1. Brekasis, D. and M. S. Paget (2003). "A novel sensor of NADH/NAD+ redox poise in Streptomyces coelicolor A3(2)." EMBO J 22(18): 4856-4865. 2. Deshpande, A. P. and S. S. Patel (2012). "Mechanism of transcription initiation by the yeast mitochondrial RNA polymerase." Biochim Biophys Acta. 3. http://regulondb.ccg.unam.mx/
This is regulatory sequence which can be repressed by binding of REX on ROP site.
With different place of ROP and promoter, the effect of REX binding on ROP might differs.
http://partsregistry.org/wiki/index.php?title=Part:BBa_K809007 http://partsregistry.org/wiki/index.php?title=Part:BBa_K809004 http://partsregistry.org/wiki/index.php?title=Part:BBa_K809009 http://partsregistry.org/wiki/index.php?title=Part:BBa_K809001
This biobrick is used to test the efficient of COB terminator.
The biobrick should be usable and all the biobricks won't interfere with each other.
http://partsregistry.org/wiki/index.php?title=Part:BBa_K809403 http://partsregistry.org/wiki/index.php?title=Part:BBa_K809001 http://partsregistry.org/wiki/index.php?title=Part:BBa_K809005
This biobrick is to produce GFP with the control of REX binding on ROP site.
All parts should be correctly linked without frame shift and Restriction Enzyme cutting site.
Mitochondrial shuttle plasmid carried mtGFP
Mitochondrial expression vector, with initial site ori7, up elements of cox2 gene (including promoter and RBS), and mtGFP.
http://partsregistry.org/wiki/index.php?title=Part:BBa_K809201 http://partsregistry.org/wiki/index.php?title=Part:BBa_K809401
REX is tagged with signal peptide of ZIM17, thus can be targetted into mitochondrial matrix.
The part BBa_K809201 and BBa_K809401 should be correctly linked without frame shift
SU9 comes from yeast gemome. REX was modified to suitable for expression in yeast.
This biobrick is the coding sequence of our SU9+REX in yeast cytoplasm. It is used to construct our final biobricks which express REX in the cytoplasm and then transport REX into mitochondrial matrix.
The signal peptid must be able to work efficiently, and can transport REX into mitochondrial matrix.
RBS sequence of yeast mitochondrial gene OLI1
This is obtained from Saccharomyces cerevisiae S288C mito-chromosome region. http://www.yeastgenome.org/
This is the RBS region for OLI1 gene. It is selected from Yeast mito-chromosome, and we want to test it in our experiment.
The sequence between the last ATG codon and CDS on the 3'UTR DNA sequence is believed to contain RBS. With some modifications, we made those sequences BioBricks.
http://partsregistry.org/wiki/index.php?title=Part:BBa_K809007 http://partsregistry.org/wiki/index.php?title=Part:BBa_K809006 http://partsregistry.org/wiki/index.php?title=Part:BBa_K809009 http://partsregistry.org/wiki/index.php?title=Part:BBa_K809001
This biobrick is used to test the efficient of Q0255 terminator.
1. Brekasis, D. and M. S. Paget (2003). "A novel sensor of NADH/NAD+ redox poise in Streptomyces coelicolor A3(2)." EMBO J 22(18): 4856-4865. 2. Deshpande, A. P. and S. S. Patel (2012). "Mechanism of transcription initiation by the yeast mitochondrial RNA polymerase." Biochim Biophys Acta.
Promoter of Yeast mitochondrial gene COX1/Oli1/15s rRNA
Deshpande, A. P. and S. S. Patel (2012). "Mechanism of transcription initiation by the yeast mitochondrial RNA polymerase." Biochim Biophys Acta.
This is the promoter region for COX1/Oli1/15s rRNA gene. It is selected from Yeast mito-chromosome, and we want to test it in our experiment.
This sequence is synthesized based on the sequences retrived.
RBS sequence of yeast mitochondrial gene AI1
This is the RBS region for AI1 gene. It is selected from Yeast mito-chromosome, and we want to test it in our experiment.
Zim17, a novel zinc finger protein essential for protein import into mitochondria. J Biol Chem. 2004 Nov 26 ;279(48):50243-9. Epub 2004 Sep 21 . http://www.uniprot.org/uniprot/P42844
this part is the sequence of transit signal peptide of ZIM17. It can help protein target to the mitochondrial matrix.
it is synthesized based on the complete sequence of signal peptide of ZIM17
Up elements (including promoter and RBS) of cox2 gene from Saccharomyces cerevisiae mitochondrial DNA.
Designed by Qiwu XU, BioBrick restriction enzyme sites added.
http://partsregistry.org/wiki/index.php?title=Part:BBa_K809007 http://partsregistry.org/wiki/index.php?title=Part:BBa_K809005 http://partsregistry.org/wiki/index.php?title=Part:BBa_K809009 http://partsregistry.org/wiki/index.php?title=Part:BBa_K809001
This biobrick is used to test the efficient of COX3 terminator.
NCBI database of Saccharomyces cerevisiae's genome
ori 7 region of Saccharomyces cerevisiae mtDNA, works as replication initial site.
Terminator of Yeast mitochondrial gene COX3
This is the terminator region for COX3 gene. It is selected from Yeast mito-chromosome, and we want to test it in our experiment.
We modelled the RNA secondary structure of COX3 3'UTR region, and found that the secondary structure of this region is very similar to bacterial terminator sequence.
Terminator of Yeast mitochondrial gene COB
It comes from Saccharomyces cerevisiae S288C mito-chromosome region
This is the terminator region for COB gene. It is selected from Yeast mito-chromosome, and we want to test it in our experiment.
We modelled the RNA secondary structure of COB 3'UTR region, and found that the secondary structure of this region is very similar to bacterial terminator sequence.
http://partsregistry.org/wiki/index.php?title=Part:BBa_K809402 http://partsregistry.org/wiki/index.php?title=Part:BBa_K809001 http://partsregistry.org/wiki/index.php?title=Part:BBa_K809005
Conversion of the iGEM parts registry to SBOL2.1