This is the natural RBS from the sopD gene in Salmonella Type III Secretion.
This part was designed without consideration for biobrick standard assembly. This RBS has not been explicitly tested for expression rate.
The expression level of this RBS has not been quantified.
PmgtCB Magnesium promoter from Salmonella
This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at: BBb Standard Description Page
This is the natural RBS that drives the sipA effector protein in the Salmonella SPI-1 system
This part was not designed with compatibility to biobrick standard assembly in mind. This RBS has not been explicitly tested in an expression assasy such as B-Gal or GFP expression.
This is the natural RBS associated with the sopA effector protein in the Salmonella SPI-1 system.
This part was not designed with compliance to biobrick standard assembly in mind. This RBS hasn't been explicitly tested in an expression assay such as GFP fluorescence or B-Gal expression.
The spv promoter. ---- This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at: [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
Construction of <Pspv> basic part K112707 PCR of dv017/dv016 on pBca1037 (499bp, EcoRI/BamHI/DpnI) Sub into pBca1256 (EcoRI/BamHI, 2472+499, L) Product is pBca1256-K112707 ----------------------------------------------- dv017 Forward biobricking of <Pspv> cgataGAATTCatgAGATCTaGATCCTGTGATGTTTGGCG dv016 Reverse biobricking of <Pspv> cgttaGGATCCGATAATGTtTGCAGGGGAATTATTTTG
SPI-1 TTSS secretion-linked promoter from Salmonella
This promoter is activated when functional SPI-1 type III secretion systems are present and functional in Salmonella typhimurium 1344.
The promoter includes the binding site for the transcription factor InvF. This transcription factor is activated by the SicA chaparone when the secretion system is active.
This part was cloned from Salmonella typhimurium 1344 genomic DNA.
This is the natural RBS for the sptP gene in Salmonella Type III Secretion
The sicA promoter is a regulatory element in the Salmonella SPI-1 secretion system. It controls the expression of secreted effector proteins in the natural system and turns on late. Please reference Temme et al, 2008, JMB for exact dynamics of this promoter and associated feedback mechanisms. This promoter only functions in Salmonella strains containing SPI-1.
This part can only be used in salmonella strains.
This part is the natural RBS associated with the invJ protein in Salmonella Typhimurium LT2.
This part was not designed with compliance to biobrick standard assembly in mind.
The spv2 promoter. ---- This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at: [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
Construction of <Pspv2> basic part K112706 PCR of dv015/dv016 on pBca1037 (500bp, EcoRI/BamHI/DpnI) Sub into pBca1256 (EcoRI/BamHI, 2472+ 500, L) Product is pBca1256-K112706 ----------------------------------------------- dv015 Forward biobricking of <Pspv2> cgataGAATTCatgAGATCTccttatgcagcgtaagggccgcaac dv016 Reverse biobricking of <Pspv2> cgttaGGATCCGATAATGTtTGCAGGGGAATTATTTTG
This is the natural RBS from the sicA chaperone (BBa_J6021) in the Salmonella Type III Secretion System.
This part was not designed with standard biobrick assembly in mind.
This part is the natural invI RBS from Salmonella Typhimurium LT2.
This part was not designed with consideration for biobrick standard assembly in mind. This RBS has not been explicitly tested for expression level in a fluorescence or B-Gal assay.
This part is the natural RBS for the sopB gene in the Salmonella Type III Secretion System.
This part was not designed with consideration for biobrick standard assembly in mind. This RBS hasn't been explicitly tested for expression rate in a Fluorescence or B-Gal assay.
This is the natural RBS for the sigE chaperone (BBa_J64025) in Salmonella Typhimurium LT2.
This part was not designed with compatibility to biobrick standard assembly in mind. This RBS has not been explicitly assayed for expression rate.
Cat-SacB cassette for scarless chromosomal knockout
Cat-SacB Consists of chloramphenicol resistance marker BBa_P1004 and the Bacillus subtilis levansucrase SacB BBa_K322921
This part is the natural RBS from the sipC protein in Salmonella Type III Secretion.
This part was not designed with biobrick standard assembly in mind. This RBS has not been explicitly measured for expression rate using fluorescence or B-Gal assay.