//classic/measurement/uncategorized
type: Collection
persistent identity: classic measurement uncategorized
display id: classic_measurement_uncategorized
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
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owned by: myers
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BBa_I6004
type: ComponentDefinition
persistent identity: BBa I6004
display id: BBa_I6004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: QPI Curve Test I0500.E0422.I0500.Q04122.E0432
creator: Caitlin Conboy, Jennifer Braff
created: 2003-12-05T12:00:00Z
modified: 2015-08-31T04:07:42Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: QPI characterization assembly
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Measurement
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component: BBa_E0022
component: BBa_I0500
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component: BBa_B0012
component: BBa_R0010
component: BBa_B0034
component: BBa_C0012
sequence annotation: BBa_B0010
sequence annotation: BBa_I0500
sequence annotation: BBa_B0034
sequence annotation: BBa_R0010
sequence annotation: BBa_B0012
sequence annotation: BBa_E0022
sequence annotation: BBa_C0012
sequence annotation: BBa_E0032
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BBa_B0010
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BBa_B0034
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BBa_B0010
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BBa_M30009
type: ComponentDefinition
persistent identity: BBa M30009
display id: BBa_M30009
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: -- No description --
creator: natalie kuldell
created: 2005-12-04T12:00:00Z
modified: 2015-05-08T01:13:59Z
discontinued: false
dominant: true
group u list: _45_1_
m user id: 0
owner id: 314
owning group id: 1
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Positively regulated, OmpR-controlled promoter from OmpC gene (R0082) directing transcription of lambda cI gene. The cI protein represses expression of mRFP (M30003). Constructed with standard assembly method except R0082 was isolated using VF and VR-directed PCR. Consequently, there may be silent mutations that were inadvertently introduced.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Measurement
component: BBa_C0051
component: BBa_R0051
component: BBa_B0034
component: BBa_R0082
component: BBa_B0010
component: BBa_B0012
component: BBa_E1010
sequence annotation: BBa_E1010
sequence annotation: BBa_C0051
sequence annotation: BBa_R0082
sequence annotation: BBa_B0010
sequence annotation: BBa_B0034
sequence annotation: BBa_B0012
sequence annotation: BBa_R0051
sequence: https://synbiohub.org/public/igem/BBa_M30009_sequence/1

BBa_C0051
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BBa_R0051
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BBa_B0034
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BBa_R0082
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BBa_B0010
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BBa_B0012
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BBa_B0010
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BBa_B0012
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BBa_B0034
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BBa_E1010
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BBa_E1010
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BBa_R0082
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BBa_B0010
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BBa_B0010
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description: Cascaded Tet (elowitz) and Lac (medium) QPIs
creator: mit
created: 2004-07-19T11:00:00Z
modified: 2015-08-31T04:07:36Z
discontinued: false
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was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Cascaded Lac (medium) and Tet (elowitz) QPIs with double terminator as input
creator: mit
created: 2004-07-19T11:00:00Z
modified: 2015-08-31T04:07:36Z
discontinued: false
dominant: false
experience: experience/None
group u list: _6_
m user id: 0
owner id: 101
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: false
type: DnaRegion
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component: BBa_B0015
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persistent identity: BBa M30008
display id: BBa_M30008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: -- No description --
creator: natalie kuldell
created: 2005-12-04T12:00:00Z
modified: 2015-05-08T01:13:59Z
discontinued: false
dominant: true
group u list: _45_1_
m user id: 0
owner id: 314
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sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Positively regulated, OmpR-controlled promoter from OmpC gene (R0082) directing transcription of P22 c2 gene. The P22 c2 protein represses expression of mRFP (M30002). Constructed with standard assembly method except R0082 was isolated using VF and VR-directed PCR. Consequently, there may be silent mutations that were inadvertently introduced. A second isolate from this construction was saved as M30007.
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star: false
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BBa_I6016
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persistent identity: BBa I6016
display id: BBa_I6016
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: QPI Curve Test I0500.E0422.I0500.Q04511.E0432
creator: Caitlin Conboy, Jennifer Braff
created: 2003-12-05T12:00:00Z
modified: 2015-08-31T04:07:43Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
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owning group id: 7
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: QPI characterization assembly
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star: true
type: DnaRegion
role: SO:0000804
role: partType/Measurement
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component: BBa_B0012
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end: 1210
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BBa_B0010
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location: https://synbiohub.org/public/igem/BBa_I6016/annotation979424/range979424/1
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BBa_B0010
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BBa_B0010
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BBa_B0012
type: SequenceAnnotation
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version: 1
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BBa_B0034
type: SequenceAnnotation
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BBa_C0051
type: SequenceAnnotation
persistent identity: annotation979454
display id: annotation979454
version: 1
top level: BBa_I6016
location: https://synbiohub.org/public/igem/BBa_I6016/annotation979454/range979454/1
component: BBa_C0051

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display id: range979454
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start: 3380
end: 4129
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BBa_K1682009
type: ComponentDefinition
persistent identity: BBa K1682009
display id: BBa_K1682009
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PkdpF[-15, T>G] - GFP generator
creator: iGEM15_HKUST-Rice
created: 2015-08-10T11:00:00Z
modified: 2015-09-18T09:11:00Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2100_
m user id: 8758
owner id: 21235
owning group id: 9
status: status/Planning
bookmark: false
mutable description: kdpFp[-15, T>G] - E0240
mutable notes: kdpFp[-15, T>G] - E0240
mutable provenance: kdpFp[-15, T>G] - E0240
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Measurement
component: BBa_K1682004
component: BBa_E0240
sequence annotation: BBa_E0240
sequence annotation: BBa_K1682004
sequence: https://synbiohub.org/public/igem/BBa_K1682009_sequence/1

BBa_K1682004
type: Component
persistent identity: component2434955
display id: component2434955
version: 1
top level: BBa_K1682009
access: public
definition: https://synbiohub.org/public/igem/BBa_K1682004/1

BBa_E0240
type: Component
persistent identity: component2434966
display id: component2434966
version: 1
top level: BBa_K1682009
access: public
definition: https://synbiohub.org/public/igem/BBa_E0240/1

BBa_E0240
type: SequenceAnnotation
persistent identity: annotation2434966
display id: annotation2434966
version: 1
top level: BBa_K1682009
location: https://synbiohub.org/public/igem/BBa_K1682009/annotation2434966/range2434966/1
component: BBa_E0240

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BBa_K1682004
type: SequenceAnnotation
persistent identity: annotation2434955
display id: annotation2434955
version: 1
top level: BBa_K1682009
location: https://synbiohub.org/public/igem/BBa_K1682009/annotation2434955/range2434955/1
component: BBa_K1682004

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persistent identity: range2434955
display id: range2434955
version: 1
direction: direction/reverse
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BBa_I7023
type: ComponentDefinition
persistent identity: BBa I7023
display id: BBa_I7023
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: R0010.E0021.B0023.E0031
creator: Caitlin Conboy
created: 2004-04-22T11:00:00Z
modified: 2015-08-31T04:07:45Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Terminator Characterization Construct
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Measurement
component: BBa_E0031
component: BBa_E0021
component: BBa_R0010
component: BBa_B0023
sequence annotation: BBa_R0010
sequence annotation: BBa_E0021
sequence annotation: BBa_E0031
sequence annotation: BBa_B0023
sequence: https://synbiohub.org/public/igem/BBa_I7023_sequence/1

BBa_E0031
type: Component
persistent identity: component944160
display id: component944160
version: 1
top level: BBa_I7023
access: public
definition: https://synbiohub.org/public/igem/BBa_E0031/1

BBa_E0021
type: Component
persistent identity: component944147
display id: component944147
version: 1
top level: BBa_I7023
access: public
definition: https://synbiohub.org/public/igem/BBa_E0021/1

BBa_R0010
type: Component
persistent identity: component944131
display id: component944131
version: 1
top level: BBa_I7023
access: public
definition: https://synbiohub.org/public/igem/BBa_R0010/1

BBa_B0023
type: Component
persistent identity: component944149
display id: component944149
version: 1
top level: BBa_I7023
access: public
definition: https://synbiohub.org/public/igem/BBa_B0023/1

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation944131
display id: annotation944131
version: 1
top level: BBa_I7023
location: https://synbiohub.org/public/igem/BBa_I7023/annotation944131/range944131/1
component: BBa_R0010

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display id: range944131
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type: SequenceAnnotation
persistent identity: annotation944147
display id: annotation944147
version: 1
top level: BBa_I7023
location: https://synbiohub.org/public/igem/BBa_I7023/annotation944147/range944147/1
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end: 948
orientation: inline

BBa_E0031
type: SequenceAnnotation
persistent identity: annotation944160
display id: annotation944160
version: 1
top level: BBa_I7023
location: https://synbiohub.org/public/igem/BBa_I7023/annotation944160/range944160/1
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persistent identity: range944160
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version: 1
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end: 1751
orientation: inline

BBa_B0023
type: SequenceAnnotation
persistent identity: annotation944149
display id: annotation944149
version: 1
top level: BBa_I7023
location: https://synbiohub.org/public/igem/BBa_I7023/annotation944149/range944149/1
component: BBa_B0023

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type: Range
persistent identity: range944149
display id: range944149
version: 1
direction: direction/forward
top level: BBa_I7023
start: 957
end: 1003
orientation: inline

BBa_I6301
type: ComponentDefinition
persistent identity: BBa I6301
display id: BBa_I6301
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: QPI Curve Test I0500.E0420.I0500.Q04121.E0430
creator: cconboy
created: 2004-05-09T11:00:00Z
modified: 2015-08-31T04:07:44Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_1_
m user id: 0
owner id: 60
owning group id: 7
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000804
role: partType/Measurement
component: BBa_I0500
component: BBa_E0420
component: BBa_E0430
component: BBa_Q04121
sequence annotation: BBa_I0500
sequence annotation: BBa_E0430
sequence annotation: BBa_Q04121
sequence annotation: BBa_E0420
sequence: https://synbiohub.org/public/igem/BBa_I6301_sequence/1

BBa_I0500
type: Component
persistent identity: component2234671
display id: component2234671
version: 1
top level: BBa_I6301
access: public
definition: https://synbiohub.org/public/igem/BBa_I0500/1

BBa_E0420
type: Component
persistent identity: component2234667
display id: component2234667
version: 1
top level: BBa_I6301
access: public
definition: https://synbiohub.org/public/igem/BBa_E0420/1

BBa_E0430
type: Component
persistent identity: component2234700
display id: component2234700
version: 1
top level: BBa_I6301
access: public
definition: https://synbiohub.org/public/igem/BBa_E0430/1

BBa_Q04121
type: Component
persistent identity: component2234690
display id: component2234690
version: 1
top level: BBa_I6301
access: public
definition: https://synbiohub.org/public/igem/BBa_Q04121/1

BBa_I0500
type: Component
persistent identity: component2234657
display id: component2234657
version: 1
top level: BBa_I6301
access: public
definition: https://synbiohub.org/public/igem/BBa_I0500/1

BBa_I0500
type: SequenceAnnotation
persistent identity: annotation2234671
display id: annotation2234671
version: 1
top level: BBa_I6301
location: https://synbiohub.org/public/igem/BBa_I6301/annotation2234671/range2234671/1
component: BBa_I0500

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display id: range2234671
version: 1
direction: direction/reverse
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start: 2105
end: 3314
orientation: inline

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type: SequenceAnnotation
persistent identity: annotation2234657
display id: annotation2234657
version: 1
top level: BBa_I6301
location: https://synbiohub.org/public/igem/BBa_I6301/annotation2234657/range2234657/1
component: BBa_I0500

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persistent identity: range2234657
display id: range2234657
version: 1
direction: direction/reverse
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start: 1
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orientation: inline

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type: SequenceAnnotation
persistent identity: annotation2234700
display id: annotation2234700
version: 1
top level: BBa_I6301
location: https://synbiohub.org/public/igem/BBa_I6301/annotation2234700/range2234700/1
component: BBa_E0430

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persistent identity: range2234700
display id: range2234700
version: 1
direction: direction/reverse
top level: BBa_I6301
start: 4702
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type: SequenceAnnotation
persistent identity: annotation2234690
display id: annotation2234690
version: 1
top level: BBa_I6301
location: https://synbiohub.org/public/igem/BBa_I6301/annotation2234690/range2234690/1
component: BBa_Q04121

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persistent identity: range2234690
display id: range2234690
version: 1
direction: direction/reverse
top level: BBa_I6301
start: 3323
end: 4693
orientation: inline

BBa_E0420
type: SequenceAnnotation
persistent identity: annotation2234667
display id: annotation2234667
version: 1
top level: BBa_I6301
location: https://synbiohub.org/public/igem/BBa_I6301/annotation2234667/range2234667/1
component: BBa_E0420

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type: Range
persistent identity: range2234667
display id: range2234667
version: 1
direction: direction/reverse
top level: BBa_I6301
start: 1219
end: 2096
orientation: inline

BBa_K772103
type: ComponentDefinition
persistent identity: BBa K772103
display id: BBa_K772103
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: GFP - LacZ protein complex generator
creator: Mustafa Elitok
created: 2012-09-25T11:00:00Z
modified: 2015-05-08T01:13:15Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1024_
m user id: 0
owner id: 9573
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: GFP - LacZ protein generator is generated with the aim of inducing a reporting reaction aftermath of the activation of EpCAM detection system. (BBa_K772100) For more information: BBa_K772006
mutable notes: To achieve a constitutive production, pTetR promoter is added in the upstream region of the original part.
mutable provenance: Cleavage site, LacZ and GFP sequences are gathered from Registry.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_R0040
component: BBa_K772006
sequence annotation: BBa_R0040
sequence annotation: BBa_K772006
sequence: https://synbiohub.org/public/igem/BBa_K772103_sequence/1

BBa_R0040
type: Component
persistent identity: component2211349
display id: component2211349
version: 1
top level: BBa_K772103
access: public
definition: https://synbiohub.org/public/igem/BBa_R0040/1

BBa_K772006
type: Component
persistent identity: component2211360
display id: component2211360
version: 1
top level: BBa_K772103
access: public
definition: AnRe

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation2211349
display id: annotation2211349
version: 1
top level: BBa_K772103
location: https://synbiohub.org/public/igem/BBa_K772103/annotation2211349/range2211349/1
component: BBa_R0040

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type: Range
persistent identity: range2211349
display id: range2211349
version: 1
direction: direction/forward
top level: BBa_K772103
start: 1
end: 54
orientation: inline

BBa_K772006
type: SequenceAnnotation
persistent identity: annotation2211360
display id: annotation2211360
version: 1
top level: BBa_K772103
location: https://synbiohub.org/public/igem/BBa_K772103/annotation2211360/range2211360/1
component: BBa_K772006

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type: Range
persistent identity: range2211360
display id: range2211360
version: 1
direction: direction/reverse
top level: BBa_K772103
start: 63
end: 1269
orientation: inline

BBa_K518002
type: ComponentDefinition
persistent identity: BBa K518002
display id: BBa_K518002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Firefly-renilla dual luciferase assay kit
creator: Masato Ohgishi
created: 2011-09-14T11:00:00Z
modified: 2015-05-08T01:12:33Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _683_
m user id: 0
owner id: 8305
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Luciferase assay is one of the most popular reporter assay system for quantitatively measuring the function of promoters and other cis-elements. The wide-ranging and quantitative detection is the prominent feature of this assay; light emmision from 10~(-18) ― 10~(-11) mol protein can be quantitatively measured using luminometer (see our results). A promoter or other cis-element to be analysed can be ligated above this part. Its effect will be measured as an altered firefly luciferase expression. Meanwhile, renilla luciferase expression can be used to as an internal control.
mutable notes: None
mutable provenance: From BioBrick parts.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Measurement
component: BBa_B0014
component: BBa_B0032
component: BBa_J52008
component: BBa_J23118
component: BBa_I712019
sequence annotation: BBa_B0014
sequence annotation: BBa_B0032
sequence annotation: BBa_I712019
sequence annotation: BBa_J23118
sequence annotation: BBa_J52008
sequence: https://synbiohub.org/public/igem/BBa_K518002_sequence/1

BBa_B0014
type: Component
persistent identity: component2128885
display id: component2128885
version: 1
top level: BBa_K518002
access: public
definition: https://synbiohub.org/public/igem/BBa_B0014/1

BBa_B0032
type: Component
persistent identity: component2128888
display id: component2128888
version: 1
top level: BBa_K518002
access: public
definition: https://synbiohub.org/public/igem/BBa_B0032/1

BBa_J52008
type: Component
persistent identity: component2128892
display id: component2128892
version: 1
top level: BBa_K518002
access: public
definition: https://synbiohub.org/public/igem/BBa_J52008/1

BBa_J23118
type: Component
persistent identity: component2128886
display id: component2128886
version: 1
top level: BBa_K518002
access: public
definition: https://synbiohub.org/public/igem/BBa_J23118/1

BBa_B0014
type: Component
persistent identity: component2128899
display id: component2128899
version: 1
top level: BBa_K518002
access: public
definition: https://synbiohub.org/public/igem/BBa_B0014/1

BBa_B0032
type: Component
persistent identity: component2128876
display id: component2128876
version: 1
top level: BBa_K518002
access: public
definition: https://synbiohub.org/public/igem/BBa_B0032/1

BBa_I712019
type: Component
persistent identity: component2128878
display id: component2128878
version: 1
top level: BBa_K518002
access: public
definition: https://synbiohub.org/public/igem/BBa_I712019/1

BBa_B0014
type: SequenceAnnotation
persistent identity: annotation2128885
display id: annotation2128885
version: 1
top level: BBa_K518002
location: https://synbiohub.org/public/igem/BBa_K518002/annotation2128885/range2128885/1
component: BBa_B0014

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type: Range
persistent identity: range2128885
display id: range2128885
version: 1
direction: direction/reverse
top level: BBa_K518002
start: 1681
end: 1775
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation2128876
display id: annotation2128876
version: 1
top level: BBa_K518002
location: https://synbiohub.org/public/igem/BBa_K518002/annotation2128876/range2128876/1
component: BBa_B0032

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type: Range
persistent identity: range2128876
display id: range2128876
version: 1
direction: direction/forward
top level: BBa_K518002
start: 1
end: 13
orientation: inline

BBa_B0014
type: SequenceAnnotation
persistent identity: annotation2128899
display id: annotation2128899
version: 1
top level: BBa_K518002
location: https://synbiohub.org/public/igem/BBa_K518002/annotation2128899/range2128899/1
component: BBa_B0014

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type: Range
persistent identity: range2128899
display id: range2128899
version: 1
direction: direction/reverse
top level: BBa_K518002
start: 2790
end: 2884
orientation: inline

BBa_I712019
type: SequenceAnnotation
persistent identity: annotation2128878
display id: annotation2128878
version: 1
top level: BBa_K518002
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BBa_K1379002
type: ComponentDefinition
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display id: BBa_K1379002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PcelA-E0240 (Measurement Kit for PcelA)
creator: Jordy Evan, Nadia Benedicta, Angeline Widjaja
created: 2014-09-21T11:00:00Z
modified: 2015-05-08T01:10:12Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1756_
m user id: 0
owner id: 21235
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: SigmaX-dependent promoter initiating the transcription of GFP
mutable notes: N/A
mutable provenance: The promoter is found genomic sequence of Streptococcus pneumoniae NCTC7465 strain, while the RBS+GFP+DTT is BBa_E0240
owned by: james
owned by: myers
star: false
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role: SO:0000804
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display id: BBa_J07027
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: ToxR'::PhoA (with promoter and RBS and terminator)
creator: jenmitch
created: 2005-07-13T11:00:00Z
modified: 2015-08-31T04:08:19Z
discontinued: false
dominant: false
experience: experience/None
group u list: _21_
m user id: 0
owner id: 164
owning group id: 21
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This part is a positive control for ToxR' dimerization.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Measurement
component: BBa_J07075
component: BBa_R0040
component: BBa_B0012
component: BBa_B0010
component: BBa_B0030
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sequence annotation: BBa_J07009
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description: R0010.E0021.B0011.E0031
creator: Caitlin Conboy
created: 2004-04-22T11:00:00Z
modified: 2015-08-31T04:07:44Z
discontinued: false
dominant: false
group u list: _1_
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sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Terminator Characterization Construct
owned by: james
owned by: myers
star: false
type: DnaRegion
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component: BBa_E0031
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type: ComponentDefinition
persistent identity: BBa I13529
display id: BBa_I13529
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Screening Plasmid 2.1
creator: Josh Michener
created: 2005-07-28T11:00:00Z
modified: 2015-08-31T04:07:35Z
discontinued: false
dominant: false
group u list: _11_
m user id: 0
owner id: 253
owning group id: 6
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Measurement
component: BBa_B0034
component: BBa_E1010
component: BBa_B0010
component: BBa_I0500
component: BBa_I13455
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sequence annotation: BBa_B0010
sequence annotation: BBa_B0034
sequence annotation: BBa_E0040
sequence annotation: BBa_I13455
sequence annotation: BBa_B0012
sequence annotation: BBa_E1010
sequence annotation: BBa_I0500
sequence: https://synbiohub.org/public/igem/BBa_I13529_sequence/1

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display id: BBa_I13114
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: High Output control (lac QPI)
creator: Barry Canton
created: 2004-07-20T11:00:00Z
modified: 2015-08-31T04:07:33Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_6_
m user id: 0
owner id: 135
owning group id: 7
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status: status/Unavailable
bookmark: false
mutable description: terminator upstream, no promoter
owned by: james
owned by: myers
star: false
type: DnaRegion
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role: partType/Measurement
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definition: https://synbiohub.org/public/igem/BBa_B0015/1

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access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

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type: SequenceAnnotation
persistent identity: annotation2231551
display id: annotation2231551
version: 1
top level: BBa_I13114
location: https://synbiohub.org/public/igem/BBa_I13114/annotation2231551/range2231551/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_I13114/annotation2231551/range2231551/1
type: Range
persistent identity: range2231551
display id: range2231551
version: 1
direction: direction/reverse
top level: BBa_I13114
start: 1
end: 129
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2231560
display id: annotation2231560
version: 1
top level: BBa_I13114
location: https://synbiohub.org/public/igem/BBa_I13114/annotation2231560/range2231560/1
component: BBa_B0015

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type: Range
persistent identity: range2231560
display id: range2231560
version: 1
direction: direction/reverse
top level: BBa_I13114
start: 1317
end: 1445
orientation: inline

BBa_I13021
type: SequenceAnnotation
persistent identity: annotation2231581
display id: annotation2231581
version: 1
top level: BBa_I13114
location: https://synbiohub.org/public/igem/BBa_I13114/annotation2231581/range2231581/1
component: BBa_I13021

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type: Range
persistent identity: range2231581
display id: range2231581
version: 1
direction: direction/reverse
top level: BBa_I13114
start: 138
end: 2394
orientation: inline

BBa_M30005
type: ComponentDefinition
persistent identity: BBa M30005
display id: BBa_M30005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: -- No description --
creator: natalie kuldell
created: 2005-12-04T12:00:00Z
modified: 2015-05-08T01:13:59Z
discontinued: false
dominant: true
group u list: _45_1_
m user id: 0
owner id: 314
owning group id: 1
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Positively regulated, OmpR-controlled promoter from OmpC gene (R0082) directing transcription of P22 c2 gene. The P22 c2 protein represses expression of mRFP (M30001). Constructed with standard assembly method except R0082 was isolated using VF and VR-directed PCR. Consequently, there may be silent mutations that were inadvertently introduced. A second isolate from this construction was saved as M30006.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Measurement
component: BBa_R0053
component: BBa_R0082
component: BBa_B0034
component: BBa_B0010
component: BBa_B0012
component: BBa_C0053
component: BBa_E1010
sequence annotation: BBa_B0034
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_R0053
sequence annotation: BBa_R0082
sequence annotation: BBa_E1010
sequence annotation: BBa_C0053
sequence: https://synbiohub.org/public/igem/BBa_M30005_sequence/1

BBa_R0053
type: Component
persistent identity: component1758199
display id: component1758199
version: 1
top level: BBa_M30005
access: public
definition: https://synbiohub.org/public/igem/BBa_R0053/1

BBa_R0082
type: Component
persistent identity: component1758145
display id: component1758145
version: 1
top level: BBa_M30005
access: public
definition: https://synbiohub.org/public/igem/BBa_R0082/1

BBa_B0034
type: Component
persistent identity: component1758150
display id: component1758150
version: 1
top level: BBa_M30005
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0010
type: Component
persistent identity: component1758170
display id: component1758170
version: 1
top level: BBa_M30005
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0012
type: Component
persistent identity: component1758180
display id: component1758180
version: 1
top level: BBa_M30005
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0034
type: Component
persistent identity: component1758209
display id: component1758209
version: 1
top level: BBa_M30005
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_C0053
type: Component
persistent identity: component1758162
display id: component1758162
version: 1
top level: BBa_M30005
access: public
definition: https://synbiohub.org/public/igem/BBa_C0053/1

BBa_E1010
type: Component
persistent identity: component1758216
display id: component1758216
version: 1
top level: BBa_M30005
access: public
definition: https://synbiohub.org/public/igem/BBa_E1010/1

BBa_B0010
type: Component
persistent identity: component1758222
display id: component1758222
version: 1
top level: BBa_M30005
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0012
type: Component
persistent identity: component1758232
display id: component1758232
version: 1
top level: BBa_M30005
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1758150
display id: annotation1758150
version: 1
top level: BBa_M30005
location: https://synbiohub.org/public/igem/BBa_M30005/annotation1758150/range1758150/1
component: BBa_B0034

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type: Range
persistent identity: range1758150
display id: range1758150
version: 1
direction: direction/forward
top level: BBa_M30005
start: 117
end: 128
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1758222
display id: annotation1758222
version: 1
top level: BBa_M30005
location: https://synbiohub.org/public/igem/BBa_M30005/annotation1758222/range1758222/1
component: BBa_B0010

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type: Range
persistent identity: range1758222
display id: range1758222
version: 1
direction: direction/forward
top level: BBa_M30005
start: 1786
end: 1865
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1758180
display id: annotation1758180
version: 1
top level: BBa_M30005
location: https://synbiohub.org/public/igem/BBa_M30005/annotation1758180/range1758180/1
component: BBa_B0012

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type: Range
persistent identity: range1758180
display id: range1758180
version: 1
direction: direction/forward
top level: BBa_M30005
start: 943
end: 983
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1758209
display id: annotation1758209
version: 1
top level: BBa_M30005
location: https://synbiohub.org/public/igem/BBa_M30005/annotation1758209/range1758209/1
component: BBa_B0034

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type: Range
persistent identity: range1758209
display id: range1758209
version: 1
direction: direction/forward
top level: BBa_M30005
start: 1054
end: 1065
orientation: inline

BBa_R0053
type: SequenceAnnotation
persistent identity: annotation1758199
display id: annotation1758199
version: 1
top level: BBa_M30005
location: https://synbiohub.org/public/igem/BBa_M30005/annotation1758199/range1758199/1
component: BBa_R0053

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type: Range
persistent identity: range1758199
display id: range1758199
version: 1
direction: direction/forward
top level: BBa_M30005
start: 992
end: 1045
orientation: inline

BBa_R0082
type: SequenceAnnotation
persistent identity: annotation1758145
display id: annotation1758145
version: 1
top level: BBa_M30005
location: https://synbiohub.org/public/igem/BBa_M30005/annotation1758145/range1758145/1
component: BBa_R0082

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type: Range
persistent identity: range1758145
display id: range1758145
version: 1
direction: direction/forward
top level: BBa_M30005
start: 1
end: 108
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1758232
display id: annotation1758232
version: 1
top level: BBa_M30005
location: https://synbiohub.org/public/igem/BBa_M30005/annotation1758232/range1758232/1
component: BBa_B0012

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type: Range
persistent identity: range1758232
display id: range1758232
version: 1
direction: direction/forward
top level: BBa_M30005
start: 1874
end: 1914
orientation: inline

BBa_E1010
type: SequenceAnnotation
persistent identity: annotation1758216
display id: annotation1758216
version: 1
top level: BBa_M30005
location: https://synbiohub.org/public/igem/BBa_M30005/annotation1758216/range1758216/1
component: BBa_E1010

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type: Range
persistent identity: range1758216
display id: range1758216
version: 1
direction: direction/forward
top level: BBa_M30005
start: 1072
end: 1752
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1758170
display id: annotation1758170
version: 1
top level: BBa_M30005
location: https://synbiohub.org/public/igem/BBa_M30005/annotation1758170/range1758170/1
component: BBa_B0010

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type: Range
persistent identity: range1758170
display id: range1758170
version: 1
direction: direction/forward
top level: BBa_M30005
start: 855
end: 934
orientation: inline

BBa_C0053
type: SequenceAnnotation
persistent identity: annotation1758162
display id: annotation1758162
version: 1
top level: BBa_M30005
location: https://synbiohub.org/public/igem/BBa_M30005/annotation1758162/range1758162/1
component: BBa_C0053

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type: Range
persistent identity: range1758162
display id: range1758162
version: 1
direction: direction/forward
top level: BBa_M30005
start: 135
end: 821
orientation: inline

BBa_K1379003
type: ComponentDefinition
persistent identity: BBa K1379003
display id: BBa_K1379003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PcomFA-E0240 (Measurement Kit for PcomFA)
creator: Ng Siu Wang Edward, Jason Jang, Joo Min Jung
created: 2014-09-21T11:00:00Z
modified: 2015-05-08T01:10:12Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1756_
m user id: 0
owner id: 21235
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: SigmaX-dependent GFP generator
mutable notes: N/A
mutable provenance: The promoter is found genomic sequence of Streptococcus pneumoniae NCTC7465 strain, while the RBS+GFP+DTT is BBa_E0240
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Measurement
component: BBa_E0240
component: BBa_K1379001
sequence annotation: BBa_E0240
sequence annotation: BBa_K1379001
sequence: https://synbiohub.org/public/igem/BBa_K1379003_sequence/1

BBa_E0240
type: Component
persistent identity: component2421671
display id: component2421671
version: 1
top level: BBa_K1379003
access: public
definition: https://synbiohub.org/public/igem/BBa_E0240/1

BBa_K1379001
type: Component
persistent identity: component2421660
display id: component2421660
version: 1
top level: BBa_K1379003
access: public
definition: https://synbiohub.org/public/igem/BBa_K1379001/1

BBa_E0240
type: SequenceAnnotation
persistent identity: annotation2421671
display id: annotation2421671
version: 1
top level: BBa_K1379003
location: https://synbiohub.org/public/igem/BBa_K1379003/annotation2421671/range2421671/1
component: BBa_E0240

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type: Range
persistent identity: range2421671
display id: range2421671
version: 1
direction: direction/reverse
top level: BBa_K1379003
start: 169
end: 1044
orientation: inline

BBa_K1379001
type: SequenceAnnotation
persistent identity: annotation2421660
display id: annotation2421660
version: 1
top level: BBa_K1379003
location: https://synbiohub.org/public/igem/BBa_K1379003/annotation2421660/range2421660/1
component: BBa_K1379001

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type: Range
persistent identity: range2421660
display id: range2421660
version: 1
direction: direction/reverse
top level: BBa_K1379003
start: 1
end: 160
orientation: inline

BBa_I13644
type: ComponentDefinition
persistent identity: BBa I13644
display id: BBa_I13644
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Cascaded Lac (medium) and Tet (medium) QPIs with double terminator as input
creator: mit
created: 2004-07-19T11:00:00Z
modified: 2015-08-31T04:07:36Z
discontinued: false
dominant: false
experience: experience/None
group u list: _6_
m user id: 0
owner id: 101
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Measurement
component: BBa_Q01400
component: BBa_B0015
component: BBa_Q01121
sequence annotation: BBa_Q01121
sequence annotation: BBa_B0015
sequence annotation: BBa_Q01400
sequence: https://synbiohub.org/public/igem/BBa_I13644_sequence/1

BBa_Q01400
type: Component
persistent identity: component2232512
display id: component2232512
version: 1
top level: BBa_I13644
access: public
definition: https://synbiohub.org/public/igem/BBa_Q01400/1

BBa_B0015
type: Component
persistent identity: component2232475
display id: component2232475
version: 1
top level: BBa_I13644
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_Q01121
type: Component
persistent identity: component2232494
display id: component2232494
version: 1
top level: BBa_I13644
access: public
definition: https://synbiohub.org/public/igem/BBa_Q01121/1

BBa_Q01121
type: SequenceAnnotation
persistent identity: annotation2232494
display id: annotation2232494
version: 1
top level: BBa_I13644
location: https://synbiohub.org/public/igem/BBa_I13644/annotation2232494/range2232494/1
component: BBa_Q01121

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type: Range
persistent identity: range2232494
display id: range2232494
version: 1
direction: direction/reverse
top level: BBa_I13644
start: 138
end: 1510
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2232475
display id: annotation2232475
version: 1
top level: BBa_I13644
location: https://synbiohub.org/public/igem/BBa_I13644/annotation2232475/range2232475/1
component: BBa_B0015

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type: Range
persistent identity: range2232475
display id: range2232475
version: 1
direction: direction/reverse
top level: BBa_I13644
start: 1
end: 129
orientation: inline

BBa_Q01400
type: SequenceAnnotation
persistent identity: annotation2232512
display id: annotation2232512
version: 1
top level: BBa_I13644
location: https://synbiohub.org/public/igem/BBa_I13644/annotation2232512/range2232512/1
component: BBa_Q01400

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type: Range
persistent identity: range2232512
display id: range2232512
version: 1
direction: direction/reverse
top level: BBa_I13644
start: 1519
end: 2422
orientation: inline

BBa_I13009
type: ComponentDefinition
persistent identity: BBa I13009
display id: BBa_I13009
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS Test R0011.B0032.E0130
creator: jasonk
created: 2004-06-30T11:00:00Z
modified: 2015-08-31T04:07:32Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_6_
m user id: 0
owner id: 135
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Parts I13007-I130014 are RBS test constructs for RBS characterization. Each consists of Lac or Tet promoter, one of the four RBS's B0030-B0033, a YFP coding protein, and B0015 terminator.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Measurement
component: BBa_E0130
component: BBa_R0011
component: BBa_B0032
sequence annotation: BBa_R0011
sequence annotation: BBa_B0032
sequence annotation: BBa_E0130
sequence: https://synbiohub.org/public/igem/BBa_I13009_sequence/1

BBa_E0130
type: Component
persistent identity: component2221446
display id: component2221446
version: 1
top level: BBa_I13009
access: public
definition: https://synbiohub.org/public/igem/BBa_E0130/1

BBa_R0011
type: Component
persistent identity: component2221431
display id: component2221431
version: 1
top level: BBa_I13009
access: public
definition: https://synbiohub.org/public/igem/BBa_R0011/1

BBa_B0032
type: Component
persistent identity: component2221437
display id: component2221437
version: 1
top level: BBa_I13009
access: public
definition: https://synbiohub.org/public/igem/BBa_B0032/1

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation2221431
display id: annotation2221431
version: 1
top level: BBa_I13009
location: https://synbiohub.org/public/igem/BBa_I13009/annotation2221431/range2221431/1
component: BBa_R0011

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type: Range
persistent identity: range2221431
display id: range2221431
version: 1
direction: direction/forward
top level: BBa_I13009
start: 1
end: 54
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation2221437
display id: annotation2221437
version: 1
top level: BBa_I13009
location: https://synbiohub.org/public/igem/BBa_I13009/annotation2221437/range2221437/1
component: BBa_B0032

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type: Range
persistent identity: range2221437
display id: range2221437
version: 1
direction: direction/forward
top level: BBa_I13009
start: 64
end: 76
orientation: inline

BBa_E0130
type: SequenceAnnotation
persistent identity: annotation2221446
display id: annotation2221446
version: 1
top level: BBa_I13009
location: https://synbiohub.org/public/igem/BBa_I13009/annotation2221446/range2221446/1
component: BBa_E0130

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type: Range
persistent identity: range2221446
display id: range2221446
version: 1
direction: direction/reverse
top level: BBa_I13009
start: 83
end: 942
orientation: inline

BBa_I13651
type: ComponentDefinition
persistent identity: BBa I13651
display id: BBa_I13651
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Cascaded Tet (weak) and Lac (medium) QPIs with constitutive promoter as input
creator: mit
created: 2004-07-19T11:00:00Z
modified: 2015-08-31T04:07:36Z
discontinued: false
dominant: false
experience: experience/None
group u list: _6_
m user id: 0
owner id: 101
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Measurement
component: BBa_R0051
component: BBa_Q01121
component: BBa_Q03400
sequence annotation: BBa_Q01121
sequence annotation: BBa_R0051
sequence annotation: BBa_Q03400
sequence: https://synbiohub.org/public/igem/BBa_I13651_sequence/1

BBa_R0051
type: Component
persistent identity: component2231250
display id: component2231250
version: 1
top level: BBa_I13651
access: public
definition: https://synbiohub.org/public/igem/BBa_R0051/1

BBa_Q01121
type: Component
persistent identity: component2231291
display id: component2231291
version: 1
top level: BBa_I13651
access: public
definition: https://synbiohub.org/public/igem/BBa_Q01121/1

BBa_Q03400
type: Component
persistent identity: component2231272
display id: component2231272
version: 1
top level: BBa_I13651
access: public
definition: https://synbiohub.org/public/igem/BBa_Q03400/1

BBa_Q01121
type: SequenceAnnotation
persistent identity: annotation2231291
display id: annotation2231291
version: 1
top level: BBa_I13651
location: https://synbiohub.org/public/igem/BBa_I13651/annotation2231291/range2231291/1
component: BBa_Q01121

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type: Range
persistent identity: range2231291
display id: range2231291
version: 1
direction: direction/reverse
top level: BBa_I13651
start: 967
end: 2339
orientation: inline

BBa_R0051
type: SequenceAnnotation
persistent identity: annotation2231250
display id: annotation2231250
version: 1
top level: BBa_I13651
location: https://synbiohub.org/public/igem/BBa_I13651/annotation2231250/range2231250/1
component: BBa_R0051

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type: Range
persistent identity: range2231250
display id: range2231250
version: 1
direction: direction/forward
top level: BBa_I13651
start: 1
end: 49
orientation: inline

BBa_Q03400
type: SequenceAnnotation
persistent identity: annotation2231272
display id: annotation2231272
version: 1
top level: BBa_I13651
location: https://synbiohub.org/public/igem/BBa_I13651/annotation2231272/range2231272/1
component: BBa_Q03400

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type: Range
persistent identity: range2231272
display id: range2231272
version: 1
direction: direction/reverse
top level: BBa_I13651
start: 58
end: 958
orientation: inline

BBa_I13625
type: ComponentDefinition
persistent identity: BBa I13625
display id: BBa_I13625
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Cascaded Lac (medium) and Tet (elowitz) QPIs
creator: mit
created: 2004-07-19T11:00:00Z
modified: 2015-08-31T04:07:36Z
discontinued: false
dominant: false
experience: experience/None
group u list: _6_
m user id: 0
owner id: 101
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Measurement
component: BBa_Q01121
component: BBa_Q04400
sequence annotation: BBa_Q01121
sequence annotation: BBa_Q04400
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display id: component2229089
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access: public
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BBa_I13007
type: ComponentDefinition
persistent identity: BBa I13007
display id: BBa_I13007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS Test R0011.B0030.E0130
creator: jasonk
created: 2004-06-30T11:00:00Z
modified: 2015-08-31T04:07:32Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_6_
m user id: 0
owner id: 135
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Parts I13007-I130014 are RBS test constructs for RBS characterization. Each consists of Lac or Tet promoter, one of the four RBS's B0030-B0033, a YFP coding protein, and B0015 terminator.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
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component: BBa_R0011
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component: BBa_B0030
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sequence annotation: BBa_E0130
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sequence: https://synbiohub.org/public/igem/BBa_I13007_sequence/1

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BBa_B0030
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BBa_I6007
type: ComponentDefinition
persistent identity: BBa I6007
display id: BBa_I6007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: QPI Curve Test I0500.E0422.I0500.Q04400.E0432
creator: Caitlin Conboy, Jennifer Braff
created: 2003-12-05T12:00:00Z
modified: 2015-08-31T04:07:42Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: QPI characterization assembly
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000804
role: partType/Measurement
component: BBa_B0012
component: BBa_B0034
component: BBa_B0010
component: BBa_E0032
component: BBa_I0500
component: BBa_R0040
component: BBa_E0022
component: BBa_C0040
sequence annotation: BBa_I0500
sequence annotation: BBa_B0010
sequence annotation: BBa_C0040
sequence annotation: BBa_B0034
sequence annotation: BBa_R0040
sequence annotation: BBa_B0012
sequence annotation: BBa_E0022
sequence annotation: BBa_E0032
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BBa_B0012
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BBa_B0034
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BBa_B0010
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BBa_B0010
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BBa_B0034
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BBa_B0010
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BBa_E0032
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BBa_B0012
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BBa_B0010
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BBa_B0012
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BBa_I0412
type: ComponentDefinition
persistent identity: BBa I0412
display id: BBa_I0412
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS Test R0040.B0034.E0030.B0015
creator: jcbraff
created: 2004-06-13T11:00:00Z
modified: 2015-08-31T04:07:28Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_1_
m user id: 0
owner id: 61
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: R0040.B0034.E0030.B0015 RBS Test
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Measurement
component: BBa_B0010
component: BBa_B0012
component: BBa_B0034
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component: BBa_E0030
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BBa_B0010
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BBa_B0012
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version: 1
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BBa_B0034
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BBa_R0040
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BBa_E0030
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ZF test
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version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Zinc Finger Protein Tester
creator: NOR-Group
created: 2005-08-28T11:00:00Z
modified: 2015-08-31T04:08:15Z
discontinued: false
dominant: false
group u list: _34_
m user id: 0
owner id: 400
owning group id: 34
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Tester to investigate zinc finger proteins (ZFP) as a negative regulator of expression. Lac promoter controls expression of a ZFP and a reporter (i.e. RFP) to confirm expression. Lambda promoter constitutively expresses GFP unless ZFP binds to the binding site after the lambda promoter.
mutable notes: Promotor RBS Zinc_Finger RBS RFP Terminator Promotor ZF-Binding_site1 ZF-Binding_site2 RBS GFP Terminator
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Measurement
component: BBa_J05100
component: BBa_R0010
component: BBa_I13507
component: BBa_B0034
component: BBa_J05211
component: BBa_I13504
component: BBa_R0051
sequence annotation: BBa_J05211
sequence annotation: BBa_R0010
sequence annotation: BBa_I13507
sequence annotation: BBa_R0051
sequence annotation: BBa_J05100
sequence annotation: BBa_B0034
sequence annotation: BBa_I13504
sequence: https://synbiohub.org/public/igem/BBa_J05000_sequence/1

BBa_J05100
type: Component
persistent identity: component2233363
display id: component2233363
version: 1
top level: ZF test
access: public
definition: https://synbiohub.org/public/igem/BBa_J05100/1

BBa_R0010
type: Component
persistent identity: component2233354
display id: component2233354
version: 1
top level: ZF test
access: public
definition: https://synbiohub.org/public/igem/BBa_R0010/1

BBa_I13507
type: Component
persistent identity: component2233375
display id: component2233375
version: 1
top level: ZF test
access: public
definition: https://synbiohub.org/public/igem/BBa_I13507/1

BBa_B0034
type: Component
persistent identity: component2233362
display id: component2233362
version: 1
top level: ZF test
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_J05211
type: Component
persistent identity: component2233381
display id: component2233381
version: 1
top level: ZF test
access: public
definition: https://synbiohub.org/public/igem/BBa_J05211/1

BBa_I13504
type: Component
persistent identity: component2233391
display id: component2233391
version: 1
top level: ZF test
access: public
definition: https://synbiohub.org/public/igem/BBa_I13504/1

BBa_R0051
type: Component
persistent identity: component2233377
display id: component2233377
version: 1
top level: ZF test
access: public
definition: https://synbiohub.org/public/igem/BBa_R0051/1

BBa_J05211
type: SequenceAnnotation
persistent identity: annotation2233381
display id: annotation2233381
version: 1
top level: ZF test
location: https://synbiohub.org/public/igem/BBa_J05000/annotation2233381/range2233381/1
component: BBa_J05211

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type: Range
persistent identity: range2233381
display id: range2233381
version: 1
direction: direction/reverse
top level: ZF test
start: 1470
end: 1493
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation2233354
display id: annotation2233354
version: 1
top level: ZF test
location: https://synbiohub.org/public/igem/BBa_J05000/annotation2233354/range2233354/1
component: BBa_R0010

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type: Range
persistent identity: range2233354
display id: range2233354
version: 1
direction: direction/forward
top level: ZF test
start: 1
end: 200
orientation: inline

BBa_I13507
type: SequenceAnnotation
persistent identity: annotation2233375
display id: annotation2233375
version: 1
top level: ZF test
location: https://synbiohub.org/public/igem/BBa_J05000/annotation2233375/range2233375/1
component: BBa_I13507

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type: Range
persistent identity: range2233375
display id: range2233375
version: 1
direction: direction/reverse
top level: ZF test
start: 544
end: 1404
orientation: inline

BBa_R0051
type: SequenceAnnotation
persistent identity: annotation2233377
display id: annotation2233377
version: 1
top level: ZF test
location: https://synbiohub.org/public/igem/BBa_J05000/annotation2233377/range2233377/1
component: BBa_R0051

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type: Range
persistent identity: range2233377
display id: range2233377
version: 1
direction: direction/forward
top level: ZF test
start: 1413
end: 1461
orientation: inline

BBa_J05100
type: SequenceAnnotation
persistent identity: annotation2233363
display id: annotation2233363
version: 1
top level: ZF test
location: https://synbiohub.org/public/igem/BBa_J05000/annotation2233363/range2233363/1
component: BBa_J05100

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type: Range
persistent identity: range2233363
display id: range2233363
version: 1
direction: direction/reverse
top level: ZF test
start: 227
end: 535
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2233362
display id: annotation2233362
version: 1
top level: ZF test
location: https://synbiohub.org/public/igem/BBa_J05000/annotation2233362/range2233362/1
component: BBa_B0034

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type: Range
persistent identity: range2233362
display id: range2233362
version: 1
direction: direction/reverse
top level: ZF test
start: 209
end: 220
orientation: inline

BBa_I13504
type: SequenceAnnotation
persistent identity: annotation2233391
display id: annotation2233391
version: 1
top level: ZF test
location: https://synbiohub.org/public/igem/BBa_J05000/annotation2233391/range2233391/1
component: BBa_I13504

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type: Range
persistent identity: range2233391
display id: range2233391
version: 1
direction: direction/reverse
top level: ZF test
start: 1502
end: 2376
orientation: inline

BBa_I12244
type: ComponentDefinition
persistent identity: BBa I12244
display id: BBa_I12244
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Test of AraC protein generator 2
creator: rwald
created: 2004-08-31T11:00:00Z
modified: 2015-08-31T04:07:32Z
discontinued: false
dominant: false
experience: experience/None
group u list: _3_
m user id: 0
owner id: 120
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This component tests the I12222 TetR protein generator. It uses the I0500 promoter region for the test.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Measurement
component: BBa_B0010
component: BBa_I12205
component: BBa_B0032
component: BBa_I0500
component: BBa_B0012
component: BBa_B0034
component: BBa_E0022
component: BBa_R0053
sequence annotation: BBa_B0032
sequence annotation: BBa_B0010
sequence annotation: BBa_E0022
sequence annotation: BBa_I12205
sequence annotation: BBa_B0012
sequence annotation: BBa_B0034
sequence annotation: BBa_I0500
sequence annotation: BBa_R0053
sequence: https://synbiohub.org/public/igem/BBa_I12244_sequence/1

BBa_B0010
type: Component
persistent identity: component1063358
display id: component1063358
version: 1
top level: BBa_I12244
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_I12205
type: Component
persistent identity: component1063352
display id: component1063352
version: 1
top level: BBa_I12244
access: public
definition: https://synbiohub.org/public/igem/BBa_I12205/1

BBa_B0032
type: Component
persistent identity: component1063344
display id: component1063344
version: 1
top level: BBa_I12244
access: public
definition: https://synbiohub.org/public/igem/BBa_B0032/1

BBa_B0010
type: Component
persistent identity: component1063398
display id: component1063398
version: 1
top level: BBa_I12244
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_I0500
type: Component
persistent identity: component1063373
display id: component1063373
version: 1
top level: BBa_I12244
access: public
definition: https://synbiohub.org/public/igem/BBa_I0500/1

BBa_B0012
type: Component
persistent identity: component1063408
display id: component1063408
version: 1
top level: BBa_I12244
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0034
type: Component
persistent identity: component1063378
display id: component1063378
version: 1
top level: BBa_I12244
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_E0022
type: Component
persistent identity: component1063390
display id: component1063390
version: 1
top level: BBa_I12244
access: public
definition: https://synbiohub.org/public/igem/BBa_E0022/1

BBa_B0012
type: Component
persistent identity: component1063368
display id: component1063368
version: 1
top level: BBa_I12244
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_R0053
type: Component
persistent identity: component1063331
display id: component1063331
version: 1
top level: BBa_I12244
access: public
definition: https://synbiohub.org/public/igem/BBa_R0053/1

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation1063344
display id: annotation1063344
version: 1
top level: BBa_I12244
location: https://synbiohub.org/public/igem/BBa_I12244/annotation1063344/range1063344/1
component: BBa_B0032

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type: Range
persistent identity: range1063344
display id: range1063344
version: 1
direction: direction/forward
top level: BBa_I12244
start: 63
end: 75
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1063358
display id: annotation1063358
version: 1
top level: BBa_I12244
location: https://synbiohub.org/public/igem/BBa_I12244/annotation1063358/range1063358/1
component: BBa_B0010

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type: Range
persistent identity: range1063358
display id: range1063358
version: 1
direction: direction/forward
top level: BBa_I12244
start: 997
end: 1076
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1063398
display id: annotation1063398
version: 1
top level: BBa_I12244
location: https://synbiohub.org/public/igem/BBa_I12244/annotation1063398/range1063398/1
component: BBa_B0010

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type: Range
persistent identity: range1063398
display id: range1063398
version: 1
direction: direction/forward
top level: BBa_I12244
start: 3140
end: 3219
orientation: inline

BBa_E0022
type: SequenceAnnotation
persistent identity: annotation1063390
display id: annotation1063390
version: 1
top level: BBa_I12244
location: https://synbiohub.org/public/igem/BBa_I12244/annotation1063390/range1063390/1
component: BBa_E0022

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type: Range
persistent identity: range1063390
display id: range1063390
version: 1
direction: direction/forward
top level: BBa_I12244
start: 2370
end: 3131
orientation: inline

BBa_I12205
type: SequenceAnnotation
persistent identity: annotation1063352
display id: annotation1063352
version: 1
top level: BBa_I12244
location: https://synbiohub.org/public/igem/BBa_I12244/annotation1063352/range1063352/1
component: BBa_I12205

https://synbiohub.org/public/igem/BBa_I12244/annotation1063352/range1063352/1
type: Range
persistent identity: range1063352
display id: range1063352
version: 1
direction: direction/forward
top level: BBa_I12244
start: 82
end: 963
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1063408
display id: annotation1063408
version: 1
top level: BBa_I12244
location: https://synbiohub.org/public/igem/BBa_I12244/annotation1063408/range1063408/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I12244/annotation1063408/range1063408/1
type: Range
persistent identity: range1063408
display id: range1063408
version: 1
direction: direction/forward
top level: BBa_I12244
start: 3228
end: 3268
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1063378
display id: annotation1063378
version: 1
top level: BBa_I12244
location: https://synbiohub.org/public/igem/BBa_I12244/annotation1063378/range1063378/1
component: BBa_B0034

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type: Range
persistent identity: range1063378
display id: range1063378
version: 1
direction: direction/forward
top level: BBa_I12244
start: 2352
end: 2363
orientation: inline

BBa_I0500
type: SequenceAnnotation
persistent identity: annotation1063373
display id: annotation1063373
version: 1
top level: BBa_I12244
location: https://synbiohub.org/public/igem/BBa_I12244/annotation1063373/range1063373/1
component: BBa_I0500

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type: Range
persistent identity: range1063373
display id: range1063373
version: 1
direction: direction/forward
top level: BBa_I12244
start: 1134
end: 2343
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1063368
display id: annotation1063368
version: 1
top level: BBa_I12244
location: https://synbiohub.org/public/igem/BBa_I12244/annotation1063368/range1063368/1
component: BBa_B0012

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type: Range
persistent identity: range1063368
display id: range1063368
version: 1
direction: direction/forward
top level: BBa_I12244
start: 1085
end: 1125
orientation: inline

BBa_R0053
type: SequenceAnnotation
persistent identity: annotation1063331
display id: annotation1063331
version: 1
top level: BBa_I12244
location: https://synbiohub.org/public/igem/BBa_I12244/annotation1063331/range1063331/1
component: BBa_R0053

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type: Range
persistent identity: range1063331
display id: range1063331
version: 1
direction: direction/forward
top level: BBa_I12244
start: 1
end: 54
orientation: inline

BBa_I13013
type: ComponentDefinition
persistent identity: BBa I13013
display id: BBa_I13013
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS Test R0040.B0032.E0130
creator: jasonk
created: 2004-06-30T11:00:00Z
modified: 2015-08-31T04:07:32Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_6_
m user id: 0
owner id: 135
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Parts I13007-I130014 are RBS test constructs for RBS characterization. Each consists of Lac or Tet promoter, one of the four RBS's B0030-B0033, a YFP coding protein, and B0015 terminator.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Measurement
component: BBa_R0040
component: BBa_B0032
component: BBa_B0010
component: BBa_B0012
component: BBa_E0030
sequence annotation: BBa_B0012
sequence annotation: BBa_R0040
sequence annotation: BBa_B0032
sequence annotation: BBa_B0010
sequence annotation: BBa_E0030
sequence: https://synbiohub.org/public/igem/BBa_I13013_sequence/1

BBa_R0040
type: Component
persistent identity: component946842
display id: component946842
version: 1
top level: BBa_I13013
access: public
definition: https://synbiohub.org/public/igem/BBa_R0040/1

BBa_B0032
type: Component
persistent identity: component946853
display id: component946853
version: 1
top level: BBa_I13013
access: public
definition: https://synbiohub.org/public/igem/BBa_B0032/1

BBa_B0010
type: Component
persistent identity: component946861
display id: component946861
version: 1
top level: BBa_I13013
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0012
type: Component
persistent identity: component946871
display id: component946871
version: 1
top level: BBa_I13013
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_E0030
type: Component
persistent identity: component946856
display id: component946856
version: 1
top level: BBa_I13013
access: public
definition: https://synbiohub.org/public/igem/BBa_E0030/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation946871
display id: annotation946871
version: 1
top level: BBa_I13013
location: https://synbiohub.org/public/igem/BBa_I13013/annotation946871/range946871/1
component: BBa_B0012

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type: Range
persistent identity: range946871
display id: range946871
version: 1
direction: direction/forward
top level: BBa_I13013
start: 901
end: 941
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation946842
display id: annotation946842
version: 1
top level: BBa_I13013
location: https://synbiohub.org/public/igem/BBa_I13013/annotation946842/range946842/1
component: BBa_R0040

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type: Range
persistent identity: range946842
display id: range946842
version: 1
direction: direction/forward
top level: BBa_I13013
start: 1
end: 54
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation946853
display id: annotation946853
version: 1
top level: BBa_I13013
location: https://synbiohub.org/public/igem/BBa_I13013/annotation946853/range946853/1
component: BBa_B0032

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type: Range
persistent identity: range946853
display id: range946853
version: 1
direction: direction/forward
top level: BBa_I13013
start: 63
end: 75
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation946861
display id: annotation946861
version: 1
top level: BBa_I13013
location: https://synbiohub.org/public/igem/BBa_I13013/annotation946861/range946861/1
component: BBa_B0010

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type: Range
persistent identity: range946861
display id: range946861
version: 1
direction: direction/forward
top level: BBa_I13013
start: 813
end: 892
orientation: inline

BBa_E0030
type: SequenceAnnotation
persistent identity: annotation946856
display id: annotation946856
version: 1
top level: BBa_I13013
location: https://synbiohub.org/public/igem/BBa_I13013/annotation946856/range946856/1
component: BBa_E0030

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type: Range
persistent identity: range946856
display id: range946856
version: 1
direction: direction/forward
top level: BBa_I13013
start: 82
end: 804
orientation: inline

BBa_I7121
type: ComponentDefinition
persistent identity: BBa I7121
display id: BBa_I7121
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: R0040.B0032.E0040.M0100.B0015
creator: jcbraff
created: 2005-05-16T11:00:00Z
modified: 2015-08-31T04:07:46Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 61
owning group id: 1
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: R0040.B0032.E0040.M0100.B0015
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Measurement
component: BBa_E0040
component: BBa_B0010
component: BBa_R0040
component: BBa_B0012
component: BBa_M0100
component: BBa_B0032
sequence annotation: BBa_B0010
sequence annotation: BBa_E0040
sequence annotation: BBa_B0032
sequence annotation: BBa_R0040
sequence annotation: BBa_B0012
sequence annotation: BBa_M0100
sequence: https://synbiohub.org/public/igem/BBa_I7121_sequence/1

BBa_E0040
type: Component
persistent identity: component1489242
display id: component1489242
version: 1
top level: BBa_I7121
access: public
definition: https://synbiohub.org/public/igem/BBa_E0040/1

BBa_B0010
type: Component
persistent identity: component1489249
display id: component1489249
version: 1
top level: BBa_I7121
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_R0040
type: Component
persistent identity: component1489228
display id: component1489228
version: 1
top level: BBa_I7121
access: public
definition: https://synbiohub.org/public/igem/BBa_R0040/1

BBa_B0012
type: Component
persistent identity: component1489259
display id: component1489259
version: 1
top level: BBa_I7121
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_M0100
type: Component
persistent identity: component1489244
display id: component1489244
version: 1
top level: BBa_I7121
access: public
definition: https://synbiohub.org/public/igem/BBa_M0100/1

BBa_B0032
type: Component
persistent identity: component1489239
display id: component1489239
version: 1
top level: BBa_I7121
access: public
definition: https://synbiohub.org/public/igem/BBa_B0032/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1489249
display id: annotation1489249
version: 1
top level: BBa_I7121
location: https://synbiohub.org/public/igem/BBa_I7121/annotation1489249/range1489249/1
component: BBa_B0010

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type: Range
persistent identity: range1489249
display id: range1489249
version: 1
direction: direction/forward
top level: BBa_I7121
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orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation1489242
display id: annotation1489242
version: 1
top level: BBa_I7121
location: https://synbiohub.org/public/igem/BBa_I7121/annotation1489242/range1489242/1
component: BBa_E0040

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persistent identity: range1489242
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BBa_B0032
type: SequenceAnnotation
persistent identity: annotation1489239
display id: annotation1489239
version: 1
top level: BBa_I7121
location: https://synbiohub.org/public/igem/BBa_I7121/annotation1489239/range1489239/1
component: BBa_B0032

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display id: range1489239
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BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1489228
display id: annotation1489228
version: 1
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component: BBa_R0040

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BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1489259
display id: annotation1489259
version: 1
top level: BBa_I7121
location: https://synbiohub.org/public/igem/BBa_I7121/annotation1489259/range1489259/1
component: BBa_B0012

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display id: range1489259
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end: 1031
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BBa_M0100
type: SequenceAnnotation
persistent identity: annotation1489244
display id: annotation1489244
version: 1
top level: BBa_I7121
location: https://synbiohub.org/public/igem/BBa_I7121/annotation1489244/range1489244/1
component: BBa_M0100

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persistent identity: range1489244
display id: range1489244
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BBa_I0410
type: ComponentDefinition
persistent identity: BBa I0410
display id: BBa_I0410
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS Test R0040.B0031.E0030.B0015
creator: jcbraff
created: 2004-06-13T11:00:00Z
modified: 2015-08-31T04:07:28Z
discontinued: false
dominant: true
experience: experience/None
group u list: _11_1_
m user id: 0
owner id: 61
owning group id: 7
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: R0040.B0031.E0030.B0015 RBS Test
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Measurement
component: BBa_B0010
component: BBa_B0012
component: BBa_E0030
component: BBa_R0040
component: BBa_B0031
sequence annotation: BBa_R0040
sequence annotation: BBa_B0012
sequence annotation: BBa_B0031
sequence annotation: BBa_B0010
sequence annotation: BBa_E0030
sequence: https://synbiohub.org/public/igem/BBa_I0410_sequence/1

BBa_B0010
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display id: component946208
version: 1
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access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0012
type: Component
persistent identity: component946218
display id: component946218
version: 1
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access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_E0030
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persistent identity: component946203
display id: component946203
version: 1
top level: BBa_I0410
access: public
definition: https://synbiohub.org/public/igem/BBa_E0030/1

BBa_R0040
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display id: component946193
version: 1
top level: BBa_I0410
access: public
definition: https://synbiohub.org/public/igem/BBa_R0040/1

BBa_B0031
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persistent identity: component946201
display id: component946201
version: 1
top level: BBa_I0410
access: public
definition: https://synbiohub.org/public/igem/BBa_B0031/1

BBa_R0040
type: SequenceAnnotation
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display id: annotation946193
version: 1
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component: BBa_R0040

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BBa_B0012
type: SequenceAnnotation
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version: 1
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location: https://synbiohub.org/public/igem/BBa_I0410/annotation946218/range946218/1
component: BBa_B0012

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BBa_B0010
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component: BBa_B0010

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display id: range946208
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start: 814
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BBa_E0030
type: SequenceAnnotation
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component: BBa_E0030

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end: 805
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BBa_M30006
type: ComponentDefinition
persistent identity: BBa M30006
display id: BBa_M30006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: -- No description --
creator: natalie kuldell
created: 2005-12-04T12:00:00Z
modified: 2015-05-08T01:13:59Z
discontinued: false
dominant: true
group u list: _45_1_
m user id: 0
owner id: 314
owning group id: 1
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Positively regulated, OmpR-controlled promoter from OmpC gene (R0082) directing transcription of P22 c2 gene. The P22 c2 protein represses expression of mRFP (M30001). Constructed with standard assembly method except R0082 was isolated using VF and VR-directed PCR. Consequently, there may be silent mutations that were inadvertently introduced. A second isolate from this construction was saved as M30005.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Measurement
component: BBa_B0010
component: BBa_R0053
component: BBa_B0034
component: BBa_C0053
component: BBa_B0012
component: BBa_E1010
component: BBa_R0082
sequence annotation: BBa_R0082
sequence annotation: BBa_B0034
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_E1010
sequence annotation: BBa_C0053
sequence annotation: BBa_R0053
sequence: https://synbiohub.org/public/igem/BBa_M30006_sequence/1

BBa_B0010
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display id: component1758409
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access: public
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BBa_R0053
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definition: https://synbiohub.org/public/igem/BBa_R0053/1

BBa_B0034
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access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_C0053
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access: public
definition: https://synbiohub.org/public/igem/BBa_C0053/1

BBa_B0012
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display id: component1758471
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top level: BBa_M30006
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0012
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display id: component1758419
version: 1
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access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_E1010
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display id: component1758455
version: 1
top level: BBa_M30006
access: public
definition: https://synbiohub.org/public/igem/BBa_E1010/1

BBa_B0034
type: Component
persistent identity: component1758389
display id: component1758389
version: 1
top level: BBa_M30006
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_R0082
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display id: component1758384
version: 1
top level: BBa_M30006
access: public
definition: https://synbiohub.org/public/igem/BBa_R0082/1

BBa_B0010
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persistent identity: component1758461
display id: component1758461
version: 1
top level: BBa_M30006
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_R0082
type: SequenceAnnotation
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display id: annotation1758384
version: 1
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component: BBa_R0082

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type: Range
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display id: range1758384
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direction: direction/forward
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start: 1
end: 108
orientation: inline

BBa_B0034
type: SequenceAnnotation
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version: 1
top level: BBa_M30006
location: https://synbiohub.org/public/igem/BBa_M30006/annotation1758448/range1758448/1
component: BBa_B0034

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persistent identity: range1758448
display id: range1758448
version: 1
direction: direction/forward
top level: BBa_M30006
start: 1054
end: 1065
orientation: inline

BBa_B0010
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version: 1
top level: BBa_M30006
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display id: range1758409
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direction: direction/forward
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end: 934
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BBa_B0010
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top level: BBa_M30006
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component: BBa_B0010

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display id: range1758461
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orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1758471
display id: annotation1758471
version: 1
top level: BBa_M30006
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persistent identity: range1758471
display id: range1758471
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end: 1914
orientation: inline

BBa_E1010
type: SequenceAnnotation
persistent identity: annotation1758455
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version: 1
top level: BBa_M30006
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component: BBa_E1010

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BBa_B0012
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top level: BBa_M30006
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component: BBa_B0012

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persistent identity: range1758419
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direction: direction/forward
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start: 943
end: 983
orientation: inline

BBa_C0053
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display id: annotation1758401
version: 1
top level: BBa_M30006
location: https://synbiohub.org/public/igem/BBa_M30006/annotation1758401/range1758401/1
component: BBa_C0053

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persistent identity: range1758401
display id: range1758401
version: 1
direction: direction/forward
top level: BBa_M30006
start: 135
end: 821
orientation: inline

BBa_R0053
type: SequenceAnnotation
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display id: annotation1758438
version: 1
top level: BBa_M30006
location: https://synbiohub.org/public/igem/BBa_M30006/annotation1758438/range1758438/1
component: BBa_R0053

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persistent identity: range1758438
display id: range1758438
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direction: direction/forward
top level: BBa_M30006
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orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1758389
display id: annotation1758389
version: 1
top level: BBa_M30006
location: https://synbiohub.org/public/igem/BBa_M30006/annotation1758389/range1758389/1
component: BBa_B0034

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type: Range
persistent identity: range1758389
display id: range1758389
version: 1
direction: direction/forward
top level: BBa_M30006
start: 117
end: 128
orientation: inline

BBa_I13106
type: ComponentDefinition
persistent identity: BBa I13106
display id: BBa_I13106
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Low output control (tet QPI, Elowitz RBS)
creator: Barry Canton
created: 2004-07-20T11:00:00Z
modified: 2015-08-31T04:07:33Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_6_
m user id: 0
owner id: 135
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Lac promoter upstream
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Measurement
component: BBa_I13022
component: BBa_R0011
sequence annotation: BBa_I13022
sequence annotation: BBa_R0011
sequence: https://synbiohub.org/public/igem/BBa_I13106_sequence/1

BBa_I13022
type: Component
persistent identity: component2229310
display id: component2229310
version: 1
top level: BBa_I13106
access: public
definition: https://synbiohub.org/public/igem/BBa_I13022/1

BBa_R0011
type: Component
persistent identity: component2229279
display id: component2229279
version: 1
top level: BBa_I13106
access: public
definition: https://synbiohub.org/public/igem/BBa_R0011/1

BBa_I13022
type: SequenceAnnotation
persistent identity: annotation2229310
display id: annotation2229310
version: 1
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location: https://synbiohub.org/public/igem/BBa_I13106/annotation2229310/range2229310/1
component: BBa_I13022

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type: Range
persistent identity: range2229310
display id: range2229310
version: 1
direction: direction/reverse
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start: 64
end: 1851
orientation: inline

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation2229279
display id: annotation2229279
version: 1
top level: BBa_I13106
location: https://synbiohub.org/public/igem/BBa_I13106/annotation2229279/range2229279/1
component: BBa_R0011

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type: Range
persistent identity: range2229279
display id: range2229279
version: 1
direction: direction/forward
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start: 1
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orientation: inline

BBa_I13532
type: ComponentDefinition
persistent identity: BBa I13532
display id: BBa_I13532
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Screening Plasmid 2.4
creator: Josh Michener
created: 2005-07-28T11:00:00Z
modified: 2015-08-31T04:07:35Z
discontinued: true
dominant: false
group u list: _11_
m user id: 0
owner id: 253
owning group id: 6
sample status: Discontinued
status: status/Deleted
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Measurement
component: BBa_E1010
component: BBa_B0034
component: BBa_I13456
component: BBa_E0040
component: BBa_B0012
component: BBa_I0500
component: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_B0010
sequence annotation: BBa_I13456
sequence annotation: BBa_I0500
sequence annotation: BBa_E1010
sequence annotation: BBa_B0034
sequence annotation: BBa_E0040
sequence: https://synbiohub.org/public/igem/BBa_I13532_sequence/1

BBa_E1010
type: Component
persistent identity: component1598020
display id: component1598020
version: 1
top level: BBa_I13532
access: public
definition: https://synbiohub.org/public/igem/BBa_E1010/1

BBa_B0034
type: Component
persistent identity: component1598013
display id: component1598013
version: 1
top level: BBa_I13532
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0034
type: Component
persistent identity: component1597995
display id: component1597995
version: 1
top level: BBa_I13532
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_I13456
type: Component
persistent identity: component1598008
display id: component1598008
version: 1
top level: BBa_I13532
access: public
definition: https://synbiohub.org/public/igem/BBa_I13456/1

BBa_E0040
type: Component
persistent identity: component1597997
display id: component1597997
version: 1
top level: BBa_I13532
access: public
definition: https://synbiohub.org/public/igem/BBa_E0040/1

BBa_B0012
type: Component
persistent identity: component1598036
display id: component1598036
version: 1
top level: BBa_I13532
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_I0500
type: Component
persistent identity: component1597990
display id: component1597990
version: 1
top level: BBa_I13532
access: public
definition: https://synbiohub.org/public/igem/BBa_I0500/1

BBa_B0010
type: Component
persistent identity: component1598026
display id: component1598026
version: 1
top level: BBa_I13532
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1598036
display id: annotation1598036
version: 1
top level: BBa_I13532
location: https://synbiohub.org/public/igem/BBa_I13532/annotation1598036/range1598036/1
component: BBa_B0012

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type: Range
persistent identity: range1598036
display id: range1598036
version: 1
direction: direction/forward
top level: BBa_I13532
start: 2848
end: 2888
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1598026
display id: annotation1598026
version: 1
top level: BBa_I13532
location: https://synbiohub.org/public/igem/BBa_I13532/annotation1598026/range1598026/1
component: BBa_B0010

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persistent identity: range1598026
display id: range1598026
version: 1
direction: direction/forward
top level: BBa_I13532
start: 2760
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orientation: inline

BBa_I13456
type: SequenceAnnotation
persistent identity: annotation1598008
display id: annotation1598008
version: 1
top level: BBa_I13532
location: https://synbiohub.org/public/igem/BBa_I13532/annotation1598008/range1598008/1
component: BBa_I13456

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type: Range
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was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Low output control (c1 QPI)
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modified: 2015-08-31T04:07:33Z
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description: RBS Test R0011.B0030.E0030.B0015
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bookmark: false
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description: RBS Test R0040.B0032.E0030.B0015 RBS Test
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experience: experience/None
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display id: BBa_M30010
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was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: -- No description --
creator: natalie kuldell
created: 2005-12-04T12:00:00Z
modified: 2015-05-08T01:13:59Z
discontinued: false
dominant: true
group u list: _45_1_
m user id: 0
owner id: 314
owning group id: 1
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Positively regulated, OmpR-controlled promoter from OmpC gene (R0082) directing transcription of lambda cI gene. The cI protein represses expression of mRFP (M30003). Constructed with standard assembly method except R0082 was isolated using VF and VR-directed PCR. Consequently, there may be silent mutations that were inadvertently introduced.
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was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Screening Plasmid 2.3
creator: Josh Michener
created: 2005-07-28T11:00:00Z
modified: 2015-08-31T04:07:35Z
discontinued: true
dominant: false
group u list: _11_
m user id: 0
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owning group id: 6
sample status: Discontinued
status: status/Deleted
bookmark: false
mutable description: -- No description --
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star: false
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modified: 2015-08-31T04:07:45Z
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modified: 2015-08-31T04:07:33Z
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mutable description: -- No description --
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type: DnaRegion
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modified: 2015-08-31T04:07:45Z
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type: ComponentDefinition
persistent identity: BBa M30007
display id: BBa_M30007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: -- No description --
creator: natalie kuldell
created: 2005-12-04T12:00:00Z
modified: 2015-05-08T01:13:59Z
discontinued: false
dominant: true
group u list: _45_1_
m user id: 0
owner id: 314
owning group id: 1
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Positively regulated, OmpR-controlled promoter from OmpC gene (R0082) directing transcription of P22 c2 gene. The P22 c2 protein represses expression of mRFP (M30002). Constructed with standard assembly method except R0082 was isolated using VF and VR-directed PCR. Consequently, there may be silent mutations that were inadvertently introduced. A second isolate from this construction was saved as M30008.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Measurement
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component: BBa_B0034
component: BBa_R0053
component: BBa_C0053
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component: BBa_R0082
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sequence annotation: BBa_B0010
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sequence annotation: BBa_E1010
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BBa_I7101
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persistent identity: BBa I7101
display id: BBa_I7101
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: R0040.B0032.E0040.B0015
creator: jcbraff
created: 2004-10-25T11:00:00Z
modified: 2015-08-31T04:07:45Z
discontinued: false
dominant: true
experience: experience/None
group u list: _11_1_
m user id: 0
owner id: 61
owning group id: 7
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: R0040.B0032.E0040.B0015, GFP expression device
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Measurement
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component: BBa_B0032
component: BBa_R0040
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sequence annotation: BBa_B0032
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access: public
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display id: component1683641
version: 1
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version: 1
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display id: annotation1683612
version: 1
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BBa_I0411
type: ComponentDefinition
persistent identity: BBa I0411
display id: BBa_I0411
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS Test R0040.B0033.E0030.B0015
creator: jcbraff
created: 2004-06-13T11:00:00Z
modified: 2015-08-31T04:07:28Z
discontinued: false
dominant: true
experience: experience/None
group u list: _11_1_
m user id: 0
owner id: 61
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: R0040.B0033.E0030.B0015 RBS Test
owned by: james
owned by: myers
star: false
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role: SO:0000804
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BBa_B0010
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BBa_R0040
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display id: component946228
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BBa_I13623
type: ComponentDefinition
persistent identity: BBa I13623
display id: BBa_I13623
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Cascaded Lac (medium) and Tet (weak) QPIs
creator: mit
created: 2004-07-19T11:00:00Z
modified: 2015-08-31T04:07:36Z
discontinued: false
dominant: false
experience: experience/None
group u list: _6_
m user id: 0
owner id: 101
owning group id: 7
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Measurement
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display id: component2228958
version: 1
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BBa_I13107
type: ComponentDefinition
persistent identity: BBa I13107
display id: BBa_I13107
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Low output control (lac QPI, Elowitz RBS)
creator: Barry Canton
created: 2004-07-20T11:00:00Z
modified: 2015-08-31T04:07:33Z
discontinued: false
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top level: BBa_I6019
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component: BBa_I0500

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persistent identity: range979576
display id: range979576
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end: 3353
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BBa_B0034
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persistent identity: annotation979581
display id: annotation979581
version: 1
top level: BBa_I6019
location: https://synbiohub.org/public/igem/BBa_I6019/annotation979581/range979581/1
component: BBa_B0034

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type: Range
persistent identity: range979581
display id: range979581
version: 1
direction: direction/forward
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start: 3362
end: 3373
orientation: inline

BBa_E0032
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persistent identity: annotation979652
display id: annotation979652
version: 1
top level: BBa_I6019
location: https://synbiohub.org/public/igem/BBa_I6019/annotation979652/range979652/1
component: BBa_E0032

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BBa_B0010
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persistent identity: annotation979660
display id: annotation979660
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location: https://synbiohub.org/public/igem/BBa_I6019/annotation979660/range979660/1
component: BBa_B0010

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version: 1
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start: 5061
end: 5140
orientation: inline

BBa_I0500
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persistent identity: annotation979536
display id: annotation979536
version: 1
top level: BBa_I6019
location: https://synbiohub.org/public/igem/BBa_I6019/annotation979536/range979536/1
component: BBa_I0500

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persistent identity: range979536
display id: range979536
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direction: direction/forward
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BBa_B0012
type: SequenceAnnotation
persistent identity: annotation979670
display id: annotation979670
version: 1
top level: BBa_I6019
location: https://synbiohub.org/public/igem/BBa_I6019/annotation979670/range979670/1
component: BBa_B0012

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display id: range979670
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BBa_R0052
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persistent identity: annotation979630
display id: annotation979630
version: 1
top level: BBa_I6019
location: https://synbiohub.org/public/igem/BBa_I6019/annotation979630/range979630/1
component: BBa_R0052

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display id: range979630
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BBa_B0010
type: SequenceAnnotation
persistent identity: annotation979598
display id: annotation979598
version: 1
top level: BBa_I6019
location: https://synbiohub.org/public/igem/BBa_I6019/annotation979598/range979598/1
component: BBa_B0010

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persistent identity: range979598
display id: range979598
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BBa_E0022
type: SequenceAnnotation
persistent identity: annotation979553
display id: annotation979553
version: 1
top level: BBa_I6019
location: https://synbiohub.org/public/igem/BBa_I6019/annotation979553/range979553/1
component: BBa_E0022

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end: 1998
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type: SequenceAnnotation
persistent identity: annotation979541
display id: annotation979541
version: 1
top level: BBa_I6019
location: https://synbiohub.org/public/igem/BBa_I6019/annotation979541/range979541/1
component: BBa_B0034

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persistent identity: range979541
display id: range979541
version: 1
direction: direction/forward
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start: 1219
end: 1230
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BBa_C0052
type: SequenceAnnotation
persistent identity: annotation979591
display id: annotation979591
version: 1
top level: BBa_I6019
location: https://synbiohub.org/public/igem/BBa_I6019/annotation979591/range979591/1
component: BBa_C0052

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type: Range
persistent identity: range979591
display id: range979591
version: 1
direction: direction/forward
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start: 3380
end: 4048
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BBa_B0012
type: SequenceAnnotation
persistent identity: annotation979571
display id: annotation979571
version: 1
top level: BBa_I6019
location: https://synbiohub.org/public/igem/BBa_I6019/annotation979571/range979571/1
component: BBa_B0012

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persistent identity: range979571
display id: range979571
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BBa_B0010
type: SequenceAnnotation
persistent identity: annotation979561
display id: annotation979561
version: 1
top level: BBa_I6019
location: https://synbiohub.org/public/igem/BBa_I6019/annotation979561/range979561/1
component: BBa_B0010

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type: Range
persistent identity: range979561
display id: range979561
version: 1
direction: direction/forward
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end: 2086
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation979640
display id: annotation979640
version: 1
top level: BBa_I6019
location: https://synbiohub.org/public/igem/BBa_I6019/annotation979640/range979640/1
component: BBa_B0034

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type: Range
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display id: range979640
version: 1
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start: 4273
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BBa_K389421
type: ComponentDefinition
persistent identity: BBa K389421
display id: BBa_K389421
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: VirA/G signaling system + sensitivity tuner + luciferase
creator: Jonas Aretz
created: 2010-10-10T11:00:00Z
modified: 2015-05-08T01:12:19Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _517_
m user id: 0
owner id: 6670
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part combines a complete VirA/G signaling system with a sensitivity tuner before a luciferase gene under the control of a vir promoter. The sensitivity tuner enhances a signal from a promoter so it enhances the readout luciferase.
mutable notes: sensitivity tuner to enhance the luciferase signal
mutable provenance: * luciferase from pGL4.10luc2 (Promega) * sensitivity tuner part, terminators and constitutive promoter from the partsregistry * ''virG'' was synthesized by Mr. Gene * ''virA'' and ''vir'' promoter from Agrobacterium tumefaciens C58, TI-plasmid
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K389017
component: BBa_K389401
sequence annotation: BBa_K389401
sequence annotation: BBa_K389017
sequence: https://synbiohub.org/public/igem/BBa_K389421_sequence/1

BBa_K389017
type: Component
persistent identity: component2313848
display id: component2313848
version: 1
top level: BBa_K389421
access: public
definition: https://synbiohub.org/public/igem/BBa_K389017/1

BBa_K389401
type: Component
persistent identity: component2313872
display id: component2313872
version: 1
top level: BBa_K389421
access: public
definition: https://synbiohub.org/public/igem/BBa_K389401/1

BBa_K389401
type: SequenceAnnotation
persistent identity: annotation2313872
display id: annotation2313872
version: 1
top level: BBa_K389421
location: https://synbiohub.org/public/igem/BBa_K389421/annotation2313872/range2313872/1
component: BBa_K389401

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type: Range
persistent identity: range2313872
display id: range2313872
version: 1
direction: direction/reverse
top level: BBa_K389421
start: 3708
end: 6594
orientation: inline

BBa_K389017
type: SequenceAnnotation
persistent identity: annotation2313848
display id: annotation2313848
version: 1
top level: BBa_K389421
location: https://synbiohub.org/public/igem/BBa_K389421/annotation2313848/range2313848/1
component: BBa_K389017

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type: Range
persistent identity: range2313848
display id: range2313848
version: 1
direction: direction/reverse
top level: BBa_K389421
start: 1
end: 3699
orientation: inline

InATst
type: ComponentDefinition
persistent identity: BBa J05503
display id: BBa_J05503
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Sensing Device A (cI) TESTER (Pr+GFP+Prm+RFP)
creator: ETH Zurich iGEM Team 2005
created: 2005-09-21T11:00:00Z
modified: 2015-08-31T04:08:15Z
discontinued: false
dominant: false
experience: experience/None
group u list: _34_
m user id: 0
owner id: 398
owning group id: 34
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: A debug version of the event sensing and processing system for the iGEM2005 ETH "Counter" project: its purpose is to regulate cI production dependent on an external event. It works somehow like an inverter: STATE 1: when IPTG is absent, the constitutively produced lacI protein will block the lacI+pL promoter so that no cI is produced. Thus downstream the Pr promoter will be constitutively active, producing GFP (green fluorescence), while Prm+ will have low or no activity (no/low RFP - red fluorescence). STATE 2: when IPTG is present, it will remove the lacI protein from the lacI+pL promoter and cI is produced. Thus downstream the Pr promoter will be repressed (no GFP, i.e. no green fluorescence) by cI, while Prm+ will be activated, producing RFP (red fluorescence).
mutable notes: For more details see Sensing Device and Event Processing Device documentation.
mutable provenance: Registry 7.05
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Measurement
component: BBa_B0034
component: BBa_R0011
component: BBa_B0012
component: BBa_B0010
component: BBa_E1010
component: BBa_C0051
component: BBa_E0040
component: BBa_C0012
component: BBa_R0040
component: BBa_R0051
component: BBa_I12007
sequence annotation: BBa_B0034
sequence annotation: BBa_I12007
sequence annotation: BBa_B0010
sequence annotation: BBa_E1010
sequence annotation: BBa_C0051
sequence annotation: BBa_R0040
sequence annotation: BBa_B0012
sequence annotation: BBa_C0012
sequence annotation: BBa_E0040
sequence annotation: BBa_R0011
sequence annotation: BBa_R0051
sequence: https://synbiohub.org/public/igem/BBa_J05503_sequence/1

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persistent identity: component1702561
display id: component1702561
version: 1
top level: InATst
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0034
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persistent identity: component1702519
display id: component1702519
version: 1
top level: InATst
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_R0011
type: Component
persistent identity: component1702461
display id: component1702461
version: 1
top level: InATst
access: public
definition: https://synbiohub.org/public/igem/BBa_R0011/1

BBa_B0012
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persistent identity: component1702584
display id: component1702584
version: 1
top level: InATst
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0010
type: Component
persistent identity: component1702526
display id: component1702526
version: 1
top level: InATst
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_E1010
type: Component
persistent identity: component1702568
display id: component1702568
version: 1
top level: InATst
access: public
definition: https://synbiohub.org/public/igem/BBa_E1010/1

BBa_B0012
type: Component
persistent identity: component1702447
display id: component1702447
version: 1
top level: InATst
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0010
type: Component
persistent identity: component1702437
display id: component1702437
version: 1
top level: InATst
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0010
type: Component
persistent identity: component1702486
display id: component1702486
version: 1
top level: InATst
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_C0051
type: Component
persistent identity: component1702480
display id: component1702480
version: 1
top level: InATst
access: public
definition: https://synbiohub.org/public/igem/BBa_C0051/1

BBa_E0040
type: Component
persistent identity: component1702521
display id: component1702521
version: 1
top level: InATst
access: public
definition: https://synbiohub.org/public/igem/BBa_E0040/1

BBa_B0034
type: Component
persistent identity: component1702470
display id: component1702470
version: 1
top level: InATst
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0034
type: Component
persistent identity: component1702420
display id: component1702420
version: 1
top level: InATst
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_C0012
type: Component
persistent identity: component1702430
display id: component1702430
version: 1
top level: InATst
access: public
definition: https://synbiohub.org/public/igem/BBa_C0012/1

BBa_R0040
type: Component
persistent identity: component1702412
display id: component1702412
version: 1
top level: InATst
access: public
definition: https://synbiohub.org/public/igem/BBa_R0040/1

BBa_B0012
type: Component
persistent identity: component1702496
display id: component1702496
version: 1
top level: InATst
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0010
type: Component
persistent identity: component1702574
display id: component1702574
version: 1
top level: InATst
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0012
type: Component
persistent identity: component1702536
display id: component1702536
version: 1
top level: InATst
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_R0051
type: Component
persistent identity: component1702511
display id: component1702511
version: 1
top level: InATst
access: public
definition: https://synbiohub.org/public/igem/BBa_R0051/1

BBa_I12007
type: Component
persistent identity: component1702556
display id: component1702556
version: 1
top level: InATst
access: public
definition: https://synbiohub.org/public/igem/BBa_I12007/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1702470
display id: annotation1702470
version: 1
top level: InATst
location: https://synbiohub.org/public/igem/BBa_J05503/annotation1702470/range1702470/1
component: BBa_B0034

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type: Range
persistent identity: range1702470
display id: range1702470
version: 1
direction: direction/forward
top level: InATst
start: 1442
end: 1453
orientation: inline

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type: SequenceAnnotation
persistent identity: annotation1702556
display id: annotation1702556
version: 1
top level: InATst
location: https://synbiohub.org/public/igem/BBa_J05503/annotation1702556/range1702556/1
component: BBa_I12007

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persistent identity: range1702556
display id: range1702556
version: 1
direction: direction/forward
top level: InATst
start: 3320
end: 3401
orientation: inline

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type: SequenceAnnotation
persistent identity: annotation1702437
display id: annotation1702437
version: 1
top level: InATst
location: https://synbiohub.org/public/igem/BBa_J05503/annotation1702437/range1702437/1
component: BBa_B0010

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type: Range
persistent identity: range1702437
display id: range1702437
version: 1
direction: direction/forward
top level: InATst
start: 1242
end: 1321
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1702519
display id: annotation1702519
version: 1
top level: InATst
location: https://synbiohub.org/public/igem/BBa_J05503/annotation1702519/range1702519/1
component: BBa_B0034

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type: Range
persistent identity: range1702519
display id: range1702519
version: 1
direction: direction/forward
top level: InATst
start: 2437
end: 2448
orientation: inline

BBa_E1010
type: SequenceAnnotation
persistent identity: annotation1702568
display id: annotation1702568
version: 1
top level: InATst
location: https://synbiohub.org/public/igem/BBa_J05503/annotation1702568/range1702568/1
component: BBa_E1010

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type: Range
persistent identity: range1702568
display id: range1702568
version: 1
direction: direction/forward
top level: InATst
start: 3428
end: 4108
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1702486
display id: annotation1702486
version: 1
top level: InATst
location: https://synbiohub.org/public/igem/BBa_J05503/annotation1702486/range1702486/1
component: BBa_B0010

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type: Range
persistent identity: range1702486
display id: range1702486
version: 1
direction: direction/forward
top level: InATst
start: 2243
end: 2322
orientation: inline

BBa_C0051
type: SequenceAnnotation
persistent identity: annotation1702480
display id: annotation1702480
version: 1
top level: InATst
location: https://synbiohub.org/public/igem/BBa_J05503/annotation1702480/range1702480/1
component: BBa_C0051

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type: Range
persistent identity: range1702480
display id: range1702480
version: 1
direction: direction/forward
top level: InATst
start: 1460
end: 2209
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1702412
display id: annotation1702412
version: 1
top level: InATst
location: https://synbiohub.org/public/igem/BBa_J05503/annotation1702412/range1702412/1
component: BBa_R0040

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type: Range
persistent identity: range1702412
display id: range1702412
version: 1
direction: direction/forward
top level: InATst
start: 1
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orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1702420
display id: annotation1702420
version: 1
top level: InATst
location: https://synbiohub.org/public/igem/BBa_J05503/annotation1702420/range1702420/1
component: BBa_B0034

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type: Range
persistent identity: range1702420
display id: range1702420
version: 1
direction: direction/forward
top level: InATst
start: 63
end: 74
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1702584
display id: annotation1702584
version: 1
top level: InATst
location: https://synbiohub.org/public/igem/BBa_J05503/annotation1702584/range1702584/1
component: BBa_B0012

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type: Range
persistent identity: range1702584
display id: range1702584
version: 1
direction: direction/forward
top level: InATst
start: 4230
end: 4270
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1702447
display id: annotation1702447
version: 1
top level: InATst
location: https://synbiohub.org/public/igem/BBa_J05503/annotation1702447/range1702447/1
component: BBa_B0012

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type: Range
persistent identity: range1702447
display id: range1702447
version: 1
direction: direction/forward
top level: InATst
start: 1330
end: 1370
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1702496
display id: annotation1702496
version: 1
top level: InATst
location: https://synbiohub.org/public/igem/BBa_J05503/annotation1702496/range1702496/1
component: BBa_B0012

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type: Range
persistent identity: range1702496
display id: range1702496
version: 1
direction: direction/forward
top level: InATst
start: 2331
end: 2371
orientation: inline

BBa_C0012
type: SequenceAnnotation
persistent identity: annotation1702430
display id: annotation1702430
version: 1
top level: InATst
location: https://synbiohub.org/public/igem/BBa_J05503/annotation1702430/range1702430/1
component: BBa_C0012

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type: Range
persistent identity: range1702430
display id: range1702430
version: 1
direction: direction/forward
top level: InATst
start: 81
end: 1208
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation1702521
display id: annotation1702521
version: 1
top level: InATst
location: https://synbiohub.org/public/igem/BBa_J05503/annotation1702521/range1702521/1
component: BBa_E0040

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type: Range
persistent identity: range1702521
display id: range1702521
version: 1
direction: direction/forward
top level: InATst
start: 2455
end: 3174
orientation: inline

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation1702461
display id: annotation1702461
version: 1
top level: InATst
location: https://synbiohub.org/public/igem/BBa_J05503/annotation1702461/range1702461/1
component: BBa_R0011

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type: Range
persistent identity: range1702461
display id: range1702461
version: 1
direction: direction/forward
top level: InATst
start: 1379
end: 1432
orientation: inline

BBa_R0051
type: SequenceAnnotation
persistent identity: annotation1702511
display id: annotation1702511
version: 1
top level: InATst
location: https://synbiohub.org/public/igem/BBa_J05503/annotation1702511/range1702511/1
component: BBa_R0051

https://synbiohub.org/public/igem/BBa_J05503/annotation1702511/range1702511/1
type: Range
persistent identity: range1702511
display id: range1702511
version: 1
direction: direction/forward
top level: InATst
start: 2380
end: 2428
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1702561
display id: annotation1702561
version: 1
top level: InATst
location: https://synbiohub.org/public/igem/BBa_J05503/annotation1702561/range1702561/1
component: BBa_B0034

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type: Range
persistent identity: range1702561
display id: range1702561
version: 1
direction: direction/forward
top level: InATst
start: 3410
end: 3421
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1702526
display id: annotation1702526
version: 1
top level: InATst
location: https://synbiohub.org/public/igem/BBa_J05503/annotation1702526/range1702526/1
component: BBa_B0010

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type: Range
persistent identity: range1702526
display id: range1702526
version: 1
direction: direction/forward
top level: InATst
start: 3183
end: 3262
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1702536
display id: annotation1702536
version: 1
top level: InATst
location: https://synbiohub.org/public/igem/BBa_J05503/annotation1702536/range1702536/1
component: BBa_B0012

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type: Range
persistent identity: range1702536
display id: range1702536
version: 1
direction: direction/forward
top level: InATst
start: 3271
end: 3311
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1702574
display id: annotation1702574
version: 1
top level: InATst
location: https://synbiohub.org/public/igem/BBa_J05503/annotation1702574/range1702574/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_J05503/annotation1702574/range1702574/1
type: Range
persistent identity: range1702574
display id: range1702574
version: 1
direction: direction/forward
top level: InATst
start: 4142
end: 4221
orientation: inline

BBa_I0420
type: ComponentDefinition
persistent identity: BBa I0420
display id: BBa_I0420
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS Test R0040.E0030.B0015
creator: jcbraff
created: 2004-06-13T11:00:00Z
modified: 2015-08-31T04:07:29Z
discontinued: false
dominant: true
experience: experience/None
group u list: _11_1_
m user id: 0
owner id: 61
owning group id: 7
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: R0040.E0030.B0015 RBS Test control
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Measurement
component: BBa_B0010
component: BBa_B0012
component: BBa_R0040
component: BBa_E0030
sequence annotation: BBa_E0030
sequence annotation: BBa_B0012
sequence annotation: BBa_R0040
sequence annotation: BBa_B0010
sequence: https://synbiohub.org/public/igem/BBa_I0420_sequence/1

BBa_B0010
type: Component
persistent identity: component943048
display id: component943048
version: 1
top level: BBa_I0420
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0012
type: Component
persistent identity: component943058
display id: component943058
version: 1
top level: BBa_I0420
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_R0040
type: Component
persistent identity: component943038
display id: component943038
version: 1
top level: BBa_I0420
access: public
definition: https://synbiohub.org/public/igem/BBa_R0040/1

BBa_E0030
type: Component
persistent identity: component943043
display id: component943043
version: 1
top level: BBa_I0420
access: public
definition: https://synbiohub.org/public/igem/BBa_E0030/1

BBa_E0030
type: SequenceAnnotation
persistent identity: annotation943043
display id: annotation943043
version: 1
top level: BBa_I0420
location: https://synbiohub.org/public/igem/BBa_I0420/annotation943043/range943043/1
component: BBa_E0030

https://synbiohub.org/public/igem/BBa_I0420/annotation943043/range943043/1
type: Range
persistent identity: range943043
display id: range943043
version: 1
direction: direction/forward
top level: BBa_I0420
start: 61
end: 783
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation943058
display id: annotation943058
version: 1
top level: BBa_I0420
location: https://synbiohub.org/public/igem/BBa_I0420/annotation943058/range943058/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I0420/annotation943058/range943058/1
type: Range
persistent identity: range943058
display id: range943058
version: 1
direction: direction/forward
top level: BBa_I0420
start: 880
end: 920
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation943038
display id: annotation943038
version: 1
top level: BBa_I0420
location: https://synbiohub.org/public/igem/BBa_I0420/annotation943038/range943038/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_I0420/annotation943038/range943038/1
type: Range
persistent identity: range943038
display id: range943038
version: 1
direction: direction/forward
top level: BBa_I0420
start: 1
end: 54
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation943048
display id: annotation943048
version: 1
top level: BBa_I0420
location: https://synbiohub.org/public/igem/BBa_I0420/annotation943048/range943048/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I0420/annotation943048/range943048/1
type: Range
persistent identity: range943048
display id: range943048
version: 1
direction: direction/forward
top level: BBa_I0420
start: 792
end: 871
orientation: inline

BBa_K1682013
type: ComponentDefinition
persistent identity: BBa K1682013
display id: BBa_K1682013
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PphoA - I13504
creator: iGEM15_HKUST-Rice
created: 2015-09-01T11:00:00Z
modified: 2015-09-18T09:17:22Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2100_
m user id: 8758
owner id: 26069
owning group id: 9
status: status/Planning
bookmark: false
mutable description: phoApis a phosphate-responsive promoter. In high concentration of phosphate, it will be repressed while in low phosphate concentration, it will be activated.
mutable notes: N/A
mutable provenance: phoAp is native in E. coli
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Measurement
component: BBa_I13504
component: BBa_K1682012
sequence annotation: BBa_K1682012
sequence annotation: BBa_I13504
sequence: https://synbiohub.org/public/igem/BBa_K1682013_sequence/1

BBa_I13504
type: Component
persistent identity: component2473580
display id: component2473580
version: 1
top level: BBa_K1682013
access: public
definition: https://synbiohub.org/public/igem/BBa_I13504/1

BBa_K1682012
type: Component
persistent identity: component2473570
display id: component2473570
version: 1
top level: BBa_K1682013
access: public
definition: https://synbiohub.org/public/igem/BBa_K1682012/1

BBa_K1682012
type: SequenceAnnotation
persistent identity: annotation2473570
display id: annotation2473570
version: 1
top level: BBa_K1682013
location: https://synbiohub.org/public/igem/BBa_K1682013/annotation2473570/range2473570/1
component: BBa_K1682012

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type: Range
persistent identity: range2473570
display id: range2473570
version: 1
direction: direction/reverse
top level: BBa_K1682013
start: 1
end: 85
orientation: inline

BBa_I13504
type: SequenceAnnotation
persistent identity: annotation2473580
display id: annotation2473580
version: 1
top level: BBa_K1682013
location: https://synbiohub.org/public/igem/BBa_K1682013/annotation2473580/range2473580/1
component: BBa_I13504

https://synbiohub.org/public/igem/BBa_K1682013/annotation2473580/range2473580/1
type: Range
persistent identity: range2473580
display id: range2473580
version: 1
direction: direction/reverse
top level: BBa_K1682013
start: 94
end: 968
orientation: inline

Pvgb+GFP
type: ComponentDefinition
persistent identity: BBa K1555003
display id: BBa_K1555003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: GFP induced by micro-aerobic promoter vgb
creator: Li Huiyu, Jiang Zhenxiong, Wang Kun
created: 2014-10-05T11:00:00Z
modified: 2015-05-08T01:10:53Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1940_
m user id: 0
owner id: 22109
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This part consists of promoter vgb, RBS and GFP coding sequence. Promoter vgb is induced under micro-aerobic condition. Activated by the promoter, GFP coding gene expresses and the florescence intensity could represent expression rate of GFP to some degrees, by which we can confirm the character of this promoter.
mutable notes: Which restriction sites should be choosed to promise the high efficiency of assembling. Whether the linked product have capability to measure the expression of promoter vgb.
mutable provenance: All subparts are obtained in 2014 Distribution. All of them are designed and assembled by students.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Measurement
component: BBa_E0840
component: BBa_K561001
sequence annotation: BBa_K561001
sequence annotation: BBa_E0840
sequence: https://synbiohub.org/public/igem/BBa_K1555003_sequence/1

BBa_E0840
type: Component
persistent identity: component2397622
display id: component2397622
version: 1
top level: Pvgb+GFP
access: public
definition: https://synbiohub.org/public/igem/BBa_E0840/1

BBa_K561001
type: Component
persistent identity: component2397611
display id: component2397611
version: 1
top level: Pvgb+GFP
access: public
definition: https://synbiohub.org/public/igem/BBa_K561001/1

BBa_K561001
type: SequenceAnnotation
persistent identity: annotation2397611
display id: annotation2397611
version: 1
top level: Pvgb+GFP
location: https://synbiohub.org/public/igem/BBa_K1555003/annotation2397611/range2397611/1
component: BBa_K561001

https://synbiohub.org/public/igem/BBa_K1555003/annotation2397611/range2397611/1
type: Range
persistent identity: range2397611
display id: range2397611
version: 1
direction: direction/reverse
top level: Pvgb+GFP
start: 1
end: 135
orientation: inline

BBa_E0840
type: SequenceAnnotation
persistent identity: annotation2397622
display id: annotation2397622
version: 1
top level: Pvgb+GFP
location: https://synbiohub.org/public/igem/BBa_K1555003/annotation2397622/range2397622/1
component: BBa_E0840

https://synbiohub.org/public/igem/BBa_K1555003/annotation2397622/range2397622/1
type: Range
persistent identity: range2397622
display id: range2397622
version: 1
direction: direction/reverse
top level: Pvgb+GFP
start: 144
end: 1021
orientation: inline

BBa_M30011
type: ComponentDefinition
persistent identity: BBa M30011
display id: BBa_M30011
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: OmpR controlled mRFP
creator: natalie kuldell
created: 2005-12-14T12:00:00Z
modified: 2015-09-17T06:41:23Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _45_1_
m user id: 25546
owner id: 314
owning group id: 1
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Positively regulated, OmpR-controlled promoter from OmpC gene (R0082) directing transcription of mRFP (I13507). Constructed with standard assembly method except R0082 was isolated using VF and VR-directed PCR. Consequently, there may be silent mutations that were inadvertently introduced. An alternative clone is M30012. Cells bearing this construct are pale pink.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Measurement
component: BBa_R0082
component: BBa_B0034
component: BBa_E1010
component: BBa_B0010
component: BBa_B0012
sequence annotation: BBa_B0012
sequence annotation: BBa_B0010
sequence annotation: BBa_E1010
sequence annotation: BBa_R0082
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_M30011_sequence/1

BBa_R0082
type: Component
persistent identity: component1768968
display id: component1768968
version: 1
top level: BBa_M30011
access: public
definition: https://synbiohub.org/public/igem/BBa_R0082/1

BBa_B0034
type: Component
persistent identity: component1768973
display id: component1768973
version: 1
top level: BBa_M30011
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_E1010
type: Component
persistent identity: component1768980
display id: component1768980
version: 1
top level: BBa_M30011
access: public
definition: https://synbiohub.org/public/igem/BBa_E1010/1

BBa_B0010
type: Component
persistent identity: component1768986
display id: component1768986
version: 1
top level: BBa_M30011
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0012
type: Component
persistent identity: component1768996
display id: component1768996
version: 1
top level: BBa_M30011
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1768996
display id: annotation1768996
version: 1
top level: BBa_M30011
location: https://synbiohub.org/public/igem/BBa_M30011/annotation1768996/range1768996/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_M30011/annotation1768996/range1768996/1
type: Range
persistent identity: range1768996
display id: range1768996
version: 1
direction: direction/forward
top level: BBa_M30011
start: 937
end: 977
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1768986
display id: annotation1768986
version: 1
top level: BBa_M30011
location: https://synbiohub.org/public/igem/BBa_M30011/annotation1768986/range1768986/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_M30011/annotation1768986/range1768986/1
type: Range
persistent identity: range1768986
display id: range1768986
version: 1
direction: direction/forward
top level: BBa_M30011
start: 849
end: 928
orientation: inline

BBa_E1010
type: SequenceAnnotation
persistent identity: annotation1768980
display id: annotation1768980
version: 1
top level: BBa_M30011
location: https://synbiohub.org/public/igem/BBa_M30011/annotation1768980/range1768980/1
component: BBa_E1010

https://synbiohub.org/public/igem/BBa_M30011/annotation1768980/range1768980/1
type: Range
persistent identity: range1768980
display id: range1768980
version: 1
direction: direction/forward
top level: BBa_M30011
start: 135
end: 815
orientation: inline

BBa_R0082
type: SequenceAnnotation
persistent identity: annotation1768968
display id: annotation1768968
version: 1
top level: BBa_M30011
location: https://synbiohub.org/public/igem/BBa_M30011/annotation1768968/range1768968/1
component: BBa_R0082

https://synbiohub.org/public/igem/BBa_M30011/annotation1768968/range1768968/1
type: Range
persistent identity: range1768968
display id: range1768968
version: 1
direction: direction/forward
top level: BBa_M30011
start: 1
end: 108
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1768973
display id: annotation1768973
version: 1
top level: BBa_M30011
location: https://synbiohub.org/public/igem/BBa_M30011/annotation1768973/range1768973/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_M30011/annotation1768973/range1768973/1
type: Range
persistent identity: range1768973
display id: range1768973
version: 1
direction: direction/forward
top level: BBa_M30011
start: 117
end: 128
orientation: inline

BBa_I13010
type: ComponentDefinition
persistent identity: BBa I13010
display id: BBa_I13010
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS Test R0011.B0033. E0130
creator: jasonk
created: 2004-06-30T11:00:00Z
modified: 2015-08-31T04:07:32Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_6_
m user id: 0
owner id: 135
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Parts I13007-I130014 are RBS test constructs for RBS characterization. Each consists of Lac or Tet promoter, one of the four RBS's B0030-B0033, a YFP coding protein, and B0015 terminator.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Measurement
component: BBa_B0033
component: BBa_E0130
component: BBa_R0011
sequence annotation: BBa_B0033
sequence annotation: BBa_R0011
sequence annotation: BBa_E0130
sequence: https://synbiohub.org/public/igem/BBa_I13010_sequence/1

BBa_B0033
type: Component
persistent identity: component2221453
display id: component2221453
version: 1
top level: BBa_I13010
access: public
definition: https://synbiohub.org/public/igem/BBa_B0033/1

BBa_E0130
type: Component
persistent identity: component2221462
display id: component2221462
version: 1
top level: BBa_I13010
access: public
definition: https://synbiohub.org/public/igem/BBa_E0130/1

BBa_R0011
type: Component
persistent identity: component2221447
display id: component2221447
version: 1
top level: BBa_I13010
access: public
definition: https://synbiohub.org/public/igem/BBa_R0011/1

BBa_B0033
type: SequenceAnnotation
persistent identity: annotation2221453
display id: annotation2221453
version: 1
top level: BBa_I13010
location: https://synbiohub.org/public/igem/BBa_I13010/annotation2221453/range2221453/1
component: BBa_B0033

https://synbiohub.org/public/igem/BBa_I13010/annotation2221453/range2221453/1
type: Range
persistent identity: range2221453
display id: range2221453
version: 1
direction: direction/forward
top level: BBa_I13010
start: 64
end: 74
orientation: inline

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation2221447
display id: annotation2221447
version: 1
top level: BBa_I13010
location: https://synbiohub.org/public/igem/BBa_I13010/annotation2221447/range2221447/1
component: BBa_R0011

https://synbiohub.org/public/igem/BBa_I13010/annotation2221447/range2221447/1
type: Range
persistent identity: range2221447
display id: range2221447
version: 1
direction: direction/forward
top level: BBa_I13010
start: 1
end: 54
orientation: inline

BBa_E0130
type: SequenceAnnotation
persistent identity: annotation2221462
display id: annotation2221462
version: 1
top level: BBa_I13010
location: https://synbiohub.org/public/igem/BBa_I13010/annotation2221462/range2221462/1
component: BBa_E0130

https://synbiohub.org/public/igem/BBa_I13010/annotation2221462/range2221462/1
type: Range
persistent identity: range2221462
display id: range2221462
version: 1
direction: direction/reverse
top level: BBa_I13010
start: 81
end: 940
orientation: inline

BBa_I13614
type: ComponentDefinition
persistent identity: BBa I13614
display id: BBa_I13614
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Cascaded Tet (medium) and Lac (medium) QPIs
creator: mit
created: 2004-07-19T11:00:00Z
modified: 2015-08-31T04:07:36Z
discontinued: false
dominant: false
experience: experience/None
group u list: _6_
m user id: 0
owner id: 101
owning group id: 7
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Measurement
component: BBa_Q01400
component: BBa_Q01121
sequence annotation: BBa_Q01121
sequence annotation: BBa_Q01400
sequence: https://synbiohub.org/public/igem/BBa_I13614_sequence/1

BBa_Q01400
type: Component
persistent identity: component2228826
display id: component2228826
version: 1
top level: BBa_I13614
access: public
definition: https://synbiohub.org/public/igem/BBa_Q01400/1

BBa_Q01121
type: Component
persistent identity: component2228845
display id: component2228845
version: 1
top level: BBa_I13614
access: public
definition: https://synbiohub.org/public/igem/BBa_Q01121/1

BBa_Q01121
type: SequenceAnnotation
persistent identity: annotation2228845
display id: annotation2228845
version: 1
top level: BBa_I13614
location: https://synbiohub.org/public/igem/BBa_I13614/annotation2228845/range2228845/1
component: BBa_Q01121

https://synbiohub.org/public/igem/BBa_I13614/annotation2228845/range2228845/1
type: Range
persistent identity: range2228845
display id: range2228845
version: 1
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BBa_I0419
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display id: BBa_I0419
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was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS Test R0011.B0032.E0030.B0015
creator: jcbraff
created: 2004-06-13T11:00:00Z
modified: 2015-08-31T04:07:28Z
discontinued: false
dominant: true
experience: experience/None
group u list: _11_1_
m user id: 0
owner id: 61
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: R0011.B0032.E0030.B0015 RBS Test
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Measurement
component: BBa_B0015
component: BBa_R0011
component: BBa_B0032
component: BBa_E0030
sequence annotation: BBa_B0032
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sequence annotation: BBa_E0030
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BBa_B0015
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BBa_R0011
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definition: https://synbiohub.org/public/igem/BBa_R0011/1

BBa_B0032
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version: 1
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BBa_E0030
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BBa_B0032
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BBa_B0015
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BBa_R0011
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BBa_I13647
type: ComponentDefinition
persistent identity: BBa I13647
display id: BBa_I13647
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Cascaded Lac (elowitz) and Tet (medium) QPIs with double terminator as input
creator: mit
created: 2004-07-19T11:00:00Z
modified: 2015-08-31T04:07:36Z
discontinued: false
dominant: false
experience: experience/None
group u list: _6_
m user id: 0
owner id: 101
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Measurement
component: BBa_Q01400
component: BBa_B0015
component: BBa_Q04121
sequence annotation: BBa_Q04121
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sequence: https://synbiohub.org/public/igem/BBa_I13647_sequence/1

BBa_Q01400
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display id: component2232468
version: 1
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access: public
definition: https://synbiohub.org/public/igem/BBa_Q01400/1

BBa_B0015
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version: 1
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definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_Q04121
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BBa_B0015
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type: ComponentDefinition
persistent identity: BBa I13115
display id: BBa_I13115
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: High output control (tet QPI)
creator: Barry Canton
created: 2004-07-20T11:00:00Z
modified: 2015-08-31T04:07:33Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_6_
m user id: 0
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owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: terminator upstream, no promoter
owned by: james
owned by: myers
star: false
type: DnaRegion
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component: BBa_B0012
component: BBa_R0040
component: BBa_B0010
component: BBa_B0034
component: BBa_E0030
component: BBa_C0040
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sequence: https://synbiohub.org/public/igem/BBa_I13115_sequence/1

BBa_B0012
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BBa_B0012
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BBa_R0040
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BBa_B0010
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definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0010
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BBa_B0034
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BBa_B0012
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BBa_B0010
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BBa_E0030
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BBa_B0034
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BBa_C0040
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BBa_B0010
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BBa_B0012
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BBa_B0034
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BBa_B0010
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BBa_B0034
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BBa_B0012
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display id: range972142
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BBa_R0040
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version: 1
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component: BBa_R0040

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display id: range972127
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BBa_I12243
type: ComponentDefinition
persistent identity: BBa I12243
display id: BBa_I12243
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Test of AraC protein generator
creator: rwald
created: 2004-08-31T11:00:00Z
modified: 2015-08-31T04:07:32Z
discontinued: false
dominant: false
experience: experience/None
group u list: _3_
m user id: 0
owner id: 120
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This component tests the I12222 TetR protein generator. It uses the R0080 promoter region for the test.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Measurement
component: BBa_B0010
component: BBa_B0012
component: BBa_B0032
component: BBa_B0034
component: BBa_R0053
component: BBa_E0022
component: BBa_I12205
component: BBa_R0080
sequence annotation: BBa_B0012
sequence annotation: BBa_E0022
sequence annotation: BBa_B0034
sequence annotation: BBa_R0053
sequence annotation: BBa_B0010
sequence annotation: BBa_B0032
sequence annotation: BBa_R0080
sequence annotation: BBa_I12205
sequence: https://synbiohub.org/public/igem/BBa_I12243_sequence/1

BBa_B0010
type: Component
persistent identity: component1063453
display id: component1063453
version: 1
top level: BBa_I12243
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0012
type: Component
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display id: component1063521
version: 1
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access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0032
type: Component
persistent identity: component1063439
display id: component1063439
version: 1
top level: BBa_I12243
access: public
definition: https://synbiohub.org/public/igem/BBa_B0032/1

BBa_B0010
type: Component
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display id: component1063511
version: 1
top level: BBa_I12243
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0012
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display id: component1063463
version: 1
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access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0034
type: Component
persistent identity: component1063491
display id: component1063491
version: 1
top level: BBa_I12243
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_R0053
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display id: component1063426
version: 1
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access: public
definition: https://synbiohub.org/public/igem/BBa_R0053/1

BBa_E0022
type: Component
persistent identity: component1063503
display id: component1063503
version: 1
top level: BBa_I12243
access: public
definition: https://synbiohub.org/public/igem/BBa_E0022/1

BBa_I12205
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display id: component1063447
version: 1
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access: public
definition: https://synbiohub.org/public/igem/BBa_I12205/1

BBa_R0080
type: Component
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display id: component1063486
version: 1
top level: BBa_I12243
access: public
definition: https://synbiohub.org/public/igem/BBa_R0080/1

BBa_B0012
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orientation: inline

BBa_E0022
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display id: range1063503
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direction: direction/forward
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BBa_B0034
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BBa_R0053
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BBa_B0010
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persistent identity: range1063453
display id: range1063453
version: 1
direction: direction/forward
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end: 1076
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation1063439
display id: annotation1063439
version: 1
top level: BBa_I12243
location: https://synbiohub.org/public/igem/BBa_I12243/annotation1063439/range1063439/1
component: BBa_B0032

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type: Range
persistent identity: range1063439
display id: range1063439
version: 1
direction: direction/forward
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start: 63
end: 75
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1063521
display id: annotation1063521
version: 1
top level: BBa_I12243
location: https://synbiohub.org/public/igem/BBa_I12243/annotation1063521/range1063521/1
component: BBa_B0012

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type: Range
persistent identity: range1063521
display id: range1063521
version: 1
direction: direction/forward
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start: 2167
end: 2207
orientation: inline

BBa_R0080
type: SequenceAnnotation
persistent identity: annotation1063486
display id: annotation1063486
version: 1
top level: BBa_I12243
location: https://synbiohub.org/public/igem/BBa_I12243/annotation1063486/range1063486/1
component: BBa_R0080

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persistent identity: range1063486
display id: range1063486
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direction: direction/forward
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start: 1134
end: 1282
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1063511
display id: annotation1063511
version: 1
top level: BBa_I12243
location: https://synbiohub.org/public/igem/BBa_I12243/annotation1063511/range1063511/1
component: BBa_B0010

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persistent identity: range1063511
display id: range1063511
version: 1
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start: 2079
end: 2158
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BBa_I12205
type: SequenceAnnotation
persistent identity: annotation1063447
display id: annotation1063447
version: 1
top level: BBa_I12243
location: https://synbiohub.org/public/igem/BBa_I12243/annotation1063447/range1063447/1
component: BBa_I12205

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type: Range
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display id: range1063447
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end: 963
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BBa_K1379007
type: ComponentDefinition
persistent identity: BBa K1379007
display id: BBa_K1379007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: σx Generator + PcomFA-E0240
creator: Ng Siu Wang Edward, Jason Jang, Joo Min Jung
created: 2014-09-22T11:00:00Z
modified: 2015-05-08T01:10:12Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1756_
m user id: 0
owner id: 21235
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: SigmaX generator, SigmaX-inducible Phelicase encoding GFP
mutable notes: N/A
mutable provenance: Both comX gene and Phelicase are found in genomic sequence of Streptococcus pneumoniae NCTC7465 strain, while the RBS+GFP+DTT, downstream of PCelA, is BBa_E0240. The promoter encoding comX is BBa_K880005 and the double terminator for it is BBa_B0015
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Measurement
component: BBa_K1379006
component: BBa_K1379001
component: BBa_E0240
sequence annotation: BBa_K1379006
sequence annotation: BBa_E0240
sequence annotation: BBa_K1379001
sequence: https://synbiohub.org/public/igem/BBa_K1379007_sequence/1

BBa_K1379006
type: Component
persistent identity: component2421710
display id: component2421710
version: 1
top level: BBa_K1379007
access: public
definition: https://synbiohub.org/public/igem/BBa_K1379006/1

BBa_K1379001
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persistent identity: component2421713
display id: component2421713
version: 1
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access: public
definition: https://synbiohub.org/public/igem/BBa_K1379001/1

BBa_E0240
type: Component
persistent identity: component2421724
display id: component2421724
version: 1
top level: BBa_K1379007
access: public
definition: https://synbiohub.org/public/igem/BBa_E0240/1

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persistent identity: annotation2421710
display id: annotation2421710
version: 1
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component: BBa_K1379006

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direction: direction/reverse
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BBa_E0240
type: SequenceAnnotation
persistent identity: annotation2421724
display id: annotation2421724
version: 1
top level: BBa_K1379007
location: https://synbiohub.org/public/igem/BBa_K1379007/annotation2421724/range2421724/1
component: BBa_E0240

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type: Range
persistent identity: range2421724
display id: range2421724
version: 1
direction: direction/reverse
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type: SequenceAnnotation
persistent identity: annotation2421713
display id: annotation2421713
version: 1
top level: BBa_K1379007
location: https://synbiohub.org/public/igem/BBa_K1379007/annotation2421713/range2421713/1
component: BBa_K1379001

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type: Range
persistent identity: range2421713
display id: range2421713
version: 1
direction: direction/reverse
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start: 720
end: 879
orientation: inline

BBa_I13626
type: ComponentDefinition
persistent identity: BBa I13626
display id: BBa_I13626
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Cascaded Lac (elowitz) and Tet (weak) QPIs
creator: mit
created: 2004-07-19T11:00:00Z
modified: 2015-08-31T04:07:36Z
discontinued: false
dominant: false
experience: experience/None
group u list: _6_
m user id: 0
owner id: 101
owning group id: 7
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Measurement
component: BBa_Q04121
component: BBa_Q03400
sequence annotation: BBa_Q03400
sequence annotation: BBa_Q04121
sequence: https://synbiohub.org/public/igem/BBa_I13626_sequence/1

BBa_Q04121
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persistent identity: component2229014
display id: component2229014
version: 1
top level: BBa_I13626
access: public
definition: https://synbiohub.org/public/igem/BBa_Q04121/1

BBa_Q03400
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persistent identity: component2229033
display id: component2229033
version: 1
top level: BBa_I13626
access: public
definition: https://synbiohub.org/public/igem/BBa_Q03400/1

BBa_Q03400
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persistent identity: annotation2229033
display id: annotation2229033
version: 1
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type: Range
persistent identity: range2229033
display id: range2229033
version: 1
direction: direction/reverse
top level: BBa_I13626
start: 1380
end: 2280
orientation: inline

BBa_Q04121
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display id: annotation2229014
version: 1
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component: BBa_Q04121

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display id: range2229014
version: 1
direction: direction/reverse
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orientation: inline

BBa_I13631
type: ComponentDefinition
persistent identity: BBa I13631
display id: BBa_I13631
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Cascaded Tet (weak) and Lac (medium) QPIs with double terminator as input
creator: mit
created: 2004-07-19T11:00:00Z
modified: 2015-08-31T04:07:36Z
discontinued: false
dominant: false
experience: experience/None
group u list: _6_
m user id: 0
owner id: 101
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Measurement
component: BBa_Q03400
component: BBa_Q01121
component: BBa_B0015
sequence annotation: BBa_Q03400
sequence annotation: BBa_B0015
sequence annotation: BBa_Q01121
sequence: https://synbiohub.org/public/igem/BBa_I13631_sequence/1

BBa_Q03400
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persistent identity: component2232228
display id: component2232228
version: 1
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access: public
definition: https://synbiohub.org/public/igem/BBa_Q03400/1

BBa_Q01121
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persistent identity: component2232247
display id: component2232247
version: 1
top level: BBa_I13631
access: public
definition: https://synbiohub.org/public/igem/BBa_Q01121/1

BBa_B0015
type: Component
persistent identity: component2232209
display id: component2232209
version: 1
top level: BBa_I13631
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_Q03400
type: SequenceAnnotation
persistent identity: annotation2232228
display id: annotation2232228
version: 1
top level: BBa_I13631
location: https://synbiohub.org/public/igem/BBa_I13631/annotation2232228/range2232228/1
component: BBa_Q03400

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type: Range
persistent identity: range2232228
display id: range2232228
version: 1
direction: direction/reverse
top level: BBa_I13631
start: 138
end: 1038
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2232209
display id: annotation2232209
version: 1
top level: BBa_I13631
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component: BBa_B0015

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type: Range
persistent identity: range2232209
display id: range2232209
version: 1
direction: direction/reverse
top level: BBa_I13631
start: 1
end: 129
orientation: inline

BBa_Q01121
type: SequenceAnnotation
persistent identity: annotation2232247
display id: annotation2232247
version: 1
top level: BBa_I13631
location: https://synbiohub.org/public/igem/BBa_I13631/annotation2232247/range2232247/1
component: BBa_Q01121

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type: Range
persistent identity: range2232247
display id: range2232247
version: 1
direction: direction/reverse
top level: BBa_I13631
start: 1047
end: 2419
orientation: inline

BBa_I6319
type: ComponentDefinition
persistent identity: BBa I6319
display id: BBa_I6319
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: QPI Curve Test I0500.E0420.I0500.Q04520.E0430
creator: cconboy
created: 2004-05-09T11:00:00Z
modified: 2015-08-31T04:07:44Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_1_
m user id: 0
owner id: 60
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Measurement
component: BBa_R0052
component: BBa_I0500
component: BBa_B0034
component: BBa_B0012
component: BBa_C0052
component: BBa_B0010
component: BBa_E0030
component: BBa_E0020
sequence annotation: BBa_B0012
sequence annotation: BBa_E0030
sequence annotation: BBa_R0052
sequence annotation: BBa_B0010
sequence annotation: BBa_I0500
sequence annotation: BBa_B0034
sequence annotation: BBa_E0020
sequence annotation: BBa_C0052
sequence: https://synbiohub.org/public/igem/BBa_I6319_sequence/1

BBa_R0052
type: Component
persistent identity: component980529
display id: component980529
version: 1
top level: BBa_I6319
access: public
definition: https://synbiohub.org/public/igem/BBa_R0052/1

BBa_I0500
type: Component
persistent identity: component980448
display id: component980448
version: 1
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access: public
definition: https://synbiohub.org/public/igem/BBa_I0500/1

BBa_B0034
type: Component
persistent identity: component980480
display id: component980480
version: 1
top level: BBa_I6319
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0012
type: Component
persistent identity: component980556
display id: component980556
version: 1
top level: BBa_I6319
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_C0052
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persistent identity: component980490
display id: component980490
version: 1
top level: BBa_I6319
access: public
definition: https://synbiohub.org/public/igem/BBa_C0052/1

BBa_B0010
type: Component
persistent identity: component980460
display id: component980460
version: 1
top level: BBa_I6319
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_E0030
type: Component
persistent identity: component980541
display id: component980541
version: 1
top level: BBa_I6319
access: public
definition: https://synbiohub.org/public/igem/BBa_E0030/1

BBa_B0034
type: Component
persistent identity: component980539
display id: component980539
version: 1
top level: BBa_I6319
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_I0500
type: Component
persistent identity: component980475
display id: component980475
version: 1
top level: BBa_I6319
access: public
definition: https://synbiohub.org/public/igem/BBa_I0500/1

BBa_B0010
type: Component
persistent identity: component980546
display id: component980546
version: 1
top level: BBa_I6319
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0012
type: Component
persistent identity: component980507
display id: component980507
version: 1
top level: BBa_I6319
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_E0020
type: Component
persistent identity: component980455
display id: component980455
version: 1
top level: BBa_I6319
access: public
definition: https://synbiohub.org/public/igem/BBa_E0020/1

BBa_B0010
type: Component
persistent identity: component980497
display id: component980497
version: 1
top level: BBa_I6319
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0012
type: Component
persistent identity: component980470
display id: component980470
version: 1
top level: BBa_I6319
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0034
type: Component
persistent identity: component980453
display id: component980453
version: 1
top level: BBa_I6319
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation980556
display id: annotation980556
version: 1
top level: BBa_I6319
location: https://synbiohub.org/public/igem/BBa_I6319/annotation980556/range980556/1
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type: Range
persistent identity: range980556
display id: range980556
version: 1
direction: direction/forward
top level: BBa_I6319
start: 5071
end: 5111
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation980470
display id: annotation980470
version: 1
top level: BBa_I6319
location: https://synbiohub.org/public/igem/BBa_I6319/annotation980470/range980470/1
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persistent identity: range980470
display id: range980470
version: 1
direction: direction/forward
top level: BBa_I6319
start: 2056
end: 2096
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation980507
display id: annotation980507
version: 1
top level: BBa_I6319
location: https://synbiohub.org/public/igem/BBa_I6319/annotation980507/range980507/1
component: BBa_B0012

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persistent identity: range980507
display id: range980507
version: 1
direction: direction/forward
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start: 4131
end: 4171
orientation: inline

BBa_E0030
type: SequenceAnnotation
persistent identity: annotation980541
display id: annotation980541
version: 1
top level: BBa_I6319
location: https://synbiohub.org/public/igem/BBa_I6319/annotation980541/range980541/1
component: BBa_E0030

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type: Range
persistent identity: range980541
display id: range980541
version: 1
direction: direction/forward
top level: BBa_I6319
start: 4252
end: 4974
orientation: inline

BBa_R0052
type: SequenceAnnotation
persistent identity: annotation980529
display id: annotation980529
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persistent identity: range980529
display id: range980529
version: 1
direction: direction/forward
top level: BBa_I6319
start: 4180
end: 4225
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation980460
display id: annotation980460
version: 1
top level: BBa_I6319
location: https://synbiohub.org/public/igem/BBa_I6319/annotation980460/range980460/1
component: BBa_B0010

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type: Range
persistent identity: range980460
display id: range980460
version: 1
direction: direction/forward
top level: BBa_I6319
start: 1968
end: 2047
orientation: inline

BBa_I0500
type: SequenceAnnotation
persistent identity: annotation980475
display id: annotation980475
version: 1
top level: BBa_I6319
location: https://synbiohub.org/public/igem/BBa_I6319/annotation980475/range980475/1
component: BBa_I0500

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persistent identity: range980475
display id: range980475
version: 1
direction: direction/forward
top level: BBa_I6319
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end: 3314
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation980539
display id: annotation980539
version: 1
top level: BBa_I6319
location: https://synbiohub.org/public/igem/BBa_I6319/annotation980539/range980539/1
component: BBa_B0034

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type: Range
persistent identity: range980539
display id: range980539
version: 1
direction: direction/forward
top level: BBa_I6319
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end: 4245
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation980480
display id: annotation980480
version: 1
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location: https://synbiohub.org/public/igem/BBa_I6319/annotation980480/range980480/1
component: BBa_B0034

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type: Range
persistent identity: range980480
display id: range980480
version: 1
direction: direction/forward
top level: BBa_I6319
start: 3323
end: 3334
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation980546
display id: annotation980546
version: 1
top level: BBa_I6319
location: https://synbiohub.org/public/igem/BBa_I6319/annotation980546/range980546/1
component: BBa_B0010

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type: Range
persistent identity: range980546
display id: range980546
version: 1
direction: direction/forward
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start: 4983
end: 5062
orientation: inline

BBa_E0020
type: SequenceAnnotation
persistent identity: annotation980455
display id: annotation980455
version: 1
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location: https://synbiohub.org/public/igem/BBa_I6319/annotation980455/range980455/1
component: BBa_E0020

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type: Range
persistent identity: range980455
display id: range980455
version: 1
direction: direction/forward
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start: 1237
end: 1959
orientation: inline

BBa_I0500
type: SequenceAnnotation
persistent identity: annotation980448
display id: annotation980448
version: 1
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type: Range
persistent identity: range980448
display id: range980448
version: 1
direction: direction/forward
top level: BBa_I6319
start: 1
end: 1210
orientation: inline

BBa_C0052
type: SequenceAnnotation
persistent identity: annotation980490
display id: annotation980490
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component: BBa_C0052

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display id: range980490
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end: 4122
orientation: inline

BBa_B0034
type: SequenceAnnotation
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display id: annotation980453
version: 1
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component: BBa_B0034

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persistent identity: range980453
display id: range980453
version: 1
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end: 1230
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BBa_I13514
type: ComponentDefinition
persistent identity: BBa I13514
display id: BBa_I13514
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Screening Plasmid 3
creator: jkm
created: 2005-05-30T11:00:00Z
modified: 2015-08-31T04:07:34Z
discontinued: false
dominant: true
experience: experience/None
group u list: _11_
m user id: 0
owner id: 253
owning group id: 6
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: The third screening plasmid. Same as the second except with the flurophores switched. The ends should be capped with I13450.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Measurement
component: BBa_I0500
component: BBa_B0034
component: BBa_E0040
component: BBa_B0010
component: BBa_E1010
component: BBa_B0012
sequence annotation: BBa_I0500
sequence annotation: BBa_B0010
sequence annotation: BBa_B0034
sequence annotation: BBa_E1010
sequence annotation: BBa_E0040
sequence annotation: BBa_B0012
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BBa_I0500
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display id: component1524851
version: 1
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access: public
definition: https://synbiohub.org/public/igem/BBa_I0500/1

BBa_B0034
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access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0034
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access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_E0040
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persistent identity: component1524858
display id: component1524858
version: 1
top level: BBa_I13514
access: public
definition: https://synbiohub.org/public/igem/BBa_E0040/1

BBa_B0010
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definition: https://synbiohub.org/public/igem/BBa_B0010/1

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display id: component1524870
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access: public
definition: https://synbiohub.org/public/igem/BBa_E1010/1

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version: 1
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access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

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display id: annotation1524851
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component: BBa_I0500

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BBa_B0034
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top level: BBa_I13514
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component: BBa_B0034

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orientation: inline

BBa_E1010
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display id: annotation1524870
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top level: BBa_I13514
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component: BBa_E1010

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BBa_B0034
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persistent identity: annotation1524856
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version: 1
top level: BBa_I13514
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BBa_B0012
type: SequenceAnnotation
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display id: annotation1524886
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top level: BBa_I13514
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direction: direction/forward
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orientation: inline

BBa_I13105
type: ComponentDefinition
persistent identity: BBa I13105
display id: BBa_I13105
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Low output control (tet QPI)
creator: Barry Canton
created: 2004-07-20T11:00:00Z
modified: 2015-08-31T04:07:33Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_6_
m user id: 0
owner id: 135
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Lac promoter upstream
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Measurement
component: BBa_I13023
component: BBa_R0011
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sequence annotation: BBa_R0011
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display id: component2229278
version: 1
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access: public
definition: https://synbiohub.org/public/igem/BBa_I13023/1

BBa_R0011
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display id: component2229247
version: 1
top level: BBa_I13105
access: public
definition: https://synbiohub.org/public/igem/BBa_R0011/1

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version: 1
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direction: direction/reverse
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orientation: inline

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type: SequenceAnnotation
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display id: annotation2229247
version: 1
top level: BBa_I13105
location: https://synbiohub.org/public/igem/BBa_I13105/annotation2229247/range2229247/1
component: BBa_R0011

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type: Range
persistent identity: range2229247
display id: range2229247
version: 1
direction: direction/forward
top level: BBa_I13105
start: 1
end: 54
orientation: inline

BBa_K1682008
type: ComponentDefinition
persistent identity: BBa K1682008
display id: BBa_K1682008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PkdpF[-15, T>C] - GFP generator
creator: iGEM15_HKUST-Rice
created: 2015-08-10T11:00:00Z
modified: 2015-09-18T09:09:50Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2100_
m user id: 8758
owner id: 21235
owning group id: 9
status: status/Planning
bookmark: false
mutable description: kdpFp[-15, T>A] - E0240
mutable notes: kdpFp[-15, T>A] - E0240
mutable provenance: kdpFp[-15, T>A] - E0240
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Measurement
component: BBa_K1682003
component: BBa_E0240
sequence annotation: BBa_K1682003
sequence annotation: BBa_E0240
sequence: https://synbiohub.org/public/igem/BBa_K1682008_sequence/1

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type: Component
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display id: component2434943
version: 1
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access: public
definition: https://synbiohub.org/public/igem/BBa_K1682003/1

BBa_E0240
type: Component
persistent identity: component2434954
display id: component2434954
version: 1
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access: public
definition: https://synbiohub.org/public/igem/BBa_E0240/1

BBa_K1682003
type: SequenceAnnotation
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version: 1
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component: BBa_K1682003

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type: Range
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display id: range2434943
version: 1
direction: direction/reverse
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start: 1
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orientation: inline

BBa_E0240
type: SequenceAnnotation
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display id: annotation2434954
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type: Range
persistent identity: range2434954
display id: range2434954
version: 1
direction: direction/reverse
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BBa_I13113
type: ComponentDefinition
persistent identity: BBa I13113
display id: BBa_I13113
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: High Output control (c2 QPI)
creator: Barry Canton
created: 2004-07-20T11:00:00Z
modified: 2015-08-31T04:07:33Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_6_
m user id: 0
owner id: 135
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Terminator upstream, no promoter
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Measurement
component: BBa_B0012
component: BBa_R0053
component: BBa_B0010
component: BBa_B0034
component: BBa_C0053
component: BBa_E0030
sequence annotation: BBa_B0034
sequence annotation: BBa_B0012
sequence annotation: BBa_B0010
sequence annotation: BBa_C0053
sequence annotation: BBa_R0053
sequence annotation: BBa_E0030
sequence: https://synbiohub.org/public/igem/BBa_I13113_sequence/1

BBa_B0012
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persistent identity: component1021152
display id: component1021152
version: 1
top level: BBa_I13113
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_R0053
type: Component
persistent identity: component1021125
display id: component1021125
version: 1
top level: BBa_I13113
access: public
definition: https://synbiohub.org/public/igem/BBa_R0053/1

BBa_B0010
type: Component
persistent identity: component1021058
display id: component1021058
version: 1
top level: BBa_I13113
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0012
type: Component
persistent identity: component1021068
display id: component1021068
version: 1
top level: BBa_I13113
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0012
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display id: component1021106
version: 1
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access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0010
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display id: component1021096
version: 1
top level: BBa_I13113
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0010
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persistent identity: component1021142
display id: component1021142
version: 1
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access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0034
type: Component
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display id: component1021076
version: 1
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access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0034
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display id: component1021135
version: 1
top level: BBa_I13113
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_C0053
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display id: component1021088
version: 1
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access: public
definition: https://synbiohub.org/public/igem/BBa_C0053/1

BBa_E0030
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display id: component1021137
version: 1
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access: public
definition: https://synbiohub.org/public/igem/BBa_E0030/1

BBa_B0034
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version: 1
top level: BBa_I13113
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component: BBa_B0034

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display id: range1021135
version: 1
direction: direction/forward
top level: BBa_I13113
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end: 1086
orientation: inline

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version: 1
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display id: range1021068
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direction: direction/forward
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orientation: inline

BBa_B0010
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version: 1
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component: BBa_B0010

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direction: direction/forward
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orientation: inline

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direction: direction/forward
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component: BBa_B0010

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persistent identity: range1021096
display id: range1021096
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direction: direction/forward
top level: BBa_I13113
start: 876
end: 955
orientation: inline

BBa_B0012
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version: 1
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direction: direction/forward
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orientation: inline

BBa_B0012
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component: BBa_B0012

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persistent identity: range1021106
display id: range1021106
version: 1
direction: direction/forward
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start: 964
end: 1004
orientation: inline

BBa_R0053
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display id: annotation1021125
version: 1
top level: BBa_I13113
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component: BBa_R0053

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type: Range
persistent identity: range1021125
display id: range1021125
version: 1
direction: direction/forward
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start: 1013
end: 1066
orientation: inline

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display id: annotation1021076
version: 1
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component: BBa_B0034

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type: Range
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display id: range1021076
version: 1
direction: direction/forward
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start: 138
end: 149
orientation: inline

BBa_E0030
type: SequenceAnnotation
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display id: annotation1021137
version: 1
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component: BBa_E0030

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persistent identity: range1021137
display id: range1021137
version: 1
direction: direction/forward
top level: BBa_I13113
start: 1093
end: 1815
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1021142
display id: annotation1021142
version: 1
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location: https://synbiohub.org/public/igem/BBa_I13113/annotation1021142/range1021142/1
component: BBa_B0010

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type: Range
persistent identity: range1021142
display id: range1021142
version: 1
direction: direction/forward
top level: BBa_I13113
start: 1824
end: 1903
orientation: inline

BBa_J07028
type: ComponentDefinition
persistent identity: BBa J07028
display id: BBa_J07028
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: ToxR'::MalE(with promoter and RBS and terminator)
creator: jenmitch
created: 2005-07-13T11:00:00Z
modified: 2015-08-31T04:08:19Z
discontinued: false
dominant: false
experience: experience/None
group u list: _21_
m user id: 0
owner id: 164
owning group id: 21
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This part is a negative control for ToxR' dimerization. MalE only exists as a monomer, and when fused to ToxR', will prevent it from dimerizing.
mutable provenance: composite part
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Measurement
component: BBa_B0015
component: BBa_B0030
component: BBa_R0040
component: BBa_J07009
component: BBa_J07006
sequence annotation: BBa_B0015
sequence annotation: BBa_J07009
sequence annotation: BBa_R0040
sequence annotation: BBa_B0030
sequence annotation: BBa_J07006
sequence: https://synbiohub.org/public/igem/BBa_J07028_sequence/1

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persistent identity: component2223773
display id: component2223773
version: 1
top level: BBa_J07028
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_B0030
type: Component
persistent identity: component2223757
display id: component2223757
version: 1
top level: BBa_J07028
access: public
definition: https://synbiohub.org/public/igem/BBa_B0030/1

BBa_R0040
type: Component
persistent identity: component2223751
display id: component2223751
version: 1
top level: BBa_J07028
access: public
definition: https://synbiohub.org/public/igem/BBa_R0040/1

BBa_J07009
type: Component
persistent identity: component2223764
display id: component2223764
version: 1
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access: public
definition: https://synbiohub.org/public/igem/BBa_J07009/1

BBa_J07006
type: Component
persistent identity: component2223766
display id: component2223766
version: 1
top level: BBa_J07028
access: public
definition: https://synbiohub.org/public/igem/BBa_J07006/1

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2223773
display id: annotation2223773
version: 1
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location: https://synbiohub.org/public/igem/BBa_J07028/annotation2223773/range2223773/1
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type: Range
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type: SequenceAnnotation
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display id: annotation2223764
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component: BBa_J07009

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display id: range2223764
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direction: direction/reverse
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BBa_R0040
type: SequenceAnnotation
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BBa_J07006
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BBa_I13011
type: ComponentDefinition
persistent identity: BBa I13011
display id: BBa_I13011
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS Test R0040.B0030.E0130
creator: jasonk
created: 2004-06-30T11:00:00Z
modified: 2015-08-31T04:07:32Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_6_
m user id: 0
owner id: 135
owning group id: 7
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Parts I13007-I130014 are RBS test constructs for RBS characterization. Each consists of Lac or Tet promoter, one of the four RBS's B0030-B0033, a YFP coding protein, and B0015 terminator.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Measurement
component: BBa_B0030
component: BBa_B0010
component: BBa_R0040
component: BBa_E0030
component: BBa_B0012
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_B0030
sequence annotation: BBa_R0040
sequence annotation: BBa_E0030
sequence: https://synbiohub.org/public/igem/BBa_I13011_sequence/1

BBa_B0030
type: Component
persistent identity: component946779
display id: component946779
version: 1
top level: BBa_I13011
access: public
definition: https://synbiohub.org/public/igem/BBa_B0030/1

BBa_B0010
type: Component
persistent identity: component946787
display id: component946787
version: 1
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access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_R0040
type: Component
persistent identity: component946768
display id: component946768
version: 1
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access: public
definition: https://synbiohub.org/public/igem/BBa_R0040/1

BBa_E0030
type: Component
persistent identity: component946782
display id: component946782
version: 1
top level: BBa_I13011
access: public
definition: https://synbiohub.org/public/igem/BBa_E0030/1

BBa_B0012
type: Component
persistent identity: component946797
display id: component946797
version: 1
top level: BBa_I13011
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation946787
display id: annotation946787
version: 1
top level: BBa_I13011
location: https://synbiohub.org/public/igem/BBa_I13011/annotation946787/range946787/1
component: BBa_B0010

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orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation946797
display id: annotation946797
version: 1
top level: BBa_I13011
location: https://synbiohub.org/public/igem/BBa_I13011/annotation946797/range946797/1
component: BBa_B0012

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BBa_B0030
type: SequenceAnnotation
persistent identity: annotation946779
display id: annotation946779
version: 1
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component: BBa_B0030

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BBa_R0040
type: SequenceAnnotation
persistent identity: annotation946768
display id: annotation946768
version: 1
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location: https://synbiohub.org/public/igem/BBa_I13011/annotation946768/range946768/1
component: BBa_R0040

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type: Range
persistent identity: range946768
display id: range946768
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direction: direction/forward
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start: 1
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BBa_E0030
type: SequenceAnnotation
persistent identity: annotation946782
display id: annotation946782
version: 1
top level: BBa_I13011
location: https://synbiohub.org/public/igem/BBa_I13011/annotation946782/range946782/1
component: BBa_E0030

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display id: range946782
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start: 84
end: 806
orientation: inline

BBa_K1682007
type: ComponentDefinition
persistent identity: BBa K1682007
display id: BBa_K1682007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PkdpF[-15, T>A] - GFP generator
creator: iGEM15_HKUST-Rice
created: 2015-08-10T11:00:00Z
modified: 2015-09-18T09:10:21Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2100_
m user id: 8758
owner id: 21235
owning group id: 9
status: status/Planning
bookmark: false
mutable description: kdpFp[-15, T>A]
mutable notes: kdpFp[-15, T>A]
mutable provenance: kdpFp[-15, T>A]
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Measurement
component: BBa_K1682002
component: BBa_E0240
sequence annotation: BBa_K1682002
sequence annotation: BBa_E0240
sequence: https://synbiohub.org/public/igem/BBa_K1682007_sequence/1

BBa_K1682002
type: Component
persistent identity: component2434917
display id: component2434917
version: 1
top level: BBa_K1682007
access: public
definition: https://synbiohub.org/public/igem/BBa_K1682002/1

BBa_E0240
type: Component
persistent identity: component2434928
display id: component2434928
version: 1
top level: BBa_K1682007
access: public
definition: https://synbiohub.org/public/igem/BBa_E0240/1

BBa_K1682002
type: SequenceAnnotation
persistent identity: annotation2434917
display id: annotation2434917
version: 1
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location: https://synbiohub.org/public/igem/BBa_K1682007/annotation2434917/range2434917/1
component: BBa_K1682002

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type: SequenceAnnotation
persistent identity: annotation2434928
display id: annotation2434928
version: 1
top level: BBa_K1682007
location: https://synbiohub.org/public/igem/BBa_K1682007/annotation2434928/range2434928/1
component: BBa_E0240

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type: Range
persistent identity: range2434928
display id: range2434928
version: 1
direction: direction/reverse
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start: 87
end: 962
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BBa_I13515
type: ComponentDefinition
persistent identity: BBa I13515
display id: BBa_I13515
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Screening Plasmid 4
creator: jkm
created: 2005-05-30T11:00:00Z
modified: 2015-08-31T04:07:34Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _11_
m user id: 0
owner id: 253
owning group id: 6
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: The fourth screening plasmid. Same as the second, except without an insertion site. Sole purpose is to check whether the insertion site alone affects the performance. Ends should still be capped with I13450.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Measurement
component: BBa_E0040
component: BBa_B0034
component: BBa_B0010
component: BBa_I0500
component: BBa_B0012
component: BBa_E1010
sequence annotation: BBa_E1010
sequence annotation: BBa_B0010
sequence annotation: BBa_E0040
sequence annotation: BBa_B0012
sequence annotation: BBa_B0034
sequence annotation: BBa_I0500
sequence: https://synbiohub.org/public/igem/BBa_I13515_sequence/1

BBa_E0040
type: Component
persistent identity: component1505456
display id: component1505456
version: 1
top level: BBa_I13515
access: public
definition: https://synbiohub.org/public/igem/BBa_E0040/1

BBa_B0034
type: Component
persistent identity: component1505454
display id: component1505454
version: 1
top level: BBa_I13515
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0010
type: Component
persistent identity: component1505461
display id: component1505461
version: 1
top level: BBa_I13515
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_I0500
type: Component
persistent identity: component1505436
display id: component1505436
version: 1
top level: BBa_I13515
access: public
definition: https://synbiohub.org/public/igem/BBa_I0500/1

BBa_B0012
type: Component
persistent identity: component1505471
display id: component1505471
version: 1
top level: BBa_I13515
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_E1010
type: Component
persistent identity: component1505448
display id: component1505448
version: 1
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access: public
definition: https://synbiohub.org/public/igem/BBa_E1010/1

BBa_B0034
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persistent identity: component1505441
display id: component1505441
version: 1
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access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

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type: SequenceAnnotation
persistent identity: annotation1505448
display id: annotation1505448
version: 1
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location: https://synbiohub.org/public/igem/BBa_I13515/annotation1505448/range1505448/1
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version: 1
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component: BBa_B0010

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BBa_E0040
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display id: annotation1505456
version: 1
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direction: direction/forward
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start: 1969
end: 2688
orientation: inline

BBa_B0012
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display id: annotation1505471
version: 1
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component: BBa_B0012

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display id: range1505471
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direction: direction/forward
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start: 2785
end: 2825
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BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1505441
display id: annotation1505441
version: 1
top level: BBa_I13515
location: https://synbiohub.org/public/igem/BBa_I13515/annotation1505441/range1505441/1
component: BBa_B0034

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persistent identity: range1505441
display id: range1505441
version: 1
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start: 1219
end: 1230
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type: SequenceAnnotation
persistent identity: annotation1505454
display id: annotation1505454
version: 1
top level: BBa_I13515
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component: BBa_B0034

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type: Range
persistent identity: range1505454
display id: range1505454
version: 1
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start: 1951
end: 1962
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BBa_I0500
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persistent identity: annotation1505436
display id: annotation1505436
version: 1
top level: BBa_I13515
location: https://synbiohub.org/public/igem/BBa_I13515/annotation1505436/range1505436/1
component: BBa_I0500

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persistent identity: range1505436
display id: range1505436
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direction: direction/forward
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start: 1
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BBa_J07005
type: ComponentDefinition
persistent identity: BBa J07005
display id: BBa_J07005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PhoA (E.coli JM83 alkaline phosphatase gene)
creator: igem_mit_2005
created: 2005-07-04T11:00:00Z
modified: 2015-08-31T04:08:19Z
discontinued: false
dominant: false
experience: experience/None
group u list: _21_
m user id: 0
owner id: 363
owning group id: 21
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: phoA can substituted for the periplasmic region of ToxR transmembrane dimerizing protein. igemmit2005 will be using this as a positive control in tests related to ToxR pathway.
mutable provenance: http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Measurement
sequence annotation: PhoA
sequence: https://synbiohub.org/public/igem/BBa_J07005_sequence/1

PhoA
type: SequenceAnnotation
persistent identity: annotation1571543
display id: annotation1571543
version: 1
top level: BBa_J07005
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J07005/annotation1571543/range1571543/1

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type: Range
persistent identity: range1571543
display id: range1571543
version: 1
direction: direction/forward
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start: 1
end: 1416
orientation: inline

BBa_I13621
type: ComponentDefinition
persistent identity: BBa I13621
display id: BBa_I13621
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Cascaded Lac (weak) and Tet (medium) QPIs
creator: mit
created: 2004-07-19T11:00:00Z
modified: 2015-08-31T04:07:36Z
discontinued: false
dominant: false
experience: experience/None
group u list: _6_
m user id: 0
owner id: 101
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Measurement
component: BBa_Q03121
component: BBa_Q01400
sequence annotation: BBa_Q03121
sequence annotation: BBa_Q01400
sequence: https://synbiohub.org/public/igem/BBa_I13621_sequence/1

BBa_Q03121
type: Component
persistent identity: component2228902
display id: component2228902
version: 1
top level: BBa_I13621
access: public
definition: https://synbiohub.org/public/igem/BBa_Q03121/1

BBa_Q01400
type: Component
persistent identity: component2228920
display id: component2228920
version: 1
top level: BBa_I13621
access: public
definition: https://synbiohub.org/public/igem/BBa_Q01400/1

BBa_Q03121
type: SequenceAnnotation
persistent identity: annotation2228902
display id: annotation2228902
version: 1
top level: BBa_I13621
location: https://synbiohub.org/public/igem/BBa_I13621/annotation2228902/range2228902/1
component: BBa_Q03121

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type: Range
persistent identity: range2228902
display id: range2228902
version: 1
direction: direction/reverse
top level: BBa_I13621
start: 1
end: 1370
orientation: inline

BBa_Q01400
type: SequenceAnnotation
persistent identity: annotation2228920
display id: annotation2228920
version: 1
top level: BBa_I13621
location: https://synbiohub.org/public/igem/BBa_I13621/annotation2228920/range2228920/1
component: BBa_Q01400

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type: Range
persistent identity: range2228920
display id: range2228920
version: 1
direction: direction/reverse
top level: BBa_I13621
start: 1379
end: 2282
orientation: inline

BBa_I13637
type: ComponentDefinition
persistent identity: BBa I13637
display id: BBa_I13637
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Cascaded Tet (elowitz) and Lac (medium) QPIs with double terminator as input
creator: mit
created: 2004-07-19T11:00:00Z
modified: 2015-08-31T04:07:36Z
discontinued: false
dominant: false
experience: experience/None
group u list: _6_
m user id: 0
owner id: 101
owning group id: 7
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Measurement
component: BBa_Q04400
component: BBa_Q01121
component: BBa_B0015
sequence annotation: BBa_Q04400
sequence annotation: BBa_B0015
sequence annotation: BBa_Q01121
sequence: https://synbiohub.org/public/igem/BBa_I13637_sequence/1

BBa_Q04400
type: Component
persistent identity: component2232361
display id: component2232361
version: 1
top level: BBa_I13637
access: public
definition: https://synbiohub.org/public/igem/BBa_Q04400/1

BBa_Q01121
type: Component
persistent identity: component2232380
display id: component2232380
version: 1
top level: BBa_I13637
access: public
definition: https://synbiohub.org/public/igem/BBa_Q01121/1

BBa_B0015
type: Component
persistent identity: component2232343
display id: component2232343
version: 1
top level: BBa_I13637
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_Q04400
type: SequenceAnnotation
persistent identity: annotation2232361
display id: annotation2232361
version: 1
top level: BBa_I13637
location: https://synbiohub.org/public/igem/BBa_I13637/annotation2232361/range2232361/1
component: BBa_Q04400

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type: Range
persistent identity: range2232361
display id: range2232361
version: 1
direction: direction/reverse
top level: BBa_I13637
start: 138
end: 1039
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2232343
display id: annotation2232343
version: 1
top level: BBa_I13637
location: https://synbiohub.org/public/igem/BBa_I13637/annotation2232343/range2232343/1
component: BBa_B0015

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type: Range
persistent identity: range2232343
display id: range2232343
version: 1
direction: direction/reverse
top level: BBa_I13637
start: 1
end: 129
orientation: inline

BBa_Q01121
type: SequenceAnnotation
persistent identity: annotation2232380
display id: annotation2232380
version: 1
top level: BBa_I13637
location: https://synbiohub.org/public/igem/BBa_I13637/annotation2232380/range2232380/1
component: BBa_Q01121

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type: Range
persistent identity: range2232380
display id: range2232380
version: 1
direction: direction/reverse
top level: BBa_I13637
start: 1048
end: 2420
orientation: inline

BBa_I13111
type: ComponentDefinition
persistent identity: BBa I13111
display id: BBa_I13111
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Low output control (lac QPI, B0031 RBS)
creator: Barry Canton
created: 2004-07-20T11:00:00Z
modified: 2015-08-31T04:07:33Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_6_
m user id: 0
owner id: 135
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: tet promoter upstream
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Measurement
component: BBa_I13024
component: BBa_R0040
sequence annotation: BBa_I13024
sequence annotation: BBa_R0040
sequence: https://synbiohub.org/public/igem/BBa_I13111_sequence/1

BBa_I13024
type: Component
persistent identity: component2229415
display id: component2229415
version: 1
top level: BBa_I13111
access: public
definition: https://synbiohub.org/public/igem/BBa_I13024/1

BBa_R0040
type: Component
persistent identity: component2229381
display id: component2229381
version: 1
top level: BBa_I13111
access: public
definition: https://synbiohub.org/public/igem/BBa_R0040/1

BBa_I13024
type: SequenceAnnotation
persistent identity: annotation2229415
display id: annotation2229415
version: 1
top level: BBa_I13111
location: https://synbiohub.org/public/igem/BBa_I13111/annotation2229415/range2229415/1
component: BBa_I13024

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type: Range
persistent identity: range2229415
display id: range2229415
version: 1
direction: direction/reverse
top level: BBa_I13111
start: 63
end: 2321
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation2229381
display id: annotation2229381
version: 1
top level: BBa_I13111
location: https://synbiohub.org/public/igem/BBa_I13111/annotation2229381/range2229381/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_I13111/annotation2229381/range2229381/1
type: Range
persistent identity: range2229381
display id: range2229381
version: 1
direction: direction/forward
top level: BBa_I13111
start: 1
end: 54
orientation: inline

BBa_I0421
type: ComponentDefinition
persistent identity: BBa I0421
display id: BBa_I0421
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: R0011.E0030.B0015
creator: jcbraff
created: 2004-06-13T11:00:00Z
modified: 2015-08-31T04:07:29Z
discontinued: false
dominant: true
experience: experience/None
group u list: _11_1_
m user id: 0
owner id: 61
owning group id: 7
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: R0011.E0030.B0015 RBS Test control
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Measurement
component: BBa_R0011
component: BBa_E0030
component: BBa_B0015
sequence annotation: BBa_E0030
sequence annotation: BBa_R0011
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_I0421_sequence/1

BBa_R0011
type: Component
persistent identity: component2220189
display id: component2220189
version: 1
top level: BBa_I0421
access: public
definition: https://synbiohub.org/public/igem/BBa_R0011/1

BBa_E0030
type: Component
persistent identity: component2220194
display id: component2220194
version: 1
top level: BBa_I0421
access: public
definition: https://synbiohub.org/public/igem/BBa_E0030/1

BBa_B0015
type: Component
persistent identity: component2220201
display id: component2220201
version: 1
top level: BBa_I0421
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_E0030
type: SequenceAnnotation
persistent identity: annotation2220194
display id: annotation2220194
version: 1
top level: BBa_I0421
location: https://synbiohub.org/public/igem/BBa_I0421/annotation2220194/range2220194/1
component: BBa_E0030

https://synbiohub.org/public/igem/BBa_I0421/annotation2220194/range2220194/1
type: Range
persistent identity: range2220194
display id: range2220194
version: 1
direction: direction/reverse
top level: BBa_I0421
start: 62
end: 784
orientation: inline

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation2220189
display id: annotation2220189
version: 1
top level: BBa_I0421
location: https://synbiohub.org/public/igem/BBa_I0421/annotation2220189/range2220189/1
component: BBa_R0011

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type: Range
persistent identity: range2220189
display id: range2220189
version: 1
direction: direction/forward
top level: BBa_I0421
start: 1
end: 54
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2220201
display id: annotation2220201
version: 1
top level: BBa_I0421
location: https://synbiohub.org/public/igem/BBa_I0421/annotation2220201/range2220201/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_I0421/annotation2220201/range2220201/1
type: Range
persistent identity: range2220201
display id: range2220201
version: 1
direction: direction/reverse
top level: BBa_I0421
start: 793
end: 921
orientation: inline

BBa_K389012
type: ComponentDefinition
persistent identity: BBa K389012
display id: BBa_K389012
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: VirA reporter system luc
creator: Jonas Aretz
created: 2010-09-10T11:00:00Z
modified: 2015-05-08T01:12:19Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _517_
m user id: 0
owner id: 6670
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: With this system it is possible to measure the activity of a ''virA'' receptor and the ''vir'' promoter, respectively. The reporter is a firefly luciferase from Promega's pGL4.10luc2 plasmid.
mutable notes: medium strong constitutive promotor, terminator to keep basal expression of luciferase low
mutable provenance: partsregistry, synthetic gene by Mr. Gene, pGL4.10luc2 by Promega, TI-plasmid from ''Agrobacterium tumefaciens'' C58
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K389003
component: BBa_B0010
component: BBa_J23110
component: BBa_K389002
component: BBa_K389004
sequence annotation: BBa_K389004
sequence annotation: BBa_J23110
sequence annotation: BBa_B0010
sequence annotation: BBa_K389003
sequence annotation: BBa_K389002
sequence: https://synbiohub.org/public/igem/BBa_K389012_sequence/1

BBa_K389003
type: Component
persistent identity: component2079841
display id: component2079841
version: 1
top level: BBa_K389012
access: public
definition: https://synbiohub.org/public/igem/BBa_K389003/1

BBa_B0010
type: Component
persistent identity: component2079835
display id: component2079835
version: 1
top level: BBa_K389012
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0010
type: Component
persistent identity: component2079833
display id: component2079833
version: 1
top level: BBa_K389012
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_J23110
type: Component
persistent identity: component2079828
display id: component2079828
version: 1
top level: BBa_K389012
access: public
definition: https://synbiohub.org/public/igem/BBa_J23110/1

BBa_K389002
type: Component
persistent identity: component2079832
display id: component2079832
version: 1
top level: BBa_K389012
access: public
definition: https://synbiohub.org/public/igem/BBa_K389002/1

BBa_K389004
type: Component
persistent identity: component2079844
display id: component2079844
version: 1
top level: BBa_K389012
access: public
definition: https://synbiohub.org/public/igem/BBa_K389004/1

BBa_K389004
type: SequenceAnnotation
persistent identity: annotation2079844
display id: annotation2079844
version: 1
top level: BBa_K389012
location: https://synbiohub.org/public/igem/BBa_K389012/annotation2079844/range2079844/1
component: BBa_K389004

https://synbiohub.org/public/igem/BBa_K389012/annotation2079844/range2079844/1
type: Range
persistent identity: range2079844
display id: range2079844
version: 1
direction: direction/reverse
top level: BBa_K389012
start: 1132
end: 2787
orientation: inline

BBa_J23110
type: SequenceAnnotation
persistent identity: annotation2079828
display id: annotation2079828
version: 1
top level: BBa_K389012
location: https://synbiohub.org/public/igem/BBa_K389012/annotation2079828/range2079828/1
component: BBa_J23110

https://synbiohub.org/public/igem/BBa_K389012/annotation2079828/range2079828/1
type: Range
persistent identity: range2079828
display id: range2079828
version: 1
direction: direction/reverse
top level: BBa_K389012
start: 1
end: 35
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2079835
display id: annotation2079835
version: 1
top level: BBa_K389012
location: https://synbiohub.org/public/igem/BBa_K389012/annotation2079835/range2079835/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K389012/annotation2079835/range2079835/1
type: Range
persistent identity: range2079835
display id: range2079835
version: 1
direction: direction/forward
top level: BBa_K389012
start: 880
end: 959
orientation: inline

BBa_K389003
type: SequenceAnnotation
persistent identity: annotation2079841
display id: annotation2079841
version: 1
top level: BBa_K389012
location: https://synbiohub.org/public/igem/BBa_K389012/annotation2079841/range2079841/1
component: BBa_K389003

https://synbiohub.org/public/igem/BBa_K389012/annotation2079841/range2079841/1
type: Range
persistent identity: range2079841
display id: range2079841
version: 1
direction: direction/reverse
top level: BBa_K389012
start: 968
end: 1125
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2079833
display id: annotation2079833
version: 1
top level: BBa_K389012
location: https://synbiohub.org/public/igem/BBa_K389012/annotation2079833/range2079833/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K389012/annotation2079833/range2079833/1
type: Range
persistent identity: range2079833
display id: range2079833
version: 1
direction: direction/forward
top level: BBa_K389012
start: 792
end: 871
orientation: inline

BBa_K389002
type: SequenceAnnotation
persistent identity: annotation2079832
display id: annotation2079832
version: 1
top level: BBa_K389012
location: https://synbiohub.org/public/igem/BBa_K389012/annotation2079832/range2079832/1
component: BBa_K389002

https://synbiohub.org/public/igem/BBa_K389012/annotation2079832/range2079832/1
type: Range
persistent identity: range2079832
display id: range2079832
version: 1
direction: direction/reverse
top level: BBa_K389012
start: 44
end: 783
orientation: inline

BBa_I13101
type: ComponentDefinition
persistent identity: BBa I13101
display id: BBa_I13101
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: cII Self-coupled Inverter
creator: Barry Canton
created: 2004-07-20T11:00:00Z
modified: 2015-08-31T04:07:33Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_6_
m user id: 0
owner id: 135
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Measurement
component: BBa_I13020
component: BBa_R0053
sequence annotation: BBa_I13020
sequence annotation: BBa_R0053
sequence: https://synbiohub.org/public/igem/BBa_I13101_sequence/1

BBa_I13020
type: Component
persistent identity: component2229144
display id: component2229144
version: 1
top level: BBa_I13101
access: public
definition: https://synbiohub.org/public/igem/BBa_I13020/1

BBa_R0053
type: Component
persistent identity: component2229109
display id: component2229109
version: 1
top level: BBa_I13101
access: public
definition: https://synbiohub.org/public/igem/BBa_R0053/1

BBa_I13020
type: SequenceAnnotation
persistent identity: annotation2229144
display id: annotation2229144
version: 1
top level: BBa_I13101
location: https://synbiohub.org/public/igem/BBa_I13101/annotation2229144/range2229144/1
component: BBa_I13020

https://synbiohub.org/public/igem/BBa_I13101/annotation2229144/range2229144/1
type: Range
persistent identity: range2229144
display id: range2229144
version: 1
direction: direction/reverse
top level: BBa_I13101
start: 63
end: 1877
orientation: inline

BBa_R0053
type: SequenceAnnotation
persistent identity: annotation2229109
display id: annotation2229109
version: 1
top level: BBa_I13101
location: https://synbiohub.org/public/igem/BBa_I13101/annotation2229109/range2229109/1
component: BBa_R0053

https://synbiohub.org/public/igem/BBa_I13101/annotation2229109/range2229109/1
type: Range
persistent identity: range2229109
display id: range2229109
version: 1
direction: direction/forward
top level: BBa_I13101
start: 1
end: 54
orientation: inline

BBa_I12240
type: ComponentDefinition
persistent identity: BBa I12240
display id: BBa_I12240
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Test of LacI protein generator
creator: rwald
created: 2004-08-31T11:00:00Z
modified: 2015-08-31T04:07:32Z
discontinued: false
dominant: false
experience: experience/None
group u list: _3_
m user id: 0
owner id: 120
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This part tests the effecacy of the I12224 protein generator.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Measurement
component: BBa_R0011
component: BBa_R0053
component: BBa_E0422
component: BBa_I12224
sequence annotation: BBa_I12224
sequence annotation: BBa_E0422
sequence annotation: BBa_R0011
sequence annotation: BBa_R0053
sequence: https://synbiohub.org/public/igem/BBa_I12240_sequence/1

BBa_R0011
type: Component
persistent identity: component2229840
display id: component2229840
version: 1
top level: BBa_I12240
access: public
definition: https://synbiohub.org/public/igem/BBa_R0011/1

BBa_R0053
type: Component
persistent identity: component2229822
display id: component2229822
version: 1
top level: BBa_I12240
access: public
definition: https://synbiohub.org/public/igem/BBa_R0053/1

BBa_E0422
type: Component
persistent identity: component2229858
display id: component2229858
version: 1
top level: BBa_I12240
access: public
definition: https://synbiohub.org/public/igem/BBa_E0422/1

BBa_I12224
type: Component
persistent identity: component2229839
display id: component2229839
version: 1
top level: BBa_I12240
access: public
definition: https://synbiohub.org/public/igem/BBa_I12224/1

BBa_I12224
type: SequenceAnnotation
persistent identity: annotation2229839
display id: annotation2229839
version: 1
top level: BBa_I12240
location: https://synbiohub.org/public/igem/BBa_I12240/annotation2229839/range2229839/1
component: BBa_I12224

https://synbiohub.org/public/igem/BBa_I12240/annotation2229839/range2229839/1
type: Range
persistent identity: range2229839
display id: range2229839
version: 1
direction: direction/reverse
top level: BBa_I12240
start: 63
end: 1329
orientation: inline

BBa_E0422
type: SequenceAnnotation
persistent identity: annotation2229858
display id: annotation2229858
version: 1
top level: BBa_I12240
location: https://synbiohub.org/public/igem/BBa_I12240/annotation2229858/range2229858/1
component: BBa_E0422

https://synbiohub.org/public/igem/BBa_I12240/annotation2229858/range2229858/1
type: Range
persistent identity: range2229858
display id: range2229858
version: 1
direction: direction/reverse
top level: BBa_I12240
start: 1401
end: 2317
orientation: inline

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation2229840
display id: annotation2229840
version: 1
top level: BBa_I12240
location: https://synbiohub.org/public/igem/BBa_I12240/annotation2229840/range2229840/1
component: BBa_R0011

https://synbiohub.org/public/igem/BBa_I12240/annotation2229840/range2229840/1
type: Range
persistent identity: range2229840
display id: range2229840
version: 1
direction: direction/forward
top level: BBa_I12240
start: 1338
end: 1391
orientation: inline

BBa_R0053
type: SequenceAnnotation
persistent identity: annotation2229822
display id: annotation2229822
version: 1
top level: BBa_I12240
location: https://synbiohub.org/public/igem/BBa_I12240/annotation2229822/range2229822/1
component: BBa_R0053

https://synbiohub.org/public/igem/BBa_I12240/annotation2229822/range2229822/1
type: Range
persistent identity: range2229822
display id: range2229822
version: 1
direction: direction/forward
top level: BBa_I12240
start: 1
end: 54
orientation: inline

BBa_I12241
type: ComponentDefinition
persistent identity: BBa I12241
display id: BBa_I12241
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Test of TetR protein generator
creator: rwald
created: 2004-08-31T11:00:00Z
modified: 2015-08-31T04:07:32Z
discontinued: false
dominant: false
experience: experience/None
group u list: _3_
m user id: 0
owner id: 120
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This component tests the I12222 TetR protein generator.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Measurement
component: BBa_R0040
component: BBa_B0012
component: BBa_B0010
component: BBa_I12202
component: BBa_B0032
component: BBa_E0022
component: BBa_R0053
component: BBa_B0034
sequence annotation: BBa_R0053
sequence annotation: BBa_B0010
sequence annotation: BBa_E0022
sequence annotation: BBa_B0032
sequence annotation: BBa_B0012
sequence annotation: BBa_I12202
sequence annotation: BBa_B0034
sequence annotation: BBa_R0040
sequence: https://synbiohub.org/public/igem/BBa_I12241_sequence/1

BBa_R0040
type: Component
persistent identity: component1062838
display id: component1062838
version: 1
top level: BBa_I12241
access: public
definition: https://synbiohub.org/public/igem/BBa_R0040/1

BBa_B0012
type: Component
persistent identity: component1062823
display id: component1062823
version: 1
top level: BBa_I12241
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0012
type: Component
persistent identity: component1062876
display id: component1062876
version: 1
top level: BBa_I12241
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0010
type: Component
persistent identity: component1062866
display id: component1062866
version: 1
top level: BBa_I12241
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_I12202
type: Component
persistent identity: component1062807
display id: component1062807
version: 1
top level: BBa_I12241
access: public
definition: https://synbiohub.org/public/igem/BBa_I12202/1

BBa_B0032
type: Component
persistent identity: component1062799
display id: component1062799
version: 1
top level: BBa_I12241
access: public
definition: https://synbiohub.org/public/igem/BBa_B0032/1

BBa_B0010
type: Component
persistent identity: component1062813
display id: component1062813
version: 1
top level: BBa_I12241
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_E0022
type: Component
persistent identity: component1062858
display id: component1062858
version: 1
top level: BBa_I12241
access: public
definition: https://synbiohub.org/public/igem/BBa_E0022/1

BBa_R0053
type: Component
persistent identity: component1062786
display id: component1062786
version: 1
top level: BBa_I12241
access: public
definition: https://synbiohub.org/public/igem/BBa_R0053/1

BBa_B0034
type: Component
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description: RBS Test R0011.B0031.E0030.B0015
creator: jcbraff
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modified: 2015-08-31T04:07:28Z
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was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Reporter Device Powered by the Virtual Machine (VM1.0)
creator: Barry Canton
created: 2005-06-05T11:00:00Z
modified: 2015-08-31T04:07:27Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_
m user id: 0
owner id: 135
owning group id: 6
sample status: Not in stock
status: status/Unavailable
bookmark: false
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display id: range979326
version: 1
direction: direction/forward
top level: BBa_I6013
start: 3380
end: 4129
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation979365
display id: annotation979365
version: 1
top level: BBa_I6013
location: https://synbiohub.org/public/igem/BBa_I6013/annotation979365/range979365/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I6013/annotation979365/range979365/1
type: Range
persistent identity: range979365
display id: range979365
version: 1
direction: direction/forward
top level: BBa_I6013
start: 4357
end: 4368
orientation: inline

BBa_I0500
type: SequenceAnnotation
persistent identity: annotation979271
display id: annotation979271
version: 1
top level: BBa_I6013
location: https://synbiohub.org/public/igem/BBa_I6013/annotation979271/range979271/1
component: BBa_I0500

https://synbiohub.org/public/igem/BBa_I6013/annotation979271/range979271/1
type: Range
persistent identity: range979271
display id: range979271
version: 1
direction: direction/forward
top level: BBa_I6013
start: 1
end: 1210
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation979296
display id: annotation979296
version: 1
top level: BBa_I6013
location: https://synbiohub.org/public/igem/BBa_I6013/annotation979296/range979296/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I6013/annotation979296/range979296/1
type: Range
persistent identity: range979296
display id: range979296
version: 1
direction: direction/forward
top level: BBa_I6013
start: 2007
end: 2086
orientation: inline

BBa_I0500
type: SequenceAnnotation
persistent identity: annotation979311
display id: annotation979311
version: 1
top level: BBa_I6013
location: https://synbiohub.org/public/igem/BBa_I6013/annotation979311/range979311/1
component: BBa_I0500

https://synbiohub.org/public/igem/BBa_I6013/annotation979311/range979311/1
type: Range
persistent identity: range979311
display id: range979311
version: 1
direction: direction/forward
top level: BBa_I6013
start: 2144
end: 3353
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation979342
display id: annotation979342
version: 1
top level: BBa_I6013
location: https://synbiohub.org/public/igem/BBa_I6013/annotation979342/range979342/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I6013/annotation979342/range979342/1
type: Range
persistent identity: range979342
display id: range979342
version: 1
direction: direction/forward
top level: BBa_I6013
start: 4251
end: 4291
orientation: inline

BBa_E0022
type: SequenceAnnotation
persistent identity: annotation979288
display id: annotation979288
version: 1
top level: BBa_I6013
location: https://synbiohub.org/public/igem/BBa_I6013/annotation979288/range979288/1
component: BBa_E0022

https://synbiohub.org/public/igem/BBa_I6013/annotation979288/range979288/1
type: Range
persistent identity: range979288
display id: range979288
version: 1
direction: direction/forward
top level: BBa_I6013
start: 1237
end: 1998
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation979276
display id: annotation979276
version: 1
top level: BBa_I6013
location: https://synbiohub.org/public/igem/BBa_I6013/annotation979276/range979276/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I6013/annotation979276/range979276/1
type: Range
persistent identity: range979276
display id: range979276
version: 1
direction: direction/forward
top level: BBa_I6013
start: 1219
end: 1230
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation979332
display id: annotation979332
version: 1
top level: BBa_I6013
location: https://synbiohub.org/public/igem/BBa_I6013/annotation979332/range979332/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I6013/annotation979332/range979332/1
type: Range
persistent identity: range979332
display id: range979332
version: 1
direction: direction/forward
top level: BBa_I6013
start: 4163
end: 4242
orientation: inline

BBa_K1682015
type: ComponentDefinition
persistent identity: BBa K1682015
display id: BBa_K1682015
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PphoBR-I13504
creator: iGEM15_HKUST-Rice
created: 2015-09-09T11:00:00Z
modified: 2015-09-18T10:30:52Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2100_
m user id: 16893
owner id: 26069
owning group id: 9
status: status/Planning
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Measurement
sequence annotation: phospho-phoB Bindind site
sequence: https://synbiohub.org/public/igem/BBa_K1682015_sequence/1

phospho-phoB Bindind site
type: SequenceAnnotation
persistent identity: annotation2475990
display id: annotation2475990
version: 1
top level: BBa_K1682015
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1682015/annotation2475990/range2475990/1

https://synbiohub.org/public/igem/BBa_K1682015/annotation2475990/range2475990/1
type: Range
persistent identity: range2475990
display id: range2475990
version: 1
direction: direction/forward
top level: BBa_K1682015
start: 21
end: 39
orientation: inline

BBa_I13012
type: ComponentDefinition
persistent identity: BBa I13012
display id: BBa_I13012
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS Test R0040.B0031.E0130
creator: jasonk
created: 2004-06-30T11:00:00Z
modified: 2015-08-31T04:07:32Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_6_
m user id: 0
owner id: 135
owning group id: 7
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Parts I13007-I130014 are RBS test constructs for RBS characterization. Each consists of Lac or Tet promoter, one of the four RBS's B0030-B0033, a YFP coding protein, and B0015 terminator.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Measurement
component: BBa_B0010
component: BBa_B0031
component: BBa_B0012
component: BBa_E0030
component: BBa_R0040
sequence annotation: BBa_R0040
sequence annotation: BBa_E0030
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_B0031
sequence: https://synbiohub.org/public/igem/BBa_I13012_sequence/1

BBa_B0010
type: Component
persistent identity: component946822
display id: component946822
version: 1
top level: BBa_I13012
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0031
type: Component
persistent identity: component946815
display id: component946815
version: 1
top level: BBa_I13012
access: public
definition: https://synbiohub.org/public/igem/BBa_B0031/1

BBa_B0012
type: Component
persistent identity: component946832
display id: component946832
version: 1
top level: BBa_I13012
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_E0030
type: Component
persistent identity: component946817
display id: component946817
version: 1
top level: BBa_I13012
access: public
definition: https://synbiohub.org/public/igem/BBa_E0030/1

BBa_R0040
type: Component
persistent identity: component946807
display id: component946807
version: 1
top level: BBa_I13012
access: public
definition: https://synbiohub.org/public/igem/BBa_R0040/1

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation946807
display id: annotation946807
version: 1
top level: BBa_I13012
location: https://synbiohub.org/public/igem/BBa_I13012/annotation946807/range946807/1
component: BBa_R0040

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type: Range
persistent identity: range946807
display id: range946807
version: 1
direction: direction/forward
top level: BBa_I13012
start: 1
end: 54
orientation: inline

BBa_E0030
type: SequenceAnnotation
persistent identity: annotation946817
display id: annotation946817
version: 1
top level: BBa_I13012
location: https://synbiohub.org/public/igem/BBa_I13012/annotation946817/range946817/1
component: BBa_E0030

https://synbiohub.org/public/igem/BBa_I13012/annotation946817/range946817/1
type: Range
persistent identity: range946817
display id: range946817
version: 1
direction: direction/forward
top level: BBa_I13012
start: 83
end: 805
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation946822
display id: annotation946822
version: 1
top level: BBa_I13012
location: https://synbiohub.org/public/igem/BBa_I13012/annotation946822/range946822/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I13012/annotation946822/range946822/1
type: Range
persistent identity: range946822
display id: range946822
version: 1
direction: direction/forward
top level: BBa_I13012
start: 814
end: 893
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation946832
display id: annotation946832
version: 1
top level: BBa_I13012
location: https://synbiohub.org/public/igem/BBa_I13012/annotation946832/range946832/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I13012/annotation946832/range946832/1
type: Range
persistent identity: range946832
display id: range946832
version: 1
direction: direction/forward
top level: BBa_I13012
start: 902
end: 942
orientation: inline

BBa_B0031
type: SequenceAnnotation
persistent identity: annotation946815
display id: annotation946815
version: 1
top level: BBa_I13012
location: https://synbiohub.org/public/igem/BBa_I13012/annotation946815/range946815/1
component: BBa_B0031

https://synbiohub.org/public/igem/BBa_I13012/annotation946815/range946815/1
type: Range
persistent identity: range946815
display id: range946815
version: 1
direction: direction/forward
top level: BBa_I13012
start: 63
end: 76
orientation: inline

BBa_I13104
type: ComponentDefinition
persistent identity: BBa I13104
display id: BBa_I13104
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Low output control (c2 QPI)
creator: Barry Canton
created: 2004-07-20T11:00:00Z
modified: 2015-08-31T04:07:33Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_6_
m user id: 0
owner id: 135
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Lac promoter upstream
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Measurement
component: BBa_R0011
component: BBa_I13020
sequence annotation: BBa_I13020
sequence annotation: BBa_R0011
sequence: https://synbiohub.org/public/igem/BBa_I13104_sequence/1

BBa_R0011
type: Component
persistent identity: component2229212
display id: component2229212
version: 1
top level: BBa_I13104
access: public
definition: https://synbiohub.org/public/igem/BBa_R0011/1

BBa_I13020
type: Component
persistent identity: component2229246
display id: component2229246
version: 1
top level: BBa_I13104
access: public
definition: https://synbiohub.org/public/igem/BBa_I13020/1

BBa_I13020
type: SequenceAnnotation
persistent identity: annotation2229246
display id: annotation2229246
version: 1
top level: BBa_I13104
location: https://synbiohub.org/public/igem/BBa_I13104/annotation2229246/range2229246/1
component: BBa_I13020

https://synbiohub.org/public/igem/BBa_I13104/annotation2229246/range2229246/1
type: Range
persistent identity: range2229246
display id: range2229246
version: 1
direction: direction/reverse
top level: BBa_I13104
start: 64
end: 1878
orientation: inline

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation2229212
display id: annotation2229212
version: 1
top level: BBa_I13104
location: https://synbiohub.org/public/igem/BBa_I13104/annotation2229212/range2229212/1
component: BBa_R0011

https://synbiohub.org/public/igem/BBa_I13104/annotation2229212/range2229212/1
type: Range
persistent identity: range2229212
display id: range2229212
version: 1
direction: direction/forward
top level: BBa_I13104
start: 1
end: 54
orientation: inline

BBa_K1379005
type: ComponentDefinition
persistent identity: BBa K1379005
display id: BBa_K1379005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: σx Generator + PcelA-E0240
creator: Jordy Evan, Nadia Benedicta, Angeline Widjaja
created: 2014-09-22T11:00:00Z
modified: 2015-05-08T01:10:12Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1756_
m user id: 0
owner id: 21235
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: SigmaX generator, SigmaX inducible PCelA encoding GFP
mutable notes: N/A
mutable provenance: Both comX gene and PCelA are found in genomic sequence of Streptococcus pneumoniae NCTC7465 strain, while the RBS+GFP+DTT which is downstream of PCelA is BBa_E0240. The promoter encoding comX is BBa_K880005 and the double terminator for it is BBa_B0015
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Measurement
component: BBa_K1379006
component: BBa_E0240
component: BBa_K1379000
sequence annotation: BBa_K1379000
sequence annotation: BBa_K1379006
sequence annotation: BBa_E0240
sequence: https://synbiohub.org/public/igem/BBa_K1379005_sequence/1

BBa_K1379006
type: Component
persistent identity: component2407436
display id: component2407436
version: 1
top level: BBa_K1379005
access: public
definition: https://synbiohub.org/public/igem/BBa_K1379006/1

BBa_E0240
type: Component
persistent identity: component2407450
display id: component2407450
version: 1
top level: BBa_K1379005
access: public
definition: https://synbiohub.org/public/igem/BBa_E0240/1

BBa_K1379000
type: Component
persistent identity: component2407439
display id: component2407439
version: 1
top level: BBa_K1379005
access: public
definition: https://synbiohub.org/public/igem/BBa_K1379000/1

BBa_K1379000
type: SequenceAnnotation
persistent identity: annotation2407439
display id: annotation2407439
version: 1
top level: BBa_K1379005
location: https://synbiohub.org/public/igem/BBa_K1379005/annotation2407439/range2407439/1
component: BBa_K1379000

https://synbiohub.org/public/igem/BBa_K1379005/annotation2407439/range2407439/1
type: Range
persistent identity: range2407439
display id: range2407439
version: 1
direction: direction/reverse
top level: BBa_K1379005
start: 720
end: 819
orientation: inline

BBa_K1379006
type: SequenceAnnotation
persistent identity: annotation2407436
display id: annotation2407436
version: 1
top level: BBa_K1379005
location: https://synbiohub.org/public/igem/BBa_K1379005/annotation2407436/range2407436/1
component: BBa_K1379006

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type: Range
persistent identity: range2407436
display id: range2407436
version: 1
direction: direction/reverse
top level: BBa_K1379005
start: 1
end: 711
orientation: inline

BBa_E0240
type: SequenceAnnotation
persistent identity: annotation2407450
display id: annotation2407450
version: 1
top level: BBa_K1379005
location: https://synbiohub.org/public/igem/BBa_K1379005/annotation2407450/range2407450/1
component: BBa_E0240

https://synbiohub.org/public/igem/BBa_K1379005/annotation2407450/range2407450/1
type: Range
persistent identity: range2407450
display id: range2407450
version: 1
direction: direction/reverse
top level: BBa_K1379005
start: 828
end: 1703
orientation: inline

BBa_I13112
type: ComponentDefinition
persistent identity: BBa I13112
display id: BBa_I13112
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: High Output control (c1 QPI)
creator: Barry Canton
created: 2004-07-20T11:00:00Z
modified: 2015-08-31T04:07:33Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_6_
m user id: 0
owner id: 135
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Upstream terminator, no promoter
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Measurement
component: BBa_B0012
component: BBa_B0034
component: BBa_C0051
component: BBa_B0010
component: BBa_E0030
component: BBa_R0051
sequence annotation: BBa_B0012
sequence annotation: BBa_B0034
sequence annotation: BBa_B0010
sequence annotation: BBa_C0051
sequence annotation: BBa_R0051
sequence annotation: BBa_E0030
sequence: https://synbiohub.org/public/igem/BBa_I13112_sequence/1

BBa_B0012
type: Component
persistent identity: component971881
display id: component971881
version: 1
top level: BBa_I13112
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0034
type: Component
persistent identity: component971815
display id: component971815
version: 1
top level: BBa_I13112
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_C0051
type: Component
persistent identity: component971825
display id: component971825
version: 1
top level: BBa_I13112
access: public
definition: https://synbiohub.org/public/igem/BBa_C0051/1

BBa_B0010
type: Component
persistent identity: component971871
display id: component971871
version: 1
top level: BBa_I13112
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0010
type: Component
persistent identity: component971797
display id: component971797
version: 1
top level: BBa_I13112
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0034
type: Component
persistent identity: component971864
display id: component971864
version: 1
top level: BBa_I13112
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0010
type: Component
persistent identity: component971831
display id: component971831
version: 1
top level: BBa_I13112
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_E0030
type: Component
persistent identity: component971866
display id: component971866
version: 1
top level: BBa_I13112
access: public
definition: https://synbiohub.org/public/igem/BBa_E0030/1

BBa_B0012
type: Component
persistent identity: component971807
display id: component971807
version: 1
top level: BBa_I13112
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0012
type: Component
persistent identity: component971841
display id: component971841
version: 1
top level: BBa_I13112
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_R0051
type: Component
persistent identity: component971856
display id: component971856
version: 1
top level: BBa_I13112
access: public
definition: https://synbiohub.org/public/igem/BBa_R0051/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation971841
display id: annotation971841
version: 1
top level: BBa_I13112
location: https://synbiohub.org/public/igem/BBa_I13112/annotation971841/range971841/1
component: BBa_B0012

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type: Range
persistent identity: range971841
display id: range971841
version: 1
direction: direction/forward
top level: BBa_I13112
start: 1027
end: 1067
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation971864
display id: annotation971864
version: 1
top level: BBa_I13112
location: https://synbiohub.org/public/igem/BBa_I13112/annotation971864/range971864/1
component: BBa_B0034

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type: Range
persistent identity: range971864
display id: range971864
version: 1
direction: direction/forward
top level: BBa_I13112
start: 1133
end: 1144
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation971871
display id: annotation971871
version: 1
top level: BBa_I13112
location: https://synbiohub.org/public/igem/BBa_I13112/annotation971871/range971871/1
component: BBa_B0010

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type: Range
persistent identity: range971871
display id: range971871
version: 1
direction: direction/forward
top level: BBa_I13112
start: 1882
end: 1961
orientation: inline

BBa_C0051
type: SequenceAnnotation
persistent identity: annotation971825
display id: annotation971825
version: 1
top level: BBa_I13112
location: https://synbiohub.org/public/igem/BBa_I13112/annotation971825/range971825/1
component: BBa_C0051

https://synbiohub.org/public/igem/BBa_I13112/annotation971825/range971825/1
type: Range
persistent identity: range971825
display id: range971825
version: 1
direction: direction/forward
top level: BBa_I13112
start: 156
end: 905
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation971807
display id: annotation971807
version: 1
top level: BBa_I13112
location: https://synbiohub.org/public/igem/BBa_I13112/annotation971807/range971807/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I13112/annotation971807/range971807/1
type: Range
persistent identity: range971807
display id: range971807
version: 1
direction: direction/forward
top level: BBa_I13112
start: 89
end: 129
orientation: inline

BBa_R0051
type: SequenceAnnotation
persistent identity: annotation971856
display id: annotation971856
version: 1
top level: BBa_I13112
location: https://synbiohub.org/public/igem/BBa_I13112/annotation971856/range971856/1
component: BBa_R0051

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BBa_B0010
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description: R0010.E0021.B0022.E0031
creator: Caitlin Conboy
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modified: 2015-08-31T04:07:45Z
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dominant: false
group u list: _1_
m user id: 0
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sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Terminator Characterzation Construct
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
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was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Cascaded Tet (medium) and Lac (medium) QPIs with double terminator as input
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created: 2004-07-19T11:00:00Z
modified: 2015-08-31T04:07:36Z
discontinued: false
dominant: false
experience: experience/None
group u list: _6_
m user id: 0
owner id: 101
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
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component: BBa_Q01121
component: BBa_Q01400
component: BBa_B0015
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definition: https://synbiohub.org/public/igem/BBa_Q01121/1

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type: ComponentDefinition
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version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Cascaded Tet (medium) and Lac (weak) QPIs
creator: mit
created: 2004-07-19T11:00:00Z
modified: 2015-08-31T04:07:36Z
discontinued: false
dominant: false
experience: experience/None
group u list: _6_
m user id: 0
owner id: 101
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: false
type: DnaRegion
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component: BBa_Q03121
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BBa_Q03121
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type: ComponentDefinition
persistent identity: BBa I13512
display id: BBa_I13512
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Screening plasmid 1
creator: jkm
created: 2005-05-30T11:00:00Z
modified: 2015-08-31T04:07:34Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_
m user id: 0
owner id: 253
owning group id: 6
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: The first screening plasmid. Ends will be capped with I13450. Cutting I13452 generates an internal BioBrick site.
owned by: james
owned by: myers
star: false
type: DnaRegion
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role: partType/Measurement
component: BBa_B0032
component: BBa_E1010
component: BBa_E0040
component: BBa_B0012
component: BBa_B0010
component: BBa_I0500
component: BBa_I13452
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version: 1
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version: 1
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version: 1
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definition: https://synbiohub.org/public/igem/BBa_I13452/1

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type: ComponentDefinition
persistent identity: BBa I13627
display id: BBa_I13627
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Cascaded Lac (elowitz) and Tet (medium) QPIs
creator: mit
created: 2004-07-19T11:00:00Z
modified: 2015-08-31T04:07:36Z
discontinued: false
dominant: false
experience: experience/None
group u list: _6_
m user id: 0
owner id: 101
owning group id: 7
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Measurement
component: BBa_Q01400
component: BBa_Q04121
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top level: BBa_I13627
access: public
definition: https://synbiohub.org/public/igem/BBa_Q01400/1

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access: public
definition: https://synbiohub.org/public/igem/BBa_Q04121/1

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version: 1
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version: 1
direction: direction/reverse
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BBa_I0413
type: ComponentDefinition
persistent identity: BBa I0413
display id: BBa_I0413
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS Test R0040.B0030.E0030.B0015
creator: jcbraff
created: 2004-06-13T11:00:00Z
modified: 2015-08-31T04:07:28Z
discontinued: false
dominant: true
experience: experience/None
group u list: _11_1_
m user id: 0
owner id: 61
owning group id: 7
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: R0040.B0030.E0030.B0015 RBS Test
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Measurement
component: BBa_B0012
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sequence annotation: BBa_B0030
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definition: https://synbiohub.org/public/igem/BBa_B0010/1

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version: 1
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BBa_K518013
type: ComponentDefinition
persistent identity: BBa K518013
display id: BBa_K518013
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: sulA promoter evaluation device
creator: Masato Ohgishi
created: 2011-10-02T11:00:00Z
modified: 2015-05-08T01:12:33Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _683_
m user id: 0
owner id: 8305
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: Microbes including Escherichia coli are known to respond to various DNA-injuring stress (ionizing radiation, ultraviolet radiation, peroxides etc...), altering their gene expressions. This response, known as "SOS response", are induced by regulatory protein called RecA, which is activated when binds to single-strand DNA. DNA-RecA complex promotes the self-degradation of LexA, a common repressor for SOS genes. SulA is responsible for stress-induced halt of cell division. The promoter of sulA, or sulAp, is acutely induced by various stress factors, including ultraviolet irradiation. We provide a sulAp evaluation device to make it easy to measure the varying expression of sulAp in various "stressful" conditions. Employing a dual luciferase reporter construct, quantitative measurement and comparison of Relative Promoter Unit (RPU) is achieved.
mutable notes: sulAp + dual luciferase reporter construct
mutable provenance: sulAp (BBa_K518010) was cloned from Escherichia coli K12 strain's genome.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Measurement
component: BBa_K518010
component: BBa_K518002
sequence annotation: BBa_K518010
sequence annotation: BBa_K518002
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display id: component2151395
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access: public
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display id: component2151421
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definition: BBa_K518002

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BBa_I6310
type: ComponentDefinition
persistent identity: BBa I6310
display id: BBa_I6310
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: QPI Curve Test I0500.E0420.I0500.Q04500.E0430
creator: cconboy
created: 2004-05-09T11:00:00Z
modified: 2015-08-31T04:07:44Z
discontinued: true
dominant: false
experience: experience/None
group u list: _11_1_
m user id: 0
owner id: 60
owning group id: 7
sample status: Discontinued
status: status/Deleted
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Measurement
component: BBa_B0034
component: BBa_B0012
component: BBa_B0010
component: BBa_I0500
component: BBa_E0020
component: BBa_R0050
component: BBa_C0050
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sequence annotation: BBa_B0034
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sequence annotation: BBa_I0500
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access: public
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definition: https://synbiohub.org/public/igem/BBa_B0010/1

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version: 1
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definition: https://synbiohub.org/public/igem/BBa_B0012/1

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display id: range980135
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BBa_B0010
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display id: annotation980221
version: 1
top level: BBa_I6310
location: https://synbiohub.org/public/igem/BBa_I6310/annotation980221/range980221/1
component: BBa_B0010

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type: Range
persistent identity: range980221
display id: range980221
version: 1
direction: direction/forward
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start: 5067
end: 5146
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation980214
display id: annotation980214
version: 1
top level: BBa_I6310
location: https://synbiohub.org/public/igem/BBa_I6310/annotation980214/range980214/1
component: BBa_B0034

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display id: range980214
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BBa_B0012
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display id: annotation980128
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display id: range980128
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persistent identity: annotation980188
display id: annotation980188
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component: BBa_B0012

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persistent identity: range980188
display id: range980188
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BBa_C0050
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display id: annotation980170
version: 1
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location: https://synbiohub.org/public/igem/BBa_I6310/annotation980170/range980170/1
component: BBa_C0050

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persistent identity: range980170
display id: range980170
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BBa_B0010
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display id: annotation980178
version: 1
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location: https://synbiohub.org/public/igem/BBa_I6310/annotation980178/range980178/1
component: BBa_B0010

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persistent identity: range980178
display id: range980178
version: 1
direction: direction/forward
top level: BBa_I6310
start: 4118
end: 4197
orientation: inline

BBa_E0020
type: SequenceAnnotation
persistent identity: annotation980130
display id: annotation980130
version: 1
top level: BBa_I6310
location: https://synbiohub.org/public/igem/BBa_I6310/annotation980130/range980130/1
component: BBa_E0020

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display id: range980130
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BBa_R0050
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display id: annotation980231
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persistent identity: annotation980150
display id: annotation980150
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BBa_I7108
type: ComponentDefinition
persistent identity: BBa I7108
display id: BBa_I7108
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: R0053.E0840
creator: Jennifer Braff
created: 2005-07-27T11:00:00Z
modified: 2015-08-31T04:07:45Z
discontinued: false
dominant: false
group u list: _11_
m user id: 0
owner id: 61
owning group id: 1
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: R0053.E0840 (S_N; strong rbs; low PoPS)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Measurement
component: BBa_E0040
component: BBa_B0010
component: BBa_B0012
component: BBa_B0030
component: BBa_R0053
sequence annotation: BBa_B0010
sequence annotation: BBa_B0030
sequence annotation: BBa_B0012
sequence annotation: BBa_R0053
sequence annotation: BBa_E0040
sequence: https://synbiohub.org/public/igem/BBa_I7108_sequence/1

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BBa_B0010
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BBa_B0012
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BBa_I6116
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display id: BBa_I6116
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was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: R0077.E0030.B0015
creator: jcbraff
created: 2004-06-08T11:00:00Z
modified: 2015-08-31T04:07:44Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_1_
m user id: 0
owner id: 61
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Measurement
component: BBa_B0012
component: BBa_R0077
component: BBa_E0030
component: BBa_B0010
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sequence annotation: BBa_R0077
sequence annotation: BBa_B0010
sequence annotation: BBa_E0030
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BBa_B0012
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BBa_B0010
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access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0012
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BBa_K389013
type: ComponentDefinition
persistent identity: BBa K389013
display id: BBa_K389013
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: VirA reporter system mRFP
creator: Jonas Aretz
created: 2010-09-10T11:00:00Z
modified: 2015-05-08T01:12:19Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _517_
m user id: 0
owner id: 6670
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: With this system it is possible to measure the activity of a virA receptor and the vir promoter, respectively. The reporter is the mRFP from the partsregistry.
mutable notes: medium strong constitutive promotor, terminator to keep basal expression of mRFP low
mutable provenance: partsregistry, synthetic gene by Mr. Gene, TI-plasmid from ''Agrobacterium tumefaciens'' C58
owned by: james
owned by: myers
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sequence annotation: BBa_K389002
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sequence annotation: BBa_E1010
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BBa_I5131
type: ComponentDefinition
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display id: BBa_I5131
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: R0053.B0034.E0030.B0014
creator: Reshma Shetty
created: 2005-05-12T11:00:00Z
modified: 2015-08-31T04:07:42Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _6_
m user id: 0
owner id: 126
owning group id: 6
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: false
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BBa_R0053
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BBa_B0034
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BBa_R0053
type: SequenceAnnotation
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location: https://synbiohub.org/public/igem/BBa_I5131/annotation1487691/range1487691/1
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BBa_I13108
type: ComponentDefinition
persistent identity: BBa I13108
display id: BBa_I13108
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Low output control (c1 QPI, Elowitz RBS)
creator: Barry Canton
created: 2004-07-20T11:00:00Z
modified: 2015-08-31T04:07:33Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_6_
m user id: 0
owner id: 135
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: tet promoter upstream
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Measurement
component: BBa_B0034
component: BBa_B0010
component: BBa_B0012
component: BBa_C0051
component: BBa_E0030
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component: BBa_R0040
sequence annotation: BBa_B0012
sequence annotation: BBa_R0040
sequence annotation: BBa_B0010
sequence annotation: BBa_E0030
sequence annotation: BBa_B0034
sequence annotation: BBa_R0051
sequence annotation: BBa_C0051
sequence: https://synbiohub.org/public/igem/BBa_I13108_sequence/1

BBa_B0034
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BBa_B0010
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BBa_B0034
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definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0010
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version: 1
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definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0012
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version: 1
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definition: https://synbiohub.org/public/igem/BBa_B0012/1

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definition: https://synbiohub.org/public/igem/BBa_C0051/1

BBa_E0030
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BBa_R0051
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definition: https://synbiohub.org/public/igem/BBa_R0051/1

BBa_B0012
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definition: https://synbiohub.org/public/igem/BBa_R0040/1

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BBa_C0051
type: SequenceAnnotation
persistent identity: annotation966197
display id: annotation966197
version: 1
top level: BBa_I13108
location: https://synbiohub.org/public/igem/BBa_I13108/annotation966197/range966197/1
component: BBa_C0051

https://synbiohub.org/public/igem/BBa_I13108/annotation966197/range966197/1
type: Range
persistent identity: range966197
display id: range966197
version: 1
direction: direction/forward
top level: BBa_I13108
start: 81
end: 830
orientation: inline

BBa_I7107
type: ComponentDefinition
persistent identity: BBa I7107
display id: BBa_I7107
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: R0011.E0240
creator: Jennifer Braff
created: 2005-07-27T11:00:00Z
modified: 2015-08-31T04:07:45Z
discontinued: false
dominant: true
group u list: _11_
m user id: 0
owner id: 61
owning group id: 1
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: R0011.E0240 (S_N; med. rbs; high PoPS)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Measurement
component: BBa_R0011
component: BBa_B0032
component: BBa_B0010
component: BBa_B0012
component: BBa_E0040
sequence annotation: BBa_R0011
sequence annotation: BBa_E0040
sequence annotation: BBa_B0012
sequence annotation: BBa_B0032
sequence annotation: BBa_B0010
sequence: https://synbiohub.org/public/igem/BBa_I7107_sequence/1

BBa_R0011
type: Component
persistent identity: component1596615
display id: component1596615
version: 1
top level: BBa_I7107
access: public
definition: https://synbiohub.org/public/igem/BBa_R0011/1

BBa_B0032
type: Component
persistent identity: component1596627
display id: component1596627
version: 1
top level: BBa_I7107
access: public
definition: https://synbiohub.org/public/igem/BBa_B0032/1

BBa_B0010
type: Component
persistent identity: component1596635
display id: component1596635
version: 1
top level: BBa_I7107
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0012
type: Component
persistent identity: component1596645
display id: component1596645
version: 1
top level: BBa_I7107
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_E0040
type: Component
persistent identity: component1596630
display id: component1596630
version: 1
top level: BBa_I7107
access: public
definition: https://synbiohub.org/public/igem/BBa_E0040/1

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation1596615
display id: annotation1596615
version: 1
top level: BBa_I7107
location: https://synbiohub.org/public/igem/BBa_I7107/annotation1596615/range1596615/1
component: BBa_R0011

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type: Range
persistent identity: range1596615
display id: range1596615
version: 1
direction: direction/forward
top level: BBa_I7107
start: 1
end: 54
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation1596630
display id: annotation1596630
version: 1
top level: BBa_I7107
location: https://synbiohub.org/public/igem/BBa_I7107/annotation1596630/range1596630/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_I7107/annotation1596630/range1596630/1
type: Range
persistent identity: range1596630
display id: range1596630
version: 1
direction: direction/forward
top level: BBa_I7107
start: 83
end: 802
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1596645
display id: annotation1596645
version: 1
top level: BBa_I7107
location: https://synbiohub.org/public/igem/BBa_I7107/annotation1596645/range1596645/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I7107/annotation1596645/range1596645/1
type: Range
persistent identity: range1596645
display id: range1596645
version: 1
direction: direction/forward
top level: BBa_I7107
start: 899
end: 939
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation1596627
display id: annotation1596627
version: 1
top level: BBa_I7107
location: https://synbiohub.org/public/igem/BBa_I7107/annotation1596627/range1596627/1
component: BBa_B0032

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type: Range
persistent identity: range1596627
display id: range1596627
version: 1
direction: direction/forward
top level: BBa_I7107
start: 64
end: 76
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1596635
display id: annotation1596635
version: 1
top level: BBa_I7107
location: https://synbiohub.org/public/igem/BBa_I7107/annotation1596635/range1596635/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I7107/annotation1596635/range1596635/1
type: Range
persistent identity: range1596635
display id: range1596635
version: 1
direction: direction/forward
top level: BBa_I7107
start: 811
end: 890
orientation: inline

BBa_I6316
type: ComponentDefinition
persistent identity: BBa I6316
display id: BBa_I6316
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: QPI Curve Test I0500.E0420.I0500.Q04511.E0430
creator: cconboy
created: 2004-05-09T11:00:00Z
modified: 2015-08-31T04:07:44Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_1_
m user id: 0
owner id: 60
owning group id: 7
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000804
role: partType/Measurement
component: BBa_R0065
component: BBa_I0500
component: BBa_C0051
component: BBa_B0012
component: BBa_B0010
component: BBa_E0020
component: BBa_B0034
component: BBa_E0030
sequence annotation: BBa_C0051
sequence annotation: BBa_B0034
sequence annotation: BBa_B0012
sequence annotation: BBa_E0020
sequence annotation: BBa_E0030
sequence annotation: BBa_B0010
sequence annotation: BBa_I0500
sequence annotation: BBa_R0065
sequence: https://synbiohub.org/public/igem/BBa_I6316_sequence/1

BBa_R0065
type: Component
persistent identity: component980415
display id: component980415
version: 1
top level: BBa_I6316
access: public
definition: https://synbiohub.org/public/igem/BBa_R0065/1

BBa_I0500
type: Component
persistent identity: component980364
display id: component980364
version: 1
top level: BBa_I6316
access: public
definition: https://synbiohub.org/public/igem/BBa_I0500/1

BBa_C0051
type: Component
persistent identity: component980379
display id: component980379
version: 1
top level: BBa_I6316
access: public
definition: https://synbiohub.org/public/igem/BBa_C0051/1

BBa_B0012
type: Component
persistent identity: component980359
display id: component980359
version: 1
top level: BBa_I6316
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0010
type: Component
persistent identity: component980434
display id: component980434
version: 1
top level: BBa_I6316
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_I0500
type: Component
persistent identity: component980337
display id: component980337
version: 1
top level: BBa_I6316
access: public
definition: https://synbiohub.org/public/igem/BBa_I0500/1

BBa_B0010
type: Component
persistent identity: component980385
display id: component980385
version: 1
top level: BBa_I6316
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0012
type: Component
persistent identity: component980444
display id: component980444
version: 1
top level: BBa_I6316
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_E0020
type: Component
persistent identity: component980344
display id: component980344
version: 1
top level: BBa_I6316
access: public
definition: https://synbiohub.org/public/igem/BBa_E0020/1

BBa_B0010
type: Component
persistent identity: component980349
display id: component980349
version: 1
top level: BBa_I6316
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0034
type: Component
persistent identity: component980342
display id: component980342
version: 1
top level: BBa_I6316
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0034
type: Component
persistent identity: component980427
display id: component980427
version: 1
top level: BBa_I6316
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0034
type: Component
persistent identity: component980369
display id: component980369
version: 1
top level: BBa_I6316
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0012
type: Component
persistent identity: component980395
display id: component980395
version: 1
top level: BBa_I6316
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_E0030
type: Component
persistent identity: component980429
display id: component980429
version: 1
top level: BBa_I6316
access: public
definition: https://synbiohub.org/public/igem/BBa_E0030/1

BBa_C0051
type: SequenceAnnotation
persistent identity: annotation980379
display id: annotation980379
version: 1
top level: BBa_I6316
location: https://synbiohub.org/public/igem/BBa_I6316/annotation980379/range980379/1
component: BBa_C0051

https://synbiohub.org/public/igem/BBa_I6316/annotation980379/range980379/1
type: Range
persistent identity: range980379
display id: range980379
version: 1
direction: direction/forward
top level: BBa_I6316
start: 3341
end: 4090
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation980427
display id: annotation980427
version: 1
top level: BBa_I6316
location: https://synbiohub.org/public/igem/BBa_I6316/annotation980427/range980427/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I6316/annotation980427/range980427/1
type: Range
persistent identity: range980427
display id: range980427
version: 1
direction: direction/forward
top level: BBa_I6316
start: 4366
end: 4377
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation980444
display id: annotation980444
version: 1
top level: BBa_I6316
location: https://synbiohub.org/public/igem/BBa_I6316/annotation980444/range980444/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I6316/annotation980444/range980444/1
type: Range
persistent identity: range980444
display id: range980444
version: 1
direction: direction/forward
top level: BBa_I6316
start: 5203
end: 5243
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation980359
display id: annotation980359
version: 1
top level: BBa_I6316
location: https://synbiohub.org/public/igem/BBa_I6316/annotation980359/range980359/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I6316/annotation980359/range980359/1
type: Range
persistent identity: range980359
display id: range980359
version: 1
direction: direction/forward
top level: BBa_I6316
start: 2056
end: 2096
orientation: inline

BBa_E0020
type: SequenceAnnotation
persistent identity: annotation980344
display id: annotation980344
version: 1
top level: BBa_I6316
location: https://synbiohub.org/public/igem/BBa_I6316/annotation980344/range980344/1
component: BBa_E0020

https://synbiohub.org/public/igem/BBa_I6316/annotation980344/range980344/1
type: Range
persistent identity: range980344
display id: range980344
version: 1
direction: direction/forward
top level: BBa_I6316
start: 1237
end: 1959
orientation: inline

BBa_E0030
type: SequenceAnnotation
persistent identity: annotation980429
display id: annotation980429
version: 1
top level: BBa_I6316
location: https://synbiohub.org/public/igem/BBa_I6316/annotation980429/range980429/1
component: BBa_E0030

https://synbiohub.org/public/igem/BBa_I6316/annotation980429/range980429/1
type: Range
persistent identity: range980429
display id: range980429
version: 1
direction: direction/forward
top level: BBa_I6316
start: 4384
end: 5106
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation980349
display id: annotation980349
version: 1
top level: BBa_I6316
location: https://synbiohub.org/public/igem/BBa_I6316/annotation980349/range980349/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I6316/annotation980349/range980349/1
type: Range
persistent identity: range980349
display id: range980349
version: 1
direction: direction/forward
top level: BBa_I6316
start: 1968
end: 2047
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation980434
display id: annotation980434
version: 1
top level: BBa_I6316
location: https://synbiohub.org/public/igem/BBa_I6316/annotation980434/range980434/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I6316/annotation980434/range980434/1
type: Range
persistent identity: range980434
display id: range980434
version: 1
direction: direction/forward
top level: BBa_I6316
start: 5115
end: 5194
orientation: inline

BBa_I0500
type: SequenceAnnotation
persistent identity: annotation980364
display id: annotation980364
version: 1
top level: BBa_I6316
location: https://synbiohub.org/public/igem/BBa_I6316/annotation980364/range980364/1
component: BBa_I0500

https://synbiohub.org/public/igem/BBa_I6316/annotation980364/range980364/1
type: Range
persistent identity: range980364
display id: range980364
version: 1
direction: direction/forward
top level: BBa_I6316
start: 2105
end: 3314
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation980395
display id: annotation980395
version: 1
top level: BBa_I6316
location: https://synbiohub.org/public/igem/BBa_I6316/annotation980395/range980395/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I6316/annotation980395/range980395/1
type: Range
persistent identity: range980395
display id: range980395
version: 1
direction: direction/forward
top level: BBa_I6316
start: 4212
end: 4252
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation980385
display id: annotation980385
version: 1
top level: BBa_I6316
location: https://synbiohub.org/public/igem/BBa_I6316/annotation980385/range980385/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I6316/annotation980385/range980385/1
type: Range
persistent identity: range980385
display id: range980385
version: 1
direction: direction/forward
top level: BBa_I6316
start: 4124
end: 4203
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation980369
display id: annotation980369
version: 1
top level: BBa_I6316
location: https://synbiohub.org/public/igem/BBa_I6316/annotation980369/range980369/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I6316/annotation980369/range980369/1
type: Range
persistent identity: range980369
display id: range980369
version: 1
direction: direction/forward
top level: BBa_I6316
start: 3323
end: 3334
orientation: inline

BBa_I0500
type: SequenceAnnotation
persistent identity: annotation980337
display id: annotation980337
version: 1
top level: BBa_I6316
location: https://synbiohub.org/public/igem/BBa_I6316/annotation980337/range980337/1
component: BBa_I0500

https://synbiohub.org/public/igem/BBa_I6316/annotation980337/range980337/1
type: Range
persistent identity: range980337
display id: range980337
version: 1
direction: direction/forward
top level: BBa_I6316
start: 1
end: 1210
orientation: inline

BBa_R0065
type: SequenceAnnotation
persistent identity: annotation980415
display id: annotation980415
version: 1
top level: BBa_I6316
location: https://synbiohub.org/public/igem/BBa_I6316/annotation980415/range980415/1
component: BBa_R0065

https://synbiohub.org/public/igem/BBa_I6316/annotation980415/range980415/1
type: Range
persistent identity: range980415
display id: range980415
version: 1
direction: direction/forward
top level: BBa_I6316
start: 4261
end: 4357
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation980342
display id: annotation980342
version: 1
top level: BBa_I6316
location: https://synbiohub.org/public/igem/BBa_I6316/annotation980342/range980342/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I6316/annotation980342/range980342/1
type: Range
persistent identity: range980342
display id: range980342
version: 1
direction: direction/forward
top level: BBa_I6316
start: 1219
end: 1230
orientation: inline

BBa_K772008
type: ComponentDefinition
persistent identity: BBa K772008
display id: BBa_K772008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: GFP - LacZ protein complex with flag tags
creator: Mustafa Elitok
created: 2012-09-25T11:00:00Z
modified: 2015-05-08T01:13:15Z
discontinued: false
dominant: false
group u list: _1024_
m user id: 0
owner id: 9573
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This part contains a green fluorescent protein (GFP) as a non-reactive chemical compound (anchor) in order to neutralize the active reactive fixed to it, a reporter device (LacZ enzyme catalyses the reaction of the cleavage of X-Gal which results with blue color change) and a TEV protease cleavage site between them to assemble these two proteins. BBa_772001 and BBa_K772002 are assembled to be used as the trigger of detection in the whole system which ends with the intracellular transfection (or activation) of TEV protease. After the activation, it is planned that TEV protease will cleave this anchor-inducer protein complex to release free the reporter. In conclusion, in this system, the inducer would activate a transcription pathway on the targeted inducible gene or the reporter would indicate that there is a change in the whole system. This part contains flag tags extra from BBa_K772006 in the GFP region; because it is planned to be used in the Co-IP/SDS-PAGE assay to be isolated.
mutable notes: Cleavage site, LacZ and GFP sequences are gathered from Registry.
mutable provenance: Note that there is an RBS sequence just before the protein sequence in order to eliminate the need of addition of it.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Measurement
sequence annotation: Flag Tag
sequence annotation: TEV cleavage site
sequence annotation: Double Terminator
sequence annotation: GFP
sequence annotation: Anchor-Reporter + Flag
sequence annotation: RBS
sequence annotation: LacZ
sequence: https://synbiohub.org/public/igem/BBa_K772008_sequence/1

Flag Tag
type: SequenceAnnotation
persistent identity: annotation2203911
display id: annotation2203911
version: 1
top level: BBa_K772008
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K772008/annotation2203911/range2203911/1

https://synbiohub.org/public/igem/BBa_K772008/annotation2203911/range2203911/1
type: Range
persistent identity: range2203911
display id: range2203911
version: 1
direction: direction/forward
top level: BBa_K772008
start: 21
end: 46
orientation: inline

TEV cleavage site
type: SequenceAnnotation
persistent identity: annotation2203913
display id: annotation2203913
version: 1
top level: BBa_K772008
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K772008/annotation2203913/range2203913/1

https://synbiohub.org/public/igem/BBa_K772008/annotation2203913/range2203913/1
type: Range
persistent identity: range2203913
display id: range2203913
version: 1
direction: direction/forward
top level: BBa_K772008
start: 768
end: 857
orientation: inline

Double Terminator
type: SequenceAnnotation
persistent identity: annotation2203915
display id: annotation2203915
version: 1
top level: BBa_K772008
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K772008/annotation2203915/range2203915/1

https://synbiohub.org/public/igem/BBa_K772008/annotation2203915/range2203915/1
type: Range
persistent identity: range2203915
display id: range2203915
version: 1
direction: direction/forward
top level: BBa_K772008
start: 1102
end: 1232
orientation: inline

GFP
type: SequenceAnnotation
persistent identity: annotation2203912
display id: annotation2203912
version: 1
top level: BBa_K772008
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K772008/annotation2203912/range2203912/1

https://synbiohub.org/public/igem/BBa_K772008/annotation2203912/range2203912/1
type: Range
persistent identity: range2203912
display id: range2203912
version: 1
direction: direction/forward
top level: BBa_K772008
start: 47
end: 767
orientation: inline

Anchor-Reporter + Flag
type: SequenceAnnotation
persistent identity: annotation2203916
display id: annotation2203916
version: 1
top level: BBa_K772008
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K772008/annotation2203916/range2203916/1

https://synbiohub.org/public/igem/BBa_K772008/annotation2203916/range2203916/1
type: Range
persistent identity: range2203916
display id: range2203916
version: 1
direction: direction/forward
top level: BBa_K772008
start: 21
end: 1101
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2203910
display id: annotation2203910
version: 1
top level: BBa_K772008
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K772008/annotation2203910/range2203910/1

https://synbiohub.org/public/igem/BBa_K772008/annotation2203910/range2203910/1
type: Range
persistent identity: range2203910
display id: range2203910
version: 1
direction: direction/forward
top level: BBa_K772008
start: 1
end: 20
orientation: inline

LacZ
type: SequenceAnnotation
persistent identity: annotation2203914
display id: annotation2203914
version: 1
top level: BBa_K772008
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K772008/annotation2203914/range2203914/1

https://synbiohub.org/public/igem/BBa_K772008/annotation2203914/range2203914/1
type: Range
persistent identity: range2203914
display id: range2203914
version: 1
direction: direction/forward
top level: BBa_K772008
start: 858
end: 1101
orientation: inline

BBa_I13633
type: ComponentDefinition
persistent identity: BBa I13633
display id: BBa_I13633
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Cascaded Tet (medium) and Lac (weak) QPIs with double terminator as input
creator: mit
created: 2004-07-19T11:00:00Z
modified: 2015-08-31T04:07:36Z
discontinued: false
dominant: false
experience: experience/None
group u list: _6_
m user id: 0
owner id: 101
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Measurement
component: BBa_Q03121
component: BBa_Q01400
component: BBa_B0015
sequence annotation: BBa_Q03121
sequence annotation: BBa_Q01400
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_I13633_sequence/1

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access: public
definition: https://synbiohub.org/public/igem/BBa_Q01400/1

BBa_B0015
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display id: component2232254
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access: public
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BBa_Q03121
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BBa_I13657
type: ComponentDefinition
persistent identity: BBa I13657
display id: BBa_I13657
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Cascaded Tet (elowitz) and Lac (medium) QPIs with constitutive promoter as input
creator: mit
created: 2004-07-19T11:00:00Z
modified: 2015-08-31T04:07:36Z
discontinued: false
dominant: false
experience: experience/None
group u list: _6_
m user id: 0
owner id: 101
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Measurement
component: BBa_Q01121
component: BBa_Q04400
component: BBa_R0051
sequence annotation: BBa_Q04400
sequence annotation: BBa_Q01121
sequence annotation: BBa_R0051
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BBa_I13611
type: ComponentDefinition
persistent identity: BBa I13611
display id: BBa_I13611
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Cascaded Tet (weak) and Lac (medium) QPIs
creator: mit
created: 2004-07-19T11:00:00Z
modified: 2015-08-31T04:07:36Z
discontinued: false
dominant: false
experience: experience/None
group u list: _6_
m user id: 0
owner id: 101
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Measurement
component: BBa_Q03400
component: BBa_Q01121
sequence annotation: BBa_Q01121
sequence annotation: BBa_Q03400
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access: public
definition: https://synbiohub.org/public/igem/BBa_Q03400/1

BBa_Q01121
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access: public
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component: BBa_Q03400

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BBa_I13513
type: ComponentDefinition
persistent identity: BBa I13513
display id: BBa_I13513
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Screening Plasmid 2
creator: jkm
created: 2005-05-30T11:00:00Z
modified: 2015-08-31T04:07:34Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _11_
m user id: 0
owner id: 253
owning group id: 6
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: The second screening plasmid. Same as the first, except with stronger RBSs. Ends should be capped with I13450.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Measurement
component: BBa_I0500
component: BBa_B0012
component: BBa_B0034
component: BBa_B0010
component: BBa_I13452
component: BBa_E1010
component: BBa_E0040
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sequence annotation: BBa_B0034
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sequence annotation: BBa_B0010
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BBa_I0500
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BBa_B0012
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BBa_B0034
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version: 1
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access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0010
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display id: component1505351
version: 1
top level: BBa_I13513
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_I13452
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BBa_B0034
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BBa_E1010
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access: public
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BBa_E0040
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display id: component1505346
version: 1
top level: BBa_I13513
access: public
definition: https://synbiohub.org/public/igem/BBa_E0040/1

BBa_I13452
type: SequenceAnnotation
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orientation: inline

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orientation: inline

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BBa_B0034
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BBa_I13664
type: ComponentDefinition
persistent identity: BBa I13664
display id: BBa_I13664
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Cascaded Lac (medium) and Tet (medium) QPIs with constitutive promoter as input
creator: mit
created: 2004-07-19T11:00:00Z
modified: 2015-08-31T04:07:36Z
discontinued: false
dominant: false
experience: experience/None
group u list: _6_
m user id: 0
owner id: 101
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Measurement
component: BBa_Q01400
component: BBa_R0051
component: BBa_Q01121
sequence annotation: BBa_R0051
sequence annotation: BBa_Q01400
sequence annotation: BBa_Q01121
sequence: https://synbiohub.org/public/igem/BBa_I13664_sequence/1

BBa_Q01400
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display id: component2231502
version: 1
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access: public
definition: https://synbiohub.org/public/igem/BBa_Q01400/1

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display id: component2231462
version: 1
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access: public
definition: https://synbiohub.org/public/igem/BBa_R0051/1

BBa_Q01121
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display id: component2231484
version: 1
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access: public
definition: https://synbiohub.org/public/igem/BBa_Q01121/1

BBa_R0051
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component: BBa_R0051

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BBa_I6000
type: ComponentDefinition
persistent identity: BBa I6000
display id: BBa_I6000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: QPI Curve Test I0500.E0422.I0500.E0432
creator: Caitlin Conboy, Jennifer Braff
created: 2003-12-05T12:00:00Z
modified: 2015-08-31T04:07:42Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: QPI characterization control construct
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Measurement
component: BBa_I0500
component: BBa_E0022
component: BBa_B0012
component: BBa_B0034
component: BBa_B0010
component: BBa_E0032
sequence annotation: BBa_B0034
sequence annotation: BBa_B0010
sequence annotation: BBa_E0022
sequence annotation: BBa_I0500
sequence annotation: BBa_B0012
sequence annotation: BBa_E0032
sequence: https://synbiohub.org/public/igem/BBa_I6000_sequence/1

BBa_I0500
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display id: component968584
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access: public
definition: https://synbiohub.org/public/igem/BBa_I0500/1

BBa_E0022
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access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

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display id: component968549
version: 1
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access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

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display id: component968609
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access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_E0032
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display id: component968601
version: 1
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access: public
definition: https://synbiohub.org/public/igem/BBa_E0032/1

BBa_B0034
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access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

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access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0010
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display id: component968569
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access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_I0500
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display id: component968544
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access: public
definition: https://synbiohub.org/public/igem/BBa_I0500/1

BBa_B0034
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type: SequenceAnnotation
persistent identity: annotation968609
display id: annotation968609
version: 1
top level: BBa_I6000
location: https://synbiohub.org/public/igem/BBa_I6000/annotation968609/range968609/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I6000/annotation968609/range968609/1
type: Range
persistent identity: range968609
display id: range968609
version: 1
direction: direction/forward
top level: BBa_I6000
start: 4150
end: 4229
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation968549
display id: annotation968549
version: 1
top level: BBa_I6000
location: https://synbiohub.org/public/igem/BBa_I6000/annotation968549/range968549/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I6000/annotation968549/range968549/1
type: Range
persistent identity: range968549
display id: range968549
version: 1
direction: direction/forward
top level: BBa_I6000
start: 1219
end: 1230
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation968569
display id: annotation968569
version: 1
top level: BBa_I6000
location: https://synbiohub.org/public/igem/BBa_I6000/annotation968569/range968569/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I6000/annotation968569/range968569/1
type: Range
persistent identity: range968569
display id: range968569
version: 1
direction: direction/forward
top level: BBa_I6000
start: 2007
end: 2086
orientation: inline

BBa_E0022
type: SequenceAnnotation
persistent identity: annotation968561
display id: annotation968561
version: 1
top level: BBa_I6000
location: https://synbiohub.org/public/igem/BBa_I6000/annotation968561/range968561/1
component: BBa_E0022

https://synbiohub.org/public/igem/BBa_I6000/annotation968561/range968561/1
type: Range
persistent identity: range968561
display id: range968561
version: 1
direction: direction/forward
top level: BBa_I6000
start: 1237
end: 1998
orientation: inline

BBa_I0500
type: SequenceAnnotation
persistent identity: annotation968544
display id: annotation968544
version: 1
top level: BBa_I6000
location: https://synbiohub.org/public/igem/BBa_I6000/annotation968544/range968544/1
component: BBa_I0500

https://synbiohub.org/public/igem/BBa_I6000/annotation968544/range968544/1
type: Range
persistent identity: range968544
display id: range968544
version: 1
direction: direction/forward
top level: BBa_I6000
start: 1
end: 1210
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation968579
display id: annotation968579
version: 1
top level: BBa_I6000
location: https://synbiohub.org/public/igem/BBa_I6000/annotation968579/range968579/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I6000/annotation968579/range968579/1
type: Range
persistent identity: range968579
display id: range968579
version: 1
direction: direction/forward
top level: BBa_I6000
start: 2095
end: 2135
orientation: inline

BBa_E0032
type: SequenceAnnotation
persistent identity: annotation968601
display id: annotation968601
version: 1
top level: BBa_I6000
location: https://synbiohub.org/public/igem/BBa_I6000/annotation968601/range968601/1
component: BBa_E0032

https://synbiohub.org/public/igem/BBa_I6000/annotation968601/range968601/1
type: Range
persistent identity: range968601
display id: range968601
version: 1
direction: direction/forward
top level: BBa_I6000
start: 3380
end: 4141
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation968619
display id: annotation968619
version: 1
top level: BBa_I6000
location: https://synbiohub.org/public/igem/BBa_I6000/annotation968619/range968619/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I6000/annotation968619/range968619/1
type: Range
persistent identity: range968619
display id: range968619
version: 1
direction: direction/forward
top level: BBa_I6000
start: 4238
end: 4278
orientation: inline

BBa_I0500
type: SequenceAnnotation
persistent identity: annotation968584
display id: annotation968584
version: 1
top level: BBa_I6000
location: https://synbiohub.org/public/igem/BBa_I6000/annotation968584/range968584/1
component: BBa_I0500

https://synbiohub.org/public/igem/BBa_I6000/annotation968584/range968584/1
type: Range
persistent identity: range968584
display id: range968584
version: 1
direction: direction/forward
top level: BBa_I6000
start: 2144
end: 3353
orientation: inline

BBa_K389011
type: ComponentDefinition
persistent identity: BBa K389011
display id: BBa_K389011
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: VirA screening device
creator: Jonas Aretz
created: 2010-08-09T11:00:00Z
modified: 2015-05-08T01:12:19Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _517_
m user id: 0
owner id: 6670
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This part contains: - a mutated ''virG'' response regulator from the virA/G receptor system from ''Agrobacterium tumefaciens'' which works in ''Escherichia coli'' without an ''rpoA'' gene from ''A. tumefaciens'' - a kanamycin resistance under the control of a ''vir'' promotor This device is used to screen ''virA'' receptor mutants. The ''virA'' receptor is mutated in the BBa_K389010 BioBrick. The better the receptor detects a substance, the more ''virG'' is phosphorylated and the stronger the kanamycin resistance from this BioBrick is expressed. The BioBricks BBa_K389010 and BBa_K389011 have to be used together in one cell.
mutable notes: medium strong constitutive promotor, terminator to keep basal expression of kanamycin resistance low
mutable provenance: synthetic ''virG'' gene, ''vir'' promotor from ''A. tumefaciens'' C58, partsregistry
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K389003
component: BBa_K389002
component: BBa_J23110
component: BBa_B0010
component: BBa_K389005
sequence annotation: BBa_B0010
sequence annotation: BBa_J23110
sequence annotation: BBa_K389005
sequence annotation: BBa_K389002
sequence annotation: BBa_K389003
sequence: https://synbiohub.org/public/igem/BBa_K389011_sequence/1

BBa_K389003
type: Component
persistent identity: component2078379
display id: component2078379
version: 1
top level: BBa_K389011
access: public
definition: https://synbiohub.org/public/igem/BBa_K389003/1

BBa_K389002
type: Component
persistent identity: component2078370
display id: component2078370
version: 1
top level: BBa_K389011
access: public
definition: https://synbiohub.org/public/igem/BBa_K389002/1

BBa_J23110
type: Component
persistent identity: component2078366
display id: component2078366
version: 1
top level: BBa_K389011
access: public
definition: https://synbiohub.org/public/igem/BBa_J23110/1

BBa_B0010
type: Component
persistent identity: component2078371
display id: component2078371
version: 1
top level: BBa_K389011
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0010
type: Component
persistent identity: component2078373
display id: component2078373
version: 1
top level: BBa_K389011
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_K389005
type: Component
persistent identity: component2078382
display id: component2078382
version: 1
top level: BBa_K389011
access: public
definition: https://synbiohub.org/public/igem/BBa_K389005/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2078371
display id: annotation2078371
version: 1
top level: BBa_K389011
location: https://synbiohub.org/public/igem/BBa_K389011/annotation2078371/range2078371/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K389011/annotation2078371/range2078371/1
type: Range
persistent identity: range2078371
display id: range2078371
version: 1
direction: direction/forward
top level: BBa_K389011
start: 792
end: 871
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2078373
display id: annotation2078373
version: 1
top level: BBa_K389011
location: https://synbiohub.org/public/igem/BBa_K389011/annotation2078373/range2078373/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K389011/annotation2078373/range2078373/1
type: Range
persistent identity: range2078373
display id: range2078373
version: 1
direction: direction/forward
top level: BBa_K389011
start: 880
end: 959
orientation: inline

BBa_J23110
type: SequenceAnnotation
persistent identity: annotation2078366
display id: annotation2078366
version: 1
top level: BBa_K389011
location: https://synbiohub.org/public/igem/BBa_K389011/annotation2078366/range2078366/1
component: BBa_J23110

https://synbiohub.org/public/igem/BBa_K389011/annotation2078366/range2078366/1
type: Range
persistent identity: range2078366
display id: range2078366
version: 1
direction: direction/reverse
top level: BBa_K389011
start: 1
end: 35
orientation: inline

BBa_K389005
type: SequenceAnnotation
persistent identity: annotation2078382
display id: annotation2078382
version: 1
top level: BBa_K389011
location: https://synbiohub.org/public/igem/BBa_K389011/annotation2078382/range2078382/1
component: BBa_K389005

https://synbiohub.org/public/igem/BBa_K389011/annotation2078382/range2078382/1
type: Range
persistent identity: range2078382
display id: range2078382
version: 1
direction: direction/reverse
top level: BBa_K389011
start: 1132
end: 1950
orientation: inline

BBa_K389002
type: SequenceAnnotation
persistent identity: annotation2078370
display id: annotation2078370
version: 1
top level: BBa_K389011
location: https://synbiohub.org/public/igem/BBa_K389011/annotation2078370/range2078370/1
component: BBa_K389002

https://synbiohub.org/public/igem/BBa_K389011/annotation2078370/range2078370/1
type: Range
persistent identity: range2078370
display id: range2078370
version: 1
direction: direction/reverse
top level: BBa_K389011
start: 44
end: 783
orientation: inline

BBa_K389003
type: SequenceAnnotation
persistent identity: annotation2078379
display id: annotation2078379
version: 1
top level: BBa_K389011
location: https://synbiohub.org/public/igem/BBa_K389011/annotation2078379/range2078379/1
component: BBa_K389003

https://synbiohub.org/public/igem/BBa_K389011/annotation2078379/range2078379/1
type: Range
persistent identity: range2078379
display id: range2078379
version: 1
direction: direction/reverse
top level: BBa_K389011
start: 968
end: 1125
orientation: inline

InBTst
type: ComponentDefinition
persistent identity: BBa J05504
display id: BBa_J05504
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Sensing Device B (cI+LVA) TESTER (Pr+GFP+Prm+RFP)
creator: ETH Zurich iGEM Team 2005
created: 2005-09-21T11:00:00Z
modified: 2015-08-31T04:08:15Z
discontinued: false
dominant: false
experience: experience/None
group u list: _34_
m user id: 0
owner id: 398
owning group id: 34
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: A debug version of the event sensing and processing system for the iGEM2005 ETH "Counter" project: its purpose is to regulate cI+LVA (degradation tag) production dependent on an external event. It works somehow like an inverter: STATE 1: when IPTG is absent, the constitutively produced lacI protein will block the lacI+pL promoter so that no cI is produced. Thus downstream the Pr promoter will be constitutively active, producing GFP (green fluorescence), while Prm+ will have low or no activity (no/low RFP - red fluorescence). STATE 2: when IPTG is present, it will remove the lacI protein from the lacI+pL promoter and cI is produced. Thus downstream the Pr promoter will be repressed (no GFP, i.e. no green fluorescence) by cI, while Prm+ will be activated, producing RFP (red fluorescence).
mutable notes: For more details see Sensing Device and Event Processing Device documentation.
mutable provenance: Registry 7.05
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Measurement
component: BBa_C0051
component: BBa_B0010
component: BBa_E0040
component: BBa_R0051
component: BBa_B0034
component: BBa_R0011
component: BBa_B0012
component: BBa_E1010
component: BBa_R0040
component: BBa_I12007
component: BBa_C0012
component: BBa_B0011
sequence annotation: BBa_B0011
sequence annotation: BBa_R0040
sequence annotation: BBa_B0012
sequence annotation: BBa_B0034
sequence annotation: BBa_B0010
sequence annotation: BBa_E1010
sequence annotation: BBa_E0040
sequence annotation: BBa_R0011
sequence annotation: BBa_R0051
sequence annotation: BBa_C0051
sequence annotation: BBa_I12007
sequence annotation: BBa_C0012
sequence: https://synbiohub.org/public/igem/BBa_J05504_sequence/1

BBa_C0051
type: Component
persistent identity: component1702674
display id: component1702674
version: 1
top level: InBTst
access: public
definition: https://synbiohub.org/public/igem/BBa_C0051/1

BBa_B0010
type: Component
persistent identity: component1702631
display id: component1702631
version: 1
top level: InBTst
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_E0040
type: Component
persistent identity: component1702715
display id: component1702715
version: 1
top level: InBTst
access: public
definition: https://synbiohub.org/public/igem/BBa_E0040/1

BBa_R0051
type: Component
persistent identity: component1702705
display id: component1702705
version: 1
top level: InBTst
access: public
definition: https://synbiohub.org/public/igem/BBa_R0051/1

BBa_B0034
type: Component
persistent identity: component1702664
display id: component1702664
version: 1
top level: InBTst
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0034
type: Component
persistent identity: component1702755
display id: component1702755
version: 1
top level: InBTst
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_R0011
type: Component
persistent identity: component1702655
display id: component1702655
version: 1
top level: InBTst
access: public
definition: https://synbiohub.org/public/igem/BBa_R0011/1

BBa_B0012
type: Component
persistent identity: component1702641
display id: component1702641
version: 1
top level: InBTst
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0012
type: Component
persistent identity: component1702778
display id: component1702778
version: 1
top level: InBTst
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_E1010
type: Component
persistent identity: component1702762
display id: component1702762
version: 1
top level: InBTst
access: public
definition: https://synbiohub.org/public/igem/BBa_E1010/1

BBa_B0010
type: Component
persistent identity: component1702720
display id: component1702720
version: 1
top level: InBTst
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_R0040
type: Component
persistent identity: component1702606
display id: component1702606
version: 1
top level: InBTst
access: public
definition: https://synbiohub.org/public/igem/BBa_R0040/1

BBa_B0010
type: Component
persistent identity: component1702768
display id: component1702768
version: 1
top level: InBTst
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0012
type: Component
persistent identity: component1702684
display id: component1702684
version: 1
top level: InBTst
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_I12007
type: Component
persistent identity: component1702750
display id: component1702750
version: 1
top level: InBTst
access: public
definition: https://synbiohub.org/public/igem/BBa_I12007/1

BBa_B0034
type: Component
persistent identity: component1702614
display id: component1702614
version: 1
top level: InBTst
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_C0012
type: Component
persistent identity: component1702624
display id: component1702624
version: 1
top level: InBTst
access: public
definition: https://synbiohub.org/public/igem/BBa_C0012/1

BBa_B0012
type: Component
persistent identity: component1702730
display id: component1702730
version: 1
top level: InBTst
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0034
type: Component
persistent identity: component1702713
display id: component1702713
version: 1
top level: InBTst
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0011
type: Component
persistent identity: component1702692
display id: component1702692
version: 1
top level: InBTst
access: public
definition: https://synbiohub.org/public/igem/BBa_B0011/1

BBa_B0011
type: SequenceAnnotation
persistent identity: annotation1702692
display id: annotation1702692
version: 1
top level: InBTst
location: https://synbiohub.org/public/igem/BBa_J05504/annotation1702692/range1702692/1
component: BBa_B0011

https://synbiohub.org/public/igem/BBa_J05504/annotation1702692/range1702692/1
type: Range
persistent identity: range1702692
display id: range1702692
version: 1
direction: direction/forward
top level: InBTst
start: 2292
end: 2337
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1702606
display id: annotation1702606
version: 1
top level: InBTst
location: https://synbiohub.org/public/igem/BBa_J05504/annotation1702606/range1702606/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_J05504/annotation1702606/range1702606/1
type: Range
persistent identity: range1702606
display id: range1702606
version: 1
direction: direction/forward
top level: InBTst
start: 1
end: 54
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1702778
display id: annotation1702778
version: 1
top level: InBTst
location: https://synbiohub.org/public/igem/BBa_J05504/annotation1702778/range1702778/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_J05504/annotation1702778/range1702778/1
type: Range
persistent identity: range1702778
display id: range1702778
version: 1
direction: direction/forward
top level: InBTst
start: 4196
end: 4236
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1702713
display id: annotation1702713
version: 1
top level: InBTst
location: https://synbiohub.org/public/igem/BBa_J05504/annotation1702713/range1702713/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_J05504/annotation1702713/range1702713/1
type: Range
persistent identity: range1702713
display id: range1702713
version: 1
direction: direction/forward
top level: InBTst
start: 2403
end: 2414
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1702684
display id: annotation1702684
version: 1
top level: InBTst
location: https://synbiohub.org/public/igem/BBa_J05504/annotation1702684/range1702684/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_J05504/annotation1702684/range1702684/1
type: Range
persistent identity: range1702684
display id: range1702684
version: 1
direction: direction/forward
top level: InBTst
start: 2243
end: 2283
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1702768
display id: annotation1702768
version: 1
top level: InBTst
location: https://synbiohub.org/public/igem/BBa_J05504/annotation1702768/range1702768/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_J05504/annotation1702768/range1702768/1
type: Range
persistent identity: range1702768
display id: range1702768
version: 1
direction: direction/forward
top level: InBTst
start: 4108
end: 4187
orientation: inline

BBa_E1010
type: SequenceAnnotation
persistent identity: annotation1702762
display id: annotation1702762
version: 1
top level: InBTst
location: https://synbiohub.org/public/igem/BBa_J05504/annotation1702762/range1702762/1
component: BBa_E1010

https://synbiohub.org/public/igem/BBa_J05504/annotation1702762/range1702762/1
type: Range
persistent identity: range1702762
display id: range1702762
version: 1
direction: direction/forward
top level: InBTst
start: 3394
end: 4074
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation1702715
display id: annotation1702715
version: 1
top level: InBTst
location: https://synbiohub.org/public/igem/BBa_J05504/annotation1702715/range1702715/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_J05504/annotation1702715/range1702715/1
type: Range
persistent identity: range1702715
display id: range1702715
version: 1
direction: direction/forward
top level: InBTst
start: 2421
end: 3140
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1702664
display id: annotation1702664
version: 1
top level: InBTst
location: https://synbiohub.org/public/igem/BBa_J05504/annotation1702664/range1702664/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_J05504/annotation1702664/range1702664/1
type: Range
persistent identity: range1702664
display id: range1702664
version: 1
direction: direction/forward
top level: InBTst
start: 1442
end: 1453
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1702641
display id: annotation1702641
version: 1
top level: InBTst
location: https://synbiohub.org/public/igem/BBa_J05504/annotation1702641/range1702641/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_J05504/annotation1702641/range1702641/1
type: Range
persistent identity: range1702641
display id: range1702641
version: 1
direction: direction/forward
top level: InBTst
start: 1330
end: 1370
orientation: inline

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation1702655
display id: annotation1702655
version: 1
top level: InBTst
location: https://synbiohub.org/public/igem/BBa_J05504/annotation1702655/range1702655/1
component: BBa_R0011

https://synbiohub.org/public/igem/BBa_J05504/annotation1702655/range1702655/1
type: Range
persistent identity: range1702655
display id: range1702655
version: 1
direction: direction/forward
top level: InBTst
start: 1379
end: 1432
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1702720
display id: annotation1702720
version: 1
top level: InBTst
location: https://synbiohub.org/public/igem/BBa_J05504/annotation1702720/range1702720/1
component: BBa_B0010

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BBa_I13653
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persistent identity: BBa I13653
display id: BBa_I13653
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Cascaded Tet (medium) and Lac (weak) QPIs with constitutive promoter as input
creator: mit
created: 2004-07-19T11:00:00Z
modified: 2015-08-31T04:07:36Z
discontinued: false
dominant: false
experience: experience/None
group u list: _6_
m user id: 0
owner id: 101
owning group id: 7
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Measurement
component: BBa_Q03121
component: BBa_R0051
component: BBa_Q01400
sequence annotation: BBa_R0051
sequence annotation: BBa_Q03121
sequence annotation: BBa_Q01400
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BBa_I7025
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was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: R0010.E0021.B0025.E0031
creator: Caitlin Conboy
created: 2004-04-22T11:00:00Z
modified: 2015-08-31T04:07:45Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Terminator Characterization Construct
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owned by: myers
star: false
type: DnaRegion
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component: BBa_E0021
component: BBa_E0031
component: BBa_R0010
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display id: BBa_I13008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS Test R0011.B0031.E0130
creator: jasonk
created: 2004-06-30T11:00:00Z
modified: 2015-08-31T04:07:32Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_6_
m user id: 0
owner id: 135
owning group id: 7
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Parts I13007-I130014 are RBS test constructs for RBS characterization. Each consists of Lac or Tet promoter, one of the four RBS's B0030-B0033, a YFP coding protein, and B0015 terminator.
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owned by: myers
star: false
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type: ComponentDefinition
persistent identity: BBa I6322
display id: BBa_I6322
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: QPI Curve Test I0500.E0420.I0500.Q04530.E0430
creator: cconboy
created: 2004-05-09T11:00:00Z
modified: 2015-08-31T04:07:44Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_1_
m user id: 0
owner id: 60
owning group id: 7
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000804
role: partType/Measurement
component: BBa_B0010
component: BBa_B0034
component: BBa_E0020
component: BBa_B0012
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component: BBa_R0053
component: BBa_E0030
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sequence annotation: BBa_B0012
sequence annotation: BBa_I0500
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BBa_B0010
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version: 1
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BBa_B0034
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BBa_B0034
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BBa_B0010
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BBa_B0010
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BBa_R0053
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display id: annotation980641
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BBa_B0012
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BBa_I0500
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BBa_E0030
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BBa_B0010
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BBa_B0034
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BBa_B0012
type: SequenceAnnotation
persistent identity: annotation980668
display id: annotation980668
version: 1
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display id: range980668
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BBa_I13624
type: ComponentDefinition
persistent identity: BBa I13624
display id: BBa_I13624
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Cascaded Lac (medium) and Tet (medium) QPIs
creator: mit
created: 2004-07-19T11:00:00Z
modified: 2015-08-31T04:07:36Z
discontinued: false
dominant: false
experience: experience/None
group u list: _6_
m user id: 0
owner id: 101
owning group id: 7
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Measurement
component: BBa_Q01400
component: BBa_Q01121
sequence annotation: BBa_Q01400
sequence annotation: BBa_Q01121
sequence: https://synbiohub.org/public/igem/BBa_I13624_sequence/1

BBa_Q01400
type: Component
persistent identity: component2228995
display id: component2228995
version: 1
top level: BBa_I13624
access: public
definition: https://synbiohub.org/public/igem/BBa_Q01400/1

BBa_Q01121
type: Component
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display id: component2228977
version: 1
top level: BBa_I13624
access: public
definition: https://synbiohub.org/public/igem/BBa_Q01121/1

BBa_Q01400
type: SequenceAnnotation
persistent identity: annotation2228995
display id: annotation2228995
version: 1
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location: https://synbiohub.org/public/igem/BBa_I13624/annotation2228995/range2228995/1
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BBa_Q01121
type: SequenceAnnotation
persistent identity: annotation2228977
display id: annotation2228977
version: 1
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location: https://synbiohub.org/public/igem/BBa_I13624/annotation2228977/range2228977/1
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persistent identity: range2228977
display id: range2228977
version: 1
direction: direction/reverse
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BBa_I7021
type: ComponentDefinition
persistent identity: BBa I7021
display id: BBa_I7021
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: R0010.E0021.B0021.E0031
creator: Caitlin Conboy
created: 2004-04-22T11:00:00Z
modified: 2015-08-31T04:07:45Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Terminator Characterization Construct
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Measurement
component: BBa_R0010
component: BBa_B0021
component: BBa_E0021
component: BBa_E0031
sequence annotation: BBa_E0021
sequence annotation: BBa_R0010
sequence annotation: BBa_E0031
sequence annotation: BBa_B0021
sequence: https://synbiohub.org/public/igem/BBa_I7021_sequence/1

BBa_R0010
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persistent identity: component944058
display id: component944058
version: 1
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access: public
definition: https://synbiohub.org/public/igem/BBa_R0010/1

BBa_B0021
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persistent identity: component944077
display id: component944077
version: 1
top level: BBa_I7021
access: public
definition: https://synbiohub.org/public/igem/BBa_B0021/1

BBa_E0021
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persistent identity: component944074
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version: 1
top level: BBa_I7021
access: public
definition: https://synbiohub.org/public/igem/BBa_E0021/1

BBa_E0031
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persistent identity: component944088
display id: component944088
version: 1
top level: BBa_I7021
access: public
definition: https://synbiohub.org/public/igem/BBa_E0031/1

BBa_E0021
type: SequenceAnnotation
persistent identity: annotation944074
display id: annotation944074
version: 1
top level: BBa_I7021
location: https://synbiohub.org/public/igem/BBa_I7021/annotation944074/range944074/1
component: BBa_E0021

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type: SequenceAnnotation
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display id: annotation944058
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display id: range944058
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type: SequenceAnnotation
persistent identity: annotation944088
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version: 1
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location: https://synbiohub.org/public/igem/BBa_I7021/annotation944088/range944088/1
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BBa_B0021
type: SequenceAnnotation
persistent identity: annotation944077
display id: annotation944077
version: 1
top level: BBa_I7021
location: https://synbiohub.org/public/igem/BBa_I7021/annotation944077/range944077/1
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persistent identity: range944077
display id: range944077
version: 1
direction: direction/forward
top level: BBa_I7021
start: 957
end: 1002
orientation: inline

BBa_S03367
type: ComponentDefinition
persistent identity: BBa S03367
display id: BBa_S03367
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Input to switch (-LacI, +GFP)
creator: Lane Weaver
created: 2005-10-31T12:00:00Z
modified: 2015-05-08T01:14:22Z
discontinued: false
dominant: false
group u list: _40_
m user id: 0
owner id: 335
owning group id: 40
sample status: Not in stock
status: status/Unavailable
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Measurement
sequence: https://synbiohub.org/public/igem/BBa_S03367_sequence/1

BBa_I13014
type: ComponentDefinition
persistent identity: BBa I13014
display id: BBa_I13014
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS Test R0040.B0033.E0130
creator: jasonk
created: 2004-06-30T11:00:00Z
modified: 2015-08-31T04:07:32Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_6_
m user id: 0
owner id: 135
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Parts I13007-I130014 are RBS test constructs for RBS characterization. Each consists of Lac or Tet promoter, one of the four RBS's B0030-B0033, a YFP coding protein, and B0015 terminator.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Measurement
component: BBa_B0012
component: BBa_E0030
component: BBa_B0033
component: BBa_B0010
component: BBa_R0040
sequence annotation: BBa_B0033
sequence annotation: BBa_R0040
sequence annotation: BBa_E0030
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_I13014_sequence/1

BBa_B0012
type: Component
persistent identity: component946910
display id: component946910
version: 1
top level: BBa_I13014
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_E0030
type: Component
persistent identity: component946895
display id: component946895
version: 1
top level: BBa_I13014
access: public
definition: https://synbiohub.org/public/igem/BBa_E0030/1

BBa_B0033
type: Component
persistent identity: component946892
display id: component946892
version: 1
top level: BBa_I13014
access: public
definition: https://synbiohub.org/public/igem/BBa_B0033/1

BBa_B0010
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persistent identity: component946900
display id: component946900
version: 1
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access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_R0040
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persistent identity: component946881
display id: component946881
version: 1
top level: BBa_I13014
access: public
definition: https://synbiohub.org/public/igem/BBa_R0040/1

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type: SequenceAnnotation
persistent identity: annotation946892
display id: annotation946892
version: 1
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location: https://synbiohub.org/public/igem/BBa_I13014/annotation946892/range946892/1
component: BBa_B0033

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persistent identity: range946892
display id: range946892
version: 1
direction: direction/forward
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orientation: inline

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display id: annotation946881
version: 1
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persistent identity: range946881
display id: range946881
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BBa_E0030
type: SequenceAnnotation
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display id: annotation946895
version: 1
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component: BBa_E0030

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persistent identity: range946895
display id: range946895
version: 1
direction: direction/forward
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end: 802
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation946900
display id: annotation946900
version: 1
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component: BBa_B0010

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persistent identity: range946900
display id: range946900
version: 1
direction: direction/forward
top level: BBa_I13014
start: 811
end: 890
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation946910
display id: annotation946910
version: 1
top level: BBa_I13014
location: https://synbiohub.org/public/igem/BBa_I13014/annotation946910/range946910/1
component: BBa_B0012

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type: Range
persistent identity: range946910
display id: range946910
version: 1
direction: direction/forward
top level: BBa_I13014
start: 899
end: 939
orientation: inline

BBa_I6304
type: ComponentDefinition
persistent identity: BBa I6304
display id: BBa_I6304
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: QPI Curve Test I0500.E0420.I0500.Q04122.E0430
creator: cconboy
created: 2004-05-09T11:00:00Z
modified: 2015-08-31T04:07:44Z
discontinued: true
dominant: false
experience: experience/None
group u list: _11_1_
m user id: 0
owner id: 60
owning group id: 7
sample status: Discontinued
status: status/Deleted
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Measurement
component: BBa_B0010
component: BBa_B0034
component: BBa_E0020
component: BBa_C0012
component: BBa_R0010
component: BBa_I0500
component: BBa_E0030
component: BBa_B0012
sequence annotation: BBa_B0012
sequence annotation: BBa_B0010
sequence annotation: BBa_I0500
sequence annotation: BBa_B0034
sequence annotation: BBa_E0020
sequence annotation: BBa_C0012
sequence annotation: BBa_E0030
sequence annotation: BBa_R0010
sequence: https://synbiohub.org/public/igem/BBa_I6304_sequence/1

BBa_B0010
type: Component
persistent identity: component979927
display id: component979927
version: 1
top level: BBa_I6304
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0034
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persistent identity: component979947
display id: component979947
version: 1
top level: BBa_I6304
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_E0020
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persistent identity: component979922
display id: component979922
version: 1
top level: BBa_I6304
access: public
definition: https://synbiohub.org/public/igem/BBa_E0020/1

BBa_C0012
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display id: component979957
version: 1
top level: BBa_I6304
access: public
definition: https://synbiohub.org/public/igem/BBa_C0012/1

BBa_B0034
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display id: component979920
version: 1
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access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_R0010
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display id: component979990
version: 1
top level: BBa_I6304
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definition: https://synbiohub.org/public/igem/BBa_R0010/1

BBa_I0500
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display id: component979915
version: 1
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access: public
definition: https://synbiohub.org/public/igem/BBa_I0500/1

BBa_B0034
type: Component
persistent identity: component980000
display id: component980000
version: 1
top level: BBa_I6304
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_I0500
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persistent identity: component979942
display id: component979942
version: 1
top level: BBa_I6304
access: public
definition: https://synbiohub.org/public/igem/BBa_I0500/1

BBa_B0010
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persistent identity: component979964
display id: component979964
version: 1
top level: BBa_I6304
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_E0030
type: Component
persistent identity: component980002
display id: component980002
version: 1
top level: BBa_I6304
access: public
definition: https://synbiohub.org/public/igem/BBa_E0030/1

BBa_B0012
type: Component
persistent identity: component980017
display id: component980017
version: 1
top level: BBa_I6304
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0012
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persistent identity: component979937
display id: component979937
version: 1
top level: BBa_I6304
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0012
type: Component
persistent identity: component979974
display id: component979974
version: 1
top level: BBa_I6304
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0010
type: Component
persistent identity: component980007
display id: component980007
version: 1
top level: BBa_I6304
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation979937
display id: annotation979937
version: 1
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location: https://synbiohub.org/public/igem/BBa_I6304/annotation979937/range979937/1
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display id: range979937
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orientation: inline

BBa_B0012
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BBa_B0010
type: SequenceAnnotation
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top level: BBa_I6304
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persistent identity: range979964
display id: range979964
version: 1
direction: direction/forward
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end: 4581
orientation: inline

BBa_I0500
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display id: annotation979942
version: 1
top level: BBa_I6304
location: https://synbiohub.org/public/igem/BBa_I6304/annotation979942/range979942/1
component: BBa_I0500

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persistent identity: range979942
display id: range979942
version: 1
direction: direction/forward
top level: BBa_I6304
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orientation: inline

BBa_B0034
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display id: annotation980000
version: 1
top level: BBa_I6304
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description: TetR repressible GFP generator
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type: Component
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display id: component1250309
version: 1
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access: public
definition: https://synbiohub.org/public/igem/BBa_E0040/1

BBa_B0010
type: Component
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display id: component1250314
version: 1
top level: BBa_I7100
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0010
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display id: annotation1250314
version: 1
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location: https://synbiohub.org/public/igem/BBa_I7100/annotation1250314/range1250314/1
component: BBa_B0010

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BBa_B0012
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version: 1
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component: BBa_B0012

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display id: range1250324
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direction: direction/forward
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start: 900
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BBa_B0030
type: SequenceAnnotation
persistent identity: annotation1250306
display id: annotation1250306
version: 1
top level: BBa_I7100
location: https://synbiohub.org/public/igem/BBa_I7100/annotation1250306/range1250306/1
component: BBa_B0030

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BBa_E0040
type: SequenceAnnotation
persistent identity: annotation1250309
display id: annotation1250309
version: 1
top level: BBa_I7100
location: https://synbiohub.org/public/igem/BBa_I7100/annotation1250309/range1250309/1
component: BBa_E0040

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display id: range1250309
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BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1250295
display id: annotation1250295
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location: https://synbiohub.org/public/igem/BBa_I7100/annotation1250295/range1250295/1
component: BBa_R0040

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persistent identity: range1250295
display id: range1250295
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start: 1
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BBa_I13533
type: ComponentDefinition
persistent identity: BBa I13533
display id: BBa_I13533
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Screening Plasmid 2.5
creator: Josh Michener
created: 2005-07-28T11:00:00Z
modified: 2015-08-31T04:07:35Z
discontinued: false
dominant: false
group u list: _11_
m user id: 0
owner id: 253
owning group id: 6
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Measurement
component: BBa_I13527
component: BBa_I13516
sequence annotation: BBa_I13527
sequence annotation: BBa_I13516
sequence: https://synbiohub.org/public/igem/BBa_I13533_sequence/1

BBa_I13527
type: Component
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display id: component2226177
version: 1
top level: BBa_I13533
access: public
definition: https://synbiohub.org/public/igem/BBa_I13527/1

BBa_I13516
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display id: component2226159
version: 1
top level: BBa_I13533
access: public
definition: https://synbiohub.org/public/igem/BBa_I13516/1

BBa_I13527
type: SequenceAnnotation
persistent identity: annotation2226177
display id: annotation2226177
version: 1
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component: BBa_I13527

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display id: range2226177
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BBa_I13516
type: SequenceAnnotation
persistent identity: annotation2226159
display id: annotation2226159
version: 1
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location: https://synbiohub.org/public/igem/BBa_I13533/annotation2226159/range2226159/1
component: BBa_I13516

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type: Range
persistent identity: range2226159
display id: range2226159
version: 1
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start: 1
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BBa_K389423
type: ComponentDefinition
persistent identity: BBa K389423
display id: BBa_K389423
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: VirA/G signaling system + sensitivity tuner + luciferase
creator: Jonas Aretz
created: 2010-10-10T11:00:00Z
modified: 2015-05-08T01:12:19Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _517_
m user id: 0
owner id: 6670
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part combines a complete VirA/G signaling system with a sensitivity tuner before a luciferase gene under the control of a ''vir'' promoter. The sensitivity tuner enhances a signal from a promoter so it enhances the readout luciferase.
mutable notes: sensitivity tuner to enhance the luciferase signal
mutable provenance: * luciferase from pGL4.10luc2 (Promega) * sensitivity tuner part, terminators and constitutive promoter from the partsregistry * ''virG'' was synthesized by Mr. Gene * ''virA'' and ''vir'' promoter from ''Agrobacterium tumefaciens'' C58, TI-plasmid
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K389017
component: BBa_K389403
sequence annotation: BBa_K389017
sequence annotation: BBa_K389403
sequence: https://synbiohub.org/public/igem/BBa_K389423_sequence/1

BBa_K389017
type: Component
persistent identity: component2313944
display id: component2313944
version: 1
top level: BBa_K389423
access: public
definition: https://synbiohub.org/public/igem/BBa_K389017/1

BBa_K389403
type: Component
persistent identity: component2313969
display id: component2313969
version: 1
top level: BBa_K389423
access: public
definition: https://synbiohub.org/public/igem/BBa_K389403/1

BBa_K389017
type: SequenceAnnotation
persistent identity: annotation2313944
display id: annotation2313944
version: 1
top level: BBa_K389423
location: https://synbiohub.org/public/igem/BBa_K389423/annotation2313944/range2313944/1
component: BBa_K389017

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display id: range2313944
version: 1
direction: direction/reverse
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end: 3699
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BBa_K389403
type: SequenceAnnotation
persistent identity: annotation2313969
display id: annotation2313969
version: 1
top level: BBa_K389423
location: https://synbiohub.org/public/igem/BBa_K389423/annotation2313969/range2313969/1
component: BBa_K389403

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type: Range
persistent identity: range2313969
display id: range2313969
version: 1
direction: direction/reverse
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start: 3708
end: 6621
orientation: inline

BBa_I6001
type: ComponentDefinition
persistent identity: BBa I6001
display id: BBa_I6001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: QPI Curve Test I0500.E0422.I0500.Q04121.E0432
creator: Caitlin Conboy, Jennifer Braff
created: 2003-12-05T12:00:00Z
modified: 2015-08-31T04:07:42Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: QPI characterization assembly
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000804
role: partType/Measurement
component: BBa_I0500
component: BBa_E0422
component: BBa_E0432
component: BBa_Q04121
sequence annotation: BBa_E0422
sequence annotation: BBa_Q04121
sequence annotation: BBa_E0432
sequence annotation: BBa_I0500
sequence: https://synbiohub.org/public/igem/BBa_I6001_sequence/1

BBa_I0500
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persistent identity: component2234547
display id: component2234547
version: 1
top level: BBa_I6001
access: public
definition: https://synbiohub.org/public/igem/BBa_I0500/1

BBa_E0422
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persistent identity: component2234561
display id: component2234561
version: 1
top level: BBa_I6001
access: public
definition: https://synbiohub.org/public/igem/BBa_E0422/1

BBa_E0432
type: Component
persistent identity: component2234598
display id: component2234598
version: 1
top level: BBa_I6001
access: public
definition: https://synbiohub.org/public/igem/BBa_E0432/1

BBa_I0500
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persistent identity: component2234565
display id: component2234565
version: 1
top level: BBa_I6001
access: public
definition: https://synbiohub.org/public/igem/BBa_I0500/1

BBa_Q04121
type: Component
persistent identity: component2234584
display id: component2234584
version: 1
top level: BBa_I6001
access: public
definition: https://synbiohub.org/public/igem/BBa_Q04121/1

BBa_E0422
type: SequenceAnnotation
persistent identity: annotation2234561
display id: annotation2234561
version: 1
top level: BBa_I6001
location: https://synbiohub.org/public/igem/BBa_I6001/annotation2234561/range2234561/1
component: BBa_E0422

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display id: range2234561
version: 1
direction: direction/reverse
top level: BBa_I6001
start: 1219
end: 2135
orientation: inline

BBa_Q04121
type: SequenceAnnotation
persistent identity: annotation2234584
display id: annotation2234584
version: 1
top level: BBa_I6001
location: https://synbiohub.org/public/igem/BBa_I6001/annotation2234584/range2234584/1
component: BBa_Q04121

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persistent identity: range2234584
display id: range2234584
version: 1
direction: direction/reverse
top level: BBa_I6001
start: 3362
end: 4732
orientation: inline

BBa_E0432
type: SequenceAnnotation
persistent identity: annotation2234598
display id: annotation2234598
version: 1
top level: BBa_I6001
location: https://synbiohub.org/public/igem/BBa_I6001/annotation2234598/range2234598/1
component: BBa_E0432

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persistent identity: range2234598
display id: range2234598
version: 1
direction: direction/reverse
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start: 4741
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BBa_I0500
type: SequenceAnnotation
persistent identity: annotation2234547
display id: annotation2234547
version: 1
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display id: range2234547
version: 1
direction: direction/reverse
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BBa_I0500
type: SequenceAnnotation
persistent identity: annotation2234565
display id: annotation2234565
version: 1
top level: BBa_I6001
location: https://synbiohub.org/public/igem/BBa_I6001/annotation2234565/range2234565/1
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type: Range
persistent identity: range2234565
display id: range2234565
version: 1
direction: direction/reverse
top level: BBa_I6001
start: 2144
end: 3353
orientation: inline

BBa_I6401
type: ComponentDefinition
persistent identity: BBa I6401
display id: BBa_I6401
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: YFP(AAV tagged) Reporter Device
creator: Alex Mallet
created: 2004-10-14T11:00:00Z
modified: 2015-08-31T04:07:44Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_6_
m user id: 0
owner id: 135
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: YFP tagged with an AAV ssrA tag with transcription initiated from pTet
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Measurement
component: BBa_B0010
component: BBa_R0040
component: BBa_B0034
component: BBa_E0034
component: BBa_B0012
sequence annotation: BBa_B0034
sequence annotation: BBa_E0034
sequence annotation: BBa_B0010
sequence annotation: BBa_R0040
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_I6401_sequence/1

BBa_B0010
type: Component
persistent identity: component1248721
display id: component1248721
version: 1
top level: BBa_I6401
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_R0040
type: Component
persistent identity: component1248700
display id: component1248700
version: 1
top level: BBa_I6401
access: public
definition: https://synbiohub.org/public/igem/BBa_R0040/1

BBa_B0034
type: Component
persistent identity: component1248708
display id: component1248708
version: 1
top level: BBa_I6401
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_E0034
type: Component
persistent identity: component1248716
display id: component1248716
version: 1
top level: BBa_I6401
access: public
definition: https://synbiohub.org/public/igem/BBa_E0034/1

BBa_B0012
type: Component
persistent identity: component1248731
display id: component1248731
version: 1
top level: BBa_I6401
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1248708
display id: annotation1248708
version: 1
top level: BBa_I6401
location: https://synbiohub.org/public/igem/BBa_I6401/annotation1248708/range1248708/1
component: BBa_B0034

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type: Range
persistent identity: range1248708
display id: range1248708
version: 1
direction: direction/forward
top level: BBa_I6401
start: 63
end: 74
orientation: inline

BBa_E0034
type: SequenceAnnotation
persistent identity: annotation1248716
display id: annotation1248716
version: 1
top level: BBa_I6401
location: https://synbiohub.org/public/igem/BBa_I6401/annotation1248716/range1248716/1
component: BBa_E0034

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type: Range
persistent identity: range1248716
display id: range1248716
version: 1
direction: direction/forward
top level: BBa_I6401
start: 81
end: 842
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1248721
display id: annotation1248721
version: 1
top level: BBa_I6401
location: https://synbiohub.org/public/igem/BBa_I6401/annotation1248721/range1248721/1
component: BBa_B0010

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type: Range
persistent identity: range1248721
display id: range1248721
version: 1
direction: direction/forward
top level: BBa_I6401
start: 851
end: 930
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1248700
display id: annotation1248700
version: 1
top level: BBa_I6401
location: https://synbiohub.org/public/igem/BBa_I6401/annotation1248700/range1248700/1
component: BBa_R0040

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type: Range
persistent identity: range1248700
display id: range1248700
version: 1
direction: direction/forward
top level: BBa_I6401
start: 1
end: 54
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1248731
display id: annotation1248731
version: 1
top level: BBa_I6401
location: https://synbiohub.org/public/igem/BBa_I6401/annotation1248731/range1248731/1
component: BBa_B0012

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type: Range
persistent identity: range1248731
display id: range1248731
version: 1
direction: direction/forward
top level: BBa_I6401
start: 939
end: 979
orientation: inline

BBa_I7014
type: ComponentDefinition
persistent identity: BBa I7014
display id: BBa_I7014
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: R0010.E0021.B0014.E0031
creator: Caitlin Conboy
created: 2004-04-22T11:00:00Z
modified: 2015-08-31T04:07:45Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Terminator Characterization Construct
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Measurement
component: BBa_E0021
component: BBa_B0012
component: BBa_R0010
component: BBa_E0031
component: BBa_B0011
sequence annotation: BBa_B0011
sequence annotation: BBa_R0010
sequence annotation: BBa_E0021
sequence annotation: BBa_B0012
sequence annotation: BBa_E0031
sequence: https://synbiohub.org/public/igem/BBa_I7014_sequence/1

BBa_E0021
type: Component
persistent identity: component951023
display id: component951023
version: 1
top level: BBa_I7014
access: public
definition: https://synbiohub.org/public/igem/BBa_E0021/1

BBa_B0012
type: Component
persistent identity: component951032
display id: component951032
version: 1
top level: BBa_I7014
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_R0010
type: Component
persistent identity: component951007
display id: component951007
version: 1
top level: BBa_I7014
access: public
definition: https://synbiohub.org/public/igem/BBa_R0010/1

BBa_E0031
type: Component
persistent identity: component951052
display id: component951052
version: 1
top level: BBa_I7014
access: public
definition: https://synbiohub.org/public/igem/BBa_E0031/1

BBa_B0011
type: Component
persistent identity: component951040
display id: component951040
version: 1
top level: BBa_I7014
access: public
definition: https://synbiohub.org/public/igem/BBa_B0011/1

BBa_B0011
type: SequenceAnnotation
persistent identity: annotation951040
display id: annotation951040
version: 1
top level: BBa_I7014
location: https://synbiohub.org/public/igem/BBa_I7014/annotation951040/range951040/1
component: BBa_B0011

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persistent identity: range951040
display id: range951040
version: 1
direction: direction/forward
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start: 1006
end: 1051
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation951007
display id: annotation951007
version: 1
top level: BBa_I7014
location: https://synbiohub.org/public/igem/BBa_I7014/annotation951007/range951007/1
component: BBa_R0010

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type: Range
persistent identity: range951007
display id: range951007
version: 1
direction: direction/forward
top level: BBa_I7014
start: 1
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orientation: inline

BBa_E0021
type: SequenceAnnotation
persistent identity: annotation951023
display id: annotation951023
version: 1
top level: BBa_I7014
location: https://synbiohub.org/public/igem/BBa_I7014/annotation951023/range951023/1
component: BBa_E0021

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display id: range951023
version: 1
direction: direction/forward
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BBa_B0012
type: SequenceAnnotation
persistent identity: annotation951032
display id: annotation951032
version: 1
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location: https://synbiohub.org/public/igem/BBa_I7014/annotation951032/range951032/1
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type: Range
persistent identity: range951032
display id: range951032
version: 1
direction: direction/forward
top level: BBa_I7014
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BBa_E0031
type: SequenceAnnotation
persistent identity: annotation951052
display id: annotation951052
version: 1
top level: BBa_I7014
location: https://synbiohub.org/public/igem/BBa_I7014/annotation951052/range951052/1
component: BBa_E0031

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type: Range
persistent identity: range951052
display id: range951052
version: 1
direction: direction/forward
top level: BBa_I7014
start: 1060
end: 1799
orientation: inline

BBa_I6010
type: ComponentDefinition
persistent identity: BBa I6010
display id: BBa_I6010
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: QPI Curve Test I0500.E0422.I0500.Q04500.E0432
creator: Caitlin Conboy, Jennifer Braff
created: 2003-12-05T12:00:00Z
modified: 2015-08-31T04:07:42Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: QPI characterization assembly
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Measurement
component: BBa_B0034
component: BBa_B0012
component: BBa_I0500
component: BBa_R0050
component: BBa_E0022
component: BBa_B0010
component: BBa_C0050
component: BBa_E0032
sequence annotation: BBa_C0050
sequence annotation: BBa_B0010
sequence annotation: BBa_I0500
sequence annotation: BBa_B0034
sequence annotation: BBa_R0050
sequence annotation: BBa_B0012
sequence annotation: BBa_E0022
sequence annotation: BBa_E0032
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BBa_B0012
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BBa_B0012
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BBa_E0032
type: SequenceAnnotation
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BBa_I7106
type: ComponentDefinition
persistent identity: BBa I7106
display id: BBa_I7106
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: R0011.E0840
creator: Jennifer Braff
created: 2005-07-27T11:00:00Z
modified: 2015-08-31T04:07:45Z
discontinued: false
dominant: false
group u list: _11_
m user id: 0
owner id: 61
owning group id: 1
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: R0011.E0840 (S_N; strong rbs; high PoPS)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Measurement
component: BBa_R0011
component: BBa_B0030
component: BBa_B0012
component: BBa_E0040
component: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_B0010
sequence annotation: BBa_B0030
sequence annotation: BBa_E0040
sequence annotation: BBa_R0011
sequence: https://synbiohub.org/public/igem/BBa_I7106_sequence/1

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BBa_B0012
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definition: https://synbiohub.org/public/igem/BBa_B0012/1

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type: Component
persistent identity: component1596591
display id: component1596591
version: 1
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definition: https://synbiohub.org/public/igem/BBa_E0040/1

BBa_B0010
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version: 1
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BBa_B0012
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BBa_B0010
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BBa_B0030
type: SequenceAnnotation
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BBa_R0011
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persistent identity: annotation1596576
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version: 1
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BBa_I13534
type: ComponentDefinition
persistent identity: BBa I13534
display id: BBa_I13534
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Screening Plasmid 2.6
creator: Josh Michener
created: 2005-07-28T11:00:00Z
modified: 2015-08-31T04:07:35Z
discontinued: false
dominant: true
group u list: _11_
m user id: 0
owner id: 253
owning group id: 6
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Measurement
component: BBa_I13506
component: BBa_I13528
sequence annotation: BBa_I13506
sequence annotation: BBa_I13528
sequence: https://synbiohub.org/public/igem/BBa_I13534_sequence/1

BBa_I13506
type: Component
persistent identity: component2269410
display id: component2269410
version: 1
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access: public
definition: https://synbiohub.org/public/igem/BBa_I13506/1

BBa_I13528
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persistent identity: component2269430
display id: component2269430
version: 1
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access: public
definition: https://synbiohub.org/public/igem/BBa_I13528/1

BBa_I13506
type: SequenceAnnotation
persistent identity: annotation2269410
display id: annotation2269410
version: 1
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version: 1
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BBa_K389422
type: ComponentDefinition
persistent identity: BBa K389422
display id: BBa_K389422
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: VirA/G signaling system + sensitivity tuner + luciferase
creator: Jonas Aretz
created: 2010-10-10T11:00:00Z
modified: 2015-05-08T01:12:19Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _517_
m user id: 0
owner id: 6670
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part combines a complete VirA/G signaling system with a sensitivity tuner before a luciferase gene under the control of a ''vir'' promoter. The sensitivity tuner enhances a signal from a promoter so it enhances the readout luciferase.
mutable notes: sensitivity tuner to enhance the luciferase signal
mutable provenance: * luciferase from pGL4.10luc2 (Promega) * sensitivity tuner part, terminators and constitutive promoter from the partsregistry * ''virG'' was synthesized by Mr. Gene * ''virA'' and ''vir'' promoter from ''Agrobacterium tumefaciens'' C58, TI-plasmid
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K389017
component: BBa_K389402
sequence annotation: BBa_K389402
sequence annotation: BBa_K389017
sequence: https://synbiohub.org/public/igem/BBa_K389422_sequence/1

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type: Component
persistent identity: component2313896
display id: component2313896
version: 1
top level: BBa_K389422
access: public
definition: https://synbiohub.org/public/igem/BBa_K389017/1

BBa_K389402
type: Component
persistent identity: component2313920
display id: component2313920
version: 1
top level: BBa_K389422
access: public
definition: https://synbiohub.org/public/igem/BBa_K389402/1

BBa_K389402
type: SequenceAnnotation
persistent identity: annotation2313920
display id: annotation2313920
version: 1
top level: BBa_K389422
location: https://synbiohub.org/public/igem/BBa_K389422/annotation2313920/range2313920/1
component: BBa_K389402

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display id: range2313920
version: 1
direction: direction/reverse
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type: SequenceAnnotation
persistent identity: annotation2313896
display id: annotation2313896
version: 1
top level: BBa_K389422
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component: BBa_K389017

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persistent identity: range2313896
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version: 1
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lacITst
type: ComponentDefinition
persistent identity: BBa J05505
display id: BBa_J05505
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Lambda-lacI input system TESTER (IPTG controlled lacI+pL efficiency)
creator: ETH Zurich iGEM Team 2005
created: 2005-09-21T11:00:00Z
modified: 2015-08-31T04:08:16Z
discontinued: false
dominant: true
experience: experience/None
group u list: _34_
m user id: 0
owner id: 398
owning group id: 34
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Constitutive (tetR) lacI-production (lacI+LVA) to block promoter (lacI+pL) in ground state of a 2-state system. lacI+pL can be activated, e.g. over IPTG, thus leading to the production of GFP (green fluorescence). This device allows to estimate repressed (lacI) activity/full activity under ITPG absence/presence.
mutable notes: For more details see Sensing Device and Event Processing Device documentation.
mutable provenance: Registry 7.05
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Measurement
component: BBa_B0012
component: BBa_R0040
component: BBa_R0011
component: BBa_E0040
component: BBa_B0010
component: BBa_B0034
component: BBa_C0012
sequence annotation: BBa_R0040
sequence annotation: BBa_B0010
sequence annotation: BBa_C0012
sequence annotation: BBa_R0011
sequence annotation: BBa_B0012
sequence annotation: BBa_B0034
sequence annotation: BBa_E0040
sequence: https://synbiohub.org/public/igem/BBa_J05505_sequence/1

BBa_B0012
type: Component
persistent identity: component1702873
display id: component1702873
version: 1
top level: lacITst
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_R0040
type: Component
persistent identity: component1702798
display id: component1702798
version: 1
top level: lacITst
access: public
definition: https://synbiohub.org/public/igem/BBa_R0040/1

BBa_R0011
type: Component
persistent identity: component1702847
display id: component1702847
version: 1
top level: lacITst
access: public
definition: https://synbiohub.org/public/igem/BBa_R0011/1

BBa_E0040
type: Component
persistent identity: component1702858
display id: component1702858
version: 1
top level: lacITst
access: public
definition: https://synbiohub.org/public/igem/BBa_E0040/1

BBa_B0010
type: Component
persistent identity: component1702863
display id: component1702863
version: 1
top level: lacITst
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0034
type: Component
persistent identity: component1702806
display id: component1702806
version: 1
top level: lacITst
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0034
type: Component
persistent identity: component1702856
display id: component1702856
version: 1
top level: lacITst
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0012
type: Component
persistent identity: component1702833
display id: component1702833
version: 1
top level: lacITst
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_C0012
type: Component
persistent identity: component1702816
display id: component1702816
version: 1
top level: lacITst
access: public
definition: https://synbiohub.org/public/igem/BBa_C0012/1

BBa_B0010
type: Component
persistent identity: component1702823
display id: component1702823
version: 1
top level: lacITst
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1702798
display id: annotation1702798
version: 1
top level: lacITst
location: https://synbiohub.org/public/igem/BBa_J05505/annotation1702798/range1702798/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_J05505/annotation1702798/range1702798/1
type: Range
persistent identity: range1702798
display id: range1702798
version: 1
direction: direction/forward
top level: lacITst
start: 1
end: 54
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1702863
display id: annotation1702863
version: 1
top level: lacITst
location: https://synbiohub.org/public/igem/BBa_J05505/annotation1702863/range1702863/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_J05505/annotation1702863/range1702863/1
type: Range
persistent identity: range1702863
display id: range1702863
version: 1
direction: direction/forward
top level: lacITst
start: 2188
end: 2267
orientation: inline

BBa_C0012
type: SequenceAnnotation
persistent identity: annotation1702816
display id: annotation1702816
version: 1
top level: lacITst
location: https://synbiohub.org/public/igem/BBa_J05505/annotation1702816/range1702816/1
component: BBa_C0012

https://synbiohub.org/public/igem/BBa_J05505/annotation1702816/range1702816/1
type: Range
persistent identity: range1702816
display id: range1702816
version: 1
direction: direction/forward
top level: lacITst
start: 81
end: 1208
orientation: inline

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation1702847
display id: annotation1702847
version: 1
top level: lacITst
location: https://synbiohub.org/public/igem/BBa_J05505/annotation1702847/range1702847/1
component: BBa_R0011

https://synbiohub.org/public/igem/BBa_J05505/annotation1702847/range1702847/1
type: Range
persistent identity: range1702847
display id: range1702847
version: 1
direction: direction/forward
top level: lacITst
start: 1379
end: 1432
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1702833
display id: annotation1702833
version: 1
top level: lacITst
location: https://synbiohub.org/public/igem/BBa_J05505/annotation1702833/range1702833/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_J05505/annotation1702833/range1702833/1
type: Range
persistent identity: range1702833
display id: range1702833
version: 1
direction: direction/forward
top level: lacITst
start: 1330
end: 1370
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1702806
display id: annotation1702806
version: 1
top level: lacITst
location: https://synbiohub.org/public/igem/BBa_J05505/annotation1702806/range1702806/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_J05505/annotation1702806/range1702806/1
type: Range
persistent identity: range1702806
display id: range1702806
version: 1
direction: direction/forward
top level: lacITst
start: 63
end: 74
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation1702858
display id: annotation1702858
version: 1
top level: lacITst
location: https://synbiohub.org/public/igem/BBa_J05505/annotation1702858/range1702858/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_J05505/annotation1702858/range1702858/1
type: Range
persistent identity: range1702858
display id: range1702858
version: 1
direction: direction/forward
top level: lacITst
start: 1460
end: 2179
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1702873
display id: annotation1702873
version: 1
top level: lacITst
location: https://synbiohub.org/public/igem/BBa_J05505/annotation1702873/range1702873/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_J05505/annotation1702873/range1702873/1
type: Range
persistent identity: range1702873
display id: range1702873
version: 1
direction: direction/forward
top level: lacITst
start: 2276
end: 2316
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1702856
display id: annotation1702856
version: 1
top level: lacITst
location: https://synbiohub.org/public/igem/BBa_J05505/annotation1702856/range1702856/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_J05505/annotation1702856/range1702856/1
type: Range
persistent identity: range1702856
display id: range1702856
version: 1
direction: direction/forward
top level: lacITst
start: 1442
end: 1453
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1702823
display id: annotation1702823
version: 1
top level: lacITst
location: https://synbiohub.org/public/igem/BBa_J05505/annotation1702823/range1702823/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_J05505/annotation1702823/range1702823/1
type: Range
persistent identity: range1702823
display id: range1702823
version: 1
direction: direction/forward
top level: lacITst
start: 1242
end: 1321
orientation: inline

BBa_I7150
type: ComponentDefinition
persistent identity: BBa I7150
display id: BBa_I7150
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: R0040.B0030.E0044.B0015
creator: jcbraff
created: 2004-10-25T11:00:00Z
modified: 2015-08-31T04:07:49Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_1_
m user id: 0
owner id: 61
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: R0040.B0030.E0044.B0015, GFP-AAV expression device
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Measurement
component: BBa_E0044
component: BBa_B0012
component: BBa_R0040
component: BBa_B0010
component: BBa_B0030
sequence annotation: BBa_B0012
sequence annotation: BBa_B0010
sequence annotation: BBa_B0030
sequence annotation: BBa_R0040
sequence annotation: BBa_E0044
sequence: https://synbiohub.org/public/igem/BBa_I7150_sequence/1

BBa_E0044
type: Component
persistent identity: component1250393
display id: component1250393
version: 1
top level: BBa_I7150
access: public
definition: https://synbiohub.org/public/igem/BBa_E0044/1

BBa_B0012
type: Component
persistent identity: component1250408
display id: component1250408
version: 1
top level: BBa_I7150
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_R0040
type: Component
persistent identity: component1250379
display id: component1250379
version: 1
top level: BBa_I7150
access: public
definition: https://synbiohub.org/public/igem/BBa_R0040/1

BBa_B0010
type: Component
persistent identity: component1250398
display id: component1250398
version: 1
top level: BBa_I7150
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0030
type: Component
persistent identity: component1250390
display id: component1250390
version: 1
top level: BBa_I7150
access: public
definition: https://synbiohub.org/public/igem/BBa_B0030/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1250408
display id: annotation1250408
version: 1
top level: BBa_I7150
location: https://synbiohub.org/public/igem/BBa_I7150/annotation1250408/range1250408/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I7150/annotation1250408/range1250408/1
type: Range
persistent identity: range1250408
display id: range1250408
version: 1
direction: direction/forward
top level: BBa_I7150
start: 939
end: 979
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1250398
display id: annotation1250398
version: 1
top level: BBa_I7150
location: https://synbiohub.org/public/igem/BBa_I7150/annotation1250398/range1250398/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I7150/annotation1250398/range1250398/1
type: Range
persistent identity: range1250398
display id: range1250398
version: 1
direction: direction/forward
top level: BBa_I7150
start: 851
end: 930
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation1250390
display id: annotation1250390
version: 1
top level: BBa_I7150
location: https://synbiohub.org/public/igem/BBa_I7150/annotation1250390/range1250390/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_I7150/annotation1250390/range1250390/1
type: Range
persistent identity: range1250390
display id: range1250390
version: 1
direction: direction/forward
top level: BBa_I7150
start: 63
end: 77
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1250379
display id: annotation1250379
version: 1
top level: BBa_I7150
location: https://synbiohub.org/public/igem/BBa_I7150/annotation1250379/range1250379/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_I7150/annotation1250379/range1250379/1
type: Range
persistent identity: range1250379
display id: range1250379
version: 1
direction: direction/forward
top level: BBa_I7150
start: 1
end: 54
orientation: inline

BBa_E0044
type: SequenceAnnotation
persistent identity: annotation1250393
display id: annotation1250393
version: 1
top level: BBa_I7150
location: https://synbiohub.org/public/igem/BBa_I7150/annotation1250393/range1250393/1
component: BBa_E0044

https://synbiohub.org/public/igem/BBa_I7150/annotation1250393/range1250393/1
type: Range
persistent identity: range1250393
display id: range1250393
version: 1
direction: direction/forward
top level: BBa_I7150
start: 84
end: 842
orientation: inline

BBa_M30012
type: ComponentDefinition
persistent identity: BBa M30012
display id: BBa_M30012
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: OmpR controlled mRFP
creator: natalie kuldell
created: 2005-12-14T12:00:00Z
modified: 2015-05-08T01:13:59Z
discontinued: false
dominant: true
experience: experience/None
group u list: _45_1_
m user id: 0
owner id: 314
owning group id: 1
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Positively regulated, OmpR-controlled promoter from OmpC gene (R0082) directing transcription of mRFP (I13507). Constructed with standard assembly method except R0082 was isolated using VF and VR-directed PCR. Consequently, there may be silent mutations that were inadvertently introduced. An alternative clone is M30011. Cells bearing this construct are pale pink.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Measurement
component: BBa_B0010
component: BBa_E1010
component: BBa_B0012
component: BBa_R0082
component: BBa_B0034
sequence annotation: BBa_E1010
sequence annotation: BBa_B0034
sequence annotation: BBa_B0010
sequence annotation: BBa_R0082
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_M30012_sequence/1

BBa_B0010
type: Component
persistent identity: component1769070
display id: component1769070
version: 1
top level: BBa_M30012
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_E1010
type: Component
persistent identity: component1769064
display id: component1769064
version: 1
top level: BBa_M30012
access: public
definition: https://synbiohub.org/public/igem/BBa_E1010/1

BBa_B0012
type: Component
persistent identity: component1769080
display id: component1769080
version: 1
top level: BBa_M30012
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_R0082
type: Component
persistent identity: component1769052
display id: component1769052
version: 1
top level: BBa_M30012
access: public
definition: https://synbiohub.org/public/igem/BBa_R0082/1

BBa_B0034
type: Component
persistent identity: component1769057
display id: component1769057
version: 1
top level: BBa_M30012
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_E1010
type: SequenceAnnotation
persistent identity: annotation1769064
display id: annotation1769064
version: 1
top level: BBa_M30012
location: https://synbiohub.org/public/igem/BBa_M30012/annotation1769064/range1769064/1
component: BBa_E1010

https://synbiohub.org/public/igem/BBa_M30012/annotation1769064/range1769064/1
type: Range
persistent identity: range1769064
display id: range1769064
version: 1
direction: direction/forward
top level: BBa_M30012
start: 135
end: 815
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1769057
display id: annotation1769057
version: 1
top level: BBa_M30012
location: https://synbiohub.org/public/igem/BBa_M30012/annotation1769057/range1769057/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_M30012/annotation1769057/range1769057/1
type: Range
persistent identity: range1769057
display id: range1769057
version: 1
direction: direction/forward
top level: BBa_M30012
start: 117
end: 128
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1769070
display id: annotation1769070
version: 1
top level: BBa_M30012
location: https://synbiohub.org/public/igem/BBa_M30012/annotation1769070/range1769070/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_M30012/annotation1769070/range1769070/1
type: Range
persistent identity: range1769070
display id: range1769070
version: 1
direction: direction/forward
top level: BBa_M30012
start: 849
end: 928
orientation: inline

BBa_R0082
type: SequenceAnnotation
persistent identity: annotation1769052
display id: annotation1769052
version: 1
top level: BBa_M30012
location: https://synbiohub.org/public/igem/BBa_M30012/annotation1769052/range1769052/1
component: BBa_R0082

https://synbiohub.org/public/igem/BBa_M30012/annotation1769052/range1769052/1
type: Range
persistent identity: range1769052
display id: range1769052
version: 1
direction: direction/forward
top level: BBa_M30012
start: 1
end: 108
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1769080
display id: annotation1769080
version: 1
top level: BBa_M30012
location: https://synbiohub.org/public/igem/BBa_M30012/annotation1769080/range1769080/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_M30012/annotation1769080/range1769080/1
type: Range
persistent identity: range1769080
display id: range1769080
version: 1
direction: direction/forward
top level: BBa_M30012
start: 937
end: 977
orientation: inline

AnRe
type: ComponentDefinition
persistent identity: BBa K772006
display id: BBa_K772006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: GFP - LacZ anchor-inducer protein complex
creator: Mustafa Elitok
created: 2012-09-24T11:00:00Z
modified: 2015-05-08T01:13:15Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1024_
m user id: 0
owner id: 9573
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This part contains a green fluorescent protein (GFP) as a non-reactive chemical compound (anchor) in order to neutralize the active reactive fixed to it, a reporter/inducer device or protein (enzyme etc.) and a TEV protease cleavage site between them to assemble these two proteins. BBa_772001 and BBa_K772002 are assembled to be used as the trigger of detection in the whole system which ends with the intracellular transfection (or activation) of TEV protease. After the activation, it is planned that TEV protease will cleave this anchor-inducer protein complex to release free the inducer/reporter. In conclusion, the inducer would activate a transcription pathway on the targeted inducible gene or the reporter would indicate that there is a change in the whole system.
mutable notes: Note that there is an RBS sequence just before the protein sequence in order to eliminate the need of addition of it.
mutable provenance: Cleavage site, LacZ and GFP sequences are gathered from Registry.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
sequence annotation: LacZ
sequence annotation: RBS
sequence annotation: Double Terminator
sequence annotation: GFP
sequence annotation: Anchor - Reporter
sequence annotation: TEV cleavage site
sequence: https://synbiohub.org/public/igem/BBa_K772006_sequence/1

LacZ
type: SequenceAnnotation
persistent identity: annotation2203906
display id: annotation2203906
version: 1
top level: AnRe
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K772006/annotation2203906/range2203906/1

https://synbiohub.org/public/igem/BBa_K772006/annotation2203906/range2203906/1
type: Range
persistent identity: range2203906
display id: range2203906
version: 1
direction: direction/forward
top level: AnRe
start: 832
end: 1075
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2203903
display id: annotation2203903
version: 1
top level: AnRe
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K772006/annotation2203903/range2203903/1

https://synbiohub.org/public/igem/BBa_K772006/annotation2203903/range2203903/1
type: Range
persistent identity: range2203903
display id: range2203903
version: 1
direction: direction/forward
top level: AnRe
start: 1
end: 20
orientation: inline

Double Terminator
type: SequenceAnnotation
persistent identity: annotation2203907
display id: annotation2203907
version: 1
top level: AnRe
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K772006/annotation2203907/range2203907/1

https://synbiohub.org/public/igem/BBa_K772006/annotation2203907/range2203907/1
type: Range
persistent identity: range2203907
display id: range2203907
version: 1
direction: direction/forward
top level: AnRe
start: 1076
end: 1207
orientation: inline

GFP
type: SequenceAnnotation
persistent identity: annotation2203904
display id: annotation2203904
version: 1
top level: AnRe
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K772006/annotation2203904/range2203904/1

https://synbiohub.org/public/igem/BBa_K772006/annotation2203904/range2203904/1
type: Range
persistent identity: range2203904
display id: range2203904
version: 1
direction: direction/forward
top level: AnRe
start: 21
end: 741
orientation: inline

Anchor - Reporter
type: SequenceAnnotation
persistent identity: annotation2203908
display id: annotation2203908
version: 1
top level: AnRe
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K772006/annotation2203908/range2203908/1

https://synbiohub.org/public/igem/BBa_K772006/annotation2203908/range2203908/1
type: Range
persistent identity: range2203908
display id: range2203908
version: 1
direction: direction/forward
top level: AnRe
start: 21
end: 1075
orientation: inline

TEV cleavage site
type: SequenceAnnotation
persistent identity: annotation2203905
display id: annotation2203905
version: 1
top level: AnRe
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K772006/annotation2203905/range2203905/1

https://synbiohub.org/public/igem/BBa_K772006/annotation2203905/range2203905/1
type: Range
persistent identity: range2203905
display id: range2203905
version: 1
direction: direction/forward
top level: AnRe
start: 742
end: 831
orientation: inline

BBa_I13102
type: ComponentDefinition
persistent identity: BBa I13102
display id: BBa_I13102
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Lac Self-coupled Inverter
creator: Barry Canton
created: 2004-07-20T11:00:00Z
modified: 2015-08-31T04:07:33Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_6_
m user id: 0
owner id: 135
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Measurement
component: BBa_R0011
component: BBa_I13021
sequence annotation: BBa_R0011
sequence annotation: BBa_I13021
sequence: https://synbiohub.org/public/igem/BBa_I13102_sequence/1

BBa_R0011
type: Component
persistent identity: component2229145
display id: component2229145
version: 1
top level: BBa_I13102
access: public
definition: https://synbiohub.org/public/igem/BBa_R0011/1

BBa_I13021
type: Component
persistent identity: component2229179
display id: component2229179
version: 1
top level: BBa_I13102
access: public
definition: https://synbiohub.org/public/igem/BBa_I13021/1

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation2229145
display id: annotation2229145
version: 1
top level: BBa_I13102
location: https://synbiohub.org/public/igem/BBa_I13102/annotation2229145/range2229145/1
component: BBa_R0011

https://synbiohub.org/public/igem/BBa_I13102/annotation2229145/range2229145/1
type: Range
persistent identity: range2229145
display id: range2229145
version: 1
direction: direction/forward
top level: BBa_I13102
start: 1
end: 54
orientation: inline

BBa_I13021
type: SequenceAnnotation
persistent identity: annotation2229179
display id: annotation2229179
version: 1
top level: BBa_I13102
location: https://synbiohub.org/public/igem/BBa_I13102/annotation2229179/range2229179/1
component: BBa_I13021

https://synbiohub.org/public/igem/BBa_I13102/annotation2229179/range2229179/1
type: Range
persistent identity: range2229179
display id: range2229179
version: 1
direction: direction/reverse
top level: BBa_I13102
start: 64
end: 2320
orientation: inline

BBa_I13654
type: ComponentDefinition
persistent identity: BBa I13654
display id: BBa_I13654
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Cascaded Tet (medium) and Lac (medium) QPIs with constitutive promoter as input
creator: mit
created: 2004-07-19T11:00:00Z
modified: 2015-08-31T04:07:36Z
discontinued: false
dominant: false
experience: experience/None
group u list: _6_
m user id: 0
owner id: 101
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Measurement
component: BBa_R0051
component: BBa_Q01400
component: BBa_Q01121
sequence annotation: BBa_Q01121
sequence annotation: BBa_R0051
sequence annotation: BBa_Q01400
sequence: https://synbiohub.org/public/igem/BBa_I13654_sequence/1

BBa_R0051
type: Component
persistent identity: component2231336
display id: component2231336
version: 1
top level: BBa_I13654
access: public
definition: https://synbiohub.org/public/igem/BBa_R0051/1

BBa_Q01400
type: Component
persistent identity: component2231357
display id: component2231357
version: 1
top level: BBa_I13654
access: public
definition: https://synbiohub.org/public/igem/BBa_Q01400/1

BBa_Q01121
type: Component
persistent identity: component2231376
display id: component2231376
version: 1
top level: BBa_I13654
access: public
definition: https://synbiohub.org/public/igem/BBa_Q01121/1

BBa_Q01121
type: SequenceAnnotation
persistent identity: annotation2231376
display id: annotation2231376
version: 1
top level: BBa_I13654
location: https://synbiohub.org/public/igem/BBa_I13654/annotation2231376/range2231376/1
component: BBa_Q01121

https://synbiohub.org/public/igem/BBa_I13654/annotation2231376/range2231376/1
type: Range
persistent identity: range2231376
display id: range2231376
version: 1
direction: direction/reverse
top level: BBa_I13654
start: 970
end: 2342
orientation: inline

BBa_R0051
type: SequenceAnnotation
persistent identity: annotation2231336
display id: annotation2231336
version: 1
top level: BBa_I13654
location: https://synbiohub.org/public/igem/BBa_I13654/annotation2231336/range2231336/1
component: BBa_R0051

https://synbiohub.org/public/igem/BBa_I13654/annotation2231336/range2231336/1
type: Range
persistent identity: range2231336
display id: range2231336
version: 1
direction: direction/forward
top level: BBa_I13654
start: 1
end: 49
orientation: inline

BBa_Q01400
type: SequenceAnnotation
persistent identity: annotation2231357
display id: annotation2231357
version: 1
top level: BBa_I13654
location: https://synbiohub.org/public/igem/BBa_I13654/annotation2231357/range2231357/1
component: BBa_Q01400

https://synbiohub.org/public/igem/BBa_I13654/annotation2231357/range2231357/1
type: Range
persistent identity: range2231357
display id: range2231357
version: 1
direction: direction/reverse
top level: BBa_I13654
start: 58
end: 961
orientation: inline

BBa_I12242
type: ComponentDefinition
persistent identity: BBa I12242
display id: BBa_I12242
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Test of AraC promoter region
creator: rwald
created: 2004-08-31T11:00:00Z
modified: 2015-08-31T04:07:32Z
discontinued: false
dominant: false
experience: experience/None
group u list: _3_
m user id: 0
owner id: 120
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This component tests the BBa_R0080 AraC-induced promoter region.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Measurement
component: BBa_B0034
component: BBa_B0010
component: BBa_B0012
component: BBa_R0080
component: BBa_E0022
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_R0080
sequence annotation: BBa_E0022
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_I12242_sequence/1

BBa_B0034
type: Component
persistent identity: component1062915
display id: component1062915
version: 1
top level: BBa_I12242
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0010
type: Component
persistent identity: component1062935
display id: component1062935
version: 1
top level: BBa_I12242
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0012
type: Component
persistent identity: component1062945
display id: component1062945
version: 1
top level: BBa_I12242
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_R0080
type: Component
persistent identity: component1062910
display id: component1062910
version: 1
top level: BBa_I12242
access: public
definition: https://synbiohub.org/public/igem/BBa_R0080/1

BBa_E0022
type: Component
persistent identity: component1062927
display id: component1062927
version: 1
top level: BBa_I12242
access: public
definition: https://synbiohub.org/public/igem/BBa_E0022/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1062935
display id: annotation1062935
version: 1
top level: BBa_I12242
location: https://synbiohub.org/public/igem/BBa_I12242/annotation1062935/range1062935/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I12242/annotation1062935/range1062935/1
type: Range
persistent identity: range1062935
display id: range1062935
version: 1
direction: direction/forward
top level: BBa_I12242
start: 946
end: 1025
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1062945
display id: annotation1062945
version: 1
top level: BBa_I12242
location: https://synbiohub.org/public/igem/BBa_I12242/annotation1062945/range1062945/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I12242/annotation1062945/range1062945/1
type: Range
persistent identity: range1062945
display id: range1062945
version: 1
direction: direction/forward
top level: BBa_I12242
start: 1034
end: 1074
orientation: inline

BBa_R0080
type: SequenceAnnotation
persistent identity: annotation1062910
display id: annotation1062910
version: 1
top level: BBa_I12242
location: https://synbiohub.org/public/igem/BBa_I12242/annotation1062910/range1062910/1
component: BBa_R0080

https://synbiohub.org/public/igem/BBa_I12242/annotation1062910/range1062910/1
type: Range
persistent identity: range1062910
display id: range1062910
version: 1
direction: direction/forward
top level: BBa_I12242
start: 1
end: 149
orientation: inline

BBa_E0022
type: SequenceAnnotation
persistent identity: annotation1062927
display id: annotation1062927
version: 1
top level: BBa_I12242
location: https://synbiohub.org/public/igem/BBa_I12242/annotation1062927/range1062927/1
component: BBa_E0022

https://synbiohub.org/public/igem/BBa_I12242/annotation1062927/range1062927/1
type: Range
persistent identity: range1062927
display id: range1062927
version: 1
direction: direction/forward
top level: BBa_I12242
start: 176
end: 937
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1062915
display id: annotation1062915
version: 1
top level: BBa_I12242
location: https://synbiohub.org/public/igem/BBa_I12242/annotation1062915/range1062915/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I12242/annotation1062915/range1062915/1
type: Range
persistent identity: range1062915
display id: range1062915
version: 1
direction: direction/forward
top level: BBa_I12242
start: 158
end: 169
orientation: inline