//classic/generator/plasmids
type: Collection
persistent identity: classic generator plasmids
display id: classic_generator_plasmids
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
owned by: james
owned by: myers
member: BBa_K886003
member: BBa_K332033
member: BBa_K332032
member: BBa_K396011
member: ccdB
member: BBa_K332031
member: BBa_K886001
member: BBa_K886002

BBa_K332032
type: ComponentDefinition
persistent identity: BBa K332032
display id: BBa_K332032
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Constitutive promoter+37℃ induced RBS+tetR+double terminator+Ptet+RBS+GFP+double terminator
creator: KUO, CHIA-CHUN
created: 2010-10-21T11:00:00Z
modified: 2015-05-08T01:12:05Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _452_
m user id: 0
owner id: 7059
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: We want to utilize a temperature-regulated mechanism to control the expression period of a crystal protein. Therefore, we took advantage of the interaction between 37?? C induced RBS, tetR gene and pTet promoter to create the temperature regulating circuit. The following is a description of our prescribed strategy. First, we use the constitutive promoter, 37?? C induced RBS (K115002), the tetR gene and terminators. We will call this part the ???Regulator???.
mutable notes: none
mutable provenance: none
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_J61048
component: BBa_E0040
component: BBa_B0034
component: BBa_K332031
component: BBa_R0040
sequence annotation: BBa_R0040
sequence annotation: BBa_K332031
sequence annotation: BBa_B0034
sequence annotation: BBa_E0040
sequence annotation: BBa_J61048
sequence: https://synbiohub.org/public/igem/BBa_K332032_sequence/1

BBa_J61048
type: Component
persistent identity: component2273256
display id: component2273256
version: 1
top level: BBa_K332032
access: public
definition: https://synbiohub.org/public/igem/BBa_J61048/1

BBa_E0040
type: Component
persistent identity: component2273255
display id: component2273255
version: 1
top level: BBa_K332032
access: public
definition: https://synbiohub.org/public/igem/BBa_E0040/1

BBa_B0034
type: Component
persistent identity: component2273253
display id: component2273253
version: 1
top level: BBa_K332032
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K332031
type: Component
persistent identity: component2273246
display id: component2273246
version: 1
top level: BBa_K332032
access: public
definition: BBa_K332031

BBa_R0040
type: Component
persistent identity: component2273247
display id: component2273247
version: 1
top level: BBa_K332032
access: public
definition: https://synbiohub.org/public/igem/BBa_R0040/1

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation2273247
display id: annotation2273247
version: 1
top level: BBa_K332032
location: https://synbiohub.org/public/igem/BBa_K332032/annotation2273247/range2273247/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_K332032/annotation2273247/range2273247/1
type: Range
persistent identity: range2273247
display id: range2273247
version: 1
direction: direction/forward
top level: BBa_K332032
start: 916
end: 969
orientation: inline

BBa_K332031
type: SequenceAnnotation
persistent identity: annotation2273246
display id: annotation2273246
version: 1
top level: BBa_K332032
location: https://synbiohub.org/public/igem/BBa_K332032/annotation2273246/range2273246/1
component: BBa_K332031

https://synbiohub.org/public/igem/BBa_K332032/annotation2273246/range2273246/1
type: Range
persistent identity: range2273246
display id: range2273246
version: 1
direction: direction/reverse
top level: BBa_K332032
start: 1
end: 907
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2273253
display id: annotation2273253
version: 1
top level: BBa_K332032
location: https://synbiohub.org/public/igem/BBa_K332032/annotation2273253/range2273253/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K332032/annotation2273253/range2273253/1
type: Range
persistent identity: range2273253
display id: range2273253
version: 1
direction: direction/reverse
top level: BBa_K332032
start: 978
end: 989
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation2273255
display id: annotation2273255
version: 1
top level: BBa_K332032
location: https://synbiohub.org/public/igem/BBa_K332032/annotation2273255/range2273255/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_K332032/annotation2273255/range2273255/1
type: Range
persistent identity: range2273255
display id: range2273255
version: 1
direction: direction/reverse
top level: BBa_K332032
start: 996
end: 1715
orientation: inline

BBa_J61048
type: SequenceAnnotation
persistent identity: annotation2273256
display id: annotation2273256
version: 1
top level: BBa_K332032
location: https://synbiohub.org/public/igem/BBa_K332032/annotation2273256/range2273256/1
component: BBa_J61048

https://synbiohub.org/public/igem/BBa_K332032/annotation2273256/range2273256/1
type: Range
persistent identity: range2273256
display id: range2273256
version: 1
direction: direction/reverse
top level: BBa_K332032
start: 1724
end: 1836
orientation: inline

BBa_K886001
type: ComponentDefinition
persistent identity: BBa K886001
display id: BBa_K886001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Recombination Device composed by lox71-Cre recombinase
creator: edgar andres ochoa cruz
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:40Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _1149_
m user id: 0
owner id: 11298
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: This device was created to express the Cre recombinase enzyme and a target gene under the control of the same promoter (promoter not included in this part). The target gene needs to be amplified by PCR using primers that will insert a loxP and a lox66 sites flanking the sequence in order to integrate it in the lox71 recombination site located upstream the Cre recombinase gene. Once this device is located under the control of the desired promoter, it will express the Cre recombinase that recognizes the lox sites and inserts the target gene under the control of the same promoter. The Cre expression levels are critical for the recombination, we created two different devices: A (Cre-Lox71) and B (Lox71-Cre) for testing if inserting the target gene either upstream or downstream the Cre gene will affect the expression efficiency of the target gene.
mutable notes: We used the lox71 submitted by our group, which correct the part BBa_I718017 and BBa_K537020 when compared with the published sequence at http://www.ncbi.nlm.nih.gov/pmc/articles/PMC137435/
mutable provenance: We synthesized the lox71 site and then we assembled it with the Cre recombinase part BBa_J61047
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_K886000
component: BBa_J61047
sequence annotation: BBa_K886000
sequence annotation: BBa_J61047
sequence: https://synbiohub.org/public/igem/BBa_K886001_sequence/1

BBa_K886000
type: Component
persistent identity: component2196193
display id: component2196193
version: 1
top level: BBa_K886001
access: public
definition: https://synbiohub.org/public/igem/BBa_K886000/1

BBa_J61047
type: Component
persistent identity: component2196194
display id: component2196194
version: 1
top level: BBa_K886001
access: public
definition: https://synbiohub.org/public/igem/BBa_J61047/1

BBa_K886000
type: SequenceAnnotation
persistent identity: annotation2196193
display id: annotation2196193
version: 1
top level: BBa_K886001
location: https://synbiohub.org/public/igem/BBa_K886001/annotation2196193/range2196193/1
component: BBa_K886000

https://synbiohub.org/public/igem/BBa_K886001/annotation2196193/range2196193/1
type: Range
persistent identity: range2196193
display id: range2196193
version: 1
direction: direction/reverse
top level: BBa_K886001
start: 1
end: 34
orientation: inline

BBa_J61047
type: SequenceAnnotation
persistent identity: annotation2196194
display id: annotation2196194
version: 1
top level: BBa_K886001
location: https://synbiohub.org/public/igem/BBa_K886001/annotation2196194/range2196194/1
component: BBa_J61047

https://synbiohub.org/public/igem/BBa_K886001/annotation2196194/range2196194/1
type: Range
persistent identity: range2196194
display id: range2196194
version: 1
direction: direction/reverse
top level: BBa_K886001
start: 41
end: 1077
orientation: inline

BBa_K886003
type: ComponentDefinition
persistent identity: BBa K886003
display id: BBa_K886003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Recombination Device composed by Cre-lox71
creator: edgar andres ochoa cruz
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:40Z
discontinued: false
dominant: false
experience: experience/None
group u list: _940_
m user id: 0
owner id: 11298
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This device was created to express the Cre recombinase enzyme and a target gene under the control of the same promoter (promoter not included in this part). The target gene needs to be amplified by PCR, using primers that will insert a loxP and a lox66 sites flanking the sequence, in order to integrate it in the lox71 recombination site. For this part the lox71 is located downstream the Cre recombinase gene. An alternative construction is submitted in the part BBa_K886001. Once this device is located under the control of the desired promoter, it will express the Cre recombinase that recognizes the lox sites and inserts the target gene under the control of the same promoter. The Cre expression levels are critical for the recombination, we created two different devices: BBa_K886002 (Cre-Lox71) and BBa_K886001 (Lox71-Cre) for testing if inserting the target gene either upstream or downstream the Cre gene will affect the expression efficiency of the target gene
mutable notes: We used the lox71 submitted by our group BBa_K886000, which corrected the parts BBa_I718017 and BBa_K537020. Those parts are wrong when compared with the published sequence at http://www.ncbi.nlm.nih.gov/pmc/articles/PMC137435/
mutable provenance: We synthesized the lox71 site, then we assembled it downstream the Cre recombinase gene(BBa_J61047).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_K886000
component: BBa_J61047
sequence annotation: BBa_K886000
sequence annotation: BBa_J61047
sequence: https://synbiohub.org/public/igem/BBa_K886003_sequence/1

BBa_K886000
type: Component
persistent identity: component2196285
display id: component2196285
version: 1
top level: BBa_K886003
access: public
definition: https://synbiohub.org/public/igem/BBa_K886000/1

BBa_J61047
type: Component
persistent identity: component2196284
display id: component2196284
version: 1
top level: BBa_K886003
access: public
definition: https://synbiohub.org/public/igem/BBa_J61047/1

BBa_K886000
type: SequenceAnnotation
persistent identity: annotation2196285
display id: annotation2196285
version: 1
top level: BBa_K886003
location: https://synbiohub.org/public/igem/BBa_K886003/annotation2196285/range2196285/1
component: BBa_K886000

https://synbiohub.org/public/igem/BBa_K886003/annotation2196285/range2196285/1
type: Range
persistent identity: range2196285
display id: range2196285
version: 1
direction: direction/reverse
top level: BBa_K886003
start: 1046
end: 1079
orientation: inline

BBa_J61047
type: SequenceAnnotation
persistent identity: annotation2196284
display id: annotation2196284
version: 1
top level: BBa_K886003
location: https://synbiohub.org/public/igem/BBa_K886003/annotation2196284/range2196284/1
component: BBa_J61047

https://synbiohub.org/public/igem/BBa_K886003/annotation2196284/range2196284/1
type: Range
persistent identity: range2196284
display id: range2196284
version: 1
direction: direction/reverse
top level: BBa_K886003
start: 1
end: 1037
orientation: inline

BBa_K332033
type: ComponentDefinition
persistent identity: BBa K332033
display id: BBa_K332033
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: 37℃ induced RBS + tetR + double terminator
creator: KUO, CHIA-CHUN
created: 2010-10-23T11:00:00Z
modified: 2015-05-08T01:12:05Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _452_
m user id: 0
owner id: 7059
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: It is a transcriptional unit including 37℃ induced RBS + tetR + double terminator. It can be added various promoter to adapt your conditions.
mutable notes: To regulate following genes by temperature. To maintain cells at 37?? C ↑ : the tetR protein be generated and repress Ptet's circuit. To release cells at 37?? C ↓ : the tetR protein not be generated and do not repress Ptet's circuit.
mutable provenance: the 37℃ induced RBS cames from iGEM08_TUDelft
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_K115002
component: BBa_C0040
component: BBa_J61048
sequence annotation: BBa_K115002
sequence annotation: BBa_C0040
sequence annotation: BBa_J61048
sequence: https://synbiohub.org/public/igem/BBa_K332033_sequence/1

BBa_K115002
type: Component
persistent identity: component2247611
display id: component2247611
version: 1
top level: BBa_K332033
access: public
definition: https://synbiohub.org/public/igem/BBa_K115002/1

BBa_C0040
type: Component
persistent identity: component2247615
display id: component2247615
version: 1
top level: BBa_K332033
access: public
definition: https://synbiohub.org/public/igem/BBa_C0040/1

BBa_J61048
type: Component
persistent identity: component2247616
display id: component2247616
version: 1
top level: BBa_K332033
access: public
definition: https://synbiohub.org/public/igem/BBa_J61048/1

BBa_K115002
type: SequenceAnnotation
persistent identity: annotation2247611
display id: annotation2247611
version: 1
top level: BBa_K332033
location: https://synbiohub.org/public/igem/BBa_K332033/annotation2247611/range2247611/1
component: BBa_K115002

https://synbiohub.org/public/igem/BBa_K332033/annotation2247611/range2247611/1
type: Range
persistent identity: range2247611
display id: range2247611
version: 1
direction: direction/reverse
top level: BBa_K332033
start: 1
end: 52
orientation: inline

BBa_C0040
type: SequenceAnnotation
persistent identity: annotation2247615
display id: annotation2247615
version: 1
top level: BBa_K332033
location: https://synbiohub.org/public/igem/BBa_K332033/annotation2247615/range2247615/1
component: BBa_C0040

https://synbiohub.org/public/igem/BBa_K332033/annotation2247615/range2247615/1
type: Range
persistent identity: range2247615
display id: range2247615
version: 1
direction: direction/reverse
top level: BBa_K332033
start: 59
end: 743
orientation: inline

BBa_J61048
type: SequenceAnnotation
persistent identity: annotation2247616
display id: annotation2247616
version: 1
top level: BBa_K332033
location: https://synbiohub.org/public/igem/BBa_K332033/annotation2247616/range2247616/1
component: BBa_J61048

https://synbiohub.org/public/igem/BBa_K332033/annotation2247616/range2247616/1
type: Range
persistent identity: range2247616
display id: range2247616
version: 1
direction: direction/reverse
top level: BBa_K332033
start: 752
end: 864
orientation: inline

ccdB
type: ComponentDefinition
persistent identity: BBa P1016
display id: BBa_P1016
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: ccdB cassette without ccdA-
creator: Reshma Shetty
created: 2007-01-29T12:00:00Z
modified: 2015-06-15T01:13:47Z
discontinued: true
dominant: false
experience: experience/Works
group u list: _41_
m user id: 4206
owner id: 126
owning group id: 70
sample status: Not in stock
status: status/Deleted
bookmark: false
mutable description: This part is a differs from BBa_P1011 in that the mutations introduced in BBa_P1011 to remove restriction sites are not present in this part. It does have the "normal" ccd promoter and ccdB coding region. It also has a double TAA stop codon as per BioBrick conventions.
mutable notes: ccdA- was eliminated since it is an inactive form of ccdA and should not be required for the toxic phenotype of ccdB.
mutable provenance: This part is derived from BBa_P1010 but it lacks ccdA-.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: -35
sequence annotation: -10
sequence annotation: ccdB
sequence: https://synbiohub.org/public/igem/BBa_P1016_sequence/1

-35
type: SequenceAnnotation
persistent identity: annotation1910684
display id: annotation1910684
version: 1
top level: ccdB
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_P1016/annotation1910684/range1910684/1

https://synbiohub.org/public/igem/BBa_P1016/annotation1910684/range1910684/1
type: Range
persistent identity: range1910684
display id: range1910684
version: 1
direction: direction/forward
top level: ccdB
start: 41
end: 46
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1910685
display id: annotation1910685
version: 1
top level: ccdB
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_P1016/annotation1910685/range1910685/1

https://synbiohub.org/public/igem/BBa_P1016/annotation1910685/range1910685/1
type: Range
persistent identity: range1910685
display id: range1910685
version: 1
direction: direction/forward
top level: ccdB
start: 64
end: 69
orientation: inline

ccdB
type: SequenceAnnotation
persistent identity: annotation1910686
display id: annotation1910686
version: 1
top level: ccdB
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_P1016/annotation1910686/range1910686/1

https://synbiohub.org/public/igem/BBa_P1016/annotation1910686/range1910686/1
type: Range
persistent identity: range1910686
display id: range1910686
version: 1
direction: direction/forward
top level: ccdB
start: 108
end: 416
orientation: inline

BBa_K886002
type: ComponentDefinition
persistent identity: BBa K886002
display id: BBa_K886002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Recombination Device composed by Cre-lox71
creator: edgar andres ochoa cruz
created: 2012-09-23T11:00:00Z
is replaced by: BBa_K886001
modified: 2015-05-08T01:13:40Z
discontinued: true
dominant: false
experience: experience/None
group u list: _1149_
m user id: 0
owner id: 11298
owning group id: 9
sample status: Discontinued
status: status/Deleted
bookmark: false
mutable description: one
mutable notes: None
mutable provenance: composite
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_K886001
sequence annotation: BBa_K886001
sequence: https://synbiohub.org/public/igem/BBa_K886002_sequence/1

BBa_K886001
type: Component
persistent identity: component2196198
display id: component2196198
version: 1
top level: BBa_K886002
access: public
definition: BBa_K886001

BBa_K886001
type: SequenceAnnotation
persistent identity: annotation2196198
display id: annotation2196198
version: 1
top level: BBa_K886002
location: https://synbiohub.org/public/igem/BBa_K886002/annotation2196198/range2196198/1
component: BBa_K886001

https://synbiohub.org/public/igem/BBa_K886002/annotation2196198/range2196198/1
type: Range
persistent identity: range2196198
display id: range2196198
version: 1
direction: direction/reverse
top level: BBa_K886002
start: 1
end: 1077
orientation: inline

BBa_K332031
type: ComponentDefinition
persistent identity: BBa K332031
display id: BBa_K332031
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Constitutive promoter + 37℃ induced RBS + tetR + double terminator
creator: KUO, CHIA-CHUN
created: 2010-10-21T11:00:00Z
modified: 2015-05-08T01:12:05Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _452_
m user id: 0
owner id: 7059
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: we took advantage of the interaction between 37?? C induced RBS, tetR gene and pTet promoter to create the temperature regulating circuit. The following is a description of our prescribed strategy. First, we use the constitutive promoter, 37?? C induced RBS (K115002), the tetR gene and terminators. We will call this part the ???Regulator???. We speculate that when the ???Regulator??? and ???Production??? sections are combined, we can achieve a high bacteria growth rate and close to no target protein producing rate at T>37??C, and low bacteria growth rate and high target protein producing rate at T<37??C.
mutable notes: To regulate following gene by temperature
mutable provenance: iGEM08_TUDelft
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_C0040
component: BBa_K115002
component: BBa_J61048
component: BBa_J23101
sequence annotation: BBa_C0040
sequence annotation: BBa_J61048
sequence annotation: BBa_J23101
sequence annotation: BBa_K115002
sequence: https://synbiohub.org/public/igem/BBa_K332031_sequence/1

BBa_C0040
type: Component
persistent identity: component2250406
display id: component2250406
version: 1
top level: BBa_K332031
access: public
definition: https://synbiohub.org/public/igem/BBa_C0040/1

BBa_K115002
type: Component
persistent identity: component2250402
display id: component2250402
version: 1
top level: BBa_K332031
access: public
definition: https://synbiohub.org/public/igem/BBa_K115002/1

BBa_J61048
type: Component
persistent identity: component2250407
display id: component2250407
version: 1
top level: BBa_K332031
access: public
definition: https://synbiohub.org/public/igem/BBa_J61048/1

BBa_J23101
type: Component
persistent identity: component2250397
display id: component2250397
version: 1
top level: BBa_K332031
access: public
definition: https://synbiohub.org/public/igem/BBa_J23101/1

BBa_C0040
type: SequenceAnnotation
persistent identity: annotation2250406
display id: annotation2250406
version: 1
top level: BBa_K332031
location: https://synbiohub.org/public/igem/BBa_K332031/annotation2250406/range2250406/1
component: BBa_C0040

https://synbiohub.org/public/igem/BBa_K332031/annotation2250406/range2250406/1
type: Range
persistent identity: range2250406
display id: range2250406
version: 1
direction: direction/reverse
top level: BBa_K332031
start: 102
end: 786
orientation: inline

BBa_J61048
type: SequenceAnnotation
persistent identity: annotation2250407
display id: annotation2250407
version: 1
top level: BBa_K332031
location: https://synbiohub.org/public/igem/BBa_K332031/annotation2250407/range2250407/1
component: BBa_J61048

https://synbiohub.org/public/igem/BBa_K332031/annotation2250407/range2250407/1
type: Range
persistent identity: range2250407
display id: range2250407
version: 1
direction: direction/reverse
top level: BBa_K332031
start: 795
end: 907
orientation: inline

BBa_J23101
type: SequenceAnnotation
persistent identity: annotation2250397
display id: annotation2250397
version: 1
top level: BBa_K332031
location: https://synbiohub.org/public/igem/BBa_K332031/annotation2250397/range2250397/1
component: BBa_J23101

https://synbiohub.org/public/igem/BBa_K332031/annotation2250397/range2250397/1
type: Range
persistent identity: range2250397
display id: range2250397
version: 1
direction: direction/reverse
top level: BBa_K332031
start: 1
end: 35
orientation: inline

BBa_K115002
type: SequenceAnnotation
persistent identity: annotation2250402
display id: annotation2250402
version: 1
top level: BBa_K332031
location: https://synbiohub.org/public/igem/BBa_K332031/annotation2250402/range2250402/1
component: BBa_K115002

https://synbiohub.org/public/igem/BBa_K332031/annotation2250402/range2250402/1
type: Range
persistent identity: range2250402
display id: range2250402
version: 1
direction: direction/reverse
top level: BBa_K332031
start: 44
end: 95
orientation: inline

BBa_K396011
type: ComponentDefinition
persistent identity: BBa K396011
display id: BBa_K396011
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LuxR protein generator
creator: Ike Kohei
created: 2010-10-23T11:00:00Z
modified: 2015-05-08T01:12:22Z
discontinued: false
dominant: false
group u list: _519_
m user id: 0
owner id: 7744
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: LuxR protein generator
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: BBa_B0034
sequence annotation: BBa_C0062
sequence annotation: BBa_J23100
sequence annotation: luxR
sequence: https://synbiohub.org/public/igem/BBa_K396011_sequence/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2109754
display id: annotation2109754
version: 1
top level: BBa_K396011
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K396011/annotation2109754/range2109754/1

https://synbiohub.org/public/igem/BBa_K396011/annotation2109754/range2109754/1
type: Range
persistent identity: range2109754
display id: range2109754
version: 1
direction: direction/forward
top level: BBa_K396011
start: 51
end: 61
orientation: inline

BBa_C0062
type: SequenceAnnotation
persistent identity: annotation2109755
display id: annotation2109755
version: 1
top level: BBa_K396011
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K396011/annotation2109755/range2109755/1

https://synbiohub.org/public/igem/BBa_K396011/annotation2109755/range2109755/1
type: Range
persistent identity: range2109755
display id: range2109755
version: 1
direction: direction/forward
top level: BBa_K396011
start: 68
end: 820
orientation: inline

BBa_J23100
type: SequenceAnnotation
persistent identity: annotation2109753
display id: annotation2109753
version: 1
top level: BBa_K396011
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K396011/annotation2109753/range2109753/1

https://synbiohub.org/public/igem/BBa_K396011/annotation2109753/range2109753/1
type: Range
persistent identity: range2109753
display id: range2109753
version: 1
direction: direction/forward
top level: BBa_K396011
start: 7
end: 41
orientation: inline

luxR
type: SequenceAnnotation
persistent identity: annotation2109756
display id: annotation2109756
version: 1
top level: BBa_K396011
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K396011/annotation2109756/range2109756/1

https://synbiohub.org/public/igem/BBa_K396011/annotation2109756/range2109756/1
type: Range
persistent identity: range2109756
display id: range2109756
version: 1
direction: direction/forward
top level: BBa_K396011
start: 68
end: 820
orientation: inline