//classic/generator/prc
type: Collection
persistent identity: classic generator prc
display id: classic_generator_prc
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
owned by: james
owned by: myers
member: BBa_K118020
member: BBa_K118019
member: BBa_K283027
member: LacI:SDnaK
member: pBAD:Cpn60
member: BBa_K283022
member: LacI:CspC
member: pBAD:CspA
member: Plac-sam8
member: BBa_K283024
member: pBAD:CspC
member: BBa_K283005
member: BBa_K499596
member: LacI:Cpn60
member: pBAD:Cpn10
member: BBa_K283001
member: BBa_K732002
member: LacI:CspA
member: pBAD:SDnaK
member: ninabGen
member: BBa_K283011
member: LacI:Cpn10
member: BBa_K283032

BBa_K118019
type: ComponentDefinition
persistent identity: BBa K118019
display id: BBa_K118019
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Plac+rbs+glgC (ADP-glucose pyrophosphorylase)
creator: Andrew Hall
created: 2008-10-05T11:00:00Z
modified: 2015-05-08T01:09:37Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _192_
m user id: 0
owner id: 3282
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: This is the coding sequence of glgC from ''Escherichia coli'' JM109 (see [http://partsregistry.org/wiki/index.php?title=Part:BBa_K118015 BBa_K118015]) with a ribosome binding site coupled to an upstream Lac promoter and ''lacZ''' as a method for testing expression.
mutable notes: No special considerations
mutable provenance: ''Escherichia coli'' JM109 genomic DNA.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K118015
component: BBa_J33207
component: BBa_J15001
sequence annotation: BBa_J33207
sequence annotation: BBa_J15001
sequence annotation: BBa_K118015
sequence: https://synbiohub.org/public/igem/BBa_K118019_sequence/1

BBa_K118015
type: Component
persistent identity: component1978666
display id: component1978666
version: 1
top level: BBa_K118019
access: public
definition: https://synbiohub.org/public/igem/BBa_K118015/1

BBa_J33207
type: Component
persistent identity: component1978660
display id: component1978660
version: 1
top level: BBa_K118019
access: public
definition: https://synbiohub.org/public/igem/BBa_J33207/1

BBa_J15001
type: Component
persistent identity: component1978663
display id: component1978663
version: 1
top level: BBa_K118019
access: public
definition: https://synbiohub.org/public/igem/BBa_J15001/1

BBa_J33207
type: SequenceAnnotation
persistent identity: annotation1978660
display id: annotation1978660
version: 1
top level: BBa_K118019
location: https://synbiohub.org/public/igem/BBa_K118019/annotation1978660/range1978660/1
component: BBa_J33207

https://synbiohub.org/public/igem/BBa_K118019/annotation1978660/range1978660/1
type: Range
persistent identity: range1978660
display id: range1978660
version: 1
direction: direction/reverse
top level: BBa_K118019
start: 1
end: 600
orientation: inline

BBa_J15001
type: SequenceAnnotation
persistent identity: annotation1978663
display id: annotation1978663
version: 1
top level: BBa_K118019
location: https://synbiohub.org/public/igem/BBa_K118019/annotation1978663/range1978663/1
component: BBa_J15001

https://synbiohub.org/public/igem/BBa_K118019/annotation1978663/range1978663/1
type: Range
persistent identity: range1978663
display id: range1978663
version: 1
direction: direction/reverse
top level: BBa_K118019
start: 609
end: 618
orientation: inline

BBa_K118015
type: SequenceAnnotation
persistent identity: annotation1978666
display id: annotation1978666
version: 1
top level: BBa_K118019
location: https://synbiohub.org/public/igem/BBa_K118019/annotation1978666/range1978666/1
component: BBa_K118015

https://synbiohub.org/public/igem/BBa_K118019/annotation1978666/range1978666/1
type: Range
persistent identity: range1978666
display id: range1978666
version: 1
direction: direction/reverse
top level: BBa_K118019
start: 625
end: 1923
orientation: inline

pBAD:SDnaK
type: ComponentDefinition
persistent identity: BBa K538302
display id: BBa_K538302
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pBAD + RBS + SheDnaK
creator: Bas Stringer
created: 2011-09-07T11:00:00Z
modified: 2015-05-08T01:12:37Z
discontinued: false
dominant: false
experience: experience/None
group u list: _704_
m user id: 0
owner id: 9985
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: -
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K206000
component: BBa_B0034
component: BBa_K538002
sequence annotation: BBa_K206000
sequence annotation: BBa_K538002
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K538302_sequence/1

BBa_K206000
type: Component
persistent identity: component2126777
display id: component2126777
version: 1
top level: pBAD:SDnaK
access: public
definition: https://synbiohub.org/public/igem/BBa_K206000/1

BBa_B0034
type: Component
persistent identity: component2126779
display id: component2126779
version: 1
top level: pBAD:SDnaK
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K538002
type: Component
persistent identity: component2126782
display id: component2126782
version: 1
top level: pBAD:SDnaK
access: public
definition: https://synbiohub.org/public/igem/BBa_K538002/1

BBa_K206000
type: SequenceAnnotation
persistent identity: annotation2126777
display id: annotation2126777
version: 1
top level: pBAD:SDnaK
location: https://synbiohub.org/public/igem/BBa_K538302/annotation2126777/range2126777/1
component: BBa_K206000

https://synbiohub.org/public/igem/BBa_K538302/annotation2126777/range2126777/1
type: Range
persistent identity: range2126777
display id: range2126777
version: 1
direction: direction/reverse
top level: pBAD:SDnaK
start: 1
end: 130
orientation: inline

BBa_K538002
type: SequenceAnnotation
persistent identity: annotation2126782
display id: annotation2126782
version: 1
top level: pBAD:SDnaK
location: https://synbiohub.org/public/igem/BBa_K538302/annotation2126782/range2126782/1
component: BBa_K538002

https://synbiohub.org/public/igem/BBa_K538302/annotation2126782/range2126782/1
type: Range
persistent identity: range2126782
display id: range2126782
version: 1
direction: direction/reverse
top level: pBAD:SDnaK
start: 157
end: 2076
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2126779
display id: annotation2126779
version: 1
top level: pBAD:SDnaK
location: https://synbiohub.org/public/igem/BBa_K538302/annotation2126779/range2126779/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K538302/annotation2126779/range2126779/1
type: Range
persistent identity: range2126779
display id: range2126779
version: 1
direction: direction/reverse
top level: pBAD:SDnaK
start: 139
end: 150
orientation: inline

BBa_K283027
type: ComponentDefinition
persistent identity: BBa K283027
display id: BBa_K283027
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: rbs-GFP(LVA)-terminators (Backbone: pSB1AK3)
creator: Nelson Chu, W Ling , Shi Lei
created: 2009-10-12T11:00:00Z
modified: 2015-05-08T01:11:47Z
discontinued: false
dominant: false
experience: experience/None
group u list: _383_
m user id: 0
owner id: 5494
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: The chez is responsible for the dephosphorylation of cheY protein in bacteria flagella movement and is tagged by a 6-histidine tail. The pLac promoter can be repressed by LacI repressor protein
mutable notes: BBa_K283027
mutable provenance: The chez-histag coding region is self-designed The pLac promoter , rbs are from the registry
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0012
component: BBa_J04031
component: BBa_B0010
component: BBa_B0034
sequence annotation: BBa_J04031
sequence annotation: BBa_B0010
sequence annotation: BBa_B0034
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_K283027_sequence/1

BBa_B0012
type: Component
persistent identity: component2056902
display id: component2056902
version: 1
top level: BBa_K283027
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_J04031
type: Component
persistent identity: component2056899
display id: component2056899
version: 1
top level: BBa_K283027
access: public
definition: https://synbiohub.org/public/igem/BBa_J04031/1

BBa_B0010
type: Component
persistent identity: component2056900
display id: component2056900
version: 1
top level: BBa_K283027
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0034
type: Component
persistent identity: component2056894
display id: component2056894
version: 1
top level: BBa_K283027
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_J04031
type: SequenceAnnotation
persistent identity: annotation2056899
display id: annotation2056899
version: 1
top level: BBa_K283027
location: https://synbiohub.org/public/igem/BBa_K283027/annotation2056899/range2056899/1
component: BBa_J04031

https://synbiohub.org/public/igem/BBa_K283027/annotation2056899/range2056899/1
type: Range
persistent identity: range2056899
display id: range2056899
version: 1
direction: direction/reverse
top level: BBa_K283027
start: 19
end: 777
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2056900
display id: annotation2056900
version: 1
top level: BBa_K283027
location: https://synbiohub.org/public/igem/BBa_K283027/annotation2056900/range2056900/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K283027/annotation2056900/range2056900/1
type: Range
persistent identity: range2056900
display id: range2056900
version: 1
direction: direction/forward
top level: BBa_K283027
start: 786
end: 865
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2056894
display id: annotation2056894
version: 1
top level: BBa_K283027
location: https://synbiohub.org/public/igem/BBa_K283027/annotation2056894/range2056894/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K283027/annotation2056894/range2056894/1
type: Range
persistent identity: range2056894
display id: range2056894
version: 1
direction: direction/reverse
top level: BBa_K283027
start: 1
end: 12
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2056902
display id: annotation2056902
version: 1
top level: BBa_K283027
location: https://synbiohub.org/public/igem/BBa_K283027/annotation2056902/range2056902/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K283027/annotation2056902/range2056902/1
type: Range
persistent identity: range2056902
display id: range2056902
version: 1
direction: direction/forward
top level: BBa_K283027
start: 874
end: 914
orientation: inline

BBa_K118020
type: ComponentDefinition
persistent identity: BBa K118020
display id: BBa_K118020
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Plac+rbs+glgC16 (glgC with G336D substitution))
creator: Andrew Hall
created: 2008-10-05T11:00:00Z
modified: 2015-05-08T01:09:37Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _192_
m user id: 0
owner id: 3282
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: This is the coding sequence of ''glgC''16 from ''Escherichia coli'' JM109 (see [http://partsregistry.org/wiki/index.php?title=Part:BBa_K118016 BBa_K118016]) with a ribosome binding site coupled to an upstream Lac promoter and ''lacZ''' as a method for testing expression.
mutable notes: No special considerations
mutable provenance: ''Escherichia coli'' JM109 genomic DNA.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K118016
component: BBa_J15001
component: BBa_J33207
sequence annotation: BBa_K118016
sequence annotation: BBa_J33207
sequence annotation: BBa_J15001
sequence: https://synbiohub.org/public/igem/BBa_K118020_sequence/1

BBa_K118016
type: Component
persistent identity: component1978652
display id: component1978652
version: 1
top level: BBa_K118020
access: public
definition: https://synbiohub.org/public/igem/BBa_K118016/1

BBa_J15001
type: Component
persistent identity: component1978648
display id: component1978648
version: 1
top level: BBa_K118020
access: public
definition: https://synbiohub.org/public/igem/BBa_J15001/1

BBa_J33207
type: Component
persistent identity: component1978645
display id: component1978645
version: 1
top level: BBa_K118020
access: public
definition: https://synbiohub.org/public/igem/BBa_J33207/1

BBa_K118016
type: SequenceAnnotation
persistent identity: annotation1978652
display id: annotation1978652
version: 1
top level: BBa_K118020
location: https://synbiohub.org/public/igem/BBa_K118020/annotation1978652/range1978652/1
component: BBa_K118016

https://synbiohub.org/public/igem/BBa_K118020/annotation1978652/range1978652/1
type: Range
persistent identity: range1978652
display id: range1978652
version: 1
direction: direction/reverse
top level: BBa_K118020
start: 625
end: 1923
orientation: inline

BBa_J33207
type: SequenceAnnotation
persistent identity: annotation1978645
display id: annotation1978645
version: 1
top level: BBa_K118020
location: https://synbiohub.org/public/igem/BBa_K118020/annotation1978645/range1978645/1
component: BBa_J33207

https://synbiohub.org/public/igem/BBa_K118020/annotation1978645/range1978645/1
type: Range
persistent identity: range1978645
display id: range1978645
version: 1
direction: direction/reverse
top level: BBa_K118020
start: 1
end: 600
orientation: inline

BBa_J15001
type: SequenceAnnotation
persistent identity: annotation1978648
display id: annotation1978648
version: 1
top level: BBa_K118020
location: https://synbiohub.org/public/igem/BBa_K118020/annotation1978648/range1978648/1
component: BBa_J15001

https://synbiohub.org/public/igem/BBa_K118020/annotation1978648/range1978648/1
type: Range
persistent identity: range1978648
display id: range1978648
version: 1
direction: direction/reverse
top level: BBa_K118020
start: 609
end: 618
orientation: inline

BBa_K283032
type: ComponentDefinition
persistent identity: BBa K283032
display id: BBa_K283032
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pTet-rbs-cI(lambda LVA)
creator: Nelson Chu, W Ling , Shi Lei
created: 2009-10-15T11:00:00Z
modified: 2015-05-08T01:11:47Z
discontinued: false
dominant: false
experience: experience/None
group u list: _383_
m user id: 0
owner id: 5494
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: THe pTet promoter can be repressed by TetR repressor protein. The cI protein generated can repress plambda(R-O12)(K094002) promoter. This part lacks a terminator
mutable notes: BBa_K283032
mutable provenance: The pTet promoter and the rbs comes from the registry, The cI protein comes from lambda phage
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_C0051
component: BBa_B0034
component: BBa_R0040
sequence annotation: BBa_R0040
sequence annotation: BBa_C0051
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K283032_sequence/1

BBa_C0051
type: Component
persistent identity: component2246766
display id: component2246766
version: 1
top level: BBa_K283032
access: public
definition: https://synbiohub.org/public/igem/BBa_C0051/1

BBa_B0034
type: Component
persistent identity: component2246762
display id: component2246762
version: 1
top level: BBa_K283032
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_R0040
type: Component
persistent identity: component2246756
display id: component2246756
version: 1
top level: BBa_K283032
access: public
definition: https://synbiohub.org/public/igem/BBa_R0040/1

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation2246756
display id: annotation2246756
version: 1
top level: BBa_K283032
location: https://synbiohub.org/public/igem/BBa_K283032/annotation2246756/range2246756/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_K283032/annotation2246756/range2246756/1
type: Range
persistent identity: range2246756
display id: range2246756
version: 1
direction: direction/forward
top level: BBa_K283032
start: 1
end: 54
orientation: inline

BBa_C0051
type: SequenceAnnotation
persistent identity: annotation2246766
display id: annotation2246766
version: 1
top level: BBa_K283032
location: https://synbiohub.org/public/igem/BBa_K283032/annotation2246766/range2246766/1
component: BBa_C0051

https://synbiohub.org/public/igem/BBa_K283032/annotation2246766/range2246766/1
type: Range
persistent identity: range2246766
display id: range2246766
version: 1
direction: direction/reverse
top level: BBa_K283032
start: 81
end: 855
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2246762
display id: annotation2246762
version: 1
top level: BBa_K283032
location: https://synbiohub.org/public/igem/BBa_K283032/annotation2246762/range2246762/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K283032/annotation2246762/range2246762/1
type: Range
persistent identity: range2246762
display id: range2246762
version: 1
direction: direction/reverse
top level: BBa_K283032
start: 63
end: 74
orientation: inline

BBa_K283001
type: ComponentDefinition
persistent identity: BBa K283001
display id: BBa_K283001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pT7-rbs-(sp_eGFP_streptavidin_AIDAc)-Terminators
creator: Chenli
created: 2009-10-12T11:00:00Z
modified: 2015-05-08T01:11:47Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _383_
m user id: 0
owner id: 5494
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: This part codes for part of the protein invisine. When fused with other protein, it can direct the protein to be expressed on the membrane
mutable notes: BBa_K283001
mutable provenance: self-designed
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_I712074
component: BBa_B0010
component: BBa_B0012
component: BBa_B0034
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_B0034
sequence annotation: BBa_I712074
sequence: https://synbiohub.org/public/igem/BBa_K283001_sequence/1

BBa_I712074
type: Component
persistent identity: component2055561
display id: component2055561
version: 1
top level: BBa_K283001
access: public
definition: https://synbiohub.org/public/igem/BBa_I712074/1

BBa_B0010
type: Component
persistent identity: component2055566
display id: component2055566
version: 1
top level: BBa_K283001
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0012
type: Component
persistent identity: component2055568
display id: component2055568
version: 1
top level: BBa_K283001
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0034
type: Component
persistent identity: component2055563
display id: component2055563
version: 1
top level: BBa_K283001
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2055566
display id: annotation2055566
version: 1
top level: BBa_K283001
location: https://synbiohub.org/public/igem/BBa_K283001/annotation2055566/range2055566/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K283001/annotation2055566/range2055566/1
type: Range
persistent identity: range2055566
display id: range2055566
version: 1
direction: direction/forward
top level: BBa_K283001
start: 2868
end: 2947
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2055568
display id: annotation2055568
version: 1
top level: BBa_K283001
location: https://synbiohub.org/public/igem/BBa_K283001/annotation2055568/range2055568/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K283001/annotation2055568/range2055568/1
type: Range
persistent identity: range2055568
display id: range2055568
version: 1
direction: direction/forward
top level: BBa_K283001
start: 2956
end: 2996
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2055563
display id: annotation2055563
version: 1
top level: BBa_K283001
location: https://synbiohub.org/public/igem/BBa_K283001/annotation2055563/range2055563/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K283001/annotation2055563/range2055563/1
type: Range
persistent identity: range2055563
display id: range2055563
version: 1
direction: direction/reverse
top level: BBa_K283001
start: 55
end: 66
orientation: inline

BBa_I712074
type: SequenceAnnotation
persistent identity: annotation2055561
display id: annotation2055561
version: 1
top level: BBa_K283001
location: https://synbiohub.org/public/igem/BBa_K283001/annotation2055561/range2055561/1
component: BBa_I712074

https://synbiohub.org/public/igem/BBa_K283001/annotation2055561/range2055561/1
type: Range
persistent identity: range2055561
display id: range2055561
version: 1
direction: direction/reverse
top level: BBa_K283001
start: 1
end: 46
orientation: inline

pBAD:CspC
type: ComponentDefinition
persistent identity: BBa K538304
display id: BBa_K538304
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pBAD + RBS + CspC
creator: Bas Stringer
created: 2011-09-07T11:00:00Z
modified: 2015-05-08T01:12:37Z
discontinued: false
dominant: false
experience: experience/None
group u list: _704_
m user id: 0
owner id: 9985
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: -
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0034
component: BBa_K538004
component: BBa_K206000
sequence annotation: BBa_K206000
sequence annotation: BBa_B0034
sequence annotation: BBa_K538004
sequence: https://synbiohub.org/public/igem/BBa_K538304_sequence/1

BBa_B0034
type: Component
persistent identity: component2134103
display id: component2134103
version: 1
top level: pBAD:CspC
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K538004
type: Component
persistent identity: component2134109
display id: component2134109
version: 1
top level: pBAD:CspC
access: public
definition: https://synbiohub.org/public/igem/BBa_K538004/1

BBa_K206000
type: Component
persistent identity: component2134101
display id: component2134101
version: 1
top level: pBAD:CspC
access: public
definition: https://synbiohub.org/public/igem/BBa_K206000/1

BBa_K206000
type: SequenceAnnotation
persistent identity: annotation2134101
display id: annotation2134101
version: 1
top level: pBAD:CspC
location: https://synbiohub.org/public/igem/BBa_K538304/annotation2134101/range2134101/1
component: BBa_K206000

https://synbiohub.org/public/igem/BBa_K538304/annotation2134101/range2134101/1
type: Range
persistent identity: range2134101
display id: range2134101
version: 1
direction: direction/reverse
top level: pBAD:CspC
start: 1
end: 130
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2134103
display id: annotation2134103
version: 1
top level: pBAD:CspC
location: https://synbiohub.org/public/igem/BBa_K538304/annotation2134103/range2134103/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K538304/annotation2134103/range2134103/1
type: Range
persistent identity: range2134103
display id: range2134103
version: 1
direction: direction/reverse
top level: pBAD:CspC
start: 139
end: 150
orientation: inline

BBa_K538004
type: SequenceAnnotation
persistent identity: annotation2134109
display id: annotation2134109
version: 1
top level: pBAD:CspC
location: https://synbiohub.org/public/igem/BBa_K538304/annotation2134109/range2134109/1
component: BBa_K538004

https://synbiohub.org/public/igem/BBa_K538304/annotation2134109/range2134109/1
type: Range
persistent identity: range2134109
display id: range2134109
version: 1
direction: direction/reverse
top level: pBAD:CspC
start: 157
end: 351
orientation: inline

LacI:Cpn60
type: ComponentDefinition
persistent identity: BBa K538201
display id: BBa_K538201
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pLacI + RBS + Cpn60
creator: Bas Stringer
created: 2011-09-07T11:00:00Z
modified: 2015-05-08T01:12:37Z
discontinued: false
dominant: false
experience: experience/None
group u list: _704_
m user id: 0
owner id: 9985
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: -
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0034
component: BBa_K538001
component: BBa_R0010
sequence annotation: BBa_K538001
sequence annotation: BBa_B0034
sequence annotation: BBa_R0010
sequence: https://synbiohub.org/public/igem/BBa_K538201_sequence/1

BBa_B0034
type: Component
persistent identity: component2219873
display id: component2219873
version: 1
top level: LacI:Cpn60
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K538001
type: Component
persistent identity: component2219877
display id: component2219877
version: 1
top level: LacI:Cpn60
access: public
definition: https://synbiohub.org/public/igem/BBa_K538001/1

BBa_R0010
type: Component
persistent identity: component2219865
display id: component2219865
version: 1
top level: LacI:Cpn60
access: public
definition: https://synbiohub.org/public/igem/BBa_R0010/1

BBa_K538001
type: SequenceAnnotation
persistent identity: annotation2219877
display id: annotation2219877
version: 1
top level: LacI:Cpn60
location: https://synbiohub.org/public/igem/BBa_K538201/annotation2219877/range2219877/1
component: BBa_K538001

https://synbiohub.org/public/igem/BBa_K538201/annotation2219877/range2219877/1
type: Range
persistent identity: range2219877
display id: range2219877
version: 1
direction: direction/reverse
top level: LacI:Cpn60
start: 227
end: 1876
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2219873
display id: annotation2219873
version: 1
top level: LacI:Cpn60
location: https://synbiohub.org/public/igem/BBa_K538201/annotation2219873/range2219873/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K538201/annotation2219873/range2219873/1
type: Range
persistent identity: range2219873
display id: range2219873
version: 1
direction: direction/reverse
top level: LacI:Cpn60
start: 209
end: 220
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation2219865
display id: annotation2219865
version: 1
top level: LacI:Cpn60
location: https://synbiohub.org/public/igem/BBa_K538201/annotation2219865/range2219865/1
component: BBa_R0010

https://synbiohub.org/public/igem/BBa_K538201/annotation2219865/range2219865/1
type: Range
persistent identity: range2219865
display id: range2219865
version: 1
direction: direction/forward
top level: LacI:Cpn60
start: 1
end: 200
orientation: inline

LacI:CspA
type: ComponentDefinition
persistent identity: BBa K538203
display id: BBa_K538203
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pLacI + RBS + CspA
creator: Bas Stringer
created: 2011-09-07T11:00:00Z
modified: 2015-05-08T01:12:37Z
discontinued: false
dominant: false
experience: experience/None
group u list: _704_
m user id: 0
owner id: 9985
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: -
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K538003
component: BBa_R0010
component: BBa_B0034
sequence annotation: BBa_K538003
sequence annotation: BBa_B0034
sequence annotation: BBa_R0010
sequence: https://synbiohub.org/public/igem/BBa_K538203_sequence/1

BBa_K538003
type: Component
persistent identity: component2134071
display id: component2134071
version: 1
top level: LacI:CspA
access: public
definition: https://synbiohub.org/public/igem/BBa_K538003/1

BBa_R0010
type: Component
persistent identity: component2134058
display id: component2134058
version: 1
top level: LacI:CspA
access: public
definition: https://synbiohub.org/public/igem/BBa_R0010/1

BBa_B0034
type: Component
persistent identity: component2134066
display id: component2134066
version: 1
top level: LacI:CspA
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K538003
type: SequenceAnnotation
persistent identity: annotation2134071
display id: annotation2134071
version: 1
top level: LacI:CspA
location: https://synbiohub.org/public/igem/BBa_K538203/annotation2134071/range2134071/1
component: BBa_K538003

https://synbiohub.org/public/igem/BBa_K538203/annotation2134071/range2134071/1
type: Range
persistent identity: range2134071
display id: range2134071
version: 1
direction: direction/reverse
top level: LacI:CspA
start: 227
end: 421
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2134066
display id: annotation2134066
version: 1
top level: LacI:CspA
location: https://synbiohub.org/public/igem/BBa_K538203/annotation2134066/range2134066/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K538203/annotation2134066/range2134066/1
type: Range
persistent identity: range2134066
display id: range2134066
version: 1
direction: direction/reverse
top level: LacI:CspA
start: 209
end: 220
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation2134058
display id: annotation2134058
version: 1
top level: LacI:CspA
location: https://synbiohub.org/public/igem/BBa_K538203/annotation2134058/range2134058/1
component: BBa_R0010

https://synbiohub.org/public/igem/BBa_K538203/annotation2134058/range2134058/1
type: Range
persistent identity: range2134058
display id: range2134058
version: 1
direction: direction/forward
top level: LacI:CspA
start: 1
end: 200
orientation: inline

LacI:SDnaK
type: ComponentDefinition
persistent identity: BBa K538202
display id: BBa_K538202
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pLacI + RBS + SheDnaK
creator: Bas Stringer
created: 2011-09-07T11:00:00Z
modified: 2015-05-08T01:12:37Z
discontinued: false
dominant: false
experience: experience/None
group u list: _704_
m user id: 0
owner id: 9985
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: -
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K538002
component: BBa_B0034
component: BBa_R0010
sequence annotation: BBa_K538002
sequence annotation: BBa_B0034
sequence annotation: BBa_R0010
sequence: https://synbiohub.org/public/igem/BBa_K538202_sequence/1

BBa_K538002
type: Component
persistent identity: component2126753
display id: component2126753
version: 1
top level: LacI:SDnaK
access: public
definition: https://synbiohub.org/public/igem/BBa_K538002/1

BBa_B0034
type: Component
persistent identity: component2126750
display id: component2126750
version: 1
top level: LacI:SDnaK
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_R0010
type: Component
persistent identity: component2126742
display id: component2126742
version: 1
top level: LacI:SDnaK
access: public
definition: https://synbiohub.org/public/igem/BBa_R0010/1

BBa_K538002
type: SequenceAnnotation
persistent identity: annotation2126753
display id: annotation2126753
version: 1
top level: LacI:SDnaK
location: https://synbiohub.org/public/igem/BBa_K538202/annotation2126753/range2126753/1
component: BBa_K538002

https://synbiohub.org/public/igem/BBa_K538202/annotation2126753/range2126753/1
type: Range
persistent identity: range2126753
display id: range2126753
version: 1
direction: direction/reverse
top level: LacI:SDnaK
start: 227
end: 2146
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2126750
display id: annotation2126750
version: 1
top level: LacI:SDnaK
location: https://synbiohub.org/public/igem/BBa_K538202/annotation2126750/range2126750/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K538202/annotation2126750/range2126750/1
type: Range
persistent identity: range2126750
display id: range2126750
version: 1
direction: direction/reverse
top level: LacI:SDnaK
start: 209
end: 220
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation2126742
display id: annotation2126742
version: 1
top level: LacI:SDnaK
location: https://synbiohub.org/public/igem/BBa_K538202/annotation2126742/range2126742/1
component: BBa_R0010

https://synbiohub.org/public/igem/BBa_K538202/annotation2126742/range2126742/1
type: Range
persistent identity: range2126742
display id: range2126742
version: 1
direction: direction/forward
top level: LacI:SDnaK
start: 1
end: 200
orientation: inline

BBa_K499596
type: ComponentDefinition
persistent identity: BBa K499596
display id: BBa_K499596
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: luxC (acyl CoA reductase) with a strong promoter
creator: Andrew Perry
created: 2010-06-25T11:00:00Z
modified: 2015-05-08T01:12:30Z
discontinued: false
dominant: false
group u list: _583_
m user id: 0
owner id: 6167
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: As per BBa_K499595, with a T7 promoter.
mutable notes: We want lots of this, so we used a strong promoter.
mutable provenance: gi|59714106
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_I712074
component: BBa_K499595
component: BBa_B0010
component: BBa_B0012
sequence annotation: BBa_K499595
sequence annotation: BBa_I712074
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_K499596_sequence/1

BBa_I712074
type: Component
persistent identity: component2071577
display id: component2071577
version: 1
top level: BBa_K499596
access: public
definition: https://synbiohub.org/public/igem/BBa_I712074/1

BBa_K499595
type: Component
persistent identity: component2071579
display id: component2071579
version: 1
top level: BBa_K499596
access: public
definition: https://synbiohub.org/public/igem/BBa_K499595/1

BBa_B0010
type: Component
persistent identity: component2071580
display id: component2071580
version: 1
top level: BBa_K499596
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0012
type: Component
persistent identity: component2071582
display id: component2071582
version: 1
top level: BBa_K499596
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_K499595
type: SequenceAnnotation
persistent identity: annotation2071579
display id: annotation2071579
version: 1
top level: BBa_K499596
location: https://synbiohub.org/public/igem/BBa_K499596/annotation2071579/range2071579/1
component: BBa_K499595

https://synbiohub.org/public/igem/BBa_K499596/annotation2071579/range2071579/1
type: Range
persistent identity: range2071579
display id: range2071579
version: 1
direction: direction/reverse
top level: BBa_K499596
start: 53
end: 1501
orientation: inline

BBa_I712074
type: SequenceAnnotation
persistent identity: annotation2071577
display id: annotation2071577
version: 1
top level: BBa_K499596
location: https://synbiohub.org/public/igem/BBa_K499596/annotation2071577/range2071577/1
component: BBa_I712074

https://synbiohub.org/public/igem/BBa_K499596/annotation2071577/range2071577/1
type: Range
persistent identity: range2071577
display id: range2071577
version: 1
direction: direction/reverse
top level: BBa_K499596
start: 1
end: 46
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2071580
display id: annotation2071580
version: 1
top level: BBa_K499596
location: https://synbiohub.org/public/igem/BBa_K499596/annotation2071580/range2071580/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K499596/annotation2071580/range2071580/1
type: Range
persistent identity: range2071580
display id: range2071580
version: 1
direction: direction/forward
top level: BBa_K499596
start: 1510
end: 1589
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2071582
display id: annotation2071582
version: 1
top level: BBa_K499596
location: https://synbiohub.org/public/igem/BBa_K499596/annotation2071582/range2071582/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K499596/annotation2071582/range2071582/1
type: Range
persistent identity: range2071582
display id: range2071582
version: 1
direction: direction/forward
top level: BBa_K499596
start: 1598
end: 1638
orientation: inline

BBa_K732002
type: ComponentDefinition
persistent identity: BBa K732002
display id: BBa_K732002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: synCG-β + Promoter + RBS
creator: Joshua Fass
created: 2012-09-30T11:00:00Z
modified: 2015-05-08T01:13:06Z
discontinued: false
dominant: false
experience: experience/Fails
group u list: _978_
m user id: 0
owner id: 9227
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This construct contains a strong constitutive promoter (J23119) as well as an RBS (B0034) upstream of synCG-β (K732001), and should strongly express a synthetic version of the beta subunit of human chorionic gonadotropin.
mutable notes: The promoter and RBS will drive and enable efficient expression of the synthetic coding region.
mutable provenance: Three BioBricks, one of which is from T7 bacteriophage, another of which is derived from a human gene.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K732001
component: BBa_J23119
component: BBa_B0034
sequence annotation: BBa_K732001
sequence annotation: BBa_B0034
sequence annotation: BBa_J23119
sequence: https://synbiohub.org/public/igem/BBa_K732002_sequence/1

BBa_K732001
type: Component
persistent identity: component2205268
display id: component2205268
version: 1
top level: BBa_K732002
access: public
definition: https://synbiohub.org/public/igem/BBa_K732001/1

BBa_J23119
type: Component
persistent identity: component2205265
display id: component2205265
version: 1
top level: BBa_K732002
access: public
definition: https://synbiohub.org/public/igem/BBa_J23119/1

BBa_B0034
type: Component
persistent identity: component2205267
display id: component2205267
version: 1
top level: BBa_K732002
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K732001
type: SequenceAnnotation
persistent identity: annotation2205268
display id: annotation2205268
version: 1
top level: BBa_K732002
location: https://synbiohub.org/public/igem/BBa_K732002/annotation2205268/range2205268/1
component: BBa_K732001

https://synbiohub.org/public/igem/BBa_K732002/annotation2205268/range2205268/1
type: Range
persistent identity: range2205268
display id: range2205268
version: 1
direction: direction/reverse
top level: BBa_K732002
start: 62
end: 559
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2205267
display id: annotation2205267
version: 1
top level: BBa_K732002
location: https://synbiohub.org/public/igem/BBa_K732002/annotation2205267/range2205267/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K732002/annotation2205267/range2205267/1
type: Range
persistent identity: range2205267
display id: range2205267
version: 1
direction: direction/reverse
top level: BBa_K732002
start: 44
end: 55
orientation: inline

BBa_J23119
type: SequenceAnnotation
persistent identity: annotation2205265
display id: annotation2205265
version: 1
top level: BBa_K732002
location: https://synbiohub.org/public/igem/BBa_K732002/annotation2205265/range2205265/1
component: BBa_J23119

https://synbiohub.org/public/igem/BBa_K732002/annotation2205265/range2205265/1
type: Range
persistent identity: range2205265
display id: range2205265
version: 1
direction: direction/reverse
top level: BBa_K732002
start: 1
end: 35
orientation: inline

BBa_K283024
type: ComponentDefinition
persistent identity: BBa K283024
display id: BBa_K283024
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pTet-rbs-SsrB
creator: Shi Lei, W Ling , Nelson Chu
created: 2009-10-12T11:00:00Z
modified: 2015-05-08T01:11:47Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _383_
m user id: 0
owner id: 5494
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: ssrB protein under pTet promoter. The ssrB protein can activate the prosseA promoter. The pTet promoter can be repressed by TetR repressor protein. The ssrB coding region does not contain any terminator
mutable notes: BBa_K283024
mutable provenance: The ssrB coding region is self-designed The rbs and the pTet promoter are from the registry
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: ssrB
sequence annotation: -35
sequence annotation: TetR 1
sequence annotation: conserved
sequence annotation: BBa_B0034
sequence annotation: TetR 2
sequence annotation: -10
sequence annotation: BBa_R0040
sequence: https://synbiohub.org/public/igem/BBa_K283024_sequence/1

ssrB
type: SequenceAnnotation
persistent identity: annotation2040403
display id: annotation2040403
version: 1
top level: BBa_K283024
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K283024/annotation2040403/range2040403/1

https://synbiohub.org/public/igem/BBa_K283024/annotation2040403/range2040403/1
type: Range
persistent identity: range2040403
display id: range2040403
version: 1
direction: direction/forward
top level: BBa_K283024
start: 75
end: 702
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2035712
display id: annotation2035712
version: 1
top level: BBa_K283024
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K283024/annotation2035712/range2035712/1

https://synbiohub.org/public/igem/BBa_K283024/annotation2035712/range2035712/1
type: Range
persistent identity: range2035712
display id: range2035712
version: 1
direction: direction/forward
top level: BBa_K283024
start: 20
end: 25
orientation: inline

TetR 1
type: SequenceAnnotation
persistent identity: annotation2035711
display id: annotation2035711
version: 1
top level: BBa_K283024
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K283024/annotation2035711/range2035711/1

https://synbiohub.org/public/igem/BBa_K283024/annotation2035711/range2035711/1
type: Range
persistent identity: range2035711
display id: range2035711
version: 1
direction: direction/forward
top level: BBa_K283024
start: 1
end: 19
orientation: inline

conserved
type: SequenceAnnotation
persistent identity: annotation2035715
display id: annotation2035715
version: 1
top level: BBa_K283024
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K283024/annotation2035715/range2035715/1

https://synbiohub.org/public/igem/BBa_K283024/annotation2035715/range2035715/1
type: Range
persistent identity: range2035715
display id: range2035715
version: 1
direction: direction/forward
top level: BBa_K283024
start: 67
end: 70
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2035716
display id: annotation2035716
version: 1
top level: BBa_K283024
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K283024/annotation2035716/range2035716/1

https://synbiohub.org/public/igem/BBa_K283024/annotation2035716/range2035716/1
type: Range
persistent identity: range2035716
display id: range2035716
version: 1
direction: direction/reverse
top level: BBa_K283024
start: 63
end: 74
orientation: inline

TetR 2
type: SequenceAnnotation
persistent identity: annotation2035713
display id: annotation2035713
version: 1
top level: BBa_K283024
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K283024/annotation2035713/range2035713/1

https://synbiohub.org/public/igem/BBa_K283024/annotation2035713/range2035713/1
type: Range
persistent identity: range2035713
display id: range2035713
version: 1
direction: direction/forward
top level: BBa_K283024
start: 26
end: 44
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2035714
display id: annotation2035714
version: 1
top level: BBa_K283024
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K283024/annotation2035714/range2035714/1

https://synbiohub.org/public/igem/BBa_K283024/annotation2035714/range2035714/1
type: Range
persistent identity: range2035714
display id: range2035714
version: 1
direction: direction/forward
top level: BBa_K283024
start: 43
end: 48
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation2035710
display id: annotation2035710
version: 1
top level: BBa_K283024
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K283024/annotation2035710/range2035710/1

https://synbiohub.org/public/igem/BBa_K283024/annotation2035710/range2035710/1
type: Range
persistent identity: range2035710
display id: range2035710
version: 1
direction: direction/forward
top level: BBa_K283024
start: 1
end: 54
orientation: inline

BBa_K283011
type: ComponentDefinition
persistent identity: BBa K283011
display id: BBa_K283011
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pTet-rbs-lpp_omp(46-159)_streptavidin_Histag
creator: Shi Lei, W Ling , Nelson Chu
created: 2009-10-12T11:00:00Z
modified: 2015-05-08T01:11:47Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _383_
m user id: 0
owner id: 5494
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: The fusion protein omp(46-159)streptavidin(WT) with a 6-histidine tail is linked after a pTet promoter. The fusion protein coding region does not contain any terminator. The omp domain directs the expression of the fusion protein on the surface membrane
mutable notes: BBa_K283011
mutable provenance: The pTet promoter, rbs are from the registry The fusion protein is self-designed
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_J36837
component: BBa_R0040
component: BBa_J36841
component: BBa_B0034
component: BBa_J36835
sequence annotation: BBa_J36835
sequence annotation: BBa_J36837
sequence annotation: BBa_R0040
sequence annotation: BBa_B0034
sequence annotation: BBa_J36841
sequence: https://synbiohub.org/public/igem/BBa_K283011_sequence/1

BBa_J36837
type: Component
persistent identity: component2055666
display id: component2055666
version: 1
top level: BBa_K283011
access: public
definition: https://synbiohub.org/public/igem/BBa_J36837/1

BBa_R0040
type: Component
persistent identity: component2055658
display id: component2055658
version: 1
top level: BBa_K283011
access: public
definition: https://synbiohub.org/public/igem/BBa_R0040/1

BBa_J36841
type: Component
persistent identity: component2055667
display id: component2055667
version: 1
top level: BBa_K283011
access: public
definition: https://synbiohub.org/public/igem/BBa_J36841/1

BBa_B0034
type: Component
persistent identity: component2055664
display id: component2055664
version: 1
top level: BBa_K283011
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_J36835
type: Component
persistent identity: component2055665
display id: component2055665
version: 1
top level: BBa_K283011
access: public
definition: https://synbiohub.org/public/igem/BBa_J36835/1

BBa_J36835
type: SequenceAnnotation
persistent identity: annotation2055665
display id: annotation2055665
version: 1
top level: BBa_K283011
location: https://synbiohub.org/public/igem/BBa_K283011/annotation2055665/range2055665/1
component: BBa_J36835

https://synbiohub.org/public/igem/BBa_K283011/annotation2055665/range2055665/1
type: Range
persistent identity: range2055665
display id: range2055665
version: 1
direction: direction/reverse
top level: BBa_K283011
start: 81
end: 167
orientation: inline

BBa_J36837
type: SequenceAnnotation
persistent identity: annotation2055666
display id: annotation2055666
version: 1
top level: BBa_K283011
location: https://synbiohub.org/public/igem/BBa_K283011/annotation2055666/range2055666/1
component: BBa_J36837

https://synbiohub.org/public/igem/BBa_K283011/annotation2055666/range2055666/1
type: Range
persistent identity: range2055666
display id: range2055666
version: 1
direction: direction/reverse
top level: BBa_K283011
start: 176
end: 517
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation2055658
display id: annotation2055658
version: 1
top level: BBa_K283011
location: https://synbiohub.org/public/igem/BBa_K283011/annotation2055658/range2055658/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_K283011/annotation2055658/range2055658/1
type: Range
persistent identity: range2055658
display id: range2055658
version: 1
direction: direction/forward
top level: BBa_K283011
start: 1
end: 54
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2055664
display id: annotation2055664
version: 1
top level: BBa_K283011
location: https://synbiohub.org/public/igem/BBa_K283011/annotation2055664/range2055664/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K283011/annotation2055664/range2055664/1
type: Range
persistent identity: range2055664
display id: range2055664
version: 1
direction: direction/reverse
top level: BBa_K283011
start: 63
end: 74
orientation: inline

BBa_J36841
type: SequenceAnnotation
persistent identity: annotation2055667
display id: annotation2055667
version: 1
top level: BBa_K283011
location: https://synbiohub.org/public/igem/BBa_K283011/annotation2055667/range2055667/1
component: BBa_J36841

https://synbiohub.org/public/igem/BBa_K283011/annotation2055667/range2055667/1
type: Range
persistent identity: range2055667
display id: range2055667
version: 1
direction: direction/reverse
top level: BBa_K283011
start: 526
end: 930
orientation: inline

ninabGen
type: ComponentDefinition
persistent identity: BBa K343005
display id: BBa_K343005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Beta-carotene 15
creator: Christian Kurtzhals
created: 2010-09-06T11:00:00Z
modified: 2015-05-08T01:12:08Z
discontinued: false
dominant: false
experience: experience/Issues
group u list: _465_
m user id: 0
owner id: 6083
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: The Beta-carotene 15'15'-monooxygenase generator from the D. melanogaster ninaB gene, cleaves beta-carotene into two retinal molecules. Used together with BBa_274210 (beta-carotene generator) it should induce retinal biosynthesis in e. coli.
mutable notes: A constitutive sigma-70 promotor was chosen. Terminator is lacking on this part.
mutable provenance: [link:https://dgrc.cgb.indiana.edu/product/View?product=4630 Drosophila genomics ressource centre], Indiana University. Found via [link:http://www.flybase.org flybase], ID: [link:http://flybase.org/reports/FBcl0129473.html FBcl0129473]
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K343001
component: BBa_R0040
component: BBa_B0034
sequence annotation: BBa_K343001
sequence annotation: BBa_B0034
sequence annotation: BBa_R0040
sequence: https://synbiohub.org/public/igem/BBa_K343005_sequence/1

BBa_K343001
type: Component
persistent identity: component2079710
display id: component2079710
version: 1
top level: ninabGen
access: public
definition: https://synbiohub.org/public/igem/BBa_K343001/1

BBa_R0040
type: Component
persistent identity: component2079700
display id: component2079700
version: 1
top level: ninabGen
access: public
definition: https://synbiohub.org/public/igem/BBa_R0040/1

BBa_B0034
type: Component
persistent identity: component2079706
display id: component2079706
version: 1
top level: ninabGen
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K343001
type: SequenceAnnotation
persistent identity: annotation2079710
display id: annotation2079710
version: 1
top level: ninabGen
location: https://synbiohub.org/public/igem/BBa_K343005/annotation2079710/range2079710/1
component: BBa_K343001

https://synbiohub.org/public/igem/BBa_K343005/annotation2079710/range2079710/1
type: Range
persistent identity: range2079710
display id: range2079710
version: 1
direction: direction/reverse
top level: ninabGen
start: 81
end: 1943
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2079706
display id: annotation2079706
version: 1
top level: ninabGen
location: https://synbiohub.org/public/igem/BBa_K343005/annotation2079706/range2079706/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K343005/annotation2079706/range2079706/1
type: Range
persistent identity: range2079706
display id: range2079706
version: 1
direction: direction/reverse
top level: ninabGen
start: 63
end: 74
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation2079700
display id: annotation2079700
version: 1
top level: ninabGen
location: https://synbiohub.org/public/igem/BBa_K343005/annotation2079700/range2079700/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_K343005/annotation2079700/range2079700/1
type: Range
persistent identity: range2079700
display id: range2079700
version: 1
direction: direction/forward
top level: ninabGen
start: 1
end: 54
orientation: inline

Plac-sam8
type: ComponentDefinition
persistent identity: BBa I742144
display id: BBa_I742144
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Plac+lacZ+sam8 (tyrosine ammonia lyase)
creator: Chris French
created: 2007-10-22T11:00:00Z
modified: 2015-08-31T04:08:03Z
discontinued: false
dominant: false
experience: experience/None
group u list: _123_
m user id: 0
owner id: 837
owning group id: 163
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This part consists of sam8, encoding tyrosine ammonia lyase (converts tyrosine to p-coumaric acid) from the bacterium Saccharothrix espanaensis DSM 44229, inserted downstream of a lac promoter with attached lacZ' minigene. The purpose of this was to ensure sam8 expression on induction with IPTG and also to provide a visually selectable tag when assembling the entire pathway; by always inserting a lacZ'-containing part into a non-lacZ'-containing part it is easy to select the correct clones on the basis that they form blue colonies.
mutable notes: No important considerations.
mutable provenance: sam8 is derived from Saccharothix espanaensis DSM 44229. The lac promoter and lacZ' minigene, encoding the N-terminal 76 amino acid residues of LacZ, are derived from Escherichia coli BS(DE3).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_I742148
component: BBa_J33207
sequence annotation: BBa_J33207
sequence annotation: BBa_I742148
sequence: https://synbiohub.org/public/igem/BBa_I742144_sequence/1

BBa_I742148
type: Component
persistent identity: component2219402
display id: component2219402
version: 1
top level: Plac-sam8
access: public
definition: https://synbiohub.org/public/igem/BBa_I742148/1

BBa_J33207
type: Component
persistent identity: component2219399
display id: component2219399
version: 1
top level: Plac-sam8
access: public
definition: https://synbiohub.org/public/igem/BBa_J33207/1

BBa_J33207
type: SequenceAnnotation
persistent identity: annotation2219399
display id: annotation2219399
version: 1
top level: Plac-sam8
location: https://synbiohub.org/public/igem/BBa_I742144/annotation2219399/range2219399/1
component: BBa_J33207

https://synbiohub.org/public/igem/BBa_I742144/annotation2219399/range2219399/1
type: Range
persistent identity: range2219399
display id: range2219399
version: 1
direction: direction/reverse
top level: Plac-sam8
start: 1
end: 600
orientation: inline

BBa_I742148
type: SequenceAnnotation
persistent identity: annotation2219402
display id: annotation2219402
version: 1
top level: Plac-sam8
location: https://synbiohub.org/public/igem/BBa_I742144/annotation2219402/range2219402/1
component: BBa_I742148

https://synbiohub.org/public/igem/BBa_I742144/annotation2219402/range2219402/1
type: Range
persistent identity: range2219402
display id: range2219402
version: 1
direction: direction/reverse
top level: Plac-sam8
start: 609
end: 2158
orientation: inline

BBa_K283022
type: ComponentDefinition
persistent identity: BBa K283022
display id: BBa_K283022
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pLacI-rbs-cheZ
creator: Nelson Chu, W Ling , Shi Lei
created: 2009-10-12T11:00:00Z
modified: 2015-05-08T01:11:47Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _383_
m user id: 0
owner id: 5494
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This part consist of a chez downstream of a pLac promoter. The cheZ protein is responsible for the dephosphorylation of cheY protein in bacteria flagella movement. The pLac promoter can be repressed by LacI protein
mutable notes: BBa_K283022
mutable provenance: The chez coding region is self-designed The rbs and the pLac promomter are from the registry
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0034
component: BBa_K094100
component: BBa_R0010
sequence annotation: BBa_R0010
sequence annotation: BBa_B0034
sequence annotation: BBa_K094100
sequence: https://synbiohub.org/public/igem/BBa_K283022_sequence/1

BBa_B0034
type: Component
persistent identity: component2035707
display id: component2035707
version: 1
top level: BBa_K283022
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K094100
type: Component
persistent identity: component2035709
display id: component2035709
version: 1
top level: BBa_K283022
access: public
definition: https://synbiohub.org/public/igem/BBa_K094100/1

BBa_R0010
type: Component
persistent identity: component2035699
display id: component2035699
version: 1
top level: BBa_K283022
access: public
definition: https://synbiohub.org/public/igem/BBa_R0010/1

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation2035699
display id: annotation2035699
version: 1
top level: BBa_K283022
location: https://synbiohub.org/public/igem/BBa_K283022/annotation2035699/range2035699/1
component: BBa_R0010

https://synbiohub.org/public/igem/BBa_K283022/annotation2035699/range2035699/1
type: Range
persistent identity: range2035699
display id: range2035699
version: 1
direction: direction/forward
top level: BBa_K283022
start: 1
end: 200
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2035707
display id: annotation2035707
version: 1
top level: BBa_K283022
location: https://synbiohub.org/public/igem/BBa_K283022/annotation2035707/range2035707/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K283022/annotation2035707/range2035707/1
type: Range
persistent identity: range2035707
display id: range2035707
version: 1
direction: direction/reverse
top level: BBa_K283022
start: 209
end: 220
orientation: inline

BBa_K094100
type: SequenceAnnotation
persistent identity: annotation2035709
display id: annotation2035709
version: 1
top level: BBa_K283022
location: https://synbiohub.org/public/igem/BBa_K283022/annotation2035709/range2035709/1
component: BBa_K094100

https://synbiohub.org/public/igem/BBa_K283022/annotation2035709/range2035709/1
type: Range
persistent identity: range2035709
display id: range2035709
version: 1
direction: direction/reverse
top level: BBa_K283022
start: 227
end: 921
orientation: inline

LacI:CspC
type: ComponentDefinition
persistent identity: BBa K538204
display id: BBa_K538204
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pLacI + RBS + CspC
creator: Bas Stringer
created: 2011-09-07T11:00:00Z
modified: 2015-05-08T01:12:37Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _704_
m user id: 0
owner id: 9985
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: -
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K538004
component: BBa_R0010
component: BBa_B0034
sequence annotation: BBa_B0034
sequence annotation: BBa_R0010
sequence annotation: BBa_K538004
sequence: https://synbiohub.org/public/igem/BBa_K538204_sequence/1

BBa_K538004
type: Component
persistent identity: component2134086
display id: component2134086
version: 1
top level: LacI:CspC
access: public
definition: https://synbiohub.org/public/igem/BBa_K538004/1

BBa_R0010
type: Component
persistent identity: component2134072
display id: component2134072
version: 1
top level: LacI:CspC
access: public
definition: https://synbiohub.org/public/igem/BBa_R0010/1

BBa_B0034
type: Component
persistent identity: component2134080
display id: component2134080
version: 1
top level: LacI:CspC
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2134080
display id: annotation2134080
version: 1
top level: LacI:CspC
location: https://synbiohub.org/public/igem/BBa_K538204/annotation2134080/range2134080/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K538204/annotation2134080/range2134080/1
type: Range
persistent identity: range2134080
display id: range2134080
version: 1
direction: direction/reverse
top level: LacI:CspC
start: 209
end: 220
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation2134072
display id: annotation2134072
version: 1
top level: LacI:CspC
location: https://synbiohub.org/public/igem/BBa_K538204/annotation2134072/range2134072/1
component: BBa_R0010

https://synbiohub.org/public/igem/BBa_K538204/annotation2134072/range2134072/1
type: Range
persistent identity: range2134072
display id: range2134072
version: 1
direction: direction/forward
top level: LacI:CspC
start: 1
end: 200
orientation: inline

BBa_K538004
type: SequenceAnnotation
persistent identity: annotation2134086
display id: annotation2134086
version: 1
top level: LacI:CspC
location: https://synbiohub.org/public/igem/BBa_K538204/annotation2134086/range2134086/1
component: BBa_K538004

https://synbiohub.org/public/igem/BBa_K538204/annotation2134086/range2134086/1
type: Range
persistent identity: range2134086
display id: range2134086
version: 1
direction: direction/reverse
top level: LacI:CspC
start: 227
end: 421
orientation: inline

pBAD:Cpn60
type: ComponentDefinition
persistent identity: BBa K538301
display id: BBa_K538301
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pBAD + RBS + Cpn60
creator: Bas Stringer
created: 2011-09-07T11:00:00Z
modified: 2015-05-08T01:12:37Z
discontinued: false
dominant: false
experience: experience/None
group u list: _704_
m user id: 0
owner id: 9985
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: -
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0034
component: BBa_K538001
component: BBa_K206000
sequence annotation: BBa_B0034
sequence annotation: BBa_K206000
sequence annotation: BBa_K538001
sequence: https://synbiohub.org/public/igem/BBa_K538301_sequence/1

BBa_B0034
type: Component
persistent identity: component2219883
display id: component2219883
version: 1
top level: pBAD:Cpn60
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K538001
type: Component
persistent identity: component2219887
display id: component2219887
version: 1
top level: pBAD:Cpn60
access: public
definition: https://synbiohub.org/public/igem/BBa_K538001/1

BBa_K206000
type: Component
persistent identity: component2219881
display id: component2219881
version: 1
top level: pBAD:Cpn60
access: public
definition: https://synbiohub.org/public/igem/BBa_K206000/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2219883
display id: annotation2219883
version: 1
top level: pBAD:Cpn60
location: https://synbiohub.org/public/igem/BBa_K538301/annotation2219883/range2219883/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K538301/annotation2219883/range2219883/1
type: Range
persistent identity: range2219883
display id: range2219883
version: 1
direction: direction/reverse
top level: pBAD:Cpn60
start: 139
end: 150
orientation: inline

BBa_K206000
type: SequenceAnnotation
persistent identity: annotation2219881
display id: annotation2219881
version: 1
top level: pBAD:Cpn60
location: https://synbiohub.org/public/igem/BBa_K538301/annotation2219881/range2219881/1
component: BBa_K206000

https://synbiohub.org/public/igem/BBa_K538301/annotation2219881/range2219881/1
type: Range
persistent identity: range2219881
display id: range2219881
version: 1
direction: direction/reverse
top level: pBAD:Cpn60
start: 1
end: 130
orientation: inline

BBa_K538001
type: SequenceAnnotation
persistent identity: annotation2219887
display id: annotation2219887
version: 1
top level: pBAD:Cpn60
location: https://synbiohub.org/public/igem/BBa_K538301/annotation2219887/range2219887/1
component: BBa_K538001

https://synbiohub.org/public/igem/BBa_K538301/annotation2219887/range2219887/1
type: Range
persistent identity: range2219887
display id: range2219887
version: 1
direction: direction/reverse
top level: pBAD:Cpn60
start: 157
end: 1806
orientation: inline

LacI:Cpn10
type: ComponentDefinition
persistent identity: BBa K538200
display id: BBa_K538200
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pLacI + RBS + Cpn10
creator: Bas Stringer
created: 2011-09-07T11:00:00Z
modified: 2015-05-08T01:12:37Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _704_
m user id: 0
owner id: 9985
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: -
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_R0010
component: BBa_K538000
component: BBa_B0034
sequence annotation: BBa_R0010
sequence annotation: BBa_B0034
sequence annotation: BBa_K538000
sequence: https://synbiohub.org/public/igem/BBa_K538200_sequence/1

BBa_R0010
type: Component
persistent identity: component2126718
display id: component2126718
version: 1
top level: LacI:Cpn10
access: public
definition: https://synbiohub.org/public/igem/BBa_R0010/1

BBa_K538000
type: Component
persistent identity: component2126729
display id: component2126729
version: 1
top level: LacI:Cpn10
access: public
definition: https://synbiohub.org/public/igem/BBa_K538000/1

BBa_B0034
type: Component
persistent identity: component2126726
display id: component2126726
version: 1
top level: LacI:Cpn10
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation2126718
display id: annotation2126718
version: 1
top level: LacI:Cpn10
location: https://synbiohub.org/public/igem/BBa_K538200/annotation2126718/range2126718/1
component: BBa_R0010

https://synbiohub.org/public/igem/BBa_K538200/annotation2126718/range2126718/1
type: Range
persistent identity: range2126718
display id: range2126718
version: 1
direction: direction/forward
top level: LacI:Cpn10
start: 1
end: 200
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2126726
display id: annotation2126726
version: 1
top level: LacI:Cpn10
location: https://synbiohub.org/public/igem/BBa_K538200/annotation2126726/range2126726/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K538200/annotation2126726/range2126726/1
type: Range
persistent identity: range2126726
display id: range2126726
version: 1
direction: direction/reverse
top level: LacI:Cpn10
start: 209
end: 220
orientation: inline

BBa_K538000
type: SequenceAnnotation
persistent identity: annotation2126729
display id: annotation2126729
version: 1
top level: LacI:Cpn10
location: https://synbiohub.org/public/igem/BBa_K538200/annotation2126729/range2126729/1
component: BBa_K538000

https://synbiohub.org/public/igem/BBa_K538200/annotation2126729/range2126729/1
type: Range
persistent identity: range2126729
display id: range2126729
version: 1
direction: direction/reverse
top level: LacI:Cpn10
start: 227
end: 523
orientation: inline

pBAD:Cpn10
type: ComponentDefinition
persistent identity: BBa K538300
display id: BBa_K538300
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pBAD + RBS + Cpn10
creator: Bas Stringer
created: 2011-09-07T11:00:00Z
modified: 2015-05-08T01:12:37Z
discontinued: false
dominant: false
experience: experience/None
group u list: _704_
m user id: 0
owner id: 9985
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: -
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K538000
component: BBa_K206000
component: BBa_B0034
sequence annotation: BBa_K538000
sequence annotation: BBa_K206000
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K538300_sequence/1

BBa_K538000
type: Component
persistent identity: component2126764
display id: component2126764
version: 1
top level: pBAD:Cpn10
access: public
definition: https://synbiohub.org/public/igem/BBa_K538000/1

BBa_K206000
type: Component
persistent identity: component2126759
display id: component2126759
version: 1
top level: pBAD:Cpn10
access: public
definition: https://synbiohub.org/public/igem/BBa_K206000/1

BBa_B0034
type: Component
persistent identity: component2126761
display id: component2126761
version: 1
top level: pBAD:Cpn10
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K538000
type: SequenceAnnotation
persistent identity: annotation2126764
display id: annotation2126764
version: 1
top level: pBAD:Cpn10
location: https://synbiohub.org/public/igem/BBa_K538300/annotation2126764/range2126764/1
component: BBa_K538000

https://synbiohub.org/public/igem/BBa_K538300/annotation2126764/range2126764/1
type: Range
persistent identity: range2126764
display id: range2126764
version: 1
direction: direction/reverse
top level: pBAD:Cpn10
start: 157
end: 453
orientation: inline

BBa_K206000
type: SequenceAnnotation
persistent identity: annotation2126759
display id: annotation2126759
version: 1
top level: pBAD:Cpn10
location: https://synbiohub.org/public/igem/BBa_K538300/annotation2126759/range2126759/1
component: BBa_K206000

https://synbiohub.org/public/igem/BBa_K538300/annotation2126759/range2126759/1
type: Range
persistent identity: range2126759
display id: range2126759
version: 1
direction: direction/reverse
top level: pBAD:Cpn10
start: 1
end: 130
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2126761
display id: annotation2126761
version: 1
top level: pBAD:Cpn10
location: https://synbiohub.org/public/igem/BBa_K538300/annotation2126761/range2126761/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K538300/annotation2126761/range2126761/1
type: Range
persistent identity: range2126761
display id: range2126761
version: 1
direction: direction/reverse
top level: pBAD:Cpn10
start: 139
end: 150
orientation: inline

BBa_K283005
type: ComponentDefinition
persistent identity: BBa K283005
display id: BBa_K283005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: lpp_ompA_eGFP_streptavidin
creator: Yu bin, Jiang QinQin
created: 2009-10-12T11:00:00Z
modified: 2015-05-08T01:11:47Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _383_
m user id: 0
owner id: 5494
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: This part consists of a fusion protein Lpp-rbs-protein under the pLac promoter. The Lpp is a signal peptide. The ompA domain of the fusion directs the protein to the surface membrane. The pLac promoter can be repressed by LacI protein. This part lacks a terminator.
mutable notes: BBa_K283005
mutable provenance: The pLac promoter, rbs sequence are obtained from the registry The fusion protein coding sequence is self-designed
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_J36837
component: BBa_J36835
component: BBa_K283009
sequence annotation: BBa_J36837
sequence annotation: BBa_J36835
sequence annotation: BBa_K283009
sequence: https://synbiohub.org/public/igem/BBa_K283005_sequence/1

BBa_J36837
type: Component
persistent identity: component2055625
display id: component2055625
version: 1
top level: BBa_K283005
access: public
definition: https://synbiohub.org/public/igem/BBa_J36837/1

BBa_J36835
type: Component
persistent identity: component2055624
display id: component2055624
version: 1
top level: BBa_K283005
access: public
definition: https://synbiohub.org/public/igem/BBa_J36835/1

BBa_K283009
type: Component
persistent identity: component2055627
display id: component2055627
version: 1
top level: BBa_K283005
access: public
definition: https://synbiohub.org/public/igem/BBa_K283009/1

BBa_J36837
type: SequenceAnnotation
persistent identity: annotation2055625
display id: annotation2055625
version: 1
top level: BBa_K283005
location: https://synbiohub.org/public/igem/BBa_K283005/annotation2055625/range2055625/1
component: BBa_J36837

https://synbiohub.org/public/igem/BBa_K283005/annotation2055625/range2055625/1
type: Range
persistent identity: range2055625
display id: range2055625
version: 1
direction: direction/reverse
top level: BBa_K283005
start: 88
end: 429
orientation: inline

BBa_J36835
type: SequenceAnnotation
persistent identity: annotation2055624
display id: annotation2055624
version: 1
top level: BBa_K283005
location: https://synbiohub.org/public/igem/BBa_K283005/annotation2055624/range2055624/1
component: BBa_J36835

https://synbiohub.org/public/igem/BBa_K283005/annotation2055624/range2055624/1
type: Range
persistent identity: range2055624
display id: range2055624
version: 1
direction: direction/reverse
top level: BBa_K283005
start: 1
end: 87
orientation: inline

BBa_K283009
type: SequenceAnnotation
persistent identity: annotation2055627
display id: annotation2055627
version: 1
top level: BBa_K283005
location: https://synbiohub.org/public/igem/BBa_K283005/annotation2055627/range2055627/1
component: BBa_K283009

https://synbiohub.org/public/igem/BBa_K283005/annotation2055627/range2055627/1
type: Range
persistent identity: range2055627
display id: range2055627
version: 1
direction: direction/reverse
top level: BBa_K283005
start: 430
end: 1533
orientation: inline

pBAD:CspA
type: ComponentDefinition
persistent identity: BBa K538303
display id: BBa_K538303
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pBAD + RBS + CspA
creator: Bas Stringer
created: 2011-09-07T11:00:00Z
modified: 2015-05-08T01:12:37Z
discontinued: false
dominant: false
experience: experience/None
group u list: _704_
m user id: 0
owner id: 9985
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: -
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K206000
component: BBa_K538003
component: BBa_B0034
sequence annotation: BBa_B0034
sequence annotation: BBa_K538003
sequence annotation: BBa_K206000
sequence: https://synbiohub.org/public/igem/BBa_K538303_sequence/1

BBa_K206000
type: Component
persistent identity: component2134090
display id: component2134090
version: 1
top level: pBAD:CspA
access: public
definition: https://synbiohub.org/public/igem/BBa_K206000/1

BBa_K538003
type: Component
persistent identity: component2134097
display id: component2134097
version: 1
top level: pBAD:CspA
access: public
definition: https://synbiohub.org/public/igem/BBa_K538003/1

BBa_B0034
type: Component
persistent identity: component2134092
display id: component2134092
version: 1
top level: pBAD:CspA
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2134092
display id: annotation2134092
version: 1
top level: pBAD:CspA
location: https://synbiohub.org/public/igem/BBa_K538303/annotation2134092/range2134092/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K538303/annotation2134092/range2134092/1
type: Range
persistent identity: range2134092
display id: range2134092
version: 1
direction: direction/reverse
top level: pBAD:CspA
start: 139
end: 150
orientation: inline

BBa_K538003
type: SequenceAnnotation
persistent identity: annotation2134097
display id: annotation2134097
version: 1
top level: pBAD:CspA
location: https://synbiohub.org/public/igem/BBa_K538303/annotation2134097/range2134097/1
component: BBa_K538003

https://synbiohub.org/public/igem/BBa_K538303/annotation2134097/range2134097/1
type: Range
persistent identity: range2134097
display id: range2134097
version: 1
direction: direction/reverse
top level: pBAD:CspA
start: 157
end: 351
orientation: inline

BBa_K206000
type: SequenceAnnotation
persistent identity: annotation2134090
display id: annotation2134090
version: 1
top level: pBAD:CspA
location: https://synbiohub.org/public/igem/BBa_K538303/annotation2134090/range2134090/1
component: BBa_K206000

https://synbiohub.org/public/igem/BBa_K538303/annotation2134090/range2134090/1
type: Range
persistent identity: range2134090
display id: range2134090
version: 1
direction: direction/reverse
top level: pBAD:CspA
start: 1
end: 130
orientation: inline