//classic/other
type: Collection
persistent identity: classic other
display id: classic_other
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
member: //classic/other/uncategorized

//classic/other/uncategorized
type: Collection
persistent identity: classic other uncategorized
display id: classic_other_uncategorized
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
member: BBa_Y1010
member: P22 attB
member: BBa_Y1002
member: BBa_J07003
member: BBa_I11053
member: BBa_J07029
member: BBa_P0001
member: BBa_Y1007
member: BBa_Y1011
member: Message
member: LVA
member: spacer
member: trkD
member: cI-ptetR
member: BBa_Y1014
member: BBa_I11052
member: Lambda att
member: BBa_Y1006
member: BBa_J07002
member: BBa_I11043
member: BBa_Y1009
member: BBa_J07006
member: BBa_Y1004
member: Homol.
member: BBa_Y1005
member: BBa_J07031
member: BBa_I11042
member: BBa_J07001
member: BBa_Y1003
member: BBa_Y1016
member: BBa_Y1008
member: P22 attP
member: BBa_J07004
member: BBa_Y1012
member: SSRa
member: BBa_J07000

BBa_Y1002
type: ComponentDefinition
persistent identity: BBa Y1002
display id: BBa_Y1002
version: 1
was generated by: iGEM to SBOL conversion
description: Ampicillian Resistance Gene
created: 2005-06-20T11:00:00Z
modified: 2015-05-08T01:14:54Z
owned by: james
owned by: myers
mutable description: Has its own promoter and terminator
discontinued: false
dominant: true
experience: experience/None
group u list: _11_
m user id: 0
owner id: 351
owning group id: 43
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Samantha Sutton
type: DnaRegion
role: partType/Other
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_Y1002_sequence/1

BBa_Y1004
type: ComponentDefinition
persistent identity: BBa Y1004
display id: BBa_Y1004
version: 1
was generated by: iGEM to SBOL conversion
description: Cen/Ars
created: 2005-06-22T11:00:00Z
modified: 2015-05-08T01:14:54Z
owned by: james
owned by: myers
mutable description: Yeast origin
discontinued: false
dominant: false
experience: experience/None
group u list: _11_
m user id: 0
owner id: 351
owning group id: 43
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Samantha Sutton
type: DnaRegion
role: partType/Other
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_Y1004_sequence/1

BBa_P0001
type: ComponentDefinition
persistent identity: BBa P0001
display id: BBa_P0001
version: 1
was generated by: iGEM to SBOL conversion
description: Crackparts
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:10Z
owned by: james
owned by: myers
mutable description: Initial design for crackpots bullseye
discontinued: true
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: Discontinued
status: status/Deleted
bookmark: false
star: false
type: DnaRegion
role: partType/Other
role: SO:0000110

BBa_J07003
type: ComponentDefinition
persistent identity: BBa J07003
display id: BBa_J07003
version: 1
was generated by: iGEM to SBOL conversion
description: abandoned test construct
created: 2005-06-15T11:00:00Z
modified: 2015-08-31T04:08:19Z
owned by: james
owned by: myers
discontinued: false
dominant: false
experience: experience/None
group u list: _21_
m user id: 0
owner id: 350
owning group id: 21
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Jenny
type: DnaRegion
role: partType/Other
role: SO:0000110
component: BBa_E0030
component: BBa_C0012
component: BBa_B0034
component: BBa_B0012
component: BBa_C0051
component: BBa_Z0251
component: BBa_R0051
component: BBa_B0010
component: BBa_R0011
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_C0012
sequence annotation: BBa_B0034
sequence annotation: BBa_R0011
sequence annotation: BBa_R0051
sequence annotation: BBa_Z0251
sequence annotation: BBa_E0030
sequence annotation: BBa_C0051
sequence: https://synbiohub.org/public/igem/BBa_J07003_sequence/1

BBa_E0030
type: Component
persistent identity: component1522470
display id: component1522470
version: 1
top level: BBa_J07003
access: public
definition: eyfp

BBa_C0012
type: Component
persistent identity: component1522379
display id: component1522379
version: 1
top level: BBa_J07003
access: public
definition: lacI

BBa_B0034
type: Component
persistent identity: component1522369
display id: component1522369
version: 1
top level: BBa_J07003
access: public
definition: BBa_B0034

BBa_B0012
type: Component
persistent identity: component1522396
display id: component1522396
version: 1
top level: BBa_J07003
access: public
definition: BBa_B0012

BBa_C0051
type: Component
persistent identity: component1522429
display id: component1522429
version: 1
top level: BBa_J07003
access: public
definition: cI lam

BBa_B0012
type: Component
persistent identity: component1522445
display id: component1522445
version: 1
top level: BBa_J07003
access: public
definition: BBa_B0012

BBa_Z0251
type: Component
persistent identity: component1522364
display id: component1522364
version: 1
top level: BBa_J07003
access: public
definition: BBa_Z0251

BBa_R0051
type: Component
persistent identity: component1522460
display id: component1522460
version: 1
top level: BBa_J07003
access: public
definition: cI lam

BBa_B0012
type: Component
persistent identity: component1522485
display id: component1522485
version: 1
top level: BBa_J07003
access: public
definition: BBa_B0012

BBa_B0034
type: Component
persistent identity: component1522468
display id: component1522468
version: 1
top level: BBa_J07003
access: public
definition: BBa_B0034

BBa_B0034
type: Component
persistent identity: component1522419
display id: component1522419
version: 1
top level: BBa_J07003
access: public
definition: BBa_B0034

BBa_B0010
type: Component
persistent identity: component1522435
display id: component1522435
version: 1
top level: BBa_J07003
access: public
definition: BBa_B0010

BBa_R0011
type: Component
persistent identity: component1522410
display id: component1522410
version: 1
top level: BBa_J07003
access: public
definition: lacI+pL

BBa_B0010
type: Component
persistent identity: component1522386
display id: component1522386
version: 1
top level: BBa_J07003
access: public
definition: BBa_B0010

BBa_B0010
type: Component
persistent identity: component1522475
display id: component1522475
version: 1
top level: BBa_J07003
access: public
definition: BBa_B0010

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1522475
display id: annotation1522475
version: 1
top level: BBa_J07003
location: https://synbiohub.org/public/igem/BBa_J07003/annotation1522475/range1522475/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_J07003/annotation1522475/range1522475/1
type: Range
persistent identity: range1522475
display id: range1522475
version: 1
top level: BBa_J07003
direction: direction/forward
start: 3167
end: 3246
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1522485
display id: annotation1522485
version: 1
top level: BBa_J07003
location: https://synbiohub.org/public/igem/BBa_J07003/annotation1522485/range1522485/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_J07003/annotation1522485/range1522485/1
type: Range
persistent identity: range1522485
display id: range1522485
version: 1
top level: BBa_J07003
direction: direction/forward
start: 3255
end: 3295
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1522445
display id: annotation1522445
version: 1
top level: BBa_J07003
location: https://synbiohub.org/public/igem/BBa_J07003/annotation1522445/range1522445/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_J07003/annotation1522445/range1522445/1
type: Range
persistent identity: range1522445
display id: range1522445
version: 1
top level: BBa_J07003
direction: direction/forward
start: 2312
end: 2352
orientation: inline

BBa_C0012
type: SequenceAnnotation
persistent identity: annotation1522379
display id: annotation1522379
version: 1
top level: BBa_J07003
location: https://synbiohub.org/public/igem/BBa_J07003/annotation1522379/range1522379/1
component: BBa_C0012

https://synbiohub.org/public/igem/BBa_J07003/annotation1522379/range1522379/1
type: Range
persistent identity: range1522379
display id: range1522379
version: 1
top level: BBa_J07003
direction: direction/forward
start: 62
end: 1189
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1522468
display id: annotation1522468
version: 1
top level: BBa_J07003
location: https://synbiohub.org/public/igem/BBa_J07003/annotation1522468/range1522468/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_J07003/annotation1522468/range1522468/1
type: Range
persistent identity: range1522468
display id: range1522468
version: 1
top level: BBa_J07003
direction: direction/forward
start: 2418
end: 2429
orientation: inline

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation1522410
display id: annotation1522410
version: 1
top level: BBa_J07003
location: https://synbiohub.org/public/igem/BBa_J07003/annotation1522410/range1522410/1
component: BBa_R0011

https://synbiohub.org/public/igem/BBa_J07003/annotation1522410/range1522410/1
type: Range
persistent identity: range1522410
display id: range1522410
version: 1
top level: BBa_J07003
direction: direction/forward
start: 1360
end: 1413
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1522369
display id: annotation1522369
version: 1
top level: BBa_J07003
location: https://synbiohub.org/public/igem/BBa_J07003/annotation1522369/range1522369/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_J07003/annotation1522369/range1522369/1
type: Range
persistent identity: range1522369
display id: range1522369
version: 1
top level: BBa_J07003
direction: direction/forward
start: 44
end: 55
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1522396
display id: annotation1522396
version: 1
top level: BBa_J07003
location: https://synbiohub.org/public/igem/BBa_J07003/annotation1522396/range1522396/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_J07003/annotation1522396/range1522396/1
type: Range
persistent identity: range1522396
display id: range1522396
version: 1
top level: BBa_J07003
direction: direction/forward
start: 1311
end: 1351
orientation: inline

BBa_R0051
type: SequenceAnnotation
persistent identity: annotation1522460
display id: annotation1522460
version: 1
top level: BBa_J07003
location: https://synbiohub.org/public/igem/BBa_J07003/annotation1522460/range1522460/1
component: BBa_R0051

https://synbiohub.org/public/igem/BBa_J07003/annotation1522460/range1522460/1
type: Range
persistent identity: range1522460
display id: range1522460
version: 1
top level: BBa_J07003
direction: direction/forward
start: 2361
end: 2409
orientation: inline

BBa_Z0251
type: SequenceAnnotation
persistent identity: annotation1522364
display id: annotation1522364
version: 1
top level: BBa_J07003
location: https://synbiohub.org/public/igem/BBa_J07003/annotation1522364/range1522364/1
component: BBa_Z0251

https://synbiohub.org/public/igem/BBa_J07003/annotation1522364/range1522364/1
type: Range
persistent identity: range1522364
display id: range1522364
version: 1
top level: BBa_J07003
direction: direction/forward
start: 1
end: 35
orientation: inline

BBa_E0030
type: SequenceAnnotation
persistent identity: annotation1522470
display id: annotation1522470
version: 1
top level: BBa_J07003
location: https://synbiohub.org/public/igem/BBa_J07003/annotation1522470/range1522470/1
component: BBa_E0030

https://synbiohub.org/public/igem/BBa_J07003/annotation1522470/range1522470/1
type: Range
persistent identity: range1522470
display id: range1522470
version: 1
top level: BBa_J07003
direction: direction/forward
start: 2436
end: 3158
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1522419
display id: annotation1522419
version: 1
top level: BBa_J07003
location: https://synbiohub.org/public/igem/BBa_J07003/annotation1522419/range1522419/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_J07003/annotation1522419/range1522419/1
type: Range
persistent identity: range1522419
display id: range1522419
version: 1
top level: BBa_J07003
direction: direction/forward
start: 1423
end: 1434
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1522386
display id: annotation1522386
version: 1
top level: BBa_J07003
location: https://synbiohub.org/public/igem/BBa_J07003/annotation1522386/range1522386/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_J07003/annotation1522386/range1522386/1
type: Range
persistent identity: range1522386
display id: range1522386
version: 1
top level: BBa_J07003
direction: direction/forward
start: 1223
end: 1302
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1522435
display id: annotation1522435
version: 1
top level: BBa_J07003
location: https://synbiohub.org/public/igem/BBa_J07003/annotation1522435/range1522435/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_J07003/annotation1522435/range1522435/1
type: Range
persistent identity: range1522435
display id: range1522435
version: 1
top level: BBa_J07003
direction: direction/forward
start: 2224
end: 2303
orientation: inline

BBa_C0051
type: SequenceAnnotation
persistent identity: annotation1522429
display id: annotation1522429
version: 1
top level: BBa_J07003
location: https://synbiohub.org/public/igem/BBa_J07003/annotation1522429/range1522429/1
component: BBa_C0051

https://synbiohub.org/public/igem/BBa_J07003/annotation1522429/range1522429/1
type: Range
persistent identity: range1522429
display id: range1522429
version: 1
top level: BBa_J07003
direction: direction/forward
start: 1441
end: 2190
orientation: inline

LVA
type: ComponentDefinition
persistent identity: BBa I14070
display id: BBa_I14070
version: 1
was generated by: iGEM to SBOL conversion
description: LVA tag
created: 2004-08-04T11:00:00Z
modified: 2015-08-31T04:07:38Z
owned by: james
owned by: myers
mutable description: -- No description --
discontinued: true
dominant: false
experience: experience/None
group u list: _4_
m user id: 0
owner id: 166
owning group id: 7
sample status: Discontinued
status: status/Deleted
bookmark: false
star: false
creator: JAS
type: DnaRegion
role: partType/Other
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_I14070_sequence/1

SSRa
type: ComponentDefinition
persistent identity: BBa I14071
display id: BBa_I14071
version: 1
was generated by: iGEM to SBOL conversion
description: SSRa tail
created: 2004-08-04T11:00:00Z
modified: 2015-08-31T04:07:38Z
owned by: james
owned by: myers
mutable description: -- No description --
discontinued: true
dominant: false
experience: experience/None
group u list: _4_
m user id: 0
owner id: 166
owning group id: 7
sample status: Discontinued
status: status/Deleted
bookmark: false
star: false
creator: JAS
type: DnaRegion
role: partType/Other
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_I14071_sequence/1

cI-ptetR
type: ComponentDefinition
persistent identity: BBa I1010
display id: BBa_I1010
version: 1
was generated by: iGEM to SBOL conversion
description: cI(1) fused to tetR promoter
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:29Z
mutable provenance: Lutz, R., Bujard, H., Nucleic Acids Research (1997) 25, 1203-1210 A synthetic oscillatory network of transcriptional regulators , Elowitz M.B. , Leibler S., Nature(403),335-38: 2000
owned by: james
owned by: myers
mutable description: This part has been optimized for anti-sense inhibition. It is inhibited by anti-sense parts <bb_part>BBa_I1011</bb_part>, <bb_part>BBa_I1012</bb_part>, and <bb_part>BBa_I1013</bb_part>. It contains the following regions: tetR promoter, anti-sense binding region, RBS, and cI protein with modified initial codons.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: References (unparsed) here: A synthetic oscillatory network of transcriptional regulators , Elowitz M.B. , Leibler S., Nature(403),335-38: 2000 Coleman, J., et al. Nature. (1985) 315, 601-3. Coleman, J., et al. Cell (1984) 37, 429-36. Pestka, S., et al. Proc. Natl. Acad. Sci. USA (1984) 81, 7525-28. Jain, C. (1995). IS10 Antisense Control in Vivo is Affected by Mutations Throughout the Region of Complementarity Between the Interacting RNAs. J. Mol. Biol. 246:585-594. Kittle, J.D., Simons, R.W., Lee, J., and Kleckner, N. (1989). Insertion Sequence IS10 Anti-sense Pairing Initiates by an Interaction Between the 5' End of the Target RNA and a Loop in the Anti-sense RNA. J. Mol. Biol. 210:561-572. Jain, C. (1997). Models for Pairing of IS10 Encoded Antisense RNAs in vivo. J. theor. Biol. 186: 431-439. E. coli codon usage table at http://bioinfo.weizmann.ac.il:3456/kegg/codon_table/codon_eco.html. Lutz, R., Bujard, H., Nucleic Acids Research (1997) 25, 1203-1210 Mizuno, T., et al. Proc. Natl. Acad. Sci. USA (1984) 81, 1966-1970. References (unparsed) here: A synthetic oscillatory network of transcriptional regulators , Elowitz M.B. , Leibler S., Nature(403),335-38: 2000 Coleman, J., et al. Nature. (1985) 315, 601-3. Coleman, J., et al. Cell (1984) 37, 429-36. Pestka, S., et al. Proc. Natl. Acad. Sci. USA (1984) 81, 7525-28. Jain, C. (1995). IS10 Antisense Control in Vivo is Affected by Mutations Throughout the Region of Complementarity Between the Interacting RNAs. J. Mol. Biol. 246:585-594. Kittle, J.D., Simons, R.W., Lee, J., and Kleckner, N. (1989). Insertion Sequence IS10 Anti-sense Pairing Initiates by an Interaction Between the 5' End of the Target RNA and a Loop in the Anti-sense RNA. J. Mol. Biol. 210:561-572. Jain, C. (1997). Models for Pairing of IS10 Encoded Antisense RNAs in vivo. J. theor. Biol. 186: 431-439. E. coli codon usage table at http://bioinfo.weizmann.ac.il:3456/kegg/codon_table/codon_eco.html. Lutz, R., Bujard, H., Nucleic Acids Research (1997) 25…
star: false
creator: June Rhee, Connie Tao, Ty Thomson, Louis Waldman
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: BBa_I1010
sequence annotation: Modified cI cds
sequence annotation: tetR
sequence annotation: -10
sequence annotation: 2
sequence annotation: Complementary to Antisense mRNA
sequence annotation: cI
sequence annotation: -35
sequence annotation: rbs
sequence annotation: start
sequence annotation: SsrA
sequence annotation: TetR 2
sequence annotation: TetR 1
sequence annotation: added codon (Cys)
sequence: https://synbiohub.org/public/igem/BBa_I1010_sequence/1

BBa_I1010
type: SequenceAnnotation
persistent identity: annotation7042
display id: annotation7042
version: 1
top level: cI-ptetR
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_I1010/annotation7042/range7042/1

https://synbiohub.org/public/igem/BBa_I1010/annotation7042/range7042/1
type: Range
persistent identity: range7042
display id: range7042
version: 1
top level: cI-ptetR
direction: direction/forward
start: 1
end: 834
orientation: inline

Modified cI cds
type: SequenceAnnotation
persistent identity: annotation1783
display id: annotation1783
version: 1
top level: cI-ptetR
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_I1010/annotation1783/range1783/1

https://synbiohub.org/public/igem/BBa_I1010/annotation1783/range1783/1
type: Range
persistent identity: range1783
display id: range1783
version: 1
top level: cI-ptetR
direction: direction/forward
start: 85
end: 157
orientation: inline

tetR
type: SequenceAnnotation
persistent identity: annotation1789
display id: annotation1789
version: 1
top level: cI-ptetR
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I1010/annotation1789/range1789/1

https://synbiohub.org/public/igem/BBa_I1010/annotation1789/range1789/1
type: Range
persistent identity: range1789
display id: range1789
version: 1
top level: cI-ptetR
direction: direction/forward
start: 1
end: 54
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1787
display id: annotation1787
version: 1
top level: cI-ptetR
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I1010/annotation1787/range1787/1

https://synbiohub.org/public/igem/BBa_I1010/annotation1787/range1787/1
type: Range
persistent identity: range1787
display id: range1787
version: 1
top level: cI-ptetR
direction: direction/forward
start: 43
end: 48
orientation: inline

2
type: SequenceAnnotation
persistent identity: annotation1791
display id: annotation1791
version: 1
top level: cI-ptetR
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_I1010/annotation1791/range1791/1

https://synbiohub.org/public/igem/BBa_I1010/annotation1791/range1791/1
type: Range
persistent identity: range1791
display id: range1791
version: 1
top level: cI-ptetR
direction: direction/forward
start: 829
end: 834
orientation: inline

Complementary to Antisense mRNA
type: SequenceAnnotation
persistent identity: annotation1793
display id: annotation1793
version: 1
top level: cI-ptetR
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I1010/annotation1793/range1793/1

https://synbiohub.org/public/igem/BBa_I1010/annotation1793/range1793/1
type: Range
persistent identity: range1793
display id: range1793
version: 1
top level: cI-ptetR
direction: direction/forward
start: 55
end: 157
orientation: inline

cI
type: SequenceAnnotation
persistent identity: annotation1780
display id: annotation1780
version: 1
top level: cI-ptetR
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_I1010/annotation1780/range1780/1

https://synbiohub.org/public/igem/BBa_I1010/annotation1780/range1780/1
type: Range
persistent identity: range1780
display id: range1780
version: 1
top level: cI-ptetR
direction: direction/forward
start: 82
end: 834
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1785
display id: annotation1785
version: 1
top level: cI-ptetR
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I1010/annotation1785/range1785/1

https://synbiohub.org/public/igem/BBa_I1010/annotation1785/range1785/1
type: Range
persistent identity: range1785
display id: range1785
version: 1
top level: cI-ptetR
direction: direction/forward
start: 20
end: 25
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation1782
display id: annotation1782
version: 1
top level: cI-ptetR
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_I1010/annotation1782/range1782/1

https://synbiohub.org/public/igem/BBa_I1010/annotation1782/range1782/1
type: Range
persistent identity: range1782
display id: range1782
version: 1
top level: cI-ptetR
direction: direction/forward
start: 72
end: 77
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation1788
display id: annotation1788
version: 1
top level: cI-ptetR
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_I1010/annotation1788/range1788/1

https://synbiohub.org/public/igem/BBa_I1010/annotation1788/range1788/1
type: Range
persistent identity: range1788
display id: range1788
version: 1
top level: cI-ptetR
direction: direction/forward
start: 55
end: 55
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation1781
display id: annotation1781
version: 1
top level: cI-ptetR
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_I1010/annotation1781/range1781/1

https://synbiohub.org/public/igem/BBa_I1010/annotation1781/range1781/1
type: Range
persistent identity: range1781
display id: range1781
version: 1
top level: cI-ptetR
direction: direction/forward
start: 82
end: 84
orientation: inline

SsrA
type: SequenceAnnotation
persistent identity: annotation1790
display id: annotation1790
version: 1
top level: cI-ptetR
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_I1010/annotation1790/range1790/1

https://synbiohub.org/public/igem/BBa_I1010/annotation1790/range1790/1
type: Range
persistent identity: range1790
display id: range1790
version: 1
top level: cI-ptetR
direction: direction/forward
start: 796
end: 834
orientation: inline

TetR 2
type: SequenceAnnotation
persistent identity: annotation1786
display id: annotation1786
version: 1
top level: cI-ptetR
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I1010/annotation1786/range1786/1

https://synbiohub.org/public/igem/BBa_I1010/annotation1786/range1786/1
type: Range
persistent identity: range1786
display id: range1786
version: 1
top level: cI-ptetR
direction: direction/forward
start: 26
end: 44
orientation: inline

TetR 1
type: SequenceAnnotation
persistent identity: annotation1784
display id: annotation1784
version: 1
top level: cI-ptetR
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I1010/annotation1784/range1784/1

https://synbiohub.org/public/igem/BBa_I1010/annotation1784/range1784/1
type: Range
persistent identity: range1784
display id: range1784
version: 1
top level: cI-ptetR
direction: direction/forward
start: 1
end: 19
orientation: inline

added codon (Cys)
type: SequenceAnnotation
persistent identity: annotation1794
display id: annotation1794
version: 1
top level: cI-ptetR
role: feature/mutation
role: SO:0001059
location: https://synbiohub.org/public/igem/BBa_I1010/annotation1794/range1794/1

https://synbiohub.org/public/igem/BBa_I1010/annotation1794/range1794/1
type: Range
persistent identity: range1794
display id: range1794
version: 1
top level: cI-ptetR
direction: direction/forward
start: 85
end: 87
orientation: inline

BBa_J07004
type: ComponentDefinition
persistent identity: BBa J07004
display id: BBa_J07004
version: 1
was generated by: iGEM to SBOL conversion
description: abandoned test construct
created: 2005-06-15T11:00:00Z
modified: 2015-08-31T04:08:19Z
owned by: james
owned by: myers
discontinued: false
dominant: false
experience: experience/None
group u list: _21_
m user id: 0
owner id: 350
owning group id: 21
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Jenny
type: DnaRegion
role: partType/Other
role: SO:0000110
component: BBa_B0010
component: BBa_R0011
component: BBa_B0012
component: BBa_B0034
component: BBa_R0040
component: BBa_E0020
component: BBa_Z0251
component: BBa_C0012
component: BBa_C0040
sequence annotation: BBa_C0012
sequence annotation: BBa_B0034
sequence annotation: BBa_R0011
sequence annotation: BBa_B0010
sequence annotation: BBa_Z0251
sequence annotation: BBa_R0040
sequence annotation: BBa_B0012
sequence annotation: BBa_E0020
sequence annotation: BBa_C0040
sequence: https://synbiohub.org/public/igem/BBa_J07004_sequence/1

BBa_B0010
type: Component
persistent identity: component1523123
display id: component1523123
version: 1
top level: BBa_J07004
access: public
definition: BBa_B0010

BBa_R0011
type: Component
persistent identity: component1523098
display id: component1523098
version: 1
top level: BBa_J07004
access: public
definition: lacI+pL

BBa_B0012
type: Component
persistent identity: component1523084
display id: component1523084
version: 1
top level: BBa_J07004
access: public
definition: BBa_B0012

BBa_B0010
type: Component
persistent identity: component1523163
display id: component1523163
version: 1
top level: BBa_J07004
access: public
definition: BBa_B0010

BBa_B0034
type: Component
persistent identity: component1523156
display id: component1523156
version: 1
top level: BBa_J07004
access: public
definition: BBa_B0034

BBa_R0040
type: Component
persistent identity: component1523148
display id: component1523148
version: 1
top level: BBa_J07004
access: public
definition: p(tetR)

BBa_B0012
type: Component
persistent identity: component1523133
display id: component1523133
version: 1
top level: BBa_J07004
access: public
definition: BBa_B0012

BBa_E0020
type: Component
persistent identity: component1523158
display id: component1523158
version: 1
top level: BBa_J07004
access: public
definition: ecfp

BBa_Z0251
type: Component
persistent identity: component1523052
display id: component1523052
version: 1
top level: BBa_J07004
access: public
definition: BBa_Z0251

BBa_B0010
type: Component
persistent identity: component1523074
display id: component1523074
version: 1
top level: BBa_J07004
access: public
definition: BBa_B0010

BBa_B0012
type: Component
persistent identity: component1523173
display id: component1523173
version: 1
top level: BBa_J07004
access: public
definition: BBa_B0012

BBa_B0034
type: Component
persistent identity: component1523107
display id: component1523107
version: 1
top level: BBa_J07004
access: public
definition: BBa_B0034

BBa_B0034
type: Component
persistent identity: component1523057
display id: component1523057
version: 1
top level: BBa_J07004
access: public
definition: BBa_B0034

BBa_C0012
type: Component
persistent identity: component1523067
display id: component1523067
version: 1
top level: BBa_J07004
access: public
definition: lacI

BBa_C0040
type: Component
persistent identity: component1523117
display id: component1523117
version: 1
top level: BBa_J07004
access: public
definition: tetR

BBa_C0012
type: SequenceAnnotation
persistent identity: annotation1523067
display id: annotation1523067
version: 1
top level: BBa_J07004
location: https://synbiohub.org/public/igem/BBa_J07004/annotation1523067/range1523067/1
component: BBa_C0012

https://synbiohub.org/public/igem/BBa_J07004/annotation1523067/range1523067/1
type: Range
persistent identity: range1523067
display id: range1523067
version: 1
top level: BBa_J07004
direction: direction/forward
start: 62
end: 1189
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1523107
display id: annotation1523107
version: 1
top level: BBa_J07004
location: https://synbiohub.org/public/igem/BBa_J07004/annotation1523107/range1523107/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_J07004/annotation1523107/range1523107/1
type: Range
persistent identity: range1523107
display id: range1523107
version: 1
top level: BBa_J07004
direction: direction/forward
start: 1423
end: 1434
orientation: inline

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation1523098
display id: annotation1523098
version: 1
top level: BBa_J07004
location: https://synbiohub.org/public/igem/BBa_J07004/annotation1523098/range1523098/1
component: BBa_R0011

https://synbiohub.org/public/igem/BBa_J07004/annotation1523098/range1523098/1
type: Range
persistent identity: range1523098
display id: range1523098
version: 1
top level: BBa_J07004
direction: direction/forward
start: 1360
end: 1413
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1523123
display id: annotation1523123
version: 1
top level: BBa_J07004
location: https://synbiohub.org/public/igem/BBa_J07004/annotation1523123/range1523123/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_J07004/annotation1523123/range1523123/1
type: Range
persistent identity: range1523123
display id: range1523123
version: 1
top level: BBa_J07004
direction: direction/forward
start: 2134
end: 2213
orientation: inline

BBa_Z0251
type: SequenceAnnotation
persistent identity: annotation1523052
display id: annotation1523052
version: 1
top level: BBa_J07004
location: https://synbiohub.org/public/igem/BBa_J07004/annotation1523052/range1523052/1
component: BBa_Z0251

https://synbiohub.org/public/igem/BBa_J07004/annotation1523052/range1523052/1
type: Range
persistent identity: range1523052
display id: range1523052
version: 1
top level: BBa_J07004
direction: direction/forward
start: 1
end: 35
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1523148
display id: annotation1523148
version: 1
top level: BBa_J07004
location: https://synbiohub.org/public/igem/BBa_J07004/annotation1523148/range1523148/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_J07004/annotation1523148/range1523148/1
type: Range
persistent identity: range1523148
display id: range1523148
version: 1
top level: BBa_J07004
direction: direction/forward
start: 2271
end: 2324
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1523084
display id: annotation1523084
version: 1
top level: BBa_J07004
location: https://synbiohub.org/public/igem/BBa_J07004/annotation1523084/range1523084/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_J07004/annotation1523084/range1523084/1
type: Range
persistent identity: range1523084
display id: range1523084
version: 1
top level: BBa_J07004
direction: direction/forward
start: 1311
end: 1351
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1523057
display id: annotation1523057
version: 1
top level: BBa_J07004
location: https://synbiohub.org/public/igem/BBa_J07004/annotation1523057/range1523057/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_J07004/annotation1523057/range1523057/1
type: Range
persistent identity: range1523057
display id: range1523057
version: 1
top level: BBa_J07004
direction: direction/forward
start: 44
end: 55
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1523173
display id: annotation1523173
version: 1
top level: BBa_J07004
location: https://synbiohub.org/public/igem/BBa_J07004/annotation1523173/range1523173/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_J07004/annotation1523173/range1523173/1
type: Range
persistent identity: range1523173
display id: range1523173
version: 1
top level: BBa_J07004
direction: direction/forward
start: 3170
end: 3210
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1523133
display id: annotation1523133
version: 1
top level: BBa_J07004
location: https://synbiohub.org/public/igem/BBa_J07004/annotation1523133/range1523133/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_J07004/annotation1523133/range1523133/1
type: Range
persistent identity: range1523133
display id: range1523133
version: 1
top level: BBa_J07004
direction: direction/forward
start: 2222
end: 2262
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1523163
display id: annotation1523163
version: 1
top level: BBa_J07004
location: https://synbiohub.org/public/igem/BBa_J07004/annotation1523163/range1523163/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_J07004/annotation1523163/range1523163/1
type: Range
persistent identity: range1523163
display id: range1523163
version: 1
top level: BBa_J07004
direction: direction/forward
start: 3082
end: 3161
orientation: inline

BBa_E0020
type: SequenceAnnotation
persistent identity: annotation1523158
display id: annotation1523158
version: 1
top level: BBa_J07004
location: https://synbiohub.org/public/igem/BBa_J07004/annotation1523158/range1523158/1
component: BBa_E0020

https://synbiohub.org/public/igem/BBa_J07004/annotation1523158/range1523158/1
type: Range
persistent identity: range1523158
display id: range1523158
version: 1
top level: BBa_J07004
direction: direction/forward
start: 2351
end: 3073
orientation: inline

BBa_C0040
type: SequenceAnnotation
persistent identity: annotation1523117
display id: annotation1523117
version: 1
top level: BBa_J07004
location: https://synbiohub.org/public/igem/BBa_J07004/annotation1523117/range1523117/1
component: BBa_C0040

https://synbiohub.org/public/igem/BBa_J07004/annotation1523117/range1523117/1
type: Range
persistent identity: range1523117
display id: range1523117
version: 1
top level: BBa_J07004
direction: direction/forward
start: 1441
end: 2100
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1523156
display id: annotation1523156
version: 1
top level: BBa_J07004
location: https://synbiohub.org/public/igem/BBa_J07004/annotation1523156/range1523156/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_J07004/annotation1523156/range1523156/1
type: Range
persistent identity: range1523156
display id: range1523156
version: 1
top level: BBa_J07004
direction: direction/forward
start: 2333
end: 2344
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1523074
display id: annotation1523074
version: 1
top level: BBa_J07004
location: https://synbiohub.org/public/igem/BBa_J07004/annotation1523074/range1523074/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_J07004/annotation1523074/range1523074/1
type: Range
persistent identity: range1523074
display id: range1523074
version: 1
top level: BBa_J07004
direction: direction/forward
start: 1223
end: 1302
orientation: inline

spacer
type: ComponentDefinition
persistent identity: BBa B0040
display id: BBa_B0040
version: 1
was generated by: iGEM to SBOL conversion
description: Spacer.1 (generic)
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Randomly generated and optimized for several parameters (see Design notes).
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Generic spacer for ensuring a 70 bp distance between the end of the suffix of the BioBrick part containing the double terminator and the prefix of the BioBrick part containing the promoter of the new gene. Please, use the AlignX function of Vector NT to check for homology with the components in your plasmid before using this spacer.
discontinued: false
dominant: false
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: The size of the spacer was choosed to meet the minimum length of a sequence that can be queried using the BLAST search engine. However, subsequences of it can be used to design shorter spacers. The sequence was selected from many more sequences randomly generated using the Random DNA Generator engine; the GC% parameter used as input was 50%. The sequences were selected based on the following constraints listed in their order of importance: the absence of any putative promoter regions, a low degree of homology with the Elowitz plasmid (whose components are widely used in our designs), no homology with other E.coli sequences as shown by BLASTN search results and the presence of a number of TAA stop codons. The second constraint was the most stringent leading to the elimination of most sequences. DE made the following changes to the original sequence in order to add stop codons in the -3 frame and more in the +2 frame (note, not all of these stop codons are UAA. Thus, if used in an organism that inserts an amino acid @ UGA or UAG the obvious will occur): T->A @ 85 T->A @ 42 C->T @ 79 A->T @ 64 A->T @ 31 T->A @ 34 C->A @ 37 Also, note that the above changes further reduce (the already very weak) homology to current NCBI-stored sequences. In the process of selecting the best sequence it appeared that a good alternative sequence for a spacer would be: AGGTTCTGATATGTAACTGTGCCCAATGTCGTTAGTGACGCATACCTCTTAAGAGGCCACTGTCCTAACA. The sequence contains no putative promoters and shows moderate homology with the 5' end of the Ampicillin resistance gene. However a strong promoter sequence starts 12 bp downstream of this sequence, and therefore the sequence presented above was preferred. The sequence is compatible (does not show significant homology) with the components in the Elowitz repressilator plasmid.
star: true
creator: Vinay S. Mahajan, Brian Chow, Peter Carr
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: Spacer-1
sequence annotation: BBa_B0040
sequence: https://synbiohub.org/public/igem/BBa_B0040_sequence/1

Spacer-1
type: SequenceAnnotation
persistent identity: annotation1721
display id: annotation1721
version: 1
top level: spacer
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_B0040/annotation1721/range1721/1

https://synbiohub.org/public/igem/BBa_B0040/annotation1721/range1721/1
type: Range
persistent identity: range1721
display id: range1721
version: 1
top level: spacer
direction: direction/forward
start: 1
end: 70
orientation: inline

BBa_B0040
type: SequenceAnnotation
persistent identity: annotation7030
display id: annotation7030
version: 1
top level: spacer
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_B0040/annotation7030/range7030/1

https://synbiohub.org/public/igem/BBa_B0040/annotation7030/range7030/1
type: Range
persistent identity: range7030
display id: range7030
version: 1
top level: spacer
direction: direction/forward
start: 1
end: 70
orientation: inline

tetR
type: ComponentDefinition
persistent identity: BBa C0040
display id: BBa_C0040
version: 1
was generated by: iGEM to SBOL conversion
description: tetracycline repressor from transposon Tn10 (+LVA)
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:23Z
mutable provenance: Elowitz, M. B. Transport, Assembly, and Dynamics in Systems of Interacting Proteins. Thesis, Princeton Univ., Princeton (1999)
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Coding region for the TetR protein without the Ribosome Binding Site. Modified with an LVA tail for rapid degradation of the protein and faster fall time for the emission. TetR binds to the pTet regulator (BBa_R0040). aTc (anhydrotetracycline) binds to TetR and inhibits its operation.
discontinued: false
dominant: true
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: References (unparsed) here: Elowitz, M. B. Transport, Assembly, and Dynamics in Systems of Interacting Proteins. Thesis, Princeton Univ., Princeton (1999). Lutz R, Bujard H., Independent and tight regulation of transcriptional units in Escherichia coli via the LacR/O, the TetR/O and AraC/I1-I2 regulatory elements. Nucleic Acids Res. 1997 Mar 15;25(6):1203-10. PMID: 9092630 References (unparsed) here: Elowitz, M. B. Transport, Assembly, and Dynamics in Systems of Interacting Proteins. Thesis, Princeton Univ., Princeton (1999). Lutz R, Bujard H., Independent and tight regulation of transcriptional units in Escherichia coli via the LacR/O, the TetR/O and AraC/I1-I2 regulatory elements. Nucleic Acids Res. 1997 Mar 15;25(6):1203-10. PMID: 9092630 BBa_C0040 TetR Protein is based on the TetR sequence from Elowitz's repressilator. It has been modified to include a rapid degradation LVA tail, and includes the BioBrick standard assembly head and tail restriction sites. The RBS has been removed. The stop codon has been changed from TAA to a double stop codon TAATAA.
star: true
creator: June Rhee, Connie Tao, Ty Thomson, Louis Waldman.
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Help:Barcodes
sequence annotation: SsrA
sequence annotation: tetR
sequence: https://synbiohub.org/public/igem/BBa_C0040_sequence/1

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213989
display id: annotation2213989
version: 1
top level: tetR
role: SO:0000807
role: feature/barcode
location: https://synbiohub.org/public/igem/BBa_C0040/annotation2213989/range2213989/1

https://synbiohub.org/public/igem/BBa_C0040/annotation2213989/range2213989/1
type: Range
persistent identity: range2213989
display id: range2213989
version: 1
top level: tetR
direction: direction/forward
start: 661
end: 685
orientation: inline

SsrA
type: SequenceAnnotation
persistent identity: annotation23330
display id: annotation23330
version: 1
top level: tetR
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_C0040/annotation23330/range23330/1

https://synbiohub.org/public/igem/BBa_C0040/annotation23330/range23330/1
type: Range
persistent identity: range23330
display id: range23330
version: 1
top level: tetR
direction: direction/forward
start: 621
end: 654
orientation: inline

tetR
type: SequenceAnnotation
persistent identity: annotation23329
display id: annotation23329
version: 1
top level: tetR
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_C0040/annotation23329/range23329/1

https://synbiohub.org/public/igem/BBa_C0040/annotation23329/range23329/1
type: Range
persistent identity: range23329
display id: range23329
version: 1
top level: tetR
direction: direction/forward
start: 4
end: 620
orientation: inline

P22 attB
type: ComponentDefinition
persistent identity: BBa I11032
display id: BBa_I11032
version: 1
was generated by: iGEM to SBOL conversion
description: P22 ''attB'', reverse complement
created: 2004-09-02T11:00:00Z
modified: 2015-08-31T04:07:30Z
mutable provenance: gi|49175990:262118-262144 rev comp
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Reverse Complement of P22 attB site (B'-O-B)
discontinued: false
dominant: false
experience: experience/None
group u list: _2_
m user id: 0
owner id: 102
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: mschomp
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_I11032_sequence/1

Lambda att
type: ComponentDefinition
persistent identity: BBa I11022
display id: BBa_I11022
version: 1
was generated by: iGEM to SBOL conversion
description: Lambda attB, reverse complement
created: 2004-09-02T11:00:00Z
modified: 2015-08-31T04:07:30Z
owned by: james
owned by: myers
mutable description: Lambda phage attB site, reverse complement (B'-O-B)
discontinued: false
dominant: false
experience: experience/None
group u list: _2_
m user id: 0
owner id: 102
owning group id: 7
sample status: It's complicated
status: status/Planning
bookmark: false
star: false
creator: mschomp
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: lambda attB rev comp (B'-O-B)
sequence: https://synbiohub.org/public/igem/BBa_I11022_sequence/1

lambda attB rev comp (B'-O-B)
type: SequenceAnnotation
persistent identity: annotation1064122
display id: annotation1064122
version: 1
top level: Lambda att
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I11022/annotation1064122/range1064122/1

https://synbiohub.org/public/igem/BBa_I11022/annotation1064122/range1064122/1
type: Range
persistent identity: range1064122
display id: range1064122
version: 1
top level: Lambda att
direction: direction/forward
start: 1
end: 25
orientation: inline

BBa_I11052
type: ComponentDefinition
persistent identity: BBa I11052
display id: BBa_I11052
version: 1
was generated by: iGEM to SBOL conversion
description: P21 attB rev comp
created: 2004-09-06T11:00:00Z
modified: 2015-08-31T04:07:30Z
mutable provenance: J Bacteriol, 1997; Sep:179(18):5705-5711
owned by: james
owned by: myers
mutable description: Reverse complement of P21 attB attachment site
discontinued: false
dominant: false
experience: experience/None
group u list: _2_
m user id: 0
owner id: 102
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: bu
type: DnaRegion
role: partType/Other
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_I11052_sequence/1

BBa_J07001
type: ComponentDefinition
persistent identity: BBa J07001
display id: BBa_J07001
version: 1
was generated by: iGEM to SBOL conversion
description: abandoned test construct
created: 2005-06-15T11:00:00Z
modified: 2015-08-31T04:08:19Z
owned by: james
owned by: myers
discontinued: false
dominant: false
experience: experience/None
group u list: _21_
m user id: 0
owner id: 350
owning group id: 21
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Jenny
type: DnaRegion
role: partType/Other
role: SO:0000110
component: BBa_R0053
component: BBa_B0010
component: BBa_R0011
component: BBa_B0012
component: BBa_B0034
component: BBa_C0012
component: BBa_E0030
component: BBa_C0040
component: BBa_R0040
sequence annotation: BBa_R0040
sequence annotation: BBa_B0012
sequence annotation: BBa_B0010
sequence annotation: BBa_R0053
sequence annotation: BBa_C0012
sequence annotation: BBa_C0040
sequence annotation: BBa_E0030
sequence annotation: BBa_R0011
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_J07001_sequence/1

BBa_R0053
type: Component
persistent identity: component1521702
display id: component1521702
version: 1
top level: BBa_J07001
access: public
definition: cII p22

BBa_B0010
type: Component
persistent identity: component1521729
display id: component1521729
version: 1
top level: BBa_J07001
access: public
definition: BBa_B0010

BBa_R0011
type: Component
persistent identity: component1521753
display id: component1521753
version: 1
top level: BBa_J07001
access: public
definition: lacI+pL

BBa_B0012
type: Component
persistent identity: component1521788
display id: component1521788
version: 1
top level: BBa_J07001
access: public
definition: BBa_B0012

BBa_B0034
type: Component
persistent identity: component1521712
display id: component1521712
version: 1
top level: BBa_J07001
access: public
definition: BBa_B0034

BBa_B0012
type: Component
persistent identity: component1521739
display id: component1521739
version: 1
top level: BBa_J07001
access: public
definition: BBa_B0012

BBa_C0012
type: Component
persistent identity: component1521722
display id: component1521722
version: 1
top level: BBa_J07001
access: public
definition: lacI

BBa_E0030
type: Component
persistent identity: component1521813
display id: component1521813
version: 1
top level: BBa_J07001
access: public
definition: eyfp

BBa_B0010
type: Component
persistent identity: component1521778
display id: component1521778
version: 1
top level: BBa_J07001
access: public
definition: BBa_B0010

BBa_C0040
type: Component
persistent identity: component1521772
display id: component1521772
version: 1
top level: BBa_J07001
access: public
definition: tetR

BBa_R0040
type: Component
persistent identity: component1521803
display id: component1521803
version: 1
top level: BBa_J07001
access: public
definition: p(tetR)

BBa_B0012
type: Component
persistent identity: component1521828
display id: component1521828
version: 1
top level: BBa_J07001
access: public
definition: BBa_B0012

BBa_B0010
type: Component
persistent identity: component1521818
display id: component1521818
version: 1
top level: BBa_J07001
access: public
definition: BBa_B0010

BBa_B0034
type: Component
persistent identity: component1521811
display id: component1521811
version: 1
top level: BBa_J07001
access: public
definition: BBa_B0034

BBa_B0034
type: Component
persistent identity: component1521762
display id: component1521762
version: 1
top level: BBa_J07001
access: public
definition: BBa_B0034

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1521803
display id: annotation1521803
version: 1
top level: BBa_J07001
location: https://synbiohub.org/public/igem/BBa_J07001/annotation1521803/range1521803/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_J07001/annotation1521803/range1521803/1
type: Range
persistent identity: range1521803
display id: range1521803
version: 1
top level: BBa_J07001
direction: direction/forward
start: 2290
end: 2343
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1521828
display id: annotation1521828
version: 1
top level: BBa_J07001
location: https://synbiohub.org/public/igem/BBa_J07001/annotation1521828/range1521828/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_J07001/annotation1521828/range1521828/1
type: Range
persistent identity: range1521828
display id: range1521828
version: 1
top level: BBa_J07001
direction: direction/forward
start: 3189
end: 3229
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1521729
display id: annotation1521729
version: 1
top level: BBa_J07001
location: https://synbiohub.org/public/igem/BBa_J07001/annotation1521729/range1521729/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_J07001/annotation1521729/range1521729/1
type: Range
persistent identity: range1521729
display id: range1521729
version: 1
top level: BBa_J07001
direction: direction/forward
start: 1242
end: 1321
orientation: inline

BBa_R0053
type: SequenceAnnotation
persistent identity: annotation1521702
display id: annotation1521702
version: 1
top level: BBa_J07001
location: https://synbiohub.org/public/igem/BBa_J07001/annotation1521702/range1521702/1
component: BBa_R0053

https://synbiohub.org/public/igem/BBa_J07001/annotation1521702/range1521702/1
type: Range
persistent identity: range1521702
display id: range1521702
version: 1
top level: BBa_J07001
direction: direction/forward
start: 1
end: 54
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1521818
display id: annotation1521818
version: 1
top level: BBa_J07001
location: https://synbiohub.org/public/igem/BBa_J07001/annotation1521818/range1521818/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_J07001/annotation1521818/range1521818/1
type: Range
persistent identity: range1521818
display id: range1521818
version: 1
top level: BBa_J07001
direction: direction/forward
start: 3101
end: 3180
orientation: inline

BBa_C0012
type: SequenceAnnotation
persistent identity: annotation1521722
display id: annotation1521722
version: 1
top level: BBa_J07001
location: https://synbiohub.org/public/igem/BBa_J07001/annotation1521722/range1521722/1
component: BBa_C0012

https://synbiohub.org/public/igem/BBa_J07001/annotation1521722/range1521722/1
type: Range
persistent identity: range1521722
display id: range1521722
version: 1
top level: BBa_J07001
direction: direction/forward
start: 81
end: 1208
orientation: inline

BBa_C0040
type: SequenceAnnotation
persistent identity: annotation1521772
display id: annotation1521772
version: 1
top level: BBa_J07001
location: https://synbiohub.org/public/igem/BBa_J07001/annotation1521772/range1521772/1
component: BBa_C0040

https://synbiohub.org/public/igem/BBa_J07001/annotation1521772/range1521772/1
type: Range
persistent identity: range1521772
display id: range1521772
version: 1
top level: BBa_J07001
direction: direction/forward
start: 1460
end: 2119
orientation: inline

BBa_E0030
type: SequenceAnnotation
persistent identity: annotation1521813
display id: annotation1521813
version: 1
top level: BBa_J07001
location: https://synbiohub.org/public/igem/BBa_J07001/annotation1521813/range1521813/1
component: BBa_E0030

https://synbiohub.org/public/igem/BBa_J07001/annotation1521813/range1521813/1
type: Range
persistent identity: range1521813
display id: range1521813
version: 1
top level: BBa_J07001
direction: direction/forward
start: 2370
end: 3092
orientation: inline

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation1521753
display id: annotation1521753
version: 1
top level: BBa_J07001
location: https://synbiohub.org/public/igem/BBa_J07001/annotation1521753/range1521753/1
component: BBa_R0011

https://synbiohub.org/public/igem/BBa_J07001/annotation1521753/range1521753/1
type: Range
persistent identity: range1521753
display id: range1521753
version: 1
top level: BBa_J07001
direction: direction/forward
start: 1379
end: 1432
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1521811
display id: annotation1521811
version: 1
top level: BBa_J07001
location: https://synbiohub.org/public/igem/BBa_J07001/annotation1521811/range1521811/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_J07001/annotation1521811/range1521811/1
type: Range
persistent identity: range1521811
display id: range1521811
version: 1
top level: BBa_J07001
direction: direction/forward
start: 2352
end: 2363
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1521739
display id: annotation1521739
version: 1
top level: BBa_J07001
location: https://synbiohub.org/public/igem/BBa_J07001/annotation1521739/range1521739/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_J07001/annotation1521739/range1521739/1
type: Range
persistent identity: range1521739
display id: range1521739
version: 1
top level: BBa_J07001
direction: direction/forward
start: 1330
end: 1370
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1521788
display id: annotation1521788
version: 1
top level: BBa_J07001
location: https://synbiohub.org/public/igem/BBa_J07001/annotation1521788/range1521788/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_J07001/annotation1521788/range1521788/1
type: Range
persistent identity: range1521788
display id: range1521788
version: 1
top level: BBa_J07001
direction: direction/forward
start: 2241
end: 2281
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1521778
display id: annotation1521778
version: 1
top level: BBa_J07001
location: https://synbiohub.org/public/igem/BBa_J07001/annotation1521778/range1521778/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_J07001/annotation1521778/range1521778/1
type: Range
persistent identity: range1521778
display id: range1521778
version: 1
top level: BBa_J07001
direction: direction/forward
start: 2153
end: 2232
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1521762
display id: annotation1521762
version: 1
top level: BBa_J07001
location: https://synbiohub.org/public/igem/BBa_J07001/annotation1521762/range1521762/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_J07001/annotation1521762/range1521762/1
type: Range
persistent identity: range1521762
display id: range1521762
version: 1
top level: BBa_J07001
direction: direction/forward
start: 1442
end: 1453
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1521712
display id: annotation1521712
version: 1
top level: BBa_J07001
location: https://synbiohub.org/public/igem/BBa_J07001/annotation1521712/range1521712/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_J07001/annotation1521712/range1521712/1
type: Range
persistent identity: range1521712
display id: range1521712
version: 1
top level: BBa_J07001
direction: direction/forward
start: 63
end: 74
orientation: inline

Homol.
type: ComponentDefinition
persistent identity: BBa I13851
display id: BBa_I13851
version: 1
was generated by: iGEM to SBOL conversion
description: E coli Upstream Homologous Recombination Sequence
created: 2004-07-22T11:00:00Z
modified: 2015-08-31T04:07:36Z
owned by: james
owned by: myers
mutable description: This is the upstream sequence of the homologous recombination site for the E coli chromosome provided by Chris Farrell. The recombination site is in between the ybhH and ybhD genes on the E coli chromosome.
discontinued: false
dominant: false
experience: experience/None
group u list: _6_
m user id: 0
owner id: 159
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: robertb
type: DnaRegion
role: partType/Other
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_I13851_sequence/1

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: stop
sequence annotation: T7 TE
sequence annotation: polya
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

BBa_Y1010
type: ComponentDefinition
persistent identity: BBa Y1010
display id: BBa_Y1010
version: 1
was generated by: iGEM to SBOL conversion
description: Gal1 Promoter
created: 2005-06-22T11:00:00Z
modified: 2015-05-08T01:14:54Z
owned by: james
owned by: myers
mutable description: Promoter
discontinued: false
dominant: false
experience: experience/None
group u list: _11_
m user id: 0
owner id: 351
owning group id: 43
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Samantha Sutton
type: DnaRegion
role: partType/Other
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_Y1010_sequence/1

BBa_Y1003
type: ComponentDefinition
persistent identity: BBa Y1003
display id: BBa_Y1003
version: 1
was generated by: iGEM to SBOL conversion
description: Kanamyacin Resistance Gene
created: 2005-06-22T11:00:00Z
modified: 2015-05-08T01:14:54Z
owned by: james
owned by: myers
mutable description: Has its own promoter and terminator
discontinued: false
dominant: false
experience: experience/None
group u list: _11_
m user id: 0
owner id: 351
owning group id: 43
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Samantha Sutton
type: DnaRegion
role: partType/Other
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_Y1003_sequence/1

BBa_Y1005
type: ComponentDefinition
persistent identity: BBa Y1005
display id: BBa_Y1005
version: 1
was generated by: iGEM to SBOL conversion
description: 2u
created: 2005-06-22T11:00:00Z
modified: 2015-05-08T01:14:54Z
owned by: james
owned by: myers
mutable description: Yeast origin
discontinued: false
dominant: false
experience: experience/None
group u list: _11_
m user id: 0
owner id: 351
owning group id: 43
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Samantha Sutton
type: DnaRegion
role: partType/Other
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_Y1005_sequence/1

BBa_I11053
type: ComponentDefinition
persistent identity: BBa I11053
display id: BBa_I11053
version: 1
was generated by: iGEM to SBOL conversion
description: P21 attP
created: 2004-09-06T11:00:00Z
modified: 2015-08-31T04:07:30Z
mutable provenance: gi|215448:2092-2509
owned by: james
owned by: myers
mutable description: P21 attP attachment site
discontinued: false
dominant: false
experience: experience/None
group u list: _2_
m user id: 0
owner id: 102
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: bu
type: DnaRegion
role: partType/Other
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_I11053_sequence/1

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: stem_loop
sequence annotation: BBa_B0010
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

trkD
type: ComponentDefinition
persistent identity: BBa K629005
display id: BBa_K629005
version: 1
was generated by: iGEM to SBOL conversion
description: trkD, a functional Kup (formerly TrkD) system took up Cs+ with a moderate rate and affinity
created: 2011-10-03T11:00:00Z
modified: 2015-05-08T01:12:56Z
mutable provenance: ATGAGCACTGATAATAAGCAATCATTGCCCGCGATTACCCTCGCGGCGATTGGAGTTGTCTACGGCGATATTGGTACCAGCCCGTTATATACACTTCGTGAATGTTTGTCCGGCCAGTTTGGTTTTGGCGTTGAACGCGATGCCGTGTTTGGCTTTTTATCGCTGATCTTCTGGCTGCTAATCTTTGTGGTTTCCATTAAATATCTCACCTTCGTGATGCGGGCAGATAACGCCGGTGAGGGGGGGATCCTGACGTTGATGTCGCTTGCCGGGCGTAATACGTCGGCGCGAACCACATCAATGCTGGTGATTATGGGGCTAATCGGCGGCAGCTTTTTCTATGGTGAAGTCGTCATAACACCCGCTATTTCGGTGATGTCAGCCATTGAAGGTCTGGAAATCGTCGCCCCGCAGCTGGATACCTGGATAGTTCCCCTCTCAATTATCGTTCTCACTTTATTATTTATGATTCAAAAGCATGGCACGGCTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTGGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGTGCTGCATGCGCTGAATCCGATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGCTGTCGATTACGGGGGTCGAGGCGCTGTATGCTGATATGGGACACTTTGGTAAGTTCCCTATTCGTCTGGCGTGGTTCACTGTCGTATTACCTTCCTTAACTCTTAATTACTTCGGCCAGGGAGCGCTGTTGTTAAAGAACCCGGAAGCGATTAAGAACCCGTTCTTCCTTTTGGCACCGGACTGGGCGCTGATCCCGCTGCTGATCATCGCCGCACTGGCGACGGTAATCGCCTCGCAGGCGGTTATCTCTGGCGTCTTTTCATTGACGCGTCAGGCGGTACGTCTGGGATATTTGTCGCCGATGCGCATTATTCACACCTCCGAAATGGAGTCAGGGCAAATCTATATTCCGTTTGTGAACTGGATGCTCTATGTCGCGGTCGTGATTGTGATTGTCAGCTTTGAGCACTCCAGCAACCTGGCGGCGGCGTACGGGATTGCGGTGACCGGAACCATGGTGCTGACGTCTATTCTCTCGACTACCGTGGCACGTCAGAACTGGCACTGGAATAAGTATTTTGTTGCGCTGATCCTGATTGCTTTCCTTTGTGTCGATATTCCATTGTTCACCGCTAACCTCGATAAACTGCTCTCCGGCGGCTGGTTGCCATTGAGCCTCGGTACTGTGATGTTTATCGTGATGACCACCTGGAAGAGCGAGCGTTTCCGCTTGCTGCGGCGGATGCATGAACATGGTAACTCTCTGGAAGCGATGATTGCTTCGCTGGAGAAATCACCGCCTGTTCGCGTGCCCGGGACCGCGGTGTATATGTCGCGTGCAATCAACGTCATTCCCTTTGCGCTGATGCATAACCTTAAACATAACAAGGTATTGCATGAGCGGGTGATTCTGTTAACTCTGCGCACCGAAGACGCTCCATATGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAA
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Escherichia coli cells which contain a functional Kup (formerly TrkD) system took up Cs' with a moderate rate and affinity. Kup is a separate K+ uptake system with relatively little discrimination in the transport of the cations K+, Rb+, and Cs'. Regardless of the presence or absence of Kup, K+-replete cells took up Cs'primarily by a very low affinity mode, proportional to the ratio of the Cs' and K+ concentrations in the medium. In our project, trkD is used to collect Cs-137 when recN is activated by radiation and start the transcription of trkD,in order to absorb this kind of radioactive source like Cs-137
discontinued: false
dominant: false
experience: experience/Works
group u list: _801_
m user id: 0
owner id: 9022
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable notes: none
star: false
creator: Zilong WANG, Yi ZHENG
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: TrkD
sequence annotation: start
sequence annotation: fwd primer
sequence annotation: terminator
sequence annotation: rev primer
sequence: https://synbiohub.org/public/igem/BBa_K629005_sequence/1

TrkD
type: SequenceAnnotation
persistent identity: annotation2157756
display id: annotation2157756
version: 1
top level: trkD
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K629005/annotation2157756/range2157756/1

https://synbiohub.org/public/igem/BBa_K629005/annotation2157756/range2157756/1
type: Range
persistent identity: range2157756
display id: range2157756
version: 1
top level: trkD
direction: direction/forward
start: 1
end: 1869
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2157754
display id: annotation2157754
version: 1
top level: trkD
role: feature/start
role: SO:0000318
location: https://synbiohub.org/public/igem/BBa_K629005/annotation2157754/range2157754/1

https://synbiohub.org/public/igem/BBa_K629005/annotation2157754/range2157754/1
type: Range
persistent identity: range2157754
display id: range2157754
version: 1
top level: trkD
direction: direction/forward
start: 1
end: 3
orientation: inline

fwd primer
type: SequenceAnnotation
persistent identity: annotation2157755
display id: annotation2157755
version: 1
top level: trkD
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K629005/annotation2157755/range2157755/1

https://synbiohub.org/public/igem/BBa_K629005/annotation2157755/range2157755/1
type: Range
persistent identity: range2157755
display id: range2157755
version: 1
top level: trkD
direction: direction/forward
start: 1
end: 21
orientation: inline

terminator
type: SequenceAnnotation
persistent identity: annotation2157758
display id: annotation2157758
version: 1
top level: trkD
role: feature/stop
role: SO:0000319
location: https://synbiohub.org/public/igem/BBa_K629005/annotation2157758/range2157758/1

https://synbiohub.org/public/igem/BBa_K629005/annotation2157758/range2157758/1
type: Range
persistent identity: range2157758
display id: range2157758
version: 1
top level: trkD
direction: direction/forward
start: 1867
end: 1869
orientation: inline

rev primer
type: SequenceAnnotation
persistent identity: annotation2157757
display id: annotation2157757
version: 1
top level: trkD
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K629005/annotation2157757/range2157757/1

https://synbiohub.org/public/igem/BBa_K629005/annotation2157757/range2157757/1
type: Range
persistent identity: range2157757
display id: range2157757
version: 1
top level: trkD
direction: direction/reverse
start: 1849
end: 1869
orientation: inline

ecfp
type: ComponentDefinition
persistent identity: BBa E0020
display id: BBa_E0020
version: 1
was generated by: iGEM to SBOL conversion
description: engineered cyan fluorescent protein derived from A. victoria GFP
created: 2004-03-02T12:00:00Z
modified: 2015-08-31T04:07:25Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: -- No description --
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Caitlin Conboy and Jennifer Braff
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_E0020_sequence/1

P22 attP
type: ComponentDefinition
persistent identity: BBa I11033
display id: BBa_I11033
version: 1
was generated by: iGEM to SBOL conversion
description: P22 ''attP''
created: 2004-09-02T11:00:00Z
modified: 2015-08-31T04:07:30Z
mutable provenance: gi|51236723:21158-21417
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: P22 phage attP site (P-O-P')
discontinued: false
dominant: false
experience: experience/None
group u list: _2_
m user id: 0
owner id: 102
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: 2004 Boston University iGEM team
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_I11033_sequence/1

BBa_Y1014
type: ComponentDefinition
persistent identity: BBa Y1014
display id: BBa_Y1014
version: 1
was generated by: iGEM to SBOL conversion
description: GPD Promoter
created: 2005-06-22T11:00:00Z
modified: 2015-05-08T01:14:54Z
owned by: james
owned by: myers
mutable description: Promoter
discontinued: false
dominant: false
experience: experience/None
group u list: _11_
m user id: 0
owner id: 351
owning group id: 43
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Samantha Sutton
type: DnaRegion
role: partType/Other
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_Y1014_sequence/1

BBa_J07031
type: ComponentDefinition
persistent identity: BBa J07031
display id: BBa_J07031
version: 1
was generated by: iGEM to SBOL conversion
description: Antirabbit IgG scFV: no Pst1 or Not1 sites
created: 2005-07-17T11:00:00Z
modified: 2015-08-31T04:08:19Z
mutable provenance: Shen, et al. "Single-chain Fragment Variable Antibody Piezoimmunosensors" Analytical Chemistry, Vol. 77, No. 3, February 1, 2005.
owned by: james
owned by: myers
mutable description: Single chain fragment variable produced from the genetic information of an A10B hybridoma cell line. This scFV binds to the constant heavy chain 1 domain of rabbit IgG.
discontinued: false
dominant: false
experience: experience/None
group u list: _21_
m user id: 0
owner id: 355
owning group id: 21
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: maxiney
type: DnaRegion
role: partType/Other
role: SO:0000110
sequence annotation: Variable heavy chain region
sequence annotation: Stop codon
sequence annotation: Linker sequence
sequence annotation: XbaI restriction site
sequence annotation: Variable light chain region
sequence annotation: SpeI restriction site
sequence: https://synbiohub.org/public/igem/BBa_J07031_sequence/1

Variable heavy chain region
type: SequenceAnnotation
persistent identity: annotation1586177
display id: annotation1586177
version: 1
top level: BBa_J07031
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J07031/annotation1586177/range1586177/1

https://synbiohub.org/public/igem/BBa_J07031/annotation1586177/range1586177/1
type: Range
persistent identity: range1586177
display id: range1586177
version: 1
top level: BBa_J07031
direction: direction/forward
start: 7
end: 369
orientation: inline

Stop codon
type: SequenceAnnotation
persistent identity: annotation1586664
display id: annotation1586664
version: 1
top level: BBa_J07031
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J07031/annotation1586664/range1586664/1

https://synbiohub.org/public/igem/BBa_J07031/annotation1586664/range1586664/1
type: Range
persistent identity: range1586664
display id: range1586664
version: 1
top level: BBa_J07031
direction: direction/forward
start: 745
end: 750
orientation: inline

Linker sequence
type: SequenceAnnotation
persistent identity: annotation1586178
display id: annotation1586178
version: 1
top level: BBa_J07031
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J07031/annotation1586178/range1586178/1

https://synbiohub.org/public/igem/BBa_J07031/annotation1586178/range1586178/1
type: Range
persistent identity: range1586178
display id: range1586178
version: 1
top level: BBa_J07031
direction: direction/forward
start: 370
end: 414
orientation: inline

XbaI restriction site
type: SequenceAnnotation
persistent identity: annotation1586175
display id: annotation1586175
version: 1
top level: BBa_J07031
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J07031/annotation1586175/range1586175/1

https://synbiohub.org/public/igem/BBa_J07031/annotation1586175/range1586175/1
type: Range
persistent identity: range1586175
display id: range1586175
version: 1
top level: BBa_J07031
direction: direction/forward
start: 1
end: 6
orientation: inline

Variable light chain region
type: SequenceAnnotation
persistent identity: annotation1586179
display id: annotation1586179
version: 1
top level: BBa_J07031
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J07031/annotation1586179/range1586179/1

https://synbiohub.org/public/igem/BBa_J07031/annotation1586179/range1586179/1
type: Range
persistent identity: range1586179
display id: range1586179
version: 1
top level: BBa_J07031
direction: direction/forward
start: 415
end: 744
orientation: inline

SpeI restriction site
type: SequenceAnnotation
persistent identity: annotation1586180
display id: annotation1586180
version: 1
top level: BBa_J07031
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J07031/annotation1586180/range1586180/1

https://synbiohub.org/public/igem/BBa_J07031/annotation1586180/range1586180/1
type: Range
persistent identity: range1586180
display id: range1586180
version: 1
top level: BBa_J07031
direction: direction/forward
start: 751
end: 756
orientation: inline

BBa_Y1012
type: ComponentDefinition
persistent identity: BBa Y1012
display id: BBa_Y1012
version: 1
was generated by: iGEM to SBOL conversion
description: Bmh2 Promoter
created: 2005-06-22T11:00:00Z
modified: 2015-05-08T01:14:54Z
owned by: james
owned by: myers
mutable description: Promoter
discontinued: false
dominant: false
experience: experience/None
group u list: _11_
m user id: 0
owner id: 351
owning group id: 43
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Samantha Sutton
type: DnaRegion
role: partType/Other
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_Y1012_sequence/1

lacI
type: ComponentDefinition
persistent identity: BBa C0012
display id: BBa_C0012
version: 1
was generated by: iGEM to SBOL conversion
description: lacI repressor from E. coli (+LVA)
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:23Z
mutable provenance: represillator of Elowitz and Leibler (2000)
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Coding region for the LacI protein with an LVA degradation tail and without an RBS. LacI binds to the pLac regulator <bb_part>BBa_R0010</bb_part> and PLlac01 hybrid regulator <bb_part>BBa_R0011</bb_part> and inhibits transcription. IPTG (Isopropylthiogalactoside) binds to LacI and inhibits its operation, therefore promoting transcription. A rapid degredation tail (LVA) has been added to improve the High to Low performance of this part.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: References (unparsed) here: Elowitz, M.B., Leibler, S. A synthetic oscillatory network of transcriptional regulators. Nature 403, 335-338 (2000). [ELOW00] References (unparsed) here: Elowitz, M.B., Leibler, S. A synthetic oscillatory network of transcriptional regulators. Nature 403, 335-338 (2000). [ELOW00] Sequence taken from the repressilator of Elowitz and Leibler (2000). The obtained sequence was compared to the wild-type sequence for LacI obtained through a database search. The sequence had been modified from the wild-type in that wild-type GTG start was changed to an ATG start (note, actual ORF in E.coli has several GTG starts it would seem). The LVA tag has been added for quicker degradation. Incompatible with systems containing LacI, lactose, or IPTG.
star: true
creator: Grace Kenney, Daniel Shen, Neelaksh Varshney, Samantha Sutton
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Help:Barcodes
sequence annotation: lacI-LVA
sequence annotation: LVA
sequence annotation: BBa_C0012
sequence: https://synbiohub.org/public/igem/BBa_C0012_sequence/1

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213988
display id: annotation2213988
version: 1
top level: lacI
role: SO:0000807
role: feature/barcode
location: https://synbiohub.org/public/igem/BBa_C0012/annotation2213988/range2213988/1

https://synbiohub.org/public/igem/BBa_C0012/annotation2213988/range2213988/1
type: Range
persistent identity: range2213988
display id: range2213988
version: 1
top level: lacI
direction: direction/forward
start: 1129
end: 1153
orientation: inline

lacI-LVA
type: SequenceAnnotation
persistent identity: annotation1723
display id: annotation1723
version: 1
top level: lacI
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_C0012/annotation1723/range1723/1

https://synbiohub.org/public/igem/BBa_C0012/annotation1723/range1723/1
type: Range
persistent identity: range1723
display id: range1723
version: 1
top level: lacI
direction: direction/forward
start: 1
end: 1128
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation1722
display id: annotation1722
version: 1
top level: lacI
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_C0012/annotation1722/range1722/1

https://synbiohub.org/public/igem/BBa_C0012/annotation1722/range1722/1
type: Range
persistent identity: range1722
display id: range1722
version: 1
top level: lacI
direction: direction/forward
start: 1090
end: 1128
orientation: inline

BBa_C0012
type: SequenceAnnotation
persistent identity: annotation7031
display id: annotation7031
version: 1
top level: lacI
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_C0012/annotation7031/range7031/1

https://synbiohub.org/public/igem/BBa_C0012/annotation7031/range7031/1
type: Range
persistent identity: range7031
display id: range7031
version: 1
top level: lacI
direction: direction/forward
start: 1
end: 1128
orientation: inline

BBa_I11043
type: ComponentDefinition
persistent identity: BBa I11043
display id: BBa_I11043
version: 1
was generated by: iGEM to SBOL conversion
description: HK022 attP
created: 2004-09-06T11:00:00Z
modified: 2015-08-31T04:07:30Z
mutable provenance: J Bacteriol, 1990 Sep;172(11):6540-6550
owned by: james
owned by: myers
mutable description: HK022 attP attachment site
discontinued: false
dominant: false
experience: experience/None
group u list: _2_
m user id: 0
owner id: 102
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: bu
type: DnaRegion
role: partType/Other
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_I11043_sequence/1

lacI+pL
type: ComponentDefinition
persistent identity: BBa R0011
display id: BBa_R0011
version: 1
was generated by: iGEM to SBOL conversion
description: Promoter (lacI regulated, lambda pL hybrid)
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: represillator of Elowitz and Leibler (2000)
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) The PLlac 0-1 promoter is a hybrid regulatory region consisting of the promoter P(L) of phage lambda with the cI binding sites replaced with lacO1. The hybrid design allows for strong promotion that can nevertheless be tightly repressed by LacI, the Lac inhibitor (i.e. repressor) (<bb_part>BBa_C0010</bb_part>) ([LUTZ97]). The activity of the promoter can be regulated over a >600-fold range by IPTG in E.Coli DH5-alpha-Z1 (same paper reference).
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: hybrid promoter design to create strong promoter that is, at the same time, highly repressible. note that the upstream operator installed in this hybrid is slightly different than the one in the original source (Lutz and Bujard, 1997). the most upstream operator region is slightly truncated in the represillator version, so that both operators in the hybrid are the same sequence. see references for details. also, the sequence has been truncated after the transcriptional start site. LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and increase transcription. This part is incompatible with environments containing lactose or lactose analogs.
star: true
creator: Neelaksh Varshney, Grace Kenney, Daniel Shen, Samantha Sutton
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -35
sequence annotation: -10
sequence annotation: lac O1
sequence annotation: BBa_R0011
sequence: https://synbiohub.org/public/igem/BBa_R0011_sequence/1

-35
type: SequenceAnnotation
persistent identity: annotation2000
display id: annotation2000
version: 1
top level: lacI+pL
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0011/annotation2000/range2000/1

https://synbiohub.org/public/igem/BBa_R0011/annotation2000/range2000/1
type: Range
persistent identity: range2000
display id: range2000
version: 1
top level: lacI+pL
direction: direction/forward
start: 20
end: 25
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2002
display id: annotation2002
version: 1
top level: lacI+pL
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0011/annotation2002/range2002/1

https://synbiohub.org/public/igem/BBa_R0011/annotation2002/range2002/1
type: Range
persistent identity: range2002
display id: range2002
version: 1
top level: lacI+pL
direction: direction/forward
start: 43
end: 48
orientation: inline

lac O1
type: SequenceAnnotation
persistent identity: annotation1999
display id: annotation1999
version: 1
top level: lacI+pL
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_R0011/annotation1999/range1999/1

https://synbiohub.org/public/igem/BBa_R0011/annotation1999/range1999/1
type: Range
persistent identity: range1999
display id: range1999
version: 1
top level: lacI+pL
direction: direction/forward
start: 3
end: 19
orientation: inline

lac O1
type: SequenceAnnotation
persistent identity: annotation2001
display id: annotation2001
version: 1
top level: lacI+pL
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_R0011/annotation2001/range2001/1

https://synbiohub.org/public/igem/BBa_R0011/annotation2001/range2001/1
type: Range
persistent identity: range2001
display id: range2001
version: 1
top level: lacI+pL
direction: direction/forward
start: 26
end: 42
orientation: inline

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation7064
display id: annotation7064
version: 1
top level: lacI+pL
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_R0011/annotation7064/range7064/1

https://synbiohub.org/public/igem/BBa_R0011/annotation7064/range7064/1
type: Range
persistent identity: range7064
display id: range7064
version: 1
top level: lacI+pL
direction: direction/forward
start: 1
end: 54
orientation: inline

cII p22
type: ComponentDefinition
persistent identity: BBa R0053
display id: BBa_R0053
version: 1
was generated by: iGEM to SBOL conversion
description: Promoter (p22 cII regulated)
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: Bacteriophage p22.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: The p22 cII regulatory region sequence is a 97 base-pair sequence with the standard BioBrick prefix and suffix sections on its ends. p22 cII repressor protein, BBa_C0053, binds to it. This segment contains O-R1, O-R2, a fragment of O-R3, the -35 of P-RM, and P-R (-10 and -35 from Tom Knight)
discontinued: false
dominant: false
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Maia Mahoney
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -35
sequence annotation: OR1
sequence annotation: OR3
sequence annotation: -10
sequence annotation: BBa_R0053
sequence annotation: OR2
sequence: https://synbiohub.org/public/igem/BBa_R0053_sequence/1

-35
type: SequenceAnnotation
persistent identity: annotation2038
display id: annotation2038
version: 1
top level: cII p22
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0053/annotation2038/range2038/1

https://synbiohub.org/public/igem/BBa_R0053/annotation2038/range2038/1
type: Range
persistent identity: range2038
display id: range2038
version: 1
top level: cII p22
direction: direction/forward
start: 18
end: 23
orientation: inline

OR1
type: SequenceAnnotation
persistent identity: annotation2037
display id: annotation2037
version: 1
top level: cII p22
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0053/annotation2037/range2037/1

https://synbiohub.org/public/igem/BBa_R0053/annotation2037/range2037/1
type: Range
persistent identity: range2037
display id: range2037
version: 1
top level: cII p22
direction: direction/forward
start: 34
end: 51
orientation: inline

OR3
type: SequenceAnnotation
persistent identity: annotation2035
display id: annotation2035
version: 1
top level: cII p22
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0053/annotation2035/range2035/1

https://synbiohub.org/public/igem/BBa_R0053/annotation2035/range2035/1
type: Range
persistent identity: range2035
display id: range2035
version: 1
top level: cII p22
direction: direction/forward
start: 1
end: 3
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2042
display id: annotation2042
version: 1
top level: cII p22
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0053/annotation2042/range2042/1

https://synbiohub.org/public/igem/BBa_R0053/annotation2042/range2042/1
type: Range
persistent identity: range2042
display id: range2042
version: 1
top level: cII p22
direction: direction/forward
start: 30
end: 35
orientation: inline

BBa_R0053
type: SequenceAnnotation
persistent identity: annotation7069
display id: annotation7069
version: 1
top level: cII p22
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_R0053/annotation7069/range7069/1

https://synbiohub.org/public/igem/BBa_R0053/annotation7069/range7069/1
type: Range
persistent identity: range7069
display id: range7069
version: 1
top level: cII p22
direction: direction/forward
start: 1
end: 54
orientation: inline

OR2
type: SequenceAnnotation
persistent identity: annotation2036
display id: annotation2036
version: 1
top level: cII p22
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0053/annotation2036/range2036/1

https://synbiohub.org/public/igem/BBa_R0053/annotation2036/range2036/1
type: Range
persistent identity: range2036
display id: range2036
version: 1
top level: cII p22
direction: direction/forward
start: 11
end: 28
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2041
display id: annotation2041
version: 1
top level: cII p22
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0053/annotation2041/range2041/1

https://synbiohub.org/public/igem/BBa_R0053/annotation2041/range2041/1
type: Range
persistent identity: range2041
display id: range2041
version: 1
top level: cII p22
direction: direction/forward
start: 8
end: 13
orientation: inline

Lambda att
type: ComponentDefinition
persistent identity: BBa I11023
display id: BBa_I11023
version: 1
was generated by: iGEM to SBOL conversion
description: Lambda attP
created: 2004-09-02T11:00:00Z
modified: 2015-08-31T04:07:30Z
owned by: james
owned by: myers
mutable description: Lambda phage attP binding site P-O-P'
discontinued: false
dominant: false
experience: experience/None
group u list: _2_
m user id: 0
owner id: 102
owning group id: 7
sample status: It's complicated
status: status/Planning
bookmark: false
star: false
creator: bu
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: lambda phage attp site
sequence: https://synbiohub.org/public/igem/BBa_I11023_sequence/1

lambda phage attp site
type: SequenceAnnotation
persistent identity: annotation1064123
display id: annotation1064123
version: 1
top level: Lambda att
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I11023/annotation1064123/range1064123/1

https://synbiohub.org/public/igem/BBa_I11023/annotation1064123/range1064123/1
type: Range
persistent identity: range1064123
display id: range1064123
version: 1
top level: Lambda att
direction: direction/forward
start: 1
end: 232
orientation: inline

BBa_Z0251
type: ComponentDefinition
persistent identity: BBa Z0251
display id: BBa_Z0251
version: 1
was generated by: iGEM to SBOL conversion
description: T7 strong promoter
created: 2005-04-04T11:00:00Z
modified: 2015-05-08T01:14:55Z
mutable provenance: bacteriophage T7
owned by: james
owned by: myers
mutable description: Promoter for T7 RNA polymerase
discontinued: false
dominant: false
experience: experience/None
group u list: _10_
m user id: 0
owner id: 250
owning group id: 10
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: T7.2
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: consensus sequence
sequence: https://synbiohub.org/public/igem/BBa_Z0251_sequence/1

consensus sequence
type: SequenceAnnotation
persistent identity: annotation1476333
display id: annotation1476333
version: 1
top level: BBa_Z0251
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_Z0251/annotation1476333/range1476333/1

https://synbiohub.org/public/igem/BBa_Z0251/annotation1476333/range1476333/1
type: Range
persistent identity: range1476333
display id: range1476333
version: 1
top level: BBa_Z0251
direction: direction/forward
start: 6
end: 28
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

BBa_Y1016
type: ComponentDefinition
persistent identity: BBa Y1016
display id: BBa_Y1016
version: 1
was generated by: iGEM to SBOL conversion
description: E.coli origin
created: 2005-06-22T11:00:00Z
modified: 2015-05-08T01:14:54Z
owned by: james
owned by: myers
mutable description: Bacterial origin
discontinued: false
dominant: false
experience: experience/None
group u list: _11_
m user id: 0
owner id: 351
owning group id: 43
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Samantha Sutton
type: DnaRegion
role: partType/Other
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_Y1016_sequence/1

BBa_Y1009
type: ComponentDefinition
persistent identity: BBa Y1009
display id: BBa_Y1009
version: 1
was generated by: iGEM to SBOL conversion
description: Trp1 Gene
created: 2005-06-22T11:00:00Z
modified: 2015-05-08T01:14:54Z
owned by: james
owned by: myers
mutable description: Has its own promoter and terminator
discontinued: false
dominant: false
experience: experience/None
group u list: _11_
m user id: 0
owner id: 351
owning group id: 43
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Samantha Sutton
type: DnaRegion
role: partType/Other
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_Y1009_sequence/1

BBa_Y1006
type: ComponentDefinition
persistent identity: BBa Y1006
display id: BBa_Y1006
version: 1
was generated by: iGEM to SBOL conversion
description: His3 Gene
created: 2005-06-22T11:00:00Z
modified: 2015-05-08T01:14:54Z
owned by: james
owned by: myers
mutable description: Has its own promoter and terminator
discontinued: false
dominant: false
experience: experience/None
group u list: _11_
m user id: 0
owner id: 351
owning group id: 43
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Samantha Sutton
type: DnaRegion
role: partType/Other
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_Y1006_sequence/1

eyfp
type: ComponentDefinition
persistent identity: BBa E0030
display id: BBa_E0030
version: 1
was generated by: iGEM to SBOL conversion
description: enhanced yellow fluorescent protein derived from A. victoria GFP
created: 2004-03-02T12:00:00Z
modified: 2015-08-31T04:07:25Z
mutable provenance: Modificaitons to Clontech EYFP by Reshma Shetty
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: -- No description --
discontinued: false
dominant: false
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Caitlin Conboy and Jennifer Braff
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_E0030_sequence/1

BBa_Y1007
type: ComponentDefinition
persistent identity: BBa Y1007
display id: BBa_Y1007
version: 1
was generated by: iGEM to SBOL conversion
description: Ura3 Gene
created: 2005-06-22T11:00:00Z
modified: 2015-05-08T01:14:54Z
owned by: james
owned by: myers
mutable description: Has its own promoter and terminator
discontinued: false
dominant: true
experience: experience/None
group u list: _11_
m user id: 0
owner id: 351
owning group id: 43
sample status: It's complicated
status: status/Planning
bookmark: false
star: false
creator: Samantha Sutton
type: DnaRegion
role: partType/Other
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_Y1007_sequence/1

BBa_J07006
type: ComponentDefinition
persistent identity: BBa J07006
display id: BBa_J07006
version: 1
was generated by: iGEM to SBOL conversion
description: malE
created: 2005-07-05T11:00:00Z
modified: 2015-08-31T04:08:19Z
mutable provenance: Genbank E.coli K-12 strain
owned by: james
owned by: myers
mutable description: malE - a monomer. This monomeric protein is used as a negative control when testing for dimerization.
discontinued: false
dominant: false
experience: experience/None
group u list: _21_
m user id: 0
owner id: 379
owning group id: 21
sample status: It's complicated
status: status/Planning
bookmark: false
star: false
creator: igem_mit_2005
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: malE
sequence: https://synbiohub.org/public/igem/BBa_J07006_sequence/1

malE
type: SequenceAnnotation
persistent identity: annotation1569704
display id: annotation1569704
version: 1
top level: BBa_J07006
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J07006/annotation1569704/range1569704/1

https://synbiohub.org/public/igem/BBa_J07006/annotation1569704/range1569704/1
type: Range
persistent identity: range1569704
display id: range1569704
version: 1
top level: BBa_J07006
direction: direction/forward
start: 1
end: 1191
orientation: inline

BBa_Y1011
type: ComponentDefinition
persistent identity: BBa Y1011
display id: BBa_Y1011
version: 1
was generated by: iGEM to SBOL conversion
description: Act1 Promoter
created: 2005-06-22T11:00:00Z
modified: 2015-05-08T01:14:54Z
owned by: james
owned by: myers
mutable description: Promoter
discontinued: false
dominant: false
experience: experience/None
group u list: _11_
m user id: 0
owner id: 351
owning group id: 43
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Samantha Sutton
type: DnaRegion
role: partType/Other
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_Y1011_sequence/1

p(tetR)
type: ComponentDefinition
persistent identity: BBa R0040
display id: BBa_R0040
version: 1
was generated by: iGEM to SBOL conversion
description: TetR repressible promoter
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: Lutz, R., Bujard, H., Nucleic Acids Research (1997) 25, 1203-1210.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Sequence for pTet inverting regulator driven by the TetR protein.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: BBa_R0040 TetR-Regulated Promoter is based on a cI promoter. It has been modified to include two TetR binding sites and the BioBrick standard assembly head and tail restriction sites.
star: true
creator: June Rhee, Connie Tao, Ty Thomson, Louis Waldman
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -35
sequence annotation: TetR 2
sequence annotation: BBa_R0040
sequence annotation: TetR 1
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R0040_sequence/1

-35
type: SequenceAnnotation
persistent identity: annotation1986785
display id: annotation1986785
version: 1
top level: p(tetR)
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986785/range1986785/1

https://synbiohub.org/public/igem/BBa_R0040/annotation1986785/range1986785/1
type: Range
persistent identity: range1986785
display id: range1986785
version: 1
top level: p(tetR)
direction: direction/forward
start: 20
end: 25
orientation: inline

TetR 2
type: SequenceAnnotation
persistent identity: annotation1986786
display id: annotation1986786
version: 1
top level: p(tetR)
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986786/range1986786/1

https://synbiohub.org/public/igem/BBa_R0040/annotation1986786/range1986786/1
type: Range
persistent identity: range1986786
display id: range1986786
version: 1
top level: p(tetR)
direction: direction/forward
start: 26
end: 44
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1986784
display id: annotation1986784
version: 1
top level: p(tetR)
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986784/range1986784/1

https://synbiohub.org/public/igem/BBa_R0040/annotation1986784/range1986784/1
type: Range
persistent identity: range1986784
display id: range1986784
version: 1
top level: p(tetR)
direction: direction/forward
start: 1
end: 54
orientation: inline

TetR 1
type: SequenceAnnotation
persistent identity: annotation1986783
display id: annotation1986783
version: 1
top level: p(tetR)
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986783/range1986783/1

https://synbiohub.org/public/igem/BBa_R0040/annotation1986783/range1986783/1
type: Range
persistent identity: range1986783
display id: range1986783
version: 1
top level: p(tetR)
direction: direction/forward
start: 1
end: 19
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1986787
display id: annotation1986787
version: 1
top level: p(tetR)
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986787/range1986787/1

https://synbiohub.org/public/igem/BBa_R0040/annotation1986787/range1986787/1
type: Range
persistent identity: range1986787
display id: range1986787
version: 1
top level: p(tetR)
direction: direction/forward
start: 43
end: 48
orientation: inline

Message
type: ComponentDefinition
persistent identity: BBa K361001
display id: BBa_K361001
version: 1
was generated by: iGEM to SBOL conversion
description: Message gene template
created: 2010-10-23T11:00:00Z
modified: 2015-05-08T01:12:12Z
mutable provenance: This artificial DNA sequence is converted from text with the use of ASCII system. The specific repeat sequence "repeat 1" and "repeat 2" are come from transfer region in R64 plasmid, with genomic assession no.:AB027308.1
owned by: james
owned by: myers
mutable description: This message system consists of a unique designated message sequence, being inserted with specific repeat sequence for recognition site by Rci site-specific recombinase.
discontinued: false
dominant: false
experience: experience/None
group u list: _476_
m user id: 0
owner id: 6123
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: This DNA sequence is acting as a template, to guide the make of a message inserted with specific repeat sequence for recognition by Rci recombinase in order to accomplish random rearrangement of message in encryption process.
star: false
creator: Jacky, Fong Chuen, Loo
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: Footer
sequence annotation: Header
sequence annotation: repeat 1
sequence annotation: repeat 2
sequence: https://synbiohub.org/public/igem/BBa_K361001_sequence/1

Footer
type: SequenceAnnotation
persistent identity: annotation2095201
display id: annotation2095201
version: 1
top level: Message
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K361001/annotation2095201/range2095201/1

https://synbiohub.org/public/igem/BBa_K361001/annotation2095201/range2095201/1
type: Range
persistent identity: range2095201
display id: range2095201
version: 1
top level: Message
direction: direction/forward
start: 363
end: 426
orientation: inline

Header
type: SequenceAnnotation
persistent identity: annotation2095196
display id: annotation2095196
version: 1
top level: Message
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K361001/annotation2095196/range2095196/1

https://synbiohub.org/public/igem/BBa_K361001/annotation2095196/range2095196/1
type: Range
persistent identity: range2095196
display id: range2095196
version: 1
top level: Message
direction: direction/forward
start: 13
end: 20
orientation: inline

repeat 1
type: SequenceAnnotation
persistent identity: annotation2095198
display id: annotation2095198
version: 1
top level: Message
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K361001/annotation2095198/range2095198/1

https://synbiohub.org/public/igem/BBa_K361001/annotation2095198/range2095198/1
type: Range
persistent identity: range2095198
display id: range2095198
version: 1
top level: Message
direction: direction/forward
start: 21
end: 51
orientation: inline

repeat 2
type: SequenceAnnotation
persistent identity: annotation2095200
display id: annotation2095200
version: 1
top level: Message
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K361001/annotation2095200/range2095200/1

https://synbiohub.org/public/igem/BBa_K361001/annotation2095200/range2095200/1
type: Range
persistent identity: range2095200
display id: range2095200
version: 1
top level: Message
direction: direction/forward
start: 332
end: 362
orientation: inline

BBa_I11042
type: ComponentDefinition
persistent identity: BBa I11042
display id: BBa_I11042
version: 1
was generated by: iGEM to SBOL conversion
description: HK022 attB rev comp
created: 2004-09-06T11:00:00Z
modified: 2015-08-31T04:07:30Z
mutable provenance: J Bacteriol, 1990 Sep;172(11):6540-6550
owned by: james
owned by: myers
mutable description: Reverse complement HK022 attB attachment site
discontinued: false
dominant: false
experience: experience/None
group u list: _2_
m user id: 0
owner id: 102
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: bu
type: DnaRegion
role: partType/Other
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_I11042_sequence/1

cI lam
type: ComponentDefinition
persistent identity: BBa R0051
display id: BBa_R0051
version: 1
was generated by: iGEM to SBOL conversion
description: promoter (lambda cI regulated)
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: A synthetic oscillatory network of transcriptional regulators , Elowitz M.B. , Leibler S., Nature(403),335-38: 2000
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: The cI regulated promoter is based on the pR promtoer from bacteriohage lambda. The promoter has two two DNA binding sites for lambda cI repressor <bb_part>BBa_C0051</bb_part>. cI binding results in repression of transcription. The specific sequence used here is based on the cI repressible promoter used in the Elowitz repressilator (and references therein).
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: In order to address concerns about the promoter transcribing in the reverse direction, we have removed the -35 and -10 signals responsible for the promoter activity in the reverse direction. (More details needed here! DE, 2/24/03) Incompatible with host expressing cI repressor.
star: true
creator: Vinay S Mahajan, Brian Chow, Peter Carr, Voichita Marinescu and Alexander D. Wissner-Gross
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -35
sequence annotation: -10
sequence annotation: OR2
sequence annotation: BBa_R0051
sequence annotation: OR1
sequence: https://synbiohub.org/public/igem/BBa_R0051_sequence/1

-35
type: SequenceAnnotation
persistent identity: annotation2023
display id: annotation2023
version: 1
top level: cI lam
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0051/annotation2023/range2023/1

https://synbiohub.org/public/igem/BBa_R0051/annotation2023/range2023/1
type: Range
persistent identity: range2023
display id: range2023
version: 1
top level: cI lam
direction: direction/forward
start: 15
end: 20
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2022
display id: annotation2022
version: 1
top level: cI lam
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0051/annotation2022/range2022/1

https://synbiohub.org/public/igem/BBa_R0051/annotation2022/range2022/1
type: Range
persistent identity: range2022
display id: range2022
version: 1
top level: cI lam
direction: direction/forward
start: 38
end: 43
orientation: inline

OR2
type: SequenceAnnotation
persistent identity: annotation2025
display id: annotation2025
version: 1
top level: cI lam
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0051/annotation2025/range2025/1

https://synbiohub.org/public/igem/BBa_R0051/annotation2025/range2025/1
type: Range
persistent identity: range2025
display id: range2025
version: 1
top level: cI lam
direction: direction/forward
start: 1
end: 17
orientation: inline

BBa_R0051
type: SequenceAnnotation
persistent identity: annotation7067
display id: annotation7067
version: 1
top level: cI lam
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_R0051/annotation7067/range7067/1

https://synbiohub.org/public/igem/BBa_R0051/annotation7067/range7067/1
type: Range
persistent identity: range7067
display id: range7067
version: 1
top level: cI lam
direction: direction/forward
start: 1
end: 49
orientation: inline

OR1
type: SequenceAnnotation
persistent identity: annotation2024
display id: annotation2024
version: 1
top level: cI lam
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0051/annotation2024/range2024/1

https://synbiohub.org/public/igem/BBa_R0051/annotation2024/range2024/1
type: Range
persistent identity: range2024
display id: range2024
version: 1
top level: cI lam
direction: direction/forward
start: 25
end: 41
orientation: inline

BBa_Y1008
type: ComponentDefinition
persistent identity: BBa Y1008
display id: BBa_Y1008
version: 1
was generated by: iGEM to SBOL conversion
description: Leu2 Gene
created: 2005-06-22T11:00:00Z
modified: 2015-05-08T01:14:54Z
owned by: james
owned by: myers
mutable description: Has its own promoter and terminator
discontinued: false
dominant: false
experience: experience/None
group u list: _11_
m user id: 0
owner id: 351
owning group id: 43
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Samantha Sutton
type: DnaRegion
role: partType/Other
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_Y1008_sequence/1

BBa_J07002
type: ComponentDefinition
persistent identity: BBa J07002
display id: BBa_J07002
version: 1
was generated by: iGEM to SBOL conversion
description: abandoned test construct
created: 2005-06-15T11:00:00Z
modified: 2015-08-31T04:08:19Z
owned by: james
owned by: myers
discontinued: false
dominant: false
experience: experience/None
group u list: _21_
m user id: 0
owner id: 350
owning group id: 21
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Jenny
type: DnaRegion
role: partType/Other
role: SO:0000110
component: BBa_C0012
component: BBa_B0012
component: BBa_B0010
component: BBa_R0051
component: BBa_R0011
component: BBa_R0040
component: BBa_B0034
component: BBa_C0051
component: BBa_E0030
sequence annotation: BBa_B0010
sequence annotation: BBa_B0034
sequence annotation: BBa_R0011
sequence annotation: BBa_B0012
sequence annotation: BBa_C0051
sequence annotation: BBa_R0040
sequence annotation: BBa_R0051
sequence annotation: BBa_C0012
sequence annotation: BBa_E0030
sequence: https://synbiohub.org/public/igem/BBa_J07002_sequence/1

BBa_C0012
type: Component
persistent identity: component1522807
display id: component1522807
version: 1
top level: BBa_J07002
access: public
definition: lacI

BBa_B0012
type: Component
persistent identity: component1522824
display id: component1522824
version: 1
top level: BBa_J07002
access: public
definition: BBa_B0012

BBa_B0010
type: Component
persistent identity: component1522903
display id: component1522903
version: 1
top level: BBa_J07002
access: public
definition: BBa_B0010

BBa_B0010
type: Component
persistent identity: component1522814
display id: component1522814
version: 1
top level: BBa_J07002
access: public
definition: BBa_B0010

BBa_R0051
type: Component
persistent identity: component1522888
display id: component1522888
version: 1
top level: BBa_J07002
access: public
definition: cI lam

BBa_R0011
type: Component
persistent identity: component1522838
display id: component1522838
version: 1
top level: BBa_J07002
access: public
definition: lacI+pL

BBa_B0010
type: Component
persistent identity: component1522863
display id: component1522863
version: 1
top level: BBa_J07002
access: public
definition: BBa_B0010

BBa_R0040
type: Component
persistent identity: component1522789
display id: component1522789
version: 1
top level: BBa_J07002
access: public
definition: p(tetR)

BBa_B0034
type: Component
persistent identity: component1522797
display id: component1522797
version: 1
top level: BBa_J07002
access: public
definition: BBa_B0034

BBa_B0012
type: Component
persistent identity: component1522873
display id: component1522873
version: 1
top level: BBa_J07002
access: public
definition: BBa_B0012

BBa_B0012
type: Component
persistent identity: component1522913
display id: component1522913
version: 1
top level: BBa_J07002
access: public
definition: BBa_B0012

BBa_C0051
type: Component
persistent identity: component1522857
display id: component1522857
version: 1
top level: BBa_J07002
access: public
definition: cI lam

BBa_B0034
type: Component
persistent identity: component1522847
display id: component1522847
version: 1
top level: BBa_J07002
access: public
definition: BBa_B0034

BBa_E0030
type: Component
persistent identity: component1522898
display id: component1522898
version: 1
top level: BBa_J07002
access: public
definition: eyfp

BBa_B0034
type: Component
persistent identity: component1522896
display id: component1522896
version: 1
top level: BBa_J07002
access: public
definition: BBa_B0034

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1522814
display id: annotation1522814
version: 1
top level: BBa_J07002
location: https://synbiohub.org/public/igem/BBa_J07002/annotation1522814/range1522814/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_J07002/annotation1522814/range1522814/1
type: Range
persistent identity: range1522814
display id: range1522814
version: 1
top level: BBa_J07002
direction: direction/forward
start: 1242
end: 1321
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1522896
display id: annotation1522896
version: 1
top level: BBa_J07002
location: https://synbiohub.org/public/igem/BBa_J07002/annotation1522896/range1522896/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_J07002/annotation1522896/range1522896/1
type: Range
persistent identity: range1522896
display id: range1522896
version: 1
top level: BBa_J07002
direction: direction/forward
start: 2437
end: 2448
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1522903
display id: annotation1522903
version: 1
top level: BBa_J07002
location: https://synbiohub.org/public/igem/BBa_J07002/annotation1522903/range1522903/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_J07002/annotation1522903/range1522903/1
type: Range
persistent identity: range1522903
display id: range1522903
version: 1
top level: BBa_J07002
direction: direction/forward
start: 3186
end: 3265
orientation: inline

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation1522838
display id: annotation1522838
version: 1
top level: BBa_J07002
location: https://synbiohub.org/public/igem/BBa_J07002/annotation1522838/range1522838/1
component: BBa_R0011

https://synbiohub.org/public/igem/BBa_J07002/annotation1522838/range1522838/1
type: Range
persistent identity: range1522838
display id: range1522838
version: 1
top level: BBa_J07002
direction: direction/forward
start: 1379
end: 1432
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1522873
display id: annotation1522873
version: 1
top level: BBa_J07002
location: https://synbiohub.org/public/igem/BBa_J07002/annotation1522873/range1522873/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_J07002/annotation1522873/range1522873/1
type: Range
persistent identity: range1522873
display id: range1522873
version: 1
top level: BBa_J07002
direction: direction/forward
start: 2331
end: 2371
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1522913
display id: annotation1522913
version: 1
top level: BBa_J07002
location: https://synbiohub.org/public/igem/BBa_J07002/annotation1522913/range1522913/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_J07002/annotation1522913/range1522913/1
type: Range
persistent identity: range1522913
display id: range1522913
version: 1
top level: BBa_J07002
direction: direction/forward
start: 3274
end: 3314
orientation: inline

BBa_C0051
type: SequenceAnnotation
persistent identity: annotation1522857
display id: annotation1522857
version: 1
top level: BBa_J07002
location: https://synbiohub.org/public/igem/BBa_J07002/annotation1522857/range1522857/1
component: BBa_C0051

https://synbiohub.org/public/igem/BBa_J07002/annotation1522857/range1522857/1
type: Range
persistent identity: range1522857
display id: range1522857
version: 1
top level: BBa_J07002
direction: direction/forward
start: 1460
end: 2209
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1522824
display id: annotation1522824
version: 1
top level: BBa_J07002
location: https://synbiohub.org/public/igem/BBa_J07002/annotation1522824/range1522824/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_J07002/annotation1522824/range1522824/1
type: Range
persistent identity: range1522824
display id: range1522824
version: 1
top level: BBa_J07002
direction: direction/forward
start: 1330
end: 1370
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1522863
display id: annotation1522863
version: 1
top level: BBa_J07002
location: https://synbiohub.org/public/igem/BBa_J07002/annotation1522863/range1522863/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_J07002/annotation1522863/range1522863/1
type: Range
persistent identity: range1522863
display id: range1522863
version: 1
top level: BBa_J07002
direction: direction/forward
start: 2243
end: 2322
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1522789
display id: annotation1522789
version: 1
top level: BBa_J07002
location: https://synbiohub.org/public/igem/BBa_J07002/annotation1522789/range1522789/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_J07002/annotation1522789/range1522789/1
type: Range
persistent identity: range1522789
display id: range1522789
version: 1
top level: BBa_J07002
direction: direction/forward
start: 1
end: 54
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1522847
display id: annotation1522847
version: 1
top level: BBa_J07002
location: https://synbiohub.org/public/igem/BBa_J07002/annotation1522847/range1522847/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_J07002/annotation1522847/range1522847/1
type: Range
persistent identity: range1522847
display id: range1522847
version: 1
top level: BBa_J07002
direction: direction/forward
start: 1442
end: 1453
orientation: inline

BBa_R0051
type: SequenceAnnotation
persistent identity: annotation1522888
display id: annotation1522888
version: 1
top level: BBa_J07002
location: https://synbiohub.org/public/igem/BBa_J07002/annotation1522888/range1522888/1
component: BBa_R0051

https://synbiohub.org/public/igem/BBa_J07002/annotation1522888/range1522888/1
type: Range
persistent identity: range1522888
display id: range1522888
version: 1
top level: BBa_J07002
direction: direction/forward
start: 2380
end: 2428
orientation: inline

BBa_C0012
type: SequenceAnnotation
persistent identity: annotation1522807
display id: annotation1522807
version: 1
top level: BBa_J07002
location: https://synbiohub.org/public/igem/BBa_J07002/annotation1522807/range1522807/1
component: BBa_C0012

https://synbiohub.org/public/igem/BBa_J07002/annotation1522807/range1522807/1
type: Range
persistent identity: range1522807
display id: range1522807
version: 1
top level: BBa_J07002
direction: direction/forward
start: 81
end: 1208
orientation: inline

BBa_E0030
type: SequenceAnnotation
persistent identity: annotation1522898
display id: annotation1522898
version: 1
top level: BBa_J07002
location: https://synbiohub.org/public/igem/BBa_J07002/annotation1522898/range1522898/1
component: BBa_E0030

https://synbiohub.org/public/igem/BBa_J07002/annotation1522898/range1522898/1
type: Range
persistent identity: range1522898
display id: range1522898
version: 1
top level: BBa_J07002
direction: direction/forward
start: 2455
end: 3177
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1522797
display id: annotation1522797
version: 1
top level: BBa_J07002
location: https://synbiohub.org/public/igem/BBa_J07002/annotation1522797/range1522797/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_J07002/annotation1522797/range1522797/1
type: Range
persistent identity: range1522797
display id: range1522797
version: 1
top level: BBa_J07002
direction: direction/forward
start: 63
end: 74
orientation: inline

cI lam
type: ComponentDefinition
persistent identity: BBa C0051
display id: BBa_C0051
version: 1
was generated by: iGEM to SBOL conversion
description: cI repressor from E. coli phage lambda (+LVA)
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:23Z
mutable provenance: Elowitz, M. B. Transport, Assembly, and Dynamics in Systems of Interacting Proteins. Thesis, Princeton Univ., Princeton (1999).
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Coding region for the cI repressor based on cI repressor from bacteriophage lambda modified with an LVA tail for rapid degradation of the protein. cI repressor binds to the cI regulator (BBa_R0051).
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: References (unparsed) here: A synthetic oscillatory network of transcriptional regulators , Elowitz M.B. , Leibler S., Nature(403),335-38: 2000 Octamerization of CI repressor is needed for effective repression of PRM and efficient switching from lysogeny. Ian B. Dodd,1 Alison J. Perkins, Daniel Tsemitsidis, and J. Barry Egan , Genes and Development (Vol 15, No. 22) 3013-3022: 2001 References (unparsed) here: A synthetic oscillatory network of transcriptional regulators , Elowitz M.B. , Leibler S., Nature(403),335-38: 2000 Octamerization of CI repressor is needed for effective repression of PRM and efficient switching from lysogeny. Ian B. Dodd,1 Alison J. Perkins, Daniel Tsemitsidis, and J. Barry Egan , Genes and Development (Vol 15, No. 22) 3013-3022: 2001 BBa_C0051 cI repressor is based on the cI repressor from the Elowitz's repressilator. It has been modified to include a rapid degradation LAA tail, and includes the BioBrick standard assembly head and tail restriction sites. The RBS has been removed. The stop codon has been changed from TAA to a double stop codon TAATAA.
star: true
creator: Vinay S Mahajan, Brian Chow, Peter Carr, Voichita Marinescu and Alexander D. Wissner-Gross
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: LVA
sequence annotation: cI lambda
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C0051_sequence/1

LVA
type: SequenceAnnotation
persistent identity: annotation23335
display id: annotation23335
version: 1
top level: cI lam
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_C0051/annotation23335/range23335/1

https://synbiohub.org/public/igem/BBa_C0051/annotation23335/range23335/1
type: Range
persistent identity: range23335
display id: range23335
version: 1
top level: cI lam
direction: direction/forward
start: 712
end: 744
orientation: inline

cI lambda
type: SequenceAnnotation
persistent identity: annotation23334
display id: annotation23334
version: 1
top level: cI lam
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_C0051/annotation23334/range23334/1

https://synbiohub.org/public/igem/BBa_C0051/annotation23334/range23334/1
type: Range
persistent identity: range23334
display id: range23334
version: 1
top level: cI lam
direction: direction/forward
start: 4
end: 711
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213991
display id: annotation2213991
version: 1
top level: cI lam
role: SO:0000807
role: feature/barcode
location: https://synbiohub.org/public/igem/BBa_C0051/annotation2213991/range2213991/1

https://synbiohub.org/public/igem/BBa_C0051/annotation2213991/range2213991/1
type: Range
persistent identity: range2213991
display id: range2213991
version: 1
top level: cI lam
direction: direction/forward
start: 751
end: 775
orientation: inline

BBa_J07000
type: ComponentDefinition
persistent identity: BBa J07000
display id: BBa_J07000
version: 1
was generated by: iGEM to SBOL conversion
description: abandoned test construct
created: 2005-06-15T11:00:00Z
modified: 2015-08-31T04:08:19Z
owned by: james
owned by: myers
discontinued: false
dominant: false
experience: experience/None
group u list: _21_
m user id: 0
owner id: 350
owning group id: 21
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Jenny
type: DnaRegion
role: partType/Other
role: SO:0000110
component: BBa_R0053
component: BBa_B0012
component: BBa_B0034
component: BBa_B0010
component: BBa_C0051
component: BBa_C0012
component: BBa_R0011
component: BBa_R0051
component: BBa_E0030
sequence annotation: BBa_B0034
sequence annotation: BBa_E0030
sequence annotation: BBa_B0010
sequence annotation: BBa_R0051
sequence annotation: BBa_R0011
sequence annotation: BBa_B0012
sequence annotation: BBa_C0051
sequence annotation: BBa_C0012
sequence annotation: BBa_R0053
sequence: https://synbiohub.org/public/igem/BBa_J07000_sequence/1

BBa_R0053
type: Component
persistent identity: component1521549
display id: component1521549
version: 1
top level: BBa_J07000
access: public
definition: cII p22

BBa_B0012
type: Component
persistent identity: component1521675
display id: component1521675
version: 1
top level: BBa_J07000
access: public
definition: BBa_B0012

BBa_B0034
type: Component
persistent identity: component1521609
display id: component1521609
version: 1
top level: BBa_J07000
access: public
definition: BBa_B0034

BBa_B0010
type: Component
persistent identity: component1521625
display id: component1521625
version: 1
top level: BBa_J07000
access: public
definition: BBa_B0010

BBa_B0034
type: Component
persistent identity: component1521559
display id: component1521559
version: 1
top level: BBa_J07000
access: public
definition: BBa_B0034

BBa_C0051
type: Component
persistent identity: component1521619
display id: component1521619
version: 1
top level: BBa_J07000
access: public
definition: cI lam

BBa_C0012
type: Component
persistent identity: component1521569
display id: component1521569
version: 1
top level: BBa_J07000
access: public
definition: lacI

BBa_B0010
type: Component
persistent identity: component1521576
display id: component1521576
version: 1
top level: BBa_J07000
access: public
definition: BBa_B0010

BBa_R0011
type: Component
persistent identity: component1521600
display id: component1521600
version: 1
top level: BBa_J07000
access: public
definition: lacI+pL

BBa_B0012
type: Component
persistent identity: component1521635
display id: component1521635
version: 1
top level: BBa_J07000
access: public
definition: BBa_B0012

BBa_R0051
type: Component
persistent identity: component1521650
display id: component1521650
version: 1
top level: BBa_J07000
access: public
definition: cI lam

BBa_B0010
type: Component
persistent identity: component1521665
display id: component1521665
version: 1
top level: BBa_J07000
access: public
definition: BBa_B0010

BBa_E0030
type: Component
persistent identity: component1521660
display id: component1521660
version: 1
top level: BBa_J07000
access: public
definition: eyfp

BBa_B0034
type: Component
persistent identity: component1521658
display id: component1521658
version: 1
top level: BBa_J07000
access: public
definition: BBa_B0034

BBa_B0012
type: Component
persistent identity: component1521586
display id: component1521586
version: 1
top level: BBa_J07000
access: public
definition: BBa_B0012

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1521609
display id: annotation1521609
version: 1
top level: BBa_J07000
location: https://synbiohub.org/public/igem/BBa_J07000/annotation1521609/range1521609/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_J07000/annotation1521609/range1521609/1
type: Range
persistent identity: range1521609
display id: range1521609
version: 1
top level: BBa_J07000
direction: direction/forward
start: 1442
end: 1453
orientation: inline

BBa_E0030
type: SequenceAnnotation
persistent identity: annotation1521660
display id: annotation1521660
version: 1
top level: BBa_J07000
location: https://synbiohub.org/public/igem/BBa_J07000/annotation1521660/range1521660/1
component: BBa_E0030

https://synbiohub.org/public/igem/BBa_J07000/annotation1521660/range1521660/1
type: Range
persistent identity: range1521660
display id: range1521660
version: 1
top level: BBa_J07000
direction: direction/forward
start: 2455
end: 3177
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1521665
display id: annotation1521665
version: 1
top level: BBa_J07000
location: https://synbiohub.org/public/igem/BBa_J07000/annotation1521665/range1521665/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_J07000/annotation1521665/range1521665/1
type: Range
persistent identity: range1521665
display id: range1521665
version: 1
top level: BBa_J07000
direction: direction/forward
start: 3186
end: 3265
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1521559
display id: annotation1521559
version: 1
top level: BBa_J07000
location: https://synbiohub.org/public/igem/BBa_J07000/annotation1521559/range1521559/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_J07000/annotation1521559/range1521559/1
type: Range
persistent identity: range1521559
display id: range1521559
version: 1
top level: BBa_J07000
direction: direction/forward
start: 63
end: 74
orientation: inline

BBa_R0051
type: SequenceAnnotation
persistent identity: annotation1521650
display id: annotation1521650
version: 1
top level: BBa_J07000
location: https://synbiohub.org/public/igem/BBa_J07000/annotation1521650/range1521650/1
component: BBa_R0051

https://synbiohub.org/public/igem/BBa_J07000/annotation1521650/range1521650/1
type: Range
persistent identity: range1521650
display id: range1521650
version: 1
top level: BBa_J07000
direction: direction/forward
start: 2380
end: 2428
orientation: inline

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation1521600
display id: annotation1521600
version: 1
top level: BBa_J07000
location: https://synbiohub.org/public/igem/BBa_J07000/annotation1521600/range1521600/1
component: BBa_R0011

https://synbiohub.org/public/igem/BBa_J07000/annotation1521600/range1521600/1
type: Range
persistent identity: range1521600
display id: range1521600
version: 1
top level: BBa_J07000
direction: direction/forward
start: 1379
end: 1432
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1521576
display id: annotation1521576
version: 1
top level: BBa_J07000
location: https://synbiohub.org/public/igem/BBa_J07000/annotation1521576/range1521576/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_J07000/annotation1521576/range1521576/1
type: Range
persistent identity: range1521576
display id: range1521576
version: 1
top level: BBa_J07000
direction: direction/forward
start: 1242
end: 1321
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1521635
display id: annotation1521635
version: 1
top level: BBa_J07000
location: https://synbiohub.org/public/igem/BBa_J07000/annotation1521635/range1521635/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_J07000/annotation1521635/range1521635/1
type: Range
persistent identity: range1521635
display id: range1521635
version: 1
top level: BBa_J07000
direction: direction/forward
start: 2331
end: 2371
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1521658
display id: annotation1521658
version: 1
top level: BBa_J07000
location: https://synbiohub.org/public/igem/BBa_J07000/annotation1521658/range1521658/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_J07000/annotation1521658/range1521658/1
type: Range
persistent identity: range1521658
display id: range1521658
version: 1
top level: BBa_J07000
direction: direction/forward
start: 2437
end: 2448
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1521625
display id: annotation1521625
version: 1
top level: BBa_J07000
location: https://synbiohub.org/public/igem/BBa_J07000/annotation1521625/range1521625/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_J07000/annotation1521625/range1521625/1
type: Range
persistent identity: range1521625
display id: range1521625
version: 1
top level: BBa_J07000
direction: direction/forward
start: 2243
end: 2322
orientation: inline

BBa_C0051
type: SequenceAnnotation
persistent identity: annotation1521619
display id: annotation1521619
version: 1
top level: BBa_J07000
location: https://synbiohub.org/public/igem/BBa_J07000/annotation1521619/range1521619/1
component: BBa_C0051

https://synbiohub.org/public/igem/BBa_J07000/annotation1521619/range1521619/1
type: Range
persistent identity: range1521619
display id: range1521619
version: 1
top level: BBa_J07000
direction: direction/forward
start: 1460
end: 2209
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1521586
display id: annotation1521586
version: 1
top level: BBa_J07000
location: https://synbiohub.org/public/igem/BBa_J07000/annotation1521586/range1521586/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_J07000/annotation1521586/range1521586/1
type: Range
persistent identity: range1521586
display id: range1521586
version: 1
top level: BBa_J07000
direction: direction/forward
start: 1330
end: 1370
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1521675
display id: annotation1521675
version: 1
top level: BBa_J07000
location: https://synbiohub.org/public/igem/BBa_J07000/annotation1521675/range1521675/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_J07000/annotation1521675/range1521675/1
type: Range
persistent identity: range1521675
display id: range1521675
version: 1
top level: BBa_J07000
direction: direction/forward
start: 3274
end: 3314
orientation: inline

BBa_C0012
type: SequenceAnnotation
persistent identity: annotation1521569
display id: annotation1521569
version: 1
top level: BBa_J07000
location: https://synbiohub.org/public/igem/BBa_J07000/annotation1521569/range1521569/1
component: BBa_C0012

https://synbiohub.org/public/igem/BBa_J07000/annotation1521569/range1521569/1
type: Range
persistent identity: range1521569
display id: range1521569
version: 1
top level: BBa_J07000
direction: direction/forward
start: 81
end: 1208
orientation: inline

BBa_R0053
type: SequenceAnnotation
persistent identity: annotation1521549
display id: annotation1521549
version: 1
top level: BBa_J07000
location: https://synbiohub.org/public/igem/BBa_J07000/annotation1521549/range1521549/1
component: BBa_R0053

https://synbiohub.org/public/igem/BBa_J07000/annotation1521549/range1521549/1
type: Range
persistent identity: range1521549
display id: range1521549
version: 1
top level: BBa_J07000
direction: direction/forward
start: 1
end: 54
orientation: inline

Homol.
type: ComponentDefinition
persistent identity: BBa I13850
display id: BBa_I13850
version: 1
was generated by: iGEM to SBOL conversion
description: E coli Downstream Homologous Recombination Sequence
created: 2004-07-22T11:00:00Z
modified: 2015-08-31T04:07:36Z
owned by: james
owned by: myers
mutable description: This is the downstream sequence of the homologous recombination site for the E coli chromosome provided by Chris Farrell. The recombination site is in between the ybhH and ybhD genes on the E coli chromosome.
discontinued: false
dominant: false
experience: experience/None
group u list: _6_
m user id: 0
owner id: 159
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: robertb
type: DnaRegion
role: partType/Other
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_I13850_sequence/1

BBa_J07029
type: ComponentDefinition
persistent identity: BBa J07029
display id: BBa_J07029
version: 1
was generated by: iGEM to SBOL conversion
description: Antirabbit IgG scFV
created: 2005-07-17T11:00:00Z
modified: 2015-08-31T04:08:19Z
mutable provenance: Shen, et al. "Single-chain Fragment Variable Antibody Piezoimmunosensors" Analytical Chemistry, Vol. 77, No. 3, February 1, 2005.
owned by: james
owned by: myers
mutable description: Single chain fragment variable produced from the genetic information of an A10B hybridoma cell line. This scFV binds to the constant heavy chain 1 domain of rabbit IgG.
discontinued: false
dominant: false
experience: experience/None
group u list: _21_
m user id: 0
owner id: 355
owning group id: 21
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: maxiney
type: DnaRegion
role: partType/Other
role: SO:0000110
sequence annotation: XbaI restriction site
sequence annotation: Variable light chain region
sequence annotation: SpeI restriction site
sequence annotation: Variable heavy chain region
sequence annotation: Linker sequence
sequence: https://synbiohub.org/public/igem/BBa_J07029_sequence/1

XbaI restriction site
type: SequenceAnnotation
persistent identity: annotation1585901
display id: annotation1585901
version: 1
top level: BBa_J07029
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J07029/annotation1585901/range1585901/1

https://synbiohub.org/public/igem/BBa_J07029/annotation1585901/range1585901/1
type: Range
persistent identity: range1585901
display id: range1585901
version: 1
top level: BBa_J07029
direction: direction/forward
start: 1
end: 6
orientation: inline

Variable light chain region
type: SequenceAnnotation
persistent identity: annotation1585912
display id: annotation1585912
version: 1
top level: BBa_J07029
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J07029/annotation1585912/range1585912/1

https://synbiohub.org/public/igem/BBa_J07029/annotation1585912/range1585912/1
type: Range
persistent identity: range1585912
display id: range1585912
version: 1
top level: BBa_J07029
direction: direction/forward
start: 415
end: 744
orientation: inline

SpeI restriction site
type: SequenceAnnotation
persistent identity: annotation1585902
display id: annotation1585902
version: 1
top level: BBa_J07029
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J07029/annotation1585902/range1585902/1

https://synbiohub.org/public/igem/BBa_J07029/annotation1585902/range1585902/1
type: Range
persistent identity: range1585902
display id: range1585902
version: 1
top level: BBa_J07029
direction: direction/forward
start: 745
end: 750
orientation: inline

Variable heavy chain region
type: SequenceAnnotation
persistent identity: annotation1585911
display id: annotation1585911
version: 1
top level: BBa_J07029
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J07029/annotation1585911/range1585911/1

https://synbiohub.org/public/igem/BBa_J07029/annotation1585911/range1585911/1
type: Range
persistent identity: range1585911
display id: range1585911
version: 1
top level: BBa_J07029
direction: direction/forward
start: 7
end: 369
orientation: inline

Linker sequence
type: SequenceAnnotation
persistent identity: annotation1585910
display id: annotation1585910
version: 1
top level: BBa_J07029
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J07029/annotation1585910/range1585910/1

https://synbiohub.org/public/igem/BBa_J07029/annotation1585910/range1585910/1
type: Range
persistent identity: range1585910
display id: range1585910
version: 1
top level: BBa_J07029
direction: direction/forward
start: 370
end: 414
orientation: inline

https://synbiohub.org/public/igem/BBa_Y1005_sequence/1
type: Sequence
persistent identity: BBa Y1005 sequence
display id: BBa_Y1005_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: myers
owned by: james
elements: aacgaagcatctgtgcttcattttgtagaacaaaaatgcaacgcgagagcgctaatttttcaaacaaagaatctgagctgcatttttacagaacagaaatgcaacgcgaaagcgctattttaccaacgaagaatctgtgcttcatttttgtaaaacaaaaatgcaacgcgagagcgctaatttttcaaacaaagaatctgagctgcatttttacagaacagaaatgcaacgcgagagcgctattttaccaacaaagaatctatacttcttttttgttctacaaaaatgcatcccgagagcgctatttttctaacaaagcatcttagattactttttttctcctttgtgcgctctataatgcagtctcttgataactttttgcactgtaggtccgttaaggttagaagaaggctactttggtgtctattttctcttccataaaaaaagcctgactccacttcccgcgtttactgattactagcgaagctgcgggtgcattttttcaagataaaggcatccccgattatattctataccgatgtggattgcgcatactttgtgaacagaaagtgatagcgttgatgattcttcattggtcagaaaattatgaacggtttcttctattttgtctctatatactacgtataggaaatgtttacattttcgtattgttttcgattcactctatgaatagttcttactacaatttttttgtctaaagagtaatactagagataaacataaaaaatgtagaggtcgagtttagatgcaagttcaaggagcgaaaggtggatgggtaggttatatagggatatagcacagagatatatagcaaagagatacttttgagcaatgtttgtggaagcggtattcgcaatattttagtagctcgttacagtccggtgcgtttttggttttttgaaagtgcgtcttcagagcgcttttggttttcaaaagcgctctgaagttcctatactttctagagaataggaacttcggaataggaacttcaaagcgtttccgaaaacgagcgcttccgaaaatgcaacgcgagctgcgcacatacagctcactgttcacgtcgcacctatatctgcgtgttgcctgtatatatatatacatgagaagaacggcatagtgcgtgtttatgcttaaatgcgtacttatatgcgtctatttatgtaggatgaaaggtagtctagtacctcctgtgatattatcccattccatgcggggtatcgtatgcttccttcagcactaccctttagctgttctatatgctgccactcctcaattggattagtctcatccttcaatgctatcatttcctttgatattggatcata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0051_sequence/1
type: Sequence
persistent identity: BBa R0051 sequence
display id: BBa_R0051_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: taacaccgtgcgtgttgactattttacctctggcggtgataatggttgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J07001_sequence/1
type: Sequence
persistent identity: BBa J07001 sequence
display id: BBa_J07001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aataaacttgactaaagattcctttagtagataatttaagtgttctttaatttctactagagaaagaggagaaatactagatggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcaggctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagaattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagagaaagaggagaaatactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaag…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_Z0251_sequence/1
type: Sequence
persistent identity: BBa Z0251 sequence
display id: BBa_Z0251_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gaaattaatacgactcactatagggagaccacaac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_Y1011_sequence/1
type: Sequence
persistent identity: BBa Y1011 sequence
display id: BBa_Y1011_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gacacatataccaacgactacatctccacaattggagtggacttcaagattaagactgtagaactggacggcaagactgtaaagctacagatttgggacactgcaggtcaagaacgtttccgtactatcacttcatcttactaccgtggttcgcatgggatcattatcgtgtatgatgtcactgaccaagaatccttcaacggcgtgaagatgtggctgcaagagattgatcggtatgcaacctcaacagtgttgaagctattggtaggtaacaagtgtgatttaaaggacaagcgtgtcgtggaatatgacgtggcaaaggaatttgcggacgcgaataagatgccgttcttagagactagtgctttggactccaccaacgtcgaggatgcgtttttaaccatggctagacaaatcaaggaaagtatgtcccaacaaaacctgaacgaaaccactcagaagaaggaagacaaagggaacgtcaacctgaagggacagagtttaaccaacaccggtgggggctgctgttgaacaagcgcgcctctaccttgcagacccatataatataataactaaataagtaaataagacacacgcgagaacatatatacacaattacagtaacaataacaagaggacagatactaccaaaatgtgtggggaagcgggtaagctgccacagcaattaatgcacaacatttaacctacattcttccttatcggatcctcaaaacccttaaaaacatatgcctcaccctaacatattttccaattaaccctcaatatttctctgtcacccggcctctattttccattttcttctttacccgccacgcgtttttttctttcaaatttttttcttccttcttctttttcttccacgtcctcttgcataaataaataaaccgttttgaaaccaaactcgcctctctctctcctttttgaaatatttttgggtttgtttgatcctttccttcccaatctctcttgtttaatatatattcatttatatcacgctctctttttatcttcctttttttcctctctcttgtattcttccttcccctttctactcaaaccaagaagaaaaagaaaaggtcaatctttgttaaagaataggatcttctactacatcagcttttagatttttcacgcttactgcttttttcttcccaagatcgaaaatttactgaattaac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J07006_sequence/1
type: Sequence
persistent identity: BBa J07006 sequence
display id: BBa_J07006_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaaaataaaaacaggtgcacgcatcctcgcattatccgcattaacgacgatgatgttttccgcctcggctctcgccaaaatcgaagaaggtaaactggtaatctggattaacggcgataaaggctataacggtctcgctgaagtcggtaagaaattcgagaaagataccggaattaaagtcaccgttgagcatccggataaactggaagagaaattcccacaggttgcggcaactggcgatggccctgacattatcttctgggcacacgaccgctttggtggctacgctcaatctggcctgttggctgaaatcaccccggacaaagcgttccaggacaagctgtatccgtttacctgggatgccgtacgttacaacggcaagctgattgcttacccgatcgctgttgaagcgttatcgctgatttataacaaagatctgctgccgaacccgccaaaaacctgggaagagatcccggcgctggataaagaactgaaagcgaaaggtaagagcgcgctgatgttcaacctgcaagaaccgtacttcacctggccgctgattgctgctgacgggggttatgcgttcaagtatgaaaacggcaagtacgacattaaagacgtgggcgtggataacgctggcgcgaaagcgggtctgaccttcctggttgacctgattaaaaacaaacacatgaatgcagacaccgattactccatcgcagaagctgcctttaataaaggcgaaacagcgatgaccatcaacggcccgtgggcatggtccaacatcgacaccagcaaagtgaattatggtgtaacggtactgccgaccttcaagggtcaaccatccaaaccgttcgttggcgtgctgagcgcaggtattaacgccgccagtccgaacaaagagctggcgaaagagttcctcgaaaactatctgctgactgatgaaggtctggaagcggttaataaagacaaaccgctgggtgccgtagcgctgaagtcttacgaggaagagttggcgaaagatccacgtattgccgccaccatggaaaacgcccagaaaggtgaaatcatgccgaacatcccgcagatgtccgctttctggtatgccgtgcgtactgcggtgatcaacgccgccagcggtcgtcagactgtcgatgaagccctgaaagacgcgcagactcgtatcaccaagtaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I14070_sequence/1
type: Sequence
persistent identity: BBa I14070 sequence
display id: BBa_I14070_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gctgcaaacgacgaaaactacgctttagtagcttaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I11043_sequence/1
type: Sequence
persistent identity: BBa I11043 sequence
display id: BBa_I11043_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aggtcactaatactatctaagtagttgattcatagtgactggatatgttgcgttttgtcgcattatgtagtctatcatttaaccacagattagtgtaatgcgatgatttttaagtgattaatgttattttgtcatcctttaggtgaataagttgtatatttaaaatctctttaattatcagtaaattaatgtaagtaggtcattattagtcaaaataaaatcatttgtc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I11032_sequence/1
type: Sequence
persistent identity: BBa I11032 sequence
display id: BBa_I11032_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: acgaccttcgcattacgaatgcgctgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E0030_sequence/1
type: Sequence
persistent identity: BBa E0030 sequence
display id: BBa_E0030_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccttcggctacggcctgcaatgcttcgcccgctaccccgaccacatgaagctgcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagctaccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagtaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_Y1003_sequence/1
type: Sequence
persistent identity: BBa Y1003 sequence
display id: BBa_Y1003_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: agattgtactgagagtgcaccatagggttaattaaggcgcgccagatctgtttagcttgcctcgtccccgccgggtcacccggccagcgacatggaggcccagaataccctccttgacagtcttgacgtgcgcagctcaggggcatgatgtgactgtcgcccgtacatttagcccatacatccccatgtataatcatttgcatccatacattttgatggccgcacggcgcgaagcaaaaattacggctcctcgctgcagacctgcgagcagggaaacgctcccctcacagacgcgttgaattgtccccacgccgcgcccctgtagagaaatataaaaggttaggatttgccactgaggttcttctttcatatacttccttttaaaatcttgctaggatacagttctcacatcacatccgaacataaacaaccatgggtaaggaaaagactcacgtttcgaggccgcgattaaattccaacatggatgctgatttatatgggtataaatgggctcgcgataatgtcgggcaatcaggtgcgacaatctatcgattgtatgggaagcccgatgcgccagagttgtttctgaaacatggcaaaggtagcgttgccaatgatgttacagatgagatggtcagactaaactggctgacggaatttatgcctcttccgaccatcaagcattttatccgtactcctgatgatgcatggttactcaccactgcgatccccggcaaaacagcattccaggtattagaagaatatcctgattcaggtgaaaatattgttgatgcgctggcagtgttcctgcgccggttgcattcgattcctgtttgtaattgtccttttaacagcgatcgcgtatttcgtctcgctcaggcgcaatcacgaatgaataacggtttggttgatgcgagtgattttgatgacgagcgtaatggctggcctgttgaacaagtctggaaagaaatgcataagcttttgccattctcaccggattcagtcgtcactcatggtgatttctcacttgataaccttatttttgacgaggggaaattaataggttgtattgatgttggacgagtcggaatcgcagaccgataccaggatcttgccatcctatggaactgcctcggtgagttttctccttcattacagaaacggctttttcaaaaatatggtattgataatcctgatatgaataaattgcagtttcatttgatgctcgatgagtttttctaatcagtactgacaataaaaagattcttgttttcaagaacttgtcatttgtatagtttttttatattgtagttgttctattttaatcaaatgttagcgtgatttatattttttttcgcctcgacatcatctgcccagatgcgaagttaagtgcgcagaaagtaatatcatgcgtcaatcgtatgtgaatgctggtcgctatactgctgtcgattcgatactaacgccgccatccagtgtcgaaaacgagctcgaattcatcgatgattatgcggtgtgaaataccgcacag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I13851_sequence/1
type: Sequence
persistent identity: BBa I13851 sequence
display id: BBa_I13851_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cctttttcctgcttttgttaacgtaaccttgcgagttgtacgctcaaatagttgaacatgtaaatcttcctccatcttttttattcttcgcgttaatgcaggttgcgtaatattgagtaatttagcggcattgttaaacgaactggactctgccagtatgacaaatgccttcatacttgataattcatgcttcattattactccggaaaatggaagcgacgattttgggtggctggccgttaaaaattttaactgcatttagccaacttaaattaatgaaaaaatgttattaatcgttga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I13850_sequence/1
type: Sequence
persistent identity: BBa I13850 sequence
display id: BBa_I13850_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: agatgctttgcccttaatgtaaccatatcgcaataagttatgtttttaaattgagggcattattatgaaaaaaataccctgcgtgatgatgcgaggtggaacctcgaggggcgcgttcctgttagcggaacatttacccgaagatcaaacgcagcgcgataaaatattgatggcaattatgggttccggtaacgatctggaaattgacggtattggcggcggtaatccactgaccagtaaagtcgccattattagcc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_Y1010_sequence/1
type: Sequence
persistent identity: BBa Y1010 sequence
display id: BBa_Y1010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tagtacggattagaagccgccgagcgggtgacagccctccgaaggaagactctcctccgtgcgtcctcgtcttcaccggtcgcgttcctgaaacgcagatgtgcctcgcgccgcactgctccgaacaataaagattctacaatactagcttttatggttatgaagaggaaaaattggcagtaacctggccccacaaaccttcaaatgaacgaatcaaattaacaaccataggatgataatgcgattagttttttagccttatttctggggtaattaatcagcgaagcgatgatttttgatctattaacagatatataaatgcaaaaactgcataaccactttaactaatactttcaacattttcggtttgtattacttcttattcaaatgtaataaaagtatcaacaaaaaattgttaatatacctctatactttaacgtcaaggagaaaaaaccccggat
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J07000_sequence/1
type: Sequence
persistent identity: BBa J07000 sequence
display id: BBa_J07000_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aataaacttgactaaagattcctttagtagataatttaagtgttctttaatttctactagagaaagaggagaaatactagatggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcaggctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagaattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagagaaagaggagaaatactagatgagcacaaaaaagaaaccattaacacaagagcagcttgaggacgcacgtcgccttaaagcaatttatgaaaaaaagaaaaatgaacttggcttatcccaggaatctgtcgcagacaagatggggatggggcagtcaggcgttggtgctttatttaatggcatcaatgcattaaatgcttataacgccgcattgcttgcaaaaattctcaaagttagcgttgaagaatttagcccttcaatcgccagagaaatctacgagatgtatgaagcggttagtatgcagccgtcacttagaagtgagtatgagtaccctgttttttctcatgttcaggcagggatgttctcacctgagcttagaacctttaccaaaggtgatgcggagagatgggtaagcacaaccaaaaaagccagtgattctgcattctggcttgaggttgaaggtaattccatgaccgcaccaacaggctccaagccaagctttcctgacggaatgttaattctcgttgaccctgagcaggctgttgagccaggtgatttct…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_Y1014_sequence/1
type: Sequence
persistent identity: BBa Y1014 sequence
display id: BBa_Y1014_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: agtttatcattatcaatactcgccatttcaaagaatacgtaaataattaatagtagtgattttcctaactttatttagtcaaaaaattagccttttaattctgctgtaacccgtacatgcccaaaatagggggcgggttacacagaatatataacatcgtaggtgtctgggtgaacagtttattcctggcatccactaaatataatggagcccgctttttaagctggcatccagaaaaaaaaagaatcccagcaccaaaatattgttttcttcaccaaccatcagttcataggtccattctcttagcgcaactacagagaacaggggcacaaacaggcaaaaaacgggcacaacctcaatggagtgatgcaacctgcctggagtaaatgatgacacaaggcaattgacccacgcatgtatctatctcattttcttacaccttctattaccttctgctctctctgatttggaaaaagctgaaaaaaaaggttgaaaccagttccctgaaattattcccctacttgactaataagtatataaagacggtaggtattgattgtaattctgtaaatctatttcttaaacttcttaaattctacttttatagttagtcttttttttagttttaaaacaccagaacttagtttcgacggat
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0051_sequence/1
type: Sequence
persistent identity: BBa C0051 sequence
display id: BBa_C0051_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgagcacaaaaaagaaaccattaacacaagagcagcttgaggacgcacgtcgccttaaagcaatttatgaaaaaaagaaaaatgaacttggcttatcccaggaatctgtcgcagacaagatggggatggggcagtcaggcgttggtgctttatttaatggcatcaatgcattaaatgcttataacgccgcattgcttgcaaaaattctcaaagttagcgttgaagaatttagcccttcaatcgccagagaaatctacgagatgtatgaagcggttagtatgcagccgtcacttagaagtgagtatgagtaccctgttttttctcatgttcaggcagggatgttctcacctgagcttagaacctttaccaaaggtgatgcggagagatgggtaagcacaaccaaaaaagccagtgattctgcattctggcttgaggttgaaggtaattccatgaccgcaccaacaggctccaagccaagctttcctgacggaatgttaattctcgttgaccctgagcaggctgttgagccaggtgatttctgcatagccagacttgggggtgatgagtttaccttcaagaaactgatcagggatagcggtcaggtgtttttacaaccactaaacccacagtacccaatgatcccatgcaatgagagttgttccgttgtggggaaagttatcgctagtcagtggcctgaagagacgtttggcgctgcaaacgacgaaaactacgctttagtagcttaataacgctgatagtgctagtgtagatcgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0040_sequence/1
type: Sequence
persistent identity: BBa B0040 sequence
display id: BBa_B0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aggttctgttaagtaactgaacccaatgtcgttagtgacgcttacctcttaagaggtcactgacctaaca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J07003_sequence/1
type: Sequence
persistent identity: BBa J07003 sequence
display id: BBa_J07003_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gaaattaatacgactcactatagggagaccacaactactagagaaagaggagaaatactagatggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcaggctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagaattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagagaaagaggagaaatactagatgagcacaaaaaagaaaccattaacacaagagcagcttgaggacgcacgtcgccttaaagcaatttatgaaaaaaagaaaaatgaacttggcttatcccaggaatctgtcgcagacaagatggggatggggcagtcaggcgttggtgctttatttaatggcatcaatgcattaaatgcttataacgccgcattgcttgcaaaaattctcaaagttagcgttgaagaatttagcccttcaatcgccagagaaatctacgagatgtatgaagcggttagtatgcagccgtcacttagaagtgagtatgagtaccctgttttttctcatgttcaggcagggatgttctcacctgagcttagaacctttaccaaaggtgatgcggagagatgggtaagcacaaccaaaaaagccagtgattctgcattctggcttgaggttgaaggtaattccatgaccgcaccaacaggctccaagccaagctttcctgacggaatgttaattctcgttgaccctgagcaggctgttgagccaggtgatttctgcatagccagacttggggg…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_Y1004_sequence/1
type: Sequence
persistent identity: BBa Y1004 sequence
display id: BBa_Y1004_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: catcacgtgctataaaaataattataatttaaattttttaatataaatatataaattaaaaatagaaagtaaaaaaagaaattaaagaaaaaatagtttttgttttccgaagatgtaaaagactctagggggatcgccaacaaatactaccttttatcttgctcttcctgctctcaggtattaatgccgaattgtttcatcttgtctgtgtagaagaccacacacgaaaatcctgtgattttacattttacttatcgttaatcgaatgtatatctatttaatctgcttttcttgtctaataaatatatatgtaaagtacgctttttgttgaaattttttaaacctttgtttatttttttttcttcattccgtaactcttctaccttctttatttactttctaaaatccaaatacaaaacataaaaataaataaacacagagtaaattcccaaattattccatcattaaaagatacgaggcgcgtgtaagttacaggcaagcgatc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0012_sequence/1
type: Sequence
persistent identity: BBa C0012 sequence
display id: BBa_C0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcaggctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J07004_sequence/1
type: Sequence
persistent identity: BBa J07004 sequence
display id: BBa_J07004_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gaaattaatacgactcactatagggagaccacaactactagagaaagaggagaaatactagatggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcaggctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagaattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagagaaagaggagaaatactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttctt…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0011_sequence/1
type: Sequence
persistent identity: BBa R0011 sequence
display id: BBa_R0011_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcaca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_Y1008_sequence/1
type: Sequence
persistent identity: BBa Y1008 sequence
display id: BBa_Y1008_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgtctgcccctaagaagatcgtcgttttgccaggtgaccacgttggtcaagaaatcacagccgaagccattaaggttcttaaagctatttctgatgttcgttccaatgtcaagttcgatttcgaaaatcatttaattggtggtgctgctatcgatgctacaggtgtcccacttccagatgaggcgctggaagcctccaagaaggttgatgccgttttgttaggtgctgtgggtggtcctaaatggggtaccggtagtgttagacctgaacaaggtttactaaaaatccgtaaagaacttcaattgtacgccaacttaagaccatgtaactttgcatccgactctcttttagacttatctccaatcaagccacaatttgctaaaggtactgacttcgttgttgtcagagaattagtgggaggtatttactttggtaagagaaaggaagacgatggtgatggtgtcgcttgggatagtgaacaatacaccgttccagaagtgcaaagaatcacaagaatggccgctttcatggccctacaacatgagccaccattgcctatttggtccttggataaagctaatgttttggcctcttcaagattatggagaaaaactgtggaggaaaccatcaagaacgaattccctacattgaaggttcaacatcaattgattgattctgccgccatgatcctagttaagaacccaacccacctaaatggtattataatcaccagcaacatgtttggtgatatcatctccgatgaagcctccgttatcccaggttccttgggtttgttgccatctgcgtccttggcctctttgccagacaagaacaccgcatttggtttgtacgaaccatgccacggttctgctccagatttgccaaagaataaggttgaccctatcgccactatcttgtctgctgcaatgatgttgaaattgtcattgaacttgcctgaagaaggtaaggccattgaagatgcagttaaaaaggttttggatgcaggtatcagaactggtgatttaggtggttccaacagtaccaccgaagtcggtgatgctgtcgccgaagaagttaagaaaatccttgcttaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K361001_sequence/1
type: Sequence
persistent identity: BBa K361001 sequence
display id: BBa_K361001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gggcccagatctagaaagatggcaatactttcgtgccaatccggtacgtggtttgtcttacaatcgttgtttgagtgtaacaatcgatctttcattgactcgcacaatgtatcggacaatcgatccttgtctcttacaatgtatcggtctgtctttgtatccatctttgacacaatcattgagacaatcactgactcggtgtatccatctttgactgagacaatcggtgacacaatgaatctttgactccttgagtccaacaatgtatcggtctgtctttgtatccatctttgacacaatcattgagacaatgtatcggtcggtcgatgagctgctggcctacgtgccaatccggtacgtggagtgagaataagagagagagagaaagacagcaagtgagtaagctagcaagatagactaatagtcggatccctcgag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I14071_sequence/1
type: Sequence
persistent identity: BBa I14071 sequence
display id: BBa_I14071_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gctgcaaacgacgaaaactacgctttagtagcttaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J07029_sequence/1
type: Sequence
persistent identity: BBa J07029 sequence
display id: BBa_J07029_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tctagaatggcccaggtgcagctgcagcagtcaggaactgaagtggtaaagcctggggcttcagtgaagttgtcctgcaaggcttctggctacatcttcacaagttatgatatagactgggtgaggcagacgcctgaacagggacttgagtggattggatggatttttcctggagaggggagtactgaatacaatgagaagttcaagggcagggccacactgagtgtagacaagtcctccagcacagcctatatggagctcactaggctgacatctgaggactctgctgtctatttctgtgctagaggggactactataggcgctactttgacttgtggggccaagggaccacggtcaccgtctcctcaggtggaggcggttcaggcggaggtggctctggcggtggcggatcggacattgagctcacccagtctccaacaatcatgtctgcatctccaggggagagggtcaccatgacctgcagtgccagctcaagtatacgttacatatattggtaccaacagaagcctggatcctcccccagactcctgatttatgacacatccaacgtggcttctggagtcccttctcgcttcagtggcagtgggtctgggacctcttattctctcacaatcaaccgaatggaggctgaggatgctgccacttattactgccaggagtggagtggttatccgtacacgttcggaggggggaccaagctggagctgaaacaggcggccgcaactagt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_Y1016_sequence/1
type: Sequence
persistent identity: BBa Y1016 sequence
display id: BBa_Y1016_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tgagatcctttttttctgcgcgtaatctgctgcttgcaaacaaaaaaaccaccgctaccagcggtggtttgtttgccggatcaagagctaccaactctttttccgaaggtaactggcttcagcagagcgcagataccaaatactgtccttctagtgtagccgtagttaggccaccacttcaagaactctgtagcaccgcctacatacctcgctctgctaatcctgttaccagtggctgctgccagtggcgataagtcgtgtcttaccgggttggactcaagacgatagttaccggataaggcgcagcggtcgggctgaacggggggttcgtgcacacagcccagcttggagcgaacgacctacaccgaactgagatacctacagcgtgagctatgagaaagcgccacgcttcccgaagggagaaaggcggacaggtatccggtaagcggcagggtcggaacaggagagcgcacgagggagcttccagggggaaacgcctggtatctttatagtcctgtcgggtttcgccacctctgacttgagcgtcgatttttgtgatgctcgtcaggggggcggagcctatggaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I11042_sequence/1
type: Sequence
persistent identity: BBa I11042 sequence
display id: BBa_I11042_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: attccgctttgcgactcaacctttttcacctaaagtgcaccgaccgtgaat
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I11022_sequence/1
type: Sequence
persistent identity: BBa I11022 sequence
display id: BBa_I11022_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: accactttgtacaagaaagctgggt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_Y1009_sequence/1
type: Sequence
persistent identity: BBa Y1009 sequence
display id: BBa_Y1009_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgtctgttattaatttcacaggtagttctggtccattggtgaaagtttgcggcttgcagagcacagaggccgcagaatgtgctctagattccgatgctgacttgctgggtattatatgtgtgcccaatagaaagagaacaattgacccggttattgcaaggaaaatttcaagtcttgtaaaagcatataaaaatagttcaggcactccgaaatacttggttggcgtgtttcgtaatcaacctaaggaggatgttttggctctggtcaatgattacggcattgatatcgtccaactgcatggagatgagtcgtggcaagaataccaagagttcctcggtttgccagttattaaaagactcgtatttccaaaagactgcaacatactactcagtgcagcttcacagaaacctcattcgtttattcccttgtttgattcagaagcaggtgggacaggtgaacttttggattggaactcgatttctgactgggttggaaggcaagagagccccgaaagcttacattttatgttagctggtggactgacgccagaaaatgttggtgatgcgcttagattaaatggcgttattggtgttgatgtaagcggaggtgtggagacaaatggtgtaaaagactctaacaaaatagcaaatttcgtcaaaaatgctaagaaatag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K629005_sequence/1
type: Sequence
persistent identity: BBa K629005 sequence
display id: BBa_K629005_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgagcactgataataagcaatcattgcccgcgattaccctcgcggcgattggagttgtctacggcgatattggtaccagcccgttatatacacttcgtgaatgtttgtccggccagtttggttttggcgttgaacgcgatgccgtgtttggctttttatcgctgatcttctggctgctaatctttgtggtttccattaaatatctcaccttcgtgatgcgggcagataacgccggtgagggggggatcctgacgttgatgtcgcttgccgggcgtaatacgtcggcgcgaaccacatcaatgctggtgattatggggctaatcggcggcagctttttctatggtgaagtcgtcataacacccgctatttcggtgatgtcagccattgaaggtctggaaatcgtcgccccgcagctggatacctggatagttcccctctcaattatcgttctcactttattatttatgattcaaaagcatggcacggctatggtcggtaagctgtttgcgccgatcatgctgacctggtttttgattctggcaggactggggttacgtagcattattgctaacccggaagtgctgcatgcgctgaatccgatgtgggcggtgcatttcttccttgaatacaaaacggtttcttttattgcattaggggcagtggtgctgtcgattacgggggtcgaggcgctgtatgctgatatgggacactttggtaagttccctattcgtctggcgtggttcactgtcgtattaccttccttaactcttaattacttcggccagggagcgctgttgttaaagaacccggaagcgattaagaacccgttcttccttttggcaccggactgggcgctgatcccgctgctgatcatcgccgcactggcgacggtaatcgcctcgcaggcggttatctctggcgtcttttcattgacgcgtcaggcggtacgtctgggatatttgtcgccgatgcgcattattcacacctccgaaatggagtcagggcaaatctatattccgtttgtgaactggatgctctatgtcgcggtcgtgattgtgattgtcagctttgagcactccagcaacctggcggcggcgtacgggattgcggtgaccggaaccatggtgctgacgtctattctctcgactaccgtggcacgtcagaactggcactggaataagtattttgttgcgctgatcctgattgctttcctttgtgtcgatattccattgttcaccgctaacctcgataaactgctctccggcggctggttgccattgagcctcggtactgtgatgtttatcgtgatgaccacctggaagagcgagcgtttccgcttgctgcggcggatgcatgaacatggtaactctctggaagcgatgattgcttcgctggagaaatcaccgcctgttcgcgtgcccgggaccgcggtgtatatgtcgcgtgcaatcaacgtcattccctttgcgctgatgcataaccttaaacataacaaggtattgcatgagcgggtgattctgttaactctgcgcaccgaagacgctccatatgtccataacgtccgtcgggtacagattgaacaactgtcgcccactttctggcgcgtggtggcaagttatggttggcgagaaacgccaaacgtagaagaagttttccaccgctgcggtctggaaggattaagttgccggatgatggaaacctccttctttatgtcgcatgagtcgttgatcctcggcaaacgcccgtggtatttgcgtctgcgcggcaagctgtacttgctgctgcaacgtaatgcgctgcgtgcaccagatcaatttgaaatcccgccaaacagggttatcgaactgggtactcaggtcgaaatctaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I11023_sequence/1
type: Sequence
persistent identity: BBa I11023 sequence
display id: BBa_I11023_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tacaggtcactaataccatctaagtagttgattcatagtgactggatatgttgtgttttacagtattatgtagtctgttttttatgcaaaatctaatttaatatattgatatttatatcattttacgtttctcgttcagctttcttgtacaaagttggcattataagaaagcattgcttatcaatttgttgcaacgaacaggtcactatcagtcaaaataaaatcattattt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_Y1002_sequence/1
type: Sequence
persistent identity: BBa Y1002 sequence
display id: BBa_Y1002_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgagtattcaacatttccgtgtcgcccttattcccttttttgcggcattttgccttcctgtttttgctcacccagaaacgctggtgaaagtaaaagatgctgaagatcagttgggtgcacgagtgggttacatcgaactggatctcaacagcggtaagatccttgagagttttcgccccgaagaacgttttccaatgatgagcacttttaaagttctgctatgtggcgcggtattatcccgtattgacgccgggcaagagcaactcggtcgccgcatacactattctcagaatgacttggttgagtactcaccagtcacagaaaagcatcttacggatggcatgacagtaagagaattatgcagtgctgccataaccatgagtgataacactgcggccaacttacttctgacaacgatcggaggaccgaaggagctaaccgcttttttgcacaacatgggggatcatgtaactcgccttgatcgttgggaaccggagctgaatgaagccataccaaacgacgagcgtgacaccacgatgcctgtagcaatggcaacaacgttgcgcaaactattaactggcgaactacttactctagcttcccggcaacaattaatagactggatggaggcggataaagttgcaggaccacttctgcgctcggcccttccggctggctggtttattgctgataaatctggagccggtgagcgtgggtctcgcggtatcattgcagcactggggccagatggtaagccctcccgtatcgtagttatctacacgacggggagtcaggcaactatggatgaacgaaatagacagatcgctgagataggtgcctcactgattaagcattggtaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0053_sequence/1
type: Sequence
persistent identity: BBa R0053 sequence
display id: BBa_R0053_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aataaacttgactaaagattcctttagtagataatttaagtgttctttaatttc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_Y1006_sequence/1
type: Sequence
persistent identity: BBa Y1006 sequence
display id: BBa_Y1006_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgacagagcagaaagccctagtaaagcgtattacaaatgaaaccaagattcagattgcgatctctttaaagggtggtcccctagcgatagagcactcgatcttcccagaaaaagaggcagaagcagtagcagaacaggccacacaatcgcaagtgattaacgtccacacaggtatagggtttctggaccatatgatacatgctctggccaagcattccggctggtcgctaatcgttgagtgcattggtgacttacacatagacgaccatcacaccactgaagactgcgggattgctctcggtcaagcttttaaagaggccctactggcgcgtggagtaaaaaggtttggatcaggatttgcgcctttggatgaggcactttccagagcggtggtagatctttcgaacaggccgtacgcagttgtcgaacttggtttgcaaagggagaaagtaggagatctctcttgcgagatgatcccgcattttcttgaaagctttgcagaggctagcagaattaccctccacgttgattgtctgcgaggcaagaatgatcatcaccgtagtgagagtgcgttcaaggctcttgcggttgccataagagaagccacctcgcccaatggtaccaacgatgttccctccaccaaaggtgttcttatgtag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I1010_sequence/1
type: Sequence
persistent identity: BBa I1010 sequence
display id: BBa_I1010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcacgcgaaaaatcaataatcagacaacaagatgtgctcgacaaaaaagaaaccattaacacaagagcagcttgaggacgcacgtcgccttaaagcaatttatgaaaaaaagaaaaatgaacttggcttatcccaggaatctgtcgcagacaagatggggatggggcagtcaggcgttggtgctttatttaatggcatcaatgcattaaatgcttataacgccgcattgcttgcaaaaattctcaaagttagcgttgaagaatttagcccttcaatcgccagagaaatctacgagatgtatgaagcggttagtatgcagccgtcacttagaagtgagtatgagtaccctgttttttctcatgttcaggcagggatgttctcacctgagcttagaacctttaccaaaggtgatgcggagagatgggtaagcacaaccaaaaaagccagtgattctgcattctggcttgaggttgaaggtaattccatgaccgcaccaacaggctccaagccaagctttcctgacggaatgttaattctcgttgaccctgagcaggctgttgagccaggtgatttctgcatagccagacttgggggtgatgagtttaccttcaagaaactgatcagggatagcggtcaggtgtttttacaaccactaaacccacagtacccaatgatcccatgcaatgagagttgttccgttgtggggaaagttatcgctagtcagtggcctgaagagacgtttggcgctgcaaacgacgaaaactacgctttagtagcttaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E0020_sequence/1
type: Sequence
persistent identity: BBa E0020 sequence
display id: BBa_E0020_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtgaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccctgacctggggcgtgcagtgcttcagccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacatcagccacaacgtctatatcaccgccgacaagcagaagaacggcatcaaggccaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagcacccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagtaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_Y1007_sequence/1
type: Sequence
persistent identity: BBa Y1007 sequence
display id: BBa_Y1007_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgtcgaaagctacatataaggaacgtgctgctactcatcctagtcctgttgctgccaagctatttaatatcatgcacgaaaagcaaacaaacttgtgtgcttcattggatgttcgtaccaccaaggaattactggagttagttgaagcattaggtcccaaaatttgtttactaaaaacacatgtggatatcttgactgatttttccatggagggcacagttaagccgctaaaggcattatccgccaagtacaattttttactcttcgaagacagaaaatttgctgacattggtaatacagtcaaattgcagtactctgcgggtgtatacagaatagcagaatgggcagacattacgaatgcacacggtgtggtgggcccaggtattgttagcggtttgaagcaggcggcagaagaagtaacaaaggaacctagaggccttttgatgttagcagaattgtcatgcaagggctccctatctactggagaatatactaagggtactgttgacattgcgaagagcgacaaagattttgttatcggctttattgctcaaagagacatgggtggaagagatgaaggttacgattggttgattatgacacccggtgtgggtttagatgacaagggagacgcattgggtcaacagtatagaaccgtggatgatgtggtctctacaggatctgacattattattgttggaagaggactatttgcaaagggaagggatgctaaggtagagggtgaacgttacagaaaagcaggctgggaagcatatttgagaagatgcggccagcaaaactaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J07031_sequence/1
type: Sequence
persistent identity: BBa J07031 sequence
display id: BBa_J07031_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tctagaatggcccaggtgcagctgcaacagtcaggaactgaagtggtaaagcctggggcttcagtgaagttgtcctgcaaggcttctggctacatcttcacaagttatgatatagactgggtgaggcagacgcctgaacagggacttgagtggattggatggatttttcctggagaggggagtactgaatacaatgagaagttcaagggcagggccacactgagtgtagacaagtcctccagcacagcctatatggagctcactaggctgacatctgaggactctgctgtctatttctgtgctagaggggactactataggcgctactttgacttgtggggccaagggaccacggtcaccgtctcctcaggtggaggcggttcaggcggaggtggctctggcggtggcggatcggacattgagctcacccagtctccaacaatcatgtctgcatctccaggggagagggtcaccatgacctgtagtgccagctcaagtatacgttacatatattggtaccaacagaagcctggatcctcccccagactcctgatttatgacacatccaacgtggcttctggagtcccttctcgcttcagtggcagtgggtctgggacctcttattctctcacaatcaaccgaatggaggctgaggatgctgccacttattactgccaggagtggagtggttatccgtacacgttcggaggggggaccaagctggagctgaaacaggcggctgcataataaactagt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I11033_sequence/1
type: Sequence
persistent identity: BBa I11033 sequence
display id: BBa_I11033_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ctaagtggtttgggacaaaaatgggacatacaaatctttgcatcggtttgcaaggctttgcatgtctttcgaagatgggacgtgtgagcgcaggtatgacgtggtatgttgttgacttaaaaggtagttcttataattcgtaatgcgaaggtcgtaggttcgactcctattatcggcaccagttaaatcaaatacttacgtattattcgtgccttccttatttttactgtgggacatatttgggacagaagtaccaaaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J07002_sequence/1
type: Sequence
persistent identity: BBa J07002 sequence
display id: BBa_J07002_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatactagatggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcaggctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagaattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagagaaagaggagaaatactagatgagcacaaaaaagaaaccattaacacaagagcagcttgaggacgcacgtcgccttaaagcaatttatgaaaaaaagaaaaatgaacttggcttatcccaggaatctgtcgcagacaagatggggatggggcagtcaggcgttggtgctttatttaatggcatcaatgcattaaatgcttataacgccgcattgcttgcaaaaattctcaaagttagcgttgaagaatttagcccttcaatcgccagagaaatctacgagatgtatgaagcggttagtatgcagccgtcacttagaagtgagtatgagtaccctgttttttctcatgttcaggcagggatgttctcacctgagcttagaacctttaccaaaggtgatgcggagagatgggtaagcacaaccaaaaaagccagtgattctgcattctggcttgaggttgaaggtaattccatgaccgcaccaacaggctccaagccaagctttcctgacggaatgttaattctcgttgaccctgagcaggctgttgagccaggtgatttct…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I11053_sequence/1
type: Sequence
persistent identity: BBa I11053 sequence
display id: BBa_I11053_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaatctttccccaaaataaaaacgaacgtcaatgaaatcaaacggttgaataaagttgattttggctaatacaaagacaagaaaataatatttatgattaaatatcagcgagttgaatacataatttttatatactgctgcgccatatgggctggactgaagccgcagacctgattgttaaaggtatggaaggcgcaatcaacgccaagaccgtaacctatgacttcgaacgtctgatggaaggcgctaaactgctgaaatgcagcgagtttggtgacgcgatcatcaaaaatatgtaattaccacatgtgttaaatattataacgggcgtataacacgcccgttgttttatgatgatgtaaaatcttccccaaaactttccccaaaacccttccccaaaactggctattttctatgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0040_sequence/1
type: Sequence
persistent identity: BBa R0040 sequence
display id: BBa_R0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0040_sequence/1
type: Sequence
persistent identity: BBa C0040 sequence
display id: BBa_C0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_Y1012_sequence/1
type: Sequence
persistent identity: BBa Y1012 sequence
display id: BBa_Y1012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atcgttcatatacctcgtacaaatcaaatttgtctcttggaataccgaaaattagccgctcaaacactctccgttgttaatgacaattacaccatagccgctgccgtagaaaatgcacggtcttccgtgacgagaagattcgggggcgaatcgcatagatcgctctttctcctctttctcaattctgtcttatctgtatttataacatgagctcataaacaaagtccctgtttatatatattgatgacttatatatctttcgcttcagcttcttccctcatttctacttaatattatcttttgaaattagcgcgatcaattgcattcttttccacaccaccttcaaatttaagaattcatatttgaatcgaaccgtatgttttacctctcaataacttgtttcattaatagctccttccacaaccaccttcatcttctctgatttgaattattaccgacatgaatgttactctcctgaaaatgttaattatatcaacttaacaggccttgaaagtgaagtgttatagtttctcttcctttgtttttcccactttcttttttttatatatatcttatattggtttaagcacagcggattgctttcttctaatacaagaactagaacataggtccacccgtgtgatgttgcattctgaaatgtttagttatctctgtcagaacagcgaggtaacacttatttttgttacgaccaatccgtaattcaagcgtgggtattcatatgaccagagataataatacagcgaatactattgaaatcgtcccttttttgtttaggaagaacggacaaatcggtcgtctgctcgaaatgattagtagtgcgtcacccggatcagcaaaatgacacacacgaaatacgaggaaaaagtcggtcgaaaggggcaaatgttattataagtccctccagtagtttttttttttttcaaatattcatcatcaaaggttacgaaatcttttgagctatcttaaacattcgttctttttatcaaatttcaattactaacttattttttcaaaaaaaattgcctctcccggtttttaatcattatttttttcgattgattaagggggaaaagcaaagaacgagaaaacttggacagaaggttaatactctgacaatttcaaaacgaagtaaaaagaaaaattatcaaatcaacaaaaagtacccgttacaacaaaaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I11052_sequence/1
type: Sequence
persistent identity: BBa I11052 sequence
display id: BBa_I11052_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atatggcgcagcatcatct
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z