Luciferase was obtained from laboratory stock. The linker was fused with a PCR.
Firefly luciferase protein. It oxidates its substrate luciferin, emitting light during the process. It can act as a reporter protein when used with a promoter. This part is made to be fused downstream to other coding sequence, so it includes the RSIAT-TEV linker in order to let the luciferase acquire the correct tertiary structure. The TEV region is the recognition site for the NiaTEV protease. The protease cuts on its site, letting the luciferase without the upstream coding sequence. The luciferase does not include ATG (Met) to initiate translation, as it needs to be fused to other coding sequence on the 5'. The translation must begin in the coding sequence fused upstream. The linker includes a random nucleotide in 5', to change the reading frame of the luciferase. That way, it will not be translated unless an indel is produced in the coding region to which the part is fused.
This part must be fused downstream to other coding sequence. In order to be used in our modular gRNA testing system, it was necessary to remove the ATG (Met codon) to avoid unexpected translation. The translation must begin in the coding sequence fused upstream. The linker includes a random nucleotide in 5', to change the reading frame of the luciferase. That way, it will not be translated unless an indel is produced in the coding region to which the part is fused.
Firefly luciferase protein. It oxidates its substrate luciferin, emitting light during the process. It can act as a reporter protein when used with a promoter. This part is made to be fused downstream to other coding sequence, so it includes the RSIAT linker in order to let the luciferase acquire the correct tertiary structure. The luciferase does not include ATG (Met) to initiate translation, as it needs to be fused to other coding sequence on the 5'. The translation must begin in the coding sequence fused upstream. The linker includes a random nucleotide in 5', to change the reading frame of the luciferase. That way, it will not be translated unless an indel is produced in the coding region to which the part is fused.
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Transcription terminator for the E.coli RNA polymerase.
Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
Firefly luciferase protein. It oxidates its substrate luciferin, emitting light during the process. It can act as a reporter protein when used with a promoter. This part is made to be fused downstream to other coding sequence, so it includes the AEK linker [A(EAAAK)3A] in order to let the luciferase acquire the correct tertiary structure. The luciferase does not include ATG (Met) to initiate translation, as it needs to be fused to other coding sequence on the 5'. The translation must begin in the coding sequence fused upstream. The linker includes a random nucleotide in 5', to change the reading frame of the luciferase. That way, it will not be translated unless an indel is produced in the coding region to which the part is fused.
Firefly luciferase protein. It oxidates its substrate luciferin, emitting light during the process. It can act as a reporter protein when used with a promoter. This part is made to be fused to the 3' with other coding sequence, so it includes the SAGTI linker in order to let the luciferase acquire the correct tertiary structure. The luciferase does not include ATG (Met) to initiate translation, as it needs to be fused to 3' of other coding sequence. The translation must begin in the coding sequence fused to the 3'. The linker includes a random nucleotide in 5', to change the reading frame of the luciferase. That way, it will not be translated unless an indel is produced in the coding region to which the part is fused.
This part must be fused to the 3' of other coding sequence. In order to be used in our modular gRNA testing system, it was necessary to remove the ATG (Met codon) to avoid unexpected translation. The translation must begin in the coding sequence fused to the 3'. The linker includes a random nucleotide in 5', to change the reading frame of the luciferase. That way, it will not be translated unless an indel is produced in the coding region to which the part is fused.
http://partsregistry.org/Part:BBa_B0030 http://partsregistry.org/Part:BBa_J23100
This part contains a constitutive promoter and strong RBS. The strength of this RBS is considered relative to BBa_B0031, BBa_B0032, BBa_B0033 and BBa_B0034. While the promotor is a member of a family of constitutive promotor parts labelled J23100 through J23119.
Bella A Okiddy, Julia Borden, Michelle Yu
RgPAL1：phenylalanine ammonia lyase (PAL) coding region
The gene RgPAL1 can express Phenylalanine ammonia lyase（PAL）, which is an??enzyme??that??catalyzes??a reaction converting L-phenylalanine??to ammonia??and??trans-cinnamic acid.PAL is the first and committed step in the??phenyl propanoid pathway??and is therefore involved in the??biosynthesis??of the??polyphenol??compounds. In our project, PAL plays an important role in L-phenylalanine???s deaminization to produce trans-cinnamic acid.
The gene RgPAL1 can express Phenylalanine ammonia lyase（PAL）.
iGEM's database and Rehmannia glutinosa Genome
Promotor-RBS:This part contains a constitutive promoter and strong RBS. The strength of this RBS is considered relative to BBa_B0031, BBa_B0032, BBa_B0033 and BBa_B0034. While the promotor is a member of a family of constitutive promotor parts labelled J23100 through J23119. RgPAL1:The gene RgPAL1 can express Phenylalanine ammonia lyase（PAL）, which is an enzyme that catalyzes a reaction converting L-phenylalanine to ammonia and trans-cinnamic acid.PAL is the first and committed step in the phenyl propanoid pathway and is therefore involved in the biosynthesis of the polyphenol compounds. In our project, PAL plays an important role in L-phenylalanine???s deaminization to produce trans-cinnamic acid.
RBS.1 (strong) -- modified from R. Weiss
Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>.
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("orig" in figure 4-14 of Ron Weiss thesis). No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info Brian Chow
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
Transcriptional terminator consisting of a 64 bp stem-loop.
Conversion of the iGEM parts registry to SBOL2.1