Tet QPI with B0032 which according to preliminary experiments is a "medium" strength RBS.
First attempt to mutate failed. Suspect the problem is a low mutation rate due to results of positive control. May be a result of the mutagenesis kit being old. Second attempt to mutate failed. Sent out to Blue Heron for synthesis.
First attempt to mutate failed. Suspect the problem is a low mutation rate due to results of positive control. May be a result of the mutagenesis kit being old. Second attempt to mutate failed. Abandoned,
Quadpart Amplifier (B0034.C0062.B0015.R0062)
Currently entered as a placeholder. This is a modified version of the tetR inverter Q04400 with a single base switch in the RBS: AAAGAGGAGAAA to AAAGAGGGGAAA
Tet QPI with B0030 which according to preliminary experiments is a "strong" strength RBS.
Mutation has been sequenced. The rest of the part will be sequenced shortly.
HindIII - Q04400 - NcoI Note that this part does NOT have the standard biobrick ends and is available only as a PCR product.
LacI QPI with strong RBS, hybrid promoter
LacI QPI with B0032 which according to preliminary experiments is a "medium" strength RBS. Also uses hybrid promoter.
Lambda cI QPI w/ strong RBS, hybrid promoter regulated by lambda cI and LuxR
LacI QPI with B0030 which according to preliminary experiments is a "medium" strength RBS. Also uses hybrid promoter.
This biobrick contains the sequence homologies (50bp) to delete lpp gen (LPS moiety carrier) in E. coli with an ampicillin resistance and a Multiple Cloning Site (for the generation of genomic knock-ins of any desired gen in E. coli). The deletion of lpp gene and the replacement of that sequence with the Amp resistance. Gene deletions are performed with lambda-red recombination technology. FLP-FRT sequences are included to remove the antibiotic resistance with a specific recombinase.
This is the general structure 50bp homology Lpp // FRT // Amp R Promoter // RBS // AmpR // Terminator // FRT // MCS // 50 bp homology Lpp
The genetic design is based in the lpp deletion done by: Ni, Y., Reyes, J., & Chen, R. (2007). lpp Deletion as a Permeabilization Method. Biotechnology and Bioengineering, 1347-1356. It also contains some parts of the registry like BBa_K1073000, BBa_B0034, BBa_B0015