Stationary-phase-dependent banana odor generator
This part is an ATF1-1148 protein generator under control by the osmY stationary phase promoter (BBa_J45993). It is almost identical to J45250, except that: 1. The promoter is the truncated version of osmY and 2. it uses the B0030 RBS instead of B0032 (which is in J45250).
Olmecs: Inducible expression of HK022 cI repressor
Protein generator converting PoPS to the prot BSMT. The BSMT enzyme converts benzoic acid/salicylic acid to methyl benzoate and methyl salicylate.
This part is phBSMT1 from Petunia x hybrida (GenBank). See part design and discussion for more information.
Lactose/IPTG inducible promoter with GFP reporter tagged with AAV-ssrA degradation tag followed by terminator. The tagged GFP is actively degraded within cell, thus provides better temporal resolution of green fluorescence and promoter activity.
Part was assembled using BrickClip assembly (BBF RFC104) using other biobrick parts as templates. BrickClip assembly is a special case of more general Paperclip assembly method.[1]
Lactose/IPTG inducible promoter with RBS, RFP reporter tagged with LAA-ssrA degradation tag and terminator. The tagged RFP is actively degraded within cell, thus provides better temporal resolution of red fluorescence and promoter activity.
Part was assembled using BrickClip assembly (BBF RFC 104) using other biobrick parts as templates. BrickClip assembly is a special case of more general Paperclip assembly method.[1]
protein generator for biosynthesis of vanillin
Protein generator version of BBa-I744002. strong ribosomal binding site, promoter, and strong terminator
strong ribosome binding site..constitutive promoter...
generates the feruloyl-CoA hydratase for vanillin
Protein generator version of BBa_1738002 Promoter, strong ribosome binding site, and strong terminator.
Strong ribosome binding site, well documented promoter, strong terminator
Lactose/IPTG inducible promoter with RBS, RFP reporter tagged with LVA-ssrA degradation tag and terminator. The tagged RFP is actively degraded within cell, thus provides better temporal resolution of red fluorescence and promoter activity. This part can be considered as improved version of BBa_J04451 as it does not contain StuI restriction site coding for additional 'RP' residues between RFP and LVA tag.
constitutive promoter-rbs-CI (lambda LVA)
This part generates chez protein under a hypoxia inducible promoter panas. The cheZ protein is responsible for the dephosphorylation of cheY protein in bacteria flagella movement and is tagged by a 6-Histidine tail.
The rbs, double terminator comes from the registry
Olmecs: Inducible expression of P22 c2 repressor
pTet-rbs-lpp_ompA_eGFP_streptavidin-double Terminators
This part contains a red fluorescence protein followed by double terminator, the RFP has no preceding rbs sequence
Both the RFP and the terminator are from the registry
generates the tyrosinase for melanin biosynthesis
Registry promoter, RBS, and terminator. this is the generator for tyrosinase gene
ech protein generator for vanillin biosynthesis
This is a part, that helps to synthesize vanillin from ferulic acid with the help of fcs
Needs to be used in conjuction with fcs in order to generate vanillin
uses <partinfo>BBa_I760000</partinfo>, promotor, RBS and terminator. Promotor is EPTG inducible. Contains high efficiency RBS and terminator.
protein generator (EXAMPLE ONLY), version of fimA promoter, strong ribosome binding site, and strong terminator
BBa_J23100 Promoter BBa_B0030 RBS BBa_I731002 FimA BBa_B0014 terminator
Protein generator converting PoPS to the protein ATF1. ATFI encodes the enzyme alcohol acetyltransferase I (AATase I), which produces acetate esters from acetyl CoA and alcohols.
This part is the ATF sequence BBa_45J006 with a single point mutation at base pair 1148 (T -> C). Despite the mutation, the enzyme is still functional (kinetic studies have not been done to determine realitive functionality).
This part was cloned from S. cerevisiae.
Lactose/IPTG inducible promoter wuth RFP reporter tagged with AAV-ssrA degradation tag followed by terminator. The tagged RFP is actively degraded within cell, thus provides better temporal resolution of red fluorescence and promoter activity. Part was assembled using BrickClip assembly (BBF RFC 104) using other biobrick parts as templates. BrickClip assembly is a special case of more general Paperclip assembly method.[1] Reference [1] Trubitsyna, M., Michlewski, G., Cai, Y., Elfick, A. & French, C. E. PaperClip: rapid multi-part DNA assembly from existing libraries. Nucleic Acids Res. 1???8 (2014). doi:10.1093/nar/gku829
Lactose/IPTG inducible promoter with RFP reporter tagged with DAS-ssrA degradation tag followed by terminator. The tagged RFP is degraded within cell, thus provides better temporal resolution of red fluorescence and promoter activity. Presence of SspB is crucial for effective protein degradation.
Lactose/IPTG inducible promoter with GFP reporter tagged with NYADAS-ssrA degradation tag followed by terminator. The tagged GFP is actively degraded within cell, thus provides better temporal resolution of green fluorescence and promoter activity.
uses <partinfo>BBa_I769015</partinfo> as well as a promoter, RBS, and terminator
This coding region comes from Erwinia uredovora.
Tetracycline/aTc inducible promoter with GFP reporter tagged with AAV-ssrA degradation tag followed by terminator. The tagged GFP is actively degraded within cell, thus provides better temporal resolution of green fluorescence and promoter activity.
Butanol dehydrogenase generator, beta-butryaldehyde -> butanol
This part constitutively translates the aad gene, which produces and alcohol dehydrogenase that may be important in the synthesis of butanol from beta-butryaldehyde.
Tetracycline/aTc inducible promoter with GFP reporter tagged with LAA-ssrA degradation tag followed by terminator. The tagged GFP is actively degraded within cell, thus provides better temporal resolution of green fluorescence and promoter activity.
Protein generator converting PoPS to the protein ATF1. ATFI encodes the enzyme alcohol acetyltransferase I (AATase I), which produces acetate esters from acetyl CoA and alcohols. This part is similar to BBa_J45200, except that it uses the B0032 RBS instead of B0030 (which is in BBa_J45200).
Olmecs: Inducible expression of Lambda cI repressor
Olmecs: Inducible expression of 434 cI repressor
This part helps to synthesize vanillin from ferulic acid with the help of fcs.
Needs to be used in conjunction with fcs in order to generate vanillin.
Uses <partinfo>BBa_I769105</partinfo> as well as a promoter, RBS, and terminator. Uses IPTG inducible promoter in order to test construct. Contains high efficiency RBS and terminator.
Tetracycline/aTc inducible promoter with GFP reporter tagged with NYADAS-ssrA degradation tag followed by terminator. The tagged GFP is actively degraded within cell, thus provides better temporal resolution of green fluorescence and promoter activity.
Wintergreen odor enzyme (BSMT1) generator
This is the wintergreen generating device hooked up to a constitutive promoter.
This is made from a biobrick promoter and our biobrick wintergreen generating device.
The pLac promoter can be repressed by LacI protein. The cheZ protein is responsible for the dephosphorylation of cheY protein in bacteria flagella movement and is tagged by 6-Histidine tail.
The chez-Histag coding region is self-designed The pLac promoter, rbs and the double terminator are from the registry
needs to be used in conjuction with fcs in order to generate vanillin.
uses "partinfo" BBa_l75400 as well as a promoter, RBS, and terminator. IPTG induceable promoter inordder to test the conctruct. Strong RBS and terminator.
This part constitutively translates the cel5F gene from Saccharophagus degradans 2-40. The cellulase produced help degrade cellulose into glucose.
This is the wintergreen generating device hooked up to a constitutive promoter with a different RBS (B0032, different from 45100).
Stationary-phase-dependent wintergreen odor generator
This is the wintergreen generating device hooked up to a stationary phase promoter with RBS B0032.
Biobricks of osmY promoter and intermediate 45119.
Still in development as SPP is not confirmed to work.
This part is an ATF1-1148 protein generator under control by the osmY stationary phase promoter. It is almost identical to J45270, except that: 1) The promoter is the truncated version of osmY and 2) it uses the B0030 RBS instead of B0032 (which is in J45270).
This part is triggered by BBa_1711006 (which detects red light) and produces tyrosinase and ORF438, two proteins known to be important for melanogenesis.
The promoter region may have to be modified to be activated by BBa_I711006.
Tetracycline/aTc inducible promoter with GFP reporter tagged with LVA-ssrA degradation tag followed by terminator. The tagged GFP is actively degraded within cell, thus provides better temporal resolution of green fluorescence and promoter activity.
This part consists of a fusion protein invisine-RFP with a double terminator. There is no preceding rbs on the protein coding region.
The invisine coding region is self-designed and the terminators are from the registry
Acetyl-CoA -> acetoacetyl-CoA (thiolase)
This part constitutively produces thiolase, and enzyme responsible for creating acetoacetyl-CoA from acetyl-CoA.
Lactose/IPTG inducible promoter with GFP reporter tagged with DAS-ssrA degradation tag followed by terminator. The tagged GFP is actively degraded within cell, thus provides better temporal resolution of green fluorescence and promoter activity. Presence of SspB is crucial for effective protein degradation.
The chez is constitutively expressed under the pTet promoter. The pTet promoter can be repressed by TetR repressor protein. The cheZ protein is responsible for the dephosphorylation of cheY protein in bacteria flagella movement.
The chez coding region is self-designed The pTet promoter, rbs and the terminator are from the registry
ech My new test generator part Feruloyl CoA hydratase for vanilin
This is the part I am editing in the teaching session. It helps synthesize vanilin from ferulic acid with the help of fcs.
Needs to be used in conjunction with fcs in order to generate vanilin. It uses an ITPG promoter and an high efficiency RBS terminator.
Uses <partinfo>BBa_I71800</partinfo> as well as a promoter, RBS, and terminator.
Expresses B-carotene monooxygenase on a constitutive promotor
This generator expresses a beta-carotene 15,15'-monooxygenase,on a constitutive promotor, that catalyses retinal production from beta-carotene via the following reaction: beta-carotene + O2 = 2 retinal
The brick requires B-carotene supply to function. This can either be supplied externally, or alternatively the BBa_K274210 B-carotene generator will induce full B-carotene synthesis in E. coli.
The ninaB gene was originally cloned from drosophila melanogaster, and cDNA was aquired for production of the biobrick from the Drosophila Genome Ressource Center. Promotor+rbs brick BBa_J13002 and terminator BBa_B0015 were used.
Tetracycline/aTc inducible promoter with GFP reporter tagged with DAS-ssrA degradation tag followed by terminator. The tagged GFP is actively degraded within cell, thus provides better temporal resolution of green fluorescence and promoter activity.Presence of SspB is crucial for effective protein degradation.