This part consist of a chez downstream of a pLac promoter. The cheZ protein is responsible for the dephosphorylation of cheY protein in bacteria flagella movement. The pLac promoter can be repressed by LacI protein
The chez coding region is self-designed The rbs and the pLac promomter are from the registry
Plac+rbs+glgC (ADP-glucose pyrophosphorylase)
This is the coding sequence of glgC from ''Escherichia coli'' JM109 (see [http://partsregistry.org/wiki/index.php?title=Part:BBa_K118015 BBa_K118015]) with a ribosome binding site coupled to an upstream Lac promoter and ''lacZ''' as a method for testing expression.
THe pTet promoter can be repressed by TetR repressor protein. The cI protein generated can repress plambda(R-O12)(K094002) promoter. This part lacks a terminator
The pTet promoter and the rbs comes from the registry, The cI protein comes from lambda phage
This part consists of sam8, encoding tyrosine ammonia lyase (converts tyrosine to p-coumaric acid) from the bacterium Saccharothrix espanaensis DSM 44229, inserted downstream of a lac promoter with attached lacZ' minigene. The purpose of this was to ensure sam8 expression on induction with IPTG and also to provide a visually selectable tag when assembling the entire pathway; by always inserting a lacZ'-containing part into a non-lacZ'-containing part it is easy to select the correct clones on the basis that they form blue colonies.
sam8 is derived from Saccharothix espanaensis DSM 44229. The lac promoter and lacZ' minigene, encoding the N-terminal 76 amino acid residues of LacZ, are derived from Escherichia coli BS(DE3).
ssrB protein under pTet promoter. The ssrB protein can activate the prosseA promoter. The pTet promoter can be repressed by TetR repressor protein. The ssrB coding region does not contain any terminator
The ssrB coding region is self-designed The rbs and the pTet promoter are from the registry
This construct contains a strong constitutive promoter (J23119) as well as an RBS (B0034) upstream of synCG-β (K732001), and should strongly express a synthetic version of the beta subunit of human chorionic gonadotropin.
The promoter and RBS will drive and enable efficient expression of the synthetic coding region.
Three BioBricks, one of which is from T7 bacteriophage, another of which is derived from a human gene.
Plac+rbs+glgC16 (glgC with G336D substitution))
This is the coding sequence of ''glgC''16 from ''Escherichia coli'' JM109 (see [http://partsregistry.org/wiki/index.php?title=Part:BBa_K118016 BBa_K118016]) with a ribosome binding site coupled to an upstream Lac promoter and ''lacZ''' as a method for testing expression.
This part consists of a fusion protein Lpp-rbs-protein under the pLac promoter. The Lpp is a signal peptide. The ompA domain of the fusion directs the protein to the surface membrane. The pLac promoter can be repressed by LacI protein. This part lacks a terminator.
The pLac promoter, rbs sequence are obtained from the registry The fusion protein coding sequence is self-designed
This part codes for part of the protein invisine. When fused with other protein, it can direct the protein to be expressed on the membrane
The fusion protein omp(46-159)streptavidin(WT) with a 6-histidine tail is linked after a pTet promoter. The fusion protein coding region does not contain any terminator. The omp domain directs the expression of the fusion protein on the surface membrane
The pTet promoter, rbs are from the registry The fusion protein is self-designed
rbs-GFP(LVA)-terminators (Backbone: pSB1AK3)
The chez is responsible for the dephosphorylation of cheY protein in bacteria flagella movement and is tagged by a 6-histidine tail. The pLac promoter can be repressed by LacI repressor protein
The chez-histag coding region is self-designed The pLac promoter , rbs are from the registry
The Beta-carotene 15'15'-monooxygenase generator from the D. melanogaster ninaB gene, cleaves beta-carotene into two retinal molecules. Used together with BBa_274210 (beta-carotene generator) it should induce retinal biosynthesis in e. coli.
A constitutive sigma-70 promotor was chosen. Terminator is lacking on this part.
[link:https://dgrc.cgb.indiana.edu/product/View?product=4630 Drosophila genomics ressource centre], Indiana University. Found via [link:http://www.flybase.org flybase], ID: [link:http://flybase.org/reports/FBcl0129473.html FBcl0129473]
luxC (acyl CoA reductase) with a strong promoter
We want lots of this, so we used a strong promoter.