//classic/composite/uncategorized
type: Collection
persistent identity: classic composite uncategorized
display id: classic_composite_uncategorized
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
owned by: james
owned by: myers
member: BBa_I0401
member: BBa_I7024
member: BBa_I14053
member: BBa_I13542
member: BBa_J06910
member: BBa_K864444
member: BBa_I14023
member: BBa_I12232
member: BBa_J06004
member: BBa_I13500
member: BBa_I5310
member: BBa_I14035
member: BBa_R8237
member: BBa_P0476
member: BBa_E0613
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member: BBa_J05302
member: BBa_I13280
member: BBa_I7000
member: BBa_J05315
member: BBa_I12011
member: BBa_I12054
member: BBa_I13304
member: BBa_J06917
member: BBa_J06904
member: BBa_E0601
member: BBa_I12053
member: HBsAg
member: BBa_I12233
member: BBa_J06905
member: BBa_I13017
member: BBa_I14029
member: BBa_I5220
member: BBa_E0612
member: BBa_I14059
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member: BBa_I0407
member: BBa_I0402
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member: BBa_I15004
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member: BBa_E5503
member: BBa_I14005
member: BBa_J05314
member: BBa_J05301
member: BBa_I0409
member: BBa_I13511
member: BBa_Q01511
member: BBa_I13507
member: BBa_I14024
member: BBa_I14054
member: BBa_I14011
member: BBa_I14041
member: BBa_I13710
member: BBa_J06906
member: BBa_I13279
member: BBa_J05304
member: BBa_E0611
member: BBa_I13538
member: BBa_I12052
member: BBa_I5410
member: BBa_I14037

BBa_J06900
type: ComponentDefinition
persistent identity: BBa J06900
display id: BBa_J06900
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LacI QPI regulated by lambda cI with mCherry
creator: ytwang
created: 2005-06-27T11:00:00Z
modified: 2015-08-31T04:08:19Z
discontinued: true
dominant: false
experience: experience/None
group u list: _20_
m user id: 0
owner id: 340
owning group id: 20
sample status: Discontinued
status: status/Deleted
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0034
component: BBa_C0012
component: BBa_B0010
component: BBa_B0012
component: BBa_R0051
component: BBa_E2060
component: BBa_R0011
sequence annotation: BBa_B0012
sequence annotation: BBa_C0012
sequence annotation: BBa_B0010
sequence annotation: BBa_B0034
sequence annotation: BBa_E2060
sequence annotation: BBa_R0051
sequence annotation: BBa_R0011
sequence: https://synbiohub.org/public/igem/BBa_J06900_sequence/1

BBa_B0034
type: Component
persistent identity: component1563535
display id: component1563535
version: 1
top level: BBa_J06900
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0034
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display id: component1563585
version: 1
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access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_C0012
type: Component
persistent identity: component1563545
display id: component1563545
version: 1
top level: BBa_J06900
access: public
definition: https://synbiohub.org/public/igem/BBa_C0012/1

BBa_B0010
type: Component
persistent identity: component1563604
display id: component1563604
version: 1
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access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0010
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version: 1
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access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0012
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display id: component1563562
version: 1
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BBa_B0012
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display id: component1563614
version: 1
top level: BBa_J06900
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_R0051
type: Component
persistent identity: component1563527
display id: component1563527
version: 1
top level: BBa_J06900
access: public
definition: https://synbiohub.org/public/igem/BBa_R0051/1

BBa_E2060
type: Component
persistent identity: component1563598
display id: component1563598
version: 1
top level: BBa_J06900
access: public
definition: https://synbiohub.org/public/igem/BBa_E2060/1

BBa_R0011
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persistent identity: component1563576
display id: component1563576
version: 1
top level: BBa_J06900
access: public
definition: https://synbiohub.org/public/igem/BBa_R0011/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1563614
display id: annotation1563614
version: 1
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BBa_C0012
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display id: annotation1563545
version: 1
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component: BBa_C0012

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type: Range
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display id: range1563545
version: 1
direction: direction/forward
top level: BBa_J06900
start: 76
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orientation: inline

BBa_B0010
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BBa_B0034
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display id: range1563585
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direction: direction/forward
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BBa_E2060
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BBa_B0012
type: SequenceAnnotation
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version: 1
top level: BBa_J06900
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component: BBa_B0012

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type: Range
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display id: range1563562
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BBa_R0051
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BBa_B0010
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type: Range
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direction: direction/forward
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BBa_R0011
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BBa_B0034
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display id: annotation1563535
version: 1
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component: BBa_B0034

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type: Range
persistent identity: range1563535
display id: range1563535
version: 1
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orientation: inline

BBa_I11061
type: ComponentDefinition
persistent identity: BBa I11061
display id: BBa_I11061
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: P22 half bit
creator: mschomp
created: 2004-09-02T11:00:00Z
modified: 2015-08-31T04:07:30Z
discontinued: false
dominant: false
experience: experience/Issues
group u list: _2_
m user id: 0
owner id: 102
owning group id: 7
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: half bit contains integrase and excisionase coding regions for p22 phage and attachment sites for lambda int/xis proteins that flip a unidirecitonal terminator
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: AAV
sequence annotation: spacer
sequence annotation: attP (lambda) (BBa_I11023)
sequence annotation: SacII
sequence annotation: stop
sequence annotation: SalI
sequence annotation: Xis (p22 Excisionase)
sequence annotation: LVA
sequence annotation: RBS (BBa_I11010)
sequence annotation: ApaI
sequence annotation: BBa_I11013
sequence annotation: attB (lambda) (BBa_I11022)
sequence annotation: Integrase p22
sequence annotation: BamHI
sequence annotation: MluI
sequence annotation: XbaI
sequence: https://synbiohub.org/public/igem/BBa_I11061_sequence/1

AAV
type: SequenceAnnotation
persistent identity: annotation1197661
display id: annotation1197661
version: 1
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role: SO:0000324
role: feature/tag
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type: Range
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display id: range1197661
version: 1
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start: 2014
end: 2046
orientation: inline

spacer
type: SequenceAnnotation
persistent identity: annotation1133711
display id: annotation1133711
version: 1
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role: SO:0000110
role: feature/misc
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spacer
type: SequenceAnnotation
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attP (lambda) (BBa_I11023)
type: SequenceAnnotation
persistent identity: annotation1131678
display id: annotation1131678
version: 1
top level: BBa_I11061
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I11061/annotation1131678/range1131678/1

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type: Range
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display id: range1131678
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orientation: inline

SacII
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display id: annotation1131232
version: 1
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role: SO:0000110
role: feature/misc
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type: Range
persistent identity: range1131232
display id: range1131232
version: 1
direction: direction/forward
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start: 1227
end: 1232
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1197466
display id: annotation1197466
version: 1
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role: SO:0000319
role: feature/stop
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type: Range
persistent identity: range1197466
display id: range1197466
version: 1
direction: direction/forward
top level: BBa_I11061
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end: 1223
orientation: inline

SalI
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persistent identity: annotation1130402
display id: annotation1130402
version: 1
top level: BBa_I11061
role: SO:0000110
role: feature/misc
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type: Range
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display id: range1130402
version: 1
direction: direction/forward
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orientation: inline

spacer
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role: SO:0000110
role: feature/misc
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type: Range
persistent identity: range1133476
display id: range1133476
version: 1
direction: direction/forward
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orientation: inline

Xis (p22 Excisionase)
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persistent identity: annotation1133646
display id: annotation1133646
version: 1
top level: BBa_I11061
role: feature/cds
role: SO:0000316
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type: Range
persistent identity: range1133646
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spacer
type: SequenceAnnotation
persistent identity: annotation1131908
display id: annotation1131908
version: 1
top level: BBa_I11061
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I11061/annotation1131908/range1131908/1

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type: Range
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display id: range1131908
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LVA
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display id: annotation1197445
version: 1
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role: SO:0000324
role: feature/tag
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spacer
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persistent identity: annotation1130910
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version: 1
top level: BBa_I11061
role: SO:0000110
role: feature/misc
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type: Range
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orientation: inline

RBS (BBa_I11010)
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display id: annotation1133577
version: 1
top level: BBa_I11061
role: feature/rbs
role: SO:0000139
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display id: range1133577
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direction: direction/forward
top level: BBa_I11061
start: 1655
end: 1660
orientation: inline

ApaI
type: SequenceAnnotation
persistent identity: annotation1133176
display id: annotation1133176
version: 1
top level: BBa_I11061
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I11061/annotation1133176/range1133176/1

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type: Range
persistent identity: range1133176
display id: range1133176
version: 1
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start: 1612
end: 1617
orientation: inline

BBa_I11013
type: SequenceAnnotation
persistent identity: annotation1131959
display id: annotation1131959
version: 1
top level: BBa_I11061
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_I11061/annotation1131959/range1131959/1

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type: Range
persistent identity: range1131959
display id: range1131959
version: 1
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start: 1480
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spacer
type: SequenceAnnotation
persistent identity: annotation1133598
display id: annotation1133598
version: 1
top level: BBa_I11061
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I11061/annotation1133598/range1133598/1

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type: Range
persistent identity: range1133598
display id: range1133598
version: 1
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attB (lambda) (BBa_I11022)
type: SequenceAnnotation
persistent identity: annotation1133350
display id: annotation1133350
version: 1
top level: BBa_I11061
role: feature/binding
role: SO:0001091
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type: Range
persistent identity: range1133350
display id: range1133350
version: 1
direction: direction/forward
top level: BBa_I11061
start: 1621
end: 1645
orientation: inline

Integrase p22
type: SequenceAnnotation
persistent identity: annotation1131152
display id: annotation1131152
version: 1
top level: BBa_I11061
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_I11061/annotation1131152/range1131152/1

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type: Range
persistent identity: range1131152
display id: range1131152
version: 1
direction: direction/forward
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start: 24
end: 1184
orientation: inline

BamHI
type: SequenceAnnotation
persistent identity: annotation1133527
display id: annotation1133527
version: 1
top level: BBa_I11061
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I11061/annotation1133527/range1133527/1

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type: Range
persistent identity: range1133527
display id: range1133527
version: 1
direction: direction/forward
top level: BBa_I11061
start: 1649
end: 1654
orientation: inline

spacer
type: SequenceAnnotation
persistent identity: annotation1133268
display id: annotation1133268
version: 1
top level: BBa_I11061
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I11061/annotation1133268/range1133268/1

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type: Range
persistent identity: range1133268
display id: range1133268
version: 1
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spacer
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RBS (BBa_I11010)
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BBa_I14050
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description: C12, C4 (weak RBS)
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created: 2004-08-03T11:00:00Z
modified: 2015-08-31T04:07:38Z
discontinued: false
dominant: false
experience: experience/None
group u list: _4_
m user id: 0
owner id: 171
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: p(Las) TetO, Weak RBS, RhlI + LVA
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owned by: myers
star: false
type: DnaRegion
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component: BBa_B0031
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sequence annotation: BBa_I14015
sequence annotation: BBa_B0031
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BBa_I14005
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was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: p(Rhl) . strong RBS . LacI
creator: Aditi Shrivastava, Jiwon Lee, Sarah Welch
created: 2004-07-25T11:00:00Z
modified: 2015-08-31T04:07:37Z
discontinued: false
dominant: false
experience: experience/None
group u list: _4_
m user id: 0
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sample status: Not in stock
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bookmark: false
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star: false
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description: 434 cI QPI regulated by Lambda cI with EYFP
creator: Yves Wang
created: 2005-07-31T11:00:00Z
modified: 2015-08-31T04:08:19Z
discontinued: false
dominant: false
experience: experience/None
group u list: _20_
m user id: 0
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sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: -- No description --
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owned by: myers
star: false
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BBa_B0034
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BBa_B0010
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BBa_E0030
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BBa_B0010
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BBa_I14020
type: ComponentDefinition
persistent identity: BBa I14020
display id: BBa_I14020
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: strong RBS . CinI
creator: ashrivas
created: 2004-08-01T11:00:00Z
modified: 2015-08-31T04:07:37Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _4_
m user id: 0
owner id: 166
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: BBa_B0030 BBa_C0076
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owned by: myers
star: false
type: DnaRegion
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sequence annotation: BBa_C0076
sequence annotation: BBa_B0030
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BBa_B0030
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version: 1
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BBa_C0076
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top level: BBa_I14020
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BBa_C0076
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BBa_I534160
type: ComponentDefinition
persistent identity: BBa I534160
display id: BBa_I534160
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: aiiA (LVA-) protein generator driven by pBad
creator: Barry Canton
created: 2004-08-11T11:00:00Z
modified: 2015-08-31T04:07:42Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_6_
m user id: 0
owner id: 135
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Useful for measuring the ability of aiiA to degrade HSL in a cell expressing LuxI
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0012
component: BBa_C0160
component: BBa_B0010
component: BBa_B0034
component: BBa_I0500
sequence annotation: BBa_I0500
sequence annotation: BBa_B0034
sequence annotation: BBa_B0012
sequence annotation: BBa_C0160
sequence annotation: BBa_B0010
sequence: https://synbiohub.org/public/igem/BBa_I534160_sequence/1

BBa_B0012
type: Component
persistent identity: component1043852
display id: component1043852
version: 1
top level: BBa_I534160
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_C0160
type: Component
persistent identity: component1043837
display id: component1043837
version: 1
top level: BBa_I534160
access: public
definition: https://synbiohub.org/public/igem/BBa_C0160/1

BBa_B0010
type: Component
persistent identity: component1043842
display id: component1043842
version: 1
top level: BBa_I534160
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0034
type: Component
persistent identity: component1043835
display id: component1043835
version: 1
top level: BBa_I534160
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_I0500
type: Component
persistent identity: component1043830
display id: component1043830
version: 1
top level: BBa_I534160
access: public
definition: https://synbiohub.org/public/igem/BBa_I0500/1

BBa_I0500
type: SequenceAnnotation
persistent identity: annotation1043830
display id: annotation1043830
version: 1
top level: BBa_I534160
location: https://synbiohub.org/public/igem/BBa_I534160/annotation1043830/range1043830/1
component: BBa_I0500

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type: Range
persistent identity: range1043830
display id: range1043830
version: 1
direction: direction/forward
top level: BBa_I534160
start: 1
end: 1210
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1043835
display id: annotation1043835
version: 1
top level: BBa_I534160
location: https://synbiohub.org/public/igem/BBa_I534160/annotation1043835/range1043835/1
component: BBa_B0034

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type: Range
persistent identity: range1043835
display id: range1043835
version: 1
direction: direction/forward
top level: BBa_I534160
start: 1219
end: 1230
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1043852
display id: annotation1043852
version: 1
top level: BBa_I534160
location: https://synbiohub.org/public/igem/BBa_I534160/annotation1043852/range1043852/1
component: BBa_B0012

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persistent identity: range1043852
display id: range1043852
version: 1
direction: direction/forward
top level: BBa_I534160
start: 2089
end: 2129
orientation: inline

BBa_C0160
type: SequenceAnnotation
persistent identity: annotation1043837
display id: annotation1043837
version: 1
top level: BBa_I534160
location: https://synbiohub.org/public/igem/BBa_I534160/annotation1043837/range1043837/1
component: BBa_C0160

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persistent identity: range1043837
display id: range1043837
version: 1
direction: direction/forward
top level: BBa_I534160
start: 1237
end: 1992
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1043842
display id: annotation1043842
version: 1
top level: BBa_I534160
location: https://synbiohub.org/public/igem/BBa_I534160/annotation1043842/range1043842/1
component: BBa_B0010

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type: Range
persistent identity: range1043842
display id: range1043842
version: 1
direction: direction/forward
top level: BBa_I534160
start: 2001
end: 2080
orientation: inline

BBa_Q03530
type: ComponentDefinition
persistent identity: BBa Q03530
display id: BBa_Q03530
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: QPI (B0033.C0053.B0015.R0053)
creator: Caitlin Conboy and Jennifer Braff
created: 2003-12-02T12:00:00Z
modified: 2015-05-08T01:14:13Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0012
component: BBa_R0053
component: BBa_B0010
component: BBa_B0033
component: BBa_C0053
sequence annotation: BBa_R0053
sequence annotation: BBa_B0010
sequence annotation: BBa_B0033
sequence annotation: BBa_C0053
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_Q03530_sequence/1

BBa_B0012
type: Component
persistent identity: component952611
display id: component952611
version: 1
top level: BBa_Q03530
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_R0053
type: Component
persistent identity: component952630
display id: component952630
version: 1
top level: BBa_Q03530
access: public
definition: https://synbiohub.org/public/igem/BBa_R0053/1

BBa_B0010
type: Component
persistent identity: component952601
display id: component952601
version: 1
top level: BBa_Q03530
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0033
type: Component
persistent identity: component952580
display id: component952580
version: 1
top level: BBa_Q03530
access: public
definition: https://synbiohub.org/public/igem/BBa_B0033/1

BBa_C0053
type: Component
persistent identity: component952593
display id: component952593
version: 1
top level: BBa_Q03530
access: public
definition: https://synbiohub.org/public/igem/BBa_C0053/1

BBa_R0053
type: SequenceAnnotation
persistent identity: annotation952630
display id: annotation952630
version: 1
top level: BBa_Q03530
location: https://synbiohub.org/public/igem/BBa_Q03530/annotation952630/range952630/1
component: BBa_R0053

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type: Range
persistent identity: range952630
display id: range952630
version: 1
direction: direction/forward
top level: BBa_Q03530
start: 875
end: 928
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation952601
display id: annotation952601
version: 1
top level: BBa_Q03530
location: https://synbiohub.org/public/igem/BBa_Q03530/annotation952601/range952601/1
component: BBa_B0010

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type: Range
persistent identity: range952601
display id: range952601
version: 1
direction: direction/forward
top level: BBa_Q03530
start: 738
end: 817
orientation: inline

BBa_B0033
type: SequenceAnnotation
persistent identity: annotation952580
display id: annotation952580
version: 1
top level: BBa_Q03530
location: https://synbiohub.org/public/igem/BBa_Q03530/annotation952580/range952580/1
component: BBa_B0033

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type: Range
persistent identity: range952580
display id: range952580
version: 1
direction: direction/forward
top level: BBa_Q03530
start: 1
end: 11
orientation: inline

BBa_C0053
type: SequenceAnnotation
persistent identity: annotation952593
display id: annotation952593
version: 1
top level: BBa_Q03530
location: https://synbiohub.org/public/igem/BBa_Q03530/annotation952593/range952593/1
component: BBa_C0053

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type: Range
persistent identity: range952593
display id: range952593
version: 1
direction: direction/forward
top level: BBa_Q03530
start: 18
end: 704
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation952611
display id: annotation952611
version: 1
top level: BBa_Q03530
location: https://synbiohub.org/public/igem/BBa_Q03530/annotation952611/range952611/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_Q03530/annotation952611/range952611/1
type: Range
persistent identity: range952611
display id: range952611
version: 1
direction: direction/forward
top level: BBa_Q03530
start: 826
end: 866
orientation: inline

HBsAg
type: ComponentDefinition
persistent identity: BBa K1908002
display id: BBa_K1908002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Blue Chromoprotein + 2a + HBsAg + Yeast Secretion Tag + ADH1 Terminator
creator: Brian Yang, Jonathan Krog, Sunil Deochand, Jonathan Kelly
created: 2016-10-13T11:00:00Z
modified: 2016-10-19T12:14:56Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2374_
m user id: 29538
owner id: 29544
owning group id: 9
status: status/Planning
bookmark: false
mutable description: .
mutable notes: .
mutable provenance: .
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: S. Cerevisiae Kozak Sequence
sequence annotation: ADH1 Terminator
sequence annotation: Yeast Secretion Tag
sequence annotation: HBsAg
sequence annotation: 2A Peptide
sequence annotation: amilCP - Blue Chromoprotein
sequence: https://synbiohub.org/public/igem/BBa_K1908002_sequence/1

S. Cerevisiae Kozak Sequence
type: SequenceAnnotation
persistent identity: annotation2521820
display id: annotation2521820
version: 1
top level: HBsAg
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1908002/annotation2521820/range2521820/1

https://synbiohub.org/public/igem/BBa_K1908002/annotation2521820/range2521820/1
type: Range
persistent identity: range2521820
display id: range2521820
version: 1
direction: direction/forward
top level: HBsAg
start: 1
end: 10
orientation: inline

ADH1 Terminator
type: SequenceAnnotation
persistent identity: annotation2522045
display id: annotation2522045
version: 1
top level: HBsAg
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1908002/annotation2522045/range2522045/1

https://synbiohub.org/public/igem/BBa_K1908002/annotation2522045/range2522045/1
type: Range
persistent identity: range2522045
display id: range2522045
version: 1
direction: direction/forward
top level: HBsAg
start: 1538
end: 1666
orientation: inline

Yeast Secretion Tag
type: SequenceAnnotation
persistent identity: annotation2521953
display id: annotation2521953
version: 1
top level: HBsAg
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1908002/annotation2521953/range2521953/1

https://synbiohub.org/public/igem/BBa_K1908002/annotation2521953/range2521953/1
type: Range
persistent identity: range2521953
display id: range2521953
version: 1
direction: direction/forward
top level: HBsAg
start: 1418
end: 1537
orientation: inline

HBsAg
type: SequenceAnnotation
persistent identity: annotation2521924
display id: annotation2521924
version: 1
top level: HBsAg
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1908002/annotation2521924/range2521924/1

https://synbiohub.org/public/igem/BBa_K1908002/annotation2521924/range2521924/1
type: Range
persistent identity: range2521924
display id: range2521924
version: 1
direction: direction/forward
top level: HBsAg
start: 740
end: 1417
orientation: inline

2A Peptide
type: SequenceAnnotation
persistent identity: annotation2521934
display id: annotation2521934
version: 1
top level: HBsAg
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1908002/annotation2521934/range2521934/1

https://synbiohub.org/public/igem/BBa_K1908002/annotation2521934/range2521934/1
type: Range
persistent identity: range2521934
display id: range2521934
version: 1
direction: direction/forward
top level: HBsAg
start: 683
end: 739
orientation: inline

amilCP - Blue Chromoprotein
type: SequenceAnnotation
persistent identity: annotation2521893
display id: annotation2521893
version: 1
top level: HBsAg
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1908002/annotation2521893/range2521893/1

https://synbiohub.org/public/igem/BBa_K1908002/annotation2521893/range2521893/1
type: Range
persistent identity: range2521893
display id: range2521893
version: 1
direction: direction/forward
top level: HBsAg
start: 11
end: 682
orientation: inline

BBa_E0610
type: ComponentDefinition
persistent identity: BBa E0610
display id: BBa_E0610
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: I0500.Q04121.E0430
creator: cconboy
created: 2004-05-13T11:00:00Z
modified: 2015-08-31T04:07:26Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_1_
m user id: 0
owner id: 60
owning group id: 7
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_E0430
component: BBa_Q04121
component: BBa_I0500
sequence annotation: BBa_E0430
sequence annotation: BBa_I0500
sequence annotation: BBa_Q04121
sequence: https://synbiohub.org/public/igem/BBa_E0610_sequence/1

BBa_E0430
type: Component
persistent identity: component2228594
display id: component2228594
version: 1
top level: BBa_E0610
access: public
definition: https://synbiohub.org/public/igem/BBa_E0430/1

BBa_Q04121
type: Component
persistent identity: component2228584
display id: component2228584
version: 1
top level: BBa_E0610
access: public
definition: https://synbiohub.org/public/igem/BBa_Q04121/1

BBa_I0500
type: Component
persistent identity: component2228565
display id: component2228565
version: 1
top level: BBa_E0610
access: public
definition: https://synbiohub.org/public/igem/BBa_I0500/1

BBa_E0430
type: SequenceAnnotation
persistent identity: annotation2228594
display id: annotation2228594
version: 1
top level: BBa_E0610
location: https://synbiohub.org/public/igem/BBa_E0610/annotation2228594/range2228594/1
component: BBa_E0430

https://synbiohub.org/public/igem/BBa_E0610/annotation2228594/range2228594/1
type: Range
persistent identity: range2228594
display id: range2228594
version: 1
direction: direction/reverse
top level: BBa_E0610
start: 2598
end: 3475
orientation: inline

BBa_I0500
type: SequenceAnnotation
persistent identity: annotation2228565
display id: annotation2228565
version: 1
top level: BBa_E0610
location: https://synbiohub.org/public/igem/BBa_E0610/annotation2228565/range2228565/1
component: BBa_I0500

https://synbiohub.org/public/igem/BBa_E0610/annotation2228565/range2228565/1
type: Range
persistent identity: range2228565
display id: range2228565
version: 1
direction: direction/reverse
top level: BBa_E0610
start: 1
end: 1210
orientation: inline

BBa_Q04121
type: SequenceAnnotation
persistent identity: annotation2228584
display id: annotation2228584
version: 1
top level: BBa_E0610
location: https://synbiohub.org/public/igem/BBa_E0610/annotation2228584/range2228584/1
component: BBa_Q04121

https://synbiohub.org/public/igem/BBa_E0610/annotation2228584/range2228584/1
type: Range
persistent identity: range2228584
display id: range2228584
version: 1
direction: direction/reverse
top level: BBa_E0610
start: 1219
end: 2589
orientation: inline

BBa_J110001
type: ComponentDefinition
persistent identity: BBa J110001
display id: BBa_J110001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pulse generator/feedback controller
creator: ingalls
created: 2005-06-20T11:00:00Z
modified: 2015-08-31T04:08:25Z
discontinued: false
dominant: false
experience: experience/None
group u list: _38_
m user id: 0
owner id: 337
owning group id: 38
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: input is luxI output is gfp negative feedback through cI Increases in level of luxI will cause transient pulses in cI, with levels returning to near zero asymptotically
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_R0065
component: BBa_I9200
component: BBa_B0032
component: BBa_B0025
component: BBa_E0040
sequence annotation: BBa_B0025
sequence annotation: BBa_B0032
sequence annotation: BBa_R0065
sequence annotation: BBa_I9200
sequence annotation: BBa_E0040
sequence: https://synbiohub.org/public/igem/BBa_J110001_sequence/1

BBa_R0065
type: Component
persistent identity: component2223620
display id: component2223620
version: 1
top level: BBa_J110001
access: public
definition: https://synbiohub.org/public/igem/BBa_R0065/1

BBa_I9200
type: Component
persistent identity: component2223633
display id: component2223633
version: 1
top level: BBa_J110001
access: public
definition: https://synbiohub.org/public/igem/BBa_I9200/1

BBa_B0032
type: Component
persistent identity: component2223629
display id: component2223629
version: 1
top level: BBa_J110001
access: public
definition: https://synbiohub.org/public/igem/BBa_B0032/1

BBa_B0025
type: Component
persistent identity: component2223641
display id: component2223641
version: 1
top level: BBa_J110001
access: public
definition: https://synbiohub.org/public/igem/BBa_B0025/1

BBa_E0040
type: Component
persistent identity: component2223638
display id: component2223638
version: 1
top level: BBa_J110001
access: public
definition: https://synbiohub.org/public/igem/BBa_E0040/1

BBa_B0032
type: Component
persistent identity: component2223635
display id: component2223635
version: 1
top level: BBa_J110001
access: public
definition: https://synbiohub.org/public/igem/BBa_B0032/1

BBa_B0025
type: SequenceAnnotation
persistent identity: annotation2223641
display id: annotation2223641
version: 1
top level: BBa_J110001
location: https://synbiohub.org/public/igem/BBa_J110001/annotation2223641/range2223641/1
component: BBa_B0025

https://synbiohub.org/public/igem/BBa_J110001/annotation2223641/range2223641/1
type: Range
persistent identity: range2223641
display id: range2223641
version: 1
direction: direction/reverse
top level: BBa_J110001
start: 1622
end: 1750
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation2223629
display id: annotation2223629
version: 1
top level: BBa_J110001
location: https://synbiohub.org/public/igem/BBa_J110001/annotation2223629/range2223629/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_J110001/annotation2223629/range2223629/1
type: Range
persistent identity: range2223629
display id: range2223629
version: 1
direction: direction/forward
top level: BBa_J110001
start: 106
end: 118
orientation: inline

BBa_R0065
type: SequenceAnnotation
persistent identity: annotation2223620
display id: annotation2223620
version: 1
top level: BBa_J110001
location: https://synbiohub.org/public/igem/BBa_J110001/annotation2223620/range2223620/1
component: BBa_R0065

https://synbiohub.org/public/igem/BBa_J110001/annotation2223620/range2223620/1
type: Range
persistent identity: range2223620
display id: range2223620
version: 1
direction: direction/forward
top level: BBa_J110001
start: 1
end: 97
orientation: inline

BBa_I9200
type: SequenceAnnotation
persistent identity: annotation2223633
display id: annotation2223633
version: 1
top level: BBa_J110001
location: https://synbiohub.org/public/igem/BBa_J110001/annotation2223633/range2223633/1
component: BBa_I9200

https://synbiohub.org/public/igem/BBa_J110001/annotation2223633/range2223633/1
type: Range
persistent identity: range2223633
display id: range2223633
version: 1
direction: direction/reverse
top level: BBa_J110001
start: 125
end: 866
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation2223638
display id: annotation2223638
version: 1
top level: BBa_J110001
location: https://synbiohub.org/public/igem/BBa_J110001/annotation2223638/range2223638/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_J110001/annotation2223638/range2223638/1
type: Range
persistent identity: range2223638
display id: range2223638
version: 1
direction: direction/reverse
top level: BBa_J110001
start: 894
end: 1613
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation2223635
display id: annotation2223635
version: 1
top level: BBa_J110001
location: https://synbiohub.org/public/igem/BBa_J110001/annotation2223635/range2223635/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_J110001/annotation2223635/range2223635/1
type: Range
persistent identity: range2223635
display id: range2223635
version: 1
direction: direction/forward
top level: BBa_J110001
start: 875
end: 887
orientation: inline

Amp Cas
type: ComponentDefinition
persistent identity: BBa I13800
display id: BBa_I13800
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Ampicillin Resistance Cassette
creator: robertb
created: 2004-07-18T11:00:00Z
modified: 2015-08-31T04:07:36Z
discontinued: false
dominant: false
experience: experience/None
group u list: _6_
m user id: 0
owner id: 159
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is the wild-type cassette for ampicillin resistance.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: Ampicillin Resistance
sequence: https://synbiohub.org/public/igem/BBa_I13800_sequence/1

Ampicillin Resistance
type: SequenceAnnotation
persistent identity: annotation902300
display id: annotation902300
version: 1
top level: Amp Cas
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I13800/annotation902300/range902300/1

https://synbiohub.org/public/igem/BBa_I13800/annotation902300/range902300/1
type: Range
persistent identity: range902300
display id: range902300
version: 1
direction: direction/forward
top level: Amp Cas
start: 1
end: 861
orientation: inline

BBa_E0601
type: ComponentDefinition
persistent identity: BBa E0601
display id: BBa_E0601
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: I0500.Q04400.E0432
creator: Caitlin Conboy and Jennifer Braff
created: 2004-01-20T12:00:00Z
modified: 2015-08-31T04:07:26Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: I0500.Q04400.E0432
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0012
component: BBa_R0040
component: BBa_B0010
component: BBa_I0500
component: BBa_B0034
component: BBa_E0032
component: BBa_C0040
sequence annotation: BBa_R0040
sequence annotation: BBa_B0012
sequence annotation: BBa_B0010
sequence annotation: BBa_I0500
sequence annotation: BBa_B0034
sequence annotation: BBa_C0040
sequence annotation: BBa_E0032
sequence: https://synbiohub.org/public/igem/BBa_E0601_sequence/1

BBa_B0012
type: Component
persistent identity: component964163
display id: component964163
version: 1
top level: BBa_E0601
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_R0040
type: Component
persistent identity: component964125
display id: component964125
version: 1
top level: BBa_E0601
access: public
definition: https://synbiohub.org/public/igem/BBa_R0040/1

BBa_B0010
type: Component
persistent identity: component964153
display id: component964153
version: 1
top level: BBa_E0601
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_I0500
type: Component
persistent identity: component964079
display id: component964079
version: 1
top level: BBa_E0601
access: public
definition: https://synbiohub.org/public/igem/BBa_I0500/1

BBa_B0034
type: Component
persistent identity: component964133
display id: component964133
version: 1
top level: BBa_E0601
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0034
type: Component
persistent identity: component964084
display id: component964084
version: 1
top level: BBa_E0601
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0010
type: Component
persistent identity: component964100
display id: component964100
version: 1
top level: BBa_E0601
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_E0032
type: Component
persistent identity: component964145
display id: component964145
version: 1
top level: BBa_E0601
access: public
definition: https://synbiohub.org/public/igem/BBa_E0032/1

BBa_B0012
type: Component
persistent identity: component964110
display id: component964110
version: 1
top level: BBa_E0601
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_C0040
type: Component
persistent identity: component964094
display id: component964094
version: 1
top level: BBa_E0601
access: public
definition: https://synbiohub.org/public/igem/BBa_C0040/1

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation964125
display id: annotation964125
version: 1
top level: BBa_E0601
location: https://synbiohub.org/public/igem/BBa_E0601/annotation964125/range964125/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_E0601/annotation964125/range964125/1
type: Range
persistent identity: range964125
display id: range964125
version: 1
direction: direction/forward
top level: BBa_E0601
start: 2067
end: 2120
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation964163
display id: annotation964163
version: 1
top level: BBa_E0601
location: https://synbiohub.org/public/igem/BBa_E0601/annotation964163/range964163/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_E0601/annotation964163/range964163/1
type: Range
persistent identity: range964163
display id: range964163
version: 1
direction: direction/forward
top level: BBa_E0601
start: 3005
end: 3045
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation964100
display id: annotation964100
version: 1
top level: BBa_E0601
location: https://synbiohub.org/public/igem/BBa_E0601/annotation964100/range964100/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_E0601/annotation964100/range964100/1
type: Range
persistent identity: range964100
display id: range964100
version: 1
direction: direction/forward
top level: BBa_E0601
start: 1930
end: 2009
orientation: inline

BBa_I0500
type: SequenceAnnotation
persistent identity: annotation964079
display id: annotation964079
version: 1
top level: BBa_E0601
location: https://synbiohub.org/public/igem/BBa_E0601/annotation964079/range964079/1
component: BBa_I0500

https://synbiohub.org/public/igem/BBa_E0601/annotation964079/range964079/1
type: Range
persistent identity: range964079
display id: range964079
version: 1
direction: direction/forward
top level: BBa_E0601
start: 1
end: 1210
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation964084
display id: annotation964084
version: 1
top level: BBa_E0601
location: https://synbiohub.org/public/igem/BBa_E0601/annotation964084/range964084/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_E0601/annotation964084/range964084/1
type: Range
persistent identity: range964084
display id: range964084
version: 1
direction: direction/forward
top level: BBa_E0601
start: 1219
end: 1230
orientation: inline

BBa_C0040
type: SequenceAnnotation
persistent identity: annotation964094
display id: annotation964094
version: 1
top level: BBa_E0601
location: https://synbiohub.org/public/igem/BBa_E0601/annotation964094/range964094/1
component: BBa_C0040

https://synbiohub.org/public/igem/BBa_E0601/annotation964094/range964094/1
type: Range
persistent identity: range964094
display id: range964094
version: 1
direction: direction/forward
top level: BBa_E0601
start: 1237
end: 1896
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation964153
display id: annotation964153
version: 1
top level: BBa_E0601
location: https://synbiohub.org/public/igem/BBa_E0601/annotation964153/range964153/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_E0601/annotation964153/range964153/1
type: Range
persistent identity: range964153
display id: range964153
version: 1
direction: direction/forward
top level: BBa_E0601
start: 2917
end: 2996
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation964133
display id: annotation964133
version: 1
top level: BBa_E0601
location: https://synbiohub.org/public/igem/BBa_E0601/annotation964133/range964133/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_E0601/annotation964133/range964133/1
type: Range
persistent identity: range964133
display id: range964133
version: 1
direction: direction/forward
top level: BBa_E0601
start: 2129
end: 2140
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation964110
display id: annotation964110
version: 1
top level: BBa_E0601
location: https://synbiohub.org/public/igem/BBa_E0601/annotation964110/range964110/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_E0601/annotation964110/range964110/1
type: Range
persistent identity: range964110
display id: range964110
version: 1
direction: direction/forward
top level: BBa_E0601
start: 2018
end: 2058
orientation: inline

BBa_E0032
type: SequenceAnnotation
persistent identity: annotation964145
display id: annotation964145
version: 1
top level: BBa_E0601
location: https://synbiohub.org/public/igem/BBa_E0601/annotation964145/range964145/1
component: BBa_E0032

https://synbiohub.org/public/igem/BBa_E0601/annotation964145/range964145/1
type: Range
persistent identity: range964145
display id: range964145
version: 1
direction: direction/forward
top level: BBa_E0601
start: 2147
end: 2908
orientation: inline

BBa_Q03500
type: ComponentDefinition
persistent identity: BBa Q03500
display id: BBa_Q03500
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: QPI (B0033.C0050.B0015.R0050)
creator: Caitlin Conboy and Jennifer Braff
created: 2003-12-02T12:00:00Z
modified: 2015-05-08T01:14:13Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_R0050
component: BBa_C0050
component: BBa_B0012
component: BBa_B0010
component: BBa_B0033
sequence annotation: BBa_C0050
sequence annotation: BBa_R0050
sequence annotation: BBa_B0033
sequence annotation: BBa_B0012
sequence annotation: BBa_B0010
sequence: https://synbiohub.org/public/igem/BBa_Q03500_sequence/1

BBa_R0050
type: Component
persistent identity: component952391
display id: component952391
version: 1
top level: BBa_Q03500
access: public
definition: https://synbiohub.org/public/igem/BBa_R0050/1

BBa_C0050
type: Component
persistent identity: component952357
display id: component952357
version: 1
top level: BBa_Q03500
access: public
definition: https://synbiohub.org/public/igem/BBa_C0050/1

BBa_B0012
type: Component
persistent identity: component952375
display id: component952375
version: 1
top level: BBa_Q03500
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0010
type: Component
persistent identity: component952365
display id: component952365
version: 1
top level: BBa_Q03500
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0033
type: Component
persistent identity: component952341
display id: component952341
version: 1
top level: BBa_Q03500
access: public
definition: https://synbiohub.org/public/igem/BBa_B0033/1

BBa_C0050
type: SequenceAnnotation
persistent identity: annotation952357
display id: annotation952357
version: 1
top level: BBa_Q03500
location: https://synbiohub.org/public/igem/BBa_Q03500/annotation952357/range952357/1
component: BBa_C0050

https://synbiohub.org/public/igem/BBa_Q03500/annotation952357/range952357/1
type: Range
persistent identity: range952357
display id: range952357
version: 1
direction: direction/forward
top level: BBa_Q03500
start: 18
end: 761
orientation: inline

BBa_R0050
type: SequenceAnnotation
persistent identity: annotation952391
display id: annotation952391
version: 1
top level: BBa_Q03500
location: https://synbiohub.org/public/igem/BBa_Q03500/annotation952391/range952391/1
component: BBa_R0050

https://synbiohub.org/public/igem/BBa_Q03500/annotation952391/range952391/1
type: Range
persistent identity: range952391
display id: range952391
version: 1
direction: direction/forward
top level: BBa_Q03500
start: 932
end: 986
orientation: inline

BBa_B0033
type: SequenceAnnotation
persistent identity: annotation952341
display id: annotation952341
version: 1
top level: BBa_Q03500
location: https://synbiohub.org/public/igem/BBa_Q03500/annotation952341/range952341/1
component: BBa_B0033

https://synbiohub.org/public/igem/BBa_Q03500/annotation952341/range952341/1
type: Range
persistent identity: range952341
display id: range952341
version: 1
direction: direction/forward
top level: BBa_Q03500
start: 1
end: 11
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation952375
display id: annotation952375
version: 1
top level: BBa_Q03500
location: https://synbiohub.org/public/igem/BBa_Q03500/annotation952375/range952375/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_Q03500/annotation952375/range952375/1
type: Range
persistent identity: range952375
display id: range952375
version: 1
direction: direction/forward
top level: BBa_Q03500
start: 883
end: 923
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation952365
display id: annotation952365
version: 1
top level: BBa_Q03500
location: https://synbiohub.org/public/igem/BBa_Q03500/annotation952365/range952365/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_Q03500/annotation952365/range952365/1
type: Range
persistent identity: range952365
display id: range952365
version: 1
direction: direction/forward
top level: BBa_Q03500
start: 795
end: 874
orientation: inline

BBa_E0600
type: ComponentDefinition
persistent identity: BBa E0600
display id: BBa_E0600
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: I0500.Q04121.E0432
creator: Caitlin Conboy and Jennifer Braff
created: 2004-01-20T12:00:00Z
modified: 2015-08-31T04:07:26Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: I0500.Q04121.E0432
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_I0500
component: BBa_E0432
component: BBa_Q04121
sequence annotation: BBa_E0432
sequence annotation: BBa_I0500
sequence annotation: BBa_Q04121
sequence: https://synbiohub.org/public/igem/BBa_E0600_sequence/1

BBa_I0500
type: Component
persistent identity: component2228528
display id: component2228528
version: 1
top level: BBa_E0600
access: public
definition: https://synbiohub.org/public/igem/BBa_I0500/1

BBa_E0432
type: Component
persistent identity: component2228561
display id: component2228561
version: 1
top level: BBa_E0600
access: public
definition: https://synbiohub.org/public/igem/BBa_E0432/1

BBa_Q04121
type: Component
persistent identity: component2228547
display id: component2228547
version: 1
top level: BBa_E0600
access: public
definition: https://synbiohub.org/public/igem/BBa_Q04121/1

BBa_E0432
type: SequenceAnnotation
persistent identity: annotation2228561
display id: annotation2228561
version: 1
top level: BBa_E0600
location: https://synbiohub.org/public/igem/BBa_E0600/annotation2228561/range2228561/1
component: BBa_E0432

https://synbiohub.org/public/igem/BBa_E0600/annotation2228561/range2228561/1
type: Range
persistent identity: range2228561
display id: range2228561
version: 1
direction: direction/reverse
top level: BBa_E0600
start: 2598
end: 3514
orientation: inline

BBa_I0500
type: SequenceAnnotation
persistent identity: annotation2228528
display id: annotation2228528
version: 1
top level: BBa_E0600
location: https://synbiohub.org/public/igem/BBa_E0600/annotation2228528/range2228528/1
component: BBa_I0500

https://synbiohub.org/public/igem/BBa_E0600/annotation2228528/range2228528/1
type: Range
persistent identity: range2228528
display id: range2228528
version: 1
direction: direction/reverse
top level: BBa_E0600
start: 1
end: 1210
orientation: inline

BBa_Q04121
type: SequenceAnnotation
persistent identity: annotation2228547
display id: annotation2228547
version: 1
top level: BBa_E0600
location: https://synbiohub.org/public/igem/BBa_E0600/annotation2228547/range2228547/1
component: BBa_Q04121

https://synbiohub.org/public/igem/BBa_E0600/annotation2228547/range2228547/1
type: Range
persistent identity: range2228547
display id: range2228547
version: 1
direction: direction/reverse
top level: BBa_E0600
start: 1219
end: 2589
orientation: inline

BBa_I4285
type: ComponentDefinition
persistent identity: BBa I4285
display id: BBa_I4285
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Trans-splicing Tetrahymena ribozyme to remove LVA tag
creator: Austin Che
created: 2004-10-01T11:00:00Z
modified: 2015-08-31T04:07:41Z
discontinued: false
dominant: false
experience: experience/None
group u list: _41_
m user id: 0
owner id: 22
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Designed ribozyme to remove LVA tag from constructs built by Reshma containing a LVA tag on the YFP gene. Replace the LVA tag with a stop codon to allow for higher expression.
mutable notes: The ribozyme was designed for trans-splicing based on the design principles in Kohler et al 1999. The target sequence is a EYFP gene with a LVA degradation tag. The target splice site is the U closest to the end of the EYFP. The IGS (P1 helix) of the ribozyme was redesigned to bind to this region and an extended antisense region to the LVA tag of around 50 bp was added. The spliced on 3' exon is simply a stop codon. The expected behavior is for the LVA tag to be replaced by a stop codon, allowing for a return to the normal degradation rate for the protein (i.e. long life) and thus higher expression. Contains the same lac controlled promoter R0011 and double terminator used in the target construct.
mutable provenance: Tetrahymena thermophila
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence: https://synbiohub.org/public/igem/BBa_I4285_sequence/1

BBa_K621001
type: ComponentDefinition
persistent identity: BBa K621001
display id: BBa_K621001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: OMP-R-->RBS-->eYFP
creator: David Pohlman, Blythe Ferriere, Aile Able, Erica McFarland
created: 2011-09-26T11:00:00Z
modified: 2015-05-08T01:12:55Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _793_
m user id: 0
owner id: 4838
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: Our part consists of an OMP-R binding region that regulates our reporter gene, eYFP. The OMP-R binding region is an omp-c operator, which has three OMP-R binding sites. Our part is regulated by the ENVZ-OMPR two-component regulatory system. ENVZ is a osmolarity sensor on the inner membrane and phosphorylates OMP-R in the presence of high osmolarity. When phosphorylated, OMP-R binds to the omp-c operator and recruits RNAP to transcribe the downstream reporter gene. Fluorescence intensity due to the production of eYFP is a measure of the system's activity. When all three binding sites are occupied by phosphorylated OMP-R proteins, RNAP cannot bind to the DNA and transcribe downstream. This part can be used as an osmolarity indicator. Our team used this part as a glucose sensor because the presence of glucose changes osmolarity. Different concentrations of glucose produce different, quantifiable fluorescence intensities.
mutable notes: Our team had to combine the genes in a specific order. First our team cut out and purified the eYPF gene. We added this gene to a plasmid containing RBS. Next we cut out the RBS+eYFP intermediate and ligated them into the plasmid containing OMP-R. The RBS and OMP-R parts were too small to run out and extract on a gel because they would run off of the end of the gel. Therefore, we had to start the process with eYPF.
mutable provenance: This BioBrick was created from parts found in the Registry. These parts were BBa_R0082, BBa_B0032, and BBa_E0030.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_E0030
component: BBa_B0032
component: BBa_R0082
sequence annotation: BBa_E0030
sequence annotation: BBa_B0032
sequence annotation: BBa_R0082
sequence: https://synbiohub.org/public/igem/BBa_K621001_sequence/1

BBa_E0030
type: Component
persistent identity: component2145049
display id: component2145049
version: 1
top level: BBa_K621001
access: public
definition: https://synbiohub.org/public/igem/BBa_E0030/1

BBa_B0032
type: Component
persistent identity: component2145047
display id: component2145047
version: 1
top level: BBa_K621001
access: public
definition: https://synbiohub.org/public/igem/BBa_B0032/1

BBa_R0082
type: Component
persistent identity: component2145045
display id: component2145045
version: 1
top level: BBa_K621001
access: public
definition: https://synbiohub.org/public/igem/BBa_R0082/1

BBa_E0030
type: SequenceAnnotation
persistent identity: annotation2145049
display id: annotation2145049
version: 1
top level: BBa_K621001
location: https://synbiohub.org/public/igem/BBa_K621001/annotation2145049/range2145049/1
component: BBa_E0030

https://synbiohub.org/public/igem/BBa_K621001/annotation2145049/range2145049/1
type: Range
persistent identity: range2145049
display id: range2145049
version: 1
direction: direction/reverse
top level: BBa_K621001
start: 136
end: 858
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation2145047
display id: annotation2145047
version: 1
top level: BBa_K621001
location: https://synbiohub.org/public/igem/BBa_K621001/annotation2145047/range2145047/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_K621001/annotation2145047/range2145047/1
type: Range
persistent identity: range2145047
display id: range2145047
version: 1
direction: direction/forward
top level: BBa_K621001
start: 117
end: 129
orientation: inline

BBa_R0082
type: SequenceAnnotation
persistent identity: annotation2145045
display id: annotation2145045
version: 1
top level: BBa_K621001
location: https://synbiohub.org/public/igem/BBa_K621001/annotation2145045/range2145045/1
component: BBa_R0082

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modified: 2015-08-31T04:07:34Z
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display id: BBa_I5220
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Olmecs: Repressilator version 3 (434 cI)
creator: Olmecs 2003 IAP Team
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modified: 2015-08-31T04:07:42Z
discontinued: false
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start: 733
end: 812
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BBa_J06007
type: ComponentDefinition
persistent identity: BBa J06007
display id: BBa_J06007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: YFP and LuxI(LVA+) on LuxBox
creator: conniec
created: 2005-06-12T11:00:00Z
modified: 2015-08-31T04:08:16Z
discontinued: false
dominant: false
experience: experience/None
group u list: _20_
m user id: 0
owner id: 344
owning group id: 20
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Same as J06006, but also expresses LVA+ variant of LuxI.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_R0065
component: BBa_B0010
component: BBa_E0032
component: BBa_B0034
component: BBa_B0012
component: BBa_C0061
sequence annotation: BBa_E0032
sequence annotation: BBa_B0012
sequence annotation: BBa_R0065
sequence annotation: BBa_C0061
sequence annotation: BBa_B0034
sequence annotation: BBa_B0010
sequence: https://synbiohub.org/public/igem/BBa_J06007_sequence/1

BBa_R0065
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display id: component1562881
version: 1
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access: public
definition: https://synbiohub.org/public/igem/BBa_R0065/1

BBa_B0010
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definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_E0032
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definition: https://synbiohub.org/public/igem/BBa_E0032/1

BBa_B0034
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version: 1
top level: BBa_J06007
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0012
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BBa_B0034
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BBa_C0061
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access: public
definition: https://synbiohub.org/public/igem/BBa_C0061/1

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start: 1
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orientation: inline

BBa_C0061
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version: 1
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BBa_B0034
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BBa_C0430
type: ComponentDefinition
persistent identity: BBa C0430
display id: BBa_C0430
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS(B0034) + bglF
creator: T. Senthil
created: 2005-10-28T11:00:00Z
modified: 2015-08-31T04:07:24Z
discontinued: false
dominant: false
group u list: _44_
m user id: 0
owner id: 439
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sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Strong RBS + bgl sensor permease
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owned by: myers
star: false
type: DnaRegion
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BBa_C0420
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definition: https://synbiohub.org/public/igem/BBa_C0420/1

BBa_B0034
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version: 1
top level: BBa_C0430
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

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display id: range1744835
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direction: direction/forward
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start: 19
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orientation: inline

BBa_I13311
type: ComponentDefinition
persistent identity: BBa I13311
display id: BBa_I13311
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: I13305.E0430 Lac-Regulated Las Amplifier with YFP Reporter
creator: Jay and Debra and Barry
created: 2004-08-08T11:00:00Z
modified: 2015-08-31T04:07:33Z
discontinued: false
dominant: false
experience: experience/None
group u list: _6_
m user id: 0
owner id: 101
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_I13305
component: BBa_E0430
sequence annotation: BBa_E0430
sequence annotation: BBa_I13305
sequence: https://synbiohub.org/public/igem/BBa_I13311_sequence/1

BBa_I13305
type: Component
persistent identity: component2229611
display id: component2229611
version: 1
top level: BBa_I13311
access: public
definition: BBa_I13305

BBa_E0430
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definition: https://synbiohub.org/public/igem/BBa_E0430/1

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direction: direction/reverse
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BBa_I13305
type: SequenceAnnotation
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display id: annotation2229611
version: 1
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location: https://synbiohub.org/public/igem/BBa_I13311/annotation2229611/range2229611/1
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direction: direction/reverse
top level: BBa_I13311
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BBa_J06204
type: ComponentDefinition
persistent identity: BBa J06204
display id: BBa_J06204
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: C4HSL receiver , CI repressor hybrid
creator: sash31
created: 2005-06-23T11:00:00Z
modified: 2015-08-31T04:08:17Z
discontinued: false
dominant: false
experience: experience/None
group u list: _20_
m user id: 0
owner id: 339
owning group id: 20
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Takes an input of C4HSL and outputs CI
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_C0051
component: BBa_B0012
component: BBa_B0034
component: BBa_B0010
component: BBa_C0071
component: BBa_I14018
component: BBa_B0030
component: BBa_I14017
sequence annotation: BBa_I14017
sequence annotation: BBa_C0051
sequence annotation: BBa_B0034
sequence annotation: BBa_B0012
sequence annotation: BBa_B0010
sequence annotation: BBa_B0030
sequence annotation: BBa_C0071
sequence annotation: BBa_I14018
sequence: https://synbiohub.org/public/igem/BBa_J06204_sequence/1

BBa_C0051
type: Component
persistent identity: component1537028
display id: component1537028
version: 1
top level: BBa_J06204
access: public
definition: https://synbiohub.org/public/igem/BBa_C0051/1

BBa_B0012
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display id: component1536995
version: 1
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access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0034
type: Component
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display id: component1536969
version: 1
top level: BBa_J06204
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0010
type: Component
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version: 1
top level: BBa_J06204
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definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_C0071
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BBa_B0012
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BBa_B0010
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definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_I14018
type: Component
persistent identity: component1536964
display id: component1536964
version: 1
top level: BBa_J06204
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definition: https://synbiohub.org/public/igem/BBa_I14018/1

BBa_B0030
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definition: https://synbiohub.org/public/igem/BBa_B0030/1

BBa_I14017
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definition: https://synbiohub.org/public/igem/BBa_I14017/1

BBa_I14017
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BBa_B0012
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end: 985
orientation: inline

BBa_B0010
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display id: annotation1536985
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BBa_B0010
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display id: range1537034
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type: SequenceAnnotation
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display id: range1536979
version: 1
direction: direction/forward
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end: 823
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BBa_I14018
type: SequenceAnnotation
persistent identity: annotation1536964
display id: annotation1536964
version: 1
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persistent identity: range1536964
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BBa_I14022
type: ComponentDefinition
persistent identity: BBa I14022
display id: BBa_I14022
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: plac . strong RBS . YFP
creator: Aditi Shrivastava, Jiwon Lee, Sarah Welch
created: 2004-08-01T11:00:00Z
modified: 2015-08-31T04:07:37Z
discontinued: false
dominant: false
experience: experience/None
group u list: _4_
m user id: 0
owner id: 166
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: BBa_R0010 BBa_I14009
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0030
component: BBa_R0010
component: BBa_E0032
sequence annotation: BBa_E0032
sequence annotation: BBa_R0010
sequence annotation: BBa_B0030
sequence: https://synbiohub.org/public/igem/BBa_I14022_sequence/1

BBa_B0030
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display id: component1018403
version: 1
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access: public
definition: https://synbiohub.org/public/igem/BBa_B0030/1

BBa_R0010
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display id: component1018390
version: 1
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access: public
definition: https://synbiohub.org/public/igem/BBa_R0010/1

BBa_E0032
type: Component
persistent identity: component1018416
display id: component1018416
version: 1
top level: BBa_I14022
access: public
definition: https://synbiohub.org/public/igem/BBa_E0032/1

BBa_E0032
type: SequenceAnnotation
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display id: annotation1018416
version: 1
top level: BBa_I14022
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component: BBa_E0032

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type: Range
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display id: range1018416
version: 1
direction: direction/forward
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end: 991
orientation: inline

BBa_R0010
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version: 1
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BBa_B0030
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persistent identity: range1018403
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component: BBa_B0012
component: BBa_B0011
sequence annotation: BBa_B0011
sequence annotation: BBa_B0012
sequence annotation: BBa_E0032
sequence annotation: BBa_B0034
sequence annotation: BBa_R0053
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display id: component948091
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BBa_R0053
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definition: https://synbiohub.org/public/igem/BBa_R0053/1

BBa_E0032
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display id: component948103
version: 1
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definition: https://synbiohub.org/public/igem/BBa_E0032/1

BBa_B0012
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BBa_B0011
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persistent identity: component948123
display id: component948123
version: 1
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access: public
definition: https://synbiohub.org/public/igem/BBa_B0011/1

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type: SequenceAnnotation
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display id: annotation948123
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BBa_B0012
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end: 891
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BBa_E0032
type: SequenceAnnotation
persistent identity: annotation948103
display id: annotation948103
version: 1
top level: BBa_I5130
location: https://synbiohub.org/public/igem/BBa_I5130/annotation948103/range948103/1
component: BBa_E0032

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persistent identity: range948103
display id: range948103
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BBa_B0034
type: SequenceAnnotation
persistent identity: annotation948091
display id: annotation948091
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component: BBa_B0034

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persistent identity: range948091
display id: range948091
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BBa_R0053
type: SequenceAnnotation
persistent identity: annotation948081
display id: annotation948081
version: 1
top level: BBa_I5130
location: https://synbiohub.org/public/igem/BBa_I5130/annotation948081/range948081/1
component: BBa_R0053

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persistent identity: range948081
display id: range948081
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BBa_I13517
type: ComponentDefinition
persistent identity: BBa I13517
display id: BBa_I13517
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Screening plasmid intermediate
creator: jkm
created: 2005-06-05T11:00:00Z
modified: 2015-08-31T04:07:34Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_
m user id: 0
owner id: 253
owning group id: 6
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Built by Josh as an intermediate in screening plasmid construction
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0032
component: BBa_E1010
component: BBa_I0500
sequence annotation: BBa_B0032
sequence annotation: BBa_I0500
sequence annotation: BBa_E1010
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BBa_B0032
type: Component
persistent identity: component1505513
display id: component1505513
version: 1
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access: public
definition: https://synbiohub.org/public/igem/BBa_B0032/1

BBa_E1010
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display id: component1505521
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access: public
definition: https://synbiohub.org/public/igem/BBa_E1010/1

BBa_I0500
type: Component
persistent identity: component1505505
display id: component1505505
version: 1
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access: public
definition: https://synbiohub.org/public/igem/BBa_I0500/1

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display id: annotation1505513
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BBa_E1010
type: SequenceAnnotation
persistent identity: annotation1505521
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BBa_J05316
type: ComponentDefinition
persistent identity: BBa J05316
display id: BBa_J05316
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: R3 state + Reporter
creator: Alexander Roth
created: 2005-09-19T11:00:00Z
modified: 2015-08-31T04:08:15Z
discontinued: false
dominant: false
group u list: _34_
m user id: 0
owner id: 412
owning group id: 34
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_J05217
component: BBa_B0034
component: BBa_J05110
component: BBa_E0840
sequence annotation: BBa_J05110
sequence annotation: BBa_B0034
sequence annotation: BBa_J05217
sequence annotation: BBa_E0840
sequence: https://synbiohub.org/public/igem/BBa_J05316_sequence/1

BBa_J05217
type: Component
persistent identity: component2224956
display id: component2224956
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access: public
definition: https://synbiohub.org/public/igem/BBa_J05217/1

BBa_B0034
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access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_J05110
type: Component
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display id: component2224966
version: 1
top level: BBa_J05316
access: public
definition: https://synbiohub.org/public/igem/BBa_J05110/1

BBa_E0840
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display id: component2224977
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definition: https://synbiohub.org/public/igem/BBa_E0840/1

BBa_J05110
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BBa_J05217
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BBa_E0840
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direction: direction/reverse
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BBa_E5504
type: ComponentDefinition
persistent identity: BBa E5504
display id: BBa_E5504
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: R0040.B0032.E0040
creator: jcbraff
created: 2005-05-16T11:00:00Z
modified: 2015-08-31T04:07:27Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 61
owning group id: 1
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: R0040.B0032.E0040 (R0040.E5501)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_E0040
component: BBa_B0032
component: BBa_R0040
sequence annotation: BBa_R0040
sequence annotation: BBa_B0032
sequence annotation: BBa_E0040
sequence: https://synbiohub.org/public/igem/BBa_E5504_sequence/1

BBa_E0040
type: Component
persistent identity: component1489174
display id: component1489174
version: 1
top level: BBa_E5504
access: public
definition: https://synbiohub.org/public/igem/BBa_E0040/1

BBa_B0032
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persistent identity: component1489171
display id: component1489171
version: 1
top level: BBa_E5504
access: public
definition: https://synbiohub.org/public/igem/BBa_B0032/1

BBa_R0040
type: Component
persistent identity: component1489160
display id: component1489160
version: 1
top level: BBa_E5504
access: public
definition: https://synbiohub.org/public/igem/BBa_R0040/1

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type: SequenceAnnotation
persistent identity: annotation1489160
display id: annotation1489160
version: 1
top level: BBa_E5504
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component: BBa_R0040

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persistent identity: range1489160
display id: range1489160
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display id: annotation1489171
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component: BBa_B0032

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display id: annotation1489174
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persistent identity: range1489174
display id: range1489174
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direction: direction/forward
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BBa_I12052
type: ComponentDefinition
persistent identity: BBa I12052
display id: BBa_I12052
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Test circuit for I12036
creator: endelman
created: 2004-09-02T11:00:00Z
modified: 2015-08-31T04:07:31Z
discontinued: false
dominant: false
experience: experience/None
group u list: _3_
m user id: 0
owner id: 145
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: IPTG and arabinose are used to control expression of lambda cI/LVA and 434 cI, respectively. These two repressors bind into I12036 and modulate expression of YFP/AAV. Must be used with LacIq strain.
mutable notes: This part links together generators for lambda cI and 434 cI with I12036 and YFP reporter.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_I12036
component: BBa_E0434
component: BBa_I12050
component: BBa_I12024
sequence annotation: BBa_I12050
sequence annotation: BBa_I12024
sequence annotation: BBa_I12036
sequence annotation: BBa_E0434
sequence: https://synbiohub.org/public/igem/BBa_I12052_sequence/1

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type: Component
persistent identity: component2235096
display id: component2235096
version: 1
top level: BBa_I12052
access: public
definition: https://synbiohub.org/public/igem/BBa_I12036/1

BBa_E0434
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persistent identity: component2235106
display id: component2235106
version: 1
top level: BBa_I12052
access: public
definition: https://synbiohub.org/public/igem/BBa_E0434/1

BBa_I12050
type: Component
persistent identity: component2235088
display id: component2235088
version: 1
top level: BBa_I12052
access: public
definition: https://synbiohub.org/public/igem/BBa_I12050/1

BBa_I12024
type: Component
persistent identity: component2235075
display id: component2235075
version: 1
top level: BBa_I12052
access: public
definition: https://synbiohub.org/public/igem/BBa_I12024/1

BBa_I12050
type: SequenceAnnotation
persistent identity: annotation2235088
display id: annotation2235088
version: 1
top level: BBa_I12052
location: https://synbiohub.org/public/igem/BBa_I12052/annotation2235088/range2235088/1
component: BBa_I12050

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persistent identity: range2235088
display id: range2235088
version: 1
direction: direction/reverse
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start: 1002
end: 3010
orientation: inline

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version: 1
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component: BBa_I12024

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persistent identity: range2235075
display id: range2235075
version: 1
direction: direction/reverse
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type: SequenceAnnotation
persistent identity: annotation2235096
display id: annotation2235096
version: 1
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location: https://synbiohub.org/public/igem/BBa_I12052/annotation2235096/range2235096/1
component: BBa_I12036

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persistent identity: range2235106
display id: range2235106
version: 1
direction: direction/reverse
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start: 3118
end: 4034
orientation: inline

BBa_Q03511
type: ComponentDefinition
persistent identity: BBa Q03511
display id: BBa_Q03511
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: QPI (B0033.C0051.B0015.R0065)
creator: Caitlin Conboy and Jennifer Braff
created: 2003-12-02T12:00:00Z
modified: 2015-05-08T01:14:13Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_R0065
component: BBa_C0051
component: BBa_B0033
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0033
sequence annotation: BBa_R0065
sequence annotation: BBa_B0010
sequence annotation: BBa_C0051
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_Q03511_sequence/1

BBa_R0065
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persistent identity: component952508
display id: component952508
version: 1
top level: BBa_Q03511
access: public
definition: https://synbiohub.org/public/igem/BBa_R0065/1

BBa_C0051
type: Component
persistent identity: component952472
display id: component952472
version: 1
top level: BBa_Q03511
access: public
definition: https://synbiohub.org/public/igem/BBa_C0051/1

BBa_B0033
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persistent identity: component952461
display id: component952461
version: 1
top level: BBa_Q03511
access: public
definition: https://synbiohub.org/public/igem/BBa_B0033/1

BBa_B0012
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persistent identity: component952488
display id: component952488
version: 1
top level: BBa_Q03511
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0010
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persistent identity: component952478
display id: component952478
version: 1
top level: BBa_Q03511
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

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persistent identity: annotation952461
display id: annotation952461
version: 1
top level: BBa_Q03511
location: https://synbiohub.org/public/igem/BBa_Q03511/annotation952461/range952461/1
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persistent identity: range952461
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display id: annotation952508
version: 1
top level: BBa_Q03511
location: https://synbiohub.org/public/igem/BBa_Q03511/annotation952508/range952508/1
component: BBa_R0065

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display id: range952508
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orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation952478
display id: annotation952478
version: 1
top level: BBa_Q03511
location: https://synbiohub.org/public/igem/BBa_Q03511/annotation952478/range952478/1
component: BBa_B0010

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display id: range952478
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direction: direction/forward
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start: 801
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persistent identity: annotation952472
display id: annotation952472
version: 1
top level: BBa_Q03511
location: https://synbiohub.org/public/igem/BBa_Q03511/annotation952472/range952472/1
component: BBa_C0051

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persistent identity: range952472
display id: range952472
version: 1
direction: direction/forward
top level: BBa_Q03511
start: 18
end: 767
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation952488
display id: annotation952488
version: 1
top level: BBa_Q03511
location: https://synbiohub.org/public/igem/BBa_Q03511/annotation952488/range952488/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_Q03511/annotation952488/range952488/1
type: Range
persistent identity: range952488
display id: range952488
version: 1
direction: direction/forward
top level: BBa_Q03511
start: 889
end: 929
orientation: inline

BBa_I14043
type: ComponentDefinition
persistent identity: BBa I14043
display id: BBa_I14043
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: medium RBS . LacI
creator: Aditi Shrivastava, Jiwon Lee, Sarah Welch
created: 2004-08-03T11:00:00Z
modified: 2015-08-31T04:07:38Z
discontinued: false
dominant: false
experience: experience/None
group u list: _4_
m user id: 0
owner id: 166
owning group id: 7
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: BBa_B0032 BBa_C0012
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_C0012
component: BBa_B0032
sequence annotation: BBa_C0012
sequence annotation: BBa_B0032
sequence: https://synbiohub.org/public/igem/BBa_I14043_sequence/1

BBa_C0012
type: Component
persistent identity: component1024237
display id: component1024237
version: 1
top level: BBa_I14043
access: public
definition: https://synbiohub.org/public/igem/BBa_C0012/1

BBa_B0032
type: Component
persistent identity: component1024226
display id: component1024226
version: 1
top level: BBa_I14043
access: public
definition: https://synbiohub.org/public/igem/BBa_B0032/1

BBa_C0012
type: SequenceAnnotation
persistent identity: annotation1024237
display id: annotation1024237
version: 1
top level: BBa_I14043
location: https://synbiohub.org/public/igem/BBa_I14043/annotation1024237/range1024237/1
component: BBa_C0012

https://synbiohub.org/public/igem/BBa_I14043/annotation1024237/range1024237/1
type: Range
persistent identity: range1024237
display id: range1024237
version: 1
direction: direction/forward
top level: BBa_I14043
start: 20
end: 1147
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation1024226
display id: annotation1024226
version: 1
top level: BBa_I14043
location: https://synbiohub.org/public/igem/BBa_I14043/annotation1024226/range1024226/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_I14043/annotation1024226/range1024226/1
type: Range
persistent identity: range1024226
display id: range1024226
version: 1
direction: direction/forward
top level: BBa_I14043
start: 1
end: 13
orientation: inline

BBa_I13410
type: ComponentDefinition
persistent identity: BBa I13410
display id: BBa_I13410
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Screening Plasmid Test Construct v2
creator: jasonk
created: 2005-04-28T11:00:00Z
modified: 2015-08-31T04:07:34Z
discontinued: false
dominant: false
experience: experience/None
group u list: _6_
m user id: 0
owner id: 2
owning group id: 6
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This is an example of the 2nd version of the screening plasmid test. In this version there is only one copy of pBAD and the POPSin reads-through a CFP coding region before reaching the device.
mutable notes: Has a deletion of the C at base 1194 (at the end of I0500). This is after the transcription start of the Pbad promoter and before the RBS of the transcript, so it should not be significant.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0012
component: BBa_I13411
component: BBa_B0010
component: BBa_I0500
component: BBa_E0030
component: BBa_B0032
sequence annotation: BBa_B0010
sequence annotation: BBa_B0032
sequence annotation: BBa_I0500
sequence annotation: BBa_I13411
sequence annotation: BBa_B0012
sequence annotation: BBa_E0030
sequence: https://synbiohub.org/public/igem/BBa_I13410_sequence/1

BBa_B0012
type: Component
persistent identity: component1490721
display id: component1490721
version: 1
top level: BBa_I13410
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_I13411
type: Component
persistent identity: component1490695
display id: component1490695
version: 1
top level: BBa_I13410
access: public
definition: https://synbiohub.org/public/igem/BBa_I13411/1

BBa_B0010
type: Component
persistent identity: component1490711
display id: component1490711
version: 1
top level: BBa_I13410
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_I0500
type: Component
persistent identity: component1490687
display id: component1490687
version: 1
top level: BBa_I13410
access: public
definition: https://synbiohub.org/public/igem/BBa_I0500/1

BBa_E0030
type: Component
persistent identity: component1490706
display id: component1490706
version: 1
top level: BBa_I13410
access: public
definition: https://synbiohub.org/public/igem/BBa_E0030/1

BBa_B0032
type: Component
persistent identity: component1490703
display id: component1490703
version: 1
top level: BBa_I13410
access: public
definition: https://synbiohub.org/public/igem/BBa_B0032/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1490711
display id: annotation1490711
version: 1
top level: BBa_I13410
location: https://synbiohub.org/public/igem/BBa_I13410/annotation1490711/range1490711/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I13410/annotation1490711/range1490711/1
type: Range
persistent identity: range1490711
display id: range1490711
version: 1
direction: direction/forward
top level: BBa_I13410
start: 2735
end: 2814
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation1490703
display id: annotation1490703
version: 1
top level: BBa_I13410
location: https://synbiohub.org/public/igem/BBa_I13410/annotation1490703/range1490703/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_I13410/annotation1490703/range1490703/1
type: Range
persistent identity: range1490703
display id: range1490703
version: 1
direction: direction/forward
top level: BBa_I13410
start: 1985
end: 1997
orientation: inline

BBa_I0500
type: SequenceAnnotation
persistent identity: annotation1490687
display id: annotation1490687
version: 1
top level: BBa_I13410
location: https://synbiohub.org/public/igem/BBa_I13410/annotation1490687/range1490687/1
component: BBa_I0500

https://synbiohub.org/public/igem/BBa_I13410/annotation1490687/range1490687/1
type: Range
persistent identity: range1490687
display id: range1490687
version: 1
direction: direction/forward
top level: BBa_I13410
start: 1
end: 1210
orientation: inline

BBa_I13411
type: SequenceAnnotation
persistent identity: annotation1490695
display id: annotation1490695
version: 1
top level: BBa_I13410
location: https://synbiohub.org/public/igem/BBa_I13410/annotation1490695/range1490695/1
component: BBa_I13411

https://synbiohub.org/public/igem/BBa_I13410/annotation1490695/range1490695/1
type: Range
persistent identity: range1490695
display id: range1490695
version: 1
direction: direction/forward
top level: BBa_I13410
start: 1219
end: 1976
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1490721
display id: annotation1490721
version: 1
top level: BBa_I13410
location: https://synbiohub.org/public/igem/BBa_I13410/annotation1490721/range1490721/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I13410/annotation1490721/range1490721/1
type: Range
persistent identity: range1490721
display id: range1490721
version: 1
direction: direction/forward
top level: BBa_I13410
start: 2823
end: 2863
orientation: inline

BBa_E0030
type: SequenceAnnotation
persistent identity: annotation1490706
display id: annotation1490706
version: 1
top level: BBa_I13410
location: https://synbiohub.org/public/igem/BBa_I13410/annotation1490706/range1490706/1
component: BBa_E0030

https://synbiohub.org/public/igem/BBa_I13410/annotation1490706/range1490706/1
type: Range
persistent identity: range1490706
display id: range1490706
version: 1
direction: direction/forward
top level: BBa_I13410
start: 2004
end: 2726
orientation: inline

BBa_I14031
type: ComponentDefinition
persistent identity: BBa I14031
display id: BBa_I14031
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Constitutive aiiA & dsRed
creator: Vikram Vijayan, Allen Hsu, Lawrence Fomundam
created: 2004-08-02T11:00:00Z
modified: 2015-08-31T04:07:37Z
discontinued: false
dominant: false
experience: experience/None
group u list: _4_
m user id: 0
owner id: 171
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: p(Bla), Strong RBS, aiiA + LVA, Strong RBS, dsRed - LVA
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_I14018
component: BBa_B0030
component: BBa_C0060
component: BBa_E0010
sequence annotation: BBa_B0030
sequence annotation: BBa_C0060
sequence annotation: BBa_E0010
sequence annotation: BBa_I14018
sequence: https://synbiohub.org/public/igem/BBa_I14031_sequence/1

BBa_I14018
type: Component
persistent identity: component1028921
display id: component1028921
version: 1
top level: BBa_I14031
access: public
definition: https://synbiohub.org/public/igem/BBa_I14018/1

BBa_B0030
type: Component
persistent identity: component1028956
display id: component1028956
version: 1
top level: BBa_I14031
access: public
definition: https://synbiohub.org/public/igem/BBa_B0030/1

BBa_C0060
type: Component
persistent identity: component1028945
display id: component1028945
version: 1
top level: BBa_I14031
access: public
definition: https://synbiohub.org/public/igem/BBa_C0060/1

BBa_B0030
type: Component
persistent identity: component1028929
display id: component1028929
version: 1
top level: BBa_I14031
access: public
definition: https://synbiohub.org/public/igem/BBa_B0030/1

BBa_E0010
type: Component
persistent identity: component1028959
display id: component1028959
version: 1
top level: BBa_I14031
access: public
definition: https://synbiohub.org/public/igem/BBa_E0010/1

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation1028929
display id: annotation1028929
version: 1
top level: BBa_I14031
location: https://synbiohub.org/public/igem/BBa_I14031/annotation1028929/range1028929/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_I14031/annotation1028929/range1028929/1
type: Range
persistent identity: range1028929
display id: range1028929
version: 1
direction: direction/forward
top level: BBa_I14031
start: 44
end: 58
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation1028956
display id: annotation1028956
version: 1
top level: BBa_I14031
location: https://synbiohub.org/public/igem/BBa_I14031/annotation1028956/range1028956/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_I14031/annotation1028956/range1028956/1
type: Range
persistent identity: range1028956
display id: range1028956
version: 1
direction: direction/forward
top level: BBa_I14031
start: 887
end: 901
orientation: inline

BBa_C0060
type: SequenceAnnotation
persistent identity: annotation1028945
display id: annotation1028945
version: 1
top level: BBa_I14031
location: https://synbiohub.org/public/igem/BBa_I14031/annotation1028945/range1028945/1
component: BBa_C0060

https://synbiohub.org/public/igem/BBa_I14031/annotation1028945/range1028945/1
type: Range
persistent identity: range1028945
display id: range1028945
version: 1
direction: direction/forward
top level: BBa_I14031
start: 65
end: 853
orientation: inline

BBa_E0010
type: SequenceAnnotation
persistent identity: annotation1028959
display id: annotation1028959
version: 1
top level: BBa_I14031
location: https://synbiohub.org/public/igem/BBa_I14031/annotation1028959/range1028959/1
component: BBa_E0010

https://synbiohub.org/public/igem/BBa_I14031/annotation1028959/range1028959/1
type: Range
persistent identity: range1028959
display id: range1028959
version: 1
direction: direction/forward
top level: BBa_I14031
start: 908
end: 1588
orientation: inline

BBa_I14018
type: SequenceAnnotation
persistent identity: annotation1028921
display id: annotation1028921
version: 1
top level: BBa_I14031
location: https://synbiohub.org/public/igem/BBa_I14031/annotation1028921/range1028921/1
component: BBa_I14018

https://synbiohub.org/public/igem/BBa_I14031/annotation1028921/range1028921/1
type: Range
persistent identity: range1028921
display id: range1028921
version: 1
direction: direction/forward
top level: BBa_I14031
start: 1
end: 35
orientation: inline

BBa_I13312
type: ComponentDefinition
persistent identity: BBa I13312
display id: BBa_I13312
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: I13306.E0430 Tet-Regulated Las Amplifier with YFP Reporter
creator: Barry and Debra and Jay
created: 2004-08-08T11:00:00Z
modified: 2015-08-31T04:07:33Z
discontinued: false
dominant: false
experience: experience/None
group u list: _6_
m user id: 0
owner id: 101
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_R0040
component: BBa_C0179
component: BBa_R0079
component: BBa_B0010
component: BBa_B0012
component: BBa_E0030
component: BBa_B0034
sequence annotation: BBa_R0079
sequence annotation: BBa_E0030
sequence annotation: BBa_B0034
sequence annotation: BBa_B0012
sequence annotation: BBa_R0040
sequence annotation: BBa_C0179
sequence annotation: BBa_B0010
sequence: https://synbiohub.org/public/igem/BBa_I13312_sequence/1

BBa_R0040
type: Component
persistent identity: component1036849
display id: component1036849
version: 1
top level: BBa_I13312
access: public
definition: https://synbiohub.org/public/igem/BBa_R0040/1

BBa_C0179
type: Component
persistent identity: component1036859
display id: component1036859
version: 1
top level: BBa_I13312
access: public
definition: https://synbiohub.org/public/igem/BBa_C0179/1

BBa_R0079
type: Component
persistent identity: component1036891
display id: component1036891
version: 1
top level: BBa_I13312
access: public
definition: https://synbiohub.org/public/igem/BBa_R0079/1

BBa_B0010
type: Component
persistent identity: component1036903
display id: component1036903
version: 1
top level: BBa_I13312
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0012
type: Component
persistent identity: component1036913
display id: component1036913
version: 1
top level: BBa_I13312
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_E0030
type: Component
persistent identity: component1036898
display id: component1036898
version: 1
top level: BBa_I13312
access: public
definition: https://synbiohub.org/public/igem/BBa_E0030/1

BBa_B0034
type: Component
persistent identity: component1036896
display id: component1036896
version: 1
top level: BBa_I13312
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0034
type: Component
persistent identity: component1036857
display id: component1036857
version: 1
top level: BBa_I13312
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0010
type: Component
persistent identity: component1036864
display id: component1036864
version: 1
top level: BBa_I13312
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0012
type: Component
persistent identity: component1036874
display id: component1036874
version: 1
top level: BBa_I13312
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_R0079
type: SequenceAnnotation
persistent identity: annotation1036891
display id: annotation1036891
version: 1
top level: BBa_I13312
location: https://synbiohub.org/public/igem/BBa_I13312/annotation1036891/range1036891/1
component: BBa_R0079

https://synbiohub.org/public/igem/BBa_I13312/annotation1036891/range1036891/1
type: Range
persistent identity: range1036891
display id: range1036891
version: 1
direction: direction/forward
top level: BBa_I13312
start: 949
end: 1105
orientation: inline

BBa_E0030
type: SequenceAnnotation
persistent identity: annotation1036898
display id: annotation1036898
version: 1
top level: BBa_I13312
location: https://synbiohub.org/public/igem/BBa_I13312/annotation1036898/range1036898/1
component: BBa_E0030

https://synbiohub.org/public/igem/BBa_I13312/annotation1036898/range1036898/1
type: Range
persistent identity: range1036898
display id: range1036898
version: 1
direction: direction/forward
top level: BBa_I13312
start: 1132
end: 1854
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1036857
display id: annotation1036857
version: 1
top level: BBa_I13312
location: https://synbiohub.org/public/igem/BBa_I13312/annotation1036857/range1036857/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I13312/annotation1036857/range1036857/1
type: Range
persistent identity: range1036857
display id: range1036857
version: 1
direction: direction/forward
top level: BBa_I13312
start: 63
end: 74
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1036913
display id: annotation1036913
version: 1
top level: BBa_I13312
location: https://synbiohub.org/public/igem/BBa_I13312/annotation1036913/range1036913/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I13312/annotation1036913/range1036913/1
type: Range
persistent identity: range1036913
display id: range1036913
version: 1
direction: direction/forward
top level: BBa_I13312
start: 1951
end: 1991
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1036896
display id: annotation1036896
version: 1
top level: BBa_I13312
location: https://synbiohub.org/public/igem/BBa_I13312/annotation1036896/range1036896/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I13312/annotation1036896/range1036896/1
type: Range
persistent identity: range1036896
display id: range1036896
version: 1
direction: direction/forward
top level: BBa_I13312
start: 1114
end: 1125
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1036849
display id: annotation1036849
version: 1
top level: BBa_I13312
location: https://synbiohub.org/public/igem/BBa_I13312/annotation1036849/range1036849/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_I13312/annotation1036849/range1036849/1
type: Range
persistent identity: range1036849
display id: range1036849
version: 1
direction: direction/forward
top level: BBa_I13312
start: 1
end: 54
orientation: inline

BBa_C0179
type: SequenceAnnotation
persistent identity: annotation1036859
display id: annotation1036859
version: 1
top level: BBa_I13312
location: https://synbiohub.org/public/igem/BBa_I13312/annotation1036859/range1036859/1
component: BBa_C0179

https://synbiohub.org/public/igem/BBa_I13312/annotation1036859/range1036859/1
type: Range
persistent identity: range1036859
display id: range1036859
version: 1
direction: direction/forward
top level: BBa_I13312
start: 81
end: 803
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1036874
display id: annotation1036874
version: 1
top level: BBa_I13312
location: https://synbiohub.org/public/igem/BBa_I13312/annotation1036874/range1036874/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I13312/annotation1036874/range1036874/1
type: Range
persistent identity: range1036874
display id: range1036874
version: 1
direction: direction/forward
top level: BBa_I13312
start: 900
end: 940
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1036864
display id: annotation1036864
version: 1
top level: BBa_I13312
location: https://synbiohub.org/public/igem/BBa_I13312/annotation1036864/range1036864/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I13312/annotation1036864/range1036864/1
type: Range
persistent identity: range1036864
display id: range1036864
version: 1
direction: direction/forward
top level: BBa_I13312
start: 812
end: 891
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1036903
display id: annotation1036903
version: 1
top level: BBa_I13312
location: https://synbiohub.org/public/igem/BBa_I13312/annotation1036903/range1036903/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I13312/annotation1036903/range1036903/1
type: Range
persistent identity: range1036903
display id: range1036903
version: 1
direction: direction/forward
top level: BBa_I13312
start: 1863
end: 1942
orientation: inline

BBa_I12002
type: ComponentDefinition
persistent identity: BBa I12002
display id: BBa_I12002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Barkai-Leibler design experiment part B (434cI)
creator: Hans
created: 2004-07-12T11:00:00Z
modified: 2015-08-31T04:07:31Z
discontinued: false
dominant: false
experience: experience/None
group u list: _3_
m user id: 0
owner id: 147
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This forms the repressor of a Barkai-Leibler oscillator. The 434 cI has no LVA tag (BBa_I12014 is the same as BBa_C0052 with no LVA tag).
mutable notes: This part to work in conjunction with BBa_I12000. This part creates one of the proteins involved in the Modified Barkai-Leibler relaxation oscillator design (repressor protein: 434 cI). In the presence of Lambda cI, this part will create 434 cI, which will in turn repress the production of Lambda cI in BBa_12000. The LVA tag was removed from the 434 cI coding region due to the fact that we want the repressor to be as potent as possible, thus, if it doesn't degrade as quickly, the relative strength should be increased.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_I12014
component: BBa_B0025
component: BBa_I12007
component: BBa_B0015
component: BBa_B0034
sequence annotation: BBa_B0025
sequence annotation: BBa_B0015
sequence annotation: BBa_I12007
sequence annotation: BBa_I12014
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_I12002_sequence/1

BBa_I12014
type: Component
persistent identity: component2223483
display id: component2223483
version: 1
top level: BBa_I12002
access: public
definition: https://synbiohub.org/public/igem/BBa_I12014/1

BBa_B0025
type: Component
persistent identity: component2223473
display id: component2223473
version: 1
top level: BBa_I12002
access: public
definition: https://synbiohub.org/public/igem/BBa_B0025/1

BBa_I12007
type: Component
persistent identity: component2223479
display id: component2223479
version: 1
top level: BBa_I12002
access: public
definition: https://synbiohub.org/public/igem/BBa_I12007/1

BBa_B0015
type: Component
persistent identity: component2223490
display id: component2223490
version: 1
top level: BBa_I12002
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_B0034
type: Component
persistent identity: component2223481
display id: component2223481
version: 1
top level: BBa_I12002
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0025
type: SequenceAnnotation
persistent identity: annotation2223473
display id: annotation2223473
version: 1
top level: BBa_I12002
location: https://synbiohub.org/public/igem/BBa_I12002/annotation2223473/range2223473/1
component: BBa_B0025

https://synbiohub.org/public/igem/BBa_I12002/annotation2223473/range2223473/1
type: Range
persistent identity: range2223473
display id: range2223473
version: 1
direction: direction/reverse
top level: BBa_I12002
start: 1
end: 129
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2223490
display id: annotation2223490
version: 1
top level: BBa_I12002
location: https://synbiohub.org/public/igem/BBa_I12002/annotation2223490/range2223490/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_I12002/annotation2223490/range2223490/1
type: Range
persistent identity: range2223490
display id: range2223490
version: 1
direction: direction/reverse
top level: BBa_I12002
start: 890
end: 1018
orientation: inline

BBa_I12007
type: SequenceAnnotation
persistent identity: annotation2223479
display id: annotation2223479
version: 1
top level: BBa_I12002
location: https://synbiohub.org/public/igem/BBa_I12002/annotation2223479/range2223479/1
component: BBa_I12007

https://synbiohub.org/public/igem/BBa_I12002/annotation2223479/range2223479/1
type: Range
persistent identity: range2223479
display id: range2223479
version: 1
direction: direction/reverse
top level: BBa_I12002
start: 138
end: 219
orientation: inline

BBa_I12014
type: SequenceAnnotation
persistent identity: annotation2223483
display id: annotation2223483
version: 1
top level: BBa_I12002
location: https://synbiohub.org/public/igem/BBa_I12002/annotation2223483/range2223483/1
component: BBa_I12014

https://synbiohub.org/public/igem/BBa_I12002/annotation2223483/range2223483/1
type: Range
persistent identity: range2223483
display id: range2223483
version: 1
direction: direction/reverse
top level: BBa_I12002
start: 246
end: 881
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2223481
display id: annotation2223481
version: 1
top level: BBa_I12002
location: https://synbiohub.org/public/igem/BBa_I12002/annotation2223481/range2223481/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I12002/annotation2223481/range2223481/1
type: Range
persistent identity: range2223481
display id: range2223481
version: 1
direction: direction/reverse
top level: BBa_I12002
start: 228
end: 239
orientation: inline

BBa_I13303
type: ComponentDefinition
persistent identity: BBa I13303
display id: BBa_I13303
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: R0011.I13271 Lac-Regulated Rhl Amplifier
creator: Jay and Debra and Barry
created: 2004-08-08T11:00:00Z
modified: 2015-08-31T04:07:33Z
discontinued: false
dominant: false
experience: experience/None
group u list: _6_
m user id: 0
owner id: 101
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_R0011
component: BBa_I13271
sequence annotation: BBa_R0011
sequence annotation: BBa_I13271
sequence: https://synbiohub.org/public/igem/BBa_I13303_sequence/1

BBa_R0011
type: Component
persistent identity: component2223534
display id: component2223534
version: 1
top level: BBa_I13303
access: public
definition: https://synbiohub.org/public/igem/BBa_R0011/1

BBa_I13271
type: Component
persistent identity: component2223555
display id: component2223555
version: 1
top level: BBa_I13303
access: public
definition: BBa_I13271

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation2223534
display id: annotation2223534
version: 1
top level: BBa_I13303
location: https://synbiohub.org/public/igem/BBa_I13303/annotation2223534/range2223534/1
component: BBa_R0011

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type: Range
persistent identity: range2223534
display id: range2223534
version: 1
direction: direction/forward
top level: BBa_I13303
start: 1
end: 54
orientation: inline

BBa_I13271
type: SequenceAnnotation
persistent identity: annotation2223555
display id: annotation2223555
version: 1
top level: BBa_I13303
location: https://synbiohub.org/public/igem/BBa_I13303/annotation2223555/range2223555/1
component: BBa_I13271

https://synbiohub.org/public/igem/BBa_I13303/annotation2223555/range2223555/1
type: Range
persistent identity: range2223555
display id: range2223555
version: 1
direction: direction/reverse
top level: BBa_I13303
start: 64
end: 1066
orientation: inline

BBa_I12053
type: ComponentDefinition
persistent identity: BBa I12053
display id: BBa_I12053
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Test circuit for I12040
creator: endelman
created: 2004-09-02T11:00:00Z
modified: 2015-08-31T04:07:31Z
discontinued: false
dominant: false
experience: experience/None
group u list: _3_
m user id: 0
owner id: 145
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: IPTG and arabinose are used to control expression of lambda cI/LVA and 434 cI, respectively. These two repressors bind into I12040 and modulate expression of YFP/AAV. Must be used with LacIq strain.
mutable notes: This part links together generators for lambda cI and 434 cI with I12040 and YFP reporter.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_E0434
component: BBa_I12024
component: BBa_I12040
component: BBa_I12050
sequence annotation: BBa_E0434
sequence annotation: BBa_I12050
sequence annotation: BBa_I12040
sequence annotation: BBa_I12024
sequence: https://synbiohub.org/public/igem/BBa_I12053_sequence/1

BBa_E0434
type: Component
persistent identity: component2235156
display id: component2235156
version: 1
top level: BBa_I12053
access: public
definition: https://synbiohub.org/public/igem/BBa_E0434/1

BBa_I12024
type: Component
persistent identity: component2235125
display id: component2235125
version: 1
top level: BBa_I12053
access: public
definition: https://synbiohub.org/public/igem/BBa_I12024/1

BBa_I12040
type: Component
persistent identity: component2235146
display id: component2235146
version: 1
top level: BBa_I12053
access: public
definition: https://synbiohub.org/public/igem/BBa_I12040/1

BBa_I12050
type: Component
persistent identity: component2235138
display id: component2235138
version: 1
top level: BBa_I12053
access: public
definition: https://synbiohub.org/public/igem/BBa_I12050/1

BBa_E0434
type: SequenceAnnotation
persistent identity: annotation2235156
display id: annotation2235156
version: 1
top level: BBa_I12053
location: https://synbiohub.org/public/igem/BBa_I12053/annotation2235156/range2235156/1
component: BBa_E0434

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type: Range
persistent identity: range2235156
display id: range2235156
version: 1
direction: direction/reverse
top level: BBa_I12053
start: 3118
end: 4034
orientation: inline

BBa_I12050
type: SequenceAnnotation
persistent identity: annotation2235138
display id: annotation2235138
version: 1
top level: BBa_I12053
location: https://synbiohub.org/public/igem/BBa_I12053/annotation2235138/range2235138/1
component: BBa_I12050

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type: Range
persistent identity: range2235138
display id: range2235138
version: 1
direction: direction/reverse
top level: BBa_I12053
start: 1002
end: 3010
orientation: inline

BBa_I12040
type: SequenceAnnotation
persistent identity: annotation2235146
display id: annotation2235146
version: 1
top level: BBa_I12053
location: https://synbiohub.org/public/igem/BBa_I12053/annotation2235146/range2235146/1
component: BBa_I12040

https://synbiohub.org/public/igem/BBa_I12053/annotation2235146/range2235146/1
type: Range
persistent identity: range2235146
display id: range2235146
version: 1
direction: direction/reverse
top level: BBa_I12053
start: 3019
end: 3109
orientation: inline

BBa_I12024
type: SequenceAnnotation
persistent identity: annotation2235125
display id: annotation2235125
version: 1
top level: BBa_I12053
location: https://synbiohub.org/public/igem/BBa_I12053/annotation2235125/range2235125/1
component: BBa_I12024

https://synbiohub.org/public/igem/BBa_I12053/annotation2235125/range2235125/1
type: Range
persistent identity: range2235125
display id: range2235125
version: 1
direction: direction/reverse
top level: BBa_I12053
start: 1
end: 993
orientation: inline

BBa_I5310
type: ComponentDefinition
persistent identity: BBa I5310
display id: BBa_I5310
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Olmecs: Reporter 2 of Synchro-Dualator
creator: Olmecs 2003 IAP Team
created: 2003-11-27T12:00:00Z
modified: 2015-08-31T04:07:42Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: For reference only
status: status/Informational
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0011
component: BBa_B0034
component: BBa_R0011
component: BBa_E0032
component: BBa_B0012
sequence annotation: BBa_B0034
sequence annotation: BBa_B0012
sequence annotation: BBa_B0011
sequence annotation: BBa_R0011
sequence annotation: BBa_E0032
sequence: https://synbiohub.org/public/igem/BBa_I5310_sequence/1

BBa_B0011
type: Component
persistent identity: component2221060
display id: component2221060
version: 1
top level: BBa_I5310
access: public
definition: https://synbiohub.org/public/igem/BBa_B0011/1

BBa_B0034
type: Component
persistent identity: component2221050
display id: component2221050
version: 1
top level: BBa_I5310
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_R0011
type: Component
persistent identity: component2221044
display id: component2221044
version: 1
top level: BBa_I5310
access: public
definition: https://synbiohub.org/public/igem/BBa_R0011/1

BBa_E0032
type: Component
persistent identity: component2221052
display id: component2221052
version: 1
top level: BBa_I5310
access: public
definition: https://synbiohub.org/public/igem/BBa_E0032/1

BBa_B0012
type: Component
persistent identity: component2221056
display id: component2221056
version: 1
top level: BBa_I5310
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2221050
display id: annotation2221050
version: 1
top level: BBa_I5310
location: https://synbiohub.org/public/igem/BBa_I5310/annotation2221050/range2221050/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I5310/annotation2221050/range2221050/1
type: Range
persistent identity: range2221050
display id: range2221050
version: 1
direction: direction/reverse
top level: BBa_I5310
start: 64
end: 75
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2221056
display id: annotation2221056
version: 1
top level: BBa_I5310
location: https://synbiohub.org/public/igem/BBa_I5310/annotation2221056/range2221056/1
component: BBa_B0012

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type: Range
persistent identity: range2221056
display id: range2221056
version: 1
direction: direction/forward
top level: BBa_I5310
start: 852
end: 892
orientation: inline

BBa_B0011
type: SequenceAnnotation
persistent identity: annotation2221060
display id: annotation2221060
version: 1
top level: BBa_I5310
location: https://synbiohub.org/public/igem/BBa_I5310/annotation2221060/range2221060/1
component: BBa_B0011

https://synbiohub.org/public/igem/BBa_I5310/annotation2221060/range2221060/1
type: Range
persistent identity: range2221060
display id: range2221060
version: 1
direction: direction/forward
top level: BBa_I5310
start: 901
end: 946
orientation: inline

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation2221044
display id: annotation2221044
version: 1
top level: BBa_I5310
location: https://synbiohub.org/public/igem/BBa_I5310/annotation2221044/range2221044/1
component: BBa_R0011

https://synbiohub.org/public/igem/BBa_I5310/annotation2221044/range2221044/1
type: Range
persistent identity: range2221044
display id: range2221044
version: 1
direction: direction/forward
top level: BBa_I5310
start: 1
end: 54
orientation: inline

BBa_E0032
type: SequenceAnnotation
persistent identity: annotation2221052
display id: annotation2221052
version: 1
top level: BBa_I5310
location: https://synbiohub.org/public/igem/BBa_I5310/annotation2221052/range2221052/1
component: BBa_E0032

https://synbiohub.org/public/igem/BBa_I5310/annotation2221052/range2221052/1
type: Range
persistent identity: range2221052
display id: range2221052
version: 1
direction: direction/forward
top level: BBa_I5310
start: 82
end: 843
orientation: inline

BBa_I14014
type: ComponentDefinition
persistent identity: BBa I14014
display id: BBa_I14014
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: R10, B30, C79
creator: Vikram Vijayan, Allen Hsu, Lawrence Fomundam
created: 2004-07-29T11:00:00Z
modified: 2015-08-31T04:07:37Z
discontinued: false
dominant: false
experience: experience/None
group u list: _4_
m user id: 0
owner id: 171
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: p(Lac) (R10), Strong RBS (B30), LasR+ LVA (C79)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0030
component: BBa_R0010
component: BBa_C0079
sequence annotation: BBa_C0079
sequence annotation: BBa_R0010
sequence annotation: BBa_B0030
sequence: https://synbiohub.org/public/igem/BBa_I14014_sequence/1

BBa_B0030
type: Component
persistent identity: component1015845
display id: component1015845
version: 1
top level: BBa_I14014
access: public
definition: https://synbiohub.org/public/igem/BBa_B0030/1

BBa_R0010
type: Component
persistent identity: component1015832
display id: component1015832
version: 1
top level: BBa_I14014
access: public
definition: https://synbiohub.org/public/igem/BBa_R0010/1

BBa_C0079
type: Component
persistent identity: component1015859
display id: component1015859
version: 1
top level: BBa_I14014
access: public
definition: https://synbiohub.org/public/igem/BBa_C0079/1

BBa_C0079
type: SequenceAnnotation
persistent identity: annotation1015859
display id: annotation1015859
version: 1
top level: BBa_I14014
location: https://synbiohub.org/public/igem/BBa_I14014/annotation1015859/range1015859/1
component: BBa_C0079

https://synbiohub.org/public/igem/BBa_I14014/annotation1015859/range1015859/1
type: Range
persistent identity: range1015859
display id: range1015859
version: 1
direction: direction/forward
top level: BBa_I14014
start: 230
end: 985
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation1015832
display id: annotation1015832
version: 1
top level: BBa_I14014
location: https://synbiohub.org/public/igem/BBa_I14014/annotation1015832/range1015832/1
component: BBa_R0010

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type: Range
persistent identity: range1015832
display id: range1015832
version: 1
direction: direction/forward
top level: BBa_I14014
start: 1
end: 200
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation1015845
display id: annotation1015845
version: 1
top level: BBa_I14014
location: https://synbiohub.org/public/igem/BBa_I14014/annotation1015845/range1015845/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_I14014/annotation1015845/range1015845/1
type: Range
persistent identity: range1015845
display id: range1015845
version: 1
direction: direction/forward
top level: BBa_I14014
start: 209
end: 223
orientation: inline

BBa_I14035
type: ComponentDefinition
persistent identity: BBa I14035
display id: BBa_I14035
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: C12, C4 (medium RBS)
creator: Vikram Vijayan, Allen Hsu, Lawrence Fomundam
created: 2004-08-03T11:00:00Z
modified: 2015-08-31T04:07:38Z
discontinued: false
dominant: false
experience: experience/None
group u list: _4_
m user id: 0
owner id: 171
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: p(Las) TetO, Medium RBS, RhlI + LVA
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_C0070
component: BBa_I14015
component: BBa_B0032
sequence annotation: BBa_B0032
sequence annotation: BBa_C0070
sequence annotation: BBa_I14015
sequence: https://synbiohub.org/public/igem/BBa_I14035_sequence/1

BBa_C0070
type: Component
persistent identity: component1029003
display id: component1029003
version: 1
top level: BBa_I14035
access: public
definition: https://synbiohub.org/public/igem/BBa_C0070/1

BBa_I14015
type: Component
persistent identity: component1028984
display id: component1028984
version: 1
top level: BBa_I14035
access: public
definition: https://synbiohub.org/public/igem/BBa_I14015/1

BBa_B0032
type: Component
persistent identity: component1028992
display id: component1028992
version: 1
top level: BBa_I14035
access: public
definition: https://synbiohub.org/public/igem/BBa_B0032/1

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation1028992
display id: annotation1028992
version: 1
top level: BBa_I14035
location: https://synbiohub.org/public/igem/BBa_I14035/annotation1028992/range1028992/1
component: BBa_B0032

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type: Range
persistent identity: range1028992
display id: range1028992
version: 1
direction: direction/forward
top level: BBa_I14035
start: 179
end: 191
orientation: inline

BBa_C0070
type: SequenceAnnotation
persistent identity: annotation1029003
display id: annotation1029003
version: 1
top level: BBa_I14035
location: https://synbiohub.org/public/igem/BBa_I14035/annotation1029003/range1029003/1
component: BBa_C0070

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type: Range
persistent identity: range1029003
display id: range1029003
version: 1
direction: direction/forward
top level: BBa_I14035
start: 198
end: 839
orientation: inline

BBa_I14015
type: SequenceAnnotation
persistent identity: annotation1028984
display id: annotation1028984
version: 1
top level: BBa_I14035
location: https://synbiohub.org/public/igem/BBa_I14035/annotation1028984/range1028984/1
component: BBa_I14015

https://synbiohub.org/public/igem/BBa_I14035/annotation1028984/range1028984/1
type: Range
persistent identity: range1028984
display id: range1028984
version: 1
direction: direction/forward
top level: BBa_I14035
start: 1
end: 170
orientation: inline

BBa_J05304
type: ComponentDefinition
persistent identity: BBa J05304
display id: BBa_J05304
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: R4 system A
creator: Alexander Roth
created: 2005-09-14T11:00:00Z
modified: 2015-08-31T04:08:15Z
discontinued: true
dominant: false
group u list: _34_
m user id: 0
owner id: 412
owning group id: 34
sample status: Discontinued
status: status/Deleted
bookmark: false
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was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Lambda cI857 (Lambda II) QPI regulated by 434 cI with EYFP
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modified: 2015-08-31T04:08:19Z
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description: 3OC12HSL receiver, CI repressor hybrid
creator: kxjin
created: 2005-06-20T11:00:00Z
modified: 2015-08-31T04:08:16Z
discontinued: false
dominant: false
experience: experience/None
group u list: _20_
m user id: 0
owner id: 368
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sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Converts a 3OC12HL signal to CI output.
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owned by: myers
star: false
type: DnaRegion
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was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Lambda cI857 (Lambda II) QPI regulated by 434 cI with mCherry
creator: ytwang
created: 2005-06-27T11:00:00Z
modified: 2015-08-31T04:08:19Z
discontinued: true
dominant: false
experience: experience/None
group u list: _20_
m user id: 0
owner id: 340
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sample status: Discontinued
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bookmark: false
mutable description: -- No description --
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BBa_B0010
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BBa_S04000
type: ComponentDefinition
persistent identity: BBa S04000
display id: BBa_S04000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Ptet . Las R Cassette
creator: CC Signalling
created: 2004-07-18T11:00:00Z
is replaced by: https://synbiohub.org/public/igem/BBa_S03119/1
modified: 2015-05-08T01:14:33Z
discontinued: true
dominant: false
experience: experience/None
group u list: _6_
m user id: 0
owner id: 101
owning group id: 7
part status: Released HQ 2013
sample status: Discontinued
status: status/Deleted
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0010
component: BBa_C0062
component: BBa_B0034
component: BBa_B0012
component: BBa_R0040
sequence annotation: BBa_B0010
sequence annotation: BBa_B0034
sequence annotation: BBa_R0040
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BBa_C0062
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BBa_K1379005
type: ComponentDefinition
persistent identity: BBa K1379005
display id: BBa_K1379005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: σx Generator + PcelA-E0240
creator: Jordy Evan, Nadia Benedicta, Angeline Widjaja
created: 2014-09-22T11:00:00Z
modified: 2015-05-08T01:10:12Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1756_
m user id: 0
owner id: 21235
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: SigmaX generator, SigmaX inducible PCelA encoding GFP
mutable notes: N/A
mutable provenance: Both comX gene and PCelA are found in genomic sequence of Streptococcus pneumoniae NCTC7465 strain, while the RBS+GFP+DTT which is downstream of PCelA is BBa_E0240. The promoter encoding comX is BBa_K880005 and the double terminator for it is BBa_B0015
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owned by: myers
star: false
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role: SO:0000804
role: partType/Measurement
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component: BBa_K1379000
component: BBa_K1379006
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sequence annotation: BBa_E0240
sequence annotation: BBa_K1379006
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type: ComponentDefinition
persistent identity: BBa J06000
display id: BBa_J06000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: AHL -> YFP Receiver Test Part
creator: sash31
created: 2005-06-12T11:00:00Z
modified: 2015-08-31T04:08:16Z
discontinued: false
dominant: false
experience: experience/None
group u list: _20_
m user id: 0
owner id: 339
owning group id: 20
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Should make YFP when given AHL. (Used to be a twin of I13272; revised 6/27 to use E0432 instead of E0434.)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0010
component: BBa_B0034
component: BBa_B0012
component: BBa_R0063
component: BBa_E0032
component: BBa_R0062
component: BBa_C0062
sequence annotation: BBa_B0010
sequence annotation: BBa_R0063
sequence annotation: BBa_B0034
sequence annotation: BBa_B0012
sequence annotation: BBa_R0062
sequence annotation: BBa_C0062
sequence annotation: BBa_E0032
sequence: https://synbiohub.org/public/igem/BBa_J06000_sequence/1

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persistent identity: component1540995
display id: component1540995
version: 1
top level: BBa_J06000
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

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display id: component1540919
version: 1
top level: BBa_J06000
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

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display id: component1541005
version: 1
top level: BBa_J06000
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0012
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persistent identity: component1540952
display id: component1540952
version: 1
top level: BBa_J06000
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

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display id: component1540942
version: 1
top level: BBa_J06000
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

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display id: component1540975
version: 1
top level: BBa_J06000
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

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display id: component1540910
version: 1
top level: BBa_J06000
access: public
definition: https://synbiohub.org/public/igem/BBa_R0063/1

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persistent identity: component1540987
display id: component1540987
version: 1
top level: BBa_J06000
access: public
definition: https://synbiohub.org/public/igem/BBa_E0032/1

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display id: component1540967
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access: public
definition: https://synbiohub.org/public/igem/BBa_R0062/1

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display id: component1540934
version: 1
top level: BBa_J06000
access: public
definition: https://synbiohub.org/public/igem/BBa_C0062/1

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orientation: inline

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persistent identity: range1540942
display id: range1540942
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direction: direction/forward
top level: BBa_J06000
start: 967
end: 1046
orientation: inline

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display id: range1541005
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end: 2083
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persistent identity: range1540934
display id: range1540934
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persistent identity: range1540987
display id: range1540987
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end: 1946
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type: ComponentDefinition
persistent identity: BBa J05311
display id: BBa_J05311
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: R1 state
creator: Alexander Roth
created: 2005-09-14T11:00:00Z
modified: 2015-08-31T04:08:15Z
discontinued: false
dominant: false
group u list: _34_
m user id: 0
owner id: 412
owning group id: 34
sample status: Not in stock
status: status/Unavailable
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0034
component: BBa_B0015
component: BBa_J05215
component: BBa_J05108
sequence annotation: BBa_B0015
sequence annotation: BBa_J05108
sequence annotation: BBa_B0034
sequence annotation: BBa_J05215
sequence: https://synbiohub.org/public/igem/BBa_J05311_sequence/1

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persistent identity: component2221731
display id: component2221731
version: 1
top level: BBa_J05311
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

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display id: component2221746
version: 1
top level: BBa_J05311
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

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display id: component2221729
version: 1
top level: BBa_J05311
access: public
definition: https://synbiohub.org/public/igem/BBa_J05215/1

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display id: component2221739
version: 1
top level: BBa_J05311
access: public
definition: https://synbiohub.org/public/igem/BBa_J05108/1

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display id: annotation2221746
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persistent identity: range2221739
display id: range2221739
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direction: direction/reverse
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orientation: inline

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type: SequenceAnnotation
persistent identity: annotation2221731
display id: annotation2221731
version: 1
top level: BBa_J05311
location: https://synbiohub.org/public/igem/BBa_J05311/annotation2221731/range2221731/1
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type: Range
persistent identity: range2221731
display id: range2221731
version: 1
direction: direction/reverse
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orientation: inline

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type: SequenceAnnotation
persistent identity: annotation2221729
display id: annotation2221729
version: 1
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location: https://synbiohub.org/public/igem/BBa_J05311/annotation2221729/range2221729/1
component: BBa_J05215

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type: Range
persistent identity: range2221729
display id: range2221729
version: 1
direction: direction/reverse
top level: BBa_J05311
start: 1
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orientation: inline

BBa_I14052
type: ComponentDefinition
persistent identity: BBa I14052
display id: BBa_I14052
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: C12, C12 Router (weak RBS)
creator: Vikram Vijayan, Allen Hsu, Lawrence Fomundam
created: 2004-08-03T11:00:00Z
modified: 2015-08-31T04:07:38Z
discontinued: false
dominant: false
experience: experience/None
group u list: _4_
m user id: 0
owner id: 171
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: p(Las) CI O, Weak RBS, LasI + LVA
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_C0078
component: BBa_B0031
component: BBa_I14016
sequence annotation: BBa_C0078
sequence annotation: BBa_I14016
sequence annotation: BBa_B0031
sequence: https://synbiohub.org/public/igem/BBa_I14052_sequence/1

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persistent identity: component1029238
display id: component1029238
version: 1
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access: public
definition: https://synbiohub.org/public/igem/BBa_C0078/1

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persistent identity: component1029228
display id: component1029228
version: 1
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access: public
definition: https://synbiohub.org/public/igem/BBa_B0031/1

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persistent identity: component1029223
display id: component1029223
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access: public
definition: https://synbiohub.org/public/igem/BBa_I14016/1

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persistent identity: annotation1029238
display id: annotation1029238
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persistent identity: range1029238
display id: range1029238
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direction: direction/forward
top level: BBa_I14052
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end: 838
orientation: inline

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persistent identity: annotation1029228
display id: annotation1029228
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persistent identity: range1029228
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direction: direction/forward
top level: BBa_I14052
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orientation: inline

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type: ComponentDefinition
persistent identity: BBa I13305
display id: BBa_I13305
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: R0011.I13279 Lac-Regulated Las Amplifier
creator: Debra and Jay and Barry
created: 2004-08-08T11:00:00Z
modified: 2015-08-31T04:07:33Z
discontinued: false
dominant: false
experience: experience/None
group u list: _6_
m user id: 0
owner id: 101
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_I13279
component: BBa_R0011
sequence annotation: BBa_I13279
sequence annotation: BBa_R0011
sequence: https://synbiohub.org/public/igem/BBa_I13305_sequence/1

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persistent identity: component2223575
display id: component2223575
version: 1
top level: BBa_I13305
access: public
definition: BBa_I13279

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persistent identity: component2223556
display id: component2223556
version: 1
top level: BBa_I13305
access: public
definition: https://synbiohub.org/public/igem/BBa_R0011/1

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persistent identity: range2223575
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orientation: inline

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display id: annotation2223556
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persistent identity: range2223556
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type: ComponentDefinition
persistent identity: BBa I12056
display id: BBa_I12056
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: A protein (lambda cI) subassembly for modified BL oscillator based on I12036
creator: endelman
created: 2004-09-02T11:00:00Z
modified: 2015-08-31T04:07:31Z
discontinued: false
dominant: false
experience: experience/None
group u list: _3_
m user id: 0
owner id: 145
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This part produces POPS output to connect with a reporter.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_C0051
component: BBa_I12036
component: BBa_B0012
component: BBa_B0010
component: BBa_B0034
sequence annotation: BBa_B0012
sequence annotation: BBa_B0010
sequence annotation: BBa_I12036
sequence annotation: BBa_B0034
sequence annotation: BBa_C0051
sequence: https://synbiohub.org/public/igem/BBa_I12056_sequence/1

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version: 1
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definition: https://synbiohub.org/public/igem/BBa_C0051/1

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definition: https://synbiohub.org/public/igem/BBa_I12036/1

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display id: component1241631
version: 1
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access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

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definition: https://synbiohub.org/public/igem/BBa_B0034/1

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display id: annotation1241631
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persistent identity: range1241605
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component: BBa_C0051

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type: Range
persistent identity: range1241615
display id: range1241615
version: 1
direction: direction/forward
top level: BBa_I12056
start: 118
end: 867
orientation: inline

BBa_I12036
type: SequenceAnnotation
persistent identity: annotation1241657
display id: annotation1241657
version: 1
top level: BBa_I12056
location: https://synbiohub.org/public/igem/BBa_I12056/annotation1241657/range1241657/1
component: BBa_I12036

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type: Range
persistent identity: range1241657
display id: range1241657
version: 1
direction: direction/forward
top level: BBa_I12056
start: 1038
end: 1128
orientation: inline

BBa_S04003
type: ComponentDefinition
persistent identity: BBa S04003
display id: BBa_S04003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Pbad . TetR.
creator: CC Signalling
created: 2004-07-18T11:00:00Z
modified: 2015-05-08T01:14:33Z
discontinued: false
dominant: false
experience: experience/None
group u list: _6_
m user id: 0
owner id: 101
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0010
component: BBa_B0012
component: BBa_I0500
component: BBa_C0040
component: BBa_B0034
sequence annotation: BBa_B0010
sequence annotation: BBa_B0034
sequence annotation: BBa_B0012
sequence annotation: BBa_I0500
sequence annotation: BBa_C0040
sequence: https://synbiohub.org/public/igem/BBa_S04003_sequence/1

BBa_B0010
type: Component
persistent identity: component955545
display id: component955545
version: 1
top level: BBa_S04003
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0012
type: Component
persistent identity: component955555
display id: component955555
version: 1
top level: BBa_S04003
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_I0500
type: Component
persistent identity: component955524
display id: component955524
version: 1
top level: BBa_S04003
access: public
definition: https://synbiohub.org/public/igem/BBa_I0500/1

BBa_C0040
type: Component
persistent identity: component955539
display id: component955539
version: 1
top level: BBa_S04003
access: public
definition: https://synbiohub.org/public/igem/BBa_C0040/1

BBa_B0034
type: Component
persistent identity: component955529
display id: component955529
version: 1
top level: BBa_S04003
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation955545
display id: annotation955545
version: 1
top level: BBa_S04003
location: https://synbiohub.org/public/igem/BBa_S04003/annotation955545/range955545/1
component: BBa_B0010

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type: Range
persistent identity: range955545
display id: range955545
version: 1
direction: direction/forward
top level: BBa_S04003
start: 1930
end: 2009
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation955529
display id: annotation955529
version: 1
top level: BBa_S04003
location: https://synbiohub.org/public/igem/BBa_S04003/annotation955529/range955529/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_S04003/annotation955529/range955529/1
type: Range
persistent identity: range955529
display id: range955529
version: 1
direction: direction/forward
top level: BBa_S04003
start: 1219
end: 1230
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation955555
display id: annotation955555
version: 1
top level: BBa_S04003
location: https://synbiohub.org/public/igem/BBa_S04003/annotation955555/range955555/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_S04003/annotation955555/range955555/1
type: Range
persistent identity: range955555
display id: range955555
version: 1
direction: direction/forward
top level: BBa_S04003
start: 2018
end: 2058
orientation: inline

BBa_I0500
type: SequenceAnnotation
persistent identity: annotation955524
display id: annotation955524
version: 1
top level: BBa_S04003
location: https://synbiohub.org/public/igem/BBa_S04003/annotation955524/range955524/1
component: BBa_I0500

https://synbiohub.org/public/igem/BBa_S04003/annotation955524/range955524/1
type: Range
persistent identity: range955524
display id: range955524
version: 1
direction: direction/forward
top level: BBa_S04003
start: 1
end: 1210
orientation: inline

BBa_C0040
type: SequenceAnnotation
persistent identity: annotation955539
display id: annotation955539
version: 1
top level: BBa_S04003
location: https://synbiohub.org/public/igem/BBa_S04003/annotation955539/range955539/1
component: BBa_C0040

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type: Range
persistent identity: range955539
display id: range955539
version: 1
direction: direction/forward
top level: BBa_S04003
start: 1237
end: 1896
orientation: inline

lacI
type: ComponentDefinition
persistent identity: BBa Q03121
display id: BBa_Q03121
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: QPI (LacI)
creator: Caitlin Conboy and Jennifer Braff
created: 2003-12-02T12:00:00Z
modified: 2015-05-08T01:14:13Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000804
role: partType/Inverter
component: BBa_B0033
component: BBa_B0015
component: BBa_R0011
component: BBa_C0012
sequence annotation: BBa_C0012
sequence annotation: BBa_R0011
sequence annotation: BBa_B0033
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_Q03121_sequence/1

BBa_B0033
type: Component
persistent identity: component2221152
display id: component2221152
version: 1
top level: lacI
access: public
definition: https://synbiohub.org/public/igem/BBa_B0033/1

BBa_B0015
type: Component
persistent identity: component2221164
display id: component2221164
version: 1
top level: lacI
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_R0011
type: Component
persistent identity: component2221165
display id: component2221165
version: 1
top level: lacI
access: public
definition: https://synbiohub.org/public/igem/BBa_R0011/1

BBa_C0012
type: Component
persistent identity: component2221155
display id: component2221155
version: 1
top level: lacI
access: public
definition: https://synbiohub.org/public/igem/BBa_C0012/1

BBa_C0012
type: SequenceAnnotation
persistent identity: annotation2221155
display id: annotation2221155
version: 1
top level: lacI
location: https://synbiohub.org/public/igem/BBa_Q03121/annotation2221155/range2221155/1
component: BBa_C0012

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type: Range
persistent identity: range2221155
display id: range2221155
version: 1
direction: direction/forward
top level: lacI
start: 18
end: 1145
orientation: inline

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation2221165
display id: annotation2221165
version: 1
top level: lacI
location: https://synbiohub.org/public/igem/BBa_Q03121/annotation2221165/range2221165/1
component: BBa_R0011

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type: Range
persistent identity: range2221165
display id: range2221165
version: 1
direction: direction/forward
top level: lacI
start: 1316
end: 1369
orientation: inline

BBa_B0033
type: SequenceAnnotation
persistent identity: annotation2221152
display id: annotation2221152
version: 1
top level: lacI
location: https://synbiohub.org/public/igem/BBa_Q03121/annotation2221152/range2221152/1
component: BBa_B0033

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type: Range
persistent identity: range2221152
display id: range2221152
version: 1
direction: direction/forward
top level: lacI
start: 1
end: 11
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2221164
display id: annotation2221164
version: 1
top level: lacI
location: https://synbiohub.org/public/igem/BBa_Q03121/annotation2221164/range2221164/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_Q03121/annotation2221164/range2221164/1
type: Range
persistent identity: range2221164
display id: range2221164
version: 1
direction: direction/reverse
top level: lacI
start: 1179
end: 1307
orientation: inline

BBa_I0409
type: ComponentDefinition
persistent identity: BBa I0409
display id: BBa_I0409
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: R0040.B0034.C0076.E0420
creator: jcbraff
created: 2004-06-08T11:00:00Z
modified: 2015-08-31T04:07:28Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_1_
m user id: 0
owner id: 61
owning group id: 7
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: R0040.B0034.C0076.E0420
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_E0020
component: BBa_B0010
component: BBa_B0034
component: BBa_C0076
component: BBa_B0012
component: BBa_R0040
sequence annotation: BBa_C0076
sequence annotation: BBa_B0034
sequence annotation: BBa_E0020
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_R0040
sequence: https://synbiohub.org/public/igem/BBa_I0409_sequence/1

BBa_E0020
type: Component
persistent identity: component958851
display id: component958851
version: 1
top level: BBa_I0409
access: public
definition: https://synbiohub.org/public/igem/BBa_E0020/1

BBa_B0010
type: Component
persistent identity: component958856
display id: component958856
version: 1
top level: BBa_I0409
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0034
type: Component
persistent identity: component958849
display id: component958849
version: 1
top level: BBa_I0409
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0034
type: Component
persistent identity: component958833
display id: component958833
version: 1
top level: BBa_I0409
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_C0076
type: Component
persistent identity: component958843
display id: component958843
version: 1
top level: BBa_I0409
access: public
definition: https://synbiohub.org/public/igem/BBa_C0076/1

BBa_B0012
type: Component
persistent identity: component958866
display id: component958866
version: 1
top level: BBa_I0409
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_R0040
type: Component
persistent identity: component958825
display id: component958825
version: 1
top level: BBa_I0409
access: public
definition: https://synbiohub.org/public/igem/BBa_R0040/1

BBa_C0076
type: SequenceAnnotation
persistent identity: annotation958843
display id: annotation958843
version: 1
top level: BBa_I0409
location: https://synbiohub.org/public/igem/BBa_I0409/annotation958843/range958843/1
component: BBa_C0076

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type: Range
persistent identity: range958843
display id: range958843
version: 1
direction: direction/forward
top level: BBa_I0409
start: 81
end: 782
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation958849
display id: annotation958849
version: 1
top level: BBa_I0409
location: https://synbiohub.org/public/igem/BBa_I0409/annotation958849/range958849/1
component: BBa_B0034

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type: Range
persistent identity: range958849
display id: range958849
version: 1
direction: direction/forward
top level: BBa_I0409
start: 816
end: 827
orientation: inline

BBa_E0020
type: SequenceAnnotation
persistent identity: annotation958851
display id: annotation958851
version: 1
top level: BBa_I0409
location: https://synbiohub.org/public/igem/BBa_I0409/annotation958851/range958851/1
component: BBa_E0020

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type: Range
persistent identity: range958851
display id: range958851
version: 1
direction: direction/forward
top level: BBa_I0409
start: 834
end: 1556
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation958833
display id: annotation958833
version: 1
top level: BBa_I0409
location: https://synbiohub.org/public/igem/BBa_I0409/annotation958833/range958833/1
component: BBa_B0034

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type: Range
persistent identity: range958833
display id: range958833
version: 1
direction: direction/forward
top level: BBa_I0409
start: 63
end: 74
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation958856
display id: annotation958856
version: 1
top level: BBa_I0409
location: https://synbiohub.org/public/igem/BBa_I0409/annotation958856/range958856/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I0409/annotation958856/range958856/1
type: Range
persistent identity: range958856
display id: range958856
version: 1
direction: direction/forward
top level: BBa_I0409
start: 1565
end: 1644
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation958866
display id: annotation958866
version: 1
top level: BBa_I0409
location: https://synbiohub.org/public/igem/BBa_I0409/annotation958866/range958866/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I0409/annotation958866/range958866/1
type: Range
persistent identity: range958866
display id: range958866
version: 1
direction: direction/forward
top level: BBa_I0409
start: 1653
end: 1693
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation958825
display id: annotation958825
version: 1
top level: BBa_I0409
location: https://synbiohub.org/public/igem/BBa_I0409/annotation958825/range958825/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_I0409/annotation958825/range958825/1
type: Range
persistent identity: range958825
display id: range958825
version: 1
direction: direction/forward
top level: BBa_I0409
start: 1
end: 54
orientation: inline

BBa_I5311
type: ComponentDefinition
persistent identity: BBa I5311
display id: BBa_I5311
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Plac. Regulatable YFP LVA-
creator: Reshma Shetty
created: 2005-06-29T11:00:00Z
modified: 2015-08-31T04:07:42Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _41_
m user id: 0
owner id: 126
owning group id: 6
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_E0030
component: BBa_B0034
component: BBa_R0011
component: BBa_B0011
component: BBa_B0012
sequence annotation: BBa_B0034
sequence annotation: BBa_R0011
sequence annotation: BBa_E0030
sequence annotation: BBa_B0011
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_I5311_sequence/1

BBa_E0030
type: Component
persistent identity: component1557885
display id: component1557885
version: 1
top level: BBa_I5311
access: public
definition: https://synbiohub.org/public/igem/BBa_E0030/1

BBa_B0034
type: Component
persistent identity: component1557883
display id: component1557883
version: 1
top level: BBa_I5311
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_R0011
type: Component
persistent identity: component1557874
display id: component1557874
version: 1
top level: BBa_I5311
access: public
definition: https://synbiohub.org/public/igem/BBa_R0011/1

BBa_B0011
type: Component
persistent identity: component1557902
display id: component1557902
version: 1
top level: BBa_I5311
access: public
definition: https://synbiohub.org/public/igem/BBa_B0011/1

BBa_B0012
type: Component
persistent identity: component1557894
display id: component1557894
version: 1
top level: BBa_I5311
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1557883
display id: annotation1557883
version: 1
top level: BBa_I5311
location: https://synbiohub.org/public/igem/BBa_I5311/annotation1557883/range1557883/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I5311/annotation1557883/range1557883/1
type: Range
persistent identity: range1557883
display id: range1557883
version: 1
direction: direction/forward
top level: BBa_I5311
start: 64
end: 75
orientation: inline

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation1557874
display id: annotation1557874
version: 1
top level: BBa_I5311
location: https://synbiohub.org/public/igem/BBa_I5311/annotation1557874/range1557874/1
component: BBa_R0011

https://synbiohub.org/public/igem/BBa_I5311/annotation1557874/range1557874/1
type: Range
persistent identity: range1557874
display id: range1557874
version: 1
direction: direction/forward
top level: BBa_I5311
start: 1
end: 54
orientation: inline

BBa_E0030
type: SequenceAnnotation
persistent identity: annotation1557885
display id: annotation1557885
version: 1
top level: BBa_I5311
location: https://synbiohub.org/public/igem/BBa_I5311/annotation1557885/range1557885/1
component: BBa_E0030

https://synbiohub.org/public/igem/BBa_I5311/annotation1557885/range1557885/1
type: Range
persistent identity: range1557885
display id: range1557885
version: 1
direction: direction/forward
top level: BBa_I5311
start: 82
end: 804
orientation: inline

BBa_B0011
type: SequenceAnnotation
persistent identity: annotation1557902
display id: annotation1557902
version: 1
top level: BBa_I5311
location: https://synbiohub.org/public/igem/BBa_I5311/annotation1557902/range1557902/1
component: BBa_B0011

https://synbiohub.org/public/igem/BBa_I5311/annotation1557902/range1557902/1
type: Range
persistent identity: range1557902
display id: range1557902
version: 1
direction: direction/forward
top level: BBa_I5311
start: 862
end: 907
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1557894
display id: annotation1557894
version: 1
top level: BBa_I5311
location: https://synbiohub.org/public/igem/BBa_I5311/annotation1557894/range1557894/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I5311/annotation1557894/range1557894/1
type: Range
persistent identity: range1557894
display id: range1557894
version: 1
direction: direction/forward
top level: BBa_I5311
start: 813
end: 853
orientation: inline

BBa_R4037
type: ComponentDefinition
persistent identity: BBa R4037
display id: BBa_R4037
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Tet repressible promoter and dedicated RBS
creator: Barry Canton
created: 2005-12-06T12:00:00Z
modified: 2015-05-08T01:14:16Z
discontinued: false
dominant: false
group u list: _11_6_
m user id: 0
owner id: 135
owning group id: 6
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This composite part is composed of a tet repressible promoter and a dedicated RBS. It will be used to construct a reporter system for VM1.0. GFP production from the reporter system can be compared to the GFP production of a similar system that uses a T7 promoter instead of an E. coli promoter and to I7102 which uses a slightly different dedicated RBS.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0037
component: BBa_R0040
sequence annotation: BBa_R0040
sequence annotation: BBa_B0037
sequence: https://synbiohub.org/public/igem/BBa_R4037_sequence/1

BBa_B0037
type: Component
persistent identity: component1760610
display id: component1760610
version: 1
top level: BBa_R4037
access: public
definition: https://synbiohub.org/public/igem/BBa_B0037/1

BBa_R0040
type: Component
persistent identity: component1760605
display id: component1760605
version: 1
top level: BBa_R4037
access: public
definition: https://synbiohub.org/public/igem/BBa_R0040/1

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1760605
display id: annotation1760605
version: 1
top level: BBa_R4037
location: https://synbiohub.org/public/igem/BBa_R4037/annotation1760605/range1760605/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_R4037/annotation1760605/range1760605/1
type: Range
persistent identity: range1760605
display id: range1760605
version: 1
direction: direction/forward
top level: BBa_R4037
start: 1
end: 54
orientation: inline

BBa_B0037
type: SequenceAnnotation
persistent identity: annotation1760610
display id: annotation1760610
version: 1
top level: BBa_R4037
location: https://synbiohub.org/public/igem/BBa_R4037/annotation1760610/range1760610/1
component: BBa_B0037

https://synbiohub.org/public/igem/BBa_R4037/annotation1760610/range1760610/1
type: Range
persistent identity: range1760610
display id: range1760610
version: 1
direction: direction/forward
top level: BBa_R4037
start: 63
end: 72
orientation: inline

BBa_I14002
type: ComponentDefinition
persistent identity: BBa I14002
display id: BBa_I14002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LacI with strong RBS
creator: Aditi Shrivastava, Jiwon Lee, Sarah Welch
created: 2004-07-25T11:00:00Z
modified: 2015-08-31T04:07:37Z
discontinued: false
dominant: false
experience: experience/None
group u list: _4_
m user id: 0
owner id: 166
owning group id: 7
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: BBa_B0030 BBa_C0012
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0030
component: BBa_C0012
sequence annotation: BBa_C0012
sequence annotation: BBa_B0030
sequence: https://synbiohub.org/public/igem/BBa_I14002_sequence/1

BBa_B0030
type: Component
persistent identity: component939513
display id: component939513
version: 1
top level: BBa_I14002
access: public
definition: https://synbiohub.org/public/igem/BBa_B0030/1

BBa_C0012
type: Component
persistent identity: component939524
display id: component939524
version: 1
top level: BBa_I14002
access: public
definition: https://synbiohub.org/public/igem/BBa_C0012/1

BBa_C0012
type: SequenceAnnotation
persistent identity: annotation939524
display id: annotation939524
version: 1
top level: BBa_I14002
location: https://synbiohub.org/public/igem/BBa_I14002/annotation939524/range939524/1
component: BBa_C0012

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persistent identity: range939524
display id: range939524
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direction: direction/forward
top level: BBa_I14002
start: 22
end: 1149
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation939513
display id: annotation939513
version: 1
top level: BBa_I14002
location: https://synbiohub.org/public/igem/BBa_I14002/annotation939513/range939513/1
component: BBa_B0030

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type: Range
persistent identity: range939513
display id: range939513
version: 1
direction: direction/forward
top level: BBa_I14002
start: 1
end: 15
orientation: inline

BBa_Q01530
type: ComponentDefinition
persistent identity: BBa Q01530
display id: BBa_Q01530
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: QPI (B0031.C0053.B0015.R0053)
creator: Caitlin Conboy and Jennifer Braff
created: 2003-12-02T12:00:00Z
modified: 2015-05-08T01:14:13Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_R0053
component: BBa_B0010
component: BBa_B0012
component: BBa_B0031
component: BBa_C0053
sequence annotation: BBa_R0053
sequence annotation: BBa_B0031
sequence annotation: BBa_B0010
sequence annotation: BBa_C0053
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_Q01530_sequence/1

BBa_R0053
type: Component
persistent identity: component952075
display id: component952075
version: 1
top level: BBa_Q01530
access: public
definition: https://synbiohub.org/public/igem/BBa_R0053/1

BBa_B0010
type: Component
persistent identity: component952046
display id: component952046
version: 1
top level: BBa_Q01530
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0012
type: Component
persistent identity: component952056
display id: component952056
version: 1
top level: BBa_Q01530
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0031
type: Component
persistent identity: component952026
display id: component952026
version: 1
top level: BBa_Q01530
access: public
definition: https://synbiohub.org/public/igem/BBa_B0031/1

BBa_C0053
type: Component
persistent identity: component952038
display id: component952038
version: 1
top level: BBa_Q01530
access: public
definition: https://synbiohub.org/public/igem/BBa_C0053/1

BBa_R0053
type: SequenceAnnotation
persistent identity: annotation952075
display id: annotation952075
version: 1
top level: BBa_Q01530
location: https://synbiohub.org/public/igem/BBa_Q01530/annotation952075/range952075/1
component: BBa_R0053

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display id: range952075
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top level: BBa_Q01530
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end: 931
orientation: inline

BBa_B0031
type: SequenceAnnotation
persistent identity: annotation952026
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version: 1
top level: BBa_Q01530
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component: BBa_B0031

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type: Range
persistent identity: range952026
display id: range952026
version: 1
direction: direction/forward
top level: BBa_Q01530
start: 1
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orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation952046
display id: annotation952046
version: 1
top level: BBa_Q01530
location: https://synbiohub.org/public/igem/BBa_Q01530/annotation952046/range952046/1
component: BBa_B0010

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type: Range
persistent identity: range952046
display id: range952046
version: 1
direction: direction/forward
top level: BBa_Q01530
start: 741
end: 820
orientation: inline

BBa_C0053
type: SequenceAnnotation
persistent identity: annotation952038
display id: annotation952038
version: 1
top level: BBa_Q01530
location: https://synbiohub.org/public/igem/BBa_Q01530/annotation952038/range952038/1
component: BBa_C0053

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type: Range
persistent identity: range952038
display id: range952038
version: 1
direction: direction/forward
top level: BBa_Q01530
start: 21
end: 707
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation952056
display id: annotation952056
version: 1
top level: BBa_Q01530
location: https://synbiohub.org/public/igem/BBa_Q01530/annotation952056/range952056/1
component: BBa_B0012

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type: Range
persistent identity: range952056
display id: range952056
version: 1
direction: direction/forward
top level: BBa_Q01530
start: 829
end: 869
orientation: inline

BBa_Q03520
type: ComponentDefinition
persistent identity: BBa Q03520
display id: BBa_Q03520
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: QPI (B0033.C0052.B0015.R0052)
creator: Caitlin Conboy and Jennifer Braff
created: 2003-12-02T12:00:00Z
modified: 2015-05-08T01:14:13Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_C0052
component: BBa_B0033
component: BBa_B0010
component: BBa_R0052
component: BBa_B0012
sequence annotation: BBa_B0010
sequence annotation: BBa_C0052
sequence annotation: BBa_R0052
sequence annotation: BBa_B0012
sequence annotation: BBa_B0033
sequence: https://synbiohub.org/public/igem/BBa_Q03520_sequence/1

BBa_C0052
type: Component
persistent identity: component952531
display id: component952531
version: 1
top level: BBa_Q03520
access: public
definition: https://synbiohub.org/public/igem/BBa_C0052/1

BBa_B0033
type: Component
persistent identity: component952520
display id: component952520
version: 1
top level: BBa_Q03520
access: public
definition: https://synbiohub.org/public/igem/BBa_B0033/1

BBa_B0010
type: Component
persistent identity: component952538
display id: component952538
version: 1
top level: BBa_Q03520
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_R0052
type: Component
persistent identity: component952570
display id: component952570
version: 1
top level: BBa_Q03520
access: public
definition: https://synbiohub.org/public/igem/BBa_R0052/1

BBa_B0012
type: Component
persistent identity: component952548
display id: component952548
version: 1
top level: BBa_Q03520
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation952538
display id: annotation952538
version: 1
top level: BBa_Q03520
location: https://synbiohub.org/public/igem/BBa_Q03520/annotation952538/range952538/1
component: BBa_B0010

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type: Range
persistent identity: range952538
display id: range952538
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direction: direction/forward
top level: BBa_Q03520
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end: 799
orientation: inline

BBa_C0052
type: SequenceAnnotation
persistent identity: annotation952531
display id: annotation952531
version: 1
top level: BBa_Q03520
location: https://synbiohub.org/public/igem/BBa_Q03520/annotation952531/range952531/1
component: BBa_C0052

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type: Range
persistent identity: range952531
display id: range952531
version: 1
direction: direction/forward
top level: BBa_Q03520
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end: 686
orientation: inline

BBa_R0052
type: SequenceAnnotation
persistent identity: annotation952570
display id: annotation952570
version: 1
top level: BBa_Q03520
location: https://synbiohub.org/public/igem/BBa_Q03520/annotation952570/range952570/1
component: BBa_R0052

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type: Range
persistent identity: range952570
display id: range952570
version: 1
direction: direction/forward
top level: BBa_Q03520
start: 857
end: 902
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation952548
display id: annotation952548
version: 1
top level: BBa_Q03520
location: https://synbiohub.org/public/igem/BBa_Q03520/annotation952548/range952548/1
component: BBa_B0012

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type: Range
persistent identity: range952548
display id: range952548
version: 1
direction: direction/forward
top level: BBa_Q03520
start: 808
end: 848
orientation: inline

BBa_B0033
type: SequenceAnnotation
persistent identity: annotation952520
display id: annotation952520
version: 1
top level: BBa_Q03520
location: https://synbiohub.org/public/igem/BBa_Q03520/annotation952520/range952520/1
component: BBa_B0033

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type: Range
persistent identity: range952520
display id: range952520
version: 1
direction: direction/forward
top level: BBa_Q03520
start: 1
end: 11
orientation: inline

BBa_I14042
type: ComponentDefinition
persistent identity: BBa I14042
display id: BBa_I14042
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: strong RBS . penI
creator: Aditi Shrivastava, Sarah Welch, Jiwon Lee
created: 2004-08-03T11:00:00Z
modified: 2015-08-31T04:07:38Z
discontinued: false
dominant: false
experience: experience/None
group u list: _4_
m user id: 0
owner id: 166
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: BBa_B0030 BBa_C0074
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_C0074
component: BBa_B0030
sequence annotation: BBa_C0074
sequence annotation: BBa_B0030
sequence: https://synbiohub.org/public/igem/BBa_I14042_sequence/1

BBa_C0074
type: Component
persistent identity: component1023943
display id: component1023943
version: 1
top level: BBa_I14042
access: public
definition: https://synbiohub.org/public/igem/BBa_C0074/1

BBa_B0030
type: Component
persistent identity: component1023932
display id: component1023932
version: 1
top level: BBa_I14042
access: public
definition: https://synbiohub.org/public/igem/BBa_B0030/1

BBa_C0074
type: SequenceAnnotation
persistent identity: annotation1023943
display id: annotation1023943
version: 1
top level: BBa_I14042
location: https://synbiohub.org/public/igem/BBa_I14042/annotation1023943/range1023943/1
component: BBa_C0074

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type: Range
persistent identity: range1023943
display id: range1023943
version: 1
direction: direction/forward
top level: BBa_I14042
start: 22
end: 444
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation1023932
display id: annotation1023932
version: 1
top level: BBa_I14042
location: https://synbiohub.org/public/igem/BBa_I14042/annotation1023932/range1023932/1
component: BBa_B0030

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type: Range
persistent identity: range1023932
display id: range1023932
version: 1
direction: direction/forward
top level: BBa_I14042
start: 1
end: 15
orientation: inline

tetR
type: ComponentDefinition
persistent identity: BBa Q03400
display id: BBa_Q03400
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: QPI (tetR)
creator: Caitlin Conboy and Jennifer Braff
created: 2003-12-02T12:00:00Z
modified: 2015-05-08T01:14:13Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000804
role: partType/Inverter
component: BBa_B0012
component: BBa_R0040
component: BBa_B0010
component: BBa_C0040
component: BBa_B0033
sequence annotation: BBa_C0040
sequence annotation: BBa_B0010
sequence annotation: BBa_B0033
sequence annotation: BBa_R0040
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_Q03400_sequence/1

BBa_B0012
type: Component
persistent identity: component952318
display id: component952318
version: 1
top level: tetR
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_R0040
type: Component
persistent identity: component952333
display id: component952333
version: 1
top level: tetR
access: public
definition: https://synbiohub.org/public/igem/BBa_R0040/1

BBa_B0010
type: Component
persistent identity: component952308
display id: component952308
version: 1
top level: tetR
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_C0040
type: Component
persistent identity: component952302
display id: component952302
version: 1
top level: tetR
access: public
definition: https://synbiohub.org/public/igem/BBa_C0040/1

BBa_B0033
type: Component
persistent identity: component952291
display id: component952291
version: 1
top level: tetR
access: public
definition: https://synbiohub.org/public/igem/BBa_B0033/1

BBa_C0040
type: SequenceAnnotation
persistent identity: annotation952302
display id: annotation952302
version: 1
top level: tetR
location: https://synbiohub.org/public/igem/BBa_Q03400/annotation952302/range952302/1
component: BBa_C0040

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type: Range
persistent identity: range952302
display id: range952302
version: 1
direction: direction/forward
top level: tetR
start: 18
end: 677
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation952308
display id: annotation952308
version: 1
top level: tetR
location: https://synbiohub.org/public/igem/BBa_Q03400/annotation952308/range952308/1
component: BBa_B0010

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type: Range
persistent identity: range952308
display id: range952308
version: 1
direction: direction/forward
top level: tetR
start: 711
end: 790
orientation: inline

BBa_B0033
type: SequenceAnnotation
persistent identity: annotation952291
display id: annotation952291
version: 1
top level: tetR
location: https://synbiohub.org/public/igem/BBa_Q03400/annotation952291/range952291/1
component: BBa_B0033

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type: Range
persistent identity: range952291
display id: range952291
version: 1
direction: direction/forward
top level: tetR
start: 1
end: 11
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation952333
display id: annotation952333
version: 1
top level: tetR
location: https://synbiohub.org/public/igem/BBa_Q03400/annotation952333/range952333/1
component: BBa_R0040

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type: Range
persistent identity: range952333
display id: range952333
version: 1
direction: direction/forward
top level: tetR
start: 848
end: 901
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation952318
display id: annotation952318
version: 1
top level: tetR
location: https://synbiohub.org/public/igem/BBa_Q03400/annotation952318/range952318/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_Q03400/annotation952318/range952318/1
type: Range
persistent identity: range952318
display id: range952318
version: 1
direction: direction/forward
top level: tetR
start: 799
end: 839
orientation: inline

BBa_I13500
type: ComponentDefinition
persistent identity: BBa I13500
display id: BBa_I13500
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Screening plasmid intermediate
creator: jkm
created: 2005-05-30T11:00:00Z
modified: 2015-08-31T04:07:34Z
discontinued: false
dominant: true
experience: experience/None
group u list: _11_
m user id: 0
owner id: 253
owning group id: 6
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Built by Josh as an intermediate in screening plasmid construction
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0034
component: BBa_E0040
sequence annotation: BBa_E0040
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_I13500_sequence/1

BBa_B0034
type: Component
persistent identity: component1505000
display id: component1505000
version: 1
top level: BBa_I13500
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_E0040
type: Component
persistent identity: component1505002
display id: component1505002
version: 1
top level: BBa_I13500
access: public
definition: https://synbiohub.org/public/igem/BBa_E0040/1

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation1505002
display id: annotation1505002
version: 1
top level: BBa_I13500
location: https://synbiohub.org/public/igem/BBa_I13500/annotation1505002/range1505002/1
component: BBa_E0040

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type: Range
persistent identity: range1505002
display id: range1505002
version: 1
direction: direction/forward
top level: BBa_I13500
start: 19
end: 738
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1505000
display id: annotation1505000
version: 1
top level: BBa_I13500
location: https://synbiohub.org/public/igem/BBa_I13500/annotation1505000/range1505000/1
component: BBa_B0034

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type: Range
persistent identity: range1505000
display id: range1505000
version: 1
direction: direction/forward
top level: BBa_I13500
start: 1
end: 12
orientation: inline

BBa_J06916
type: ComponentDefinition
persistent identity: BBa J06916
display id: BBa_J06916
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Lambda cI QPI regulated by LacI with EYFP
creator: Yves Wang
created: 2005-07-31T11:00:00Z
modified: 2015-08-31T04:08:19Z
discontinued: false
dominant: false
experience: experience/Fails
group u list: _20_
m user id: 0
owner id: 340
owning group id: 20
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: -- No description --
mutable notes: Does not glow even in Lac+ cells.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0012
component: BBa_E0030
component: BBa_R0011
component: BBa_B0010
component: BBa_B0034
component: BBa_C0051
component: BBa_R0051
sequence annotation: BBa_R0011
sequence annotation: BBa_E0030
sequence annotation: BBa_R0051
sequence annotation: BBa_B0034
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_C0051
sequence: https://synbiohub.org/public/igem/BBa_J06916_sequence/1

BBa_B0012
type: Component
persistent identity: component1599711
display id: component1599711
version: 1
top level: BBa_J06916
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_E0030
type: Component
persistent identity: component1599736
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description: Tet Toggle (medium RBS, weaker RBS)
creator: Vikram Vijayan, Allen Hsu, Lawrence Fomundam
created: 2004-08-03T11:00:00Z
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discontinued: false
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experience: experience/None
group u list: _4_
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bookmark: false
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was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: I13307.E0430 Lac-Regulated Cin Amplifier with YFP Reporter
creator: Debra and Jay and Barry
created: 2004-08-08T11:00:00Z
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discontinued: false
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experience: experience/None
group u list: _6_
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sample status: Not in stock
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bookmark: false
mutable description: -- No description --
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was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: C12, C12 Router (medium RBS)
creator: Vikram Vijayan, Allen Hsu, Lawrence Fomundam
created: 2004-08-03T11:00:00Z
modified: 2015-08-31T04:07:38Z
discontinued: false
dominant: false
experience: experience/None
group u list: _4_
m user id: 0
owner id: 171
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: p(Las) CI O, Medium RBS, LasI + LVA
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owned by: myers
star: false
type: DnaRegion
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component: BBa_I14016
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was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: B0034.C0076.B0015
creator: cconboy
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modified: 2015-05-08T01:14:11Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_1_
m user id: 0
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sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: -- No description --
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star: false
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BBa_J07035
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version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: FecA'-GFP under inducible promoter and rbs
creator: jenmitch
created: 2005-07-19T11:00:00Z
modified: 2015-08-31T04:08:19Z
discontinued: false
dominant: false
experience: experience/None
group u list: _21_
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bookmark: false
mutable description: -- No description --
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star: false
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BBa_I12054
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display id: BBa_I12054
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: B protein (434 cI) subassembly for modified BL oscillator
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modified: 2015-08-31T04:07:31Z
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dominant: false
experience: experience/None
group u list: _3_
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bookmark: false
mutable description: This part produces 434 cI (B) in presence of lambda cI (A).
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owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
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component: BBa_B0012
component: BBa_B0010
component: BBa_I12007
component: BBa_B0034
sequence annotation: BBa_B0012
sequence annotation: BBa_B0034
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BBa_C0056
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access: public
definition: https://synbiohub.org/public/igem/BBa_C0056/1

BBa_B0012
type: Component
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BBa_B0010
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access: public
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BBa_I12007
type: Component
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access: public
definition: https://synbiohub.org/public/igem/BBa_I12007/1

BBa_B0034
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BBa_B0012
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type: SequenceAnnotation
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BBa_B0010
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BBa_C0056
type: SequenceAnnotation
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start: 109
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BBa_I12007
type: SequenceAnnotation
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version: 1
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location: https://synbiohub.org/public/igem/BBa_I12054/annotation1241967/range1241967/1
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start: 1
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BBa_I5320
type: ComponentDefinition
persistent identity: BBa I5320
display id: BBa_I5320
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Olmecs: Reporter for Repressilator versions
creator: Olmecs 2003 IAP Team
created: 2003-11-27T12:00:00Z
modified: 2015-08-31T04:07:42Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: For reference only
status: status/Informational
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_R0040
component: BBa_E0032
component: BBa_B0012
component: BBa_B0034
component: BBa_B0011
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sequence annotation: BBa_E0032
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sequence: https://synbiohub.org/public/igem/BBa_I5320_sequence/1

BBa_R0040
type: Component
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version: 1
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access: public
definition: https://synbiohub.org/public/igem/BBa_R0040/1

BBa_E0032
type: Component
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version: 1
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access: public
definition: https://synbiohub.org/public/igem/BBa_E0032/1

BBa_B0012
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definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0034
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version: 1
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definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0011
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type: SequenceAnnotation
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type: SequenceAnnotation
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version: 1
direction: direction/forward
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start: 1
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BBa_B0011
type: SequenceAnnotation
persistent identity: annotation948267
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version: 1
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location: https://synbiohub.org/public/igem/BBa_I5320/annotation948267/range948267/1
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version: 1
direction: direction/forward
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start: 900
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orientation: inline

BBa_I5510
type: ComponentDefinition
persistent identity: BBa I5510
display id: BBa_I5510
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Olmecs: Synchro-Dualator part 2
creator: Olmecs 2003 IAP Team
created: 2003-11-27T12:00:00Z
modified: 2015-08-31T04:07:42Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: For reference only
status: status/Informational
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_I5410
component: BBa_I5310
sequence annotation: BBa_I5410
sequence annotation: BBa_I5310
sequence: https://synbiohub.org/public/igem/BBa_I5510_sequence/1

BBa_I5410
type: Component
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display id: component2237351
version: 1
top level: BBa_I5510
access: public
definition: BBa_I5410

BBa_I5310
type: Component
persistent identity: component2237370
display id: component2237370
version: 1
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access: public
definition: BBa_I5310

BBa_I5410
type: SequenceAnnotation
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version: 1
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location: https://synbiohub.org/public/igem/BBa_I5510/annotation2237351/range2237351/1
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version: 1
direction: direction/reverse
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BBa_I5310
type: SequenceAnnotation
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location: https://synbiohub.org/public/igem/BBa_I5510/annotation2237370/range2237370/1
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type: Range
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version: 1
direction: direction/reverse
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start: 3126
end: 4071
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BBa_I13413
type: ComponentDefinition
persistent identity: BBa I13413
display id: BBa_I13413
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Pbad.RBS.CFP (insertion site) RBS.YFP
creator: jkm
created: 2005-05-22T11:00:00Z
modified: 2015-08-31T04:07:34Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_
m user id: 0
owner id: 253
owning group id: 6
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Intermediate in building second version of screening plasmid. Pbad-RBS-CFP-HindIII-NcoI-RBS-YFP
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_E0030
component: BBa_I13411
component: BBa_I0500
component: BBa_B0032
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sequence annotation: BBa_B0032
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sequence: https://synbiohub.org/public/igem/BBa_I13413_sequence/1

BBa_E0030
type: Component
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version: 1
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access: public
definition: https://synbiohub.org/public/igem/BBa_E0030/1

BBa_I13411
type: Component
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version: 1
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access: public
definition: https://synbiohub.org/public/igem/BBa_I13411/1

BBa_I0500
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access: public
definition: https://synbiohub.org/public/igem/BBa_I0500/1

BBa_B0032
type: Component
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display id: component1496216
version: 1
top level: BBa_I13413
access: public
definition: https://synbiohub.org/public/igem/BBa_B0032/1

BBa_E0030
type: SequenceAnnotation
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version: 1
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type: Range
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BBa_I0500
type: SequenceAnnotation
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BBa_B0032
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display id: range1496216
version: 1
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end: 1997
orientation: inline

BBa_I13411
type: SequenceAnnotation
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display id: annotation1496208
version: 1
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location: https://synbiohub.org/public/igem/BBa_I13413/annotation1496208/range1496208/1
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display id: range1496208
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start: 1219
end: 1976
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BBa_J05303
type: ComponentDefinition
persistent identity: BBa J05303
display id: BBa_J05303
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: R3 system A
creator: Alexander Roth
created: 2005-09-14T11:00:00Z
modified: 2015-08-31T04:08:15Z
discontinued: true
dominant: false
group u list: _34_
m user id: 0
owner id: 412
owning group id: 34
sample status: Discontinued
status: status/Deleted
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0010
component: BBa_B0034
component: BBa_B0012
component: BBa_J05101
component: BBa_J05213
sequence annotation: BBa_B0012
sequence annotation: BBa_J05213
sequence annotation: BBa_B0034
sequence annotation: BBa_J05101
sequence annotation: BBa_B0010
sequence: https://synbiohub.org/public/igem/BBa_J05303_sequence/1

BBa_B0010
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persistent identity: component1690286
display id: component1690286
version: 1
top level: BBa_J05303
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0034
type: Component
persistent identity: component1690276
display id: component1690276
version: 1
top level: BBa_J05303
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0012
type: Component
persistent identity: component1690296
display id: component1690296
version: 1
top level: BBa_J05303
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_J05101
type: Component
persistent identity: component1690280
display id: component1690280
version: 1
top level: BBa_J05303
access: public
definition: https://synbiohub.org/public/igem/BBa_J05101/1

BBa_J05213
type: Component
persistent identity: component1690271
display id: component1690271
version: 1
top level: BBa_J05303
access: public
definition: https://synbiohub.org/public/igem/BBa_J05213/1

BBa_B0012
type: SequenceAnnotation
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display id: annotation1690296
version: 1
top level: BBa_J05303
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type: Range
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display id: range1690296
version: 1
direction: direction/forward
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start: 481
end: 521
orientation: inline

BBa_J05213
type: SequenceAnnotation
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display id: annotation1690271
version: 1
top level: BBa_J05303
location: https://synbiohub.org/public/igem/BBa_J05303/annotation1690271/range1690271/1
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display id: range1690271
version: 1
direction: direction/forward
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orientation: inline

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direction: direction/forward
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orientation: inline

BBa_B0010
type: SequenceAnnotation
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display id: annotation1690286
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type: Range
persistent identity: range1690286
display id: range1690286
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direction: direction/forward
top level: BBa_J05303
start: 393
end: 472
orientation: inline

BBa_I14006
type: ComponentDefinition
persistent identity: BBa I14006
display id: BBa_I14006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CinR with strong RBS
creator: Aditi Shrivastava, Jiwon Lee, Sarah Welch
created: 2004-07-25T11:00:00Z
modified: 2015-08-31T04:07:37Z
discontinued: false
dominant: false
experience: experience/None
group u list: _4_
m user id: 0
owner id: 166
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: BBa_B0030 BBa_C0077
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0030
component: BBa_C0077
sequence annotation: BBa_C0077
sequence annotation: BBa_B0030
sequence: https://synbiohub.org/public/igem/BBa_I14006_sequence/1

BBa_B0030
type: Component
persistent identity: component1018225
display id: component1018225
version: 1
top level: BBa_I14006
access: public
definition: https://synbiohub.org/public/igem/BBa_B0030/1

BBa_C0077
type: Component
persistent identity: component1018236
display id: component1018236
version: 1
top level: BBa_I14006
access: public
definition: https://synbiohub.org/public/igem/BBa_C0077/1

BBa_C0077
type: SequenceAnnotation
persistent identity: annotation1018236
display id: annotation1018236
version: 1
top level: BBa_I14006
location: https://synbiohub.org/public/igem/BBa_I14006/annotation1018236/range1018236/1
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orientation: inline

BBa_B0030
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display id: range1018225
version: 1
direction: direction/forward
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orientation: inline

BBa_E0615
type: ComponentDefinition
persistent identity: BBa E0615
display id: BBa_E0615
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: I0500.Q04530.E0430
creator: cconboy
created: 2004-05-13T11:00:00Z
modified: 2015-08-31T04:07:26Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_1_
m user id: 0
owner id: 60
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
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display id: BBa_J11002
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was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: improved pulse generator/PI controller
creator: ingalls
created: 2005-06-20T11:00:00Z
modified: 2015-08-31T04:08:25Z
discontinued: false
dominant: false
experience: experience/None
group u list: _38_
m user id: 0
owner id: 337
owning group id: 38
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: input is luxI direct negative feedback by CI, integral feedback through p22 CII (in concert with part BBa_J1103). Generates bursts of gfp activity in response to step increases in luxI concentration. Should exhibit perfect(?!) adaptation.
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owned by: myers
star: false
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component: BBa_E0040
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was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Lambda cI QPI regulated by LacI with mCherry
creator: ytwang
created: 2005-06-27T11:00:00Z
modified: 2015-08-31T04:08:19Z
discontinued: true
dominant: false
experience: experience/None
group u list: _20_
m user id: 0
owner id: 340
owning group id: 20
sample status: Discontinued
status: status/Deleted
bookmark: false
mutable description: -- No description --
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star: false
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modified: 2015-08-31T04:07:44Z
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was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Plac . TetR
creator: CC Signalling
created: 2004-07-18T11:00:00Z
modified: 2015-05-08T01:14:33Z
discontinued: false
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modified: 2015-08-31T04:07:32Z
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dominant: false
experience: experience/None
group u list: _6_
m user id: 0
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bookmark: false
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BBa_I14025
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persistent identity: BBa I14025
display id: BBa_I14025
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: C12, C4 Router (strong RBS)
creator: Vikram Vijayan, Allen Hsu, Lawrence Fomundam
created: 2004-08-01T11:00:00Z
modified: 2015-08-31T04:07:37Z
discontinued: false
dominant: false
experience: experience/None
group u list: _4_
m user id: 0
owner id: 171
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sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: p(Las) TetO, Strong RBS, RhlI + LVA
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owned by: myers
star: false
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BBa_B0034
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display id: annotation964455
version: 1
top level: BBa_E0605
location: https://synbiohub.org/public/igem/BBa_E0605/annotation964455/range964455/1
component: BBa_B0034

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type: Range
persistent identity: range964455
display id: range964455
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direction: direction/forward
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orientation: inline

BBa_B0012
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version: 1
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display id: range964544
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end: 3072
orientation: inline

BBa_C0053
type: SequenceAnnotation
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display id: annotation964467
version: 1
top level: BBa_E0605
location: https://synbiohub.org/public/igem/BBa_E0605/annotation964467/range964467/1
component: BBa_C0053

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type: Range
persistent identity: range964467
display id: range964467
version: 1
direction: direction/forward
top level: BBa_E0605
start: 1237
end: 1923
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation964475
display id: annotation964475
version: 1
top level: BBa_E0605
location: https://synbiohub.org/public/igem/BBa_E0605/annotation964475/range964475/1
component: BBa_B0010

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persistent identity: range964475
display id: range964475
version: 1
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start: 1957
end: 2036
orientation: inline

BBa_E0032
type: SequenceAnnotation
persistent identity: annotation964526
display id: annotation964526
version: 1
top level: BBa_E0605
location: https://synbiohub.org/public/igem/BBa_E0605/annotation964526/range964526/1
component: BBa_E0032

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type: Range
persistent identity: range964526
display id: range964526
version: 1
direction: direction/forward
top level: BBa_E0605
start: 2174
end: 2935
orientation: inline

BBa_I0500
type: SequenceAnnotation
persistent identity: annotation964450
display id: annotation964450
version: 1
top level: BBa_E0605
location: https://synbiohub.org/public/igem/BBa_E0605/annotation964450/range964450/1
component: BBa_I0500

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type: Range
persistent identity: range964450
display id: range964450
version: 1
direction: direction/forward
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start: 1
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orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation964485
display id: annotation964485
version: 1
top level: BBa_E0605
location: https://synbiohub.org/public/igem/BBa_E0605/annotation964485/range964485/1
component: BBa_B0012

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type: Range
persistent identity: range964485
display id: range964485
version: 1
direction: direction/forward
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start: 2045
end: 2085
orientation: inline

BBa_I12055
type: ComponentDefinition
persistent identity: BBa I12055
display id: BBa_I12055
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: A protein (lambda cI) subassembly for modified BL oscillator based on I12006
creator: endelman
created: 2004-09-02T11:00:00Z
modified: 2015-08-31T04:07:31Z
discontinued: false
dominant: false
experience: experience/None
group u list: _3_
m user id: 0
owner id: 145
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This part has POPS output to connect with reporter. It is based on I12006.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_C0051
component: BBa_B0034
component: BBa_I12006
component: BBa_B0010
component: BBa_B0012
sequence annotation: BBa_B0012
sequence annotation: BBa_I12006
sequence annotation: BBa_B0034
sequence annotation: BBa_B0010
sequence annotation: BBa_C0051
sequence: https://synbiohub.org/public/igem/BBa_I12055_sequence/1

BBa_C0051
type: Component
persistent identity: component1241546
display id: component1241546
version: 1
top level: BBa_I12055
access: public
definition: https://synbiohub.org/public/igem/BBa_C0051/1

BBa_B0034
type: Component
persistent identity: component1241536
display id: component1241536
version: 1
top level: BBa_I12055
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_I12006
type: Component
persistent identity: component1241582
display id: component1241582
version: 1
top level: BBa_I12055
access: public
definition: https://synbiohub.org/public/igem/BBa_I12006/1

BBa_I12006
type: Component
persistent identity: component1241531
display id: component1241531
version: 1
top level: BBa_I12055
access: public
definition: https://synbiohub.org/public/igem/BBa_I12006/1

BBa_B0010
type: Component
persistent identity: component1241552
display id: component1241552
version: 1
top level: BBa_I12055
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0012
type: Component
persistent identity: component1241562
display id: component1241562
version: 1
top level: BBa_I12055
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1241562
display id: annotation1241562
version: 1
top level: BBa_I12055
location: https://synbiohub.org/public/igem/BBa_I12055/annotation1241562/range1241562/1
component: BBa_B0012

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type: Range
persistent identity: range1241562
display id: range1241562
version: 1
direction: direction/forward
top level: BBa_I12055
start: 980
end: 1020
orientation: inline

BBa_I12006
type: SequenceAnnotation
persistent identity: annotation1241582
display id: annotation1241582
version: 1
top level: BBa_I12055
location: https://synbiohub.org/public/igem/BBa_I12055/annotation1241582/range1241582/1
component: BBa_I12006

https://synbiohub.org/public/igem/BBa_I12055/annotation1241582/range1241582/1
type: Range
persistent identity: range1241582
display id: range1241582
version: 1
direction: direction/forward
top level: BBa_I12055
start: 1029
end: 1110
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1241536
display id: annotation1241536
version: 1
top level: BBa_I12055
location: https://synbiohub.org/public/igem/BBa_I12055/annotation1241536/range1241536/1
component: BBa_B0034

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persistent identity: range1241536
display id: range1241536
version: 1
direction: direction/forward
top level: BBa_I12055
start: 91
end: 102
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1241552
display id: annotation1241552
version: 1
top level: BBa_I12055
location: https://synbiohub.org/public/igem/BBa_I12055/annotation1241552/range1241552/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I12055/annotation1241552/range1241552/1
type: Range
persistent identity: range1241552
display id: range1241552
version: 1
direction: direction/forward
top level: BBa_I12055
start: 892
end: 971
orientation: inline

BBa_I12006
type: SequenceAnnotation
persistent identity: annotation1241531
display id: annotation1241531
version: 1
top level: BBa_I12055
location: https://synbiohub.org/public/igem/BBa_I12055/annotation1241531/range1241531/1
component: BBa_I12006

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type: Range
persistent identity: range1241531
display id: range1241531
version: 1
direction: direction/forward
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start: 1
end: 82
orientation: inline

BBa_C0051
type: SequenceAnnotation
persistent identity: annotation1241546
display id: annotation1241546
version: 1
top level: BBa_I12055
location: https://synbiohub.org/public/igem/BBa_I12055/annotation1241546/range1241546/1
component: BBa_C0051

https://synbiohub.org/public/igem/BBa_I12055/annotation1241546/range1241546/1
type: Range
persistent identity: range1241546
display id: range1241546
version: 1
direction: direction/forward
top level: BBa_I12055
start: 109
end: 858
orientation: inline

BBa_E0614
type: ComponentDefinition
persistent identity: BBa E0614
display id: BBa_E0614
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: I0500.Q04520.E0430
creator: cconboy
created: 2004-05-13T11:00:00Z
modified: 2015-08-31T04:07:26Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_1_
m user id: 0
owner id: 60
owning group id: 7
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_R0052
component: BBa_B0034
component: BBa_I0500
component: BBa_B0012
component: BBa_B0010
component: BBa_E0030
component: BBa_C0052
sequence annotation: BBa_B0010
sequence annotation: BBa_I0500
sequence annotation: BBa_B0034
sequence annotation: BBa_B0012
sequence annotation: BBa_R0052
sequence annotation: BBa_C0052
sequence annotation: BBa_E0030
sequence: https://synbiohub.org/public/igem/BBa_E0614_sequence/1

BBa_R0052
type: Component
persistent identity: component964912
display id: component964912
version: 1
top level: BBa_E0614
access: public
definition: https://synbiohub.org/public/igem/BBa_R0052/1

BBa_B0034
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persistent identity: component964863
display id: component964863
version: 1
top level: BBa_E0614
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0034
type: Component
persistent identity: component964922
display id: component964922
version: 1
top level: BBa_E0614
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_I0500
type: Component
persistent identity: component964858
display id: component964858
version: 1
top level: BBa_E0614
access: public
definition: https://synbiohub.org/public/igem/BBa_I0500/1

BBa_B0012
type: Component
persistent identity: component964890
display id: component964890
version: 1
top level: BBa_E0614
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0010
type: Component
persistent identity: component964929
display id: component964929
version: 1
top level: BBa_E0614
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0012
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persistent identity: component964939
display id: component964939
version: 1
top level: BBa_E0614
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0010
type: Component
persistent identity: component964880
display id: component964880
version: 1
top level: BBa_E0614
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_E0030
type: Component
persistent identity: component964924
display id: component964924
version: 1
top level: BBa_E0614
access: public
definition: https://synbiohub.org/public/igem/BBa_E0030/1

BBa_C0052
type: Component
persistent identity: component964873
display id: component964873
version: 1
top level: BBa_E0614
access: public
definition: https://synbiohub.org/public/igem/BBa_C0052/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation964929
display id: annotation964929
version: 1
top level: BBa_E0614
location: https://synbiohub.org/public/igem/BBa_E0614/annotation964929/range964929/1
component: BBa_B0010

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type: Range
persistent identity: range964929
display id: range964929
version: 1
direction: direction/forward
top level: BBa_E0614
start: 2879
end: 2958
orientation: inline

BBa_I0500
type: SequenceAnnotation
persistent identity: annotation964858
display id: annotation964858
version: 1
top level: BBa_E0614
location: https://synbiohub.org/public/igem/BBa_E0614/annotation964858/range964858/1
component: BBa_I0500

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type: Range
persistent identity: range964858
display id: range964858
version: 1
direction: direction/forward
top level: BBa_E0614
start: 1
end: 1210
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation964922
display id: annotation964922
version: 1
top level: BBa_E0614
location: https://synbiohub.org/public/igem/BBa_E0614/annotation964922/range964922/1
component: BBa_B0034

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type: Range
persistent identity: range964922
display id: range964922
version: 1
direction: direction/forward
top level: BBa_E0614
start: 2130
end: 2141
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation964939
display id: annotation964939
version: 1
top level: BBa_E0614
location: https://synbiohub.org/public/igem/BBa_E0614/annotation964939/range964939/1
component: BBa_B0012

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type: Range
persistent identity: range964939
display id: range964939
version: 1
direction: direction/forward
top level: BBa_E0614
start: 2967
end: 3007
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation964863
display id: annotation964863
version: 1
top level: BBa_E0614
location: https://synbiohub.org/public/igem/BBa_E0614/annotation964863/range964863/1
component: BBa_B0034

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type: Range
persistent identity: range964863
display id: range964863
version: 1
direction: direction/forward
top level: BBa_E0614
start: 1219
end: 1230
orientation: inline

BBa_R0052
type: SequenceAnnotation
persistent identity: annotation964912
display id: annotation964912
version: 1
top level: BBa_E0614
location: https://synbiohub.org/public/igem/BBa_E0614/annotation964912/range964912/1
component: BBa_R0052

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type: Range
persistent identity: range964912
display id: range964912
version: 1
direction: direction/forward
top level: BBa_E0614
start: 2076
end: 2121
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation964890
display id: annotation964890
version: 1
top level: BBa_E0614
location: https://synbiohub.org/public/igem/BBa_E0614/annotation964890/range964890/1
component: BBa_B0012

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type: Range
persistent identity: range964890
display id: range964890
version: 1
direction: direction/forward
top level: BBa_E0614
start: 2027
end: 2067
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation964880
display id: annotation964880
version: 1
top level: BBa_E0614
location: https://synbiohub.org/public/igem/BBa_E0614/annotation964880/range964880/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_E0614/annotation964880/range964880/1
type: Range
persistent identity: range964880
display id: range964880
version: 1
direction: direction/forward
top level: BBa_E0614
start: 1939
end: 2018
orientation: inline

BBa_C0052
type: SequenceAnnotation
persistent identity: annotation964873
display id: annotation964873
version: 1
top level: BBa_E0614
location: https://synbiohub.org/public/igem/BBa_E0614/annotation964873/range964873/1
component: BBa_C0052

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type: Range
persistent identity: range964873
display id: range964873
version: 1
direction: direction/forward
top level: BBa_E0614
start: 1237
end: 1905
orientation: inline

BBa_E0030
type: SequenceAnnotation
persistent identity: annotation964924
display id: annotation964924
version: 1
top level: BBa_E0614
location: https://synbiohub.org/public/igem/BBa_E0614/annotation964924/range964924/1
component: BBa_E0030

https://synbiohub.org/public/igem/BBa_E0614/annotation964924/range964924/1
type: Range
persistent identity: range964924
display id: range964924
version: 1
direction: direction/forward
top level: BBa_E0614
start: 2148
end: 2870
orientation: inline

BBa_I11063
type: ComponentDefinition
persistent identity: BBa I11063
display id: BBa_I11063
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: P21 half bit
creator: bu
created: 2004-09-07T11:00:00Z
modified: 2015-08-31T04:07:30Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2_
m user id: 0
owner id: 102
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: The P21 half bit uses integrase/excisionase from P21 and attP/attB (rev comp) from HK022. The attachment sites are flanking a unidirectional terminator. This half bit is designed to be used with the HK022 half bit (BBa_I11062)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: BamHI
sequence annotation: stop
sequence annotation: Integrase p21 (BBa_I11050)
sequence annotation: SalI
sequence annotation: RBS
sequence annotation: spacer
sequence annotation: XbaI
sequence annotation: HK022 attB rev comp (BBa_I11042)
sequence annotation: BBa_I11013 terminator
sequence annotation: SacII
sequence annotation: MluI
sequence annotation: HK022 attP attachment site (BBa_I11043)
sequence annotation: ApaI
sequence annotation: LVA
sequence annotation: Excisionase p21 (BBa_I11051)
sequence annotation: AAV
sequence: https://synbiohub.org/public/igem/BBa_I11063_sequence/1

BamHI
type: SequenceAnnotation
persistent identity: annotation1199640
display id: annotation1199640
version: 1
top level: BBa_I11063
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I11063/annotation1199640/range1199640/1

https://synbiohub.org/public/igem/BBa_I11063/annotation1199640/range1199640/1
type: Range
persistent identity: range1199640
display id: range1199640
version: 1
direction: direction/forward
top level: BBa_I11063
start: 1651
end: 1656
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1198455
display id: annotation1198455
version: 1
top level: BBa_I11063
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_I11063/annotation1198455/range1198455/1

https://synbiohub.org/public/igem/BBa_I11063/annotation1198455/range1198455/1
type: Range
persistent identity: range1198455
display id: range1198455
version: 1
direction: direction/forward
top level: BBa_I11063
start: 1197
end: 1202
orientation: inline

Integrase p21 (BBa_I11050)
type: SequenceAnnotation
persistent identity: annotation1198266
display id: annotation1198266
version: 1
top level: BBa_I11063
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_I11063/annotation1198266/range1198266/1

https://synbiohub.org/public/igem/BBa_I11063/annotation1198266/range1198266/1
type: Range
persistent identity: range1198266
display id: range1198266
version: 1
direction: direction/forward
top level: BBa_I11063
start: 24
end: 1163
orientation: inline

SalI
type: SequenceAnnotation
persistent identity: annotation1197876
display id: annotation1197876
version: 1
top level: BBa_I11063
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I11063/annotation1197876/range1197876/1

https://synbiohub.org/public/igem/BBa_I11063/annotation1197876/range1197876/1
type: Range
persistent identity: range1197876
display id: range1197876
version: 1
direction: direction/forward
top level: BBa_I11063
start: 4
end: 9
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation1199680
display id: annotation1199680
version: 1
top level: BBa_I11063
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_I11063/annotation1199680/range1199680/1

https://synbiohub.org/public/igem/BBa_I11063/annotation1199680/range1199680/1
type: Range
persistent identity: range1199680
display id: range1199680
version: 1
direction: direction/forward
top level: BBa_I11063
start: 1657
end: 1662
orientation: inline

spacer
type: SequenceAnnotation
persistent identity: annotation1199504
display id: annotation1199504
version: 1
top level: BBa_I11063
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I11063/annotation1199504/range1199504/1

https://synbiohub.org/public/igem/BBa_I11063/annotation1199504/range1199504/1
type: Range
persistent identity: range1199504
display id: range1199504
version: 1
direction: direction/forward
top level: BBa_I11063
start: 1594
end: 1596
orientation: inline

XbaI
type: SequenceAnnotation
persistent identity: annotation1199846
display id: annotation1199846
version: 1
top level: BBa_I11063
role: SO:0000110
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spacer
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HK022 attB rev comp (BBa_I11042)
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BBa_I11013 terminator
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MluI
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HK022 attP attachment site (BBa_I11043)
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ApaI
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LVA
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spacer
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Excisionase p21 (BBa_I11051)
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AAV
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RBS
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spacer
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spacer
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BBa_I13543
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display id: BBa_I13543
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was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Intermediate in two promoter screening plasmid
creator: Josh Michener
created: 2005-08-21T11:00:00Z
modified: 2015-08-31T04:07:35Z
discontinued: false
dominant: false
group u list: _11_
m user id: 0
owner id: 253
owning group id: 6
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: -- No description --
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star: false
type: DnaRegion
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BBa_I13457
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BBa_I0500
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BBa_I14051
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version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: C12, CI Router (weak RBS)
creator: Vikram Vijayan, Allen Hsu, Lawrence Fomundam
created: 2004-08-03T11:00:00Z
modified: 2015-08-31T04:07:38Z
discontinued: false
dominant: false
experience: experience/None
group u list: _4_
m user id: 0
owner id: 171
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: p(Las), Weak RBS, Lambda CI + LVA
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
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BBa_B0031
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BBa_R0079
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BBa_B0031
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BBa_I13403
type: ComponentDefinition
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display id: BBa_I13403
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: YFP + Pbad CFP
creator: jkm
created: 2005-04-25T11:00:00Z
modified: 2015-08-31T04:07:33Z
discontinued: false
dominant: false
experience: experience/None
group u list: _6_
m user id: 0
owner id: 253
owning group id: 6
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Intermediate in screening plasmid construction
owned by: james
owned by: myers
star: false
type: DnaRegion
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role: partType/Composite
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component: BBa_B0012
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BBa_E0020
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access: public
definition: https://synbiohub.org/public/igem/BBa_E0020/1

BBa_B0010
type: Component
persistent identity: component1480405
display id: component1480405
version: 1
top level: BBa_I13403
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_I0500
type: Component
persistent identity: component1480393
display id: component1480393
version: 1
top level: BBa_I13403
access: public
definition: https://synbiohub.org/public/igem/BBa_I0500/1

BBa_B0012
type: Component
persistent identity: component1480415
display id: component1480415
version: 1
top level: BBa_I13403
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_E0030
type: Component
persistent identity: component1480373
display id: component1480373
version: 1
top level: BBa_I13403
access: public
definition: https://synbiohub.org/public/igem/BBa_E0030/1

BBa_B0012
type: Component
persistent identity: component1480388
display id: component1480388
version: 1
top level: BBa_I13403
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0034
type: Component
persistent identity: component1480398
display id: component1480398
version: 1
top level: BBa_I13403
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0010
type: Component
persistent identity: component1480378
display id: component1480378
version: 1
top level: BBa_I13403
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1480398
display id: annotation1480398
version: 1
top level: BBa_I13403
location: https://synbiohub.org/public/igem/BBa_I13403/annotation1480398/range1480398/1
component: BBa_B0034

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type: Range
persistent identity: range1480398
display id: range1480398
version: 1
direction: direction/forward
top level: BBa_I13403
start: 2087
end: 2098
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1480388
display id: annotation1480388
version: 1
top level: BBa_I13403
location: https://synbiohub.org/public/igem/BBa_I13403/annotation1480388/range1480388/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I13403/annotation1480388/range1480388/1
type: Range
persistent identity: range1480388
display id: range1480388
version: 1
direction: direction/forward
top level: BBa_I13403
start: 820
end: 860
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1480415
display id: annotation1480415
version: 1
top level: BBa_I13403
location: https://synbiohub.org/public/igem/BBa_I13403/annotation1480415/range1480415/1
component: BBa_B0012

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type: Range
persistent identity: range1480415
display id: range1480415
version: 1
direction: direction/forward
top level: BBa_I13403
start: 2924
end: 2964
orientation: inline

BBa_E0030
type: SequenceAnnotation
persistent identity: annotation1480373
display id: annotation1480373
version: 1
top level: BBa_I13403
location: https://synbiohub.org/public/igem/BBa_I13403/annotation1480373/range1480373/1
component: BBa_E0030

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type: Range
persistent identity: range1480373
display id: range1480373
version: 1
direction: direction/forward
top level: BBa_I13403
start: 1
end: 723
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1480405
display id: annotation1480405
version: 1
top level: BBa_I13403
location: https://synbiohub.org/public/igem/BBa_I13403/annotation1480405/range1480405/1
component: BBa_B0010

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type: Range
persistent identity: range1480405
display id: range1480405
version: 1
direction: direction/forward
top level: BBa_I13403
start: 2836
end: 2915
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1480378
display id: annotation1480378
version: 1
top level: BBa_I13403
location: https://synbiohub.org/public/igem/BBa_I13403/annotation1480378/range1480378/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I13403/annotation1480378/range1480378/1
type: Range
persistent identity: range1480378
display id: range1480378
version: 1
direction: direction/forward
top level: BBa_I13403
start: 732
end: 811
orientation: inline

BBa_E0020
type: SequenceAnnotation
persistent identity: annotation1480400
display id: annotation1480400
version: 1
top level: BBa_I13403
location: https://synbiohub.org/public/igem/BBa_I13403/annotation1480400/range1480400/1
component: BBa_E0020

https://synbiohub.org/public/igem/BBa_I13403/annotation1480400/range1480400/1
type: Range
persistent identity: range1480400
display id: range1480400
version: 1
direction: direction/forward
top level: BBa_I13403
start: 2105
end: 2827
orientation: inline

BBa_I0500
type: SequenceAnnotation
persistent identity: annotation1480393
display id: annotation1480393
version: 1
top level: BBa_I13403
location: https://synbiohub.org/public/igem/BBa_I13403/annotation1480393/range1480393/1
component: BBa_I0500

https://synbiohub.org/public/igem/BBa_I13403/annotation1480393/range1480393/1
type: Range
persistent identity: range1480393
display id: range1480393
version: 1
direction: direction/forward
top level: BBa_I13403
start: 869
end: 2078
orientation: inline

BBa_I14056
type: ComponentDefinition
persistent identity: BBa I14056
display id: BBa_I14056
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Tet Toggle (strong RBS, weaker RBS)
creator: Vikram Vijayan, Allen Hsu, Lawrence Fomundam
created: 2004-08-03T11:00:00Z
modified: 2015-08-31T04:07:38Z
discontinued: false
dominant: false
experience: experience/None
group u list: _4_
m user id: 0
owner id: 171
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: p(Ltet0-1), Strong RBS, LacI + LVA, Weaker RBS, Lambda CI + LVA, Strong RBS, EYFP + LVA
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_C0051
component: BBa_B0030
component: BBa_B0033
component: BBa_C0012
component: BBa_R0040
component: BBa_E0032
sequence annotation: BBa_B0030
sequence annotation: BBa_C0051
sequence annotation: BBa_E0032
sequence annotation: BBa_R0040
sequence annotation: BBa_B0033
sequence annotation: BBa_C0012
sequence: https://synbiohub.org/public/igem/BBa_I14056_sequence/1

BBa_C0051
type: Component
persistent identity: component1029463
display id: component1029463
version: 1
top level: BBa_I14056
access: public
definition: https://synbiohub.org/public/igem/BBa_C0051/1

BBa_B0030
type: Component
persistent identity: component1029431
display id: component1029431
version: 1
top level: BBa_I14056
access: public
definition: https://synbiohub.org/public/igem/BBa_B0030/1

BBa_B0033
type: Component
persistent identity: component1029452
display id: component1029452
version: 1
top level: BBa_I14056
access: public
definition: https://synbiohub.org/public/igem/BBa_B0033/1

BBa_C0012
type: Component
persistent identity: component1029442
display id: component1029442
version: 1
top level: BBa_I14056
access: public
definition: https://synbiohub.org/public/igem/BBa_C0012/1

BBa_B0030
type: Component
persistent identity: component1029472
display id: component1029472
version: 1
top level: BBa_I14056
access: public
definition: https://synbiohub.org/public/igem/BBa_B0030/1

BBa_R0040
type: Component
persistent identity: component1029420
display id: component1029420
version: 1
top level: BBa_I14056
access: public
definition: https://synbiohub.org/public/igem/BBa_R0040/1

BBa_E0032
type: Component
persistent identity: component1029485
display id: component1029485
version: 1
top level: BBa_I14056
access: public
definition: https://synbiohub.org/public/igem/BBa_E0032/1

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation1029431
display id: annotation1029431
version: 1
top level: BBa_I14056
location: https://synbiohub.org/public/igem/BBa_I14056/annotation1029431/range1029431/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_I14056/annotation1029431/range1029431/1
type: Range
persistent identity: range1029431
display id: range1029431
version: 1
direction: direction/forward
top level: BBa_I14056
start: 63
end: 77
orientation: inline

BBa_C0051
type: SequenceAnnotation
persistent identity: annotation1029463
display id: annotation1029463
version: 1
top level: BBa_I14056
location: https://synbiohub.org/public/igem/BBa_I14056/annotation1029463/range1029463/1
component: BBa_C0051

https://synbiohub.org/public/igem/BBa_I14056/annotation1029463/range1029463/1
type: Range
persistent identity: range1029463
display id: range1029463
version: 1
direction: direction/forward
top level: BBa_I14056
start: 1262
end: 2011
orientation: inline

BBa_E0032
type: SequenceAnnotation
persistent identity: annotation1029485
display id: annotation1029485
version: 1
top level: BBa_I14056
location: https://synbiohub.org/public/igem/BBa_I14056/annotation1029485/range1029485/1
component: BBa_E0032

https://synbiohub.org/public/igem/BBa_I14056/annotation1029485/range1029485/1
type: Range
persistent identity: range1029485
display id: range1029485
version: 1
direction: direction/forward
top level: BBa_I14056
start: 2066
end: 2827
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation1029472
display id: annotation1029472
version: 1
top level: BBa_I14056
location: https://synbiohub.org/public/igem/BBa_I14056/annotation1029472/range1029472/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_I14056/annotation1029472/range1029472/1
type: Range
persistent identity: range1029472
display id: range1029472
version: 1
direction: direction/forward
top level: BBa_I14056
start: 2045
end: 2059
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1029420
display id: annotation1029420
version: 1
top level: BBa_I14056
location: https://synbiohub.org/public/igem/BBa_I14056/annotation1029420/range1029420/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_I14056/annotation1029420/range1029420/1
type: Range
persistent identity: range1029420
display id: range1029420
version: 1
direction: direction/forward
top level: BBa_I14056
start: 1
end: 54
orientation: inline

BBa_B0033
type: SequenceAnnotation
persistent identity: annotation1029452
display id: annotation1029452
version: 1
top level: BBa_I14056
location: https://synbiohub.org/public/igem/BBa_I14056/annotation1029452/range1029452/1
component: BBa_B0033

https://synbiohub.org/public/igem/BBa_I14056/annotation1029452/range1029452/1
type: Range
persistent identity: range1029452
display id: range1029452
version: 1
direction: direction/forward
top level: BBa_I14056
start: 1245
end: 1255
orientation: inline

BBa_C0012
type: SequenceAnnotation
persistent identity: annotation1029442
display id: annotation1029442
version: 1
top level: BBa_I14056
location: https://synbiohub.org/public/igem/BBa_I14056/annotation1029442/range1029442/1
component: BBa_C0012

https://synbiohub.org/public/igem/BBa_I14056/annotation1029442/range1029442/1
type: Range
persistent identity: range1029442
display id: range1029442
version: 1
direction: direction/forward
top level: BBa_I14056
start: 84
end: 1211
orientation: inline

BBa_K283040
type: ComponentDefinition
persistent identity: BBa K283040
display id: BBa_K283040
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: invasin (1-625aa)_RFP-terminators
creator: Shi Lei, Nelson Chu, W Ling
created: 2009-10-15T11:00:00Z
modified: 2015-05-08T01:11:48Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _383_
m user id: 0
owner id: 5494
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This part generates the chez protein which is responsible for the dephosphorylation of cheY protein in bacteria flagella movement. A 6-histidine tail is added to the chez coding region. The pTet promoter can be repressed by the TetR repressor protein(C0040). It is constitutively on without the presence of TetR repressor.
mutable notes: BBa_K283040
mutable provenance: From the registry
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: invasin(1-625)_ RFP
sequence annotation: T7 TE
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence annotation: stop
sequence annotation: polya
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_K283040_sequence/1

invasin(1-625)_ RFP
type: SequenceAnnotation
persistent identity: annotation2047995
display id: annotation2047995
version: 1
top level: BBa_K283040
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K283040/annotation2047995/range2047995/1

https://synbiohub.org/public/igem/BBa_K283040/annotation2047995/range2047995/1
type: Range
persistent identity: range2047995
display id: range2047995
version: 1
direction: direction/forward
top level: BBa_K283040
start: 1
end: 2960
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation2047999
display id: annotation2047999
version: 1
top level: BBa_K283040
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K283040/annotation2047999/range2047999/1

https://synbiohub.org/public/igem/BBa_K283040/annotation2047999/range2047999/1
type: Range
persistent identity: range2047999
display id: range2047999
version: 1
direction: direction/forward
top level: BBa_K283040
start: 3064
end: 3083
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2047996
display id: annotation2047996
version: 1
top level: BBa_K283040
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K283040/annotation2047996/range2047996/1

https://synbiohub.org/public/igem/BBa_K283040/annotation2047996/range2047996/1
type: Range
persistent identity: range2047996
display id: range2047996
version: 1
direction: direction/forward
top level: BBa_K283040
start: 2969
end: 3048
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation2047997
display id: annotation2047997
version: 1
top level: BBa_K283040
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K283040/annotation2047997/range2047997/1

https://synbiohub.org/public/igem/BBa_K283040/annotation2047997/range2047997/1
type: Range
persistent identity: range2047997
display id: range2047997
version: 1
direction: direction/forward
top level: BBa_K283040
start: 2980
end: 3023
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2048001
display id: annotation2048001
version: 1
top level: BBa_K283040
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K283040/annotation2048001/range2048001/1

https://synbiohub.org/public/igem/BBa_K283040/annotation2048001/range2048001/1
type: Range
persistent identity: range2048001
display id: range2048001
version: 1
direction: direction/forward
top level: BBa_K283040
start: 3090
end: 3090
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation2048000
display id: annotation2048000
version: 1
top level: BBa_K283040
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_K283040/annotation2048000/range2048000/1

https://synbiohub.org/public/igem/BBa_K283040/annotation2048000/range2048000/1
type: Range
persistent identity: range2048000
display id: range2048000
version: 1
direction: direction/forward
top level: BBa_K283040
start: 3084
end: 3097
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2047998
display id: annotation2047998
version: 1
top level: BBa_K283040
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K283040/annotation2047998/range2047998/1

https://synbiohub.org/public/igem/BBa_K283040/annotation2047998/range2047998/1
type: Range
persistent identity: range2047998
display id: range2047998
version: 1
direction: direction/forward
top level: BBa_K283040
start: 3057
end: 3097
orientation: inline

BBa_I13204
type: ComponentDefinition
persistent identity: BBa I13204
display id: BBa_I13204
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: I0500.I0460
creator: SMUG: CC signaling
created: 2004-08-30T11:00:00Z
modified: 2015-08-31T04:07:33Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_1_
m user id: 0
owner id: 60
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0012
component: BBa_C0060
component: BBa_B0010
component: BBa_B0034
component: BBa_I0500
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_B0034
sequence annotation: BBa_I0500
sequence annotation: BBa_C0060
sequence: https://synbiohub.org/public/igem/BBa_I13204_sequence/1

BBa_B0012
type: Component
persistent identity: component1061976
display id: component1061976
version: 1
top level: BBa_I13204
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_C0060
type: Component
persistent identity: component1061958
display id: component1061958
version: 1
top level: BBa_I13204
access: public
definition: https://synbiohub.org/public/igem/BBa_C0060/1

BBa_B0010
type: Component
persistent identity: component1061966
display id: component1061966
version: 1
top level: BBa_I13204
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0034
type: Component
persistent identity: component1061943
display id: component1061943
version: 1
top level: BBa_I13204
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_I0500
type: Component
persistent identity: component1061938
display id: component1061938
version: 1
top level: BBa_I13204
access: public
definition: https://synbiohub.org/public/igem/BBa_I0500/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1061966
display id: annotation1061966
version: 1
top level: BBa_I13204
location: https://synbiohub.org/public/igem/BBa_I13204/annotation1061966/range1061966/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I13204/annotation1061966/range1061966/1
type: Range
persistent identity: range1061966
display id: range1061966
version: 1
direction: direction/forward
top level: BBa_I13204
start: 2059
end: 2138
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1061976
display id: annotation1061976
version: 1
top level: BBa_I13204
location: https://synbiohub.org/public/igem/BBa_I13204/annotation1061976/range1061976/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I13204/annotation1061976/range1061976/1
type: Range
persistent identity: range1061976
display id: range1061976
version: 1
direction: direction/forward
top level: BBa_I13204
start: 2147
end: 2187
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1061943
display id: annotation1061943
version: 1
top level: BBa_I13204
location: https://synbiohub.org/public/igem/BBa_I13204/annotation1061943/range1061943/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I13204/annotation1061943/range1061943/1
type: Range
persistent identity: range1061943
display id: range1061943
version: 1
direction: direction/forward
top level: BBa_I13204
start: 1219
end: 1230
orientation: inline

BBa_I0500
type: SequenceAnnotation
persistent identity: annotation1061938
display id: annotation1061938
version: 1
top level: BBa_I13204
location: https://synbiohub.org/public/igem/BBa_I13204/annotation1061938/range1061938/1
component: BBa_I0500

https://synbiohub.org/public/igem/BBa_I13204/annotation1061938/range1061938/1
type: Range
persistent identity: range1061938
display id: range1061938
version: 1
direction: direction/forward
top level: BBa_I13204
start: 1
end: 1210
orientation: inline

BBa_C0060
type: SequenceAnnotation
persistent identity: annotation1061958
display id: annotation1061958
version: 1
top level: BBa_I13204
location: https://synbiohub.org/public/igem/BBa_I13204/annotation1061958/range1061958/1
component: BBa_C0060

https://synbiohub.org/public/igem/BBa_I13204/annotation1061958/range1061958/1
type: Range
persistent identity: range1061958
display id: range1061958
version: 1
direction: direction/forward
top level: BBa_I13204
start: 1237
end: 2025
orientation: inline

BBa_K283035
type: ComponentDefinition
persistent identity: BBa K283035
display id: BBa_K283035
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RFP-double terminators
creator: Shi Lei, Nelson Chu
created: 2009-10-15T11:00:00Z
modified: 2015-05-08T01:11:48Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _383_
m user id: 0
owner id: 5494
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: cI coding protein on plasmid backbone J61002
mutable notes: BBa_K283035
mutable provenance: From the registry
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_E1010
component: BBa_B0010
component: BBa_B0012
sequence annotation: BBa_B0012
sequence annotation: BBa_B0010
sequence annotation: BBa_E1010
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display id: component2246777
version: 1
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access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0012
type: Component
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display id: component2246779
version: 1
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access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2246779
display id: annotation2246779
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component: BBa_B0012

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component: BBa_B0010

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direction: direction/forward
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start: 715
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BBa_E1010
type: SequenceAnnotation
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display id: annotation2246776
version: 1
top level: BBa_K283035
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component: BBa_E1010

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persistent identity: range2246776
display id: range2246776
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BBa_I13279
type: ComponentDefinition
persistent identity: BBa I13279
display id: BBa_I13279
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PoPS->PoPS amplifier (LasR-based, HSL driven)
creator: Debra Lin, Cell-Cell Signaling SMUG
created: 2004-08-08T11:00:00Z
modified: 2015-08-31T04:07:33Z
discontinued: false
dominant: false
experience: experience/None
group u list: _6_
m user id: 0
owner id: 101
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: LasR protein generator upstream of pLas. Useful device for cell-cell signaling. Expected to require HSL for maximum PoPS out.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0010
component: BBa_R0079
component: BBa_C0179
component: BBa_B0012
component: BBa_B0034
sequence annotation: BBa_C0179
sequence annotation: BBa_B0034
sequence annotation: BBa_B0010
sequence annotation: BBa_R0079
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_I13279_sequence/1

BBa_B0010
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display id: component1035911
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access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_R0079
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access: public
definition: https://synbiohub.org/public/igem/BBa_R0079/1

BBa_C0179
type: Component
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definition: https://synbiohub.org/public/igem/BBa_C0179/1

BBa_B0012
type: Component
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display id: component1035921
version: 1
top level: BBa_I13279
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0034
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display id: component1035904
version: 1
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access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

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type: SequenceAnnotation
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component: BBa_C0179

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component: BBa_B0034

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direction: direction/forward
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BBa_I13280
type: ComponentDefinition
persistent identity: BBa I13280
display id: BBa_I13280
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pLac controlled receiver (LuxR-based, HSL driven)
creator: Barry Canton
created: 2004-08-08T11:00:00Z
modified: 2015-08-31T04:07:33Z
discontinued: true
dominant: false
experience: experience/None
group u list: _11_6_
m user id: 0
owner id: 135
owning group id: 7
sample status: Discontinued
status: status/Deleted
bookmark: false
mutable description: Composite based on the I13262 PoPS amplifier. Lac regulated, no downstream output device
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_R0011
component: BBa_B0034
component: BBa_R0062
component: BBa_B0012
component: BBa_B0010
component: BBa_C0062
sequence annotation: BBa_C0062
sequence annotation: BBa_R0011
sequence annotation: BBa_R0062
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sequence annotation: BBa_B0010
sequence annotation: BBa_B0034
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BBa_R0011
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version: 1
top level: BBa_I13280
access: public
definition: https://synbiohub.org/public/igem/BBa_R0011/1

BBa_B0034
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display id: range1039097
version: 1
direction: direction/forward
top level: BBa_I13280
start: 1
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component: BBa_R0062

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display id: range1039154
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direction: direction/forward
top level: BBa_I13280
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end: 1061
orientation: inline

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version: 1
top level: BBa_I13280
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component: BBa_B0012

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type: Range
persistent identity: range1039139
display id: range1039139
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direction: direction/forward
top level: BBa_I13280
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end: 998
orientation: inline

BBa_B0010
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component: BBa_B0010

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type: Range
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direction: direction/forward
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orientation: inline

BBa_B0034
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version: 1
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type: Range
persistent identity: range1039106
display id: range1039106
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direction: direction/forward
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start: 63
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orientation: inline

BBa_I14053
type: ComponentDefinition
persistent identity: BBa I14053
display id: BBa_I14053
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: C4, LacI Router (weak RBS)
creator: Vikram Vijayan, Allen Hsu, Lawrence Fomundam
created: 2004-08-03T11:00:00Z
modified: 2015-08-31T04:07:38Z
discontinued: false
dominant: false
experience: experience/None
group u list: _4_
m user id: 0
owner id: 171
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: p(Rhl), Weak RBS, LacI + LVA
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_C0012
component: BBa_B0031
component: BBa_I14017
sequence annotation: BBa_I14017
sequence annotation: BBa_B0031
sequence annotation: BBa_C0012
sequence: https://synbiohub.org/public/igem/BBa_I14053_sequence/1

BBa_C0012
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access: public
definition: https://synbiohub.org/public/igem/BBa_C0012/1

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display id: component1029253
version: 1
top level: BBa_I14053
access: public
definition: https://synbiohub.org/public/igem/BBa_B0031/1

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display id: component1029248
version: 1
top level: BBa_I14053
access: public
definition: https://synbiohub.org/public/igem/BBa_I14017/1

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persistent identity: annotation1029248
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version: 1
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persistent identity: range1029248
display id: range1029248
version: 1
direction: direction/forward
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start: 1
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orientation: inline

BBa_B0031
type: SequenceAnnotation
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display id: annotation1029253
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display id: range1029253
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direction: direction/forward
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location: https://synbiohub.org/public/igem/BBa_I14053/annotation1029263/range1029263/1
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type: Range
persistent identity: range1029263
display id: range1029263
version: 1
direction: direction/forward
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orientation: inline

BBa_J06911
type: ComponentDefinition
persistent identity: BBa J06911
display id: BBa_J06911
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LacI ts (mut 241) QPI regulated by lambda cI with EYFP
creator: Yves Wang
created: 2005-07-31T11:00:00Z
modified: 2015-08-31T04:08:19Z
discontinued: false
dominant: false
group u list: _20_
m user id: 0
owner id: 340
owning group id: 20
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_J06500
component: BBa_R0011
component: BBa_B0034
component: BBa_B0010
component: BBa_B0012
component: BBa_R0051
component: BBa_E0030
sequence annotation: BBa_R0051
sequence annotation: BBa_J06500
sequence annotation: BBa_B0010
sequence annotation: BBa_R0011
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sequence annotation: BBa_E0030
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_J06911_sequence/1

BBa_J06500
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version: 1
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access: public
definition: https://synbiohub.org/public/igem/BBa_J06500/1

BBa_R0011
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top level: BBa_J06911
access: public
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display id: component1599181
version: 1
top level: BBa_J06911
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0034
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display id: component1599233
version: 1
top level: BBa_J06911
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

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top level: BBa_J06911
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definition: https://synbiohub.org/public/igem/BBa_B0010/1

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definition: https://synbiohub.org/public/igem/BBa_B0010/1

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definition: https://synbiohub.org/public/igem/BBa_E0030/1

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access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

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display id: range1599200
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direction: direction/forward
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description: Test of BBa_R0011 (LacI regulated) using YFP
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modified: 2015-08-31T04:07:31Z
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experience: experience/None
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sample status: In stock
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bookmark: false
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owned by: myers
star: false
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description: completed 2 color temperature sensor
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created: 2005-07-26T11:00:00Z
modified: 2015-08-31T04:08:25Z
discontinued: false
dominant: false
group u list: _38_
m user id: 0
owner id: 341
owning group id: 38
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bookmark: false
mutable description: Combined two color temperature sensor
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owned by: myers
star: false
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star: false
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was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LacI ts (mut 265) QPI regulated by lambda cI with EYFP
creator: Yves Wang
created: 2005-07-31T11:00:00Z
modified: 2015-08-31T04:08:19Z
discontinued: false
dominant: false
group u list: _20_
m user id: 0
owner id: 340
owning group id: 20
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: -- No description --
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owned by: myers
star: false
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display id: BBa_I5610
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Elowitz Repressilator: QPI design
creator: Caitlin Conboy and Jennifer Braff
created: 2004-01-06T12:00:00Z
modified: 2015-08-31T04:07:42Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: The Elowitz Repressilator design, optimized for construction with quadpart inverters. YFP output device on same plasmid as repressilator.
owned by: james
owned by: myers
star: false
type: DnaRegion
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component: BBa_E0432
component: BBa_Q04400
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display id: range2237492
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BBa_R0040
type: SequenceAnnotation
persistent identity: annotation2237451
display id: annotation2237451
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component: BBa_R0040

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persistent identity: range2237451
display id: range2237451
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BBa_I14009
type: ComponentDefinition
persistent identity: BBa I14009
display id: BBa_I14009
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: B30, E32
creator: Vikram Vijayan, Allen Hsu, Lawrence Fomundam
created: 2004-07-22T11:00:00Z
modified: 2015-08-31T04:07:37Z
discontinued: false
dominant: true
experience: experience/None
group u list: _4_
m user id: 0
owner id: 166
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Strong RBS (B30), EYFP + LVA (E32) LINKS OK
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owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_E0032
component: BBa_B0030
sequence annotation: BBa_B0030
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access: public
definition: https://synbiohub.org/public/igem/BBa_E0032/1

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access: public
definition: https://synbiohub.org/public/igem/BBa_B0030/1

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BBa_I14011
type: ComponentDefinition
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display id: BBa_I14011
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: p(Cin) . strong RBS . LacI
creator: Aditi Shrivastava, Jiwon Lee, Sarah Welch
created: 2004-07-27T11:00:00Z
modified: 2015-08-31T04:07:37Z
discontinued: false
dominant: false
experience: experience/None
group u list: _4_
m user id: 0
owner id: 166
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: BBa_R0077 BBa_B0030 BBa_C0012
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owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0030
component: BBa_R0077
component: BBa_C0012
sequence annotation: BBa_C0012
sequence annotation: BBa_R0077
sequence annotation: BBa_B0030
sequence: https://synbiohub.org/public/igem/BBa_I14011_sequence/1

BBa_B0030
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persistent identity: component1018280
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version: 1
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access: public
definition: https://synbiohub.org/public/igem/BBa_B0030/1

BBa_R0077
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display id: component1018272
version: 1
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definition: https://synbiohub.org/public/igem/BBa_R0077/1

BBa_C0012
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access: public
definition: https://synbiohub.org/public/igem/BBa_C0012/1

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display id: BBa_I7003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: R0010.E0021.E0031
creator: Tom Knight
created: 2004-04-22T11:00:00Z
modified: 2015-08-31T04:07:44Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Wildtype lac promoter regulating expression of ECFP and EYFP (both +Clontech RBS, -LVA. Polycistronic message.)
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owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
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component: BBa_R0010
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sequence annotation: BBa_E0021
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BBa_E0021
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BBa_R0010
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BBa_E0031
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BBa_E0021
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version: 1
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component: BBa_E0021

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display id: range941420
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end: 948
orientation: inline

BBa_I14041
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persistent identity: BBa I14041
display id: BBa_I14041
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: weak RBS . penI
creator: Aditi Shrivastava, Jiwon Lee, Sarah Welch
created: 2004-08-03T11:00:00Z
modified: 2015-08-31T04:07:38Z
discontinued: false
dominant: false
experience: experience/None
group u list: _4_
m user id: 0
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sample status: Not in stock
status: status/Unavailable
bookmark: false
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owned by: myers
star: false
type: DnaRegion
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component: BBa_C0074
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BBa_C0074
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definition: https://synbiohub.org/public/igem/BBa_C0074/1

BBa_B0031
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version: 1
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access: public
definition: https://synbiohub.org/public/igem/BBa_B0031/1

BBa_C0074
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BBa_I14059
type: ComponentDefinition
persistent identity: BBa I14059
display id: BBa_I14059
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Constitutive LasR using P(Lac) IQ
creator: Vikram Vijayan, Allen Hsu, Lawrence Fomundam
created: 2004-08-04T11:00:00Z
modified: 2015-08-31T04:07:38Z
discontinued: false
dominant: false
experience: experience/None
group u list: _4_
m user id: 0
owner id: 171
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: p(Lac) IQ, Medium RBS, LasR + LVA
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_C0079
component: BBa_B0032
component: BBa_I14032
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sequence: https://synbiohub.org/public/igem/BBa_I14059_sequence/1

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access: public
definition: https://synbiohub.org/public/igem/BBa_C0079/1

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access: public
definition: https://synbiohub.org/public/igem/BBa_B0032/1

BBa_I14032
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display id: component1029588
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access: public
definition: https://synbiohub.org/public/igem/BBa_I14032/1

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type: SequenceAnnotation
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version: 1
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BBa_J05302
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persistent identity: BBa J05302
display id: BBa_J05302
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: R2 system A
creator: Alexander Roth
created: 2005-09-14T11:00:00Z
modified: 2015-08-31T04:08:15Z
discontinued: true
dominant: false
group u list: _34_
m user id: 0
owner id: 412
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sample status: Discontinued
status: status/Deleted
bookmark: false
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owned by: myers
star: false
type: DnaRegion
role: SO:0000804
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component: BBa_B0012
component: BBa_J05212
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sequence annotation: BBa_J05212
sequence annotation: BBa_J05102
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BBa_B0010
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definition: https://synbiohub.org/public/igem/BBa_B0010/1

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definition: https://synbiohub.org/public/igem/BBa_B0034/1

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display id: component1690210
version: 1
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definition: https://synbiohub.org/public/igem/BBa_J05102/1

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version: 1
direction: direction/forward
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start: 481
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orientation: inline

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display id: range1690210
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type: SequenceAnnotation
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display id: annotation1690206
version: 1
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persistent identity: range1690206
display id: range1690206
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BBa_I1100
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persistent identity: BBa I1100
display id: BBa_I1100
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: SBWB Ribosome Test System
creator: Jason Kelly
created: 2004-01-28T12:00:00Z
modified: 2015-08-31T04:07:30Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_R1051
component: BBa_B0015
component: BBa_B0035
component: BBa_C0100
component: BBa_C0080
component: BBa_I0500
component: BBa_C0101
sequence annotation: BBa_B0015
sequence annotation: BBa_C0080
sequence annotation: BBa_R1051
sequence annotation: BBa_C0101
sequence annotation: BBa_B0035
sequence annotation: BBa_C0100
sequence annotation: BBa_I0500
sequence: https://synbiohub.org/public/igem/BBa_I1100_sequence/1

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display id: component2233181
version: 1
top level: BBa_I1100
access: public
definition: https://synbiohub.org/public/igem/BBa_R1051/1

BBa_B0015
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persistent identity: component2233193
display id: component2233193
version: 1
top level: BBa_I1100
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_B0035
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version: 1
top level: BBa_I1100
access: public
definition: https://synbiohub.org/public/igem/BBa_B0035/1

BBa_B0015
type: Component
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display id: component2233163
version: 1
top level: BBa_I1100
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definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_C0100
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definition: https://synbiohub.org/public/igem/BBa_C0100/1

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definition: https://synbiohub.org/public/igem/BBa_C0080/1

BBa_R1051
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definition: https://synbiohub.org/public/igem/BBa_R1051/1

BBa_I0500
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persistent identity: component2233155
display id: component2233155
version: 1
top level: BBa_I1100
access: public
definition: https://synbiohub.org/public/igem/BBa_I0500/1

BBa_C0101
type: Component
persistent identity: component2233156
display id: component2233156
version: 1
top level: BBa_I1100
access: public
definition: https://synbiohub.org/public/igem/BBa_C0101/1

BBa_B0015
type: Component
persistent identity: component2233179
display id: component2233179
version: 1
top level: BBa_I1100
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2233179
display id: annotation2233179
version: 1
top level: BBa_I1100
location: https://synbiohub.org/public/igem/BBa_I1100/annotation2233179/range2233179/1
component: BBa_B0015

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type: Range
persistent identity: range2233179
display id: range2233179
version: 1
direction: direction/reverse
top level: BBa_I1100
start: 8358
end: 8486
orientation: inline

BBa_C0080
type: SequenceAnnotation
persistent identity: annotation2233172
display id: annotation2233172
version: 1
top level: BBa_I1100
location: https://synbiohub.org/public/igem/BBa_I1100/annotation2233172/range2233172/1
component: BBa_C0080

https://synbiohub.org/public/igem/BBa_I1100/annotation2233172/range2233172/1
type: Range
persistent identity: range2233172
display id: range2233172
version: 1
direction: direction/reverse
top level: BBa_I1100
start: 7410
end: 8349
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2233193
display id: annotation2233193
version: 1
top level: BBa_I1100
location: https://synbiohub.org/public/igem/BBa_I1100/annotation2233193/range2233193/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_I1100/annotation2233193/range2233193/1
type: Range
persistent identity: range2233193
display id: range2233193
version: 1
direction: direction/reverse
top level: BBa_I1100
start: 9241
end: 9369
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2233163
display id: annotation2233163
version: 1
top level: BBa_I1100
location: https://synbiohub.org/public/igem/BBa_I1100/annotation2233163/range2233163/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_I1100/annotation2233163/range2233163/1
type: Range
persistent identity: range2233163
display id: range2233163
version: 1
direction: direction/reverse
top level: BBa_I1100
start: 7218
end: 7346
orientation: inline

BBa_R1051
type: SequenceAnnotation
persistent identity: annotation2233165
display id: annotation2233165
version: 1
top level: BBa_I1100
location: https://synbiohub.org/public/igem/BBa_I1100/annotation2233165/range2233165/1
component: BBa_R1051

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type: Range
persistent identity: range2233165
display id: range2233165
version: 1
direction: direction/forward
top level: BBa_I1100
start: 7355
end: 7403
orientation: inline

BBa_C0101
type: SequenceAnnotation
persistent identity: annotation2233156
display id: annotation2233156
version: 1
top level: BBa_I1100
location: https://synbiohub.org/public/igem/BBa_I1100/annotation2233156/range2233156/1
component: BBa_C0101

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type: Range
persistent identity: range2233156
display id: range2233156
version: 1
direction: direction/reverse
top level: BBa_I1100
start: 1219
end: 7209
orientation: inline

BBa_B0035
type: SequenceAnnotation
persistent identity: annotation2233185
display id: annotation2233185
version: 1
top level: BBa_I1100
location: https://synbiohub.org/public/igem/BBa_I1100/annotation2233185/range2233185/1
component: BBa_B0035

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type: Range
persistent identity: range2233185
display id: range2233185
version: 1
direction: direction/reverse
top level: BBa_I1100
start: 8552
end: 8565
orientation: inline

BBa_C0100
type: SequenceAnnotation
persistent identity: annotation2233186
display id: annotation2233186
version: 1
top level: BBa_I1100
location: https://synbiohub.org/public/igem/BBa_I1100/annotation2233186/range2233186/1
component: BBa_C0100

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type: Range
persistent identity: range2233186
display id: range2233186
version: 1
direction: direction/reverse
top level: BBa_I1100
start: 8574
end: 9232
orientation: inline

BBa_I0500
type: SequenceAnnotation
persistent identity: annotation2233155
display id: annotation2233155
version: 1
top level: BBa_I1100
location: https://synbiohub.org/public/igem/BBa_I1100/annotation2233155/range2233155/1
component: BBa_I0500

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type: Range
persistent identity: range2233155
display id: range2233155
version: 1
direction: direction/reverse
top level: BBa_I1100
start: 1
end: 1210
orientation: inline

BBa_R1051
type: SequenceAnnotation
persistent identity: annotation2233181
display id: annotation2233181
version: 1
top level: BBa_I1100
location: https://synbiohub.org/public/igem/BBa_I1100/annotation2233181/range2233181/1
component: BBa_R1051

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type: Range
persistent identity: range2233181
display id: range2233181
version: 1
direction: direction/forward
top level: BBa_I1100
start: 8495
end: 8543
orientation: inline

BBa_J05313
type: ComponentDefinition
persistent identity: BBa J05313
display id: BBa_J05313
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: R2 state
creator: Alexander Roth
created: 2005-09-14T11:00:00Z
modified: 2015-08-31T04:08:15Z
discontinued: false
dominant: false
group u list: _34_
m user id: 0
owner id: 412
owning group id: 34
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_J05217
component: BBa_J05110
component: BBa_B0015
component: BBa_B0034
sequence annotation: BBa_B0034
sequence annotation: BBa_J05110
sequence annotation: BBa_J05217
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_J05313_sequence/1

BBa_J05217
type: Component
persistent identity: component2221765
display id: component2221765
version: 1
top level: BBa_J05313
access: public
definition: https://synbiohub.org/public/igem/BBa_J05217/1

BBa_J05110
type: Component
persistent identity: component2221775
display id: component2221775
version: 1
top level: BBa_J05313
access: public
definition: https://synbiohub.org/public/igem/BBa_J05110/1

BBa_B0015
type: Component
persistent identity: component2221782
display id: component2221782
version: 1
top level: BBa_J05313
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_B0034
type: Component
persistent identity: component2221767
display id: component2221767
version: 1
top level: BBa_J05313
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2221767
display id: annotation2221767
version: 1
top level: BBa_J05313
location: https://synbiohub.org/public/igem/BBa_J05313/annotation2221767/range2221767/1
component: BBa_B0034

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type: Range
persistent identity: range2221767
display id: range2221767
version: 1
direction: direction/reverse
top level: BBa_J05313
start: 50
end: 61
orientation: inline

BBa_J05110
type: SequenceAnnotation
persistent identity: annotation2221775
display id: annotation2221775
version: 1
top level: BBa_J05313
location: https://synbiohub.org/public/igem/BBa_J05313/annotation2221775/range2221775/1
component: BBa_J05110

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type: Range
persistent identity: range2221775
display id: range2221775
version: 1
direction: direction/reverse
top level: BBa_J05313
start: 68
end: 533
orientation: inline

BBa_J05217
type: SequenceAnnotation
persistent identity: annotation2221765
display id: annotation2221765
version: 1
top level: BBa_J05313
location: https://synbiohub.org/public/igem/BBa_J05313/annotation2221765/range2221765/1
component: BBa_J05217

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type: Range
persistent identity: range2221765
display id: range2221765
version: 1
direction: direction/reverse
top level: BBa_J05313
start: 1
end: 41
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2221782
display id: annotation2221782
version: 1
top level: BBa_J05313
location: https://synbiohub.org/public/igem/BBa_J05313/annotation2221782/range2221782/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_J05313/annotation2221782/range2221782/1
type: Range
persistent identity: range2221782
display id: range2221782
version: 1
direction: direction/reverse
top level: BBa_J05313
start: 542
end: 670
orientation: inline

BBa_J05312
type: ComponentDefinition
persistent identity: BBa J05312
display id: BBa_J05312
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: R3 state
creator: Alexander Roth
created: 2005-09-14T11:00:00Z
modified: 2015-08-31T04:08:15Z
discontinued: false
dominant: false
group u list: _34_
m user id: 0
owner id: 412
owning group id: 34
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0015
component: BBa_B0034
component: BBa_J05216
component: BBa_J05109
sequence annotation: BBa_B0015
sequence annotation: BBa_B0034
sequence annotation: BBa_J05109
sequence annotation: BBa_J05216
sequence: https://synbiohub.org/public/igem/BBa_J05312_sequence/1

BBa_B0015
type: Component
persistent identity: component2221764
display id: component2221764
version: 1
top level: BBa_J05312
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_B0034
type: Component
persistent identity: component2221749
display id: component2221749
version: 1
top level: BBa_J05312
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_J05216
type: Component
persistent identity: component2221747
display id: component2221747
version: 1
top level: BBa_J05312
access: public
definition: https://synbiohub.org/public/igem/BBa_J05216/1

BBa_J05109
type: Component
persistent identity: component2221757
display id: component2221757
version: 1
top level: BBa_J05312
access: public
definition: https://synbiohub.org/public/igem/BBa_J05109/1

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2221764
display id: annotation2221764
version: 1
top level: BBa_J05312
location: https://synbiohub.org/public/igem/BBa_J05312/annotation2221764/range2221764/1
component: BBa_B0015

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type: Range
persistent identity: range2221764
display id: range2221764
version: 1
direction: direction/reverse
top level: BBa_J05312
start: 515
end: 643
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2221749
display id: annotation2221749
version: 1
top level: BBa_J05312
location: https://synbiohub.org/public/igem/BBa_J05312/annotation2221749/range2221749/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_J05312/annotation2221749/range2221749/1
type: Range
persistent identity: range2221749
display id: range2221749
version: 1
direction: direction/reverse
top level: BBa_J05312
start: 50
end: 61
orientation: inline

BBa_J05109
type: SequenceAnnotation
persistent identity: annotation2221757
display id: annotation2221757
version: 1
top level: BBa_J05312
location: https://synbiohub.org/public/igem/BBa_J05312/annotation2221757/range2221757/1
component: BBa_J05109

https://synbiohub.org/public/igem/BBa_J05312/annotation2221757/range2221757/1
type: Range
persistent identity: range2221757
display id: range2221757
version: 1
direction: direction/reverse
top level: BBa_J05312
start: 68
end: 506
orientation: inline

BBa_J05216
type: SequenceAnnotation
persistent identity: annotation2221747
display id: annotation2221747
version: 1
top level: BBa_J05312
location: https://synbiohub.org/public/igem/BBa_J05312/annotation2221747/range2221747/1
component: BBa_J05216

https://synbiohub.org/public/igem/BBa_J05312/annotation2221747/range2221747/1
type: Range
persistent identity: range2221747
display id: range2221747
version: 1
direction: direction/reverse
top level: BBa_J05312
start: 1
end: 41
orientation: inline

BBa_I13721
type: ComponentDefinition
persistent identity: BBa I13721
display id: BBa_I13721
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CheB under Lac control with Elowitz RBS
creator: Victoria Chou, Kenneth Nesmith, Madeleine Sheldon-Dante
created: 2004-08-12T11:00:00Z
modified: 2015-08-31T04:07:36Z
discontinued: false
dominant: false
experience: experience/None
group u list: _6_
m user id: 0
owner id: 101
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0034
component: BBa_B0015
component: BBa_C0024
component: BBa_R0011
sequence annotation: BBa_R0011
sequence annotation: BBa_B0034
sequence annotation: BBa_C0024
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_I13721_sequence/1

BBa_B0034
type: Component
persistent identity: component2221641
display id: component2221641
version: 1
top level: BBa_I13721
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0015
type: Component
persistent identity: component2221651
display id: component2221651
version: 1
top level: BBa_I13721
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_C0024
type: Component
persistent identity: component2221644
display id: component2221644
version: 1
top level: BBa_I13721
access: public
definition: https://synbiohub.org/public/igem/BBa_C0024/1

BBa_R0011
type: Component
persistent identity: component2221635
display id: component2221635
version: 1
top level: BBa_I13721
access: public
definition: https://synbiohub.org/public/igem/BBa_R0011/1

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation2221635
display id: annotation2221635
version: 1
top level: BBa_I13721
location: https://synbiohub.org/public/igem/BBa_I13721/annotation2221635/range2221635/1
component: BBa_R0011

https://synbiohub.org/public/igem/BBa_I13721/annotation2221635/range2221635/1
type: Range
persistent identity: range2221635
display id: range2221635
version: 1
direction: direction/forward
top level: BBa_I13721
start: 1
end: 54
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2221641
display id: annotation2221641
version: 1
top level: BBa_I13721
location: https://synbiohub.org/public/igem/BBa_I13721/annotation2221641/range2221641/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I13721/annotation2221641/range2221641/1
type: Range
persistent identity: range2221641
display id: range2221641
version: 1
direction: direction/reverse
top level: BBa_I13721
start: 64
end: 75
orientation: inline

BBa_C0024
type: SequenceAnnotation
persistent identity: annotation2221644
display id: annotation2221644
version: 1
top level: BBa_I13721
location: https://synbiohub.org/public/igem/BBa_I13721/annotation2221644/range2221644/1
component: BBa_C0024

https://synbiohub.org/public/igem/BBa_I13721/annotation2221644/range2221644/1
type: Range
persistent identity: range2221644
display id: range2221644
version: 1
direction: direction/reverse
top level: BBa_I13721
start: 82
end: 1159
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2221651
display id: annotation2221651
version: 1
top level: BBa_I13721
location: https://synbiohub.org/public/igem/BBa_I13721/annotation2221651/range2221651/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_I13721/annotation2221651/range2221651/1
type: Range
persistent identity: range2221651
display id: range2221651
version: 1
direction: direction/reverse
top level: BBa_I13721
start: 1168
end: 1296
orientation: inline

BBa_K1379007
type: ComponentDefinition
persistent identity: BBa K1379007
display id: BBa_K1379007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: σx Generator + PcomFA-E0240
creator: Ng Siu Wang Edward, Jason Jang, Joo Min Jung
created: 2014-09-22T11:00:00Z
modified: 2015-05-08T01:10:12Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1756_
m user id: 0
owner id: 21235
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: SigmaX generator, SigmaX-inducible Phelicase encoding GFP
mutable notes: N/A
mutable provenance: Both comX gene and Phelicase are found in genomic sequence of Streptococcus pneumoniae NCTC7465 strain, while the RBS+GFP+DTT, downstream of PCelA, is BBa_E0240. The promoter encoding comX is BBa_K880005 and the double terminator for it is BBa_B0015
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Measurement
component: BBa_K1379001
component: BBa_E0240
component: BBa_K1379006
sequence annotation: BBa_K1379006
sequence annotation: BBa_E0240
sequence annotation: BBa_K1379001
sequence: https://synbiohub.org/public/igem/BBa_K1379007_sequence/1

BBa_K1379001
type: Component
persistent identity: component2421713
display id: component2421713
version: 1
top level: BBa_K1379007
access: public
definition: https://synbiohub.org/public/igem/BBa_K1379001/1

BBa_E0240
type: Component
persistent identity: component2421724
display id: component2421724
version: 1
top level: BBa_K1379007
access: public
definition: https://synbiohub.org/public/igem/BBa_E0240/1

BBa_K1379006
type: Component
persistent identity: component2421710
display id: component2421710
version: 1
top level: BBa_K1379007
access: public
definition: https://synbiohub.org/public/igem/BBa_K1379006/1

BBa_K1379006
type: SequenceAnnotation
persistent identity: annotation2421710
display id: annotation2421710
version: 1
top level: BBa_K1379007
location: https://synbiohub.org/public/igem/BBa_K1379007/annotation2421710/range2421710/1
component: BBa_K1379006

https://synbiohub.org/public/igem/BBa_K1379007/annotation2421710/range2421710/1
type: Range
persistent identity: range2421710
display id: range2421710
version: 1
direction: direction/reverse
top level: BBa_K1379007
start: 1
end: 711
orientation: inline

BBa_E0240
type: SequenceAnnotation
persistent identity: annotation2421724
display id: annotation2421724
version: 1
top level: BBa_K1379007
location: https://synbiohub.org/public/igem/BBa_K1379007/annotation2421724/range2421724/1
component: BBa_E0240

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type: Range
persistent identity: range2421724
display id: range2421724
version: 1
direction: direction/reverse
top level: BBa_K1379007
start: 888
end: 1763
orientation: inline

BBa_K1379001
type: SequenceAnnotation
persistent identity: annotation2421713
display id: annotation2421713
version: 1
top level: BBa_K1379007
location: https://synbiohub.org/public/igem/BBa_K1379007/annotation2421713/range2421713/1
component: BBa_K1379001

https://synbiohub.org/public/igem/BBa_K1379007/annotation2421713/range2421713/1
type: Range
persistent identity: range2421713
display id: range2421713
version: 1
direction: direction/reverse
top level: BBa_K1379007
start: 720
end: 879
orientation: inline

BBa_I11060
type: ComponentDefinition
persistent identity: BBa I11060
display id: BBa_I11060
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Lambda half bit
creator: mschomp
created: 2004-09-02T11:00:00Z
modified: 2015-08-31T04:07:30Z
discontinued: false
dominant: false
experience: experience/Issues
group u list: _2_
m user id: 0
owner id: 102
owning group id: 7
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: half bit contains integrase and excisionase coding regions for lambda phage and attachment sites for p22 int/xis proteins that flip a unidirecitonal terminator
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: KpnI
sequence annotation: Integrase lambda
sequence annotation: spacer
sequence annotation: BmtI
sequence annotation: AAV
sequence annotation: P22 attP (BBa_I11033)
sequence annotation: NotI
sequence annotation: RBS (BBa_I11010)
sequence annotation: Xis lambda
sequence annotation: ReverseTerminator (BBa_B0025)
sequence annotation: LVA
sequence annotation: XmaI
sequence annotation: stop
sequence annotation: P22 attB rev comp (BBa_I11032)
sequence annotation: AclI
sequence annotation: PvuI
sequence: https://synbiohub.org/public/igem/BBa_I11060_sequence/1

KpnI
type: SequenceAnnotation
persistent identity: annotation1197089
display id: annotation1197089
version: 1
top level: BBa_I11060
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I11060/annotation1197089/range1197089/1

https://synbiohub.org/public/igem/BBa_I11060/annotation1197089/range1197089/1
type: Range
persistent identity: range1197089
display id: range1197089
version: 1
direction: direction/forward
top level: BBa_I11060
start: 4
end: 9
orientation: inline

Integrase lambda
type: SequenceAnnotation
persistent identity: annotation1197093
display id: annotation1197093
version: 1
top level: BBa_I11060
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_I11060/annotation1197093/range1197093/1

https://synbiohub.org/public/igem/BBa_I11060/annotation1197093/range1197093/1
type: Range
persistent identity: range1197093
display id: range1197093
version: 1
direction: direction/forward
top level: BBa_I11060
start: 24
end: 1091
orientation: inline

spacer
type: SequenceAnnotation
persistent identity: annotation1197096
display id: annotation1197096
version: 1
top level: BBa_I11060
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I11060/annotation1197096/range1197096/1

https://synbiohub.org/public/igem/BBa_I11060/annotation1197096/range1197096/1
type: Range
persistent identity: range1197096
display id: range1197096
version: 1
direction: direction/forward
top level: BBa_I11060
start: 1131
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orientation: inline

BmtI
type: SequenceAnnotation
persistent identity: annotation1197109
display id: annotation1197109
version: 1
top level: BBa_I11060
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I11060/annotation1197109/range1197109/1

https://synbiohub.org/public/igem/BBa_I11060/annotation1197109/range1197109/1
type: Range
persistent identity: range1197109
display id: range1197109
version: 1
direction: direction/forward
top level: BBa_I11060
start: 1587
end: 1592
orientation: inline

spacer
type: SequenceAnnotation
persistent identity: annotation1197090
display id: annotation1197090
version: 1
top level: BBa_I11060
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I11060/annotation1197090/range1197090/1

https://synbiohub.org/public/igem/BBa_I11060/annotation1197090/range1197090/1
type: Range
persistent identity: range1197090
display id: range1197090
version: 1
direction: direction/forward
top level: BBa_I11060
start: 10
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orientation: inline

spacer
type: SequenceAnnotation
persistent identity: annotation1197102
display id: annotation1197102
version: 1
top level: BBa_I11060
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I11060/annotation1197102/range1197102/1

https://synbiohub.org/public/igem/BBa_I11060/annotation1197102/range1197102/1
type: Range
persistent identity: range1197102
display id: range1197102
version: 1
direction: direction/forward
top level: BBa_I11060
start: 1412
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orientation: inline

AAV
type: SequenceAnnotation
persistent identity: annotation1197113
display id: annotation1197113
version: 1
top level: BBa_I11060
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_I11060/annotation1197113/range1197113/1

https://synbiohub.org/public/igem/BBa_I11060/annotation1197113/range1197113/1
type: Range
persistent identity: range1197113
display id: range1197113
version: 1
direction: direction/forward
top level: BBa_I11060
start: 1820
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orientation: inline

spacer
type: SequenceAnnotation
persistent identity: annotation1197088
display id: annotation1197088
version: 1
top level: BBa_I11060
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I11060/annotation1197088/range1197088/1

https://synbiohub.org/public/igem/BBa_I11060/annotation1197088/range1197088/1
type: Range
persistent identity: range1197088
display id: range1197088
version: 1
direction: direction/forward
top level: BBa_I11060
start: 1
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orientation: inline

P22 attP (BBa_I11033)
type: SequenceAnnotation
persistent identity: annotation1197099
display id: annotation1197099
version: 1
top level: BBa_I11060
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I11060/annotation1197099/range1197099/1

https://synbiohub.org/public/igem/BBa_I11060/annotation1197099/range1197099/1
type: Range
persistent identity: range1197099
display id: range1197099
version: 1
direction: direction/forward
top level: BBa_I11060
start: 1143
end: 1402
orientation: inline

NotI
type: SequenceAnnotation
persistent identity: annotation1197116
display id: annotation1197116
version: 1
top level: BBa_I11060
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I11060/annotation1197116/range1197116/1

https://synbiohub.org/public/igem/BBa_I11060/annotation1197116/range1197116/1
type: Range
persistent identity: range1197116
display id: range1197116
version: 1
direction: direction/forward
top level: BBa_I11060
start: 1862
end: 1869
orientation: inline

RBS (BBa_I11010)
type: SequenceAnnotation
persistent identity: annotation1197091
display id: annotation1197091
version: 1
top level: BBa_I11060
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_I11060/annotation1197091/range1197091/1

https://synbiohub.org/public/igem/BBa_I11060/annotation1197091/range1197091/1
type: Range
persistent identity: range1197091
display id: range1197091
version: 1
direction: direction/forward
top level: BBa_I11060
start: 13
end: 18
orientation: inline

Xis lambda
type: SequenceAnnotation
persistent identity: annotation1197112
display id: annotation1197112
version: 1
top level: BBa_I11060
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_I11060/annotation1197112/range1197112/1

https://synbiohub.org/public/igem/BBa_I11060/annotation1197112/range1197112/1
type: Range
persistent identity: range1197112
display id: range1197112
version: 1
direction: direction/forward
top level: BBa_I11060
start: 1604
end: 1819
orientation: inline

ReverseTerminator (BBa_B0025)
type: SequenceAnnotation
persistent identity: annotation1197103
display id: annotation1197103
version: 1
top level: BBa_I11060
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_I11060/annotation1197103/range1197103/1

https://synbiohub.org/public/igem/BBa_I11060/annotation1197103/range1197103/1
type: Range
persistent identity: range1197103
display id: range1197103
version: 1
direction: direction/forward
top level: BBa_I11060
start: 1415
end: 1544
orientation: inline

spacer
type: SequenceAnnotation
persistent identity: annotation1197092
display id: annotation1197092
version: 1
top level: BBa_I11060
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I11060/annotation1197092/range1197092/1

https://synbiohub.org/public/igem/BBa_I11060/annotation1197092/range1197092/1
type: Range
persistent identity: range1197092
display id: range1197092
version: 1
direction: direction/forward
top level: BBa_I11060
start: 19
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orientation: inline

spacer
type: SequenceAnnotation
persistent identity: annotation1197117
display id: annotation1197117
version: 1
top level: BBa_I11060
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I11060/annotation1197117/range1197117/1

https://synbiohub.org/public/igem/BBa_I11060/annotation1197117/range1197117/1
type: Range
persistent identity: range1197117
display id: range1197117
version: 1
direction: direction/forward
top level: BBa_I11060
start: 1870
end: 1871
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation1197094
display id: annotation1197094
version: 1
top level: BBa_I11060
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_I11060/annotation1197094/range1197094/1

https://synbiohub.org/public/igem/BBa_I11060/annotation1197094/range1197094/1
type: Range
persistent identity: range1197094
display id: range1197094
version: 1
direction: direction/forward
top level: BBa_I11060
start: 1092
end: 1124
orientation: inline

XmaI
type: SequenceAnnotation
persistent identity: annotation1197105
display id: annotation1197105
version: 1
top level: BBa_I11060
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I11060/annotation1197105/range1197105/1

https://synbiohub.org/public/igem/BBa_I11060/annotation1197105/range1197105/1
type: Range
persistent identity: range1197105
display id: range1197105
version: 1
direction: direction/forward
top level: BBa_I11060
start: 1548
end: 1553
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1197114
display id: annotation1197114
version: 1
top level: BBa_I11060
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_I11060/annotation1197114/range1197114/1

https://synbiohub.org/public/igem/BBa_I11060/annotation1197114/range1197114/1
type: Range
persistent identity: range1197114
display id: range1197114
version: 1
direction: direction/forward
top level: BBa_I11060
start: 1853
end: 1858
orientation: inline

spacer
type: SequenceAnnotation
persistent identity: annotation1197115
display id: annotation1197115
version: 1
top level: BBa_I11060
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I11060/annotation1197115/range1197115/1

https://synbiohub.org/public/igem/BBa_I11060/annotation1197115/range1197115/1
type: Range
persistent identity: range1197115
display id: range1197115
version: 1
direction: direction/forward
top level: BBa_I11060
start: 1859
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orientation: inline

spacer
type: SequenceAnnotation
persistent identity: annotation1197111
display id: annotation1197111
version: 1
top level: BBa_I11060
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I11060/annotation1197111/range1197111/1

https://synbiohub.org/public/igem/BBa_I11060/annotation1197111/range1197111/1
type: Range
persistent identity: range1197111
display id: range1197111
version: 1
direction: direction/forward
top level: BBa_I11060
start: 1599
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orientation: inline

spacer
type: SequenceAnnotation
persistent identity: annotation1197100
display id: annotation1197100
version: 1
top level: BBa_I11060
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I11060/annotation1197100/range1197100/1

https://synbiohub.org/public/igem/BBa_I11060/annotation1197100/range1197100/1
type: Range
persistent identity: range1197100
display id: range1197100
version: 1
direction: direction/forward
top level: BBa_I11060
start: 1403
end: 1405
orientation: inline

P22 attB rev comp (BBa_I11032)
type: SequenceAnnotation
persistent identity: annotation1197107
display id: annotation1197107
version: 1
top level: BBa_I11060
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I11060/annotation1197107/range1197107/1

https://synbiohub.org/public/igem/BBa_I11060/annotation1197107/range1197107/1
type: Range
persistent identity: range1197107
display id: range1197107
version: 1
direction: direction/forward
top level: BBa_I11060
start: 1557
end: 1583
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1197095
display id: annotation1197095
version: 1
top level: BBa_I11060
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_I11060/annotation1197095/range1197095/1

https://synbiohub.org/public/igem/BBa_I11060/annotation1197095/range1197095/1
type: Range
persistent identity: range1197095
display id: range1197095
version: 1
direction: direction/forward
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start: 1125
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orientation: inline

spacer
type: SequenceAnnotation
persistent identity: annotation1197098
display id: annotation1197098
version: 1
top level: BBa_I11060
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I11060/annotation1197098/range1197098/1

https://synbiohub.org/public/igem/BBa_I11060/annotation1197098/range1197098/1
type: Range
persistent identity: range1197098
display id: range1197098
version: 1
direction: direction/forward
top level: BBa_I11060
start: 1140
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orientation: inline

AclI
type: SequenceAnnotation
persistent identity: annotation1197097
display id: annotation1197097
version: 1
top level: BBa_I11060
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I11060/annotation1197097/range1197097/1

https://synbiohub.org/public/igem/BBa_I11060/annotation1197097/range1197097/1
type: Range
persistent identity: range1197097
display id: range1197097
version: 1
direction: direction/forward
top level: BBa_I11060
start: 1134
end: 1139
orientation: inline

RBS (BBa_I11010)
type: SequenceAnnotation
persistent identity: annotation1197110
display id: annotation1197110
version: 1
top level: BBa_I11060
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_I11060/annotation1197110/range1197110/1

https://synbiohub.org/public/igem/BBa_I11060/annotation1197110/range1197110/1
type: Range
persistent identity: range1197110
display id: range1197110
version: 1
direction: direction/forward
top level: BBa_I11060
start: 1593
end: 1598
orientation: inline

spacer
type: SequenceAnnotation
persistent identity: annotation1197104
display id: annotation1197104
version: 1
top level: BBa_I11060
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I11060/annotation1197104/range1197104/1

https://synbiohub.org/public/igem/BBa_I11060/annotation1197104/range1197104/1
type: Range
persistent identity: range1197104
display id: range1197104
version: 1
direction: direction/forward
top level: BBa_I11060
start: 1545
end: 1547
orientation: inline

spacer
type: SequenceAnnotation
persistent identity: annotation1197108
display id: annotation1197108
version: 1
top level: BBa_I11060
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I11060/annotation1197108/range1197108/1

https://synbiohub.org/public/igem/BBa_I11060/annotation1197108/range1197108/1
type: Range
persistent identity: range1197108
display id: range1197108
version: 1
direction: direction/forward
top level: BBa_I11060
start: 1584
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orientation: inline

PvuI
type: SequenceAnnotation
persistent identity: annotation1197101
display id: annotation1197101
version: 1
top level: BBa_I11060
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I11060/annotation1197101/range1197101/1

https://synbiohub.org/public/igem/BBa_I11060/annotation1197101/range1197101/1
type: Range
persistent identity: range1197101
display id: range1197101
version: 1
direction: direction/forward
top level: BBa_I11060
start: 1406
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orientation: inline

spacer
type: SequenceAnnotation
persistent identity: annotation1197106
display id: annotation1197106
version: 1
top level: BBa_I11060
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I11060/annotation1197106/range1197106/1

https://synbiohub.org/public/igem/BBa_I11060/annotation1197106/range1197106/1
type: Range
persistent identity: range1197106
display id: range1197106
version: 1
direction: direction/forward
top level: BBa_I11060
start: 1554
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orientation: inline

BBa_I13509
type: ComponentDefinition
persistent identity: BBa I13509
display id: BBa_I13509
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Screening plasmid intermediate
creator: jkm
created: 2005-05-30T11:00:00Z
modified: 2015-08-31T04:07:34Z
discontinued: true
dominant: false
experience: experience/None
group u list: _11_
m user id: 0
owner id: 253
owning group id: 6
part status: Released HQ 2013
sample status: Discontinued
status: status/Deleted
bookmark: false
mutable description: Built by Josh as an intermediate in screening plasmid construction.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_I0500
component: BBa_E1010
component: BBa_B0032
sequence annotation: BBa_I0500
sequence annotation: BBa_E1010
sequence annotation: BBa_B0032
sequence: https://synbiohub.org/public/igem/BBa_I13509_sequence/1

BBa_I0500
type: Component
persistent identity: component1499292
display id: component1499292
version: 1
top level: BBa_I13509
access: public
definition: https://synbiohub.org/public/igem/BBa_I0500/1

BBa_E1010
type: Component
persistent identity: component1499308
display id: component1499308
version: 1
top level: BBa_I13509
access: public
definition: https://synbiohub.org/public/igem/BBa_E1010/1

BBa_B0032
type: Component
persistent identity: component1499300
display id: component1499300
version: 1
top level: BBa_I13509
access: public
definition: https://synbiohub.org/public/igem/BBa_B0032/1

BBa_I0500
type: SequenceAnnotation
persistent identity: annotation1499292
display id: annotation1499292
version: 1
top level: BBa_I13509
location: https://synbiohub.org/public/igem/BBa_I13509/annotation1499292/range1499292/1
component: BBa_I0500

https://synbiohub.org/public/igem/BBa_I13509/annotation1499292/range1499292/1
type: Range
persistent identity: range1499292
display id: range1499292
version: 1
direction: direction/forward
top level: BBa_I13509
start: 1
end: 1210
orientation: inline

BBa_E1010
type: SequenceAnnotation
persistent identity: annotation1499308
display id: annotation1499308
version: 1
top level: BBa_I13509
location: https://synbiohub.org/public/igem/BBa_I13509/annotation1499308/range1499308/1
component: BBa_E1010

https://synbiohub.org/public/igem/BBa_I13509/annotation1499308/range1499308/1
type: Range
persistent identity: range1499308
display id: range1499308
version: 1
direction: direction/forward
top level: BBa_I13509
start: 1238
end: 1918
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation1499300
display id: annotation1499300
version: 1
top level: BBa_I13509
location: https://synbiohub.org/public/igem/BBa_I13509/annotation1499300/range1499300/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_I13509/annotation1499300/range1499300/1
type: Range
persistent identity: range1499300
display id: range1499300
version: 1
direction: direction/forward
top level: BBa_I13509
start: 1219
end: 1231
orientation: inline

BBa_I13407
type: ComponentDefinition
persistent identity: BBa I13407
display id: BBa_I13407
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: YFP/CFP screening plasmid
creator: jasonk
created: 2005-05-01T11:00:00Z
modified: 2015-08-31T04:07:34Z
discontinued: false
dominant: false
experience: experience/None
group u list: _6_
m user id: 0
owner id: 2
owning group id: 6
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: A screening plasmid using YFP and CFP. The initial Pbad promoter has two extra restriction sites, HindIII and NcoI.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_I0500
component: BBa_E0030
component: BBa_E0020
component: BBa_B0012
component: BBa_I13406
component: BBa_B0010
component: BBa_B0034
sequence annotation: BBa_B0012
sequence annotation: BBa_B0034
sequence annotation: BBa_E0030
sequence annotation: BBa_B0010
sequence annotation: BBa_E0020
sequence annotation: BBa_I0500
sequence annotation: BBa_I13406
sequence: https://synbiohub.org/public/igem/BBa_I13407_sequence/1

BBa_I0500
type: Component
persistent identity: component2229909
display id: component2229909
version: 1
top level: BBa_I13407
access: public
definition: https://synbiohub.org/public/igem/BBa_I0500/1

BBa_E0030
type: Component
persistent identity: component2229899
display id: component2229899
version: 1
top level: BBa_I13407
access: public
definition: https://synbiohub.org/public/igem/BBa_E0030/1

BBa_E0020
type: Component
persistent identity: component2229912
display id: component2229912
version: 1
top level: BBa_I13407
access: public
definition: https://synbiohub.org/public/igem/BBa_E0020/1

BBa_B0012
type: Component
persistent identity: component2229902
display id: component2229902
version: 1
top level: BBa_I13407
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_I13406
type: Component
persistent identity: component2229896
display id: component2229896
version: 1
top level: BBa_I13407
access: public
definition: https://synbiohub.org/public/igem/BBa_I13406/1

BBa_B0010
type: Component
persistent identity: component2229900
display id: component2229900
version: 1
top level: BBa_I13407
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0034
type: Component
persistent identity: component2229898
display id: component2229898
version: 1
top level: BBa_I13407
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0012
type: Component
persistent identity: component2229915
display id: component2229915
version: 1
top level: BBa_I13407
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0034
type: Component
persistent identity: component2229911
display id: component2229911
version: 1
top level: BBa_I13407
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0010
type: Component
persistent identity: component2229913
display id: component2229913
version: 1
top level: BBa_I13407
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2229902
display id: annotation2229902
version: 1
top level: BBa_I13407
location: https://synbiohub.org/public/igem/BBa_I13407/annotation2229902/range2229902/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I13407/annotation2229902/range2229902/1
type: Range
persistent identity: range2229902
display id: range2229902
version: 1
direction: direction/forward
top level: BBa_I13407
start: 2072
end: 2112
orientation: inline

BBa_B0034
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BBa_B0010
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BBa_B0034
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BBa_I13406
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BBa_I0100
type: ComponentDefinition
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display id: BBa_I0100
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was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CAT Protein Generator
creator: cconboy
created: 2004-12-20T12:00:00Z
modified: 2015-08-31T04:07:28Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_1_
m user id: 0
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bookmark: false
mutable description: -- No description --
mutable provenance: Sauer lab expression vector
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owned by: myers
star: false
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BBa_I13711
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display id: BBa_I13711
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Tet promoter, Elowitz RBS, CheB
creator: Victoria Chou, Kenneth Nesmith, Madeleine Sheldon-Dante
created: 2004-08-12T11:00:00Z
modified: 2015-08-31T04:07:36Z
discontinued: false
dominant: false
experience: experience/None
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m user id: 0
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sample status: Not in stock
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bookmark: false
mutable description: -- No description --
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owned by: myers
star: false
type: DnaRegion
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component: BBa_B0034
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BBa_I14061
type: ComponentDefinition
persistent identity: BBa I14061
display id: BBa_I14061
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Constitutive aiiA & dsRed using P(Lac) IQ
creator: Vikram Vijayan, Allen Hsu, Lawrence Fomundam
created: 2004-08-04T11:00:00Z
modified: 2015-08-31T04:07:38Z
discontinued: false
dominant: false
experience: experience/None
group u list: _4_
m user id: 0
owner id: 171
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sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: p(Lac) IQ, Medium RBS, aiiA + LVA, Strong RBS, dsRed - LVA
owned by: james
owned by: myers
star: false
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BBa_I14030
type: ComponentDefinition
persistent identity: BBa I14030
display id: BBa_I14030
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Tet Toggle (strong RBS, weak RBS)
creator: Vikram Vijayan, Allen Hsu, Lawrence Fomundam
created: 2004-08-02T11:00:00Z
modified: 2015-08-31T04:07:37Z
discontinued: false
dominant: false
experience: experience/None
group u list: _4_
m user id: 0
owner id: 171
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
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owned by: james
owned by: myers
star: false
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component: BBa_R0040
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BBa_E0032
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BBa_C0051
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BBa_R0040
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BBa_C0012
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BBa_B0030
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BBa_C0051
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BBa_I14010
type: ComponentDefinition
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display id: BBa_I14010
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: araC with strong RBS
creator: Aditi Shrivastava, Jiwon Lee, Sarah Welch
created: 2004-07-27T11:00:00Z
modified: 2015-08-31T04:07:37Z
discontinued: false
dominant: true
experience: experience/None
group u list: _4_
m user id: 0
owner id: 166
owning group id: 7
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: BBa_B0030 BBa_C0080
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owned by: myers
star: false
type: DnaRegion
role: SO:0000804
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sequence annotation: BBa_B0030
sequence annotation: BBa_C0080
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BBa_C0080
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top level: BBa_I14010
access: public
definition: https://synbiohub.org/public/igem/BBa_C0080/1

BBa_B0030
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version: 1
top level: BBa_I14010
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definition: https://synbiohub.org/public/igem/BBa_B0030/1

BBa_B0030
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display id: BBa_Q01500
version: 1
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description: QPI (B0031.C0050.B0015.R0050)
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modified: 2015-05-08T01:14:13Z
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m user id: 0
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component: BBa_B0031
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persistent identity: BBa I13544
display id: BBa_I13544
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description: Intermediate in two promoter screening plasmid
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created: 2005-08-21T11:00:00Z
modified: 2015-08-31T04:07:35Z
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m user id: 0
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bookmark: false
mutable description: -- No description --
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owned by: myers
star: false
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persistent identity: BBa I13506
display id: BBa_I13506
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was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Screening plasmid intermediate
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modified: 2015-08-31T04:07:34Z
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experience: experience/None
group u list: _11_
m user id: 0
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sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Built by Josh as an intermediate in screening plasmid construction.
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owned by: myers
star: false
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BBa_J06107
type: ComponentDefinition
persistent identity: BBa J06107
display id: BBa_J06107
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was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: YFP and LasI(LVA+) on LasBox
creator: kxjin
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modified: 2015-08-31T04:08:16Z
discontinued: false
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group u list: _20_
m user id: 0
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status: status/Unavailable
bookmark: false
mutable description: -- No description --
owned by: james
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star: false
type: DnaRegion
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type: ComponentDefinition
persistent identity: BBa Q01511
display id: BBa_Q01511
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: QPI (B0031.C0051.B0015.R0065)
creator: Caitlin Conboy and Jennifer Braff
created: 2003-12-02T12:00:00Z
modified: 2015-05-08T01:14:13Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
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part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: true
type: DnaRegion
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description: 434 cI QPI regulated by Lambda cI with mCherry
creator: ytwang
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description: CheR behind a tet promoter and Elowitz RBS
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created: 2004-08-12T11:00:00Z
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description: C4, LacI Router (medium RBS)
creator: Vikram Vijayan, Allen Hsu, Lawrence Fomundam
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modified: 2015-08-31T04:07:38Z
discontinued: false
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experience: experience/None
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bookmark: false
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owned by: myers
star: false
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description: 434 cI regulatable expression of YFP
creator: Olmecs 2003 IAP Team
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modified: 2015-08-31T04:07:42Z
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experience: experience/Issues
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status: status/Planning
bookmark: false
mutable description: -- No description --
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star: false
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version: 1
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BBa_I15004
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display id: BBa_I15004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LuxR generator + LacI regulated lambda cI (LVA)+LuxI (LVA)
creator: Jeff Tabor
created: 2004-05-25T11:00:00Z
modified: 2015-08-31T04:07:38Z
discontinued: false
dominant: false
experience: experience/None
group u list: _5_
m user id: 0
owner id: 88
owning group id: 7
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status: status/Planning
bookmark: false
mutable description: Polycistronic lambda cI and LuxI proteins regulated by Lac Repressor (LacI). Downstream is consitutive LuxR expression cassette. System flanked by dual transcription terminators. System incompatible with TetR.
mutable provenance: formerly I15001
owned by: james
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star: false
type: DnaRegion
role: SO:0000804
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component: BBa_C0051
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component: BBa_R0040
component: BBa_B0015
component: BBa_F1610
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sequence annotation: BBa_I0462
sequence annotation: BBa_C0051
sequence annotation: BBa_F1610
sequence annotation: BBa_B0015
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sequence annotation: BBa_B0034
sequence annotation: BBa_R0040
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display id: component2234039
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persistent identity: component2234057
display id: component2234057
version: 1
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access: public
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BBa_F1610
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BBa_I0462
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end: 993
orientation: inline

BBa_F1610
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BBa_B0015
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direction: direction/reverse
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BBa_R0011
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BBa_B0034
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BBa_R0040
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BBa_I13539
type: ComponentDefinition
persistent identity: BBa I13539
display id: BBa_I13539
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Two promoter screening plasmid v1.2
creator: Josh Michener
created: 2005-08-21T11:00:00Z
modified: 2015-08-31T04:07:35Z
discontinued: false
dominant: false
group u list: _11_
m user id: 0
owner id: 253
owning group id: 6
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_E1010
component: BBa_I13457
component: BBa_B0010
component: BBa_E0040
component: BBa_B0034
component: BBa_B0012
component: BBa_I0500
component: BBa_I13453
sequence annotation: BBa_B0034
sequence annotation: BBa_I13457
sequence annotation: BBa_B0012
sequence annotation: BBa_I0500
sequence annotation: BBa_B0010
sequence annotation: BBa_E1010
sequence annotation: BBa_I13453
sequence annotation: BBa_E0040
sequence: https://synbiohub.org/public/igem/BBa_I13539_sequence/1

BBa_E1010
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display id: component1679611
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BBa_I13457
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BBa_B0010
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definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_E0040
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display id: component1679639
version: 1
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access: public
definition: https://synbiohub.org/public/igem/BBa_E0040/1

BBa_B0034
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BBa_B0034
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BBa_B0010
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BBa_B0012
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direction: direction/forward
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BBa_B0012
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persistent identity: annotation1679644
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type: SequenceAnnotation
persistent identity: annotation1679637
display id: annotation1679637
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persistent identity: range1679637
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direction: direction/forward
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BBa_P0477
type: ComponentDefinition
persistent identity: BBa P0477
display id: BBa_P0477
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: B0034.C0077.B0015
creator: cconboy
created: 2004-07-27T11:00:00Z
modified: 2015-05-08T01:14:11Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_1_
m user id: 0
owner id: 60
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0012
component: BBa_C0077
component: BBa_B0010
component: BBa_B0034
sequence annotation: BBa_B0012
sequence annotation: BBa_C0077
sequence annotation: BBa_B0034
sequence annotation: BBa_B0010
sequence: https://synbiohub.org/public/igem/BBa_P0477_sequence/1

BBa_B0012
type: Component
persistent identity: component1014823
display id: component1014823
version: 1
top level: BBa_P0477
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_C0077
type: Component
persistent identity: component1014807
display id: component1014807
version: 1
top level: BBa_P0477
access: public
definition: https://synbiohub.org/public/igem/BBa_C0077/1

BBa_B0010
type: Component
persistent identity: component1014813
display id: component1014813
version: 1
top level: BBa_P0477
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0034
type: Component
persistent identity: component1014797
display id: component1014797
version: 1
top level: BBa_P0477
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0012
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persistent identity: annotation1014823
display id: annotation1014823
version: 1
top level: BBa_P0477
location: https://synbiohub.org/public/igem/BBa_P0477/annotation1014823/range1014823/1
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persistent identity: range1014823
display id: range1014823
version: 1
direction: direction/forward
top level: BBa_P0477
start: 902
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orientation: inline

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type: SequenceAnnotation
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display id: annotation1014807
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display id: range1014807
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direction: direction/forward
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orientation: inline

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type: SequenceAnnotation
persistent identity: annotation1014797
display id: annotation1014797
version: 1
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persistent identity: range1014797
display id: range1014797
version: 1
direction: direction/forward
top level: BBa_P0477
start: 1
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orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1014813
display id: annotation1014813
version: 1
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persistent identity: range1014813
display id: range1014813
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direction: direction/forward
top level: BBa_P0477
start: 814
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orientation: inline

BBa_I14045
type: ComponentDefinition
persistent identity: BBa I14045
display id: BBa_I14045
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: strong RBS . RhlR
creator: Aditi Shrivastava, Jiwon Lee, Sarah Welch
created: 2004-08-03T11:00:00Z
modified: 2015-08-31T04:07:38Z
discontinued: false
dominant: false
experience: experience/None
group u list: _4_
m user id: 0
owner id: 166
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: BBa_B0030 BBa_C0071
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0030
component: BBa_C0071
sequence annotation: BBa_C0071
sequence annotation: BBa_B0030
sequence: https://synbiohub.org/public/igem/BBa_I14045_sequence/1

BBa_B0030
type: Component
persistent identity: component1024813
display id: component1024813
version: 1
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access: public
definition: https://synbiohub.org/public/igem/BBa_B0030/1

BBa_C0071
type: Component
persistent identity: component1024824
display id: component1024824
version: 1
top level: BBa_I14045
access: public
definition: https://synbiohub.org/public/igem/BBa_C0071/1

BBa_C0071
type: SequenceAnnotation
persistent identity: annotation1024824
display id: annotation1024824
version: 1
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orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation1024813
display id: annotation1024813
version: 1
top level: BBa_I14045
location: https://synbiohub.org/public/igem/BBa_I14045/annotation1024813/range1024813/1
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persistent identity: range1024813
display id: range1024813
version: 1
direction: direction/forward
top level: BBa_I14045
start: 1
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orientation: inline

BBa_I13516
type: ComponentDefinition
persistent identity: BBa I13516
display id: BBa_I13516
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Screening Plasmid Intermediate
creator: jkm
created: 2005-06-05T11:00:00Z
modified: 2015-08-31T04:07:34Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_
m user id: 0
owner id: 253
owning group id: 6
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Built by Josh as an intermediate in screening plasmid construction.
mutable notes: Deletion of the C at position 1194. This is right at the end of I0500, after the transcriptional start of the downstream gene but before the RBS. Shouldn't make a difference, but needs to be checked out.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_I0500
component: BBa_B0034
component: BBa_E1010
sequence annotation: BBa_E1010
sequence annotation: BBa_I0500
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_I13516_sequence/1

BBa_I0500
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persistent identity: component1505485
display id: component1505485
version: 1
top level: BBa_I13516
access: public
definition: https://synbiohub.org/public/igem/BBa_I0500/1

BBa_B0034
type: Component
persistent identity: component1505490
display id: component1505490
version: 1
top level: BBa_I13516
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

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description: R0011.I13277 Lac-Regulated Cin Amplifier
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created: 2004-08-08T11:00:00Z
modified: 2015-08-31T04:07:33Z
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experience: experience/None
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bookmark: false
mutable description: -- No description --
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description: R0010.E0021.B0024.E0031
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display id: BBa_I13542
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Intermediate in two promoter screening plasmid
creator: Josh Michener
created: 2005-08-21T11:00:00Z
modified: 2015-08-31T04:07:35Z
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dominant: false
group u list: _11_
m user id: 0
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bookmark: false
mutable description: -- No description --
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star: false
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type: ComponentDefinition
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display id: BBa_I13518
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Screening Plasmid Intermediate
creator: jkm
created: 2005-06-05T11:00:00Z
modified: 2015-08-31T04:07:34Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_
m user id: 0
owner id: 253
owning group id: 6
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
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owned by: james
owned by: myers
star: false
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role: SO:0000804
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BBa_I5110
type: ComponentDefinition
persistent identity: BBa I5110
display id: BBa_I5110
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Lambda cI regulatable expression of YFP
creator: Olmecs 2003 IAP Team
created: 2003-11-27T12:00:00Z
modified: 2015-08-31T04:07:42Z
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
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owning group id: 7
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: -- No description --
owned by: james
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star: false
type: DnaRegion
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BBa_R0051
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BBa_E0032
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BBa_B0012
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BBa_E0032
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BBa_B0012
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BBa_B0011
type: SequenceAnnotation
persistent identity: annotation948017
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BBa_I14003
type: ComponentDefinition
persistent identity: BBa I14003
display id: BBa_I14003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TetR with strong RBS
creator: Aditi Shrivastava, Jiwon Lee, Sarah Welch
created: 2004-07-25T11:00:00Z
modified: 2015-08-31T04:07:37Z
discontinued: false
dominant: false
experience: experience/None
group u list: _4_
m user id: 0
owner id: 166
owning group id: 7
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: B0030 C0040
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0030
component: BBa_C0040
sequence annotation: BBa_B0030
sequence annotation: BBa_C0040
sequence: https://synbiohub.org/public/igem/BBa_I14003_sequence/1

BBa_B0030
type: Component
persistent identity: component1018172
display id: component1018172
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access: public
definition: https://synbiohub.org/public/igem/BBa_B0030/1

BBa_C0040
type: Component
persistent identity: component1018183
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version: 1
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access: public
definition: https://synbiohub.org/public/igem/BBa_C0040/1

BBa_B0030
type: SequenceAnnotation
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BBa_C0040
type: SequenceAnnotation
persistent identity: annotation1018183
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version: 1
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component: BBa_C0040

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BBa_I0401
type: ComponentDefinition
persistent identity: BBa I0401
display id: BBa_I0401
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was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: R0040.B0034.C0062.E0420
creator: jcbraff
created: 2004-06-08T11:00:00Z
modified: 2015-08-31T04:07:28Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_1_
m user id: 0
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sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: R0040.B0034.C0062.E0420
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_R0040
component: BBa_B0034
component: BBa_B0012
component: BBa_B0010
component: BBa_C0062
component: BBa_E0020
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sequence annotation: BBa_E0020
sequence annotation: BBa_B0010
sequence annotation: BBa_R0040
sequence annotation: BBa_B0012
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BBa_R0040
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BBa_B0034
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BBa_B0012
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definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0010
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definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0034
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BBa_C0062
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BBa_E0020
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BBa_C0062
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BBa_E0020
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BBa_B0010
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BBa_R0040
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BBa_B0012
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BBa_B0034
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BBa_B0034
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component: BBa_B0034

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display id: range958416
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BBa_E0433
type: ComponentDefinition
persistent identity: BBa E0433
display id: BBa_E0433
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: B0034.E0033.B0015
creator: cconboy
created: 2004-05-24T11:00:00Z
modified: 2015-08-31T04:07:26Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_1_
m user id: 0
owner id: 60
owning group id: 7
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Reporter containing LacZ alpha fragment. Requires complementation with omega fragment. (See notes on E0033)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_E0033
component: BBa_B0034
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sequence: https://synbiohub.org/public/igem/BBa_E0433_sequence/1

BBa_E0033
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definition: https://synbiohub.org/public/igem/BBa_E0033/1

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definition: https://synbiohub.org/public/igem/BBa_B0034/1

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display id: component2220188
version: 1
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definition: https://synbiohub.org/public/igem/BBa_B0015/1

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display id: range2220181
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orientation: inline

BBa_Q01510
type: ComponentDefinition
persistent identity: BBa Q01510
display id: BBa_Q01510
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: QPI (B0031.C0051.B0015.R0051)
creator: Caitlin Conboy and Jennifer Braff
created: 2003-12-02T12:00:00Z
modified: 2015-05-08T01:14:13Z
discontinued: false
dominant: true
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_C0051
component: BBa_R0051
component: BBa_B0012
component: BBa_B0010
component: BBa_B0031
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sequence annotation: BBa_B0031
sequence annotation: BBa_C0051
sequence annotation: BBa_B0012
sequence annotation: BBa_B0010
sequence: https://synbiohub.org/public/igem/BBa_Q01510_sequence/1

BBa_C0051
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version: 1
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access: public
definition: https://synbiohub.org/public/igem/BBa_C0051/1

BBa_R0051
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definition: https://synbiohub.org/public/igem/BBa_R0051/1

BBa_B0012
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definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0010
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display id: component951882
version: 1
top level: BBa_Q01510
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definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0031
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display id: component951866
version: 1
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access: public
definition: https://synbiohub.org/public/igem/BBa_B0031/1

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orientation: inline

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orientation: inline

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version: 1
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persistent identity: range951892
display id: range951892
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direction: direction/forward
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end: 932
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation951882
display id: annotation951882
version: 1
top level: BBa_Q01510
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component: BBa_B0010

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persistent identity: range951882
display id: range951882
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BBa_Q03122
type: ComponentDefinition
persistent identity: BBa Q03122
display id: BBa_Q03122
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: QPI (B0033.C0012.B0015.R0010)
creator: Caitlin Conboy and Jennifer Braff
created: 2003-12-02T12:00:00Z
modified: 2015-05-08T01:14:13Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0033
component: BBa_B0012
component: BBa_C0012
component: BBa_R0010
component: BBa_B0010
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sequence annotation: BBa_R0010
sequence annotation: BBa_B0033
sequence annotation: BBa_B0010
sequence annotation: BBa_C0012
sequence: https://synbiohub.org/public/igem/BBa_Q03122_sequence/1

BBa_B0033
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display id: component952237
version: 1
top level: BBa_Q03122
access: public
definition: https://synbiohub.org/public/igem/BBa_B0033/1

BBa_B0012
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display id: component952265
version: 1
top level: BBa_Q03122
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_C0012
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display id: component952248
version: 1
top level: BBa_Q03122
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definition: https://synbiohub.org/public/igem/BBa_C0012/1

BBa_R0010
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display id: component952281
version: 1
top level: BBa_Q03122
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definition: https://synbiohub.org/public/igem/BBa_R0010/1

BBa_B0010
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definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0012
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BBa_R0010
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version: 1
top level: BBa_Q03122
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description: Constitutive RhlR
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modified: 2015-08-31T04:07:37Z
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dominant: false
experience: experience/None
group u list: _4_
m user id: 0
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status: status/Unavailable
bookmark: false
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owned by: james
owned by: myers
star: false
type: DnaRegion
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description: Two promoter screening plasmid v1.1
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modified: 2015-08-31T04:07:35Z
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dominant: false
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m user id: 0
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status: status/Available
bookmark: false
mutable description: -- No description --
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owned by: myers
star: false
type: DnaRegion
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BBa_I13519
type: ComponentDefinition
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display id: BBa_I13519
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Screening Plasmid Intermediate
creator: jkm
created: 2005-06-05T11:00:00Z
modified: 2015-08-31T04:07:34Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_
m user id: 0
owner id: 253
owning group id: 6
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Built by Josh as an intermediate in screening plasmid construction.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
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BBa_I13452
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BBa_B0010
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BBa_E5501
type: ComponentDefinition
persistent identity: BBa E5501
display id: BBa_E5501
version: 1
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description: B0032.E0040
creator: jcbraff
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modified: 2015-08-31T04:07:27Z
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experience: experience/None
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bookmark: true
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owned by: myers
star: false
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BBa_B0032
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definition: https://synbiohub.org/public/igem/BBa_B0032/1

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BBa_I14036
type: ComponentDefinition
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display id: BBa_I14036
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: C12, CI Router (medium RBS)
creator: Vikram Vijayan, Allen Hsu, Lawrence Fomundam
created: 2004-08-03T11:00:00Z
modified: 2015-08-31T04:07:38Z
discontinued: false
dominant: false
experience: experience/None
group u list: _4_
m user id: 0
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sample status: Not in stock
status: status/Unavailable
bookmark: false
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owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
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BBa_R0079
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description: I13308.E0430 Tet-Regulated Cin Amplifier with YFP Reporter
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mutable description: -- No description --
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tetR
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was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: AHL receiver, CI repressor hybrid
creator: sash31
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modified: 2015-08-31T04:08:16Z
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experience: experience/None
group u list: _20_
m user id: 0
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status: status/Unavailable
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owned by: myers
star: false
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component: BBa_R0062
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BBa_Q04811
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description: QPI (B0034.C0081.B0015.R0051)
creator: Alvin Carter Powers, Yong-Su Jin (Fighting Darwins)
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modified: 2015-05-08T01:14:14Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
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sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: RBS, cI-857 coding region, terminator, cI-857 binding region repressor inverter used in heat trigger
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star: false
type: DnaRegion
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BBa_B0010
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BBa_B0034
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BBa_B0012
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BBa_R0051
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BBa_C0081
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component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I13310/annotation1037222/range1037222/1
type: Range
persistent identity: range1037222
display id: range1037222
version: 1
direction: direction/forward
top level: BBa_I13310
start: 1016
end: 1027
orientation: inline

BBa_I5611
type: ComponentDefinition
persistent identity: BBa I5611
display id: BBa_I5611
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Brickalator (LVA-)
creator: Caitlin Conboy and Jennifer Braff
created: 2004-04-20T11:00:00Z
modified: 2015-08-31T04:07:42Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: QPI-optimized Elowitz Repressilator. YFP output device w/o ssra tail. (R0040.Q04510.Q04121.Q04400.E0430)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_Q04510
component: BBa_E0430
component: BBa_R0040
component: BBa_Q04400
component: BBa_Q04121
sequence annotation: BBa_Q04121
sequence annotation: BBa_R0040
sequence annotation: BBa_E0430
sequence annotation: BBa_Q04510
sequence annotation: BBa_Q04400
sequence: https://synbiohub.org/public/igem/BBa_I5611_sequence/1

BBa_Q04510
type: Component
persistent identity: component2237547
display id: component2237547
version: 1
top level: BBa_I5611
access: public
definition: https://synbiohub.org/public/igem/BBa_Q04510/1

BBa_E0430
type: Component
persistent identity: component2237594
display id: component2237594
version: 1
top level: BBa_I5611
access: public
definition: https://synbiohub.org/public/igem/BBa_E0430/1

BBa_R0040
type: Component
persistent identity: component2237525
display id: component2237525
version: 1
top level: BBa_I5611
access: public
definition: https://synbiohub.org/public/igem/BBa_R0040/1

BBa_Q04400
type: Component
persistent identity: component2237584
display id: component2237584
version: 1
top level: BBa_I5611
access: public
definition: https://synbiohub.org/public/igem/BBa_Q04400/1

BBa_Q04121
type: Component
persistent identity: component2237566
display id: component2237566
version: 1
top level: BBa_I5611
access: public
definition: https://synbiohub.org/public/igem/BBa_Q04121/1

BBa_Q04121
type: SequenceAnnotation
persistent identity: annotation2237566
display id: annotation2237566
version: 1
top level: BBa_I5611
location: https://synbiohub.org/public/igem/BBa_I5611/annotation2237566/range2237566/1
component: BBa_Q04121

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type: Range
persistent identity: range2237566
display id: range2237566
version: 1
direction: direction/reverse
top level: BBa_I5611
start: 1058
end: 2428
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation2237525
display id: annotation2237525
version: 1
top level: BBa_I5611
location: https://synbiohub.org/public/igem/BBa_I5611/annotation2237525/range2237525/1
component: BBa_R0040

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type: Range
persistent identity: range2237525
display id: range2237525
version: 1
direction: direction/forward
top level: BBa_I5611
start: 1
end: 54
orientation: inline

BBa_E0430
type: SequenceAnnotation
persistent identity: annotation2237594
display id: annotation2237594
version: 1
top level: BBa_I5611
location: https://synbiohub.org/public/igem/BBa_I5611/annotation2237594/range2237594/1
component: BBa_E0430

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type: Range
persistent identity: range2237594
display id: range2237594
version: 1
direction: direction/reverse
top level: BBa_I5611
start: 3347
end: 4224
orientation: inline

BBa_Q04510
type: SequenceAnnotation
persistent identity: annotation2237547
display id: annotation2237547
version: 1
top level: BBa_I5611
location: https://synbiohub.org/public/igem/BBa_I5611/annotation2237547/range2237547/1
component: BBa_Q04510

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type: Range
persistent identity: range2237547
display id: range2237547
version: 1
direction: direction/reverse
top level: BBa_I5611
start: 63
end: 1049
orientation: inline

BBa_Q04400
type: SequenceAnnotation
persistent identity: annotation2237584
display id: annotation2237584
version: 1
top level: BBa_I5611
location: https://synbiohub.org/public/igem/BBa_I5611/annotation2237584/range2237584/1
component: BBa_Q04400

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type: Range
persistent identity: range2237584
display id: range2237584
version: 1
direction: direction/reverse
top level: BBa_I5611
start: 2437
end: 3338
orientation: inline

BBa_I5121
type: ComponentDefinition
persistent identity: BBa I5121
display id: BBa_I5121
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: 434 cI regulatable expression of YFP (LVA-)
creator: Reshma Shetty
created: 2005-05-12T11:00:00Z
modified: 2015-08-31T04:07:42Z
discontinued: false
dominant: false
experience: experience/Issues
group u list: _41_44_48_46_1_
m user id: 0
owner id: 126
owning group id: 6
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Identical to BBa_I5120 except the LVA tag has been removed.
mutable notes: Identical to BBa_I5120 except the LVA tag has been removed. It was removed using a hack in which I simply amplified the everything in the plasmid except the tag and did a blunt end ligation.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_E0030
component: BBa_R0052
component: BBa_B0034
component: BBa_B0011
component: BBa_B0012
sequence annotation: BBa_B0034
sequence annotation: BBa_E0030
sequence annotation: BBa_B0011
sequence annotation: BBa_R0052
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_I5121_sequence/1

BBa_E0030
type: Component
persistent identity: component1487619
display id: component1487619
version: 1
top level: BBa_I5121
access: public
definition: https://synbiohub.org/public/igem/BBa_E0030/1

BBa_R0052
type: Component
persistent identity: component1487607
display id: component1487607
version: 1
top level: BBa_I5121
access: public
definition: https://synbiohub.org/public/igem/BBa_R0052/1

BBa_B0034
type: Component
persistent identity: component1487617
display id: component1487617
version: 1
top level: BBa_I5121
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0011
type: Component
persistent identity: component1487636
display id: component1487636
version: 1
top level: BBa_I5121
access: public
definition: https://synbiohub.org/public/igem/BBa_B0011/1

BBa_B0012
type: Component
persistent identity: component1487628
display id: component1487628
version: 1
top level: BBa_I5121
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1487617
display id: annotation1487617
version: 1
top level: BBa_I5121
location: https://synbiohub.org/public/igem/BBa_I5121/annotation1487617/range1487617/1
component: BBa_B0034

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type: Range
persistent identity: range1487617
display id: range1487617
version: 1
direction: direction/forward
top level: BBa_I5121
start: 55
end: 66
orientation: inline

BBa_E0030
type: SequenceAnnotation
persistent identity: annotation1487619
display id: annotation1487619
version: 1
top level: BBa_I5121
location: https://synbiohub.org/public/igem/BBa_I5121/annotation1487619/range1487619/1
component: BBa_E0030

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type: Range
persistent identity: range1487619
display id: range1487619
version: 1
direction: direction/forward
top level: BBa_I5121
start: 73
end: 795
orientation: inline

BBa_B0011
type: SequenceAnnotation
persistent identity: annotation1487636
display id: annotation1487636
version: 1
top level: BBa_I5121
location: https://synbiohub.org/public/igem/BBa_I5121/annotation1487636/range1487636/1
component: BBa_B0011

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type: Range
persistent identity: range1487636
display id: range1487636
version: 1
direction: direction/forward
top level: BBa_I5121
start: 853
end: 898
orientation: inline

BBa_R0052
type: SequenceAnnotation
persistent identity: annotation1487607
display id: annotation1487607
version: 1
top level: BBa_I5121
location: https://synbiohub.org/public/igem/BBa_I5121/annotation1487607/range1487607/1
component: BBa_R0052

https://synbiohub.org/public/igem/BBa_I5121/annotation1487607/range1487607/1
type: Range
persistent identity: range1487607
display id: range1487607
version: 1
direction: direction/forward
top level: BBa_I5121
start: 1
end: 46
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1487628
display id: annotation1487628
version: 1
top level: BBa_I5121
location: https://synbiohub.org/public/igem/BBa_I5121/annotation1487628/range1487628/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I5121/annotation1487628/range1487628/1
type: Range
persistent identity: range1487628
display id: range1487628
version: 1
direction: direction/forward
top level: BBa_I5121
start: 804
end: 844
orientation: inline

BBa_I15024
type: ComponentDefinition
persistent identity: BBa I15024
display id: BBa_I15024
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Tet-repressible polycistronic CFP/YFP under B0034
creator: Jeff Tabor
created: 2005-06-13T11:00:00Z
modified: 2015-08-31T04:07:39Z
discontinued: true
dominant: false
experience: experience/None
group u list: _5_
m user id: 0
owner id: 88
owning group id: 37
sample status: Discontinued
status: status/Deleted
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0034
component: BBa_R0040
component: BBa_E0030
component: BBa_B0012
component: BBa_E0020
component: BBa_B0010
sequence annotation: BBa_E0030
sequence annotation: BBa_B0010
sequence annotation: BBa_B0034
sequence annotation: BBa_E0020
sequence annotation: BBa_R0040
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_I15024_sequence/1

BBa_B0034
type: Component
persistent identity: component1516847
display id: component1516847
version: 1
top level: BBa_I15024
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_R0040
type: Component
persistent identity: component1516839
display id: component1516839
version: 1
top level: BBa_I15024
access: public
definition: https://synbiohub.org/public/igem/BBa_R0040/1

BBa_E0030
type: Component
persistent identity: component1516856
display id: component1516856
version: 1
top level: BBa_I15024
access: public
definition: https://synbiohub.org/public/igem/BBa_E0030/1

BBa_B0012
type: Component
persistent identity: component1516871
display id: component1516871
version: 1
top level: BBa_I15024
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_E0020
type: Component
persistent identity: component1516849
display id: component1516849
version: 1
top level: BBa_I15024
access: public
definition: https://synbiohub.org/public/igem/BBa_E0020/1

BBa_B0034
type: Component
persistent identity: component1516854
display id: component1516854
version: 1
top level: BBa_I15024
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0010
type: Component
persistent identity: component1516861
display id: component1516861
version: 1
top level: BBa_I15024
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_E0030
type: SequenceAnnotation
persistent identity: annotation1516856
display id: annotation1516856
version: 1
top level: BBa_I15024
location: https://synbiohub.org/public/igem/BBa_I15024/annotation1516856/range1516856/1
component: BBa_E0030

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type: Range
persistent identity: range1516856
display id: range1516856
version: 1
direction: direction/forward
top level: BBa_I15024
start: 830
end: 1552
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1516861
display id: annotation1516861
version: 1
top level: BBa_I15024
location: https://synbiohub.org/public/igem/BBa_I15024/annotation1516861/range1516861/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I15024/annotation1516861/range1516861/1
type: Range
persistent identity: range1516861
display id: range1516861
version: 1
direction: direction/forward
top level: BBa_I15024
start: 1561
end: 1640
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1516847
display id: annotation1516847
version: 1
top level: BBa_I15024
location: https://synbiohub.org/public/igem/BBa_I15024/annotation1516847/range1516847/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I15024/annotation1516847/range1516847/1
type: Range
persistent identity: range1516847
display id: range1516847
version: 1
direction: direction/forward
top level: BBa_I15024
start: 63
end: 74
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1516854
display id: annotation1516854
version: 1
top level: BBa_I15024
location: https://synbiohub.org/public/igem/BBa_I15024/annotation1516854/range1516854/1
component: BBa_B0034

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type: Range
persistent identity: range1516854
display id: range1516854
version: 1
direction: direction/forward
top level: BBa_I15024
start: 812
end: 823
orientation: inline

BBa_E0020
type: SequenceAnnotation
persistent identity: annotation1516849
display id: annotation1516849
version: 1
top level: BBa_I15024
location: https://synbiohub.org/public/igem/BBa_I15024/annotation1516849/range1516849/1
component: BBa_E0020

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type: Range
persistent identity: range1516849
display id: range1516849
version: 1
direction: direction/forward
top level: BBa_I15024
start: 81
end: 803
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1516839
display id: annotation1516839
version: 1
top level: BBa_I15024
location: https://synbiohub.org/public/igem/BBa_I15024/annotation1516839/range1516839/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_I15024/annotation1516839/range1516839/1
type: Range
persistent identity: range1516839
display id: range1516839
version: 1
direction: direction/forward
top level: BBa_I15024
start: 1
end: 54
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1516871
display id: annotation1516871
version: 1
top level: BBa_I15024
location: https://synbiohub.org/public/igem/BBa_I15024/annotation1516871/range1516871/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I15024/annotation1516871/range1516871/1
type: Range
persistent identity: range1516871
display id: range1516871
version: 1
direction: direction/forward
top level: BBa_I15024
start: 1649
end: 1689
orientation: inline

BBa_I14046
type: ComponentDefinition
persistent identity: BBa I14046
display id: BBa_I14046
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: strong RBS . RhlI
creator: JAS
created: 2004-08-03T11:00:00Z
modified: 2015-08-31T04:07:38Z
discontinued: false
dominant: true
experience: experience/None
group u list: _4_
m user id: 0
owner id: 166
owning group id: 7
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: BBa_B0030 BBa_C0070
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_C0070
component: BBa_B0030
sequence annotation: BBa_B0030
sequence annotation: BBa_C0070
sequence: https://synbiohub.org/public/igem/BBa_I14046_sequence/1

BBa_C0070
type: Component
persistent identity: component1024852
display id: component1024852
version: 1
top level: BBa_I14046
access: public
definition: https://synbiohub.org/public/igem/BBa_C0070/1

BBa_B0030
type: Component
persistent identity: component1024841
display id: component1024841
version: 1
top level: BBa_I14046
access: public
definition: https://synbiohub.org/public/igem/BBa_B0030/1

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation1024841
display id: annotation1024841
version: 1
top level: BBa_I14046
location: https://synbiohub.org/public/igem/BBa_I14046/annotation1024841/range1024841/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_I14046/annotation1024841/range1024841/1
type: Range
persistent identity: range1024841
display id: range1024841
version: 1
direction: direction/forward
top level: BBa_I14046
start: 1
end: 15
orientation: inline

BBa_C0070
type: SequenceAnnotation
persistent identity: annotation1024852
display id: annotation1024852
version: 1
top level: BBa_I14046
location: https://synbiohub.org/public/igem/BBa_I14046/annotation1024852/range1024852/1
component: BBa_C0070

https://synbiohub.org/public/igem/BBa_I14046/annotation1024852/range1024852/1
type: Range
persistent identity: range1024852
display id: range1024852
version: 1
direction: direction/forward
top level: BBa_I14046
start: 22
end: 663
orientation: inline

BBa_I14012
type: ComponentDefinition
persistent identity: BBa I14012
display id: BBa_I14012
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: B30, C79
creator: Vikram Vijayan, Allen Hsu, Lawrence Fomundam
created: 2004-07-29T11:00:00Z
modified: 2015-08-31T04:07:37Z
discontinued: false
dominant: false
experience: experience/None
group u list: _4_
m user id: 0
owner id: 171
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Strong RBS (B30), LasR + LVA (C79) LINKS OK
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0030
component: BBa_C0079
sequence annotation: BBa_C0079
sequence annotation: BBa_B0030
sequence: https://synbiohub.org/public/igem/BBa_I14012_sequence/1

BBa_B0030
type: Component
persistent identity: component1020050
display id: component1020050
version: 1
top level: BBa_I14012
access: public
definition: https://synbiohub.org/public/igem/BBa_B0030/1

BBa_C0079
type: Component
persistent identity: component1020064
display id: component1020064
version: 1
top level: BBa_I14012
access: public
definition: https://synbiohub.org/public/igem/BBa_C0079/1

BBa_C0079
type: SequenceAnnotation
persistent identity: annotation1020064
display id: annotation1020064
version: 1
top level: BBa_I14012
location: https://synbiohub.org/public/igem/BBa_I14012/annotation1020064/range1020064/1
component: BBa_C0079

https://synbiohub.org/public/igem/BBa_I14012/annotation1020064/range1020064/1
type: Range
persistent identity: range1020064
display id: range1020064
version: 1
direction: direction/forward
top level: BBa_I14012
start: 22
end: 777
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation1020050
display id: annotation1020050
version: 1
top level: BBa_I14012
location: https://synbiohub.org/public/igem/BBa_I14012/annotation1020050/range1020050/1
component: BBa_B0030

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type: Range
persistent identity: range1020050
display id: range1020050
version: 1
direction: direction/forward
top level: BBa_I14012
start: 1
end: 15
orientation: inline

BBa_I5410
type: ComponentDefinition
persistent identity: BBa I5410
display id: BBa_I5410
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Olmecs: Oscillator 2 of Synchro-Dualator
creator: Olmecs 2003 IAP Team
created: 2003-11-27T12:00:00Z
modified: 2015-08-31T04:07:42Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: For reference only
status: status/Informational
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_R0011
component: BBa_C0012
component: BBa_B0012
component: BBa_B0011
component: BBa_C0052
component: BBa_B0034
component: BBa_C0053
component: BBa_R0052
component: BBa_R0053
sequence annotation: BBa_B0034
sequence annotation: BBa_B0011
sequence annotation: BBa_R0053
sequence annotation: BBa_B0012
sequence annotation: BBa_C0053
sequence annotation: BBa_C0012
sequence annotation: BBa_C0052
sequence annotation: BBa_R0052
sequence annotation: BBa_R0011
sequence: https://synbiohub.org/public/igem/BBa_I5410_sequence/1

BBa_R0011
type: Component
persistent identity: component2234487
display id: component2234487
version: 1
top level: BBa_I5410
access: public
definition: https://synbiohub.org/public/igem/BBa_R0011/1

BBa_C0012
type: Component
persistent identity: component2234535
display id: component2234535
version: 1
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pen LacI
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was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: p(pen) . strong RBS . LacI
creator: Aditi Shrivastava, Jiwon Lee, Sarah Welch
created: 2004-07-25T11:00:00Z
modified: 2015-08-31T04:07:37Z
discontinued: true
dominant: false
experience: experience/None
group u list: _4_
m user id: 0
owner id: 166
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sample status: Discontinued
status: status/Deleted
bookmark: false
mutable description: BBa_R0074 BBa_I14002
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description: R0040.B0034.C0077.E0420
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modified: 2015-08-31T04:07:28Z
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dominant: false
experience: experience/None
group u list: _11_1_
m user id: 0
owner id: 61
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part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
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display id: BBa_M30109
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Light responsive system, dual regulation
creator: natalie kuldell
created: 2005-11-14T12:00:00Z
modified: 2015-05-08T01:13:59Z
discontinued: false
dominant: true
experience: experience/None
group u list: _45_1_
m user id: 0
owner id: 314
owning group id: 1
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Pbad regulated phycobilins (ho1 and pcyA) followed by Ptet regulated light sensor (Cph8). This should allow repressed Pbad to give low levels of accessory proteins and derepressed Ptet to crank out lots of light sensor protein
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type: SequenceAnnotation
persistent identity: annotation1747805
display id: annotation1747805
version: 1
top level: BBa_M30109
location: https://synbiohub.org/public/igem/BBa_M30109/annotation1747805/range1747805/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_M30109/annotation1747805/range1747805/1
type: Range
persistent identity: range1747805
display id: range1747805
version: 1
direction: direction/forward
top level: BBa_M30109
start: 4205
end: 4284
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1747742
display id: annotation1747742
version: 1
top level: BBa_M30109
location: https://synbiohub.org/public/igem/BBa_M30109/annotation1747742/range1747742/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_M30109/annotation1747742/range1747742/1
type: Range
persistent identity: range1747742
display id: range1747742
version: 1
direction: direction/forward
top level: BBa_M30109
start: 139
end: 150
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1747795
display id: annotation1747795
version: 1
top level: BBa_M30109
location: https://synbiohub.org/public/igem/BBa_M30109/annotation1747795/range1747795/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_M30109/annotation1747795/range1747795/1
type: Range
persistent identity: range1747795
display id: range1747795
version: 1
direction: direction/forward
top level: BBa_M30109
start: 1916
end: 1927
orientation: inline

BBa_I13453
type: SequenceAnnotation
persistent identity: annotation1747737
display id: annotation1747737
version: 1
top level: BBa_M30109
location: https://synbiohub.org/public/igem/BBa_M30109/annotation1747737/range1747737/1
component: BBa_I13453

https://synbiohub.org/public/igem/BBa_M30109/annotation1747737/range1747737/1
type: Range
persistent identity: range1747737
display id: range1747737
version: 1
direction: direction/forward
top level: BBa_M30109
start: 1
end: 130
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1747815
display id: annotation1747815
version: 1
top level: BBa_M30109
location: https://synbiohub.org/public/igem/BBa_M30109/annotation1747815/range1747815/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_M30109/annotation1747815/range1747815/1
type: Range
persistent identity: range1747815
display id: range1747815
version: 1
direction: direction/forward
top level: BBa_M30109
start: 4293
end: 4333
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1747752
display id: annotation1747752
version: 1
top level: BBa_M30109
location: https://synbiohub.org/public/igem/BBa_M30109/annotation1747752/range1747752/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_M30109/annotation1747752/range1747752/1
type: Range
persistent identity: range1747752
display id: range1747752
version: 1
direction: direction/forward
top level: BBa_M30109
start: 916
end: 927
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1747772
display id: annotation1747772
version: 1
top level: BBa_M30109
location: https://synbiohub.org/public/igem/BBa_M30109/annotation1747772/range1747772/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_M30109/annotation1747772/range1747772/1
type: Range
persistent identity: range1747772
display id: range1747772
version: 1
direction: direction/forward
top level: BBa_M30109
start: 1805
end: 1845
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1747787
display id: annotation1747787
version: 1
top level: BBa_M30109
location: https://synbiohub.org/public/igem/BBa_M30109/annotation1747787/range1747787/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_M30109/annotation1747787/range1747787/1
type: Range
persistent identity: range1747787
display id: range1747787
version: 1
direction: direction/forward
top level: BBa_M30109
start: 1854
end: 1907
orientation: inline

BBa_I15008
type: SequenceAnnotation
persistent identity: annotation1747746
display id: annotation1747746
version: 1
top level: BBa_M30109
location: https://synbiohub.org/public/igem/BBa_M30109/annotation1747746/range1747746/1
component: BBa_I15008

https://synbiohub.org/public/igem/BBa_M30109/annotation1747746/range1747746/1
type: Range
persistent identity: range1747746
display id: range1747746
version: 1
direction: direction/forward
top level: BBa_M30109
start: 157
end: 882
orientation: inline

BBa_I15010
type: SequenceAnnotation
persistent identity: annotation1747799
display id: annotation1747799
version: 1
top level: BBa_M30109
location: https://synbiohub.org/public/igem/BBa_M30109/annotation1747799/range1747799/1
component: BBa_I15010

https://synbiohub.org/public/igem/BBa_M30109/annotation1747799/range1747799/1
type: Range
persistent identity: range1747799
display id: range1747799
version: 1
direction: direction/forward
top level: BBa_M30109
start: 1934
end: 4171
orientation: inline

BBa_K864444
type: ComponentDefinition
persistent identity: BBa K864444
display id: BBa_K864444
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RFP-Linker-SYFP2
creator: Joel ??s
created: 2012-09-24T11:00:00Z
modified: 2015-05-08T01:13:37Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1124_
m user id: 0
owner id: 13998
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: Use this part to insert any 5?????TR of your gene of interest to screen for downregulation of your gene with small RNA. PCR amplify your 5???UTR with EcoRI and BamHI and clone into any backbone carrying this part. Digest whis part with EcoRI and BamHI and as RFP is replaced, this part functions as a red/white screening system. This allows you to create a reporter system with a fluorescent marker for screening for functional small RNAs. SYFP2 BBa_K864100 is situated downstrean of the RFP BBa_J04450 and is connected in frame with a 12 aa glycine-serine repeated linker BBa_J18922 , to prevent folding distruptions in the YFP or the 5???UTR. The first 6 nt of the glycine-serine linker is a BamHI restriction site (GGATCC), which translates into glycine-serine in E.Coli. When screening for working small RNAs, it's recommended to include the first 15 codons of the gene of interest.
mutable notes: -
mutable provenance: Constructed from SYFP2 BBa_K864100 , linker BBa_J18922 and RFP BBa_J04450
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: RFP
sequence annotation: B0012
sequence annotation: B0010
sequence annotation: BamHI
sequence annotation: Plac
sequence annotation: Linker
sequence annotation: B0034
sequence annotation: RFP J04450
sequence annotation: SYFP2
sequence: https://synbiohub.org/public/igem/BBa_K864444_sequence/1

RFP
type: SequenceAnnotation
persistent identity: annotation2198887
display id: annotation2198887
version: 1
top level: BBa_K864444
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K864444/annotation2198887/range2198887/1

https://synbiohub.org/public/igem/BBa_K864444/annotation2198887/range2198887/1
type: Range
persistent identity: range2198887
display id: range2198887
version: 1
direction: direction/forward
top level: BBa_K864444
start: 227
end: 681
orientation: inline

B0012
type: SequenceAnnotation
persistent identity: annotation2198889
display id: annotation2198889
version: 1
top level: BBa_K864444
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K864444/annotation2198889/range2198889/1

https://synbiohub.org/public/igem/BBa_K864444/annotation2198889/range2198889/1
type: Range
persistent identity: range2198889
display id: range2198889
version: 1
direction: direction/forward
top level: BBa_K864444
start: 1029
end: 1069
orientation: inline

B0010
type: SequenceAnnotation
persistent identity: annotation2198890
display id: annotation2198890
version: 1
top level: BBa_K864444
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K864444/annotation2198890/range2198890/1

https://synbiohub.org/public/igem/BBa_K864444/annotation2198890/range2198890/1
type: Range
persistent identity: range2198890
display id: range2198890
version: 1
direction: direction/forward
top level: BBa_K864444
start: 941
end: 1020
orientation: inline

BamHI
type: SequenceAnnotation
persistent identity: annotation2198894
display id: annotation2198894
version: 1
top level: BBa_K864444
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864444/annotation2198894/range2198894/1

https://synbiohub.org/public/igem/BBa_K864444/annotation2198894/range2198894/1
type: Range
persistent identity: range2198894
display id: range2198894
version: 1
direction: direction/forward
top level: BBa_K864444
start: 1070
end: 1075
orientation: inline

Plac
type: SequenceAnnotation
persistent identity: annotation2198885
display id: annotation2198885
version: 1
top level: BBa_K864444
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K864444/annotation2198885/range2198885/1

https://synbiohub.org/public/igem/BBa_K864444/annotation2198885/range2198885/1
type: Range
persistent identity: range2198885
display id: range2198885
version: 1
direction: direction/forward
top level: BBa_K864444
start: 1
end: 200
orientation: inline

Linker
type: SequenceAnnotation
persistent identity: annotation2198893
display id: annotation2198893
version: 1
top level: BBa_K864444
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864444/annotation2198893/range2198893/1

https://synbiohub.org/public/igem/BBa_K864444/annotation2198893/range2198893/1
type: Range
persistent identity: range2198893
display id: range2198893
version: 1
direction: direction/forward
top level: BBa_K864444
start: 1076
end: 1105
orientation: inline

B0034
type: SequenceAnnotation
persistent identity: annotation2198886
display id: annotation2198886
version: 1
top level: BBa_K864444
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K864444/annotation2198886/range2198886/1

https://synbiohub.org/public/igem/BBa_K864444/annotation2198886/range2198886/1
type: Range
persistent identity: range2198886
display id: range2198886
version: 1
direction: direction/forward
top level: BBa_K864444
start: 209
end: 220
orientation: inline

RFP J04450
type: SequenceAnnotation
persistent identity: annotation2198883
display id: annotation2198883
version: 1
top level: BBa_K864444
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864444/annotation2198883/range2198883/1

https://synbiohub.org/public/igem/BBa_K864444/annotation2198883/range2198883/1
type: Range
persistent identity: range2198883
display id: range2198883
version: 1
direction: direction/forward
top level: BBa_K864444
start: 1
end: 1068
orientation: inline

SYFP2
type: SequenceAnnotation
persistent identity: annotation2198896
display id: annotation2198896
version: 1
top level: BBa_K864444
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K864444/annotation2198896/range2198896/1

https://synbiohub.org/public/igem/BBa_K864444/annotation2198896/range2198896/1
type: Range
persistent identity: range2198896
display id: range2198896
version: 1
direction: direction/forward
top level: BBa_K864444
start: 1106
end: 1825
orientation: inline

BBa_I13710
type: ComponentDefinition
persistent identity: BBa I13710
display id: BBa_I13710
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CheY behind a tet promoter
creator: Victoria Chou, Kenneth Nesmith, Madeleine Sheldon-Dante
created: 2004-08-12T11:00:00Z
modified: 2015-08-31T04:07:36Z
discontinued: false
dominant: false
experience: experience/None
group u list: _6_
m user id: 0
owner id: 101
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Tet promoter, Elowitz RBS, CheY coding. CheY is used in chemotaxis.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_R0040
component: BBa_B0015
component: BBa_C0020
component: BBa_B0034
sequence annotation: BBa_C0020
sequence annotation: BBa_B0034
sequence annotation: BBa_B0015
sequence annotation: BBa_R0040
sequence: https://synbiohub.org/public/igem/BBa_I13710_sequence/1

BBa_R0040
type: Component
persistent identity: component2221602
display id: component2221602
version: 1
top level: BBa_I13710
access: public
definition: https://synbiohub.org/public/igem/BBa_R0040/1

BBa_B0015
type: Component
persistent identity: component2221617
display id: component2221617
version: 1
top level: BBa_I13710
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_C0020
type: Component
persistent identity: component2221610
display id: component2221610
version: 1
top level: BBa_I13710
access: public
definition: https://synbiohub.org/public/igem/BBa_C0020/1

BBa_B0034
type: Component
persistent identity: component2221608
display id: component2221608
version: 1
top level: BBa_I13710
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_C0020
type: SequenceAnnotation
persistent identity: annotation2221610
display id: annotation2221610
version: 1
top level: BBa_I13710
location: https://synbiohub.org/public/igem/BBa_I13710/annotation2221610/range2221610/1
component: BBa_C0020

https://synbiohub.org/public/igem/BBa_I13710/annotation2221610/range2221610/1
type: Range
persistent identity: range2221610
display id: range2221610
version: 1
direction: direction/reverse
top level: BBa_I13710
start: 81
end: 473
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2221608
display id: annotation2221608
version: 1
top level: BBa_I13710
location: https://synbiohub.org/public/igem/BBa_I13710/annotation2221608/range2221608/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I13710/annotation2221608/range2221608/1
type: Range
persistent identity: range2221608
display id: range2221608
version: 1
direction: direction/reverse
top level: BBa_I13710
start: 63
end: 74
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2221617
display id: annotation2221617
version: 1
top level: BBa_I13710
location: https://synbiohub.org/public/igem/BBa_I13710/annotation2221617/range2221617/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_I13710/annotation2221617/range2221617/1
type: Range
persistent identity: range2221617
display id: range2221617
version: 1
direction: direction/reverse
top level: BBa_I13710
start: 482
end: 610
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation2221602
display id: annotation2221602
version: 1
top level: BBa_I13710
location: https://synbiohub.org/public/igem/BBa_I13710/annotation2221602/range2221602/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_I13710/annotation2221602/range2221602/1
type: Range
persistent identity: range2221602
display id: range2221602
version: 1
direction: direction/forward
top level: BBa_I13710
start: 1
end: 54
orientation: inline

BBa_I13520
type: ComponentDefinition
persistent identity: BBa I13520
display id: BBa_I13520
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Pbad mRFP
creator: jkm
created: 2005-06-13T11:00:00Z
modified: 2015-08-31T04:07:34Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _11_
m user id: 0
owner id: 253
owning group id: 6
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Pbad mRFP
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0034
component: BBa_B0012
component: BBa_E1010
component: BBa_I0500
component: BBa_B0010
sequence annotation: BBa_E1010
sequence annotation: BBa_B0012
sequence annotation: BBa_B0010
sequence annotation: BBa_I0500
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_I13520_sequence/1

BBa_B0034
type: Component
persistent identity: component1515617
display id: component1515617
version: 1
top level: BBa_I13520
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0012
type: Component
persistent identity: component1515640
display id: component1515640
version: 1
top level: BBa_I13520
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_E1010
type: Component
persistent identity: component1515624
display id: component1515624
version: 1
top level: BBa_I13520
access: public
definition: https://synbiohub.org/public/igem/BBa_E1010/1

BBa_I0500
type: Component
persistent identity: component1515612
display id: component1515612
version: 1
top level: BBa_I13520
access: public
definition: https://synbiohub.org/public/igem/BBa_I0500/1

BBa_B0010
type: Component
persistent identity: component1515630
display id: component1515630
version: 1
top level: BBa_I13520
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_E1010
type: SequenceAnnotation
persistent identity: annotation1515624
display id: annotation1515624
version: 1
top level: BBa_I13520
location: https://synbiohub.org/public/igem/BBa_I13520/annotation1515624/range1515624/1
component: BBa_E1010

https://synbiohub.org/public/igem/BBa_I13520/annotation1515624/range1515624/1
type: Range
persistent identity: range1515624
display id: range1515624
version: 1
direction: direction/forward
top level: BBa_I13520
start: 1237
end: 1917
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1515640
display id: annotation1515640
version: 1
top level: BBa_I13520
location: https://synbiohub.org/public/igem/BBa_I13520/annotation1515640/range1515640/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I13520/annotation1515640/range1515640/1
type: Range
persistent identity: range1515640
display id: range1515640
version: 1
direction: direction/forward
top level: BBa_I13520
start: 2039
end: 2079
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1515630
display id: annotation1515630
version: 1
top level: BBa_I13520
location: https://synbiohub.org/public/igem/BBa_I13520/annotation1515630/range1515630/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I13520/annotation1515630/range1515630/1
type: Range
persistent identity: range1515630
display id: range1515630
version: 1
direction: direction/forward
top level: BBa_I13520
start: 1951
end: 2030
orientation: inline

BBa_I0500
type: SequenceAnnotation
persistent identity: annotation1515612
display id: annotation1515612
version: 1
top level: BBa_I13520
location: https://synbiohub.org/public/igem/BBa_I13520/annotation1515612/range1515612/1
component: BBa_I0500

https://synbiohub.org/public/igem/BBa_I13520/annotation1515612/range1515612/1
type: Range
persistent identity: range1515612
display id: range1515612
version: 1
direction: direction/forward
top level: BBa_I13520
start: 1
end: 1210
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1515617
display id: annotation1515617
version: 1
top level: BBa_I13520
location: https://synbiohub.org/public/igem/BBa_I13520/annotation1515617/range1515617/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I13520/annotation1515617/range1515617/1
type: Range
persistent identity: range1515617
display id: range1515617
version: 1
direction: direction/forward
top level: BBa_I13520
start: 1219
end: 1230
orientation: inline

BBa_J06910
type: ComponentDefinition
persistent identity: BBa J06910
display id: BBa_J06910
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LacI QPI regulated by lambda cI with EYFP
creator: Yves Wang
created: 2005-07-31T11:00:00Z
modified: 2015-08-31T04:08:19Z
discontinued: false
dominant: false
group u list: _20_
m user id: 0
owner id: 340
owning group id: 20
sample status: Not in stock
status: status/Unavailable
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0010
component: BBa_E0030
component: BBa_C0012
component: BBa_B0034
component: BBa_B0012
component: BBa_R0011
component: BBa_R0051
sequence annotation: BBa_R0011
sequence annotation: BBa_E0030
sequence annotation: BBa_B0010
sequence annotation: BBa_C0012
sequence annotation: BBa_B0034
sequence annotation: BBa_R0051
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_J06910_sequence/1

BBa_B0010
type: Component
persistent identity: component1599107
display id: component1599107
version: 1
top level: BBa_J06910
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_E0030
type: Component
persistent identity: component1599142
display id: component1599142
version: 1
top level: BBa_J06910
access: public
definition: https://synbiohub.org/public/igem/BBa_E0030/1

BBa_C0012
type: Component
persistent identity: component1599100
display id: component1599100
version: 1
top level: BBa_J06910
access: public
definition: https://synbiohub.org/public/igem/BBa_C0012/1

BBa_B0034
type: Component
persistent identity: component1599090
display id: component1599090
version: 1
top level: BBa_J06910
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0012
type: Component
persistent identity: component1599117
display id: component1599117
version: 1
top level: BBa_J06910
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_R0011
type: Component
persistent identity: component1599131
display id: component1599131
version: 1
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description: TetR under Pbad control
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modified: 2015-08-31T04:07:32Z
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creator: Barry and Debra and Jay
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experience: experience/None
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sample status: Discontinued
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bookmark: false
mutable description: -- No description --
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experience: experience/None
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description: Test of pBAD/araC promoter (I0500)
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created: 2004-07-27T11:00:00Z
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modified: 2015-08-31T04:07:44Z
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description: Ring Oscillator Synchronization Device (I13260.Q04121.I0461)
creator: robertb
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modified: 2015-08-31T04:07:37Z
discontinued: false
dominant: false
experience: experience/None
group u list: _6_
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status: status/Unavailable
bookmark: false
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top level: BBa_I13920
location: https://synbiohub.org/public/igem/BBa_I13920/annotation2234944/range2234944/1
component: BBa_Q04121

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type: Range
persistent identity: range2234944
display id: range2234944
version: 1
direction: direction/reverse
top level: BBa_I13920
start: 1167
end: 2537
orientation: inline

BBa_F1610
type: SequenceAnnotation
persistent identity: annotation2234957
display id: annotation2234957
version: 1
top level: BBa_I13920
location: https://synbiohub.org/public/igem/BBa_I13920/annotation2234957/range2234957/1
component: BBa_F1610

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type: Range
persistent identity: range2234957
display id: range2234957
version: 1
direction: direction/reverse
top level: BBa_I13920
start: 2546
end: 3343
orientation: inline

BBa_R8237
type: ComponentDefinition
persistent identity: BBa R8237
display id: BBa_R8237
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Dedicated promoter and RBS
creator: Barry Canton
created: 2005-12-06T12:00:00Z
modified: 2015-05-08T01:14:16Z
discontinued: false
dominant: false
group u list: _11_6_
m user id: 0
owner id: 135
owning group id: 6
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is a composite part of a T7 promoter and a dedicated RBS. It will be used to construct a reporter device for VM1.0
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0037
component: BBa_R0182
sequence annotation: BBa_B0037
sequence annotation: BBa_R0182
sequence: https://synbiohub.org/public/igem/BBa_R8237_sequence/1

BBa_B0037
type: Component
persistent identity: component1760586
display id: component1760586
version: 1
top level: BBa_R8237
access: public
definition: https://synbiohub.org/public/igem/BBa_B0037/1

BBa_R0182
type: Component
persistent identity: component1760584
display id: component1760584
version: 1
top level: BBa_R8237
access: public
definition: https://synbiohub.org/public/igem/BBa_R0182/1

BBa_B0037
type: SequenceAnnotation
persistent identity: annotation1760586
display id: annotation1760586
version: 1
top level: BBa_R8237
location: https://synbiohub.org/public/igem/BBa_R8237/annotation1760586/range1760586/1
component: BBa_B0037

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type: Range
persistent identity: range1760586
display id: range1760586
version: 1
direction: direction/forward
top level: BBa_R8237
start: 32
end: 41
orientation: inline

BBa_R0182
type: SequenceAnnotation
persistent identity: annotation1760584
display id: annotation1760584
version: 1
top level: BBa_R8237
location: https://synbiohub.org/public/igem/BBa_R8237/annotation1760584/range1760584/1
component: BBa_R0182

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type: Range
persistent identity: range1760584
display id: range1760584
version: 1
direction: direction/forward
top level: BBa_R8237
start: 1
end: 23
orientation: inline

BBa_I0403
type: ComponentDefinition
persistent identity: BBa I0403
display id: BBa_I0403
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: I0500.B0034.C0060.E0420
creator: jcbraff
created: 2004-06-08T11:00:00Z
modified: 2015-08-31T04:07:28Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_1_
m user id: 0
owner id: 61
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: I0500.B0034.C0060.E0420
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0012
component: BBa_I0500
component: BBa_C0060
component: BBa_B0034
component: BBa_B0010
component: BBa_E0020
sequence annotation: BBa_C0060
sequence annotation: BBa_I0500
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_B0034
sequence annotation: BBa_E0020
sequence: https://synbiohub.org/public/igem/BBa_I0403_sequence/1

BBa_B0012
type: Component
persistent identity: component958557
display id: component958557
version: 1
top level: BBa_I0403
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_I0500
type: Component
persistent identity: component958512
display id: component958512
version: 1
top level: BBa_I0403
access: public
definition: https://synbiohub.org/public/igem/BBa_I0500/1

BBa_C0060
type: Component
persistent identity: component958532
display id: component958532
version: 1
top level: BBa_I0403
access: public
definition: https://synbiohub.org/public/igem/BBa_C0060/1

BBa_B0034
type: Component
persistent identity: component958540
display id: component958540
version: 1
top level: BBa_I0403
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0010
type: Component
persistent identity: component958547
display id: component958547
version: 1
top level: BBa_I0403
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_E0020
type: Component
persistent identity: component958542
display id: component958542
version: 1
top level: BBa_I0403
access: public
definition: https://synbiohub.org/public/igem/BBa_E0020/1

BBa_B0034
type: Component
persistent identity: component958517
display id: component958517
version: 1
top level: BBa_I0403
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_C0060
type: SequenceAnnotation
persistent identity: annotation958532
display id: annotation958532
version: 1
top level: BBa_I0403
location: https://synbiohub.org/public/igem/BBa_I0403/annotation958532/range958532/1
component: BBa_C0060

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type: Range
persistent identity: range958532
display id: range958532
version: 1
direction: direction/forward
top level: BBa_I0403
start: 1237
end: 2025
orientation: inline

BBa_I0500
type: SequenceAnnotation
persistent identity: annotation958512
display id: annotation958512
version: 1
top level: BBa_I0403
location: https://synbiohub.org/public/igem/BBa_I0403/annotation958512/range958512/1
component: BBa_I0500

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type: Range
persistent identity: range958512
display id: range958512
version: 1
direction: direction/forward
top level: BBa_I0403
start: 1
end: 1210
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation958547
display id: annotation958547
version: 1
top level: BBa_I0403
location: https://synbiohub.org/public/igem/BBa_I0403/annotation958547/range958547/1
component: BBa_B0010

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type: Range
persistent identity: range958547
display id: range958547
version: 1
direction: direction/forward
top level: BBa_I0403
start: 2808
end: 2887
orientation: inline

BBa_B0012
type: SequenceAnnotation
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display id: annotation958557
version: 1
top level: BBa_I0403
location: https://synbiohub.org/public/igem/BBa_I0403/annotation958557/range958557/1
component: BBa_B0012

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type: Range
persistent identity: range958557
display id: range958557
version: 1
direction: direction/forward
top level: BBa_I0403
start: 2896
end: 2936
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation958517
display id: annotation958517
version: 1
top level: BBa_I0403
location: https://synbiohub.org/public/igem/BBa_I0403/annotation958517/range958517/1
component: BBa_B0034

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type: Range
persistent identity: range958517
display id: range958517
version: 1
direction: direction/forward
top level: BBa_I0403
start: 1219
end: 1230
orientation: inline

BBa_E0020
type: SequenceAnnotation
persistent identity: annotation958542
display id: annotation958542
version: 1
top level: BBa_I0403
location: https://synbiohub.org/public/igem/BBa_I0403/annotation958542/range958542/1
component: BBa_E0020

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type: Range
persistent identity: range958542
display id: range958542
version: 1
direction: direction/forward
top level: BBa_I0403
start: 2077
end: 2799
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation958540
display id: annotation958540
version: 1
top level: BBa_I0403
location: https://synbiohub.org/public/igem/BBa_I0403/annotation958540/range958540/1
component: BBa_B0034

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type: Range
persistent identity: range958540
display id: range958540
version: 1
direction: direction/forward
top level: BBa_I0403
start: 2059
end: 2070
orientation: inline

BBa_I14013
type: ComponentDefinition
persistent identity: BBa I14013
display id: BBa_I14013
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: R79, B30, E32
creator: Vikram Vijayan, Allen Hsu, Lawrence Fomundam
created: 2004-07-29T11:00:00Z
modified: 2015-08-31T04:07:37Z
discontinued: false
dominant: false
experience: experience/None
group u list: _4_
m user id: 0
owner id: 171
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: p(Las) (R79), Strong RBS (B30), EYFP + LVA (E32)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_R0079
component: BBa_E0032
component: BBa_B0030
sequence annotation: BBa_E0032
sequence annotation: BBa_B0030
sequence annotation: BBa_R0079
sequence: https://synbiohub.org/public/igem/BBa_I14013_sequence/1

BBa_R0079
type: Component
persistent identity: component1015778
display id: component1015778
version: 1
top level: BBa_I14013
access: public
definition: https://synbiohub.org/public/igem/BBa_R0079/1

BBa_E0032
type: Component
persistent identity: component1015799
display id: component1015799
version: 1
top level: BBa_I14013
access: public
definition: https://synbiohub.org/public/igem/BBa_E0032/1

BBa_B0030
type: Component
persistent identity: component1015786
display id: component1015786
version: 1
top level: BBa_I14013
access: public
definition: https://synbiohub.org/public/igem/BBa_B0030/1

BBa_E0032
type: SequenceAnnotation
persistent identity: annotation1015799
display id: annotation1015799
version: 1
top level: BBa_I14013
location: https://synbiohub.org/public/igem/BBa_I14013/annotation1015799/range1015799/1
component: BBa_E0032

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type: Range
persistent identity: range1015799
display id: range1015799
version: 1
direction: direction/forward
top level: BBa_I14013
start: 187
end: 948
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation1015786
display id: annotation1015786
version: 1
top level: BBa_I14013
location: https://synbiohub.org/public/igem/BBa_I14013/annotation1015786/range1015786/1
component: BBa_B0030

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type: Range
persistent identity: range1015786
display id: range1015786
version: 1
direction: direction/forward
top level: BBa_I14013
start: 166
end: 180
orientation: inline

BBa_R0079
type: SequenceAnnotation
persistent identity: annotation1015778
display id: annotation1015778
version: 1
top level: BBa_I14013
location: https://synbiohub.org/public/igem/BBa_I14013/annotation1015778/range1015778/1
component: BBa_R0079

https://synbiohub.org/public/igem/BBa_I14013/annotation1015778/range1015778/1
type: Range
persistent identity: range1015778
display id: range1015778
version: 1
direction: direction/forward
top level: BBa_I14013
start: 1
end: 157
orientation: inline

BBa_I12011
type: ComponentDefinition
persistent identity: BBa I12011
display id: BBa_I12011
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Barkai-Leibler design experiment part B (p22cII)
creator: Hans
created: 2004-07-18T11:00:00Z
modified: 2015-08-31T04:07:31Z
discontinued: false
dominant: false
experience: experience/None
group u list: _3_
m user id: 0
owner id: 103
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This forms the repressor of a Barkai-Leibler oscillator.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0015
component: BBa_B0025
component: BBa_I12007
component: BBa_C0053
component: BBa_B0034
sequence annotation: BBa_I12007
sequence annotation: BBa_C0053
sequence annotation: BBa_B0015
sequence annotation: BBa_B0025
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_I12011_sequence/1

BBa_B0015
type: Component
persistent identity: component2223533
display id: component2223533
version: 1
top level: BBa_I12011
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_B0025
type: Component
persistent identity: component2223513
display id: component2223513
version: 1
top level: BBa_I12011
access: public
definition: https://synbiohub.org/public/igem/BBa_B0025/1

BBa_I12007
type: Component
persistent identity: component2223519
display id: component2223519
version: 1
top level: BBa_I12011
access: public
definition: https://synbiohub.org/public/igem/BBa_I12007/1

BBa_C0053
type: Component
persistent identity: component2223523
display id: component2223523
version: 1
top level: BBa_I12011
access: public
definition: https://synbiohub.org/public/igem/BBa_C0053/1

BBa_B0034
type: Component
persistent identity: component2223521
display id: component2223521
version: 1
top level: BBa_I12011
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_I12007
type: SequenceAnnotation
persistent identity: annotation2223519
display id: annotation2223519
version: 1
top level: BBa_I12011
location: https://synbiohub.org/public/igem/BBa_I12011/annotation2223519/range2223519/1
component: BBa_I12007

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type: Range
persistent identity: range2223519
display id: range2223519
version: 1
direction: direction/reverse
top level: BBa_I12011
start: 138
end: 219
orientation: inline

BBa_C0053
type: SequenceAnnotation
persistent identity: annotation2223523
display id: annotation2223523
version: 1
top level: BBa_I12011
location: https://synbiohub.org/public/igem/BBa_I12011/annotation2223523/range2223523/1
component: BBa_C0053

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type: Range
persistent identity: range2223523
display id: range2223523
version: 1
direction: direction/forward
top level: BBa_I12011
start: 246
end: 932
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2223533
display id: annotation2223533
version: 1
top level: BBa_I12011
location: https://synbiohub.org/public/igem/BBa_I12011/annotation2223533/range2223533/1
component: BBa_B0015

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type: Range
persistent identity: range2223533
display id: range2223533
version: 1
direction: direction/reverse
top level: BBa_I12011
start: 966
end: 1094
orientation: inline

BBa_B0025
type: SequenceAnnotation
persistent identity: annotation2223513
display id: annotation2223513
version: 1
top level: BBa_I12011
location: https://synbiohub.org/public/igem/BBa_I12011/annotation2223513/range2223513/1
component: BBa_B0025

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type: Range
persistent identity: range2223513
display id: range2223513
version: 1
direction: direction/reverse
top level: BBa_I12011
start: 1
end: 129
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2223521
display id: annotation2223521
version: 1
top level: BBa_I12011
location: https://synbiohub.org/public/igem/BBa_I12011/annotation2223521/range2223521/1
component: BBa_B0034

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type: Range
persistent identity: range2223521
display id: range2223521
version: 1
direction: direction/reverse
top level: BBa_I12011
start: 228
end: 239
orientation: inline

BBa_I13283
type: ComponentDefinition
persistent identity: BBa I13283
display id: BBa_I13283
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pLac controlled receiver/reporter device (LuxR-based, HSL driven)
creator: Barry Canton
created: 2004-08-08T11:00:00Z
modified: 2015-08-31T04:07:33Z
discontinued: true
dominant: false
experience: experience/None
group u list: _11_6_
m user id: 0
owner id: 135
owning group id: 7
sample status: Discontinued
status: status/Deleted
bookmark: false
mutable description: Composite part based on the I13262 protein generator. LacI regulated promoter activated luxR-based HSL receiver. pLux-R is driving a YFP output device.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0010
component: BBa_C0062
component: BBa_B0012
component: BBa_B0034
component: BBa_R0011
component: BBa_R0062
component: BBa_E0030
sequence annotation: BBa_B0034
sequence annotation: BBa_B0012
sequence annotation: BBa_B0010
sequence annotation: BBa_E0030
sequence annotation: BBa_R0062
sequence annotation: BBa_C0062
sequence annotation: BBa_R0011
sequence: https://synbiohub.org/public/igem/BBa_I13283_sequence/1

BBa_B0010
type: Component
persistent identity: component1039482
display id: component1039482
version: 1
top level: BBa_I13283
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0010
type: Component
persistent identity: component1039522
display id: component1039522
version: 1
top level: BBa_I13283
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_C0062
type: Component
persistent identity: component1039474
display id: component1039474
version: 1
top level: BBa_I13283
access: public
definition: https://synbiohub.org/public/igem/BBa_C0062/1

BBa_B0012
type: Component
persistent identity: component1039492
display id: component1039492
version: 1
top level: BBa_I13283
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0034
type: Component
persistent identity: component1039459
display id: component1039459
version: 1
top level: BBa_I13283
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_R0011
type: Component
persistent identity: component1039450
display id: component1039450
version: 1
top level: BBa_I13283
access: public
definition: https://synbiohub.org/public/igem/BBa_R0011/1

BBa_B0012
type: Component
persistent identity: component1039532
display id: component1039532
version: 1
top level: BBa_I13283
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0034
type: Component
persistent identity: component1039515
display id: component1039515
version: 1
top level: BBa_I13283
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_R0062
type: Component
persistent identity: component1039507
display id: component1039507
version: 1
top level: BBa_I13283
access: public
definition: https://synbiohub.org/public/igem/BBa_R0062/1

BBa_E0030
type: Component
persistent identity: component1039517
display id: component1039517
version: 1
top level: BBa_I13283
access: public
definition: https://synbiohub.org/public/igem/BBa_E0030/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1039515
display id: annotation1039515
version: 1
top level: BBa_I13283
location: https://synbiohub.org/public/igem/BBa_I13283/annotation1039515/range1039515/1
component: BBa_B0034

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type: Range
persistent identity: range1039515
display id: range1039515
version: 1
direction: direction/forward
top level: BBa_I13283
start: 1070
end: 1081
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1039532
display id: annotation1039532
version: 1
top level: BBa_I13283
location: https://synbiohub.org/public/igem/BBa_I13283/annotation1039532/range1039532/1
component: BBa_B0012

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type: Range
persistent identity: range1039532
display id: range1039532
version: 1
direction: direction/forward
top level: BBa_I13283
start: 1907
end: 1947
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1039482
display id: annotation1039482
version: 1
top level: BBa_I13283
location: https://synbiohub.org/public/igem/BBa_I13283/annotation1039482/range1039482/1
component: BBa_B0010

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type: Range
persistent identity: range1039482
display id: range1039482
version: 1
direction: direction/forward
top level: BBa_I13283
start: 870
end: 949
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1039492
display id: annotation1039492
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description: R0010.E0021.B0020.E0031
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modified: 2015-08-31T04:07:45Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
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sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Terminator Characterization Construct
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owned by: myers
star: false
type: DnaRegion
role: SO:0000804
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component: BBa_E0021
component: BBa_B0020
component: BBa_R0010
component: BBa_E0031
sequence annotation: BBa_E0031
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sequence annotation: BBa_E0021
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definition: https://synbiohub.org/public/igem/BBa_B0020/1

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definition: https://synbiohub.org/public/igem/BBa_R0010/1

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definition: https://synbiohub.org/public/igem/BBa_E0031/1

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BBa_I12230
type: ComponentDefinition
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display id: BBa_I12230
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Two-hybrid system test construct
creator: rwald
created: 2004-08-06T11:00:00Z
modified: 2015-08-31T04:07:32Z
discontinued: false
dominant: false
experience: experience/None
group u list: _3_
m user id: 0
owner id: 120
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sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is a test construct for the two-hybrid system.
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owned by: myers
star: false
type: DnaRegion
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component: BBa_R0053
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component: BBa_I12224
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component: BBa_I12220
component: BBa_I12210
component: BBa_I12222
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sequence annotation: BBa_R0053
sequence annotation: BBa_R0011
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sequence: https://synbiohub.org/public/igem/BBa_I12230_sequence/1

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BBa_R0053
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definition: https://synbiohub.org/public/igem/BBa_E0422/1

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definition: https://synbiohub.org/public/igem/BBa_I12224/1

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display id: BBa_I14026
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description: C4, LacI Router (strong RBS)
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modified: 2015-08-31T04:07:37Z
discontinued: false
dominant: false
experience: experience/None
group u list: _4_
m user id: 0
owner id: 171
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: p(Rhl), Strong RBS, LacI + LVA
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
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component: BBa_C0012
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version: 1
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access: public
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version: 1
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access: public
definition: https://synbiohub.org/public/igem/BBa_I14017/1

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version: 1
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BBa_J06008
type: ComponentDefinition
persistent identity: BBa J06008
display id: BBa_J06008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: YFP and LuxI(LVA-) on LuxBox
creator: conniec
created: 2005-06-12T11:00:00Z
modified: 2015-08-31T04:08:16Z
discontinued: false
dominant: false
experience: experience/None
group u list: _20_
m user id: 0
owner id: 344
owning group id: 20
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Same as J06007, but with LVA- version of LuxI.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
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component: BBa_E0034
component: BBa_B0034
component: BBa_C0161
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component: BBa_B0010
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definition: https://synbiohub.org/public/igem/BBa_E0034/1

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BBa_C0161
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BBa_R0065
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description: Olmecs: Repressilator version 4 (P22 c2)
creator: Olmecs 2003 IAP Team
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top level: BBa_I5230
location: https://synbiohub.org/public/igem/BBa_I5230/annotation2234485/range2234485/1
component: BBa_B0011

https://synbiohub.org/public/igem/BBa_I5230/annotation2234485/range2234485/1
type: Range
persistent identity: range2234485
display id: range2234485
version: 1
direction: direction/forward
top level: BBa_I5230
start: 3071
end: 3116
orientation: inline

BBa_B0011
type: SequenceAnnotation
persistent identity: annotation2234466
display id: annotation2234466
version: 1
top level: BBa_I5230
location: https://synbiohub.org/public/igem/BBa_I5230/annotation2234466/range2234466/1
component: BBa_B0011

https://synbiohub.org/public/igem/BBa_I5230/annotation2234466/range2234466/1
type: Range
persistent identity: range2234466
display id: range2234466
version: 1
direction: direction/forward
top level: BBa_I5230
start: 1727
end: 1772
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2234456
display id: annotation2234456
version: 1
top level: BBa_I5230
location: https://synbiohub.org/public/igem/BBa_I5230/annotation2234456/range2234456/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I5230/annotation2234456/range2234456/1
type: Range
persistent identity: range2234456
display id: range2234456
version: 1
direction: direction/reverse
top level: BBa_I5230
start: 940
end: 951
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2234444
display id: annotation2234444
version: 1
top level: BBa_I5230
location: https://synbiohub.org/public/igem/BBa_I5230/annotation2234444/range2234444/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I5230/annotation2234444/range2234444/1
type: Range
persistent identity: range2234444
display id: range2234444
version: 1
direction: direction/forward
top level: BBa_I5230
start: 775
end: 815
orientation: inline

BBa_I14055
type: ComponentDefinition
persistent identity: BBa I14055
display id: BBa_I14055
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Tet Toggle (medium RBS, weak RBS)
creator: Vikram Vijayan, Allen Hsu, Lawrence Fomundam
created: 2004-08-03T11:00:00Z
modified: 2015-08-31T04:07:38Z
discontinued: false
dominant: false
experience: experience/None
group u list: _4_
m user id: 0
owner id: 171
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: p(Ltet0-1), Medium RBS, LacI + LVA, Weak RBS, Lambda CI + LVA, Strong RBS, EYFP + LVA
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_R0040
component: BBa_B0031
component: BBa_C0051
component: BBa_E0032
component: BBa_B0032
component: BBa_C0012
component: BBa_B0030
sequence annotation: BBa_B0032
sequence annotation: BBa_C0012
sequence annotation: BBa_B0030
sequence annotation: BBa_B0031
sequence annotation: BBa_E0032
sequence annotation: BBa_C0051
sequence annotation: BBa_R0040
sequence: https://synbiohub.org/public/igem/BBa_I14055_sequence/1

BBa_R0040
type: Component
persistent identity: component1029343
display id: component1029343
version: 1
top level: BBa_I14055
access: public
definition: https://synbiohub.org/public/igem/BBa_R0040/1

BBa_B0031
type: Component
persistent identity: component1029372
display id: component1029372
version: 1
top level: BBa_I14055
access: public
definition: https://synbiohub.org/public/igem/BBa_B0031/1

BBa_C0051
type: Component
persistent identity: component1029382
display id: component1029382
version: 1
top level: BBa_I14055
access: public
definition: https://synbiohub.org/public/igem/BBa_C0051/1

BBa_E0032
type: Component
persistent identity: component1029404
display id: component1029404
version: 1
top level: BBa_I14055
access: public
definition: https://synbiohub.org/public/igem/BBa_E0032/1

BBa_B0032
type: Component
persistent identity: component1029354
display id: component1029354
version: 1
top level: BBa_I14055
access: public
definition: https://synbiohub.org/public/igem/BBa_B0032/1

BBa_C0012
type: Component
persistent identity: component1029365
display id: component1029365
version: 1
top level: BBa_I14055
access: public
definition: https://synbiohub.org/public/igem/BBa_C0012/1

BBa_B0030
type: Component
persistent identity: component1029391
display id: component1029391
version: 1
top level: BBa_I14055
access: public
definition: https://synbiohub.org/public/igem/BBa_B0030/1

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation1029354
display id: annotation1029354
version: 1
top level: BBa_I14055
location: https://synbiohub.org/public/igem/BBa_I14055/annotation1029354/range1029354/1
component: BBa_B0032

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type: Range
persistent identity: range1029354
display id: range1029354
version: 1
direction: direction/forward
top level: BBa_I14055
start: 63
end: 75
orientation: inline

BBa_C0012
type: SequenceAnnotation
persistent identity: annotation1029365
display id: annotation1029365
version: 1
top level: BBa_I14055
location: https://synbiohub.org/public/igem/BBa_I14055/annotation1029365/range1029365/1
component: BBa_C0012

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type: Range
persistent identity: range1029365
display id: range1029365
version: 1
direction: direction/forward
top level: BBa_I14055
start: 82
end: 1209
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation1029391
display id: annotation1029391
version: 1
top level: BBa_I14055
location: https://synbiohub.org/public/igem/BBa_I14055/annotation1029391/range1029391/1
component: BBa_B0030

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type: Range
persistent identity: range1029391
display id: range1029391
version: 1
direction: direction/forward
top level: BBa_I14055
start: 2046
end: 2060
orientation: inline

BBa_B0031
type: SequenceAnnotation
persistent identity: annotation1029372
display id: annotation1029372
version: 1
top level: BBa_I14055
location: https://synbiohub.org/public/igem/BBa_I14055/annotation1029372/range1029372/1
component: BBa_B0031

https://synbiohub.org/public/igem/BBa_I14055/annotation1029372/range1029372/1
type: Range
persistent identity: range1029372
display id: range1029372
version: 1
direction: direction/forward
top level: BBa_I14055
start: 1243
end: 1256
orientation: inline

BBa_E0032
type: SequenceAnnotation
persistent identity: annotation1029404
display id: annotation1029404
version: 1
top level: BBa_I14055
location: https://synbiohub.org/public/igem/BBa_I14055/annotation1029404/range1029404/1
component: BBa_E0032

https://synbiohub.org/public/igem/BBa_I14055/annotation1029404/range1029404/1
type: Range
persistent identity: range1029404
display id: range1029404
version: 1
direction: direction/forward
top level: BBa_I14055
start: 2067
end: 2828
orientation: inline

BBa_C0051
type: SequenceAnnotation
persistent identity: annotation1029382
display id: annotation1029382
version: 1
top level: BBa_I14055
location: https://synbiohub.org/public/igem/BBa_I14055/annotation1029382/range1029382/1
component: BBa_C0051

https://synbiohub.org/public/igem/BBa_I14055/annotation1029382/range1029382/1
type: Range
persistent identity: range1029382
display id: range1029382
version: 1
direction: direction/forward
top level: BBa_I14055
start: 1263
end: 2012
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1029343
display id: annotation1029343
version: 1
top level: BBa_I14055
location: https://synbiohub.org/public/igem/BBa_I14055/annotation1029343/range1029343/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_I14055/annotation1029343/range1029343/1
type: Range
persistent identity: range1029343
display id: range1029343
version: 1
direction: direction/forward
top level: BBa_I14055
start: 1
end: 54
orientation: inline

pen LacI
type: ComponentDefinition
persistent identity: BBa I14008
display id: BBa_I14008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: p(pen) . strong RBS . LacI
creator: Aditi Shrivastava, Jiwon Lee, Sarah Welch
created: 2004-07-27T11:00:00Z
modified: 2015-08-31T04:07:37Z
discontinued: true
dominant: false
experience: experience/None
group u list: _4_
m user id: 0
owner id: 166
owning group id: 7
sample status: Discontinued
status: status/Deleted
bookmark: false
mutable description: BBa_R0074 BBa_I14002
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_I14008_sequence/1

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation1018068
display id: annotation1018068
version: 1
top level: pen LacI
role: SO:0000807
role: feature/barcode
location: https://synbiohub.org/public/igem/BBa_I14008/annotation1018068/range1018068/1

https://synbiohub.org/public/igem/BBa_I14008/annotation1018068/range1018068/1
type: Range
persistent identity: range1018068
display id: range1018068
version: 1
direction: direction/forward
top level: pen LacI
start: 1
end: 25
orientation: inline

BBa_I14054
type: ComponentDefinition
persistent identity: BBa I14054
display id: BBa_I14054
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Lac Toggle (medium RBS)
creator: Vikram Vijayan, Allen Hsu, Lawrence Fomundam
created: 2004-08-03T11:00:00Z
modified: 2015-08-31T04:07:38Z
discontinued: false
dominant: false
experience: experience/None
group u list: _4_
m user id: 0
owner id: 171
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: p(Lac), Medium RBS, TetR + LVA, Strong RBS, ECFP + LVA
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0030
component: BBa_C0040
component: BBa_R0010
component: BBa_E0022
component: BBa_B0032
sequence annotation: BBa_C0040
sequence annotation: BBa_B0032
sequence annotation: BBa_E0022
sequence annotation: BBa_B0030
sequence annotation: BBa_R0010
sequence: https://synbiohub.org/public/igem/BBa_I14054_sequence/1

BBa_B0030
type: Component
persistent identity: component1029311
display id: component1029311
version: 1
top level: BBa_I14054
access: public
definition: https://synbiohub.org/public/igem/BBa_B0030/1

BBa_C0040
type: Component
persistent identity: component1029302
display id: component1029302
version: 1
top level: BBa_I14054
access: public
definition: https://synbiohub.org/public/igem/BBa_C0040/1

BBa_R0010
type: Component
persistent identity: component1029278
display id: component1029278
version: 1
top level: BBa_I14054
access: public
definition: https://synbiohub.org/public/igem/BBa_R0010/1

BBa_E0022
type: Component
persistent identity: component1029324
display id: component1029324
version: 1
top level: BBa_I14054
access: public
definition: https://synbiohub.org/public/igem/BBa_E0022/1

BBa_B0032
type: Component
persistent identity: component1029291
display id: component1029291
version: 1
top level: BBa_I14054
access: public
definition: https://synbiohub.org/public/igem/BBa_B0032/1

BBa_C0040
type: SequenceAnnotation
persistent identity: annotation1029302
display id: annotation1029302
version: 1
top level: BBa_I14054
location: https://synbiohub.org/public/igem/BBa_I14054/annotation1029302/range1029302/1
component: BBa_C0040

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type: Range
persistent identity: range1029302
display id: range1029302
version: 1
direction: direction/forward
top level: BBa_I14054
start: 228
end: 887
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation1029291
display id: annotation1029291
version: 1
top level: BBa_I14054
location: https://synbiohub.org/public/igem/BBa_I14054/annotation1029291/range1029291/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_I14054/annotation1029291/range1029291/1
type: Range
persistent identity: range1029291
display id: range1029291
version: 1
direction: direction/forward
top level: BBa_I14054
start: 209
end: 221
orientation: inline

BBa_E0022
type: SequenceAnnotation
persistent identity: annotation1029324
display id: annotation1029324
version: 1
top level: BBa_I14054
location: https://synbiohub.org/public/igem/BBa_I14054/annotation1029324/range1029324/1
component: BBa_E0022

https://synbiohub.org/public/igem/BBa_I14054/annotation1029324/range1029324/1
type: Range
persistent identity: range1029324
display id: range1029324
version: 1
direction: direction/forward
top level: BBa_I14054
start: 942
end: 1703
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation1029311
display id: annotation1029311
version: 1
top level: BBa_I14054
location: https://synbiohub.org/public/igem/BBa_I14054/annotation1029311/range1029311/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_I14054/annotation1029311/range1029311/1
type: Range
persistent identity: range1029311
display id: range1029311
version: 1
direction: direction/forward
top level: BBa_I14054
start: 921
end: 935
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation1029278
display id: annotation1029278
version: 1
top level: BBa_I14054
location: https://synbiohub.org/public/igem/BBa_I14054/annotation1029278/range1029278/1
component: BBa_R0010

https://synbiohub.org/public/igem/BBa_I14054/annotation1029278/range1029278/1
type: Range
persistent identity: range1029278
display id: range1029278
version: 1
direction: direction/forward
top level: BBa_I14054
start: 1
end: 200
orientation: inline

BBa_I13510
type: ComponentDefinition
persistent identity: BBa I13510
display id: BBa_I13510
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Screening plasmid intermediate
creator: jkm
created: 2005-05-30T11:00:00Z
modified: 2015-08-31T04:07:34Z
discontinued: true
dominant: false
experience: experience/None
group u list: _11_
m user id: 0
owner id: 253
owning group id: 6
part status: Released HQ 2013
sample status: Discontinued
status: status/Deleted
bookmark: false
mutable description: Built by Josh as an intermediate in screening plasmid construction.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0012
component: BBa_I13452
component: BBa_B0010
component: BBa_B0034
component: BBa_E0040
sequence annotation: BBa_E0040
sequence annotation: BBa_I13452
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_I13510_sequence/1

BBa_B0012
type: Component
persistent identity: component1499340
display id: component1499340
version: 1
top level: BBa_I13510
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_I13452
type: Component
persistent identity: component1499318
display id: component1499318
version: 1
top level: BBa_I13510
access: public
definition: https://synbiohub.org/public/igem/BBa_I13452/1

BBa_B0010
type: Component
persistent identity: component1499330
display id: component1499330
version: 1
top level: BBa_I13510
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0034
type: Component
persistent identity: component1499323
display id: component1499323
version: 1
top level: BBa_I13510
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_E0040
type: Component
persistent identity: component1499325
display id: component1499325
version: 1
top level: BBa_I13510
access: public
definition: https://synbiohub.org/public/igem/BBa_E0040/1

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation1499325
display id: annotation1499325
version: 1
top level: BBa_I13510
location: https://synbiohub.org/public/igem/BBa_I13510/annotation1499325/range1499325/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_I13510/annotation1499325/range1499325/1
type: Range
persistent identity: range1499325
display id: range1499325
version: 1
direction: direction/forward
top level: BBa_I13510
start: 79
end: 798
orientation: inline

BBa_I13452
type: SequenceAnnotation
persistent identity: annotation1499318
display id: annotation1499318
version: 1
top level: BBa_I13510
location: https://synbiohub.org/public/igem/BBa_I13510/annotation1499318/range1499318/1
component: BBa_I13452

https://synbiohub.org/public/igem/BBa_I13510/annotation1499318/range1499318/1
type: Range
persistent identity: range1499318
display id: range1499318
version: 1
direction: direction/forward
top level: BBa_I13510
start: 1
end: 52
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1499330
display id: annotation1499330
version: 1
top level: BBa_I13510
location: https://synbiohub.org/public/igem/BBa_I13510/annotation1499330/range1499330/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I13510/annotation1499330/range1499330/1
type: Range
persistent identity: range1499330
display id: range1499330
version: 1
direction: direction/forward
top level: BBa_I13510
start: 807
end: 886
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1499340
display id: annotation1499340
version: 1
top level: BBa_I13510
location: https://synbiohub.org/public/igem/BBa_I13510/annotation1499340/range1499340/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I13510/annotation1499340/range1499340/1
type: Range
persistent identity: range1499340
display id: range1499340
version: 1
direction: direction/forward
top level: BBa_I13510
start: 895
end: 935
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1499323
display id: annotation1499323
version: 1
top level: BBa_I13510
location: https://synbiohub.org/public/igem/BBa_I13510/annotation1499323/range1499323/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I13510/annotation1499323/range1499323/1
type: Range
persistent identity: range1499323
display id: range1499323
version: 1
direction: direction/forward
top level: BBa_I13510
start: 61
end: 72
orientation: inline

BBa_I11062
type: ComponentDefinition
persistent identity: BBa I11062
display id: BBa_I11062
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: HK022 half bit
creator: bu
created: 2004-09-07T11:00:00Z
modified: 2015-08-31T04:07:30Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2_
m user id: 0
owner id: 102
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This half bit contains the integrase/excisionase for HK022 and attP/attB rev comp for P21. The attachment sites flank a unidirectional terminator. This half bit is designed to work with the P21 half bit (BBa_I11063)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: spacer
sequence annotation: PvuI
sequence annotation: LVA
sequence annotation: KpnI
sequence annotation: stop
sequence annotation: NotI
sequence annotation: cI HK022
sequence annotation: P21 attP attachment site (BBa_I11053)
sequence annotation: B0025 reverse terminator
sequence annotation: BmtI
sequence annotation: RBS
sequence annotation: AAV
sequence annotation: XmaI
sequence annotation: P21 attB (I11053)
sequence annotation: Integrase HK022+LVA (BBa_I11040)
sequence annotation: AclI
sequence: https://synbiohub.org/public/igem/BBa_I11062_sequence/1

spacer
type: SequenceAnnotation
persistent identity: annotation1197193
display id: annotation1197193
version: 1
top level: BBa_I11062
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I11062/annotation1197193/range1197193/1

https://synbiohub.org/public/igem/BBa_I11062/annotation1197193/range1197193/1
type: Range
persistent identity: range1197193
display id: range1197193
version: 1
direction: direction/forward
top level: BBa_I11062
start: 1737
end: 1739
orientation: inline

spacer
type: SequenceAnnotation
persistent identity: annotation1196588
display id: annotation1196588
version: 1
top level: BBa_I11062
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I11062/annotation1196588/range1196588/1

https://synbiohub.org/public/igem/BBa_I11062/annotation1196588/range1196588/1
type: Range
persistent identity: range1196588
display id: range1196588
version: 1
direction: direction/forward
top level: BBa_I11062
start: 19
end: 23
orientation: inline

PvuI
type: SequenceAnnotation
persistent identity: annotation1196917
display id: annotation1196917
version: 1
top level: BBa_I11062
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I11062/annotation1196917/range1196917/1

https://synbiohub.org/public/igem/BBa_I11062/annotation1196917/range1196917/1
type: Range
persistent identity: range1196917
display id: range1196917
version: 1
direction: direction/forward
top level: BBa_I11062
start: 1567
end: 1572
orientation: inline

spacer
type: SequenceAnnotation
persistent identity: annotation1196718
display id: annotation1196718
version: 1
top level: BBa_I11062
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I11062/annotation1196718/range1196718/1

https://synbiohub.org/public/igem/BBa_I11062/annotation1196718/range1196718/1
type: Range
persistent identity: range1196718
display id: range1196718
version: 1
direction: direction/forward
top level: BBa_I11062
start: 1134
end: 1136
orientation: inline

spacer
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LVA
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KpnI
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stop
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NotI
type: SequenceAnnotation
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role: SO:0000110
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cI HK022
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P21 attP attachment site (BBa_I11053)
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B0025 reverse terminator
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stop
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spacer
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BmtI
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spacer
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spacer
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spacer
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RBS
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spacer
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RBS
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AAV
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spacer
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XmaI
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P21 attB (I11053)
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spacer
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Integrase HK022+LVA (BBa_I11040)
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spacer
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AclI
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spacer
type: SequenceAnnotation
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BBa_I12233
type: ComponentDefinition
persistent identity: BBa I12233
display id: BBa_I12233
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TetR heterodimer test construct 2
creator: rwald
created: 2004-08-31T11:00:00Z
modified: 2015-08-31T04:07:32Z
discontinued: false
dominant: false
experience: experience/None
group u list: _3_
m user id: 0
owner id: 120
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Same as the other TetR heterodimer test construct (I12232), but with different promoters in front of the two components of the heterodimer.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_I12212
component: BBa_B0012
component: BBa_R0053
component: BBa_B0010
component: BBa_R0010
component: BBa_C0013
component: BBa_E0022
component: BBa_I12203
component: BBa_B0032
component: BBa_I12205
component: BBa_I0500
component: BBa_B0034
component: BBa_I12202
sequence annotation: BBa_R0053
sequence annotation: BBa_B0010
sequence annotation: BBa_B0032
sequence annotation: BBa_B0012
sequence annotation: BBa_B0034
sequence annotation: BBa_I12202
sequence annotation: BBa_I12212
sequence annotation: BBa_I0500
sequence annotation: BBa_I12205
sequence annotation: BBa_E0022
sequence annotation: BBa_C0013
sequence annotation: BBa_I12203
sequence annotation: BBa_R0010
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BBa_I12212
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BBa_B0012
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BBa_R0053
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BBa_B0010
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BBa_R0010
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BBa_B0010
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BBa_C0013
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BBa_E0022
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BBa_B0012
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BBa_I12203
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BBa_B0032
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BBa_I12205
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BBa_I0500
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BBa_B0012
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BBa_B0034
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BBa_B0010
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BBa_B0034
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BBa_R0053
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BBa_B0012
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BBa_B0032
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BBa_B0012
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BBa_B0010
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BBa_B0032
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BBa_R0053
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BBa_B0010
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BBa_B0032
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was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: R1 system A
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created: 2005-09-14T11:00:00Z
modified: 2015-08-31T04:08:15Z
discontinued: true
dominant: false
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sample status: Discontinued
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description: PoPS->PoPS amplifier (RhlR-based, HSL driven)
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created: 2004-08-08T11:00:00Z
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experience: experience/None
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sample status: Not in stock
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bookmark: false
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type: ComponentDefinition
persistent identity: BBa I14023
display id: BBa_I14023
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Constitutive LasR
creator: Vikram Vijayan, Allen Hsu, Lawrence Fomundam
created: 2004-08-01T11:00:00Z
modified: 2015-08-31T04:07:37Z
discontinued: false
dominant: false
experience: experience/None
group u list: _4_
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status: status/Unavailable
bookmark: false
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star: false
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access: public
definition: https://synbiohub.org/public/igem/BBa_B0030/1

BBa_C0079
type: Component
persistent identity: component1028584
display id: component1028584
version: 1
top level: BBa_I14023
access: public
definition: https://synbiohub.org/public/igem/BBa_C0079/1

BBa_C0079
type: SequenceAnnotation
persistent identity: annotation1028584
display id: annotation1028584
version: 1
top level: BBa_I14023
location: https://synbiohub.org/public/igem/BBa_I14023/annotation1028584/range1028584/1
component: BBa_C0079

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type: Range
persistent identity: range1028584
display id: range1028584
version: 1
direction: direction/forward
top level: BBa_I14023
start: 65
end: 820
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation1028570
display id: annotation1028570
version: 1
top level: BBa_I14023
location: https://synbiohub.org/public/igem/BBa_I14023/annotation1028570/range1028570/1
component: BBa_B0030

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type: Range
persistent identity: range1028570
display id: range1028570
version: 1
direction: direction/forward
top level: BBa_I14023
start: 44
end: 58
orientation: inline

BBa_I14018
type: SequenceAnnotation
persistent identity: annotation1028562
display id: annotation1028562
version: 1
top level: BBa_I14023
location: https://synbiohub.org/public/igem/BBa_I14023/annotation1028562/range1028562/1
component: BBa_I14018

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type: Range
persistent identity: range1028562
display id: range1028562
version: 1
direction: direction/forward
top level: BBa_I14023
start: 1
end: 35
orientation: inline

BBa_I12051
type: ComponentDefinition
persistent identity: BBa I12051
display id: BBa_I12051
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: (New and improved) Test circuit for I12006
creator: endelman
created: 2004-09-02T11:00:00Z
modified: 2015-08-31T04:07:31Z
discontinued: false
dominant: false
experience: experience/None
group u list: _3_
m user id: 0
owner id: 145
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: IPTG and arabinose are used to control expression of lambda cI/LVA and 434 cI, respectively. These two repressors bind into I12006 and modulate expression of YFP/AAV. Must be used with LacIq strain.
mutable notes: This part links together generators for lambda cI and 434 cI with I12006 and YFP reporter.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_I12050
component: BBa_I12006
component: BBa_E0434
component: BBa_I12024
sequence annotation: BBa_I12050
sequence annotation: BBa_I12006
sequence annotation: BBa_I12024
sequence annotation: BBa_E0434
sequence: https://synbiohub.org/public/igem/BBa_I12051_sequence/1

BBa_I12050
type: Component
persistent identity: component2235040
display id: component2235040
version: 1
top level: BBa_I12051
access: public
definition: https://synbiohub.org/public/igem/BBa_I12050/1

BBa_I12006
type: Component
persistent identity: component2235046
display id: component2235046
version: 1
top level: BBa_I12051
access: public
definition: https://synbiohub.org/public/igem/BBa_I12006/1

BBa_E0434
type: Component
persistent identity: component2235056
display id: component2235056
version: 1
top level: BBa_I12051
access: public
definition: https://synbiohub.org/public/igem/BBa_E0434/1

BBa_I12024
type: Component
persistent identity: component2235027
display id: component2235027
version: 1
top level: BBa_I12051
access: public
definition: https://synbiohub.org/public/igem/BBa_I12024/1

BBa_I12050
type: SequenceAnnotation
persistent identity: annotation2235040
display id: annotation2235040
version: 1
top level: BBa_I12051
location: https://synbiohub.org/public/igem/BBa_I12051/annotation2235040/range2235040/1
component: BBa_I12050

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type: Range
persistent identity: range2235040
display id: range2235040
version: 1
direction: direction/reverse
top level: BBa_I12051
start: 1002
end: 3010
orientation: inline

BBa_I12006
type: SequenceAnnotation
persistent identity: annotation2235046
display id: annotation2235046
version: 1
top level: BBa_I12051
location: https://synbiohub.org/public/igem/BBa_I12051/annotation2235046/range2235046/1
component: BBa_I12006

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type: Range
persistent identity: range2235046
display id: range2235046
version: 1
direction: direction/reverse
top level: BBa_I12051
start: 3019
end: 3100
orientation: inline

BBa_I12024
type: SequenceAnnotation
persistent identity: annotation2235027
display id: annotation2235027
version: 1
top level: BBa_I12051
location: https://synbiohub.org/public/igem/BBa_I12051/annotation2235027/range2235027/1
component: BBa_I12024

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persistent identity: range2235027
display id: range2235027
version: 1
direction: direction/reverse
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start: 1
end: 993
orientation: inline

BBa_E0434
type: SequenceAnnotation
persistent identity: annotation2235056
display id: annotation2235056
version: 1
top level: BBa_I12051
location: https://synbiohub.org/public/igem/BBa_I12051/annotation2235056/range2235056/1
component: BBa_E0434

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type: Range
persistent identity: range2235056
display id: range2235056
version: 1
direction: direction/reverse
top level: BBa_I12051
start: 3109
end: 4025
orientation: inline

BBa_I14060
type: ComponentDefinition
persistent identity: BBa I14060
display id: BBa_I14060
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Constitutive RhlR using P(Lac) IQ
creator: Vikram Vijayan, Allen Hsu, Lawrence Fomundam
created: 2004-08-04T11:00:00Z
modified: 2015-08-31T04:07:38Z
discontinued: false
dominant: false
experience: experience/None
group u list: _4_
m user id: 0
owner id: 171
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: p(Lac) IQ, Medium RBS, RhlR + LVA
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_C0071
component: BBa_B0032
component: BBa_I14032
sequence annotation: BBa_I14032
sequence annotation: BBa_B0032
sequence annotation: BBa_C0071
sequence: https://synbiohub.org/public/igem/BBa_I14060_sequence/1

BBa_C0071
type: Component
persistent identity: component1029646
display id: component1029646
version: 1
top level: BBa_I14060
access: public
definition: https://synbiohub.org/public/igem/BBa_C0071/1

BBa_B0032
type: Component
persistent identity: component1029635
display id: component1029635
version: 1
top level: BBa_I14060
access: public
definition: https://synbiohub.org/public/igem/BBa_B0032/1

BBa_I14032
type: Component
persistent identity: component1029627
display id: component1029627
version: 1
top level: BBa_I14060
access: public
definition: https://synbiohub.org/public/igem/BBa_I14032/1

BBa_I14032
type: SequenceAnnotation
persistent identity: annotation1029627
display id: annotation1029627
version: 1
top level: BBa_I14060
location: https://synbiohub.org/public/igem/BBa_I14060/annotation1029627/range1029627/1
component: BBa_I14032

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type: Range
persistent identity: range1029627
display id: range1029627
version: 1
direction: direction/forward
top level: BBa_I14060
start: 1
end: 37
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation1029635
display id: annotation1029635
version: 1
top level: BBa_I14060
location: https://synbiohub.org/public/igem/BBa_I14060/annotation1029635/range1029635/1
component: BBa_B0032

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type: Range
persistent identity: range1029635
display id: range1029635
version: 1
direction: direction/forward
top level: BBa_I14060
start: 46
end: 58
orientation: inline

BBa_C0071
type: SequenceAnnotation
persistent identity: annotation1029646
display id: annotation1029646
version: 1
top level: BBa_I14060
location: https://synbiohub.org/public/igem/BBa_I14060/annotation1029646/range1029646/1
component: BBa_C0071

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type: Range
persistent identity: range1029646
display id: range1029646
version: 1
direction: direction/forward
top level: BBa_I14060
start: 65
end: 826
orientation: inline

BBa_J04480
type: ComponentDefinition
persistent identity: BBa J04480
display id: BBa_J04480
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: EYFP coding device with promoter, RBS, and terminator. No degradation tag.
creator: Kristen DeCelle
created: 2005-06-23T11:00:00Z
modified: 2015-08-31T04:08:14Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _16_
m user id: 0
owner id: 326
owning group id: 16
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: EYFP with promoter, RBS, and terminator. No degradation tag.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0010
component: BBa_E0030
component: BBa_R0010
component: BBa_B0034
component: BBa_B0012
sequence annotation: BBa_B0012
sequence annotation: BBa_B0010
sequence annotation: BBa_E0030
sequence annotation: BBa_R0010
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_J04480_sequence/1

BBa_B0010
type: Component
persistent identity: component1537389
display id: component1537389
version: 1
top level: BBa_J04480
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_E0030
type: Component
persistent identity: component1537384
display id: component1537384
version: 1
top level: BBa_J04480
access: public
definition: https://synbiohub.org/public/igem/BBa_E0030/1

BBa_R0010
type: Component
persistent identity: component1537372
display id: component1537372
version: 1
top level: BBa_J04480
access: public
definition: https://synbiohub.org/public/igem/BBa_R0010/1

BBa_B0034
type: Component
persistent identity: component1537382
display id: component1537382
version: 1
top level: BBa_J04480
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0012
type: Component
persistent identity: component1537399
display id: component1537399
version: 1
top level: BBa_J04480
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1537399
display id: annotation1537399
version: 1
top level: BBa_J04480
location: https://synbiohub.org/public/igem/BBa_J04480/annotation1537399/range1537399/1
component: BBa_B0012

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persistent identity: range1537399
display id: range1537399
version: 1
direction: direction/forward
top level: BBa_J04480
start: 1046
end: 1086
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1537389
display id: annotation1537389
version: 1
top level: BBa_J04480
location: https://synbiohub.org/public/igem/BBa_J04480/annotation1537389/range1537389/1
component: BBa_B0010

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type: Range
persistent identity: range1537389
display id: range1537389
version: 1
direction: direction/forward
top level: BBa_J04480
start: 958
end: 1037
orientation: inline

BBa_E0030
type: SequenceAnnotation
persistent identity: annotation1537384
display id: annotation1537384
version: 1
top level: BBa_J04480
location: https://synbiohub.org/public/igem/BBa_J04480/annotation1537384/range1537384/1
component: BBa_E0030

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type: Range
persistent identity: range1537384
display id: range1537384
version: 1
direction: direction/forward
top level: BBa_J04480
start: 227
end: 949
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation1537372
display id: annotation1537372
version: 1
top level: BBa_J04480
location: https://synbiohub.org/public/igem/BBa_J04480/annotation1537372/range1537372/1
component: BBa_R0010

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type: Range
persistent identity: range1537372
display id: range1537372
version: 1
direction: direction/forward
top level: BBa_J04480
start: 1
end: 200
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1537382
display id: annotation1537382
version: 1
top level: BBa_J04480
location: https://synbiohub.org/public/igem/BBa_J04480/annotation1537382/range1537382/1
component: BBa_B0034

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type: Range
persistent identity: range1537382
display id: range1537382
version: 1
direction: direction/forward
top level: BBa_J04480
start: 209
end: 220
orientation: inline

BBa_I12057
type: ComponentDefinition
persistent identity: BBa I12057
display id: BBa_I12057
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: A protein (lambda cI) subassembly for modified BL oscillator based on I12040
creator: endelman
created: 2004-09-02T11:00:00Z
modified: 2015-08-31T04:07:31Z
discontinued: false
dominant: false
experience: experience/None
group u list: _3_
m user id: 0
owner id: 145
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This part produces POPS output to connect with a reporter.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_C0051
component: BBa_B0034
component: BBa_I12040
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_I12040
sequence annotation: BBa_B0010
sequence annotation: BBa_B0034
sequence annotation: BBa_C0051
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_I12057_sequence/1

BBa_C0051
type: Component
persistent identity: component1241693
display id: component1241693
version: 1
top level: BBa_I12057
access: public
definition: https://synbiohub.org/public/igem/BBa_C0051/1

BBa_B0034
type: Component
persistent identity: component1241683
display id: component1241683
version: 1
top level: BBa_I12057
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_I12040
type: Component
persistent identity: component1241678
display id: component1241678
version: 1
top level: BBa_I12057
access: public
definition: https://synbiohub.org/public/igem/BBa_I12040/1

BBa_B0012
type: Component
persistent identity: component1241709
display id: component1241709
version: 1
top level: BBa_I12057
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0010
type: Component
persistent identity: component1241699
display id: component1241699
version: 1
top level: BBa_I12057
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_I12040
type: Component
persistent identity: component1241735
display id: component1241735
version: 1
top level: BBa_I12057
access: public
definition: https://synbiohub.org/public/igem/BBa_I12040/1

BBa_I12040
type: SequenceAnnotation
persistent identity: annotation1241678
display id: annotation1241678
version: 1
top level: BBa_I12057
location: https://synbiohub.org/public/igem/BBa_I12057/annotation1241678/range1241678/1
component: BBa_I12040

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type: Range
persistent identity: range1241678
display id: range1241678
version: 1
direction: direction/forward
top level: BBa_I12057
start: 1
end: 91
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1241699
display id: annotation1241699
version: 1
top level: BBa_I12057
location: https://synbiohub.org/public/igem/BBa_I12057/annotation1241699/range1241699/1
component: BBa_B0010

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type: Range
persistent identity: range1241699
display id: range1241699
version: 1
direction: direction/forward
top level: BBa_I12057
start: 901
end: 980
orientation: inline

BBa_I12040
type: SequenceAnnotation
persistent identity: annotation1241735
display id: annotation1241735
version: 1
top level: BBa_I12057
location: https://synbiohub.org/public/igem/BBa_I12057/annotation1241735/range1241735/1
component: BBa_I12040

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type: Range
persistent identity: range1241735
display id: range1241735
version: 1
direction: direction/forward
top level: BBa_I12057
start: 1038
end: 1128
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1241683
display id: annotation1241683
version: 1
top level: BBa_I12057
location: https://synbiohub.org/public/igem/BBa_I12057/annotation1241683/range1241683/1
component: BBa_B0034

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type: Range
persistent identity: range1241683
display id: range1241683
version: 1
direction: direction/forward
top level: BBa_I12057
start: 100
end: 111
orientation: inline

BBa_C0051
type: SequenceAnnotation
persistent identity: annotation1241693
display id: annotation1241693
version: 1
top level: BBa_I12057
location: https://synbiohub.org/public/igem/BBa_I12057/annotation1241693/range1241693/1
component: BBa_C0051

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type: Range
persistent identity: range1241693
display id: range1241693
version: 1
direction: direction/forward
top level: BBa_I12057
start: 118
end: 867
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1241709
display id: annotation1241709
version: 1
top level: BBa_I12057
location: https://synbiohub.org/public/igem/BBa_I12057/annotation1241709/range1241709/1
component: BBa_B0012

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type: Range
persistent identity: range1241709
display id: range1241709
version: 1
direction: direction/forward
top level: BBa_I12057
start: 989
end: 1029
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BBa_J13004
type: ComponentDefinition
persistent identity: BBa J13004
display id: BBa_J13004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: polycistronic CFP/YFP expression cassette
creator: Jeff Tabor
created: 2005-06-27T11:00:00Z
modified: 2015-08-31T04:08:29Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _37_
m user id: 0
owner id: 88
owning group id: 37
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: A single mRNA containing CFP and YFP under the strong RBS B0034 is driven by the tet repressible promoter, R0040.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0034
component: BBa_E0020
component: BBa_B0010
component: BBa_R0040
component: BBa_E0030
component: BBa_B0012
sequence annotation: BBa_B0012
sequence annotation: BBa_R0040
sequence annotation: BBa_B0010
sequence annotation: BBa_B0034
sequence annotation: BBa_E0030
sequence annotation: BBa_E0020
sequence: https://synbiohub.org/public/igem/BBa_J13004_sequence/1

BBa_B0034
type: Component
persistent identity: component1555674
display id: component1555674
version: 1
top level: BBa_J13004
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_E0020
type: Component
persistent identity: component1555676
display id: component1555676
version: 1
top level: BBa_J13004
access: public
definition: https://synbiohub.org/public/igem/BBa_E0020/1

BBa_B0010
type: Component
persistent identity: component1555688
display id: component1555688
version: 1
top level: BBa_J13004
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_R0040
type: Component
persistent identity: component1555666
display id: component1555666
version: 1
top level: BBa_J13004
access: public
definition: https://synbiohub.org/public/igem/BBa_R0040/1

BBa_E0030
type: Component
persistent identity: component1555683
display id: component1555683
version: 1
top level: BBa_J13004
access: public
definition: https://synbiohub.org/public/igem/BBa_E0030/1

BBa_B0012
type: Component
persistent identity: component1555698
display id: component1555698
version: 1
top level: BBa_J13004
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0034
type: Component
persistent identity: component1555681
display id: component1555681
version: 1
top level: BBa_J13004
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1555698
display id: annotation1555698
version: 1
top level: BBa_J13004
location: https://synbiohub.org/public/igem/BBa_J13004/annotation1555698/range1555698/1
component: BBa_B0012

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type: Range
persistent identity: range1555698
display id: range1555698
version: 1
direction: direction/forward
top level: BBa_J13004
start: 1649
end: 1689
orientation: inline

BBa_R0040
type: SequenceAnnotation
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display id: annotation1555666
version: 1
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component: BBa_R0040

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type: Range
persistent identity: range1555666
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start: 1
end: 54
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1555688
display id: annotation1555688
version: 1
top level: BBa_J13004
location: https://synbiohub.org/public/igem/BBa_J13004/annotation1555688/range1555688/1
component: BBa_B0010

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type: Range
persistent identity: range1555688
display id: range1555688
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direction: direction/forward
top level: BBa_J13004
start: 1561
end: 1640
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1555681
display id: annotation1555681
version: 1
top level: BBa_J13004
location: https://synbiohub.org/public/igem/BBa_J13004/annotation1555681/range1555681/1
component: BBa_B0034

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persistent identity: range1555681
display id: range1555681
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start: 812
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BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1555674
display id: annotation1555674
version: 1
top level: BBa_J13004
location: https://synbiohub.org/public/igem/BBa_J13004/annotation1555674/range1555674/1
component: BBa_B0034

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type: Range
persistent identity: range1555674
display id: range1555674
version: 1
direction: direction/forward
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start: 63
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BBa_E0030
type: SequenceAnnotation
persistent identity: annotation1555683
display id: annotation1555683
version: 1
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location: https://synbiohub.org/public/igem/BBa_J13004/annotation1555683/range1555683/1
component: BBa_E0030

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type: Range
persistent identity: range1555683
display id: range1555683
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direction: direction/forward
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start: 830
end: 1552
orientation: inline

BBa_E0020
type: SequenceAnnotation
persistent identity: annotation1555676
display id: annotation1555676
version: 1
top level: BBa_J13004
location: https://synbiohub.org/public/igem/BBa_J13004/annotation1555676/range1555676/1
component: BBa_E0020

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type: Range
persistent identity: range1555676
display id: range1555676
version: 1
direction: direction/forward
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start: 81
end: 803
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BBa_I13921
type: ComponentDefinition
persistent identity: BBa I13921
display id: BBa_I13921
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Ring Oscillator Synchronization Device (I13260.Q04400.I0461)
creator: robertb
created: 2004-08-15T11:00:00Z
modified: 2015-08-31T04:07:37Z
discontinued: false
dominant: false
experience: experience/None
group u list: _6_
m user id: 0
owner id: 159
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is a device for synchronizing ring oscillators, based on research by Elowitz.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0010
component: BBa_R0062
component: BBa_C0061
component: BBa_B0012
component: BBa_C0040
component: BBa_B0034
component: BBa_R0063
component: BBa_C0062
component: BBa_R0040
sequence annotation: BBa_B0034
sequence annotation: BBa_R0040
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_C0062
sequence annotation: BBa_R0063
sequence annotation: BBa_R0062
sequence annotation: BBa_C0040
sequence annotation: BBa_C0061
sequence: https://synbiohub.org/public/igem/BBa_I13921_sequence/1

BBa_B0010
type: Component
persistent identity: component1055700
display id: component1055700
version: 1
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access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0010
type: Component
persistent identity: component1055750
display id: component1055750
version: 1
top level: BBa_I13921
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0010
type: Component
persistent identity: component1055651
display id: component1055651
version: 1
top level: BBa_I13921
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_R0062
type: Component
persistent identity: component1055676
display id: component1055676
version: 1
top level: BBa_I13921
access: public
definition: https://synbiohub.org/public/igem/BBa_R0062/1

BBa_C0061
type: Component
persistent identity: component1055743
display id: component1055743
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top level: BBa_I13921
access: public
definition: https://synbiohub.org/public/igem/BBa_C0061/1

BBa_B0012
type: Component
persistent identity: component1055710
display id: component1055710
version: 1
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access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_C0040
type: Component
persistent identity: component1055694
display id: component1055694
version: 1
top level: BBa_I13921
access: public
definition: https://synbiohub.org/public/igem/BBa_C0040/1

BBa_B0034
type: Component
persistent identity: component1055733
display id: component1055733
version: 1
top level: BBa_I13921
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_R0063
type: Component
persistent identity: component1055619
display id: component1055619
version: 1
top level: BBa_I13921
access: public
definition: https://synbiohub.org/public/igem/BBa_R0063/1

BBa_B0034
type: Component
persistent identity: component1055684
display id: component1055684
version: 1
top level: BBa_I13921
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_C0062
type: Component
persistent identity: component1055643
display id: component1055643
version: 1
top level: BBa_I13921
access: public
definition: https://synbiohub.org/public/igem/BBa_C0062/1

BBa_B0034
type: Component
persistent identity: component1055628
display id: component1055628
version: 1
top level: BBa_I13921
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0012
type: Component
persistent identity: component1055661
display id: component1055661
version: 1
top level: BBa_I13921
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0012
type: Component
persistent identity: component1055760
display id: component1055760
version: 1
top level: BBa_I13921
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_R0040
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persistent identity: component1055725
display id: component1055725
version: 1
top level: BBa_I13921
access: public
definition: https://synbiohub.org/public/igem/BBa_R0040/1

BBa_B0034
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type: Range
persistent identity: range1055628
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version: 1
direction: direction/forward
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start: 160
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orientation: inline

BBa_R0040
type: SequenceAnnotation
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display id: annotation1055725
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top level: BBa_I13921
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component: BBa_R0040

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type: Range
persistent identity: range1055725
display id: range1055725
version: 1
direction: direction/forward
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end: 2068
orientation: inline

BBa_B0010
type: SequenceAnnotation
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version: 1
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type: Range
persistent identity: range1055700
display id: range1055700
version: 1
direction: direction/forward
top level: BBa_I13921
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end: 1957
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1055750
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version: 1
top level: BBa_I13921
location: https://synbiohub.org/public/igem/BBa_I13921/annotation1055750/range1055750/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I13921/annotation1055750/range1055750/1
type: Range
persistent identity: range1055750
display id: range1055750
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direction: direction/forward
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orientation: inline

BBa_B0012
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display id: annotation1055710
version: 1
top level: BBa_I13921
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component: BBa_B0012

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type: Range
persistent identity: range1055710
display id: range1055710
version: 1
direction: direction/forward
top level: BBa_I13921
start: 1966
end: 2006
orientation: inline

BBa_C0062
type: SequenceAnnotation
persistent identity: annotation1055643
display id: annotation1055643
version: 1
top level: BBa_I13921
location: https://synbiohub.org/public/igem/BBa_I13921/annotation1055643/range1055643/1
component: BBa_C0062

https://synbiohub.org/public/igem/BBa_I13921/annotation1055643/range1055643/1
type: Range
persistent identity: range1055643
display id: range1055643
version: 1
direction: direction/forward
top level: BBa_I13921
start: 178
end: 933
orientation: inline

BBa_R0063
type: SequenceAnnotation
persistent identity: annotation1055619
display id: annotation1055619
version: 1
top level: BBa_I13921
location: https://synbiohub.org/public/igem/BBa_I13921/annotation1055619/range1055619/1
component: BBa_R0063

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type: Range
persistent identity: range1055619
display id: range1055619
version: 1
direction: direction/forward
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start: 1
end: 151
orientation: inline

BBa_R0062
type: SequenceAnnotation
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display id: annotation1055676
version: 1
top level: BBa_I13921
location: https://synbiohub.org/public/igem/BBa_I13921/annotation1055676/range1055676/1
component: BBa_R0062

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type: Range
persistent identity: range1055676
display id: range1055676
version: 1
direction: direction/forward
top level: BBa_I13921
start: 1104
end: 1158
orientation: inline

BBa_C0040
type: SequenceAnnotation
persistent identity: annotation1055694
display id: annotation1055694
version: 1
top level: BBa_I13921
location: https://synbiohub.org/public/igem/BBa_I13921/annotation1055694/range1055694/1
component: BBa_C0040

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type: Range
persistent identity: range1055694
display id: range1055694
version: 1
direction: direction/forward
top level: BBa_I13921
start: 1185
end: 1844
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1055661
display id: annotation1055661
version: 1
top level: BBa_I13921
location: https://synbiohub.org/public/igem/BBa_I13921/annotation1055661/range1055661/1
component: BBa_B0012

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type: Range
persistent identity: range1055661
display id: range1055661
version: 1
direction: direction/forward
top level: BBa_I13921
start: 1055
end: 1095
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1055733
display id: annotation1055733
version: 1
top level: BBa_I13921
location: https://synbiohub.org/public/igem/BBa_I13921/annotation1055733/range1055733/1
component: BBa_B0034

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type: Range
persistent identity: range1055733
display id: range1055733
version: 1
direction: direction/forward
top level: BBa_I13921
start: 2077
end: 2088
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1055760
display id: annotation1055760
version: 1
top level: BBa_I13921
location: https://synbiohub.org/public/igem/BBa_I13921/annotation1055760/range1055760/1
component: BBa_B0012

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type: Range
persistent identity: range1055760
display id: range1055760
version: 1
direction: direction/forward
top level: BBa_I13921
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end: 2874
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BBa_C0061
type: SequenceAnnotation
persistent identity: annotation1055743
display id: annotation1055743
version: 1
top level: BBa_I13921
location: https://synbiohub.org/public/igem/BBa_I13921/annotation1055743/range1055743/1
component: BBa_C0061

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type: Range
persistent identity: range1055743
display id: range1055743
version: 1
direction: direction/forward
top level: BBa_I13921
start: 2095
end: 2712
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1055684
display id: annotation1055684
version: 1
top level: BBa_I13921
location: https://synbiohub.org/public/igem/BBa_I13921/annotation1055684/range1055684/1
component: BBa_B0034

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type: Range
persistent identity: range1055684
display id: range1055684
version: 1
direction: direction/forward
top level: BBa_I13921
start: 1167
end: 1178
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1055651
display id: annotation1055651
version: 1
top level: BBa_I13921
location: https://synbiohub.org/public/igem/BBa_I13921/annotation1055651/range1055651/1
component: BBa_B0010

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type: Range
persistent identity: range1055651
display id: range1055651
version: 1
direction: direction/forward
top level: BBa_I13921
start: 967
end: 1046
orientation: inline

Kan ORF
type: ComponentDefinition
persistent identity: BBa I13801
display id: BBa_I13801
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Kanamycin Resistance ORF
creator: robertb
created: 2004-07-18T11:00:00Z
modified: 2015-08-31T04:07:36Z
discontinued: false
dominant: false
experience: experience/None
group u list: _6_
m user id: 0
owner id: 159
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is the wild-type cassette for kanamycin resistance.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: Kanamycin Resistance
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_I13801_sequence/1

Kanamycin Resistance
type: SequenceAnnotation
persistent identity: annotation902361
display id: annotation902361
version: 1
top level: Kan ORF
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I13801/annotation902361/range902361/1

https://synbiohub.org/public/igem/BBa_I13801/annotation902361/range902361/1
type: Range
persistent identity: range902361
display id: range902361
version: 1
direction: direction/forward
top level: Kan ORF
start: 1
end: 826
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2214012
display id: annotation2214012
version: 1
top level: Kan ORF
role: SO:0000807
role: feature/barcode
location: https://synbiohub.org/public/igem/BBa_I13801/annotation2214012/range2214012/1

https://synbiohub.org/public/igem/BBa_I13801/annotation2214012/range2214012/1
type: Range
persistent identity: range2214012
display id: range2214012
version: 1
direction: direction/forward
top level: Kan ORF
start: 817
end: 841
orientation: inline

BBa_Q01520
type: ComponentDefinition
persistent identity: BBa Q01520
display id: BBa_Q01520
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: QPI (B0033.C0052.B0015.R0052)
creator: Caitlin Conboy and Jennifer Braff
created: 2003-12-02T12:00:00Z
modified: 2015-05-08T01:14:13Z
discontinued: false
dominant: true
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_C0052
component: BBa_R0052
component: BBa_B0012
component: BBa_B0031
component: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_C0052
sequence annotation: BBa_B0010
sequence annotation: BBa_R0052
sequence annotation: BBa_B0031
sequence: https://synbiohub.org/public/igem/BBa_Q01520_sequence/1

BBa_C0052
type: Component
persistent identity: component951979
display id: component951979
version: 1
top level: BBa_Q01520
access: public
definition: https://synbiohub.org/public/igem/BBa_C0052/1

BBa_R0052
type: Component
persistent identity: component952018
display id: component952018
version: 1
top level: BBa_Q01520
access: public
definition: https://synbiohub.org/public/igem/BBa_R0052/1

BBa_B0012
type: Component
persistent identity: component951996
display id: component951996
version: 1
top level: BBa_Q01520
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0031
type: Component
persistent identity: component951969
display id: component951969
version: 1
top level: BBa_Q01520
access: public
definition: https://synbiohub.org/public/igem/BBa_B0031/1

BBa_B0010
type: Component
persistent identity: component951986
display id: component951986
version: 1
top level: BBa_Q01520
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation951996
display id: annotation951996
version: 1
top level: BBa_Q01520
location: https://synbiohub.org/public/igem/BBa_Q01520/annotation951996/range951996/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_Q01520/annotation951996/range951996/1
type: Range
persistent identity: range951996
display id: range951996
version: 1
direction: direction/forward
top level: BBa_Q01520
start: 811
end: 851
orientation: inline

BBa_C0052
type: SequenceAnnotation
persistent identity: annotation951979
display id: annotation951979
version: 1
top level: BBa_Q01520
location: https://synbiohub.org/public/igem/BBa_Q01520/annotation951979/range951979/1
component: BBa_C0052

https://synbiohub.org/public/igem/BBa_Q01520/annotation951979/range951979/1
type: Range
persistent identity: range951979
display id: range951979
version: 1
direction: direction/forward
top level: BBa_Q01520
start: 21
end: 689
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation951986
display id: annotation951986
version: 1
top level: BBa_Q01520
location: https://synbiohub.org/public/igem/BBa_Q01520/annotation951986/range951986/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_Q01520/annotation951986/range951986/1
type: Range
persistent identity: range951986
display id: range951986
version: 1
direction: direction/forward
top level: BBa_Q01520
start: 723
end: 802
orientation: inline

BBa_R0052
type: SequenceAnnotation
persistent identity: annotation952018
display id: annotation952018
version: 1
top level: BBa_Q01520
location: https://synbiohub.org/public/igem/BBa_Q01520/annotation952018/range952018/1
component: BBa_R0052

https://synbiohub.org/public/igem/BBa_Q01520/annotation952018/range952018/1
type: Range
persistent identity: range952018
display id: range952018
version: 1
direction: direction/forward
top level: BBa_Q01520
start: 860
end: 905
orientation: inline

BBa_B0031
type: SequenceAnnotation
persistent identity: annotation951969
display id: annotation951969
version: 1
top level: BBa_Q01520
location: https://synbiohub.org/public/igem/BBa_Q01520/annotation951969/range951969/1
component: BBa_B0031

https://synbiohub.org/public/igem/BBa_Q01520/annotation951969/range951969/1
type: Range
persistent identity: range951969
display id: range951969
version: 1
direction: direction/forward
top level: BBa_Q01520
start: 1
end: 14
orientation: inline

BBa_J05305
type: ComponentDefinition
persistent identity: BBa J05305
display id: BBa_J05305
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: R3 sys A -Terminator
creator: Alexander Roth
created: 2005-09-14T11:00:00Z
modified: 2015-08-31T04:08:15Z
discontinued: true
dominant: false
group u list: _34_
m user id: 0
owner id: 412
owning group id: 34
sample status: Discontinued
status: status/Deleted
bookmark: false
mutable description: R3 protein -Terminator (to be fused to e.g. reporter)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_J05101
component: BBa_B0034
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sequence annotation: BBa_B0034
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description: R3 sys A + Reporter
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modified: 2015-08-31T04:08:15Z
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description: Intermediate in two promoter screening plasmid
creator: Josh Michener
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modified: 2015-08-31T04:07:35Z
discontinued: false
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group u list: _11_
m user id: 0
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sample status: It's complicated
status: status/Planning
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mutable description: -- No description --
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BBa_B0034
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