//chassis/eukaryote/ppatens
type: Collection
persistent identity: chassis eukaryote ppatens
display id: chassis_eukaryote_ppatens
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
owned by: james
owned by: myers
member: BBa_K1159206
member: BBa_K1159305
member: BBa_K1159003
member: BBa_K1159207
member: BBa_K1159306
member: BBa_K1159015
member: BBa_K1159315
member: BBa_K1159006
member: BBa_K1159204
member: BBa_K1159303
member: BBa_K1159010
member: BBa_K1825006
member: BBa_K1159307
member: BBa_K1159316
member: BBa_K1159001
member: BBa_K1159203
member: BBa_K1159308
member: BBa_K1825005
member: BBa_K1159120
member: BBa_K1159205
member: BBa_K1159304
member: BBa_K1159000
member: BBa_K1159314
member: BBa_K1159202
member: BBa_K1825008
member: UNIK Antif
member: BBa_K1825004

BBa_K1159314
type: ComponentDefinition
persistent identity: BBa K1159314
display id: BBa_K1159314
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TMD with N-terminal Signal Peptide of SERK Receptor from ''P. patens'' in RFC[25] N-Part
creator: Dong-Jiunn Jeffery TRUONG
created: 2013-09-15T11:00:00Z
modified: 2015-06-11T01:53:32Z
discontinued: false
dominant: false
group u list: _1471_
m user id: 4206
owner id: 11507
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This part codes for the Transmembrane Domain with N-terminal Signal Peptide of SERK Receptor from ''P. patens'' in RFC[25]. This construct is flanked by RFC[25] N-part prefix and suffix. Note: This means only protein fusions to the C-terminus of this part is possible, adding promoters (typically RFC[10]) into the prefix or terminators/IRES (typically RFC[10]) into the suffix of this part is nevertheless possible.
mutable notes: x
mutable provenance: Synthetic
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_K1159303
component: BBa_K1159993
component: BBa_K1159305
sequence annotation: BBa_K1159305
sequence annotation: BBa_K1159303
sequence annotation: BBa_K1159993
sequence: https://synbiohub.org/public/igem/BBa_K1159314_sequence/1

BBa_K1159303
type: Component
persistent identity: component2347281
display id: component2347281
version: 1
top level: BBa_K1159314
access: public
definition: BBa_K1159303

BBa_K1159993
type: Component
persistent identity: component2347283
display id: component2347283
version: 1
top level: BBa_K1159314
access: public
definition: https://synbiohub.org/public/igem/BBa_K1159993/1

BBa_K1159305
type: Component
persistent identity: component2347285
display id: component2347285
version: 1
top level: BBa_K1159314
access: public
definition: BBa_K1159305

BBa_K1159305
type: SequenceAnnotation
persistent identity: annotation2347285
display id: annotation2347285
version: 1
top level: BBa_K1159314
location: https://synbiohub.org/public/igem/BBa_K1159314/annotation2347285/range2347285/1
component: BBa_K1159305

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type: Range
persistent identity: range2347285
display id: range2347285
version: 1
direction: direction/reverse
top level: BBa_K1159314
start: 107
end: 292
orientation: inline

BBa_K1159303
type: SequenceAnnotation
persistent identity: annotation2347281
display id: annotation2347281
version: 1
top level: BBa_K1159314
location: https://synbiohub.org/public/igem/BBa_K1159314/annotation2347281/range2347281/1
component: BBa_K1159303

https://synbiohub.org/public/igem/BBa_K1159314/annotation2347281/range2347281/1
type: Range
persistent identity: range2347281
display id: range2347281
version: 1
direction: direction/reverse
top level: BBa_K1159314
start: 1
end: 100
orientation: inline

BBa_K1159993
type: SequenceAnnotation
persistent identity: annotation2347283
display id: annotation2347283
version: 1
top level: BBa_K1159314
location: https://synbiohub.org/public/igem/BBa_K1159314/annotation2347283/range2347283/1
component: BBa_K1159993

https://synbiohub.org/public/igem/BBa_K1159314/annotation2347283/range2347283/1
type: Range
persistent identity: range2347283
display id: range2347283
version: 1
direction: direction/reverse
top level: BBa_K1159314
start: 101
end: 106
orientation: inline

BBa_K1825008
type: ComponentDefinition
persistent identity: BBa K1825008
display id: BBa_K1825008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Stilbene synthase
creator: Jonathan Asmund Arnesen
created: 2015-09-11T11:00:00Z
modified: 2015-12-16T12:19:32Z
discontinued: false
dominant: false
group u list: _2251_
m user id: 4206
owner id: 28047
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This part codes for Stilbene synthase, the last enzyme in the resveratrol pathway, similarly to BBa_K1033002. However, this sequence is different and was available at the time our project started.
mutable notes: none
mutable provenance: Vitis vinifera
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1825008_sequence/1

BBa_K1159206
type: ComponentDefinition
persistent identity: BBa K1159206
display id: BBa_K1159206
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Membrane-anchored SpyCatcher in RFC[25] N-Part
creator: Dong-Jiunn Jeffery TRUONG
created: 2013-09-16T11:00:00Z
modified: 2015-06-11T01:59:18Z
discontinued: false
dominant: false
group u list: _1471_
m user id: 4206
owner id: 11507
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This part codes for a membrane-anchored version of SpyCatcher [http://parts.igem.org/wiki/index.php?title=Part:BBa_K1159200 BBa_K1159200] for the chassis ''Physcomitrella patens''. Please see subparts for for further description. This construct is flanked by RFC[25] N-part prefix and suffix. Note: This means only protein fusions to the C-terminus of this part is possible, adding promoters (typically RFC[10]) into the prefix or terminators/IRES (typically RFC[10]) into the suffix of this part is nevertheless possible.
mutable notes: x
mutable provenance: Synthetic
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_K1159993
component: BBa_K1159316
component: BBa_K1159204
sequence annotation: BBa_K1159204
sequence annotation: BBa_K1159993
sequence annotation: BBa_K1159316
sequence: https://synbiohub.org/public/igem/BBa_K1159206_sequence/1

BBa_K1159993
type: Component
persistent identity: component2348953
display id: component2348953
version: 1
top level: BBa_K1159206
access: public
definition: https://synbiohub.org/public/igem/BBa_K1159993/1

BBa_K1159316
type: Component
persistent identity: component2348970
display id: component2348970
version: 1
top level: BBa_K1159206
access: public
definition: BBa_K1159316

BBa_K1159204
type: Component
persistent identity: component2348951
display id: component2348951
version: 1
top level: BBa_K1159206
access: public
definition: BBa_K1159204

BBa_K1159204
type: SequenceAnnotation
persistent identity: annotation2348951
display id: annotation2348951
version: 1
top level: BBa_K1159206
location: https://synbiohub.org/public/igem/BBa_K1159206/annotation2348951/range2348951/1
component: BBa_K1159204

https://synbiohub.org/public/igem/BBa_K1159206/annotation2348951/range2348951/1
type: Range
persistent identity: range2348951
display id: range2348951
version: 1
direction: direction/reverse
top level: BBa_K1159206
start: 1
end: 445
orientation: inline

BBa_K1159993
type: SequenceAnnotation
persistent identity: annotation2348953
display id: annotation2348953
version: 1
top level: BBa_K1159206
location: https://synbiohub.org/public/igem/BBa_K1159206/annotation2348953/range2348953/1
component: BBa_K1159993

https://synbiohub.org/public/igem/BBa_K1159206/annotation2348953/range2348953/1
type: Range
persistent identity: range2348953
display id: range2348953
version: 1
direction: direction/reverse
top level: BBa_K1159206
start: 446
end: 451
orientation: inline

BBa_K1159316
type: SequenceAnnotation
persistent identity: annotation2348970
display id: annotation2348970
version: 1
top level: BBa_K1159206
location: https://synbiohub.org/public/igem/BBa_K1159206/annotation2348970/range2348970/1
component: BBa_K1159316

https://synbiohub.org/public/igem/BBa_K1159206/annotation2348970/range2348970/1
type: Range
persistent identity: range2348970
display id: range2348970
version: 1
direction: direction/reverse
top level: BBa_K1159206
start: 452
end: 1447
orientation: inline

BBa_K1159015
type: ComponentDefinition
persistent identity: BBa K1159015
display id: BBa_K1159015
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Membrane-anchored NanoLuc Luciferase in RFC[25] N-Part
creator: TU Munich 2013
created: 2013-05-16T11:00:00Z
modified: 2015-06-11T01:51:53Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1471_
m user id: 4206
owner id: 14732
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_K1159316
component: BBa_K1159993
component: BBa_K1159006
sequence annotation: BBa_K1159993
sequence annotation: BBa_K1159006
sequence annotation: BBa_K1159316
sequence: https://synbiohub.org/public/igem/BBa_K1159015_sequence/1

BBa_K1159316
type: Component
persistent identity: component2346513
display id: component2346513
version: 1
top level: BBa_K1159015
access: public
definition: BBa_K1159316

BBa_K1159993
type: Component
persistent identity: component2346496
display id: component2346496
version: 1
top level: BBa_K1159015
access: public
definition: https://synbiohub.org/public/igem/BBa_K1159993/1

BBa_K1159006
type: Component
persistent identity: component2346494
display id: component2346494
version: 1
top level: BBa_K1159015
access: public
definition: BBa_K1159006

BBa_K1159993
type: SequenceAnnotation
persistent identity: annotation2346496
display id: annotation2346496
version: 1
top level: BBa_K1159015
location: https://synbiohub.org/public/igem/BBa_K1159015/annotation2346496/range2346496/1
component: BBa_K1159993

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type: Range
persistent identity: range2346496
display id: range2346496
version: 1
direction: direction/reverse
top level: BBa_K1159015
start: 584
end: 589
orientation: inline

BBa_K1159006
type: SequenceAnnotation
persistent identity: annotation2346494
display id: annotation2346494
version: 1
top level: BBa_K1159015
location: https://synbiohub.org/public/igem/BBa_K1159015/annotation2346494/range2346494/1
component: BBa_K1159006

https://synbiohub.org/public/igem/BBa_K1159015/annotation2346494/range2346494/1
type: Range
persistent identity: range2346494
display id: range2346494
version: 1
direction: direction/reverse
top level: BBa_K1159015
start: 1
end: 583
orientation: inline

BBa_K1159316
type: SequenceAnnotation
persistent identity: annotation2346513
display id: annotation2346513
version: 1
top level: BBa_K1159015
location: https://synbiohub.org/public/igem/BBa_K1159015/annotation2346513/range2346513/1
component: BBa_K1159316

https://synbiohub.org/public/igem/BBa_K1159015/annotation2346513/range2346513/1
type: Range
persistent identity: range2346513
display id: range2346513
version: 1
direction: direction/reverse
top level: BBa_K1159015
start: 590
end: 1585
orientation: inline

BBa_K1159315
type: ComponentDefinition
persistent identity: BBa K1159315
display id: BBa_K1159315
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TMD of SERK Receptor from Physcomitrella patens with C-terminal GFP fusion in RFC[25]
creator: TU Munich 2013
created: 2013-09-15T11:00:00Z
modified: 2015-05-28T03:06:34Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1471_
m user id: 4206
owner id: 11507
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This part codes for the Transmembrane Domain of SERK Receptor from Physcomitrella patens'' with a C-terminal (intracellular) GFPmut1 fusion; SERK TMD and GFPmut1 are seperated by a middle long linker. Still, a signal peptide is needed for membrane integration. This part is flanked by RFC[25] pre- and suffix for further protein fusions.
mutable notes: x
mutable provenance: Synthetic
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_K243005
component: BBa_K1159311
component: BBa_K1159993
component: BBa_K1159305
sequence annotation: BBa_K1159311
sequence annotation: BBa_K1159305
sequence annotation: BBa_K243005
sequence annotation: BBa_K1159993
sequence: https://synbiohub.org/public/igem/BBa_K1159315_sequence/1

BBa_K243005
type: Component
persistent identity: component2346338
display id: component2346338
version: 1
top level: BBa_K1159315
access: public
definition: https://synbiohub.org/public/igem/BBa_K243005/1

BBa_K1159311
type: Component
persistent identity: component2346342
display id: component2346342
version: 1
top level: BBa_K1159315
access: public
definition: https://synbiohub.org/public/igem/BBa_K1159311/1

BBa_K1159993
type: Component
persistent identity: component2346336
display id: component2346336
version: 1
top level: BBa_K1159315
access: public
definition: https://synbiohub.org/public/igem/BBa_K1159993/1

BBa_K1159305
type: Component
persistent identity: component2346334
display id: component2346334
version: 1
top level: BBa_K1159315
access: public
definition: BBa_K1159305

BBa_K1159993
type: Component
persistent identity: component2346340
display id: component2346340
version: 1
top level: BBa_K1159315
access: public
definition: https://synbiohub.org/public/igem/BBa_K1159993/1

BBa_K1159311
type: SequenceAnnotation
persistent identity: annotation2346342
display id: annotation2346342
version: 1
top level: BBa_K1159315
location: https://synbiohub.org/public/igem/BBa_K1159315/annotation2346342/range2346342/1
component: BBa_K1159311

https://synbiohub.org/public/igem/BBa_K1159315/annotation2346342/range2346342/1
type: Range
persistent identity: range2346342
display id: range2346342
version: 1
direction: direction/reverse
top level: BBa_K1159315
start: 223
end: 933
orientation: inline

BBa_K1159305
type: SequenceAnnotation
persistent identity: annotation2346334
display id: annotation2346334
version: 1
top level: BBa_K1159315
location: https://synbiohub.org/public/igem/BBa_K1159315/annotation2346334/range2346334/1
component: BBa_K1159305

https://synbiohub.org/public/igem/BBa_K1159315/annotation2346334/range2346334/1
type: Range
persistent identity: range2346334
display id: range2346334
version: 1
direction: direction/reverse
top level: BBa_K1159315
start: 1
end: 186
orientation: inline

BBa_K243005
type: SequenceAnnotation
persistent identity: annotation2346338
display id: annotation2346338
version: 1
top level: BBa_K1159315
location: https://synbiohub.org/public/igem/BBa_K1159315/annotation2346338/range2346338/1
component: BBa_K243005

https://synbiohub.org/public/igem/BBa_K1159315/annotation2346338/range2346338/1
type: Range
persistent identity: range2346338
display id: range2346338
version: 1
direction: direction/reverse
top level: BBa_K1159315
start: 193
end: 216
orientation: inline

BBa_K1159993
type: SequenceAnnotation
persistent identity: annotation2346336
display id: annotation2346336
version: 1
top level: BBa_K1159315
location: https://synbiohub.org/public/igem/BBa_K1159315/annotation2346336/range2346336/1
component: BBa_K1159993

https://synbiohub.org/public/igem/BBa_K1159315/annotation2346336/range2346336/1
type: Range
persistent identity: range2346336
display id: range2346336
version: 1
direction: direction/reverse
top level: BBa_K1159315
start: 187
end: 192
orientation: inline

BBa_K1159993
type: SequenceAnnotation
persistent identity: annotation2346340
display id: annotation2346340
version: 1
top level: BBa_K1159315
location: https://synbiohub.org/public/igem/BBa_K1159315/annotation2346340/range2346340/1
component: BBa_K1159993

https://synbiohub.org/public/igem/BBa_K1159315/annotation2346340/range2346340/1
type: Range
persistent identity: range2346340
display id: range2346340
version: 1
direction: direction/reverse
top level: BBa_K1159315
start: 217
end: 222
orientation: inline

BBa_K1159305
type: ComponentDefinition
persistent identity: BBa K1159305
display id: BBa_K1159305
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Transmembrane domain of SERK Receptor from Physcomitrella patens in RFC[25]
creator: TU Munich 2013
created: 2013-09-13T11:00:00Z
modified: 2015-06-11T01:54:11Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1471_
m user id: 4206
owner id: 11507
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This part is encoding the transmembrane region of the SERK Receptor from ''Physcomitrella patens''. For membrane translocation a N-terminal signal peptide is still necessary for translocation through the ER membrane. This part is flanked by RFC[25] pre- and suffix.
mutable notes: x
mutable provenance: ''Physcomitrella patens''
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: Transmembrane Domain of SERK Receptor from ''P. patens''
sequence: https://synbiohub.org/public/igem/BBa_K1159305_sequence/1

Transmembrane Domain of SERK Receptor from ''P. patens''
type: SequenceAnnotation
persistent identity: annotation2343588
display id: annotation2343588
version: 1
top level: BBa_K1159305
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1159305/annotation2343588/range2343588/1

https://synbiohub.org/public/igem/BBa_K1159305/annotation2343588/range2343588/1
type: Range
persistent identity: range2343588
display id: range2343588
version: 1
direction: direction/forward
top level: BBa_K1159305
start: 1
end: 186
orientation: inline

BBa_K1159003
type: ComponentDefinition
persistent identity: BBa K1159003
display id: BBa_K1159003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Engineered Fluorescein-Binding Anticalin FluA (triple mutant variant) in RFC[25]
creator: TU Munich 2013
created: 2013-05-16T11:00:00Z
modified: 2015-05-28T03:07:12Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1471_
m user id: 4206
owner id: 14732
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Arg --> Lys
sequence annotation: FluA triple mutant
sequence annotation: Ser --> Thr
sequence annotation: Ala --> Ile
sequence: https://synbiohub.org/public/igem/BBa_K1159003_sequence/1

Arg --> Lys
type: SequenceAnnotation
persistent identity: annotation2343157
display id: annotation2343157
version: 1
top level: BBa_K1159003
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K1159003/annotation2343157/range2343157/1

https://synbiohub.org/public/igem/BBa_K1159003/annotation2343157/range2343157/1
type: Range
persistent identity: range2343157
display id: range2343157
version: 1
direction: direction/forward
top level: BBa_K1159003
start: 283
end: 285
orientation: inline

FluA triple mutant
type: SequenceAnnotation
persistent identity: annotation2343011
display id: annotation2343011
version: 1
top level: BBa_K1159003
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1159003/annotation2343011/range2343011/1

https://synbiohub.org/public/igem/BBa_K1159003/annotation2343011/range2343011/1
type: Range
persistent identity: range2343011
display id: range2343011
version: 1
direction: direction/forward
top level: BBa_K1159003
start: 1
end: 522
orientation: inline

Ser --> Thr
type: SequenceAnnotation
persistent identity: annotation2343158
display id: annotation2343158
version: 1
top level: BBa_K1159003
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K1159003/annotation2343158/range2343158/1

https://synbiohub.org/public/igem/BBa_K1159003/annotation2343158/range2343158/1
type: Range
persistent identity: range2343158
display id: range2343158
version: 1
direction: direction/forward
top level: BBa_K1159003
start: 340
end: 342
orientation: inline

Ala --> Ile
type: SequenceAnnotation
persistent identity: annotation2343143
display id: annotation2343143
version: 1
top level: BBa_K1159003
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K1159003/annotation2343143/range2343143/1

https://synbiohub.org/public/igem/BBa_K1159003/annotation2343143/range2343143/1
type: Range
persistent identity: range2343143
display id: range2343143
version: 1
direction: direction/forward
top level: BBa_K1159003
start: 133
end: 135
orientation: inline

BBa_K1159001
type: ComponentDefinition
persistent identity: BBa K1159001
display id: BBa_K1159001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: NanoLuc Luciferase in RFC[25]
creator: TU Munich 2013
created: 2013-05-16T11:00:00Z
modified: 2016-01-26T02:12:53Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1471_
m user id: 4206
owner id: 14732
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: NanoLuc Luciferase
sequence: https://synbiohub.org/public/igem/BBa_K1159001_sequence/1

NanoLuc Luciferase
type: SequenceAnnotation
persistent identity: annotation2342942
display id: annotation2342942
version: 1
top level: BBa_K1159001
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1159001/annotation2342942/range2342942/1

https://synbiohub.org/public/igem/BBa_K1159001/annotation2342942/range2342942/1
type: Range
persistent identity: range2342942
display id: range2342942
version: 1
direction: direction/forward
top level: BBa_K1159001
start: 1
end: 510
orientation: inline

BBa_K1159000
type: ComponentDefinition
persistent identity: BBa K1159000
display id: BBa_K1159000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Erythromycin Esterase Type II (EreB) in RFC[25]
creator: Louise FUNKE
created: 2013-05-16T11:00:00Z
modified: 2015-05-28T02:59:15Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1471_
m user id: 4206
owner id: 14732
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Erythromycin Esterase Type II
sequence: https://synbiohub.org/public/igem/BBa_K1159000_sequence/1

Erythromycin Esterase Type II
type: SequenceAnnotation
persistent identity: annotation2342950
display id: annotation2342950
version: 1
top level: BBa_K1159000
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1159000/annotation2342950/range2342950/1

https://synbiohub.org/public/igem/BBa_K1159000/annotation2342950/range2342950/1
type: Range
persistent identity: range2342950
display id: range2342950
version: 1
direction: direction/forward
top level: BBa_K1159000
start: 1
end: 1254
orientation: inline

BBa_K1159202
type: ComponentDefinition
persistent identity: BBa K1159202
display id: BBa_K1159202
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Secretory SpyCatcher (IgKappa-SigP_SpyCatcher) in RFC[25] N-Part
creator: Dong-Jiunn Jeffery TRUONG
created: 2013-09-15T11:00:00Z
modified: 2015-06-11T02:00:29Z
discontinued: false
dominant: false
group u list: _1471_
m user id: 4206
owner id: 11507
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This part encodes the SpyCatcher [http://parts.igem.org/Part:BBa_K1159200 BBa_K1159200] with a N-terminal signal peptide for secretion (Ig Kappa signal peptide from ''Mus musculus'') which also works in moss ''Physcomitrella patens''. This construct is flanked by RFC[25] N-part prefix and suffix. Note: This means only protein fusions to the C-terminus of this part is possible, adding promoters (typically RFC[10]) into the prefix or terminators/IRES (typically RFC[10]) into the suffix of this part is nevertheless possible.
mutable notes: x
mutable provenance: Synthetic
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_K1159993
component: BBa_K1159304
component: BBa_K1159200
sequence annotation: BBa_K1159993
sequence annotation: BBa_K1159304
sequence annotation: BBa_K1159200
sequence: https://synbiohub.org/public/igem/BBa_K1159202_sequence/1

BBa_K1159993
type: Component
persistent identity: component2348478
display id: component2348478
version: 1
top level: BBa_K1159202
access: public
definition: https://synbiohub.org/public/igem/BBa_K1159993/1

BBa_K1159304
type: Component
persistent identity: component2348476
display id: component2348476
version: 1
top level: BBa_K1159202
access: public
definition: BBa_K1159304

BBa_K1159200
type: Component
persistent identity: component2348483
display id: component2348483
version: 1
top level: BBa_K1159202
access: public
definition: https://synbiohub.org/public/igem/BBa_K1159200/1

BBa_K1159993
type: SequenceAnnotation
persistent identity: annotation2348478
display id: annotation2348478
version: 1
top level: BBa_K1159202
location: https://synbiohub.org/public/igem/BBa_K1159202/annotation2348478/range2348478/1
component: BBa_K1159993

https://synbiohub.org/public/igem/BBa_K1159202/annotation2348478/range2348478/1
type: Range
persistent identity: range2348478
display id: range2348478
version: 1
direction: direction/reverse
top level: BBa_K1159202
start: 68
end: 73
orientation: inline

BBa_K1159304
type: SequenceAnnotation
persistent identity: annotation2348476
display id: annotation2348476
version: 1
top level: BBa_K1159202
location: https://synbiohub.org/public/igem/BBa_K1159202/annotation2348476/range2348476/1
component: BBa_K1159304

https://synbiohub.org/public/igem/BBa_K1159202/annotation2348476/range2348476/1
type: Range
persistent identity: range2348476
display id: range2348476
version: 1
direction: direction/reverse
top level: BBa_K1159202
start: 1
end: 67
orientation: inline

BBa_K1159200
type: SequenceAnnotation
persistent identity: annotation2348483
display id: annotation2348483
version: 1
top level: BBa_K1159202
location: https://synbiohub.org/public/igem/BBa_K1159202/annotation2348483/range2348483/1
component: BBa_K1159200

https://synbiohub.org/public/igem/BBa_K1159202/annotation2348483/range2348483/1
type: Range
persistent identity: range2348483
display id: range2348483
version: 1
direction: direction/reverse
top level: BBa_K1159202
start: 74
end: 412
orientation: inline

BBa_K1825005
type: ComponentDefinition
persistent identity: BBa K1825005
display id: BBa_K1825005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: nptII
creator: Jonathan Asmund Arnesen
created: 2015-09-02T11:00:00Z
modified: 2015-12-16T12:17:11Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2251_
m user id: 4206
owner id: 28047
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Neomycin Phosphotransferase, for a composite biobrick.
mutable notes: none
mutable provenance: From a vector.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1825005_sequence/1

BBa_K1159307
type: ComponentDefinition
persistent identity: BBa K1159307
display id: BBa_K1159307
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: 35S Terminator of Cauliflower Mosaic Virus (CaMV)
creator: TU Munich 2013
created: 2013-09-13T11:00:00Z
modified: 2015-06-11T01:46:04Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1471_
m user id: 4206
owner id: 11507
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This part contains the t35S terminator of the Cauliflower Mosaic Virus (CaMV), a plant virus. This part is flanked by RFC[10] pre- and suffix.
mutable notes: x
mutable provenance: Cauliflower Mosaic Virus
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: CaMV 35S Terminator
sequence: https://synbiohub.org/public/igem/BBa_K1159307_sequence/1

CaMV 35S Terminator
type: SequenceAnnotation
persistent identity: annotation2341614
display id: annotation2341614
version: 1
top level: BBa_K1159307
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1159307/annotation2341614/range2341614/1

https://synbiohub.org/public/igem/BBa_K1159307/annotation2341614/range2341614/1
type: Range
persistent identity: range2341614
display id: range2341614
version: 1
direction: direction/forward
top level: BBa_K1159307
start: 1
end: 217
orientation: inline

BBa_K1159304
type: ComponentDefinition
persistent identity: BBa K1159304
display id: BBa_K1159304
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Signal Peptide of Ig Kappa chain from Mus musculus in RFC[25] N-part
creator: TU Munich 2013
created: 2013-09-13T11:00:00Z
modified: 2015-06-11T01:54:39Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1471_
m user id: 4206
owner id: 11507
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This part codes for the signal peptide of the Ig Kappa chain from ''Mus musculus''. The signal peptide induces translocation of the protein into the Endoplasmatic Reticulum (ER) for secretion or membrane integration. Protein fused to the C-terminus of this part should be secreted or otherwise if the part fused region contains a transmembrane domain it will be integrated in cytoplasmatic membrane. This part starts with a consensus sequence for Physcomitrella patens which allows efficient translation start. Furthermore, this part is flanked by RFC[25] N-part pre- and suffix for fusions to the C-terminus of this part.
mutable notes: x
mutable provenance: ''Mus musculus''
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: Ig Kappa Signal Peptide
sequence annotation: Consensus Sequence for P. patens
sequence: https://synbiohub.org/public/igem/BBa_K1159304_sequence/1

Ig Kappa Signal Peptide
type: SequenceAnnotation
persistent identity: annotation2341499
display id: annotation2341499
version: 1
top level: BBa_K1159304
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1159304/annotation2341499/range2341499/1

https://synbiohub.org/public/igem/BBa_K1159304/annotation2341499/range2341499/1
type: Range
persistent identity: range2341499
display id: range2341499
version: 1
direction: direction/forward
top level: BBa_K1159304
start: 5
end: 67
orientation: inline

Consensus Sequence for P. patens
type: SequenceAnnotation
persistent identity: annotation2341498
display id: annotation2341498
version: 1
top level: BBa_K1159304
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1159304/annotation2341498/range2341498/1

https://synbiohub.org/public/igem/BBa_K1159304/annotation2341498/range2341498/1
type: Range
persistent identity: range2341498
display id: range2341498
version: 1
direction: direction/forward
top level: BBa_K1159304
start: 1
end: 4
orientation: inline

BBa_K1159207
type: ComponentDefinition
persistent identity: BBa K1159207
display id: BBa_K1159207
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Membrane-anchored SpyTag in RFC[25] N-Part
creator: Dong-Jiunn Jeffery TRUONG
created: 2013-09-16T11:00:00Z
modified: 2015-06-11T01:58:39Z
discontinued: false
dominant: false
group u list: _1471_
m user id: 4206
owner id: 11507
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This part codes for a membrane-anchored version of SpyTag [http://parts.igem.org/wiki/index.php?title=Part:BBa_K1159201 BBa_K1159201] for the chassis ''Physcomitrella patens''. Please see subparts for for further description. This construct is flanked by RFC[25] N-part prefix and suffix. Note: This means only protein fusions to the C-terminus of this part is possible, adding promoters (typically RFC[10]) into the prefix or terminators/IRES (typically RFC[10]) into the suffix of this part is nevertheless possible.
mutable notes: x
mutable provenance: Synthetic
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_K1159993
component: BBa_K1159316
component: BBa_K1159205
sequence annotation: BBa_K1159205
sequence annotation: BBa_K1159316
sequence annotation: BBa_K1159993
sequence: https://synbiohub.org/public/igem/BBa_K1159207_sequence/1

BBa_K1159993
type: Component
persistent identity: component2348986
display id: component2348986
version: 1
top level: BBa_K1159207
access: public
definition: https://synbiohub.org/public/igem/BBa_K1159993/1

BBa_K1159316
type: Component
persistent identity: component2349003
display id: component2349003
version: 1
top level: BBa_K1159207
access: public
definition: BBa_K1159316

BBa_K1159205
type: Component
persistent identity: component2348984
display id: component2348984
version: 1
top level: BBa_K1159207
access: public
definition: BBa_K1159205

BBa_K1159205
type: SequenceAnnotation
persistent identity: annotation2348984
display id: annotation2348984
version: 1
top level: BBa_K1159207
location: https://synbiohub.org/public/igem/BBa_K1159207/annotation2348984/range2348984/1
component: BBa_K1159205

https://synbiohub.org/public/igem/BBa_K1159207/annotation2348984/range2348984/1
type: Range
persistent identity: range2348984
display id: range2348984
version: 1
direction: direction/reverse
top level: BBa_K1159207
start: 1
end: 145
orientation: inline

BBa_K1159316
type: SequenceAnnotation
persistent identity: annotation2349003
display id: annotation2349003
version: 1
top level: BBa_K1159207
location: https://synbiohub.org/public/igem/BBa_K1159207/annotation2349003/range2349003/1
component: BBa_K1159316

https://synbiohub.org/public/igem/BBa_K1159207/annotation2349003/range2349003/1
type: Range
persistent identity: range2349003
display id: range2349003
version: 1
direction: direction/reverse
top level: BBa_K1159207
start: 152
end: 1147
orientation: inline

BBa_K1159993
type: SequenceAnnotation
persistent identity: annotation2348986
display id: annotation2348986
version: 1
top level: BBa_K1159207
location: https://synbiohub.org/public/igem/BBa_K1159207/annotation2348986/range2348986/1
component: BBa_K1159993

https://synbiohub.org/public/igem/BBa_K1159207/annotation2348986/range2348986/1
type: Range
persistent identity: range2348986
display id: range2348986
version: 1
direction: direction/reverse
top level: BBa_K1159207
start: 146
end: 151
orientation: inline

UNIK Antif
type: ComponentDefinition
persistent identity: BBa K1825000
display id: BBa_K1825000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: UNIK antifreeze protein
creator: Adam Petersen
created: 2015-08-30T11:00:00Z
modified: 2016-01-27T10:30:03Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2251_
m user id: 4206
owner id: 28032
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This is the coding region of a small antifreeze protein. It works by binding to the ice crystals as they form inside the cell, and by doing so prevents the ice crystals from enlarging and penetrating the cell membrane. We wanted to test if there would be a significant difference in the growth rate of moss, Physcomitrella patens, with the antifreeze gene, compared to the one without. This would be done at a low temperature to test the in-vivo function of the antifreeze protein.
mutable notes: We used codon optimization for moss so it would work better in Physcomitrella Patens.
mutable provenance: The part is taken from a spruce budworm, Choristoneura fumiferana, living in areas with permafrost.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: startcodon
sequence: https://synbiohub.org/public/igem/BBa_K1825000_sequence/1

startcodon
type: SequenceAnnotation
persistent identity: annotation2440844
display id: annotation2440844
version: 1
top level: UNIK Antif
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1825000/annotation2440844/range2440844/1

https://synbiohub.org/public/igem/BBa_K1825000/annotation2440844/range2440844/1
type: Range
persistent identity: range2440844
display id: range2440844
version: 1
direction: direction/forward
top level: UNIK Antif
start: 1
end: 3
orientation: inline

BBa_K1159006
type: ComponentDefinition
persistent identity: BBa K1159006
display id: BBa_K1159006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Secretory NanoLuc Luciferase (IgKappa-SigP_nLuc) in RFC[25] N-Part
creator: TU Munich 2013
created: 2013-09-14T11:00:00Z
modified: 2015-05-28T03:52:04Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1471_
m user id: 4206
owner id: 11507
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: NanoLuc Luciferase is engineered ATP-independent luciferase from a deep-sea shrimp which luminescense 2 magnitues higher than these from Renilla reniformis or from Photinus pyralis (firefly). Also the molecular weight of NanoLuc luciferase is twice smaller compared to other luciferase (only 19 kDa). This part encodes for a NanoLuc Luciferase which is fused to a N-terminal signal peptide for secretion (IgKappa signal peptide from Mus musculus) with also works in moss (Physcomitrella patens). This construct is flanked by RFC[25] N-part prefix and suffix. Note: This means only protein fusions to the C-terminus of this part is possible, adding promoters (typically RFC[10]) into the prefix or terminators/IRES (typically RFC[10]) into the suffix of this part is nevertheless possible.
mutable notes: x
mutable provenance: Synthetic
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_K1159304
component: BBa_K1159001
component: BBa_K1159993
sequence annotation: BBa_K1159993
sequence annotation: BBa_K1159304
sequence annotation: BBa_K1159001
sequence: https://synbiohub.org/public/igem/BBa_K1159006_sequence/1

BBa_K1159304
type: Component
persistent identity: component2343006
display id: component2343006
version: 1
top level: BBa_K1159006
access: public
definition: BBa_K1159304

BBa_K1159001
type: Component
persistent identity: component2343010
display id: component2343010
version: 1
top level: BBa_K1159006
access: public
definition: BBa_K1159001

BBa_K1159993
type: Component
persistent identity: component2343008
display id: component2343008
version: 1
top level: BBa_K1159006
access: public
definition: https://synbiohub.org/public/igem/BBa_K1159993/1

BBa_K1159993
type: SequenceAnnotation
persistent identity: annotation2343008
display id: annotation2343008
version: 1
top level: BBa_K1159006
location: https://synbiohub.org/public/igem/BBa_K1159006/annotation2343008/range2343008/1
component: BBa_K1159993

https://synbiohub.org/public/igem/BBa_K1159006/annotation2343008/range2343008/1
type: Range
persistent identity: range2343008
display id: range2343008
version: 1
direction: direction/reverse
top level: BBa_K1159006
start: 68
end: 73
orientation: inline

BBa_K1159304
type: SequenceAnnotation
persistent identity: annotation2343006
display id: annotation2343006
version: 1
top level: BBa_K1159006
location: https://synbiohub.org/public/igem/BBa_K1159006/annotation2343006/range2343006/1
component: BBa_K1159304

https://synbiohub.org/public/igem/BBa_K1159006/annotation2343006/range2343006/1
type: Range
persistent identity: range2343006
display id: range2343006
version: 1
direction: direction/reverse
top level: BBa_K1159006
start: 1
end: 67
orientation: inline

BBa_K1159001
type: SequenceAnnotation
persistent identity: annotation2343010
display id: annotation2343010
version: 1
top level: BBa_K1159006
location: https://synbiohub.org/public/igem/BBa_K1159006/annotation2343010/range2343010/1
component: BBa_K1159001

https://synbiohub.org/public/igem/BBa_K1159006/annotation2343010/range2343010/1
type: Range
persistent identity: range2343010
display id: range2343010
version: 1
direction: direction/reverse
top level: BBa_K1159006
start: 74
end: 583
orientation: inline

BBa_K1825004
type: ComponentDefinition
persistent identity: BBa K1825004
display id: BBa_K1825004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: 35s CaMV
creator: Jonathan Asmund Arnesen
created: 2015-09-02T11:00:00Z
modified: 2015-12-16T12:16:03Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2251_
m user id: 4206
owner id: 28047
owning group id: 9
status: status/Planning
bookmark: false
mutable description: 35s Cauliflower mosaic virus promoter, for a composite part
mutable notes: none
mutable provenance: Cauliflower mosaic virus
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K1825004_sequence/1

BBa_K1159204
type: ComponentDefinition
persistent identity: BBa K1159204
display id: BBa_K1159204
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Secretory SpyCatcher (SERK-SigP_SpyCatcher) in RFC[25] N-Part
creator: Dong-Jiunn Jeffery TRUONG
created: 2013-09-16T11:00:00Z
modified: 2015-06-11T01:59:58Z
discontinued: false
dominant: false
group u list: _1471_
m user id: 4206
owner id: 11507
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This part encodes the SpyCatcher [http://parts.igem.org/Part:BBa_K1159200 BBa_K1159200] with a N-terminal signal peptide for secretion (SERK receptor signal peptide from ''Physcomitrella patens''). This construct is flanked by RFC[25] N-part prefix and suffix. Note: This means only protein fusions to the C-terminus of this part is possible, adding promoters (typically RFC[10]) into the prefix or terminators/IRES (typically RFC[10]) into the suffix of this part is nevertheless possible.
mutable notes: x
mutable provenance: Synthetic
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_K1159303
component: BBa_K1159200
component: BBa_K1159993
sequence annotation: BBa_K1159200
sequence annotation: BBa_K1159993
sequence annotation: BBa_K1159303
sequence: https://synbiohub.org/public/igem/BBa_K1159204_sequence/1

BBa_K1159303
type: Component
persistent identity: component2348879
display id: component2348879
version: 1
top level: BBa_K1159204
access: public
definition: BBa_K1159303

BBa_K1159200
type: Component
persistent identity: component2348886
display id: component2348886
version: 1
top level: BBa_K1159204
access: public
definition: https://synbiohub.org/public/igem/BBa_K1159200/1

BBa_K1159993
type: Component
persistent identity: component2348881
display id: component2348881
version: 1
top level: BBa_K1159204
access: public
definition: https://synbiohub.org/public/igem/BBa_K1159993/1

BBa_K1159200
type: SequenceAnnotation
persistent identity: annotation2348886
display id: annotation2348886
version: 1
top level: BBa_K1159204
location: https://synbiohub.org/public/igem/BBa_K1159204/annotation2348886/range2348886/1
component: BBa_K1159200

https://synbiohub.org/public/igem/BBa_K1159204/annotation2348886/range2348886/1
type: Range
persistent identity: range2348886
display id: range2348886
version: 1
direction: direction/reverse
top level: BBa_K1159204
start: 107
end: 445
orientation: inline

BBa_K1159993
type: SequenceAnnotation
persistent identity: annotation2348881
display id: annotation2348881
version: 1
top level: BBa_K1159204
location: https://synbiohub.org/public/igem/BBa_K1159204/annotation2348881/range2348881/1
component: BBa_K1159993

https://synbiohub.org/public/igem/BBa_K1159204/annotation2348881/range2348881/1
type: Range
persistent identity: range2348881
display id: range2348881
version: 1
direction: direction/reverse
top level: BBa_K1159204
start: 101
end: 106
orientation: inline

BBa_K1159303
type: SequenceAnnotation
persistent identity: annotation2348879
display id: annotation2348879
version: 1
top level: BBa_K1159204
location: https://synbiohub.org/public/igem/BBa_K1159204/annotation2348879/range2348879/1
component: BBa_K1159303

https://synbiohub.org/public/igem/BBa_K1159204/annotation2348879/range2348879/1
type: Range
persistent identity: range2348879
display id: range2348879
version: 1
direction: direction/reverse
top level: BBa_K1159204
start: 1
end: 100
orientation: inline

BBa_K1159010
type: ComponentDefinition
persistent identity: BBa K1159010
display id: BBa_K1159010
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Secretory NanoLuc Luciferase (SERK-SigP_nLuc) in RFC[25] N-Part
creator: TU Munich 2013
created: 2013-05-16T11:00:00Z
modified: 2015-05-28T03:51:17Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1471_
m user id: 4206
owner id: 14732
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_K1159303
component: BBa_K1159993
component: BBa_K1159001
sequence annotation: BBa_K1159001
sequence annotation: BBa_K1159993
sequence annotation: BBa_K1159303
sequence: https://synbiohub.org/public/igem/BBa_K1159010_sequence/1

BBa_K1159303
type: Component
persistent identity: component2343638
display id: component2343638
version: 1
top level: BBa_K1159010
access: public
definition: BBa_K1159303

BBa_K1159993
type: Component
persistent identity: component2343640
display id: component2343640
version: 1
top level: BBa_K1159010
access: public
definition: https://synbiohub.org/public/igem/BBa_K1159993/1

BBa_K1159001
type: Component
persistent identity: component2343642
display id: component2343642
version: 1
top level: BBa_K1159010
access: public
definition: BBa_K1159001

BBa_K1159001
type: SequenceAnnotation
persistent identity: annotation2343642
display id: annotation2343642
version: 1
top level: BBa_K1159010
location: https://synbiohub.org/public/igem/BBa_K1159010/annotation2343642/range2343642/1
component: BBa_K1159001

https://synbiohub.org/public/igem/BBa_K1159010/annotation2343642/range2343642/1
type: Range
persistent identity: range2343642
display id: range2343642
version: 1
direction: direction/reverse
top level: BBa_K1159010
start: 107
end: 616
orientation: inline

BBa_K1159993
type: SequenceAnnotation
persistent identity: annotation2343640
display id: annotation2343640
version: 1
top level: BBa_K1159010
location: https://synbiohub.org/public/igem/BBa_K1159010/annotation2343640/range2343640/1
component: BBa_K1159993

https://synbiohub.org/public/igem/BBa_K1159010/annotation2343640/range2343640/1
type: Range
persistent identity: range2343640
display id: range2343640
version: 1
direction: direction/reverse
top level: BBa_K1159010
start: 101
end: 106
orientation: inline

BBa_K1159303
type: SequenceAnnotation
persistent identity: annotation2343638
display id: annotation2343638
version: 1
top level: BBa_K1159010
location: https://synbiohub.org/public/igem/BBa_K1159010/annotation2343638/range2343638/1
component: BBa_K1159303

https://synbiohub.org/public/igem/BBa_K1159010/annotation2343638/range2343638/1
type: Range
persistent identity: range2343638
display id: range2343638
version: 1
direction: direction/reverse
top level: BBa_K1159010
start: 1
end: 100
orientation: inline

BBa_K1159203
type: ComponentDefinition
persistent identity: BBa K1159203
display id: BBa_K1159203
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Secretory SpyTag (IgKappa-SigP_SpyTag) in RFC[25] N-Part
creator: Dong-Jiunn Jeffery TRUONG
created: 2013-09-16T11:00:00Z
modified: 2015-06-11T02:00:20Z
discontinued: false
dominant: false
group u list: _1471_
m user id: 4206
owner id: 11507
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This part encodes the SpyTag [http://parts.igem.org/Part:BBa_K1159201 BBa_K1159201] with a N-terminal signal peptide for secretion (Ig Kappa signal peptide from ''Mus musculus'') which also works in moss ''Physcomitrella patens''. This construct is flanked by RFC[25] N-part prefix and suffix. Note: This means only protein fusions to the C-terminus of this part is possible, adding promoters (typically RFC[10]) into the prefix or terminators/IRES (typically RFC[10]) into the suffix of this part is nevertheless possible.
mutable notes: x
mutable provenance: Synthetic
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_K1159993
component: BBa_K1159304
component: BBa_K1159201
sequence annotation: BBa_K1159201
sequence annotation: BBa_K1159993
sequence annotation: BBa_K1159304
sequence: https://synbiohub.org/public/igem/BBa_K1159203_sequence/1

BBa_K1159993
type: Component
persistent identity: component2348823
display id: component2348823
version: 1
top level: BBa_K1159203
access: public
definition: https://synbiohub.org/public/igem/BBa_K1159993/1

BBa_K1159304
type: Component
persistent identity: component2348821
display id: component2348821
version: 1
top level: BBa_K1159203
access: public
definition: BBa_K1159304

BBa_K1159201
type: Component
persistent identity: component2348826
display id: component2348826
version: 1
top level: BBa_K1159203
access: public
definition: https://synbiohub.org/public/igem/BBa_K1159201/1

BBa_K1159201
type: SequenceAnnotation
persistent identity: annotation2348826
display id: annotation2348826
version: 1
top level: BBa_K1159203
location: https://synbiohub.org/public/igem/BBa_K1159203/annotation2348826/range2348826/1
component: BBa_K1159201

https://synbiohub.org/public/igem/BBa_K1159203/annotation2348826/range2348826/1
type: Range
persistent identity: range2348826
display id: range2348826
version: 1
direction: direction/reverse
top level: BBa_K1159203
start: 74
end: 112
orientation: inline

BBa_K1159993
type: SequenceAnnotation
persistent identity: annotation2348823
display id: annotation2348823
version: 1
top level: BBa_K1159203
location: https://synbiohub.org/public/igem/BBa_K1159203/annotation2348823/range2348823/1
component: BBa_K1159993

https://synbiohub.org/public/igem/BBa_K1159203/annotation2348823/range2348823/1
type: Range
persistent identity: range2348823
display id: range2348823
version: 1
direction: direction/reverse
top level: BBa_K1159203
start: 68
end: 73
orientation: inline

BBa_K1159304
type: SequenceAnnotation
persistent identity: annotation2348821
display id: annotation2348821
version: 1
top level: BBa_K1159203
location: https://synbiohub.org/public/igem/BBa_K1159203/annotation2348821/range2348821/1
component: BBa_K1159304

https://synbiohub.org/public/igem/BBa_K1159203/annotation2348821/range2348821/1
type: Range
persistent identity: range2348821
display id: range2348821
version: 1
direction: direction/reverse
top level: BBa_K1159203
start: 1
end: 67
orientation: inline

BBa_K1159306
type: ComponentDefinition
persistent identity: BBa K1159306
display id: BBa_K1159306
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Actin5 Promoter from Physcomitrella patens
creator: Ingmar Polte
created: 2013-09-13T11:00:00Z
modified: 2015-06-11T01:45:21Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1471_
m user id: 4206
owner id: 11507
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part contains the upstream region of the Actin5 gene from ''Physcomitrella patens'' which is one of the strongest promoter known in ''Physcomitrella patens''. This part is flanked by RFC[10]-non-coding pre- and suffix.
mutable notes: x
mutable provenance: ''Physcomitrella patens''
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: pAct5 from ''Physcomitrella patens''
sequence: https://synbiohub.org/public/igem/BBa_K1159306_sequence/1

pAct5 from ''Physcomitrella patens''
type: SequenceAnnotation
persistent identity: annotation2341585
display id: annotation2341585
version: 1
top level: BBa_K1159306
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1159306/annotation2341585/range2341585/1

https://synbiohub.org/public/igem/BBa_K1159306/annotation2341585/range2341585/1
type: Range
persistent identity: range2341585
display id: range2341585
version: 1
direction: direction/forward
top level: BBa_K1159306
start: 1
end: 1948
orientation: inline

BBa_K1825006
type: ComponentDefinition
persistent identity: BBa K1825006
display id: BBa_K1825006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: 35s CaMV + nptII
creator: Jonathan Asmund Arnesen
created: 2015-09-02T11:00:00Z
modified: 2015-12-16T12:18:33Z
discontinued: false
dominant: false
group u list: _2251_
m user id: 4206
owner id: 28047
owning group id: 9
status: status/Planning
bookmark: false
mutable description: The neomycin phosphotransferase gene driven by the 35s cauliflower mosaic virus promoter.
mutable notes: none
mutable provenance: Same as basic parts
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1825004
component: BBa_K1825005
sequence annotation: BBa_K1825004
sequence annotation: BBa_K1825005
sequence: https://synbiohub.org/public/igem/BBa_K1825006_sequence/1

BBa_K1825004
type: Component
persistent identity: component2443078
display id: component2443078
version: 1
top level: BBa_K1825006
access: public
definition: BBa_K1825004

BBa_K1825005
type: Component
persistent identity: component2443079
display id: component2443079
version: 1
top level: BBa_K1825006
access: public
definition: BBa_K1825005

BBa_K1825004
type: SequenceAnnotation
persistent identity: annotation2443078
display id: annotation2443078
version: 1
top level: BBa_K1825006
location: https://synbiohub.org/public/igem/BBa_K1825006/annotation2443078/range2443078/1
component: BBa_K1825004

https://synbiohub.org/public/igem/BBa_K1825006/annotation2443078/range2443078/1
type: Range
persistent identity: range2443078
display id: range2443078
version: 1
direction: direction/reverse
top level: BBa_K1825006
start: 1
end: 390
orientation: inline

BBa_K1825005
type: SequenceAnnotation
persistent identity: annotation2443079
display id: annotation2443079
version: 1
top level: BBa_K1825006
location: https://synbiohub.org/public/igem/BBa_K1825006/annotation2443079/range2443079/1
component: BBa_K1825005

https://synbiohub.org/public/igem/BBa_K1825006/annotation2443079/range2443079/1
type: Range
persistent identity: range2443079
display id: range2443079
version: 1
direction: direction/reverse
top level: BBa_K1825006
start: 397
end: 1191
orientation: inline

BBa_K1159120
type: ComponentDefinition
persistent identity: BBa K1159120
display id: BBa_K1159120
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Red light triggered TEV Protease with FRET Reporter for plants translation unit (PhyB/PIF6 version)
creator: Dong-Jiunn Jeffery TRUONG
created: 2013-09-15T11:00:00Z
modified: 2015-06-11T02:00:45Z
discontinued: false
dominant: false
group u list: _1471_
m user id: 4206
owner id: 11507
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: Red light triggered TEV Protease system with FRET Reporter for plants translation unit (PhyB/PIF6 version)
mutable notes: x
mutable provenance: Synthetic
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_K1159116
component: BBa_K1159112
component: BBa_K1159994
sequence annotation: BBa_K1159112
sequence annotation: BBa_K1159994
sequence annotation: BBa_K1159116
sequence: https://synbiohub.org/public/igem/BBa_K1159120_sequence/1

BBa_K1159116
type: Component
persistent identity: component2348460
display id: component2348460
version: 1
top level: BBa_K1159120
access: public
definition: https://synbiohub.org/public/igem/BBa_K1159116/1

BBa_K1159112
type: Component
persistent identity: component2348473
display id: component2348473
version: 1
top level: BBa_K1159120
access: public
definition: https://synbiohub.org/public/igem/BBa_K1159112/1

BBa_K1159994
type: Component
persistent identity: component2348462
display id: component2348462
version: 1
top level: BBa_K1159120
access: public
definition: https://synbiohub.org/public/igem/BBa_K1159994/1

BBa_K1159112
type: SequenceAnnotation
persistent identity: annotation2348473
display id: annotation2348473
version: 1
top level: BBa_K1159120
location: https://synbiohub.org/public/igem/BBa_K1159120/annotation2348473/range2348473/1
component: BBa_K1159112

https://synbiohub.org/public/igem/BBa_K1159120/annotation2348473/range2348473/1
type: Range
persistent identity: range2348473
display id: range2348473
version: 1
direction: direction/reverse
top level: BBa_K1159120
start: 5285
end: 6751
orientation: inline

BBa_K1159994
type: SequenceAnnotation
persistent identity: annotation2348462
display id: annotation2348462
version: 1
top level: BBa_K1159120
location: https://synbiohub.org/public/igem/BBa_K1159120/annotation2348462/range2348462/1
component: BBa_K1159994

https://synbiohub.org/public/igem/BBa_K1159120/annotation2348462/range2348462/1
type: Range
persistent identity: range2348462
display id: range2348462
version: 1
direction: direction/reverse
top level: BBa_K1159120
start: 5270
end: 5284
orientation: inline

BBa_K1159116
type: SequenceAnnotation
persistent identity: annotation2348460
display id: annotation2348460
version: 1
top level: BBa_K1159120
location: https://synbiohub.org/public/igem/BBa_K1159120/annotation2348460/range2348460/1
component: BBa_K1159116

https://synbiohub.org/public/igem/BBa_K1159120/annotation2348460/range2348460/1
type: Range
persistent identity: range2348460
display id: range2348460
version: 1
direction: direction/reverse
top level: BBa_K1159120
start: 1
end: 5269
orientation: inline

BBa_K1159205
type: ComponentDefinition
persistent identity: BBa K1159205
display id: BBa_K1159205
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Secretory SpyTag (SERK-SigP_SpyTag) in RFC[25] N-Part
creator: Dong-Jiunn Jeffery TRUONG
created: 2013-09-16T11:00:00Z
modified: 2015-06-11T01:59:37Z
discontinued: false
dominant: false
group u list: _1471_
m user id: 4206
owner id: 11507
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This part encodes the SpyTag [http://parts.igem.org/Part:BBa_K1159201 BBa_K1159201] with a N-terminal signal peptide for secretion (SERK receptor signal peptide from ''Physcomitrella patens''). This construct is flanked by RFC[25] N-part prefix and suffix. Note: This means only protein fusions to the C-terminus of this part is possible, adding promoters (typically RFC[10]) into the prefix or terminators/IRES (typically RFC[10]) into the suffix of this part is nevertheless possible.
mutable notes: x
mutable provenance: Synthetic
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_K1159303
component: BBa_K1159201
component: BBa_K1159993
sequence annotation: BBa_K1159993
sequence annotation: BBa_K1159201
sequence annotation: BBa_K1159303
sequence: https://synbiohub.org/public/igem/BBa_K1159205_sequence/1

BBa_K1159303
type: Component
persistent identity: component2348898
display id: component2348898
version: 1
top level: BBa_K1159205
access: public
definition: BBa_K1159303

BBa_K1159201
type: Component
persistent identity: component2348903
display id: component2348903
version: 1
top level: BBa_K1159205
access: public
definition: https://synbiohub.org/public/igem/BBa_K1159201/1

BBa_K1159993
type: Component
persistent identity: component2348900
display id: component2348900
version: 1
top level: BBa_K1159205
access: public
definition: https://synbiohub.org/public/igem/BBa_K1159993/1

BBa_K1159993
type: SequenceAnnotation
persistent identity: annotation2348900
display id: annotation2348900
version: 1
top level: BBa_K1159205
location: https://synbiohub.org/public/igem/BBa_K1159205/annotation2348900/range2348900/1
component: BBa_K1159993

https://synbiohub.org/public/igem/BBa_K1159205/annotation2348900/range2348900/1
type: Range
persistent identity: range2348900
display id: range2348900
version: 1
direction: direction/reverse
top level: BBa_K1159205
start: 101
end: 106
orientation: inline

BBa_K1159201
type: SequenceAnnotation
persistent identity: annotation2348903
display id: annotation2348903
version: 1
top level: BBa_K1159205
location: https://synbiohub.org/public/igem/BBa_K1159205/annotation2348903/range2348903/1
component: BBa_K1159201

https://synbiohub.org/public/igem/BBa_K1159205/annotation2348903/range2348903/1
type: Range
persistent identity: range2348903
display id: range2348903
version: 1
direction: direction/reverse
top level: BBa_K1159205
start: 107
end: 145
orientation: inline

BBa_K1159303
type: SequenceAnnotation
persistent identity: annotation2348898
display id: annotation2348898
version: 1
top level: BBa_K1159205
location: https://synbiohub.org/public/igem/BBa_K1159205/annotation2348898/range2348898/1
component: BBa_K1159303

https://synbiohub.org/public/igem/BBa_K1159205/annotation2348898/range2348898/1
type: Range
persistent identity: range2348898
display id: range2348898
version: 1
direction: direction/reverse
top level: BBa_K1159205
start: 1
end: 100
orientation: inline

BBa_K1159303
type: ComponentDefinition
persistent identity: BBa K1159303
display id: BBa_K1159303
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Signal Peptide of SERK Receptor from Physcomitrella patens in RFC[25]
creator: TU Munich 2013
created: 2013-09-13T11:00:00Z
modified: 2015-06-11T01:54:49Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1471_
m user id: 4206
owner id: 11507
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This part codes for the signal peptide from the SERK receptor (Somatic Embryogenesis RECEPTOR KINAS) of ''Physocmitrella patens''. The signal peptide induces translocation of the protein into the Endoplasmatic Reticulum (ER) for secretion or membrane integration. Protein fused to the C-terminus of this part should be secreted or otherwise if the part fused region contains a transmembrane domain it will be integrated in cytoplasmatic membrane. This part starts with a consensus sequence for ''Physcomitrella patens'' which allows efficient translation start. Furthermore, this part is flanked by RFC[25] N-part pre- and suffix for fusions to the C-terminus of this part.
mutable notes: x
mutable provenance: ''Physcomitrella patens''
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: Consensus Sequence for ''P. patens''
sequence annotation: SERK Signal Peptide
sequence: https://synbiohub.org/public/igem/BBa_K1159303_sequence/1

Consensus Sequence for ''P. patens''
type: SequenceAnnotation
persistent identity: annotation2341512
display id: annotation2341512
version: 1
top level: BBa_K1159303
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1159303/annotation2341512/range2341512/1

https://synbiohub.org/public/igem/BBa_K1159303/annotation2341512/range2341512/1
type: Range
persistent identity: range2341512
display id: range2341512
version: 1
direction: direction/forward
top level: BBa_K1159303
start: 1
end: 4
orientation: inline

SERK Signal Peptide
type: SequenceAnnotation
persistent identity: annotation2341513
display id: annotation2341513
version: 1
top level: BBa_K1159303
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1159303/annotation2341513/range2341513/1

https://synbiohub.org/public/igem/BBa_K1159303/annotation2341513/range2341513/1
type: Range
persistent identity: range2341513
display id: range2341513
version: 1
direction: direction/forward
top level: BBa_K1159303
start: 5
end: 100
orientation: inline

BBa_K1159308
type: ComponentDefinition
persistent identity: BBa K1159308
display id: BBa_K1159308
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Neomycin phosphotransferase II (nptII) plant expression cassette for G418 resistance
creator: Dong-Jiunn Jeffery TRUONG
created: 2013-09-13T11:00:00Z
modified: 2015-05-28T02:53:27Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1471_
m user id: 4206
owner id: 11507
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This part contains the expression cassette for the Neomycin Phosphotransferase II (nptII) responsible for G418 resistance. NptII is driven by NOS promoter and terminator. This part is flanked by RFC[10] pre- and suffix.
mutable notes: x
mutable provenance: Synthetic
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
sequence annotation: Neomycin phosphotransferase II
sequence annotation: NOS Terminator
sequence annotation: NOS Promoter
sequence: https://synbiohub.org/public/igem/BBa_K1159308_sequence/1

Neomycin phosphotransferase II
type: SequenceAnnotation
persistent identity: annotation2341660
display id: annotation2341660
version: 1
top level: BBa_K1159308
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1159308/annotation2341660/range2341660/1

https://synbiohub.org/public/igem/BBa_K1159308/annotation2341660/range2341660/1
type: Range
persistent identity: range2341660
display id: range2341660
version: 1
direction: direction/forward
top level: BBa_K1159308
start: 374
end: 1168
orientation: inline

NOS Terminator
type: SequenceAnnotation
persistent identity: annotation2341662
display id: annotation2341662
version: 1
top level: BBa_K1159308
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1159308/annotation2341662/range2341662/1

https://synbiohub.org/public/igem/BBa_K1159308/annotation2341662/range2341662/1
type: Range
persistent identity: range2341662
display id: range2341662
version: 1
direction: direction/forward
top level: BBa_K1159308
start: 1244
end: 1499
orientation: inline

NOS Promoter
type: SequenceAnnotation
persistent identity: annotation2341659
display id: annotation2341659
version: 1
top level: BBa_K1159308
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1159308/annotation2341659/range2341659/1

https://synbiohub.org/public/igem/BBa_K1159308/annotation2341659/range2341659/1
type: Range
persistent identity: range2341659
display id: range2341659
version: 1
direction: direction/forward
top level: BBa_K1159308
start: 55
end: 361
orientation: inline

BBa_K1159316
type: ComponentDefinition
persistent identity: BBa K1159316
display id: BBa_K1159316
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: SERK-Receptor TMD with C-terminal GFP and N-terminal Strep-tag II TEV-site linker in RFC[25]
creator: TU Munich 2013
created: 2013-09-15T11:00:00Z
modified: 2015-06-11T01:53:10Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1471_
m user id: 4206
owner id: 11507
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This part codes for the Transmembrane Domain of SERK Receptor from ''Physcomitrella patens'' with a C-terminal (intracellular) GFPmut1 fusion and a N-terminal fusion to a linker containing ''Strep''-tag II and a TEV cleavage site; SERK TMD and GFPmut1 are seperated by a middle long linker. This part is flanked by RFC[25] pre- and suffix for further protein fusions. Note: A signal peptide is needed for membrane integration.
mutable notes: x
mutable provenance: Synthetic
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_K1159993
component: BBa_K1159315
component: BBa_K1159309
sequence annotation: BBa_K1159993
sequence annotation: BBa_K1159315
sequence annotation: BBa_K1159309
sequence: https://synbiohub.org/public/igem/BBa_K1159316_sequence/1

BBa_K1159993
type: Component
persistent identity: component2346352
display id: component2346352
version: 1
top level: BBa_K1159316
access: public
definition: https://synbiohub.org/public/igem/BBa_K1159993/1

BBa_K1159315
type: Component
persistent identity: component2346363
display id: component2346363
version: 1
top level: BBa_K1159316
access: public
definition: BBa_K1159315

BBa_K1159309
type: Component
persistent identity: component2346350
display id: component2346350
version: 1
top level: BBa_K1159316
access: public
definition: https://synbiohub.org/public/igem/BBa_K1159309/1

BBa_K1159993
type: SequenceAnnotation
persistent identity: annotation2346352
display id: annotation2346352
version: 1
top level: BBa_K1159316
location: https://synbiohub.org/public/igem/BBa_K1159316/annotation2346352/range2346352/1
component: BBa_K1159993

https://synbiohub.org/public/igem/BBa_K1159316/annotation2346352/range2346352/1
type: Range
persistent identity: range2346352
display id: range2346352
version: 1
direction: direction/reverse
top level: BBa_K1159316
start: 58
end: 63
orientation: inline

BBa_K1159315
type: SequenceAnnotation
persistent identity: annotation2346363
display id: annotation2346363
version: 1
top level: BBa_K1159316
location: https://synbiohub.org/public/igem/BBa_K1159316/annotation2346363/range2346363/1
component: BBa_K1159315

https://synbiohub.org/public/igem/BBa_K1159316/annotation2346363/range2346363/1
type: Range
persistent identity: range2346363
display id: range2346363
version: 1
direction: direction/reverse
top level: BBa_K1159316
start: 64
end: 996
orientation: inline

BBa_K1159309
type: SequenceAnnotation
persistent identity: annotation2346350
display id: annotation2346350
version: 1
top level: BBa_K1159316
location: https://synbiohub.org/public/igem/BBa_K1159316/annotation2346350/range2346350/1
component: BBa_K1159309

https://synbiohub.org/public/igem/BBa_K1159316/annotation2346350/range2346350/1
type: Range
persistent identity: range2346350
display id: range2346350
version: 1
direction: direction/reverse
top level: BBa_K1159316
start: 1
end: 57
orientation: inline