BBa_K404163
start: 875
end: 880
orientation: inline

basic region 4
type: SequenceAnnotation
persistent identity: annotation2101700
display id: annotation2101700
version: 1
top level: BBa_K404163
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404163/annotation2101700/range2101700/1

https://synbiohub.org/public/igem/BBa_K404163/annotation2101700/range2101700/1
type: Range
persistent identity: range2101700
display id: range2101700
version: 1
direction: direction/forward
top level: BBa_K404163
start: 1385
end: 1402
orientation: inline

upstream 587
type: SequenceAnnotation
persistent identity: annotation2101703
display id: annotation2101703
version: 1
top level: BBa_K404163
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404163/annotation2101703/range2101703/1

https://synbiohub.org/public/igem/BBa_K404163/annotation2101703/range2101703/1
type: Range
persistent identity: range2101703
display id: range2101703
version: 1
direction: direction/forward
top level: BBa_K404163
start: 2196
end: 2227
orientation: inline

AAV-2-cap (Stratagene)
type: SequenceAnnotation
persistent identity: annotation2101695
display id: annotation2101695
version: 1
top level: BBa_K404163
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K404163/annotation2101695/range2101695/1

https://synbiohub.org/public/igem/BBa_K404163/annotation2101695/range2101695/1
type: Range
persistent identity: range2101695
display id: range2101695
version: 1
direction: direction/forward
top level: BBa_K404163
start: 881
end: 2830
orientation: inline

A20-1 binding site
type: SequenceAnnotation
persistent identity: annotation2101699
display id: annotation2101699
version: 1
top level: BBa_K404163
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K404163/annotation2101699/range2101699/1

https://synbiohub.org/public/igem/BBa_K404163/annotation2101699/range2101699/1
type: Range
persistent identity: range2101699
display id: range2101699
version: 1
direction: direction/forward
top level: BBa_K404163
start: 1277
end: 1306
orientation: inline

CMV
type: SequenceAnnotation
persistent identity: annotation2101688
display id: annotation2101688
version: 1
top level: BBa_K404163
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K404163/annotation2101688/range2101688/1

https://synbiohub.org/public/igem/BBa_K404163/annotation2101688/range2101688/1
type: Range
persistent identity: range2101688
display id: range2101688
version: 1
direction: direction/forward
top level: BBa_K404163
start: 2
end: 655
orientation: inline

BBa_K1926011
type: ComponentDefinition
persistent identity: BBa K1926011
display id: BBa_K1926011
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: The SNAP UNIT: SNAP-tag flanked by loxP
creator: Xinyi Liu
created: 2016-10-08T11:00:00Z
modified: 2016-10-19T01:22:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2393_
m user id: 28998
owner id: 28998
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This part is the SNAP UNIT of cyclebow system including an sv40 nuclear location sequence (NLS), a covalent labeling of fusion proteins named SNAP-tag?? and an sv40 terminator flanked by homodromous recognition site of recombinase CRE named loxP. The whole part can be cut off by CRE. You may use this part to: 1) Localize proteins in vivo with BLOCK and dye from NEB company; 2) Dye after blocking, you are able to see whether an event have an influence on the localization and expression of the interested protein; 3) Fast cut off this part by transient transfecting recombinase gene into nucleus.
mutable notes: None
mutable provenance: The SNAP-tag?? was brought from NEB company. NLS and sv40 terminator were got from commercial plasmid: pentry nls GFP/pcdna3.1.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: SNAP-tag
sequence annotation: TAA
sequence annotation: sv40 terminator
sequence annotation: loxP
sequence annotation: NLS
sequence: https://synbiohub.org/public/igem/BBa_K1926011_sequence/1

SNAP-tag
type: SequenceAnnotation
persistent identity: annotation2491738
display id: annotation2491738
version: 1
top level: BBa_K1926011
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1926011/annotation2491738/range2491738/1

https://synbiohub.org/public/igem/BBa_K1926011/annotation2491738/range2491738/1
type: Range
persistent identity: range2491738
display id: range2491738
version: 1
direction: direction/forward
top level: BBa_K1926011
start: 59
end: 604
orientation: inline

TAA
type: SequenceAnnotation
persistent identity: annotation2491851
display id: annotation2491851
version: 1
top level: BBa_K1926011
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1926011/annotation2491851/range2491851/1

https://synbiohub.org/public/igem/BBa_K1926011/annotation2491851/range2491851/1
type: Range
persistent identity: range2491851
display id: range2491851
version: 1
direction: direction/forward
top level: BBa_K1926011
start: 626
end: 628
orientation: inline

sv40 terminator
type: SequenceAnnotation
persistent identity: annotation2491779
display id: annotation2491779
version: 1
top level: BBa_K1926011
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1926011/annotation2491779/range2491779/1

https://synbiohub.org/public/igem/BBa_K1926011/annotation2491779/range2491779/1
type: Range
persistent identity: range2491779
display id: range2491779
version: 1
direction: direction/forward
top level: BBa_K1926011
start: 605
end: 754
orientation: inline

loxP
type: SequenceAnnotation
persistent identity: annotation2491774
display id: annotation2491774
version: 1
top level: BBa_K1926011
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1926011/annotation2491774/range2491774/1

https://synbiohub.org/public/igem/BBa_K1926011/annotation2491774/range2491774/1
type: Range
persistent identity: range2491774
display id: range2491774
version: 1
direction: direction/forward
top level: BBa_K1926011
start: 1
end: 34
orientation: inline

loxP
type: SequenceAnnotation
persistent identity: annotation2491776
display id: annotation2491776
version: 1
top level: BBa_K1926011
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1926011/annotation2491776/range2491776/1

https://synbiohub.org/public/igem/BBa_K1926011/annotation2491776/range2491776/1
type: Range
persistent identity: range2491776
display id: range2491776
version: 1
direction: direction/forward
top level: BBa_K1926011
start: 755
end: 788
orientation: inline

NLS
type: SequenceAnnotation
persistent identity: annotation2491778
display id: annotation2491778
version: 1
top level: BBa_K1926011
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1926011/annotation2491778/range2491778/1

https://synbiohub.org/public/igem/BBa_K1926011/annotation2491778/range2491778/1
type: Range
persistent identity: range2491778
display id: range2491778
version: 1
direction: direction/forward
top level: BBa_K1926011
start: 38
end: 58
orientation: inline

BBa_K404115
type: ComponentDefinition
persistent identity: BBa K404115
display id: BBa_K404115
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: [AAV2]-left-ITR_phTERT
creator: Freiburg Bioware 2010
created: 2010-10-10T11:00:00Z
modified: 2015-05-08T01:12:23Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 6733
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: phTERT
sequence annotation: left ITR
sequence: https://synbiohub.org/public/igem/BBa_K404115_sequence/1

phTERT
type: SequenceAnnotation
persistent identity: annotation2093186
display id: annotation2093186
version: 1
top level: BBa_K404115
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K404115/annotation2093186/range2093186/1

https://synbiohub.org/public/igem/BBa_K404115/annotation2093186/range2093186/1
type: Range
persistent identity: range2093186
display id: range2093186
version: 1
direction: direction/forward
top level: BBa_K404115
start: 151
end: 612
orientation: inline

left ITR
type: SequenceAnnotation
persistent identity: annotation2093185
display id: annotation2093185
version: 1
top level: BBa_K404115
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404115/annotation2093185/range2093185/1

https://synbiohub.org/public/igem/BBa_K404115/annotation2093185/range2093185/1
type: Range
persistent identity: range2093185
display id: range2093185
version: 1
direction: direction/forward
top level: BBa_K404115
start: 1
end: 147
orientation: inline

BBa_K404008
type: ComponentDefinition
persistent identity: BBa K404008
display id: BBa_K404008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: [AAV2]-VP1
creator: Freiburg Bioware 2010
created: 2010-10-20T11:00:00Z
modified: 2015-05-08T01:12:23Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 6674
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: VP1
sequence: https://synbiohub.org/public/igem/BBa_K404008_sequence/1

VP1
type: SequenceAnnotation
persistent identity: annotation2107872
display id: annotation2107872
version: 1
top level: BBa_K404008
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404008/annotation2107872/range2107872/1

https://synbiohub.org/public/igem/BBa_K404008/annotation2107872/range2107872/1
type: Range
persistent identity: range2107872
display id: range2107872
version: 1
direction: direction/forward
top level: BBa_K404008
start: 1
end: 2202
orientation: inline

BBa_K404168
type: ComponentDefinition
persistent identity: BBa K404168
display id: BBa_K404168
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pCMV_VP1up_NLS_mVenus_VP2/3(ViralBrick-587-KO-His)
creator: Freiburg Bioware 2010
created: 2010-10-23T11:00:00Z
modified: 2015-05-08T01:12:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 6674
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: TG-spacer
sequence annotation: downstream 587
sequence annotation: NLS
sequence annotation: mVenus
sequence annotation: GLS-spacer
sequence annotation: AAV-2-cap (Stratagene)
sequence annotation: upstream 587
sequence annotation: VP1up
sequence annotation: HSPG-KO
sequence annotation: VP2
sequence annotation: CMV
sequence annotation: VP3
sequence annotation: His-tag
sequence: https://synbiohub.org/public/igem/BBa_K404168_sequence/1

TG-spacer
type: SequenceAnnotation
persistent identity: annotation2095288
display id: annotation2095288
version: 1
top level: BBa_K404168
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404168/annotation2095288/range2095288/1

https://synbiohub.org/public/igem/BBa_K404168/annotation2095288/range2095288/1
type: Range
persistent identity: range2095288
display id: range2095288
version: 1
direction: direction/forward
top level: BBa_K404168
start: 3182
end: 3187
orientation: inline

downstream 587
type: SequenceAnnotation
persistent identity: annotation2095292
display id: annotation2095292
version: 1
top level: BBa_K404168
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404168/annotation2095292/range2095292/1

https://synbiohub.org/public/igem/BBa_K404168/annotation2095292/range2095292/1
type: Range
persistent identity: range2095292
display id: range2095292
version: 1
direction: direction/forward
top level: BBa_K404168
start: 3215
end: 3230
orientation: inline

NLS
type: SequenceAnnotation
persistent identity: annotation2095284
display id: annotation2095284
version: 1
top level: BBa_K404168
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404168/annotation2095284/range2095284/1

https://synbiohub.org/public/igem/BBa_K404168/annotation2095284/range2095284/1
type: Range
persistent identity: range2095284
display id: range2095284
version: 1
direction: direction/forward
top level: BBa_K404168
start: 1088
end: 1108
orientation: inline

mVenus
type: SequenceAnnotation
persistent identity: annotation2095282
display id: annotation2095282
version: 1
top level: BBa_K404168
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404168/annotation2095282/range2095282/1

https://synbiohub.org/public/igem/BBa_K404168/annotation2095282/range2095282/1
type: Range
persistent identity: range2095282
display id: range2095282
version: 1
direction: direction/forward
top level: BBa_K404168
start: 1115
end: 1828
orientation: inline

GLS-spacer
type: SequenceAnnotation
persistent identity: annotation2095290
display id: annotation2095290
version: 1
top level: BBa_K404168
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404168/annotation2095290/range2095290/1

https://synbiohub.org/public/igem/BBa_K404168/annotation2095290/range2095290/1
type: Range
persistent identity: range2095290
display id: range2095290
version: 1
direction: direction/forward
top level: BBa_K404168
start: 3206
end: 3214
orientation: inline

AAV-2-cap (Stratagene)
type: SequenceAnnotation
persistent identity: annotation2095294
display id: annotation2095294
version: 1
top level: BBa_K404168
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404168/annotation2095294/range2095294/1

https://synbiohub.org/public/igem/BBa_K404168/annotation2095294/range2095294/1
type: Range
persistent identity: range2095294
display id: range2095294
version: 1
direction: direction/forward
top level: BBa_K404168
start: 1835
end: 3784
orientation: inline

upstream 587
type: SequenceAnnotation
persistent identity: annotation2095293
display id: annotation2095293
version: 1
top level: BBa_K404168
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404168/annotation2095293/range2095293/1

https://synbiohub.org/public/igem/BBa_K404168/annotation2095293/range2095293/1
type: Range
persistent identity: range2095293
display id: range2095293
version: 1
direction: direction/forward
top level: BBa_K404168
start: 3150
end: 3181
orientation: inline

VP1up
type: SequenceAnnotation
persistent identity: annotation2095281
display id: annotation2095281
version: 1
top level: BBa_K404168
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404168/annotation2095281/range2095281/1

https://synbiohub.org/public/igem/BBa_K404168/annotation2095281/range2095281/1
type: Range
persistent identity: range2095281
display id: range2095281
version: 1
direction: direction/forward
top level: BBa_K404168
start: 673
end: 1081
orientation: inline

HSPG-KO
type: SequenceAnnotation
persistent identity: annotation2101845
display id: annotation2101845
version: 1
top level: BBa_K404168
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K404168/annotation2101845/range2101845/1

https://synbiohub.org/public/igem/BBa_K404168/annotation2101845/range2101845/1
type: Range
persistent identity: range2101845
display id: range2101845
version: 1
direction: direction/forward
top level: BBa_K404168
start: 3163
end: 3165
orientation: inline

VP2
type: SequenceAnnotation
persistent identity: annotation2095285
display id: annotation2095285
version: 1
top level: BBa_K404168
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404168/annotation2095285/range2095285/1

https://synbiohub.org/public/igem/BBa_K404168/annotation2095285/range2095285/1
type: Range
persistent identity: range2095285
display id: range2095285
version: 1
direction: direction/forward
top level: BBa_K404168
start: 1835
end: 3661
orientation: inline

HSPG-KO
type: SequenceAnnotation
persistent identity: annotation2101844
display id: annotation2101844
version: 1
top level: BBa_K404168
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K404168/annotation2101844/range2101844/1

https://synbiohub.org/public/igem/BBa_K404168/annotation2101844/range2101844/1
type: Range
persistent identity: range2101844
display id: range2101844
version: 1
direction: direction/forward
top level: BBa_K404168
start: 3215
end: 3217
orientation: inline

CMV
type: SequenceAnnotation
persistent identity: annotation2095279
display id: annotation2095279
version: 1
top level: BBa_K404168
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K404168/annotation2095279/range2095279/1

https://synbiohub.org/public/igem/BBa_K404168/annotation2095279/range2095279/1
type: Range
persistent identity: range2095279
display id: range2095279
version: 1
direction: direction/forward
top level: BBa_K404168
start: 2
end: 655
orientation: inline

VP3
type: SequenceAnnotation
persistent identity: annotation2095286
display id: annotation2095286
version: 1
top level: BBa_K404168
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404168/annotation2095286/range2095286/1

https://synbiohub.org/public/igem/BBa_K404168/annotation2095286/range2095286/1
type: Range
persistent identity: range2095286
display id: range2095286
version: 1
direction: direction/forward
top level: BBa_K404168
start: 2027
end: 3661
orientation: inline

His-tag
type: SequenceAnnotation
persistent identity: annotation2095287
display id: annotation2095287
version: 1
top level: BBa_K404168
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404168/annotation2095287/range2095287/1

https://synbiohub.org/public/igem/BBa_K404168/annotation2095287/range2095287/1
type: Range
persistent identity: range2095287
display id: range2095287
version: 1
direction: direction/forward
top level: BBa_K404168
start: 3188
end: 3205
orientation: inline

BBa_K404210
type: ComponentDefinition
persistent identity: BBa K404210
display id: BBa_K404210
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: ViralBrick-587KO-Empty
creator: Freiburg Bioware 2010
created: 2010-08-27T11:00:00Z
modified: 2015-05-08T01:12:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 7049
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: downstream 587
sequence annotation: upstream 453
sequence annotation: R=>A
sequence annotation: downstream 453
sequence annotation: upstream 587
sequence: https://synbiohub.org/public/igem/BBa_K404210_sequence/1

downstream 587
type: SequenceAnnotation
persistent identity: annotation2079127
display id: annotation2079127
version: 1
top level: BBa_K404210
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404210/annotation2079127/range2079127/1

https://synbiohub.org/public/igem/BBa_K404210/annotation2079127/range2079127/1
type: Range
persistent identity: range2079127
display id: range2079127
version: 1
direction: direction/forward
top level: BBa_K404210
start: 43
end: 63
orientation: inline

upstream 453
type: SequenceAnnotation
persistent identity: annotation2079125
display id: annotation2079125
version: 1
top level: BBa_K404210
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404210/annotation2079125/range2079125/1

https://synbiohub.org/public/igem/BBa_K404210/annotation2079125/range2079125/1
type: Range
persistent identity: range2079125
display id: range2079125
version: 1
direction: direction/forward
top level: BBa_K404210
start: 1
end: 9
orientation: inline

R=>A
type: SequenceAnnotation
persistent identity: annotation2079130
display id: annotation2079130
version: 1
top level: BBa_K404210
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K404210/annotation2079130/range2079130/1

https://synbiohub.org/public/igem/BBa_K404210/annotation2079130/range2079130/1
type: Range
persistent identity: range2079130
display id: range2079130
version: 1
direction: direction/forward
top level: BBa_K404210
start: 43
end: 45
orientation: inline

downstream 453
type: SequenceAnnotation
persistent identity: annotation2079128
display id: annotation2079128
version: 1
top level: BBa_K404210
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404210/annotation2079128/range2079128/1

https://synbiohub.org/public/igem/BBa_K404210/annotation2079128/range2079128/1
type: Range
persistent identity: range2079128
display id: range2079128
version: 1
direction: direction/forward
top level: BBa_K404210
start: 64
end: 72
orientation: inline

upstream 587
type: SequenceAnnotation
persistent identity: annotation2079126
display id: annotation2079126
version: 1
top level: BBa_K404210
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404210/annotation2079126/range2079126/1

https://synbiohub.org/public/igem/BBa_K404210/annotation2079126/range2079126/1
type: Range
persistent identity: range2079126
display id: range2079126
version: 1
direction: direction/forward
top level: BBa_K404210
start: 10
end: 42
orientation: inline

R=>A
type: SequenceAnnotation
persistent identity: annotation2079129
display id: annotation2079129
version: 1
top level: BBa_K404210
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K404210/annotation2079129/range2079129/1

https://synbiohub.org/public/igem/BBa_K404210/annotation2079129/range2079129/1
type: Range
persistent identity: range2079129
display id: range2079129
version: 1
direction: direction/forward
top level: BBa_K404210
start: 34
end: 36
orientation: inline

BBa_K404150
type: ComponentDefinition
persistent identity: BBa K404150
display id: BBa_K404150
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: [AAV2]-VP23
creator: Freiburg Bioware 2010
created: 2010-10-10T11:00:00Z
modified: 2015-05-08T01:12:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 6733
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: VP3
sequence annotation: VP2
sequence: https://synbiohub.org/public/igem/BBa_K404150_sequence/1

VP3
type: SequenceAnnotation
persistent identity: annotation2093338
display id: annotation2093338
version: 1
top level: BBa_K404150
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404150/annotation2093338/range2093338/1

https://synbiohub.org/public/igem/BBa_K404150/annotation2093338/range2093338/1
type: Range
persistent identity: range2093338
display id: range2093338
version: 1
direction: direction/forward
top level: BBa_K404150
start: 193
end: 1794
orientation: inline

VP2
type: SequenceAnnotation
persistent identity: annotation2093337
display id: annotation2093337
version: 1
top level: BBa_K404150
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404150/annotation2093337/range2093337/1

https://synbiohub.org/public/igem/BBa_K404150/annotation2093337/range2093337/1
type: Range
persistent identity: range2093337
display id: range2093337
version: 1
direction: direction/forward
top level: BBa_K404150
start: 1
end: 1794
orientation: inline

BBa_K404252
type: ComponentDefinition
persistent identity: BBa K404252
display id: BBa_K404252
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pCMV_[AAV2]-VP123ex (ViralBrick-587KO-Empty)
creator: Freiburg Bioware 2010
created: 2010-10-13T11:00:00Z
modified: 2015-05-08T01:12:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 7049
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: CMV promoter
sequence annotation: downstream 598
sequence annotation: HSPG-KO
sequence annotation: upstream 587
sequence annotation: AAV-2-cap (Stratagene)
sequence annotation: VP1
sequence annotation: VP2
sequence annotation: VP3
sequence: https://synbiohub.org/public/igem/BBa_K404252_sequence/1

CMV promoter
type: SequenceAnnotation
persistent identity: annotation2094562
display id: annotation2094562
version: 1
top level: BBa_K404252
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K404252/annotation2094562/range2094562/1

https://synbiohub.org/public/igem/BBa_K404252/annotation2094562/range2094562/1
type: Range
persistent identity: range2094562
display id: range2094562
version: 1
direction: direction/forward
top level: BBa_K404252
start: 2
end: 655
orientation: inline

downstream 598
type: SequenceAnnotation
persistent identity: annotation2094568
display id: annotation2094568
version: 1
top level: BBa_K404252
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404252/annotation2094568/range2094568/1

https://synbiohub.org/public/igem/BBa_K404252/annotation2094568/range2094568/1
type: Range
persistent identity: range2094568
display id: range2094568
version: 1
direction: direction/forward
top level: BBa_K404252
start: 2429
end: 2444
orientation: inline

HSPG-KO
type: SequenceAnnotation
persistent identity: annotation2101877
display id: annotation2101877
version: 1
top level: BBa_K404252
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K404252/annotation2101877/range2101877/1

https://synbiohub.org/public/igem/BBa_K404252/annotation2101877/range2101877/1
type: Range
persistent identity: range2101877
display id: range2101877
version: 1
direction: direction/forward
top level: BBa_K404252
start: 2429
end: 2431
orientation: inline

upstream 587
type: SequenceAnnotation
persistent identity: annotation2094567
display id: annotation2094567
version: 1
top level: BBa_K404252
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404252/annotation2094567/range2094567/1

https://synbiohub.org/public/igem/BBa_K404252/annotation2094567/range2094567/1
type: Range
persistent identity: range2094567
display id: range2094567
version: 1
direction: direction/forward
top level: BBa_K404252
start: 2397
end: 2428
orientation: inline

HSPG-KO
type: SequenceAnnotation
persistent identity: annotation2101878
display id: annotation2101878
version: 1
top level: BBa_K404252
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K404252/annotation2101878/range2101878/1

https://synbiohub.org/public/igem/BBa_K404252/annotation2101878/range2101878/1
type: Range
persistent identity: range2101878
display id: range2101878
version: 1
direction: direction/forward
top level: BBa_K404252
start: 2419
end: 2421
orientation: inline

AAV-2-cap (Stratagene)
type: SequenceAnnotation
persistent identity: annotation2094564
display id: annotation2094564
version: 1
top level: BBa_K404252
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404252/annotation2094564/range2094564/1

https://synbiohub.org/public/igem/BBa_K404252/annotation2094564/range2094564/1
type: Range
persistent identity: range2094564
display id: range2094564
version: 1
direction: direction/forward
top level: BBa_K404252
start: 671
end: 2998
orientation: inline

VP1
type: SequenceAnnotation
persistent identity: annotation2094563
display id: annotation2094563
version: 1
top level: BBa_K404252
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404252/annotation2094563/range2094563/1

https://synbiohub.org/public/igem/BBa_K404252/annotation2094563/range2094563/1
type: Range
persistent identity: range2094563
display id: range2094563
version: 1
direction: direction/forward
top level: BBa_K404252
start: 671
end: 2875
orientation: inline

VP2
type: SequenceAnnotation
persistent identity: annotation2094565
display id: annotation2094565
version: 1
top level: BBa_K404252
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404252/annotation2094565/range2094565/1

https://synbiohub.org/public/igem/BBa_K404252/annotation2094565/range2094565/1
type: Range
persistent identity: range2094565
display id: range2094565
version: 1
direction: direction/forward
top level: BBa_K404252
start: 1079
end: 2875
orientation: inline

VP3
type: SequenceAnnotation
persistent identity: annotation2094566
display id: annotation2094566
version: 1
top level: BBa_K404252
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404252/annotation2094566/range2094566/1

https://synbiohub.org/public/igem/BBa_K404252/annotation2094566/range2094566/1
type: Range
persistent identity: range2094566
display id: range2094566
version: 1
direction: direction/forward
top level: BBa_K404252
start: 1274
end: 2875
orientation: inline

hygR
type: ComponentDefinition
persistent identity: BBa K678021
display id: BBa_K678021
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Hygromycin cassette
creator: DTU-Denmark-2
created: 2011-09-03T11:00:00Z
modified: 2015-05-08T01:13:04Z
discontinued: false
dominant: false
experience: experience/None
group u list: _882_
m user id: 0
owner id: 8341
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Hygromycin cassette for selection of mammalian cells. The selection cassette contains the SV40 promoter, the hygromycin resistance gene, and the SV40 poly A as terminator
mutable notes: Compatible with the Plu'n'Play assembly standard
mutable provenance: From plasmid pcDNA3.1/Hygro from Invitrogen
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Other
role: SO:0000110
sequence annotation: Hygromycin cassette
sequence annotation: Linker 5
sequence: https://synbiohub.org/public/igem/BBa_K678021_sequence/1

Hygromycin cassette
type: SequenceAnnotation
persistent identity: annotation2127357
display id: annotation2127357
version: 1
top level: hygR
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K678021/annotation2127357/range2127357/1

https://synbiohub.org/public/igem/BBa_K678021/annotation2127357/range2127357/1
type: Range
persistent identity: range2127357
display id: range2127357
version: 1
direction: direction/forward
top level: hygR
start: 9
end: 1759
orientation: inline

Linker 5
type: SequenceAnnotation
persistent identity: annotation2127356
display id: annotation2127356
version: 1
top level: hygR
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K678021/annotation2127356/range2127356/1

https://synbiohub.org/public/igem/BBa_K678021/annotation2127356/range2127356/1
type: Range
persistent identity: range2127356
display id: range2127356
version: 1
direction: direction/forward
top level: hygR
start: 1
end: 8
orientation: inline

BBa_K404124
type: ComponentDefinition
persistent identity: BBa K404124
display id: BBa_K404124
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: [AAV2]-left-ITR_phTERT_betaglobin_mGMK_TK30_hGH_[AAV2]-right-ITR
creator: Freiburg Bioware 2010
created: 2010-10-10T11:00:00Z
modified: 2015-05-08T01:12:23Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 6733
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: y
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: left ITR
sequence annotation: hGH
sequence annotation: phTERT
sequence annotation: mGMK_TK30
sequence annotation: right ITR
sequence annotation: Beta-Globin-intron
sequence: https://synbiohub.org/public/igem/BBa_K404124_sequence/1

left ITR
type: SequenceAnnotation
persistent identity: annotation2095364
display id: annotation2095364
version: 1
top level: BBa_K404124
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404124/annotation2095364/range2095364/1

https://synbiohub.org/public/igem/BBa_K404124/annotation2095364/range2095364/1
type: Range
persistent identity: range2095364
display id: range2095364
version: 1
direction: direction/forward
top level: BBa_K404124
start: 1
end: 147
orientation: inline

hGH
type: SequenceAnnotation
persistent identity: annotation2095520
display id: annotation2095520
version: 1
top level: BBa_K404124
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404124/annotation2095520/range2095520/1

https://synbiohub.org/public/igem/BBa_K404124/annotation2095520/range2095520/1
type: Range
persistent identity: range2095520
display id: range2095520
version: 1
direction: direction/reverse
top level: BBa_K404124
start: 2831
end: 3309
orientation: inline

phTERT
type: SequenceAnnotation
persistent identity: annotation2095415
display id: annotation2095415
version: 1
top level: BBa_K404124
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404124/annotation2095415/range2095415/1

https://synbiohub.org/public/igem/BBa_K404124/annotation2095415/range2095415/1
type: Range
persistent identity: range2095415
display id: range2095415
version: 1
direction: direction/forward
top level: BBa_K404124
start: 154
end: 612
orientation: inline

mGMK_TK30
type: SequenceAnnotation
persistent identity: annotation2095518
display id: annotation2095518
version: 1
top level: BBa_K404124
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404124/annotation2095518/range2095518/1

https://synbiohub.org/public/igem/BBa_K404124/annotation2095518/range2095518/1
type: Range
persistent identity: range2095518
display id: range2095518
version: 1
direction: direction/forward
top level: BBa_K404124
start: 1119
end: 2822
orientation: inline

right ITR
type: SequenceAnnotation
persistent identity: annotation2095578
display id: annotation2095578
version: 1
top level: BBa_K404124
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404124/annotation2095578/range2095578/1

https://synbiohub.org/public/igem/BBa_K404124/annotation2095578/range2095578/1
type: Range
persistent identity: range2095578
display id: range2095578
version: 1
direction: direction/forward
top level: BBa_K404124
start: 3324
end: 3461
orientation: inline

Beta-Globin-intron
type: SequenceAnnotation
persistent identity: annotation2095416
display id: annotation2095416
version: 1
top level: BBa_K404124
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404124/annotation2095416/range2095416/1

https://synbiohub.org/public/igem/BBa_K404124/annotation2095416/range2095416/1
type: Range
persistent identity: range2095416
display id: range2095416
version: 1
direction: direction/forward
top level: BBa_K404124
start: 620
end: 1112
orientation: inline

BBa_K404224
type: ComponentDefinition
persistent identity: BBa K404224
display id: BBa_K404224
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: [AAV2]-Rep-VP123_P5-TATAless (ViralBrick-453-Z34C)
creator: Freiburg Bioware 2010
created: 2010-08-27T11:00:00Z
modified: 2015-05-08T01:12:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 7049
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: Rep 78
sequence annotation: Z34C
sequence annotation: VP1
sequence annotation: VP3
sequence annotation: Rep 52
sequence annotation: VP2
sequence annotation: Rep 40
sequence annotation: Rep 68
sequence annotation: AAV-2 cap (Stratagene)
sequence annotation: p5 TATAless
sequence: https://synbiohub.org/public/igem/BBa_K404224_sequence/1

Rep 78
type: SequenceAnnotation
persistent identity: annotation2098422
display id: annotation2098422
version: 1
top level: BBa_K404224
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404224/annotation2098422/range2098422/1

https://synbiohub.org/public/igem/BBa_K404224/annotation2098422/range2098422/1
type: Range
persistent identity: range2098422
display id: range2098422
version: 1
direction: direction/forward
top level: BBa_K404224
start: 2
end: 1867
orientation: inline

Z34C
type: SequenceAnnotation
persistent identity: annotation2098940
display id: annotation2098940
version: 1
top level: BBa_K404224
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404224/annotation2098940/range2098940/1

https://synbiohub.org/public/igem/BBa_K404224/annotation2098940/range2098940/1
type: Range
persistent identity: range2098940
display id: range2098940
version: 1
direction: direction/forward
top level: BBa_K404224
start: 3242
end: 3343
orientation: inline

VP1
type: SequenceAnnotation
persistent identity: annotation2098551
display id: annotation2098551
version: 1
top level: BBa_K404224
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404224/annotation2098551/range2098551/1

https://synbiohub.org/public/igem/BBa_K404224/annotation2098551/range2098551/1
type: Range
persistent identity: range2098551
display id: range2098551
version: 1
direction: direction/forward
top level: BBa_K404224
start: 1883
end: 4192
orientation: inline

VP3
type: SequenceAnnotation
persistent identity: annotation2098524
display id: annotation2098524
version: 1
top level: BBa_K404224
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404224/annotation2098524/range2098524/1

https://synbiohub.org/public/igem/BBa_K404224/annotation2098524/range2098524/1
type: Range
persistent identity: range2098524
display id: range2098524
version: 1
direction: direction/forward
top level: BBa_K404224
start: 2489
end: 4192
orientation: inline

Rep 52
type: SequenceAnnotation
persistent identity: annotation2098427
display id: annotation2098427
version: 1
top level: BBa_K404224
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404224/annotation2098427/range2098427/1

https://synbiohub.org/public/igem/BBa_K404224/annotation2098427/range2098427/1
type: Range
persistent identity: range2098427
display id: range2098427
version: 1
direction: direction/forward
top level: BBa_K404224
start: 673
end: 1867
orientation: inline

VP2
type: SequenceAnnotation
persistent identity: annotation2098458
display id: annotation2098458
version: 1
top level: BBa_K404224
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404224/annotation2098458/range2098458/1

https://synbiohub.org/public/igem/BBa_K404224/annotation2098458/range2098458/1
type: Range
persistent identity: range2098458
display id: range2098458
version: 1
direction: direction/forward
top level: BBa_K404224
start: 2294
end: 4192
orientation: inline

Rep 40
type: SequenceAnnotation
persistent identity: annotation2098428
display id: annotation2098428
version: 1
top level: BBa_K404224
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404224/annotation2098428/range2098428/1

https://synbiohub.org/public/igem/BBa_K404224/annotation2098428/range2098428/1
type: Range
persistent identity: range2098428
display id: range2098428
version: 1
direction: direction/forward
top level: BBa_K404224
start: 673
end: 1933
orientation: inline

Rep 68
type: SequenceAnnotation
persistent identity: annotation2098424
display id: annotation2098424
version: 1
top level: BBa_K404224
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404224/annotation2098424/range2098424/1

https://synbiohub.org/public/igem/BBa_K404224/annotation2098424/range2098424/1
type: Range
persistent identity: range2098424
display id: range2098424
version: 1
direction: direction/forward
top level: BBa_K404224
start: 2
end: 1933
orientation: inline

AAV-2 cap (Stratagene)
type: SequenceAnnotation
persistent identity: annotation2098661
display id: annotation2098661
version: 1
top level: BBa_K404224
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404224/annotation2098661/range2098661/1

https://synbiohub.org/public/igem/BBa_K404224/annotation2098661/range2098661/1
type: Range
persistent identity: range2098661
display id: range2098661
version: 1
direction: direction/forward
top level: BBa_K404224
start: 1883
end: 3795
orientation: inline

p5 TATAless
type: SequenceAnnotation
persistent identity: annotation2098845
display id: annotation2098845
version: 1
top level: BBa_K404224
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404224/annotation2098845/range2098845/1

https://synbiohub.org/public/igem/BBa_K404224/annotation2098845/range2098845/1
type: Range
persistent identity: range2098845
display id: range2098845
version: 1
direction: direction/forward
top level: BBa_K404224
start: 4324
end: 4487
orientation: inline

BBa_K1720003
type: ComponentDefinition
persistent identity: BBa K1720003
display id: BBa_K1720003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Human phosphodiesterase 5A gene silencing device NO.1
creator: Ying Guan, Yuanbin Cui
created: 2015-08-26T11:00:00Z
modified: 2015-09-18T07:29:39Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2140_
m user id: 25248
owner id: 25248
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This device is uesd for silencing the human phosphodiesterase 5A (PDE5A) gene.A U6 promoter driving a designed, synthetic shRNA-like miRNA followed by the terminator. PDE5A is a cGMP-binding, cGMP-specific phosphodiesterase, a member of the cyclic nucleotide phosphodiesterase family. This phosphodiesterase specifically hydrolyzes cGMP to 5'-GMP. It is involved in the regulation of intracellular concentrations of cyclic nucleotides and is important for smooth muscle relaxation in the cardiovascular system. We designed 3 silencing device and test their function at the same time.Here is the another two device :BBa_K1720004,BBaK172005 This device with a GFP reporter was then transfected into HEK293 cells by lentiviral vector.Once we silence the PDE5A gene the level of cGMP will be up regulated as a result. The positive control was HEK293 cells that treat with Sodium Nitroprusside ,a NO donator that activate sGC and up regulate the level of cGMP. A negative control was made by transfecting an empty vector that does not contain sGC subunit. We used Elisa to detect cGMP level. The results are as follow:
mutable notes: 1. Kukreja RC, Salloum FN, Das A: Cyclic Guanosine Monophosphate Signaling and Phosphodiesterase-5 Inhibitors in Cardioprotection. Journal Of the American College Of Cardiology 2012, 59(22):1921-1927. 2. Li L, Haider HK, Wang L, Lu G, Ashraf M: Adenoviral short hairpin RNA therapy targeting phosphodiesterase 5a relieves cardiac remodeling and dysfunction following myocardial infarction. American Journal Of Physiology-Heart And Circulatory Physiology 2012, 302(10):H2112-H2121. 3. Unwalla HJ, Li HT, Bahner I, Li MJ, Kohn D, Rossi JJ: Novel Pol II fusion promoter directs human immunodeficiency virus type 1-inducible coexpression of a short hairpin RNA and protein. Journal of virology 2006, 80(4):1863-1873.
mutable provenance: We designed the shRNA according to the homology segment sequence of PDE5A from NCBI
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
sequence: https://synbiohub.org/public/igem/BBa_K1720003_sequence/1

BBa_K1926022
type: ComponentDefinition
persistent identity: BBa K1926022
display id: BBa_K1926022
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: The SNAP-mCHERRY UNIT
creator: Xinyi Liu
created: 2016-10-09T11:00:00Z
modified: 2016-10-11T06:31:46Z
discontinued: false
dominant: false
group u list: _2393_
m user id: 28998
owner id: 28998
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This part is the SNAP-mCHERRY UNIT of cyclebow including a SNAP UNIT and a mCHERRY UNIT. The SNAP UNIT, which can be cut off by CRE, includes an sv40 nuclear location sequence (NLS), a covalent labeling of fusion proteins named SNAP-tag?? and an sv40 terminator flanked by homodromous recognition site of recombinase CRE named loxP. The mCHERRY UNIT, which can be cut off by VIKA, includes a degradation tag named DBOX, a fluorescent gene mCherry and an sv40 terminator flanked by homodromous recognition site of recombinase VIKA named Vox. You may use this part to: 1) Test the cut-off efficiency of recombinase CRE and VIKA; 2) Estimate the time needed for genome repair because mCherry can only be expressed after the cut off of SNAP UNIT and genome repair; 3) Fast cut off this part by transient transfecting recombinase gene into nucleus; 4) When there is no CRE, you can use this part as the SNAP UNIT, which can help localize proteins in vivo with BLOCK and dye from NEB company.
mutable notes: None
mutable provenance: The SNAP-tag?? was brought from NEB company. NLS and sv40 terminator were got from commercial plasmid: pentry nls GFP/pcdna3.1. The sequence of Vox and DBOX was retrieved from Addgene and got through denovo synthesis. mCHERRY was got from commercial plasmid: pentry dcas9 mcherry bira.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1926011
component: BBa_K1926013
sequence annotation: BBa_K1926011
sequence annotation: BBa_K1926013
sequence: https://synbiohub.org/public/igem/BBa_K1926022_sequence/1

BBa_K1926011
type: Component
persistent identity: component2491934
display id: component2491934
version: 1
top level: BBa_K1926022
access: public
definition: BBa_K1926011

BBa_K1926013
type: Component
persistent identity: component2491941
display id: component2491941
version: 1
top level: BBa_K1926022
access: public
definition: BBa_K1926013

BBa_K1926011
type: SequenceAnnotation
persistent identity: annotation2491934
display id: annotation2491934
version: 1
top level: BBa_K1926022
location: https://synbiohub.org/public/igem/BBa_K1926022/annotation2491934/range2491934/1
component: BBa_K1926011

https://synbiohub.org/public/igem/BBa_K1926022/annotation2491934/range2491934/1
type: Range
persistent identity: range2491934
display id: range2491934
version: 1
direction: direction/reverse
top level: BBa_K1926022
start: 1
end: 788
orientation: inline

BBa_K1926013
type: SequenceAnnotation
persistent identity: annotation2491941
display id: annotation2491941
version: 1
top level: BBa_K1926022
location: https://synbiohub.org/public/igem/BBa_K1926022/annotation2491941/range2491941/1
component: BBa_K1926013

https://synbiohub.org/public/igem/BBa_K1926022/annotation2491941/range2491941/1
type: Range
persistent identity: range2491941
display id: range2491941
version: 1
direction: direction/reverse
top level: BBa_K1926022
start: 789
end: 1704
orientation: inline

VP64
type: ComponentDefinition
persistent identity: BBa J176013
display id: BBa_J176013
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: VP64
creator: Karmella Haynes
created: 2011-09-28T11:00:00Z
modified: 2015-08-31T04:08:33Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _863_
m user id: 0
owner id: 10865
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Tetrameric VP16 transc. activator domain
mutable notes: TBA
mutable provenance: TBA
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: VP16
sequence: https://synbiohub.org/public/igem/BBa_J176013_sequence/1

VP16
type: SequenceAnnotation
persistent identity: annotation2166132
display id: annotation2166132
version: 1
top level: VP64
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_J176013/annotation2166132/range2166132/1

https://synbiohub.org/public/igem/BBa_J176013/annotation2166132/range2166132/1
type: Range
persistent identity: range2166132
display id: range2166132
version: 1
direction: direction/forward
top level: VP64
start: 118
end: 150
orientation: inline

VP16
type: SequenceAnnotation
persistent identity: annotation2166129
display id: annotation2166129
version: 1
top level: VP64
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_J176013/annotation2166129/range2166129/1

https://synbiohub.org/public/igem/BBa_J176013/annotation2166129/range2166129/1
type: Range
persistent identity: range2166129
display id: range2166129
version: 1
direction: direction/forward
top level: VP64
start: 1
end: 33
orientation: inline

VP16
type: SequenceAnnotation
persistent identity: annotation2166130
display id: annotation2166130
version: 1
top level: VP64
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_J176013/annotation2166130/range2166130/1

https://synbiohub.org/public/igem/BBa_J176013/annotation2166130/range2166130/1
type: Range
persistent identity: range2166130
display id: range2166130
version: 1
direction: direction/forward
top level: VP64
start: 40
end: 72
orientation: inline

VP16
type: SequenceAnnotation
persistent identity: annotation2166131
display id: annotation2166131
version: 1
top level: VP64
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_J176013/annotation2166131/range2166131/1

https://synbiohub.org/public/igem/BBa_J176013/annotation2166131/range2166131/1
type: Range
persistent identity: range2166131
display id: range2166131
version: 1
direction: direction/forward
top level: VP64
start: 79
end: 111
orientation: inline

IscA1
type: ComponentDefinition
persistent identity: BBa M36907
display id: BBa_M36907
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Coding Sequence of IscA1 pigeon homologue (codon-optimized for humans)
creator: Michael Becich, Thomas Lau, Sreyas Misra
created: 2015-10-24T11:00:00Z
modified: 2015-12-04T07:45:15Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _848_
m user id: 29123
owner id: 29123
owning group id: 9
status: status/Planning
bookmark: true
mutable description: The gene Iron-Sulfur cluster assembly 1 (IscA1) is a mitochondrial protein involved in the biogenesis and assembly of iron-sulfur clusters, which play a role in electron-transfer reactions and is theorized to be associated with certain organisms' magnetic perception. The homing pigeon is one such organism with this ability, and here we have codon-optimized the Columba Livia homologue for homo sapiens. The sequence can be inserted into plasmids for expression of the magnetoreceptor IscA gene, which hopefully will create magnetogenetic biosensors in the form of iron-sulfur cluster, for downstream output and applications.
mutable notes: While the plasmid design for this insert can be complicated, the coding sequence itself is quite simple. We only needed to remove the start codon in order to co-express a fluorescent protein, and modify two BbsII restriction enzyme sites by altering the third base pair of codons (wobble effect) at bp locations 75 (C->G) and 309 (A->G).
mutable provenance: The predicted IscA amino acid sequence was obtained from NCBI (http://www.ncbi.nlm.nih.gov/protein/XP_005508102.1), and codon-optimized for humans with IDT's tools.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_M36907_sequence/1

BBa_I712021
type: ComponentDefinition
persistent identity: BBa I712021
display id: BBa_I712021
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Interferon beta
creator: Anja Korenčič
created: 2007-10-21T11:00:00Z
modified: 2015-08-31T04:07:46Z
discontinued: false
dominant: false
group u list: _130_
m user id: 0
owner id: 1846
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Homo sapiens interferon beta.
mutable notes: PstI restriction site was mutated. Includes start and stop codon.
mutable provenance: Part comes from Origene human cDNA clone (NM_002176.2).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_I712021_sequence/1

BBa_I712002
type: ComponentDefinition
persistent identity: BBa I712002
display id: BBa_I712002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CCR5
creator: Andrej Ondracka
created: 2007-10-20T11:00:00Z
modified: 2015-08-31T04:07:45Z
discontinued: false
dominant: false
group u list: _130_
m user id: 0
owner id: 1896
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Chemokine (C-C motif) receptor 5, a HIV-1 co-receptor.
mutable notes: The part is cloned into BBa_J52017, a vector with eukaryontic terminator. PstI and EcoRI restriction sites inside CCR5 gene were mutated.
mutable provenance: CCR5 gene was obtained from Origene.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: CCR5
sequence: https://synbiohub.org/public/igem/BBa_I712002_sequence/1

CCR5
type: SequenceAnnotation
persistent identity: annotation1951768
display id: annotation1951768
version: 1
top level: BBa_I712002
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_I712002/annotation1951768/range1951768/1

https://synbiohub.org/public/igem/BBa_I712002/annotation1951768/range1951768/1
type: Range
persistent identity: range1951768
display id: range1951768
version: 1
direction: direction/forward
top level: BBa_I712002
start: 1
end: 1059
orientation: inline

BBa_K404165
type: ComponentDefinition
persistent identity: BBa K404165
display id: BBa_K404165
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pCMV_VP1up_NLS_mVenus_[AAV2]-VP23 (ViralBrick-587KO-Empty)
creator: Freiburg Bioware 2010
created: 2010-10-10T11:00:00Z
modified: 2015-05-08T01:12:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 6733
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: AAP
sequence annotation: VP 2
sequence annotation: HSPG-KO
sequence annotation: NLS
sequence annotation: CMV
sequence annotation: VP 1
sequence annotation: VP 1 up
sequence annotation: mVenus
sequence annotation: VP 3
sequence annotation: AAV-2 cap (Stratagene)
sequence: https://synbiohub.org/public/igem/BBa_K404165_sequence/1

AAP
type: SequenceAnnotation
persistent identity: annotation2099101
display id: annotation2099101
version: 1
top level: BBa_K404165
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404165/annotation2099101/range2099101/1

https://synbiohub.org/public/igem/BBa_K404165/annotation2099101/range2099101/1
type: Range
persistent identity: range2099101
display id: range2099101
version: 1
direction: direction/forward
top level: BBa_K404165
start: 1947
end: 2561
orientation: inline

VP 2
type: SequenceAnnotation
persistent identity: annotation2099098
display id: annotation2099098
version: 1
top level: BBa_K404165
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404165/annotation2099098/range2099098/1

https://synbiohub.org/public/igem/BBa_K404165/annotation2099098/range2099098/1
type: Range
persistent identity: range2099098
display id: range2099098
version: 1
direction: direction/forward
top level: BBa_K404165
start: 1835
end: 3628
orientation: inline

HSPG-KO
type: SequenceAnnotation
persistent identity: annotation2101816
display id: annotation2101816
version: 1
top level: BBa_K404165
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K404165/annotation2101816/range2101816/1

https://synbiohub.org/public/igem/BBa_K404165/annotation2101816/range2101816/1
type: Range
persistent identity: range2101816
display id: range2101816
version: 1
direction: direction/forward
top level: BBa_K404165
start: 3182
end: 3184
orientation: inline

NLS
type: SequenceAnnotation
persistent identity: annotation2099096
display id: annotation2099096
version: 1
top level: BBa_K404165
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404165/annotation2099096/range2099096/1

https://synbiohub.org/public/igem/BBa_K404165/annotation2099096/range2099096/1
type: Range
persistent identity: range2099096
display id: range2099096
version: 1
direction: direction/forward
top level: BBa_K404165
start: 1088
end: 1108
orientation: inline

CMV
type: SequenceAnnotation
persistent identity: annotation2099094
display id: annotation2099094
version: 1
top level: BBa_K404165
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404165/annotation2099094/range2099094/1

https://synbiohub.org/public/igem/BBa_K404165/annotation2099094/range2099094/1
type: Range
persistent identity: range2099094
display id: range2099094
version: 1
direction: direction/forward
top level: BBa_K404165
start: 2
end: 655
orientation: inline

VP 1
type: SequenceAnnotation
persistent identity: annotation2099100
display id: annotation2099100
version: 1
top level: BBa_K404165
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404165/annotation2099100/range2099100/1

https://synbiohub.org/public/igem/BBa_K404165/annotation2099100/range2099100/1
type: Range
persistent identity: range2099100
display id: range2099100
version: 1
direction: direction/forward
top level: BBa_K404165
start: 1835
end: 3628
orientation: inline

VP 1 up
type: SequenceAnnotation
persistent identity: annotation2099095
display id: annotation2099095
version: 1
top level: BBa_K404165
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404165/annotation2099095/range2099095/1

https://synbiohub.org/public/igem/BBa_K404165/annotation2099095/range2099095/1
type: Range
persistent identity: range2099095
display id: range2099095
version: 1
direction: direction/forward
top level: BBa_K404165
start: 673
end: 1081
orientation: inline

mVenus
type: SequenceAnnotation
persistent identity: annotation2099097
display id: annotation2099097
version: 1
top level: BBa_K404165
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404165/annotation2099097/range2099097/1

https://synbiohub.org/public/igem/BBa_K404165/annotation2099097/range2099097/1
type: Range
persistent identity: range2099097
display id: range2099097
version: 1
direction: direction/forward
top level: BBa_K404165
start: 1115
end: 1828
orientation: inline

VP 3
type: SequenceAnnotation
persistent identity: annotation2099093
display id: annotation2099093
version: 1
top level: BBa_K404165
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404165/annotation2099093/range2099093/1

https://synbiohub.org/public/igem/BBa_K404165/annotation2099093/range2099093/1
type: Range
persistent identity: range2099093
display id: range2099093
version: 1
direction: direction/forward
top level: BBa_K404165
start: 2027
end: 3628
orientation: inline

AAV-2 cap (Stratagene)
type: SequenceAnnotation
persistent identity: annotation2099099
display id: annotation2099099
version: 1
top level: BBa_K404165
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404165/annotation2099099/range2099099/1

https://synbiohub.org/public/igem/BBa_K404165/annotation2099099/range2099099/1
type: Range
persistent identity: range2099099
display id: range2099099
version: 1
direction: direction/forward
top level: BBa_K404165
start: 1835
end: 3751
orientation: inline

HSPG-KO
type: SequenceAnnotation
persistent identity: annotation2101817
display id: annotation2101817
version: 1
top level: BBa_K404165
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K404165/annotation2101817/range2101817/1

https://synbiohub.org/public/igem/BBa_K404165/annotation2101817/range2101817/1
type: Range
persistent identity: range2101817
display id: range2101817
version: 1
direction: direction/forward
top level: BBa_K404165
start: 3173
end: 3175
orientation: inline

BBa_K404229
type: ComponentDefinition
persistent identity: BBa K404229
display id: BBa_K404229
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: [AAV2]-Rep-VP123_P5-TATAless (ViralBrick-587KO-BAP)
creator: Freiburg Bioware 2010
created: 2010-08-27T11:00:00Z
modified: 2015-05-08T01:12:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 7049
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: Rep68
sequence annotation: HSPG-KO
sequence annotation: p5TATAless
sequence annotation: upstream 587
sequence annotation: BAP
sequence annotation: Rep40
sequence annotation: GLS-spacer
sequence annotation: downstream 587
sequence annotation: VP1
sequence annotation: Rep52
sequence annotation: VP2
sequence annotation: VP3
sequence annotation: Rep78
sequence annotation: TG-spacer
sequence: https://synbiohub.org/public/igem/BBa_K404229_sequence/1

Rep68
type: SequenceAnnotation
persistent identity: annotation2098608
display id: annotation2098608
version: 1
top level: BBa_K404229
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404229/annotation2098608/range2098608/1

https://synbiohub.org/public/igem/BBa_K404229/annotation2098608/range2098608/1
type: Range
persistent identity: range2098608
display id: range2098608
version: 1
direction: direction/forward
top level: BBa_K404229
start: 2
end: 1933
orientation: inline

HSPG-KO
type: SequenceAnnotation
persistent identity: annotation2101847
display id: annotation2101847
version: 1
top level: BBa_K404229
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K404229/annotation2101847/range2101847/1

https://synbiohub.org/public/igem/BBa_K404229/annotation2101847/range2101847/1
type: Range
persistent identity: range2101847
display id: range2101847
version: 1
direction: direction/forward
top level: BBa_K404229
start: 3635
end: 3637
orientation: inline

HSPG-KO
type: SequenceAnnotation
persistent identity: annotation2101846
display id: annotation2101846
version: 1
top level: BBa_K404229
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K404229/annotation2101846/range2101846/1

https://synbiohub.org/public/igem/BBa_K404229/annotation2101846/range2101846/1
type: Range
persistent identity: range2101846
display id: range2101846
version: 1
direction: direction/forward
top level: BBa_K404229
start: 3696
end: 3698
orientation: inline

p5TATAless
type: SequenceAnnotation
persistent identity: annotation2098912
display id: annotation2098912
version: 1
top level: BBa_K404229
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K404229/annotation2098912/range2098912/1

https://synbiohub.org/public/igem/BBa_K404229/annotation2098912/range2098912/1
type: Range
persistent identity: range2098912
display id: range2098912
version: 1
direction: direction/forward
top level: BBa_K404229
start: 4282
end: 4445
orientation: inline

upstream 587
type: SequenceAnnotation
persistent identity: annotation2098994
display id: annotation2098994
version: 1
top level: BBa_K404229
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404229/annotation2098994/range2098994/1

https://synbiohub.org/public/igem/BBa_K404229/annotation2098994/range2098994/1
type: Range
persistent identity: range2098994
display id: range2098994
version: 1
direction: direction/forward
top level: BBa_K404229
start: 3612
end: 3643
orientation: inline

BAP
type: SequenceAnnotation
persistent identity: annotation2098961
display id: annotation2098961
version: 1
top level: BBa_K404229
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404229/annotation2098961/range2098961/1

https://synbiohub.org/public/igem/BBa_K404229/annotation2098961/range2098961/1
type: Range
persistent identity: range2098961
display id: range2098961
version: 1
direction: direction/forward
top level: BBa_K404229
start: 3650
end: 3694
orientation: inline

Rep40
type: SequenceAnnotation
persistent identity: annotation2098709
display id: annotation2098709
version: 1
top level: BBa_K404229
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404229/annotation2098709/range2098709/1

https://synbiohub.org/public/igem/BBa_K404229/annotation2098709/range2098709/1
type: Range
persistent identity: range2098709
display id: range2098709
version: 1
direction: direction/forward
top level: BBa_K404229
start: 673
end: 1933
orientation: inline

GLS-spacer
type: SequenceAnnotation
persistent identity: annotation2098993
display id: annotation2098993
version: 1
top level: BBa_K404229
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404229/annotation2098993/range2098993/1

https://synbiohub.org/public/igem/BBa_K404229/annotation2098993/range2098993/1
type: Range
persistent identity: range2098993
display id: range2098993
version: 1
direction: direction/forward
top level: BBa_K404229
start: 3695
end: 3703
orientation: inline

downstream 587
type: SequenceAnnotation
persistent identity: annotation2098995
display id: annotation2098995
version: 1
top level: BBa_K404229
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404229/annotation2098995/range2098995/1

https://synbiohub.org/public/igem/BBa_K404229/annotation2098995/range2098995/1
type: Range
persistent identity: range2098995
display id: range2098995
version: 1
direction: direction/forward
top level: BBa_K404229
start: 3704
end: 3719
orientation: inline

VP1
type: SequenceAnnotation
persistent identity: annotation2098773
display id: annotation2098773
version: 1
top level: BBa_K404229
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404229/annotation2098773/range2098773/1

https://synbiohub.org/public/igem/BBa_K404229/annotation2098773/range2098773/1
type: Range
persistent identity: range2098773
display id: range2098773
version: 1
direction: direction/forward
top level: BBa_K404229
start: 1883
end: 4150
orientation: inline

Rep52
type: SequenceAnnotation
persistent identity: annotation2098660
display id: annotation2098660
version: 1
top level: BBa_K404229
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404229/annotation2098660/range2098660/1

https://synbiohub.org/public/igem/BBa_K404229/annotation2098660/range2098660/1
type: Range
persistent identity: range2098660
display id: range2098660
version: 1
direction: direction/forward
top level: BBa_K404229
start: 673
end: 1867
orientation: inline

VP2
type: SequenceAnnotation
persistent identity: annotation2098811
display id: annotation2098811
version: 1
top level: BBa_K404229
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404229/annotation2098811/range2098811/1

https://synbiohub.org/public/igem/BBa_K404229/annotation2098811/range2098811/1
type: Range
persistent identity: range2098811
display id: range2098811
version: 1
direction: direction/forward
top level: BBa_K404229
start: 2294
end: 4150
orientation: inline

VP3
type: SequenceAnnotation
persistent identity: annotation2098861
display id: annotation2098861
version: 1
top level: BBa_K404229
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404229/annotation2098861/range2098861/1

https://synbiohub.org/public/igem/BBa_K404229/annotation2098861/range2098861/1
type: Range
persistent identity: range2098861
display id: range2098861
version: 1
direction: direction/forward
top level: BBa_K404229
start: 2489
end: 4150
orientation: inline

Rep78
type: SequenceAnnotation
persistent identity: annotation2098552
display id: annotation2098552
version: 1
top level: BBa_K404229
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404229/annotation2098552/range2098552/1

https://synbiohub.org/public/igem/BBa_K404229/annotation2098552/range2098552/1
type: Range
persistent identity: range2098552
display id: range2098552
version: 1
direction: direction/forward
top level: BBa_K404229
start: 2
end: 1867
orientation: inline

TG-spacer
type: SequenceAnnotation
persistent identity: annotation2098988
display id: annotation2098988
version: 1
top level: BBa_K404229
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404229/annotation2098988/range2098988/1

https://synbiohub.org/public/igem/BBa_K404229/annotation2098988/range2098988/1
type: Range
persistent identity: range2098988
display id: range2098988
version: 1
direction: direction/forward
top level: BBa_K404229
start: 3644
end: 3649
orientation: inline

BBa_J52026
type: ComponentDefinition
persistent identity: BBa J52026
display id: BBa_J52026
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: dnMyD88-linker-GFP
creator: Jelka Pohar
created: 2006-10-14T11:00:00Z
modified: 2016-01-26T09:39:38Z
discontinued: false
dominant: false
group u list: _80_
m user id: 4206
owner id: 855
owning group id: 80
sample status: In stock
status: status/Available
bookmark: false
mutable description: dnMyD88 is a dominant negative form of protein MyD88 (an important protein in signaling cascade through TLR4 receptor). dnMyD88 is linked to GFP (acts as a reporter) through six aminoacid long protein linker.
mutable notes: dnMyD88-linker-GFP was made by using PCR overlap extension technique. This part is cloned in BioBrick vector pSB1AK3. Original sequence has PstI restriction site so we have to introduce a mutation without affect on amino acid sequence.
mutable provenance: dnMyD88- GFP- more to come
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: dnMyD88
sequence annotation: linker
sequence annotation: GFP
sequence annotation: dnMyD88-linker-GFP
sequence: https://synbiohub.org/public/igem/BBa_J52026_sequence/1

dnMyD88
type: SequenceAnnotation
persistent identity: annotation1903008
display id: annotation1903008
version: 1
top level: BBa_J52026
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J52026/annotation1903008/range1903008/1

https://synbiohub.org/public/igem/BBa_J52026/annotation1903008/range1903008/1
type: Range
persistent identity: range1903008
display id: range1903008
version: 1
direction: direction/forward
top level: BBa_J52026
start: 1
end: 417
orientation: inline

linker
type: SequenceAnnotation
persistent identity: annotation1903009
display id: annotation1903009
version: 1
top level: BBa_J52026
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_J52026/annotation1903009/range1903009/1

https://synbiohub.org/public/igem/BBa_J52026/annotation1903009/range1903009/1
type: Range
persistent identity: range1903009
display id: range1903009
version: 1
direction: direction/forward
top level: BBa_J52026
start: 418
end: 435
orientation: inline

GFP
type: SequenceAnnotation
persistent identity: annotation1903010
display id: annotation1903010
version: 1
top level: BBa_J52026
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J52026/annotation1903010/range1903010/1

https://synbiohub.org/public/igem/BBa_J52026/annotation1903010/range1903010/1
type: Range
persistent identity: range1903010
display id: range1903010
version: 1
direction: direction/forward
top level: BBa_J52026
start: 436
end: 1155
orientation: inline

dnMyD88-linker-GFP
type: SequenceAnnotation
persistent identity: annotation1903011
display id: annotation1903011
version: 1
top level: BBa_J52026
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_J52026/annotation1903011/range1903011/1

https://synbiohub.org/public/igem/BBa_J52026/annotation1903011/range1903011/1
type: Range
persistent identity: range1903011
display id: range1903011
version: 1
direction: direction/forward
top level: BBa_J52026
start: 1
end: 1155
orientation: inline

BBa_K404108
type: ComponentDefinition
persistent identity: BBa K404108
display id: BBa_K404108
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: hGH terminator
creator: Freiburg Bioware 2010
created: 2010-08-27T11:00:00Z
modified: 2015-05-08T01:12:23Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 7049
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: hGH terminator
sequence: https://synbiohub.org/public/igem/BBa_K404108_sequence/1

hGH terminator
type: SequenceAnnotation
persistent identity: annotation2093173
display id: annotation2093173
version: 1
top level: BBa_K404108
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404108/annotation2093173/range2093173/1

https://synbiohub.org/public/igem/BBa_K404108/annotation2093173/range2093173/1
type: Range
persistent identity: range2093173
display id: range2093173
version: 1
direction: direction/forward
top level: BBa_K404108
start: 1
end: 481
orientation: inline

BBa_K1777019
type: ComponentDefinition
persistent identity: BBa K1777019
display id: BBa_K1777019
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: flexible linker
creator: Xuhang Li
created: 2015-09-17T11:00:00Z
modified: 2015-09-20T02:06:57Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2199_
m user id: 27798
owner id: 25645
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This a flexible linker combined by two former linkers, which can be easily connect to the end of protein with overlap pcr.
mutable notes: when we chose a linker for our fusion protein，we would have wanted to use -gggsgggs- linker. But we found that such a tandem repeating linker is hard to work well with overlap PCR because the OE region should be unique. Therefore, we improved the original part by change the first GGGS to a carefully selected natural linker from linker database to avoid repeating sequence. Besides, we used the selected natural linker to adjust its length to about 50A.
mutable provenance: BBa_K1486003
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: natural linker
sequence annotation: GS linker
sequence: https://synbiohub.org/public/igem/BBa_K1777019_sequence/1

natural linker
type: SequenceAnnotation
persistent identity: annotation2476148
display id: annotation2476148
version: 1
top level: BBa_K1777019
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1777019/annotation2476148/range2476148/1

https://synbiohub.org/public/igem/BBa_K1777019/annotation2476148/range2476148/1
type: Range
persistent identity: range2476148
display id: range2476148
version: 1
direction: direction/forward
top level: BBa_K1777019
start: 1
end: 21
orientation: inline

GS linker
type: SequenceAnnotation
persistent identity: annotation2476149
display id: annotation2476149
version: 1
top level: BBa_K1777019
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1777019/annotation2476149/range2476149/1

https://synbiohub.org/public/igem/BBa_K1777019/annotation2476149/range2476149/1
type: Range
persistent identity: range2476149
display id: range2476149
version: 1
direction: direction/forward
top level: BBa_K1777019
start: 22
end: 36
orientation: inline

IscA2
type: ComponentDefinition
persistent identity: BBa M36908
display id: BBa_M36908
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Coding Sequence of IscA2 pigeon homologue (codon-optimized for humans)
creator: Michael Becich
created: 2015-10-24T11:00:00Z
modified: 2015-12-04T08:06:13Z
discontinued: false
dominant: false
experience: experience/Fails
group u list: _848_
m user id: 29123
owner id: 29123
owning group id: 9
status: status/Planning
bookmark: false
mutable description: The gene Iron-Sulfur cluster assembly 2 (IscA2) is a mitochondrial protein involved in the biogenesis and assembly of iron-sulfur clusters, which play a role in electron-transfer reactions and is theorized to be associated with certain organisms' magnetic perception. The homing pigeon is one such organism with this ability, and here we have codon-optimized the Columba Livia homologue for homo sapiens. The sequence can be inserted into plasmids for expression of the magnetoreceptor IscA gene, which hopefully will create magnetogenetic biosensors in the form of iron-sulfur cluster, for downstream output and applications.
mutable notes: While the plasmid design for this insert can be complicated, the coding sequence itself is quite simple. We only needed to add the start codon since this second isoform did not have methionine built in.
mutable provenance: The predicted IscA amino acid sequence was obtained from NCBI (http://www.ncbi.nlm.nih.gov/protein/XP_005502393.1), and codon-optimized for humans with IDT's tools.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316

BBa_K404201
type: ComponentDefinition
persistent identity: BBa K404201
display id: BBa_K404201
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: ViralBrick-453-BAP
creator: Freiburg Bioware 2010
created: 2010-08-27T11:00:00Z
modified: 2015-05-08T01:12:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 7049
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part
mutable notes: This part
mutable provenance: Synthetic sequence
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: A Spacer
sequence annotation: downstream 453
sequence annotation: BAP
sequence annotation: upstream 453
sequence: https://synbiohub.org/public/igem/BBa_K404201_sequence/1

A Spacer
type: SequenceAnnotation
persistent identity: annotation2091219
display id: annotation2091219
version: 1
top level: BBa_K404201
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404201/annotation2091219/range2091219/1

https://synbiohub.org/public/igem/BBa_K404201/annotation2091219/range2091219/1
type: Range
persistent identity: range2091219
display id: range2091219
version: 1
direction: direction/forward
top level: BBa_K404201
start: 43
end: 45
orientation: inline

downstream 453
type: SequenceAnnotation
persistent identity: annotation2091222
display id: annotation2091222
version: 1
top level: BBa_K404201
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404201/annotation2091222/range2091222/1

https://synbiohub.org/public/igem/BBa_K404201/annotation2091222/range2091222/1
type: Range
persistent identity: range2091222
display id: range2091222
version: 1
direction: direction/forward
top level: BBa_K404201
start: 94
end: 114
orientation: inline

BAP
type: SequenceAnnotation
persistent identity: annotation2091220
display id: annotation2091220
version: 1
top level: BBa_K404201
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404201/annotation2091220/range2091220/1

https://synbiohub.org/public/igem/BBa_K404201/annotation2091220/range2091220/1
type: Range
persistent identity: range2091220
display id: range2091220
version: 1
direction: direction/forward
top level: BBa_K404201
start: 46
end: 90
orientation: inline

A Spacer
type: SequenceAnnotation
persistent identity: annotation2091221
display id: annotation2091221
version: 1
top level: BBa_K404201
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404201/annotation2091221/range2091221/1

https://synbiohub.org/public/igem/BBa_K404201/annotation2091221/range2091221/1
type: Range
persistent identity: range2091221
display id: range2091221
version: 1
direction: direction/forward
top level: BBa_K404201
start: 91
end: 93
orientation: inline

upstream 453
type: SequenceAnnotation
persistent identity: annotation2091218
display id: annotation2091218
version: 1
top level: BBa_K404201
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404201/annotation2091218/range2091218/1

https://synbiohub.org/public/igem/BBa_K404201/annotation2091218/range2091218/1
type: Range
persistent identity: range2091218
display id: range2091218
version: 1
direction: direction/forward
top level: BBa_K404201
start: 1
end: 42
orientation: inline

BBa_K1926002
type: ComponentDefinition
persistent identity: BBa K1926002
display id: BBa_K1926002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: A cyclic promoter of Ki-67 from human genome
creator: Xinyi Liu
created: 2016-10-08T11:00:00Z
modified: 2016-10-18T10:57:50Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2393_
m user id: 28998
owner id: 28998
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This part is a cyclic promoter named Ki67 from human genome. It can lead to transcription of the downstream DNA sequence once in every G1 phase[3]. 3. Pei, D.S., et al., Analysis of human Ki-67 gene promoter and identification of the Sp1 binding sites for Ki-67 transcription. Tumour Biol, 2012. 33(1): p. 257-66. You may use this part to: 1) Express something in mammal cell lines particularly in G1 phases or once in every cell cycle by stable transfecting it into cell line; 2) Use it as a human promoter by transient transfecting it into cells.
mutable notes: Mutation to remove Pst1
mutable provenance: The sequence was retrieved from Addgene. We got it from human genome through PCR using the following primers: pKi67-F: ACCTCTGCCCTCCGCCAGCCG pKi67-R: ACCCGGTGGCCCTACAGGCTACG (Product: 360)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: P Ki67
sequence: https://synbiohub.org/public/igem/BBa_K1926002_sequence/1

P Ki67
type: SequenceAnnotation
persistent identity: annotation2491729
display id: annotation2491729
version: 1
top level: BBa_K1926002
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1926002/annotation2491729/range2491729/1

https://synbiohub.org/public/igem/BBa_K1926002/annotation2491729/range2491729/1
type: Range
persistent identity: range2491729
display id: range2491729
version: 1
direction: direction/forward
top level: BBa_K1926002
start: 65
end: 424
orientation: inline

BBa_K404235
type: ComponentDefinition
persistent identity: BBa K404235
display id: BBa_K404235
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: [AAV2]Rep-VP123_p5TATAless (ViralBrick 453 Z34C-587 HSPG-KO)
creator: Freiburg Bioware 2010
created: 2010-10-24T11:00:00Z
modified: 2015-05-08T01:12:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 6674
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: Rep40
sequence annotation: HSPG-KO
sequence annotation: VP1
sequence annotation: p5TATAless
sequence annotation: Rep68
sequence annotation: VP2
sequence annotation: Rep78
sequence annotation: AAV-2-cap (Stratagene)
sequence annotation: Z34C
sequence annotation: Rep52
sequence annotation: VP3
sequence: https://synbiohub.org/public/igem/BBa_K404235_sequence/1

Rep40
type: SequenceAnnotation
persistent identity: annotation2098417
display id: annotation2098417
version: 1
top level: BBa_K404235
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404235/annotation2098417/range2098417/1

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HSPG-KO
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VP1
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p5TATAless
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Rep68
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HSPG-KO
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VP2
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Rep78
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AAV-2-cap (Stratagene)
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Z34C
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Rep52
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VP3
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BBa_K404209
type: ComponentDefinition
persistent identity: BBa K404209
display id: BBa_K404209
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: ViralBrick-587KO-BAP
creator: Freiburg Bioware 2010
created: 2010-08-27T11:00:00Z
modified: 2015-05-08T01:12:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
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sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: upstream 587
sequence annotation: TG Spacer
sequence annotation: downstream 587
sequence annotation: R=>A
sequence annotation: BAP-motif
sequence annotation: downstream 453
sequence annotation: GLS Spacer
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sequence: https://synbiohub.org/public/igem/BBa_K404209_sequence/1

upstream 587
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TG Spacer
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downstream 587
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R=>A
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BAP-motif
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downstream 453
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GLS Spacer
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display id: annotation2079121
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R=>A
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upstream 453
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BBa_K404253
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display id: BBa_K404253
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pCMV_[AAV2]-VP123ex (ViralBrick-587KO-His-Tag)
creator: Freiburg Bioware 2010
created: 2010-10-13T11:00:00Z
modified: 2015-05-08T01:12:25Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
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sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
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sequence annotation: VP2
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sequence annotation: HSPG-KO
sequence annotation: His-tag
sequence annotation: CMV
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GLS-spacer
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VP2
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AAV-2-cap (Stratagene)
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HSPG-KO
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direction: direction/forward
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His-tag
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direction: direction/forward
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CMV
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TG-spacer
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VP1
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direction: direction/forward
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HSPG-KO
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VP3
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direction: direction/forward
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upstream 587
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direction: direction/forward
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orientation: inline

BBa_K404100
type: ComponentDefinition
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display id: BBa_K404100
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: [AAV2]-left-ITR
creator: Freiburg Bioware 2010
created: 2010-08-27T11:00:00Z
modified: 2015-05-08T01:12:23Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 7049
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
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misc
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version: 1
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role: SO:0000110
role: feature/misc
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display id: range2093166
version: 1
direction: direction/forward
top level: BBa_K404100
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end: 147
orientation: inline

BBa_K404246
type: ComponentDefinition
persistent identity: BBa K404246
display id: BBa_K404246
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pCMV_[AAV2]-VP123ex (ViralBrick-587-BAP)
creator: Freiburg Bioware 2010
created: 2010-10-13T11:00:00Z
modified: 2015-05-08T01:12:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 7049
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: TG-spacer
sequence annotation: AAV-2-cap (Stratagene)
sequence annotation: downstream 587
sequence annotation: VP3
sequence annotation: CMV promoter
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sequence annotation: BAP
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sequence annotation: VP1
sequence: https://synbiohub.org/public/igem/BBa_K404246_sequence/1

TG-spacer
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role: SO:0000110
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https://synbiohub.org/public/igem/BBa_K404246/annotation2094275/range2094275/1
type: Range
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display id: range2094275
version: 1
direction: direction/forward
top level: BBa_K404246
start: 2429
end: 2434
orientation: inline

AAV-2-cap (Stratagene)
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display id: annotation2094273
version: 1
top level: BBa_K404246
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404246/annotation2094273/range2094273/1

https://synbiohub.org/public/igem/BBa_K404246/annotation2094273/range2094273/1
type: Range
persistent identity: range2094273
display id: range2094273
version: 1
direction: direction/forward
top level: BBa_K404246
start: 671
end: 3058
orientation: inline

downstream 587
type: SequenceAnnotation
persistent identity: annotation2094277
display id: annotation2094277
version: 1
top level: BBa_K404246
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404246/annotation2094277/range2094277/1

https://synbiohub.org/public/igem/BBa_K404246/annotation2094277/range2094277/1
type: Range
persistent identity: range2094277
display id: range2094277
version: 1
direction: direction/forward
top level: BBa_K404246
start: 2489
end: 2504
orientation: inline

VP3
type: SequenceAnnotation
persistent identity: annotation2094271
display id: annotation2094271
version: 1
top level: BBa_K404246
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404246/annotation2094271/range2094271/1

https://synbiohub.org/public/igem/BBa_K404246/annotation2094271/range2094271/1
type: Range
persistent identity: range2094271
display id: range2094271
version: 1
direction: direction/forward
top level: BBa_K404246
start: 1274
end: 2935
orientation: inline

CMV promoter
type: SequenceAnnotation
persistent identity: annotation2094264
display id: annotation2094264
version: 1
top level: BBa_K404246
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K404246/annotation2094264/range2094264/1

https://synbiohub.org/public/igem/BBa_K404246/annotation2094264/range2094264/1
type: Range
persistent identity: range2094264
display id: range2094264
version: 1
direction: direction/forward
top level: BBa_K404246
start: 2
end: 655
orientation: inline

VP2
type: SequenceAnnotation
persistent identity: annotation2094267
display id: annotation2094267
version: 1
top level: BBa_K404246
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404246/annotation2094267/range2094267/1

https://synbiohub.org/public/igem/BBa_K404246/annotation2094267/range2094267/1
type: Range
persistent identity: range2094267
display id: range2094267
version: 1
direction: direction/forward
top level: BBa_K404246
start: 1079
end: 2935
orientation: inline

GLS-spacer
type: SequenceAnnotation
persistent identity: annotation2094276
display id: annotation2094276
version: 1
top level: BBa_K404246
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404246/annotation2094276/range2094276/1

https://synbiohub.org/public/igem/BBa_K404246/annotation2094276/range2094276/1
type: Range
persistent identity: range2094276
display id: range2094276
version: 1
direction: direction/forward
top level: BBa_K404246
start: 2480
end: 2488
orientation: inline

BAP
type: SequenceAnnotation
persistent identity: annotation2094274
display id: annotation2094274
version: 1
top level: BBa_K404246
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404246/annotation2094274/range2094274/1

https://synbiohub.org/public/igem/BBa_K404246/annotation2094274/range2094274/1
type: Range
persistent identity: range2094274
display id: range2094274
version: 1
direction: direction/forward
top level: BBa_K404246
start: 2435
end: 2479
orientation: inline

upstream 587
type: SequenceAnnotation
persistent identity: annotation2094278
display id: annotation2094278
version: 1
top level: BBa_K404246
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404246/annotation2094278/range2094278/1

https://synbiohub.org/public/igem/BBa_K404246/annotation2094278/range2094278/1
type: Range
persistent identity: range2094278
display id: range2094278
version: 1
direction: direction/forward
top level: BBa_K404246
start: 2397
end: 2428
orientation: inline

VP1
type: SequenceAnnotation
persistent identity: annotation2094266
display id: annotation2094266
version: 1
top level: BBa_K404246
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404246/annotation2094266/range2094266/1

https://synbiohub.org/public/igem/BBa_K404246/annotation2094266/range2094266/1
type: Range
persistent identity: range2094266
display id: range2094266
version: 1
direction: direction/forward
top level: BBa_K404246
start: 671
end: 2935
orientation: inline

BBa_K1119006
type: ComponentDefinition
persistent identity: BBa K1119006
display id: BBa_K1119006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CMV promoter
creator: Wai In Hui
created: 2013-09-15T11:00:00Z
modified: 2015-05-08T01:09:13Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1430_
m user id: 0
owner id: 16863
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: Constitutive promoter longer
mutable notes: no
mutable provenance: put in later
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: CMV promoter
sequence: https://synbiohub.org/public/igem/BBa_K1119006_sequence/1

CMV promoter
type: SequenceAnnotation
persistent identity: annotation2363279
display id: annotation2363279
version: 1
top level: BBa_K1119006
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1119006/annotation2363279/range2363279/1

https://synbiohub.org/public/igem/BBa_K1119006/annotation2363279/range2363279/1
type: Range
persistent identity: range2363279
display id: range2363279
version: 1
direction: direction/forward
top level: BBa_K1119006
start: 1
end: 618
orientation: inline

MLS
type: ComponentDefinition
persistent identity: BBa K1119000
display id: BBa_K1119000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Mitochondrial Leader Sequence in RFC10 standard
creator: Wai In Hui
created: 2013-09-10T11:00:00Z
modified: 2015-05-08T01:09:13Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1430_
m user id: 0
owner id: 16863
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: Mitochondrial leader sequence (MLS) helps direct protein to the mitochondria. When this sequence is cloned in frame at the N-terminus of the protein, it can target the recombinant protein to the mitochondria in mammalian cells. MLS will be removed upon translocation into the mitochondrial matrix. However, at least four additional amino acids (Ile-His-Ser-Leu) on this sequence are known to be retained on the N-terminus of the protein.
mutable notes: No
mutable provenance: Plasmid from Invitrogen: pCMV/myc/mito (http://tools.lifetechnologies.com/content/sfs/manuals/pshooter_pcmv_man.pdf)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: Mitochondrial Leader Sequence
sequence: https://synbiohub.org/public/igem/BBa_K1119000_sequence/1

Mitochondrial Leader Sequence
type: SequenceAnnotation
persistent identity: annotation2363256
display id: annotation2363256
version: 1
top level: MLS
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1119000/annotation2363256/range2363256/1

https://synbiohub.org/public/igem/BBa_K1119000/annotation2363256/range2363256/1
type: Range
persistent identity: range2363256
display id: range2363256
version: 1
direction: direction/forward
top level: MLS
start: 1
end: 87
orientation: inline

BBa_J52019
type: ComponentDefinition
persistent identity: BBa J52019
display id: BBa_J52019
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: dnTraf6
creator: Matej Skocaj
created: 2006-10-13T11:00:00Z
modified: 2015-08-31T03:54:04Z
discontinued: false
dominant: false
group u list: _80_
m user id: 0
owner id: 857
owning group id: 80
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: It is an imortant protein in signaling cascade through TLr receptors (MyD88 dependent pathway).
mutable notes: None.
mutable provenance: pDeNy
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: dnTraf6
sequence: https://synbiohub.org/public/igem/BBa_J52019_sequence/1

dnTraf6
type: SequenceAnnotation
persistent identity: annotation1903258
display id: annotation1903258
version: 1
top level: BBa_J52019
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_J52019/annotation1903258/range1903258/1

https://synbiohub.org/public/igem/BBa_J52019/annotation1903258/range1903258/1
type: Range
persistent identity: range1903258
display id: range1903258
version: 1
direction: direction/forward
top level: BBa_J52019
start: 1
end: 714
orientation: inline

dnTraf6
type: SequenceAnnotation
persistent identity: annotation1903257
display id: annotation1903257
version: 1
top level: BBa_J52019
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J52019/annotation1903257/range1903257/1

https://synbiohub.org/public/igem/BBa_J52019/annotation1903257/range1903257/1
type: Range
persistent identity: range1903257
display id: range1903257
version: 1
direction: direction/forward
top level: BBa_J52019
start: 1
end: 714
orientation: inline

BBa_K404010
type: ComponentDefinition
persistent identity: BBa K404010
display id: BBa_K404010
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: [AAV2]-VP2
creator: Freiburg Bioware 2010
created: 2010-10-20T11:00:00Z
modified: 2015-05-08T01:12:23Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 6674
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: VP2
sequence: https://synbiohub.org/public/igem/BBa_K404010_sequence/1

VP2
type: SequenceAnnotation
persistent identity: annotation2093158
display id: annotation2093158
version: 1
top level: BBa_K404010
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404010/annotation2093158/range2093158/1

https://synbiohub.org/public/igem/BBa_K404010/annotation2093158/range2093158/1
type: Range
persistent identity: range2093158
display id: range2093158
version: 1
direction: direction/forward
top level: BBa_K404010
start: 1
end: 1830
orientation: inline

BBa_K1720005
type: ComponentDefinition
persistent identity: BBa K1720005
display id: BBa_K1720005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Human phosphodiesterase 5A gene silencing device NO.3
creator: Ying Guan , Yuanbin Cui
created: 2015-08-26T11:00:00Z
modified: 2015-09-18T07:28:43Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2140_
m user id: 25248
owner id: 25248
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This device is uesd for silencing the human phosphodiesterase 5A (PDE5A) gene.A U6 promoter driving a designed, synthetic shRNA-like miRNA followed by the terminator. PDE5A is a cGMP-binding, cGMP-specific phosphodiesterase, a member of the cyclic nucleotide phosphodiesterase family. This phosphodiesterase specifically hydrolyzes cGMP to 5'-GMP. It is involved in the regulation of intracellular concentrations of cyclic nucleotides and is important for smooth muscle relaxation in the cardiovascular system. We designed 3 silencing device and test their function at the same time.Here is the another two device :BBa_K1720004,BBaK172005 This device with a GFP reporter was then transfected into HEK293 cells by lentiviral vector.Once we silence the PDE5A gene the level of cGMP will be up regulated as a result. The positive control was HEK293 cells that treat with Sodium Nitroprusside ,a NO donator that activate sGC and up regulate the level of cGMP. A negative control was made by transfecting an empty vector that does not contain sGC subunit. We used Elisa to detect cGMP level. The results are as follow:
mutable notes: 1. Kukreja RC, Salloum FN, Das A: Cyclic Guanosine Monophosphate Signaling and Phosphodiesterase-5 Inhibitors in Cardioprotection. Journal Of the American College Of Cardiology 2012, 59(22):1921-1927. 2. Li L, Haider HK, Wang L, Lu G, Ashraf M: Adenoviral short hairpin RNA therapy targeting phosphodiesterase 5a relieves cardiac remodeling and dysfunction following myocardial infarction. American Journal Of Physiology-Heart And Circulatory Physiology 2012, 302(10):H2112-H2121. 3. Unwalla HJ, Li HT, Bahner I, Li MJ, Kohn D, Rossi JJ: Novel Pol II fusion promoter directs human immunodeficiency virus type 1-inducible coexpression of a short hairpin RNA and protein. Journal of virology 2006, 80(4):1863-1873.
mutable provenance: We designed the shRNA according to the homology segment sequence of PDE5A from NCBI
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
sequence: https://synbiohub.org/public/igem/BBa_K1720005_sequence/1

BBa_J52013
type: ComponentDefinition
persistent identity: BBa J52013
display id: BBa_J52013
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: dnMyD88-linker-Rluc-linker-PEST191
creator: Monika Ciglic
created: 2006-10-13T11:00:00Z
modified: 2015-08-31T03:54:04Z
discontinued: false
dominant: false
group u list: _80_
m user id: 0
owner id: 800
owning group id: 80
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: dnMyD88-linker-Rluc-linker-PEST191 is a part where dnMyD88 (very important protein in signaling cascade through TLR4 receptor) is linked to Rluc (reporter) and PEST191 (degradation tag). Both linkers are made of six aminoacids.
mutable notes: dnMyD88-linker-Rluc-linker-PEST191 was made by using PCR ligation technique. It was difficult since we had some problems with primers. This part is cloned in BioBrick vector pSB1AK3.
mutable provenance: -
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: dnMyD88-linker-Rluc-linker-PEST191
sequence annotation: PEST191
sequence annotation: Rluc
sequence annotation: linker
sequence annotation: dnMyD88
sequence: https://synbiohub.org/public/igem/BBa_J52013_sequence/1

dnMyD88-linker-Rluc-linker-PEST191
type: SequenceAnnotation
persistent identity: annotation1902848
display id: annotation1902848
version: 1
top level: BBa_J52013
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_J52013/annotation1902848/range1902848/1

https://synbiohub.org/public/igem/BBa_J52013/annotation1902848/range1902848/1
type: Range
persistent identity: range1902848
display id: range1902848
version: 1
direction: direction/forward
top level: BBa_J52013
start: 1
end: 1872
orientation: inline

PEST191
type: SequenceAnnotation
persistent identity: annotation1902844
display id: annotation1902844
version: 1
top level: BBa_J52013
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_J52013/annotation1902844/range1902844/1

https://synbiohub.org/public/igem/BBa_J52013/annotation1902844/range1902844/1
type: Range
persistent identity: range1902844
display id: range1902844
version: 1
direction: direction/forward
top level: BBa_J52013
start: 1387
end: 1872
orientation: inline

Rluc
type: SequenceAnnotation
persistent identity: annotation1902842
display id: annotation1902842
version: 1
top level: BBa_J52013
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J52013/annotation1902842/range1902842/1

https://synbiohub.org/public/igem/BBa_J52013/annotation1902842/range1902842/1
type: Range
persistent identity: range1902842
display id: range1902842
version: 1
direction: direction/forward
top level: BBa_J52013
start: 436
end: 1368
orientation: inline

linker
type: SequenceAnnotation
persistent identity: annotation1902843
display id: annotation1902843
version: 1
top level: BBa_J52013
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_J52013/annotation1902843/range1902843/1

https://synbiohub.org/public/igem/BBa_J52013/annotation1902843/range1902843/1
type: Range
persistent identity: range1902843
display id: range1902843
version: 1
direction: direction/forward
top level: BBa_J52013
start: 1369
end: 1386
orientation: inline

dnMyD88
type: SequenceAnnotation
persistent identity: annotation1902840
display id: annotation1902840
version: 1
top level: BBa_J52013
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J52013/annotation1902840/range1902840/1

https://synbiohub.org/public/igem/BBa_J52013/annotation1902840/range1902840/1
type: Range
persistent identity: range1902840
display id: range1902840
version: 1
direction: direction/forward
top level: BBa_J52013
start: 1
end: 417
orientation: inline

linker
type: SequenceAnnotation
persistent identity: annotation1902841
display id: annotation1902841
version: 1
top level: BBa_J52013
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_J52013/annotation1902841/range1902841/1

https://synbiohub.org/public/igem/BBa_J52013/annotation1902841/range1902841/1
type: Range
persistent identity: range1902841
display id: range1902841
version: 1
direction: direction/forward
top level: BBa_J52013
start: 418
end: 435
orientation: inline

BBa_K258004
type: ComponentDefinition
persistent identity: BBa K258004
display id: BBa_K258004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Keratinocyte growth factor (KGF)
creator: Ozkan IS
created: 2009-10-03T11:00:00Z
modified: 2015-05-08T01:11:42Z
discontinued: false
dominant: false
experience: experience/None
group u list: _358_
m user id: 0
owner id: 4260
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: Keratinocyte growth factor (KGF) is a locally acting epithelial mitogen that is produced by cells of mesenchymal origin and has an important role in protecting and repairing epithelial tissues.Use of recombinant human KGF (palifermin) in patients with hematologic malignancies reduces the incidence and duration of severe oral mucositis experienced after intensive chemoradiotherapy. These results suggest that KGF may be useful in the treatment of patients with other kinds of tumors, including those of epithelial origin.[1]The potential effect of KGF on wound healing was assessed in vitro by measuring randomized migration and plasminogen activator (PA) activity of keratinocytes in response to the growth factor. Incubation of normal human keratinocytes with KGF in modified MCDB 153 medium significantly stimulated cell migration[2] The Keratinocyte Growth Factor (KGF), also known as FGF7, is a growth factor present in the epithelialization-phase of wound healing. In this phase, keratinocytes are covering the wound, forming the epithelium.[3] . KGF is weakly expressed in human skin, but is strongly upregulated in dermal fibroblasts after skin injury. Its binding to a transmembrane receptor on keratinocytes induces proliferation and migration of these cells. Furthermore, KGF has been shown to protect epithelial cells from the toxic effects of reactive oxygen species. We have identified a series of KGF-regulated genes that are likely to play a role in these processes. In addition to KGF, activin seems to be a novel player in wound healing.[4]
mutable notes: The gene is in pMA vector having biobrick restriction sites(Ecor1, Xba1 and Spe1,Pst1,respectively)
mutable provenance: Gene was synthesized at GENEART.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K258004_sequence/1

BBa_K404104
type: ComponentDefinition
persistent identity: BBa K404104
display id: BBa_K404104
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: p5-TATAless promoter
creator: Freiburg Bioware 2010
created: 2010-08-27T11:00:00Z
modified: 2015-05-08T01:12:23Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 7049
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: p5TATAless
sequence: https://synbiohub.org/public/igem/BBa_K404104_sequence/1

p5TATAless
type: SequenceAnnotation
persistent identity: annotation2094942
display id: annotation2094942
version: 1
top level: BBa_K404104
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K404104/annotation2094942/range2094942/1

https://synbiohub.org/public/igem/BBa_K404104/annotation2094942/range2094942/1
type: Range
persistent identity: range2094942
display id: range2094942
version: 1
direction: direction/forward
top level: BBa_K404104
start: 1
end: 164
orientation: inline

BBa_K404162
type: ComponentDefinition
persistent identity: BBa K404162
display id: BBa_K404162
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pCMV_Z-EGFR-1907_Middle-Linker_[AAV2]-VP23 (ViralBrick-587KO-BAP)
creator: Freiburg Bioware 2010
created: 2010-10-11T11:00:00Z
modified: 2015-05-08T01:12:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 6733
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: downstream 587
sequence annotation: BAP
sequence annotation: ZEGFR_1907
sequence annotation: HSPG-KO
sequence annotation: VP3
sequence annotation: CMV
sequence annotation: TG-spacer
sequence annotation: upstream 587
sequence annotation: GLS-spacer
sequence annotation: middle-linker
sequence annotation: VP2
sequence annotation: AAV-2-cap (Stratagene)
sequence: https://synbiohub.org/public/igem/BBa_K404162_sequence/1

downstream 587
type: SequenceAnnotation
persistent identity: annotation2095021
display id: annotation2095021
version: 1
top level: BBa_K404162
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404162/annotation2095021/range2095021/1

https://synbiohub.org/public/igem/BBa_K404162/annotation2095021/range2095021/1
type: Range
persistent identity: range2095021
display id: range2095021
version: 1
direction: direction/forward
top level: BBa_K404162
start: 2288
end: 2303
orientation: inline

BAP
type: SequenceAnnotation
persistent identity: annotation2095018
display id: annotation2095018
version: 1
top level: BBa_K404162
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404162/annotation2095018/range2095018/1

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type: Range
persistent identity: range2095018
display id: range2095018
version: 1
direction: direction/forward
top level: BBa_K404162
start: 2234
end: 2278
orientation: inline

ZEGFR_1907
type: SequenceAnnotation
persistent identity: annotation2095013
display id: annotation2095013
version: 1
top level: BBa_K404162
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404162/annotation2095013/range2095013/1

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type: Range
persistent identity: range2095013
display id: range2095013
version: 1
direction: direction/forward
top level: BBa_K404162
start: 671
end: 844
orientation: inline

HSPG-KO
type: SequenceAnnotation
persistent identity: annotation2101812
display id: annotation2101812
version: 1
top level: BBa_K404162
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K404162/annotation2101812/range2101812/1

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type: Range
persistent identity: range2101812
display id: range2101812
version: 1
direction: direction/forward
top level: BBa_K404162
start: 2219
end: 2221
orientation: inline

HSPG-KO
type: SequenceAnnotation
persistent identity: annotation2101813
display id: annotation2101813
version: 1
top level: BBa_K404162
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K404162/annotation2101813/range2101813/1

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type: Range
persistent identity: range2101813
display id: range2101813
version: 1
direction: direction/forward
top level: BBa_K404162
start: 2288
end: 2290
orientation: inline

VP3
type: SequenceAnnotation
persistent identity: annotation2095016
display id: annotation2095016
version: 1
top level: BBa_K404162
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404162/annotation2095016/range2095016/1

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type: Range
persistent identity: range2095016
display id: range2095016
version: 1
direction: direction/forward
top level: BBa_K404162
start: 1073
end: 2734
orientation: inline

CMV
type: SequenceAnnotation
persistent identity: annotation2095012
display id: annotation2095012
version: 1
top level: BBa_K404162
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K404162/annotation2095012/range2095012/1

https://synbiohub.org/public/igem/BBa_K404162/annotation2095012/range2095012/1
type: Range
persistent identity: range2095012
display id: range2095012
version: 1
direction: direction/forward
top level: BBa_K404162
start: 2
end: 655
orientation: inline

TG-spacer
type: SequenceAnnotation
persistent identity: annotation2095019
display id: annotation2095019
version: 1
top level: BBa_K404162
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404162/annotation2095019/range2095019/1

https://synbiohub.org/public/igem/BBa_K404162/annotation2095019/range2095019/1
type: Range
persistent identity: range2095019
display id: range2095019
version: 1
direction: direction/forward
top level: BBa_K404162
start: 2228
end: 2233
orientation: inline

upstream 587
type: SequenceAnnotation
persistent identity: annotation2095022
display id: annotation2095022
version: 1
top level: BBa_K404162
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404162/annotation2095022/range2095022/1

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type: Range
persistent identity: range2095022
display id: range2095022
version: 1
direction: direction/forward
top level: BBa_K404162
start: 2196
end: 2227
orientation: inline

GLS-spacer
type: SequenceAnnotation
persistent identity: annotation2095020
display id: annotation2095020
version: 1
top level: BBa_K404162
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404162/annotation2095020/range2095020/1

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type: Range
persistent identity: range2095020
display id: range2095020
version: 1
direction: direction/forward
top level: BBa_K404162
start: 2279
end: 2287
orientation: inline

middle-linker
type: SequenceAnnotation
persistent identity: annotation2095014
display id: annotation2095014
version: 1
top level: BBa_K404162
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404162/annotation2095014/range2095014/1

https://synbiohub.org/public/igem/BBa_K404162/annotation2095014/range2095014/1
type: Range
persistent identity: range2095014
display id: range2095014
version: 1
direction: direction/forward
top level: BBa_K404162
start: 851
end: 874
orientation: inline

VP2
type: SequenceAnnotation
persistent identity: annotation2095015
display id: annotation2095015
version: 1
top level: BBa_K404162
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404162/annotation2095015/range2095015/1

https://synbiohub.org/public/igem/BBa_K404162/annotation2095015/range2095015/1
type: Range
persistent identity: range2095015
display id: range2095015
version: 1
direction: direction/forward
top level: BBa_K404162
start: 881
end: 2734
orientation: inline

AAV-2-cap (Stratagene)
type: SequenceAnnotation
persistent identity: annotation2095017
display id: annotation2095017
version: 1
top level: BBa_K404162
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404162/annotation2095017/range2095017/1

https://synbiohub.org/public/igem/BBa_K404162/annotation2095017/range2095017/1
type: Range
persistent identity: range2095017
display id: range2095017
version: 1
direction: direction/forward
top level: BBa_K404162
start: 881
end: 2857
orientation: inline

BBa_K678012
type: ComponentDefinition
persistent identity: BBa K678012
display id: BBa_K678012
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: SV40 poly A, terminator for mammalian cells
creator: DTU-Denmark-2
created: 2011-09-03T11:00:00Z
modified: 2015-05-08T01:13:04Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _882_
m user id: 0
owner id: 8341
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: SV40 poly A, terminator for use in mammalian cells
mutable notes: Compatible with the Plug'n'Play assembly standard
mutable provenance: From plasmid pcDNA3.1 from Invitrogen
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: Linker 4
sequence annotation: SV40
sequence: https://synbiohub.org/public/igem/BBa_K678012_sequence/1

Linker 4
type: SequenceAnnotation
persistent identity: annotation2127354
display id: annotation2127354
version: 1
top level: BBa_K678012
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K678012/annotation2127354/range2127354/1

https://synbiohub.org/public/igem/BBa_K678012/annotation2127354/range2127354/1
type: Range
persistent identity: range2127354
display id: range2127354
version: 1
direction: direction/forward
top level: BBa_K678012
start: 1
end: 8
orientation: inline

SV40
type: SequenceAnnotation
persistent identity: annotation2127355
display id: annotation2127355
version: 1
top level: BBa_K678012
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_K678012/annotation2127355/range2127355/1

https://synbiohub.org/public/igem/BBa_K678012/annotation2127355/range2127355/1
type: Range
persistent identity: range2127355
display id: range2127355
version: 1
direction: direction/forward
top level: BBa_K678012
start: 9
end: 139
orientation: inline

BBa_K404212
type: ComponentDefinition
persistent identity: BBa K404212
display id: BBa_K404212
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: ViralBrick-587KO-RGD
creator: Freiburg Bioware 2010
created: 2010-08-27T11:00:00Z
modified: 2015-05-08T01:12:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 7049
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: downstream 453
sequence annotation: R=>A
sequence annotation: TG Spacer
sequence annotation: upstream 453
sequence annotation: upstream 587KO
sequence annotation: RGD-motif
sequence annotation: GLS Spacer
sequence annotation: downstream 587KO
sequence: https://synbiohub.org/public/igem/BBa_K404212_sequence/1

downstream 453
type: SequenceAnnotation
persistent identity: annotation2079148
display id: annotation2079148
version: 1
top level: BBa_K404212
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404212/annotation2079148/range2079148/1

https://synbiohub.org/public/igem/BBa_K404212/annotation2079148/range2079148/1
type: Range
persistent identity: range2079148
display id: range2079148
version: 1
direction: direction/forward
top level: BBa_K404212
start: 106
end: 114
orientation: inline

R=>A
type: SequenceAnnotation
persistent identity: annotation2079142
display id: annotation2079142
version: 1
top level: BBa_K404212
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K404212/annotation2079142/range2079142/1

https://synbiohub.org/public/igem/BBa_K404212/annotation2079142/range2079142/1
type: Range
persistent identity: range2079142
display id: range2079142
version: 1
direction: direction/forward
top level: BBa_K404212
start: 34
end: 36
orientation: inline

TG Spacer
type: SequenceAnnotation
persistent identity: annotation2079143
display id: annotation2079143
version: 1
top level: BBa_K404212
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404212/annotation2079143/range2079143/1

https://synbiohub.org/public/igem/BBa_K404212/annotation2079143/range2079143/1
type: Range
persistent identity: range2079143
display id: range2079143
version: 1
direction: direction/forward
top level: BBa_K404212
start: 43
end: 48
orientation: inline

R=>A
type: SequenceAnnotation
persistent identity: annotation2079144
display id: annotation2079144
version: 1
top level: BBa_K404212
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K404212/annotation2079144/range2079144/1

https://synbiohub.org/public/igem/BBa_K404212/annotation2079144/range2079144/1
type: Range
persistent identity: range2079144
display id: range2079144
version: 1
direction: direction/forward
top level: BBa_K404212
start: 85
end: 87
orientation: inline

upstream 453
type: SequenceAnnotation
persistent identity: annotation2079140
display id: annotation2079140
version: 1
top level: BBa_K404212
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404212/annotation2079140/range2079140/1

https://synbiohub.org/public/igem/BBa_K404212/annotation2079140/range2079140/1
type: Range
persistent identity: range2079140
display id: range2079140
version: 1
direction: direction/forward
top level: BBa_K404212
start: 1
end: 9
orientation: inline

upstream 587KO
type: SequenceAnnotation
persistent identity: annotation2079141
display id: annotation2079141
version: 1
top level: BBa_K404212
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404212/annotation2079141/range2079141/1

https://synbiohub.org/public/igem/BBa_K404212/annotation2079141/range2079141/1
type: Range
persistent identity: range2079141
display id: range2079141
version: 1
direction: direction/forward
top level: BBa_K404212
start: 10
end: 42
orientation: inline

RGD-motif
type: SequenceAnnotation
persistent identity: annotation2079145
display id: annotation2079145
version: 1
top level: BBa_K404212
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404212/annotation2079145/range2079145/1

https://synbiohub.org/public/igem/BBa_K404212/annotation2079145/range2079145/1
type: Range
persistent identity: range2079145
display id: range2079145
version: 1
direction: direction/forward
top level: BBa_K404212
start: 49
end: 75
orientation: inline

GLS Spacer
type: SequenceAnnotation
persistent identity: annotation2079146
display id: annotation2079146
version: 1
top level: BBa_K404212
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404212/annotation2079146/range2079146/1

https://synbiohub.org/public/igem/BBa_K404212/annotation2079146/range2079146/1
type: Range
persistent identity: range2079146
display id: range2079146
version: 1
direction: direction/forward
top level: BBa_K404212
start: 76
end: 87
orientation: inline

downstream 587KO
type: SequenceAnnotation
persistent identity: annotation2079147
display id: annotation2079147
version: 1
top level: BBa_K404212
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404212/annotation2079147/range2079147/1

https://synbiohub.org/public/igem/BBa_K404212/annotation2079147/range2079147/1
type: Range
persistent identity: range2079147
display id: range2079147
version: 1
direction: direction/forward
top level: BBa_K404212
start: 85
end: 105
orientation: inline

BBa_K404232
type: ComponentDefinition
persistent identity: BBa K404232
display id: BBa_K404232
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: [AAV2]-Rep-VP123_P5-TATAless (ViralBrick-587KO-RGD)
creator: Freiburg Bioware 2010
created: 2010-08-27T11:00:00Z
modified: 2015-05-08T01:12:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 7049
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: GLS-spacer
sequence annotation: Rep40
sequence annotation: TG-spacer
sequence annotation: Rep78
sequence annotation: upstream 587
sequence annotation: RGD
sequence annotation: Rep52
sequence annotation: HSPG-KO
sequence annotation: VP3
sequence annotation: downstream 587
sequence annotation: p5TATAless
sequence annotation: VP2
sequence annotation: Rep68
sequence annotation: VP1
sequence: https://synbiohub.org/public/igem/BBa_K404232_sequence/1

GLS-spacer
type: SequenceAnnotation
persistent identity: annotation2098548
display id: annotation2098548
version: 1
top level: BBa_K404232
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404232/annotation2098548/range2098548/1

https://synbiohub.org/public/igem/BBa_K404232/annotation2098548/range2098548/1
type: Range
persistent identity: range2098548
display id: range2098548
version: 1
direction: direction/forward
top level: BBa_K404232
start: 3677
end: 3685
orientation: inline

Rep40
type: SequenceAnnotation
persistent identity: annotation2098522
display id: annotation2098522
version: 1
top level: BBa_K404232
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404232/annotation2098522/range2098522/1

https://synbiohub.org/public/igem/BBa_K404232/annotation2098522/range2098522/1
type: Range
persistent identity: range2098522
display id: range2098522
version: 1
direction: direction/forward
top level: BBa_K404232
start: 673
end: 1933
orientation: inline

TG-spacer
type: SequenceAnnotation
persistent identity: annotation2098547
display id: annotation2098547
version: 1
top level: BBa_K404232
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404232/annotation2098547/range2098547/1

https://synbiohub.org/public/igem/BBa_K404232/annotation2098547/range2098547/1
type: Range
persistent identity: range2098547
display id: range2098547
version: 1
direction: direction/forward
top level: BBa_K404232
start: 3644
end: 3649
orientation: inline

Rep78
type: SequenceAnnotation
persistent identity: annotation2098519
display id: annotation2098519
version: 1
top level: BBa_K404232
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404232/annotation2098519/range2098519/1

https://synbiohub.org/public/igem/BBa_K404232/annotation2098519/range2098519/1
type: Range
persistent identity: range2098519
display id: range2098519
version: 1
direction: direction/forward
top level: BBa_K404232
start: 2
end: 1867
orientation: inline

upstream 587
type: SequenceAnnotation
persistent identity: annotation2098549
display id: annotation2098549
version: 1
top level: BBa_K404232
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404232/annotation2098549/range2098549/1

https://synbiohub.org/public/igem/BBa_K404232/annotation2098549/range2098549/1
type: Range
persistent identity: range2098549
display id: range2098549
version: 1
direction: direction/forward
top level: BBa_K404232
start: 3612
end: 3643
orientation: inline

RGD
type: SequenceAnnotation
persistent identity: annotation2098546
display id: annotation2098546
version: 1
top level: BBa_K404232
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404232/annotation2098546/range2098546/1

https://synbiohub.org/public/igem/BBa_K404232/annotation2098546/range2098546/1
type: Range
persistent identity: range2098546
display id: range2098546
version: 1
direction: direction/forward
top level: BBa_K404232
start: 3650
end: 3676
orientation: inline

Rep52
type: SequenceAnnotation
persistent identity: annotation2098521
display id: annotation2098521
version: 1
top level: BBa_K404232
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404232/annotation2098521/range2098521/1

https://synbiohub.org/public/igem/BBa_K404232/annotation2098521/range2098521/1
type: Range
persistent identity: range2098521
display id: range2098521
version: 1
direction: direction/forward
top level: BBa_K404232
start: 673
end: 1867
orientation: inline

HSPG-KO
type: SequenceAnnotation
persistent identity: annotation2101867
display id: annotation2101867
version: 1
top level: BBa_K404232
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K404232/annotation2101867/range2101867/1

https://synbiohub.org/public/igem/BBa_K404232/annotation2101867/range2101867/1
type: Range
persistent identity: range2101867
display id: range2101867
version: 1
direction: direction/forward
top level: BBa_K404232
start: 3678
end: 3680
orientation: inline

HSPG-KO
type: SequenceAnnotation
persistent identity: annotation2101868
display id: annotation2101868
version: 1
top level: BBa_K404232
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K404232/annotation2101868/range2101868/1

https://synbiohub.org/public/igem/BBa_K404232/annotation2101868/range2101868/1
type: Range
persistent identity: range2101868
display id: range2101868
version: 1
direction: direction/forward
top level: BBa_K404232
start: 3635
end: 3637
orientation: inline

VP3
type: SequenceAnnotation
persistent identity: annotation2098526
display id: annotation2098526
version: 1
top level: BBa_K404232
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404232/annotation2098526/range2098526/1

https://synbiohub.org/public/igem/BBa_K404232/annotation2098526/range2098526/1
type: Range
persistent identity: range2098526
display id: range2098526
version: 1
direction: direction/forward
top level: BBa_K404232
start: 2489
end: 4132
orientation: inline

downstream 587
type: SequenceAnnotation
persistent identity: annotation2098550
display id: annotation2098550
version: 1
top level: BBa_K404232
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404232/annotation2098550/range2098550/1

https://synbiohub.org/public/igem/BBa_K404232/annotation2098550/range2098550/1
type: Range
persistent identity: range2098550
display id: range2098550
version: 1
direction: direction/forward
top level: BBa_K404232
start: 3686
end: 3701
orientation: inline

p5TATAless
type: SequenceAnnotation
persistent identity: annotation2098545
display id: annotation2098545
version: 1
top level: BBa_K404232
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K404232/annotation2098545/range2098545/1

https://synbiohub.org/public/igem/BBa_K404232/annotation2098545/range2098545/1
type: Range
persistent identity: range2098545
display id: range2098545
version: 1
direction: direction/forward
top level: BBa_K404232
start: 4264
end: 4427
orientation: inline

VP2
type: SequenceAnnotation
persistent identity: annotation2098525
display id: annotation2098525
version: 1
top level: BBa_K404232
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404232/annotation2098525/range2098525/1

https://synbiohub.org/public/igem/BBa_K404232/annotation2098525/range2098525/1
type: Range
persistent identity: range2098525
display id: range2098525
version: 1
direction: direction/forward
top level: BBa_K404232
start: 2294
end: 4132
orientation: inline

Rep68
type: SequenceAnnotation
persistent identity: annotation2098520
display id: annotation2098520
version: 1
top level: BBa_K404232
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404232/annotation2098520/range2098520/1

https://synbiohub.org/public/igem/BBa_K404232/annotation2098520/range2098520/1
type: Range
persistent identity: range2098520
display id: range2098520
version: 1
direction: direction/forward
top level: BBa_K404232
start: 2
end: 1933
orientation: inline

VP1
type: SequenceAnnotation
persistent identity: annotation2098523
display id: annotation2098523
version: 1
top level: BBa_K404232
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404232/annotation2098523/range2098523/1

https://synbiohub.org/public/igem/BBa_K404232/annotation2098523/range2098523/1
type: Range
persistent identity: range2098523
display id: range2098523
version: 1
direction: direction/forward
top level: BBa_K404232
start: 1883
end: 4132
orientation: inline

BBa_I712012
type: ComponentDefinition
persistent identity: BBa I712012
display id: BBa_I712012
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TIR domain of TLR3
creator: Andrej Ondracka
created: 2007-10-20T11:00:00Z
modified: 2015-08-31T04:07:45Z
discontinued: false
dominant: false
group u list: _130_
m user id: 0
owner id: 1896
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: Toll-like receptor 3 cytoplasmic domain.
mutable notes: Cloned into pSBAK3 vector with terminator. Start codon is NOT included.
mutable provenance: amplified from TLR3 gene, obtained from
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: TIR domain
sequence: https://synbiohub.org/public/igem/BBa_I712012_sequence/1

TIR domain
type: SequenceAnnotation
persistent identity: annotation1951817
display id: annotation1951817
version: 1
top level: BBa_I712012
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_I712012/annotation1951817/range1951817/1

https://synbiohub.org/public/igem/BBa_I712012/annotation1951817/range1951817/1
type: Range
persistent identity: range1951817
display id: range1951817
version: 1
direction: direction/forward
top level: BBa_I712012
start: 1
end: 456
orientation: inline

BBa_K404202
type: ComponentDefinition
persistent identity: BBa K404202
display id: BBa_K404202
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: ViralBrick-453-His-Tag
creator: Freiburg Bioware 2010
created: 2010-08-27T11:00:00Z
modified: 2015-05-08T01:12:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 7049
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: upstream 453
sequence annotation: His-Tag
sequence annotation: A Spacer
sequence annotation: downstream 453
sequence: https://synbiohub.org/public/igem/BBa_K404202_sequence/1

upstream 453
type: SequenceAnnotation
persistent identity: annotation2078934
display id: annotation2078934
version: 1
top level: BBa_K404202
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404202/annotation2078934/range2078934/1

https://synbiohub.org/public/igem/BBa_K404202/annotation2078934/range2078934/1
type: Range
persistent identity: range2078934
display id: range2078934
version: 1
direction: direction/forward
top level: BBa_K404202
start: 1
end: 42
orientation: inline

His-Tag
type: SequenceAnnotation
persistent identity: annotation2078936
display id: annotation2078936
version: 1
top level: BBa_K404202
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404202/annotation2078936/range2078936/1

https://synbiohub.org/public/igem/BBa_K404202/annotation2078936/range2078936/1
type: Range
persistent identity: range2078936
display id: range2078936
version: 1
direction: direction/forward
top level: BBa_K404202
start: 46
end: 63
orientation: inline

A Spacer
type: SequenceAnnotation
persistent identity: annotation2078937
display id: annotation2078937
version: 1
top level: BBa_K404202
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404202/annotation2078937/range2078937/1

https://synbiohub.org/public/igem/BBa_K404202/annotation2078937/range2078937/1
type: Range
persistent identity: range2078937
display id: range2078937
version: 1
direction: direction/forward
top level: BBa_K404202
start: 64
end: 66
orientation: inline

downstream 453
type: SequenceAnnotation
persistent identity: annotation2078938
display id: annotation2078938
version: 1
top level: BBa_K404202
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404202/annotation2078938/range2078938/1

https://synbiohub.org/public/igem/BBa_K404202/annotation2078938/range2078938/1
type: Range
persistent identity: range2078938
display id: range2078938
version: 1
direction: direction/forward
top level: BBa_K404202
start: 67
end: 87
orientation: inline

A Spacer
type: SequenceAnnotation
persistent identity: annotation2078935
display id: annotation2078935
version: 1
top level: BBa_K404202
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404202/annotation2078935/range2078935/1

https://synbiohub.org/public/igem/BBa_K404202/annotation2078935/range2078935/1
type: Range
persistent identity: range2078935
display id: range2078935
version: 1
direction: direction/forward
top level: BBa_K404202
start: 43
end: 45
orientation: inline

BBa_K404101
type: ComponentDefinition
persistent identity: BBa K404101
display id: BBa_K404101
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: [AAV2]-right-ITR
creator: Freiburg Bioware 2010
created: 2010-08-27T11:00:00Z
modified: 2015-05-08T01:12:23Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 7049
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: right ITR
sequence: https://synbiohub.org/public/igem/BBa_K404101_sequence/1

right ITR
type: SequenceAnnotation
persistent identity: annotation2093167
display id: annotation2093167
version: 1
top level: BBa_K404101
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404101/annotation2093167/range2093167/1

https://synbiohub.org/public/igem/BBa_K404101/annotation2093167/range2093167/1
type: Range
persistent identity: range2093167
display id: range2093167
version: 1
direction: direction/forward
top level: BBa_K404101
start: 1
end: 138
orientation: inline

MLS
type: ComponentDefinition
persistent identity: BBa K1119001
display id: BBa_K1119001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Mitochondrial Leader Sequence with RFC25 standard
creator: Wai In Hui
created: 2013-09-10T11:00:00Z
modified: 2015-05-08T01:09:13Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1430_
m user id: 0
owner id: 16863
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: Mitochondrial leader sequence (MLS) helps direct protein to the mitochondria. When this sequence is cloned in frame at the N-terminus of the protein, it can target the recombinant protein to the mitochondria in mammalian cells. MLS will be removed upon translocation into the mitochondrial matrix. However, at least four additional amino acids (Ile-His-Ser-Leu) on this sequence are known to be retained on the N-terminus of the protein. Since MLS always fuses with protein for function, this BioBrick is in RFC 25 standard, which facilitate protein fusion in order to introduce other protein into mitochondria.
mutable notes: No
mutable provenance: Invitrogen: pCMV/myc/mito (http://tools.lifetechnologies.com/content/sfs/manuals/pshooter_pcmv_man.pdf)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: Mitochondrial Leader Sequence
sequence: https://synbiohub.org/public/igem/BBa_K1119001_sequence/1

Mitochondrial Leader Sequence
type: SequenceAnnotation
persistent identity: annotation2363257
display id: annotation2363257
version: 1
top level: MLS
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1119001/annotation2363257/range2363257/1

https://synbiohub.org/public/igem/BBa_K1119001/annotation2363257/range2363257/1
type: Range
persistent identity: range2363257
display id: range2363257
version: 1
direction: direction/forward
top level: MLS
start: 1
end: 87
orientation: inline

BBa_K404128
type: ComponentDefinition
persistent identity: BBa K404128
display id: BBa_K404128
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: [AAV2]-left-ITR_pCMV_mVenus_hGH_[AAV2]-right-ITR
creator: Freiburg Bioware 2010
created: 2010-10-10T11:00:00Z
modified: 2015-05-08T01:12:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 6733
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: hGH
sequence annotation: left ITR
sequence annotation: right ITR
sequence annotation: mVenus
sequence annotation: CMV promter
sequence: https://synbiohub.org/public/igem/BBa_K404128_sequence/1

hGH
type: SequenceAnnotation
persistent identity: annotation2093314
display id: annotation2093314
version: 1
top level: BBa_K404128
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404128/annotation2093314/range2093314/1

https://synbiohub.org/public/igem/BBa_K404128/annotation2093314/range2093314/1
type: Range
persistent identity: range2093314
display id: range2093314
version: 1
direction: direction/forward
top level: BBa_K404128
start: 1558
end: 2037
orientation: inline

left ITR
type: SequenceAnnotation
persistent identity: annotation2093305
display id: annotation2093305
version: 1
top level: BBa_K404128
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404128/annotation2093305/range2093305/1

https://synbiohub.org/public/igem/BBa_K404128/annotation2093305/range2093305/1
type: Range
persistent identity: range2093305
display id: range2093305
version: 1
direction: direction/forward
top level: BBa_K404128
start: 1
end: 147
orientation: inline

right ITR
type: SequenceAnnotation
persistent identity: annotation2093313
display id: annotation2093313
version: 1
top level: BBa_K404128
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404128/annotation2093313/range2093313/1

https://synbiohub.org/public/igem/BBa_K404128/annotation2093313/range2093313/1
type: Range
persistent identity: range2093313
display id: range2093313
version: 1
direction: direction/forward
top level: BBa_K404128
start: 2051
end: 2189
orientation: inline

mVenus
type: SequenceAnnotation
persistent identity: annotation2093312
display id: annotation2093312
version: 1
top level: BBa_K404128
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404128/annotation2093312/range2093312/1

https://synbiohub.org/public/igem/BBa_K404128/annotation2093312/range2093312/1
type: Range
persistent identity: range2093312
display id: range2093312
version: 1
direction: direction/forward
top level: BBa_K404128
start: 828
end: 1541
orientation: inline

CMV promter
type: SequenceAnnotation
persistent identity: annotation2093306
display id: annotation2093306
version: 1
top level: BBa_K404128
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K404128/annotation2093306/range2093306/1

https://synbiohub.org/public/igem/BBa_K404128/annotation2093306/range2093306/1
type: Range
persistent identity: range2093306
display id: range2093306
version: 1
direction: direction/forward
top level: BBa_K404128
start: 155
end: 809
orientation: inline

BBa_K404208
type: ComponentDefinition
persistent identity: BBa K404208
display id: BBa_K404208
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: ViralBrick-587-Beta Lactamase (BLA)
creator: Freiburg Bioware 2010
created: 2010-08-27T11:00:00Z
modified: 2015-05-08T01:12:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 7049
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: upstream 587
sequence annotation: downstream 453
sequence annotation: upstream 453
sequence annotation: Z34C motif
sequence annotation: downstream 587
sequence: https://synbiohub.org/public/igem/BBa_K404208_sequence/1

upstream 587
type: SequenceAnnotation
persistent identity: annotation2079112
display id: annotation2079112
version: 1
top level: BBa_K404208
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404208/annotation2079112/range2079112/1

https://synbiohub.org/public/igem/BBa_K404208/annotation2079112/range2079112/1
type: Range
persistent identity: range2079112
display id: range2079112
version: 1
direction: direction/forward
top level: BBa_K404208
start: 10
end: 42
orientation: inline

downstream 453
type: SequenceAnnotation
persistent identity: annotation2079115
display id: annotation2079115
version: 1
top level: BBa_K404208
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404208/annotation2079115/range2079115/1

https://synbiohub.org/public/igem/BBa_K404208/annotation2079115/range2079115/1
type: Range
persistent identity: range2079115
display id: range2079115
version: 1
direction: direction/forward
top level: BBa_K404208
start: 166
end: 174
orientation: inline

upstream 453
type: SequenceAnnotation
persistent identity: annotation2079111
display id: annotation2079111
version: 1
top level: BBa_K404208
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404208/annotation2079111/range2079111/1

https://synbiohub.org/public/igem/BBa_K404208/annotation2079111/range2079111/1
type: Range
persistent identity: range2079111
display id: range2079111
version: 1
direction: direction/forward
top level: BBa_K404208
start: 1
end: 9
orientation: inline

Z34C motif
type: SequenceAnnotation
persistent identity: annotation2079113
display id: annotation2079113
version: 1
top level: BBa_K404208
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404208/annotation2079113/range2079113/1

https://synbiohub.org/public/igem/BBa_K404208/annotation2079113/range2079113/1
type: Range
persistent identity: range2079113
display id: range2079113
version: 1
direction: direction/forward
top level: BBa_K404208
start: 43
end: 144
orientation: inline

downstream 587
type: SequenceAnnotation
persistent identity: annotation2079114
display id: annotation2079114
version: 1
top level: BBa_K404208
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404208/annotation2079114/range2079114/1

https://synbiohub.org/public/igem/BBa_K404208/annotation2079114/range2079114/1
type: Range
persistent identity: range2079114
display id: range2079114
version: 1
direction: direction/forward
top level: BBa_K404208
start: 145
end: 165
orientation: inline

BBa_K404157
type: ComponentDefinition
persistent identity: BBa K404157
display id: BBa_K404157
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pCMV_Z-EGFR-1907_Long-Linker_[AAV2]-VP23 (ViralBrick-587KO-Empty)
creator: Freiburg Bioware 2010
created: 2010-10-11T11:00:00Z
modified: 2015-05-08T01:12:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 6733
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: long-linker
sequence annotation: VP2
sequence annotation: VP3
sequence annotation: HSPG-KO
sequence annotation: ZEGFR_1907
sequence annotation: AAV-2-cap (Stratagene)
sequence annotation: CMV
sequence: https://synbiohub.org/public/igem/BBa_K404157_sequence/1

long-linker
type: SequenceAnnotation
persistent identity: annotation2094996
display id: annotation2094996
version: 1
top level: BBa_K404157
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404157/annotation2094996/range2094996/1

https://synbiohub.org/public/igem/BBa_K404157/annotation2094996/range2094996/1
type: Range
persistent identity: range2094996
display id: range2094996
version: 1
direction: direction/forward
top level: BBa_K404157
start: 851
end: 886
orientation: inline

VP2
type: SequenceAnnotation
persistent identity: annotation2094997
display id: annotation2094997
version: 1
top level: BBa_K404157
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404157/annotation2094997/range2094997/1

https://synbiohub.org/public/igem/BBa_K404157/annotation2094997/range2094997/1
type: Range
persistent identity: range2094997
display id: range2094997
version: 1
direction: direction/forward
top level: BBa_K404157
start: 893
end: 2686
orientation: inline

VP3
type: SequenceAnnotation
persistent identity: annotation2094998
display id: annotation2094998
version: 1
top level: BBa_K404157
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404157/annotation2094998/range2094998/1

https://synbiohub.org/public/igem/BBa_K404157/annotation2094998/range2094998/1
type: Range
persistent identity: range2094998
display id: range2094998
version: 1
direction: direction/forward
top level: BBa_K404157
start: 1085
end: 2686
orientation: inline

HSPG-KO
type: SequenceAnnotation
persistent identity: annotation2101792
display id: annotation2101792
version: 1
top level: BBa_K404157
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K404157/annotation2101792/range2101792/1

https://synbiohub.org/public/igem/BBa_K404157/annotation2101792/range2101792/1
type: Range
persistent identity: range2101792
display id: range2101792
version: 1
direction: direction/forward
top level: BBa_K404157
start: 2240
end: 2242
orientation: inline

ZEGFR_1907
type: SequenceAnnotation
persistent identity: annotation2094995
display id: annotation2094995
version: 1
top level: BBa_K404157
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404157/annotation2094995/range2094995/1

https://synbiohub.org/public/igem/BBa_K404157/annotation2094995/range2094995/1
type: Range
persistent identity: range2094995
display id: range2094995
version: 1
direction: direction/forward
top level: BBa_K404157
start: 671
end: 844
orientation: inline

AAV-2-cap (Stratagene)
type: SequenceAnnotation
persistent identity: annotation2094999
display id: annotation2094999
version: 1
top level: BBa_K404157
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404157/annotation2094999/range2094999/1

https://synbiohub.org/public/igem/BBa_K404157/annotation2094999/range2094999/1
type: Range
persistent identity: range2094999
display id: range2094999
version: 1
direction: direction/forward
top level: BBa_K404157
start: 893
end: 2809
orientation: inline

CMV
type: SequenceAnnotation
persistent identity: annotation2094994
display id: annotation2094994
version: 1
top level: BBa_K404157
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K404157/annotation2094994/range2094994/1

https://synbiohub.org/public/igem/BBa_K404157/annotation2094994/range2094994/1
type: Range
persistent identity: range2094994
display id: range2094994
version: 1
direction: direction/forward
top level: BBa_K404157
start: 2
end: 655
orientation: inline

HSPG-KO
type: SequenceAnnotation
persistent identity: annotation2101793
display id: annotation2101793
version: 1
top level: BBa_K404157
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K404157/annotation2101793/range2101793/1

https://synbiohub.org/public/igem/BBa_K404157/annotation2101793/range2101793/1
type: Range
persistent identity: range2101793
display id: range2101793
version: 1
direction: direction/forward
top level: BBa_K404157
start: 2231
end: 2233
orientation: inline

BBa_K404205
type: ComponentDefinition
persistent identity: BBa K404205
display id: BBa_K404205
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: ViralBrick-587-BAP
creator: Freiburg Bioware 2010
created: 2010-08-27T11:00:00Z
modified: 2015-05-08T01:12:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 7049
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: upstream 587
sequence annotation: downstream 587
sequence annotation: TG Spacer
sequence annotation: upstream 453
sequence annotation: GLS Spacer
sequence annotation: BAP-motif
sequence: https://synbiohub.org/public/igem/BBa_K404205_sequence/1

upstream 587
type: SequenceAnnotation
persistent identity: annotation2078948
display id: annotation2078948
version: 1
top level: BBa_K404205
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404205/annotation2078948/range2078948/1

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type: Range
persistent identity: range2078948
display id: range2078948
version: 1
direction: direction/forward
top level: BBa_K404205
start: 10
end: 42
orientation: inline

downstream 587
type: SequenceAnnotation
persistent identity: annotation2078953
display id: annotation2078953
version: 1
top level: BBa_K404205
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404205/annotation2078953/range2078953/1

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type: Range
persistent identity: range2078953
display id: range2078953
version: 1
direction: direction/forward
top level: BBa_K404205
start: 124
end: 132
orientation: inline

TG Spacer
type: SequenceAnnotation
persistent identity: annotation2078949
display id: annotation2078949
version: 1
top level: BBa_K404205
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404205/annotation2078949/range2078949/1

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type: Range
persistent identity: range2078949
display id: range2078949
version: 1
direction: direction/forward
top level: BBa_K404205
start: 43
end: 48
orientation: inline

downstream 587
type: SequenceAnnotation
persistent identity: annotation2078952
display id: annotation2078952
version: 1
top level: BBa_K404205
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404205/annotation2078952/range2078952/1

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type: Range
persistent identity: range2078952
display id: range2078952
version: 1
direction: direction/forward
top level: BBa_K404205
start: 103
end: 123
orientation: inline

upstream 453
type: SequenceAnnotation
persistent identity: annotation2078947
display id: annotation2078947
version: 1
top level: BBa_K404205
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404205/annotation2078947/range2078947/1

https://synbiohub.org/public/igem/BBa_K404205/annotation2078947/range2078947/1
type: Range
persistent identity: range2078947
display id: range2078947
version: 1
direction: direction/forward
top level: BBa_K404205
start: 1
end: 9
orientation: inline

GLS Spacer
type: SequenceAnnotation
persistent identity: annotation2078951
display id: annotation2078951
version: 1
top level: BBa_K404205
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404205/annotation2078951/range2078951/1

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type: Range
persistent identity: range2078951
display id: range2078951
version: 1
direction: direction/forward
top level: BBa_K404205
start: 94
end: 102
orientation: inline

BAP-motif
type: SequenceAnnotation
persistent identity: annotation2078950
display id: annotation2078950
version: 1
top level: BBa_K404205
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404205/annotation2078950/range2078950/1

https://synbiohub.org/public/igem/BBa_K404205/annotation2078950/range2078950/1
type: Range
persistent identity: range2078950
display id: range2078950
version: 1
direction: direction/forward
top level: BBa_K404205
start: 49
end: 93
orientation: inline

BBa_K404243
type: ComponentDefinition
persistent identity: BBa K404243
display id: BBa_K404243
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pCMV_[AAV2]VP123 (ViralBrick-453-RGD)
creator: Freiburg Bioware 2010
created: 2010-09-19T11:00:00Z
modified: 2015-05-08T01:12:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 7049
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: A-spacer
sequence annotation: upstream 453
sequence annotation: VP3
sequence annotation: CMV
sequence annotation: VP2
sequence annotation: VP1
sequence annotation: downstream 453
sequence annotation: RGD
sequence annotation: AAV-2-cap (Stratagene)
sequence: https://synbiohub.org/public/igem/BBa_K404243_sequence/1

A-spacer
type: SequenceAnnotation
persistent identity: annotation2094250
display id: annotation2094250
version: 1
top level: BBa_K404243
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404243/annotation2094250/range2094250/1

https://synbiohub.org/public/igem/BBa_K404243/annotation2094250/range2094250/1
type: Range
persistent identity: range2094250
display id: range2094250
version: 1
direction: direction/forward
top level: BBa_K404243
start: 2027
end: 2029
orientation: inline

upstream 453
type: SequenceAnnotation
persistent identity: annotation2094249
display id: annotation2094249
version: 1
top level: BBa_K404243
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404243/annotation2094249/range2094249/1

https://synbiohub.org/public/igem/BBa_K404243/annotation2094249/range2094249/1
type: Range
persistent identity: range2094249
display id: range2094249
version: 1
direction: direction/forward
top level: BBa_K404243
start: 1989
end: 2026
orientation: inline

VP3
type: SequenceAnnotation
persistent identity: annotation2094247
display id: annotation2094247
version: 1
top level: BBa_K404243
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404243/annotation2094247/range2094247/1

https://synbiohub.org/public/igem/BBa_K404243/annotation2094247/range2094247/1
type: Range
persistent identity: range2094247
display id: range2094247
version: 1
direction: direction/forward
top level: BBa_K404243
start: 1274
end: 2908
orientation: inline

CMV
type: SequenceAnnotation
persistent identity: annotation2094244
display id: annotation2094244
version: 1
top level: BBa_K404243
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K404243/annotation2094244/range2094244/1

https://synbiohub.org/public/igem/BBa_K404243/annotation2094244/range2094244/1
type: Range
persistent identity: range2094244
display id: range2094244
version: 1
direction: direction/forward
top level: BBa_K404243
start: 2
end: 655
orientation: inline

VP2
type: SequenceAnnotation
persistent identity: annotation2094246
display id: annotation2094246
version: 1
top level: BBa_K404243
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404243/annotation2094246/range2094246/1

https://synbiohub.org/public/igem/BBa_K404243/annotation2094246/range2094246/1
type: Range
persistent identity: range2094246
display id: range2094246
version: 1
direction: direction/forward
top level: BBa_K404243
start: 1079
end: 2908
orientation: inline

VP1
type: SequenceAnnotation
persistent identity: annotation2094245
display id: annotation2094245
version: 1
top level: BBa_K404243
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404243/annotation2094245/range2094245/1

https://synbiohub.org/public/igem/BBa_K404243/annotation2094245/range2094245/1
type: Range
persistent identity: range2094245
display id: range2094245
version: 1
direction: direction/forward
top level: BBa_K404243
start: 671
end: 2908
orientation: inline

downstream 453
type: SequenceAnnotation
persistent identity: annotation2094252
display id: annotation2094252
version: 1
top level: BBa_K404243
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404243/annotation2094252/range2094252/1

https://synbiohub.org/public/igem/BBa_K404243/annotation2094252/range2094252/1
type: Range
persistent identity: range2094252
display id: range2094252
version: 1
direction: direction/forward
top level: BBa_K404243
start: 2060
end: 2073
orientation: inline

A-spacer
type: SequenceAnnotation
persistent identity: annotation2094253
display id: annotation2094253
version: 1
top level: BBa_K404243
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404243/annotation2094253/range2094253/1

https://synbiohub.org/public/igem/BBa_K404243/annotation2094253/range2094253/1
type: Range
persistent identity: range2094253
display id: range2094253
version: 1
direction: direction/forward
top level: BBa_K404243
start: 2057
end: 2059
orientation: inline

RGD
type: SequenceAnnotation
persistent identity: annotation2094251
display id: annotation2094251
version: 1
top level: BBa_K404243
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404243/annotation2094251/range2094251/1

https://synbiohub.org/public/igem/BBa_K404243/annotation2094251/range2094251/1
type: Range
persistent identity: range2094251
display id: range2094251
version: 1
direction: direction/forward
top level: BBa_K404243
start: 2030
end: 2056
orientation: inline

AAV-2-cap (Stratagene)
type: SequenceAnnotation
persistent identity: annotation2094248
display id: annotation2094248
version: 1
top level: BBa_K404243
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404243/annotation2094248/range2094248/1

https://synbiohub.org/public/igem/BBa_K404243/annotation2094248/range2094248/1
type: Range
persistent identity: range2094248
display id: range2094248
version: 1
direction: direction/forward
top level: BBa_K404243
start: 1720
end: 3031
orientation: inline

BBa_K404156
type: ComponentDefinition
persistent identity: BBa K404156
display id: BBa_K404156
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pCMV_Z-EGFR-1907_Middle-Linker_[AAV2]-VP23 (ViralBrick-587KO-Empty)
creator: Freiburg Bioware 2010
created: 2010-10-11T11:00:00Z
modified: 2015-05-08T01:12:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 6733
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: VP3
sequence annotation: middle-linker
sequence annotation: CMV
sequence annotation: VP2
sequence annotation: HSPG-KO
sequence annotation: ZEGFR_1907
sequence annotation: AAV-2-cap (Stratagene)
sequence: https://synbiohub.org/public/igem/BBa_K404156_sequence/1

VP3
type: SequenceAnnotation
persistent identity: annotation2094981
display id: annotation2094981
version: 1
top level: BBa_K404156
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404156/annotation2094981/range2094981/1

https://synbiohub.org/public/igem/BBa_K404156/annotation2094981/range2094981/1
type: Range
persistent identity: range2094981
display id: range2094981
version: 1
direction: direction/forward
top level: BBa_K404156
start: 1073
end: 2674
orientation: inline

middle-linker
type: SequenceAnnotation
persistent identity: annotation2094992
display id: annotation2094992
version: 1
top level: BBa_K404156
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404156/annotation2094992/range2094992/1

https://synbiohub.org/public/igem/BBa_K404156/annotation2094992/range2094992/1
type: Range
persistent identity: range2094992
display id: range2094992
version: 1
direction: direction/forward
top level: BBa_K404156
start: 851
end: 874
orientation: inline

CMV
type: SequenceAnnotation
persistent identity: annotation2094979
display id: annotation2094979
version: 1
top level: BBa_K404156
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K404156/annotation2094979/range2094979/1

https://synbiohub.org/public/igem/BBa_K404156/annotation2094979/range2094979/1
type: Range
persistent identity: range2094979
display id: range2094979
version: 1
direction: direction/forward
top level: BBa_K404156
start: 2
end: 655
orientation: inline

VP2
type: SequenceAnnotation
persistent identity: annotation2094980
display id: annotation2094980
version: 1
top level: BBa_K404156
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404156/annotation2094980/range2094980/1

https://synbiohub.org/public/igem/BBa_K404156/annotation2094980/range2094980/1
type: Range
persistent identity: range2094980
display id: range2094980
version: 1
direction: direction/forward
top level: BBa_K404156
start: 881
end: 2674
orientation: inline

HSPG-KO
type: SequenceAnnotation
persistent identity: annotation2101755
display id: annotation2101755
version: 1
top level: BBa_K404156
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K404156/annotation2101755/range2101755/1

https://synbiohub.org/public/igem/BBa_K404156/annotation2101755/range2101755/1
type: Range
persistent identity: range2101755
display id: range2101755
version: 1
direction: direction/forward
top level: BBa_K404156
start: 2228
end: 2230
orientation: inline

ZEGFR_1907
type: SequenceAnnotation
persistent identity: annotation2094993
display id: annotation2094993
version: 1
top level: BBa_K404156
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404156/annotation2094993/range2094993/1

https://synbiohub.org/public/igem/BBa_K404156/annotation2094993/range2094993/1
type: Range
persistent identity: range2094993
display id: range2094993
version: 1
direction: direction/forward
top level: BBa_K404156
start: 671
end: 844
orientation: inline

AAV-2-cap (Stratagene)
type: SequenceAnnotation
persistent identity: annotation2094991
display id: annotation2094991
version: 1
top level: BBa_K404156
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404156/annotation2094991/range2094991/1

https://synbiohub.org/public/igem/BBa_K404156/annotation2094991/range2094991/1
type: Range
persistent identity: range2094991
display id: range2094991
version: 1
direction: direction/forward
top level: BBa_K404156
start: 881
end: 2797
orientation: inline

HSPG-KO
type: SequenceAnnotation
persistent identity: annotation2101782
display id: annotation2101782
version: 1
top level: BBa_K404156
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K404156/annotation2101782/range2101782/1

https://synbiohub.org/public/igem/BBa_K404156/annotation2101782/range2101782/1
type: Range
persistent identity: range2101782
display id: range2101782
version: 1
direction: direction/forward
top level: BBa_K404156
start: 2219
end: 2221
orientation: inline

BBa_K404158
type: ComponentDefinition
persistent identity: BBa K404158
display id: BBa_K404158
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pCMV_Z-EGFR-1907_SEG-Linker_[AAV2]-VP23 (ViralBrick-587KO-Empty)
creator: Freiburg Bioware 2010
created: 2010-10-11T11:00:00Z
modified: 2015-05-08T01:12:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 6733
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: AAV-2-cap (Stratagene)
sequence annotation: HSPG-KO
sequence annotation: ZEGFR_1907
sequence annotation: VP2
sequence annotation: CMV
sequence annotation: VP3
sequence annotation: SEG-linker
sequence: https://synbiohub.org/public/igem/BBa_K404158_sequence/1

AAV-2-cap (Stratagene)
type: SequenceAnnotation
persistent identity: annotation2095005
display id: annotation2095005
version: 1
top level: BBa_K404158
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404158/annotation2095005/range2095005/1

https://synbiohub.org/public/igem/BBa_K404158/annotation2095005/range2095005/1
type: Range
persistent identity: range2095005
display id: range2095005
version: 1
direction: direction/forward
top level: BBa_K404158
start: 965
end: 2881
orientation: inline

HSPG-KO
type: SequenceAnnotation
persistent identity: annotation2101795
display id: annotation2101795
version: 1
top level: BBa_K404158
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K404158/annotation2101795/range2101795/1

https://synbiohub.org/public/igem/BBa_K404158/annotation2101795/range2101795/1
type: Range
persistent identity: range2101795
display id: range2101795
version: 1
direction: direction/forward
top level: BBa_K404158
start: 2303
end: 2305
orientation: inline

HSPG-KO
type: SequenceAnnotation
persistent identity: annotation2101794
display id: annotation2101794
version: 1
top level: BBa_K404158
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K404158/annotation2101794/range2101794/1

https://synbiohub.org/public/igem/BBa_K404158/annotation2101794/range2101794/1
type: Range
persistent identity: range2101794
display id: range2101794
version: 1
direction: direction/forward
top level: BBa_K404158
start: 2312
end: 2314
orientation: inline

ZEGFR_1907
type: SequenceAnnotation
persistent identity: annotation2095001
display id: annotation2095001
version: 1
top level: BBa_K404158
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404158/annotation2095001/range2095001/1

https://synbiohub.org/public/igem/BBa_K404158/annotation2095001/range2095001/1
type: Range
persistent identity: range2095001
display id: range2095001
version: 1
direction: direction/forward
top level: BBa_K404158
start: 671
end: 844
orientation: inline

VP2
type: SequenceAnnotation
persistent identity: annotation2095003
display id: annotation2095003
version: 1
top level: BBa_K404158
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404158/annotation2095003/range2095003/1

https://synbiohub.org/public/igem/BBa_K404158/annotation2095003/range2095003/1
type: Range
persistent identity: range2095003
display id: range2095003
version: 1
direction: direction/forward
top level: BBa_K404158
start: 965
end: 2758
orientation: inline

CMV
type: SequenceAnnotation
persistent identity: annotation2095000
display id: annotation2095000
version: 1
top level: BBa_K404158
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K404158/annotation2095000/range2095000/1

https://synbiohub.org/public/igem/BBa_K404158/annotation2095000/range2095000/1
type: Range
persistent identity: range2095000
display id: range2095000
version: 1
direction: direction/forward
top level: BBa_K404158
start: 2
end: 655
orientation: inline

VP3
type: SequenceAnnotation
persistent identity: annotation2095004
display id: annotation2095004
version: 1
top level: BBa_K404158
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404158/annotation2095004/range2095004/1

https://synbiohub.org/public/igem/BBa_K404158/annotation2095004/range2095004/1
type: Range
persistent identity: range2095004
display id: range2095004
version: 1
direction: direction/forward
top level: BBa_K404158
start: 1157
end: 2758
orientation: inline

SEG-linker
type: SequenceAnnotation
persistent identity: annotation2095002
display id: annotation2095002
version: 1
top level: BBa_K404158
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404158/annotation2095002/range2095002/1

https://synbiohub.org/public/igem/BBa_K404158/annotation2095002/range2095002/1
type: Range
persistent identity: range2095002
display id: range2095002
version: 1
direction: direction/forward
top level: BBa_K404158
start: 851
end: 858
orientation: inline

BBa_K1926021
type: ComponentDefinition
persistent identity: BBa K1926021
display id: BBa_K1926021
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: The SNAP-VIKA UNIT
creator: Xinyi Liu
created: 2016-10-09T11:00:00Z
modified: 2016-10-11T06:21:46Z
discontinued: false
dominant: false
group u list: _2393_
m user id: 28998
owner id: 28998
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This part is the SNAP-VIKA UNIT of cyclebow including a SNAP UNIT and a VIKA UNIT. The SNAP UNIT, which can be cut off by CRE, includes an sv40 nuclear location sequence (NLS), a covalent labeling of fusion proteins named SNAP-tag?? and an sv40 terminator flanked by homodromous recognition site of recombinase CRE named loxP. The VIKA UNIT, which can be cut off by VIKA, includes an sv40 nuclear location sequence (NLS), a recombinase gene named VIKA and an sv40 terminator flanked by homodromous recognition site of recombinase VIKA named Vox. You may use this part to: 1) Test the cut-off efficiency of recombinase CRE and VIKA by qRT-PCR; 2) Estimate the time needed for genome repair because VIKA can only be expressed after the cut off of SNAP UNIT and genome repair; 3) Fast cut off this part by transient transfecting recombinase gene into nucleus; 4) When there is no CRE, you can use this part as the SNAP UNIT, which can help localize proteins in vivo with BLOCK and dye from NEB company.
mutable notes: None
mutable provenance: The SNAP-tag?? was brought from NEB company. NLS and sv40 terminator were got from commercial plasmid: pentry nls GFP/pcdna3.1. The recombinase gene VIKA and its RTS Vox were got through denovo synthesis.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1926012
component: BBa_K1926011
sequence annotation: BBa_K1926011
sequence annotation: BBa_K1926012
sequence: https://synbiohub.org/public/igem/BBa_K1926021_sequence/1

BBa_K1926012
type: Component
persistent identity: component2491919
display id: component2491919
version: 1
top level: BBa_K1926021
access: public
definition: BBa_K1926012

BBa_K1926011
type: Component
persistent identity: component2491913
display id: component2491913
version: 1
top level: BBa_K1926021
access: public
definition: BBa_K1926011

BBa_K1926011
type: SequenceAnnotation
persistent identity: annotation2491913
display id: annotation2491913
version: 1
top level: BBa_K1926021
location: https://synbiohub.org/public/igem/BBa_K1926021/annotation2491913/range2491913/1
component: BBa_K1926011

https://synbiohub.org/public/igem/BBa_K1926021/annotation2491913/range2491913/1
type: Range
persistent identity: range2491913
display id: range2491913
version: 1
direction: direction/reverse
top level: BBa_K1926021
start: 1
end: 788
orientation: inline

BBa_K1926012
type: SequenceAnnotation
persistent identity: annotation2491919
display id: annotation2491919
version: 1
top level: BBa_K1926021
location: https://synbiohub.org/public/igem/BBa_K1926021/annotation2491919/range2491919/1
component: BBa_K1926012

https://synbiohub.org/public/igem/BBa_K1926021/annotation2491919/range2491919/1
type: Range
persistent identity: range2491919
display id: range2491919
version: 1
direction: direction/reverse
top level: BBa_K1926021
start: 789
end: 2082
orientation: inline

BBa_K404251
type: ComponentDefinition
persistent identity: BBa K404251
display id: BBa_K404251
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pCMV_[AAV2]-VP123ex (ViralBrick-587KO-BAP)
creator: Freiburg Bioware 2010
created: 2010-10-13T11:00:00Z
modified: 2015-05-08T01:12:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 7049
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: downstream 587
sequence annotation: TG-spacer
sequence annotation: HSPG-KO
sequence annotation: CMV promoter
sequence annotation: VP2
sequence annotation: VP3
sequence annotation: AAV-2-cap (Stratagene)
sequence annotation: VP1
sequence annotation: BAP
sequence annotation: GLS-spacer
sequence annotation: upstream 587
sequence: https://synbiohub.org/public/igem/BBa_K404251_sequence/1

downstream 587
type: SequenceAnnotation
persistent identity: annotation2094530
display id: annotation2094530
version: 1
top level: BBa_K404251
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404251/annotation2094530/range2094530/1

https://synbiohub.org/public/igem/BBa_K404251/annotation2094530/range2094530/1
type: Range
persistent identity: range2094530
display id: range2094530
version: 1
direction: direction/forward
top level: BBa_K404251
start: 2489
end: 2504
orientation: inline

TG-spacer
type: SequenceAnnotation
persistent identity: annotation2094527
display id: annotation2094527
version: 1
top level: BBa_K404251
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404251/annotation2094527/range2094527/1

https://synbiohub.org/public/igem/BBa_K404251/annotation2094527/range2094527/1
type: Range
persistent identity: range2094527
display id: range2094527
version: 1
direction: direction/forward
top level: BBa_K404251
start: 2429
end: 2434
orientation: inline

HSPG-KO
type: SequenceAnnotation
persistent identity: annotation2101876
display id: annotation2101876
version: 1
top level: BBa_K404251
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K404251/annotation2101876/range2101876/1

https://synbiohub.org/public/igem/BBa_K404251/annotation2101876/range2101876/1
type: Range
persistent identity: range2101876
display id: range2101876
version: 1
direction: direction/forward
top level: BBa_K404251
start: 2419
end: 2421
orientation: inline

CMV promoter
type: SequenceAnnotation
persistent identity: annotation2094521
display id: annotation2094521
version: 1
top level: BBa_K404251
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K404251/annotation2094521/range2094521/1

https://synbiohub.org/public/igem/BBa_K404251/annotation2094521/range2094521/1
type: Range
persistent identity: range2094521
display id: range2094521
version: 1
direction: direction/forward
top level: BBa_K404251
start: 2
end: 655
orientation: inline

VP2
type: SequenceAnnotation
persistent identity: annotation2094524
display id: annotation2094524
version: 1
top level: BBa_K404251
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404251/annotation2094524/range2094524/1

https://synbiohub.org/public/igem/BBa_K404251/annotation2094524/range2094524/1
type: Range
persistent identity: range2094524
display id: range2094524
version: 1
direction: direction/forward
top level: BBa_K404251
start: 1079
end: 2935
orientation: inline

VP3
type: SequenceAnnotation
persistent identity: annotation2094525
display id: annotation2094525
version: 1
top level: BBa_K404251
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404251/annotation2094525/range2094525/1

https://synbiohub.org/public/igem/BBa_K404251/annotation2094525/range2094525/1
type: Range
persistent identity: range2094525
display id: range2094525
version: 1
direction: direction/forward
top level: BBa_K404251
start: 1274
end: 2935
orientation: inline

AAV-2-cap (Stratagene)
type: SequenceAnnotation
persistent identity: annotation2094523
display id: annotation2094523
version: 1
top level: BBa_K404251
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404251/annotation2094523/range2094523/1

https://synbiohub.org/public/igem/BBa_K404251/annotation2094523/range2094523/1
type: Range
persistent identity: range2094523
display id: range2094523
version: 1
direction: direction/forward
top level: BBa_K404251
start: 671
end: 3058
orientation: inline

HSPG-KO
type: SequenceAnnotation
persistent identity: annotation2101875
display id: annotation2101875
version: 1
top level: BBa_K404251
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K404251/annotation2101875/range2101875/1

https://synbiohub.org/public/igem/BBa_K404251/annotation2101875/range2101875/1
type: Range
persistent identity: range2101875
display id: range2101875
version: 1
direction: direction/forward
top level: BBa_K404251
start: 2481
end: 2483
orientation: inline

VP1
type: SequenceAnnotation
persistent identity: annotation2094522
display id: annotation2094522
version: 1
top level: BBa_K404251
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404251/annotation2094522/range2094522/1

https://synbiohub.org/public/igem/BBa_K404251/annotation2094522/range2094522/1
type: Range
persistent identity: range2094522
display id: range2094522
version: 1
direction: direction/forward
top level: BBa_K404251
start: 671
end: 2935
orientation: inline

BAP
type: SequenceAnnotation
persistent identity: annotation2094528
display id: annotation2094528
version: 1
top level: BBa_K404251
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404251/annotation2094528/range2094528/1

https://synbiohub.org/public/igem/BBa_K404251/annotation2094528/range2094528/1
type: Range
persistent identity: range2094528
display id: range2094528
version: 1
direction: direction/forward
top level: BBa_K404251
start: 2435
end: 2479
orientation: inline

GLS-spacer
type: SequenceAnnotation
persistent identity: annotation2094529
display id: annotation2094529
version: 1
top level: BBa_K404251
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404251/annotation2094529/range2094529/1

https://synbiohub.org/public/igem/BBa_K404251/annotation2094529/range2094529/1
type: Range
persistent identity: range2094529
display id: range2094529
version: 1
direction: direction/forward
top level: BBa_K404251
start: 2480
end: 2488
orientation: inline

upstream 587
type: SequenceAnnotation
persistent identity: annotation2094526
display id: annotation2094526
version: 1
top level: BBa_K404251
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404251/annotation2094526/range2094526/1

https://synbiohub.org/public/igem/BBa_K404251/annotation2094526/range2094526/1
type: Range
persistent identity: range2094526
display id: range2094526
version: 1
direction: direction/forward
top level: BBa_K404251
start: 2397
end: 2428
orientation: inline

BBa_K2061000
type: ComponentDefinition
persistent identity: BBa K2061000
display id: BBa_K2061000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: tGFP ORF under EF1a promoter
creator: avi matityahu
created: 2016-08-22T11:00:00Z
modified: 2016-10-19T12:38:45Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2529_
m user id: 30576
owner id: 31098
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This plasmid contains the tGFP open reading frame under the control of human EF1a promoter. The part was cloned into the pSB1C3 plasmid with chloramphenicol rresistance.
mutable notes: the part cloned between EcoRI and XbaI sites.
mutable provenance: synthetic production of the part
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000155
role: partType/Plasmid
sequence annotation: EcoRI site
sequence annotation: EF1a promoter
sequence annotation: stop codon
sequence annotation: XbaI site
sequence annotation: start codon
sequence annotation: tGFP
sequence: https://synbiohub.org/public/igem/BBa_K2061000_sequence/1

EcoRI site
type: SequenceAnnotation
persistent identity: annotation2481534
display id: annotation2481534
version: 1
top level: BBa_K2061000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K2061000/annotation2481534/range2481534/1

https://synbiohub.org/public/igem/BBa_K2061000/annotation2481534/range2481534/1
type: Range
persistent identity: range2481534
display id: range2481534
version: 1
direction: direction/forward
top level: BBa_K2061000
start: 1
end: 6
orientation: inline

EF1a promoter
type: SequenceAnnotation
persistent identity: annotation2481530
display id: annotation2481530
version: 1
top level: BBa_K2061000
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K2061000/annotation2481530/range2481530/1

https://synbiohub.org/public/igem/BBa_K2061000/annotation2481530/range2481530/1
type: Range
persistent identity: range2481530
display id: range2481530
version: 1
direction: direction/forward
top level: BBa_K2061000
start: 6
end: 1155
orientation: inline

stop codon
type: SequenceAnnotation
persistent identity: annotation2481533
display id: annotation2481533
version: 1
top level: BBa_K2061000
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K2061000/annotation2481533/range2481533/1

https://synbiohub.org/public/igem/BBa_K2061000/annotation2481533/range2481533/1
type: Range
persistent identity: range2481533
display id: range2481533
version: 1
direction: direction/forward
top level: BBa_K2061000
start: 1948
end: 1950
orientation: inline

XbaI site
type: SequenceAnnotation
persistent identity: annotation2481535
display id: annotation2481535
version: 1
top level: BBa_K2061000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K2061000/annotation2481535/range2481535/1

https://synbiohub.org/public/igem/BBa_K2061000/annotation2481535/range2481535/1
type: Range
persistent identity: range2481535
display id: range2481535
version: 1
direction: direction/forward
top level: BBa_K2061000
start: 1985
end: 1990
orientation: inline

start codon
type: SequenceAnnotation
persistent identity: annotation2481532
display id: annotation2481532
version: 1
top level: BBa_K2061000
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K2061000/annotation2481532/range2481532/1

https://synbiohub.org/public/igem/BBa_K2061000/annotation2481532/range2481532/1
type: Range
persistent identity: range2481532
display id: range2481532
version: 1
direction: direction/forward
top level: BBa_K2061000
start: 1192
end: 1194
orientation: inline

tGFP
type: SequenceAnnotation
persistent identity: annotation2481531
display id: annotation2481531
version: 1
top level: BBa_K2061000
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K2061000/annotation2481531/range2481531/1

https://synbiohub.org/public/igem/BBa_K2061000/annotation2481531/range2481531/1
type: Range
persistent identity: range2481531
display id: range2481531
version: 1
direction: direction/forward
top level: BBa_K2061000
start: 1192
end: 1950
orientation: inline

BBa_K404103
type: ComponentDefinition
persistent identity: BBa K404103
display id: BBa_K404103
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: p5 promoter
creator: Freiburg Bioware 2010
created: 2010-08-27T11:00:00Z
modified: 2015-05-08T01:12:23Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 7049
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: p5 promoter
sequence: https://synbiohub.org/public/igem/BBa_K404103_sequence/1

p5 promoter
type: SequenceAnnotation
persistent identity: annotation2093169
display id: annotation2093169
version: 1
top level: BBa_K404103
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K404103/annotation2093169/range2093169/1

https://synbiohub.org/public/igem/BBa_K404103/annotation2093169/range2093169/1
type: Range
persistent identity: range2093169
display id: range2093169
version: 1
direction: direction/forward
top level: BBa_K404103
start: 1
end: 166
orientation: inline

BBa_I712020
type: ComponentDefinition
persistent identity: BBa I712020
display id: BBa_I712020
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Homo sapiens caspase 3
creator: Anja Korenčič
created: 2007-10-20T11:00:00Z
modified: 2015-08-31T04:07:46Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _130_
m user id: 0
owner id: 1846
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Homo sapiens caspase 3, apoptosis-related cysteine peptidase (transcript variant beta).
mutable notes: Includes start and stop codon. The part is cloned into BBa_J52017, a vector with eukaryotic terminator.
mutable provenance: Part comes from Origene SC101958 vector (NM_032991).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_I712020_sequence/1

BBa_K404009
type: ComponentDefinition
persistent identity: BBa K404009
display id: BBa_K404009
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pCMV_[AAV2]-VP1
creator: Freiburg Bioware 2010
created: 2010-10-20T11:00:00Z
modified: 2015-05-08T01:12:23Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 6674
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: VP1
sequence annotation: CMV
sequence: https://synbiohub.org/public/igem/BBa_K404009_sequence/1

VP1
type: SequenceAnnotation
persistent identity: annotation2097902
display id: annotation2097902
version: 1
top level: BBa_K404009
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404009/annotation2097902/range2097902/1

https://synbiohub.org/public/igem/BBa_K404009/annotation2097902/range2097902/1
type: Range
persistent identity: range2097902
display id: range2097902
version: 1
direction: direction/forward
top level: BBa_K404009
start: 670
end: 2910
orientation: inline

CMV
type: SequenceAnnotation
persistent identity: annotation2097901
display id: annotation2097901
version: 1
top level: BBa_K404009
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K404009/annotation2097901/range2097901/1

https://synbiohub.org/public/igem/BBa_K404009/annotation2097901/range2097901/1
type: Range
persistent identity: range2097901
display id: range2097901
version: 1
direction: direction/forward
top level: BBa_K404009
start: 2
end: 655
orientation: inline

BBa_K1936001
type: ComponentDefinition
persistent identity: BBa K1936001
display id: BBa_K1936001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Tom5-eGFP-LOV2
creator: Marcel Dickmanns
created: 2016-10-12T11:00:00Z
modified: 2016-10-19T03:03:02Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2403_
m user id: 30478
owner id: 31678
owning group id: 9
status: status/Planning
bookmark: true
mutable description: ALERT !!!Under construction !!
mutable notes: ALERT !!!Under construction !!
mutable provenance: ALERT !!!Under construction !!
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Other
role: SO:0000110
sequence annotation: LOV2
sequence annotation: TOM5
sequence annotation: Linker
sequence annotation: eGFP
sequence: https://synbiohub.org/public/igem/BBa_K1936001_sequence/1

LOV2
type: SequenceAnnotation
persistent identity: annotation2521541
display id: annotation2521541
version: 1
top level: BBa_K1936001
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1936001/annotation2521541/range2521541/1

https://synbiohub.org/public/igem/BBa_K1936001/annotation2521541/range2521541/1
type: Range
persistent identity: range2521541
display id: range2521541
version: 1
direction: direction/forward
top level: BBa_K1936001
start: 937
end: 1368
orientation: inline

TOM5
type: SequenceAnnotation
persistent identity: annotation2521537
display id: annotation2521537
version: 1
top level: BBa_K1936001
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1936001/annotation2521537/range2521537/1

https://synbiohub.org/public/igem/BBa_K1936001/annotation2521537/range2521537/1
type: Range
persistent identity: range2521537
display id: range2521537
version: 1
direction: direction/forward
top level: BBa_K1936001
start: 1
end: 153
orientation: inline

Linker
type: SequenceAnnotation
persistent identity: annotation2521540
display id: annotation2521540
version: 1
top level: BBa_K1936001
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1936001/annotation2521540/range2521540/1

https://synbiohub.org/public/igem/BBa_K1936001/annotation2521540/range2521540/1
type: Range
persistent identity: range2521540
display id: range2521540
version: 1
direction: direction/forward
top level: BBa_K1936001
start: 907
end: 936
orientation: inline

Linker
type: SequenceAnnotation
persistent identity: annotation2521538
display id: annotation2521538
version: 1
top level: BBa_K1936001
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1936001/annotation2521538/range2521538/1

https://synbiohub.org/public/igem/BBa_K1936001/annotation2521538/range2521538/1
type: Range
persistent identity: range2521538
display id: range2521538
version: 1
direction: direction/forward
top level: BBa_K1936001
start: 154
end: 192
orientation: inline

eGFP
type: SequenceAnnotation
persistent identity: annotation2521539
display id: annotation2521539
version: 1
top level: BBa_K1936001
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1936001/annotation2521539/range2521539/1

https://synbiohub.org/public/igem/BBa_K1936001/annotation2521539/range2521539/1
type: Range
persistent identity: range2521539
display id: range2521539
version: 1
direction: direction/forward
top level: BBa_K1936001
start: 193
end: 906
orientation: inline

BBa_K404223
type: ComponentDefinition
persistent identity: BBa K404223
display id: BBa_K404223
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: [AAV2]-Rep-VP123_p5-TATAless (ViralBrick-453-RGD)
creator: Freiburg Bioware 2010
created: 2010-08-27T11:00:00Z
modified: 2015-05-08T01:12:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 7049
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: VP3
sequence annotation: A-spacer
sequence annotation: Rep78
sequence annotation: VP2
sequence annotation: p5TATAless
sequence annotation: Rep40
sequence annotation: VP1
sequence annotation: RGD
sequence annotation: Rep68
sequence annotation: Rep52
sequence: https://synbiohub.org/public/igem/BBa_K404223_sequence/1

VP3
type: SequenceAnnotation
persistent identity: annotation2098396
display id: annotation2098396
version: 1
top level: BBa_K404223
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404223/annotation2098396/range2098396/1

https://synbiohub.org/public/igem/BBa_K404223/annotation2098396/range2098396/1
type: Range
persistent identity: range2098396
display id: range2098396
version: 1
direction: direction/forward
top level: BBa_K404223
start: 2489
end: 4123
orientation: inline

A-spacer
type: SequenceAnnotation
persistent identity: annotation2098400
display id: annotation2098400
version: 1
top level: BBa_K404223
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404223/annotation2098400/range2098400/1

https://synbiohub.org/public/igem/BBa_K404223/annotation2098400/range2098400/1
type: Range
persistent identity: range2098400
display id: range2098400
version: 1
direction: direction/forward
top level: BBa_K404223
start: 3272
end: 3274
orientation: inline

Rep78
type: SequenceAnnotation
persistent identity: annotation2098338
display id: annotation2098338
version: 1
top level: BBa_K404223
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404223/annotation2098338/range2098338/1

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type: Range
persistent identity: range2098338
display id: range2098338
version: 1
direction: direction/forward
top level: BBa_K404223
start: 2
end: 1867
orientation: inline

VP2
type: SequenceAnnotation
persistent identity: annotation2098370
display id: annotation2098370
version: 1
top level: BBa_K404223
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404223/annotation2098370/range2098370/1

https://synbiohub.org/public/igem/BBa_K404223/annotation2098370/range2098370/1
type: Range
persistent identity: range2098370
display id: range2098370
version: 1
direction: direction/forward
top level: BBa_K404223
start: 2294
end: 4123
orientation: inline

p5TATAless
type: SequenceAnnotation
persistent identity: annotation2098397
display id: annotation2098397
version: 1
top level: BBa_K404223
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404223/annotation2098397/range2098397/1

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type: Range
persistent identity: range2098397
display id: range2098397
version: 1
direction: direction/forward
top level: BBa_K404223
start: 4255
end: 4418
orientation: inline

Rep40
type: SequenceAnnotation
persistent identity: annotation2098341
display id: annotation2098341
version: 1
top level: BBa_K404223
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404223/annotation2098341/range2098341/1

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type: Range
persistent identity: range2098341
display id: range2098341
version: 1
direction: direction/forward
top level: BBa_K404223
start: 673
end: 1933
orientation: inline

VP1
type: SequenceAnnotation
persistent identity: annotation2098369
display id: annotation2098369
version: 1
top level: BBa_K404223
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404223/annotation2098369/range2098369/1

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type: Range
persistent identity: range2098369
display id: range2098369
version: 1
direction: direction/forward
top level: BBa_K404223
start: 1883
end: 4123
orientation: inline

RGD
type: SequenceAnnotation
persistent identity: annotation2098398
display id: annotation2098398
version: 1
top level: BBa_K404223
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404223/annotation2098398/range2098398/1

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type: Range
persistent identity: range2098398
display id: range2098398
version: 1
direction: direction/forward
top level: BBa_K404223
start: 3245
end: 3271
orientation: inline

Rep68
type: SequenceAnnotation
persistent identity: annotation2098340
display id: annotation2098340
version: 1
top level: BBa_K404223
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404223/annotation2098340/range2098340/1

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type: Range
persistent identity: range2098340
display id: range2098340
version: 1
direction: direction/forward
top level: BBa_K404223
start: 2
end: 1933
orientation: inline

Rep52
type: SequenceAnnotation
persistent identity: annotation2098342
display id: annotation2098342
version: 1
top level: BBa_K404223
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404223/annotation2098342/range2098342/1

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type: Range
persistent identity: range2098342
display id: range2098342
version: 1
direction: direction/forward
top level: BBa_K404223
start: 673
end: 1867
orientation: inline

A-spacer
type: SequenceAnnotation
persistent identity: annotation2098399
display id: annotation2098399
version: 1
top level: BBa_K404223
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404223/annotation2098399/range2098399/1

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type: Range
persistent identity: range2098399
display id: range2098399
version: 1
direction: direction/forward
top level: BBa_K404223
start: 3242
end: 3244
orientation: inline

BBa_K258002
type: ComponentDefinition
persistent identity: BBa K258002
display id: BBa_K258002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Granulysin, a T Cell Product,Kills Bacteria by Altering Membrane Permeability
creator: Ozkan IS
created: 2009-10-02T11:00:00Z
modified: 2016-02-01T12:59:26Z
discontinued: false
dominant: true
experience: experience/None
group u list: _358_
m user id: 4206
owner id: 4260
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: Granulysin, a protein located in the acidic granules of human NK cells and cytotoxic T cells, has microbial activity against a broad spectrum of microbial pathogens. The substance released by cytotoxic T cells (CD8) when attached to infected body cells. It creates holes in the target cell membrane and destroy it. That is,Granulysin induces apoptosis in target cells and have antimicrobial action. It is broadly antimicrobial, killing microbes that cause, for example, tuberculosis and malaria, and can destroy some tumors. A series of peptides generated from the amino acid sequence of granulysin are potential antibiotics. Granulysin increased the permeability of bacterial membranes, as judged by its ability to allow access of cytosolic ??-galactosidase to its impermeant substrate.Granulysin functions to create holes in the target cell membrane and destroy it. Granulysin is able to induce apoptosis in target cells and also has antimicrobial action.
mutable notes: Granulysin can be purified via nickel affinity chromatography.
mutable provenance: Granulysin is a member of the saposin-like protein (SAPLIP) family. The gene was synthesized at GENEART in pMA vector
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K258002_sequence/1

BBa_K1936000
type: ComponentDefinition
persistent identity: BBa K1936000
display id: BBa_K1936000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: ePDZ-mCherry-BAX184E
creator: Marcel Dickmanns
created: 2016-10-12T11:00:00Z
modified: 2016-10-19T03:03:53Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2403_
m user id: 30478
owner id: 31678
owning group id: 9
status: status/Planning
bookmark: true
mutable description: ALERT !!!Under construction !!
mutable notes: ALERT !!!Under construction !!
mutable provenance: ALERT !!!Under construction !!
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Other
role: SO:0000110
sequence annotation: mCherry
sequence annotation: Linker
sequence annotation: ePDZ
sequence annotation: BaxS184e
sequence: https://synbiohub.org/public/igem/BBa_K1936000_sequence/1

mCherry
type: SequenceAnnotation
persistent identity: annotation2521534
display id: annotation2521534
version: 1
top level: BBa_K1936000
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1936000/annotation2521534/range2521534/1

https://synbiohub.org/public/igem/BBa_K1936000/annotation2521534/range2521534/1
type: Range
persistent identity: range2521534
display id: range2521534
version: 1
direction: direction/forward
top level: BBa_K1936000
start: 610
end: 1317
orientation: inline

Linker
type: SequenceAnnotation
persistent identity: annotation2521532
display id: annotation2521532
version: 1
top level: BBa_K1936000
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1936000/annotation2521532/range2521532/1

https://synbiohub.org/public/igem/BBa_K1936000/annotation2521532/range2521532/1
type: Range
persistent identity: range2521532
display id: range2521532
version: 1
direction: direction/forward
top level: BBa_K1936000
start: 583
end: 609
orientation: inline

Linker
type: SequenceAnnotation
persistent identity: annotation2521535
display id: annotation2521535
version: 1
top level: BBa_K1936000
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1936000/annotation2521535/range2521535/1

https://synbiohub.org/public/igem/BBa_K1936000/annotation2521535/range2521535/1
type: Range
persistent identity: range2521535
display id: range2521535
version: 1
direction: direction/forward
top level: BBa_K1936000
start: 1318
end: 1344
orientation: inline

ePDZ
type: SequenceAnnotation
persistent identity: annotation2521533
display id: annotation2521533
version: 1
top level: BBa_K1936000
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1936000/annotation2521533/range2521533/1

https://synbiohub.org/public/igem/BBa_K1936000/annotation2521533/range2521533/1
type: Range
persistent identity: range2521533
display id: range2521533
version: 1
direction: direction/forward
top level: BBa_K1936000
start: 1
end: 582
orientation: inline

BaxS184e
type: SequenceAnnotation
persistent identity: annotation2521536
display id: annotation2521536
version: 1
top level: BBa_K1936000
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1936000/annotation2521536/range2521536/1

https://synbiohub.org/public/igem/BBa_K1936000/annotation2521536/range2521536/1
type: Range
persistent identity: range2521536
display id: range2521536
version: 1
direction: direction/forward
top level: BBa_K1936000
start: 1345
end: 1920
orientation: inline

BBa_K404092
type: ComponentDefinition
persistent identity: BBa K404092
display id: BBa_K404092
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: [AAV2]-Rep68ex
creator: Freiburg Bioware 2010
created: 2010-08-27T11:00:00Z
modified: 2015-05-08T01:12:23Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 7049
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: Rep68
sequence: https://synbiohub.org/public/igem/BBa_K404092_sequence/1

Rep68
type: SequenceAnnotation
persistent identity: annotation2093162
display id: annotation2093162
version: 1
top level: BBa_K404092
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404092/annotation2093162/range2093162/1

https://synbiohub.org/public/igem/BBa_K404092/annotation2093162/range2093162/1
type: Range
persistent identity: range2093162
display id: range2093162
version: 1
direction: direction/forward
top level: BBa_K404092
start: 1
end: 1612
orientation: inline

BBa_K404152
type: ComponentDefinition
persistent identity: BBa K404152
display id: BBa_K404152
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: [AAV2]-VP1up
creator: Freiburg Bioware 2010
created: 2010-10-10T11:00:00Z
modified: 2015-05-08T01:12:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 6733
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: VP1up
sequence: https://synbiohub.org/public/igem/BBa_K404152_sequence/1

VP1up
type: SequenceAnnotation
persistent identity: annotation2093343
display id: annotation2093343
version: 1
top level: BBa_K404152
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404152/annotation2093343/range2093343/1

https://synbiohub.org/public/igem/BBa_K404152/annotation2093343/range2093343/1
type: Range
persistent identity: range2093343
display id: range2093343
version: 1
direction: direction/forward
top level: BBa_K404152
start: 1
end: 411
orientation: inline

BBa_K404207
type: ComponentDefinition
persistent identity: BBa K404207
display id: BBa_K404207
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: ViralBrick-587-RGD
creator: Freiburg Bioware 2010
created: 2010-08-27T11:00:00Z
modified: 2015-05-08T01:12:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 7049
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: GLS Spacer
sequence annotation: downstream 587
sequence annotation: upstream 587
sequence annotation: RGD-motif
sequence annotation: upstream 453
sequence annotation: TG Spacer
sequence: https://synbiohub.org/public/igem/BBa_K404207_sequence/1

GLS Spacer
type: SequenceAnnotation
persistent identity: annotation2079108
display id: annotation2079108
version: 1
top level: BBa_K404207
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404207/annotation2079108/range2079108/1

https://synbiohub.org/public/igem/BBa_K404207/annotation2079108/range2079108/1
type: Range
persistent identity: range2079108
display id: range2079108
version: 1
direction: direction/forward
top level: BBa_K404207
start: 76
end: 84
orientation: inline

downstream 587
type: SequenceAnnotation
persistent identity: annotation2079110
display id: annotation2079110
version: 1
top level: BBa_K404207
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404207/annotation2079110/range2079110/1

https://synbiohub.org/public/igem/BBa_K404207/annotation2079110/range2079110/1
type: Range
persistent identity: range2079110
display id: range2079110
version: 1
direction: direction/forward
top level: BBa_K404207
start: 106
end: 114
orientation: inline

downstream 587
type: SequenceAnnotation
persistent identity: annotation2079109
display id: annotation2079109
version: 1
top level: BBa_K404207
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404207/annotation2079109/range2079109/1

https://synbiohub.org/public/igem/BBa_K404207/annotation2079109/range2079109/1
type: Range
persistent identity: range2079109
display id: range2079109
version: 1
direction: direction/forward
top level: BBa_K404207
start: 85
end: 105
orientation: inline

upstream 587
type: SequenceAnnotation
persistent identity: annotation2079105
display id: annotation2079105
version: 1
top level: BBa_K404207
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404207/annotation2079105/range2079105/1

https://synbiohub.org/public/igem/BBa_K404207/annotation2079105/range2079105/1
type: Range
persistent identity: range2079105
display id: range2079105
version: 1
direction: direction/forward
top level: BBa_K404207
start: 10
end: 42
orientation: inline

RGD-motif
type: SequenceAnnotation
persistent identity: annotation2079107
display id: annotation2079107
version: 1
top level: BBa_K404207
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404207/annotation2079107/range2079107/1

https://synbiohub.org/public/igem/BBa_K404207/annotation2079107/range2079107/1
type: Range
persistent identity: range2079107
display id: range2079107
version: 1
direction: direction/forward
top level: BBa_K404207
start: 49
end: 75
orientation: inline

upstream 453
type: SequenceAnnotation
persistent identity: annotation2079104
display id: annotation2079104
version: 1
top level: BBa_K404207
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404207/annotation2079104/range2079104/1

https://synbiohub.org/public/igem/BBa_K404207/annotation2079104/range2079104/1
type: Range
persistent identity: range2079104
display id: range2079104
version: 1
direction: direction/forward
top level: BBa_K404207
start: 1
end: 9
orientation: inline

TG Spacer
type: SequenceAnnotation
persistent identity: annotation2079106
display id: annotation2079106
version: 1
top level: BBa_K404207
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404207/annotation2079106/range2079106/1

https://synbiohub.org/public/igem/BBa_K404207/annotation2079106/range2079106/1
type: Range
persistent identity: range2079106
display id: range2079106
version: 1
direction: direction/forward
top level: BBa_K404207
start: 43
end: 48
orientation: inline

BBa_K404234
type: ComponentDefinition
persistent identity: BBa K404234
display id: BBa_K404234
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: [AAV2]-Rep-VP123_P5-TATAless (ViralBrick-587KO-Z34C-Spacer)
creator: Freiburg Bioware 2010
created: 2010-08-27T11:00:00Z
modified: 2015-05-08T01:12:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 7049
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: p5TATAless
sequence annotation: TG-spacer
sequence annotation: GLS-spacer
sequence annotation: HSPG-KO
sequence annotation: downstream 587
sequence annotation: VP2
sequence annotation: Rep52
sequence annotation: Z34C
sequence annotation: upstream 587
sequence annotation: Rep78
sequence annotation: Rep68
sequence annotation: VP1
sequence annotation: VP3
sequence annotation: Rep40
sequence: https://synbiohub.org/public/igem/BBa_K404234_sequence/1

p5TATAless
type: SequenceAnnotation
persistent identity: annotation2098438
display id: annotation2098438
version: 1
top level: BBa_K404234
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K404234/annotation2098438/range2098438/1

https://synbiohub.org/public/igem/BBa_K404234/annotation2098438/range2098438/1
type: Range
persistent identity: range2098438
display id: range2098438
version: 1
direction: direction/forward
top level: BBa_K404234
start: 4339
end: 4502
orientation: inline

TG-spacer
type: SequenceAnnotation
persistent identity: annotation2098460
display id: annotation2098460
version: 1
top level: BBa_K404234
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404234/annotation2098460/range2098460/1

https://synbiohub.org/public/igem/BBa_K404234/annotation2098460/range2098460/1
type: Range
persistent identity: range2098460
display id: range2098460
version: 1
direction: direction/forward
top level: BBa_K404234
start: 3644
end: 3649
orientation: inline

GLS-spacer
type: SequenceAnnotation
persistent identity: annotation2098479
display id: annotation2098479
version: 1
top level: BBa_K404234
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404234/annotation2098479/range2098479/1

https://synbiohub.org/public/igem/BBa_K404234/annotation2098479/range2098479/1
type: Range
persistent identity: range2098479
display id: range2098479
version: 1
direction: direction/forward
top level: BBa_K404234
start: 3752
end: 3760
orientation: inline

HSPG-KO
type: SequenceAnnotation
persistent identity: annotation2101869
display id: annotation2101869
version: 1
top level: BBa_K404234
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K404234/annotation2101869/range2101869/1

https://synbiohub.org/public/igem/BBa_K404234/annotation2101869/range2101869/1
type: Range
persistent identity: range2101869
display id: range2101869
version: 1
direction: direction/forward
top level: BBa_K404234
start: 3753
end: 3755
orientation: inline

downstream 587
type: SequenceAnnotation
persistent identity: annotation2098500
display id: annotation2098500
version: 1
top level: BBa_K404234
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404234/annotation2098500/range2098500/1

https://synbiohub.org/public/igem/BBa_K404234/annotation2098500/range2098500/1
type: Range
persistent identity: range2098500
display id: range2098500
version: 1
direction: direction/forward
top level: BBa_K404234
start: 3761
end: 3776
orientation: inline

HSPG-KO
type: SequenceAnnotation
persistent identity: annotation2101870
display id: annotation2101870
version: 1
top level: BBa_K404234
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K404234/annotation2101870/range2101870/1

https://synbiohub.org/public/igem/BBa_K404234/annotation2101870/range2101870/1
type: Range
persistent identity: range2101870
display id: range2101870
version: 1
direction: direction/forward
top level: BBa_K404234
start: 3648
end: 3650
orientation: inline

VP2
type: SequenceAnnotation
persistent identity: annotation2098436
display id: annotation2098436
version: 1
top level: BBa_K404234
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404234/annotation2098436/range2098436/1

https://synbiohub.org/public/igem/BBa_K404234/annotation2098436/range2098436/1
type: Range
persistent identity: range2098436
display id: range2098436
version: 1
direction: direction/forward
top level: BBa_K404234
start: 2294
end: 4207
orientation: inline

Rep52
type: SequenceAnnotation
persistent identity: annotation2098432
display id: annotation2098432
version: 1
top level: BBa_K404234
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404234/annotation2098432/range2098432/1

https://synbiohub.org/public/igem/BBa_K404234/annotation2098432/range2098432/1
type: Range
persistent identity: range2098432
display id: range2098432
version: 1
direction: direction/forward
top level: BBa_K404234
start: 673
end: 1867
orientation: inline

Z34C
type: SequenceAnnotation
persistent identity: annotation2098439
display id: annotation2098439
version: 1
top level: BBa_K404234
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404234/annotation2098439/range2098439/1

https://synbiohub.org/public/igem/BBa_K404234/annotation2098439/range2098439/1
type: Range
persistent identity: range2098439
display id: range2098439
version: 1
direction: direction/forward
top level: BBa_K404234
start: 3650
end: 3751
orientation: inline

upstream 587
type: SequenceAnnotation
persistent identity: annotation2098435
display id: annotation2098435
version: 1
top level: BBa_K404234
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404234/annotation2098435/range2098435/1

https://synbiohub.org/public/igem/BBa_K404234/annotation2098435/range2098435/1
type: Range
persistent identity: range2098435
display id: range2098435
version: 1
direction: direction/forward
top level: BBa_K404234
start: 3612
end: 3643
orientation: inline

Rep78
type: SequenceAnnotation
persistent identity: annotation2098430
display id: annotation2098430
version: 1
top level: BBa_K404234
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404234/annotation2098430/range2098430/1

https://synbiohub.org/public/igem/BBa_K404234/annotation2098430/range2098430/1
type: Range
persistent identity: range2098430
display id: range2098430
version: 1
direction: direction/forward
top level: BBa_K404234
start: 2
end: 1867
orientation: inline

Rep68
type: SequenceAnnotation
persistent identity: annotation2098431
display id: annotation2098431
version: 1
top level: BBa_K404234
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404234/annotation2098431/range2098431/1

https://synbiohub.org/public/igem/BBa_K404234/annotation2098431/range2098431/1
type: Range
persistent identity: range2098431
display id: range2098431
version: 1
direction: direction/forward
top level: BBa_K404234
start: 2
end: 1933
orientation: inline

VP1
type: SequenceAnnotation
persistent identity: annotation2098434
display id: annotation2098434
version: 1
top level: BBa_K404234
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404234/annotation2098434/range2098434/1

https://synbiohub.org/public/igem/BBa_K404234/annotation2098434/range2098434/1
type: Range
persistent identity: range2098434
display id: range2098434
version: 1
direction: direction/forward
top level: BBa_K404234
start: 1883
end: 4207
orientation: inline

VP3
type: SequenceAnnotation
persistent identity: annotation2098437
display id: annotation2098437
version: 1
top level: BBa_K404234
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404234/annotation2098437/range2098437/1

https://synbiohub.org/public/igem/BBa_K404234/annotation2098437/range2098437/1
type: Range
persistent identity: range2098437
display id: range2098437
version: 1
direction: direction/forward
top level: BBa_K404234
start: 2489
end: 4207
orientation: inline

Rep40
type: SequenceAnnotation
persistent identity: annotation2098433
display id: annotation2098433
version: 1
top level: BBa_K404234
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404234/annotation2098433/range2098433/1

https://synbiohub.org/public/igem/BBa_K404234/annotation2098433/range2098433/1
type: Range
persistent identity: range2098433
display id: range2098433
version: 1
direction: direction/forward
top level: BBa_K404234
start: 673
end: 1933
orientation: inline

BBa_M1437
type: ComponentDefinition
persistent identity: BBa M1437
display id: BBa_M1437
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Enteropeptidase from Homo sapiens optimized for E. coli
creator: Trevor Russell Ballard
created: 2013-04-24T11:00:00Z
modified: 2015-05-08T01:13:56Z
discontinued: false
dominant: false
group u list: _768_
m user id: 0
owner id: 17280
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Enteropeptidase is an enzyme secreted in the small intestine necessary for digestion in humans. It is necessary for trypsin activation and acts as a serine protease.
mutable notes: Optimized for use in E. coli.
mutable provenance: http://www.uniprot.org/uniprot/P98073
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_M1437_sequence/1

BBa_K404211
type: ComponentDefinition
persistent identity: BBa K404211
display id: BBa_K404211
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: ViralBrick-587KO-His-Tag
creator: Freiburg Bioware 2010
created: 2010-08-27T11:00:00Z
modified: 2015-05-08T01:12:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 7049
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: downstream 587
sequence annotation: downstream 453
sequence annotation: R=>A
sequence annotation: upstream 587
sequence annotation: TG Spacer
sequence annotation: His-Tag
sequence annotation: upstream 453
sequence annotation: GLS Spacer
sequence: https://synbiohub.org/public/igem/BBa_K404211_sequence/1

downstream 587
type: SequenceAnnotation
persistent identity: annotation2079138
display id: annotation2079138
version: 1
top level: BBa_K404211
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404211/annotation2079138/range2079138/1

https://synbiohub.org/public/igem/BBa_K404211/annotation2079138/range2079138/1
type: Range
persistent identity: range2079138
display id: range2079138
version: 1
direction: direction/forward
top level: BBa_K404211
start: 76
end: 96
orientation: inline

downstream 453
type: SequenceAnnotation
persistent identity: annotation2079139
display id: annotation2079139
version: 1
top level: BBa_K404211
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404211/annotation2079139/range2079139/1

https://synbiohub.org/public/igem/BBa_K404211/annotation2079139/range2079139/1
type: Range
persistent identity: range2079139
display id: range2079139
version: 1
direction: direction/forward
top level: BBa_K404211
start: 97
end: 105
orientation: inline

R=>A
type: SequenceAnnotation
persistent identity: annotation2079133
display id: annotation2079133
version: 1
top level: BBa_K404211
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K404211/annotation2079133/range2079133/1

https://synbiohub.org/public/igem/BBa_K404211/annotation2079133/range2079133/1
type: Range
persistent identity: range2079133
display id: range2079133
version: 1
direction: direction/forward
top level: BBa_K404211
start: 34
end: 36
orientation: inline

upstream 587
type: SequenceAnnotation
persistent identity: annotation2079132
display id: annotation2079132
version: 1
top level: BBa_K404211
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404211/annotation2079132/range2079132/1

https://synbiohub.org/public/igem/BBa_K404211/annotation2079132/range2079132/1
type: Range
persistent identity: range2079132
display id: range2079132
version: 1
direction: direction/forward
top level: BBa_K404211
start: 10
end: 42
orientation: inline

TG Spacer
type: SequenceAnnotation
persistent identity: annotation2079134
display id: annotation2079134
version: 1
top level: BBa_K404211
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404211/annotation2079134/range2079134/1

https://synbiohub.org/public/igem/BBa_K404211/annotation2079134/range2079134/1
type: Range
persistent identity: range2079134
display id: range2079134
version: 1
direction: direction/forward
top level: BBa_K404211
start: 43
end: 48
orientation: inline

His-Tag
type: SequenceAnnotation
persistent identity: annotation2079135
display id: annotation2079135
version: 1
top level: BBa_K404211
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404211/annotation2079135/range2079135/1

https://synbiohub.org/public/igem/BBa_K404211/annotation2079135/range2079135/1
type: Range
persistent identity: range2079135
display id: range2079135
version: 1
direction: direction/forward
top level: BBa_K404211
start: 49
end: 66
orientation: inline

R=>A
type: SequenceAnnotation
persistent identity: annotation2079137
display id: annotation2079137
version: 1
top level: BBa_K404211
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K404211/annotation2079137/range2079137/1

https://synbiohub.org/public/igem/BBa_K404211/annotation2079137/range2079137/1
type: Range
persistent identity: range2079137
display id: range2079137
version: 1
direction: direction/forward
top level: BBa_K404211
start: 76
end: 78
orientation: inline

upstream 453
type: SequenceAnnotation
persistent identity: annotation2079131
display id: annotation2079131
version: 1
top level: BBa_K404211
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404211/annotation2079131/range2079131/1

https://synbiohub.org/public/igem/BBa_K404211/annotation2079131/range2079131/1
type: Range
persistent identity: range2079131
display id: range2079131
version: 1
direction: direction/forward
top level: BBa_K404211
start: 1
end: 9
orientation: inline

GLS Spacer
type: SequenceAnnotation
persistent identity: annotation2079136
display id: annotation2079136
version: 1
top level: BBa_K404211
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404211/annotation2079136/range2079136/1

https://synbiohub.org/public/igem/BBa_K404211/annotation2079136/range2079136/1
type: Range
persistent identity: range2079136
display id: range2079136
version: 1
direction: direction/forward
top level: BBa_K404211
start: 67
end: 75
orientation: inline

BBa_K258010
type: ComponentDefinition
persistent identity: BBa K258010
display id: BBa_K258010
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Human Epidermal growth factor (hEGF)
creator: Cihan Tastan and Ozkan IS
created: 2009-10-09T11:00:00Z
modified: 2015-05-08T01:11:42Z
discontinued: false
dominant: false
experience: experience/None
group u list: _358_
m user id: 0
owner id: 4260
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: Epidermal growth factor (EGF) is a single chain, 53 aminoacid-residue polypeptide (molecular weight 6045) and three intramolecular disulfide bonds. EGF is a low-molecular-weight polypeptide first purified from the mouse submandibular gland, but since then found in many human tissues including submandibular gland, parotid gland. EGF results in cellular proliferation, differentiation, and survival. EGF stimulates the growth of skin and corneal epithelium in vivo and in organ cultures. Epidermal growth factor (EGF) stimulates the growth of keratinocytes in vivo, and therefore plays an important role in the process of wound healing that depends on mitosis and migration of keratinocytes. Rhinewald and Green showed, in vitro that in the presence of growth factors , higher percentage of cells leave the resting state, enter and remain in the mitotic cycle. Mitogenic effect of EGF requires continuous exposure of target cells to EGF for a minimum 6-12 hours. The stimulation of wound healing by EGF has been confirmed as growth of granulation tissue in sponge implants used as inductive matrices. They observed, in rats, dose-dependent effect of EGF on granulation tissue formation as increased amounts of nucleic acids, and accumulation of collagen and glycosaminoglycans.
mutable notes: EGF with Biobrick standart restriction sites
mutable provenance: EGF is synthesized in human and some mammalians. Our EGF was synthesized synthetically at GENEART
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K258010_sequence/1

BBa_K1119009
type: ComponentDefinition
persistent identity: BBa K1119009
display id: BBa_K1119009
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CMV promoter - MLS - GFP - hGH polyA tail
creator: Hyun Jung Lee
created: 2013-09-15T11:00:00Z
modified: 2015-05-08T01:09:13Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1430_
m user id: 0
owner id: 17064
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: Later
mutable notes: Later
mutable provenance: Later
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: CMV promoter
sequence annotation: BBa_K1119001
sequence annotation: BBa_K1119006
sequence annotation: BBa_K404108
sequence annotation: hGH polya
sequence annotation: BBa_K648013
sequence annotation: MLS
sequence annotation: GFP
sequence: https://synbiohub.org/public/igem/BBa_K1119009_sequence/1

CMV promoter
type: SequenceAnnotation
persistent identity: annotation2363283
display id: annotation2363283
version: 1
top level: BBa_K1119009
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1119009/annotation2363283/range2363283/1

https://synbiohub.org/public/igem/BBa_K1119009/annotation2363283/range2363283/1
type: Range
persistent identity: range2363283
display id: range2363283
version: 1
direction: direction/forward
top level: BBa_K1119009
start: 1
end: 618
orientation: inline

BBa_K1119001
type: SequenceAnnotation
persistent identity: annotation2363264
display id: annotation2363264
version: 1
top level: BBa_K1119009
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1119009/annotation2363264/range2363264/1

https://synbiohub.org/public/igem/BBa_K1119009/annotation2363264/range2363264/1
type: Range
persistent identity: range2363264
display id: range2363264
version: 1
direction: direction/forward
top level: BBa_K1119009
start: 634
end: 720
orientation: inline

BBa_K1119006
type: SequenceAnnotation
persistent identity: annotation2363263
display id: annotation2363263
version: 1
top level: BBa_K1119009
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1119009/annotation2363263/range2363263/1

https://synbiohub.org/public/igem/BBa_K1119009/annotation2363263/range2363263/1
type: Range
persistent identity: range2363263
display id: range2363263
version: 1
direction: direction/forward
top level: BBa_K1119009
start: 1
end: 618
orientation: inline

BBa_K404108
type: SequenceAnnotation
persistent identity: annotation2363266
display id: annotation2363266
version: 1
top level: BBa_K1119009
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1119009/annotation2363266/range2363266/1

https://synbiohub.org/public/igem/BBa_K1119009/annotation2363266/range2363266/1
type: Range
persistent identity: range2363266
display id: range2363266
version: 1
direction: direction/forward
top level: BBa_K1119009
start: 1479
end: 1959
orientation: inline

hGH polya
type: SequenceAnnotation
persistent identity: annotation2363286
display id: annotation2363286
version: 1
top level: BBa_K1119009
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_K1119009/annotation2363286/range2363286/1

https://synbiohub.org/public/igem/BBa_K1119009/annotation2363286/range2363286/1
type: Range
persistent identity: range2363286
display id: range2363286
version: 1
direction: direction/forward
top level: BBa_K1119009
start: 1479
end: 1959
orientation: inline

BBa_K648013
type: SequenceAnnotation
persistent identity: annotation2363265
display id: annotation2363265
version: 1
top level: BBa_K1119009
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1119009/annotation2363265/range2363265/1

https://synbiohub.org/public/igem/BBa_K1119009/annotation2363265/range2363265/1
type: Range
persistent identity: range2363265
display id: range2363265
version: 1
direction: direction/forward
top level: BBa_K1119009
start: 727
end: 1461
orientation: inline

MLS
type: SequenceAnnotation
persistent identity: annotation2363284
display id: annotation2363284
version: 1
top level: BBa_K1119009
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1119009/annotation2363284/range2363284/1

https://synbiohub.org/public/igem/BBa_K1119009/annotation2363284/range2363284/1
type: Range
persistent identity: range2363284
display id: range2363284
version: 1
direction: direction/forward
top level: BBa_K1119009
start: 634
end: 720
orientation: inline

GFP
type: SequenceAnnotation
persistent identity: annotation2363285
display id: annotation2363285
version: 1
top level: BBa_K1119009
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1119009/annotation2363285/range2363285/1

https://synbiohub.org/public/igem/BBa_K1119009/annotation2363285/range2363285/1
type: Range
persistent identity: range2363285
display id: range2363285
version: 1
direction: direction/forward
top level: BBa_K1119009
start: 727
end: 1461
orientation: inline

BBa_K404117
type: ComponentDefinition
persistent identity: BBa K404117
display id: BBa_K404117
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: [AAV2]-left-ITR_pCMV_betaglobin
creator: Freiburg Bioware 2010
created: 2010-10-10T11:00:00Z
modified: 2015-05-08T01:12:23Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 6733
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: CMV promoter
sequence annotation: Beta-Globin-intron
sequence annotation: left ITR
sequence: https://synbiohub.org/public/igem/BBa_K404117_sequence/1

CMV promoter
type: SequenceAnnotation
persistent identity: annotation2093231
display id: annotation2093231
version: 1
top level: BBa_K404117
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K404117/annotation2093231/range2093231/1

https://synbiohub.org/public/igem/BBa_K404117/annotation2093231/range2093231/1
type: Range
persistent identity: range2093231
display id: range2093231
version: 1
direction: direction/forward
top level: BBa_K404117
start: 155
end: 809
orientation: inline

Beta-Globin-intron
type: SequenceAnnotation
persistent identity: annotation2093232
display id: annotation2093232
version: 1
top level: BBa_K404117
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404117/annotation2093232/range2093232/1

https://synbiohub.org/public/igem/BBa_K404117/annotation2093232/range2093232/1
type: Range
persistent identity: range2093232
display id: range2093232
version: 1
direction: direction/forward
top level: BBa_K404117
start: 816
end: 1310
orientation: inline

left ITR
type: SequenceAnnotation
persistent identity: annotation2093230
display id: annotation2093230
version: 1
top level: BBa_K404117
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404117/annotation2093230/range2093230/1

https://synbiohub.org/public/igem/BBa_K404117/annotation2093230/range2093230/1
type: Range
persistent identity: range2093230
display id: range2093230
version: 1
direction: direction/forward
top level: BBa_K404117
start: 1
end: 147
orientation: inline

BBa_K404002
type: ComponentDefinition
persistent identity: BBa K404002
display id: BBa_K404002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: [AAV2]-Rep-VP123_p5-TATAless
creator: Freiburg Bioware 2010
created: 2010-10-20T11:00:00Z
modified: 2015-05-08T01:12:23Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 6674
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: a
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: Rep78
sequence annotation: p5TATAless
sequence annotation: Rep52
sequence annotation: VP2
sequence annotation: Rep68
sequence annotation: VP1
sequence annotation: VP3
sequence annotation: AAV-2-cap (Stratagene)
sequence annotation: Rep40
sequence: https://synbiohub.org/public/igem/BBa_K404002_sequence/1

Rep78
type: SequenceAnnotation
persistent identity: annotation2097807
display id: annotation2097807
version: 1
top level: BBa_K404002
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404002/annotation2097807/range2097807/1

https://synbiohub.org/public/igem/BBa_K404002/annotation2097807/range2097807/1
type: Range
persistent identity: range2097807
display id: range2097807
version: 1
direction: direction/forward
top level: BBa_K404002
start: 1
end: 1865
orientation: inline

p5TATAless
type: SequenceAnnotation
persistent identity: annotation2097816
display id: annotation2097816
version: 1
top level: BBa_K404002
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K404002/annotation2097816/range2097816/1

https://synbiohub.org/public/igem/BBa_K404002/annotation2097816/range2097816/1
type: Range
persistent identity: range2097816
display id: range2097816
version: 1
direction: direction/forward
top level: BBa_K404002
start: 4220
end: 4383
orientation: inline

Rep52
type: SequenceAnnotation
persistent identity: annotation2097810
display id: annotation2097810
version: 1
top level: BBa_K404002
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404002/annotation2097810/range2097810/1

https://synbiohub.org/public/igem/BBa_K404002/annotation2097810/range2097810/1
type: Range
persistent identity: range2097810
display id: range2097810
version: 1
direction: direction/forward
top level: BBa_K404002
start: 671
end: 1865
orientation: inline

VP2
type: SequenceAnnotation
persistent identity: annotation2097812
display id: annotation2097812
version: 1
top level: BBa_K404002
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404002/annotation2097812/range2097812/1

https://synbiohub.org/public/igem/BBa_K404002/annotation2097812/range2097812/1
type: Range
persistent identity: range2097812
display id: range2097812
version: 1
direction: direction/forward
top level: BBa_K404002
start: 2292
end: 4088
orientation: inline

Rep68
type: SequenceAnnotation
persistent identity: annotation2097808
display id: annotation2097808
version: 1
top level: BBa_K404002
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404002/annotation2097808/range2097808/1

https://synbiohub.org/public/igem/BBa_K404002/annotation2097808/range2097808/1
type: Range
persistent identity: range2097808
display id: range2097808
version: 1
direction: direction/forward
top level: BBa_K404002
start: 1
end: 1931
orientation: inline

VP1
type: SequenceAnnotation
persistent identity: annotation2097811
display id: annotation2097811
version: 1
top level: BBa_K404002
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404002/annotation2097811/range2097811/1

https://synbiohub.org/public/igem/BBa_K404002/annotation2097811/range2097811/1
type: Range
persistent identity: range2097811
display id: range2097811
version: 1
direction: direction/forward
top level: BBa_K404002
start: 1881
end: 4088
orientation: inline

VP3
type: SequenceAnnotation
persistent identity: annotation2097813
display id: annotation2097813
version: 1
top level: BBa_K404002
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404002/annotation2097813/range2097813/1

https://synbiohub.org/public/igem/BBa_K404002/annotation2097813/range2097813/1
type: Range
persistent identity: range2097813
display id: range2097813
version: 1
direction: direction/forward
top level: BBa_K404002
start: 2487
end: 4088
orientation: inline

AAV-2-cap (Stratagene)
type: SequenceAnnotation
persistent identity: annotation2097814
display id: annotation2097814
version: 1
top level: BBa_K404002
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404002/annotation2097814/range2097814/1

https://synbiohub.org/public/igem/BBa_K404002/annotation2097814/range2097814/1
type: Range
persistent identity: range2097814
display id: range2097814
version: 1
direction: direction/forward
top level: BBa_K404002
start: 1881
end: 4212
orientation: inline

Rep40
type: SequenceAnnotation
persistent identity: annotation2097809
display id: annotation2097809
version: 1
top level: BBa_K404002
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404002/annotation2097809/range2097809/1

https://synbiohub.org/public/igem/BBa_K404002/annotation2097809/range2097809/1
type: Range
persistent identity: range2097809
display id: range2097809
version: 1
direction: direction/forward
top level: BBa_K404002
start: 671
end: 1931
orientation: inline

BBa_K404242
type: ComponentDefinition
persistent identity: BBa K404242
display id: BBa_K404242
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pCMV_[AAV2]VP123 (ViralBrick-453-His-Tag)
creator: Freiburg Bioware 2010
created: 2010-09-19T11:00:00Z
modified: 2015-05-08T01:12:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 7049
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: upstrem 453
sequence annotation: downstream 453
sequence annotation: CMV
sequence annotation: AAP
sequence annotation: His Tag
sequence annotation: VP2
sequence annotation: AAV-2-cap (Stratagene)
sequence annotation: VP1
sequence annotation: VP3
sequence: https://synbiohub.org/public/igem/BBa_K404242_sequence/1

upstrem 453
type: SequenceAnnotation
persistent identity: annotation2094175
display id: annotation2094175
version: 1
top level: BBa_K404242
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404242/annotation2094175/range2094175/1

https://synbiohub.org/public/igem/BBa_K404242/annotation2094175/range2094175/1
type: Range
persistent identity: range2094175
display id: range2094175
version: 1
direction: direction/forward
top level: BBa_K404242
start: 1989
end: 2026
orientation: inline

downstream 453
type: SequenceAnnotation
persistent identity: annotation2094177
display id: annotation2094177
version: 1
top level: BBa_K404242
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404242/annotation2094177/range2094177/1

https://synbiohub.org/public/igem/BBa_K404242/annotation2094177/range2094177/1
type: Range
persistent identity: range2094177
display id: range2094177
version: 1
direction: direction/forward
top level: BBa_K404242
start: 2051
end: 2064
orientation: inline

CMV
type: SequenceAnnotation
persistent identity: annotation2094170
display id: annotation2094170
version: 1
top level: BBa_K404242
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K404242/annotation2094170/range2094170/1

https://synbiohub.org/public/igem/BBa_K404242/annotation2094170/range2094170/1
type: Range
persistent identity: range2094170
display id: range2094170
version: 1
direction: direction/forward
top level: BBa_K404242
start: 2
end: 655
orientation: inline

AAP
type: SequenceAnnotation
persistent identity: annotation2094205
display id: annotation2094205
version: 1
top level: BBa_K404242
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404242/annotation2094205/range2094205/1

https://synbiohub.org/public/igem/BBa_K404242/annotation2094205/range2094205/1
type: Range
persistent identity: range2094205
display id: range2094205
version: 1
direction: direction/forward
top level: BBa_K404242
start: 1194
end: 1808
orientation: inline

His Tag
type: SequenceAnnotation
persistent identity: annotation2094176
display id: annotation2094176
version: 1
top level: BBa_K404242
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404242/annotation2094176/range2094176/1

https://synbiohub.org/public/igem/BBa_K404242/annotation2094176/range2094176/1
type: Range
persistent identity: range2094176
display id: range2094176
version: 1
direction: direction/forward
top level: BBa_K404242
start: 2030
end: 2047
orientation: inline

VP2
type: SequenceAnnotation
persistent identity: annotation2094172
display id: annotation2094172
version: 1
top level: BBa_K404242
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404242/annotation2094172/range2094172/1

https://synbiohub.org/public/igem/BBa_K404242/annotation2094172/range2094172/1
type: Range
persistent identity: range2094172
display id: range2094172
version: 1
direction: direction/forward
top level: BBa_K404242
start: 1079
end: 2899
orientation: inline

AAV-2-cap (Stratagene)
type: SequenceAnnotation
persistent identity: annotation2094174
display id: annotation2094174
version: 1
top level: BBa_K404242
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404242/annotation2094174/range2094174/1

https://synbiohub.org/public/igem/BBa_K404242/annotation2094174/range2094174/1
type: Range
persistent identity: range2094174
display id: range2094174
version: 1
direction: direction/forward
top level: BBa_K404242
start: 671
end: 3022
orientation: inline

VP1
type: SequenceAnnotation
persistent identity: annotation2094171
display id: annotation2094171
version: 1
top level: BBa_K404242
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404242/annotation2094171/range2094171/1

https://synbiohub.org/public/igem/BBa_K404242/annotation2094171/range2094171/1
type: Range
persistent identity: range2094171
display id: range2094171
version: 1
direction: direction/forward
top level: BBa_K404242
start: 671
end: 2899
orientation: inline

VP3
type: SequenceAnnotation
persistent identity: annotation2094173
display id: annotation2094173
version: 1
top level: BBa_K404242
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404242/annotation2094173/range2094173/1

https://synbiohub.org/public/igem/BBa_K404242/annotation2094173/range2094173/1
type: Range
persistent identity: range2094173
display id: range2094173
version: 1
direction: direction/forward
top level: BBa_K404242
start: 1274
end: 2899
orientation: inline

BGH pA
type: ComponentDefinition
persistent identity: BBa K678019
display id: BBa_K678019
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: BGH poly A, mammalian terminator
creator: DTU-Denmark-2
created: 2011-09-03T11:00:00Z
modified: 2015-05-08T01:13:04Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _882_
m user id: 0
owner id: 8341
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: BGH poly A, terminator for use in mammalian cells
mutable notes: Compatible with the Plug'n'Play assembly standard
mutable provenance: From plasmid pcDNA3.1 from Invitrogen
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BGH
sequence annotation: Linker 4
sequence: https://synbiohub.org/public/igem/BBa_K678019_sequence/1

BGH
type: SequenceAnnotation
persistent identity: annotation2127353
display id: annotation2127353
version: 1
top level: BGH pA
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_K678019/annotation2127353/range2127353/1

https://synbiohub.org/public/igem/BBa_K678019/annotation2127353/range2127353/1
type: Range
persistent identity: range2127353
display id: range2127353
version: 1
direction: direction/forward
top level: BGH pA
start: 9
end: 233
orientation: inline

Linker 4
type: SequenceAnnotation
persistent identity: annotation2127352
display id: annotation2127352
version: 1
top level: BGH pA
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K678019/annotation2127352/range2127352/1

https://synbiohub.org/public/igem/BBa_K678019/annotation2127352/range2127352/1
type: Range
persistent identity: range2127352
display id: range2127352
version: 1
direction: direction/forward
top level: BGH pA
start: 1
end: 8
orientation: inline

BBa_K404005
type: ComponentDefinition
persistent identity: BBa K404005
display id: BBa_K404005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: [AAV2]-Rep-VP23(ViralBrick-587KO-empty)_p5-TATAless
creator: Freiburg Bioware 2010
created: 2010-10-20T11:00:00Z
modified: 2015-05-08T01:12:23Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 6674
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: Rep40
sequence annotation: p5TATAless
sequence annotation: VP2
sequence annotation: VP3
sequence annotation: Rep78
sequence annotation: HSPG-KO
sequence annotation: Rep68
sequence annotation: Rep52
sequence: https://synbiohub.org/public/igem/BBa_K404005_sequence/1

Rep40
type: SequenceAnnotation
persistent identity: annotation2097886
display id: annotation2097886
version: 1
top level: BBa_K404005
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404005/annotation2097886/range2097886/1

https://synbiohub.org/public/igem/BBa_K404005/annotation2097886/range2097886/1
type: Range
persistent identity: range2097886
display id: range2097886
version: 1
direction: direction/forward
top level: BBa_K404005
start: 673
end: 1933
orientation: inline

p5TATAless
type: SequenceAnnotation
persistent identity: annotation2097890
display id: annotation2097890
version: 1
top level: BBa_K404005
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K404005/annotation2097890/range2097890/1

https://synbiohub.org/public/igem/BBa_K404005/annotation2097890/range2097890/1
type: Range
persistent identity: range2097890
display id: range2097890
version: 1
direction: direction/forward
top level: BBa_K404005
start: 4238
end: 4401
orientation: inline

VP2
type: SequenceAnnotation
persistent identity: annotation2097888
display id: annotation2097888
version: 1
top level: BBa_K404005
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404005/annotation2097888/range2097888/1

https://synbiohub.org/public/igem/BBa_K404005/annotation2097888/range2097888/1
type: Range
persistent identity: range2097888
display id: range2097888
version: 1
direction: direction/forward
top level: BBa_K404005
start: 2294
end: 4090
orientation: inline

VP3
type: SequenceAnnotation
persistent identity: annotation2097889
display id: annotation2097889
version: 1
top level: BBa_K404005
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404005/annotation2097889/range2097889/1

https://synbiohub.org/public/igem/BBa_K404005/annotation2097889/range2097889/1
type: Range
persistent identity: range2097889
display id: range2097889
version: 1
direction: direction/forward
top level: BBa_K404005
start: 2489
end: 4090
orientation: inline

Rep78
type: SequenceAnnotation
persistent identity: annotation2097885
display id: annotation2097885
version: 1
top level: BBa_K404005
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404005/annotation2097885/range2097885/1

https://synbiohub.org/public/igem/BBa_K404005/annotation2097885/range2097885/1
type: Range
persistent identity: range2097885
display id: range2097885
version: 1
direction: direction/forward
top level: BBa_K404005
start: 1
end: 1933
orientation: inline

HSPG-KO
type: SequenceAnnotation
persistent identity: annotation2101714
display id: annotation2101714
version: 1
top level: BBa_K404005
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K404005/annotation2101714/range2101714/1

https://synbiohub.org/public/igem/BBa_K404005/annotation2101714/range2101714/1
type: Range
persistent identity: range2101714
display id: range2101714
version: 1
direction: direction/forward
top level: BBa_K404005
start: 3635
end: 3637
orientation: inline

Rep68
type: SequenceAnnotation
persistent identity: annotation2097884
display id: annotation2097884
version: 1
top level: BBa_K404005
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404005/annotation2097884/range2097884/1

https://synbiohub.org/public/igem/BBa_K404005/annotation2097884/range2097884/1
type: Range
persistent identity: range2097884
display id: range2097884
version: 1
direction: direction/forward
top level: BBa_K404005
start: 1
end: 1867
orientation: inline

HSPG-KO
type: SequenceAnnotation
persistent identity: annotation2101713
display id: annotation2101713
version: 1
top level: BBa_K404005
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K404005/annotation2101713/range2101713/1

https://synbiohub.org/public/igem/BBa_K404005/annotation2101713/range2101713/1
type: Range
persistent identity: range2101713
display id: range2101713
version: 1
direction: direction/forward
top level: BBa_K404005
start: 3644
end: 3646
orientation: inline

Rep52
type: SequenceAnnotation
persistent identity: annotation2097887
display id: annotation2097887
version: 1
top level: BBa_K404005
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404005/annotation2097887/range2097887/1

https://synbiohub.org/public/igem/BBa_K404005/annotation2097887/range2097887/1
type: Range
persistent identity: range2097887
display id: range2097887
version: 1
direction: direction/forward
top level: BBa_K404005
start: 673
end: 1867
orientation: inline

BBa_K404121
type: ComponentDefinition
persistent identity: BBa K404121
display id: BBa_K404121
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: [AAV2]-left-ITR_pCMV_betaglobin_mCherry_hGH_[AAV2]-right-ITR
creator: Freiburg Bioware 2010
created: 2010-10-10T11:00:00Z
modified: 2015-05-08T01:12:23Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 6733
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: rrnB_T1
sequence annotation: mCherry
sequence annotation: right ITR
sequence annotation: CMV promoter
sequence annotation: Beta-Globin-intron
sequence annotation: left ITR
sequence annotation: stem loop early T7
sequence annotation: hGH
sequence: https://synbiohub.org/public/igem/BBa_K404121_sequence/1

rrnB_T1
type: SequenceAnnotation
persistent identity: annotation2093263
display id: annotation2093263
version: 1
top level: BBa_K404121
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404121/annotation2093263/range2093263/1

https://synbiohub.org/public/igem/BBa_K404121/annotation2093263/range2093263/1
type: Range
persistent identity: range2093263
display id: range2093263
version: 1
direction: direction/forward
top level: BBa_K404121
start: 2069
end: 2112
orientation: inline

mCherry
type: SequenceAnnotation
persistent identity: annotation2093262
display id: annotation2093262
version: 1
top level: BBa_K404121
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404121/annotation2093262/range2093262/1

https://synbiohub.org/public/igem/BBa_K404121/annotation2093262/range2093262/1
type: Range
persistent identity: range2093262
display id: range2093262
version: 1
direction: direction/forward
top level: BBa_K404121
start: 1336
end: 2046
orientation: inline

right ITR
type: SequenceAnnotation
persistent identity: annotation2093265
display id: annotation2093265
version: 1
top level: BBa_K404121
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404121/annotation2093265/range2093265/1

https://synbiohub.org/public/igem/BBa_K404121/annotation2093265/range2093265/1
type: Range
persistent identity: range2093265
display id: range2093265
version: 1
direction: direction/forward
top level: BBa_K404121
start: 2688
end: 2826
orientation: inline

CMV promoter
type: SequenceAnnotation
persistent identity: annotation2093260
display id: annotation2093260
version: 1
top level: BBa_K404121
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K404121/annotation2093260/range2093260/1

https://synbiohub.org/public/igem/BBa_K404121/annotation2093260/range2093260/1
type: Range
persistent identity: range2093260
display id: range2093260
version: 1
direction: direction/forward
top level: BBa_K404121
start: 155
end: 809
orientation: inline

Beta-Globin-intron
type: SequenceAnnotation
persistent identity: annotation2093261
display id: annotation2093261
version: 1
top level: BBa_K404121
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404121/annotation2093261/range2093261/1

https://synbiohub.org/public/igem/BBa_K404121/annotation2093261/range2093261/1
type: Range
persistent identity: range2093261
display id: range2093261
version: 1
direction: direction/forward
top level: BBa_K404121
start: 816
end: 1310
orientation: inline

left ITR
type: SequenceAnnotation
persistent identity: annotation2093259
display id: annotation2093259
version: 1
top level: BBa_K404121
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404121/annotation2093259/range2093259/1

https://synbiohub.org/public/igem/BBa_K404121/annotation2093259/range2093259/1
type: Range
persistent identity: range2093259
display id: range2093259
version: 1
direction: direction/forward
top level: BBa_K404121
start: 1
end: 147
orientation: inline

stem loop early T7
type: SequenceAnnotation
persistent identity: annotation2093264
display id: annotation2093264
version: 1
top level: BBa_K404121
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404121/annotation2093264/range2093264/1

https://synbiohub.org/public/igem/BBa_K404121/annotation2093264/range2093264/1
type: Range
persistent identity: range2093264
display id: range2093264
version: 1
direction: direction/forward
top level: BBa_K404121
start: 2153
end: 2172
orientation: inline

hGH
type: SequenceAnnotation
persistent identity: annotation2093266
display id: annotation2093266
version: 1
top level: BBa_K404121
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404121/annotation2093266/range2093266/1

https://synbiohub.org/public/igem/BBa_K404121/annotation2093266/range2093266/1
type: Range
persistent identity: range2093266
display id: range2093266
version: 1
direction: direction/forward
top level: BBa_K404121
start: 2198
end: 2664
orientation: inline

BBa_K404001
type: ComponentDefinition
persistent identity: BBa K404001
display id: BBa_K404001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: [AAV2]-Rep-VP123
creator: Team Freiburg 2010
created: 2010-08-22T11:00:00Z
modified: 2015-05-08T01:12:23Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 7049
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Test long
mutable notes: Test design considerations
mutable provenance: Test source
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: Rep68
sequence annotation: Rep40
sequence annotation: Rep52
sequence annotation: VP2
sequence annotation: VP1
sequence annotation: Rep78
sequence annotation: AAV-2-cap (Stratagene)
sequence annotation: VP3
sequence: https://synbiohub.org/public/igem/BBa_K404001_sequence/1

Rep68
type: SequenceAnnotation
persistent identity: annotation2097892
display id: annotation2097892
version: 1
top level: BBa_K404001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404001/annotation2097892/range2097892/1

https://synbiohub.org/public/igem/BBa_K404001/annotation2097892/range2097892/1
type: Range
persistent identity: range2097892
display id: range2097892
version: 1
direction: direction/forward
top level: BBa_K404001
start: 2
end: 1933
orientation: inline

Rep40
type: SequenceAnnotation
persistent identity: annotation2097894
display id: annotation2097894
version: 1
top level: BBa_K404001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404001/annotation2097894/range2097894/1

https://synbiohub.org/public/igem/BBa_K404001/annotation2097894/range2097894/1
type: Range
persistent identity: range2097894
display id: range2097894
version: 1
direction: direction/forward
top level: BBa_K404001
start: 673
end: 1933
orientation: inline

Rep52
type: SequenceAnnotation
persistent identity: annotation2097893
display id: annotation2097893
version: 1
top level: BBa_K404001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404001/annotation2097893/range2097893/1

https://synbiohub.org/public/igem/BBa_K404001/annotation2097893/range2097893/1
type: Range
persistent identity: range2097893
display id: range2097893
version: 1
direction: direction/forward
top level: BBa_K404001
start: 673
end: 1867
orientation: inline

VP2
type: SequenceAnnotation
persistent identity: annotation2097896
display id: annotation2097896
version: 1
top level: BBa_K404001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404001/annotation2097896/range2097896/1

https://synbiohub.org/public/igem/BBa_K404001/annotation2097896/range2097896/1
type: Range
persistent identity: range2097896
display id: range2097896
version: 1
direction: direction/forward
top level: BBa_K404001
start: 2294
end: 4090
orientation: inline

VP1
type: SequenceAnnotation
persistent identity: annotation2097895
display id: annotation2097895
version: 1
top level: BBa_K404001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404001/annotation2097895/range2097895/1

https://synbiohub.org/public/igem/BBa_K404001/annotation2097895/range2097895/1
type: Range
persistent identity: range2097895
display id: range2097895
version: 1
direction: direction/forward
top level: BBa_K404001
start: 1883
end: 4090
orientation: inline

Rep78
type: SequenceAnnotation
persistent identity: annotation2097891
display id: annotation2097891
version: 1
top level: BBa_K404001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404001/annotation2097891/range2097891/1

https://synbiohub.org/public/igem/BBa_K404001/annotation2097891/range2097891/1
type: Range
persistent identity: range2097891
display id: range2097891
version: 1
direction: direction/forward
top level: BBa_K404001
start: 2
end: 1867
orientation: inline

AAV-2-cap (Stratagene)
type: SequenceAnnotation
persistent identity: annotation2097900
display id: annotation2097900
version: 1
top level: BBa_K404001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404001/annotation2097900/range2097900/1

https://synbiohub.org/public/igem/BBa_K404001/annotation2097900/range2097900/1
type: Range
persistent identity: range2097900
display id: range2097900
version: 1
direction: direction/forward
top level: BBa_K404001
start: 1883
end: 4213
orientation: inline

VP3
type: SequenceAnnotation
persistent identity: annotation2097899
display id: annotation2097899
version: 1
top level: BBa_K404001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404001/annotation2097899/range2097899/1

https://synbiohub.org/public/igem/BBa_K404001/annotation2097899/range2097899/1
type: Range
persistent identity: range2097899
display id: range2097899
version: 1
direction: direction/forward
top level: BBa_K404001
start: 2489
end: 4090
orientation: inline

BBa_I712024
type: ComponentDefinition
persistent identity: BBa I712024
display id: BBa_I712024
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CD4
creator: Katja Kolar
created: 2007-10-22T11:00:00Z
modified: 2015-08-31T04:07:46Z
discontinued: false
dominant: false
group u list: _130_
m user id: 0
owner id: 1883
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Sequence for chemokine receptor CD4, including signal peptide.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_I712024_sequence/1

eGFP-PTS1
type: ComponentDefinition
persistent identity: BBa K678002
display id: BBa_K678002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: eGFP-PTS1 for mammalian cells
creator: DTU-Denmark-2
created: 2011-09-01T11:00:00Z
modified: 2015-05-08T01:13:04Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _882_
m user id: 0
owner id: 8341
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: Vector expressing eGFP from the CMV promoter with the BGH polyA tail and with a hygromycin resistance cassette
mutable notes: Compatible with the Plug'n'Play with DNA assembly system.
mutable provenance: Source to be announced
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
sequence annotation: PTS1
sequence annotation: Kozak
sequence annotation: Linker 6
sequence annotation: eGFP
sequence annotation: Linker 5
sequence annotation: CMV
sequence annotation: Linker 2
sequence annotation: Linker 4
sequence annotation: Linker 1
sequence annotation: Hygromycin cassette
sequence annotation: BGH pA
sequence: https://synbiohub.org/public/igem/BBa_K678002_sequence/1

PTS1
type: SequenceAnnotation
persistent identity: annotation2126936
display id: annotation2126936
version: 1
top level: eGFP-PTS1
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K678002/annotation2126936/range2126936/1

https://synbiohub.org/public/igem/BBa_K678002/annotation2126936/range2126936/1
type: Range
persistent identity: range2126936
display id: range2126936
version: 1
direction: direction/forward
top level: eGFP-PTS1
start: 1395
end: 1406
orientation: inline

Kozak
type: SequenceAnnotation
persistent identity: annotation2126935
display id: annotation2126935
version: 1
top level: eGFP-PTS1
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K678002/annotation2126935/range2126935/1

https://synbiohub.org/public/igem/BBa_K678002/annotation2126935/range2126935/1
type: Range
persistent identity: range2126935
display id: range2126935
version: 1
direction: direction/forward
top level: eGFP-PTS1
start: 672
end: 682
orientation: inline

Linker 6
type: SequenceAnnotation
persistent identity: annotation2126941
display id: annotation2126941
version: 1
top level: eGFP-PTS1
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K678002/annotation2126941/range2126941/1

https://synbiohub.org/public/igem/BBa_K678002/annotation2126941/range2126941/1
type: Range
persistent identity: range2126941
display id: range2126941
version: 1
direction: direction/forward
top level: eGFP-PTS1
start: 3399
end: 3406
orientation: inline

eGFP
type: SequenceAnnotation
persistent identity: annotation2126934
display id: annotation2126934
version: 1
top level: eGFP-PTS1
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K678002/annotation2126934/range2126934/1

https://synbiohub.org/public/igem/BBa_K678002/annotation2126934/range2126934/1
type: Range
persistent identity: range2126934
display id: range2126934
version: 1
direction: direction/forward
top level: eGFP-PTS1
start: 679
end: 1394
orientation: inline

Linker 5
type: SequenceAnnotation
persistent identity: annotation2126939
display id: annotation2126939
version: 1
top level: eGFP-PTS1
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K678002/annotation2126939/range2126939/1

https://synbiohub.org/public/igem/BBa_K678002/annotation2126939/range2126939/1
type: Range
persistent identity: range2126939
display id: range2126939
version: 1
direction: direction/forward
top level: eGFP-PTS1
start: 1640
end: 1647
orientation: inline

CMV
type: SequenceAnnotation
persistent identity: annotation2126913
display id: annotation2126913
version: 1
top level: eGFP-PTS1
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K678002/annotation2126913/range2126913/1

https://synbiohub.org/public/igem/BBa_K678002/annotation2126913/range2126913/1
type: Range
persistent identity: range2126913
display id: range2126913
version: 1
direction: direction/forward
top level: eGFP-PTS1
start: 9
end: 662
orientation: inline

Linker 2
type: SequenceAnnotation
persistent identity: annotation2126914
display id: annotation2126914
version: 1
top level: eGFP-PTS1
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K678002/annotation2126914/range2126914/1

https://synbiohub.org/public/igem/BBa_K678002/annotation2126914/range2126914/1
type: Range
persistent identity: range2126914
display id: range2126914
version: 1
direction: direction/forward
top level: eGFP-PTS1
start: 663
end: 671
orientation: inline

Linker 4
type: SequenceAnnotation
persistent identity: annotation2126937
display id: annotation2126937
version: 1
top level: eGFP-PTS1
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K678002/annotation2126937/range2126937/1

https://synbiohub.org/public/igem/BBa_K678002/annotation2126937/range2126937/1
type: Range
persistent identity: range2126937
display id: range2126937
version: 1
direction: direction/forward
top level: eGFP-PTS1
start: 1407
end: 1414
orientation: inline

Linker 1
type: SequenceAnnotation
persistent identity: annotation2126912
display id: annotation2126912
version: 1
top level: eGFP-PTS1
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K678002/annotation2126912/range2126912/1

https://synbiohub.org/public/igem/BBa_K678002/annotation2126912/range2126912/1
type: Range
persistent identity: range2126912
display id: range2126912
version: 1
direction: direction/forward
top level: eGFP-PTS1
start: 1
end: 8
orientation: inline

Hygromycin cassette
type: SequenceAnnotation
persistent identity: annotation2126940
display id: annotation2126940
version: 1
top level: eGFP-PTS1
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K678002/annotation2126940/range2126940/1

https://synbiohub.org/public/igem/BBa_K678002/annotation2126940/range2126940/1
type: Range
persistent identity: range2126940
display id: range2126940
version: 1
direction: direction/forward
top level: eGFP-PTS1
start: 1648
end: 3398
orientation: inline

BGH pA
type: SequenceAnnotation
persistent identity: annotation2126938
display id: annotation2126938
version: 1
top level: eGFP-PTS1
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_K678002/annotation2126938/range2126938/1

https://synbiohub.org/public/igem/BBa_K678002/annotation2126938/range2126938/1
type: Range
persistent identity: range2126938
display id: range2126938
version: 1
direction: direction/forward
top level: eGFP-PTS1
start: 1415
end: 1639
orientation: inline

BBa_K404154
type: ComponentDefinition
persistent identity: BBa K404154
display id: BBa_K404154
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pCMV_Z-EGFR-1907_[AAV2]-VP23 (ViralBrick-587KO-Empty)
creator: Freiburg Bioware 2010
created: 2010-10-11T11:00:00Z
modified: 2015-05-08T01:12:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 7049
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: VP3
sequence annotation: AAV-2-cap (Stratagene)
sequence annotation: VP2
sequence annotation: CMV
sequence annotation: ZEGFR_1907
sequence annotation: HSPG-KO
sequence: https://synbiohub.org/public/igem/BBa_K404154_sequence/1

VP3
type: SequenceAnnotation
persistent identity: annotation2097912
display id: annotation2097912
version: 1
top level: BBa_K404154
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404154/annotation2097912/range2097912/1

https://synbiohub.org/public/igem/BBa_K404154/annotation2097912/range2097912/1
type: Range
persistent identity: range2097912
display id: range2097912
version: 1
direction: direction/forward
top level: BBa_K404154
start: 1043
end: 2644
orientation: inline

AAV-2-cap (Stratagene)
type: SequenceAnnotation
persistent identity: annotation2097913
display id: annotation2097913
version: 1
top level: BBa_K404154
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404154/annotation2097913/range2097913/1

https://synbiohub.org/public/igem/BBa_K404154/annotation2097913/range2097913/1
type: Range
persistent identity: range2097913
display id: range2097913
version: 1
direction: direction/forward
top level: BBa_K404154
start: 851
end: 2767
orientation: inline

VP2
type: SequenceAnnotation
persistent identity: annotation2097910
display id: annotation2097910
version: 1
top level: BBa_K404154
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404154/annotation2097910/range2097910/1

https://synbiohub.org/public/igem/BBa_K404154/annotation2097910/range2097910/1
type: Range
persistent identity: range2097910
display id: range2097910
version: 1
direction: direction/forward
top level: BBa_K404154
start: 851
end: 2644
orientation: inline

CMV
type: SequenceAnnotation
persistent identity: annotation2097908
display id: annotation2097908
version: 1
top level: BBa_K404154
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K404154/annotation2097908/range2097908/1

https://synbiohub.org/public/igem/BBa_K404154/annotation2097908/range2097908/1
type: Range
persistent identity: range2097908
display id: range2097908
version: 1
direction: direction/forward
top level: BBa_K404154
start: 2
end: 655
orientation: inline

ZEGFR_1907
type: SequenceAnnotation
persistent identity: annotation2097909
display id: annotation2097909
version: 1
top level: BBa_K404154
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404154/annotation2097909/range2097909/1

https://synbiohub.org/public/igem/BBa_K404154/annotation2097909/range2097909/1
type: Range
persistent identity: range2097909
display id: range2097909
version: 1
direction: direction/forward
top level: BBa_K404154
start: 671
end: 844
orientation: inline

HSPG-KO
type: SequenceAnnotation
persistent identity: annotation2101718
display id: annotation2101718
version: 1
top level: BBa_K404154
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K404154/annotation2101718/range2101718/1

https://synbiohub.org/public/igem/BBa_K404154/annotation2101718/range2101718/1
type: Range
persistent identity: range2101718
display id: range2101718
version: 1
direction: direction/forward
top level: BBa_K404154
start: 2189
end: 2191
orientation: inline

HSPG-KO
type: SequenceAnnotation
persistent identity: annotation2101717
display id: annotation2101717
version: 1
top level: BBa_K404154
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K404154/annotation2101717/range2101717/1

https://synbiohub.org/public/igem/BBa_K404154/annotation2101717/range2101717/1
type: Range
persistent identity: range2101717
display id: range2101717
version: 1
direction: direction/forward
top level: BBa_K404154
start: 2198
end: 2200
orientation: inline

BBa_K404206
type: ComponentDefinition
persistent identity: BBa K404206
display id: BBa_K404206
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: ViralBrick-587-His-Tag
creator: Freiburg Bioware 2010
created: 2010-08-27T11:00:00Z
modified: 2015-05-08T01:12:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 7049
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: downstream 453
sequence annotation: upstream 587
sequence annotation: TG Spacer
sequence annotation: His-Tag
sequence annotation: upstream 453
sequence annotation: GLS Spacer
sequence annotation: downstream 587
sequence: https://synbiohub.org/public/igem/BBa_K404206_sequence/1

downstream 453
type: SequenceAnnotation
persistent identity: annotation2078960
display id: annotation2078960
version: 1
top level: BBa_K404206
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404206/annotation2078960/range2078960/1

https://synbiohub.org/public/igem/BBa_K404206/annotation2078960/range2078960/1
type: Range
persistent identity: range2078960
display id: range2078960
version: 1
direction: direction/forward
top level: BBa_K404206
start: 97
end: 105
orientation: inline

upstream 587
type: SequenceAnnotation
persistent identity: annotation2078955
display id: annotation2078955
version: 1
top level: BBa_K404206
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404206/annotation2078955/range2078955/1

https://synbiohub.org/public/igem/BBa_K404206/annotation2078955/range2078955/1
type: Range
persistent identity: range2078955
display id: range2078955
version: 1
direction: direction/forward
top level: BBa_K404206
start: 10
end: 42
orientation: inline

TG Spacer
type: SequenceAnnotation
persistent identity: annotation2078956
display id: annotation2078956
version: 1
top level: BBa_K404206
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404206/annotation2078956/range2078956/1

https://synbiohub.org/public/igem/BBa_K404206/annotation2078956/range2078956/1
type: Range
persistent identity: range2078956
display id: range2078956
version: 1
direction: direction/forward
top level: BBa_K404206
start: 43
end: 48
orientation: inline

His-Tag
type: SequenceAnnotation
persistent identity: annotation2078957
display id: annotation2078957
version: 1
top level: BBa_K404206
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404206/annotation2078957/range2078957/1

https://synbiohub.org/public/igem/BBa_K404206/annotation2078957/range2078957/1
type: Range
persistent identity: range2078957
display id: range2078957
version: 1
direction: direction/forward
top level: BBa_K404206
start: 49
end: 66
orientation: inline

upstream 453
type: SequenceAnnotation
persistent identity: annotation2078954
display id: annotation2078954
version: 1
top level: BBa_K404206
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404206/annotation2078954/range2078954/1

https://synbiohub.org/public/igem/BBa_K404206/annotation2078954/range2078954/1
type: Range
persistent identity: range2078954
display id: range2078954
version: 1
direction: direction/forward
top level: BBa_K404206
start: 1
end: 9
orientation: inline

GLS Spacer
type: SequenceAnnotation
persistent identity: annotation2078958
display id: annotation2078958
version: 1
top level: BBa_K404206
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404206/annotation2078958/range2078958/1

https://synbiohub.org/public/igem/BBa_K404206/annotation2078958/range2078958/1
type: Range
persistent identity: range2078958
display id: range2078958
version: 1
direction: direction/forward
top level: BBa_K404206
start: 67
end: 75
orientation: inline

downstream 587
type: SequenceAnnotation
persistent identity: annotation2078959
display id: annotation2078959
version: 1
top level: BBa_K404206
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404206/annotation2078959/range2078959/1

https://synbiohub.org/public/igem/BBa_K404206/annotation2078959/range2078959/1
type: Range
persistent identity: range2078959
display id: range2078959
version: 1
direction: direction/forward
top level: BBa_K404206
start: 75
end: 96
orientation: inline

BBa_K404236
type: ComponentDefinition
persistent identity: BBa K404236
display id: BBa_K404236
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: [AAV2]Rep-VP123_p5TATAless (ViralBrick 453 RGD-587 HSPG-KO)
creator: Freiburg Bioware 2010
created: 2010-10-24T11:00:00Z
modified: 2015-05-08T01:12:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 6674
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: Rep78
sequence annotation: VP3
sequence annotation: Rep52
sequence annotation: HSPG-KO
sequence annotation: p5TATAless
sequence annotation: RGD
sequence annotation: Rep68
sequence annotation: Rep40
sequence annotation: A-spacer
sequence annotation: VP1
sequence annotation: VP2
sequence: https://synbiohub.org/public/igem/BBa_K404236_sequence/1

Rep78
type: SequenceAnnotation
persistent identity: annotation2098403
display id: annotation2098403
version: 1
top level: BBa_K404236
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404236/annotation2098403/range2098403/1

https://synbiohub.org/public/igem/BBa_K404236/annotation2098403/range2098403/1
type: Range
persistent identity: range2098403
display id: range2098403
version: 1
direction: direction/forward
top level: BBa_K404236
start: 2
end: 1867
orientation: inline

VP3
type: SequenceAnnotation
persistent identity: annotation2098410
display id: annotation2098410
version: 1
top level: BBa_K404236
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404236/annotation2098410/range2098410/1

https://synbiohub.org/public/igem/BBa_K404236/annotation2098410/range2098410/1
type: Range
persistent identity: range2098410
display id: range2098410
version: 1
direction: direction/forward
top level: BBa_K404236
start: 2489
end: 4123
orientation: inline

Rep52
type: SequenceAnnotation
persistent identity: annotation2098406
display id: annotation2098406
version: 1
top level: BBa_K404236
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404236/annotation2098406/range2098406/1

https://synbiohub.org/public/igem/BBa_K404236/annotation2098406/range2098406/1
type: Range
persistent identity: range2098406
display id: range2098406
version: 1
direction: direction/forward
top level: BBa_K404236
start: 673
end: 1867
orientation: inline

HSPG-KO
type: SequenceAnnotation
persistent identity: annotation2101873
display id: annotation2101873
version: 1
top level: BBa_K404236
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K404236/annotation2101873/range2101873/1

https://synbiohub.org/public/igem/BBa_K404236/annotation2101873/range2101873/1
type: Range
persistent identity: range2101873
display id: range2101873
version: 1
direction: direction/forward
top level: BBa_K404236
start: 3308
end: 3310
orientation: inline

p5TATAless
type: SequenceAnnotation
persistent identity: annotation2098429
display id: annotation2098429
version: 1
top level: BBa_K404236
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K404236/annotation2098429/range2098429/1

https://synbiohub.org/public/igem/BBa_K404236/annotation2098429/range2098429/1
type: Range
persistent identity: range2098429
display id: range2098429
version: 1
direction: direction/forward
top level: BBa_K404236
start: 4255
end: 4418
orientation: inline

RGD
type: SequenceAnnotation
persistent identity: annotation2098411
display id: annotation2098411
version: 1
top level: BBa_K404236
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404236/annotation2098411/range2098411/1

https://synbiohub.org/public/igem/BBa_K404236/annotation2098411/range2098411/1
type: Range
persistent identity: range2098411
display id: range2098411
version: 1
direction: direction/forward
top level: BBa_K404236
start: 3245
end: 3271
orientation: inline

Rep68
type: SequenceAnnotation
persistent identity: annotation2098405
display id: annotation2098405
version: 1
top level: BBa_K404236
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404236/annotation2098405/range2098405/1

https://synbiohub.org/public/igem/BBa_K404236/annotation2098405/range2098405/1
type: Range
persistent identity: range2098405
display id: range2098405
version: 1
direction: direction/forward
top level: BBa_K404236
start: 2
end: 1933
orientation: inline

Rep40
type: SequenceAnnotation
persistent identity: annotation2098407
display id: annotation2098407
version: 1
top level: BBa_K404236
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404236/annotation2098407/range2098407/1

https://synbiohub.org/public/igem/BBa_K404236/annotation2098407/range2098407/1
type: Range
persistent identity: range2098407
display id: range2098407
version: 1
direction: direction/forward
top level: BBa_K404236
start: 673
end: 1933
orientation: inline

A-spacer
type: SequenceAnnotation
persistent identity: annotation2098413
display id: annotation2098413
version: 1
top level: BBa_K404236
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404236/annotation2098413/range2098413/1

https://synbiohub.org/public/igem/BBa_K404236/annotation2098413/range2098413/1
type: Range
persistent identity: range2098413
display id: range2098413
version: 1
direction: direction/forward
top level: BBa_K404236
start: 3272
end: 3274
orientation: inline

HSPG-KO
type: SequenceAnnotation
persistent identity: annotation2101874
display id: annotation2101874
version: 1
top level: BBa_K404236
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K404236/annotation2101874/range2101874/1

https://synbiohub.org/public/igem/BBa_K404236/annotation2101874/range2101874/1
type: Range
persistent identity: range2101874
display id: range2101874
version: 1
direction: direction/forward
top level: BBa_K404236
start: 3155
end: 3157
orientation: inline

A-spacer
type: SequenceAnnotation
persistent identity: annotation2098412
display id: annotation2098412
version: 1
top level: BBa_K404236
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404236/annotation2098412/range2098412/1

https://synbiohub.org/public/igem/BBa_K404236/annotation2098412/range2098412/1
type: Range
persistent identity: range2098412
display id: range2098412
version: 1
direction: direction/forward
top level: BBa_K404236
start: 3242
end: 3244
orientation: inline

VP1
type: SequenceAnnotation
persistent identity: annotation2098408
display id: annotation2098408
version: 1
top level: BBa_K404236
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404236/annotation2098408/range2098408/1

https://synbiohub.org/public/igem/BBa_K404236/annotation2098408/range2098408/1
type: Range
persistent identity: range2098408
display id: range2098408
version: 1
direction: direction/forward
top level: BBa_K404236
start: 1883
end: 4123
orientation: inline

VP2
type: SequenceAnnotation
persistent identity: annotation2098409
display id: annotation2098409
version: 1
top level: BBa_K404236
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404236/annotation2098409/range2098409/1

https://synbiohub.org/public/igem/BBa_K404236/annotation2098409/range2098409/1
type: Range
persistent identity: range2098409
display id: range2098409
version: 1
direction: direction/forward
top level: BBa_K404236
start: 2294
end: 4123
orientation: inline

BBa_J52011
type: ComponentDefinition
persistent identity: BBa J52011
display id: BBa_J52011
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: dnMyD88-linker-Rluc
creator: Monika Ciglic
created: 2006-10-13T11:00:00Z
modified: 2015-08-31T03:54:04Z
discontinued: false
dominant: false
group u list: _80_
m user id: 0
owner id: 800
owning group id: 80
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: dnMyD88 is a dominant negative form of protein MyD88 (an important protein in signaling cascade through TLR4 receptor), which was our main target for modifying the signaling path. dnMyD88 is linked to Rluc (which acts as a reporter) through six aminoacid long protein linker.
mutable notes: dnMyD88-linker-Rluc was made by using PCR ligation technique. This part is cloned in BioBrick vector pSB1AK3.
mutable provenance: -
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: dnMyD88-linker-Rluc
sequence annotation: dnMyD88
sequence annotation: linker
sequence annotation: Rluc
sequence: https://synbiohub.org/public/igem/BBa_J52011_sequence/1

dnMyD88-linker-Rluc
type: SequenceAnnotation
persistent identity: annotation1902846
display id: annotation1902846
version: 1
top level: BBa_J52011
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_J52011/annotation1902846/range1902846/1

https://synbiohub.org/public/igem/BBa_J52011/annotation1902846/range1902846/1
type: Range
persistent identity: range1902846
display id: range1902846
version: 1
direction: direction/forward
top level: BBa_J52011
start: 1
end: 1371
orientation: inline

dnMyD88
type: SequenceAnnotation
persistent identity: annotation1902834
display id: annotation1902834
version: 1
top level: BBa_J52011
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J52011/annotation1902834/range1902834/1

https://synbiohub.org/public/igem/BBa_J52011/annotation1902834/range1902834/1
type: Range
persistent identity: range1902834
display id: range1902834
version: 1
direction: direction/forward
top level: BBa_J52011
start: 1
end: 417
orientation: inline

linker
type: SequenceAnnotation
persistent identity: annotation1902835
display id: annotation1902835
version: 1
top level: BBa_J52011
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_J52011/annotation1902835/range1902835/1

https://synbiohub.org/public/igem/BBa_J52011/annotation1902835/range1902835/1
type: Range
persistent identity: range1902835
display id: range1902835
version: 1
direction: direction/forward
top level: BBa_J52011
start: 418
end: 435
orientation: inline

Rluc
type: SequenceAnnotation
persistent identity: annotation1902836
display id: annotation1902836
version: 1
top level: BBa_J52011
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_J52011/annotation1902836/range1902836/1

https://synbiohub.org/public/igem/BBa_J52011/annotation1902836/range1902836/1
type: Range
persistent identity: range1902836
display id: range1902836
version: 1
direction: direction/forward
top level: BBa_J52011
start: 436
end: 1371
orientation: inline

BBa_K678005
type: ComponentDefinition
persistent identity: BBa K678005
display id: BBa_K678005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: SV40, mammalian promoter
creator: DTU-Denmark-2
created: 2011-09-03T11:00:00Z
modified: 2015-05-08T01:13:04Z
discontinued: false
dominant: false
experience: experience/None
group u list: _882_
m user id: 0
owner id: 8341
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Promoter for expression in mammalian cells. The SV40 promoter contains an ori.
mutable notes: Compatible with the Plug'n'Play assembly standard
mutable provenance: Plasmid pcDNA3.1 from Invitrogen
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: Linker 1
sequence annotation: SV40 + ori
sequence: https://synbiohub.org/public/igem/BBa_K678005_sequence/1

Linker 1
type: SequenceAnnotation
persistent identity: annotation2127347
display id: annotation2127347
version: 1
top level: BBa_K678005
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K678005/annotation2127347/range2127347/1

https://synbiohub.org/public/igem/BBa_K678005/annotation2127347/range2127347/1
type: Range
persistent identity: range2127347
display id: range2127347
version: 1
direction: direction/forward
top level: BBa_K678005
start: 1
end: 8
orientation: inline

SV40 + ori
type: SequenceAnnotation
persistent identity: annotation2127346
display id: annotation2127346
version: 1
top level: BBa_K678005
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K678005/annotation2127346/range2127346/1

https://synbiohub.org/public/igem/BBa_K678005/annotation2127346/range2127346/1
type: Range
persistent identity: range2127346
display id: range2127346
version: 1
direction: direction/forward
top level: BBa_K678005
start: 9
end: 352
orientation: inline

BBa_K404003
type: ComponentDefinition
persistent identity: BBa K404003
display id: BBa_K404003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: [AAV2]-Rep-VP123(ViralBrick-587KO-empty)_p5-TATAless
creator: Freiburg Bioware 2010
created: 2010-10-20T11:00:00Z
modified: 2015-05-08T01:12:23Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 6674
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: basic region 4
sequence annotation: Rep68
sequence annotation: basic region 2
sequence annotation: scar
sequence annotation: AAV-2-cap
sequence annotation: PLA2
sequence annotation: VP3
sequence annotation: Rep40
sequence annotation: HSPG-KO
sequence annotation: p5TATAless
sequence annotation: A20-1
sequence annotation: VP2
sequence annotation: A20-4
sequence annotation: p19
sequence annotation: VP1
sequence annotation: A20-2
sequence annotation: basic region 1
sequence annotation: AAP
sequence annotation: B1
sequence annotation: p40
sequence annotation: Rep78
sequence annotation: basic region 3
sequence annotation: Rep52
sequence: https://synbiohub.org/public/igem/BBa_K404003_sequence/1

basic region 4
type: SequenceAnnotation
persistent identity: annotation2102707
display id: annotation2102707
version: 1
top level: BBa_K404003
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404003/annotation2102707/range2102707/1

https://synbiohub.org/public/igem/BBa_K404003/annotation2102707/range2102707/1
type: Range
persistent identity: range2102707
display id: range2102707
version: 1
direction: direction/forward
top level: BBa_K404003
start: 2801
end: 2818
orientation: inline

Rep68
type: SequenceAnnotation
persistent identity: annotation2102694
display id: annotation2102694
version: 1
top level: BBa_K404003
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K404003/annotation2102694/range2102694/1

https://synbiohub.org/public/igem/BBa_K404003/annotation2102694/range2102694/1
type: Range
persistent identity: range2102694
display id: range2102694
version: 1
direction: direction/forward
top level: BBa_K404003
start: 1
end: 1933
orientation: inline

basic region 2
type: SequenceAnnotation
persistent identity: annotation2102703
display id: annotation2102703
version: 1
top level: BBa_K404003
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404003/annotation2102703/range2102703/1

https://synbiohub.org/public/igem/BBa_K404003/annotation2102703/range2102703/1
type: Range
persistent identity: range2102703
display id: range2102703
version: 1
direction: direction/forward
top level: BBa_K404003
start: 2300
end: 2320
orientation: inline

scar
type: SequenceAnnotation
persistent identity: annotation2102750
display id: annotation2102750
version: 1
top level: BBa_K404003
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404003/annotation2102750/range2102750/1

https://synbiohub.org/public/igem/BBa_K404003/annotation2102750/range2102750/1
type: Range
persistent identity: range2102750
display id: range2102750
version: 1
direction: direction/forward
top level: BBa_K404003
start: 4214
end: 4221
orientation: inline

AAV-2-cap
type: SequenceAnnotation
persistent identity: annotation2102749
display id: annotation2102749
version: 1
top level: BBa_K404003
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K404003/annotation2102749/range2102749/1

https://synbiohub.org/public/igem/BBa_K404003/annotation2102749/range2102749/1
type: Range
persistent identity: range2102749
display id: range2102749
version: 1
direction: direction/forward
top level: BBa_K404003
start: 3694
end: 4213
orientation: inline

PLA2
type: SequenceAnnotation
persistent identity: annotation2102700
display id: annotation2102700
version: 1
top level: BBa_K404003
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404003/annotation2102700/range2102700/1

https://synbiohub.org/public/igem/BBa_K404003/annotation2102700/range2102700/1
type: Range
persistent identity: range2102700
display id: range2102700
version: 1
direction: direction/forward
top level: BBa_K404003
start: 2015
end: 2191
orientation: inline

VP3
type: SequenceAnnotation
persistent identity: annotation2102705
display id: annotation2102705
version: 1
top level: BBa_K404003
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K404003/annotation2102705/range2102705/1

https://synbiohub.org/public/igem/BBa_K404003/annotation2102705/range2102705/1
type: Range
persistent identity: range2102705
display id: range2102705
version: 1
direction: direction/forward
top level: BBa_K404003
start: 2489
end: 4090
orientation: inline

Rep40
type: SequenceAnnotation
persistent identity: annotation2102697
display id: annotation2102697
version: 1
top level: BBa_K404003
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K404003/annotation2102697/range2102697/1

https://synbiohub.org/public/igem/BBa_K404003/annotation2102697/range2102697/1
type: Range
persistent identity: range2102697
display id: range2102697
version: 1
direction: direction/forward
top level: BBa_K404003
start: 673
end: 1933
orientation: inline

HSPG-KO
type: SequenceAnnotation
persistent identity: annotation2102711
display id: annotation2102711
version: 1
top level: BBa_K404003
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K404003/annotation2102711/range2102711/1

https://synbiohub.org/public/igem/BBa_K404003/annotation2102711/range2102711/1
type: Range
persistent identity: range2102711
display id: range2102711
version: 1
direction: direction/forward
top level: BBa_K404003
start: 3644
end: 3646
orientation: inline

p5TATAless
type: SequenceAnnotation
persistent identity: annotation2102712
display id: annotation2102712
version: 1
top level: BBa_K404003
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K404003/annotation2102712/range2102712/1

https://synbiohub.org/public/igem/BBa_K404003/annotation2102712/range2102712/1
type: Range
persistent identity: range2102712
display id: range2102712
version: 1
direction: direction/forward
top level: BBa_K404003
start: 4222
end: 4385
orientation: inline

A20-1
type: SequenceAnnotation
persistent identity: annotation2102706
display id: annotation2102706
version: 1
top level: BBa_K404003
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K404003/annotation2102706/range2102706/1

https://synbiohub.org/public/igem/BBa_K404003/annotation2102706/range2102706/1
type: Range
persistent identity: range2102706
display id: range2102706
version: 1
direction: direction/forward
top level: BBa_K404003
start: 2693
end: 2722
orientation: inline

VP2
type: SequenceAnnotation
persistent identity: annotation2102702
display id: annotation2102702
version: 1
top level: BBa_K404003
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K404003/annotation2102702/range2102702/1

https://synbiohub.org/public/igem/BBa_K404003/annotation2102702/range2102702/1
type: Range
persistent identity: range2102702
display id: range2102702
version: 1
direction: direction/forward
top level: BBa_K404003
start: 2294
end: 4090
orientation: inline

A20-4
type: SequenceAnnotation
persistent identity: annotation2102709
display id: annotation2102709
version: 1
top level: BBa_K404003
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K404003/annotation2102709/range2102709/1

https://synbiohub.org/public/igem/BBa_K404003/annotation2102709/range2102709/1
type: Range
persistent identity: range2102709
display id: range2102709
version: 1
direction: direction/forward
top level: BBa_K404003
start: 3578
end: 3607
orientation: inline

p19
type: SequenceAnnotation
persistent identity: annotation2102695
display id: annotation2102695
version: 1
top level: BBa_K404003
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K404003/annotation2102695/range2102695/1

https://synbiohub.org/public/igem/BBa_K404003/annotation2102695/range2102695/1
type: Range
persistent identity: range2102695
display id: range2102695
version: 1
direction: direction/forward
top level: BBa_K404003
start: 410
end: 529
orientation: inline

VP1
type: SequenceAnnotation
persistent identity: annotation2102699
display id: annotation2102699
version: 1
top level: BBa_K404003
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K404003/annotation2102699/range2102699/1

https://synbiohub.org/public/igem/BBa_K404003/annotation2102699/range2102699/1
type: Range
persistent identity: range2102699
display id: range2102699
version: 1
direction: direction/forward
top level: BBa_K404003
start: 1833
end: 4090
orientation: inline

A20-2
type: SequenceAnnotation
persistent identity: annotation2102708
display id: annotation2102708
version: 1
top level: BBa_K404003
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K404003/annotation2102708/range2102708/1

https://synbiohub.org/public/igem/BBa_K404003/annotation2102708/range2102708/1
type: Range
persistent identity: range2102708
display id: range2102708
version: 1
direction: direction/forward
top level: BBa_K404003
start: 2990
end: 3016
orientation: inline

basic region 1
type: SequenceAnnotation
persistent identity: annotation2102701
display id: annotation2102701
version: 1
top level: BBa_K404003
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404003/annotation2102701/range2102701/1

https://synbiohub.org/public/igem/BBa_K404003/annotation2102701/range2102701/1
type: Range
persistent identity: range2102701
display id: range2102701
version: 1
direction: direction/forward
top level: BBa_K404003
start: 2240
end: 2260
orientation: inline

AAP
type: SequenceAnnotation
persistent identity: annotation2102747
display id: annotation2102747
version: 1
top level: BBa_K404003
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K404003/annotation2102747/range2102747/1

https://synbiohub.org/public/igem/BBa_K404003/annotation2102747/range2102747/1
type: Range
persistent identity: range2102747
display id: range2102747
version: 1
direction: direction/forward
top level: BBa_K404003
start: 2409
end: 3023
orientation: inline

B1
type: SequenceAnnotation
persistent identity: annotation2102713
display id: annotation2102713
version: 1
top level: BBa_K404003
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K404003/annotation2102713/range2102713/1

https://synbiohub.org/public/igem/BBa_K404003/annotation2102713/range2102713/1
type: Range
persistent identity: range2102713
display id: range2102713
version: 1
direction: direction/forward
top level: BBa_K404003
start: 4058
end: 4081
orientation: inline

p40
type: SequenceAnnotation
persistent identity: annotation2102698
display id: annotation2102698
version: 1
top level: BBa_K404003
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K404003/annotation2102698/range2102698/1

https://synbiohub.org/public/igem/BBa_K404003/annotation2102698/range2102698/1
type: Range
persistent identity: range2102698
display id: range2102698
version: 1
direction: direction/forward
top level: BBa_K404003
start: 1390
end: 1507
orientation: inline

Rep78
type: SequenceAnnotation
persistent identity: annotation2102693
display id: annotation2102693
version: 1
top level: BBa_K404003
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K404003/annotation2102693/range2102693/1

https://synbiohub.org/public/igem/BBa_K404003/annotation2102693/range2102693/1
type: Range
persistent identity: range2102693
display id: range2102693
version: 1
direction: direction/forward
top level: BBa_K404003
start: 1
end: 1867
orientation: inline

basic region 3
type: SequenceAnnotation
persistent identity: annotation2102704
display id: annotation2102704
version: 1
top level: BBa_K404003
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404003/annotation2102704/range2102704/1

https://synbiohub.org/public/igem/BBa_K404003/annotation2102704/range2102704/1
type: Range
persistent identity: range2102704
display id: range2102704
version: 1
direction: direction/forward
top level: BBa_K404003
start: 2378
end: 2398
orientation: inline

Rep52
type: SequenceAnnotation
persistent identity: annotation2102696
display id: annotation2102696
version: 1
top level: BBa_K404003
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K404003/annotation2102696/range2102696/1

https://synbiohub.org/public/igem/BBa_K404003/annotation2102696/range2102696/1
type: Range
persistent identity: range2102696
display id: range2102696
version: 1
direction: direction/forward
top level: BBa_K404003
start: 673
end: 1867
orientation: inline

HSPG-KO
type: SequenceAnnotation
persistent identity: annotation2102710
display id: annotation2102710
version: 1
top level: BBa_K404003
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K404003/annotation2102710/range2102710/1

https://synbiohub.org/public/igem/BBa_K404003/annotation2102710/range2102710/1
type: Range
persistent identity: range2102710
display id: range2102710
version: 1
direction: direction/forward
top level: BBa_K404003
start: 3635
end: 3637
orientation: inline

BBa_K404225
type: ComponentDefinition
persistent identity: BBa K404225
display id: BBa_K404225
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: [AAV2]-Rep-VP123_P5-TATAless (ViralBrick-587-BAP)
creator: Freiburg Bioware 2010
created: 2010-08-27T11:00:00Z
modified: 2015-05-08T01:12:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 7049
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: p5 TATAless
sequence annotation: PLA2
sequence annotation: Rep 68
sequence annotation: Rep78
sequence annotation: TG-Spacer
sequence annotation: AAV-2 cap Stratagene
sequence annotation: BAP
sequence annotation: Rep 52
sequence annotation: VP 1
sequence annotation: GLS-Spacer
sequence annotation: VP 3
sequence annotation: VP 2
sequence annotation: Rep 40
sequence: https://synbiohub.org/public/igem/BBa_K404225_sequence/1

p5 TATAless
type: SequenceAnnotation
persistent identity: annotation2099022
display id: annotation2099022
version: 1
top level: BBa_K404225
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404225/annotation2099022/range2099022/1

https://synbiohub.org/public/igem/BBa_K404225/annotation2099022/range2099022/1
type: Range
persistent identity: range2099022
display id: range2099022
version: 1
direction: direction/forward
top level: BBa_K404225
start: 4282
end: 4445
orientation: inline

PLA2
type: SequenceAnnotation
persistent identity: annotation2099005
display id: annotation2099005
version: 1
top level: BBa_K404225
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404225/annotation2099005/range2099005/1

https://synbiohub.org/public/igem/BBa_K404225/annotation2099005/range2099005/1
type: Range
persistent identity: range2099005
display id: range2099005
version: 1
direction: direction/forward
top level: BBa_K404225
start: 2015
end: 2191
orientation: inline

Rep 68
type: SequenceAnnotation
persistent identity: annotation2098997
display id: annotation2098997
version: 1
top level: BBa_K404225
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404225/annotation2098997/range2098997/1

https://synbiohub.org/public/igem/BBa_K404225/annotation2098997/range2098997/1
type: Range
persistent identity: range2098997
display id: range2098997
version: 1
direction: direction/forward
top level: BBa_K404225
start: 2
end: 1933
orientation: inline

Rep78
type: SequenceAnnotation
persistent identity: annotation2098996
display id: annotation2098996
version: 1
top level: BBa_K404225
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404225/annotation2098996/range2098996/1

https://synbiohub.org/public/igem/BBa_K404225/annotation2098996/range2098996/1
type: Range
persistent identity: range2098996
display id: range2098996
version: 1
direction: direction/forward
top level: BBa_K404225
start: 2
end: 1867
orientation: inline

TG-Spacer
type: SequenceAnnotation
persistent identity: annotation2099026
display id: annotation2099026
version: 1
top level: BBa_K404225
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404225/annotation2099026/range2099026/1

https://synbiohub.org/public/igem/BBa_K404225/annotation2099026/range2099026/1
type: Range
persistent identity: range2099026
display id: range2099026
version: 1
direction: direction/forward
top level: BBa_K404225
start: 3644
end: 3649
orientation: inline

AAV-2 cap Stratagene
type: SequenceAnnotation
persistent identity: annotation2099006
display id: annotation2099006
version: 1
top level: BBa_K404225
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404225/annotation2099006/range2099006/1

https://synbiohub.org/public/igem/BBa_K404225/annotation2099006/range2099006/1
type: Range
persistent identity: range2099006
display id: range2099006
version: 1
direction: direction/forward
top level: BBa_K404225
start: 1883
end: 3753
orientation: inline

BAP
type: SequenceAnnotation
persistent identity: annotation2099025
display id: annotation2099025
version: 1
top level: BBa_K404225
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404225/annotation2099025/range2099025/1

https://synbiohub.org/public/igem/BBa_K404225/annotation2099025/range2099025/1
type: Range
persistent identity: range2099025
display id: range2099025
version: 1
direction: direction/forward
top level: BBa_K404225
start: 3650
end: 3694
orientation: inline

Rep 52
type: SequenceAnnotation
persistent identity: annotation2099003
display id: annotation2099003
version: 1
top level: BBa_K404225
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404225/annotation2099003/range2099003/1

https://synbiohub.org/public/igem/BBa_K404225/annotation2099003/range2099003/1
type: Range
persistent identity: range2099003
display id: range2099003
version: 1
direction: direction/forward
top level: BBa_K404225
start: 673
end: 1867
orientation: inline

VP 1
type: SequenceAnnotation
persistent identity: annotation2099011
display id: annotation2099011
version: 1
top level: BBa_K404225
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404225/annotation2099011/range2099011/1

https://synbiohub.org/public/igem/BBa_K404225/annotation2099011/range2099011/1
type: Range
persistent identity: range2099011
display id: range2099011
version: 1
direction: direction/forward
top level: BBa_K404225
start: 1883
end: 4150
orientation: inline

GLS-Spacer
type: SequenceAnnotation
persistent identity: annotation2099027
display id: annotation2099027
version: 1
top level: BBa_K404225
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404225/annotation2099027/range2099027/1

https://synbiohub.org/public/igem/BBa_K404225/annotation2099027/range2099027/1
type: Range
persistent identity: range2099027
display id: range2099027
version: 1
direction: direction/forward
top level: BBa_K404225
start: 3695
end: 3703
orientation: inline

VP 3
type: SequenceAnnotation
persistent identity: annotation2099020
display id: annotation2099020
version: 1
top level: BBa_K404225
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404225/annotation2099020/range2099020/1

https://synbiohub.org/public/igem/BBa_K404225/annotation2099020/range2099020/1
type: Range
persistent identity: range2099020
display id: range2099020
version: 1
direction: direction/forward
top level: BBa_K404225
start: 2489
end: 4150
orientation: inline

VP 2
type: SequenceAnnotation
persistent identity: annotation2099017
display id: annotation2099017
version: 1
top level: BBa_K404225
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404225/annotation2099017/range2099017/1

https://synbiohub.org/public/igem/BBa_K404225/annotation2099017/range2099017/1
type: Range
persistent identity: range2099017
display id: range2099017
version: 1
direction: direction/forward
top level: BBa_K404225
start: 2294
end: 4150
orientation: inline

Rep 40
type: SequenceAnnotation
persistent identity: annotation2099004
display id: annotation2099004
version: 1
top level: BBa_K404225
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404225/annotation2099004/range2099004/1

https://synbiohub.org/public/igem/BBa_K404225/annotation2099004/range2099004/1
type: Range
persistent identity: range2099004
display id: range2099004
version: 1
direction: direction/forward
top level: BBa_K404225
start: 673
end: 1933
orientation: inline

BBa_K404094
type: ComponentDefinition
persistent identity: BBa K404094
display id: BBa_K404094
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: [AAV2]-AAPex
creator: Freiburg Bioware 2010
created: 2010-08-27T11:00:00Z
modified: 2015-05-08T01:12:23Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 7049
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: misc
sequence: https://synbiohub.org/public/igem/BBa_K404094_sequence/1

misc
type: SequenceAnnotation
persistent identity: annotation2093164
display id: annotation2093164
version: 1
top level: BBa_K404094
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404094/annotation2093164/range2093164/1

https://synbiohub.org/public/igem/BBa_K404094/annotation2093164/range2093164/1
type: Range
persistent identity: range2093164
display id: range2093164
version: 1
direction: direction/forward
top level: BBa_K404094
start: 1
end: 616
orientation: inline

BBa_K404122
type: ComponentDefinition
persistent identity: BBa K404122
display id: BBa_K404122
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: [AAV2]-left-ITR_pCMV_betaglobin_mGMK_TK30_hGH_[AAV2]-right-ITR
creator: Freiburg Bioware 2010
created: 2010-10-10T11:00:00Z
modified: 2015-05-08T01:12:23Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 6733
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: scar
sequence annotation: right ITR
sequence annotation: part of human beta-globin-exon III
sequence annotation: part of chimeric CMV promoter
sequence annotation: beta-globin-intron II
sequence annotation: Beta-Globin-intron
sequence annotation: hGH
sequence annotation: cleavage site
sequence annotation: RBE (Rep-binding element)
sequence annotation: left ITR
sequence annotation: intron 2 of human beta-globin
sequence annotation: polyA
sequence annotation: CMV
sequence annotation: DSE (downstream sequence element)
sequence annotation: mGMK_TK30
sequence: https://synbiohub.org/public/igem/BBa_K404122_sequence/1

scar
type: SequenceAnnotation
persistent identity: annotation2101670
display id: annotation2101670
version: 1
top level: BBa_K404122
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404122/annotation2101670/range2101670/1

https://synbiohub.org/public/igem/BBa_K404122/annotation2101670/range2101670/1
type: Range
persistent identity: range2101670
display id: range2101670
version: 1
direction: direction/forward
top level: BBa_K404122
start: 809
end: 816
orientation: inline

right ITR
type: SequenceAnnotation
persistent identity: annotation2101628
display id: annotation2101628
version: 1
top level: BBa_K404122
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404122/annotation2101628/range2101628/1

https://synbiohub.org/public/igem/BBa_K404122/annotation2101628/range2101628/1
type: Range
persistent identity: range2101628
display id: range2101628
version: 1
direction: direction/forward
top level: BBa_K404122
start: 3521
end: 3658
orientation: inline

part of human beta-globin-exon III
type: SequenceAnnotation
persistent identity: annotation2101622
display id: annotation2101622
version: 1
top level: BBa_K404122
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K404122/annotation2101622/range2101622/1

https://synbiohub.org/public/igem/BBa_K404122/annotation2101622/range2101622/1
type: Range
persistent identity: range2101622
display id: range2101622
version: 1
direction: direction/forward
top level: BBa_K404122
start: 1252
end: 1304
orientation: inline

scar
type: SequenceAnnotation
persistent identity: annotation2101674
display id: annotation2101674
version: 1
top level: BBa_K404122
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404122/annotation2101674/range2101674/1

https://synbiohub.org/public/igem/BBa_K404122/annotation2101674/range2101674/1
type: Range
persistent identity: range2101674
display id: range2101674
version: 1
direction: direction/forward
top level: BBa_K404122
start: 3507
end: 3520
orientation: inline

scar
type: SequenceAnnotation
persistent identity: annotation2101673
display id: annotation2101673
version: 1
top level: BBa_K404122
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404122/annotation2101673/range2101673/1

https://synbiohub.org/public/igem/BBa_K404122/annotation2101673/range2101673/1
type: Range
persistent identity: range2101673
display id: range2101673
version: 1
direction: direction/forward
top level: BBa_K404122
start: 3020
end: 3027
orientation: inline

part of chimeric CMV promoter
type: SequenceAnnotation
persistent identity: annotation2101619
display id: annotation2101619
version: 1
top level: BBa_K404122
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K404122/annotation2101619/range2101619/1

https://synbiohub.org/public/igem/BBa_K404122/annotation2101619/range2101619/1
type: Range
persistent identity: range2101619
display id: range2101619
version: 1
direction: direction/forward
top level: BBa_K404122
start: 824
end: 904
orientation: inline

beta-globin-intron II
type: SequenceAnnotation
persistent identity: annotation2101621
display id: annotation2101621
version: 1
top level: BBa_K404122
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404122/annotation2101621/range2101621/1

https://synbiohub.org/public/igem/BBa_K404122/annotation2101621/range2101621/1
type: Range
persistent identity: range2101621
display id: range2101621
version: 1
direction: direction/forward
top level: BBa_K404122
start: 905
end: 1251
orientation: inline

Beta-Globin-intron
type: SequenceAnnotation
persistent identity: annotation2101618
display id: annotation2101618
version: 1
top level: BBa_K404122
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404122/annotation2101618/range2101618/1

https://synbiohub.org/public/igem/BBa_K404122/annotation2101618/range2101618/1
type: Range
persistent identity: range2101618
display id: range2101618
version: 1
direction: direction/forward
top level: BBa_K404122
start: 817
end: 1309
orientation: inline

hGH
type: SequenceAnnotation
persistent identity: annotation2101624
display id: annotation2101624
version: 1
top level: BBa_K404122
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404122/annotation2101624/range2101624/1

https://synbiohub.org/public/igem/BBa_K404122/annotation2101624/range2101624/1
type: Range
persistent identity: range2101624
display id: range2101624
version: 1
direction: direction/reverse
top level: BBa_K404122
start: 3028
end: 3506
orientation: inline

cleavage site
type: SequenceAnnotation
persistent identity: annotation2101626
display id: annotation2101626
version: 1
top level: BBa_K404122
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404122/annotation2101626/range2101626/1

https://synbiohub.org/public/igem/BBa_K404122/annotation2101626/range2101626/1
type: Range
persistent identity: range2101626
display id: range2101626
version: 1
direction: direction/reverse
top level: BBa_K404122
start: 3080
end: 3081
orientation: inline

RBE (Rep-binding element)
type: SequenceAnnotation
persistent identity: annotation2101616
display id: annotation2101616
version: 1
top level: BBa_K404122
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K404122/annotation2101616/range2101616/1

https://synbiohub.org/public/igem/BBa_K404122/annotation2101616/range2101616/1
type: Range
persistent identity: range2101616
display id: range2101616
version: 1
direction: direction/forward
top level: BBa_K404122
start: 88
end: 103
orientation: inline

left ITR
type: SequenceAnnotation
persistent identity: annotation2101615
display id: annotation2101615
version: 1
top level: BBa_K404122
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404122/annotation2101615/range2101615/1

https://synbiohub.org/public/igem/BBa_K404122/annotation2101615/range2101615/1
type: Range
persistent identity: range2101615
display id: range2101615
version: 1
direction: direction/forward
top level: BBa_K404122
start: 6
end: 140
orientation: inline

RBE (Rep-binding element)
type: SequenceAnnotation
persistent identity: annotation2101629
display id: annotation2101629
version: 1
top level: BBa_K404122
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K404122/annotation2101629/range2101629/1

https://synbiohub.org/public/igem/BBa_K404122/annotation2101629/range2101629/1
type: Range
persistent identity: range2101629
display id: range2101629
version: 1
direction: direction/forward
top level: BBa_K404122
start: 3632
end: 3647
orientation: inline

intron 2 of human beta-globin
type: SequenceAnnotation
persistent identity: annotation2101620
display id: annotation2101620
version: 1
top level: BBa_K404122
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K404122/annotation2101620/range2101620/1

https://synbiohub.org/public/igem/BBa_K404122/annotation2101620/range2101620/1
type: Range
persistent identity: range2101620
display id: range2101620
version: 1
direction: direction/forward
top level: BBa_K404122
start: 899
end: 1252
orientation: inline

polyA
type: SequenceAnnotation
persistent identity: annotation2101627
display id: annotation2101627
version: 1
top level: BBa_K404122
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_K404122/annotation2101627/range2101627/1

https://synbiohub.org/public/igem/BBa_K404122/annotation2101627/range2101627/1
type: Range
persistent identity: range2101627
display id: range2101627
version: 1
direction: direction/forward
top level: BBa_K404122
start: 3110
end: 3115
orientation: inline

scar
type: SequenceAnnotation
persistent identity: annotation2101669
display id: annotation2101669
version: 1
top level: BBa_K404122
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404122/annotation2101669/range2101669/1

https://synbiohub.org/public/igem/BBa_K404122/annotation2101669/range2101669/1
type: Range
persistent identity: range2101669
display id: range2101669
version: 1
direction: direction/forward
top level: BBa_K404122
start: 1
end: 5
orientation: inline

scar
type: SequenceAnnotation
persistent identity: annotation2101672
display id: annotation2101672
version: 1
top level: BBa_K404122
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404122/annotation2101672/range2101672/1

https://synbiohub.org/public/igem/BBa_K404122/annotation2101672/range2101672/1
type: Range
persistent identity: range2101672
display id: range2101672
version: 1
direction: direction/forward
top level: BBa_K404122
start: 1310
end: 1315
orientation: inline

CMV
type: SequenceAnnotation
persistent identity: annotation2101617
display id: annotation2101617
version: 1
top level: BBa_K404122
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K404122/annotation2101617/range2101617/1

https://synbiohub.org/public/igem/BBa_K404122/annotation2101617/range2101617/1
type: Range
persistent identity: range2101617
display id: range2101617
version: 1
direction: direction/forward
top level: BBa_K404122
start: 155
end: 808
orientation: inline

DSE (downstream sequence element)
type: SequenceAnnotation
persistent identity: annotation2101625
display id: annotation2101625
version: 1
top level: BBa_K404122
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404122/annotation2101625/range2101625/1

https://synbiohub.org/public/igem/BBa_K404122/annotation2101625/range2101625/1
type: Range
persistent identity: range2101625
display id: range2101625
version: 1
direction: direction/reverse
top level: BBa_K404122
start: 3046
end: 3050
orientation: inline

scar
type: SequenceAnnotation
persistent identity: annotation2101668
display id: annotation2101668
version: 1
top level: BBa_K404122
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404122/annotation2101668/range2101668/1

https://synbiohub.org/public/igem/BBa_K404122/annotation2101668/range2101668/1
type: Range
persistent identity: range2101668
display id: range2101668
version: 1
direction: direction/forward
top level: BBa_K404122
start: 141
end: 154
orientation: inline

mGMK_TK30
type: SequenceAnnotation
persistent identity: annotation2101623
display id: annotation2101623
version: 1
top level: BBa_K404122
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K404122/annotation2101623/range2101623/1

https://synbiohub.org/public/igem/BBa_K404122/annotation2101623/range2101623/1
type: Range
persistent identity: range2101623
display id: range2101623
version: 1
direction: direction/forward
top level: BBa_K404122
start: 1316
end: 3019
orientation: inline

BBa_J52021
type: ComponentDefinition
persistent identity: BBa J52021
display id: BBa_J52021
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: dnTraf6-linker-GFP
creator: Matej Skocaj
created: 2006-10-13T11:00:00Z
modified: 2016-01-26T09:38:59Z
discontinued: false
dominant: false
experience: experience/None
group u list: _80_
m user id: 0
m user id: 4206
owner id: 857
owning group id: 80
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Part is composed of TIR domain of Traf6 and GFP linked with 6 aminoacids long linker. GFP serves as reporter. For usage in eukaryotes.
mutable notes: None.
mutable provenance: dnTraf6- pDeNy GFP-
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: dnTraf6-linker-GFP
sequence annotation: GFP
sequence annotation: linker
sequence annotation: dnTraf6
sequence: https://synbiohub.org/public/igem/BBa_J52021_sequence/1

dnTraf6-linker-GFP
type: SequenceAnnotation
persistent identity: annotation1903262
display id: annotation1903262
version: 1
top level: BBa_J52021
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_J52021/annotation1903262/range1903262/1

https://synbiohub.org/public/igem/BBa_J52021/annotation1903262/range1903262/1
type: Range
persistent identity: range1903262
display id: range1903262
version: 1
direction: direction/forward
top level: BBa_J52021
start: 1
end: 1446
orientation: inline

GFP
type: SequenceAnnotation
persistent identity: annotation1903261
display id: annotation1903261
version: 1
top level: BBa_J52021
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J52021/annotation1903261/range1903261/1

https://synbiohub.org/public/igem/BBa_J52021/annotation1903261/range1903261/1
type: Range
persistent identity: range1903261
display id: range1903261
version: 1
direction: direction/forward
top level: BBa_J52021
start: 733
end: 1446
orientation: inline

linker
type: SequenceAnnotation
persistent identity: annotation1903260
display id: annotation1903260
version: 1
top level: BBa_J52021
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_J52021/annotation1903260/range1903260/1

https://synbiohub.org/public/igem/BBa_J52021/annotation1903260/range1903260/1
type: Range
persistent identity: range1903260
display id: range1903260
version: 1
direction: direction/forward
top level: BBa_J52021
start: 715
end: 732
orientation: inline

dnTraf6
type: SequenceAnnotation
persistent identity: annotation1903259
display id: annotation1903259
version: 1
top level: BBa_J52021
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J52021/annotation1903259/range1903259/1

https://synbiohub.org/public/igem/BBa_J52021/annotation1903259/range1903259/1
type: Range
persistent identity: range1903259
display id: range1903259
version: 1
direction: direction/forward
top level: BBa_J52021
start: 1
end: 714
orientation: inline

BBa_K404255
type: ComponentDefinition
persistent identity: BBa K404255
display id: BBa_K404255
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pCMV_[AAV2]-VP123ex (ViralBrick-587KO-Z34C)
creator: Freiburg Bioware 2010
created: 2010-10-20T11:00:00Z
modified: 2015-05-08T01:12:25Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 6674
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: VP2
sequence annotation: upstream 587
sequence annotation: AAV-2-cap (Stratagene)
sequence annotation: downstream 587
sequence annotation: HSPG-KO
sequence annotation: VP1
sequence annotation: Z34C
sequence annotation: VP3
sequence annotation: CMV
sequence: https://synbiohub.org/public/igem/BBa_K404255_sequence/1

VP2
type: SequenceAnnotation
persistent identity: annotation2094720
display id: annotation2094720
version: 1
top level: BBa_K404255
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404255/annotation2094720/range2094720/1

https://synbiohub.org/public/igem/BBa_K404255/annotation2094720/range2094720/1
type: Range
persistent identity: range2094720
display id: range2094720
version: 1
direction: direction/forward
top level: BBa_K404255
start: 1079
end: 2977
orientation: inline

upstream 587
type: SequenceAnnotation
persistent identity: annotation2094724
display id: annotation2094724
version: 1
top level: BBa_K404255
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404255/annotation2094724/range2094724/1

https://synbiohub.org/public/igem/BBa_K404255/annotation2094724/range2094724/1
type: Range
persistent identity: range2094724
display id: range2094724
version: 1
direction: direction/forward
top level: BBa_K404255
start: 2397
end: 2428
orientation: inline

AAV-2-cap (Stratagene)
type: SequenceAnnotation
persistent identity: annotation2094722
display id: annotation2094722
version: 1
top level: BBa_K404255
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404255/annotation2094722/range2094722/1

https://synbiohub.org/public/igem/BBa_K404255/annotation2094722/range2094722/1
type: Range
persistent identity: range2094722
display id: range2094722
version: 1
direction: direction/forward
top level: BBa_K404255
start: 671
end: 3100
orientation: inline

downstream 587
type: SequenceAnnotation
persistent identity: annotation2094725
display id: annotation2094725
version: 1
top level: BBa_K404255
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404255/annotation2094725/range2094725/1

https://synbiohub.org/public/igem/BBa_K404255/annotation2094725/range2094725/1
type: Range
persistent identity: range2094725
display id: range2094725
version: 1
direction: direction/forward
top level: BBa_K404255
start: 2531
end: 2546
orientation: inline

HSPG-KO
type: SequenceAnnotation
persistent identity: annotation2101892
display id: annotation2101892
version: 1
top level: BBa_K404255
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K404255/annotation2101892/range2101892/1

https://synbiohub.org/public/igem/BBa_K404255/annotation2101892/range2101892/1
type: Range
persistent identity: range2101892
display id: range2101892
version: 1
direction: direction/forward
top level: BBa_K404255
start: 2531
end: 2533
orientation: inline

VP1
type: SequenceAnnotation
persistent identity: annotation2094719
display id: annotation2094719
version: 1
top level: BBa_K404255
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404255/annotation2094719/range2094719/1

https://synbiohub.org/public/igem/BBa_K404255/annotation2094719/range2094719/1
type: Range
persistent identity: range2094719
display id: range2094719
version: 1
direction: direction/forward
top level: BBa_K404255
start: 671
end: 2977
orientation: inline

HSPG-KO
type: SequenceAnnotation
persistent identity: annotation2101893
display id: annotation2101893
version: 1
top level: BBa_K404255
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K404255/annotation2101893/range2101893/1

https://synbiohub.org/public/igem/BBa_K404255/annotation2101893/range2101893/1
type: Range
persistent identity: range2101893
display id: range2101893
version: 1
direction: direction/forward
top level: BBa_K404255
start: 2420
end: 2422
orientation: inline

Z34C
type: SequenceAnnotation
persistent identity: annotation2094723
display id: annotation2094723
version: 1
top level: BBa_K404255
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404255/annotation2094723/range2094723/1

https://synbiohub.org/public/igem/BBa_K404255/annotation2094723/range2094723/1
type: Range
persistent identity: range2094723
display id: range2094723
version: 1
direction: direction/forward
top level: BBa_K404255
start: 2429
end: 2530
orientation: inline

VP3
type: SequenceAnnotation
persistent identity: annotation2094721
display id: annotation2094721
version: 1
top level: BBa_K404255
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404255/annotation2094721/range2094721/1

https://synbiohub.org/public/igem/BBa_K404255/annotation2094721/range2094721/1
type: Range
persistent identity: range2094721
display id: range2094721
version: 1
direction: direction/forward
top level: BBa_K404255
start: 1274
end: 2977
orientation: inline

CMV
type: SequenceAnnotation
persistent identity: annotation2094718
display id: annotation2094718
version: 1
top level: BBa_K404255
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K404255/annotation2094718/range2094718/1

https://synbiohub.org/public/igem/BBa_K404255/annotation2094718/range2094718/1
type: Range
persistent identity: range2094718
display id: range2094718
version: 1
direction: direction/forward
top level: BBa_K404255
start: 2
end: 655
orientation: inline

BBa_K404227
type: ComponentDefinition
persistent identity: BBa K404227
display id: BBa_K404227
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: [AAV2]-Rep-VP123_P5-TATAless (ViralBrick-587-RGD)
creator: Freiburg Bioware 2010
created: 2010-08-27T11:00:00Z
modified: 2015-05-08T01:12:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 7049
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: Rep40
sequence annotation: Rep68
sequence annotation: p5TATAless
sequence annotation: Rep78
sequence annotation: downstream 587
sequence annotation: RGD
sequence annotation: TG-spacer
sequence annotation: VP2
sequence annotation: upstream 587
sequence annotation: VP1
sequence annotation: VP3
sequence annotation: GLS-spacer
sequence annotation: Rep52
sequence: https://synbiohub.org/public/igem/BBa_K404227_sequence/1

Rep40
type: SequenceAnnotation
persistent identity: annotation2099010
display id: annotation2099010
version: 1
top level: BBa_K404227
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404227/annotation2099010/range2099010/1

https://synbiohub.org/public/igem/BBa_K404227/annotation2099010/range2099010/1
type: Range
persistent identity: range2099010
display id: range2099010
version: 1
direction: direction/forward
top level: BBa_K404227
start: 673
end: 1933
orientation: inline

Rep68
type: SequenceAnnotation
persistent identity: annotation2099008
display id: annotation2099008
version: 1
top level: BBa_K404227
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404227/annotation2099008/range2099008/1

https://synbiohub.org/public/igem/BBa_K404227/annotation2099008/range2099008/1
type: Range
persistent identity: range2099008
display id: range2099008
version: 1
direction: direction/forward
top level: BBa_K404227
start: 2
end: 1933
orientation: inline

p5TATAless
type: SequenceAnnotation
persistent identity: annotation2099021
display id: annotation2099021
version: 1
top level: BBa_K404227
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K404227/annotation2099021/range2099021/1

https://synbiohub.org/public/igem/BBa_K404227/annotation2099021/range2099021/1
type: Range
persistent identity: range2099021
display id: range2099021
version: 1
direction: direction/forward
top level: BBa_K404227
start: 4264
end: 4427
orientation: inline

Rep78
type: SequenceAnnotation
persistent identity: annotation2099007
display id: annotation2099007
version: 1
top level: BBa_K404227
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404227/annotation2099007/range2099007/1

https://synbiohub.org/public/igem/BBa_K404227/annotation2099007/range2099007/1
type: Range
persistent identity: range2099007
display id: range2099007
version: 1
direction: direction/forward
top level: BBa_K404227
start: 2
end: 1867
orientation: inline

downstream 587
type: SequenceAnnotation
persistent identity: annotation2099030
display id: annotation2099030
version: 1
top level: BBa_K404227
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404227/annotation2099030/range2099030/1

https://synbiohub.org/public/igem/BBa_K404227/annotation2099030/range2099030/1
type: Range
persistent identity: range2099030
display id: range2099030
version: 1
direction: direction/forward
top level: BBa_K404227
start: 3686
end: 3701
orientation: inline

RGD
type: SequenceAnnotation
persistent identity: annotation2099023
display id: annotation2099023
version: 1
top level: BBa_K404227
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404227/annotation2099023/range2099023/1

https://synbiohub.org/public/igem/BBa_K404227/annotation2099023/range2099023/1
type: Range
persistent identity: range2099023
display id: range2099023
version: 1
direction: direction/forward
top level: BBa_K404227
start: 3650
end: 3676
orientation: inline

TG-spacer
type: SequenceAnnotation
persistent identity: annotation2099024
display id: annotation2099024
version: 1
top level: BBa_K404227
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404227/annotation2099024/range2099024/1

https://synbiohub.org/public/igem/BBa_K404227/annotation2099024/range2099024/1
type: Range
persistent identity: range2099024
display id: range2099024
version: 1
direction: direction/forward
top level: BBa_K404227
start: 3644
end: 3649
orientation: inline

VP2
type: SequenceAnnotation
persistent identity: annotation2099018
display id: annotation2099018
version: 1
top level: BBa_K404227
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404227/annotation2099018/range2099018/1

https://synbiohub.org/public/igem/BBa_K404227/annotation2099018/range2099018/1
type: Range
persistent identity: range2099018
display id: range2099018
version: 1
direction: direction/forward
top level: BBa_K404227
start: 2294
end: 4132
orientation: inline

upstream 587
type: SequenceAnnotation
persistent identity: annotation2099029
display id: annotation2099029
version: 1
top level: BBa_K404227
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404227/annotation2099029/range2099029/1

https://synbiohub.org/public/igem/BBa_K404227/annotation2099029/range2099029/1
type: Range
persistent identity: range2099029
display id: range2099029
version: 1
direction: direction/forward
top level: BBa_K404227
start: 3612
end: 3643
orientation: inline

VP1
type: SequenceAnnotation
persistent identity: annotation2099012
display id: annotation2099012
version: 1
top level: BBa_K404227
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404227/annotation2099012/range2099012/1

https://synbiohub.org/public/igem/BBa_K404227/annotation2099012/range2099012/1
type: Range
persistent identity: range2099012
display id: range2099012
version: 1
direction: direction/forward
top level: BBa_K404227
start: 1883
end: 4132
orientation: inline

VP3
type: SequenceAnnotation
persistent identity: annotation2099019
display id: annotation2099019
version: 1
top level: BBa_K404227
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404227/annotation2099019/range2099019/1

https://synbiohub.org/public/igem/BBa_K404227/annotation2099019/range2099019/1
type: Range
persistent identity: range2099019
display id: range2099019
version: 1
direction: direction/forward
top level: BBa_K404227
start: 2489
end: 4132
orientation: inline

GLS-spacer
type: SequenceAnnotation
persistent identity: annotation2099028
display id: annotation2099028
version: 1
top level: BBa_K404227
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404227/annotation2099028/range2099028/1

https://synbiohub.org/public/igem/BBa_K404227/annotation2099028/range2099028/1
type: Range
persistent identity: range2099028
display id: range2099028
version: 1
direction: direction/forward
top level: BBa_K404227
start: 3677
end: 3685
orientation: inline

Rep52
type: SequenceAnnotation
persistent identity: annotation2099009
display id: annotation2099009
version: 1
top level: BBa_K404227
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404227/annotation2099009/range2099009/1

https://synbiohub.org/public/igem/BBa_K404227/annotation2099009/range2099009/1
type: Range
persistent identity: range2099009
display id: range2099009
version: 1
direction: direction/forward
top level: BBa_K404227
start: 673
end: 1867
orientation: inline

His_FluA
type: ComponentDefinition
persistent identity: BBa S04282
display id: BBa_S04282
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: K157011(His affinity tag; Freiburg standard):K157004(Fluorescin-A-binding )
creator: Timo Lorenz
created: 2009-10-04T11:00:00Z
modified: 2015-05-08T01:14:37Z
discontinued: false
dominant: false
experience: experience/None
group u list: _9_
m user id: 0
owner id: 4732
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_K157011
component: BBa_K157004
sequence annotation: BBa_K157004
sequence annotation: BBa_K157011
sequence: https://synbiohub.org/public/igem/BBa_S04282_sequence/1

BBa_K157011
type: Component
persistent identity: component2218738
display id: component2218738
version: 1
top level: His_FluA
access: public
definition: https://synbiohub.org/public/igem/BBa_K157011/1

BBa_K157004
type: Component
persistent identity: component2218740
display id: component2218740
version: 1
top level: His_FluA
access: public
definition: https://synbiohub.org/public/igem/BBa_K157004/1

BBa_K157004
type: SequenceAnnotation
persistent identity: annotation2218740
display id: annotation2218740
version: 1
top level: His_FluA
location: https://synbiohub.org/public/igem/BBa_S04282/annotation2218740/range2218740/1
component: BBa_K157004

https://synbiohub.org/public/igem/BBa_S04282/annotation2218740/range2218740/1
type: Range
persistent identity: range2218740
display id: range2218740
version: 1
direction: direction/reverse
top level: His_FluA
start: 27
end: 548
orientation: inline

BBa_K157011
type: SequenceAnnotation
persistent identity: annotation2218738
display id: annotation2218738
version: 1
top level: His_FluA
location: https://synbiohub.org/public/igem/BBa_S04282/annotation2218738/range2218738/1
component: BBa_K157011

https://synbiohub.org/public/igem/BBa_S04282/annotation2218738/range2218738/1
type: Range
persistent identity: range2218738
display id: range2218738
version: 1
direction: direction/reverse
top level: His_FluA
start: 1
end: 18
orientation: inline

BBa_K404248
type: ComponentDefinition
persistent identity: BBa K404248
display id: BBa_K404248
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pCMV_[AAV2]-VP123ex (ViralBrick-587-RGD)
creator: Freiburg Bioware 2010
created: 2010-10-13T11:00:00Z
modified: 2015-05-08T01:12:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 7049
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: upstream 587
sequence annotation: VP3
sequence annotation: VP2
sequence annotation: VP1
sequence annotation: RGD
sequence annotation: TG-spacer
sequence annotation: AAV-2-cap (Stratagene)
sequence annotation: GLS-spacer
sequence annotation: CMV promoter
sequence annotation: downstream 587
sequence: https://synbiohub.org/public/igem/BBa_K404248_sequence/1

upstream 587
type: SequenceAnnotation
persistent identity: annotation2094390
display id: annotation2094390
version: 1
top level: BBa_K404248
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404248/annotation2094390/range2094390/1

https://synbiohub.org/public/igem/BBa_K404248/annotation2094390/range2094390/1
type: Range
persistent identity: range2094390
display id: range2094390
version: 1
direction: direction/forward
top level: BBa_K404248
start: 2397
end: 2428
orientation: inline

VP3
type: SequenceAnnotation
persistent identity: annotation2094315
display id: annotation2094315
version: 1
top level: BBa_K404248
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404248/annotation2094315/range2094315/1

https://synbiohub.org/public/igem/BBa_K404248/annotation2094315/range2094315/1
type: Range
persistent identity: range2094315
display id: range2094315
version: 1
direction: direction/forward
top level: BBa_K404248
start: 1274
end: 2917
orientation: inline

VP2
type: SequenceAnnotation
persistent identity: annotation2094314
display id: annotation2094314
version: 1
top level: BBa_K404248
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404248/annotation2094314/range2094314/1

https://synbiohub.org/public/igem/BBa_K404248/annotation2094314/range2094314/1
type: Range
persistent identity: range2094314
display id: range2094314
version: 1
direction: direction/forward
top level: BBa_K404248
start: 1079
end: 2917
orientation: inline

VP1
type: SequenceAnnotation
persistent identity: annotation2094295
display id: annotation2094295
version: 1
top level: BBa_K404248
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404248/annotation2094295/range2094295/1

https://synbiohub.org/public/igem/BBa_K404248/annotation2094295/range2094295/1
type: Range
persistent identity: range2094295
display id: range2094295
version: 1
direction: direction/forward
top level: BBa_K404248
start: 671
end: 2917
orientation: inline

RGD
type: SequenceAnnotation
persistent identity: annotation2094371
display id: annotation2094371
version: 1
top level: BBa_K404248
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404248/annotation2094371/range2094371/1

https://synbiohub.org/public/igem/BBa_K404248/annotation2094371/range2094371/1
type: Range
persistent identity: range2094371
display id: range2094371
version: 1
direction: direction/forward
top level: BBa_K404248
start: 2435
end: 2461
orientation: inline

TG-spacer
type: SequenceAnnotation
persistent identity: annotation2094410
display id: annotation2094410
version: 1
top level: BBa_K404248
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404248/annotation2094410/range2094410/1

https://synbiohub.org/public/igem/BBa_K404248/annotation2094410/range2094410/1
type: Range
persistent identity: range2094410
display id: range2094410
version: 1
direction: direction/forward
top level: BBa_K404248
start: 2429
end: 2434
orientation: inline

AAV-2-cap (Stratagene)
type: SequenceAnnotation
persistent identity: annotation2094316
display id: annotation2094316
version: 1
top level: BBa_K404248
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404248/annotation2094316/range2094316/1

https://synbiohub.org/public/igem/BBa_K404248/annotation2094316/range2094316/1
type: Range
persistent identity: range2094316
display id: range2094316
version: 1
direction: direction/forward
top level: BBa_K404248
start: 671
end: 3040
orientation: inline

GLS-spacer
type: SequenceAnnotation
persistent identity: annotation2094429
display id: annotation2094429
version: 1
top level: BBa_K404248
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404248/annotation2094429/range2094429/1

https://synbiohub.org/public/igem/BBa_K404248/annotation2094429/range2094429/1
type: Range
persistent identity: range2094429
display id: range2094429
version: 1
direction: direction/forward
top level: BBa_K404248
start: 2462
end: 2470
orientation: inline

CMV promoter
type: SequenceAnnotation
persistent identity: annotation2094294
display id: annotation2094294
version: 1
top level: BBa_K404248
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K404248/annotation2094294/range2094294/1

https://synbiohub.org/public/igem/BBa_K404248/annotation2094294/range2094294/1
type: Range
persistent identity: range2094294
display id: range2094294
version: 1
direction: direction/forward
top level: BBa_K404248
start: 2
end: 655
orientation: inline

downstream 587
type: SequenceAnnotation
persistent identity: annotation2094391
display id: annotation2094391
version: 1
top level: BBa_K404248
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404248/annotation2094391/range2094391/1

https://synbiohub.org/public/igem/BBa_K404248/annotation2094391/range2094391/1
type: Range
persistent identity: range2094391
display id: range2094391
version: 1
direction: direction/forward
top level: BBa_K404248
start: 2471
end: 2486
orientation: inline

BBa_K1926003
type: ComponentDefinition
persistent identity: BBa K1926003
display id: BBa_K1926003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: A cyclic promoter of CDK4 from human genome
creator: Xinyi Liu
created: 2016-10-08T11:00:00Z
modified: 2016-10-19T01:16:14Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2393_
m user id: 28998
owner id: 28998
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This part is a cyclic promoter named CDK4 from human genome. It can lead to transcription of the downstream DNA sequence in every G1 phase[4]. 4. Guo, Z.Y., et al., The elements of human cyclin D1 promoter and regulation involved. Clin Epigenetics, 2011. 2(2): p. 63-76. You may use this part to: 1) Express something in mammal cell lines particularly in G1 phases or once in every cell cycle by stable transfecting it into cell line; 2) Use it as a human promoter by transient transfecting it into cells.
mutable notes: None
mutable provenance: The sequence was retrieved from Addgene. We got it from human genome through PCR using the following primers: pCDK4-F: TGGTGGAGCGAAAAGGTGACAGCATC pCDK4-R: GCGGAAACTGGGAGGGCGGGGCGAA (Product: 457)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: mutate C into A to remove Pst1
sequence annotation: P CDK4
sequence: https://synbiohub.org/public/igem/BBa_K1926003_sequence/1

mutate C into A to remove Pst1
type: SequenceAnnotation
persistent identity: annotation2491731
display id: annotation2491731
version: 1
top level: BBa_K1926003
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K1926003/annotation2491731/range2491731/1

https://synbiohub.org/public/igem/BBa_K1926003/annotation2491731/range2491731/1
type: Range
persistent identity: range2491731
display id: range2491731
version: 1
direction: direction/forward
top level: BBa_K1926003
start: 284
end: 284
orientation: inline

P CDK4
type: SequenceAnnotation
persistent identity: annotation2491730
display id: annotation2491730
version: 1
top level: BBa_K1926003
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1926003/annotation2491730/range2491730/1

https://synbiohub.org/public/igem/BBa_K1926003/annotation2491730/range2491730/1
type: Range
persistent identity: range2491730
display id: range2491730
version: 1
direction: direction/forward
top level: BBa_K1926003
start: 65
end: 522
orientation: inline

BBa_K404007
type: ComponentDefinition
persistent identity: BBa K404007
display id: BBa_K404007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pCMV_[AAV2]-VP123
creator: Freiburg Bioware 2010
created: 2010-10-20T11:00:00Z
modified: 2015-05-08T01:12:23Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 6674
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: VP3
sequence annotation: BBa_I712004
sequence annotation: CMV
sequence annotation: VP1
sequence annotation: VP2
sequence annotation: AAV-2-cap (Stratagene)
sequence: https://synbiohub.org/public/igem/BBa_K404007_sequence/1

VP3
type: SequenceAnnotation
persistent identity: annotation2099432
display id: annotation2099432
version: 1
top level: BBa_K404007
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404007/annotation2099432/range2099432/1

https://synbiohub.org/public/igem/BBa_K404007/annotation2099432/range2099432/1
type: Range
persistent identity: range2099432
display id: range2099432
version: 1
direction: direction/forward
top level: BBa_K404007
start: 1274
end: 2875
orientation: inline

BBa_I712004
type: SequenceAnnotation
persistent identity: annotation2099428
display id: annotation2099428
version: 1
top level: BBa_K404007
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K404007/annotation2099428/range2099428/1

https://synbiohub.org/public/igem/BBa_K404007/annotation2099428/range2099428/1
type: Range
persistent identity: range2099428
display id: range2099428
version: 1
direction: direction/forward
top level: BBa_K404007
start: 2
end: 655
orientation: inline

CMV
type: SequenceAnnotation
persistent identity: annotation2099427
display id: annotation2099427
version: 1
top level: BBa_K404007
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K404007/annotation2099427/range2099427/1

https://synbiohub.org/public/igem/BBa_K404007/annotation2099427/range2099427/1
type: Range
persistent identity: range2099427
display id: range2099427
version: 1
direction: direction/forward
top level: BBa_K404007
start: 2
end: 655
orientation: inline

VP1
type: SequenceAnnotation
persistent identity: annotation2099429
display id: annotation2099429
version: 1
top level: BBa_K404007
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404007/annotation2099429/range2099429/1

https://synbiohub.org/public/igem/BBa_K404007/annotation2099429/range2099429/1
type: Range
persistent identity: range2099429
display id: range2099429
version: 1
direction: direction/forward
top level: BBa_K404007
start: 671
end: 2875
orientation: inline

VP2
type: SequenceAnnotation
persistent identity: annotation2099431
display id: annotation2099431
version: 1
top level: BBa_K404007
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404007/annotation2099431/range2099431/1

https://synbiohub.org/public/igem/BBa_K404007/annotation2099431/range2099431/1
type: Range
persistent identity: range2099431
display id: range2099431
version: 1
direction: direction/forward
top level: BBa_K404007
start: 1079
end: 2875
orientation: inline

AAV-2-cap (Stratagene)
type: SequenceAnnotation
persistent identity: annotation2099430
display id: annotation2099430
version: 1
top level: BBa_K404007
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404007/annotation2099430/range2099430/1

https://synbiohub.org/public/igem/BBa_K404007/annotation2099430/range2099430/1
type: Range
persistent identity: range2099430
display id: range2099430
version: 1
direction: direction/forward
top level: BBa_K404007
start: 671
end: 2998
orientation: inline

BBa_K404126
type: ComponentDefinition
persistent identity: BBa K404126
display id: BBa_K404126
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: [AAV2]-left-ITR_pCMV_betaglobin_CD_hGH_[AAV2]-right-ITR
creator: Freiburg Bioware 2010
created: 2010-10-10T11:00:00Z
modified: 2015-05-08T01:12:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 6733
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: Cytosine deaminase
sequence annotation: Beta-Globin-intron
sequence annotation: hGH
sequence annotation: right ITR
sequence annotation: left ITR
sequence annotation: CMV promoter
sequence: https://synbiohub.org/public/igem/BBa_K404126_sequence/1

Cytosine deaminase
type: SequenceAnnotation
persistent identity: annotation2093294
display id: annotation2093294
version: 1
top level: BBa_K404126
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404126/annotation2093294/range2093294/1

https://synbiohub.org/public/igem/BBa_K404126/annotation2093294/range2093294/1
type: Range
persistent identity: range2093294
display id: range2093294
version: 1
direction: direction/forward
top level: BBa_K404126
start: 1325
end: 2605
orientation: inline

Beta-Globin-intron
type: SequenceAnnotation
persistent identity: annotation2093293
display id: annotation2093293
version: 1
top level: BBa_K404126
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404126/annotation2093293/range2093293/1

https://synbiohub.org/public/igem/BBa_K404126/annotation2093293/range2093293/1
type: Range
persistent identity: range2093293
display id: range2093293
version: 1
direction: direction/forward
top level: BBa_K404126
start: 816
end: 1310
orientation: inline

hGH
type: SequenceAnnotation
persistent identity: annotation2093297
display id: annotation2093297
version: 1
top level: BBa_K404126
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404126/annotation2093297/range2093297/1

https://synbiohub.org/public/igem/BBa_K404126/annotation2093297/range2093297/1
type: Range
persistent identity: range2093297
display id: range2093297
version: 1
direction: direction/forward
top level: BBa_K404126
start: 2623
end: 3101
orientation: inline

right ITR
type: SequenceAnnotation
persistent identity: annotation2093296
display id: annotation2093296
version: 1
top level: BBa_K404126
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404126/annotation2093296/range2093296/1

https://synbiohub.org/public/igem/BBa_K404126/annotation2093296/range2093296/1
type: Range
persistent identity: range2093296
display id: range2093296
version: 1
direction: direction/forward
top level: BBa_K404126
start: 3116
end: 3253
orientation: inline

left ITR
type: SequenceAnnotation
persistent identity: annotation2093290
display id: annotation2093290
version: 1
top level: BBa_K404126
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404126/annotation2093290/range2093290/1

https://synbiohub.org/public/igem/BBa_K404126/annotation2093290/range2093290/1
type: Range
persistent identity: range2093290
display id: range2093290
version: 1
direction: direction/forward
top level: BBa_K404126
start: 1
end: 147
orientation: inline

CMV promoter
type: SequenceAnnotation
persistent identity: annotation2093292
display id: annotation2093292
version: 1
top level: BBa_K404126
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K404126/annotation2093292/range2093292/1

https://synbiohub.org/public/igem/BBa_K404126/annotation2093292/range2093292/1
type: Range
persistent identity: range2093292
display id: range2093292
version: 1
direction: direction/forward
top level: BBa_K404126
start: 155
end: 809
orientation: inline

BBa_I712017
type: ComponentDefinition
persistent identity: BBa I712017
display id: BBa_I712017
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Chemokine (CXC motif) receptor 4, fused to N-terminal half of ubiquitin.
creator: Anja Korenčič
created: 2007-10-20T11:00:00Z
modified: 2015-08-31T04:07:46Z
discontinued: false
dominant: false
group u list: _130_
m user id: 0
owner id: 1846
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Chemokine (CXC motif) receptor 4, fused to N-terminal half of ubiquitin.
mutable notes: CXCR4 and NUb fragments were fused with PCR overlap extension method without any linker added. The part is cloned into BBa_J52017 (a vector with eukaryontic terminator).
mutable provenance: CXCR4 gene obtained from Origene, NUb gene obtained from Dualsystems.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: NUb
sequence annotation: CXCR4
sequence: https://synbiohub.org/public/igem/BBa_I712017_sequence/1

NUb
type: SequenceAnnotation
persistent identity: annotation1951575
display id: annotation1951575
version: 1
top level: BBa_I712017
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_I712017/annotation1951575/range1951575/1

https://synbiohub.org/public/igem/BBa_I712017/annotation1951575/range1951575/1
type: Range
persistent identity: range1951575
display id: range1951575
version: 1
direction: direction/forward
top level: BBa_I712017
start: 1057
end: 1191
orientation: inline

CXCR4
type: SequenceAnnotation
persistent identity: annotation1951574
display id: annotation1951574
version: 1
top level: BBa_I712017
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_I712017/annotation1951574/range1951574/1

https://synbiohub.org/public/igem/BBa_I712017/annotation1951574/range1951574/1
type: Range
persistent identity: range1951574
display id: range1951574
version: 1
direction: direction/forward
top level: BBa_I712017
start: 1
end: 1056
orientation: inline

BBa_K404006
type: ComponentDefinition
persistent identity: BBa K404006
display id: BBa_K404006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: [AAV2]-VP123
creator: Freiburg Bioware 2010
created: 2010-10-20T11:00:00Z
modified: 2015-05-08T01:12:23Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 6674
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: VP3
sequence annotation: VP2
sequence annotation: VP1
sequence: https://synbiohub.org/public/igem/BBa_K404006_sequence/1

VP3
type: SequenceAnnotation
persistent identity: annotation2092162
display id: annotation2092162
version: 1
top level: BBa_K404006
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404006/annotation2092162/range2092162/1

https://synbiohub.org/public/igem/BBa_K404006/annotation2092162/range2092162/1
type: Range
persistent identity: range2092162
display id: range2092162
version: 1
direction: direction/forward
top level: BBa_K404006
start: 631
end: 2208
orientation: inline

VP2
type: SequenceAnnotation
persistent identity: annotation2092161
display id: annotation2092161
version: 1
top level: BBa_K404006
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404006/annotation2092161/range2092161/1

https://synbiohub.org/public/igem/BBa_K404006/annotation2092161/range2092161/1
type: Range
persistent identity: range2092161
display id: range2092161
version: 1
direction: direction/forward
top level: BBa_K404006
start: 412
end: 2208
orientation: inline

VP1
type: SequenceAnnotation
persistent identity: annotation2092160
display id: annotation2092160
version: 1
top level: BBa_K404006
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404006/annotation2092160/range2092160/1

https://synbiohub.org/public/igem/BBa_K404006/annotation2092160/range2092160/1
type: Range
persistent identity: range2092160
display id: range2092160
version: 1
direction: direction/forward
top level: BBa_K404006
start: 1
end: 2208
orientation: inline

BBa_K404013
type: ComponentDefinition
persistent identity: BBa K404013
display id: BBa_K404013
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pCMV_[AAV2]-VP3
creator: Freiburg Bioware 2010
created: 2010-10-20T11:00:00Z
modified: 2015-05-08T01:12:23Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 6674
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: CMV
sequence annotation: VP3
sequence: https://synbiohub.org/public/igem/BBa_K404013_sequence/1

CMV
type: SequenceAnnotation
persistent identity: annotation2097906
display id: annotation2097906
version: 1
top level: BBa_K404013
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K404013/annotation2097906/range2097906/1

https://synbiohub.org/public/igem/BBa_K404013/annotation2097906/range2097906/1
type: Range
persistent identity: range2097906
display id: range2097906
version: 1
direction: direction/forward
top level: BBa_K404013
start: 2
end: 655
orientation: inline

VP3
type: SequenceAnnotation
persistent identity: annotation2097907
display id: annotation2097907
version: 1
top level: BBa_K404013
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404013/annotation2097907/range2097907/1

https://synbiohub.org/public/igem/BBa_K404013/annotation2097907/range2097907/1
type: Range
persistent identity: range2097907
display id: range2097907
version: 1
direction: direction/forward
top level: BBa_K404013
start: 670
end: 2305
orientation: inline

eGFP
type: ComponentDefinition
persistent identity: BBa K678006
display id: BBa_K678006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: eGFP for mammalian cells
creator: DTU-Denmark-2
created: 2011-09-03T11:00:00Z
modified: 2015-05-08T01:13:04Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _882_
m user id: 0
owner id: 8341
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: enhanced green fluorescent protein for use in mammalian cells
mutable notes: Compatible with the Plug'n'Play assembly standard
mutable provenance: plasmid pEGFP-1 from Clontech
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: eGFP
sequence annotation: Kozak
sequence annotation: Linker 2
sequence: https://synbiohub.org/public/igem/BBa_K678006_sequence/1

eGFP
type: SequenceAnnotation
persistent identity: annotation2127349
display id: annotation2127349
version: 1
top level: eGFP
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K678006/annotation2127349/range2127349/1

https://synbiohub.org/public/igem/BBa_K678006/annotation2127349/range2127349/1
type: Range
persistent identity: range2127349
display id: range2127349
version: 1
direction: direction/forward
top level: eGFP
start: 16
end: 735
orientation: inline

Kozak
type: SequenceAnnotation
persistent identity: annotation2127774
display id: annotation2127774
version: 1
top level: eGFP
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K678006/annotation2127774/range2127774/1

https://synbiohub.org/public/igem/BBa_K678006/annotation2127774/range2127774/1
type: Range
persistent identity: range2127774
display id: range2127774
version: 1
direction: direction/forward
top level: eGFP
start: 9
end: 19
orientation: inline

Linker 2
type: SequenceAnnotation
persistent identity: annotation2127348
display id: annotation2127348
version: 1
top level: eGFP
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K678006/annotation2127348/range2127348/1

https://synbiohub.org/public/igem/BBa_K678006/annotation2127348/range2127348/1
type: Range
persistent identity: range2127348
display id: range2127348
version: 1
direction: direction/forward
top level: eGFP
start: 1
end: 8
orientation: inline

hGH pA
type: ComponentDefinition
persistent identity: BBa K678018
display id: BBa_K678018
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: hGH poly A, terminator for mammalian cells
creator: DTU-Denmark-2
created: 2011-09-03T11:00:00Z
modified: 2015-05-08T01:13:04Z
discontinued: false
dominant: false
experience: experience/None
group u list: _882_
m user id: 0
owner id: 8341
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: hGH poly A, terminator for mammalian cells
mutable notes: Compatible with the Plug'n'Play assembly standard
mutable provenance: From plasmid N-terminal pFLAG-CMV from Sigma-Aldrich
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: hGH
sequence annotation: Linker 4
sequence: https://synbiohub.org/public/igem/BBa_K678018_sequence/1

hGH
type: SequenceAnnotation
persistent identity: annotation2127351
display id: annotation2127351
version: 1
top level: hGH pA
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_K678018/annotation2127351/range2127351/1

https://synbiohub.org/public/igem/BBa_K678018/annotation2127351/range2127351/1
type: Range
persistent identity: range2127351
display id: range2127351
version: 1
direction: direction/forward
top level: hGH pA
start: 9
end: 635
orientation: inline

Linker 4
type: SequenceAnnotation
persistent identity: annotation2127350
display id: annotation2127350
version: 1
top level: hGH pA
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K678018/annotation2127350/range2127350/1

https://synbiohub.org/public/igem/BBa_K678018/annotation2127350/range2127350/1
type: Range
persistent identity: range2127350
display id: range2127350
version: 1
direction: direction/forward
top level: hGH pA
start: 1
end: 8
orientation: inline

BBa_K1777005
type: ComponentDefinition
persistent identity: BBa K1777005
display id: BBa_K1777005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: flexible linker with SV40 NLS
creator: Xuhang Li
created: 2015-09-12T11:00:00Z
modified: 2015-09-19T11:42:57Z
discontinued: false
dominant: false
experience: experience/Issues
group u list: _2199_
m user id: 27798
owner id: 25645
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This is a 40-50A flexible separation for fusion domains with a SV40 NLS, and its codon is optimized for mammalian. It can be used to link different protein domains for fusion proteins locating in cell nucleus.
mutable notes: The codon is optimized for mammalian, and we insert a NLS to promote locating in cell nucleus. Besides ,we lengthen this linker to provide better separation of two domains. At last we avoid tandem repeat sequence(like GGGSPKKKRKVGGGS), which will facilitate fusing this linker to your domains via overlap PCR.
mutable provenance: This part is improved from BBa_K1486003.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: SV40 NLS
sequence annotation: artificial GS linker
sequence annotation: scar from 3A assembly
sequence annotation: natural flexible linker
sequence annotation: the first linker
sequence annotation: the second linker
sequence: https://synbiohub.org/public/igem/BBa_K1777005_sequence/1

SV40 NLS
type: SequenceAnnotation
persistent identity: annotation2476142
display id: annotation2476142
version: 1
top level: BBa_K1777005
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1777005/annotation2476142/range2476142/1

https://synbiohub.org/public/igem/BBa_K1777005/annotation2476142/range2476142/1
type: Range
persistent identity: range2476142
display id: range2476142
version: 1
direction: direction/forward
top level: BBa_K1777005
start: 24
end: 44
orientation: inline

artificial GS linker
type: SequenceAnnotation
persistent identity: annotation2476145
display id: annotation2476145
version: 1
top level: BBa_K1777005
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1777005/annotation2476145/range2476145/1

https://synbiohub.org/public/igem/BBa_K1777005/annotation2476145/range2476145/1
type: Range
persistent identity: range2476145
display id: range2476145
version: 1
direction: direction/forward
top level: BBa_K1777005
start: 45
end: 59
orientation: inline

artificial GS linker
type: SequenceAnnotation
persistent identity: annotation2476147
display id: annotation2476147
version: 1
top level: BBa_K1777005
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1777005/annotation2476147/range2476147/1

https://synbiohub.org/public/igem/BBa_K1777005/annotation2476147/range2476147/1
type: Range
persistent identity: range2476147
display id: range2476147
version: 1
direction: direction/forward
top level: BBa_K1777005
start: 110
end: 124
orientation: inline

SV40 NLS
type: SequenceAnnotation
persistent identity: annotation2476143
display id: annotation2476143
version: 1
top level: BBa_K1777005
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1777005/annotation2476143/range2476143/1

https://synbiohub.org/public/igem/BBa_K1777005/annotation2476143/range2476143/1
type: Range
persistent identity: range2476143
display id: range2476143
version: 1
direction: direction/forward
top level: BBa_K1777005
start: 89
end: 109
orientation: inline

scar from 3A assembly
type: SequenceAnnotation
persistent identity: annotation2476141
display id: annotation2476141
version: 1
top level: BBa_K1777005
role: feature/scar
location: https://synbiohub.org/public/igem/BBa_K1777005/annotation2476141/range2476141/1

https://synbiohub.org/public/igem/BBa_K1777005/annotation2476141/range2476141/1
type: Range
persistent identity: range2476141
display id: range2476141
version: 1
direction: direction/forward
top level: BBa_K1777005
start: 60
end: 67
orientation: inline

natural flexible linker
type: SequenceAnnotation
persistent identity: annotation2476146
display id: annotation2476146
version: 1
top level: BBa_K1777005
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1777005/annotation2476146/range2476146/1

https://synbiohub.org/public/igem/BBa_K1777005/annotation2476146/range2476146/1
type: Range
persistent identity: range2476146
display id: range2476146
version: 1
direction: direction/forward
top level: BBa_K1777005
start: 68
end: 88
orientation: inline

natural flexible linker
type: SequenceAnnotation
persistent identity: annotation2476144
display id: annotation2476144
version: 1
top level: BBa_K1777005
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1777005/annotation2476144/range2476144/1

https://synbiohub.org/public/igem/BBa_K1777005/annotation2476144/range2476144/1
type: Range
persistent identity: range2476144
display id: range2476144
version: 1
direction: direction/forward
top level: BBa_K1777005
start: 3
end: 23
orientation: inline

the first linker
type: SequenceAnnotation
persistent identity: annotation2476139
display id: annotation2476139
version: 1
top level: BBa_K1777005
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1777005/annotation2476139/range2476139/1

https://synbiohub.org/public/igem/BBa_K1777005/annotation2476139/range2476139/1
type: Range
persistent identity: range2476139
display id: range2476139
version: 1
direction: direction/forward
top level: BBa_K1777005
start: 3
end: 59
orientation: inline

the second linker
type: SequenceAnnotation
persistent identity: annotation2476140
display id: annotation2476140
version: 1
top level: BBa_K1777005
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1777005/annotation2476140/range2476140/1

https://synbiohub.org/public/igem/BBa_K1777005/annotation2476140/range2476140/1
type: Range
persistent identity: range2476140
display id: range2476140
version: 1
direction: direction/forward
top level: BBa_K1777005
start: 68
end: 124
orientation: inline

BBa_K404090
type: ComponentDefinition
persistent identity: BBa K404090
display id: BBa_K404090
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: [AAV2]-Rep40ex
creator: Freiburg Bioware 2010
created: 2010-08-27T11:00:00Z
modified: 2015-05-08T01:12:23Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 7049
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: Rep40
sequence: https://synbiohub.org/public/igem/BBa_K404090_sequence/1

Rep40
type: SequenceAnnotation
persistent identity: annotation2093160
display id: annotation2093160
version: 1
top level: BBa_K404090
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404090/annotation2093160/range2093160/1

https://synbiohub.org/public/igem/BBa_K404090/annotation2093160/range2093160/1
type: Range
persistent identity: range2093160
display id: range2093160
version: 1
direction: direction/forward
top level: BBa_K404090
start: 1
end: 940
orientation: inline

BBa_K404004
type: ComponentDefinition
persistent identity: BBa K404004
display id: BBa_K404004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: [AAV2]-Rep-VP13(ViralBrick-587KO-empty)_p5-TATAless
creator: Freiburg Bioware 2010
created: 2010-10-20T11:00:00Z
modified: 2015-05-08T01:12:23Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 6674
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: basic region 4
sequence annotation: HSPG-KO
sequence annotation: p40
sequence annotation: VP1
sequence annotation: G--> C VP2 KO
sequence annotation: PLA2
sequence annotation: scar
sequence annotation: AAV-2-cap (Stratagene)
sequence annotation: p5TATAless
sequence annotation: Rep52
sequence annotation: Rep68
sequence annotation: Rep78
sequence annotation: p19
sequence annotation: VP3
sequence annotation: basic region 2
sequence annotation: basic region 1
sequence annotation: Rep40
sequence annotation: basic region 3
sequence annotation: HSPK-KO
sequence: https://synbiohub.org/public/igem/BBa_K404004_sequence/1

basic region 4
type: SequenceAnnotation
persistent identity: annotation2099622
display id: annotation2099622
version: 1
top level: BBa_K404004
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404004/annotation2099622/range2099622/1

https://synbiohub.org/public/igem/BBa_K404004/annotation2099622/range2099622/1
type: Range
persistent identity: range2099622
display id: range2099622
version: 1
direction: direction/forward
top level: BBa_K404004
start: 2801
end: 2818
orientation: inline

HSPG-KO
type: SequenceAnnotation
persistent identity: annotation2099626
display id: annotation2099626
version: 1
top level: BBa_K404004
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K404004/annotation2099626/range2099626/1

https://synbiohub.org/public/igem/BBa_K404004/annotation2099626/range2099626/1
type: Range
persistent identity: range2099626
display id: range2099626
version: 1
direction: direction/forward
top level: BBa_K404004
start: 3635
end: 3637
orientation: inline

p40
type: SequenceAnnotation
persistent identity: annotation2099614
display id: annotation2099614
version: 1
top level: BBa_K404004
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K404004/annotation2099614/range2099614/1

https://synbiohub.org/public/igem/BBa_K404004/annotation2099614/range2099614/1
type: Range
persistent identity: range2099614
display id: range2099614
version: 1
direction: direction/forward
top level: BBa_K404004
start: 1390
end: 1507
orientation: inline

VP1
type: SequenceAnnotation
persistent identity: annotation2099616
display id: annotation2099616
version: 1
top level: BBa_K404004
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K404004/annotation2099616/range2099616/1

https://synbiohub.org/public/igem/BBa_K404004/annotation2099616/range2099616/1
type: Range
persistent identity: range2099616
display id: range2099616
version: 1
direction: direction/forward
top level: BBa_K404004
start: 1883
end: 4090
orientation: inline

G--> C VP2 KO
type: SequenceAnnotation
persistent identity: annotation2099624
display id: annotation2099624
version: 1
top level: BBa_K404004
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K404004/annotation2099624/range2099624/1

https://synbiohub.org/public/igem/BBa_K404004/annotation2099624/range2099624/1
type: Range
persistent identity: range2099624
display id: range2099624
version: 1
direction: direction/forward
top level: BBa_K404004
start: 2296
end: 2296
orientation: inline

PLA2
type: SequenceAnnotation
persistent identity: annotation2099617
display id: annotation2099617
version: 1
top level: BBa_K404004
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404004/annotation2099617/range2099617/1

https://synbiohub.org/public/igem/BBa_K404004/annotation2099617/range2099617/1
type: Range
persistent identity: range2099617
display id: range2099617
version: 1
direction: direction/forward
top level: BBa_K404004
start: 2015
end: 2191
orientation: inline

scar
type: SequenceAnnotation
persistent identity: annotation2101675
display id: annotation2101675
version: 1
top level: BBa_K404004
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404004/annotation2101675/range2101675/1

https://synbiohub.org/public/igem/BBa_K404004/annotation2101675/range2101675/1
type: Range
persistent identity: range2101675
display id: range2101675
version: 1
direction: direction/forward
top level: BBa_K404004
start: 4217
end: 4327
orientation: inline

AAV-2-cap (Stratagene)
type: SequenceAnnotation
persistent identity: annotation2099615
display id: annotation2099615
version: 1
top level: BBa_K404004
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K404004/annotation2099615/range2099615/1

https://synbiohub.org/public/igem/BBa_K404004/annotation2099615/range2099615/1
type: Range
persistent identity: range2099615
display id: range2099615
version: 1
direction: direction/forward
top level: BBa_K404004
start: 1883
end: 4216
orientation: inline

p5TATAless
type: SequenceAnnotation
persistent identity: annotation2099623
display id: annotation2099623
version: 1
top level: BBa_K404004
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K404004/annotation2099623/range2099623/1

https://synbiohub.org/public/igem/BBa_K404004/annotation2099623/range2099623/1
type: Range
persistent identity: range2099623
display id: range2099623
version: 1
direction: direction/forward
top level: BBa_K404004
start: 4328
end: 4401
orientation: inline

Rep52
type: SequenceAnnotation
persistent identity: annotation2099613
display id: annotation2099613
version: 1
top level: BBa_K404004
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K404004/annotation2099613/range2099613/1

https://synbiohub.org/public/igem/BBa_K404004/annotation2099613/range2099613/1
type: Range
persistent identity: range2099613
display id: range2099613
version: 1
direction: direction/forward
top level: BBa_K404004
start: 673
end: 1867
orientation: inline

Rep68
type: SequenceAnnotation
persistent identity: annotation2099609
display id: annotation2099609
version: 1
top level: BBa_K404004
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K404004/annotation2099609/range2099609/1

https://synbiohub.org/public/igem/BBa_K404004/annotation2099609/range2099609/1
type: Range
persistent identity: range2099609
display id: range2099609
version: 1
direction: direction/forward
top level: BBa_K404004
start: 1
end: 1933
orientation: inline

Rep78
type: SequenceAnnotation
persistent identity: annotation2099610
display id: annotation2099610
version: 1
top level: BBa_K404004
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K404004/annotation2099610/range2099610/1

https://synbiohub.org/public/igem/BBa_K404004/annotation2099610/range2099610/1
type: Range
persistent identity: range2099610
display id: range2099610
version: 1
direction: direction/forward
top level: BBa_K404004
start: 1
end: 1867
orientation: inline

p19
type: SequenceAnnotation
persistent identity: annotation2099611
display id: annotation2099611
version: 1
top level: BBa_K404004
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K404004/annotation2099611/range2099611/1

https://synbiohub.org/public/igem/BBa_K404004/annotation2099611/range2099611/1
type: Range
persistent identity: range2099611
display id: range2099611
version: 1
direction: direction/forward
top level: BBa_K404004
start: 410
end: 529
orientation: inline

VP3
type: SequenceAnnotation
persistent identity: annotation2099621
display id: annotation2099621
version: 1
top level: BBa_K404004
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K404004/annotation2099621/range2099621/1

https://synbiohub.org/public/igem/BBa_K404004/annotation2099621/range2099621/1
type: Range
persistent identity: range2099621
display id: range2099621
version: 1
direction: direction/forward
top level: BBa_K404004
start: 2489
end: 4090
orientation: inline

basic region 2
type: SequenceAnnotation
persistent identity: annotation2099619
display id: annotation2099619
version: 1
top level: BBa_K404004
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404004/annotation2099619/range2099619/1

https://synbiohub.org/public/igem/BBa_K404004/annotation2099619/range2099619/1
type: Range
persistent identity: range2099619
display id: range2099619
version: 1
direction: direction/forward
top level: BBa_K404004
start: 2300
end: 2320
orientation: inline

basic region 1
type: SequenceAnnotation
persistent identity: annotation2099618
display id: annotation2099618
version: 1
top level: BBa_K404004
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404004/annotation2099618/range2099618/1

https://synbiohub.org/public/igem/BBa_K404004/annotation2099618/range2099618/1
type: Range
persistent identity: range2099618
display id: range2099618
version: 1
direction: direction/forward
top level: BBa_K404004
start: 2240
end: 2260
orientation: inline

Rep40
type: SequenceAnnotation
persistent identity: annotation2099612
display id: annotation2099612
version: 1
top level: BBa_K404004
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K404004/annotation2099612/range2099612/1

https://synbiohub.org/public/igem/BBa_K404004/annotation2099612/range2099612/1
type: Range
persistent identity: range2099612
display id: range2099612
version: 1
direction: direction/forward
top level: BBa_K404004
start: 673
end: 1933
orientation: inline

basic region 3
type: SequenceAnnotation
persistent identity: annotation2099620
display id: annotation2099620
version: 1
top level: BBa_K404004
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404004/annotation2099620/range2099620/1

https://synbiohub.org/public/igem/BBa_K404004/annotation2099620/range2099620/1
type: Range
persistent identity: range2099620
display id: range2099620
version: 1
direction: direction/forward
top level: BBa_K404004
start: 2378
end: 2398
orientation: inline

HSPK-KO
type: SequenceAnnotation
persistent identity: annotation2099625
display id: annotation2099625
version: 1
top level: BBa_K404004
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K404004/annotation2099625/range2099625/1

https://synbiohub.org/public/igem/BBa_K404004/annotation2099625/range2099625/1
type: Range
persistent identity: range2099625
display id: range2099625
version: 1
direction: direction/forward
top level: BBa_K404004
start: 3644
end: 3646
orientation: inline

BBa_K404105
type: ComponentDefinition
persistent identity: BBa K404105
display id: BBa_K404105
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: p40 promoter
creator: Freiburg Bioware 2010
created: 2010-08-27T11:00:00Z
modified: 2015-05-08T01:12:23Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 7049
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: p40 promoter
sequence: https://synbiohub.org/public/igem/BBa_K404105_sequence/1

p40 promoter
type: SequenceAnnotation
persistent identity: annotation2093170
display id: annotation2093170
version: 1
top level: BBa_K404105
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K404105/annotation2093170/range2093170/1

https://synbiohub.org/public/igem/BBa_K404105/annotation2093170/range2093170/1
type: Range
persistent identity: range2093170
display id: range2093170
version: 1
direction: direction/forward
top level: BBa_K404105
start: 1
end: 182
orientation: inline

BBa_K404203
type: ComponentDefinition
persistent identity: BBa K404203
display id: BBa_K404203
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: ViralBrick-453-RGD
creator: Freiburg Bioware 2010
created: 2010-08-27T11:00:00Z
modified: 2015-05-08T01:12:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 7049
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: upstream 453
sequence annotation: A Spacer
sequence annotation: RGD
sequence annotation: downstream 453
sequence: https://synbiohub.org/public/igem/BBa_K404203_sequence/1

upstream 453
type: SequenceAnnotation
persistent identity: annotation2078939
display id: annotation2078939
version: 1
top level: BBa_K404203
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404203/annotation2078939/range2078939/1

https://synbiohub.org/public/igem/BBa_K404203/annotation2078939/range2078939/1
type: Range
persistent identity: range2078939
display id: range2078939
version: 1
direction: direction/forward
top level: BBa_K404203
start: 1
end: 42
orientation: inline

A Spacer
type: SequenceAnnotation
persistent identity: annotation2078942
display id: annotation2078942
version: 1
top level: BBa_K404203
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404203/annotation2078942/range2078942/1

https://synbiohub.org/public/igem/BBa_K404203/annotation2078942/range2078942/1
type: Range
persistent identity: range2078942
display id: range2078942
version: 1
direction: direction/forward
top level: BBa_K404203
start: 73
end: 75
orientation: inline

RGD
type: SequenceAnnotation
persistent identity: annotation2078941
display id: annotation2078941
version: 1
top level: BBa_K404203
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404203/annotation2078941/range2078941/1

https://synbiohub.org/public/igem/BBa_K404203/annotation2078941/range2078941/1
type: Range
persistent identity: range2078941
display id: range2078941
version: 1
direction: direction/forward
top level: BBa_K404203
start: 46
end: 72
orientation: inline

A Spacer
type: SequenceAnnotation
persistent identity: annotation2078940
display id: annotation2078940
version: 1
top level: BBa_K404203
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404203/annotation2078940/range2078940/1

https://synbiohub.org/public/igem/BBa_K404203/annotation2078940/range2078940/1
type: Range
persistent identity: range2078940
display id: range2078940
version: 1
direction: direction/forward
top level: BBa_K404203
start: 43
end: 45
orientation: inline

downstream 453
type: SequenceAnnotation
persistent identity: annotation2078943
display id: annotation2078943
version: 1
top level: BBa_K404203
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404203/annotation2078943/range2078943/1

https://synbiohub.org/public/igem/BBa_K404203/annotation2078943/range2078943/1
type: Range
persistent identity: range2078943
display id: range2078943
version: 1
direction: direction/forward
top level: BBa_K404203
start: 76
end: 96
orientation: inline

BBa_K404164
type: ComponentDefinition
persistent identity: BBa K404164
display id: BBa_K404164
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pCMV_VP1up_NLS_Z-EGFR-1907_[AAV2]-VP23 (ViralBrick-587KO-Empty)
creator: Freiburg Bioware 2010
created: 2010-10-10T11:00:00Z
modified: 2015-05-08T01:12:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 6733
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: CMV
sequence annotation: HSPG-KO
sequence annotation: VP1up
sequence annotation: AAV-2-cap (Stratagene)
sequence annotation: NLS
sequence annotation: VP3
sequence annotation: VP2
sequence annotation: ZEGFR_1907
sequence: https://synbiohub.org/public/igem/BBa_K404164_sequence/1

CMV
type: SequenceAnnotation
persistent identity: annotation2095191
display id: annotation2095191
version: 1
top level: BBa_K404164
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K404164/annotation2095191/range2095191/1

https://synbiohub.org/public/igem/BBa_K404164/annotation2095191/range2095191/1
type: Range
persistent identity: range2095191
display id: range2095191
version: 1
direction: direction/forward
top level: BBa_K404164
start: 2
end: 655
orientation: inline

HSPG-KO
type: SequenceAnnotation
persistent identity: annotation2101815
display id: annotation2101815
version: 1
top level: BBa_K404164
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K404164/annotation2101815/range2101815/1

https://synbiohub.org/public/igem/BBa_K404164/annotation2101815/range2101815/1
type: Range
persistent identity: range2101815
display id: range2101815
version: 1
direction: direction/forward
top level: BBa_K404164
start: 2633
end: 2635
orientation: inline

VP1up
type: SequenceAnnotation
persistent identity: annotation2095192
display id: annotation2095192
version: 1
top level: BBa_K404164
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404164/annotation2095192/range2095192/1

https://synbiohub.org/public/igem/BBa_K404164/annotation2095192/range2095192/1
type: Range
persistent identity: range2095192
display id: range2095192
version: 1
direction: direction/forward
top level: BBa_K404164
start: 673
end: 1081
orientation: inline

AAV-2-cap (Stratagene)
type: SequenceAnnotation
persistent identity: annotation2095199
display id: annotation2095199
version: 1
top level: BBa_K404164
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404164/annotation2095199/range2095199/1

https://synbiohub.org/public/igem/BBa_K404164/annotation2095199/range2095199/1
type: Range
persistent identity: range2095199
display id: range2095199
version: 1
direction: direction/forward
top level: BBa_K404164
start: 1295
end: 3211
orientation: inline

NLS
type: SequenceAnnotation
persistent identity: annotation2095197
display id: annotation2095197
version: 1
top level: BBa_K404164
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404164/annotation2095197/range2095197/1

https://synbiohub.org/public/igem/BBa_K404164/annotation2095197/range2095197/1
type: Range
persistent identity: range2095197
display id: range2095197
version: 1
direction: direction/forward
top level: BBa_K404164
start: 1088
end: 1108
orientation: inline

VP3
type: SequenceAnnotation
persistent identity: annotation2095195
display id: annotation2095195
version: 1
top level: BBa_K404164
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404164/annotation2095195/range2095195/1

https://synbiohub.org/public/igem/BBa_K404164/annotation2095195/range2095195/1
type: Range
persistent identity: range2095195
display id: range2095195
version: 1
direction: direction/forward
top level: BBa_K404164
start: 1487
end: 3088
orientation: inline

VP2
type: SequenceAnnotation
persistent identity: annotation2095194
display id: annotation2095194
version: 1
top level: BBa_K404164
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404164/annotation2095194/range2095194/1

https://synbiohub.org/public/igem/BBa_K404164/annotation2095194/range2095194/1
type: Range
persistent identity: range2095194
display id: range2095194
version: 1
direction: direction/forward
top level: BBa_K404164
start: 1295
end: 3088
orientation: inline

HSPG-KO
type: SequenceAnnotation
persistent identity: annotation2101814
display id: annotation2101814
version: 1
top level: BBa_K404164
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K404164/annotation2101814/range2101814/1

https://synbiohub.org/public/igem/BBa_K404164/annotation2101814/range2101814/1
type: Range
persistent identity: range2101814
display id: range2101814
version: 1
direction: direction/forward
top level: BBa_K404164
start: 2642
end: 2644
orientation: inline

ZEGFR_1907
type: SequenceAnnotation
persistent identity: annotation2095193
display id: annotation2095193
version: 1
top level: BBa_K404164
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404164/annotation2095193/range2095193/1

https://synbiohub.org/public/igem/BBa_K404164/annotation2095193/range2095193/1
type: Range
persistent identity: range2095193
display id: range2095193
version: 1
direction: direction/forward
top level: BBa_K404164
start: 1115
end: 1228
orientation: inline

BBa_K404222
type: ComponentDefinition
persistent identity: BBa K404222
display id: BBa_K404222
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: [AAV2]-Rep-VP123_P5-TATAless (ViralBrick-453-His-Tag)
creator: Freiburg Bioware 2010
created: 2010-08-27T11:00:00Z
modified: 2015-05-08T01:12:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 7049
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: VP2
sequence annotation: A-spacer
sequence annotation: Rep52
sequence annotation: VP3
sequence annotation: VP1
sequence annotation: His-tag
sequence annotation: Rep68
sequence annotation: p5TATAless
sequence annotation: Rep78
sequence annotation: AAV-a-cap (Stratagene)
sequence annotation: Rep40
sequence: https://synbiohub.org/public/igem/BBa_K404222_sequence/1

VP2
type: SequenceAnnotation
persistent identity: annotation2098256
display id: annotation2098256
version: 1
top level: BBa_K404222
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404222/annotation2098256/range2098256/1

https://synbiohub.org/public/igem/BBa_K404222/annotation2098256/range2098256/1
type: Range
persistent identity: range2098256
display id: range2098256
version: 1
direction: direction/forward
top level: BBa_K404222
start: 2294
end: 4114
orientation: inline

A-spacer
type: SequenceAnnotation
persistent identity: annotation2098262
display id: annotation2098262
version: 1
top level: BBa_K404222
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404222/annotation2098262/range2098262/1

https://synbiohub.org/public/igem/BBa_K404222/annotation2098262/range2098262/1
type: Range
persistent identity: range2098262
display id: range2098262
version: 1
direction: direction/forward
top level: BBa_K404222
start: 3263
end: 3265
orientation: inline

Rep52
type: SequenceAnnotation
persistent identity: annotation2098254
display id: annotation2098254
version: 1
top level: BBa_K404222
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404222/annotation2098254/range2098254/1

https://synbiohub.org/public/igem/BBa_K404222/annotation2098254/range2098254/1
type: Range
persistent identity: range2098254
display id: range2098254
version: 1
direction: direction/forward
top level: BBa_K404222
start: 673
end: 1867
orientation: inline

VP3
type: SequenceAnnotation
persistent identity: annotation2098257
display id: annotation2098257
version: 1
top level: BBa_K404222
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404222/annotation2098257/range2098257/1

https://synbiohub.org/public/igem/BBa_K404222/annotation2098257/range2098257/1
type: Range
persistent identity: range2098257
display id: range2098257
version: 1
direction: direction/forward
top level: BBa_K404222
start: 2489
end: 4114
orientation: inline

VP1
type: SequenceAnnotation
persistent identity: annotation2098255
display id: annotation2098255
version: 1
top level: BBa_K404222
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404222/annotation2098255/range2098255/1

https://synbiohub.org/public/igem/BBa_K404222/annotation2098255/range2098255/1
type: Range
persistent identity: range2098255
display id: range2098255
version: 1
direction: direction/forward
top level: BBa_K404222
start: 1883
end: 4114
orientation: inline

His-tag
type: SequenceAnnotation
persistent identity: annotation2098260
display id: annotation2098260
version: 1
top level: BBa_K404222
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404222/annotation2098260/range2098260/1

https://synbiohub.org/public/igem/BBa_K404222/annotation2098260/range2098260/1
type: Range
persistent identity: range2098260
display id: range2098260
version: 1
direction: direction/forward
top level: BBa_K404222
start: 3245
end: 3262
orientation: inline

A-spacer
type: SequenceAnnotation
persistent identity: annotation2098261
display id: annotation2098261
version: 1
top level: BBa_K404222
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404222/annotation2098261/range2098261/1

https://synbiohub.org/public/igem/BBa_K404222/annotation2098261/range2098261/1
type: Range
persistent identity: range2098261
display id: range2098261
version: 1
direction: direction/forward
top level: BBa_K404222
start: 3242
end: 3244
orientation: inline

Rep68
type: SequenceAnnotation
persistent identity: annotation2098252
display id: annotation2098252
version: 1
top level: BBa_K404222
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404222/annotation2098252/range2098252/1

https://synbiohub.org/public/igem/BBa_K404222/annotation2098252/range2098252/1
type: Range
persistent identity: range2098252
display id: range2098252
version: 1
direction: direction/forward
top level: BBa_K404222
start: 2
end: 1933
orientation: inline

p5TATAless
type: SequenceAnnotation
persistent identity: annotation2098258
display id: annotation2098258
version: 1
top level: BBa_K404222
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404222/annotation2098258/range2098258/1

https://synbiohub.org/public/igem/BBa_K404222/annotation2098258/range2098258/1
type: Range
persistent identity: range2098258
display id: range2098258
version: 1
direction: direction/forward
top level: BBa_K404222
start: 4246
end: 4409
orientation: inline

Rep78
type: SequenceAnnotation
persistent identity: annotation2098251
display id: annotation2098251
version: 1
top level: BBa_K404222
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404222/annotation2098251/range2098251/1

https://synbiohub.org/public/igem/BBa_K404222/annotation2098251/range2098251/1
type: Range
persistent identity: range2098251
display id: range2098251
version: 1
direction: direction/forward
top level: BBa_K404222
start: 2
end: 1867
orientation: inline

AAV-a-cap (Stratagene)
type: SequenceAnnotation
persistent identity: annotation2098259
display id: annotation2098259
version: 1
top level: BBa_K404222
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404222/annotation2098259/range2098259/1

https://synbiohub.org/public/igem/BBa_K404222/annotation2098259/range2098259/1
type: Range
persistent identity: range2098259
display id: range2098259
version: 1
direction: direction/forward
top level: BBa_K404222
start: 1883
end: 4237
orientation: inline

Rep40
type: SequenceAnnotation
persistent identity: annotation2098253
display id: annotation2098253
version: 1
top level: BBa_K404222
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404222/annotation2098253/range2098253/1

https://synbiohub.org/public/igem/BBa_K404222/annotation2098253/range2098253/1
type: Range
persistent identity: range2098253
display id: range2098253
version: 1
direction: direction/forward
top level: BBa_K404222
start: 673
end: 1933
orientation: inline

BBa_I712010
type: ComponentDefinition
persistent identity: BBa I712010
display id: BBa_I712010
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CD4 sequence without signal peptide
creator: peter cimermancic
created: 2007-10-22T11:00:00Z
modified: 2015-08-31T04:07:45Z
discontinued: false
dominant: false
group u list: _130_
m user id: 0
owner id: 1850
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: It`s a chemokine receptor protein.
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_I712010_sequence/1

BBa_K1720004
type: ComponentDefinition
persistent identity: BBa K1720004
display id: BBa_K1720004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Human phosphodiesterase 5A gene silencing device NO.2
creator: Ying Guan, Yuanbin Cui
created: 2015-08-26T11:00:00Z
modified: 2015-09-18T07:29:07Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2140_
m user id: 25248
owner id: 25248
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This device is uesd for silencing the human phosphodiesterase 5A (PDE5A) gene.A U6 promoter driving a designed, synthetic shRNA-like miRNA followed by the terminator. PDE5A is a cGMP-binding, cGMP-specific phosphodiesterase, a member of the cyclic nucleotide phosphodiesterase family. This phosphodiesterase specifically hydrolyzes cGMP to 5'-GMP. It is involved in the regulation of intracellular concentrations of cyclic nucleotides and is important for smooth muscle relaxation in the cardiovascular system. We designed 3 silencing device and test their function at the same time.Here is the another two device :BBa_K1720003,BBaK172005 This device with a GFP reporter was then transfected into HEK293 cells by lentiviral vector.Once we silence the PDE5A gene the level of cGMP will be up regulated as a result. The positive control was HEK293 cells that treat with Sodium Nitroprusside ,a NO donator that activate sGC and up regulate the level of cGMP. A negative control was made by transfecting an empty vector that does not contain sGC subunit. We used Elisa to detect cGMP level. The results are as follow:
mutable notes: 1. Kukreja RC, Salloum FN, Das A: Cyclic Guanosine Monophosphate Signaling and Phosphodiesterase-5 Inhibitors in Cardioprotection. Journal Of the American College Of Cardiology 2012, 59(22):1921-1927. 2. Li L, Haider HK, Wang L, Lu G, Ashraf M: Adenoviral short hairpin RNA therapy targeting phosphodiesterase 5a relieves cardiac remodeling and dysfunction following myocardial infarction. American Journal Of Physiology-Heart And Circulatory Physiology 2012, 302(10):H2112-H2121. 3. Unwalla HJ, Li HT, Bahner I, Li MJ, Kohn D, Rossi JJ: Novel Pol II fusion promoter directs human immunodeficiency virus type 1-inducible coexpression of a short hairpin RNA and protein. Journal of virology 2006, 80(4):1863-1873.
mutable provenance: We designed the shRNA according to the homology segment sequence of PDE5A from NCBI
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
sequence: https://synbiohub.org/public/igem/BBa_K1720004_sequence/1

BBa_K404116
type: ComponentDefinition
persistent identity: BBa K404116
display id: BBa_K404116
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: hGH_[AAV2]-right-ITR
creator: Freiburg Bioware 2010
created: 2010-10-10T11:00:00Z
modified: 2015-05-08T01:12:23Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 6733
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: right ITR
sequence annotation: hGH
sequence: https://synbiohub.org/public/igem/BBa_K404116_sequence/1

right ITR
type: SequenceAnnotation
persistent identity: annotation2093229
display id: annotation2093229
version: 1
top level: BBa_K404116
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404116/annotation2093229/range2093229/1

https://synbiohub.org/public/igem/BBa_K404116/annotation2093229/range2093229/1
type: Range
persistent identity: range2093229
display id: range2093229
version: 1
direction: direction/forward
top level: BBa_K404116
start: 495
end: 632
orientation: inline

hGH
type: SequenceAnnotation
persistent identity: annotation2093227
display id: annotation2093227
version: 1
top level: BBa_K404116
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404116/annotation2093227/range2093227/1

https://synbiohub.org/public/igem/BBa_K404116/annotation2093227/range2093227/1
type: Range
persistent identity: range2093227
display id: range2093227
version: 1
direction: direction/forward
top level: BBa_K404116
start: 1
end: 482
orientation: inline

BBa_K404127
type: ComponentDefinition
persistent identity: BBa K404127
display id: BBa_K404127
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: [AAV2]-left-ITR_pCMV_betaglobin_mVenus_[AAV2]-right-ITR
creator: Freiburg Bioware 2010
created: 2010-10-10T11:00:00Z
modified: 2015-05-08T01:12:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 6733
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: right ITR
sequence annotation: mVenus
sequence annotation: CMV promoter
sequence annotation: left ITR
sequence annotation: Beta-Globin-intron
sequence: https://synbiohub.org/public/igem/BBa_K404127_sequence/1

right ITR
type: SequenceAnnotation
persistent identity: annotation2093302
display id: annotation2093302
version: 1
top level: BBa_K404127
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404127/annotation2093302/range2093302/1

https://synbiohub.org/public/igem/BBa_K404127/annotation2093302/range2093302/1
type: Range
persistent identity: range2093302
display id: range2093302
version: 1
direction: direction/forward
top level: BBa_K404127
start: 2062
end: 2199
orientation: inline

mVenus
type: SequenceAnnotation
persistent identity: annotation2093301
display id: annotation2093301
version: 1
top level: BBa_K404127
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404127/annotation2093301/range2093301/1

https://synbiohub.org/public/igem/BBa_K404127/annotation2093301/range2093301/1
type: Range
persistent identity: range2093301
display id: range2093301
version: 1
direction: direction/forward
top level: BBa_K404127
start: 1325
end: 2038
orientation: inline

CMV promoter
type: SequenceAnnotation
persistent identity: annotation2093299
display id: annotation2093299
version: 1
top level: BBa_K404127
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K404127/annotation2093299/range2093299/1

https://synbiohub.org/public/igem/BBa_K404127/annotation2093299/range2093299/1
type: Range
persistent identity: range2093299
display id: range2093299
version: 1
direction: direction/forward
top level: BBa_K404127
start: 155
end: 809
orientation: inline

left ITR
type: SequenceAnnotation
persistent identity: annotation2093298
display id: annotation2093298
version: 1
top level: BBa_K404127
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404127/annotation2093298/range2093298/1

https://synbiohub.org/public/igem/BBa_K404127/annotation2093298/range2093298/1
type: Range
persistent identity: range2093298
display id: range2093298
version: 1
direction: direction/forward
top level: BBa_K404127
start: 1
end: 147
orientation: inline

Beta-Globin-intron
type: SequenceAnnotation
persistent identity: annotation2093300
display id: annotation2093300
version: 1
top level: BBa_K404127
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404127/annotation2093300/range2093300/1

https://synbiohub.org/public/igem/BBa_K404127/annotation2093300/range2093300/1
type: Range
persistent identity: range2093300
display id: range2093300
version: 1
direction: direction/forward
top level: BBa_K404127
start: 816
end: 1310
orientation: inline

BBa_K678004
type: ComponentDefinition
persistent identity: BBa K678004
display id: BBa_K678004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PGK, mammalian promoter
creator: DTU-Denmark-2
created: 2011-09-03T11:00:00Z
modified: 2015-05-08T01:13:04Z
discontinued: false
dominant: false
experience: experience/None
group u list: _882_
m user id: 0
owner id: 8341
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Promoter for expression of genes in mammalian cells
mutable notes: Forward direction
mutable provenance: From plasmid pSuperior.puro from ￼￼pSuper RNAi System, OligoEngine
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: Linker 1
sequence annotation: PGK
sequence: https://synbiohub.org/public/igem/BBa_K678004_sequence/1

Linker 1
type: SequenceAnnotation
persistent identity: annotation2127344
display id: annotation2127344
version: 1
top level: BBa_K678004
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K678004/annotation2127344/range2127344/1

https://synbiohub.org/public/igem/BBa_K678004/annotation2127344/range2127344/1
type: Range
persistent identity: range2127344
display id: range2127344
version: 1
direction: direction/forward
top level: BBa_K678004
start: 1
end: 8
orientation: inline

PGK
type: SequenceAnnotation
persistent identity: annotation2127345
display id: annotation2127345
version: 1
top level: BBa_K678004
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K678004/annotation2127345/range2127345/1

https://synbiohub.org/public/igem/BBa_K678004/annotation2127345/range2127345/1
type: Range
persistent identity: range2127345
display id: range2127345
version: 1
direction: direction/forward
top level: BBa_K678004
start: 9
end: 407
orientation: inline

BBa_K1926001
type: ComponentDefinition
persistent identity: BBa K1926001
display id: BBa_K1926001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: A cyclic promoter of Cyclin E from human genome
creator: Xinyi Liu
created: 2016-10-08T11:00:00Z
modified: 2016-10-19T01:15:47Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2393_
m user id: 28998
owner id: 28998
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This part is a cyclic promoter named Cyclin E from human genome. It can lead to transcription of the downstream DNA sequence once in every G1 phase[1,2] 1. Hwang, H.C. and B.E. Clurman, Cyclin E in normal and neoplastic cell cycles. Oncogene, 2005. 24(17): p. 2776-86. 2. Ohtani, K., J. DeGregori, and J.R. Nevins, Regulation of the cyclin E gene by transcription factor E2F1. Proc Natl Acad Sci U S A, 1995. 92(26): p. 12146-50.
mutable notes: None
mutable provenance: The sequence was retrieved from Addgene. We got it from human genome through PCR using the following primers: pCCNE-F: CGTGTTTACATTCCACCCGCGCCA pCCNE-R: TGATGGGGCTGCTCCGGCCT (Product: 986)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: P CCNE
sequence: https://synbiohub.org/public/igem/BBa_K1926001_sequence/1

P CCNE
type: SequenceAnnotation
persistent identity: annotation2491691
display id: annotation2491691
version: 1
top level: BBa_K1926001
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1926001/annotation2491691/range2491691/1

https://synbiohub.org/public/igem/BBa_K1926001/annotation2491691/range2491691/1
type: Range
persistent identity: range2491691
display id: range2491691
version: 1
direction: direction/forward
top level: BBa_K1926001
start: 65
end: 1049
orientation: inline

BBa_K404123
type: ComponentDefinition
persistent identity: BBa K404123
display id: BBa_K404123
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: [AAV2]-left-ITR_phTERT_betaglobin_mVenus_hGH_[AAV2]-right-ITR
creator: Freiburg Bioware 2010
created: 2010-10-10T11:00:00Z
modified: 2015-05-08T01:12:23Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 6733
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: phTERT promoter
sequence annotation: left ITR
sequence annotation: hGH
sequence annotation: right ITR
sequence annotation: mVenus
sequence annotation: Beta-Globin-intron
sequence: https://synbiohub.org/public/igem/BBa_K404123_sequence/1

phTERT promoter
type: SequenceAnnotation
persistent identity: annotation2093276
display id: annotation2093276
version: 1
top level: BBa_K404123
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K404123/annotation2093276/range2093276/1

https://synbiohub.org/public/igem/BBa_K404123/annotation2093276/range2093276/1
type: Range
persistent identity: range2093276
display id: range2093276
version: 1
direction: direction/forward
top level: BBa_K404123
start: 154
end: 612
orientation: inline

left ITR
type: SequenceAnnotation
persistent identity: annotation2093275
display id: annotation2093275
version: 1
top level: BBa_K404123
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404123/annotation2093275/range2093275/1

https://synbiohub.org/public/igem/BBa_K404123/annotation2093275/range2093275/1
type: Range
persistent identity: range2093275
display id: range2093275
version: 1
direction: direction/forward
top level: BBa_K404123
start: 1
end: 147
orientation: inline

hGH
type: SequenceAnnotation
persistent identity: annotation2093279
display id: annotation2093279
version: 1
top level: BBa_K404123
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404123/annotation2093279/range2093279/1

https://synbiohub.org/public/igem/BBa_K404123/annotation2093279/range2093279/1
type: Range
persistent identity: range2093279
display id: range2093279
version: 1
direction: direction/forward
top level: BBa_K404123
start: 1862
end: 2337
orientation: inline

right ITR
type: SequenceAnnotation
persistent identity: annotation2093280
display id: annotation2093280
version: 1
top level: BBa_K404123
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404123/annotation2093280/range2093280/1

https://synbiohub.org/public/igem/BBa_K404123/annotation2093280/range2093280/1
type: Range
persistent identity: range2093280
display id: range2093280
version: 1
direction: direction/forward
top level: BBa_K404123
start: 2352
end: 2489
orientation: inline

mVenus
type: SequenceAnnotation
persistent identity: annotation2093278
display id: annotation2093278
version: 1
top level: BBa_K404123
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404123/annotation2093278/range2093278/1

https://synbiohub.org/public/igem/BBa_K404123/annotation2093278/range2093278/1
type: Range
persistent identity: range2093278
display id: range2093278
version: 1
direction: direction/forward
top level: BBa_K404123
start: 1128
end: 1841
orientation: inline

Beta-Globin-intron
type: SequenceAnnotation
persistent identity: annotation2093277
display id: annotation2093277
version: 1
top level: BBa_K404123
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404123/annotation2093277/range2093277/1

https://synbiohub.org/public/igem/BBa_K404123/annotation2093277/range2093277/1
type: Range
persistent identity: range2093277
display id: range2093277
version: 1
direction: direction/forward
top level: BBa_K404123
start: 619
end: 1113
orientation: inline

BBa_K404241
type: ComponentDefinition
persistent identity: BBa K404241
display id: BBa_K404241
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pCMV_[AAV2]VP123 (ViralBrick-453-BAP)
creator: Freiburg Bioware 2010
created: 2010-09-19T11:00:00Z
modified: 2015-05-08T01:12:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 7049
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: PLA2
sequence annotation: BAP
sequence annotation: CMV
sequence annotation: BR3
sequence annotation: BR2
sequence annotation: VP1
sequence annotation: A-spacer
sequence annotation: VP3
sequence annotation: BR4
sequence annotation: VP2
sequence annotation: BR1
sequence annotation: A-Spacer
sequence annotation: AAV-2-cap (Stratagene)
sequence: https://synbiohub.org/public/igem/BBa_K404241_sequence/1

PLA2
type: SequenceAnnotation
persistent identity: annotation2094196
display id: annotation2094196
version: 1
top level: BBa_K404241
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404241/annotation2094196/range2094196/1

https://synbiohub.org/public/igem/BBa_K404241/annotation2094196/range2094196/1
type: Range
persistent identity: range2094196
display id: range2094196
version: 1
direction: direction/forward
top level: BBa_K404241
start: 799
end: 976
orientation: inline

BAP
type: SequenceAnnotation
persistent identity: annotation2094204
display id: annotation2094204
version: 1
top level: BBa_K404241
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404241/annotation2094204/range2094204/1

https://synbiohub.org/public/igem/BBa_K404241/annotation2094204/range2094204/1
type: Range
persistent identity: range2094204
display id: range2094204
version: 1
direction: direction/forward
top level: BBa_K404241
start: 2030
end: 2074
orientation: inline

CMV
type: SequenceAnnotation
persistent identity: annotation2094193
display id: annotation2094193
version: 1
top level: BBa_K404241
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K404241/annotation2094193/range2094193/1

https://synbiohub.org/public/igem/BBa_K404241/annotation2094193/range2094193/1
type: Range
persistent identity: range2094193
display id: range2094193
version: 1
direction: direction/forward
top level: BBa_K404241
start: 2
end: 655
orientation: inline

BR3
type: SequenceAnnotation
persistent identity: annotation2094200
display id: annotation2094200
version: 1
top level: BBa_K404241
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404241/annotation2094200/range2094200/1

https://synbiohub.org/public/igem/BBa_K404241/annotation2094200/range2094200/1
type: Range
persistent identity: range2094200
display id: range2094200
version: 1
direction: direction/forward
top level: BBa_K404241
start: 1163
end: 1183
orientation: inline

BR2
type: SequenceAnnotation
persistent identity: annotation2094199
display id: annotation2094199
version: 1
top level: BBa_K404241
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404241/annotation2094199/range2094199/1

https://synbiohub.org/public/igem/BBa_K404241/annotation2094199/range2094199/1
type: Range
persistent identity: range2094199
display id: range2094199
version: 1
direction: direction/forward
top level: BBa_K404241
start: 1085
end: 1105
orientation: inline

VP1
type: SequenceAnnotation
persistent identity: annotation2094195
display id: annotation2094195
version: 1
top level: BBa_K404241
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404241/annotation2094195/range2094195/1

https://synbiohub.org/public/igem/BBa_K404241/annotation2094195/range2094195/1
type: Range
persistent identity: range2094195
display id: range2094195
version: 1
direction: direction/forward
top level: BBa_K404241
start: 671
end: 2926
orientation: inline

A-spacer
type: SequenceAnnotation
persistent identity: annotation2094191
display id: annotation2094191
version: 1
top level: BBa_K404241
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404241/annotation2094191/range2094191/1

https://synbiohub.org/public/igem/BBa_K404241/annotation2094191/range2094191/1
type: Range
persistent identity: range2094191
display id: range2094191
version: 1
direction: direction/forward
top level: BBa_K404241
start: 2075
end: 2077
orientation: inline

VP3
type: SequenceAnnotation
persistent identity: annotation2094201
display id: annotation2094201
version: 1
top level: BBa_K404241
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404241/annotation2094201/range2094201/1

https://synbiohub.org/public/igem/BBa_K404241/annotation2094201/range2094201/1
type: Range
persistent identity: range2094201
display id: range2094201
version: 1
direction: direction/forward
top level: BBa_K404241
start: 1274
end: 2926
orientation: inline

BR4
type: SequenceAnnotation
persistent identity: annotation2094202
display id: annotation2094202
version: 1
top level: BBa_K404241
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404241/annotation2094202/range2094202/1

https://synbiohub.org/public/igem/BBa_K404241/annotation2094202/range2094202/1
type: Range
persistent identity: range2094202
display id: range2094202
version: 1
direction: direction/forward
top level: BBa_K404241
start: 1586
end: 1603
orientation: inline

VP2
type: SequenceAnnotation
persistent identity: annotation2094198
display id: annotation2094198
version: 1
top level: BBa_K404241
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404241/annotation2094198/range2094198/1

https://synbiohub.org/public/igem/BBa_K404241/annotation2094198/range2094198/1
type: Range
persistent identity: range2094198
display id: range2094198
version: 1
direction: direction/forward
top level: BBa_K404241
start: 1079
end: 2926
orientation: inline

BR1
type: SequenceAnnotation
persistent identity: annotation2094197
display id: annotation2094197
version: 1
top level: BBa_K404241
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404241/annotation2094197/range2094197/1

https://synbiohub.org/public/igem/BBa_K404241/annotation2094197/range2094197/1
type: Range
persistent identity: range2094197
display id: range2094197
version: 1
direction: direction/forward
top level: BBa_K404241
start: 1025
end: 1045
orientation: inline

A-Spacer
type: SequenceAnnotation
persistent identity: annotation2094203
display id: annotation2094203
version: 1
top level: BBa_K404241
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404241/annotation2094203/range2094203/1

https://synbiohub.org/public/igem/BBa_K404241/annotation2094203/range2094203/1
type: Range
persistent identity: range2094203
display id: range2094203
version: 1
direction: direction/forward
top level: BBa_K404241
start: 2027
end: 2029
orientation: inline

AAV-2-cap (Stratagene)
type: SequenceAnnotation
persistent identity: annotation2094194
display id: annotation2094194
version: 1
top level: BBa_K404241
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404241/annotation2094194/range2094194/1

https://synbiohub.org/public/igem/BBa_K404241/annotation2094194/range2094194/1
type: Range
persistent identity: range2094194
display id: range2094194
version: 1
direction: direction/forward
top level: BBa_K404241
start: 671
end: 3049
orientation: inline

hPCD
type: ComponentDefinition
persistent identity: BBa J176000
display id: BBa_J176000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: hPCD
creator: Karmella Haynes
created: 2011-06-23T11:00:00Z
modified: 2015-08-31T04:08:33Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _863_
m user id: 0
owner id: 1144
owning group id: 61
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Human Polycomb chromodomain from the CBX8 protein (a.a. 1-63). See Haynes and Silver, JBC, 2011
mutable notes: The sequence was not modified.
mutable provenance: Synthesized by GenScript.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence: https://synbiohub.org/public/igem/BBa_J176000_sequence/1

BBa_K1926012
type: ComponentDefinition
persistent identity: BBa K1926012
display id: BBa_K1926012
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: The VIKA UNIT: recombinase VIKA flanked by Vox
creator: Xinyi Liu
created: 2016-10-08T11:00:00Z
modified: 2016-10-11T03:47:23Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2393_
m user id: 28998
owner id: 28998
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This part is the VIKA UNIT of cyclebow system including an sv40 nuclear location sequence (NLS), a recombinase gene named VIKA and an sv40 terminator flanked by homodromous recognition site of recombinase VIKA named Vox. The whole part can be cut off by VIKA. This part is the improvement of the BBa_K1641003，which is a plain VIKA. You may use this part to: 1) Test the cut-off efficiency of recombinase VIKA by qRT-PCR; 2) Fast cut off this part by transient transfecting recombinase gene into nucleus; 3) Do lineage tracking.
mutable notes: None
mutable provenance: The recombinase gene VIKA and its RTS Vox were got through denovo synthesis. NLS and sv40 terminator were got from commercial plasmid: pentry nls GFP/pcdn3.1.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: Vox
sequence annotation: sv40 terminator
sequence annotation: TAA
sequence annotation: VIKA
sequence: https://synbiohub.org/public/igem/BBa_K1926012_sequence/1

Vox
type: SequenceAnnotation
persistent identity: annotation2491856
display id: annotation2491856
version: 1
top level: BBa_K1926012
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1926012/annotation2491856/range2491856/1

https://synbiohub.org/public/igem/BBa_K1926012/annotation2491856/range2491856/1
type: Range
persistent identity: range2491856
display id: range2491856
version: 1
direction: direction/forward
top level: BBa_K1926012
start: 1261
end: 1294
orientation: inline

Vox
type: SequenceAnnotation
persistent identity: annotation2491854
display id: annotation2491854
version: 1
top level: BBa_K1926012
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1926012/annotation2491854/range2491854/1

https://synbiohub.org/public/igem/BBa_K1926012/annotation2491854/range2491854/1
type: Range
persistent identity: range2491854
display id: range2491854
version: 1
direction: direction/forward
top level: BBa_K1926012
start: 1
end: 34
orientation: inline

sv40 terminator
type: SequenceAnnotation
persistent identity: annotation2491857
display id: annotation2491857
version: 1
top level: BBa_K1926012
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1926012/annotation2491857/range2491857/1

https://synbiohub.org/public/igem/BBa_K1926012/annotation2491857/range2491857/1
type: Range
persistent identity: range2491857
display id: range2491857
version: 1
direction: direction/forward
top level: BBa_K1926012
start: 1118
end: 1260
orientation: inline

TAA
type: SequenceAnnotation
persistent identity: annotation2491858
display id: annotation2491858
version: 1
top level: BBa_K1926012
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1926012/annotation2491858/range2491858/1

https://synbiohub.org/public/igem/BBa_K1926012/annotation2491858/range2491858/1
type: Range
persistent identity: range2491858
display id: range2491858
version: 1
direction: direction/forward
top level: BBa_K1926012
start: 1139
end: 1141
orientation: inline

VIKA
type: SequenceAnnotation
persistent identity: annotation2491855
display id: annotation2491855
version: 1
top level: BBa_K1926012
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1926012/annotation2491855/range2491855/1

https://synbiohub.org/public/igem/BBa_K1926012/annotation2491855/range2491855/1
type: Range
persistent identity: range2491855
display id: range2491855
version: 1
direction: direction/forward
top level: BBa_K1926012
start: 35
end: 1117
orientation: inline

BBa_K404153
type: ComponentDefinition
persistent identity: BBa K404153
display id: BBa_K404153
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: [AAV2]-NLS
creator: Freiburg Bioware 2010
created: 2010-10-10T11:00:00Z
modified: 2015-05-08T01:12:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 6733
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: NLS
sequence: https://synbiohub.org/public/igem/BBa_K404153_sequence/1

NLS
type: SequenceAnnotation
persistent identity: annotation2094093
display id: annotation2094093
version: 1
top level: BBa_K404153
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404153/annotation2094093/range2094093/1

https://synbiohub.org/public/igem/BBa_K404153/annotation2094093/range2094093/1
type: Range
persistent identity: range2094093
display id: range2094093
version: 1
direction: direction/forward
top level: BBa_K404153
start: 1
end: 21
orientation: inline

BBa_K404129
type: ComponentDefinition
persistent identity: BBa K404129
display id: BBa_K404129
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: [AAV2]-left-ITR_phTERT_betaglobin_mCherry_hGH_[AAV2]-right-ITR
creator: Freiburg Bioware 2010
created: 2010-10-10T11:00:00Z
modified: 2015-05-08T01:12:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 6733
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: mCherry
sequence annotation: stem loop early T7
sequence annotation: Beta-Globin-intron
sequence annotation: right ITR
sequence annotation: phTERT
sequence annotation: left ITR
sequence annotation: hGH terminator
sequence annotation: rrnB _T1
sequence: https://synbiohub.org/public/igem/BBa_K404129_sequence/1

mCherry
type: SequenceAnnotation
persistent identity: annotation2093319
display id: annotation2093319
version: 1
top level: BBa_K404129
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404129/annotation2093319/range2093319/1

https://synbiohub.org/public/igem/BBa_K404129/annotation2093319/range2093319/1
type: Range
persistent identity: range2093319
display id: range2093319
version: 1
direction: direction/forward
top level: BBa_K404129
start: 1139
end: 1849
orientation: inline

stem loop early T7
type: SequenceAnnotation
persistent identity: annotation2093336
display id: annotation2093336
version: 1
top level: BBa_K404129
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404129/annotation2093336/range2093336/1

https://synbiohub.org/public/igem/BBa_K404129/annotation2093336/range2093336/1
type: Range
persistent identity: range2093336
display id: range2093336
version: 1
direction: direction/forward
top level: BBa_K404129
start: 1956
end: 1975
orientation: inline

Beta-Globin-intron
type: SequenceAnnotation
persistent identity: annotation2093318
display id: annotation2093318
version: 1
top level: BBa_K404129
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404129/annotation2093318/range2093318/1

https://synbiohub.org/public/igem/BBa_K404129/annotation2093318/range2093318/1
type: Range
persistent identity: range2093318
display id: range2093318
version: 1
direction: direction/forward
top level: BBa_K404129
start: 619
end: 1113
orientation: inline

right ITR
type: SequenceAnnotation
persistent identity: annotation2093320
display id: annotation2093320
version: 1
top level: BBa_K404129
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404129/annotation2093320/range2093320/1

https://synbiohub.org/public/igem/BBa_K404129/annotation2093320/range2093320/1
type: Range
persistent identity: range2093320
display id: range2093320
version: 1
direction: direction/forward
top level: BBa_K404129
start: 2491
end: 2629
orientation: inline

phTERT
type: SequenceAnnotation
persistent identity: annotation2093316
display id: annotation2093316
version: 1
top level: BBa_K404129
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K404129/annotation2093316/range2093316/1

https://synbiohub.org/public/igem/BBa_K404129/annotation2093316/range2093316/1
type: Range
persistent identity: range2093316
display id: range2093316
version: 1
direction: direction/forward
top level: BBa_K404129
start: 154
end: 612
orientation: inline

left ITR
type: SequenceAnnotation
persistent identity: annotation2093315
display id: annotation2093315
version: 1
top level: BBa_K404129
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404129/annotation2093315/range2093315/1

https://synbiohub.org/public/igem/BBa_K404129/annotation2093315/range2093315/1
type: Range
persistent identity: range2093315
display id: range2093315
version: 1
direction: direction/forward
top level: BBa_K404129
start: 1
end: 147
orientation: inline

hGH terminator
type: SequenceAnnotation
persistent identity: annotation2093321
display id: annotation2093321
version: 1
top level: BBa_K404129
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404129/annotation2093321/range2093321/1

https://synbiohub.org/public/igem/BBa_K404129/annotation2093321/range2093321/1
type: Range
persistent identity: range2093321
display id: range2093321
version: 1
direction: direction/forward
top level: BBa_K404129
start: 1998
end: 2476
orientation: inline

rrnB _T1
type: SequenceAnnotation
persistent identity: annotation2093335
display id: annotation2093335
version: 1
top level: BBa_K404129
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404129/annotation2093335/range2093335/1

https://synbiohub.org/public/igem/BBa_K404129/annotation2093335/range2093335/1
type: Range
persistent identity: range2093335
display id: range2093335
version: 1
direction: direction/forward
top level: BBa_K404129
start: 1872
end: 1916
orientation: inline

BBa_K404120
type: ComponentDefinition
persistent identity: BBa K404120
display id: BBa_K404120
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: [AAV2]-left-ITR_pCMV_betaglobin_CFP_hGH_[AAV2]-right-ITR
creator: Freiburg Bioware 2010
created: 2010-10-10T11:00:00Z
modified: 2015-05-08T01:12:23Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 6733
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: Beta-Globin-intron
sequence annotation: CFP
sequence annotation: right ITR
sequence annotation: left ITR
sequence annotation: hGH
sequence annotation: CMV promoter
sequence: https://synbiohub.org/public/igem/BBa_K404120_sequence/1

Beta-Globin-intron
type: SequenceAnnotation
persistent identity: annotation2093246
display id: annotation2093246
version: 1
top level: BBa_K404120
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404120/annotation2093246/range2093246/1

https://synbiohub.org/public/igem/BBa_K404120/annotation2093246/range2093246/1
type: Range
persistent identity: range2093246
display id: range2093246
version: 1
direction: direction/forward
top level: BBa_K404120
start: 816
end: 1310
orientation: inline

CFP
type: SequenceAnnotation
persistent identity: annotation2093247
display id: annotation2093247
version: 1
top level: BBa_K404120
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404120/annotation2093247/range2093247/1

https://synbiohub.org/public/igem/BBa_K404120/annotation2093247/range2093247/1
type: Range
persistent identity: range2093247
display id: range2093247
version: 1
direction: direction/forward
top level: BBa_K404120
start: 1325
end: 2038
orientation: inline

right ITR
type: SequenceAnnotation
persistent identity: annotation2093258
display id: annotation2093258
version: 1
top level: BBa_K404120
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404120/annotation2093258/range2093258/1

https://synbiohub.org/public/igem/BBa_K404120/annotation2093258/range2093258/1
type: Range
persistent identity: range2093258
display id: range2093258
version: 1
direction: direction/forward
top level: BBa_K404120
start: 2549
end: 2686
orientation: inline

left ITR
type: SequenceAnnotation
persistent identity: annotation2093244
display id: annotation2093244
version: 1
top level: BBa_K404120
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404120/annotation2093244/range2093244/1

https://synbiohub.org/public/igem/BBa_K404120/annotation2093244/range2093244/1
type: Range
persistent identity: range2093244
display id: range2093244
version: 1
direction: direction/forward
top level: BBa_K404120
start: 1
end: 147
orientation: inline

hGH
type: SequenceAnnotation
persistent identity: annotation2093257
display id: annotation2093257
version: 1
top level: BBa_K404120
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404120/annotation2093257/range2093257/1

https://synbiohub.org/public/igem/BBa_K404120/annotation2093257/range2093257/1
type: Range
persistent identity: range2093257
display id: range2093257
version: 1
direction: direction/forward
top level: BBa_K404120
start: 2056
end: 2534
orientation: inline

CMV promoter
type: SequenceAnnotation
persistent identity: annotation2093245
display id: annotation2093245
version: 1
top level: BBa_K404120
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K404120/annotation2093245/range2093245/1

https://synbiohub.org/public/igem/BBa_K404120/annotation2093245/range2093245/1
type: Range
persistent identity: range2093245
display id: range2093245
version: 1
direction: direction/forward
top level: BBa_K404120
start: 155
end: 809
orientation: inline

BBa_K404119
type: ComponentDefinition
persistent identity: BBa K404119
display id: BBa_K404119
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: [AAV2]-left-ITR_pCMV_betaglobin_mVenus_hGH_[AAV2]-right-ITR
creator: Freiburg Bioware 2010
created: 2010-10-10T11:00:00Z
modified: 2015-05-08T01:12:23Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 6733
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: left ITR
sequence annotation: Beta-Globin-intron
sequence annotation: CMV promoter
sequence annotation: right ITR
sequence annotation: mVenus
sequence annotation: hGH
sequence: https://synbiohub.org/public/igem/BBa_K404119_sequence/1

left ITR
type: SequenceAnnotation
persistent identity: annotation2093237
display id: annotation2093237
version: 1
top level: BBa_K404119
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404119/annotation2093237/range2093237/1

https://synbiohub.org/public/igem/BBa_K404119/annotation2093237/range2093237/1
type: Range
persistent identity: range2093237
display id: range2093237
version: 1
direction: direction/forward
top level: BBa_K404119
start: 1
end: 147
orientation: inline

Beta-Globin-intron
type: SequenceAnnotation
persistent identity: annotation2093239
display id: annotation2093239
version: 1
top level: BBa_K404119
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404119/annotation2093239/range2093239/1

https://synbiohub.org/public/igem/BBa_K404119/annotation2093239/range2093239/1
type: Range
persistent identity: range2093239
display id: range2093239
version: 1
direction: direction/forward
top level: BBa_K404119
start: 816
end: 1310
orientation: inline

CMV promoter
type: SequenceAnnotation
persistent identity: annotation2093238
display id: annotation2093238
version: 1
top level: BBa_K404119
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K404119/annotation2093238/range2093238/1

https://synbiohub.org/public/igem/BBa_K404119/annotation2093238/range2093238/1
type: Range
persistent identity: range2093238
display id: range2093238
version: 1
direction: direction/forward
top level: BBa_K404119
start: 155
end: 808
orientation: inline

right ITR
type: SequenceAnnotation
persistent identity: annotation2093242
display id: annotation2093242
version: 1
top level: BBa_K404119
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404119/annotation2093242/range2093242/1

https://synbiohub.org/public/igem/BBa_K404119/annotation2093242/range2093242/1
type: Range
persistent identity: range2093242
display id: range2093242
version: 1
direction: direction/forward
top level: BBa_K404119
start: 2549
end: 2686
orientation: inline

mVenus
type: SequenceAnnotation
persistent identity: annotation2093241
display id: annotation2093241
version: 1
top level: BBa_K404119
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404119/annotation2093241/range2093241/1

https://synbiohub.org/public/igem/BBa_K404119/annotation2093241/range2093241/1
type: Range
persistent identity: range2093241
display id: range2093241
version: 1
direction: direction/forward
top level: BBa_K404119
start: 1325
end: 2038
orientation: inline

hGH
type: SequenceAnnotation
persistent identity: annotation2093240
display id: annotation2093240
version: 1
top level: BBa_K404119
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404119/annotation2093240/range2093240/1

https://synbiohub.org/public/igem/BBa_K404119/annotation2093240/range2093240/1
type: Range
persistent identity: range2093240
display id: range2093240
version: 1
direction: direction/forward
top level: BBa_K404119
start: 2056
end: 2535
orientation: inline

BBa_K404011
type: ComponentDefinition
persistent identity: BBa K404011
display id: BBa_K404011
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pCMV_[AAV2]-VP2
creator: Freiburg Bioware 2010
created: 2010-10-20T11:00:00Z
modified: 2015-05-08T01:12:23Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 6674
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: VP2
sequence annotation: CMV
sequence: https://synbiohub.org/public/igem/BBa_K404011_sequence/1

VP2
type: SequenceAnnotation
persistent identity: annotation2097904
display id: annotation2097904
version: 1
top level: BBa_K404011
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404011/annotation2097904/range2097904/1

https://synbiohub.org/public/igem/BBa_K404011/annotation2097904/range2097904/1
type: Range
persistent identity: range2097904
display id: range2097904
version: 1
direction: direction/forward
top level: BBa_K404011
start: 670
end: 2500
orientation: inline

CMV
type: SequenceAnnotation
persistent identity: annotation2097903
display id: annotation2097903
version: 1
top level: BBa_K404011
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K404011/annotation2097903/range2097903/1

https://synbiohub.org/public/igem/BBa_K404011/annotation2097903/range2097903/1
type: Range
persistent identity: range2097903
display id: range2097903
version: 1
direction: direction/forward
top level: BBa_K404011
start: 2
end: 655
orientation: inline

BBa_K404244
type: ComponentDefinition
persistent identity: BBa K404244
display id: BBa_K404244
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pCMV_[AAV2]VP123 (ViralBrick-453-Z34C)
creator: Freiburg Bioware 2010
created: 2010-10-13T11:00:00Z
modified: 2015-05-08T01:12:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 7049
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: VP3
sequence annotation: VP2
sequence annotation: AAV-2-cap (Strategene)
sequence annotation: Z34C
sequence annotation: VP1
sequence annotation: CMV promoter
sequence: https://synbiohub.org/public/igem/BBa_K404244_sequence/1

VP3
type: SequenceAnnotation
persistent identity: annotation2094257
display id: annotation2094257
version: 1
top level: BBa_K404244
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404244/annotation2094257/range2094257/1

https://synbiohub.org/public/igem/BBa_K404244/annotation2094257/range2094257/1
type: Range
persistent identity: range2094257
display id: range2094257
version: 1
direction: direction/forward
top level: BBa_K404244
start: 1274
end: 2977
orientation: inline

VP2
type: SequenceAnnotation
persistent identity: annotation2094256
display id: annotation2094256
version: 1
top level: BBa_K404244
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404244/annotation2094256/range2094256/1

https://synbiohub.org/public/igem/BBa_K404244/annotation2094256/range2094256/1
type: Range
persistent identity: range2094256
display id: range2094256
version: 1
direction: direction/forward
top level: BBa_K404244
start: 1079
end: 2977
orientation: inline

AAV-2-cap (Strategene)
type: SequenceAnnotation
persistent identity: annotation2094258
display id: annotation2094258
version: 1
top level: BBa_K404244
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404244/annotation2094258/range2094258/1

https://synbiohub.org/public/igem/BBa_K404244/annotation2094258/range2094258/1
type: Range
persistent identity: range2094258
display id: range2094258
version: 1
direction: direction/forward
top level: BBa_K404244
start: 671
end: 3100
orientation: inline

Z34C
type: SequenceAnnotation
persistent identity: annotation2094259
display id: annotation2094259
version: 1
top level: BBa_K404244
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404244/annotation2094259/range2094259/1

https://synbiohub.org/public/igem/BBa_K404244/annotation2094259/range2094259/1
type: Range
persistent identity: range2094259
display id: range2094259
version: 1
direction: direction/forward
top level: BBa_K404244
start: 2027
end: 2128
orientation: inline

VP1
type: SequenceAnnotation
persistent identity: annotation2094255
display id: annotation2094255
version: 1
top level: BBa_K404244
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404244/annotation2094255/range2094255/1

https://synbiohub.org/public/igem/BBa_K404244/annotation2094255/range2094255/1
type: Range
persistent identity: range2094255
display id: range2094255
version: 1
direction: direction/forward
top level: BBa_K404244
start: 671
end: 2977
orientation: inline

CMV promoter
type: SequenceAnnotation
persistent identity: annotation2094254
display id: annotation2094254
version: 1
top level: BBa_K404244
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K404244/annotation2094254/range2094254/1

https://synbiohub.org/public/igem/BBa_K404244/annotation2094254/range2094254/1
type: Range
persistent identity: range2094254
display id: range2094254
version: 1
direction: direction/forward
top level: BBa_K404244
start: 2
end: 655
orientation: inline

BBa_K404256
type: ComponentDefinition
persistent identity: BBa K404256
display id: BBa_K404256
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pCMV_[AAV2]-VP123ex (ViralBrick-587KO-Z34C-Spacer)
creator: Freiburg Bioware 2010
created: 2010-10-20T11:00:00Z
modified: 2015-05-08T01:12:25Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 6674
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: upstream 587
sequence annotation: Z34C
sequence annotation: HSPG-KO
sequence annotation: downstream 587
sequence annotation: CMV
sequence annotation: TG-spacer
sequence annotation: VP2
sequence annotation: VP1
sequence annotation: VP3
sequence annotation: GLS-spacer
sequence annotation: AAV--1-cap (Stratagene)
sequence: https://synbiohub.org/public/igem/BBa_K404256_sequence/1

upstream 587
type: SequenceAnnotation
persistent identity: annotation2094736
display id: annotation2094736
version: 1
top level: BBa_K404256
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404256/annotation2094736/range2094736/1

https://synbiohub.org/public/igem/BBa_K404256/annotation2094736/range2094736/1
type: Range
persistent identity: range2094736
display id: range2094736
version: 1
direction: direction/forward
top level: BBa_K404256
start: 2397
end: 2428
orientation: inline

Z34C
type: SequenceAnnotation
persistent identity: annotation2094731
display id: annotation2094731
version: 1
top level: BBa_K404256
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404256/annotation2094731/range2094731/1

https://synbiohub.org/public/igem/BBa_K404256/annotation2094731/range2094731/1
type: Range
persistent identity: range2094731
display id: range2094731
version: 1
direction: direction/forward
top level: BBa_K404256
start: 2435
end: 2536
orientation: inline

HSPG-KO
type: SequenceAnnotation
persistent identity: annotation2101929
display id: annotation2101929
version: 1
top level: BBa_K404256
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K404256/annotation2101929/range2101929/1

https://synbiohub.org/public/igem/BBa_K404256/annotation2101929/range2101929/1
type: Range
persistent identity: range2101929
display id: range2101929
version: 1
direction: direction/forward
top level: BBa_K404256
start: 2433
end: 2435
orientation: inline

downstream 587
type: SequenceAnnotation
persistent identity: annotation2094735
display id: annotation2094735
version: 1
top level: BBa_K404256
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404256/annotation2094735/range2094735/1

https://synbiohub.org/public/igem/BBa_K404256/annotation2094735/range2094735/1
type: Range
persistent identity: range2094735
display id: range2094735
version: 1
direction: direction/forward
top level: BBa_K404256
start: 2546
end: 2561
orientation: inline

CMV
type: SequenceAnnotation
persistent identity: annotation2094726
display id: annotation2094726
version: 1
top level: BBa_K404256
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K404256/annotation2094726/range2094726/1

https://synbiohub.org/public/igem/BBa_K404256/annotation2094726/range2094726/1
type: Range
persistent identity: range2094726
display id: range2094726
version: 1
direction: direction/forward
top level: BBa_K404256
start: 2
end: 655
orientation: inline

HSPG-KO
type: SequenceAnnotation
persistent identity: annotation2101894
display id: annotation2101894
version: 1
top level: BBa_K404256
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K404256/annotation2101894/range2101894/1

https://synbiohub.org/public/igem/BBa_K404256/annotation2101894/range2101894/1
type: Range
persistent identity: range2101894
display id: range2101894
version: 1
direction: direction/forward
top level: BBa_K404256
start: 2546
end: 2548
orientation: inline

TG-spacer
type: SequenceAnnotation
persistent identity: annotation2094733
display id: annotation2094733
version: 1
top level: BBa_K404256
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404256/annotation2094733/range2094733/1

https://synbiohub.org/public/igem/BBa_K404256/annotation2094733/range2094733/1
type: Range
persistent identity: range2094733
display id: range2094733
version: 1
direction: direction/forward
top level: BBa_K404256
start: 2429
end: 2434
orientation: inline

VP2
type: SequenceAnnotation
persistent identity: annotation2094728
display id: annotation2094728
version: 1
top level: BBa_K404256
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404256/annotation2094728/range2094728/1

https://synbiohub.org/public/igem/BBa_K404256/annotation2094728/range2094728/1
type: Range
persistent identity: range2094728
display id: range2094728
version: 1
direction: direction/forward
top level: BBa_K404256
start: 1079
end: 2992
orientation: inline

VP1
type: SequenceAnnotation
persistent identity: annotation2094727
display id: annotation2094727
version: 1
top level: BBa_K404256
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404256/annotation2094727/range2094727/1

https://synbiohub.org/public/igem/BBa_K404256/annotation2094727/range2094727/1
type: Range
persistent identity: range2094727
display id: range2094727
version: 1
direction: direction/forward
top level: BBa_K404256
start: 671
end: 2992
orientation: inline

VP3
type: SequenceAnnotation
persistent identity: annotation2094729
display id: annotation2094729
version: 1
top level: BBa_K404256
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404256/annotation2094729/range2094729/1

https://synbiohub.org/public/igem/BBa_K404256/annotation2094729/range2094729/1
type: Range
persistent identity: range2094729
display id: range2094729
version: 1
direction: direction/forward
top level: BBa_K404256
start: 1274
end: 2992
orientation: inline

GLS-spacer
type: SequenceAnnotation
persistent identity: annotation2094734
display id: annotation2094734
version: 1
top level: BBa_K404256
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404256/annotation2094734/range2094734/1

https://synbiohub.org/public/igem/BBa_K404256/annotation2094734/range2094734/1
type: Range
persistent identity: range2094734
display id: range2094734
version: 1
direction: direction/forward
top level: BBa_K404256
start: 2537
end: 2545
orientation: inline

AAV--1-cap (Stratagene)
type: SequenceAnnotation
persistent identity: annotation2094730
display id: annotation2094730
version: 1
top level: BBa_K404256
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404256/annotation2094730/range2094730/1

https://synbiohub.org/public/igem/BBa_K404256/annotation2094730/range2094730/1
type: Range
persistent identity: range2094730
display id: range2094730
version: 1
direction: direction/forward
top level: BBa_K404256
start: 671
end: 3115
orientation: inline

BBa_K404160
type: ComponentDefinition
persistent identity: BBa K404160
display id: BBa_K404160
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pCMV_His-Tag_Middle-Linker_[AAV2]-VP23 (ViralBrick-587KO-Empty)
creator: Freiburg Bioware 2010
created: 2010-10-11T11:00:00Z
modified: 2015-05-08T01:12:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 6733
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: His-tag
sequence annotation: VP2
sequence annotation: HSPG-KO
sequence annotation: CMV
sequence annotation: AAV-2-cap (Stratagene)
sequence annotation: middle.linker
sequence annotation: VP3
sequence: https://synbiohub.org/public/igem/BBa_K404160_sequence/1

His-tag
type: SequenceAnnotation
persistent identity: annotation2097921
display id: annotation2097921
version: 1
top level: BBa_K404160
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404160/annotation2097921/range2097921/1

https://synbiohub.org/public/igem/BBa_K404160/annotation2097921/range2097921/1
type: Range
persistent identity: range2097921
display id: range2097921
version: 1
direction: direction/forward
top level: BBa_K404160
start: 671
end: 688
orientation: inline

VP2
type: SequenceAnnotation
persistent identity: annotation2097923
display id: annotation2097923
version: 1
top level: BBa_K404160
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404160/annotation2097923/range2097923/1

https://synbiohub.org/public/igem/BBa_K404160/annotation2097923/range2097923/1
type: Range
persistent identity: range2097923
display id: range2097923
version: 1
direction: direction/forward
top level: BBa_K404160
start: 725
end: 2518
orientation: inline

HSPG-KO
type: SequenceAnnotation
persistent identity: annotation2101800
display id: annotation2101800
version: 1
top level: BBa_K404160
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K404160/annotation2101800/range2101800/1

https://synbiohub.org/public/igem/BBa_K404160/annotation2101800/range2101800/1
type: Range
persistent identity: range2101800
display id: range2101800
version: 1
direction: direction/forward
top level: BBa_K404160
start: 2063
end: 2065
orientation: inline

CMV
type: SequenceAnnotation
persistent identity: annotation2097920
display id: annotation2097920
version: 1
top level: BBa_K404160
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K404160/annotation2097920/range2097920/1

https://synbiohub.org/public/igem/BBa_K404160/annotation2097920/range2097920/1
type: Range
persistent identity: range2097920
display id: range2097920
version: 1
direction: direction/forward
top level: BBa_K404160
start: 2
end: 655
orientation: inline

AAV-2-cap (Stratagene)
type: SequenceAnnotation
persistent identity: annotation2097925
display id: annotation2097925
version: 1
top level: BBa_K404160
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404160/annotation2097925/range2097925/1

https://synbiohub.org/public/igem/BBa_K404160/annotation2097925/range2097925/1
type: Range
persistent identity: range2097925
display id: range2097925
version: 1
direction: direction/forward
top level: BBa_K404160
start: 725
end: 2641
orientation: inline

middle.linker
type: SequenceAnnotation
persistent identity: annotation2097922
display id: annotation2097922
version: 1
top level: BBa_K404160
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404160/annotation2097922/range2097922/1

https://synbiohub.org/public/igem/BBa_K404160/annotation2097922/range2097922/1
type: Range
persistent identity: range2097922
display id: range2097922
version: 1
direction: direction/forward
top level: BBa_K404160
start: 695
end: 718
orientation: inline

VP3
type: SequenceAnnotation
persistent identity: annotation2097924
display id: annotation2097924
version: 1
top level: BBa_K404160
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404160/annotation2097924/range2097924/1

https://synbiohub.org/public/igem/BBa_K404160/annotation2097924/range2097924/1
type: Range
persistent identity: range2097924
display id: range2097924
version: 1
direction: direction/forward
top level: BBa_K404160
start: 917
end: 2518
orientation: inline

HSPG-KO
type: SequenceAnnotation
persistent identity: annotation2101799
display id: annotation2101799
version: 1
top level: BBa_K404160
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K404160/annotation2101799/range2101799/1

https://synbiohub.org/public/igem/BBa_K404160/annotation2101799/range2101799/1
type: Range
persistent identity: range2101799
display id: range2101799
version: 1
direction: direction/forward
top level: BBa_K404160
start: 2072
end: 2074
orientation: inline

BBa_K404247
type: ComponentDefinition
persistent identity: BBa K404247
display id: BBa_K404247
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pCMV_[AAV2]-VP123ex (ViralBrick-587-His-Tag)
creator: Freiburg Bioware 2010
created: 2010-10-13T11:00:00Z
modified: 2015-05-08T01:12:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 7049
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: AAV-2-cap (Stratagene)
sequence annotation: VP1
sequence annotation: downstream 587
sequence annotation: VP3
sequence annotation: VP2
sequence annotation: TG-spacer
sequence annotation: upstream 587
sequence annotation: CMV promoter
sequence annotation: His-tag
sequence annotation: GLS-spacer
sequence: https://synbiohub.org/public/igem/BBa_K404247_sequence/1

AAV-2-cap (Stratagene)
type: SequenceAnnotation
persistent identity: annotation2094290
display id: annotation2094290
version: 1
top level: BBa_K404247
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404247/annotation2094290/range2094290/1

https://synbiohub.org/public/igem/BBa_K404247/annotation2094290/range2094290/1
type: Range
persistent identity: range2094290
display id: range2094290
version: 1
direction: direction/forward
top level: BBa_K404247
start: 671
end: 3031
orientation: inline

VP1
type: SequenceAnnotation
persistent identity: annotation2094281
display id: annotation2094281
version: 1
top level: BBa_K404247
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404247/annotation2094281/range2094281/1

https://synbiohub.org/public/igem/BBa_K404247/annotation2094281/range2094281/1
type: Range
persistent identity: range2094281
display id: range2094281
version: 1
direction: direction/forward
top level: BBa_K404247
start: 671
end: 2908
orientation: inline

downstream 587
type: SequenceAnnotation
persistent identity: annotation2094287
display id: annotation2094287
version: 1
top level: BBa_K404247
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404247/annotation2094287/range2094287/1

https://synbiohub.org/public/igem/BBa_K404247/annotation2094287/range2094287/1
type: Range
persistent identity: range2094287
display id: range2094287
version: 1
direction: direction/forward
top level: BBa_K404247
start: 2462
end: 2477
orientation: inline

VP3
type: SequenceAnnotation
persistent identity: annotation2094283
display id: annotation2094283
version: 1
top level: BBa_K404247
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404247/annotation2094283/range2094283/1

https://synbiohub.org/public/igem/BBa_K404247/annotation2094283/range2094283/1
type: Range
persistent identity: range2094283
display id: range2094283
version: 1
direction: direction/forward
top level: BBa_K404247
start: 1274
end: 2908
orientation: inline

VP2
type: SequenceAnnotation
persistent identity: annotation2094282
display id: annotation2094282
version: 1
top level: BBa_K404247
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404247/annotation2094282/range2094282/1

https://synbiohub.org/public/igem/BBa_K404247/annotation2094282/range2094282/1
type: Range
persistent identity: range2094282
display id: range2094282
version: 1
direction: direction/forward
top level: BBa_K404247
start: 1079
end: 2908
orientation: inline

TG-spacer
type: SequenceAnnotation
persistent identity: annotation2094285
display id: annotation2094285
version: 1
top level: BBa_K404247
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404247/annotation2094285/range2094285/1

https://synbiohub.org/public/igem/BBa_K404247/annotation2094285/range2094285/1
type: Range
persistent identity: range2094285
display id: range2094285
version: 1
direction: direction/forward
top level: BBa_K404247
start: 2429
end: 2434
orientation: inline

upstream 587
type: SequenceAnnotation
persistent identity: annotation2094288
display id: annotation2094288
version: 1
top level: BBa_K404247
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404247/annotation2094288/range2094288/1

https://synbiohub.org/public/igem/BBa_K404247/annotation2094288/range2094288/1
type: Range
persistent identity: range2094288
display id: range2094288
version: 1
direction: direction/forward
top level: BBa_K404247
start: 2397
end: 2428
orientation: inline

CMV promoter
type: SequenceAnnotation
persistent identity: annotation2094280
display id: annotation2094280
version: 1
top level: BBa_K404247
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K404247/annotation2094280/range2094280/1

https://synbiohub.org/public/igem/BBa_K404247/annotation2094280/range2094280/1
type: Range
persistent identity: range2094280
display id: range2094280
version: 1
direction: direction/forward
top level: BBa_K404247
start: 2
end: 655
orientation: inline

His-tag
type: SequenceAnnotation
persistent identity: annotation2094284
display id: annotation2094284
version: 1
top level: BBa_K404247
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404247/annotation2094284/range2094284/1

https://synbiohub.org/public/igem/BBa_K404247/annotation2094284/range2094284/1
type: Range
persistent identity: range2094284
display id: range2094284
version: 1
direction: direction/forward
top level: BBa_K404247
start: 2435
end: 2452
orientation: inline

GLS-spacer
type: SequenceAnnotation
persistent identity: annotation2094286
display id: annotation2094286
version: 1
top level: BBa_K404247
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404247/annotation2094286/range2094286/1

https://synbiohub.org/public/igem/BBa_K404247/annotation2094286/range2094286/1
type: Range
persistent identity: range2094286
display id: range2094286
version: 1
direction: direction/forward
top level: BBa_K404247
start: 2453
end: 2461
orientation: inline

BBa_K404151
type: ComponentDefinition
persistent identity: BBa K404151
display id: BBa_K404151
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: [AAV2]-VP23 (ViralBrick-587KO-Empty)
creator: Freiburg Bioware 2010
created: 2010-10-10T11:00:00Z
modified: 2015-05-08T01:12:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 6733
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: VP2
sequence annotation: VP3
sequence annotation: AAV-2-cap (Stratagene)
sequence annotation: HSPG-KO
sequence: https://synbiohub.org/public/igem/BBa_K404151_sequence/1

VP2
type: SequenceAnnotation
persistent identity: annotation2095298
display id: annotation2095298
version: 1
top level: BBa_K404151
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404151/annotation2095298/range2095298/1

https://synbiohub.org/public/igem/BBa_K404151/annotation2095298/range2095298/1
type: Range
persistent identity: range2095298
display id: range2095298
version: 1
direction: direction/forward
top level: BBa_K404151
start: 1
end: 1794
orientation: inline

VP3
type: SequenceAnnotation
persistent identity: annotation2095299
display id: annotation2095299
version: 1
top level: BBa_K404151
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404151/annotation2095299/range2095299/1

https://synbiohub.org/public/igem/BBa_K404151/annotation2095299/range2095299/1
type: Range
persistent identity: range2095299
display id: range2095299
version: 1
direction: direction/forward
top level: BBa_K404151
start: 193
end: 1794
orientation: inline

AAV-2-cap (Stratagene)
type: SequenceAnnotation
persistent identity: annotation2095300
display id: annotation2095300
version: 1
top level: BBa_K404151
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404151/annotation2095300/range2095300/1

https://synbiohub.org/public/igem/BBa_K404151/annotation2095300/range2095300/1
type: Range
persistent identity: range2095300
display id: range2095300
version: 1
direction: direction/forward
top level: BBa_K404151
start: 1
end: 1917
orientation: inline

HSPG-KO
type: SequenceAnnotation
persistent identity: annotation2101716
display id: annotation2101716
version: 1
top level: BBa_K404151
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K404151/annotation2101716/range2101716/1

https://synbiohub.org/public/igem/BBa_K404151/annotation2101716/range2101716/1
type: Range
persistent identity: range2101716
display id: range2101716
version: 1
direction: direction/forward
top level: BBa_K404151
start: 1339
end: 1341
orientation: inline

HSPG-KO
type: SequenceAnnotation
persistent identity: annotation2101715
display id: annotation2101715
version: 1
top level: BBa_K404151
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K404151/annotation2101715/range2101715/1

https://synbiohub.org/public/igem/BBa_K404151/annotation2101715/range2101715/1
type: Range
persistent identity: range2101715
display id: range2101715
version: 1
direction: direction/forward
top level: BBa_K404151
start: 1348
end: 1350
orientation: inline

pJEJAM1
type: ComponentDefinition
persistent identity: BBa K678049
display id: BBa_K678049
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pJEJAM1
creator: DTU-Denmark-2
created: 2011-09-12T11:00:00Z
modified: 2015-05-08T01:13:04Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _882_
m user id: 0
owner id: 8341
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: to be announced
mutable notes: to be announced
mutable provenance: to be announced
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000155
role: partType/Plasmid
component: BBa_K678023
component: BBa_J52034
component: BBa_K678021
component: BBa_K678006
component: BBa_K678019
sequence annotation: BBa_K678019
sequence annotation: BBa_J52034
sequence annotation: BBa_K678006
sequence annotation: BBa_K678021
sequence annotation: BBa_K678023
sequence: https://synbiohub.org/public/igem/BBa_K678049_sequence/1

BBa_K678023
type: Component
persistent identity: component2135861
display id: component2135861
version: 1
top level: pJEJAM1
access: public
definition: https://synbiohub.org/public/igem/BBa_K678023/1

BBa_J52034
type: Component
persistent identity: component2135848
display id: component2135848
version: 1
top level: pJEJAM1
access: public
definition: https://synbiohub.org/public/igem/BBa_J52034/1

BBa_K678021
type: Component
persistent identity: component2135858
display id: component2135858
version: 1
top level: pJEJAM1
access: public
definition: hygR

BBa_K678006
type: Component
persistent identity: component2135852
display id: component2135852
version: 1
top level: pJEJAM1
access: public
definition: eGFP

BBa_K678019
type: Component
persistent identity: component2135855
display id: component2135855
version: 1
top level: pJEJAM1
access: public
definition: BGH pA

BBa_K678019
type: SequenceAnnotation
persistent identity: annotation2135855
display id: annotation2135855
version: 1
top level: pJEJAM1
location: https://synbiohub.org/public/igem/BBa_K678049/annotation2135855/range2135855/1
component: BBa_K678019

https://synbiohub.org/public/igem/BBa_K678049/annotation2135855/range2135855/1
type: Range
persistent identity: range2135855
display id: range2135855
version: 1
direction: direction/reverse
top level: pJEJAM1
start: 1390
end: 1622
orientation: inline

BBa_J52034
type: SequenceAnnotation
persistent identity: annotation2135848
display id: annotation2135848
version: 1
top level: pJEJAM1
location: https://synbiohub.org/public/igem/BBa_K678049/annotation2135848/range2135848/1
component: BBa_J52034

https://synbiohub.org/public/igem/BBa_K678049/annotation2135848/range2135848/1
type: Range
persistent identity: range2135848
display id: range2135848
version: 1
direction: direction/reverse
top level: pJEJAM1
start: 1
end: 654
orientation: inline

BBa_K678006
type: SequenceAnnotation
persistent identity: annotation2135852
display id: annotation2135852
version: 1
top level: pJEJAM1
location: https://synbiohub.org/public/igem/BBa_K678049/annotation2135852/range2135852/1
component: BBa_K678006

https://synbiohub.org/public/igem/BBa_K678049/annotation2135852/range2135852/1
type: Range
persistent identity: range2135852
display id: range2135852
version: 1
direction: direction/reverse
top level: pJEJAM1
start: 655
end: 1389
orientation: inline

BBa_K678021
type: SequenceAnnotation
persistent identity: annotation2135858
display id: annotation2135858
version: 1
top level: pJEJAM1
location: https://synbiohub.org/public/igem/BBa_K678049/annotation2135858/range2135858/1
component: BBa_K678021

https://synbiohub.org/public/igem/BBa_K678049/annotation2135858/range2135858/1
type: Range
persistent identity: range2135858
display id: range2135858
version: 1
direction: direction/reverse
top level: pJEJAM1
start: 1623
end: 3381
orientation: inline

BBa_K678023
type: SequenceAnnotation
persistent identity: annotation2135861
display id: annotation2135861
version: 1
top level: pJEJAM1
location: https://synbiohub.org/public/igem/BBa_K678049/annotation2135861/range2135861/1
component: BBa_K678023

https://synbiohub.org/public/igem/BBa_K678049/annotation2135861/range2135861/1
type: Range
persistent identity: range2135861
display id: range2135861
version: 1
direction: direction/reverse
top level: pJEJAM1
start: 3382
end: 6092
orientation: inline

BBa_K404166
type: ComponentDefinition
persistent identity: BBa K404166
display id: BBa_K404166
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pCMV_VP1up_NLS_His-Tag_[AAV2]-VP23 (ViralBrick-587KO-Empty)
creator: Freiburg Bioware 2010
created: 2010-10-10T11:00:00Z
modified: 2015-05-08T01:12:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 6733
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: VP3
sequence annotation: HSPG-KO
sequence annotation: VP2
sequence annotation: VP1up
sequence annotation: NLS
sequence annotation: AAV-2-cap (Stratagene)
sequence annotation: CMV
sequence annotation: His-tag
sequence annotation: VP1
sequence: https://synbiohub.org/public/igem/BBa_K404166_sequence/1

VP3
type: SequenceAnnotation
persistent identity: annotation2094650
display id: annotation2094650
version: 1
top level: BBa_K404166
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404166/annotation2094650/range2094650/1

https://synbiohub.org/public/igem/BBa_K404166/annotation2094650/range2094650/1
type: Range
persistent identity: range2094650
display id: range2094650
version: 1
direction: direction/forward
top level: BBa_K404166
start: 1332
end: 2933
orientation: inline

HSPG-KO
type: SequenceAnnotation
persistent identity: annotation2101830
display id: annotation2101830
version: 1
top level: BBa_K404166
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K404166/annotation2101830/range2101830/1

https://synbiohub.org/public/igem/BBa_K404166/annotation2101830/range2101830/1
type: Range
persistent identity: range2101830
display id: range2101830
version: 1
direction: direction/forward
top level: BBa_K404166
start: 2478
end: 2480
orientation: inline

VP2
type: SequenceAnnotation
persistent identity: annotation2094648
display id: annotation2094648
version: 1
top level: BBa_K404166
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404166/annotation2094648/range2094648/1

https://synbiohub.org/public/igem/BBa_K404166/annotation2094648/range2094648/1
type: Range
persistent identity: range2094648
display id: range2094648
version: 1
direction: direction/forward
top level: BBa_K404166
start: 1140
end: 2933
orientation: inline

VP1up
type: SequenceAnnotation
persistent identity: annotation2094644
display id: annotation2094644
version: 1
top level: BBa_K404166
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404166/annotation2094644/range2094644/1

https://synbiohub.org/public/igem/BBa_K404166/annotation2094644/range2094644/1
type: Range
persistent identity: range2094644
display id: range2094644
version: 1
direction: direction/forward
top level: BBa_K404166
start: 674
end: 1082
orientation: inline

NLS
type: SequenceAnnotation
persistent identity: annotation2094645
display id: annotation2094645
version: 1
top level: BBa_K404166
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404166/annotation2094645/range2094645/1

https://synbiohub.org/public/igem/BBa_K404166/annotation2094645/range2094645/1
type: Range
persistent identity: range2094645
display id: range2094645
version: 1
direction: direction/forward
top level: BBa_K404166
start: 1089
end: 1109
orientation: inline

AAV-2-cap (Stratagene)
type: SequenceAnnotation
persistent identity: annotation2094647
display id: annotation2094647
version: 1
top level: BBa_K404166
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404166/annotation2094647/range2094647/1

https://synbiohub.org/public/igem/BBa_K404166/annotation2094647/range2094647/1
type: Range
persistent identity: range2094647
display id: range2094647
version: 1
direction: direction/forward
top level: BBa_K404166
start: 1140
end: 3056
orientation: inline

CMV
type: SequenceAnnotation
persistent identity: annotation2094643
display id: annotation2094643
version: 1
top level: BBa_K404166
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K404166/annotation2094643/range2094643/1

https://synbiohub.org/public/igem/BBa_K404166/annotation2094643/range2094643/1
type: Range
persistent identity: range2094643
display id: range2094643
version: 1
direction: direction/forward
top level: BBa_K404166
start: 2
end: 655
orientation: inline

His-tag
type: SequenceAnnotation
persistent identity: annotation2094646
display id: annotation2094646
version: 1
top level: BBa_K404166
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404166/annotation2094646/range2094646/1

https://synbiohub.org/public/igem/BBa_K404166/annotation2094646/range2094646/1
type: Range
persistent identity: range2094646
display id: range2094646
version: 1
direction: direction/forward
top level: BBa_K404166
start: 1116
end: 1133
orientation: inline

VP1
type: SequenceAnnotation
persistent identity: annotation2094649
display id: annotation2094649
version: 1
top level: BBa_K404166
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404166/annotation2094649/range2094649/1

https://synbiohub.org/public/igem/BBa_K404166/annotation2094649/range2094649/1
type: Range
persistent identity: range2094649
display id: range2094649
version: 1
direction: direction/forward
top level: BBa_K404166
start: 1140
end: 2933
orientation: inline

HSPG-KO
type: SequenceAnnotation
persistent identity: annotation2101818
display id: annotation2101818
version: 1
top level: BBa_K404166
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K404166/annotation2101818/range2101818/1

https://synbiohub.org/public/igem/BBa_K404166/annotation2101818/range2101818/1
type: Range
persistent identity: range2101818
display id: range2101818
version: 1
direction: direction/forward
top level: BBa_K404166
start: 2487
end: 2489
orientation: inline

BBa_K404250
type: ComponentDefinition
persistent identity: BBa K404250
display id: BBa_K404250
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pCMV_[AAV2]-VP123ex (ViralBrick-587-Beta-lactamase)
creator: Freiburg Bioware 2010
created: 2010-10-13T11:00:00Z
modified: 2015-05-08T01:12:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 7049
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: VP1
sequence annotation: CMV promoter
sequence annotation: AAV-2-cap (Stratagene)
sequence annotation: upstream 587
sequence annotation: VP3
sequence annotation: downstream 587
sequence annotation: Beta-lactamase
sequence annotation: VP2
sequence: https://synbiohub.org/public/igem/BBa_K404250_sequence/1

VP1
type: SequenceAnnotation
persistent identity: annotation2094449
display id: annotation2094449
version: 1
top level: BBa_K404250
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404250/annotation2094449/range2094449/1

https://synbiohub.org/public/igem/BBa_K404250/annotation2094449/range2094449/1
type: Range
persistent identity: range2094449
display id: range2094449
version: 1
direction: direction/forward
top level: BBa_K404250
start: 671
end: 3664
orientation: inline

CMV promoter
type: SequenceAnnotation
persistent identity: annotation2094448
display id: annotation2094448
version: 1
top level: BBa_K404250
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K404250/annotation2094448/range2094448/1

https://synbiohub.org/public/igem/BBa_K404250/annotation2094448/range2094448/1
type: Range
persistent identity: range2094448
display id: range2094448
version: 1
direction: direction/forward
top level: BBa_K404250
start: 2
end: 655
orientation: inline

AAV-2-cap (Stratagene)
type: SequenceAnnotation
persistent identity: annotation2094506
display id: annotation2094506
version: 1
top level: BBa_K404250
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404250/annotation2094506/range2094506/1

https://synbiohub.org/public/igem/BBa_K404250/annotation2094506/range2094506/1
type: Range
persistent identity: range2094506
display id: range2094506
version: 1
direction: direction/forward
top level: BBa_K404250
start: 671
end: 3787
orientation: inline

upstream 587
type: SequenceAnnotation
persistent identity: annotation2094508
display id: annotation2094508
version: 1
top level: BBa_K404250
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404250/annotation2094508/range2094508/1

https://synbiohub.org/public/igem/BBa_K404250/annotation2094508/range2094508/1
type: Range
persistent identity: range2094508
display id: range2094508
version: 1
direction: direction/forward
top level: BBa_K404250
start: 2397
end: 2428
orientation: inline

VP3
type: SequenceAnnotation
persistent identity: annotation2094505
display id: annotation2094505
version: 1
top level: BBa_K404250
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404250/annotation2094505/range2094505/1

https://synbiohub.org/public/igem/BBa_K404250/annotation2094505/range2094505/1
type: Range
persistent identity: range2094505
display id: range2094505
version: 1
direction: direction/forward
top level: BBa_K404250
start: 1274
end: 3664
orientation: inline

downstream 587
type: SequenceAnnotation
persistent identity: annotation2094509
display id: annotation2094509
version: 1
top level: BBa_K404250
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404250/annotation2094509/range2094509/1

https://synbiohub.org/public/igem/BBa_K404250/annotation2094509/range2094509/1
type: Range
persistent identity: range2094509
display id: range2094509
version: 1
direction: direction/forward
top level: BBa_K404250
start: 3218
end: 3233
orientation: inline

Beta-lactamase
type: SequenceAnnotation
persistent identity: annotation2094507
display id: annotation2094507
version: 1
top level: BBa_K404250
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404250/annotation2094507/range2094507/1

https://synbiohub.org/public/igem/BBa_K404250/annotation2094507/range2094507/1
type: Range
persistent identity: range2094507
display id: range2094507
version: 1
direction: direction/forward
top level: BBa_K404250
start: 2429
end: 3217
orientation: inline

VP2
type: SequenceAnnotation
persistent identity: annotation2094504
display id: annotation2094504
version: 1
top level: BBa_K404250
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404250/annotation2094504/range2094504/1

https://synbiohub.org/public/igem/BBa_K404250/annotation2094504/range2094504/1
type: Range
persistent identity: range2094504
display id: range2094504
version: 1
direction: direction/forward
top level: BBa_K404250
start: 1079
end: 3664
orientation: inline

BBa_K404167
type: ComponentDefinition
persistent identity: BBa K404167
display id: BBa_K404167
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pCMV_VP1up_NLS_mVenus_VP23(ViralBrick-587-KO-BAP)
creator: Freiburg Bioware 2010
created: 2010-10-23T11:00:00Z
modified: 2015-05-08T01:12:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 6674
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: TG-spacer
sequence annotation: CMV
sequence annotation: VP1up
sequence annotation: mVenus
sequence annotation: VP3
sequence annotation: upstream 587
sequence annotation: downstream 587
sequence annotation: GLS-spacer
sequence annotation: NLS
sequence annotation: BAP
sequence annotation: HSPG-KO
sequence annotation: VP2
sequence annotation: AAV-2-cap (Stratagene)
sequence: https://synbiohub.org/public/igem/BBa_K404167_sequence/1

TG-spacer
type: SequenceAnnotation
persistent identity: annotation2095318
display id: annotation2095318
version: 1
top level: BBa_K404167
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404167/annotation2095318/range2095318/1

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type: Range
persistent identity: range2095318
display id: range2095318
version: 1
direction: direction/forward
top level: BBa_K404167
start: 3182
end: 3187
orientation: inline

CMV
type: SequenceAnnotation
persistent identity: annotation2095311
display id: annotation2095311
version: 1
top level: BBa_K404167
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K404167/annotation2095311/range2095311/1

https://synbiohub.org/public/igem/BBa_K404167/annotation2095311/range2095311/1
type: Range
persistent identity: range2095311
display id: range2095311
version: 1
direction: direction/forward
top level: BBa_K404167
start: 2
end: 655
orientation: inline

VP1up
type: SequenceAnnotation
persistent identity: annotation2095312
display id: annotation2095312
version: 1
top level: BBa_K404167
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404167/annotation2095312/range2095312/1

https://synbiohub.org/public/igem/BBa_K404167/annotation2095312/range2095312/1
type: Range
persistent identity: range2095312
display id: range2095312
version: 1
direction: direction/forward
top level: BBa_K404167
start: 673
end: 1081
orientation: inline

mVenus
type: SequenceAnnotation
persistent identity: annotation2095313
display id: annotation2095313
version: 1
top level: BBa_K404167
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404167/annotation2095313/range2095313/1

https://synbiohub.org/public/igem/BBa_K404167/annotation2095313/range2095313/1
type: Range
persistent identity: range2095313
display id: range2095313
version: 1
direction: direction/forward
top level: BBa_K404167
start: 1115
end: 1828
orientation: inline

VP3
type: SequenceAnnotation
persistent identity: annotation2095315
display id: annotation2095315
version: 1
top level: BBa_K404167
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404167/annotation2095315/range2095315/1

https://synbiohub.org/public/igem/BBa_K404167/annotation2095315/range2095315/1
type: Range
persistent identity: range2095315
display id: range2095315
version: 1
direction: direction/forward
top level: BBa_K404167
start: 2027
end: 3688
orientation: inline

upstream 587
type: SequenceAnnotation
persistent identity: annotation2095321
display id: annotation2095321
version: 1
top level: BBa_K404167
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404167/annotation2095321/range2095321/1

https://synbiohub.org/public/igem/BBa_K404167/annotation2095321/range2095321/1
type: Range
persistent identity: range2095321
display id: range2095321
version: 1
direction: direction/forward
top level: BBa_K404167
start: 3150
end: 3181
orientation: inline

downstream 587
type: SequenceAnnotation
persistent identity: annotation2095320
display id: annotation2095320
version: 1
top level: BBa_K404167
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404167/annotation2095320/range2095320/1

https://synbiohub.org/public/igem/BBa_K404167/annotation2095320/range2095320/1
type: Range
persistent identity: range2095320
display id: range2095320
version: 1
direction: direction/forward
top level: BBa_K404167
start: 3242
end: 3257
orientation: inline

GLS-spacer
type: SequenceAnnotation
persistent identity: annotation2095319
display id: annotation2095319
version: 1
top level: BBa_K404167
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404167/annotation2095319/range2095319/1

https://synbiohub.org/public/igem/BBa_K404167/annotation2095319/range2095319/1
type: Range
persistent identity: range2095319
display id: range2095319
version: 1
direction: direction/forward
top level: BBa_K404167
start: 3233
end: 3241
orientation: inline

NLS
type: SequenceAnnotation
persistent identity: annotation2095310
display id: annotation2095310
version: 1
top level: BBa_K404167
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404167/annotation2095310/range2095310/1

https://synbiohub.org/public/igem/BBa_K404167/annotation2095310/range2095310/1
type: Range
persistent identity: range2095310
display id: range2095310
version: 1
direction: direction/forward
top level: BBa_K404167
start: 1088
end: 1108
orientation: inline

BAP
type: SequenceAnnotation
persistent identity: annotation2095317
display id: annotation2095317
version: 1
top level: BBa_K404167
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404167/annotation2095317/range2095317/1

https://synbiohub.org/public/igem/BBa_K404167/annotation2095317/range2095317/1
type: Range
persistent identity: range2095317
display id: range2095317
version: 1
direction: direction/forward
top level: BBa_K404167
start: 3188
end: 3232
orientation: inline

HSPG-KO
type: SequenceAnnotation
persistent identity: annotation2101843
display id: annotation2101843
version: 1
top level: BBa_K404167
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K404167/annotation2101843/range2101843/1

https://synbiohub.org/public/igem/BBa_K404167/annotation2101843/range2101843/1
type: Range
persistent identity: range2101843
display id: range2101843
version: 1
direction: direction/forward
top level: BBa_K404167
start: 3172
end: 3174
orientation: inline

VP2
type: SequenceAnnotation
persistent identity: annotation2095314
display id: annotation2095314
version: 1
top level: BBa_K404167
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404167/annotation2095314/range2095314/1

https://synbiohub.org/public/igem/BBa_K404167/annotation2095314/range2095314/1
type: Range
persistent identity: range2095314
display id: range2095314
version: 1
direction: direction/forward
top level: BBa_K404167
start: 1835
end: 3688
orientation: inline

AAV-2-cap (Stratagene)
type: SequenceAnnotation
persistent identity: annotation2095316
display id: annotation2095316
version: 1
top level: BBa_K404167
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404167/annotation2095316/range2095316/1

https://synbiohub.org/public/igem/BBa_K404167/annotation2095316/range2095316/1
type: Range
persistent identity: range2095316
display id: range2095316
version: 1
direction: direction/forward
top level: BBa_K404167
start: 1835
end: 3811
orientation: inline

HSPG-KO
type: SequenceAnnotation
persistent identity: annotation2101842
display id: annotation2101842
version: 1
top level: BBa_K404167
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K404167/annotation2101842/range2101842/1

https://synbiohub.org/public/igem/BBa_K404167/annotation2101842/range2101842/1
type: Range
persistent identity: range2101842
display id: range2101842
version: 1
direction: direction/forward
top level: BBa_K404167
start: 3234
end: 3236
orientation: inline

BBa_K404155
type: ComponentDefinition
persistent identity: BBa K404155
display id: BBa_K404155
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pCMV_Z-EGFR-1907_Short-Linker_[AAV2]-VP23 (ViralBrick-587KO-Empty)
creator: Freiburg Bioware 2010
created: 2010-10-11T11:00:00Z
modified: 2015-05-08T01:12:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 6733
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: VP2
sequence annotation: CMV
sequence annotation: HSPG-KO
sequence annotation: VP3
sequence annotation: AAV-2-cap (Stratagene)
sequence annotation: ZEGFR_1907
sequence annotation: Short-linker
sequence: https://synbiohub.org/public/igem/BBa_K404155_sequence/1

VP2
type: SequenceAnnotation
persistent identity: annotation2094955
display id: annotation2094955
version: 1
top level: BBa_K404155
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404155/annotation2094955/range2094955/1

https://synbiohub.org/public/igem/BBa_K404155/annotation2094955/range2094955/1
type: Range
persistent identity: range2094955
display id: range2094955
version: 1
direction: direction/forward
top level: BBa_K404155
start: 869
end: 2662
orientation: inline

CMV
type: SequenceAnnotation
persistent identity: annotation2094952
display id: annotation2094952
version: 1
top level: BBa_K404155
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K404155/annotation2094952/range2094952/1

https://synbiohub.org/public/igem/BBa_K404155/annotation2094952/range2094952/1
type: Range
persistent identity: range2094952
display id: range2094952
version: 1
direction: direction/forward
top level: BBa_K404155
start: 2
end: 655
orientation: inline

HSPG-KO
type: SequenceAnnotation
persistent identity: annotation2101720
display id: annotation2101720
version: 1
top level: BBa_K404155
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K404155/annotation2101720/range2101720/1

https://synbiohub.org/public/igem/BBa_K404155/annotation2101720/range2101720/1
type: Range
persistent identity: range2101720
display id: range2101720
version: 1
direction: direction/forward
top level: BBa_K404155
start: 2216
end: 2218
orientation: inline

VP3
type: SequenceAnnotation
persistent identity: annotation2094956
display id: annotation2094956
version: 1
top level: BBa_K404155
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404155/annotation2094956/range2094956/1

https://synbiohub.org/public/igem/BBa_K404155/annotation2094956/range2094956/1
type: Range
persistent identity: range2094956
display id: range2094956
version: 1
direction: direction/forward
top level: BBa_K404155
start: 1061
end: 2662
orientation: inline

HSPG-KO
type: SequenceAnnotation
persistent identity: annotation2101721
display id: annotation2101721
version: 1
top level: BBa_K404155
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K404155/annotation2101721/range2101721/1

https://synbiohub.org/public/igem/BBa_K404155/annotation2101721/range2101721/1
type: Range
persistent identity: range2101721
display id: range2101721
version: 1
direction: direction/forward
top level: BBa_K404155
start: 2207
end: 2209
orientation: inline

AAV-2-cap (Stratagene)
type: SequenceAnnotation
persistent identity: annotation2094957
display id: annotation2094957
version: 1
top level: BBa_K404155
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404155/annotation2094957/range2094957/1

https://synbiohub.org/public/igem/BBa_K404155/annotation2094957/range2094957/1
type: Range
persistent identity: range2094957
display id: range2094957
version: 1
direction: direction/forward
top level: BBa_K404155
start: 869
end: 2785
orientation: inline

ZEGFR_1907
type: SequenceAnnotation
persistent identity: annotation2094953
display id: annotation2094953
version: 1
top level: BBa_K404155
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404155/annotation2094953/range2094953/1

https://synbiohub.org/public/igem/BBa_K404155/annotation2094953/range2094953/1
type: Range
persistent identity: range2094953
display id: range2094953
version: 1
direction: direction/forward
top level: BBa_K404155
start: 641
end: 844
orientation: inline

Short-linker
type: SequenceAnnotation
persistent identity: annotation2094954
display id: annotation2094954
version: 1
top level: BBa_K404155
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404155/annotation2094954/range2094954/1

https://synbiohub.org/public/igem/BBa_K404155/annotation2094954/range2094954/1
type: Range
persistent identity: range2094954
display id: range2094954
version: 1
direction: direction/forward
top level: BBa_K404155
start: 851
end: 862
orientation: inline

BBa_K404114
type: ComponentDefinition
persistent identity: BBa K404114
display id: BBa_K404114
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: [AAV2]-left-ITR_pCMV
creator: Freiburg Bioware 2010
created: 2010-10-10T11:00:00Z
modified: 2015-05-08T01:12:23Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 6733
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: x
mutable notes: a
mutable provenance: s
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: left ITR
sequence annotation: CMV promoter
sequence: https://synbiohub.org/public/igem/BBa_K404114_sequence/1

left ITR
type: SequenceAnnotation
persistent identity: annotation2093179
display id: annotation2093179
version: 1
top level: BBa_K404114
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404114/annotation2093179/range2093179/1

https://synbiohub.org/public/igem/BBa_K404114/annotation2093179/range2093179/1
type: Range
persistent identity: range2093179
display id: range2093179
version: 1
direction: direction/forward
top level: BBa_K404114
start: 1
end: 147
orientation: inline

CMV promoter
type: SequenceAnnotation
persistent identity: annotation2093180
display id: annotation2093180
version: 1
top level: BBa_K404114
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K404114/annotation2093180/range2093180/1

https://synbiohub.org/public/igem/BBa_K404114/annotation2093180/range2093180/1
type: Range
persistent identity: range2093180
display id: range2093180
version: 1
direction: direction/forward
top level: BBa_K404114
start: 153
end: 809
orientation: inline

BBa_K404161
type: ComponentDefinition
persistent identity: BBa K404161
display id: BBa_K404161
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pCMV_DARPin-E01_Middle-Linker_[AAV2]-VP23 (ViralBrick-587KO-Empty)
creator: Freiburg Bioware 2010
created: 2010-10-11T11:00:00Z
modified: 2015-05-08T01:12:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 6733
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: VP2
sequence annotation: DARPin-E01
sequence annotation: CMV
sequence annotation: VP3
sequence annotation: AAV-2-cap (Stratagene)
sequence annotation: middle-linker
sequence annotation: HSPG-KO
sequence: https://synbiohub.org/public/igem/BBa_K404161_sequence/1

VP2
type: SequenceAnnotation
persistent identity: annotation2095009
display id: annotation2095009
version: 1
top level: BBa_K404161
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404161/annotation2095009/range2095009/1

https://synbiohub.org/public/igem/BBa_K404161/annotation2095009/range2095009/1
type: Range
persistent identity: range2095009
display id: range2095009
version: 1
direction: direction/forward
top level: BBa_K404161
start: 1166
end: 2959
orientation: inline

DARPin-E01
type: SequenceAnnotation
persistent identity: annotation2095007
display id: annotation2095007
version: 1
top level: BBa_K404161
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404161/annotation2095007/range2095007/1

https://synbiohub.org/public/igem/BBa_K404161/annotation2095007/range2095007/1
type: Range
persistent identity: range2095007
display id: range2095007
version: 1
direction: direction/forward
top level: BBa_K404161
start: 666
end: 1130
orientation: inline

CMV
type: SequenceAnnotation
persistent identity: annotation2095006
display id: annotation2095006
version: 1
top level: BBa_K404161
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K404161/annotation2095006/range2095006/1

https://synbiohub.org/public/igem/BBa_K404161/annotation2095006/range2095006/1
type: Range
persistent identity: range2095006
display id: range2095006
version: 1
direction: direction/forward
top level: BBa_K404161
start: 2
end: 655
orientation: inline

VP3
type: SequenceAnnotation
persistent identity: annotation2095010
display id: annotation2095010
version: 1
top level: BBa_K404161
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404161/annotation2095010/range2095010/1

https://synbiohub.org/public/igem/BBa_K404161/annotation2095010/range2095010/1
type: Range
persistent identity: range2095010
display id: range2095010
version: 1
direction: direction/forward
top level: BBa_K404161
start: 1358
end: 2959
orientation: inline

AAV-2-cap (Stratagene)
type: SequenceAnnotation
persistent identity: annotation2095011
display id: annotation2095011
version: 1
top level: BBa_K404161
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404161/annotation2095011/range2095011/1

https://synbiohub.org/public/igem/BBa_K404161/annotation2095011/range2095011/1
type: Range
persistent identity: range2095011
display id: range2095011
version: 1
direction: direction/forward
top level: BBa_K404161
start: 1166
end: 3082
orientation: inline

middle-linker
type: SequenceAnnotation
persistent identity: annotation2095008
display id: annotation2095008
version: 1
top level: BBa_K404161
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404161/annotation2095008/range2095008/1

https://synbiohub.org/public/igem/BBa_K404161/annotation2095008/range2095008/1
type: Range
persistent identity: range2095008
display id: range2095008
version: 1
direction: direction/forward
top level: BBa_K404161
start: 1136
end: 1159
orientation: inline

HSPG-KO
type: SequenceAnnotation
persistent identity: annotation2101801
display id: annotation2101801
version: 1
top level: BBa_K404161
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K404161/annotation2101801/range2101801/1

https://synbiohub.org/public/igem/BBa_K404161/annotation2101801/range2101801/1
type: Range
persistent identity: range2101801
display id: range2101801
version: 1
direction: direction/forward
top level: BBa_K404161
start: 2513
end: 2515
orientation: inline

HSPG-KO
type: SequenceAnnotation
persistent identity: annotation2101802
display id: annotation2101802
version: 1
top level: BBa_K404161
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K404161/annotation2101802/range2101802/1

https://synbiohub.org/public/igem/BBa_K404161/annotation2101802/range2101802/1
type: Range
persistent identity: range2101802
display id: range2101802
version: 1
direction: direction/forward
top level: BBa_K404161
start: 2504
end: 2506
orientation: inline