//chassis/prokaryote/salmonella
type: Collection
persistent identity: chassis prokaryote salmonella
display id: chassis_prokaryote_salmonella
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
owned by: james
owned by: myers
member: BBa_J64024
member: BBa_J64011
member: BBa_J64030
member: BBa_K864150
member: BBa_J64018
member: BBa_J64022
member: BBa_J64001
member: BBa_J64750
member: BBa_K112706
member: BBa_J64031
member: BBa_K112149
member: BBa_J64020
member: BBa_J64026
member: BBa_J64013
member: BBa_J64028
member: BBa_J64998
member: BBa_J64015
member: BBa_K112707

BBa_J64028
type: ComponentDefinition
persistent identity: BBa J64028
display id: BBa_J64028
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: sopB RBS
creator: Dan Widmaier
created: 2008-01-16T12:00:00Z
modified: 2015-08-31T01:56:26Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _98_
m user id: 0
owner id: 2407
owning group id: 98
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This part is the natural RBS for the sopB gene in the Salmonella Type III Secretion System.
mutable notes: This part was not designed with consideration for biobrick standard assembly in mind. This RBS hasn't been explicitly tested for expression rate in a Fluorescence or B-Gal assay.
mutable provenance: Salmonella LT2 Genomic DNA
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: sopB RBS
sequence: https://synbiohub.org/public/igem/BBa_J64028_sequence/1

sopB RBS
type: SequenceAnnotation
persistent identity: annotation1959632
display id: annotation1959632
version: 1
top level: BBa_J64028
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_J64028/annotation1959632/range1959632/1

https://synbiohub.org/public/igem/BBa_J64028/annotation1959632/range1959632/1
type: Range
persistent identity: range1959632
display id: range1959632
version: 1
direction: direction/forward
top level: BBa_J64028
start: 1
end: 20
orientation: inline

BBa_J64013
type: ComponentDefinition
persistent identity: BBa J64013
display id: BBa_J64013
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: sipA RBS
creator: Dan Widmaier
created: 2008-01-16T12:00:00Z
modified: 2015-08-31T01:56:26Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _98_
m user id: 0
owner id: 2407
owning group id: 98
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is the natural RBS that drives the sipA effector protein in the Salmonella SPI-1 system
mutable notes: This part was not designed with compatibility to biobrick standard assembly in mind. This RBS has not been explicitly tested in an expression assasy such as B-Gal or GFP expression.
mutable provenance: Salmonella LT2 Genomic DNA
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: sicA RBS
sequence: https://synbiohub.org/public/igem/BBa_J64013_sequence/1

sicA RBS
type: SequenceAnnotation
persistent identity: annotation1959612
display id: annotation1959612
version: 1
top level: BBa_J64013
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_J64013/annotation1959612/range1959612/1

https://synbiohub.org/public/igem/BBa_J64013/annotation1959612/range1959612/1
type: Range
persistent identity: range1959612
display id: range1959612
version: 1
direction: direction/forward
top level: BBa_J64013
start: 1
end: 18
orientation: inline

BBa_J64011
type: ComponentDefinition
persistent identity: BBa J64011
display id: BBa_J64011
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: invB RBS
creator: Dan Widmaier
created: 2008-01-15T12:00:00Z
modified: 2015-08-31T01:56:26Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _98_
m user id: 0
owner id: 2407
owning group id: 98
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: The natural RBS that drives invB
mutable notes: The expression level of this RBS has not been quantified.
mutable provenance: Salmonella LT2 Genomic DNA
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: invB RBS
sequence: https://synbiohub.org/public/igem/BBa_J64011_sequence/1

invB RBS
type: SequenceAnnotation
persistent identity: annotation1959604
display id: annotation1959604
version: 1
top level: BBa_J64011
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_J64011/annotation1959604/range1959604/1

https://synbiohub.org/public/igem/BBa_J64011/annotation1959604/range1959604/1
type: Range
persistent identity: range1959604
display id: range1959604
version: 1
direction: direction/forward
top level: BBa_J64011
start: 1
end: 19
orientation: inline

BBa_K112149
type: ComponentDefinition
persistent identity: BBa K112149
display id: BBa_K112149
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PmgtCB Magnesium promoter from Salmonella
creator: Madhvi Venkatesh
created: 2008-11-01T12:00:00Z
modified: 2015-05-08T01:09:14Z
discontinued: false
dominant: false
experience: experience/None
group u list: _224_
m user id: 0
owner id: 3023
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: PmgtCB magnesium promoter
mutable notes: This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at: BBb Standard Description Page
mutable provenance: genome
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K112149_sequence/1

BBa_J64020
type: ComponentDefinition
persistent identity: BBa J64020
display id: BBa_J64020
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: invI RBS
creator: Dan Widmaier
created: 2008-01-16T12:00:00Z
modified: 2015-08-31T01:56:26Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _98_
m user id: 0
owner id: 2407
owning group id: 98
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This part is the natural invI RBS from Salmonella Typhimurium LT2.
mutable notes: This part was not designed with consideration for biobrick standard assembly in mind. This RBS has not been explicitly tested for expression level in a fluorescence or B-Gal assay.
mutable provenance: Salmonella Typhimurium LT2 Genomic DNA
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: invI RBS
sequence: https://synbiohub.org/public/igem/BBa_J64020_sequence/1

invI RBS
type: SequenceAnnotation
persistent identity: annotation1959619
display id: annotation1959619
version: 1
top level: BBa_J64020
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_J64020/annotation1959619/range1959619/1

https://synbiohub.org/public/igem/BBa_J64020/annotation1959619/range1959619/1
type: Range
persistent identity: range1959619
display id: range1959619
version: 1
direction: direction/forward
top level: BBa_J64020
start: 1
end: 20
orientation: inline

BBa_J64998
type: ComponentDefinition
persistent identity: BBa J64998
display id: BBa_J64998
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: consensus -10 and rest from SP6
creator: Sai Duriseti
created: 2007-03-25T11:00:00Z
modified: 2015-05-08T01:08:18Z
discontinued: false
dominant: false
experience: experience/None
group u list: _98_
m user id: 0
owner id: 1428
owning group id: 98
sample status: Not in stock
status: status/Unavailable
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: Sigma 70 -10
sequence: https://synbiohub.org/public/igem/BBa_J64998_sequence/1

Sigma 70 -10
type: SequenceAnnotation
persistent identity: annotation1921745
display id: annotation1921745
version: 1
top level: BBa_J64998
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_J64998/annotation1921745/range1921745/1

https://synbiohub.org/public/igem/BBa_J64998/annotation1921745/range1921745/1
type: Range
persistent identity: range1921745
display id: range1921745
version: 1
direction: direction/forward
top level: BBa_J64998
start: 1
end: 19
orientation: inline

BBa_J64015
type: ComponentDefinition
persistent identity: BBa J64015
display id: BBa_J64015
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: sopA RBS
creator: Dan Widmaier
created: 2008-01-16T12:00:00Z
modified: 2015-08-31T01:56:26Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _98_
m user id: 0
owner id: 2407
owning group id: 98
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is the natural RBS associated with the sopA effector protein in the Salmonella SPI-1 system.
mutable notes: This part was not designed with compliance to biobrick standard assembly in mind. This RBS hasn't been explicitly tested in an expression assay such as GFP fluorescence or B-Gal expression.
mutable provenance: Salmonella LT2 Genomic DNA
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: sopA RBS
sequence: https://synbiohub.org/public/igem/BBa_J64015_sequence/1

sopA RBS
type: SequenceAnnotation
persistent identity: annotation1959614
display id: annotation1959614
version: 1
top level: BBa_J64015
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_J64015/annotation1959614/range1959614/1

https://synbiohub.org/public/igem/BBa_J64015/annotation1959614/range1959614/1
type: Range
persistent identity: range1959614
display id: range1959614
version: 1
direction: direction/forward
top level: BBa_J64015
start: 1
end: 16
orientation: inline

BBa_J64750
type: ComponentDefinition
persistent identity: BBa J64750
display id: BBa_J64750
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: SPI-1 TTSS secretion-linked promoter from Salmonella
creator: Jeffrey J Tabor
created: 2007-05-14T11:00:00Z
modified: 2015-05-08T01:08:17Z
discontinued: false
dominant: false
experience: experience/None
group u list: _98_
m user id: 0
owner id: 88
owning group id: 98
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This promoter is activated when functional SPI-1 type III secretion systems are present and functional in Salmonella typhimurium 1344.
mutable notes: The promoter includes the binding site for the transcription factor InvF. This transcription factor is activated by the SicA chaparone when the secretion system is active.
mutable provenance: This part was cloned from Salmonella typhimurium 1344 genomic DNA.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: start
sequence annotation: wt rbs
sequence: https://synbiohub.org/public/igem/BBa_J64750_sequence/1

start
type: SequenceAnnotation
persistent identity: annotation1932769
display id: annotation1932769
version: 1
top level: BBa_J64750
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_J64750/annotation1932769/range1932769/1

https://synbiohub.org/public/igem/BBa_J64750/annotation1932769/range1932769/1
type: Range
persistent identity: range1932769
display id: range1932769
version: 1
direction: direction/forward
top level: BBa_J64750
start: 165
end: 167
orientation: inline

wt rbs
type: SequenceAnnotation
persistent identity: annotation1932768
display id: annotation1932768
version: 1
top level: BBa_J64750
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_J64750/annotation1932768/range1932768/1

https://synbiohub.org/public/igem/BBa_J64750/annotation1932768/range1932768/1
type: Range
persistent identity: range1932768
display id: range1932768
version: 1
direction: direction/forward
top level: BBa_J64750
start: 153
end: 158
orientation: inline

BBa_K112707
type: ComponentDefinition
persistent identity: BBa K112707
display id: BBa_K112707
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Pspv from Salmonella
creator: Bing Xia
created: 2008-10-20T11:00:00Z
modified: 2015-05-08T01:09:18Z
discontinued: false
dominant: false
experience: experience/None
group u list: _224_
m user id: 0
owner id: 2998
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: The spv promoter. ---- This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at: [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
mutable provenance: Construction of <Pspv> basic part K112707 PCR of dv017/dv016 on pBca1037 (499bp, EcoRI/BamHI/DpnI) Sub into pBca1256 (EcoRI/BamHI, 2472+499, L) Product is pBca1256-K112707 ----------------------------------------------- dv017 Forward biobricking of <Pspv> cgataGAATTCatgAGATCTaGATCCTGTGATGTTTGGCG dv016 Reverse biobricking of <Pspv> cgttaGGATCCGATAATGTtTGCAGGGGAATTATTTTG
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K112707_sequence/1

BBa_J64026
type: ComponentDefinition
persistent identity: BBa J64026
display id: BBa_J64026
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: sigE RBS
creator: Dan Widmaier
created: 2008-01-16T12:00:00Z
modified: 2015-08-31T01:56:26Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _98_
m user id: 0
owner id: 2407
owning group id: 98
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is the natural RBS for the sigE chaperone (BBa_J64025) in Salmonella Typhimurium LT2.
mutable notes: This part was not designed with compatibility to biobrick standard assembly in mind. This RBS has not been explicitly assayed for expression rate.
mutable provenance: Salmonella LT2 Genomic DNA
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: sigE RBS
sequence: https://synbiohub.org/public/igem/BBa_J64026_sequence/1

sigE RBS
type: SequenceAnnotation
persistent identity: annotation1959625
display id: annotation1959625
version: 1
top level: BBa_J64026
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_J64026/annotation1959625/range1959625/1

https://synbiohub.org/public/igem/BBa_J64026/annotation1959625/range1959625/1
type: Range
persistent identity: range1959625
display id: range1959625
version: 1
direction: direction/forward
top level: BBa_J64026
start: 1
end: 18
orientation: inline

BBa_J64018
type: ComponentDefinition
persistent identity: BBa J64018
display id: BBa_J64018
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: invJ RBS
creator: Dan Widmaier
created: 2008-01-16T12:00:00Z
modified: 2015-08-31T01:56:26Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _98_
m user id: 0
owner id: 2407
owning group id: 98
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This part is the natural RBS associated with the invJ protein in Salmonella Typhimurium LT2.
mutable notes: This part was not designed with compliance to biobrick standard assembly in mind.
mutable provenance: Salmonella Typhimurium LT2 Genomic DNA
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: invJ RBS
sequence: https://synbiohub.org/public/igem/BBa_J64018_sequence/1

invJ RBS
type: SequenceAnnotation
persistent identity: annotation1959617
display id: annotation1959617
version: 1
top level: BBa_J64018
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_J64018/annotation1959617/range1959617/1

https://synbiohub.org/public/igem/BBa_J64018/annotation1959617/range1959617/1
type: Range
persistent identity: range1959617
display id: range1959617
version: 1
direction: direction/forward
top level: BBa_J64018
start: 1
end: 17
orientation: inline

BBa_J64001
type: ComponentDefinition
persistent identity: BBa J64001
display id: BBa_J64001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: psicA from Salmonella
creator: Dan Widmaier
created: 2008-01-14T12:00:00Z
modified: 2015-08-31T01:56:26Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _98_
m user id: 0
owner id: 2407
owning group id: 98
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: The sicA promoter is a regulatory element in the Salmonella SPI-1 secretion system. It controls the expression of secreted effector proteins in the natural system and turns on late. Please reference Temme et al, 2008, JMB for exact dynamics of this promoter and associated feedback mechanisms. This promoter only functions in Salmonella strains containing SPI-1.
mutable notes: This part can only be used in salmonella strains.
mutable provenance: Salmonella LT2 Genomic DNA
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -35 Hexamer
sequence annotation: -10 Hexamer
sequence annotation: InvF:SicA Binding Box
sequence annotation: sicA Promoter
sequence annotation: +1 Transcriptional Start Site
sequence: https://synbiohub.org/public/igem/BBa_J64001_sequence/1

-35 Hexamer
type: SequenceAnnotation
persistent identity: annotation1959629
display id: annotation1959629
version: 1
top level: BBa_J64001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J64001/annotation1959629/range1959629/1

https://synbiohub.org/public/igem/BBa_J64001/annotation1959629/range1959629/1
type: Range
persistent identity: range1959629
display id: range1959629
version: 1
direction: direction/forward
top level: BBa_J64001
start: 71
end: 77
orientation: inline

-10 Hexamer
type: SequenceAnnotation
persistent identity: annotation1959630
display id: annotation1959630
version: 1
top level: BBa_J64001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J64001/annotation1959630/range1959630/1

https://synbiohub.org/public/igem/BBa_J64001/annotation1959630/range1959630/1
type: Range
persistent identity: range1959630
display id: range1959630
version: 1
direction: direction/forward
top level: BBa_J64001
start: 94
end: 100
orientation: inline

InvF:SicA Binding Box
type: SequenceAnnotation
persistent identity: annotation1959628
display id: annotation1959628
version: 1
top level: BBa_J64001
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_J64001/annotation1959628/range1959628/1

https://synbiohub.org/public/igem/BBa_J64001/annotation1959628/range1959628/1
type: Range
persistent identity: range1959628
display id: range1959628
version: 1
direction: direction/forward
top level: BBa_J64001
start: 54
end: 65
orientation: inline

sicA Promoter
type: SequenceAnnotation
persistent identity: annotation1959627
display id: annotation1959627
version: 1
top level: BBa_J64001
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_J64001/annotation1959627/range1959627/1

https://synbiohub.org/public/igem/BBa_J64001/annotation1959627/range1959627/1
type: Range
persistent identity: range1959627
display id: range1959627
version: 1
direction: direction/forward
top level: BBa_J64001
start: 1
end: 143
orientation: inline

+1 Transcriptional Start Site
type: SequenceAnnotation
persistent identity: annotation1959631
display id: annotation1959631
version: 1
top level: BBa_J64001
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_J64001/annotation1959631/range1959631/1

https://synbiohub.org/public/igem/BBa_J64001/annotation1959631/range1959631/1
type: Range
persistent identity: range1959631
display id: range1959631
version: 1
direction: direction/forward
top level: BBa_J64001
start: 106
end: 106
orientation: inline

BBa_K864150
type: ComponentDefinition
persistent identity: BBa K864150
display id: BBa_K864150
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Cat-SacB cassette for scarless chromosomal knockout
creator: Erik Lundin
created: 2012-09-24T11:00:00Z
modified: 2015-05-08T01:13:37Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1124_
m user id: 0
owner id: 9827
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Cat-SacB Consists of chloramphenicol resistance marker BBa_P1004 and the Bacillus subtilis levansucrase SacB BBa_K322921
mutable notes: -
mutable provenance: Partsregistry
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K322921
component: BBa_P1004
sequence annotation: BBa_K322921
sequence annotation: BBa_P1004
sequence: https://synbiohub.org/public/igem/BBa_K864150_sequence/1

BBa_K322921
type: Component
persistent identity: component2197683
display id: component2197683
version: 1
top level: BBa_K864150
access: public
definition: https://synbiohub.org/public/igem/BBa_K322921/1

BBa_P1004
type: Component
persistent identity: component2197679
display id: component2197679
version: 1
top level: BBa_K864150
access: public
definition: https://synbiohub.org/public/igem/BBa_P1004/1

BBa_K322921
type: SequenceAnnotation
persistent identity: annotation2197683
display id: annotation2197683
version: 1
top level: BBa_K864150
location: https://synbiohub.org/public/igem/BBa_K864150/annotation2197683/range2197683/1
component: BBa_K322921

https://synbiohub.org/public/igem/BBa_K864150/annotation2197683/range2197683/1
type: Range
persistent identity: range2197683
display id: range2197683
version: 1
direction: direction/reverse
top level: BBa_K864150
start: 778
end: 2214
orientation: inline

BBa_P1004
type: SequenceAnnotation
persistent identity: annotation2197679
display id: annotation2197679
version: 1
top level: BBa_K864150
location: https://synbiohub.org/public/igem/BBa_K864150/annotation2197679/range2197679/1
component: BBa_P1004

https://synbiohub.org/public/igem/BBa_K864150/annotation2197679/range2197679/1
type: Range
persistent identity: range2197679
display id: range2197679
version: 1
direction: direction/reverse
top level: BBa_K864150
start: 1
end: 769
orientation: inline

BBa_J64022
type: ComponentDefinition
persistent identity: BBa J64022
display id: BBa_J64022
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: sicA RBS
creator: Dan Widmaier
created: 2008-01-16T12:00:00Z
modified: 2015-08-31T01:56:26Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _98_
m user id: 0
owner id: 2407
owning group id: 98
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is the natural RBS from the sicA chaperone (BBa_J6021) in the Salmonella Type III Secretion System.
mutable notes: This part was not designed with standard biobrick assembly in mind.
mutable provenance: Salmonella LT2 Genomic DNA
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: misc
sequence: https://synbiohub.org/public/igem/BBa_J64022_sequence/1

misc
type: SequenceAnnotation
persistent identity: annotation1959621
display id: annotation1959621
version: 1
top level: BBa_J64022
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J64022/annotation1959621/range1959621/1

https://synbiohub.org/public/igem/BBa_J64022/annotation1959621/range1959621/1
type: Range
persistent identity: range1959621
display id: range1959621
version: 1
direction: direction/forward
top level: BBa_J64022
start: 1
end: 20
orientation: inline

BBa_K112706
type: ComponentDefinition
persistent identity: BBa K112706
display id: BBa_K112706
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Pspv2 from Salmonella
creator: Bing Xia
created: 2008-10-20T11:00:00Z
modified: 2015-05-08T01:09:18Z
discontinued: false
dominant: false
experience: experience/None
group u list: _224_
m user id: 0
owner id: 2998
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: The spv2 promoter. ---- This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at: [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
mutable provenance: Construction of <Pspv2> basic part K112706 PCR of dv015/dv016 on pBca1037 (500bp, EcoRI/BamHI/DpnI) Sub into pBca1256 (EcoRI/BamHI, 2472+ 500, L) Product is pBca1256-K112706 ----------------------------------------------- dv015 Forward biobricking of <Pspv2> cgataGAATTCatgAGATCTccttatgcagcgtaagggccgcaac dv016 Reverse biobricking of <Pspv2> cgttaGGATCCGATAATGTtTGCAGGGGAATTATTTTG
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K112706_sequence/1

BBa_J64024
type: ComponentDefinition
persistent identity: BBa J64024
display id: BBa_J64024
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: sipC RBS
creator: Dan Widmaier
created: 2008-01-16T12:00:00Z
modified: 2015-08-31T01:56:26Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _98_
m user id: 0
owner id: 2407
owning group id: 98
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This part is the natural RBS from the sipC protein in Salmonella Type III Secretion.
mutable notes: This part was not designed with biobrick standard assembly in mind. This RBS has not been explicitly measured for expression rate using fluorescence or B-Gal assay.
mutable provenance: Salmonella LT2 Genomic DNA
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: sipC RBS
sequence: https://synbiohub.org/public/igem/BBa_J64024_sequence/1

sipC RBS
type: SequenceAnnotation
persistent identity: annotation1959623
display id: annotation1959623
version: 1
top level: BBa_J64024
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_J64024/annotation1959623/range1959623/1

https://synbiohub.org/public/igem/BBa_J64024/annotation1959623/range1959623/1
type: Range
persistent identity: range1959623
display id: range1959623
version: 1
direction: direction/forward
top level: BBa_J64024
start: 1
end: 15
orientation: inline

BBa_J64030
type: ComponentDefinition
persistent identity: BBa J64030
display id: BBa_J64030
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: sopD RBS
creator: Dan Widmaier
created: 2008-01-16T12:00:00Z
modified: 2015-08-31T01:56:26Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _98_
m user id: 0
owner id: 2407
owning group id: 98
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is the natural RBS from the sopD gene in Salmonella Type III Secretion.
mutable notes: This part was designed without consideration for biobrick standard assembly. This RBS has not been explicitly tested for expression rate.
mutable provenance: Salmonella LT2 Genomic DNA
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: sopD RBS
sequence: https://synbiohub.org/public/igem/BBa_J64030_sequence/1

sopD RBS
type: SequenceAnnotation
persistent identity: annotation1959634
display id: annotation1959634
version: 1
top level: BBa_J64030
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_J64030/annotation1959634/range1959634/1

https://synbiohub.org/public/igem/BBa_J64030/annotation1959634/range1959634/1
type: Range
persistent identity: range1959634
display id: range1959634
version: 1
direction: direction/forward
top level: BBa_J64030
start: 1
end: 17
orientation: inline

BBa_J64031
type: ComponentDefinition
persistent identity: BBa J64031
display id: BBa_J64031
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: sptP RBS
creator: Dan Widmaier
created: 2008-01-16T12:00:00Z
modified: 2015-08-31T01:56:26Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _98_
m user id: 0
owner id: 2407
owning group id: 98
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is the natural RBS for the sptP gene in Salmonella Type III Secretion
mutable notes: This part was designed without consideration for biobrick standard assembly. This RBS has not been explicitly tested for expression rate.
mutable provenance: Salmonella LT2 Genomic DNA
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: sptP RBS
sequence: https://synbiohub.org/public/igem/BBa_J64031_sequence/1

sptP RBS
type: SequenceAnnotation
persistent identity: annotation1959635
display id: annotation1959635
version: 1
top level: BBa_J64031
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_J64031/annotation1959635/range1959635/1

https://synbiohub.org/public/igem/BBa_J64031/annotation1959635/range1959635/1
type: Range
persistent identity: range1959635
display id: range1959635
version: 1
direction: direction/forward
top level: BBa_J64031
start: 1
end: 20
orientation: inline