//classic/inverter
type: Collection
persistent identity: classic inverter
display id: classic_inverter
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
member: //classic/inverter/uncategorized

//classic/inverter/uncategorized
type: Collection
persistent identity: classic inverter uncategorized
display id: classic_inverter_uncategorized
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
member: BBa_I9004
member: BBa_Q00400
member: BBa_Q04510
member: BBa_Q02121
member: BBa_J06802
member: BBa_Q04122
member: BBa_K1366002
member: BBa_I13409
member: BBa_J06803
member: BBa_Q09400
member: lacI
member: BBa_I9003
member: BBa_Q01121
member: BBa_Q04530
member: BBa_Q01122
member: tetR inver
member: BBa_Q08400
member: BBa_A340620
member: BBa_Q02400
member: BBa_Q04740
member: BBa_J06800
member: BBa_I9002
member: tetR
member: BBa_Q07400
member: BBa_I9001
member: BBa_J06801
member: BBa_Q04520
member: BBa_Q00121
member: cI

lux pR
type: ComponentDefinition
persistent identity: BBa R0062
display id: BBa_R0062
version: 1
was generated by: iGEM to SBOL conversion
description: Promoter (luxR & HSL regulated -- lux pR)
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:15Z
mutable provenance: V. fischeri
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Promoter activated by LuxR in concert with HSL The lux cassette of V. fischeri contains a left and a right promoter. The right promoter gives weak constitutive expression of downstream genes.This expression is up-regulated by the action of the LuxR activator protein complexed with the autoinducer, 3-oxo-hexanoyl-HSL. Two molecules of LuxR protein form a complex with two molecules of the signalling compound homoserine lactone (HSL). This complex binds to a palindromic site on the promoter, increasing the rate of transcription.
discontinued: false
dominant: true
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: This promoter is based on the Vibrio fischeri quorum sensing gene promoters. Two genes LuxI and LuxR and transcribed in opposite directions as shown below. The original sequence from which the parts <bb_part>BBa_R0062</bb_part> and <bb_part>BBa_R0063</bb_part> were derived is shown in the picture below. <img src="<bb_file>Image1.gif</bb_file>" width="614" height="362">
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -10
sequence annotation: start
sequence annotation: -35
sequence annotation: BBa_R0062
sequence annotation: LuxR/HSL
sequence: https://synbiohub.org/public/igem/BBa_R0062_sequence/1

-10
type: SequenceAnnotation
persistent identity: annotation2047
display id: annotation2047
version: 1
top level: lux pR
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0062/annotation2047/range2047/1

https://synbiohub.org/public/igem/BBa_R0062/annotation2047/range2047/1
type: Range
persistent identity: range2047
display id: range2047
version: 1
top level: lux pR
direction: direction/forward
start: 42
end: 47
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2048
display id: annotation2048
version: 1
top level: lux pR
role: feature/start
role: SO:0000318
location: https://synbiohub.org/public/igem/BBa_R0062/annotation2048/range2048/1

https://synbiohub.org/public/igem/BBa_R0062/annotation2048/range2048/1
type: Range
persistent identity: range2048
display id: range2048
version: 1
top level: lux pR
direction: direction/forward
start: 53
end: 53
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2046
display id: annotation2046
version: 1
top level: lux pR
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0062/annotation2046/range2046/1

https://synbiohub.org/public/igem/BBa_R0062/annotation2046/range2046/1
type: Range
persistent identity: range2046
display id: range2046
version: 1
top level: lux pR
direction: direction/forward
start: 20
end: 25
orientation: inline

BBa_R0062
type: SequenceAnnotation
persistent identity: annotation7070
display id: annotation7070
version: 1
top level: lux pR
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_R0062/annotation7070/range7070/1

https://synbiohub.org/public/igem/BBa_R0062/annotation7070/range7070/1
type: Range
persistent identity: range7070
display id: range7070
version: 1
top level: lux pR
direction: direction/forward
start: 1
end: 55
orientation: inline

LuxR/HSL
type: SequenceAnnotation
persistent identity: annotation2045
display id: annotation2045
version: 1
top level: lux pR
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0062/annotation2045/range2045/1

https://synbiohub.org/public/igem/BBa_R0062/annotation2045/range2045/1
type: Range
persistent identity: range2045
display id: range2045
version: 1
top level: lux pR
direction: direction/forward
start: 1
end: 20
orientation: inline

lacI+pL
type: ComponentDefinition
persistent identity: BBa R0011
display id: BBa_R0011
version: 1
was generated by: iGEM to SBOL conversion
description: Promoter (lacI regulated, lambda pL hybrid)
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: represillator of Elowitz and Leibler (2000)
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) The PLlac 0-1 promoter is a hybrid regulatory region consisting of the promoter P(L) of phage lambda with the cI binding sites replaced with lacO1. The hybrid design allows for strong promotion that can nevertheless be tightly repressed by LacI, the Lac inhibitor (i.e. repressor) (<bb_part>BBa_C0010</bb_part>) ([LUTZ97]). The activity of the promoter can be regulated over a >600-fold range by IPTG in E.Coli DH5-alpha-Z1 (same paper reference).
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: hybrid promoter design to create strong promoter that is, at the same time, highly repressible. note that the upstream operator installed in this hybrid is slightly different than the one in the original source (Lutz and Bujard, 1997). the most upstream operator region is slightly truncated in the represillator version, so that both operators in the hybrid are the same sequence. see references for details. also, the sequence has been truncated after the transcriptional start site. LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and increase transcription. This part is incompatible with environments containing lactose or lactose analogs.
star: true
creator: Neelaksh Varshney, Grace Kenney, Daniel Shen, Samantha Sutton
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: lac O1
sequence annotation: -35
sequence annotation: BBa_R0011
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R0011_sequence/1

lac O1
type: SequenceAnnotation
persistent identity: annotation1999
display id: annotation1999
version: 1
top level: lacI+pL
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_R0011/annotation1999/range1999/1

https://synbiohub.org/public/igem/BBa_R0011/annotation1999/range1999/1
type: Range
persistent identity: range1999
display id: range1999
version: 1
top level: lacI+pL
direction: direction/forward
start: 3
end: 19
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2000
display id: annotation2000
version: 1
top level: lacI+pL
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0011/annotation2000/range2000/1

https://synbiohub.org/public/igem/BBa_R0011/annotation2000/range2000/1
type: Range
persistent identity: range2000
display id: range2000
version: 1
top level: lacI+pL
direction: direction/forward
start: 20
end: 25
orientation: inline

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation7064
display id: annotation7064
version: 1
top level: lacI+pL
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_R0011/annotation7064/range7064/1

https://synbiohub.org/public/igem/BBa_R0011/annotation7064/range7064/1
type: Range
persistent identity: range7064
display id: range7064
version: 1
top level: lacI+pL
direction: direction/forward
start: 1
end: 54
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2002
display id: annotation2002
version: 1
top level: lacI+pL
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0011/annotation2002/range2002/1

https://synbiohub.org/public/igem/BBa_R0011/annotation2002/range2002/1
type: Range
persistent identity: range2002
display id: range2002
version: 1
top level: lacI+pL
direction: direction/forward
start: 43
end: 48
orientation: inline

lac O1
type: SequenceAnnotation
persistent identity: annotation2001
display id: annotation2001
version: 1
top level: lacI+pL
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_R0011/annotation2001/range2001/1

https://synbiohub.org/public/igem/BBa_R0011/annotation2001/range2001/1
type: Range
persistent identity: range2001
display id: range2001
version: 1
top level: lacI+pL
direction: direction/forward
start: 26
end: 42
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: T7 TE
sequence annotation: BBa_B0012
sequence annotation: stop
sequence annotation: polya
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

cII p22
type: ComponentDefinition
persistent identity: BBa R0053
display id: BBa_R0053
version: 1
was generated by: iGEM to SBOL conversion
description: Promoter (p22 cII regulated)
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: Bacteriophage p22.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: The p22 cII regulatory region sequence is a 97 base-pair sequence with the standard BioBrick prefix and suffix sections on its ends. p22 cII repressor protein, BBa_C0053, binds to it. This segment contains O-R1, O-R2, a fragment of O-R3, the -35 of P-RM, and P-R (-10 and -35 from Tom Knight)
discontinued: false
dominant: false
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Maia Mahoney
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: OR2
sequence annotation: -35
sequence annotation: OR3
sequence annotation: OR1
sequence annotation: BBa_R0053
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R0053_sequence/1

OR2
type: SequenceAnnotation
persistent identity: annotation2036
display id: annotation2036
version: 1
top level: cII p22
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0053/annotation2036/range2036/1

https://synbiohub.org/public/igem/BBa_R0053/annotation2036/range2036/1
type: Range
persistent identity: range2036
display id: range2036
version: 1
top level: cII p22
direction: direction/forward
start: 11
end: 28
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2038
display id: annotation2038
version: 1
top level: cII p22
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0053/annotation2038/range2038/1

https://synbiohub.org/public/igem/BBa_R0053/annotation2038/range2038/1
type: Range
persistent identity: range2038
display id: range2038
version: 1
top level: cII p22
direction: direction/forward
start: 18
end: 23
orientation: inline

OR3
type: SequenceAnnotation
persistent identity: annotation2035
display id: annotation2035
version: 1
top level: cII p22
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0053/annotation2035/range2035/1

https://synbiohub.org/public/igem/BBa_R0053/annotation2035/range2035/1
type: Range
persistent identity: range2035
display id: range2035
version: 1
top level: cII p22
direction: direction/forward
start: 1
end: 3
orientation: inline

OR1
type: SequenceAnnotation
persistent identity: annotation2037
display id: annotation2037
version: 1
top level: cII p22
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0053/annotation2037/range2037/1

https://synbiohub.org/public/igem/BBa_R0053/annotation2037/range2037/1
type: Range
persistent identity: range2037
display id: range2037
version: 1
top level: cII p22
direction: direction/forward
start: 34
end: 51
orientation: inline

BBa_R0053
type: SequenceAnnotation
persistent identity: annotation7069
display id: annotation7069
version: 1
top level: cII p22
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_R0053/annotation7069/range7069/1

https://synbiohub.org/public/igem/BBa_R0053/annotation7069/range7069/1
type: Range
persistent identity: range7069
display id: range7069
version: 1
top level: cII p22
direction: direction/forward
start: 1
end: 54
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2042
display id: annotation2042
version: 1
top level: cII p22
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0053/annotation2042/range2042/1

https://synbiohub.org/public/igem/BBa_R0053/annotation2042/range2042/1
type: Range
persistent identity: range2042
display id: range2042
version: 1
top level: cII p22
direction: direction/forward
start: 30
end: 35
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2041
display id: annotation2041
version: 1
top level: cII p22
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0053/annotation2041/range2041/1

https://synbiohub.org/public/igem/BBa_R0053/annotation2041/range2041/1
type: Range
persistent identity: range2041
display id: range2041
version: 1
top level: cII p22
direction: direction/forward
start: 8
end: 13
orientation: inline

BBa_B0041
type: ComponentDefinition
persistent identity: BBa B0041
display id: BBa_B0041
version: 1
was generated by: iGEM to SBOL conversion
description: RBS 3
created: 2005-12-20T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Modified version of B0031 from the Voigt Lab. Not currently available as an independent part (only in a tet inverter).
discontinued: false
dominant: false
experience: experience/None
group u list: _11_
m user id: 0
owner id: 253
owning group id: 6
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Josh Michener
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_B0041_sequence/1

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

BBa_B0064
type: ComponentDefinition
persistent identity: BBa B0064
display id: BBa_B0064
version: 1
was generated by: iGEM to SBOL conversion
description: Tuned RBS for Q04401
created: 2008-02-23T12:00:00Z
modified: 2015-08-31T04:07:21Z
mutable provenance: .
owned by: james
owned by: myers
mutable description: This is a single bp change from B0034. It's strength is approximately 35% that of B0034.
discontinued: false
dominant: false
experience: experience/None
group u list: _11_
m user id: 0
owner id: 2
owning group id: 84
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: .
star: false
creator: Jason Kelly
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_B0064_sequence/1

cI857
type: ComponentDefinition
persistent identity: BBa J06503
display id: BBa_J06503
version: 1
was generated by: iGEM to SBOL conversion
description: Lambda cI857 (Lambda II) (RBS- LVA+)
created: 2005-06-27T11:00:00Z
modified: 2015-08-31T04:08:18Z
mutable provenance: Hendrix, Roger W. et al., ed. Lambda II. New York: Cold Spring Harbor Laboratory, 1983. ind- mutation on p. 626 (at bp 37589 of Lambda) 857 mutation on p. 628 (at bp 37742 of Lambda) Made by introducing two point mutations to Lambda cI (BBa_C0051): G(352)->A for ind- and G(199)->A for 857 temperature sensitivity.
owned by: james
owned by: myers
mutable description: Mutated from BBa_C0051 Repressor, Lambda cI (RBS- LVA+) to be temperature sensitive and UV insensitive.
discontinued: false
dominant: false
experience: experience/None
group u list: _20_
m user id: 0
owner id: 340
owning group id: 20
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: First attempt to mutate failed. Suspect the problem is a low mutation rate due to results of positive control. May be a result of the mutagenesis kit being old. Second attempt to mutate succeeded. However, the sequencing had a lot of bad sequence at the beginings, so that the ind- mutation was not sequenced by either of the primers used. The ind- mutation was verified by the presence of a HindIII restrition site. This part was never completely sequenced. The cI857 QPI was made by Blue Heron, and this was part was abandoned.
star: false
creator: ytwang
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: G->A (ind-)
sequence annotation: cI857
sequence annotation: barcode from LacI-LVA (BBa_C0012)
sequence annotation: G->A (857)
sequence annotation: LVA
sequence: https://synbiohub.org/public/igem/BBa_J06503_sequence/1

G->A (ind-)
type: SequenceAnnotation
persistent identity: annotation1549981
display id: annotation1549981
version: 1
top level: cI857
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_J06503/annotation1549981/range1549981/1

https://synbiohub.org/public/igem/BBa_J06503/annotation1549981/range1549981/1
type: Range
persistent identity: range1549981
display id: range1549981
version: 1
top level: cI857
direction: direction/forward
start: 352
end: 352
orientation: inline

cI857
type: SequenceAnnotation
persistent identity: annotation1549975
display id: annotation1549975
version: 1
top level: cI857
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J06503/annotation1549975/range1549975/1

https://synbiohub.org/public/igem/BBa_J06503/annotation1549975/range1549975/1
type: Range
persistent identity: range1549975
display id: range1549975
version: 1
top level: cI857
direction: direction/forward
start: 1
end: 711
orientation: inline

barcode from LacI-LVA (BBa_C0012)
type: SequenceAnnotation
persistent identity: annotation1552049
display id: annotation1552049
version: 1
top level: cI857
role: SO:0000807
role: feature/barcode
location: https://synbiohub.org/public/igem/BBa_J06503/annotation1552049/range1552049/1

https://synbiohub.org/public/igem/BBa_J06503/annotation1552049/range1552049/1
type: Range
persistent identity: range1552049
display id: range1552049
version: 1
top level: cI857
direction: direction/forward
start: 751
end: 775
orientation: inline

G->A (857)
type: SequenceAnnotation
persistent identity: annotation1549980
display id: annotation1549980
version: 1
top level: cI857
role: feature/mutation
role: SO:0001059
location: https://synbiohub.org/public/igem/BBa_J06503/annotation1549980/range1549980/1

https://synbiohub.org/public/igem/BBa_J06503/annotation1549980/range1549980/1
type: Range
persistent identity: range1549980
display id: range1549980
version: 1
top level: cI857
direction: direction/forward
start: 199
end: 199
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation1549979
display id: annotation1549979
version: 1
top level: cI857
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_J06503/annotation1549979/range1549979/1

https://synbiohub.org/public/igem/BBa_J06503/annotation1549979/range1549979/1
type: Range
persistent identity: range1549979
display id: range1549979
version: 1
top level: cI857
direction: direction/forward
start: 712
end: 744
orientation: inline

BBa_Q07400
type: ComponentDefinition
persistent identity: BBa Q07400
display id: BBa_Q07400
version: 1
was generated by: iGEM to SBOL conversion
description: tetR inverter, RBS3
created: 2005-12-20T12:00:00Z
modified: 2015-05-08T01:14:14Z
owned by: james
owned by: myers
mutable description: Inverter from the Voigt Lab
discontinued: false
dominant: false
group u list: _11_
m user id: 0
owner id: 253
owning group id: 6
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Josh Michener
type: DnaRegion
role: SO:0000804
role: partType/Inverter
component: BBa_C0040
component: BBa_B0041
component: BBa_B0012
component: BBa_R0040
component: BBa_B0010
sequence annotation: BBa_C0040
sequence annotation: BBa_B0041
sequence annotation: BBa_R0040
sequence annotation: BBa_B0012
sequence annotation: BBa_B0010
sequence: https://synbiohub.org/public/igem/BBa_Q07400_sequence/1

BBa_C0040
type: Component
persistent identity: component1771915
display id: component1771915
version: 1
top level: BBa_Q07400
access: public
definition: tetR

BBa_B0041
type: Component
persistent identity: component1771905
display id: component1771905
version: 1
top level: BBa_Q07400
access: public
definition: BBa_B0041

BBa_B0012
type: Component
persistent identity: component1771931
display id: component1771931
version: 1
top level: BBa_Q07400
access: public
definition: BBa_B0012

BBa_R0040
type: Component
persistent identity: component1771946
display id: component1771946
version: 1
top level: BBa_Q07400
access: public
definition: p(tetR)

BBa_B0010
type: Component
persistent identity: component1771921
display id: component1771921
version: 1
top level: BBa_Q07400
access: public
definition: BBa_B0010

BBa_C0040
type: SequenceAnnotation
persistent identity: annotation1771915
display id: annotation1771915
version: 1
top level: BBa_Q07400
location: https://synbiohub.org/public/igem/BBa_Q07400/annotation1771915/range1771915/1
component: BBa_C0040

https://synbiohub.org/public/igem/BBa_Q07400/annotation1771915/range1771915/1
type: Range
persistent identity: range1771915
display id: range1771915
version: 1
top level: BBa_Q07400
direction: direction/forward
start: 27
end: 686
orientation: inline

BBa_B0041
type: SequenceAnnotation
persistent identity: annotation1771905
display id: annotation1771905
version: 1
top level: BBa_Q07400
location: https://synbiohub.org/public/igem/BBa_Q07400/annotation1771905/range1771905/1
component: BBa_B0041

https://synbiohub.org/public/igem/BBa_Q07400/annotation1771905/range1771905/1
type: Range
persistent identity: range1771905
display id: range1771905
version: 1
top level: BBa_Q07400
direction: direction/forward
start: 1
end: 20
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1771946
display id: annotation1771946
version: 1
top level: BBa_Q07400
location: https://synbiohub.org/public/igem/BBa_Q07400/annotation1771946/range1771946/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_Q07400/annotation1771946/range1771946/1
type: Range
persistent identity: range1771946
display id: range1771946
version: 1
top level: BBa_Q07400
direction: direction/forward
start: 857
end: 910
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1771931
display id: annotation1771931
version: 1
top level: BBa_Q07400
location: https://synbiohub.org/public/igem/BBa_Q07400/annotation1771931/range1771931/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_Q07400/annotation1771931/range1771931/1
type: Range
persistent identity: range1771931
display id: range1771931
version: 1
top level: BBa_Q07400
direction: direction/forward
start: 808
end: 848
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1771921
display id: annotation1771921
version: 1
top level: BBa_Q07400
location: https://synbiohub.org/public/igem/BBa_Q07400/annotation1771921/range1771921/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_Q07400/annotation1771921/range1771921/1
type: Range
persistent identity: range1771921
display id: range1771921
version: 1
top level: BBa_Q07400
direction: direction/forward
start: 720
end: 799
orientation: inline

c2 P22
type: ComponentDefinition
persistent identity: BBa C0053
display id: BBa_C0053
version: 1
was generated by: iGEM to SBOL conversion
description: c2 repressor from Salmonella phage P22 (+LVA)
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:23Z
mutable provenance: Bacteriophage P22
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: The P22 c2 repressor protein coding sequence is a 720 base-pair sequence with the standard RBS-compatible BioBrick prefix and the standard BioBrick suffix sections on its ends. It binds to the P22 c2 regulatory sequence, BBa_R0053. The sequence contains a LVA tag for faster degredation.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: References (unparsed) here: Vander Byl,C. and Kropinski,A.M. Sequence of the genome of Salmonella bacteriophage P22 J. Bacteriol. 182 (22), 6472-6481 (2000) References (unparsed) here: Vander Byl,C. and Kropinski,A.M. Sequence of the genome of Salmonella bacteriophage P22 J. Bacteriol. 182 (22), 6472-6481 (2000)
star: true
creator: Maia Mahoney
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Help:Barcodes
sequence annotation: cII p22
sequence annotation: stop
sequence annotation: BBa_C0053
sequence annotation: LVA
sequence: https://synbiohub.org/public/igem/BBa_C0053_sequence/1

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213993
display id: annotation2213993
version: 1
top level: c2 P22
role: SO:0000807
role: feature/barcode
location: https://synbiohub.org/public/igem/BBa_C0053/annotation2213993/range2213993/1

https://synbiohub.org/public/igem/BBa_C0053/annotation2213993/range2213993/1
type: Range
persistent identity: range2213993
display id: range2213993
version: 1
top level: c2 P22
direction: direction/forward
start: 688
end: 712
orientation: inline

cII p22
type: SequenceAnnotation
persistent identity: annotation1747
display id: annotation1747
version: 1
top level: c2 P22
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_C0053/annotation1747/range1747/1

https://synbiohub.org/public/igem/BBa_C0053/annotation1747/range1747/1
type: Range
persistent identity: range1747
display id: range1747
version: 1
top level: c2 P22
direction: direction/forward
start: 1
end: 648
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1751
display id: annotation1751
version: 1
top level: c2 P22
role: feature/stop
role: SO:0000319
location: https://synbiohub.org/public/igem/BBa_C0053/annotation1751/range1751/1

https://synbiohub.org/public/igem/BBa_C0053/annotation1751/range1751/1
type: Range
persistent identity: range1751
display id: range1751
version: 1
top level: c2 P22
direction: direction/forward
start: 682
end: 687
orientation: inline

BBa_C0053
type: SequenceAnnotation
persistent identity: annotation7036
display id: annotation7036
version: 1
top level: c2 P22
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_C0053/annotation7036/range7036/1

https://synbiohub.org/public/igem/BBa_C0053/annotation7036/range7036/1
type: Range
persistent identity: range7036
display id: range7036
version: 1
top level: c2 P22
direction: direction/forward
start: 1
end: 687
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation1750
display id: annotation1750
version: 1
top level: c2 P22
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_C0053/annotation1750/range1750/1

https://synbiohub.org/public/igem/BBa_C0053/annotation1750/range1750/1
type: Range
persistent identity: range1750
display id: range1750
version: 1
top level: c2 P22
direction: direction/forward
start: 649
end: 681
orientation: inline

luxr
type: ComponentDefinition
persistent identity: BBa C0062
display id: BBa_C0062
version: 1
was generated by: iGEM to SBOL conversion
description: luxR repressor/activator, (no LVA?)
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:23Z
mutable provenance: V. fischeri <genbank>AF170104</genbank>
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: In complex with HSL, LuxR binds to the Lux promoter, activating transcription from Pr <bb_part>BBa_R0062</bb_part>, and repressing transcription from Pl <bb_part>BBa_R0063</bb_part>. The lux cassette of V. fischeri contains a left and a right promoter. The right promoter gives weak constitutive expression of downstream genes.This expression is up-regulated by the action of the Lux activator, LuxR complexed to HSL. Two molecules of LuxR protein form a complex with two molecules the signalling compound homoserine lactone (HSL). This complex binds to a palindromic site on the promoter, increasing the rate of transcription.
discontinued: false
dominant: true
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: 2 silent point mutants were introduced in the coding sequence to remove internal XbaI and PstI sites. Mutation sites were chosen to replace codons commonly used in E. coli with codons used at a similar frequency.
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Help:Barcodes
sequence annotation: prefix
sequence annotation: T
sequence annotation: luxR
sequence annotation: BBa_C0062
sequence annotation: A
sequence: https://synbiohub.org/public/igem/BBa_C0062_sequence/1

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213986
display id: annotation2213986
version: 1
top level: luxr
role: SO:0000807
role: feature/barcode
location: https://synbiohub.org/public/igem/BBa_C0062/annotation2213986/range2213986/1

https://synbiohub.org/public/igem/BBa_C0062/annotation2213986/range2213986/1
type: Range
persistent identity: range2213986
display id: range2213986
version: 1
top level: luxr
direction: direction/forward
start: 757
end: 781
orientation: inline

prefix
type: SequenceAnnotation
persistent identity: annotation1762
display id: annotation1762
version: 1
top level: luxr
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_C0062/annotation1762/range1762/1

https://synbiohub.org/public/igem/BBa_C0062/annotation1762/range1762/1
type: Range
persistent identity: range1762
display id: range1762
version: 1
top level: luxr
direction: direction/forward
start: 1
end: 2
orientation: inline

T
type: SequenceAnnotation
persistent identity: annotation1764
display id: annotation1764
version: 1
top level: luxr
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_C0062/annotation1764/range1764/1

https://synbiohub.org/public/igem/BBa_C0062/annotation1764/range1764/1
type: Range
persistent identity: range1764
display id: range1764
version: 1
top level: luxr
direction: direction/forward
start: 174
end: 174
orientation: inline

luxR
type: SequenceAnnotation
persistent identity: annotation1766
display id: annotation1766
version: 1
top level: luxr
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_C0062/annotation1766/range1766/1

https://synbiohub.org/public/igem/BBa_C0062/annotation1766/range1766/1
type: Range
persistent identity: range1766
display id: range1766
version: 1
top level: luxr
direction: direction/forward
start: 1
end: 750
orientation: inline

BBa_C0062
type: SequenceAnnotation
persistent identity: annotation7039
display id: annotation7039
version: 1
top level: luxr
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_C0062/annotation7039/range7039/1

https://synbiohub.org/public/igem/BBa_C0062/annotation7039/range7039/1
type: Range
persistent identity: range7039
display id: range7039
version: 1
top level: luxr
direction: direction/forward
start: 1
end: 756
orientation: inline

A
type: SequenceAnnotation
persistent identity: annotation1765
display id: annotation1765
version: 1
top level: luxr
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_C0062/annotation1765/range1765/1

https://synbiohub.org/public/igem/BBa_C0062/annotation1765/range1765/1
type: Range
persistent identity: range1765
display id: range1765
version: 1
top level: luxr
direction: direction/forward
start: 492
end: 492
orientation: inline

BBa_Q01121
type: ComponentDefinition
persistent identity: BBa Q01121
display id: BBa_Q01121
version: 1
was generated by: iGEM to SBOL conversion
description: QPI (B0031.C0012.B0015.R0011)
created: 2003-12-02T12:00:00Z
modified: 2015-05-08T01:14:13Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: LacI QPI w/ medium RBS, hybrid promoter
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Caitlin Conboy and Jennifer Braff
type: DnaRegion
role: SO:0000804
role: partType/Inverter
component: BBa_R0011
component: BBa_C0012
component: BBa_B0015
component: BBa_B0031
sequence annotation: BBa_R0011
sequence annotation: BBa_C0012
sequence annotation: BBa_B0015
sequence annotation: BBa_B0031
sequence: https://synbiohub.org/public/igem/BBa_Q01121_sequence/1

BBa_R0011
type: Component
persistent identity: component2221183
display id: component2221183
version: 1
top level: BBa_Q01121
access: public
definition: lacI+pL

BBa_C0012
type: Component
persistent identity: component2221173
display id: component2221173
version: 1
top level: BBa_Q01121
access: public
definition: lacI

BBa_B0015
type: Component
persistent identity: component2221182
display id: component2221182
version: 1
top level: BBa_Q01121
access: public
definition: BBa_B0015

BBa_B0031
type: Component
persistent identity: component2221171
display id: component2221171
version: 1
top level: BBa_Q01121
access: public
definition: BBa_B0031

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation2221183
display id: annotation2221183
version: 1
top level: BBa_Q01121
location: https://synbiohub.org/public/igem/BBa_Q01121/annotation2221183/range2221183/1
component: BBa_R0011

https://synbiohub.org/public/igem/BBa_Q01121/annotation2221183/range2221183/1
type: Range
persistent identity: range2221183
display id: range2221183
version: 1
top level: BBa_Q01121
direction: direction/forward
start: 1319
end: 1372
orientation: inline

BBa_C0012
type: SequenceAnnotation
persistent identity: annotation2221173
display id: annotation2221173
version: 1
top level: BBa_Q01121
location: https://synbiohub.org/public/igem/BBa_Q01121/annotation2221173/range2221173/1
component: BBa_C0012

https://synbiohub.org/public/igem/BBa_Q01121/annotation2221173/range2221173/1
type: Range
persistent identity: range2221173
display id: range2221173
version: 1
top level: BBa_Q01121
direction: direction/forward
start: 21
end: 1148
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2221182
display id: annotation2221182
version: 1
top level: BBa_Q01121
location: https://synbiohub.org/public/igem/BBa_Q01121/annotation2221182/range2221182/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_Q01121/annotation2221182/range2221182/1
type: Range
persistent identity: range2221182
display id: range2221182
version: 1
top level: BBa_Q01121
direction: direction/reverse
start: 1182
end: 1310
orientation: inline

BBa_B0031
type: SequenceAnnotation
persistent identity: annotation2221171
display id: annotation2221171
version: 1
top level: BBa_Q01121
location: https://synbiohub.org/public/igem/BBa_Q01121/annotation2221171/range2221171/1
component: BBa_B0031

https://synbiohub.org/public/igem/BBa_Q01121/annotation2221171/range2221171/1
type: Range
persistent identity: range2221171
display id: range2221171
version: 1
top level: BBa_Q01121
direction: direction/reverse
start: 1
end: 14
orientation: inline

Mnt
type: ComponentDefinition
persistent identity: BBa R0073
display id: BBa_R0073
version: 1
was generated by: iGEM to SBOL conversion
description: Promoter (Mnt regulated)
created: 2004-01-27T12:00:00Z
modified: 2015-05-08T01:14:15Z
mutable provenance: enterobacteriophage p22
owned by: james
owned by: myers
part status: Released HQ 2013
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: unsure of where -35 site is
star: true
creator: crackdots
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: dimer right half
sequence annotation: -10
sequence annotation: mnt
sequence annotation: dimer left half
sequence: https://synbiohub.org/public/igem/BBa_R0073_sequence/1

dimer right half
type: SequenceAnnotation
persistent identity: annotation318638
display id: annotation318638
version: 1
top level: Mnt
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0073/annotation318638/range318638/1

https://synbiohub.org/public/igem/BBa_R0073/annotation318638/range318638/1
type: Range
persistent identity: range318638
display id: range318638
version: 1
top level: Mnt
direction: direction/forward
start: 32
end: 38
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation318643
display id: annotation318643
version: 1
top level: Mnt
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0073/annotation318643/range318643/1

https://synbiohub.org/public/igem/BBa_R0073/annotation318643/range318643/1
type: Range
persistent identity: range318643
display id: range318643
version: 1
top level: Mnt
direction: direction/forward
start: 47
end: 52
orientation: inline

mnt
type: SequenceAnnotation
persistent identity: annotation302669
display id: annotation302669
version: 1
top level: Mnt
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_R0073/annotation302669/range302669/1

https://synbiohub.org/public/igem/BBa_R0073/annotation302669/range302669/1
type: Range
persistent identity: range302669
display id: range302669
version: 1
top level: Mnt
direction: direction/forward
start: 1
end: 67
orientation: inline

dimer left half
type: SequenceAnnotation
persistent identity: annotation318636
display id: annotation318636
version: 1
top level: Mnt
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0073/annotation318636/range318636/1

https://synbiohub.org/public/igem/BBa_R0073/annotation318636/range318636/1
type: Range
persistent identity: range318636
display id: range318636
version: 1
top level: Mnt
direction: direction/forward
start: 23
end: 29
orientation: inline

BBa_Q02400
type: ComponentDefinition
persistent identity: BBa Q02400
display id: BBa_Q02400
version: 1
was generated by: iGEM to SBOL conversion
description: Tet
created: 2004-07-19T11:00:00Z
modified: 2015-05-08T01:14:13Z
owned by: james
owned by: myers
mutable description: Tet QPI with B0032 which according to preliminary experiments is a "medium" strength RBS.
discontinued: false
dominant: false
experience: experience/None
group u list: _6_
m user id: 0
owner id: 101
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: mit
type: DnaRegion
role: SO:0000804
role: partType/Inverter
component: BBa_B0012
component: BBa_R0040
component: BBa_C0040
component: BBa_B0032
component: BBa_B0010
sequence annotation: BBa_B0032
sequence annotation: BBa_C0040
sequence annotation: BBa_B0012
sequence annotation: BBa_R0040
sequence annotation: BBa_B0010
sequence: https://synbiohub.org/public/igem/BBa_Q02400_sequence/1

BBa_B0012
type: Component
persistent identity: component952163
display id: component952163
version: 1
top level: BBa_Q02400
access: public
definition: BBa_B0012

BBa_R0040
type: Component
persistent identity: component952178
display id: component952178
version: 1
top level: BBa_Q02400
access: public
definition: p(tetR)

BBa_C0040
type: Component
persistent identity: component952147
display id: component952147
version: 1
top level: BBa_Q02400
access: public
definition: tetR

BBa_B0032
type: Component
persistent identity: component952136
display id: component952136
version: 1
top level: BBa_Q02400
access: public
definition: BBa_B0032

BBa_B0010
type: Component
persistent identity: component952153
display id: component952153
version: 1
top level: BBa_Q02400
access: public
definition: BBa_B0010

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation952136
display id: annotation952136
version: 1
top level: BBa_Q02400
location: https://synbiohub.org/public/igem/BBa_Q02400/annotation952136/range952136/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_Q02400/annotation952136/range952136/1
type: Range
persistent identity: range952136
display id: range952136
version: 1
top level: BBa_Q02400
direction: direction/forward
start: 1
end: 13
orientation: inline

BBa_C0040
type: SequenceAnnotation
persistent identity: annotation952147
display id: annotation952147
version: 1
top level: BBa_Q02400
location: https://synbiohub.org/public/igem/BBa_Q02400/annotation952147/range952147/1
component: BBa_C0040

https://synbiohub.org/public/igem/BBa_Q02400/annotation952147/range952147/1
type: Range
persistent identity: range952147
display id: range952147
version: 1
top level: BBa_Q02400
direction: direction/forward
start: 20
end: 679
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation952163
display id: annotation952163
version: 1
top level: BBa_Q02400
location: https://synbiohub.org/public/igem/BBa_Q02400/annotation952163/range952163/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_Q02400/annotation952163/range952163/1
type: Range
persistent identity: range952163
display id: range952163
version: 1
top level: BBa_Q02400
direction: direction/forward
start: 801
end: 841
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation952178
display id: annotation952178
version: 1
top level: BBa_Q02400
location: https://synbiohub.org/public/igem/BBa_Q02400/annotation952178/range952178/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_Q02400/annotation952178/range952178/1
type: Range
persistent identity: range952178
display id: range952178
version: 1
top level: BBa_Q02400
direction: direction/forward
start: 850
end: 903
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation952153
display id: annotation952153
version: 1
top level: BBa_Q02400
location: https://synbiohub.org/public/igem/BBa_Q02400/annotation952153/range952153/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_Q02400/annotation952153/range952153/1
type: Range
persistent identity: range952153
display id: range952153
version: 1
top level: BBa_Q02400
direction: direction/forward
start: 713
end: 792
orientation: inline

BBa_I9002
type: ComponentDefinition
persistent identity: BBa I9002
display id: BBa_I9002
version: 1
was generated by: iGEM to SBOL conversion
description: QPI (penI) B0034.C0074.B0015.R0074
created: 2004-01-27T12:00:00Z
modified: 2015-08-31T04:08:11Z
owned by: james
owned by: myers
discontinued: true
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: Discontinued
status: status/Deleted
bookmark: false
star: false
creator: crackdots
type: DnaRegion
role: SO:0000804
role: partType/Inverter
component: BBa_B0012
component: BBa_R0074
component: BBa_B0034
component: BBa_C0074
component: BBa_B0010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_B0034
sequence annotation: BBa_C0074
sequence annotation: BBa_R0074
sequence: https://synbiohub.org/public/igem/BBa_I9002_sequence/1

BBa_B0012
type: Component
persistent identity: component951185
display id: component951185
version: 1
top level: BBa_I9002
access: public
definition: BBa_B0012

BBa_R0074
type: Component
persistent identity: component951205
display id: component951205
version: 1
top level: BBa_I9002
access: public
definition: PenI

BBa_B0034
type: Component
persistent identity: component951159
display id: component951159
version: 1
top level: BBa_I9002
access: public
definition: BBa_B0034

BBa_C0074
type: Component
persistent identity: component951169
display id: component951169
version: 1
top level: BBa_I9002
access: public
definition: penI

BBa_B0010
type: Component
persistent identity: component951175
display id: component951175
version: 1
top level: BBa_I9002
access: public
definition: BBa_B0010

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation951175
display id: annotation951175
version: 1
top level: BBa_I9002
location: https://synbiohub.org/public/igem/BBa_I9002/annotation951175/range951175/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I9002/annotation951175/range951175/1
type: Range
persistent identity: range951175
display id: range951175
version: 1
top level: BBa_I9002
direction: direction/forward
start: 475
end: 554
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation951185
display id: annotation951185
version: 1
top level: BBa_I9002
location: https://synbiohub.org/public/igem/BBa_I9002/annotation951185/range951185/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I9002/annotation951185/range951185/1
type: Range
persistent identity: range951185
display id: range951185
version: 1
top level: BBa_I9002
direction: direction/forward
start: 563
end: 603
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation951159
display id: annotation951159
version: 1
top level: BBa_I9002
location: https://synbiohub.org/public/igem/BBa_I9002/annotation951159/range951159/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I9002/annotation951159/range951159/1
type: Range
persistent identity: range951159
display id: range951159
version: 1
top level: BBa_I9002
direction: direction/forward
start: 1
end: 12
orientation: inline

BBa_C0074
type: SequenceAnnotation
persistent identity: annotation951169
display id: annotation951169
version: 1
top level: BBa_I9002
location: https://synbiohub.org/public/igem/BBa_I9002/annotation951169/range951169/1
component: BBa_C0074

https://synbiohub.org/public/igem/BBa_I9002/annotation951169/range951169/1
type: Range
persistent identity: range951169
display id: range951169
version: 1
top level: BBa_I9002
direction: direction/forward
start: 19
end: 441
orientation: inline

BBa_R0074
type: SequenceAnnotation
persistent identity: annotation951205
display id: annotation951205
version: 1
top level: BBa_I9002
location: https://synbiohub.org/public/igem/BBa_I9002/annotation951205/range951205/1
component: BBa_R0074

https://synbiohub.org/public/igem/BBa_I9002/annotation951205/range951205/1
type: Range
persistent identity: range951205
display id: range951205
version: 1
top level: BBa_I9002
direction: direction/forward
start: 612
end: 688
orientation: inline

BBa_Q09400
type: ComponentDefinition
persistent identity: BBa Q09400
display id: BBa_Q09400
version: 1
was generated by: iGEM to SBOL conversion
description: tetR inverter, RBS2
created: 2005-12-20T12:00:00Z
modified: 2015-05-08T01:14:14Z
owned by: james
owned by: myers
mutable description: Inverter from the Voigt Lab
discontinued: false
dominant: false
group u list: _11_
m user id: 0
owner id: 253
owning group id: 6
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Josh Michener
type: DnaRegion
role: SO:0000804
role: partType/Inverter
component: BBa_B0010
component: BBa_B0039
component: BBa_B0012
component: BBa_R0040
component: BBa_C0040
sequence annotation: BBa_C0040
sequence annotation: BBa_B0012
sequence annotation: BBa_B0039
sequence annotation: BBa_B0010
sequence annotation: BBa_R0040
sequence: https://synbiohub.org/public/igem/BBa_Q09400_sequence/1

BBa_B0010
type: Component
persistent identity: component1771849
display id: component1771849
version: 1
top level: BBa_Q09400
access: public
definition: BBa_B0010

BBa_B0039
type: Component
persistent identity: component1771833
display id: component1771833
version: 1
top level: BBa_Q09400
access: public
definition: BBa_B0039

BBa_B0012
type: Component
persistent identity: component1771859
display id: component1771859
version: 1
top level: BBa_Q09400
access: public
definition: BBa_B0012

BBa_R0040
type: Component
persistent identity: component1771874
display id: component1771874
version: 1
top level: BBa_Q09400
access: public
definition: p(tetR)

BBa_C0040
type: Component
persistent identity: component1771843
display id: component1771843
version: 1
top level: BBa_Q09400
access: public
definition: tetR

BBa_C0040
type: SequenceAnnotation
persistent identity: annotation1771843
display id: annotation1771843
version: 1
top level: BBa_Q09400
location: https://synbiohub.org/public/igem/BBa_Q09400/annotation1771843/range1771843/1
component: BBa_C0040

https://synbiohub.org/public/igem/BBa_Q09400/annotation1771843/range1771843/1
type: Range
persistent identity: range1771843
display id: range1771843
version: 1
top level: BBa_Q09400
direction: direction/forward
start: 29
end: 688
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1771859
display id: annotation1771859
version: 1
top level: BBa_Q09400
location: https://synbiohub.org/public/igem/BBa_Q09400/annotation1771859/range1771859/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_Q09400/annotation1771859/range1771859/1
type: Range
persistent identity: range1771859
display id: range1771859
version: 1
top level: BBa_Q09400
direction: direction/forward
start: 810
end: 850
orientation: inline

BBa_B0039
type: SequenceAnnotation
persistent identity: annotation1771833
display id: annotation1771833
version: 1
top level: BBa_Q09400
location: https://synbiohub.org/public/igem/BBa_Q09400/annotation1771833/range1771833/1
component: BBa_B0039

https://synbiohub.org/public/igem/BBa_Q09400/annotation1771833/range1771833/1
type: Range
persistent identity: range1771833
display id: range1771833
version: 1
top level: BBa_Q09400
direction: direction/forward
start: 1
end: 22
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1771849
display id: annotation1771849
version: 1
top level: BBa_Q09400
location: https://synbiohub.org/public/igem/BBa_Q09400/annotation1771849/range1771849/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_Q09400/annotation1771849/range1771849/1
type: Range
persistent identity: range1771849
display id: range1771849
version: 1
top level: BBa_Q09400
direction: direction/forward
start: 722
end: 801
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1771874
display id: annotation1771874
version: 1
top level: BBa_Q09400
location: https://synbiohub.org/public/igem/BBa_Q09400/annotation1771874/range1771874/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_Q09400/annotation1771874/range1771874/1
type: Range
persistent identity: range1771874
display id: range1771874
version: 1
top level: BBa_Q09400
direction: direction/forward
start: 859
end: 912
orientation: inline

BBa_Q04740
type: ComponentDefinition
persistent identity: BBa Q04740
display id: BBa_Q04740
version: 1
was generated by: iGEM to SBOL conversion
description: Pen1 quad part inverter
created: 2004-07-04T11:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: Crackdots
owned by: james
owned by: myers
mutable description: -- No description --
discontinued: false
dominant: false
experience: experience/None
group u list: _11_6_
m user id: 0
owner id: 135
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Barry Canton
type: DnaRegion
role: SO:0000804
role: partType/Inverter
component: BBa_R0074
component: BBa_C0074
component: BBa_B0034
component: BBa_B0010
component: BBa_B0012
sequence annotation: BBa_B0034
sequence annotation: BBa_B0012
sequence annotation: BBa_C0074
sequence annotation: BBa_B0010
sequence annotation: BBa_R0074
sequence: https://synbiohub.org/public/igem/BBa_Q04740_sequence/1

BBa_R0074
type: Component
persistent identity: component953148
display id: component953148
version: 1
top level: BBa_Q04740
access: public
definition: PenI

BBa_C0074
type: Component
persistent identity: component953112
display id: component953112
version: 1
top level: BBa_Q04740
access: public
definition: penI

BBa_B0034
type: Component
persistent identity: component953102
display id: component953102
version: 1
top level: BBa_Q04740
access: public
definition: BBa_B0034

BBa_B0010
type: Component
persistent identity: component953118
display id: component953118
version: 1
top level: BBa_Q04740
access: public
definition: BBa_B0010

BBa_B0012
type: Component
persistent identity: component953128
display id: component953128
version: 1
top level: BBa_Q04740
access: public
definition: BBa_B0012

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation953102
display id: annotation953102
version: 1
top level: BBa_Q04740
location: https://synbiohub.org/public/igem/BBa_Q04740/annotation953102/range953102/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_Q04740/annotation953102/range953102/1
type: Range
persistent identity: range953102
display id: range953102
version: 1
top level: BBa_Q04740
direction: direction/forward
start: 1
end: 12
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation953128
display id: annotation953128
version: 1
top level: BBa_Q04740
location: https://synbiohub.org/public/igem/BBa_Q04740/annotation953128/range953128/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_Q04740/annotation953128/range953128/1
type: Range
persistent identity: range953128
display id: range953128
version: 1
top level: BBa_Q04740
direction: direction/forward
start: 563
end: 603
orientation: inline

BBa_C0074
type: SequenceAnnotation
persistent identity: annotation953112
display id: annotation953112
version: 1
top level: BBa_Q04740
location: https://synbiohub.org/public/igem/BBa_Q04740/annotation953112/range953112/1
component: BBa_C0074

https://synbiohub.org/public/igem/BBa_Q04740/annotation953112/range953112/1
type: Range
persistent identity: range953112
display id: range953112
version: 1
top level: BBa_Q04740
direction: direction/forward
start: 19
end: 441
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation953118
display id: annotation953118
version: 1
top level: BBa_Q04740
location: https://synbiohub.org/public/igem/BBa_Q04740/annotation953118/range953118/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_Q04740/annotation953118/range953118/1
type: Range
persistent identity: range953118
display id: range953118
version: 1
top level: BBa_Q04740
direction: direction/forward
start: 475
end: 554
orientation: inline

BBa_R0074
type: SequenceAnnotation
persistent identity: annotation953148
display id: annotation953148
version: 1
top level: BBa_Q04740
location: https://synbiohub.org/public/igem/BBa_Q04740/annotation953148/range953148/1
component: BBa_R0074

https://synbiohub.org/public/igem/BBa_Q04740/annotation953148/range953148/1
type: Range
persistent identity: range953148
display id: range953148
version: 1
top level: BBa_Q04740
direction: direction/forward
start: 612
end: 688
orientation: inline

lacI ts
type: ComponentDefinition
persistent identity: BBa J06500
display id: BBa_J06500
version: 1
was generated by: iGEM to SBOL conversion
description: LacI repressor (temperature-sensitive mut 241) (+LVA)
created: 2005-06-13T11:00:00Z
modified: 2015-08-31T04:08:18Z
owned by: james
owned by: myers
mutable description: Mutated from BBa_C0012 Repressor, LacI (LVA+) to be temperature sensitive. Mutation from Yabuta, 1995: Ala241->Thr (G(730)CG->ACG)
discontinued: false
dominant: false
experience: experience/None
group u list: _20_
m user id: 0
owner id: 340
owning group id: 20
sample status: It's complicated
status: status/Planning
bookmark: false
star: false
creator: ytwang
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: barcode from LacI (BBa_C0012)
sequence annotation: LacI(ts)-LVA
sequence annotation: TS mutation (G->A)
sequence annotation: LVA
sequence: https://synbiohub.org/public/igem/BBa_J06500_sequence/1

barcode from LacI (BBa_C0012)
type: SequenceAnnotation
persistent identity: annotation1518229
display id: annotation1518229
version: 1
top level: lacI ts
role: SO:0000807
role: feature/barcode
location: https://synbiohub.org/public/igem/BBa_J06500/annotation1518229/range1518229/1

https://synbiohub.org/public/igem/BBa_J06500/annotation1518229/range1518229/1
type: Range
persistent identity: range1518229
display id: range1518229
version: 1
top level: lacI ts
direction: direction/forward
start: 1129
end: 1153
orientation: inline

LacI(ts)-LVA
type: SequenceAnnotation
persistent identity: annotation1518213
display id: annotation1518213
version: 1
top level: lacI ts
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J06500/annotation1518213/range1518213/1

https://synbiohub.org/public/igem/BBa_J06500/annotation1518213/range1518213/1
type: Range
persistent identity: range1518213
display id: range1518213
version: 1
top level: lacI ts
direction: direction/forward
start: 1
end: 1128
orientation: inline

TS mutation (G->A)
type: SequenceAnnotation
persistent identity: annotation1518230
display id: annotation1518230
version: 1
top level: lacI ts
role: feature/mutation
role: SO:0001059
location: https://synbiohub.org/public/igem/BBa_J06500/annotation1518230/range1518230/1

https://synbiohub.org/public/igem/BBa_J06500/annotation1518230/range1518230/1
type: Range
persistent identity: range1518230
display id: range1518230
version: 1
top level: lacI ts
direction: direction/forward
start: 730
end: 730
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation1518220
display id: annotation1518220
version: 1
top level: lacI ts
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_J06500/annotation1518220/range1518220/1

https://synbiohub.org/public/igem/BBa_J06500/annotation1518220/range1518220/1
type: Range
persistent identity: range1518220
display id: range1518220
version: 1
top level: lacI ts
direction: direction/forward
start: 1090
end: 1128
orientation: inline

BBa_Q04530
type: ComponentDefinition
persistent identity: BBa Q04530
display id: BBa_Q04530
version: 1
was generated by: iGEM to SBOL conversion
description: QPI (B0034.C0053.B0015.R0053)
created: 2003-12-02T12:00:00Z
modified: 2015-05-08T01:14:14Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: p22 c2 QPI w/ strong RBS
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: false
creator: Caitlin Conboy and Jennifer Braff
type: DnaRegion
role: SO:0000804
role: partType/Inverter
component: BBa_R0053
component: BBa_B0012
component: BBa_B0010
component: BBa_C0053
component: BBa_B0034
sequence annotation: BBa_R0053
sequence annotation: BBa_B0012
sequence annotation: BBa_B0010
sequence annotation: BBa_C0053
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_Q04530_sequence/1

BBa_R0053
type: Component
persistent identity: component953094
display id: component953094
version: 1
top level: BBa_Q04530
access: public
definition: cII p22

BBa_B0012
type: Component
persistent identity: component953075
display id: component953075
version: 1
top level: BBa_Q04530
access: public
definition: BBa_B0012

BBa_B0010
type: Component
persistent identity: component953065
display id: component953065
version: 1
top level: BBa_Q04530
access: public
definition: BBa_B0010

BBa_C0053
type: Component
persistent identity: component953057
display id: component953057
version: 1
top level: BBa_Q04530
access: public
definition: c2 P22

BBa_B0034
type: Component
persistent identity: component953045
display id: component953045
version: 1
top level: BBa_Q04530
access: public
definition: BBa_B0034

BBa_R0053
type: SequenceAnnotation
persistent identity: annotation953094
display id: annotation953094
version: 1
top level: BBa_Q04530
location: https://synbiohub.org/public/igem/BBa_Q04530/annotation953094/range953094/1
component: BBa_R0053

https://synbiohub.org/public/igem/BBa_Q04530/annotation953094/range953094/1
type: Range
persistent identity: range953094
display id: range953094
version: 1
top level: BBa_Q04530
direction: direction/forward
start: 876
end: 929
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation953075
display id: annotation953075
version: 1
top level: BBa_Q04530
location: https://synbiohub.org/public/igem/BBa_Q04530/annotation953075/range953075/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_Q04530/annotation953075/range953075/1
type: Range
persistent identity: range953075
display id: range953075
version: 1
top level: BBa_Q04530
direction: direction/forward
start: 827
end: 867
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation953065
display id: annotation953065
version: 1
top level: BBa_Q04530
location: https://synbiohub.org/public/igem/BBa_Q04530/annotation953065/range953065/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_Q04530/annotation953065/range953065/1
type: Range
persistent identity: range953065
display id: range953065
version: 1
top level: BBa_Q04530
direction: direction/forward
start: 739
end: 818
orientation: inline

BBa_C0053
type: SequenceAnnotation
persistent identity: annotation953057
display id: annotation953057
version: 1
top level: BBa_Q04530
location: https://synbiohub.org/public/igem/BBa_Q04530/annotation953057/range953057/1
component: BBa_C0053

https://synbiohub.org/public/igem/BBa_Q04530/annotation953057/range953057/1
type: Range
persistent identity: range953057
display id: range953057
version: 1
top level: BBa_Q04530
direction: direction/forward
start: 19
end: 705
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation953045
display id: annotation953045
version: 1
top level: BBa_Q04530
location: https://synbiohub.org/public/igem/BBa_Q04530/annotation953045/range953045/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_Q04530/annotation953045/range953045/1
type: Range
persistent identity: range953045
display id: range953045
version: 1
top level: BBa_Q04530
direction: direction/forward
start: 1
end: 12
orientation: inline

cI lam
type: ComponentDefinition
persistent identity: BBa C0051
display id: BBa_C0051
version: 1
was generated by: iGEM to SBOL conversion
description: cI repressor from E. coli phage lambda (+LVA)
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:23Z
mutable provenance: Elowitz, M. B. Transport, Assembly, and Dynamics in Systems of Interacting Proteins. Thesis, Princeton Univ., Princeton (1999).
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Coding region for the cI repressor based on cI repressor from bacteriophage lambda modified with an LVA tail for rapid degradation of the protein. cI repressor binds to the cI regulator (BBa_R0051).
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: References (unparsed) here: A synthetic oscillatory network of transcriptional regulators , Elowitz M.B. , Leibler S., Nature(403),335-38: 2000 Octamerization of CI repressor is needed for effective repression of PRM and efficient switching from lysogeny. Ian B. Dodd,1 Alison J. Perkins, Daniel Tsemitsidis, and J. Barry Egan , Genes and Development (Vol 15, No. 22) 3013-3022: 2001 References (unparsed) here: A synthetic oscillatory network of transcriptional regulators , Elowitz M.B. , Leibler S., Nature(403),335-38: 2000 Octamerization of CI repressor is needed for effective repression of PRM and efficient switching from lysogeny. Ian B. Dodd,1 Alison J. Perkins, Daniel Tsemitsidis, and J. Barry Egan , Genes and Development (Vol 15, No. 22) 3013-3022: 2001 BBa_C0051 cI repressor is based on the cI repressor from the Elowitz's repressilator. It has been modified to include a rapid degradation LAA tail, and includes the BioBrick standard assembly head and tail restriction sites. The RBS has been removed. The stop codon has been changed from TAA to a double stop codon TAATAA.
star: true
creator: Vinay S Mahajan, Brian Chow, Peter Carr, Voichita Marinescu and Alexander D. Wissner-Gross
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Help:Barcodes
sequence annotation: cI lambda
sequence annotation: LVA
sequence: https://synbiohub.org/public/igem/BBa_C0051_sequence/1

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213991
display id: annotation2213991
version: 1
top level: cI lam
role: SO:0000807
role: feature/barcode
location: https://synbiohub.org/public/igem/BBa_C0051/annotation2213991/range2213991/1

https://synbiohub.org/public/igem/BBa_C0051/annotation2213991/range2213991/1
type: Range
persistent identity: range2213991
display id: range2213991
version: 1
top level: cI lam
direction: direction/forward
start: 751
end: 775
orientation: inline

cI lambda
type: SequenceAnnotation
persistent identity: annotation23334
display id: annotation23334
version: 1
top level: cI lam
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_C0051/annotation23334/range23334/1

https://synbiohub.org/public/igem/BBa_C0051/annotation23334/range23334/1
type: Range
persistent identity: range23334
display id: range23334
version: 1
top level: cI lam
direction: direction/forward
start: 4
end: 711
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation23335
display id: annotation23335
version: 1
top level: cI lam
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_C0051/annotation23335/range23335/1

https://synbiohub.org/public/igem/BBa_C0051/annotation23335/range23335/1
type: Range
persistent identity: range23335
display id: range23335
version: 1
top level: cI lam
direction: direction/forward
start: 712
end: 744
orientation: inline

BBa_K1366002
type: ComponentDefinition
persistent identity: BBa K1366002
display id: BBa_K1366002
version: 1
was generated by: iGEM to SBOL conversion
description: Genetic construct for lpp gen deletion
created: 2014-10-09T11:00:00Z
modified: 2015-05-08T01:10:07Z
mutable provenance: The genetic design is based in the lpp deletion done by: Ni, Y., Reyes, J., & Chen, R. (2007). lpp Deletion as a Permeabilization Method. Biotechnology and Bioengineering, 1347-1356. It also contains some parts of the registry like BBa_K1073000, BBa_B0034, BBa_B0015
owned by: james
owned by: myers
mutable description: This biobrick contains the sequence homologies (50bp) to delete lpp gen (LPS moiety carrier) in E. coli with an ampicillin resistance and a Multiple Cloning Site (for the generation of genomic knock-ins of any desired gen in E. coli). The deletion of lpp gene and the replacement of that sequence with the Amp resistance. Gene deletions are performed with lambda-red recombination technology. FLP-FRT sequences are included to remove the antibiotic resistance with a specific recombinase.
discontinued: false
dominant: false
group u list: _1743_
m user id: 0
owner id: 16722
owning group id: 9
sample status: No part sequence
status: status/Planning
bookmark: false
mutable notes: This is the general structure 50bp homology Lpp // FRT // Amp R Promoter // RBS // AmpR // Terminator // FRT // MCS // 50 bp homology Lpp
star: false
creator: Eduardo Cepeda Ca??edo
type: DnaRegion
role: SO:0000804
role: partType/Composite

BBa_I9004
type: ComponentDefinition
persistent identity: BBa I9004
display id: BBa_I9004
version: 1
was generated by: iGEM to SBOL conversion
description: QPI (lambda-cI) B0034.I9200.B0015.I9201
created: 2004-01-27T12:00:00Z
modified: 2015-08-31T04:08:11Z
owned by: james
owned by: myers
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: crackdots
type: DnaRegion
role: SO:0000804
role: partType/Inverter
component: BBa_B0012
component: BBa_I9200
component: BBa_B0010
component: BBa_I9201
component: BBa_B0034
sequence annotation: BBa_B0034
sequence annotation: BBa_B0010
sequence annotation: BBa_I9200
sequence annotation: BBa_I9201
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_I9004_sequence/1

BBa_B0012
type: Component
persistent identity: component951280
display id: component951280
version: 1
top level: BBa_I9004
access: public
definition: BBa_B0012

BBa_I9200
type: Component
persistent identity: component951264
display id: component951264
version: 1
top level: BBa_I9004
access: public
definition: cI

BBa_B0010
type: Component
persistent identity: component951270
display id: component951270
version: 1
top level: BBa_I9004
access: public
definition: BBa_B0010

BBa_I9201
type: Component
persistent identity: component951288
display id: component951288
version: 1
top level: BBa_I9004
access: public
definition: cI lam

BBa_B0034
type: Component
persistent identity: component951257
display id: component951257
version: 1
top level: BBa_I9004
access: public
definition: BBa_B0034

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation951257
display id: annotation951257
version: 1
top level: BBa_I9004
location: https://synbiohub.org/public/igem/BBa_I9004/annotation951257/range951257/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I9004/annotation951257/range951257/1
type: Range
persistent identity: range951257
display id: range951257
version: 1
top level: BBa_I9004
direction: direction/forward
start: 1
end: 12
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation951270
display id: annotation951270
version: 1
top level: BBa_I9004
location: https://synbiohub.org/public/igem/BBa_I9004/annotation951270/range951270/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I9004/annotation951270/range951270/1
type: Range
persistent identity: range951270
display id: range951270
version: 1
top level: BBa_I9004
direction: direction/forward
start: 769
end: 848
orientation: inline

BBa_I9200
type: SequenceAnnotation
persistent identity: annotation951264
display id: annotation951264
version: 1
top level: BBa_I9004
location: https://synbiohub.org/public/igem/BBa_I9004/annotation951264/range951264/1
component: BBa_I9200

https://synbiohub.org/public/igem/BBa_I9004/annotation951264/range951264/1
type: Range
persistent identity: range951264
display id: range951264
version: 1
top level: BBa_I9004
direction: direction/forward
start: 19
end: 735
orientation: inline

BBa_I9201
type: SequenceAnnotation
persistent identity: annotation951288
display id: annotation951288
version: 1
top level: BBa_I9004
location: https://synbiohub.org/public/igem/BBa_I9004/annotation951288/range951288/1
component: BBa_I9201

https://synbiohub.org/public/igem/BBa_I9004/annotation951288/range951288/1
type: Range
persistent identity: range951288
display id: range951288
version: 1
top level: BBa_I9004
direction: direction/forward
start: 906
end: 987
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation951280
display id: annotation951280
version: 1
top level: BBa_I9004
location: https://synbiohub.org/public/igem/BBa_I9004/annotation951280/range951280/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I9004/annotation951280/range951280/1
type: Range
persistent identity: range951280
display id: range951280
version: 1
top level: BBa_I9004
direction: direction/forward
start: 857
end: 897
orientation: inline

BBa_B0038
type: ComponentDefinition
persistent identity: BBa B0038
display id: BBa_B0038
version: 1
was generated by: iGEM to SBOL conversion
description: RBS 1
created: 2005-12-20T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Modified version of B0031 from the Voigt Lab. Not currently available as an independent part (only in a tet inverter).
discontinued: false
dominant: false
experience: experience/None
group u list: _11_
m user id: 0
owner id: 253
owning group id: 6
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Josh Michener
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_B0038_sequence/1

BBa_J06800
type: ComponentDefinition
persistent identity: BBa J06800
display id: BBa_J06800
version: 1
was generated by: iGEM to SBOL conversion
description: QPI (B0034.J06500.B0015.R0011)
created: 2005-06-13T11:00:00Z
modified: 2015-08-31T04:08:18Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: LacI (ts) QPI w/ strong RBS
discontinued: false
dominant: false
experience: experience/None
group u list: _20_
m user id: 0
owner id: 340
owning group id: 20
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Mutation has been sequenced. The rest of the part will be sequenced shortly.
star: true
creator: ytwang
type: DnaRegion
role: SO:0000804
role: partType/Inverter
component: BBa_B0034
component: BBa_R0011
component: BBa_J06500
component: BBa_B0010
component: BBa_B0012
sequence annotation: BBa_B0010
sequence annotation: BBa_R0011
sequence annotation: BBa_B0034
sequence annotation: BBa_B0012
sequence annotation: BBa_J06500
sequence: https://synbiohub.org/public/igem/BBa_J06800_sequence/1

BBa_B0034
type: Component
persistent identity: component1531229
display id: component1531229
version: 1
top level: BBa_J06800
access: public
definition: BBa_B0034

BBa_R0011
type: Component
persistent identity: component1531272
display id: component1531272
version: 1
top level: BBa_J06800
access: public
definition: lacI+pL

BBa_J06500
type: Component
persistent identity: component1531243
display id: component1531243
version: 1
top level: BBa_J06800
access: public
definition: lacI ts

BBa_B0010
type: Component
persistent identity: component1531248
display id: component1531248
version: 1
top level: BBa_J06800
access: public
definition: BBa_B0010

BBa_B0012
type: Component
persistent identity: component1531258
display id: component1531258
version: 1
top level: BBa_J06800
access: public
definition: BBa_B0012

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1531248
display id: annotation1531248
version: 1
top level: BBa_J06800
location: https://synbiohub.org/public/igem/BBa_J06800/annotation1531248/range1531248/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_J06800/annotation1531248/range1531248/1
type: Range
persistent identity: range1531248
display id: range1531248
version: 1
top level: BBa_J06800
direction: direction/forward
start: 1180
end: 1259
orientation: inline

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation1531272
display id: annotation1531272
version: 1
top level: BBa_J06800
location: https://synbiohub.org/public/igem/BBa_J06800/annotation1531272/range1531272/1
component: BBa_R0011

https://synbiohub.org/public/igem/BBa_J06800/annotation1531272/range1531272/1
type: Range
persistent identity: range1531272
display id: range1531272
version: 1
top level: BBa_J06800
direction: direction/forward
start: 1317
end: 1370
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1531229
display id: annotation1531229
version: 1
top level: BBa_J06800
location: https://synbiohub.org/public/igem/BBa_J06800/annotation1531229/range1531229/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_J06800/annotation1531229/range1531229/1
type: Range
persistent identity: range1531229
display id: range1531229
version: 1
top level: BBa_J06800
direction: direction/forward
start: 1
end: 12
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1531258
display id: annotation1531258
version: 1
top level: BBa_J06800
location: https://synbiohub.org/public/igem/BBa_J06800/annotation1531258/range1531258/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_J06800/annotation1531258/range1531258/1
type: Range
persistent identity: range1531258
display id: range1531258
version: 1
top level: BBa_J06800
direction: direction/forward
start: 1268
end: 1308
orientation: inline

BBa_J06500
type: SequenceAnnotation
persistent identity: annotation1531243
display id: annotation1531243
version: 1
top level: BBa_J06800
location: https://synbiohub.org/public/igem/BBa_J06800/annotation1531243/range1531243/1
component: BBa_J06500

https://synbiohub.org/public/igem/BBa_J06800/annotation1531243/range1531243/1
type: Range
persistent identity: range1531243
display id: range1531243
version: 1
top level: BBa_J06800
direction: direction/forward
start: 19
end: 1171
orientation: inline

lacI ts
type: ComponentDefinition
persistent identity: BBa J06501
display id: BBa_J06501
version: 1
was generated by: iGEM to SBOL conversion
description: LacI repressor (temperature-sensitive mut 265) (+LVA)
created: 2005-06-13T11:00:00Z
modified: 2015-08-31T04:08:18Z
owned by: james
owned by: myers
mutable description: Mutated from BBa_C0012 Repressor, LacI (LVA+) to be temperature sensitive. Mutation from Chao, et al., 2002: Gly265->Asp (GG(803)T->GAT)
discontinued: false
dominant: false
experience: experience/None
group u list: _20_
m user id: 0
owner id: 340
owning group id: 20
sample status: It's complicated
status: status/Planning
bookmark: false
star: false
creator: ytwang
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: LacI(ts)-LVA
sequence annotation: barcode from LacI (BBa_C0012)
sequence annotation: LVA
sequence annotation: TS mutation (G->A)
sequence: https://synbiohub.org/public/igem/BBa_J06501_sequence/1

LacI(ts)-LVA
type: SequenceAnnotation
persistent identity: annotation1530899
display id: annotation1530899
version: 1
top level: lacI ts
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J06501/annotation1530899/range1530899/1

https://synbiohub.org/public/igem/BBa_J06501/annotation1530899/range1530899/1
type: Range
persistent identity: range1530899
display id: range1530899
version: 1
top level: lacI ts
direction: direction/forward
start: 1
end: 1128
orientation: inline

barcode from LacI (BBa_C0012)
type: SequenceAnnotation
persistent identity: annotation1530914
display id: annotation1530914
version: 1
top level: lacI ts
role: SO:0000807
role: feature/barcode
location: https://synbiohub.org/public/igem/BBa_J06501/annotation1530914/range1530914/1

https://synbiohub.org/public/igem/BBa_J06501/annotation1530914/range1530914/1
type: Range
persistent identity: range1530914
display id: range1530914
version: 1
top level: lacI ts
direction: direction/forward
start: 1129
end: 1153
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation1530907
display id: annotation1530907
version: 1
top level: lacI ts
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_J06501/annotation1530907/range1530907/1

https://synbiohub.org/public/igem/BBa_J06501/annotation1530907/range1530907/1
type: Range
persistent identity: range1530907
display id: range1530907
version: 1
top level: lacI ts
direction: direction/forward
start: 1090
end: 1128
orientation: inline

TS mutation (G->A)
type: SequenceAnnotation
persistent identity: annotation1530900
display id: annotation1530900
version: 1
top level: lacI ts
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_J06501/annotation1530900/range1530900/1

https://synbiohub.org/public/igem/BBa_J06501/annotation1530900/range1530900/1
type: Range
persistent identity: range1530900
display id: range1530900
version: 1
top level: lacI ts
direction: direction/forward
start: 803
end: 803
orientation: inline

BBa_Q01122
type: ComponentDefinition
persistent identity: BBa Q01122
display id: BBa_Q01122
version: 1
was generated by: iGEM to SBOL conversion
description: QPI (B0031.C0012.B0015.R0010)
created: 2003-12-02T12:00:00Z
modified: 2015-05-08T01:14:13Z
owned by: james
owned by: myers
mutable description: LacI QPI w/medium RBS
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Caitlin Conboy and Jennifer Braff
type: DnaRegion
role: SO:0000804
role: partType/Inverter
component: BBa_B0010
component: BBa_R0010
component: BBa_B0012
component: BBa_B0031
component: BBa_C0012
sequence annotation: BBa_B0031
sequence annotation: BBa_C0012
sequence annotation: BBa_B0010
sequence annotation: BBa_R0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_Q01122_sequence/1

BBa_B0010
type: Component
persistent identity: component951728
display id: component951728
version: 1
top level: BBa_Q01122
access: public
definition: BBa_B0010

BBa_R0010
type: Component
persistent identity: component951754
display id: component951754
version: 1
top level: BBa_Q01122
access: public
definition: LacI

BBa_B0012
type: Component
persistent identity: component951738
display id: component951738
version: 1
top level: BBa_Q01122
access: public
definition: BBa_B0012

BBa_B0031
type: Component
persistent identity: component951711
display id: component951711
version: 1
top level: BBa_Q01122
access: public
definition: BBa_B0031

BBa_C0012
type: Component
persistent identity: component951721
display id: component951721
version: 1
top level: BBa_Q01122
access: public
definition: lacI

BBa_B0031
type: SequenceAnnotation
persistent identity: annotation951711
display id: annotation951711
version: 1
top level: BBa_Q01122
location: https://synbiohub.org/public/igem/BBa_Q01122/annotation951711/range951711/1
component: BBa_B0031

https://synbiohub.org/public/igem/BBa_Q01122/annotation951711/range951711/1
type: Range
persistent identity: range951711
display id: range951711
version: 1
top level: BBa_Q01122
direction: direction/forward
start: 1
end: 14
orientation: inline

BBa_C0012
type: SequenceAnnotation
persistent identity: annotation951721
display id: annotation951721
version: 1
top level: BBa_Q01122
location: https://synbiohub.org/public/igem/BBa_Q01122/annotation951721/range951721/1
component: BBa_C0012

https://synbiohub.org/public/igem/BBa_Q01122/annotation951721/range951721/1
type: Range
persistent identity: range951721
display id: range951721
version: 1
top level: BBa_Q01122
direction: direction/forward
start: 21
end: 1148
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation951728
display id: annotation951728
version: 1
top level: BBa_Q01122
location: https://synbiohub.org/public/igem/BBa_Q01122/annotation951728/range951728/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_Q01122/annotation951728/range951728/1
type: Range
persistent identity: range951728
display id: range951728
version: 1
top level: BBa_Q01122
direction: direction/forward
start: 1182
end: 1261
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation951754
display id: annotation951754
version: 1
top level: BBa_Q01122
location: https://synbiohub.org/public/igem/BBa_Q01122/annotation951754/range951754/1
component: BBa_R0010

https://synbiohub.org/public/igem/BBa_Q01122/annotation951754/range951754/1
type: Range
persistent identity: range951754
display id: range951754
version: 1
top level: BBa_Q01122
direction: direction/forward
start: 1319
end: 1518
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation951738
display id: annotation951738
version: 1
top level: BBa_Q01122
location: https://synbiohub.org/public/igem/BBa_Q01122/annotation951738/range951738/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_Q01122/annotation951738/range951738/1
type: Range
persistent identity: range951738
display id: range951738
version: 1
top level: BBa_Q01122
direction: direction/forward
start: 1270
end: 1310
orientation: inline

BBa_Q00121
type: ComponentDefinition
persistent identity: BBa Q00121
display id: BBa_Q00121
version: 1
was generated by: iGEM to SBOL conversion
description: LacI
created: 2004-07-19T11:00:00Z
modified: 2015-05-08T01:14:13Z
owned by: james
owned by: myers
mutable description: LacI QPI with B0030 which according to preliminary experiments is a "medium" strength RBS. Also uses hybrid promoter.
discontinued: false
dominant: false
experience: experience/None
group u list: _6_
m user id: 0
owner id: 101
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: mit
type: DnaRegion
role: SO:0000804
role: partType/Inverter
component: BBa_B0015
component: BBa_R0011
component: BBa_C0012
component: BBa_B0030
sequence annotation: BBa_B0030
sequence annotation: BBa_R0011
sequence annotation: BBa_C0012
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_Q00121_sequence/1

BBa_B0015
type: Component
persistent identity: component2221547
display id: component2221547
version: 1
top level: BBa_Q00121
access: public
definition: BBa_B0015

BBa_R0011
type: Component
persistent identity: component2221548
display id: component2221548
version: 1
top level: BBa_Q00121
access: public
definition: lacI+pL

BBa_C0012
type: Component
persistent identity: component2221538
display id: component2221538
version: 1
top level: BBa_Q00121
access: public
definition: lacI

BBa_B0030
type: Component
persistent identity: component2221535
display id: component2221535
version: 1
top level: BBa_Q00121
access: public
definition: BBa_B0030

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation2221535
display id: annotation2221535
version: 1
top level: BBa_Q00121
location: https://synbiohub.org/public/igem/BBa_Q00121/annotation2221535/range2221535/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_Q00121/annotation2221535/range2221535/1
type: Range
persistent identity: range2221535
display id: range2221535
version: 1
top level: BBa_Q00121
direction: direction/forward
start: 1
end: 15
orientation: inline

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation2221548
display id: annotation2221548
version: 1
top level: BBa_Q00121
location: https://synbiohub.org/public/igem/BBa_Q00121/annotation2221548/range2221548/1
component: BBa_R0011

https://synbiohub.org/public/igem/BBa_Q00121/annotation2221548/range2221548/1
type: Range
persistent identity: range2221548
display id: range2221548
version: 1
top level: BBa_Q00121
direction: direction/forward
start: 1320
end: 1373
orientation: inline

BBa_C0012
type: SequenceAnnotation
persistent identity: annotation2221538
display id: annotation2221538
version: 1
top level: BBa_Q00121
location: https://synbiohub.org/public/igem/BBa_Q00121/annotation2221538/range2221538/1
component: BBa_C0012

https://synbiohub.org/public/igem/BBa_Q00121/annotation2221538/range2221538/1
type: Range
persistent identity: range2221538
display id: range2221538
version: 1
top level: BBa_Q00121
direction: direction/forward
start: 22
end: 1149
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2221547
display id: annotation2221547
version: 1
top level: BBa_Q00121
location: https://synbiohub.org/public/igem/BBa_Q00121/annotation2221547/range2221547/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_Q00121/annotation2221547/range2221547/1
type: Range
persistent identity: range2221547
display id: range2221547
version: 1
top level: BBa_Q00121
direction: direction/reverse
start: 1183
end: 1311
orientation: inline

cI TP901
type: ComponentDefinition
persistent identity: BBa R0075
display id: BBa_R0075
version: 1
was generated by: iGEM to SBOL conversion
description: Promoter (TP901 cI regulated)
created: 2004-01-27T12:00:00Z
modified: 2015-05-08T01:14:15Z
mutable provenance: TP901-1
owned by: james
owned by: myers
mutable description: cI repressor from phage TP901 infecting L. lactis
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: operator sites: pR and pL
star: false
creator: crackdots
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: cI tp901 OL
sequence annotation: -10
sequence annotation: cI tp901 OR
sequence annotation: -35
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_R0075_sequence/1

cI tp901 OL
type: SequenceAnnotation
persistent identity: annotation319984
display id: annotation319984
version: 1
top level: cI TP901
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0075/annotation319984/range319984/1

https://synbiohub.org/public/igem/BBa_R0075/annotation319984/range319984/1
type: Range
persistent identity: range319984
display id: range319984
version: 1
top level: cI TP901
direction: direction/forward
start: 85
end: 115
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation319985
display id: annotation319985
version: 1
top level: cI TP901
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0075/annotation319985/range319985/1

https://synbiohub.org/public/igem/BBa_R0075/annotation319985/range319985/1
type: Range
persistent identity: range319985
display id: range319985
version: 1
top level: cI TP901
direction: direction/forward
start: 76
end: 82
orientation: inline

cI tp901 OR
type: SequenceAnnotation
persistent identity: annotation302714
display id: annotation302714
version: 1
top level: cI TP901
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0075/annotation302714/range302714/1

https://synbiohub.org/public/igem/BBa_R0075/annotation302714/range302714/1
type: Range
persistent identity: range302714
display id: range302714
version: 1
top level: cI TP901
direction: direction/forward
start: 19
end: 57
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation319983
display id: annotation319983
version: 1
top level: cI TP901
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0075/annotation319983/range319983/1

https://synbiohub.org/public/igem/BBa_R0075/annotation319983/range319983/1
type: Range
persistent identity: range319983
display id: range319983
version: 1
top level: cI TP901
direction: direction/forward
start: 53
end: 59
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation320591
display id: annotation320591
version: 1
top level: cI TP901
role: feature/start
role: SO:0000318
location: https://synbiohub.org/public/igem/BBa_R0075/annotation320591/range320591/1

https://synbiohub.org/public/igem/BBa_R0075/annotation320591/range320591/1
type: Range
persistent identity: range320591
display id: range320591
version: 1
top level: cI TP901
direction: direction/forward
start: 89
end: 89
orientation: inline

BBa_B0039
type: ComponentDefinition
persistent identity: BBa B0039
display id: BBa_B0039
version: 1
was generated by: iGEM to SBOL conversion
description: RBS 2
created: 2005-12-20T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Modified version of B0031 from the Voigt Lab. Not currently available as an independent part (only in a tet inverter).
discontinued: false
dominant: false
experience: experience/None
group u list: _11_
m user id: 0
owner id: 253
owning group id: 6
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Josh Michener
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_B0039_sequence/1

BBa_J06802
type: ComponentDefinition
persistent identity: BBa J06802
display id: BBa_J06802
version: 1
was generated by: iGEM to SBOL conversion
description: QPI (B0034.J06502.B0015.R0051)
created: 2005-06-13T11:00:00Z
modified: 2015-08-31T04:08:19Z
owned by: james
owned by: myers
mutable description: Lambda cI857 QPI w/ strong RBS
discontinued: true
dominant: false
experience: experience/None
group u list: _20_
m user id: 0
owner id: 340
owning group id: 20
sample status: Discontinued
status: status/Deleted
bookmark: false
mutable notes: First attempt to mutate failed. Suspect the problem is a low mutation rate due to results of positive control. May be a result of the mutagenesis kit being old. Second attempt to mutate failed. Abandoned,
star: false
creator: ytwang
type: DnaRegion
role: SO:0000804
role: partType/Inverter
component: BBa_J06502
component: BBa_B0010
component: BBa_R0051
component: BBa_B0034
component: BBa_B0012
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_R0051
sequence annotation: BBa_J06502
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_J06802_sequence/1

BBa_J06502
type: Component
persistent identity: component1552254
display id: component1552254
version: 1
top level: BBa_J06802
access: public
definition: cI ts

BBa_B0010
type: Component
persistent identity: component1552259
display id: component1552259
version: 1
top level: BBa_J06802
access: public
definition: BBa_B0010

BBa_R0051
type: Component
persistent identity: component1552284
display id: component1552284
version: 1
top level: BBa_J06802
access: public
definition: cI lam

BBa_B0034
type: Component
persistent identity: component1552234
display id: component1552234
version: 1
top level: BBa_J06802
access: public
definition: BBa_B0034

BBa_B0012
type: Component
persistent identity: component1552269
display id: component1552269
version: 1
top level: BBa_J06802
access: public
definition: BBa_B0012

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1552259
display id: annotation1552259
version: 1
top level: BBa_J06802
location: https://synbiohub.org/public/igem/BBa_J06802/annotation1552259/range1552259/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_J06802/annotation1552259/range1552259/1
type: Range
persistent identity: range1552259
display id: range1552259
version: 1
top level: BBa_J06802
direction: direction/forward
start: 802
end: 881
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1552269
display id: annotation1552269
version: 1
top level: BBa_J06802
location: https://synbiohub.org/public/igem/BBa_J06802/annotation1552269/range1552269/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_J06802/annotation1552269/range1552269/1
type: Range
persistent identity: range1552269
display id: range1552269
version: 1
top level: BBa_J06802
direction: direction/forward
start: 890
end: 930
orientation: inline

BBa_R0051
type: SequenceAnnotation
persistent identity: annotation1552284
display id: annotation1552284
version: 1
top level: BBa_J06802
location: https://synbiohub.org/public/igem/BBa_J06802/annotation1552284/range1552284/1
component: BBa_R0051

https://synbiohub.org/public/igem/BBa_J06802/annotation1552284/range1552284/1
type: Range
persistent identity: range1552284
display id: range1552284
version: 1
top level: BBa_J06802
direction: direction/forward
start: 939
end: 987
orientation: inline

BBa_J06502
type: SequenceAnnotation
persistent identity: annotation1552254
display id: annotation1552254
version: 1
top level: BBa_J06802
location: https://synbiohub.org/public/igem/BBa_J06802/annotation1552254/range1552254/1
component: BBa_J06502

https://synbiohub.org/public/igem/BBa_J06802/annotation1552254/range1552254/1
type: Range
persistent identity: range1552254
display id: range1552254
version: 1
top level: BBa_J06802
direction: direction/forward
start: 19
end: 793
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1552234
display id: annotation1552234
version: 1
top level: BBa_J06802
location: https://synbiohub.org/public/igem/BBa_J06802/annotation1552234/range1552234/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_J06802/annotation1552234/range1552234/1
type: Range
persistent identity: range1552234
display id: range1552234
version: 1
top level: BBa_J06802
direction: direction/forward
start: 1
end: 12
orientation: inline

cI
type: ComponentDefinition
persistent identity: BBa Q04511
display id: BBa_Q04511
version: 1
was generated by: iGEM to SBOL conversion
description: QPI (B0034.C0051.B0015.R0065)
created: 2003-12-02T12:00:00Z
modified: 2015-05-08T01:14:14Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Lambda cI QPI w/ strong RBS, hybrid promoter regulated by lambda cI and LuxR
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Caitlin Conboy and Jennifer Braff
type: DnaRegion
role: SO:0000804
role: partType/Inverter
component: BBa_B0012
component: BBa_B0034
component: BBa_R0065
component: BBa_B0010
component: BBa_C0051
sequence annotation: BBa_B0034
sequence annotation: BBa_R0065
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_C0051
sequence: https://synbiohub.org/public/igem/BBa_Q04511_sequence/1

BBa_B0012
type: Component
persistent identity: component952958
display id: component952958
version: 1
top level: cI
access: public
definition: BBa_B0012

BBa_B0034
type: Component
persistent identity: component952932
display id: component952932
version: 1
top level: cI
access: public
definition: BBa_B0034

BBa_R0065
type: Component
persistent identity: component952978
display id: component952978
version: 1
top level: cI
access: public
definition: cI+luxR

BBa_B0010
type: Component
persistent identity: component952948
display id: component952948
version: 1
top level: cI
access: public
definition: BBa_B0010

BBa_C0051
type: Component
persistent identity: component952942
display id: component952942
version: 1
top level: cI
access: public
definition: cI lam

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation952932
display id: annotation952932
version: 1
top level: cI
location: https://synbiohub.org/public/igem/BBa_Q04511/annotation952932/range952932/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_Q04511/annotation952932/range952932/1
type: Range
persistent identity: range952932
display id: range952932
version: 1
top level: cI
direction: direction/forward
start: 1
end: 12
orientation: inline

BBa_R0065
type: SequenceAnnotation
persistent identity: annotation952978
display id: annotation952978
version: 1
top level: cI
location: https://synbiohub.org/public/igem/BBa_Q04511/annotation952978/range952978/1
component: BBa_R0065

https://synbiohub.org/public/igem/BBa_Q04511/annotation952978/range952978/1
type: Range
persistent identity: range952978
display id: range952978
version: 1
top level: cI
direction: direction/forward
start: 939
end: 1035
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation952948
display id: annotation952948
version: 1
top level: cI
location: https://synbiohub.org/public/igem/BBa_Q04511/annotation952948/range952948/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_Q04511/annotation952948/range952948/1
type: Range
persistent identity: range952948
display id: range952948
version: 1
top level: cI
direction: direction/forward
start: 802
end: 881
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation952958
display id: annotation952958
version: 1
top level: cI
location: https://synbiohub.org/public/igem/BBa_Q04511/annotation952958/range952958/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_Q04511/annotation952958/range952958/1
type: Range
persistent identity: range952958
display id: range952958
version: 1
top level: cI
direction: direction/forward
start: 890
end: 930
orientation: inline

BBa_C0051
type: SequenceAnnotation
persistent identity: annotation952942
display id: annotation952942
version: 1
top level: cI
location: https://synbiohub.org/public/igem/BBa_Q04511/annotation952942/range952942/1
component: BBa_C0051

https://synbiohub.org/public/igem/BBa_Q04511/annotation952942/range952942/1
type: Range
persistent identity: range952942
display id: range952942
version: 1
top level: cI
direction: direction/forward
start: 19
end: 768
orientation: inline

PenI
type: ComponentDefinition
persistent identity: BBa R0074
display id: BBa_R0074
version: 1
was generated by: iGEM to SBOL conversion
description: Promoter (PenI regulated)
created: 2004-01-27T12:00:00Z
modified: 2015-05-08T01:14:15Z
mutable provenance: bacillus licheniformis
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: PenP operator from Bacillus Lichenformis. Two operator sites are negatively regulated by PenI (C0074).
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: extends slightly past -50
star: true
creator: crackdots
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: dimer left half
sequence annotation: PenI
sequence annotation: -10
sequence annotation: -35
sequence annotation: dimer right half
sequence: https://synbiohub.org/public/igem/BBa_R0074_sequence/1

dimer left half
type: SequenceAnnotation
persistent identity: annotation319788
display id: annotation319788
version: 1
top level: PenI
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0074/annotation319788/range319788/1

https://synbiohub.org/public/igem/BBa_R0074/annotation319788/range319788/1
type: Range
persistent identity: range319788
display id: range319788
version: 1
top level: PenI
direction: direction/forward
start: 31
end: 53
orientation: inline

PenI
type: SequenceAnnotation
persistent identity: annotation302713
display id: annotation302713
version: 1
top level: PenI
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_R0074/annotation302713/range302713/1

https://synbiohub.org/public/igem/BBa_R0074/annotation302713/range302713/1
type: Range
persistent identity: range302713
display id: range302713
version: 1
top level: PenI
direction: direction/forward
start: 1
end: 77
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation319212
display id: annotation319212
version: 1
top level: PenI
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0074/annotation319212/range319212/1

https://synbiohub.org/public/igem/BBa_R0074/annotation319212/range319212/1
type: Range
persistent identity: range319212
display id: range319212
version: 1
top level: PenI
direction: direction/forward
start: 46
end: 51
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation319211
display id: annotation319211
version: 1
top level: PenI
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0074/annotation319211/range319211/1

https://synbiohub.org/public/igem/BBa_R0074/annotation319211/range319211/1
type: Range
persistent identity: range319211
display id: range319211
version: 1
top level: PenI
direction: direction/forward
start: 23
end: 28
orientation: inline

dimer right half
type: SequenceAnnotation
persistent identity: annotation319789
display id: annotation319789
version: 1
top level: PenI
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0074/annotation319789/range319789/1

https://synbiohub.org/public/igem/BBa_R0074/annotation319789/range319789/1
type: Range
persistent identity: range319789
display id: range319789
version: 1
top level: PenI
direction: direction/forward
start: 55
end: 76
orientation: inline

BBa_I13409
type: ComponentDefinition
persistent identity: BBa I13409
display id: BBa_I13409
version: 1
was generated by: iGEM to SBOL conversion
description: Q04400 with additional REN sites
created: 2005-05-22T11:00:00Z
modified: 2015-08-31T04:07:34Z
owned by: james
owned by: myers
mutable description: HindIII - Q04400 - NcoI Note that this part does NOT have the standard biobrick ends and is available only as a PCR product.
discontinued: false
dominant: false
experience: experience/None
group u list: _11_
m user id: 0
owner id: 253
owning group id: 6
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: jkm
type: DnaRegion
role: SO:0000804
role: partType/Inverter
sequence: https://synbiohub.org/public/igem/BBa_I13409_sequence/1

BBa_I9003
type: ComponentDefinition
persistent identity: BBa I9003
display id: BBa_I9003
version: 1
was generated by: iGEM to SBOL conversion
description: QPI (tp901-cI) B0034.C0075.B0015.R0075
created: 2004-01-27T12:00:00Z
modified: 2015-08-31T04:08:11Z
owned by: james
owned by: myers
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: crackdots
type: DnaRegion
role: SO:0000804
role: partType/Inverter
component: BBa_B0012
component: BBa_R0075
component: BBa_B0010
component: BBa_B0034
component: BBa_C0075
sequence annotation: BBa_C0075
sequence annotation: BBa_R0075
sequence annotation: BBa_B0034
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_I9003_sequence/1

BBa_B0012
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persistent identity: component951234
display id: component951234
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top level: BBa_I9003
access: public
definition: BBa_B0012

BBa_R0075
type: Component
persistent identity: component951254
display id: component951254
version: 1
top level: BBa_I9003
access: public
definition: cI TP901

BBa_B0010
type: Component
persistent identity: component951224
display id: component951224
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top level: BBa_I9003
access: public
definition: BBa_B0010

BBa_B0034
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persistent identity: component951208
display id: component951208
version: 1
top level: BBa_I9003
access: public
definition: BBa_B0034

BBa_C0075
type: Component
persistent identity: component951218
display id: component951218
version: 1
top level: BBa_I9003
access: public
definition: cI TP901

BBa_C0075
type: SequenceAnnotation
persistent identity: annotation951218
display id: annotation951218
version: 1
top level: BBa_I9003
location: https://synbiohub.org/public/igem/BBa_I9003/annotation951218/range951218/1
component: BBa_C0075

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persistent identity: range951218
display id: range951218
version: 1
top level: BBa_I9003
direction: direction/forward
start: 19
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orientation: inline

BBa_R0075
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component: BBa_R0075

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persistent identity: range951254
display id: range951254
version: 1
top level: BBa_I9003
direction: direction/forward
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end: 884
orientation: inline

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display id: annotation951208
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location: https://synbiohub.org/public/igem/BBa_I9003/annotation951208/range951208/1
component: BBa_B0034

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persistent identity: range951208
display id: range951208
version: 1
top level: BBa_I9003
direction: direction/forward
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orientation: inline

BBa_B0010
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top level: BBa_I9003
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component: BBa_B0010

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persistent identity: range951224
display id: range951224
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persistent identity: range951234
display id: range951234
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end: 759
orientation: inline

BBa_Q04510
type: ComponentDefinition
persistent identity: BBa Q04510
display id: BBa_Q04510
version: 1
was generated by: iGEM to SBOL conversion
description: QPI (B0034.C0051.B0015.R0051)
created: 2003-12-02T12:00:00Z
modified: 2015-05-08T01:14:14Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Lambda cI QPI w/ strong RBS
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Caitlin Conboy and Jennifer Braff
type: DnaRegion
role: SO:0000804
role: partType/Inverter
component: BBa_C0051
component: BBa_B0034
component: BBa_B0010
component: BBa_B0012
component: BBa_R0051
sequence annotation: BBa_R0051
sequence annotation: BBa_B0012
sequence annotation: BBa_B0010
sequence annotation: BBa_C0051
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_Q04510_sequence/1

BBa_C0051
type: Component
persistent identity: component952895
display id: component952895
version: 1
top level: BBa_Q04510
access: public
definition: cI lam

BBa_B0034
type: Component
persistent identity: component952885
display id: component952885
version: 1
top level: BBa_Q04510
access: public
definition: BBa_B0034

BBa_B0010
type: Component
persistent identity: component952901
display id: component952901
version: 1
top level: BBa_Q04510
access: public
definition: BBa_B0010

BBa_B0012
type: Component
persistent identity: component952911
display id: component952911
version: 1
top level: BBa_Q04510
access: public
definition: BBa_B0012

BBa_R0051
type: Component
persistent identity: component952926
display id: component952926
version: 1
top level: BBa_Q04510
access: public
definition: cI lam

BBa_R0051
type: SequenceAnnotation
persistent identity: annotation952926
display id: annotation952926
version: 1
top level: BBa_Q04510
location: https://synbiohub.org/public/igem/BBa_Q04510/annotation952926/range952926/1
component: BBa_R0051

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persistent identity: range952926
display id: range952926
version: 1
top level: BBa_Q04510
direction: direction/forward
start: 939
end: 987
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation952911
display id: annotation952911
version: 1
top level: BBa_Q04510
location: https://synbiohub.org/public/igem/BBa_Q04510/annotation952911/range952911/1
component: BBa_B0012

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persistent identity: range952911
display id: range952911
version: 1
top level: BBa_Q04510
direction: direction/forward
start: 890
end: 930
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation952901
display id: annotation952901
version: 1
top level: BBa_Q04510
location: https://synbiohub.org/public/igem/BBa_Q04510/annotation952901/range952901/1
component: BBa_B0010

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persistent identity: range952901
display id: range952901
version: 1
top level: BBa_Q04510
direction: direction/forward
start: 802
end: 881
orientation: inline

BBa_C0051
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persistent identity: annotation952895
display id: annotation952895
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top level: BBa_Q04510
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component: BBa_C0051

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persistent identity: range952895
display id: range952895
version: 1
top level: BBa_Q04510
direction: direction/forward
start: 19
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orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation952885
display id: annotation952885
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top level: BBa_Q04510
location: https://synbiohub.org/public/igem/BBa_Q04510/annotation952885/range952885/1
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persistent identity: range952885
display id: range952885
version: 1
top level: BBa_Q04510
direction: direction/forward
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orientation: inline

BBa_J06803
type: ComponentDefinition
persistent identity: BBa J06803
display id: BBa_J06803
version: 1
was generated by: iGEM to SBOL conversion
description: QPI (B0034.J06503.B0015.R0051)
created: 2005-06-27T11:00:00Z
modified: 2015-08-31T04:08:19Z
owned by: james
owned by: myers
mutable description: Lambda cI857 QPI w/ strong RBS
discontinued: false
dominant: false
experience: experience/None
group u list: _20_
m user id: 0
owner id: 340
owning group id: 20
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: First attempt to mutate failed. Suspect the problem is a low mutation rate due to results of positive control. May be a result of the mutagenesis kit being old. Second attempt to mutate failed. Sent out to Blue Heron for synthesis.
star: false
creator: ytwang
type: DnaRegion
role: SO:0000804
role: partType/Inverter
component: BBa_B0012
component: BBa_R0051
component: BBa_J06503
component: BBa_B0010
component: BBa_B0034
sequence annotation: BBa_B0034
sequence annotation: BBa_B0012
sequence annotation: BBa_B0010
sequence annotation: BBa_R0051
sequence annotation: BBa_J06503
sequence: https://synbiohub.org/public/igem/BBa_J06803_sequence/1

BBa_B0012
type: Component
persistent identity: component1550025
display id: component1550025
version: 1
top level: BBa_J06803
access: public
definition: BBa_B0012

BBa_R0051
type: Component
persistent identity: component1550040
display id: component1550040
version: 1
top level: BBa_J06803
access: public
definition: cI lam

BBa_J06503
type: Component
persistent identity: component1550010
display id: component1550010
version: 1
top level: BBa_J06803
access: public
definition: cI857

BBa_B0010
type: Component
persistent identity: component1550015
display id: component1550015
version: 1
top level: BBa_J06803
access: public
definition: BBa_B0010

BBa_B0034
type: Component
persistent identity: component1549996
display id: component1549996
version: 1
top level: BBa_J06803
access: public
definition: BBa_B0034

BBa_B0034
type: SequenceAnnotation
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display id: annotation1549996
version: 1
top level: BBa_J06803
location: https://synbiohub.org/public/igem/BBa_J06803/annotation1549996/range1549996/1
component: BBa_B0034

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persistent identity: range1549996
display id: range1549996
version: 1
top level: BBa_J06803
direction: direction/forward
start: 1
end: 12
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1550025
display id: annotation1550025
version: 1
top level: BBa_J06803
location: https://synbiohub.org/public/igem/BBa_J06803/annotation1550025/range1550025/1
component: BBa_B0012

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type: Range
persistent identity: range1550025
display id: range1550025
version: 1
top level: BBa_J06803
direction: direction/forward
start: 890
end: 930
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1550015
display id: annotation1550015
version: 1
top level: BBa_J06803
location: https://synbiohub.org/public/igem/BBa_J06803/annotation1550015/range1550015/1
component: BBa_B0010

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type: Range
persistent identity: range1550015
display id: range1550015
version: 1
top level: BBa_J06803
direction: direction/forward
start: 802
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orientation: inline

BBa_R0051
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persistent identity: annotation1550040
display id: annotation1550040
version: 1
top level: BBa_J06803
location: https://synbiohub.org/public/igem/BBa_J06803/annotation1550040/range1550040/1
component: BBa_R0051

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type: Range
persistent identity: range1550040
display id: range1550040
version: 1
top level: BBa_J06803
direction: direction/forward
start: 939
end: 987
orientation: inline

BBa_J06503
type: SequenceAnnotation
persistent identity: annotation1550010
display id: annotation1550010
version: 1
top level: BBa_J06803
location: https://synbiohub.org/public/igem/BBa_J06803/annotation1550010/range1550010/1
component: BBa_J06503

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type: Range
persistent identity: range1550010
display id: range1550010
version: 1
top level: BBa_J06803
direction: direction/forward
start: 19
end: 793
orientation: inline

BBa_A340620
type: ComponentDefinition
persistent identity: BBa A340620
display id: BBa_A340620
version: 1
was generated by: iGEM to SBOL conversion
description: Quadpart Amplifier (B0034.C0062.B0015.R0062)
created: 2004-08-09T11:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: -- No description --
discontinued: false
dominant: false
experience: experience/None
group u list: _11_1_
m user id: 0
owner id: 60
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: false
creator: cconboy
type: DnaRegion
role: SO:0000804
role: partType/Inverter
component: BBa_B0034
component: BBa_B0012
component: BBa_R0062
component: BBa_C0062
component: BBa_B0010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0034
sequence annotation: BBa_C0062
sequence annotation: BBa_B0012
sequence annotation: BBa_R0062
sequence: https://synbiohub.org/public/igem/BBa_A340620_sequence/1

BBa_B0034
type: Component
persistent identity: component1041187
display id: component1041187
version: 1
top level: BBa_A340620
access: public
definition: BBa_B0034

BBa_B0012
type: Component
persistent identity: component1041220
display id: component1041220
version: 1
top level: BBa_A340620
access: public
definition: BBa_B0012

BBa_R0062
type: Component
persistent identity: component1041235
display id: component1041235
version: 1
top level: BBa_A340620
access: public
definition: lux pR

BBa_C0062
type: Component
persistent identity: component1041202
display id: component1041202
version: 1
top level: BBa_A340620
access: public
definition: luxr

BBa_B0010
type: Component
persistent identity: component1041210
display id: component1041210
version: 1
top level: BBa_A340620
access: public
definition: BBa_B0010

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1041210
display id: annotation1041210
version: 1
top level: BBa_A340620
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component: BBa_B0010

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persistent identity: range1041210
display id: range1041210
version: 1
top level: BBa_A340620
direction: direction/forward
start: 808
end: 887
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1041187
display id: annotation1041187
version: 1
top level: BBa_A340620
location: https://synbiohub.org/public/igem/BBa_A340620/annotation1041187/range1041187/1
component: BBa_B0034

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type: Range
persistent identity: range1041187
display id: range1041187
version: 1
top level: BBa_A340620
direction: direction/forward
start: 1
end: 12
orientation: inline

BBa_C0062
type: SequenceAnnotation
persistent identity: annotation1041202
display id: annotation1041202
version: 1
top level: BBa_A340620
location: https://synbiohub.org/public/igem/BBa_A340620/annotation1041202/range1041202/1
component: BBa_C0062

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type: Range
persistent identity: range1041202
display id: range1041202
version: 1
top level: BBa_A340620
direction: direction/forward
start: 19
end: 774
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1041220
display id: annotation1041220
version: 1
top level: BBa_A340620
location: https://synbiohub.org/public/igem/BBa_A340620/annotation1041220/range1041220/1
component: BBa_B0012

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type: Range
persistent identity: range1041220
display id: range1041220
version: 1
top level: BBa_A340620
direction: direction/forward
start: 896
end: 936
orientation: inline

BBa_R0062
type: SequenceAnnotation
persistent identity: annotation1041235
display id: annotation1041235
version: 1
top level: BBa_A340620
location: https://synbiohub.org/public/igem/BBa_A340620/annotation1041235/range1041235/1
component: BBa_R0062

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type: Range
persistent identity: range1041235
display id: range1041235
version: 1
top level: BBa_A340620
direction: direction/forward
start: 945
end: 999
orientation: inline

BBa_Q04122
type: ComponentDefinition
persistent identity: BBa Q04122
display id: BBa_Q04122
version: 1
was generated by: iGEM to SBOL conversion
description: QPI (B0034.C0012.B0015.R0010)
created: 2003-12-02T12:00:00Z
modified: 2015-05-08T01:14:13Z
owned by: james
owned by: myers
mutable description: LacI QPI with strong RBS
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Caitlin Conboy, Jennifer Braff
type: DnaRegion
role: SO:0000804
role: partType/Inverter
component: BBa_B0010
component: BBa_R0010
component: BBa_C0012
component: BBa_B0034
component: BBa_B0012
sequence annotation: BBa_B0010
sequence annotation: BBa_B0034
sequence annotation: BBa_R0010
sequence annotation: BBa_C0012
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_Q04122_sequence/1

BBa_B0010
type: Component
persistent identity: component952747
display id: component952747
version: 1
top level: BBa_Q04122
access: public
definition: BBa_B0010

BBa_R0010
type: Component
persistent identity: component952773
display id: component952773
version: 1
top level: BBa_Q04122
access: public
definition: LacI

BBa_C0012
type: Component
persistent identity: component952740
display id: component952740
version: 1
top level: BBa_Q04122
access: public
definition: lacI

BBa_B0034
type: Component
persistent identity: component952730
display id: component952730
version: 1
top level: BBa_Q04122
access: public
definition: BBa_B0034

BBa_B0012
type: Component
persistent identity: component952757
display id: component952757
version: 1
top level: BBa_Q04122
access: public
definition: BBa_B0012

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation952747
display id: annotation952747
version: 1
top level: BBa_Q04122
location: https://synbiohub.org/public/igem/BBa_Q04122/annotation952747/range952747/1
component: BBa_B0010

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type: Range
persistent identity: range952747
display id: range952747
version: 1
top level: BBa_Q04122
direction: direction/forward
start: 1180
end: 1259
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation952730
display id: annotation952730
version: 1
top level: BBa_Q04122
location: https://synbiohub.org/public/igem/BBa_Q04122/annotation952730/range952730/1
component: BBa_B0034

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type: Range
persistent identity: range952730
display id: range952730
version: 1
top level: BBa_Q04122
direction: direction/forward
start: 1
end: 12
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation952773
display id: annotation952773
version: 1
top level: BBa_Q04122
location: https://synbiohub.org/public/igem/BBa_Q04122/annotation952773/range952773/1
component: BBa_R0010

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type: Range
persistent identity: range952773
display id: range952773
version: 1
top level: BBa_Q04122
direction: direction/forward
start: 1317
end: 1516
orientation: inline

BBa_C0012
type: SequenceAnnotation
persistent identity: annotation952740
display id: annotation952740
version: 1
top level: BBa_Q04122
location: https://synbiohub.org/public/igem/BBa_Q04122/annotation952740/range952740/1
component: BBa_C0012

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type: Range
persistent identity: range952740
display id: range952740
version: 1
top level: BBa_Q04122
direction: direction/forward
start: 19
end: 1146
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation952757
display id: annotation952757
version: 1
top level: BBa_Q04122
location: https://synbiohub.org/public/igem/BBa_Q04122/annotation952757/range952757/1
component: BBa_B0012

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type: Range
persistent identity: range952757
display id: range952757
version: 1
top level: BBa_Q04122
direction: direction/forward
start: 1268
end: 1308
orientation: inline

lacI
type: ComponentDefinition
persistent identity: BBa C0012
display id: BBa_C0012
version: 1
was generated by: iGEM to SBOL conversion
description: lacI repressor from E. coli (+LVA)
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:23Z
mutable provenance: represillator of Elowitz and Leibler (2000)
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Coding region for the LacI protein with an LVA degradation tail and without an RBS. LacI binds to the pLac regulator <bb_part>BBa_R0010</bb_part> and PLlac01 hybrid regulator <bb_part>BBa_R0011</bb_part> and inhibits transcription. IPTG (Isopropylthiogalactoside) binds to LacI and inhibits its operation, therefore promoting transcription. A rapid degredation tail (LVA) has been added to improve the High to Low performance of this part.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: References (unparsed) here: Elowitz, M.B., Leibler, S. A synthetic oscillatory network of transcriptional regulators. Nature 403, 335-338 (2000). [ELOW00] References (unparsed) here: Elowitz, M.B., Leibler, S. A synthetic oscillatory network of transcriptional regulators. Nature 403, 335-338 (2000). [ELOW00] Sequence taken from the repressilator of Elowitz and Leibler (2000). The obtained sequence was compared to the wild-type sequence for LacI obtained through a database search. The sequence had been modified from the wild-type in that wild-type GTG start was changed to an ATG start (note, actual ORF in E.coli has several GTG starts it would seem). The LVA tag has been added for quicker degradation. Incompatible with systems containing LacI, lactose, or IPTG.
star: true
creator: Grace Kenney, Daniel Shen, Neelaksh Varshney, Samantha Sutton
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Help:Barcodes
sequence annotation: BBa_C0012
sequence annotation: lacI-LVA
sequence annotation: LVA
sequence: https://synbiohub.org/public/igem/BBa_C0012_sequence/1

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213988
display id: annotation2213988
version: 1
top level: lacI
role: SO:0000807
role: feature/barcode
location: https://synbiohub.org/public/igem/BBa_C0012/annotation2213988/range2213988/1

https://synbiohub.org/public/igem/BBa_C0012/annotation2213988/range2213988/1
type: Range
persistent identity: range2213988
display id: range2213988
version: 1
top level: lacI
direction: direction/forward
start: 1129
end: 1153
orientation: inline

BBa_C0012
type: SequenceAnnotation
persistent identity: annotation7031
display id: annotation7031
version: 1
top level: lacI
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_C0012/annotation7031/range7031/1

https://synbiohub.org/public/igem/BBa_C0012/annotation7031/range7031/1
type: Range
persistent identity: range7031
display id: range7031
version: 1
top level: lacI
direction: direction/forward
start: 1
end: 1128
orientation: inline

lacI-LVA
type: SequenceAnnotation
persistent identity: annotation1723
display id: annotation1723
version: 1
top level: lacI
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_C0012/annotation1723/range1723/1

https://synbiohub.org/public/igem/BBa_C0012/annotation1723/range1723/1
type: Range
persistent identity: range1723
display id: range1723
version: 1
top level: lacI
direction: direction/forward
start: 1
end: 1128
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation1722
display id: annotation1722
version: 1
top level: lacI
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_C0012/annotation1722/range1722/1

https://synbiohub.org/public/igem/BBa_C0012/annotation1722/range1722/1
type: Range
persistent identity: range1722
display id: range1722
version: 1
top level: lacI
direction: direction/forward
start: 1090
end: 1128
orientation: inline

BBa_J06801
type: ComponentDefinition
persistent identity: BBa J06801
display id: BBa_J06801
version: 1
was generated by: iGEM to SBOL conversion
description: QPI (B0034.J06501.B0015.R0011)
created: 2005-06-13T11:00:00Z
modified: 2015-08-31T04:08:19Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: LacI (ts) QPI w/ strong RBS
discontinued: false
dominant: false
experience: experience/None
group u list: _20_
m user id: 0
owner id: 340
owning group id: 20
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Mutation has been sequenced. The rest of the part will be sequenced shortly.
star: true
creator: ytwang
type: DnaRegion
role: SO:0000804
role: partType/Inverter
component: BBa_B0010
component: BBa_B0034
component: BBa_J06501
component: BBa_B0012
component: BBa_R0011
sequence annotation: BBa_R0011
sequence annotation: BBa_B0010
sequence annotation: BBa_B0034
sequence annotation: BBa_J06501
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_J06801_sequence/1

BBa_B0010
type: Component
persistent identity: component1531059
display id: component1531059
version: 1
top level: BBa_J06801
access: public
definition: BBa_B0010

BBa_B0034
type: Component
persistent identity: component1531040
display id: component1531040
version: 1
top level: BBa_J06801
access: public
definition: BBa_B0034

BBa_J06501
type: Component
persistent identity: component1531054
display id: component1531054
version: 1
top level: BBa_J06801
access: public
definition: lacI ts

BBa_B0012
type: Component
persistent identity: component1531069
display id: component1531069
version: 1
top level: BBa_J06801
access: public
definition: BBa_B0012

BBa_R0011
type: Component
persistent identity: component1531083
display id: component1531083
version: 1
top level: BBa_J06801
access: public
definition: lacI+pL

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation1531083
display id: annotation1531083
version: 1
top level: BBa_J06801
location: https://synbiohub.org/public/igem/BBa_J06801/annotation1531083/range1531083/1
component: BBa_R0011

https://synbiohub.org/public/igem/BBa_J06801/annotation1531083/range1531083/1
type: Range
persistent identity: range1531083
display id: range1531083
version: 1
top level: BBa_J06801
direction: direction/forward
start: 1317
end: 1370
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1531059
display id: annotation1531059
version: 1
top level: BBa_J06801
location: https://synbiohub.org/public/igem/BBa_J06801/annotation1531059/range1531059/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_J06801/annotation1531059/range1531059/1
type: Range
persistent identity: range1531059
display id: range1531059
version: 1
top level: BBa_J06801
direction: direction/forward
start: 1180
end: 1259
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1531040
display id: annotation1531040
version: 1
top level: BBa_J06801
location: https://synbiohub.org/public/igem/BBa_J06801/annotation1531040/range1531040/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_J06801/annotation1531040/range1531040/1
type: Range
persistent identity: range1531040
display id: range1531040
version: 1
top level: BBa_J06801
direction: direction/forward
start: 1
end: 12
orientation: inline

BBa_J06501
type: SequenceAnnotation
persistent identity: annotation1531054
display id: annotation1531054
version: 1
top level: BBa_J06801
location: https://synbiohub.org/public/igem/BBa_J06801/annotation1531054/range1531054/1
component: BBa_J06501

https://synbiohub.org/public/igem/BBa_J06801/annotation1531054/range1531054/1
type: Range
persistent identity: range1531054
display id: range1531054
version: 1
top level: BBa_J06801
direction: direction/forward
start: 19
end: 1171
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1531069
display id: annotation1531069
version: 1
top level: BBa_J06801
location: https://synbiohub.org/public/igem/BBa_J06801/annotation1531069/range1531069/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_J06801/annotation1531069/range1531069/1
type: Range
persistent identity: range1531069
display id: range1531069
version: 1
top level: BBa_J06801
direction: direction/forward
start: 1268
end: 1308
orientation: inline

cI+luxR
type: ComponentDefinition
persistent identity: BBa R0065
display id: BBa_R0065
version: 1
was generated by: iGEM to SBOL conversion
description: Promoter (lambda cI and luxR regulated -- hybrid)
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:15Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: cI repressor negatively regulates this promoter and LuxR activates its transcription.The effect of cI is dominant over LuxR. This part is based on the LuxR and cI repressor regulated hybrid promoter designed and tested by Ron Weiss. It requires the binding of two cI repressor dimers for maximal repression and contains two cI repressor binding sites namely, OR1 and OR2. This promoter is leaky in the sense that 'some' transcription is seen in the absence of both cI and LuxR. LuxI cI activity of promoter + + zero + - maximum - + zero - - leaky (no quantitative information)
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: This part was designed based on the LuxR and cI repressor regulated hybrid promoter tested by Ron Weiss and the LuxR-LuxICDABE sequence annotated by Tom Knight <genbank>AF170104</genbank>.
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -35
sequence annotation: OR2 cI
sequence annotation: lux p(R) start
sequence annotation: -10
sequence annotation: BBa_R0065
sequence annotation: Lux Box
sequence annotation: OR1 cI
sequence: https://synbiohub.org/public/igem/BBa_R0065_sequence/1

-35
type: SequenceAnnotation
persistent identity: annotation1986779
display id: annotation1986779
version: 1
top level: cI+luxR
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0065/annotation1986779/range1986779/1

https://synbiohub.org/public/igem/BBa_R0065/annotation1986779/range1986779/1
type: Range
persistent identity: range1986779
display id: range1986779
version: 1
top level: cI+luxR
direction: direction/forward
start: 71
end: 76
orientation: inline

OR2 cI
type: SequenceAnnotation
persistent identity: annotation1986777
display id: annotation1986777
version: 1
top level: cI+luxR
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0065/annotation1986777/range1986777/1

https://synbiohub.org/public/igem/BBa_R0065/annotation1986777/range1986777/1
type: Range
persistent identity: range1986777
display id: range1986777
version: 1
top level: cI+luxR
direction: direction/forward
start: 57
end: 73
orientation: inline

lux p(R) start
type: SequenceAnnotation
persistent identity: annotation1986778
display id: annotation1986778
version: 1
top level: cI+luxR
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0065/annotation1986778/range1986778/1

https://synbiohub.org/public/igem/BBa_R0065/annotation1986778/range1986778/1
type: Range
persistent identity: range1986778
display id: range1986778
version: 1
top level: cI+luxR
direction: direction/forward
start: 58
end: 58
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1986776
display id: annotation1986776
version: 1
top level: cI+luxR
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0065/annotation1986776/range1986776/1

https://synbiohub.org/public/igem/BBa_R0065/annotation1986776/range1986776/1
type: Range
persistent identity: range1986776
display id: range1986776
version: 1
top level: cI+luxR
direction: direction/forward
start: 47
end: 52
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1986781
display id: annotation1986781
version: 1
top level: cI+luxR
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0065/annotation1986781/range1986781/1

https://synbiohub.org/public/igem/BBa_R0065/annotation1986781/range1986781/1
type: Range
persistent identity: range1986781
display id: range1986781
version: 1
top level: cI+luxR
direction: direction/forward
start: 94
end: 97
orientation: inline

BBa_R0065
type: SequenceAnnotation
persistent identity: annotation1986774
display id: annotation1986774
version: 1
top level: cI+luxR
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_R0065/annotation1986774/range1986774/1

https://synbiohub.org/public/igem/BBa_R0065/annotation1986774/range1986774/1
type: Range
persistent identity: range1986774
display id: range1986774
version: 1
top level: cI+luxR
direction: direction/forward
start: 1
end: 97
orientation: inline

Lux Box
type: SequenceAnnotation
persistent identity: annotation1986775
display id: annotation1986775
version: 1
top level: cI+luxR
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0065/annotation1986775/range1986775/1

https://synbiohub.org/public/igem/BBa_R0065/annotation1986775/range1986775/1
type: Range
persistent identity: range1986775
display id: range1986775
version: 1
top level: cI+luxR
direction: direction/forward
start: 6
end: 25
orientation: inline

OR1 cI
type: SequenceAnnotation
persistent identity: annotation1986780
display id: annotation1986780
version: 1
top level: cI+luxR
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0065/annotation1986780/range1986780/1

https://synbiohub.org/public/igem/BBa_R0065/annotation1986780/range1986780/1
type: Range
persistent identity: range1986780
display id: range1986780
version: 1
top level: cI+luxR
direction: direction/forward
start: 81
end: 97
orientation: inline

tetR
type: ComponentDefinition
persistent identity: BBa Q04400
display id: BBa_Q04400
version: 1
was generated by: iGEM to SBOL conversion
description: QPI (B0034.C0040.B0015.R0040)
created: 2003-12-02T12:00:00Z
modified: 2015-05-08T01:14:13Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: TetR QPI with strong RBS
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Caitlin Conboy and Jennifer Braff
type: DnaRegion
role: SO:0000804
role: partType/Inverter
component: BBa_C0040
component: BBa_B0034
component: BBa_R0040
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_B0034
sequence annotation: BBa_B0010
sequence annotation: BBa_R0040
sequence annotation: BBa_C0040
sequence: https://synbiohub.org/public/igem/BBa_Q04400_sequence/1

BBa_C0040
type: Component
persistent identity: component952791
display id: component952791
version: 1
top level: tetR
access: public
definition: tetR

BBa_B0034
type: Component
persistent identity: component952781
display id: component952781
version: 1
top level: tetR
access: public
definition: BBa_B0034

BBa_R0040
type: Component
persistent identity: component952822
display id: component952822
version: 1
top level: tetR
access: public
definition: p(tetR)

BBa_B0012
type: Component
persistent identity: component952807
display id: component952807
version: 1
top level: tetR
access: public
definition: BBa_B0012

BBa_B0010
type: Component
persistent identity: component952797
display id: component952797
version: 1
top level: tetR
access: public
definition: BBa_B0010

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation952807
display id: annotation952807
version: 1
top level: tetR
location: https://synbiohub.org/public/igem/BBa_Q04400/annotation952807/range952807/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_Q04400/annotation952807/range952807/1
type: Range
persistent identity: range952807
display id: range952807
version: 1
top level: tetR
direction: direction/forward
start: 800
end: 840
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation952781
display id: annotation952781
version: 1
top level: tetR
location: https://synbiohub.org/public/igem/BBa_Q04400/annotation952781/range952781/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_Q04400/annotation952781/range952781/1
type: Range
persistent identity: range952781
display id: range952781
version: 1
top level: tetR
direction: direction/forward
start: 1
end: 12
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation952797
display id: annotation952797
version: 1
top level: tetR
location: https://synbiohub.org/public/igem/BBa_Q04400/annotation952797/range952797/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_Q04400/annotation952797/range952797/1
type: Range
persistent identity: range952797
display id: range952797
version: 1
top level: tetR
direction: direction/forward
start: 712
end: 791
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation952822
display id: annotation952822
version: 1
top level: tetR
location: https://synbiohub.org/public/igem/BBa_Q04400/annotation952822/range952822/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_Q04400/annotation952822/range952822/1
type: Range
persistent identity: range952822
display id: range952822
version: 1
top level: tetR
direction: direction/forward
start: 849
end: 902
orientation: inline

BBa_C0040
type: SequenceAnnotation
persistent identity: annotation952791
display id: annotation952791
version: 1
top level: tetR
location: https://synbiohub.org/public/igem/BBa_Q04400/annotation952791/range952791/1
component: BBa_C0040

https://synbiohub.org/public/igem/BBa_Q04400/annotation952791/range952791/1
type: Range
persistent identity: range952791
display id: range952791
version: 1
top level: tetR
direction: direction/forward
start: 19
end: 678
orientation: inline

BBa_B0032
type: ComponentDefinition
persistent identity: BBa B0032
display id: BBa_B0032
version: 1
was generated by: iGEM to SBOL conversion
description: RBS.3 (medium) -- derivative of BBa_0030
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Weak1 RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0033</bb_part>.
discontinued: false
dominant: true
experience: experience/Works
group u list: _41_44_48_46_1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("RBS-2" in figure 4-14 of thesis). Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: BBa_B0032
sequence annotation: RBS-3\Weak
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_B0032_sequence/1

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation7027
display id: annotation7027
version: 1
top level: BBa_B0032
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_B0032/annotation7027/range7027/1

https://synbiohub.org/public/igem/BBa_B0032/annotation7027/range7027/1
type: Range
persistent identity: range7027
display id: range7027
version: 1
top level: BBa_B0032
direction: direction/forward
start: 1
end: 13
orientation: inline

RBS-3\Weak
type: SequenceAnnotation
persistent identity: annotation1709
display id: annotation1709
version: 1
top level: BBa_B0032
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_B0032/annotation1709/range1709/1

https://synbiohub.org/public/igem/BBa_B0032/annotation1709/range1709/1
type: Range
persistent identity: range1709
display id: range1709
version: 1
top level: BBa_B0032
direction: direction/forward
start: 1
end: 13
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation1710
display id: annotation1710
version: 1
top level: BBa_B0032
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_B0032/annotation1710/range1710/1

https://synbiohub.org/public/igem/BBa_B0032/annotation1710/range1710/1
type: Range
persistent identity: range1710
display id: range1710
version: 1
top level: BBa_B0032
direction: direction/forward
start: 7
end: 10
orientation: inline

BBa_Q02121
type: ComponentDefinition
persistent identity: BBa Q02121
display id: BBa_Q02121
version: 1
was generated by: iGEM to SBOL conversion
description: LacI
created: 2004-07-19T11:00:00Z
modified: 2015-05-08T01:14:13Z
owned by: james
owned by: myers
mutable description: LacI QPI with B0032 which according to preliminary experiments is a "medium" strength RBS. Also uses hybrid promoter.
discontinued: false
dominant: false
experience: experience/None
group u list: _6_
m user id: 0
owner id: 101
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: mit
type: DnaRegion
role: SO:0000804
role: partType/Inverter
component: BBa_B0015
component: BBa_R0011
component: BBa_B0032
component: BBa_C0012
sequence annotation: BBa_C0012
sequence annotation: BBa_B0015
sequence annotation: BBa_B0032
sequence annotation: BBa_R0011
sequence: https://synbiohub.org/public/igem/BBa_Q02121_sequence/1

BBa_B0015
type: Component
persistent identity: component2221528
display id: component2221528
version: 1
top level: BBa_Q02121
access: public
definition: BBa_B0015

BBa_R0011
type: Component
persistent identity: component2221529
display id: component2221529
version: 1
top level: BBa_Q02121
access: public
definition: lacI+pL

BBa_B0032
type: Component
persistent identity: component2221516
display id: component2221516
version: 1
top level: BBa_Q02121
access: public
definition: BBa_B0032

BBa_C0012
type: Component
persistent identity: component2221519
display id: component2221519
version: 1
top level: BBa_Q02121
access: public
definition: lacI

BBa_C0012
type: SequenceAnnotation
persistent identity: annotation2221519
display id: annotation2221519
version: 1
top level: BBa_Q02121
location: https://synbiohub.org/public/igem/BBa_Q02121/annotation2221519/range2221519/1
component: BBa_C0012

https://synbiohub.org/public/igem/BBa_Q02121/annotation2221519/range2221519/1
type: Range
persistent identity: range2221519
display id: range2221519
version: 1
top level: BBa_Q02121
direction: direction/forward
start: 20
end: 1147
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2221528
display id: annotation2221528
version: 1
top level: BBa_Q02121
location: https://synbiohub.org/public/igem/BBa_Q02121/annotation2221528/range2221528/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_Q02121/annotation2221528/range2221528/1
type: Range
persistent identity: range2221528
display id: range2221528
version: 1
top level: BBa_Q02121
direction: direction/reverse
start: 1181
end: 1309
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation2221516
display id: annotation2221516
version: 1
top level: BBa_Q02121
location: https://synbiohub.org/public/igem/BBa_Q02121/annotation2221516/range2221516/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_Q02121/annotation2221516/range2221516/1
type: Range
persistent identity: range2221516
display id: range2221516
version: 1
top level: BBa_Q02121
direction: direction/forward
start: 1
end: 13
orientation: inline

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation2221529
display id: annotation2221529
version: 1
top level: BBa_Q02121
location: https://synbiohub.org/public/igem/BBa_Q02121/annotation2221529/range2221529/1
component: BBa_R0011

https://synbiohub.org/public/igem/BBa_Q02121/annotation2221529/range2221529/1
type: Range
persistent identity: range2221529
display id: range2221529
version: 1
top level: BBa_Q02121
direction: direction/forward
start: 1318
end: 1371
orientation: inline

BBa_B0015
type: ComponentDefinition
persistent identity: BBa B0015
display id: BBa_B0015
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0010-B0012)
created: 2003-07-16T11:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Double terminator consisting of BBa_B0010 and BBa_B0012
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_B0010
sequence: https://synbiohub.org/public/igem/BBa_B0015_sequence/1

BBa_B0012
type: Component
persistent identity: component1916612
display id: component1916612
version: 1
top level: BBa_B0015
access: public
definition: BBa_B0012

BBa_B0010
type: Component
persistent identity: component1916610
display id: component1916610
version: 1
top level: BBa_B0015
access: public
definition: BBa_B0010

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1916612
display id: annotation1916612
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
type: Range
persistent identity: range1916612
display id: range1916612
version: 1
top level: BBa_B0015
direction: direction/forward
start: 89
end: 129
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1916610
display id: annotation1916610
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
type: Range
persistent identity: range1916610
display id: range1916610
version: 1
top level: BBa_B0015
direction: direction/forward
start: 1
end: 80
orientation: inline

BBa_Q08400
type: ComponentDefinition
persistent identity: BBa Q08400
display id: BBa_Q08400
version: 1
was generated by: iGEM to SBOL conversion
description: tetR inverter, RBS1
created: 2005-12-20T12:00:00Z
modified: 2015-05-08T01:14:14Z
owned by: james
owned by: myers
mutable description: Inverter from the Voigt Lab
discontinued: false
dominant: false
group u list: _11_
m user id: 0
owner id: 253
owning group id: 6
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Josh Michener
type: DnaRegion
role: SO:0000804
role: partType/Inverter
component: BBa_R0040
component: BBa_B0038
component: BBa_B0012
component: BBa_B0010
component: BBa_C0040
sequence annotation: BBa_B0010
sequence annotation: BBa_C0040
sequence annotation: BBa_B0012
sequence annotation: BBa_R0040
sequence annotation: BBa_B0038
sequence: https://synbiohub.org/public/igem/BBa_Q08400_sequence/1

BBa_R0040
type: Component
persistent identity: component1771826
display id: component1771826
version: 1
top level: BBa_Q08400
access: public
definition: p(tetR)

BBa_B0038
type: Component
persistent identity: component1771785
display id: component1771785
version: 1
top level: BBa_Q08400
access: public
definition: BBa_B0038

BBa_B0012
type: Component
persistent identity: component1771811
display id: component1771811
version: 1
top level: BBa_Q08400
access: public
definition: BBa_B0012

BBa_B0010
type: Component
persistent identity: component1771801
display id: component1771801
version: 1
top level: BBa_Q08400
access: public
definition: BBa_B0010

BBa_C0040
type: Component
persistent identity: component1771795
display id: component1771795
version: 1
top level: BBa_Q08400
access: public
definition: tetR

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1771801
display id: annotation1771801
version: 1
top level: BBa_Q08400
location: https://synbiohub.org/public/igem/BBa_Q08400/annotation1771801/range1771801/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_Q08400/annotation1771801/range1771801/1
type: Range
persistent identity: range1771801
display id: range1771801
version: 1
top level: BBa_Q08400
direction: direction/forward
start: 723
end: 802
orientation: inline

BBa_C0040
type: SequenceAnnotation
persistent identity: annotation1771795
display id: annotation1771795
version: 1
top level: BBa_Q08400
location: https://synbiohub.org/public/igem/BBa_Q08400/annotation1771795/range1771795/1
component: BBa_C0040

https://synbiohub.org/public/igem/BBa_Q08400/annotation1771795/range1771795/1
type: Range
persistent identity: range1771795
display id: range1771795
version: 1
top level: BBa_Q08400
direction: direction/forward
start: 30
end: 689
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1771811
display id: annotation1771811
version: 1
top level: BBa_Q08400
location: https://synbiohub.org/public/igem/BBa_Q08400/annotation1771811/range1771811/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_Q08400/annotation1771811/range1771811/1
type: Range
persistent identity: range1771811
display id: range1771811
version: 1
top level: BBa_Q08400
direction: direction/forward
start: 811
end: 851
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1771826
display id: annotation1771826
version: 1
top level: BBa_Q08400
location: https://synbiohub.org/public/igem/BBa_Q08400/annotation1771826/range1771826/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_Q08400/annotation1771826/range1771826/1
type: Range
persistent identity: range1771826
display id: range1771826
version: 1
top level: BBa_Q08400
direction: direction/forward
start: 860
end: 913
orientation: inline

BBa_B0038
type: SequenceAnnotation
persistent identity: annotation1771785
display id: annotation1771785
version: 1
top level: BBa_Q08400
location: https://synbiohub.org/public/igem/BBa_Q08400/annotation1771785/range1771785/1
component: BBa_B0038

https://synbiohub.org/public/igem/BBa_Q08400/annotation1771785/range1771785/1
type: Range
persistent identity: range1771785
display id: range1771785
version: 1
top level: BBa_Q08400
direction: direction/forward
start: 1
end: 23
orientation: inline

cI ts
type: ComponentDefinition
persistent identity: BBa J06502
display id: BBa_J06502
version: 1
was generated by: iGEM to SBOL conversion
description: Lambda cI857 (E07700) (RBS- LVA+)
created: 2005-06-13T11:00:00Z
modified: 2015-08-31T04:08:18Z
mutable provenance: GenBank E00770 (Japanese Patent)
owned by: james
owned by: myers
mutable description: Mutated from BBa_C0051 Repressor, Lambda cI (RBS- LVA+) to be temperature sensitive and UV insensitive.
discontinued: true
dominant: false
experience: experience/None
group u list: _20_
m user id: 0
owner id: 340
owning group id: 20
sample status: Discontinued
status: status/Deleted
bookmark: false
mutable notes: First attempt to mutate failed. Suspect the problem is a low mutation rate due to results of positive control. May be a result of the mutagenesis kit being old. Second attempt to mutation failed. The best we got was 2 out of 3 mutations. The sequencing had a lot of bad sequence at the beginings, so that the ind- mutation was not sequenced by either of the primers used. The ind- mutation was tested by cutting with HindIII. This part is being put on hold. We may finish it by doing a single site mutation on one of the mutants with the other 2 sites successfully mutated.
star: false
creator: ytwang
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: C->G
sequence annotation: G->C
sequence annotation: G->A (ind-)
sequence annotation: cI857
sequence annotation: LVA
sequence annotation: barcode from lambda cI (BBa_C0051)
sequence: https://synbiohub.org/public/igem/BBa_J06502_sequence/1

C->G
type: SequenceAnnotation
persistent identity: annotation1550572
display id: annotation1550572
version: 1
top level: cI ts
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_J06502/annotation1550572/range1550572/1

https://synbiohub.org/public/igem/BBa_J06502/annotation1550572/range1550572/1
type: Range
persistent identity: range1550572
display id: range1550572
version: 1
top level: cI ts
direction: direction/forward
start: 681
end: 681
orientation: inline

G->C
type: SequenceAnnotation
persistent identity: annotation1550568
display id: annotation1550568
version: 1
top level: cI ts
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_J06502/annotation1550568/range1550568/1

https://synbiohub.org/public/igem/BBa_J06502/annotation1550568/range1550568/1
type: Range
persistent identity: range1550568
display id: range1550568
version: 1
top level: cI ts
direction: direction/forward
start: 248
end: 248
orientation: inline

G->A (ind-)
type: SequenceAnnotation
persistent identity: annotation1550569
display id: annotation1550569
version: 1
top level: cI ts
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_J06502/annotation1550569/range1550569/1

https://synbiohub.org/public/igem/BBa_J06502/annotation1550569/range1550569/1
type: Range
persistent identity: range1550569
display id: range1550569
version: 1
top level: cI ts
direction: direction/forward
start: 352
end: 352
orientation: inline

cI857
type: SequenceAnnotation
persistent identity: annotation1531454
display id: annotation1531454
version: 1
top level: cI ts
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J06502/annotation1531454/range1531454/1

https://synbiohub.org/public/igem/BBa_J06502/annotation1531454/range1531454/1
type: Range
persistent identity: range1531454
display id: range1531454
version: 1
top level: cI ts
direction: direction/forward
start: 1
end: 711
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation1531464
display id: annotation1531464
version: 1
top level: cI ts
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_J06502/annotation1531464/range1531464/1

https://synbiohub.org/public/igem/BBa_J06502/annotation1531464/range1531464/1
type: Range
persistent identity: range1531464
display id: range1531464
version: 1
top level: cI ts
direction: direction/forward
start: 712
end: 744
orientation: inline

barcode from lambda cI (BBa_C0051)
type: SequenceAnnotation
persistent identity: annotation1552045
display id: annotation1552045
version: 1
top level: cI ts
role: SO:0000807
role: feature/barcode
location: https://synbiohub.org/public/igem/BBa_J06502/annotation1552045/range1552045/1

https://synbiohub.org/public/igem/BBa_J06502/annotation1552045/range1552045/1
type: Range
persistent identity: range1552045
display id: range1552045
version: 1
top level: cI ts
direction: direction/forward
start: 751
end: 775
orientation: inline

cI 434
type: ComponentDefinition
persistent identity: BBa R0052
display id: BBa_R0052
version: 1
was generated by: iGEM to SBOL conversion
description: Promoter (434 cI regulated)
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: Bacteriophage 434 right operator
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: The 434 cI regulatory region sequence is a 89 base-pair sequence with the standard BioBrick prefix and suffix sections on its ends. 434 cI repressor protein, <bb_part>BBa_C0052<bb_part>, binds to it. This segment contains O-R1, O-R2, P-R, and P-RM -35.
discontinued: false
dominant: false
experience: experience/Issues
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Maia Mahoney
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -35
sequence annotation: -10
sequence annotation: OR2
sequence annotation: OR1
sequence annotation: BBa_R0052
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_R0052_sequence/1

-35
type: SequenceAnnotation
persistent identity: annotation2031
display id: annotation2031
version: 1
top level: cI 434
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0052/annotation2031/range2031/1

https://synbiohub.org/public/igem/BBa_R0052/annotation2031/range2031/1
type: Range
persistent identity: range2031
display id: range2031
version: 1
top level: cI 434
direction: direction/forward
start: 2
end: 7
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2030
display id: annotation2030
version: 1
top level: cI 434
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0052/annotation2030/range2030/1

https://synbiohub.org/public/igem/BBa_R0052/annotation2030/range2030/1
type: Range
persistent identity: range2030
display id: range2030
version: 1
top level: cI 434
direction: direction/forward
start: 24
end: 29
orientation: inline

OR2
type: SequenceAnnotation
persistent identity: annotation2027
display id: annotation2027
version: 1
top level: cI 434
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0052/annotation2027/range2027/1

https://synbiohub.org/public/igem/BBa_R0052/annotation2027/range2027/1
type: Range
persistent identity: range2027
display id: range2027
version: 1
top level: cI 434
direction: direction/forward
start: 8
end: 21
orientation: inline

OR1
type: SequenceAnnotation
persistent identity: annotation2028
display id: annotation2028
version: 1
top level: cI 434
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0052/annotation2028/range2028/1

https://synbiohub.org/public/igem/BBa_R0052/annotation2028/range2028/1
type: Range
persistent identity: range2028
display id: range2028
version: 1
top level: cI 434
direction: direction/forward
start: 30
end: 43
orientation: inline

OR2
type: SequenceAnnotation
persistent identity: annotation2026
display id: annotation2026
version: 1
top level: cI 434
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0052/annotation2026/range2026/1

https://synbiohub.org/public/igem/BBa_R0052/annotation2026/range2026/1
type: Range
persistent identity: range2026
display id: range2026
version: 1
top level: cI 434
direction: direction/forward
start: 1
end: 43
orientation: inline

BBa_R0052
type: SequenceAnnotation
persistent identity: annotation7068
display id: annotation7068
version: 1
top level: cI 434
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_R0052/annotation7068/range7068/1

https://synbiohub.org/public/igem/BBa_R0052/annotation7068/range7068/1
type: Range
persistent identity: range7068
display id: range7068
version: 1
top level: cI 434
direction: direction/forward
start: 1
end: 46
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2032
display id: annotation2032
version: 1
top level: cI 434
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_R0052/annotation2032/range2032/1

https://synbiohub.org/public/igem/BBa_R0052/annotation2032/range2032/1
type: Range
persistent identity: range2032
display id: range2032
version: 1
top level: cI 434
direction: direction/forward
start: 35
end: 37
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2029
display id: annotation2029
version: 1
top level: cI 434
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0052/annotation2029/range2029/1

https://synbiohub.org/public/igem/BBa_R0052/annotation2029/range2029/1
type: Range
persistent identity: range2029
display id: range2029
version: 1
top level: cI 434
direction: direction/forward
start: 1
end: 6
orientation: inline

BBa_Q04520
type: ComponentDefinition
persistent identity: BBa Q04520
display id: BBa_Q04520
version: 1
was generated by: iGEM to SBOL conversion
description: QPI (B0034.C0052.B0015.R0052)
created: 2003-12-02T12:00:00Z
modified: 2015-05-08T01:14:14Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: 434 cI QPI w/ strong RBS
discontinued: false
dominant: false
experience: experience/Issues
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: false
creator: Caitlin Conboy and Jennifer Braff
type: DnaRegion
role: SO:0000804
role: partType/Inverter
component: BBa_B0034
component: BBa_C0052
component: BBa_B0010
component: BBa_B0012
component: BBa_R0052
sequence annotation: BBa_C0052
sequence annotation: BBa_B0034
sequence annotation: BBa_B0012
sequence annotation: BBa_B0010
sequence annotation: BBa_R0052
sequence: https://synbiohub.org/public/igem/BBa_Q04520_sequence/1

BBa_B0034
type: Component
persistent identity: component952988
display id: component952988
version: 1
top level: BBa_Q04520
access: public
definition: BBa_B0034

BBa_C0052
type: Component
persistent identity: component952998
display id: component952998
version: 1
top level: BBa_Q04520
access: public
definition: cI 434

BBa_B0010
type: Component
persistent identity: component953005
display id: component953005
version: 1
top level: BBa_Q04520
access: public
definition: BBa_B0010

BBa_B0012
type: Component
persistent identity: component953015
display id: component953015
version: 1
top level: BBa_Q04520
access: public
definition: BBa_B0012

BBa_R0052
type: Component
persistent identity: component953037
display id: component953037
version: 1
top level: BBa_Q04520
access: public
definition: cI 434

BBa_C0052
type: SequenceAnnotation
persistent identity: annotation952998
display id: annotation952998
version: 1
top level: BBa_Q04520
location: https://synbiohub.org/public/igem/BBa_Q04520/annotation952998/range952998/1
component: BBa_C0052

https://synbiohub.org/public/igem/BBa_Q04520/annotation952998/range952998/1
type: Range
persistent identity: range952998
display id: range952998
version: 1
top level: BBa_Q04520
direction: direction/forward
start: 19
end: 687
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation952988
display id: annotation952988
version: 1
top level: BBa_Q04520
location: https://synbiohub.org/public/igem/BBa_Q04520/annotation952988/range952988/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_Q04520/annotation952988/range952988/1
type: Range
persistent identity: range952988
display id: range952988
version: 1
top level: BBa_Q04520
direction: direction/forward
start: 1
end: 12
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation953015
display id: annotation953015
version: 1
top level: BBa_Q04520
location: https://synbiohub.org/public/igem/BBa_Q04520/annotation953015/range953015/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_Q04520/annotation953015/range953015/1
type: Range
persistent identity: range953015
display id: range953015
version: 1
top level: BBa_Q04520
direction: direction/forward
start: 809
end: 849
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation953005
display id: annotation953005
version: 1
top level: BBa_Q04520
location: https://synbiohub.org/public/igem/BBa_Q04520/annotation953005/range953005/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_Q04520/annotation953005/range953005/1
type: Range
persistent identity: range953005
display id: range953005
version: 1
top level: BBa_Q04520
direction: direction/forward
start: 721
end: 800
orientation: inline

BBa_R0052
type: SequenceAnnotation
persistent identity: annotation953037
display id: annotation953037
version: 1
top level: BBa_Q04520
location: https://synbiohub.org/public/igem/BBa_Q04520/annotation953037/range953037/1
component: BBa_R0052

https://synbiohub.org/public/igem/BBa_Q04520/annotation953037/range953037/1
type: Range
persistent identity: range953037
display id: range953037
version: 1
top level: BBa_Q04520
direction: direction/forward
start: 858
end: 903
orientation: inline

cI 434
type: ComponentDefinition
persistent identity: BBa C0052
display id: BBa_C0052
version: 1
was generated by: iGEM to SBOL conversion
description: cI repressor from phage 434 (+LVA)
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:23Z
mutable provenance: Bacteriophage 434
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: The 434 cI repressor protein coding sequence is a 710 base-pair sequence with the standard RBS-compatible BioBrick prefix and the standard BioBrick suffix sections on its ends. It binds to the 434 regulatory sequence, BBa_R0052. The sequence contains a LVA tag for faster degredation and has no RBS.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: References (unparsed) here: Nikolnikov,S., Posfai,G. and Sain,B. The construction of a versatile plasmid vector that allows direct selection of fragments cloned into six unique sites of the cI gene of coliphage 434. Gene 30 (1-3), 261-265 (1984) References (unparsed) here: Nikolnikov,S., Posfai,G. and Sain,B. The construction of a versatile plasmid vector that allows direct selection of fragments cloned into six unique sites of the cI gene of coliphage 434. Gene 30 (1-3), 261-265 (1984)
star: true
creator: Maia Mahoney
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: BBa_C0052
sequence annotation: cI 434
sequence annotation: Help:Barcodes
sequence annotation: LVA
sequence: https://synbiohub.org/public/igem/BBa_C0052_sequence/1

BBa_C0052
type: SequenceAnnotation
persistent identity: annotation7035
display id: annotation7035
version: 1
top level: cI 434
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_C0052/annotation7035/range7035/1

https://synbiohub.org/public/igem/BBa_C0052/annotation7035/range7035/1
type: Range
persistent identity: range7035
display id: range7035
version: 1
top level: cI 434
direction: direction/forward
start: 1
end: 669
orientation: inline

cI 434
type: SequenceAnnotation
persistent identity: annotation1743
display id: annotation1743
version: 1
top level: cI 434
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_C0052/annotation1743/range1743/1

https://synbiohub.org/public/igem/BBa_C0052/annotation1743/range1743/1
type: Range
persistent identity: range1743
display id: range1743
version: 1
top level: cI 434
direction: direction/forward
start: 1
end: 669
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213992
display id: annotation2213992
version: 1
top level: cI 434
role: SO:0000807
role: feature/barcode
location: https://synbiohub.org/public/igem/BBa_C0052/annotation2213992/range2213992/1

https://synbiohub.org/public/igem/BBa_C0052/annotation2213992/range2213992/1
type: Range
persistent identity: range2213992
display id: range2213992
version: 1
top level: cI 434
direction: direction/forward
start: 670
end: 694
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation1745
display id: annotation1745
version: 1
top level: cI 434
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_C0052/annotation1745/range1745/1

https://synbiohub.org/public/igem/BBa_C0052/annotation1745/range1745/1
type: Range
persistent identity: range1745
display id: range1745
version: 1
top level: cI 434
direction: direction/forward
start: 631
end: 669
orientation: inline

BBa_B0031
type: ComponentDefinition
persistent identity: BBa B0031
display id: BBa_B0031
version: 1
was generated by: iGEM to SBOL conversion
description: RBS.2 (weak) -- derivative of BBa_0030
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Medium RBS based on Ron Weiss thesis. Strength considered relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>.
discontinued: false
dominant: true
experience: experience/Works
group u list: _41_44_48_46_1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("RBS-1" in figure 4-14 of thesis). No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Cho
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0031_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23316
display id: annotation23316
version: 1
top level: BBa_B0031
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_B0031/annotation23316/range23316/1

https://synbiohub.org/public/igem/BBa_B0031/annotation23316/range23316/1
type: Range
persistent identity: range23316
display id: range23316
version: 1
top level: BBa_B0031
direction: direction/forward
start: 7
end: 10
orientation: inline

p(tetR)
type: ComponentDefinition
persistent identity: BBa R0040
display id: BBa_R0040
version: 1
was generated by: iGEM to SBOL conversion
description: TetR repressible promoter
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: Lutz, R., Bujard, H., Nucleic Acids Research (1997) 25, 1203-1210.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Sequence for pTet inverting regulator driven by the TetR protein.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: BBa_R0040 TetR-Regulated Promoter is based on a cI promoter. It has been modified to include two TetR binding sites and the BioBrick standard assembly head and tail restriction sites.
star: true
creator: June Rhee, Connie Tao, Ty Thomson, Louis Waldman
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -35
sequence annotation: TetR 2
sequence annotation: -10
sequence annotation: BBa_R0040
sequence annotation: TetR 1
sequence: https://synbiohub.org/public/igem/BBa_R0040_sequence/1

-35
type: SequenceAnnotation
persistent identity: annotation1986785
display id: annotation1986785
version: 1
top level: p(tetR)
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986785/range1986785/1

https://synbiohub.org/public/igem/BBa_R0040/annotation1986785/range1986785/1
type: Range
persistent identity: range1986785
display id: range1986785
version: 1
top level: p(tetR)
direction: direction/forward
start: 20
end: 25
orientation: inline

TetR 2
type: SequenceAnnotation
persistent identity: annotation1986786
display id: annotation1986786
version: 1
top level: p(tetR)
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986786/range1986786/1

https://synbiohub.org/public/igem/BBa_R0040/annotation1986786/range1986786/1
type: Range
persistent identity: range1986786
display id: range1986786
version: 1
top level: p(tetR)
direction: direction/forward
start: 26
end: 44
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1986787
display id: annotation1986787
version: 1
top level: p(tetR)
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986787/range1986787/1

https://synbiohub.org/public/igem/BBa_R0040/annotation1986787/range1986787/1
type: Range
persistent identity: range1986787
display id: range1986787
version: 1
top level: p(tetR)
direction: direction/forward
start: 43
end: 48
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1986784
display id: annotation1986784
version: 1
top level: p(tetR)
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986784/range1986784/1

https://synbiohub.org/public/igem/BBa_R0040/annotation1986784/range1986784/1
type: Range
persistent identity: range1986784
display id: range1986784
version: 1
top level: p(tetR)
direction: direction/forward
start: 1
end: 54
orientation: inline

TetR 1
type: SequenceAnnotation
persistent identity: annotation1986783
display id: annotation1986783
version: 1
top level: p(tetR)
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986783/range1986783/1

https://synbiohub.org/public/igem/BBa_R0040/annotation1986783/range1986783/1
type: Range
persistent identity: range1986783
display id: range1986783
version: 1
top level: p(tetR)
direction: direction/forward
start: 1
end: 19
orientation: inline

penI
type: ComponentDefinition
persistent identity: BBa C0074
display id: BBa_C0074
version: 1
was generated by: iGEM to SBOL conversion
description: penI repressor from Bacillus licheniformis (+LVA)
created: 2004-01-27T12:00:00Z
modified: 2015-08-31T04:07:23Z
mutable provenance: bacillus licheniformis
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: -- No description --
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Since penI is being ported from Bacillus Licheniformis, the sequence was changed within the coding region to reflect codon usage in E.Coli K12. Codons were mapped to the codon of the same usage rank in E.Coli.
star: true
creator: crackdots
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: penI
sequence annotation: LVA
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C0074_sequence/1

penI
type: SequenceAnnotation
persistent identity: annotation302638
display id: annotation302638
version: 1
top level: penI
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_C0074/annotation302638/range302638/1

https://synbiohub.org/public/igem/BBa_C0074/annotation302638/range302638/1
type: Range
persistent identity: range302638
display id: range302638
version: 1
top level: penI
direction: direction/forward
start: 1
end: 387
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation306563
display id: annotation306563
version: 1
top level: penI
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_C0074/annotation306563/range306563/1

https://synbiohub.org/public/igem/BBa_C0074/annotation306563/range306563/1
type: Range
persistent identity: range306563
display id: range306563
version: 1
top level: penI
direction: direction/forward
start: 385
end: 417
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2214009
display id: annotation2214009
version: 1
top level: penI
role: SO:0000807
role: feature/barcode
location: https://synbiohub.org/public/igem/BBa_C0074/annotation2214009/range2214009/1

https://synbiohub.org/public/igem/BBa_C0074/annotation2214009/range2214009/1
type: Range
persistent identity: range2214009
display id: range2214009
version: 1
top level: penI
direction: direction/forward
start: 424
end: 448
orientation: inline

mnt (w)
type: ComponentDefinition
persistent identity: BBa C0073
display id: BBa_C0073
version: 1
was generated by: iGEM to SBOL conversion
description: mnt repressor (weak) from Salmonella phage P22 (+LVA)
created: 2004-01-27T12:00:00Z
modified: 2015-08-31T04:07:23Z
mutable provenance: enterobacteriophage p22
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: -- No description --
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: dimeric repressor from enterobacteriophage p22 with dissociation constant of 3*10^-8. sequence was translated with the E.Coli K12 codon usage table, and nucleotides 95-97 was changed from AAT to AAC to avoid the cut site GAATTC (94-99). the mutation R10->K10 (ctg->aaa) decreases the binding affinity of the mnt protein to its operator by approximately 12,000 fold (Kd: 2.5e-12 to 3e-8)
star: true
creator: crackdots
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Arg->Lys (ctg)
sequence annotation: LVA
sequence annotation: Help:Barcodes
sequence annotation: mnt
sequence annotation: T
sequence: https://synbiohub.org/public/igem/BBa_C0073_sequence/1

Arg->Lys (ctg)
type: SequenceAnnotation
persistent identity: annotation308213
display id: annotation308213
version: 1
top level: mnt (w)
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_C0073/annotation308213/range308213/1

https://synbiohub.org/public/igem/BBa_C0073/annotation308213/range308213/1
type: Range
persistent identity: range308213
display id: range308213
version: 1
top level: mnt (w)
direction: direction/forward
start: 31
end: 33
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation306535
display id: annotation306535
version: 1
top level: mnt (w)
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_C0073/annotation306535/range306535/1

https://synbiohub.org/public/igem/BBa_C0073/annotation306535/range306535/1
type: Range
persistent identity: range306535
display id: range306535
version: 1
top level: mnt (w)
direction: direction/forward
start: 250
end: 282
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2214006
display id: annotation2214006
version: 1
top level: mnt (w)
role: SO:0000807
role: feature/barcode
location: https://synbiohub.org/public/igem/BBa_C0073/annotation2214006/range2214006/1

https://synbiohub.org/public/igem/BBa_C0073/annotation2214006/range2214006/1
type: Range
persistent identity: range2214006
display id: range2214006
version: 1
top level: mnt (w)
direction: direction/forward
start: 289
end: 313
orientation: inline

mnt
type: SequenceAnnotation
persistent identity: annotation302631
display id: annotation302631
version: 1
top level: mnt (w)
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_C0073/annotation302631/range302631/1

https://synbiohub.org/public/igem/BBa_C0073/annotation302631/range302631/1
type: Range
persistent identity: range302631
display id: range302631
version: 1
top level: mnt (w)
direction: direction/forward
start: 1
end: 249
orientation: inline

T
type: SequenceAnnotation
persistent identity: annotation308457
display id: annotation308457
version: 1
top level: mnt (w)
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_C0073/annotation308457/range308457/1

https://synbiohub.org/public/igem/BBa_C0073/annotation308457/range308457/1
type: Range
persistent identity: range308457
display id: range308457
version: 1
top level: mnt (w)
direction: direction/forward
start: 95
end: 97
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

BBa_B0030
type: ComponentDefinition
persistent identity: BBa B0030
display id: BBa_B0030
version: 1
was generated by: iGEM to SBOL conversion
description: RBS.1 (strong) -- modified from R. Weiss
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>.
discontinued: false
dominant: true
experience: experience/Works
group u list: _44_46_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("orig" in figure 4-14 of Ron Weiss thesis). No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: BBa_B0030
sequence annotation: RBS
sequence annotation: RBS-1\Strong
sequence: https://synbiohub.org/public/igem/BBa_B0030_sequence/1

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation7025
display id: annotation7025
version: 1
top level: BBa_B0030
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_B0030/annotation7025/range7025/1

https://synbiohub.org/public/igem/BBa_B0030/annotation7025/range7025/1
type: Range
persistent identity: range7025
display id: range7025
version: 1
top level: BBa_B0030
direction: direction/forward
start: 1
end: 15
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation1702
display id: annotation1702
version: 1
top level: BBa_B0030
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_B0030/annotation1702/range1702/1

https://synbiohub.org/public/igem/BBa_B0030/annotation1702/range1702/1
type: Range
persistent identity: range1702
display id: range1702
version: 1
top level: BBa_B0030
direction: direction/forward
start: 8
end: 12
orientation: inline

RBS-1\Strong
type: SequenceAnnotation
persistent identity: annotation1701
display id: annotation1701
version: 1
top level: BBa_B0030
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_B0030/annotation1701/range1701/1

https://synbiohub.org/public/igem/BBa_B0030/annotation1701/range1701/1
type: Range
persistent identity: range1701
display id: range1701
version: 1
top level: BBa_B0030
direction: direction/forward
start: 1
end: 15
orientation: inline

lacI
type: ComponentDefinition
persistent identity: BBa Q04121
display id: BBa_Q04121
version: 1
was generated by: iGEM to SBOL conversion
description: QPI (B0034.C0012.B0015.R0011)
created: 2003-12-02T12:00:00Z
modified: 2015-05-08T01:14:13Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: LacI QPI with strong RBS, hybrid promoter
discontinued: false
dominant: false
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Caitlin Conboy
type: DnaRegion
role: SO:0000804
role: partType/Inverter
component: BBa_R0011
component: BBa_B0034
component: BBa_B0015
component: BBa_C0012
sequence annotation: BBa_B0015
sequence annotation: BBa_R0011
sequence annotation: BBa_C0012
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_Q04121_sequence/1

BBa_R0011
type: Component
persistent identity: component2221146
display id: component2221146
version: 1
top level: lacI
access: public
definition: lacI+pL

BBa_B0034
type: Component
persistent identity: component2221134
display id: component2221134
version: 1
top level: lacI
access: public
definition: BBa_B0034

BBa_B0015
type: Component
persistent identity: component2221145
display id: component2221145
version: 1
top level: lacI
access: public
definition: BBa_B0015

BBa_C0012
type: Component
persistent identity: component2221136
display id: component2221136
version: 1
top level: lacI
access: public
definition: lacI

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2221145
display id: annotation2221145
version: 1
top level: lacI
location: https://synbiohub.org/public/igem/BBa_Q04121/annotation2221145/range2221145/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_Q04121/annotation2221145/range2221145/1
type: Range
persistent identity: range2221145
display id: range2221145
version: 1
top level: lacI
direction: direction/reverse
start: 1180
end: 1308
orientation: inline

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation2221146
display id: annotation2221146
version: 1
top level: lacI
location: https://synbiohub.org/public/igem/BBa_Q04121/annotation2221146/range2221146/1
component: BBa_R0011

https://synbiohub.org/public/igem/BBa_Q04121/annotation2221146/range2221146/1
type: Range
persistent identity: range2221146
display id: range2221146
version: 1
top level: lacI
direction: direction/forward
start: 1317
end: 1370
orientation: inline

BBa_C0012
type: SequenceAnnotation
persistent identity: annotation2221136
display id: annotation2221136
version: 1
top level: lacI
location: https://synbiohub.org/public/igem/BBa_Q04121/annotation2221136/range2221136/1
component: BBa_C0012

https://synbiohub.org/public/igem/BBa_Q04121/annotation2221136/range2221136/1
type: Range
persistent identity: range2221136
display id: range2221136
version: 1
top level: lacI
direction: direction/forward
start: 19
end: 1146
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2221134
display id: annotation2221134
version: 1
top level: lacI
location: https://synbiohub.org/public/igem/BBa_Q04121/annotation2221134/range2221134/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_Q04121/annotation2221134/range2221134/1
type: Range
persistent identity: range2221134
display id: range2221134
version: 1
top level: lacI
direction: direction/reverse
start: 1
end: 12
orientation: inline

cI lam
type: ComponentDefinition
persistent identity: BBa I9201
display id: BBa_I9201
version: 1
was generated by: iGEM to SBOL conversion
description: lambda cI operator/binding site
created: 2004-01-27T12:00:00Z
modified: 2015-08-31T04:08:11Z
mutable provenance: lambda bacteriophage
owned by: james
owned by: myers
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: operator sites: O1, O2, and O3
star: false
creator: crackdots
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: cI lambda
sequence: https://synbiohub.org/public/igem/BBa_I9201_sequence/1

cI lambda
type: SequenceAnnotation
persistent identity: annotation302665
display id: annotation302665
version: 1
top level: cI lam
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_I9201/annotation302665/range302665/1

https://synbiohub.org/public/igem/BBa_I9201/annotation302665/range302665/1
type: Range
persistent identity: range302665
display id: range302665
version: 1
top level: cI lam
direction: direction/forward
start: 1
end: 82
orientation: inline

tetR
type: ComponentDefinition
persistent identity: BBa C0040
display id: BBa_C0040
version: 1
was generated by: iGEM to SBOL conversion
description: tetracycline repressor from transposon Tn10 (+LVA)
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:23Z
mutable provenance: Elowitz, M. B. Transport, Assembly, and Dynamics in Systems of Interacting Proteins. Thesis, Princeton Univ., Princeton (1999)
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Coding region for the TetR protein without the Ribosome Binding Site. Modified with an LVA tail for rapid degradation of the protein and faster fall time for the emission. TetR binds to the pTet regulator (BBa_R0040). aTc (anhydrotetracycline) binds to TetR and inhibits its operation.
discontinued: false
dominant: true
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: References (unparsed) here: Elowitz, M. B. Transport, Assembly, and Dynamics in Systems of Interacting Proteins. Thesis, Princeton Univ., Princeton (1999). Lutz R, Bujard H., Independent and tight regulation of transcriptional units in Escherichia coli via the LacR/O, the TetR/O and AraC/I1-I2 regulatory elements. Nucleic Acids Res. 1997 Mar 15;25(6):1203-10. PMID: 9092630 References (unparsed) here: Elowitz, M. B. Transport, Assembly, and Dynamics in Systems of Interacting Proteins. Thesis, Princeton Univ., Princeton (1999). Lutz R, Bujard H., Independent and tight regulation of transcriptional units in Escherichia coli via the LacR/O, the TetR/O and AraC/I1-I2 regulatory elements. Nucleic Acids Res. 1997 Mar 15;25(6):1203-10. PMID: 9092630 BBa_C0040 TetR Protein is based on the TetR sequence from Elowitz's repressilator. It has been modified to include a rapid degradation LVA tail, and includes the BioBrick standard assembly head and tail restriction sites. The RBS has been removed. The stop codon has been changed from TAA to a double stop codon TAATAA.
star: true
creator: June Rhee, Connie Tao, Ty Thomson, Louis Waldman.
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Help:Barcodes
sequence annotation: tetR
sequence annotation: SsrA
sequence: https://synbiohub.org/public/igem/BBa_C0040_sequence/1

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213989
display id: annotation2213989
version: 1
top level: tetR
role: SO:0000807
role: feature/barcode
location: https://synbiohub.org/public/igem/BBa_C0040/annotation2213989/range2213989/1

https://synbiohub.org/public/igem/BBa_C0040/annotation2213989/range2213989/1
type: Range
persistent identity: range2213989
display id: range2213989
version: 1
top level: tetR
direction: direction/forward
start: 661
end: 685
orientation: inline

tetR
type: SequenceAnnotation
persistent identity: annotation23329
display id: annotation23329
version: 1
top level: tetR
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_C0040/annotation23329/range23329/1

https://synbiohub.org/public/igem/BBa_C0040/annotation23329/range23329/1
type: Range
persistent identity: range23329
display id: range23329
version: 1
top level: tetR
direction: direction/forward
start: 4
end: 620
orientation: inline

SsrA
type: SequenceAnnotation
persistent identity: annotation23330
display id: annotation23330
version: 1
top level: tetR
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_C0040/annotation23330/range23330/1

https://synbiohub.org/public/igem/BBa_C0040/annotation23330/range23330/1
type: Range
persistent identity: range23330
display id: range23330
version: 1
top level: tetR
direction: direction/forward
start: 621
end: 654
orientation: inline

cI
type: ComponentDefinition
persistent identity: BBa I9200
display id: BBa_I9200
version: 1
was generated by: iGEM to SBOL conversion
description: cI lambda
created: 2004-01-27T12:00:00Z
modified: 2015-08-31T04:08:11Z
mutable provenance: lambda bacteriophage
owned by: james
owned by: myers
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: cI sequence slightly different from part C0051. NCBI locus: NC_001416
star: false
creator: crackdots
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: cI lambda
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_I9200_sequence/1

cI lambda
type: SequenceAnnotation
persistent identity: annotation302655
display id: annotation302655
version: 1
top level: cI
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_I9200/annotation302655/range302655/1

https://synbiohub.org/public/igem/BBa_I9200/annotation302655/range302655/1
type: Range
persistent identity: range302655
display id: range302655
version: 1
top level: cI
direction: direction/forward
start: 1
end: 717
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2214008
display id: annotation2214008
version: 1
top level: cI
role: SO:0000807
role: feature/barcode
location: https://synbiohub.org/public/igem/BBa_I9200/annotation2214008/range2214008/1

https://synbiohub.org/public/igem/BBa_I9200/annotation2214008/range2214008/1
type: Range
persistent identity: range2214008
display id: range2214008
version: 1
top level: cI
direction: direction/forward
start: 718
end: 742
orientation: inline

tetR inver
type: ComponentDefinition
persistent identity: BBa Q04401
display id: BBa_Q04401
version: 1
was generated by: iGEM to SBOL conversion
description: TetR-derived transcriptional inverter
created: 2006-05-10T11:00:00Z
modified: 2015-05-08T01:14:13Z
owned by: james
owned by: myers
mutable description: Currently entered as a placeholder. This is a modified version of the tetR inverter Q04400 with a single base switch in the RBS: AAAGAGGAGAAA to AAAGAGGGGAAA
discontinued: false
dominant: false
experience: experience/Works
group u list: _11_
m user id: 0
owner id: 253
owning group id: 6
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Josh Michener
type: DnaRegion
role: SO:0000804
role: partType/Inverter
component: BBa_R0040
component: BBa_B0064
component: BBa_C0040
component: BBa_B0015
sequence annotation: BBa_R0040
sequence annotation: BBa_C0040
sequence annotation: BBa_B0015
sequence annotation: BBa_B0064
sequence: https://synbiohub.org/public/igem/BBa_Q04401_sequence/1

BBa_R0040
type: Component
persistent identity: component2251471
display id: component2251471
version: 1
top level: tetR inver
access: public
definition: p(tetR)

BBa_B0064
type: Component
persistent identity: component2251459
display id: component2251459
version: 1
top level: tetR inver
access: public
definition: BBa_B0064

BBa_C0040
type: Component
persistent identity: component2251463
display id: component2251463
version: 1
top level: tetR inver
access: public
definition: tetR

BBa_B0015
type: Component
persistent identity: component2251470
display id: component2251470
version: 1
top level: tetR inver
access: public
definition: BBa_B0015

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation2251471
display id: annotation2251471
version: 1
top level: tetR inver
location: https://synbiohub.org/public/igem/BBa_Q04401/annotation2251471/range2251471/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_Q04401/annotation2251471/range2251471/1
type: Range
persistent identity: range2251471
display id: range2251471
version: 1
top level: tetR inver
direction: direction/forward
start: 849
end: 902
orientation: inline

BBa_C0040
type: SequenceAnnotation
persistent identity: annotation2251463
display id: annotation2251463
version: 1
top level: tetR inver
location: https://synbiohub.org/public/igem/BBa_Q04401/annotation2251463/range2251463/1
component: BBa_C0040

https://synbiohub.org/public/igem/BBa_Q04401/annotation2251463/range2251463/1
type: Range
persistent identity: range2251463
display id: range2251463
version: 1
top level: tetR inver
direction: direction/reverse
start: 19
end: 703
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2251470
display id: annotation2251470
version: 1
top level: tetR inver
location: https://synbiohub.org/public/igem/BBa_Q04401/annotation2251470/range2251470/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_Q04401/annotation2251470/range2251470/1
type: Range
persistent identity: range2251470
display id: range2251470
version: 1
top level: tetR inver
direction: direction/reverse
start: 712
end: 840
orientation: inline

BBa_B0064
type: SequenceAnnotation
persistent identity: annotation2251459
display id: annotation2251459
version: 1
top level: tetR inver
location: https://synbiohub.org/public/igem/BBa_Q04401/annotation2251459/range2251459/1
component: BBa_B0064

https://synbiohub.org/public/igem/BBa_Q04401/annotation2251459/range2251459/1
type: Range
persistent identity: range2251459
display id: range2251459
version: 1
top level: tetR inver
direction: direction/reverse
start: 1
end: 12
orientation: inline

LacI
type: ComponentDefinition
persistent identity: BBa R0010
display id: BBa_R0010
version: 1
was generated by: iGEM to SBOL conversion
description: promoter (lacI regulated)
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: The Plac insert was PCR'd from the MG1655 strain of E.coli K12.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) The pLac regulatory region is a 243 base-pair sequence with standard BioBrick prefix and suffix sections on its ends. It contains two protein binding sites: CAP, which is generally present in E.coli and is assocciated with cell health and availability of glucose., and LacI, the Lac inhibitor <bb_part>BBa_C0010</bb_part> which binds in an dimerized cooperative manner to inhibit the transcription of the protein that follows. In the presence of lactose or IPTG, an analog of lactose, LacI is unable to correctly bind and inhibit transcription. This allows <bb_part>BBa_R0010</bb_part> to be used as a inverter or as a detector of lactose or IPTG.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and this regulator. This part is incompatible with environments containing lactose or lactose analogs.
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: start
sequence annotation: LacI binding site
sequence annotation: end of LacI coding region (inactive)
sequence annotation: CAP binding site
sequence annotation: -10
sequence annotation: -35
sequence annotation: BBa_R0010
sequence: https://synbiohub.org/public/igem/BBa_R0010_sequence/1

start
type: SequenceAnnotation
persistent identity: annotation1961227
display id: annotation1961227
version: 1
top level: LacI
role: feature/start
role: SO:0000318
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961227/range1961227/1

https://synbiohub.org/public/igem/BBa_R0010/annotation1961227/range1961227/1
type: Range
persistent identity: range1961227
display id: range1961227
version: 1
top level: LacI
direction: direction/forward
start: 173
end: 173
orientation: inline

LacI binding site
type: SequenceAnnotation
persistent identity: annotation1961226
display id: annotation1961226
version: 1
top level: LacI
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961226/range1961226/1

https://synbiohub.org/public/igem/BBa_R0010/annotation1961226/range1961226/1
type: Range
persistent identity: range1961226
display id: range1961226
version: 1
top level: LacI
direction: direction/forward
start: 166
end: 200
orientation: inline

end of LacI coding region (inactive)
type: SequenceAnnotation
persistent identity: annotation1961221
display id: annotation1961221
version: 1
top level: LacI
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961221/range1961221/1

https://synbiohub.org/public/igem/BBa_R0010/annotation1961221/range1961221/1
type: Range
persistent identity: range1961221
display id: range1961221
version: 1
top level: LacI
direction: direction/forward
start: 1
end: 88
orientation: inline

CAP binding site
type: SequenceAnnotation
persistent identity: annotation1961223
display id: annotation1961223
version: 1
top level: LacI
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961223/range1961223/1

https://synbiohub.org/public/igem/BBa_R0010/annotation1961223/range1961223/1
type: Range
persistent identity: range1961223
display id: range1961223
version: 1
top level: LacI
direction: direction/forward
start: 89
end: 126
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1961225
display id: annotation1961225
version: 1
top level: LacI
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961225/range1961225/1

https://synbiohub.org/public/igem/BBa_R0010/annotation1961225/range1961225/1
type: Range
persistent identity: range1961225
display id: range1961225
version: 1
top level: LacI
direction: direction/forward
start: 161
end: 166
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1961224
display id: annotation1961224
version: 1
top level: LacI
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961224/range1961224/1

https://synbiohub.org/public/igem/BBa_R0010/annotation1961224/range1961224/1
type: Range
persistent identity: range1961224
display id: range1961224
version: 1
top level: LacI
direction: direction/forward
start: 137
end: 142
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation1961222
display id: annotation1961222
version: 1
top level: LacI
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961222/range1961222/1

https://synbiohub.org/public/igem/BBa_R0010/annotation1961222/range1961222/1
type: Range
persistent identity: range1961222
display id: range1961222
version: 1
top level: LacI
direction: direction/forward
start: 1
end: 200
orientation: inline

cI TP901
type: ComponentDefinition
persistent identity: BBa C0075
display id: BBa_C0075
version: 1
was generated by: iGEM to SBOL conversion
description: TP901 cI repressor from phage TP901-1 (+LVA)
created: 2004-01-27T12:00:00Z
modified: 2015-08-31T04:07:23Z
mutable provenance: TP901-1
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: -- No description --
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Dimeric repressor from bacteriophage TP901-1 with dissociation constant of 2*10^-9. Sequence was translated with the E.Coli K12 codon usage table, and nucleotides 84-86 was changed from TTC to TTT to avoid the cut site GAATTC (81-86). Bronsted, L., Hammer, K., Johansen, A.H., 2003. Identification of operator sites of the CI repressor of the phage TP901-1: evolutionary link to the other phages. Virology 311, 144-156. cI sequence slightly different from part C0051. NCBI locus: NC_001416
star: true
creator: crackdots
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: LVA
sequence annotation: Help:Barcodes
sequence annotation: cI tp901
sequence: https://synbiohub.org/public/igem/BBa_C0075_sequence/1

LVA
type: SequenceAnnotation
persistent identity: annotation306574
display id: annotation306574
version: 1
top level: cI TP901
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_C0075/annotation306574/range306574/1

https://synbiohub.org/public/igem/BBa_C0075/annotation306574/range306574/1
type: Range
persistent identity: range306574
display id: range306574
version: 1
top level: cI TP901
direction: direction/forward
start: 541
end: 573
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2214007
display id: annotation2214007
version: 1
top level: cI TP901
role: SO:0000807
role: feature/barcode
location: https://synbiohub.org/public/igem/BBa_C0075/annotation2214007/range2214007/1

https://synbiohub.org/public/igem/BBa_C0075/annotation2214007/range2214007/1
type: Range
persistent identity: range2214007
display id: range2214007
version: 1
top level: cI TP901
direction: direction/forward
start: 580
end: 604
orientation: inline

cI tp901
type: SequenceAnnotation
persistent identity: annotation302645
display id: annotation302645
version: 1
top level: cI TP901
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_C0075/annotation302645/range302645/1

https://synbiohub.org/public/igem/BBa_C0075/annotation302645/range302645/1
type: Range
persistent identity: range302645
display id: range302645
version: 1
top level: cI TP901
direction: direction/forward
start: 1
end: 540
orientation: inline

cI lam
type: ComponentDefinition
persistent identity: BBa R0051
display id: BBa_R0051
version: 1
was generated by: iGEM to SBOL conversion
description: promoter (lambda cI regulated)
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: A synthetic oscillatory network of transcriptional regulators , Elowitz M.B. , Leibler S., Nature(403),335-38: 2000
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: The cI regulated promoter is based on the pR promtoer from bacteriohage lambda. The promoter has two two DNA binding sites for lambda cI repressor <bb_part>BBa_C0051</bb_part>. cI binding results in repression of transcription. The specific sequence used here is based on the cI repressible promoter used in the Elowitz repressilator (and references therein).
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: In order to address concerns about the promoter transcribing in the reverse direction, we have removed the -35 and -10 signals responsible for the promoter activity in the reverse direction. (More details needed here! DE, 2/24/03) Incompatible with host expressing cI repressor.
star: true
creator: Vinay S Mahajan, Brian Chow, Peter Carr, Voichita Marinescu and Alexander D. Wissner-Gross
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -10
sequence annotation: OR1
sequence annotation: -35
sequence annotation: BBa_R0051
sequence annotation: OR2
sequence: https://synbiohub.org/public/igem/BBa_R0051_sequence/1

-10
type: SequenceAnnotation
persistent identity: annotation2022
display id: annotation2022
version: 1
top level: cI lam
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0051/annotation2022/range2022/1

https://synbiohub.org/public/igem/BBa_R0051/annotation2022/range2022/1
type: Range
persistent identity: range2022
display id: range2022
version: 1
top level: cI lam
direction: direction/forward
start: 38
end: 43
orientation: inline

OR1
type: SequenceAnnotation
persistent identity: annotation2024
display id: annotation2024
version: 1
top level: cI lam
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0051/annotation2024/range2024/1

https://synbiohub.org/public/igem/BBa_R0051/annotation2024/range2024/1
type: Range
persistent identity: range2024
display id: range2024
version: 1
top level: cI lam
direction: direction/forward
start: 25
end: 41
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2023
display id: annotation2023
version: 1
top level: cI lam
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0051/annotation2023/range2023/1

https://synbiohub.org/public/igem/BBa_R0051/annotation2023/range2023/1
type: Range
persistent identity: range2023
display id: range2023
version: 1
top level: cI lam
direction: direction/forward
start: 15
end: 20
orientation: inline

BBa_R0051
type: SequenceAnnotation
persistent identity: annotation7067
display id: annotation7067
version: 1
top level: cI lam
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_R0051/annotation7067/range7067/1

https://synbiohub.org/public/igem/BBa_R0051/annotation7067/range7067/1
type: Range
persistent identity: range7067
display id: range7067
version: 1
top level: cI lam
direction: direction/forward
start: 1
end: 49
orientation: inline

OR2
type: SequenceAnnotation
persistent identity: annotation2025
display id: annotation2025
version: 1
top level: cI lam
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0051/annotation2025/range2025/1

https://synbiohub.org/public/igem/BBa_R0051/annotation2025/range2025/1
type: Range
persistent identity: range2025
display id: range2025
version: 1
top level: cI lam
direction: direction/forward
start: 1
end: 17
orientation: inline

BBa_Q00400
type: ComponentDefinition
persistent identity: BBa Q00400
display id: BBa_Q00400
version: 1
was generated by: iGEM to SBOL conversion
description: Tet
created: 2004-07-19T11:00:00Z
modified: 2015-05-08T01:14:13Z
owned by: james
owned by: myers
mutable description: Tet QPI with B0030 which according to preliminary experiments is a "strong" strength RBS.
discontinued: false
dominant: false
experience: experience/None
group u list: _6_
m user id: 0
owner id: 101
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: mit
type: DnaRegion
role: SO:0000804
role: partType/Inverter
component: BBa_B0010
component: BBa_B0012
component: BBa_R0040
component: BBa_C0040
component: BBa_B0030
sequence annotation: BBa_B0012
sequence annotation: BBa_B0030
sequence annotation: BBa_R0040
sequence annotation: BBa_B0010
sequence annotation: BBa_C0040
sequence: https://synbiohub.org/public/igem/BBa_Q00400_sequence/1

BBa_B0010
type: Component
persistent identity: component951632
display id: component951632
version: 1
top level: BBa_Q00400
access: public
definition: BBa_B0010

BBa_B0012
type: Component
persistent identity: component951642
display id: component951642
version: 1
top level: BBa_Q00400
access: public
definition: BBa_B0012

BBa_R0040
type: Component
persistent identity: component951657
display id: component951657
version: 1
top level: BBa_Q00400
access: public
definition: p(tetR)

BBa_C0040
type: Component
persistent identity: component951626
display id: component951626
version: 1
top level: BBa_Q00400
access: public
definition: tetR

BBa_B0030
type: Component
persistent identity: component951615
display id: component951615
version: 1
top level: BBa_Q00400
access: public
definition: BBa_B0030

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation951642
display id: annotation951642
version: 1
top level: BBa_Q00400
location: https://synbiohub.org/public/igem/BBa_Q00400/annotation951642/range951642/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_Q00400/annotation951642/range951642/1
type: Range
persistent identity: range951642
display id: range951642
version: 1
top level: BBa_Q00400
direction: direction/forward
start: 803
end: 843
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation951615
display id: annotation951615
version: 1
top level: BBa_Q00400
location: https://synbiohub.org/public/igem/BBa_Q00400/annotation951615/range951615/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_Q00400/annotation951615/range951615/1
type: Range
persistent identity: range951615
display id: range951615
version: 1
top level: BBa_Q00400
direction: direction/forward
start: 1
end: 15
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation951657
display id: annotation951657
version: 1
top level: BBa_Q00400
location: https://synbiohub.org/public/igem/BBa_Q00400/annotation951657/range951657/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_Q00400/annotation951657/range951657/1
type: Range
persistent identity: range951657
display id: range951657
version: 1
top level: BBa_Q00400
direction: direction/forward
start: 852
end: 905
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation951632
display id: annotation951632
version: 1
top level: BBa_Q00400
location: https://synbiohub.org/public/igem/BBa_Q00400/annotation951632/range951632/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_Q00400/annotation951632/range951632/1
type: Range
persistent identity: range951632
display id: range951632
version: 1
top level: BBa_Q00400
direction: direction/forward
start: 715
end: 794
orientation: inline

BBa_C0040
type: SequenceAnnotation
persistent identity: annotation951626
display id: annotation951626
version: 1
top level: BBa_Q00400
location: https://synbiohub.org/public/igem/BBa_Q00400/annotation951626/range951626/1
component: BBa_C0040

https://synbiohub.org/public/igem/BBa_Q00400/annotation951626/range951626/1
type: Range
persistent identity: range951626
display id: range951626
version: 1
top level: BBa_Q00400
direction: direction/forward
start: 22
end: 681
orientation: inline

BBa_I9001
type: ComponentDefinition
persistent identity: BBa I9001
display id: BBa_I9001
version: 1
was generated by: iGEM to SBOL conversion
description: QPI (mnt) B0034.C0073.B0015.R0073
created: 2004-01-27T12:00:00Z
modified: 2015-08-31T04:08:11Z
owned by: james
owned by: myers
discontinued: false
dominant: true
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: crackdots
type: DnaRegion
role: SO:0000804
role: partType/Inverter
component: BBa_C0073
component: BBa_B0010
component: BBa_B0012
component: BBa_B0034
component: BBa_R0073
sequence annotation: BBa_C0073
sequence annotation: BBa_B0012
sequence annotation: BBa_R0073
sequence annotation: BBa_B0010
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_I9001_sequence/1

BBa_C0073
type: Component
persistent identity: component951123
display id: component951123
version: 1
top level: BBa_I9001
access: public
definition: mnt (w)

BBa_B0010
type: Component
persistent identity: component951129
display id: component951129
version: 1
top level: BBa_I9001
access: public
definition: BBa_B0010

BBa_B0012
type: Component
persistent identity: component951139
display id: component951139
version: 1
top level: BBa_I9001
access: public
definition: BBa_B0012

BBa_B0034
type: Component
persistent identity: component951107
display id: component951107
version: 1
top level: BBa_I9001
access: public
definition: BBa_B0034

BBa_R0073
type: Component
persistent identity: component951156
display id: component951156
version: 1
top level: BBa_I9001
access: public
definition: Mnt

BBa_C0073
type: SequenceAnnotation
persistent identity: annotation951123
display id: annotation951123
version: 1
top level: BBa_I9001
location: https://synbiohub.org/public/igem/BBa_I9001/annotation951123/range951123/1
component: BBa_C0073

https://synbiohub.org/public/igem/BBa_I9001/annotation951123/range951123/1
type: Range
persistent identity: range951123
display id: range951123
version: 1
top level: BBa_I9001
direction: direction/forward
start: 19
end: 306
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation951139
display id: annotation951139
version: 1
top level: BBa_I9001
location: https://synbiohub.org/public/igem/BBa_I9001/annotation951139/range951139/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I9001/annotation951139/range951139/1
type: Range
persistent identity: range951139
display id: range951139
version: 1
top level: BBa_I9001
direction: direction/forward
start: 428
end: 468
orientation: inline

BBa_R0073
type: SequenceAnnotation
persistent identity: annotation951156
display id: annotation951156
version: 1
top level: BBa_I9001
location: https://synbiohub.org/public/igem/BBa_I9001/annotation951156/range951156/1
component: BBa_R0073

https://synbiohub.org/public/igem/BBa_I9001/annotation951156/range951156/1
type: Range
persistent identity: range951156
display id: range951156
version: 1
top level: BBa_I9001
direction: direction/forward
start: 477
end: 543
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation951129
display id: annotation951129
version: 1
top level: BBa_I9001
location: https://synbiohub.org/public/igem/BBa_I9001/annotation951129/range951129/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I9001/annotation951129/range951129/1
type: Range
persistent identity: range951129
display id: range951129
version: 1
top level: BBa_I9001
direction: direction/forward
start: 340
end: 419
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation951107
display id: annotation951107
version: 1
top level: BBa_I9001
location: https://synbiohub.org/public/igem/BBa_I9001/annotation951107/range951107/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I9001/annotation951107/range951107/1
type: Range
persistent identity: range951107
display id: range951107
version: 1
top level: BBa_I9001
direction: direction/forward
start: 1
end: 12
orientation: inline

https://synbiohub.org/public/igem/BBa_R0074_sequence/1
type: Sequence
persistent identity: BBa R0074 sequence
display id: BBa_R0074_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcatttccaaccgaaaaaaggcttgcatttaaatcttacatatgtaatactttcaaagactacatttgtaagatttg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_Q09400_sequence/1
type: Sequence
persistent identity: BBa Q09400 sequence
display id: BBa_Q09400_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacacaggaaaggcctcgatgtactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0010_sequence/1
type: Sequence
persistent identity: BBa R0010 sequence
display id: BBa_R0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_Q01121_sequence/1
type: Sequence
persistent identity: BBa Q01121 sequence
display id: BBa_Q01121_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacacaggaaacctactagatggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcaggctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagaattgtgagcggataacaattgacattgtgagcggataacaagatactgagcaca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0073_sequence/1
type: Sequence
persistent identity: BBa C0073 sequence
display id: BBa_C0073_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggcccgggatgatcctcacttcaattttaaaatgccaatggaagtaagagagaaattgaaatttagagcagaggcaaacggacggagcatgaactctgagcttttgcaaatcgtacaagatgccctaagcaaaccgtcaccagtcactgggtaccgcaatgatgcggaacgactcgccgatgagcagagcgagttagtgaagaagatggtcttcgatacactgaaggatctttataaaaaaaccaccgctgcaaacgacgaaaactacgctttagtagcttaataacgctgatagtgctagtgtagatcgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J06800_sequence/1
type: Sequence
persistent identity: BBa J06800 sequence
display id: BBa_J06800_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactacgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcaggctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagaattgtgagcggataacaattgacattgtgagcggataacaagatactgagcaca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0051_sequence/1
type: Sequence
persistent identity: BBa R0051 sequence
display id: BBa_R0051_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: taacaccgtgcgtgttgactattttacctctggcggtgataatggttgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_Q04122_sequence/1
type: Sequence
persistent identity: BBa Q04122 sequence
display id: BBa_Q04122_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcaggctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagcaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_Q04401_sequence/1
type: Sequence
persistent identity: BBa Q04401 sequence
display id: BBa_Q04401_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggggaaatactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J06503_sequence/1
type: Sequence
persistent identity: BBa J06503 sequence
display id: BBa_J06503_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgagcacaaaaaagaaaccattaacacaagagcagcttgaggacgcacgtcgccttaaagcaatttatgaaaaaaagaaaaatgaacttggcttatcccaggaatctgtcgcagacaagatggggatggggcagtcaggcgttggtgctttatttaatggcatcaatgcattaaatgcttataacgccgcattgcttacaaaaattctcaaagttagcgttgaagaatttagcccttcaatcgccagagaaatctacgagatgtatgaagcggttagtatgcagccgtcacttagaagtgagtatgagtaccctgttttttctcatgttcaggcagggatgttctcacctaagcttagaacctttaccaaaggtgatgcggagagatgggtaagcacaaccaaaaaagccagtgattctgcattctggcttgaggttgaaggtaattccatgaccgcaccaacaggctccaagccaagctttcctgacggaatgttaattctcgttgaccctgagcaggctgttgagccaggtgatttctgcatagccagacttgggggtgatgagtttaccttcaagaaactgatcagggatagcggtcaggtgtttttacaaccactaaacccacagtacccaatgatcccatgcaatgagagttgttccgttgtggggaaagttatcgctagtcagtggcctgaagagacgtttggcgctgcaaacgacgaaaactacgctttagtagcttaataacgctgatagtgctagtgtagatcgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_Q04740_sequence/1
type: Sequence
persistent identity: BBa Q04740 sequence
display id: BBa_Q04740_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatgaaaaaaataccacagatttctgatgcggaactcgaagtcatgaaagtgatttggaagcattcttccattaatactaatgaggtaatcaaagagctcagtaaaacttcaacgtggagcccaaaaactattcagactatgctgctgcgccttatcaaaaaaggcgctctcaaccaccataaagaaggtcgggttttcgtttacacgcctaatatagacgaatcagattatatagaggttaagtcacactcatttctcaaccggttttacaatggtacattaaattccatggtactcaactttttggagaatgatcaactgtcgggagaagaaatcaatgaattgtatcagatactcgaagaacataagaaccgtaagaaggaagctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtcatttccaaccgaaaaaaggcttgcatttaaatcttacatatgtaatactttcaaagactacatttgtaagatttg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0073_sequence/1
type: Sequence
persistent identity: BBa R0073 sequence
display id: BBa_R0073_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ctcgaggtgagtgcacagtactaggtccacggtgacctagatctcctatagtgagtcgtattaattt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_Q04121_sequence/1
type: Sequence
persistent identity: BBa Q04121 sequence
display id: BBa_Q04121_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcaggctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagaattgtgagcggataacaattgacattgtgagcggataacaagatactgagcaca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_Q04510_sequence/1
type: Sequence
persistent identity: BBa Q04510 sequence
display id: BBa_Q04510_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatgagcacaaaaaagaaaccattaacacaagagcagcttgaggacgcacgtcgccttaaagcaatttatgaaaaaaagaaaaatgaacttggcttatcccaggaatctgtcgcagacaagatggggatggggcagtcaggcgttggtgctttatttaatggcatcaatgcattaaatgcttataacgccgcattgcttgcaaaaattctcaaagttagcgttgaagaatttagcccttcaatcgccagagaaatctacgagatgtatgaagcggttagtatgcagccgtcacttagaagtgagtatgagtaccctgttttttctcatgttcaggcagggatgttctcacctgagcttagaacctttaccaaaggtgatgcggagagatgggtaagcacaaccaaaaaagccagtgattctgcattctggcttgaggttgaaggtaattccatgaccgcaccaacaggctccaagccaagctttcctgacggaatgttaattctcgttgaccctgagcaggctgttgagccaggtgatttctgcatagccagacttgggggtgatgagtttaccttcaagaaactgatcagggatagcggtcaggtgtttttacaaccactaaacccacagtacccaatgatcccatgcaatgagagttgttccgttgtggggaaagttatcgctagtcagtggcctgaagagacgtttggcgctgcaaacgacgaaaactacgctttagtagcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtaacaccgtgcgtgttgactattttacctctggcggtgataatggttgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I9003_sequence/1
type: Sequence
persistent identity: BBa I9003 sequence
display id: BBa_I9003_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatgaaaaccgataccagtaacagattaaaacaaataatggcggaacgcaacctgaaacaggtggatatcttaaacttatcgattccatttcagaaaaaatttggtatcaagctgagtaaaagtacgctgtcgcagtatgtgaactctgtgcagtcgccagaccagaaccgcatctatttactggcgaaaacgctgggcgtttccgaagcttggctgatgggatttgatgttccaatggttgaatcgtcgaaaatcgaaaacgattcggaaaacatcgaagaaaccattacggtgatgaaaaaattggaagagccgagacagaaagtggtcctggataccgccaaaattcagctgaaagaacaggatgagcagaacaaagtgaaacagatcgaagattaccgtctaagtgatgaatatttagaagaacagatcagtaaagcgagtgcatatggaggtggccaattaaatgacaacgataaagaatttttcaaacgtttgttgaaaaacacgttaaaagaaaaaattgataaaggagatctggctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagcgcatcttgaacaaaagttcaacaaaaaaattcatatttcgtgaacttttttgttgacaaagataaaaacacatgatatactaatttcataaagttcatgaaacgtgaactgaaatt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J06803_sequence/1
type: Sequence
persistent identity: BBa J06803 sequence
display id: BBa_J06803_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatgagcacaaaaaagaaaccattaacacaagagcagcttgaggacgcacgtcgccttaaagcaatttatgaaaaaaagaaaaatgaacttggcttatcccaggaatctgtcgcagacaagatggggatggggcagtcaggcgttggtgctttatttaatggcatcaatgcattaaatgcttataacgccgcattgcttacaaaaattctcaaagttagcgttgaagaatttagcccttcaatcgccagagaaatctacgagatgtatgaagcggttagtatgcagccgtcacttagaagtgagtatgagtaccctgttttttctcatgttcaggcagggatgttctcacctaagcttagaacctttaccaaaggtgatgcggagagatgggtaagcacaaccaaaaaagccagtgattctgcattctggcttgaggttgaaggtaattccatgaccgcaccaacaggctccaagccaagctttcctgacggaatgttaattctcgttgaccctgagcaggctgttgagccaggtgatttctgcatagccagacttgggggtgatgagtttaccttcaagaaactgatcagggatagcggtcaggtgtttttacaaccactaaacccacagtacccaatgatcccatgcaatgagagttgttccgttgtggggaaagttatcgctagtcagtggcctgaagagacgtttggcgctgcaaacgacgaaaactacgctttagtagcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtaacaccgtgcgtgttgactattttacctctggcggtgataatggttgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I9201_sequence/1
type: Sequence
persistent identity: BBa I9201 sequence
display id: BBa_I9201_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tgcaatttagatagtggcgttccctatttatagattgtggcagcgacaactgataaaatggagaccgccactattaccaacg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0053_sequence/1
type: Sequence
persistent identity: BBa C0053 sequence
display id: BBa_C0053_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaatacacaattgatgggtgagcgtattcgcgctcgaagaaaaaaactcaagattagacaagccgctcttggtaagatggtgggagtgtctaatgttgcaatatcgcaatgggagcgctcggagactgagccaaatggggagaacctgttggcactttcgaaggctcttcagtgctcccctgactatttgctgaaaggagatttaagccagacaaacgttgcctatcatagtaggcatgagccaagaggatcataccctcttatcagttgggtaagcgcagggcaatggatggaagctgtagaaccttatcacaagcgcgcgatagagaactggcacgacaccactgtagattgttcagaagattcattttggcttgatgtccaaggtgactctatgacagcaccggcagggttaagcattccagaaggaatgataattctggttgatcccgaagtcgaaccaagaaacggcaagctggttgttgcaaaattagaaggtgaaaacgaggccacattcaaaaaattagttatggatgcaggccgaaagtttttaaaaccattaaacccacaatatccgatgatagaaatcaacggaaactgcaaaatcattggcgtagttgttgacgcaaaactcgcaaatcttccagctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0031_sequence/1
type: Sequence
persistent identity: BBa B0031 sequence
display id: BBa_B0031_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacacaggaaacc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I9001_sequence/1
type: Sequence
persistent identity: BBa I9001 sequence
display id: BBa_I9001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatggcccgggatgatcctcacttcaattttaaaatgccaatggaagtaagagagaaattgaaatttagagcagaggcaaacggacggagcatgaactctgagcttttgcaaatcgtacaagatgccctaagcaaaccgtcaccagtcactgggtaccgcaatgatgcggaacgactcgccgatgagcagagcgagttagtgaagaagatggtcttcgatacactgaaggatctttataaaaaaaccaccgctgcaaacgacgaaaactacgctttagtagcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagctcgaggtgagtgcacagtactaggtccacggtgacctagatctcctatagtgagtcgtattaattt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0062_sequence/1
type: Sequence
persistent identity: BBa C0062 sequence
display id: BBa_C0062_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0062_sequence/1
type: Sequence
persistent identity: BBa R0062 sequence
display id: BBa_R0062_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: acctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_Q08400_sequence/1
type: Sequence
persistent identity: BBa Q08400 sequence
display id: BBa_Q08400_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacacaggaaaccggttcgatgtactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0038_sequence/1
type: Sequence
persistent identity: BBa B0038 sequence
display id: BBa_B0038_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacacaggaaaccggttcgatg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J06500_sequence/1
type: Sequence
persistent identity: BBa J06500 sequence
display id: BBa_J06500_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactacgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcaggctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0065_sequence/1
type: Sequence
persistent identity: BBa R0065 sequence
display id: BBa_R0065_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: taagcacctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataacaccgtgcgtgttgactattttacctctggcggtgata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I13409_sequence/1
type: Sequence
persistent identity: BBa I13409 sequence
display id: BBa_I13409_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cgcgccaagcttaaagaggagaaatactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcacccatggcagtcg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0051_sequence/1
type: Sequence
persistent identity: BBa C0051 sequence
display id: BBa_C0051_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgagcacaaaaaagaaaccattaacacaagagcagcttgaggacgcacgtcgccttaaagcaatttatgaaaaaaagaaaaatgaacttggcttatcccaggaatctgtcgcagacaagatggggatggggcagtcaggcgttggtgctttatttaatggcatcaatgcattaaatgcttataacgccgcattgcttgcaaaaattctcaaagttagcgttgaagaatttagcccttcaatcgccagagaaatctacgagatgtatgaagcggttagtatgcagccgtcacttagaagtgagtatgagtaccctgttttttctcatgttcaggcagggatgttctcacctgagcttagaacctttaccaaaggtgatgcggagagatgggtaagcacaaccaaaaaagccagtgattctgcattctggcttgaggttgaaggtaattccatgaccgcaccaacaggctccaagccaagctttcctgacggaatgttaattctcgttgaccctgagcaggctgttgagccaggtgatttctgcatagccagacttgggggtgatgagtttaccttcaagaaactgatcagggatagcggtcaggtgtttttacaaccactaaacccacagtacccaatgatcccatgcaatgagagttgttccgttgtggggaaagttatcgctagtcagtggcctgaagagacgtttggcgctgcaaacgacgaaaactacgctttagtagcttaataacgctgatagtgctagtgtagatcgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0012_sequence/1
type: Sequence
persistent identity: BBa C0012 sequence
display id: BBa_C0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcaggctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_Q04400_sequence/1
type: Sequence
persistent identity: BBa Q04400 sequence
display id: BBa_Q04400_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J06801_sequence/1
type: Sequence
persistent identity: BBa J06801 sequence
display id: BBa_J06801_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttgatgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcaggctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagaattgtgagcggataacaattgacattgtgagcggataacaagatactgagcaca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0011_sequence/1
type: Sequence
persistent identity: BBa R0011 sequence
display id: BBa_R0011_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcaca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_Q00400_sequence/1
type: Sequence
persistent identity: BBa Q00400 sequence
display id: BBa_Q00400_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: attaaagaggagaaatactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0041_sequence/1
type: Sequence
persistent identity: BBa B0041 sequence
display id: BBa_B0041_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacacaggacggccggatg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I9002_sequence/1
type: Sequence
persistent identity: BBa I9002 sequence
display id: BBa_I9002_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatgaaaaaaataccacagatttctgatgcggaactcgaagtcatgaaagtgatttggaagcattcttccattaatactaatgaggtaatcaaagagctcagtaaaacttcaacgtggagcccaaaaactattcagactatgctgctgcgccttatcaaaaaaggcgctctcaaccaccataaagaaggtcgggttttcgtttacacgcctaatatagacgaatcagattatatagaggttaagtcacactcatttctcaaccggttttacaatggtacattaaattccatggtactcaactttttggagaatgatcaactgtcgggagaagaaatcaatgaattgtatcagatactcgaagaacataagaaccgtaagaaggaagctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtcatttccaaccgaaaaaaggcttgcatttaaatcttacatatgtaatactttcaaagactacatttgtaagatttg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0052_sequence/1
type: Sequence
persistent identity: BBa R0052 sequence
display id: BBa_R0052_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgacaaacaagatacattgtatgaaaatacaagaaagtttgttga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0064_sequence/1
type: Sequence
persistent identity: BBa B0064 sequence
display id: BBa_B0064_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggggaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0075_sequence/1
type: Sequence
persistent identity: BBa C0075 sequence
display id: BBa_C0075_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaaaaccgataccagtaacagattaaaacaaataatggcggaacgcaacctgaaacaggtggatatcttaaacttatcgattccatttcagaaaaaatttggtatcaagctgagtaaaagtacgctgtcgcagtatgtgaactctgtgcagtcgccagaccagaaccgcatctatttactggcgaaaacgctgggcgtttccgaagcttggctgatgggatttgatgttccaatggttgaatcgtcgaaaatcgaaaacgattcggaaaacatcgaagaaaccattacggtgatgaaaaaattggaagagccgagacagaaagtggtcctggataccgccaaaattcagctgaaagaacaggatgagcagaacaaagtgaaacagatcgaagattaccgtctaagtgatgaatatttagaagaacagatcagtaaagcgagtgcatatggaggtggccaattaaatgacaacgataaagaatttttcaaacgtttgttgaaaaacacgttaaaagaaaaaattgataaaggagatctggctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0074_sequence/1
type: Sequence
persistent identity: BBa C0074 sequence
display id: BBa_C0074_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaaaaaaataccacagatttctgatgcggaactcgaagtcatgaaagtgatttggaagcattcttccattaatactaatgaggtaatcaaagagctcagtaaaacttcaacgtggagcccaaaaactattcagactatgctgctgcgccttatcaaaaaaggcgctctcaaccaccataaagaaggtcgggttttcgtttacacgcctaatatagacgaatcagattatatagaggttaagtcacactcatttctcaaccggttttacaatggtacattaaattccatggtactcaactttttggagaatgatcaactgtcgggagaagaaatcaatgaattgtatcagatactcgaagaacataagaaccgtaagaaggaagctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_Q01122_sequence/1
type: Sequence
persistent identity: BBa Q01122 sequence
display id: BBa_Q01122_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacacaggaaacctactagatggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcaggctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagcaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0052_sequence/1
type: Sequence
persistent identity: BBa C0052 sequence
display id: BBa_C0052_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgagtatttcttccagggtaaaaagcaaaagaatccagcttggacttaaccaggctgaacttgctcaaaaggtggggactacccagcagtctatagagcagctcgaaaacggtaaaactaagcgaccacgctttttaccagaacttgcgtcagctcttggcgtaagtgttgactggctgctcaatggcacctctgattcgaatgttagatttgttgggcacgttgagcccaaagggaaatatccattgattagcatggttagagctggttcgtggtgtgaagcttgtgaaccctacgatatcaaggacattgatgaatggtatgacagtgacgttaacttattaggcaatggattctggctgaaggttgaaggtgattccatgacctcacctgtaggtcaaagcatccctgaaggtcatatggtgttagtagatactggacgggagccagtgaatggaagccttgttgtagccaaactgactgacgcgaacgaagcaacattcaagaaactggtcatagatggcggtcagaagtacctgaaaggcctgaatccttcatggcctatgactcctatcaacggaaactgcaagattatcggtgttgtcgtggaagcgagggtaaaattcgtagctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0039_sequence/1
type: Sequence
persistent identity: BBa B0039 sequence
display id: BBa_B0039_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacacaggaaaggcctcgatg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I9200_sequence/1
type: Sequence
persistent identity: BBa I9200 sequence
display id: BBa_I9200_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgagcactaaaaagaaaccacttactcaagagcagttagaggacgcccgtcgcttaaaagccatttatgaaaaaaagaaaaatgaattaggcctttcccaggaatcagtcgccgacaagatggggatggggcagtcgggcgttggtgctctttttaatggcatcaatgcccttaatgcttataacgcggccctcttagccaaaattttgaaagttagcgttgaagaatttagcccttcgatcgcgcgagaaatctacgagatgtatgaagcagttagtatgcagccgtcgttacgaagtgagtatgagtaccctgttttttcacatgttcaggccgggatgttctcgcctgagttacgaacctttaccaaaggtgatgcagagcgatgggtaagcactaccaaaaaagcgagtgattcagccttctggttagaggttgaaggtaattccatgaccgccccaactggctccaagccaagctttcctgacggaatgcttattttggttgaccctgagcaggctgttgagccaggtgatttctgcatagcgcgattagggggtgatgagtttaccttcaagaaactgatcagggatagcggtcaggtgtttcttcaaccactaaacccacagtacccaatgatcccatgcaatgagagttgttccgttgtggggaaagttatcgctagtcagtggcctgaagagacgtttggctaataactctgatagtgctagtgtagatctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_A340620_sequence/1
type: Sequence
persistent identity: BBa A340620 sequence
display id: BBa_A340620_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagacctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J06501_sequence/1
type: Sequence
persistent identity: BBa J06501 sequence
display id: BBa_J06501_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttgatgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcaggctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_Q04530_sequence/1
type: Sequence
persistent identity: BBa Q04530 sequence
display id: BBa_Q04530_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatgaatacacaattgatgggtgagcgtattcgcgctcgaagaaaaaaactcaagattagacaagccgctcttggtaagatggtgggagtgtctaatgttgcaatatcgcaatgggagcgctcggagactgagccaaatggggagaacctgttggcactttcgaaggctcttcagtgctcccctgactatttgctgaaaggagatttaagccagacaaacgttgcctatcatagtaggcatgagccaagaggatcataccctcttatcagttgggtaagcgcagggcaatggatggaagctgtagaaccttatcacaagcgcgcgatagagaactggcacgacaccactgtagattgttcagaagattcattttggcttgatgtccaaggtgactctatgacagcaccggcagggttaagcattccagaaggaatgataattctggttgatcccgaagtcgaaccaagaaacggcaagctggttgttgcaaaattagaaggtgaaaacgaggccacattcaaaaaattagttatggatgcaggccgaaagtttttaaaaccattaaacccacaatatccgatgatagaaatcaacggaaactgcaaaatcattggcgtagttgttgacgcaaaactcgcaaatcttccagctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagaataaacttgactaaagattcctttagtagataatttaagtgttctttaatttc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_Q00121_sequence/1
type: Sequence
persistent identity: BBa Q00121 sequence
display id: BBa_Q00121_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: attaaagaggagaaatactagatggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcaggctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagaattgtgagcggataacaattgacattgtgagcggataacaagatactgagcaca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0032_sequence/1
type: Sequence
persistent identity: BBa B0032 sequence
display id: BBa_B0032_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacacaggaaag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0053_sequence/1
type: Sequence
persistent identity: BBa R0053 sequence
display id: BBa_R0053_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aataaacttgactaaagattcctttagtagataatttaagtgttctttaatttc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0075_sequence/1
type: Sequence
persistent identity: BBa R0075 sequence
display id: BBa_R0075_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cgcatcttgaacaaaagttcaacaaaaaaattcatatttcgtgaacttttttgttgacaaagataaaaacacatgatatactaatttcataaagttcatgaaacgtgaactgaaatt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_Q04520_sequence/1
type: Sequence
persistent identity: BBa Q04520 sequence
display id: BBa_Q04520_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatgagtatttcttccagggtaaaaagcaaaagaatccagcttggacttaaccaggctgaacttgctcaaaaggtggggactacccagcagtctatagagcagctcgaaaacggtaaaactaagcgaccacgctttttaccagaacttgcgtcagctcttggcgtaagtgttgactggctgctcaatggcacctctgattcgaatgttagatttgttgggcacgttgagcccaaagggaaatatccattgattagcatggttagagctggttcgtggtgtgaagcttgtgaaccctacgatatcaaggacattgatgaatggtatgacagtgacgttaacttattaggcaatggattctggctgaaggttgaaggtgattccatgacctcacctgtaggtcaaagcatccctgaaggtcatatggtgttagtagatactggacgggagccagtgaatggaagccttgttgtagccaaactgactgacgcgaacgaagcaacattcaagaaactggtcatagatggcggtcagaagtacctgaaaggcctgaatccttcatggcctatgactcctatcaacggaaactgcaagattatcggtgttgtcgtggaagcgagggtaaaattcgtagctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagttgacaaacaagatacattgtatgaaaatacaagaaagtttgttga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_Q02400_sequence/1
type: Sequence
persistent identity: BBa Q02400 sequence
display id: BBa_Q02400_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacacaggaaagtactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_Q04511_sequence/1
type: Sequence
persistent identity: BBa Q04511 sequence
display id: BBa_Q04511_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatgagcacaaaaaagaaaccattaacacaagagcagcttgaggacgcacgtcgccttaaagcaatttatgaaaaaaagaaaaatgaacttggcttatcccaggaatctgtcgcagacaagatggggatggggcagtcaggcgttggtgctttatttaatggcatcaatgcattaaatgcttataacgccgcattgcttgcaaaaattctcaaagttagcgttgaagaatttagcccttcaatcgccagagaaatctacgagatgtatgaagcggttagtatgcagccgtcacttagaagtgagtatgagtaccctgttttttctcatgttcaggcagggatgttctcacctgagcttagaacctttaccaaaggtgatgcggagagatgggtaagcacaaccaaaaaagccagtgattctgcattctggcttgaggttgaaggtaattccatgaccgcaccaacaggctccaagccaagctttcctgacggaatgttaattctcgttgaccctgagcaggctgttgagccaggtgatttctgcatagccagacttgggggtgatgagtttaccttcaagaaactgatcagggatagcggtcaggtgtttttacaaccactaaacccacagtacccaatgatcccatgcaatgagagttgttccgttgtggggaaagttatcgctagtcagtggcctgaagagacgtttggcgctgcaaacgacgaaaactacgctttagtagcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtaagcacctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataacaccgtgcgtgttgactattttacctctggcggtgata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_Q07400_sequence/1
type: Sequence
persistent identity: BBa Q07400 sequence
display id: BBa_Q07400_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacacaggacggccggatgtactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J06502_sequence/1
type: Sequence
persistent identity: BBa J06502 sequence
display id: BBa_J06502_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgagcacaaaaaagaaaccattaacacaagagcagcttgaggacgcacgtcgccttaaagcaatttatgaaaaaaagaaaaatgaacttggcttatcccaggaatctgtcgcagacaagatggggatggggcagtcaggcgttggtgctttatttaatggcatcaatgcattaaatgcttataacgccgcattgcttgcaaaaattctcaaagttagcgttgaagaatttagcccttcaatcgccacagaaatctacgagatgtatgaagcggttagtatgcagccgtcacttagaagtgagtatgagtaccctgttttttctcatgttcaggcagggatgttctcacctaagcttagaacctttaccaaaggtgatgcggagagatgggtaagcacaaccaaaaaagccagtgattctgcattctggcttgaggttgaaggtaattccatgaccgcaccaacaggctccaagccaagctttcctgacggaatgttaattctcgttgaccctgagcaggctgttgagccaggtgatttctgcatagccagacttgggggtgatgagtttaccttcaagaaactgatcagggatagcggtcaggtgtttttacaaccactaaacccacagtacccaatgatcccatgcaatgagagttgttccgttgtggggaaagttatggctagtcagtggcctgaagagacgtttggcgctgcaaacgacgaaaactacgctttagtagcttaataacgctgatagtgctagtgtagatcgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J06802_sequence/1
type: Sequence
persistent identity: BBa J06802 sequence
display id: BBa_J06802_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatgagcacaaaaaagaaaccattaacacaagagcagcttgaggacgcacgtcgccttaaagcaatttatgaaaaaaagaaaaatgaacttggcttatcccaggaatctgtcgcagacaagatggggatggggcagtcaggcgttggtgctttatttaatggcatcaatgcattaaatgcttataacgccgcattgcttgcaaaaattctcaaagttagcgttgaagaatttagcccttcaatcgccacagaaatctacgagatgtatgaagcggttagtatgcagccgtcacttagaagtgagtatgagtaccctgttttttctcatgttcaggcagggatgttctcacctaagcttagaacctttaccaaaggtgatgcggagagatgggtaagcacaaccaaaaaagccagtgattctgcattctggcttgaggttgaaggtaattccatgaccgcaccaacaggctccaagccaagctttcctgacggaatgttaattctcgttgaccctgagcaggctgttgagccaggtgatttctgcatagccagacttgggggtgatgagtttaccttcaagaaactgatcagggatagcggtcaggtgtttttacaaccactaaacccacagtacccaatgatcccatgcaatgagagttgttccgttgtggggaaagttatggctagtcagtggcctgaagagacgtttggcgctgcaaacgacgaaaactacgctttagtagcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtaacaccgtgcgtgttgactattttacctctggcggtgataatggttgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0040_sequence/1
type: Sequence
persistent identity: BBa R0040 sequence
display id: BBa_R0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0030_sequence/1
type: Sequence
persistent identity: BBa B0030 sequence
display id: BBa_B0030_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: attaaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0040_sequence/1
type: Sequence
persistent identity: BBa C0040 sequence
display id: BBa_C0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0015_sequence/1
type: Sequence
persistent identity: BBa B0015 sequence
display id: BBa_B0015_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_Q02121_sequence/1
type: Sequence
persistent identity: BBa Q02121 sequence
display id: BBa_Q02121_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacacaggaaagtactagatggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcaggctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagaattgtgagcggataacaattgacattgtgagcggataacaagatactgagcaca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I9004_sequence/1
type: Sequence
persistent identity: BBa I9004 sequence
display id: BBa_I9004_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatgagcactaaaaagaaaccacttactcaagagcagttagaggacgcccgtcgcttaaaagccatttatgaaaaaaagaaaaatgaattaggcctttcccaggaatcagtcgccgacaagatggggatggggcagtcgggcgttggtgctctttttaatggcatcaatgcccttaatgcttataacgcggccctcttagccaaaattttgaaagttagcgttgaagaatttagcccttcgatcgcgcgagaaatctacgagatgtatgaagcagttagtatgcagccgtcgttacgaagtgagtatgagtaccctgttttttcacatgttcaggccgggatgttctcgcctgagttacgaacctttaccaaaggtgatgcagagcgatgggtaagcactaccaaaaaagcgagtgattcagccttctggttagaggttgaaggtaattccatgaccgccccaactggctccaagccaagctttcctgacggaatgcttattttggttgaccctgagcaggctgttgagccaggtgatttctgcatagcgcgattagggggtgatgagtttaccttcaagaaactgatcagggatagcggtcaggtgtttcttcaaccactaaacccacagtacccaatgatcccatgcaatgagagttgttccgttgtggggaaagttatcgctagtcagtggcctgaagagacgtttggctaataactctgatagtgctagtgtagatctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtgcaatttagatagtggcgttccctatttatagattgtggcagcgacaactgataaaatggagaccgccactattaccaacg
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z