//classic/plasmid/uncategorized
type: Collection
persistent identity: classic plasmid uncategorized
display id: classic_plasmid_uncategorized
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
member: BBa_I11005
member: BBa_K283041
member: BBa_K394001
member: BBa_K319043
member: F plasmid
member: pSB1AK3-1
member: BBa_I11007
member: BBa_I11008
member: pSB1AC3-1
member: BBa_I11003
member: pSB1AT3-1
member: pSB2K0
member: BBa_I11004
member: BBa_K283042
member: BBa_K394002
member: BBa_I11006
member: BBa_J01077
member: BBa_I50010
member: pSB1A1

//classic/plasmid
type: Collection
persistent identity: classic plasmid
display id: classic_plasmid
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
member: //classic/plasmid/uncategorized
member: //classic/plasmid/measurement

//classic/plasmid/measurement
type: Collection
persistent identity: classic plasmid measurement
display id: classic_plasmid_measurement
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
member: BBa_K1937005
member: Screening
member: BBa_K1976000
member: BBa_K1976001

BBa_J01077
type: ComponentDefinition
persistent identity: BBa J01077
display id: BBa_J01077
version: 1
was generated by: iGEM to SBOL conversion
description: F plasmid (x-OriT, x-TraJ)
created: 2005-11-10T12:00:00Z
modified: 2015-08-31T04:08:12Z
owned by: myers
owned by: james
mutable description: F plasmid with OriT and TraJ knocked out by lambda-red
discontinued: false
dominant: false
experience: experience/Works
group u list: _13_
m user id: 0
owner id: 395
owning group id: 13
sample status: No part sequence
status: status/Planning
bookmark: false
star: false
creator: Golden Bear
type: DnaRegion
role: SO:0000155
role: partType/Plasmid

BBa_I11007
type: ComponentDefinition
persistent identity: BBa I11007
display id: BBa_I11007
version: 1
was generated by: iGEM to SBOL conversion
description: pZE2 PLtetO-1 plasmid--MCS2
created: 2004-08-17T11:00:00Z
modified: 2015-08-31T04:07:30Z
owned by: james
owned by: myers
mutable description: This plasmid contains the ColE1 origin of replication, kanamycin resistance marker, and tet0-1 promoter.
discontinued: false
dominant: false
experience: experience/None
group u list: _2_
m user id: 0
owner id: 102
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Lutz and Bujard
type: DnaRegion
role: SO:0000155
role: partType/Plasmid
sequence: https://synbiohub.org/public/igem/BBa_I11007_sequence/1

BBa_B0032
type: ComponentDefinition
persistent identity: BBa B0032
display id: BBa_B0032
version: 1
was generated by: iGEM to SBOL conversion
description: RBS.3 (medium) -- derivative of BBa_0030
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Weak1 RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0033</bb_part>.
discontinued: false
dominant: true
experience: experience/Works
group u list: _41_44_48_46_1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("RBS-2" in figure 4-14 of thesis). Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: BBa_B0032
sequence annotation: RBS
sequence annotation: RBS-3\Weak
sequence: https://synbiohub.org/public/igem/BBa_B0032_sequence/1

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation7027
display id: annotation7027
version: 1
top level: BBa_B0032
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_B0032/annotation7027/range7027/1

https://synbiohub.org/public/igem/BBa_B0032/annotation7027/range7027/1
type: Range
persistent identity: range7027
display id: range7027
version: 1
top level: BBa_B0032
direction: direction/forward
start: 1
end: 13
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation1710
display id: annotation1710
version: 1
top level: BBa_B0032
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_B0032/annotation1710/range1710/1

https://synbiohub.org/public/igem/BBa_B0032/annotation1710/range1710/1
type: Range
persistent identity: range1710
display id: range1710
version: 1
top level: BBa_B0032
direction: direction/forward
start: 7
end: 10
orientation: inline

RBS-3\Weak
type: SequenceAnnotation
persistent identity: annotation1709
display id: annotation1709
version: 1
top level: BBa_B0032
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_B0032/annotation1709/range1709/1

https://synbiohub.org/public/igem/BBa_B0032/annotation1709/range1709/1
type: Range
persistent identity: range1709
display id: range1709
version: 1
top level: BBa_B0032
direction: direction/forward
start: 1
end: 13
orientation: inline

BBa_K283041
type: ComponentDefinition
persistent identity: BBa K283041
display id: BBa_K283041
version: 1
was generated by: iGEM to SBOL conversion
description: constitutive promoter-rbs-rhlR
created: 2009-10-18T11:00:00Z
modified: 2015-05-08T01:11:48Z
mutable provenance: From the registry
owned by: james
owned by: myers
mutable description: The rhlR coding sequence on vector J61002
discontinued: false
dominant: false
experience: experience/None
group u list: _383_
m user id: 0
owner id: 5494
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: BBa_K283041
star: false
creator: Nelson Chu, W Ling , Shi Lei
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_J23100
component: BBa_C0071
component: BBa_B0034
sequence annotation: BBa_C0071
sequence annotation: BBa_J23100
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K283041_sequence/1

BBa_J23100
type: Component
persistent identity: component2246835
display id: component2246835
version: 1
top level: BBa_K283041
access: public
definition: BBa_J23100

BBa_C0071
type: Component
persistent identity: component2246841
display id: component2246841
version: 1
top level: BBa_K283041
access: public
definition: rhlR

BBa_B0034
type: Component
persistent identity: component2246837
display id: component2246837
version: 1
top level: BBa_K283041
access: public
definition: BBa_B0034

BBa_C0071
type: SequenceAnnotation
persistent identity: annotation2246841
display id: annotation2246841
version: 1
top level: BBa_K283041
location: https://synbiohub.org/public/igem/BBa_K283041/annotation2246841/range2246841/1
component: BBa_C0071

https://synbiohub.org/public/igem/BBa_K283041/annotation2246841/range2246841/1
type: Range
persistent identity: range2246841
display id: range2246841
version: 1
top level: BBa_K283041
direction: direction/reverse
start: 62
end: 848
orientation: inline

BBa_J23100
type: SequenceAnnotation
persistent identity: annotation2246835
display id: annotation2246835
version: 1
top level: BBa_K283041
location: https://synbiohub.org/public/igem/BBa_K283041/annotation2246835/range2246835/1
component: BBa_J23100

https://synbiohub.org/public/igem/BBa_K283041/annotation2246835/range2246835/1
type: Range
persistent identity: range2246835
display id: range2246835
version: 1
top level: BBa_K283041
direction: direction/reverse
start: 1
end: 35
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2246837
display id: annotation2246837
version: 1
top level: BBa_K283041
location: https://synbiohub.org/public/igem/BBa_K283041/annotation2246837/range2246837/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K283041/annotation2246837/range2246837/1
type: Range
persistent identity: range2246837
display id: range2246837
version: 1
top level: BBa_K283041
direction: direction/reverse
start: 44
end: 55
orientation: inline

BBa_K1976005
type: ComponentDefinition
persistent identity: BBa K1976005
display id: BBa_K1976005
version: 1
was generated by: iGEM to SBOL conversion
description: GFP with LVA-Tag
created: 2016-10-13T11:00:00Z
modified: 2016-10-18T01:53:18Z
mutable provenance: bla
owned by: james
owned by: myers
mutable description: bla
discontinued: false
dominant: false
experience: experience/None
group u list: _2443_
m user id: 30994
owner id: 26392
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: bla
star: false
creator: Thomas Dohmen, Milana Kremenovic, Yannick Kristiansen, Tim Maier
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: LVA-Tag
sequence annotation: GFP
sequence: https://synbiohub.org/public/igem/BBa_K1976005_sequence/1

LVA-Tag
type: SequenceAnnotation
persistent identity: annotation2518366
display id: annotation2518366
version: 1
top level: BBa_K1976005
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1976005/annotation2518366/range2518366/1

https://synbiohub.org/public/igem/BBa_K1976005/annotation2518366/range2518366/1
type: Range
persistent identity: range2518366
display id: range2518366
version: 1
top level: BBa_K1976005
direction: direction/forward
start: 715
end: 747
orientation: inline

GFP
type: SequenceAnnotation
persistent identity: annotation2518365
display id: annotation2518365
version: 1
top level: BBa_K1976005
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1976005/annotation2518365/range2518365/1

https://synbiohub.org/public/igem/BBa_K1976005/annotation2518365/range2518365/1
type: Range
persistent identity: range2518365
display id: range2518365
version: 1
top level: BBa_K1976005
direction: direction/forward
start: 1
end: 714
orientation: inline

Screening
type: ComponentDefinition
persistent identity: pSB1A10
display id: pSB1A10
version: 1
was generated by: iGEM to SBOL conversion
description: Screening Plasmid v1.0
created: 2006-05-25T11:00:00Z
modified: 2015-05-08T01:14:55Z
owned by: james
owned by: myers
discontinued: false
dominant: false
experience: experience/Works
group u list: _11_
m user id: 0
owner id: 2
owning group id: 84
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: true
creator: Josh Michener, Jason Kelly
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: RNAse E site
sequence annotation: Hairpin
sequence annotation: suffix
sequence annotation: B0015 (Term)
sequence annotation: spacer artifact from 13450
sequence annotation: I0500 (pBAD/araC)
sequence annotation: Scar from BB suffix removal by I13450
sequence annotation: B0034
sequence annotation: prefix
sequence annotation: Scar from BB prefix removal by I13450
sequence annotation: barcode
sequence annotation: psb1a3
sequence annotation: E0040 (EGFP)
sequence annotation: E1010 (mRFP1)
sequence: https://synbiohub.org/public/igem/pSB1A10_sequence/1

RNAse E site
type: SequenceAnnotation
persistent identity: annotation1871318
display id: annotation1871318
version: 1
top level: Screening
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB1A10/annotation1871318/range1871318/1

https://synbiohub.org/public/igem/pSB1A10/annotation1871318/range1871318/1
type: Range
persistent identity: range1871318
display id: range1871318
version: 1
top level: Screening
direction: direction/forward
start: 5203
end: 5223
orientation: inline

Hairpin
type: SequenceAnnotation
persistent identity: annotation1871307
display id: annotation1871307
version: 1
top level: Screening
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/pSB1A10/annotation1871307/range1871307/1

https://synbiohub.org/public/igem/pSB1A10/annotation1871307/range1871307/1
type: Range
persistent identity: range1871307
display id: range1871307
version: 1
top level: Screening
direction: direction/forward
start: 59
end: 93
orientation: inline

suffix
type: SequenceAnnotation
persistent identity: annotation1871283
display id: annotation1871283
version: 1
top level: Screening
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/pSB1A10/annotation1871283/range1871283/1

https://synbiohub.org/public/igem/pSB1A10/annotation1871283/range1871283/1
type: Range
persistent identity: range1871283
display id: range1871283
version: 1
top level: Screening
direction: direction/forward
start: 1
end: 21
orientation: inline

B0015 (Term)
type: SequenceAnnotation
persistent identity: annotation1871311
display id: annotation1871311
version: 1
top level: Screening
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB1A10/annotation1871311/range1871311/1

https://synbiohub.org/public/igem/pSB1A10/annotation1871311/range1871311/1
type: Range
persistent identity: range1871311
display id: range1871311
version: 1
top level: Screening
direction: direction/forward
start: 834
end: 962
orientation: inline

spacer artifact from 13450
type: SequenceAnnotation
persistent identity: annotation1871305
display id: annotation1871305
version: 1
top level: Screening
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB1A10/annotation1871305/range1871305/1

https://synbiohub.org/public/igem/pSB1A10/annotation1871305/range1871305/1
type: Range
persistent identity: range1871305
display id: range1871305
version: 1
top level: Screening
direction: direction/forward
start: 22
end: 37
orientation: inline

I0500 (pBAD/araC)
type: SequenceAnnotation
persistent identity: annotation1871315
display id: annotation1871315
version: 1
top level: Screening
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/pSB1A10/annotation1871315/range1871315/1

https://synbiohub.org/public/igem/pSB1A10/annotation1871315/range1871315/1
type: Range
persistent identity: range1871315
display id: range1871315
version: 1
top level: Screening
direction: direction/forward
start: 3239
end: 4448
orientation: inline

Scar from BB suffix removal by I13450
type: SequenceAnnotation
persistent identity: annotation1871312
display id: annotation1871312
version: 1
top level: Screening
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB1A10/annotation1871312/range1871312/1

https://synbiohub.org/public/igem/pSB1A10/annotation1871312/range1871312/1
type: Range
persistent identity: range1871312
display id: range1871312
version: 1
top level: Screening
direction: direction/forward
start: 963
end: 1044
orientation: inline

B0034
type: SequenceAnnotation
persistent identity: annotation1871308
display id: annotation1871308
version: 1
top level: Screening
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/pSB1A10/annotation1871308/range1871308/1

https://synbiohub.org/public/igem/pSB1A10/annotation1871308/range1871308/1
type: Range
persistent identity: range1871308
display id: range1871308
version: 1
top level: Screening
direction: direction/forward
start: 102
end: 113
orientation: inline

prefix
type: SequenceAnnotation
persistent identity: annotation1871320
display id: annotation1871320
version: 1
top level: Screening
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/pSB1A10/annotation1871320/range1871320/1

https://synbiohub.org/public/igem/pSB1A10/annotation1871320/range1871320/1
type: Range
persistent identity: range1871320
display id: range1871320
version: 1
top level: Screening
direction: direction/forward
start: 5242
end: 5263
orientation: inline

Scar from BB prefix removal by I13450
type: SequenceAnnotation
persistent identity: annotation1871314
display id: annotation1871314
version: 1
top level: Screening
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB1A10/annotation1871314/range1871314/1

https://synbiohub.org/public/igem/pSB1A10/annotation1871314/range1871314/1
type: Range
persistent identity: range1871314
display id: range1871314
version: 1
top level: Screening
direction: direction/forward
start: 3158
end: 3238
orientation: inline

RNAse E site
type: SequenceAnnotation
persistent identity: annotation1871306
display id: annotation1871306
version: 1
top level: Screening
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB1A10/annotation1871306/range1871306/1

https://synbiohub.org/public/igem/pSB1A10/annotation1871306/range1871306/1
type: Range
persistent identity: range1871306
display id: range1871306
version: 1
top level: Screening
direction: direction/forward
start: 38
end: 58
orientation: inline

B0034
type: SequenceAnnotation
persistent identity: annotation1871316
display id: annotation1871316
version: 1
top level: Screening
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/pSB1A10/annotation1871316/range1871316/1

https://synbiohub.org/public/igem/pSB1A10/annotation1871316/range1871316/1
type: Range
persistent identity: range1871316
display id: range1871316
version: 1
top level: Screening
direction: direction/forward
start: 4457
end: 4468
orientation: inline

barcode
type: SequenceAnnotation
persistent identity: annotation1871310
display id: annotation1871310
version: 1
top level: Screening
role: SO:0000807
role: feature/barcode
location: https://synbiohub.org/public/igem/pSB1A10/annotation1871310/range1871310/1

https://synbiohub.org/public/igem/pSB1A10/annotation1871310/range1871310/1
type: Range
persistent identity: range1871310
display id: range1871310
version: 1
top level: Screening
direction: direction/forward
start: 801
end: 825
orientation: inline

psb1a3
type: SequenceAnnotation
persistent identity: annotation1871313
display id: annotation1871313
version: 1
top level: Screening
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB1A10/annotation1871313/range1871313/1

https://synbiohub.org/public/igem/pSB1A10/annotation1871313/range1871313/1
type: Range
persistent identity: range1871313
display id: range1871313
version: 1
top level: Screening
direction: direction/forward
start: 1045
end: 3157
orientation: inline

spacer artifact from 13450
type: SequenceAnnotation
persistent identity: annotation1871319
display id: annotation1871319
version: 1
top level: Screening
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB1A10/annotation1871319/range1871319/1

https://synbiohub.org/public/igem/pSB1A10/annotation1871319/range1871319/1
type: Range
persistent identity: range1871319
display id: range1871319
version: 1
top level: Screening
direction: direction/forward
start: 5224
end: 5241
orientation: inline

E0040 (EGFP)
type: SequenceAnnotation
persistent identity: annotation1871317
display id: annotation1871317
version: 1
top level: Screening
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/pSB1A10/annotation1871317/range1871317/1

https://synbiohub.org/public/igem/pSB1A10/annotation1871317/range1871317/1
type: Range
persistent identity: range1871317
display id: range1871317
version: 1
top level: Screening
direction: direction/forward
start: 4475
end: 5194
orientation: inline

E1010 (mRFP1)
type: SequenceAnnotation
persistent identity: annotation1871309
display id: annotation1871309
version: 1
top level: Screening
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/pSB1A10/annotation1871309/range1871309/1

https://synbiohub.org/public/igem/pSB1A10/annotation1871309/range1871309/1
type: Range
persistent identity: range1871309
display id: range1871309
version: 1
top level: Screening
direction: direction/forward
start: 120
end: 800
orientation: inline

BBa_K1937005
type: ComponentDefinition
persistent identity: BBa K1937005
display id: BBa_K1937005
version: 1
was generated by: iGEM to SBOL conversion
description: pNagP-RFP
created: 2016-10-12T11:00:00Z
modified: 2016-10-17T01:34:08Z
mutable provenance: In bacillus subtilis, this promoter has been reported to induce expression in presence of N-acetylglucosamine 6-phosphate.
owned by: james
owned by: myers
mutable description: pNagP is the promoter of the NagP gene, which expresses a transporter of N-acetylglucosamine-6-phosphate in Bacillus subtilis. In bacillus subtilis, this promoter has been reported to induce expression in presence of N-acetylglucosamine 6-phosphate. This NAG molecule is the major component of chitin, a characteristic molecule of fungi. This BioBrick is a part developed by the Toulouse 2016 iGEM team (http://2016.igem.org/Team:Toulouse_France)
discontinued: false
dominant: false
experience: experience/Works
group u list: _2404_
m user id: 21497
owner id: 30403
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: The BBa_K1937005 part was designed to demonstrate the induction of pNagP by NAG. It belongs to the antifungal module in the Paleolitis project of iGEM Toulouse 2016 (http://2016.igem.org/Team:Toulouse_France). The part includes the pNagP promoter and its RBS (position 840,455 to 840,655 of the Bacillus genome). The promoter controls the expression of the RFP fluorescent reporter gene. A NheI restriction site has been placed before the RFP ORF to facilitate promoter swapping.
star: false
creator: Oumnia KARIM
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: Promoter NagP+RBS
sequence annotation: RFP
sequence annotation: Terminator
sequence: https://synbiohub.org/public/igem/BBa_K1937005_sequence/1

Promoter NagP+RBS
type: SequenceAnnotation
persistent identity: annotation2499134
display id: annotation2499134
version: 1
top level: BBa_K1937005
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1937005/annotation2499134/range2499134/1

https://synbiohub.org/public/igem/BBa_K1937005/annotation2499134/range2499134/1
type: Range
persistent identity: range2499134
display id: range2499134
version: 1
top level: BBa_K1937005
direction: direction/forward
start: 1
end: 200
orientation: inline

RFP
type: SequenceAnnotation
persistent identity: annotation2499156
display id: annotation2499156
version: 1
top level: BBa_K1937005
role: feature/protein
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1937005/annotation2499156/range2499156/1

https://synbiohub.org/public/igem/BBa_K1937005/annotation2499156/range2499156/1
type: Range
persistent identity: range2499156
display id: range2499156
version: 1
top level: BBa_K1937005
direction: direction/forward
start: 207
end: 912
orientation: inline

Terminator
type: SequenceAnnotation
persistent identity: annotation2499158
display id: annotation2499158
version: 1
top level: BBa_K1937005
role: feature/stop
role: SO:0000319
location: https://synbiohub.org/public/igem/BBa_K1937005/annotation2499158/range2499158/1

https://synbiohub.org/public/igem/BBa_K1937005/annotation2499158/range2499158/1
type: Range
persistent identity: range2499158
display id: range2499158
version: 1
top level: BBa_K1937005
direction: direction/forward
start: 913
end: 992
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: stem_loop
sequence annotation: BBa_B0010
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

BBa_J23101
type: ComponentDefinition
persistent identity: BBa J23101
display id: BBa_J23101
version: 1
was generated by: iGEM to SBOL conversion
description: constitutive promoter family member
created: 2006-08-03T11:00:00Z
modified: 2015-08-31T04:08:40Z
mutable provenance: later
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: later
discontinued: false
dominant: true
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: true
mutable notes: N/A
star: true
creator: John Anderson
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J23101_sequence/1

BBa_K319043
type: ComponentDefinition
persistent identity: BBa K319043
display id: BBa_K319043
version: 1
was generated by: iGEM to SBOL conversion
description: ADE4 targeting vector
created: 2010-10-25T11:00:00Z
modified: 2015-05-08T01:11:59Z
mutable provenance: n
owned by: james
owned by: myers
mutable description: This is a targeting vector for homologous recombination into the ADE4 locus of S. cerevisiae. This vector can be used in cloning free cloning (ref. 2010 uOttawa iGEM wiki). Yeast strains with an ADE1 or ADE2 deletion or mutation have red colonies; by integrating the constructs using our protocol the ADE4 gene is knocked out and the colonies express a white phenotype.
discontinued: false
dominant: false
experience: experience/Works
group u list: _437_
m user id: 0
owner id: 7363
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: N/A
star: false
creator: Afnan Azizi
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: PstI site
sequence annotation: Reverse landing pad
sequence annotation: G5C5G5 filler site
sequence annotation: Forward landing pad
sequence annotation: Cm resistance
sequence annotation: ADE4 terminator
sequence annotation: ATM1 terminator
sequence annotation: EcoRI
sequence annotation: EcoRI/MfeI mixed site
sequence annotation: PstI/NsiI mixed site
sequence: https://synbiohub.org/public/igem/BBa_K319043_sequence/1

PstI site
type: SequenceAnnotation
persistent identity: annotation2109295
display id: annotation2109295
version: 1
top level: BBa_K319043
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K319043/annotation2109295/range2109295/1

https://synbiohub.org/public/igem/BBa_K319043/annotation2109295/range2109295/1
type: Range
persistent identity: range2109295
display id: range2109295
version: 1
top level: BBa_K319043
direction: direction/forward
start: 248
end: 253
orientation: inline

Reverse landing pad
type: SequenceAnnotation
persistent identity: annotation2109297
display id: annotation2109297
version: 1
top level: BBa_K319043
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K319043/annotation2109297/range2109297/1

https://synbiohub.org/public/igem/BBa_K319043/annotation2109297/range2109297/1
type: Range
persistent identity: range2109297
display id: range2109297
version: 1
top level: BBa_K319043
direction: direction/reverse
start: 254
end: 273
orientation: inline

G5C5G5 filler site
type: SequenceAnnotation
persistent identity: annotation2109294
display id: annotation2109294
version: 1
top level: BBa_K319043
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K319043/annotation2109294/range2109294/1

https://synbiohub.org/public/igem/BBa_K319043/annotation2109294/range2109294/1
type: Range
persistent identity: range2109294
display id: range2109294
version: 1
top level: BBa_K319043
direction: direction/forward
start: 233
end: 247
orientation: inline

Forward landing pad
type: SequenceAnnotation
persistent identity: annotation2109296
display id: annotation2109296
version: 1
top level: BBa_K319043
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K319043/annotation2109296/range2109296/1

https://synbiohub.org/public/igem/BBa_K319043/annotation2109296/range2109296/1
type: Range
persistent identity: range2109296
display id: range2109296
version: 1
top level: BBa_K319043
direction: direction/forward
start: 207
end: 226
orientation: inline

Cm resistance
type: SequenceAnnotation
persistent identity: annotation2109321
display id: annotation2109321
version: 1
top level: BBa_K319043
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K319043/annotation2109321/range2109321/1

https://synbiohub.org/public/igem/BBa_K319043/annotation2109321/range2109321/1
type: Range
persistent identity: range2109321
display id: range2109321
version: 1
top level: BBa_K319043
direction: direction/reverse
start: 1620
end: 2279
orientation: inline

ADE4 terminator
type: SequenceAnnotation
persistent identity: annotation2109298
display id: annotation2109298
version: 1
top level: BBa_K319043
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K319043/annotation2109298/range2109298/1

https://synbiohub.org/public/igem/BBa_K319043/annotation2109298/range2109298/1
type: Range
persistent identity: range2109298
display id: range2109298
version: 1
top level: BBa_K319043
direction: direction/forward
start: 274
end: 473
orientation: inline

ATM1 terminator
type: SequenceAnnotation
persistent identity: annotation2109292
display id: annotation2109292
version: 1
top level: BBa_K319043
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K319043/annotation2109292/range2109292/1

https://synbiohub.org/public/igem/BBa_K319043/annotation2109292/range2109292/1
type: Range
persistent identity: range2109292
display id: range2109292
version: 1
top level: BBa_K319043
direction: direction/forward
start: 7
end: 206
orientation: inline

EcoRI
type: SequenceAnnotation
persistent identity: annotation2109293
display id: annotation2109293
version: 1
top level: BBa_K319043
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K319043/annotation2109293/range2109293/1

https://synbiohub.org/public/igem/BBa_K319043/annotation2109293/range2109293/1
type: Range
persistent identity: range2109293
display id: range2109293
version: 1
top level: BBa_K319043
direction: direction/forward
start: 227
end: 232
orientation: inline

EcoRI/MfeI mixed site
type: SequenceAnnotation
persistent identity: annotation2109290
display id: annotation2109290
version: 1
top level: BBa_K319043
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K319043/annotation2109290/range2109290/1

https://synbiohub.org/public/igem/BBa_K319043/annotation2109290/range2109290/1
type: Range
persistent identity: range2109290
display id: range2109290
version: 1
top level: BBa_K319043
direction: direction/forward
start: 1
end: 6
orientation: inline

PstI/NsiI mixed site
type: SequenceAnnotation
persistent identity: annotation2109301
display id: annotation2109301
version: 1
top level: BBa_K319043
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K319043/annotation2109301/range2109301/1

https://synbiohub.org/public/igem/BBa_K319043/annotation2109301/range2109301/1
type: Range
persistent identity: range2109301
display id: range2109301
version: 1
top level: BBa_K319043
direction: direction/forward
start: 474
end: 479
orientation: inline

pSB2K0
type: ComponentDefinition
persistent identity: pSB2K0
display id: pSB2K0
version: 1
was generated by: iGEM to SBOL conversion
description: pSB2K0 (pSCANS-BNL; Replaced by pSB2K3)
created: 2005-06-12T11:00:00Z
modified: 2015-05-08T01:14:55Z
owned by: james
owned by: myers
mutable description: Warning, this plasmid does not have a proper BioBrick cloning site.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _41_1_
m user id: 0
owner id: 25
owning group id: 1
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: "A new plasmid vector, pSCANS, has been constructed which allows rapid generation of an ordered set of nested deletions from either strand of a cloned DNA fragment. pSCANS is based on the low-copy F replicon. The size of the vector DNA has been reduced to the 4.4-kbp range by removing the 2.5-kbp sop (stability of plasmid genes) region. The resulting plasmid has the low copy number typical of F plasmids and it remains stable enough to be easily maintained by growth in the presence of kanamycin, the selective antibiotic. DNA in amounts convenient for sequencing is readily obtained by amplification from an IPTG-inducible P1 lytic replicon. The vector's multiple cloning region (MCR), which has several unique sites for both shotgun and directional cloning, is flanked on one side by recognition sequences for the extremely rare cutting intron encoded nucleases I-CeuI and I-SceI, and on the other side by a recognition sequence for another intron encoded enzyme, PI-PspI and a nicking site for the phage f1 protein, gpII, that initiates f1 rolling circle DNA replication. Cleavage with the intron encoded enzymes leaves four-base 3' overhangs that are resistant to digestion with E. coli ExoIII. Between these sites and the MCR are recognition sites for several rare 8-base cutters that leave ExoIII sensitive termini. Double cutting with one intron encoded enzyme and an adjacent rare cutting restriction endonuclease allows for unidirectional 3' to 5' digestion across the insert with ExoIII. Alternatively, plasmid linearized with I-SceI can be blunt ended to produce an ExoIII sensitive end an ent generates a good distribution of deletion clones following electroporation. Deletion clones are sized and sequenced using vector specific forward and reverse primers. "
star: false
creator: Brookhaven National Lab
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: kanR
sequence: https://synbiohub.org/public/igem/pSB2K0_sequence/1

kanR
type: SequenceAnnotation
persistent identity: annotation1782703
display id: annotation1782703
version: 1
top level: pSB2K0
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/pSB2K0/annotation1782703/range1782703/1

https://synbiohub.org/public/igem/pSB2K0/annotation1782703/range1782703/1
type: Range
persistent identity: range1782703
display id: range1782703
version: 1
top level: pSB2K0
direction: direction/forward
start: 1
end: 816
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

BBa_K1976000
type: ComponentDefinition
persistent identity: BBa K1976000
display id: BBa_K1976000
version: 1
was generated by: iGEM to SBOL conversion
description: Integration plasmid
created: 2016-10-12T11:00:00Z
modified: 2016-10-18T01:58:01Z
mutable provenance: BBa_I11023
owned by: james
owned by: myers
mutable description: Integration plasmid
discontinued: false
dominant: false
experience: experience/None
group u list: _2443_
m user id: 30994
owner id: 26981
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: .
star: false
creator: Thomas Dohmen, Milana Kremenovic, Yannick Kristiansen, Tim Maier
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1976006
component: BBa_K1976004
component: BBa_J23101
sequence annotation: BBa_K1976006
sequence annotation: BBa_K1976004
sequence annotation: BBa_J23101
sequence: https://synbiohub.org/public/igem/BBa_K1976000_sequence/1

BBa_K1976006
type: Component
persistent identity: component2518416
display id: component2518416
version: 1
top level: BBa_K1976000
access: public
definition: BBa_K1976006

BBa_K1976004
type: Component
persistent identity: component2518402
display id: component2518402
version: 1
top level: BBa_K1976000
access: public
definition: BBa_K1976004

BBa_J23101
type: Component
persistent identity: component2518403
display id: component2518403
version: 1
top level: BBa_K1976000
access: public
definition: BBa_J23101

BBa_K1976006
type: SequenceAnnotation
persistent identity: annotation2518416
display id: annotation2518416
version: 1
top level: BBa_K1976000
location: https://synbiohub.org/public/igem/BBa_K1976000/annotation2518416/range2518416/1
component: BBa_K1976006

https://synbiohub.org/public/igem/BBa_K1976000/annotation2518416/range2518416/1
type: Range
persistent identity: range2518416
display id: range2518416
version: 1
top level: BBa_K1976000
direction: direction/reverse
start: 352
end: 1260
orientation: inline

BBa_K1976004
type: SequenceAnnotation
persistent identity: annotation2518402
display id: annotation2518402
version: 1
top level: BBa_K1976000
location: https://synbiohub.org/public/igem/BBa_K1976000/annotation2518402/range2518402/1
component: BBa_K1976004

https://synbiohub.org/public/igem/BBa_K1976000/annotation2518402/range2518402/1
type: Range
persistent identity: range2518402
display id: range2518402
version: 1
top level: BBa_K1976000
direction: direction/reverse
start: 1
end: 300
orientation: inline

BBa_J23101
type: SequenceAnnotation
persistent identity: annotation2518403
display id: annotation2518403
version: 1
top level: BBa_K1976000
location: https://synbiohub.org/public/igem/BBa_K1976000/annotation2518403/range2518403/1
component: BBa_J23101

https://synbiohub.org/public/igem/BBa_K1976000/annotation2518403/range2518403/1
type: Range
persistent identity: range2518403
display id: range2518403
version: 1
top level: BBa_K1976000
direction: direction/reverse
start: 309
end: 343
orientation: inline

BBa_I11006
type: ComponentDefinition
persistent identity: BBa I11006
display id: BBa_I11006
version: 1
was generated by: iGEM to SBOL conversion
description: pZE1PLlacO-1 plasmid
created: 2004-08-17T11:00:00Z
modified: 2015-08-31T04:07:30Z
owned by: james
owned by: myers
mutable description: This plasmid has the ColE1 origin of replication, amp resistance marker, lac0-1 promoter, and multiple cloning sites.
discontinued: false
dominant: false
experience: experience/None
group u list: _2_
m user id: 0
owner id: 102
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Lutz and Bujard
type: DnaRegion
role: SO:0000155
role: partType/Plasmid
sequence: https://synbiohub.org/public/igem/BBa_I11006_sequence/1

BBa_K1976006
type: ComponentDefinition
persistent identity: BBa K1976006
display id: BBa_K1976006
version: 1
was generated by: iGEM to SBOL conversion
description: GFP generator with LVA-tag
created: 2016-10-13T11:00:00Z
modified: 2016-10-18T01:57:33Z
mutable provenance: bla
owned by: james
owned by: myers
mutable description: bla
discontinued: false
dominant: false
experience: experience/None
group u list: _2443_
m user id: 30994
owner id: 26392
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: bla
star: false
creator: Thomas Dohmen, Milana Kremenovic, Yannick Kristiansen, Tim Maier
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0032
component: BBa_B0012
component: BBa_K1976005
component: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_B0010
sequence annotation: BBa_K1976005
sequence annotation: BBa_B0032
sequence: https://synbiohub.org/public/igem/BBa_K1976006_sequence/1

BBa_B0032
type: Component
persistent identity: component2518387
display id: component2518387
version: 1
top level: BBa_K1976006
access: public
definition: BBa_B0032

BBa_B0012
type: Component
persistent identity: component2518394
display id: component2518394
version: 1
top level: BBa_K1976006
access: public
definition: BBa_B0012

BBa_K1976005
type: Component
persistent identity: component2518391
display id: component2518391
version: 1
top level: BBa_K1976006
access: public
definition: BBa_K1976005

BBa_B0010
type: Component
persistent identity: component2518392
display id: component2518392
version: 1
top level: BBa_K1976006
access: public
definition: BBa_B0010

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2518394
display id: annotation2518394
version: 1
top level: BBa_K1976006
location: https://synbiohub.org/public/igem/BBa_K1976006/annotation2518394/range2518394/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K1976006/annotation2518394/range2518394/1
type: Range
persistent identity: range2518394
display id: range2518394
version: 1
top level: BBa_K1976006
direction: direction/forward
start: 869
end: 909
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2518392
display id: annotation2518392
version: 1
top level: BBa_K1976006
location: https://synbiohub.org/public/igem/BBa_K1976006/annotation2518392/range2518392/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K1976006/annotation2518392/range2518392/1
type: Range
persistent identity: range2518392
display id: range2518392
version: 1
top level: BBa_K1976006
direction: direction/forward
start: 781
end: 860
orientation: inline

BBa_K1976005
type: SequenceAnnotation
persistent identity: annotation2518391
display id: annotation2518391
version: 1
top level: BBa_K1976006
location: https://synbiohub.org/public/igem/BBa_K1976006/annotation2518391/range2518391/1
component: BBa_K1976005

https://synbiohub.org/public/igem/BBa_K1976006/annotation2518391/range2518391/1
type: Range
persistent identity: range2518391
display id: range2518391
version: 1
top level: BBa_K1976006
direction: direction/reverse
start: 20
end: 772
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation2518387
display id: annotation2518387
version: 1
top level: BBa_K1976006
location: https://synbiohub.org/public/igem/BBa_K1976006/annotation2518387/range2518387/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_K1976006/annotation2518387/range2518387/1
type: Range
persistent identity: range2518387
display id: range2518387
version: 1
top level: BBa_K1976006
direction: direction/forward
start: 1
end: 13
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: polya
sequence annotation: T7 TE
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

pSB1A1
type: ComponentDefinition
persistent identity: pSB1A1
display id: pSB1A1
version: 1
was generated by: iGEM to SBOL conversion
description: pUC19 with a BioBrick cloning site (Replaced by pSB1A3)
created: 2006-10-23T11:00:00Z
modified: 2015-05-08T01:14:55Z
mutable provenance: pUC19 is an extremely common cloning vector. This particular version is entered in the registry because several parts synthesized by Codon Devices (BBa_P1002, BBa_P1003, BBa_P1004, BBa_P1005, BBa_I50040) were returned in this vector. Sequence information can be found at http://www.fermentas.com/techinfo/nucleicacids/sequences/puc19.txt
owned by: james
owned by: myers
mutable description: This plasmid is pUC19 with a BioBricks cloning site (EcoRI-NotI-XbaI-SpeI-NotI-PstI) located between the EcoRI and BamHI sites in the multiple cloning site of pUC19.
discontinued: false
dominant: false
experience: experience/None
group u list: _41_
m user id: 0
owner id: 126
owning group id: 70
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: It is not entirely clear whether there are two contigous copies of EcoRI restriction site or one copy of the EcoRI in this vector. Sequence information did not include this section of the vector. In this part, only a single EcoRI restriction site is shown.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: RNAII transcript
sequence annotation: origin of replication
sequence annotation: pBR322
sequence annotation: M13 Forward priming site
sequence annotation: RNAI transcript promoter -35 site
sequence annotation: P(BLA) -35 site
sequence annotation: BioBricks prefix
sequence annotation: RNAII transcript promoter -35 site
sequence annotation: ORI
sequence annotation: P(LAC) transcription start site
sequence annotation: beta-lactamase (bla; amp-r)
sequence annotation: bla RNA transcription start
sequence annotation: RNAI transcript
sequence annotation: beta-lactamase signal peptide
sequence annotation: CAP protein binding site
sequence annotation: P(LAC) -35 site
sequence annotation: M13Reverse priming site
sequence annotation: Lac operon
sequence annotation: P(LAC) -10 site
sequence annotation: P(BLA) -10 site
sequence annotation: BioBricks suffix
sequence annotation: RNAII transcript promoter -10 site
sequence: https://synbiohub.org/public/igem/pSB1A1_sequence/1

RNAII transcript
type: SequenceAnnotation
persistent identity: annotation1904905
display id: annotation1904905
version: 1
top level: pSB1A1
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB1A1/annotation1904905/range1904905/1

https://synbiohub.org/public/igem/pSB1A1/annotation1904905/range1904905/1
type: Range
persistent identity: range1904905
display id: range1904905
version: 1
top level: pSB1A1
direction: direction/reverse
start: 472
end: 1024
orientation: inline

origin of replication
type: SequenceAnnotation
persistent identity: annotation1904906
display id: annotation1904906
version: 1
top level: pSB1A1
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB1A1/annotation1904906/range1904906/1

https://synbiohub.org/public/igem/pSB1A1/annotation1904906/range1904906/1
type: Range
persistent identity: range1904906
display id: range1904906
version: 1
top level: pSB1A1
direction: direction/reverse
start: 472
end: 1060
orientation: inline

pBR322
type: SequenceAnnotation
persistent identity: annotation1904912
display id: annotation1904912
version: 1
top level: pSB1A1
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB1A1/annotation1904912/range1904912/1

https://synbiohub.org/public/igem/pSB1A1/annotation1904912/range1904912/1
type: Range
persistent identity: range1904912
display id: range1904912
version: 1
top level: pSB1A1
direction: direction/forward
start: 290
end: 2291
orientation: inline

M13 Forward priming site
type: SequenceAnnotation
persistent identity: annotation1904919
display id: annotation1904919
version: 1
top level: pSB1A1
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/pSB1A1/annotation1904919/range1904919/1

https://synbiohub.org/public/igem/pSB1A1/annotation1904919/range1904919/1
type: Range
persistent identity: range1904919
display id: range1904919
version: 1
top level: pSB1A1
direction: direction/forward
start: 2670
end: 2685
orientation: inline

RNAI transcript promoter -35 site
type: SequenceAnnotation
persistent identity: annotation1904909
display id: annotation1904909
version: 1
top level: pSB1A1
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/pSB1A1/annotation1904909/range1904909/1

https://synbiohub.org/public/igem/pSB1A1/annotation1904909/range1904909/1
type: Range
persistent identity: range1904909
display id: range1904909
version: 1
top level: pSB1A1
direction: direction/forward
start: 900
end: 906
orientation: inline

P(BLA) -35 site
type: SequenceAnnotation
persistent identity: annotation1904917
display id: annotation1904917
version: 1
top level: pSB1A1
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/pSB1A1/annotation1904917/range1904917/1

https://synbiohub.org/public/igem/pSB1A1/annotation1904917/range1904917/1
type: Range
persistent identity: range1904917
display id: range1904917
version: 1
top level: pSB1A1
direction: direction/reverse
start: 2156
end: 2161
orientation: inline

BioBricks prefix
type: SequenceAnnotation
persistent identity: annotation1904921
display id: annotation1904921
version: 1
top level: pSB1A1
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB1A1/annotation1904921/range1904921/1

https://synbiohub.org/public/igem/pSB1A1/annotation1904921/range1904921/1
type: Range
persistent identity: range1904921
display id: range1904921
version: 1
top level: pSB1A1
direction: direction/forward
start: 2687
end: 2708
orientation: inline

RNAII transcript promoter -35 site
type: SequenceAnnotation
persistent identity: annotation1904911
display id: annotation1904911
version: 1
top level: pSB1A1
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/pSB1A1/annotation1904911/range1904911/1

https://synbiohub.org/public/igem/pSB1A1/annotation1904911/range1904911/1
type: Range
persistent identity: range1904911
display id: range1904911
version: 1
top level: pSB1A1
direction: direction/reverse
start: 1055
end: 1060
orientation: inline

ORI
type: SequenceAnnotation
persistent identity: annotation1904904
display id: annotation1904904
version: 1
top level: pSB1A1
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB1A1/annotation1904904/range1904904/1

https://synbiohub.org/public/igem/pSB1A1/annotation1904904/range1904904/1
type: Range
persistent identity: range1904904
display id: range1904904
version: 1
top level: pSB1A1
direction: direction/forward
start: 472
end: 472
orientation: inline

P(LAC) transcription start site
type: SequenceAnnotation
persistent identity: annotation1904899
display id: annotation1904899
version: 1
top level: pSB1A1
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB1A1/annotation1904899/range1904899/1

https://synbiohub.org/public/igem/pSB1A1/annotation1904899/range1904899/1
type: Range
persistent identity: range1904899
display id: range1904899
version: 1
top level: pSB1A1
direction: direction/reverse
start: 112
end: 112
orientation: inline

beta-lactamase (bla; amp-r)
type: SequenceAnnotation
persistent identity: annotation1904913
display id: annotation1904913
version: 1
top level: pSB1A1
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/pSB1A1/annotation1904913/range1904913/1

https://synbiohub.org/public/igem/pSB1A1/annotation1904913/range1904913/1
type: Range
persistent identity: range1904913
display id: range1904913
version: 1
top level: pSB1A1
direction: direction/reverse
start: 1231
end: 2091
orientation: inline

bla RNA transcription start
type: SequenceAnnotation
persistent identity: annotation1904915
display id: annotation1904915
version: 1
top level: pSB1A1
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB1A1/annotation1904915/range1904915/1

https://synbiohub.org/public/igem/pSB1A1/annotation1904915/range1904915/1
type: Range
persistent identity: range1904915
display id: range1904915
version: 1
top level: pSB1A1
direction: direction/reverse
start: 2126
end: 2126
orientation: inline

RNAI transcript
type: SequenceAnnotation
persistent identity: annotation1904907
display id: annotation1904907
version: 1
top level: pSB1A1
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB1A1/annotation1904907/range1904907/1

https://synbiohub.org/public/igem/pSB1A1/annotation1904907/range1904907/1
type: Range
persistent identity: range1904907
display id: range1904907
version: 1
top level: pSB1A1
direction: direction/forward
start: 914
end: 1021
orientation: inline

pBR322
type: SequenceAnnotation
persistent identity: annotation1904918
display id: annotation1904918
version: 1
top level: pSB1A1
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB1A1/annotation1904918/range1904918/1

https://synbiohub.org/public/igem/pSB1A1/annotation1904918/range1904918/1
type: Range
persistent identity: range1904918
display id: range1904918
version: 1
top level: pSB1A1
direction: direction/forward
start: 2292
end: 2428
orientation: inline

beta-lactamase signal peptide
type: SequenceAnnotation
persistent identity: annotation1904914
display id: annotation1904914
version: 1
top level: pSB1A1
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/pSB1A1/annotation1904914/range1904914/1

https://synbiohub.org/public/igem/pSB1A1/annotation1904914/range1904914/1
type: Range
persistent identity: range1904914
display id: range1904914
version: 1
top level: pSB1A1
direction: direction/reverse
start: 2023
end: 2091
orientation: inline

CAP protein binding site
type: SequenceAnnotation
persistent identity: annotation1904902
display id: annotation1904902
version: 1
top level: pSB1A1
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/pSB1A1/annotation1904902/range1904902/1

https://synbiohub.org/public/igem/pSB1A1/annotation1904902/range1904902/1
type: Range
persistent identity: range1904902
display id: range1904902
version: 1
top level: pSB1A1
direction: direction/reverse
start: 168
end: 180
orientation: inline

P(LAC) -35 site
type: SequenceAnnotation
persistent identity: annotation1904901
display id: annotation1904901
version: 1
top level: pSB1A1
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/pSB1A1/annotation1904901/range1904901/1

https://synbiohub.org/public/igem/pSB1A1/annotation1904901/range1904901/1
type: Range
persistent identity: range1904901
display id: range1904901
version: 1
top level: pSB1A1
direction: direction/reverse
start: 143
end: 148
orientation: inline

M13Reverse priming site
type: SequenceAnnotation
persistent identity: annotation1904898
display id: annotation1904898
version: 1
top level: pSB1A1
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/pSB1A1/annotation1904898/range1904898/1

https://synbiohub.org/public/igem/pSB1A1/annotation1904898/range1904898/1
type: Range
persistent identity: range1904898
display id: range1904898
version: 1
top level: pSB1A1
direction: direction/reverse
start: 70
end: 86
orientation: inline

Lac operon
type: SequenceAnnotation
persistent identity: annotation1904903
display id: annotation1904903
version: 1
top level: pSB1A1
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB1A1/annotation1904903/range1904903/1

https://synbiohub.org/public/igem/pSB1A1/annotation1904903/range1904903/1
type: Range
persistent identity: range1904903
display id: range1904903
version: 1
top level: pSB1A1
direction: direction/reverse
start: 60
end: 289
orientation: inline

P(LAC) -10 site
type: SequenceAnnotation
persistent identity: annotation1904900
display id: annotation1904900
version: 1
top level: pSB1A1
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/pSB1A1/annotation1904900/range1904900/1

https://synbiohub.org/public/igem/pSB1A1/annotation1904900/range1904900/1
type: Range
persistent identity: range1904900
display id: range1904900
version: 1
top level: pSB1A1
direction: direction/reverse
start: 119
end: 124
orientation: inline

P(BLA) -10 site
type: SequenceAnnotation
persistent identity: annotation1904916
display id: annotation1904916
version: 1
top level: pSB1A1
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/pSB1A1/annotation1904916/range1904916/1

https://synbiohub.org/public/igem/pSB1A1/annotation1904916/range1904916/1
type: Range
persistent identity: range1904916
display id: range1904916
version: 1
top level: pSB1A1
direction: direction/reverse
start: 2135
end: 2140
orientation: inline

BioBricks suffix
type: SequenceAnnotation
persistent identity: annotation1904920
display id: annotation1904920
version: 1
top level: pSB1A1
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB1A1/annotation1904920/range1904920/1

https://synbiohub.org/public/igem/pSB1A1/annotation1904920/range1904920/1
type: Range
persistent identity: range1904920
display id: range1904920
version: 1
top level: pSB1A1
direction: direction/forward
start: 1
end: 21
orientation: inline

RNAII transcript promoter -10 site
type: SequenceAnnotation
persistent identity: annotation1904910
display id: annotation1904910
version: 1
top level: pSB1A1
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/pSB1A1/annotation1904910/range1904910/1

https://synbiohub.org/public/igem/pSB1A1/annotation1904910/range1904910/1
type: Range
persistent identity: range1904910
display id: range1904910
version: 1
top level: pSB1A1
direction: direction/reverse
start: 1034
end: 1039
orientation: inline

RNAI transcript promoter -35 site
type: SequenceAnnotation
persistent identity: annotation1904908
display id: annotation1904908
version: 1
top level: pSB1A1
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/pSB1A1/annotation1904908/range1904908/1

https://synbiohub.org/public/igem/pSB1A1/annotation1904908/range1904908/1
type: Range
persistent identity: range1904908
display id: range1904908
version: 1
top level: pSB1A1
direction: direction/forward
start: 878
end: 883
orientation: inline

pSB1AT3-1
type: ComponentDefinition
persistent identity: pSB1AT3 1
display id: pSB1AT3_1
version: 1
was generated by: iGEM to SBOL conversion
description: pSB1AT3 with ccdB insert
created: 2005-06-06T11:00:00Z
modified: 2015-05-08T01:14:55Z
owned by: james
owned by: myers
mutable description: Target plasmid for three antibiotic assembly. Tetracycline resistant. Contains an insert with a constitutive promoter and the ccdB coding region. The ccdB gene expression is lethal in normal cloning strains. The plasmid must be replicated in the special ccdB resistant strain DB3.1.
discontinued: true
dominant: false
experience: experience/Works
group u list: _42_
m user id: 0
owner id: 6
owning group id: 42
sample status: Discontinued
status: status/Deleted
bookmark: false
star: false
creator: Tom Knight
type: DnaRegion
role: SO:0000155
role: partType/Plasmid
sequence annotation: Tet-r
sequence annotation: terminator
sequence annotation: BB-Post
sequence annotation: rep (pMB1) (rev)
sequence annotation: ORI
sequence annotation: ccdA- (rev)
sequence annotation: VF2
sequence annotation: ccdB (rev)
sequence annotation: VR
sequence annotation: BB-Pre
sequence annotation: APr (rev)
sequence: https://synbiohub.org/public/igem/pSB1AT3_1_sequence/1

Tet-r
type: SequenceAnnotation
persistent identity: annotation1569250
display id: annotation1569250
version: 1
top level: pSB1AT3-1
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/pSB1AT3_1/annotation1569250/range1569250/1

https://synbiohub.org/public/igem/pSB1AT3_1/annotation1569250/range1569250/1
type: Range
persistent identity: range1569250
display id: range1569250
version: 1
top level: pSB1AT3-1
direction: direction/forward
start: 1942
end: 3132
orientation: inline

terminator
type: SequenceAnnotation
persistent identity: annotation1569334
display id: annotation1569334
version: 1
top level: pSB1AT3-1
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/pSB1AT3_1/annotation1569334/range1569334/1

https://synbiohub.org/public/igem/pSB1AT3_1/annotation1569334/range1569334/1
type: Range
persistent identity: range1569334
display id: range1569334
version: 1
top level: pSB1AT3-1
direction: direction/forward
start: 845
end: 879
orientation: inline

BB-Post
type: SequenceAnnotation
persistent identity: annotation1569273
display id: annotation1569273
version: 1
top level: pSB1AT3-1
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/pSB1AT3_1/annotation1569273/range1569273/1

https://synbiohub.org/public/igem/pSB1AT3_1/annotation1569273/range1569273/1
type: Range
persistent identity: range1569273
display id: range1569273
version: 1
top level: pSB1AT3-1
direction: direction/forward
start: 818
end: 836
orientation: inline

rep (pMB1) (rev)
type: SequenceAnnotation
persistent identity: annotation1569289
display id: annotation1569289
version: 1
top level: pSB1AT3-1
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB1AT3_1/annotation1569289/range1569289/1

https://synbiohub.org/public/igem/pSB1AT3_1/annotation1569289/range1569289/1
type: Range
persistent identity: range1569289
display id: range1569289
version: 1
top level: pSB1AT3-1
direction: direction/forward
start: 1076
end: 1690
orientation: inline

ORI
type: SequenceAnnotation
persistent identity: annotation1569319
display id: annotation1569319
version: 1
top level: pSB1AT3-1
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB1AT3_1/annotation1569319/range1569319/1

https://synbiohub.org/public/igem/pSB1AT3_1/annotation1569319/range1569319/1
type: Range
persistent identity: range1569319
display id: range1569319
version: 1
top level: pSB1AT3-1
direction: direction/forward
start: 1091
end: 1091
orientation: inline

ccdA- (rev)
type: SequenceAnnotation
persistent identity: annotation1569259
display id: annotation1569259
version: 1
top level: pSB1AT3-1
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/pSB1AT3_1/annotation1569259/range1569259/1

https://synbiohub.org/public/igem/pSB1AT3_1/annotation1569259/range1569259/1
type: Range
persistent identity: range1569259
display id: range1569259
version: 1
top level: pSB1AT3-1
direction: direction/forward
start: 476
end: 696
orientation: inline

VF2
type: SequenceAnnotation
persistent identity: annotation1569296
display id: annotation1569296
version: 1
top level: pSB1AT3-1
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/pSB1AT3_1/annotation1569296/range1569296/1

https://synbiohub.org/public/igem/pSB1AT3_1/annotation1569296/range1569296/1
type: Range
persistent identity: range1569296
display id: range1569296
version: 1
top level: pSB1AT3-1
direction: direction/forward
start: 4042
end: 4061
orientation: inline

ccdB (rev)
type: SequenceAnnotation
persistent identity: annotation1569258
display id: annotation1569258
version: 1
top level: pSB1AT3-1
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/pSB1AT3_1/annotation1569258/range1569258/1

https://synbiohub.org/public/igem/pSB1AT3_1/annotation1569258/range1569258/1
type: Range
persistent identity: range1569258
display id: range1569258
version: 1
top level: pSB1AT3-1
direction: direction/forward
start: 169
end: 474
orientation: inline

VR
type: SequenceAnnotation
persistent identity: annotation1569306
display id: annotation1569306
version: 1
top level: pSB1AT3-1
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/pSB1AT3_1/annotation1569306/range1569306/1

https://synbiohub.org/public/igem/pSB1AT3_1/annotation1569306/range1569306/1
type: Range
persistent identity: range1569306
display id: range1569306
version: 1
top level: pSB1AT3-1
direction: direction/forward
start: 972
end: 991
orientation: inline

terminator
type: SequenceAnnotation
persistent identity: annotation1569324
display id: annotation1569324
version: 1
top level: pSB1AT3-1
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/pSB1AT3_1/annotation1569324/range1569324/1

https://synbiohub.org/public/igem/pSB1AT3_1/annotation1569324/range1569324/1
type: Range
persistent identity: range1569324
display id: range1569324
version: 1
top level: pSB1AT3-1
direction: direction/forward
start: 90
end: 109
orientation: inline

BB-Pre
type: SequenceAnnotation
persistent identity: annotation1569260
display id: annotation1569260
version: 1
top level: pSB1AT3-1
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/pSB1AT3_1/annotation1569260/range1569260/1

https://synbiohub.org/public/igem/pSB1AT3_1/annotation1569260/range1569260/1
type: Range
persistent identity: range1569260
display id: range1569260
version: 1
top level: pSB1AT3-1
direction: direction/forward
start: 120
end: 135
orientation: inline

APr (rev)
type: SequenceAnnotation
persistent identity: annotation1569235
display id: annotation1569235
version: 1
top level: pSB1AT3-1
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/pSB1AT3_1/annotation1569235/range1569235/1

https://synbiohub.org/public/igem/pSB1AT3_1/annotation1569235/range1569235/1
type: Range
persistent identity: range1569235
display id: range1569235
version: 1
top level: pSB1AT3-1
direction: direction/forward
start: 3139
end: 3999
orientation: inline

BBa_I11004
type: ComponentDefinition
persistent identity: BBa I11004
display id: BBa_I11004
version: 1
was generated by: iGEM to SBOL conversion
description: pZA3PLtet-0luc
created: 2004-08-17T11:00:00Z
modified: 2015-08-31T04:07:30Z
owned by: james
owned by: myers
mutable description: Plasmid with p15A origin of replication, Chloramphenicol resistance, and tet-0 promoter
discontinued: false
dominant: false
experience: experience/None
group u list: _2_
m user id: 0
owner id: 102
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Lutz and Bujard
type: DnaRegion
role: SO:0000155
role: partType/Plasmid
sequence: https://synbiohub.org/public/igem/BBa_I11004_sequence/1

BBa_K394001
type: ComponentDefinition
persistent identity: BBa K394001
display id: BBa_K394001
version: 1
was generated by: iGEM to SBOL conversion
description: Plasmid for Chromosomal Integration in Yeast at His3
created: 2010-10-19T11:00:00Z
modified: 2015-05-08T01:12:21Z
mutable provenance: This part was derived from plasmid pSB1AT3. Two successive rounds of PCR inserted a BbsI cut site and 40 bp homologous region in before the prefix and after the suffix. The following Primers were used: Forward 1 GCACAGAACAATAACCTGCTGGAAACGAAGATAAATCgaagacGATTACTTCGCGTTATGCAGGC Reverse 1 GCATCTTCTCAAATATGCTTCCCAGCCTGCTTATCcttctgAAATTCTGCCTCGTGATACGCC Forward 2 tactagtagcggccgctgcagTCTTAACCCAACTGCACAGAACAATAACCTGCTGGAAACG Reverse 2 ctctagaagcggccgcgaattcTTAGTATTGCTGGCCGCATCTTCTCAAATATGCTTCCCAGCC
owned by: james
owned by: myers
mutable description: This part is designed to allow simple chromosomal integration of BioBrick plasmids. It is based off of the bacterial pSB1AT3 backbone allowing for normal DNA manipulation to occur in bacteria. However the BioBrick cut sites are flanked by homologous regions to the His3 locus in S. cerevisiae. To transform S. cerevisiae the plasmid is cut with BbsI creating two non-sticky ends. The linear fragment then contains His3 homologous sequences at its ends that can be used to chromosomally integrate the BioBrick plasmid into the yeast genome. Transformants can then be selected for using His- medium.
discontinued: false
dominant: false
experience: experience/None
group u list: _504_
m user id: 0
owner id: 6327
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable notes: None
star: false
creator: Brian Landry
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: Tet-R
sequence annotation: VR
sequence annotation: His3 homology
sequence annotation: BbsI Cut Site
sequence annotation: rep (pMB1) (rev)
sequence annotation: BioBrick Prefix
sequence annotation: VF2
sequence annotation: BioBrick Suffix
sequence annotation: AmpR (rev)
sequence annotation: ORI
sequence: https://synbiohub.org/public/igem/BBa_K394001_sequence/1

Tet-R
type: SequenceAnnotation
persistent identity: annotation2102637
display id: annotation2102637
version: 1
top level: BBa_K394001
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K394001/annotation2102637/range2102637/1

https://synbiohub.org/public/igem/BBa_K394001/annotation2102637/range2102637/1
type: Range
persistent identity: range2102637
display id: range2102637
version: 1
top level: BBa_K394001
direction: direction/forward
start: 1127
end: 2317
orientation: inline

VR
type: SequenceAnnotation
persistent identity: annotation2102390
display id: annotation2102390
version: 1
top level: BBa_K394001
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K394001/annotation2102390/range2102390/1

https://synbiohub.org/public/igem/BBa_K394001/annotation2102390/range2102390/1
type: Range
persistent identity: range2102390
display id: range2102390
version: 1
top level: BBa_K394001
direction: direction/reverse
start: 157
end: 176
orientation: inline

His3 homology
type: SequenceAnnotation
persistent identity: annotation2102389
display id: annotation2102389
version: 1
top level: BBa_K394001
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K394001/annotation2102389/range2102389/1

https://synbiohub.org/public/igem/BBa_K394001/annotation2102389/range2102389/1
type: Range
persistent identity: range2102389
display id: range2102389
version: 1
top level: BBa_K394001
direction: direction/forward
start: 22
end: 69
orientation: inline

BbsI Cut Site
type: SequenceAnnotation
persistent identity: annotation2104026
display id: annotation2104026
version: 1
top level: BBa_K394001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K394001/annotation2104026/range2104026/1

https://synbiohub.org/public/igem/BBa_K394001/annotation2104026/range2104026/1
type: Range
persistent identity: range2104026
display id: range2104026
version: 1
top level: BBa_K394001
direction: direction/forward
start: 71
end: 76
orientation: inline

rep (pMB1) (rev)
type: SequenceAnnotation
persistent identity: annotation2102598
display id: annotation2102598
version: 1
top level: BBa_K394001
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K394001/annotation2102598/range2102598/1

https://synbiohub.org/public/igem/BBa_K394001/annotation2102598/range2102598/1
type: Range
persistent identity: range2102598
display id: range2102598
version: 1
top level: BBa_K394001
direction: direction/reverse
start: 261
end: 875
orientation: inline

BioBrick Prefix
type: SequenceAnnotation
persistent identity: annotation2102649
display id: annotation2102649
version: 1
top level: BBa_K394001
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K394001/annotation2102649/range2102649/1

https://synbiohub.org/public/igem/BBa_K394001/annotation2102649/range2102649/1
type: Range
persistent identity: range2102649
display id: range2102649
version: 1
top level: BBa_K394001
direction: direction/forward
start: 3435
end: 3457
orientation: inline

VF2
type: SequenceAnnotation
persistent identity: annotation2102640
display id: annotation2102640
version: 1
top level: BBa_K394001
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K394001/annotation2102640/range2102640/1

https://synbiohub.org/public/igem/BBa_K394001/annotation2102640/range2102640/1
type: Range
persistent identity: range2102640
display id: range2102640
version: 1
top level: BBa_K394001
direction: direction/forward
start: 3307
end: 3326
orientation: inline

BioBrick Suffix
type: SequenceAnnotation
persistent identity: annotation2090942
display id: annotation2090942
version: 1
top level: BBa_K394001
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K394001/annotation2090942/range2090942/1

https://synbiohub.org/public/igem/BBa_K394001/annotation2090942/range2090942/1
type: Range
persistent identity: range2090942
display id: range2090942
version: 1
top level: BBa_K394001
direction: direction/forward
start: 1
end: 21
orientation: inline

BbsI Cut Site
type: SequenceAnnotation
persistent identity: annotation2104028
display id: annotation2104028
version: 1
top level: BBa_K394001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K394001/annotation2104028/range2104028/1

https://synbiohub.org/public/igem/BBa_K394001/annotation2104028/range2104028/1
type: Range
persistent identity: range2104028
display id: range2104028
version: 1
top level: BBa_K394001
direction: direction/reverse
start: 3379
end: 3385
orientation: inline

AmpR (rev)
type: SequenceAnnotation
persistent identity: annotation2102638
display id: annotation2102638
version: 1
top level: BBa_K394001
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K394001/annotation2102638/range2102638/1

https://synbiohub.org/public/igem/BBa_K394001/annotation2102638/range2102638/1
type: Range
persistent identity: range2102638
display id: range2102638
version: 1
top level: BBa_K394001
direction: direction/reverse
start: 2324
end: 3184
orientation: inline

ORI
type: SequenceAnnotation
persistent identity: annotation2102635
display id: annotation2102635
version: 1
top level: BBa_K394001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K394001/annotation2102635/range2102635/1

https://synbiohub.org/public/igem/BBa_K394001/annotation2102635/range2102635/1
type: Range
persistent identity: range2102635
display id: range2102635
version: 1
top level: BBa_K394001
direction: direction/forward
start: 276
end: 276
orientation: inline

His3 homology
type: SequenceAnnotation
persistent identity: annotation2102645
display id: annotation2102645
version: 1
top level: BBa_K394001
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K394001/annotation2102645/range2102645/1

https://synbiohub.org/public/igem/BBa_K394001/annotation2102645/range2102645/1
type: Range
persistent identity: range2102645
display id: range2102645
version: 1
top level: BBa_K394001
direction: direction/forward
start: 3386
end: 3435
orientation: inline

BBa_I11008
type: ComponentDefinition
persistent identity: BBa I11008
display id: BBa_I11008
version: 1
was generated by: iGEM to SBOL conversion
description: pUHS*2Plac/ara-1 plasmid (MCS 1)
created: 2004-08-17T11:00:00Z
modified: 2015-08-31T04:07:30Z
owned by: james
owned by: myers
mutable description: This plasmid has the pSC101* origin of replication, kanamycin resistance marker, and the lac/ara promoter.
discontinued: false
dominant: false
experience: experience/None
group u list: _2_
m user id: 0
owner id: 102
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Lutz and Bujard
type: DnaRegion
role: SO:0000155
role: partType/Plasmid
sequence: https://synbiohub.org/public/igem/BBa_I11008_sequence/1

BBa_I11003
type: ComponentDefinition
persistent identity: BBa I11003
display id: BBa_I11003
version: 1
was generated by: iGEM to SBOL conversion
description: pZS4Int-1 plasmid
created: 2004-08-17T11:00:00Z
modified: 2015-08-31T04:07:30Z
owned by: james
owned by: myers
mutable description: This is a complete plasmid with the pSC101 origin of replication, spectinomycin resistance marker, and lac/ara promoter
discontinued: false
dominant: false
experience: experience/None
group u list: _2_
m user id: 0
owner id: 102
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Lutz and Bujard
type: DnaRegion
role: SO:0000155
role: partType/Plasmid
sequence: https://synbiohub.org/public/igem/BBa_I11003_sequence/1

F plasmid
type: ComponentDefinition
persistent identity: BBa I50000
display id: BBa_I50000
version: 1
was generated by: iGEM to SBOL conversion
description: F plasmid backbone with BioBrick sites removed.
created: 2005-11-16T12:00:00Z
modified: 2015-08-31T04:07:41Z
owned by: james
owned by: myers
mutable description: This is a (near) single copy origin derived from the F plasmid. The naturally occurring BioBricks sites were removed with an intent to preserve the amino acid and codon frequency where possible. Similar to the pSMART VC sequence from Lucigen http://www.lucigen.com/products/Cloning_Systems/CopyRight.html. It is intended to be combined with other vector components (antibiotic resistance marker, multiple cloning site) in order to form a BioBricks vector. Relevant information can be found at http://openwetware.org/wiki/BioBrick_Parts_for_Plasmid_Engineering.
discontinued: false
dominant: false
experience: experience/None
group u list: _41_6_
m user id: 0
owner id: 126
owning group id: 44
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Reshma Shetty
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: sopC(IncD) repeat region
sequence annotation: ori2 (oriS) single copy replication origin
sequence annotation: C to T mutation (eliminates PstI site)
sequence annotation: parB
sequence annotation: DnaA box
sequence annotation: iteron (repeat region)
sequence annotation: sequence derived from F plasmid in GenBank Accession Number AP001918
sequence annotation: parA
sequence annotation: repE
sequence annotation: A to T mutation (eliminates SpeI site)
sequence annotation: A/T region
sequence annotation: C to G mutation (eliminates XbaI site, noncoding region)
sequence: https://synbiohub.org/public/igem/BBa_I50000_sequence/1

sopC(IncD) repeat region
type: SequenceAnnotation
persistent identity: annotation1757395
display id: annotation1757395
version: 1
top level: F plasmid
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I50000/annotation1757395/range1757395/1

https://synbiohub.org/public/igem/BBa_I50000/annotation1757395/range1757395/1
type: Range
persistent identity: range1757395
display id: range1757395
version: 1
top level: F plasmid
direction: direction/reverse
start: 3938
end: 4640
orientation: inline

ori2 (oriS) single copy replication origin
type: SequenceAnnotation
persistent identity: annotation1757409
display id: annotation1757409
version: 1
top level: F plasmid
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I50000/annotation1757409/range1757409/1

https://synbiohub.org/public/igem/BBa_I50000/annotation1757409/range1757409/1
type: Range
persistent identity: range1757409
display id: range1757409
version: 1
top level: F plasmid
direction: direction/reverse
start: 1
end: 290
orientation: inline

C to T mutation (eliminates PstI site)
type: SequenceAnnotation
persistent identity: annotation1757412
display id: annotation1757412
version: 1
top level: F plasmid
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_I50000/annotation1757412/range1757412/1

https://synbiohub.org/public/igem/BBa_I50000/annotation1757412/range1757412/1
type: Range
persistent identity: range1757412
display id: range1757412
version: 1
top level: F plasmid
direction: direction/reverse
start: 1013
end: 1013
orientation: inline

parB
type: SequenceAnnotation
persistent identity: annotation1757405
display id: annotation1757405
version: 1
top level: F plasmid
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_I50000/annotation1757405/range1757405/1

https://synbiohub.org/public/igem/BBa_I50000/annotation1757405/range1757405/1
type: Range
persistent identity: range1757405
display id: range1757405
version: 1
top level: F plasmid
direction: direction/forward
start: 2893
end: 3864
orientation: inline

DnaA box
type: SequenceAnnotation
persistent identity: annotation1757420
display id: annotation1757420
version: 1
top level: F plasmid
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_I50000/annotation1757420/range1757420/1

https://synbiohub.org/public/igem/BBa_I50000/annotation1757420/range1757420/1
type: Range
persistent identity: range1757420
display id: range1757420
version: 1
top level: F plasmid
direction: direction/forward
start: 81
end: 88
orientation: inline

iteron (repeat region)
type: SequenceAnnotation
persistent identity: annotation1757419
display id: annotation1757419
version: 1
top level: F plasmid
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I50000/annotation1757419/range1757419/1

https://synbiohub.org/public/igem/BBa_I50000/annotation1757419/range1757419/1
type: Range
persistent identity: range1757419
display id: range1757419
version: 1
top level: F plasmid
direction: direction/forward
start: 268
end: 287
orientation: inline

sequence derived from F plasmid in GenBank Accession Number AP001918
type: SequenceAnnotation
persistent identity: annotation1757421
display id: annotation1757421
version: 1
top level: F plasmid
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I50000/annotation1757421/range1757421/1

https://synbiohub.org/public/igem/BBa_I50000/annotation1757421/range1757421/1
type: Range
persistent identity: range1757421
display id: range1757421
version: 1
top level: F plasmid
direction: direction/reverse
start: 1720
end: 4640
orientation: inline

DnaA box
type: SequenceAnnotation
persistent identity: annotation1757414
display id: annotation1757414
version: 1
top level: F plasmid
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_I50000/annotation1757414/range1757414/1

https://synbiohub.org/public/igem/BBa_I50000/annotation1757414/range1757414/1
type: Range
persistent identity: range1757414
display id: range1757414
version: 1
top level: F plasmid
direction: direction/forward
start: 89
end: 97
orientation: inline

parA
type: SequenceAnnotation
persistent identity: annotation1757406
display id: annotation1757406
version: 1
top level: F plasmid
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_I50000/annotation1757406/range1757406/1

https://synbiohub.org/public/igem/BBa_I50000/annotation1757406/range1757406/1
type: Range
persistent identity: range1757406
display id: range1757406
version: 1
top level: F plasmid
direction: direction/forward
start: 1718
end: 2893
orientation: inline

iteron (repeat region)
type: SequenceAnnotation
persistent identity: annotation1757417
display id: annotation1757417
version: 1
top level: F plasmid
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I50000/annotation1757417/range1757417/1

https://synbiohub.org/public/igem/BBa_I50000/annotation1757417/range1757417/1
type: Range
persistent identity: range1757417
display id: range1757417
version: 1
top level: F plasmid
direction: direction/forward
start: 213
end: 232
orientation: inline

iteron (repeat region)
type: SequenceAnnotation
persistent identity: annotation1757416
display id: annotation1757416
version: 1
top level: F plasmid
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I50000/annotation1757416/range1757416/1

https://synbiohub.org/public/igem/BBa_I50000/annotation1757416/range1757416/1
type: Range
persistent identity: range1757416
display id: range1757416
version: 1
top level: F plasmid
direction: direction/forward
start: 192
end: 211
orientation: inline

repE
type: SequenceAnnotation
persistent identity: annotation1757408
display id: annotation1757408
version: 1
top level: F plasmid
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_I50000/annotation1757408/range1757408/1

https://synbiohub.org/public/igem/BBa_I50000/annotation1757408/range1757408/1
type: Range
persistent identity: range1757408
display id: range1757408
version: 1
top level: F plasmid
direction: direction/forward
start: 384
end: 1139
orientation: inline

iteron (repeat region)
type: SequenceAnnotation
persistent identity: annotation1757418
display id: annotation1757418
version: 1
top level: F plasmid
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I50000/annotation1757418/range1757418/1

https://synbiohub.org/public/igem/BBa_I50000/annotation1757418/range1757418/1
type: Range
persistent identity: range1757418
display id: range1757418
version: 1
top level: F plasmid
direction: direction/forward
start: 246
end: 265
orientation: inline

A to T mutation (eliminates SpeI site)
type: SequenceAnnotation
persistent identity: annotation1757411
display id: annotation1757411
version: 1
top level: F plasmid
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_I50000/annotation1757411/range1757411/1

https://synbiohub.org/public/igem/BBa_I50000/annotation1757411/range1757411/1
type: Range
persistent identity: range1757411
display id: range1757411
version: 1
top level: F plasmid
direction: direction/reverse
start: 3711
end: 3711
orientation: inline

sequence derived from F plasmid in GenBank Accession Number AP001918
type: SequenceAnnotation
persistent identity: annotation1757407
display id: annotation1757407
version: 1
top level: F plasmid
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I50000/annotation1757407/range1757407/1

https://synbiohub.org/public/igem/BBa_I50000/annotation1757407/range1757407/1
type: Range
persistent identity: range1757407
display id: range1757407
version: 1
top level: F plasmid
direction: direction/reverse
start: 1
end: 1390
orientation: inline

C to T mutation (eliminates PstI site)
type: SequenceAnnotation
persistent identity: annotation1757410
display id: annotation1757410
version: 1
top level: F plasmid
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_I50000/annotation1757410/range1757410/1

https://synbiohub.org/public/igem/BBa_I50000/annotation1757410/range1757410/1
type: Range
persistent identity: range1757410
display id: range1757410
version: 1
top level: F plasmid
direction: direction/reverse
start: 2553
end: 2553
orientation: inline

A/T region
type: SequenceAnnotation
persistent identity: annotation1757415
display id: annotation1757415
version: 1
top level: F plasmid
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I50000/annotation1757415/range1757415/1

https://synbiohub.org/public/igem/BBa_I50000/annotation1757415/range1757415/1
type: Range
persistent identity: range1757415
display id: range1757415
version: 1
top level: F plasmid
direction: direction/forward
start: 101
end: 147
orientation: inline

C to G mutation (eliminates XbaI site, noncoding region)
type: SequenceAnnotation
persistent identity: annotation1757413
display id: annotation1757413
version: 1
top level: F plasmid
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_I50000/annotation1757413/range1757413/1

https://synbiohub.org/public/igem/BBa_I50000/annotation1757413/range1757413/1
type: Range
persistent identity: range1757413
display id: range1757413
version: 1
top level: F plasmid
direction: direction/reverse
start: 183
end: 183
orientation: inline

BBa_I11005
type: ComponentDefinition
persistent identity: BBa I11005
display id: BBa_I11005
version: 1
was generated by: iGEM to SBOL conversion
description: pZE1PA1lacO plasmid
created: 2004-08-17T11:00:00Z
modified: 2015-08-31T04:07:30Z
owned by: james
owned by: myers
mutable description: Plasmid with ColE1 origin of replication, amp resistance marker, and A1lac0 promoter with multiple cloning sites
discontinued: false
dominant: false
experience: experience/None
group u list: _2_
m user id: 0
owner id: 102
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Lutz and Bujard
type: DnaRegion
role: SO:0000155
role: partType/Plasmid
sequence: https://synbiohub.org/public/igem/BBa_I11005_sequence/1

pSB1AK3-1
type: ComponentDefinition
persistent identity: pSB1AK3 1
display id: pSB1AK3_1
version: 1
was generated by: iGEM to SBOL conversion
description: PSB1AK3 with ccdB insert
created: 2005-06-05T11:00:00Z
modified: 2015-05-08T01:14:55Z
owned by: james
owned by: myers
mutable description: Target plasmid for three antibiotic assembly. The ccdB insert prevents growth of the unmodified plasmid in normal cloning strains, allowing selection of only correctly assembled recombinants.
discontinued: true
dominant: false
experience: experience/Works
group u list: _42_
m user id: 0
owner id: 6
owning group id: 42
sample status: Discontinued
status: status/Deleted
bookmark: false
star: false
creator: Tom Knight
type: DnaRegion
role: SO:0000155
role: partType/Plasmid
sequence annotation: Vr
sequence annotation: ccdB (rev)
sequence annotation: kanR (rev)
sequence annotation: VF2
sequence annotation: BB-Post
sequence annotation: terminator
sequence annotation: ccdA- (rev)
sequence annotation: ORI
sequence annotation: ampR (rev)
sequence annotation: ccD Operon (rev)
sequence annotation: BB-Pre
sequence annotation: rep (pMB1) (rev)
sequence annotation: Vf
sequence: https://synbiohub.org/public/igem/pSB1AK3_1_sequence/1

Vr
type: SequenceAnnotation
persistent identity: annotation1569178
display id: annotation1569178
version: 1
top level: pSB1AK3-1
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/pSB1AK3_1/annotation1569178/range1569178/1

https://synbiohub.org/public/igem/pSB1AK3_1/annotation1569178/range1569178/1
type: Range
persistent identity: range1569178
display id: range1569178
version: 1
top level: pSB1AK3-1
direction: direction/forward
start: 972
end: 991
orientation: inline

ccdB (rev)
type: SequenceAnnotation
persistent identity: annotation1569166
display id: annotation1569166
version: 1
top level: pSB1AK3-1
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/pSB1AK3_1/annotation1569166/range1569166/1

https://synbiohub.org/public/igem/pSB1AK3_1/annotation1569166/range1569166/1
type: Range
persistent identity: range1569166
display id: range1569166
version: 1
top level: pSB1AK3-1
direction: direction/forward
start: 169
end: 474
orientation: inline

kanR (rev)
type: SequenceAnnotation
persistent identity: annotation1569140
display id: annotation1569140
version: 1
top level: pSB1AK3-1
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/pSB1AK3_1/annotation1569140/range1569140/1

https://synbiohub.org/public/igem/pSB1AK3_1/annotation1569140/range1569140/1
type: Range
persistent identity: range1569140
display id: range1569140
version: 1
top level: pSB1AK3-1
direction: direction/forward
start: 1912
end: 2727
orientation: inline

VF2
type: SequenceAnnotation
persistent identity: annotation1780824
display id: annotation1780824
version: 1
top level: pSB1AK3-1
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/pSB1AK3_1/annotation1780824/range1780824/1

https://synbiohub.org/public/igem/pSB1AK3_1/annotation1780824/range1780824/1
type: Range
persistent identity: range1780824
display id: range1780824
version: 1
top level: pSB1AK3-1
direction: direction/forward
start: 1
end: 20
orientation: inline

BB-Post
type: SequenceAnnotation
persistent identity: annotation1569176
display id: annotation1569176
version: 1
top level: pSB1AK3-1
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/pSB1AK3_1/annotation1569176/range1569176/1

https://synbiohub.org/public/igem/pSB1AK3_1/annotation1569176/range1569176/1
type: Range
persistent identity: range1569176
display id: range1569176
version: 1
top level: pSB1AK3-1
direction: direction/forward
start: 818
end: 836
orientation: inline

terminator
type: SequenceAnnotation
persistent identity: annotation1569183
display id: annotation1569183
version: 1
top level: pSB1AK3-1
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/pSB1AK3_1/annotation1569183/range1569183/1

https://synbiohub.org/public/igem/pSB1AK3_1/annotation1569183/range1569183/1
type: Range
persistent identity: range1569183
display id: range1569183
version: 1
top level: pSB1AK3-1
direction: direction/forward
start: 845
end: 879
orientation: inline

ccdA- (rev)
type: SequenceAnnotation
persistent identity: annotation1569167
display id: annotation1569167
version: 1
top level: pSB1AK3-1
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/pSB1AK3_1/annotation1569167/range1569167/1

https://synbiohub.org/public/igem/pSB1AK3_1/annotation1569167/range1569167/1
type: Range
persistent identity: range1569167
display id: range1569167
version: 1
top level: pSB1AK3-1
direction: direction/forward
start: 476
end: 696
orientation: inline

ORI
type: SequenceAnnotation
persistent identity: annotation1569184
display id: annotation1569184
version: 1
top level: pSB1AK3-1
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB1AK3_1/annotation1569184/range1569184/1

https://synbiohub.org/public/igem/pSB1AK3_1/annotation1569184/range1569184/1
type: Range
persistent identity: range1569184
display id: range1569184
version: 1
top level: pSB1AK3-1
direction: direction/forward
start: 1091
end: 1091
orientation: inline

terminator
type: SequenceAnnotation
persistent identity: annotation1569179
display id: annotation1569179
version: 1
top level: pSB1AK3-1
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/pSB1AK3_1/annotation1569179/range1569179/1

https://synbiohub.org/public/igem/pSB1AK3_1/annotation1569179/range1569179/1
type: Range
persistent identity: range1569179
display id: range1569179
version: 1
top level: pSB1AK3-1
direction: direction/forward
start: 90
end: 109
orientation: inline

ampR (rev)
type: SequenceAnnotation
persistent identity: annotation1569165
display id: annotation1569165
version: 1
top level: pSB1AK3-1
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/pSB1AK3_1/annotation1569165/range1569165/1

https://synbiohub.org/public/igem/pSB1AK3_1/annotation1569165/range1569165/1
type: Range
persistent identity: range1569165
display id: range1569165
version: 1
top level: pSB1AK3-1
direction: direction/forward
start: 2882
end: 3742
orientation: inline

ccD Operon (rev)
type: SequenceAnnotation
persistent identity: annotation1569185
display id: annotation1569185
version: 1
top level: pSB1AK3-1
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB1AK3_1/annotation1569185/range1569185/1

https://synbiohub.org/public/igem/pSB1AK3_1/annotation1569185/range1569185/1
type: Range
persistent identity: range1569185
display id: range1569185
version: 1
top level: pSB1AK3-1
direction: direction/forward
start: 140
end: 816
orientation: inline

BB-Pre
type: SequenceAnnotation
persistent identity: annotation1569175
display id: annotation1569175
version: 1
top level: pSB1AK3-1
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/pSB1AK3_1/annotation1569175/range1569175/1

https://synbiohub.org/public/igem/pSB1AK3_1/annotation1569175/range1569175/1
type: Range
persistent identity: range1569175
display id: range1569175
version: 1
top level: pSB1AK3-1
direction: direction/forward
start: 120
end: 135
orientation: inline

rep (pMB1) (rev)
type: SequenceAnnotation
persistent identity: annotation1569174
display id: annotation1569174
version: 1
top level: pSB1AK3-1
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB1AK3_1/annotation1569174/range1569174/1

https://synbiohub.org/public/igem/pSB1AK3_1/annotation1569174/range1569174/1
type: Range
persistent identity: range1569174
display id: range1569174
version: 1
top level: pSB1AK3-1
direction: direction/forward
start: 1076
end: 1690
orientation: inline

Vf
type: SequenceAnnotation
persistent identity: annotation1569177
display id: annotation1569177
version: 1
top level: pSB1AK3-1
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/pSB1AK3_1/annotation1569177/range1569177/1

https://synbiohub.org/public/igem/pSB1AK3_1/annotation1569177/range1569177/1
type: Range
persistent identity: range1569177
display id: range1569177
version: 1
top level: pSB1AK3-1
direction: direction/forward
start: 3785
end: 3804
orientation: inline

BBa_K283042
type: ComponentDefinition
persistent identity: BBa K283042
display id: BBa_K283042
version: 1
was generated by: iGEM to SBOL conversion
description: pTet-rbs-rhlR
created: 2009-10-18T11:00:00Z
modified: 2015-05-08T01:11:48Z
mutable provenance: From the registry
owned by: james
owned by: myers
mutable description: cI coding region on vector J61002
discontinued: false
dominant: false
experience: experience/None
group u list: _383_
m user id: 0
owner id: 5494
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: BBa_K283042
star: false
creator: W Ling , Nelson Chu, Shi Lei
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_C0071
component: BBa_B0034
component: BBa_R0040
sequence annotation: BBa_C0071
sequence annotation: BBa_R0040
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K283042_sequence/1

BBa_C0071
type: Component
persistent identity: component2246852
display id: component2246852
version: 1
top level: BBa_K283042
access: public
definition: rhlR

BBa_B0034
type: Component
persistent identity: component2246848
display id: component2246848
version: 1
top level: BBa_K283042
access: public
definition: BBa_B0034

BBa_R0040
type: Component
persistent identity: component2246842
display id: component2246842
version: 1
top level: BBa_K283042
access: public
definition: p(tetR)

BBa_C0071
type: SequenceAnnotation
persistent identity: annotation2246852
display id: annotation2246852
version: 1
top level: BBa_K283042
location: https://synbiohub.org/public/igem/BBa_K283042/annotation2246852/range2246852/1
component: BBa_C0071

https://synbiohub.org/public/igem/BBa_K283042/annotation2246852/range2246852/1
type: Range
persistent identity: range2246852
display id: range2246852
version: 1
top level: BBa_K283042
direction: direction/reverse
start: 81
end: 867
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation2246842
display id: annotation2246842
version: 1
top level: BBa_K283042
location: https://synbiohub.org/public/igem/BBa_K283042/annotation2246842/range2246842/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_K283042/annotation2246842/range2246842/1
type: Range
persistent identity: range2246842
display id: range2246842
version: 1
top level: BBa_K283042
direction: direction/forward
start: 1
end: 54
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2246848
display id: annotation2246848
version: 1
top level: BBa_K283042
location: https://synbiohub.org/public/igem/BBa_K283042/annotation2246848/range2246848/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K283042/annotation2246848/range2246848/1
type: Range
persistent identity: range2246848
display id: range2246848
version: 1
top level: BBa_K283042
direction: direction/reverse
start: 63
end: 74
orientation: inline

rhlR
type: ComponentDefinition
persistent identity: BBa C0071
display id: BBa_C0071
version: 1
was generated by: iGEM to SBOL conversion
description: rhlR repressor/activator from P. aeruginosa PA3477 (+LVA)
created: 2004-01-26T12:00:00Z
modified: 2015-08-31T04:07:23Z
mutable provenance: P. aeruginosa PA3477
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcriptional regulator, in complex with N-butyryl-HSL, RhlR binds to the Rhl promoter
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: (1/28/04 Edit Jamboree) (0) No BB sites (1) ATG (2) TAATAA (3) Blast checks out (4) No codon changes (5) Maybe chassis compatability (check by experiment) (6) Codon usage not optimal (but looks OK) (7) ssrA tag added
star: true
creator: Debra Lin, Srini Devadas, David Gray, Ronny Krashinsky, and Chris Zheng Liu, Boopers
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: rhlR
sequence annotation: Help:Barcodes
sequence annotation: LVA
sequence: https://synbiohub.org/public/igem/BBa_C0071_sequence/1

rhlR
type: SequenceAnnotation
persistent identity: annotation301192
display id: annotation301192
version: 1
top level: rhlR
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_C0071/annotation301192/range301192/1

https://synbiohub.org/public/igem/BBa_C0071/annotation301192/range301192/1
type: Range
persistent identity: range301192
display id: range301192
version: 1
top level: rhlR
direction: direction/forward
start: 1
end: 723
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213995
display id: annotation2213995
version: 1
top level: rhlR
role: SO:0000807
role: feature/barcode
location: https://synbiohub.org/public/igem/BBa_C0071/annotation2213995/range2213995/1

https://synbiohub.org/public/igem/BBa_C0071/annotation2213995/range2213995/1
type: Range
persistent identity: range2213995
display id: range2213995
version: 1
top level: rhlR
direction: direction/forward
start: 763
end: 787
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation306523
display id: annotation306523
version: 1
top level: rhlR
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_C0071/annotation306523/range306523/1

https://synbiohub.org/public/igem/BBa_C0071/annotation306523/range306523/1
type: Range
persistent identity: range306523
display id: range306523
version: 1
top level: rhlR
direction: direction/forward
start: 724
end: 756
orientation: inline

BBa_K1976001
type: ComponentDefinition
persistent identity: BBa K1976001
display id: BBa_K1976001
version: 1
was generated by: iGEM to SBOL conversion
description: λ-Integrase
created: 2016-10-13T11:00:00Z
modified: 2016-10-19T05:26:58Z
mutable provenance: .
owned by: james
owned by: myers
mutable description: Helping plasmid for genomic integration
discontinued: false
dominant: false
experience: experience/None
group u list: _2443_
m user id: 30857
owner id: 26981
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: .
star: false
creator: Thomas Dohmen, Milana Kremenovic, Yannick Kristiansen, Tim Maier
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: J23101
sequence annotation: B0034
sequence annotation: lambda-INT
sequence: https://synbiohub.org/public/igem/BBa_K1976001_sequence/1

J23101
type: SequenceAnnotation
persistent identity: annotation2517065
display id: annotation2517065
version: 1
top level: BBa_K1976001
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1976001/annotation2517065/range2517065/1

https://synbiohub.org/public/igem/BBa_K1976001/annotation2517065/range2517065/1
type: Range
persistent identity: range2517065
display id: range2517065
version: 1
top level: BBa_K1976001
direction: direction/forward
start: 1
end: 35
orientation: inline

B0034
type: SequenceAnnotation
persistent identity: annotation2517066
display id: annotation2517066
version: 1
top level: BBa_K1976001
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1976001/annotation2517066/range2517066/1

https://synbiohub.org/public/igem/BBa_K1976001/annotation2517066/range2517066/1
type: Range
persistent identity: range2517066
display id: range2517066
version: 1
top level: BBa_K1976001
direction: direction/forward
start: 42
end: 53
orientation: inline

lambda-INT
type: SequenceAnnotation
persistent identity: annotation2517067
display id: annotation2517067
version: 1
top level: BBa_K1976001
role: feature/protein
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1976001/annotation2517067/range2517067/1

https://synbiohub.org/public/igem/BBa_K1976001/annotation2517067/range2517067/1
type: Range
persistent identity: range2517067
display id: range2517067
version: 1
top level: BBa_K1976001
direction: direction/forward
start: 54
end: 1124
orientation: inline

BBa_I50010
type: ComponentDefinition
persistent identity: BBa I50010
display id: BBa_I50010
version: 1
was generated by: iGEM to SBOL conversion
description: oriV origin
created: 2005-12-03T12:00:00Z
modified: 2015-08-31T04:07:41Z
owned by: james
owned by: myers
mutable description: oriV origin which requires the presence of the RK2-encoded TrfA replication protein (resident on the genome of a host-strain) to be functional. Vector copy number will be 20-50 if this replication origin is active. For this origin to be functional, the strain background must contain the trfA gene. Generally the appropriate strain has a copy-up TrfA mutant protein controlled by the ParaBAD promoter. Thus, L-arabinose can be added to the media to induce expression of the TrfA protein and enable replication at the oriV origin. The origin will not be functional in host strains without this protein. Generally, this origin is used as a secondary, inducible copy number origin in F plasmid based vectors. It allows conditional amplication of the vector copy number when in the appropriate host strain. Similar to part of the pSMART VC sequence from Lucigen http://www.lucigen.com/products/Cloning_Systems/CopyRight.html. It is intended to be combined with other vector components (antibiotic resistance marker, multiple cloning site, a single copy origin like BBa_I50000) in order to form a BioBricks vector. Relevant information can be found at http://openwetware.org/wiki/BioBrick_Parts_for_Plasmid_Engineering.
discontinued: false
dominant: false
group u list: _41_6_
m user id: 0
owner id: 126
owning group id: 44
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000155
role: partType/Plasmid
sequence annotation: iteron? (repeat unit)
sequence annotation: iteron (repeat unit)
sequence: https://synbiohub.org/public/igem/BBa_I50010_sequence/1

iteron? (repeat unit)
type: SequenceAnnotation
persistent identity: annotation1757863
display id: annotation1757863
version: 1
top level: BBa_I50010
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I50010/annotation1757863/range1757863/1

https://synbiohub.org/public/igem/BBa_I50010/annotation1757863/range1757863/1
type: Range
persistent identity: range1757863
display id: range1757863
version: 1
top level: BBa_I50010
direction: direction/forward
start: 222
end: 238
orientation: inline

iteron? (repeat unit)
type: SequenceAnnotation
persistent identity: annotation1757865
display id: annotation1757865
version: 1
top level: BBa_I50010
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I50010/annotation1757865/range1757865/1

https://synbiohub.org/public/igem/BBa_I50010/annotation1757865/range1757865/1
type: Range
persistent identity: range1757865
display id: range1757865
version: 1
top level: BBa_I50010
direction: direction/forward
start: 178
end: 193
orientation: inline

iteron (repeat unit)
type: SequenceAnnotation
persistent identity: annotation1757858
display id: annotation1757858
version: 1
top level: BBa_I50010
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I50010/annotation1757858/range1757858/1

https://synbiohub.org/public/igem/BBa_I50010/annotation1757858/range1757858/1
type: Range
persistent identity: range1757858
display id: range1757858
version: 1
top level: BBa_I50010
direction: direction/forward
start: 199
end: 216
orientation: inline

iteron (repeat unit)
type: SequenceAnnotation
persistent identity: annotation1757861
display id: annotation1757861
version: 1
top level: BBa_I50010
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I50010/annotation1757861/range1757861/1

https://synbiohub.org/public/igem/BBa_I50010/annotation1757861/range1757861/1
type: Range
persistent identity: range1757861
display id: range1757861
version: 1
top level: BBa_I50010
direction: direction/forward
start: 406
end: 420
orientation: inline

iteron (repeat unit)
type: SequenceAnnotation
persistent identity: annotation1757860
display id: annotation1757860
version: 1
top level: BBa_I50010
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I50010/annotation1757860/range1757860/1

https://synbiohub.org/public/igem/BBa_I50010/annotation1757860/range1757860/1
type: Range
persistent identity: range1757860
display id: range1757860
version: 1
top level: BBa_I50010
direction: direction/forward
start: 461
end: 477
orientation: inline

iteron? (repeat unit)
type: SequenceAnnotation
persistent identity: annotation1757864
display id: annotation1757864
version: 1
top level: BBa_I50010
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I50010/annotation1757864/range1757864/1

https://synbiohub.org/public/igem/BBa_I50010/annotation1757864/range1757864/1
type: Range
persistent identity: range1757864
display id: range1757864
version: 1
top level: BBa_I50010
direction: direction/forward
start: 439
end: 456
orientation: inline

iteron (repeat unit)
type: SequenceAnnotation
persistent identity: annotation1757859
display id: annotation1757859
version: 1
top level: BBa_I50010
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I50010/annotation1757859/range1757859/1

https://synbiohub.org/public/igem/BBa_I50010/annotation1757859/range1757859/1
type: Range
persistent identity: range1757859
display id: range1757859
version: 1
top level: BBa_I50010
direction: direction/forward
start: 244
end: 261
orientation: inline

iteron? (repeat unit)
type: SequenceAnnotation
persistent identity: annotation1757862
display id: annotation1757862
version: 1
top level: BBa_I50010
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I50010/annotation1757862/range1757862/1

https://synbiohub.org/public/igem/BBa_I50010/annotation1757862/range1757862/1
type: Range
persistent identity: range1757862
display id: range1757862
version: 1
top level: BBa_I50010
direction: direction/forward
start: 266
end: 283
orientation: inline

BBa_K1976004
type: ComponentDefinition
persistent identity: BBa K1976004
display id: BBa_K1976004
version: 1
was generated by: iGEM to SBOL conversion
description: λ-attP site flanked with two bidirectional terminators
created: 2016-10-13T11:00:00Z
modified: 2016-10-19T06:40:45Z
mutable provenance: idt
owned by: james
owned by: myers
mutable description: mVenus-Repressor-Anderson Promoter
discontinued: false
dominant: false
experience: experience/None
group u list: _2443_
m user id: 30857
owner id: 26981
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: .
star: false
creator: Thomas Dohmen, Milana Kremenovic, Yannick Kristiansen, Patrick Kunzmann, Tim Maier
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: Bidirectional Terminator
sequence annotation: Lambda-attP site
sequence: https://synbiohub.org/public/igem/BBa_K1976004_sequence/1

Bidirectional Terminator
type: SequenceAnnotation
persistent identity: annotation2520804
display id: annotation2520804
version: 1
top level: BBa_K1976004
role: feature/dna
role: SO:0000110
location: https://synbiohub.org/public/igem/BBa_K1976004/annotation2520804/range2520804/1

https://synbiohub.org/public/igem/BBa_K1976004/annotation2520804/range2520804/1
type: Range
persistent identity: range2520804
display id: range2520804
version: 1
top level: BBa_K1976004
direction: direction/reverse
start: 267
end: 300
orientation: inline

Bidirectional Terminator
type: SequenceAnnotation
persistent identity: annotation2520802
display id: annotation2520802
version: 1
top level: BBa_K1976004
role: feature/dna
role: SO:0000110
location: https://synbiohub.org/public/igem/BBa_K1976004/annotation2520802/range2520802/1

https://synbiohub.org/public/igem/BBa_K1976004/annotation2520802/range2520802/1
type: Range
persistent identity: range2520802
display id: range2520802
version: 1
top level: BBa_K1976004
direction: direction/forward
start: 1
end: 34
orientation: inline

Lambda-attP site
type: SequenceAnnotation
persistent identity: annotation2520803
display id: annotation2520803
version: 1
top level: BBa_K1976004
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1976004/annotation2520803/range2520803/1

https://synbiohub.org/public/igem/BBa_K1976004/annotation2520803/range2520803/1
type: Range
persistent identity: range2520803
display id: range2520803
version: 1
top level: BBa_K1976004
direction: direction/forward
start: 35
end: 266
orientation: inline

BBa_K394002
type: ComponentDefinition
persistent identity: BBa K394002
display id: BBa_K394002
version: 1
was generated by: iGEM to SBOL conversion
description: Plasmid for Chromosomal Integration in Yeast at Ura3
created: 2010-10-19T11:00:00Z
modified: 2015-05-08T01:12:21Z
mutable provenance: This part was derived from plasmid pSB1AT3. Two successive rounds of PCR inserted a BbsI cut site and 40 bp homologous region in before the prefix and after the suffix. The following Primers were used: Forward 1 GCACAGAACAATAACCTGCTGGAAACGAAGATAAATCgaagacGATTACTTCGCGTTATGCAGGC Reverse 1 GCATCTTCTCAAATATGCTTCCCAGCCTGCTTATCcttctgAAATTCTGCCTCGTGATACGCC Forward 2 tactagtagcggccgctgcagTCTTAACCCAACTGCACAGAACAATAACCTGCTGGAAACG Reverse 2 ctctagaagcggccgcgaattcTTAGTATTGCTGGCCGCATCTTCTCAAATATGCTTCCCAGCC
owned by: james
owned by: myers
mutable description: This part is designed to allow simple chromosomal integration of BioBrick plasmids. It is based off of the bacterial pSB1AT3 backbone allowing for normal DNA manipulation to occur in bacteria. However the BioBrick cut sites are flanked by homologous regions to the Ura3 locus in S. cerevisiae. To transform S. cerevisiae the plasmid is cut with BbsI creating two non-sticky ends. The linear fragment then contains Ura3 homologous sequences at its ends that can be used to chromosomally integrate the BioBrick plasmid into the yeast genome. Transformants can then be selected for using Ura- medium.
discontinued: false
dominant: false
group u list: _504_
m user id: 0
owner id: 6327
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: None
star: false
creator: Brian Landry
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: AmpR (rev)
sequence annotation: ORI
sequence annotation: Ura3 homology
sequence annotation: Tet-R
sequence annotation: BbsI Cut Site
sequence annotation: rep (pMB1) (rev)
sequence annotation: BioBrick Suffix
sequence annotation: VR
sequence annotation: VF2
sequence annotation: BioBrick Prefix
sequence: https://synbiohub.org/public/igem/BBa_K394002_sequence/1

AmpR (rev)
type: SequenceAnnotation
persistent identity: annotation2102742
display id: annotation2102742
version: 1
top level: BBa_K394002
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K394002/annotation2102742/range2102742/1

https://synbiohub.org/public/igem/BBa_K394002/annotation2102742/range2102742/1
type: Range
persistent identity: range2102742
display id: range2102742
version: 1
top level: BBa_K394002
direction: direction/reverse
start: 2324
end: 3184
orientation: inline

ORI
type: SequenceAnnotation
persistent identity: annotation2102740
display id: annotation2102740
version: 1
top level: BBa_K394002
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K394002/annotation2102740/range2102740/1

https://synbiohub.org/public/igem/BBa_K394002/annotation2102740/range2102740/1
type: Range
persistent identity: range2102740
display id: range2102740
version: 1
top level: BBa_K394002
direction: direction/forward
start: 276
end: 276
orientation: inline

Ura3 homology
type: SequenceAnnotation
persistent identity: annotation2102744
display id: annotation2102744
version: 1
top level: BBa_K394002
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K394002/annotation2102744/range2102744/1

https://synbiohub.org/public/igem/BBa_K394002/annotation2102744/range2102744/1
type: Range
persistent identity: range2102744
display id: range2102744
version: 1
top level: BBa_K394002
direction: direction/forward
start: 3387
end: 3435
orientation: inline

Tet-R
type: SequenceAnnotation
persistent identity: annotation2102741
display id: annotation2102741
version: 1
top level: BBa_K394002
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K394002/annotation2102741/range2102741/1

https://synbiohub.org/public/igem/BBa_K394002/annotation2102741/range2102741/1
type: Range
persistent identity: range2102741
display id: range2102741
version: 1
top level: BBa_K394002
direction: direction/forward
start: 1127
end: 2317
orientation: inline

BbsI Cut Site
type: SequenceAnnotation
persistent identity: annotation2103940
display id: annotation2103940
version: 1
top level: BBa_K394002
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K394002/annotation2103940/range2103940/1

https://synbiohub.org/public/igem/BBa_K394002/annotation2103940/range2103940/1
type: Range
persistent identity: range2103940
display id: range2103940
version: 1
top level: BBa_K394002
direction: direction/reverse
start: 3381
end: 3386
orientation: inline

rep (pMB1) (rev)
type: SequenceAnnotation
persistent identity: annotation2102717
display id: annotation2102717
version: 1
top level: BBa_K394002
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K394002/annotation2102717/range2102717/1

https://synbiohub.org/public/igem/BBa_K394002/annotation2102717/range2102717/1
type: Range
persistent identity: range2102717
display id: range2102717
version: 1
top level: BBa_K394002
direction: direction/reverse
start: 261
end: 875
orientation: inline

BioBrick Suffix
type: SequenceAnnotation
persistent identity: annotation2102714
display id: annotation2102714
version: 1
top level: BBa_K394002
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K394002/annotation2102714/range2102714/1

https://synbiohub.org/public/igem/BBa_K394002/annotation2102714/range2102714/1
type: Range
persistent identity: range2102714
display id: range2102714
version: 1
top level: BBa_K394002
direction: direction/forward
start: 1
end: 21
orientation: inline

BbsI Cut Site
type: SequenceAnnotation
persistent identity: annotation2104024
display id: annotation2104024
version: 1
top level: BBa_K394002
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K394002/annotation2104024/range2104024/1

https://synbiohub.org/public/igem/BBa_K394002/annotation2104024/range2104024/1
type: Range
persistent identity: range2104024
display id: range2104024
version: 1
top level: BBa_K394002
direction: direction/forward
start: 72
end: 77
orientation: inline

VR
type: SequenceAnnotation
persistent identity: annotation2102716
display id: annotation2102716
version: 1
top level: BBa_K394002
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K394002/annotation2102716/range2102716/1

https://synbiohub.org/public/igem/BBa_K394002/annotation2102716/range2102716/1
type: Range
persistent identity: range2102716
display id: range2102716
version: 1
top level: BBa_K394002
direction: direction/reverse
start: 157
end: 176
orientation: inline

VF2
type: SequenceAnnotation
persistent identity: annotation2102743
display id: annotation2102743
version: 1
top level: BBa_K394002
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K394002/annotation2102743/range2102743/1

https://synbiohub.org/public/igem/BBa_K394002/annotation2102743/range2102743/1
type: Range
persistent identity: range2102743
display id: range2102743
version: 1
top level: BBa_K394002
direction: direction/forward
start: 3307
end: 3326
orientation: inline

BioBrick Prefix
type: SequenceAnnotation
persistent identity: annotation2102745
display id: annotation2102745
version: 1
top level: BBa_K394002
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K394002/annotation2102745/range2102745/1

https://synbiohub.org/public/igem/BBa_K394002/annotation2102745/range2102745/1
type: Range
persistent identity: range2102745
display id: range2102745
version: 1
top level: BBa_K394002
direction: direction/forward
start: 3435
end: 3457
orientation: inline

Ura3 homology
type: SequenceAnnotation
persistent identity: annotation2102715
display id: annotation2102715
version: 1
top level: BBa_K394002
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K394002/annotation2102715/range2102715/1

https://synbiohub.org/public/igem/BBa_K394002/annotation2102715/range2102715/1
type: Range
persistent identity: range2102715
display id: range2102715
version: 1
top level: BBa_K394002
direction: direction/forward
start: 22
end: 69
orientation: inline

pSB1AC3-1
type: ComponentDefinition
persistent identity: pSB1AC3 1
display id: pSB1AC3_1
version: 1
was generated by: iGEM to SBOL conversion
description: pSB1AC3 with ccdB insert
created: 2005-06-06T11:00:00Z
modified: 2015-05-08T01:14:55Z
owned by: james
owned by: myers
mutable description: Target plasmid for three antibiotic assembly. Chloramphenicol resistant. Contains an insert having a constitutive promoter and the ccdB coding region. ccdB protein is lethal in normal cloning strains. Grown in the special strain DB3.1, which is ccdB resistant.
discontinued: true
dominant: false
experience: experience/Works
group u list: _42_
m user id: 0
owner id: 6
owning group id: 42
sample status: Discontinued
status: status/Deleted
bookmark: false
star: false
creator: Tom Knight
type: DnaRegion
role: SO:0000155
role: partType/Plasmid
sequence annotation: ccdA- (rev)
sequence annotation: BB-Pre
sequence annotation: terminator
sequence annotation: ccD Operon (rev)
sequence annotation: ORI
sequence annotation: Cm R (rev)
sequence annotation: rep (pMB1) (rev)
sequence annotation: VR
sequence annotation: BB-Post
sequence annotation: VF
sequence annotation: APr (rev)
sequence annotation: VF2
sequence annotation: ccdB (rev)
sequence: https://synbiohub.org/public/igem/pSB1AC3_1_sequence/1

ccdA- (rev)
type: SequenceAnnotation
persistent identity: annotation1569449
display id: annotation1569449
version: 1
top level: pSB1AC3-1
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/pSB1AC3_1/annotation1569449/range1569449/1

https://synbiohub.org/public/igem/pSB1AC3_1/annotation1569449/range1569449/1
type: Range
persistent identity: range1569449
display id: range1569449
version: 1
top level: pSB1AC3-1
direction: direction/forward
start: 359
end: 579
orientation: inline

BB-Pre
type: SequenceAnnotation
persistent identity: annotation1569474
display id: annotation1569474
version: 1
top level: pSB1AC3-1
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/pSB1AC3_1/annotation1569474/range1569474/1

https://synbiohub.org/public/igem/pSB1AC3_1/annotation1569474/range1569474/1
type: Range
persistent identity: range1569474
display id: range1569474
version: 1
top level: pSB1AC3-1
direction: direction/forward
start: 3
end: 18
orientation: inline

terminator
type: SequenceAnnotation
persistent identity: annotation1569481
display id: annotation1569481
version: 1
top level: pSB1AC3-1
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/pSB1AC3_1/annotation1569481/range1569481/1

https://synbiohub.org/public/igem/pSB1AC3_1/annotation1569481/range1569481/1
type: Range
persistent identity: range1569481
display id: range1569481
version: 1
top level: pSB1AC3-1
direction: direction/forward
start: 728
end: 762
orientation: inline

ccD Operon (rev)
type: SequenceAnnotation
persistent identity: annotation1569482
display id: annotation1569482
version: 1
top level: pSB1AC3-1
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB1AC3_1/annotation1569482/range1569482/1

https://synbiohub.org/public/igem/pSB1AC3_1/annotation1569482/range1569482/1
type: Range
persistent identity: range1569482
display id: range1569482
version: 1
top level: pSB1AC3-1
direction: direction/forward
start: 23
end: 699
orientation: inline

ORI
type: SequenceAnnotation
persistent identity: annotation1569490
display id: annotation1569490
version: 1
top level: pSB1AC3-1
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB1AC3_1/annotation1569490/range1569490/1

https://synbiohub.org/public/igem/pSB1AC3_1/annotation1569490/range1569490/1
type: Range
persistent identity: range1569490
display id: range1569490
version: 1
top level: pSB1AC3-1
direction: direction/forward
start: 974
end: 974
orientation: inline

Cm R (rev)
type: SequenceAnnotation
persistent identity: annotation1569434
display id: annotation1569434
version: 1
top level: pSB1AC3-1
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/pSB1AC3_1/annotation1569434/range1569434/1

https://synbiohub.org/public/igem/pSB1AC3_1/annotation1569434/range1569434/1
type: Range
persistent identity: range1569434
display id: range1569434
version: 1
top level: pSB1AC3-1
direction: direction/forward
start: 1861
end: 2519
orientation: inline

rep (pMB1) (rev)
type: SequenceAnnotation
persistent identity: annotation1569479
display id: annotation1569479
version: 1
top level: pSB1AC3-1
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB1AC3_1/annotation1569479/range1569479/1

https://synbiohub.org/public/igem/pSB1AC3_1/annotation1569479/range1569479/1
type: Range
persistent identity: range1569479
display id: range1569479
version: 1
top level: pSB1AC3-1
direction: direction/forward
start: 959
end: 1573
orientation: inline

VR
type: SequenceAnnotation
persistent identity: annotation1569485
display id: annotation1569485
version: 1
top level: pSB1AC3-1
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/pSB1AC3_1/annotation1569485/range1569485/1

https://synbiohub.org/public/igem/pSB1AC3_1/annotation1569485/range1569485/1
type: Range
persistent identity: range1569485
display id: range1569485
version: 1
top level: pSB1AC3-1
direction: direction/forward
start: 855
end: 874
orientation: inline

BB-Post
type: SequenceAnnotation
persistent identity: annotation1569478
display id: annotation1569478
version: 1
top level: pSB1AC3-1
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/pSB1AC3_1/annotation1569478/range1569478/1

https://synbiohub.org/public/igem/pSB1AC3_1/annotation1569478/range1569478/1
type: Range
persistent identity: range1569478
display id: range1569478
version: 1
top level: pSB1AC3-1
direction: direction/forward
start: 701
end: 719
orientation: inline

VF
type: SequenceAnnotation
persistent identity: annotation1569484
display id: annotation1569484
version: 1
top level: pSB1AC3-1
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/pSB1AC3_1/annotation1569484/range1569484/1

https://synbiohub.org/public/igem/pSB1AC3_1/annotation1569484/range1569484/1
type: Range
persistent identity: range1569484
display id: range1569484
version: 1
top level: pSB1AC3-1
direction: direction/forward
start: 3534
end: 3553
orientation: inline

terminator
type: SequenceAnnotation
persistent identity: annotation1569480
display id: annotation1569480
version: 1
top level: pSB1AC3-1
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/pSB1AC3_1/annotation1569480/range1569480/1

https://synbiohub.org/public/igem/pSB1AC3_1/annotation1569480/range1569480/1
type: Range
persistent identity: range1569480
display id: range1569480
version: 1
top level: pSB1AC3-1
direction: direction/forward
start: 3703
end: 3722
orientation: inline

APr (rev)
type: SequenceAnnotation
persistent identity: annotation1569438
display id: annotation1569438
version: 1
top level: pSB1AC3-1
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/pSB1AC3_1/annotation1569438/range1569438/1

https://synbiohub.org/public/igem/pSB1AC3_1/annotation1569438/range1569438/1
type: Range
persistent identity: range1569438
display id: range1569438
version: 1
top level: pSB1AC3-1
direction: direction/forward
start: 2631
end: 3491
orientation: inline

VF2
type: SequenceAnnotation
persistent identity: annotation1780817
display id: annotation1780817
version: 1
top level: pSB1AC3-1
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/pSB1AC3_1/annotation1780817/range1780817/1

https://synbiohub.org/public/igem/pSB1AC3_1/annotation1780817/range1780817/1
type: Range
persistent identity: range1780817
display id: range1780817
version: 1
top level: pSB1AC3-1
direction: direction/forward
start: 3614
end: 3633
orientation: inline

ccdB (rev)
type: SequenceAnnotation
persistent identity: annotation1569439
display id: annotation1569439
version: 1
top level: pSB1AC3-1
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/pSB1AC3_1/annotation1569439/range1569439/1

https://synbiohub.org/public/igem/pSB1AC3_1/annotation1569439/range1569439/1
type: Range
persistent identity: range1569439
display id: range1569439
version: 1
top level: pSB1AC3-1
direction: direction/forward
start: 52
end: 357
orientation: inline

BBa_J23100
type: ComponentDefinition
persistent identity: BBa J23100
display id: BBa_J23100
version: 1
was generated by: iGEM to SBOL conversion
description: constitutive promoter family member
created: 2006-08-03T11:00:00Z
modified: 2015-08-31T04:08:40Z
mutable provenance: Isolated from library of promoters
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Replace later
discontinued: false
dominant: true
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: true
mutable notes: N/A
star: true
creator: John Anderson
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J23100_sequence/1

p(tetR)
type: ComponentDefinition
persistent identity: BBa R0040
display id: BBa_R0040
version: 1
was generated by: iGEM to SBOL conversion
description: TetR repressible promoter
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: Lutz, R., Bujard, H., Nucleic Acids Research (1997) 25, 1203-1210.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Sequence for pTet inverting regulator driven by the TetR protein.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: BBa_R0040 TetR-Regulated Promoter is based on a cI promoter. It has been modified to include two TetR binding sites and the BioBrick standard assembly head and tail restriction sites.
star: true
creator: June Rhee, Connie Tao, Ty Thomson, Louis Waldman
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: BBa_R0040
sequence annotation: TetR 1
sequence annotation: -10
sequence annotation: TetR 2
sequence annotation: -35
sequence: https://synbiohub.org/public/igem/BBa_R0040_sequence/1

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1986784
display id: annotation1986784
version: 1
top level: p(tetR)
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986784/range1986784/1

https://synbiohub.org/public/igem/BBa_R0040/annotation1986784/range1986784/1
type: Range
persistent identity: range1986784
display id: range1986784
version: 1
top level: p(tetR)
direction: direction/forward
start: 1
end: 54
orientation: inline

TetR 1
type: SequenceAnnotation
persistent identity: annotation1986783
display id: annotation1986783
version: 1
top level: p(tetR)
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986783/range1986783/1

https://synbiohub.org/public/igem/BBa_R0040/annotation1986783/range1986783/1
type: Range
persistent identity: range1986783
display id: range1986783
version: 1
top level: p(tetR)
direction: direction/forward
start: 1
end: 19
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1986787
display id: annotation1986787
version: 1
top level: p(tetR)
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986787/range1986787/1

https://synbiohub.org/public/igem/BBa_R0040/annotation1986787/range1986787/1
type: Range
persistent identity: range1986787
display id: range1986787
version: 1
top level: p(tetR)
direction: direction/forward
start: 43
end: 48
orientation: inline

TetR 2
type: SequenceAnnotation
persistent identity: annotation1986786
display id: annotation1986786
version: 1
top level: p(tetR)
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986786/range1986786/1

https://synbiohub.org/public/igem/BBa_R0040/annotation1986786/range1986786/1
type: Range
persistent identity: range1986786
display id: range1986786
version: 1
top level: p(tetR)
direction: direction/forward
start: 26
end: 44
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1986785
display id: annotation1986785
version: 1
top level: p(tetR)
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986785/range1986785/1

https://synbiohub.org/public/igem/BBa_R0040/annotation1986785/range1986785/1
type: Range
persistent identity: range1986785
display id: range1986785
version: 1
top level: p(tetR)
direction: direction/forward
start: 20
end: 25
orientation: inline

https://synbiohub.org/public/igem/BBa_K394001_sequence/1
type: Sequence
persistent identity: BBa K394001 sequence
display id: BBa_K394001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tactagtagcggccgctgcagagagggagaagcagtagcagaacaggccacacaatcgcaagtgattaacgaagacgattacttcgcgttatgcaggcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaaggacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagctcgagattctcatgtttgacagcttatcatcgataagctttaatgcggtagtttatcacagttaaattgctaacgcagtcaggcaccgtgtatgaaatctaacaatgcgctcatcgtcatcctcggcaccgtcaccctggatgctgtaggcataggcttggttatgccggtactgccgggcctcttgcgggatatcgtccattccgacagcatcgccagtcactatggcgtgctgctagcgctatatgcgttgatgcaatttctatgcgcacccgttctcggagcactgtccgaccgctttggccgccgcccagtcctgctcgcttcgctacttggagccactatcgactacgcgatcatggcgaccacacccgtcctgtggatcctctacgccggacgcatcgtggccggcatcaccggcgccacaggtgcggttgctggcgcctatatcgccgacatcaccgatggggaagatcgggctcgccacttcgggctcatgagcgcttgtttcggcgtgggtatggtggcaggccccgtggccgggggactgttgggcgccatctccttgcatgcaccattccttgcggcggcggtgctcaacggcctcaacctactactgggctgcttcctaatgcaggagtcgcataagggagagcgtcgaccgatgcccttgagagccttcaacccagtcagctccttccggtgggcgcggggcatgactatcgtcgccgcacttatgactgtcttctttatcatgcaactcgtaggacaggtgccggcagcgctctgggtcattttcggcgaggaccgctttcgctggagcgcgacgatgatcggcctgtcgcttgcggtattcggaatcttgcacgccctcgctcaagccttcgtcactggtcccgccaccaaacgtttcggcgagaagcaggccattatcgccggcatggcggccgacgcgctgggctacgtct…
encoding: naseq.html

https://synbiohub.org/public/igem/pSB1AC3_1_sequence/1
type: Sequence
persistent identity: pSB1AC3 1 sequence
display id: pSB1AC3_1_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ggaattcgcggccgcatctagagactggctgtgtataagggagcctgacatttatattccccagaacatcaggttaatggcgtttttgatgtcattttcgcggtggctgagatcagccacttcttccccgataacggagaccggcacactggccatatcggtggtcatcatgcgccagctttcatccccgatatgcaccaccgggtaaagttcacgggagactttatctgacagcagacgtgcactggccagggggatcaccatccgtcgcccgggcgtgtcaataatatcactctgtacatccacaaacagacgataacggctctctcttttataggtgtaaaccttaaactgcatttcaccagcccctgttctcgtcagcaaaagagccgttcatttcaataaaccgggcgacctcagccatcccttcctgattttccgctttccagcgttcggcacgcagacgacgggcttcattctgcatggttgtgcttaccagaccggagatattgacatcatatatgccttgagcaactgatagctgtcgctgtcaactgtcactgtaatacgctgcttcatagcatacctctttttgacatacttcgggtatacatatcagtatatattcttataccgcaaaaatcagcgcgcaaatacgcatactgttatctggcttttagtaagccggatccacgcgttactagtagcggccgctgcagtccggcaaaaaaacgggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaagattacttcgcgttatgcaggcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaaggacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagctcgaggcttggattctcaccaataaaaaacgcccggcggcaaccgagcgttctgaacaaatccagatggagttctgaggtcattactggatctatcaacaggagtccaagcgagctcgatatcaaattacgccccgccctgccactcatcgcagtactgttgtaattcattaagcattctgccgacatggaagccatcacaaacggcatgatgaacctgaatcgccagcggcatcagcaccttgtcgccttgcgtataatatttgcc…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I11007_sequence/1
type: Sequence
persistent identity: BBa I11007 sequence
display id: BBa_I11007_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ctcgagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcacatcagcaggacgcactgaccgaattcattaaagaggagaaaggtaccatgagaggatccgtcgacctgcagccaagcttaattagctgatctagaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctcctgagtaggacaaatccgccgccctagacctaggcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcaatgctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaaggacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgactagtgcttggattctcaccaataaaaaacgcccggcggcaaccgagcgttctgaacaaatccagatggagttctgaggtcattactggatctatcaacaggagtccaagcgagctctcgaaccccagagtcccgctcagaagaactcgtcaagaaggcgatagaaggcgatgcgctgcgaatcgggagcggcgataccgtaaagcacgaggaagcggtcagcccattcgccgccaagctcttcagcaatatcacgggtagccaacgctatgtcctgatagcggtccgccacacccagccggccacagtcgatgaatccagaaaagcggccattttccaccatgatattcggcaagcaggcatcgccatgggtcacgacgagatcctcgccgtcgggcatgcgcgccttgagcctggcgaacagttcggctggcgcgagcccctgatgctcttcgtccagatcatcctgatcgacaagaccggcttccatccgagtacgtgctcgctcgatgcgatgtttcgcttggtggtcgaatgggcaggtagccggatcaagcgtatgcagccgccgcattgcatcagccatgatggatactttctcggcaggagcaaggtgagatgacaggagatcctgccccggcacttcgcccaatagcagccagtcccttcccgcttcagtgacaacgtcgagcacagctgcgcaaggaacgcccgtcgtggccagccacgatagccgcgctgcctcgtcctgcagttcattcagggcaccggacaggtcggtcttgacaaaaagaaccgggcgcccctgcgctgacagccggaacacggcggcatcagagcagccgattgtctgttgtgcccagtcatagccgaatagcctctccacccaagcggccggagaacctgcgtgcaatccatcttgttc…
encoding: naseq.html

https://synbiohub.org/public/igem/pSB1A10_sequence/1
type: Sequence
persistent identity: pSB1A10 sequence
display id: pSB1A10_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tactagtagcggccgctgcagcgatccattgcagtggtattatttgtattgatctccttactatctctcgagagattagtacctttggagatctactagagaaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtcaattgaggtagaggtacacacgcgaactccgatagccaattcagagtaataaactgtgataatcaatgcagtccggcaaaaaaacgggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaagattacttcgcgttatgcaggcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaaggacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaat…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0071_sequence/1
type: Sequence
persistent identity: BBa C0071 sequence
display id: BBa_C0071_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaggaatgacggaggctttttgctgtggtgggacggtttgcgtagcgagatgcagccgatccacgacagccagggcgtgttcgccgtcctggaaaaggaagtgcggcgcctgggcttcgattactacgcctatggcgtgcgccacacgattcccttcacccggccgaagaccgaggtccatggcacctatcccaaggcctggctggagcgataccagatgcagaactacggggccgtggatccggcgatcctcaacggcctgcgctcctcggaaatggtggtctggagcgacagcctgttcgaccagagccggatgctctggaacgaggctcgcgattggggcctctgtgtcggcgcgaccttgccgatccgcgcgccgaacaatttgctcagcgtgctttccgtggcgcgcgaccagcagaacatctccagcttcgagcgcgaggaaatccgcctgcggctgcgttgcatgatcgagttgctgacccagaagctgaccgacctggagcatccgatgctgatgtccaacccggtctgcctgagccatcgcgaacgcgagatcctgcaatggaccgccgacggcaagagttccggggaaatcgccatcatcctgagcatctccgagagcacggtgaacttccaccacaagaacatccagaagaagttcgacgcgccgaacaagacgctggctgccgcctacgccgcggcgctgggtctcatcgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J23100_sequence/1
type: Sequence
persistent identity: BBa J23100 sequence
display id: BBa_J23100_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgacggctagctcagtcctaggtacagtgctagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1976000_sequence/1
type: Sequence
persistent identity: BBa K1976000 sequence
display id: BBa_K1976000_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaaaaaaaaccccgcttcggcggggttttttttttacaggtcactaataccatctaagtagttgattcatagtgactggatatgttgtgttttacagtattatgtagtctgttttttatgcaaaatctaatttaatatattgatatttatatcattttacgtttctcgttcagcttttttatactaagttggcattataagaaagcattgcttatcaatttgttgcaacgaacaggtcactatcagtcaaaataaaatcattatttaaaaaaaaaccccgcttcggcggggttttttttttactagagtttacagctagctcagtcctaggtattatgctagctactagagtcacacaggaaagtactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaagcagcaaacgacgaaaactacgctttagtagcttaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K394002_sequence/1
type: Sequence
persistent identity: BBa K394002 sequence
display id: BBa_K394002_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tactagtagcggccgctgcagtcttaacccaactgcacagaacaataacctgctggaaacgaagataaatcgaagacgattacttcgcgttatgcaggcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaaggacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagctcgagattctcatgtttgacagcttatcatcgataagctttaatgcggtagtttatcacagttaaattgctaacgcagtcaggcaccgtgtatgaaatctaacaatgcgctcatcgtcatcctcggcaccgtcaccctggatgctgtaggcataggcttggttatgccggtactgccgggcctcttgcgggatatcgtccattccgacagcatcgccagtcactatggcgtgctgctagcgctatatgcgttgatgcaatttctatgcgcacccgttctcggagcactgtccgaccgctttggccgccgcccagtcctgctcgcttcgctacttggagccactatcgactacgcgatcatggcgaccacacccgtcctgtggatcctctacgccggacgcatcgtggccggcatcaccggcgccacaggtgcggttgctggcgcctatatcgccgacatcaccgatggggaagatcgggctcgccacttcgggctcatgagcgcttgtttcggcgtgggtatggtggcaggccccgtggccgggggactgttgggcgccatctccttgcatgcaccattccttgcggcggcggtgctcaacggcctcaacctactactgggctgcttcctaatgcaggagtcgcataagggagagcgtcgaccgatgcccttgagagccttcaacccagtcagctccttccggtgggcgcggggcatgactatcgtcgccgcacttatgactgtcttctttatcatgcaactcgtaggacaggtgccggcagcgctctgggtcattttcggcgaggaccgctttcgctggagcgcgacgatgatcggcctgtcgcttgcggtattcggaatcttgcacgccctcgctcaagccttcgtcactggtcccgccaccaaacgtttcggcgagaagcaggccattatcgccggcatggcggccgacgcgctgggctacgtc…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I11006_sequence/1
type: Sequence
persistent identity: BBa I11006 sequence
display id: BBa_I11006_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ctcgagaattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatcagcaggacgcactgaccgaattcattaaagaggagaaaggtaccatggaagacgccaaaaacataaagaaaggcccggcgccattctatccgctagaggatggaaccgctggagagcaactgcataaggctatgaagagatacgccctggttcctggaacaattgcttttacagatgcacatatcgaggtgaacatcacgtacgcggaatacttcgaaatgtccgttcggttggcagaagctatgaaacgatatgggctgaatacaaatcacagaatcgtcgtatgcagtgaaaactctcttcaattctttatgccggtgttgggcgcgttatttatcggagttgcagttgcgcccgcgaacgacatttataatgaacgtgaattgctcaacagtatgaacatttcgcagcctaccgtagtgtttgtttccaaaaaggggttgcaaaaaattttgaacgtgcaaaaaaaattaccaataatccagaaaattattatcatggattctaaaacggattaccagggatttcagtcgatgtacacgttcgtcacatctcatctacctcccggttttaatgaatacgattttgtaccagagtcctttgatcgtgacaaaacaattgcactgataatgaactcctctggatctactgggttacctaagggtgtggcccttccgcatagaactgcctgcgtcagattctcgcatgccagagatcctatttttggcaatcaaatcattccggatactgcgattttaagtgttgttccattccatcacggttttggaatgtttactacactcggatatttgatatgtggatttcgagtcgtcttaatgtatagatttgaagaagagctgtttttacgatcccttcaggattacaaaattcaaagtgcgttgctagtaccaaccctattttcattcttcgccaaaagcactctgattgacaaatacgatttatctaatttacacgaaattgcttctgggggcgcacctctttcgaaagaagtcggggaagcggttgcaaaacgcttccatcttccagggatacgacaaggatatgggctcactgagactacatcagctattctgattacacccgagggggatgataaaccgggcgcggtcggtaaagttgttccattttttgaagcgaaggttgtggatctggataccgggaaaacgctgggcgttaatcagagaggcgaattatgtgtcagaggacctatgattatgtccggttatgtaaacaatccggaagcgaccaacgccttgattgacaaggatggatggctacattctggagacatagcttactgggacgaagacgaacacttcttcatagttgaccgcttgaagtctttaattaaatacaaaggataccaggtggcccccgctgaattggagtcgatattgttacaacaccccaacatcttcgacgcgggcgtggcaggtcttcccgacgatgacgccggtgaacttcccgccgccgttgttgttttggagcacggaaagacgatgacggaaaaagagatcgtggattacgtcgccagtcaagtaacaaccgccaaaaagttgcgcggaggagttgtgtttgtggacgaagtaccgaaaggtcttaccggaaaactcgacgcaagaaaaatcagagagatcctcataaaggccaagaagggcggaaagtccaaattgtaatgactctagaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctcctgagtaggacaaatccgccgccctagacctaggcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaacc…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K283042_sequence/1
type: Sequence
persistent identity: BBa K283042 sequence
display id: BBa_K283042_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatactagatgaggaatgacggaggctttttgctgtggtgggacggtttgcgtagcgagatgcagccgatccacgacagccagggcgtgttcgccgtcctggaaaaggaagtgcggcgcctgggcttcgattactacgcctatggcgtgcgccacacgattcccttcacccggccgaagaccgaggtccatggcacctatcccaaggcctggctggagcgataccagatgcagaactacggggccgtggatccggcgatcctcaacggcctgcgctcctcggaaatggtggtctggagcgacagcctgttcgaccagagccggatgctctggaacgaggctcgcgattggggcctctgtgtcggcgcgaccttgccgatccgcgcgccgaacaatttgctcagcgtgctttccgtggcgcgcgaccagcagaacatctccagcttcgagcgcgaggaaatccgcctgcggctgcgttgcatgatcgagttgctgacccagaagctgaccgacctggagcatccgatgctgatgtccaacccggtctgcctgagccatcgcgaacgcgagatcctgcaatggaccgccgacggcaagagttccggggaaatcgccatcatcctgagcatctccgagagcacggtgaacttccaccacaagaacatccagaagaagttcgacgcgccgaacaagacgctggctgccgcctacgccgcggcgctgggtctcatcgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0032_sequence/1
type: Sequence
persistent identity: BBa B0032 sequence
display id: BBa_B0032_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacacaggaaag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K283041_sequence/1
type: Sequence
persistent identity: BBa K283041 sequence
display id: BBa_K283041_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgacggctagctcagtcctaggtacagtgctagctactagagaaagaggagaaatactagatgaggaatgacggaggctttttgctgtggtgggacggtttgcgtagcgagatgcagccgatccacgacagccagggcgtgttcgccgtcctggaaaaggaagtgcggcgcctgggcttcgattactacgcctatggcgtgcgccacacgattcccttcacccggccgaagaccgaggtccatggcacctatcccaaggcctggctggagcgataccagatgcagaactacggggccgtggatccggcgatcctcaacggcctgcgctcctcggaaatggtggtctggagcgacagcctgttcgaccagagccggatgctctggaacgaggctcgcgattggggcctctgtgtcggcgcgaccttgccgatccgcgcgccgaacaatttgctcagcgtgctttccgtggcgcgcgaccagcagaacatctccagcttcgagcgcgaggaaatccgcctgcggctgcgttgcatgatcgagttgctgacccagaagctgaccgacctggagcatccgatgctgatgtccaacccggtctgcctgagccatcgcgaacgcgagatcctgcaatggaccgccgacggcaagagttccggggaaatcgccatcatcctgagcatctccgagagcacggtgaacttccaccacaagaacatccagaagaagttcgacgcgccgaacaagacgctggctgccgcctacgccgcggcgctgggtctcatcgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1937005_sequence/1
type: Sequence
persistent identity: BBa K1937005 sequence
display id: BBa_K1937005_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tctaattcttgatcgtttgttacaatcatctgtcattcccgctttctttttaaataatgtagattaagcttaccacaactgtcttaaaaataggaaacacacggacctgggaaaaaagaaatacccccgggaaaattggtatagatcactagatatcttatatggtatatttgaaaaaaaagggtatgagggggatgggtgctagcatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgcccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K319043_sequence/1
type: Sequence
persistent identity: BBa K319043 sequence
display id: BBa_K319043_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gaattgaacgctcgtaagtaaatattgatttatacatgtataagtttttcaagatttaaggaaaaaagaaaagttattatctttctccgcggtttttcaatgacccgatgtattgactcttcctgaccgaaaataacaggacagagttagaacgaacatgaataataaaatataatctgggatcatgaatccgaatgactgctcatagtaacaaccaggcagaagcgaattcgggggcccccgggggctgcagggtgactggcctttgtctgaatagattgttccgaacttgttcagttgtatgtaaaatagtttccaaatgcgcccatttatacataattatctttgtattctgacctaaactaaatatatatatatatatgcacgtattaaaaaaaaaaataaaagcaaaaaggtaaacaaagagaagtaatccgcaacttgtacaaaaagaaaagaagataaaagaattgatgcagtccggcaaaaaaacgggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaagattacttcgcgttatgcaggcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaaggacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagctcgaggcttggattctcaccaataaaaaacgcccggcggcaaccgagcgttctgaacaaatccagatggagttctgaggtcattactggatctatcaacaggagtccaagcgagctcgatatcaaattacgccccgccctgccactcatcgcagtactgttgtaattcattaagcattctgccgacatggaagccatcacaaacggcatgatgaacctgaatcgccagcggcatcagcaccttgtcgccttgcgtataatatttgcccatggtgaaaacgggggcgaagaagttgtccatattggccacgtttaaatcaaaactggtgaaactcacccagggattggctgagacgaaaaacatattctcaataaaccctttagggaaataggccaggttttcaccgtaacacgccacatcttgcgaatatatgtgtagaaactgccggaaatcgtcgtggtattcactccagagcgatgaaaacgtttcagtttgctcatggaaaac…
encoding: naseq.html

https://synbiohub.org/public/igem/pSB1A1_sequence/1
type: Sequence
persistent identity: pSB1A1 sequence
display id: pSB1A1_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tactagtagcggccgctgcagggatcctctagagtcgacctgcaggcatgcaagcttggcgtaatcatggtcatagctgtttcctgtgtgaaattgttatccgctcacaattccacacaacatacgagccggaagcataaagtgtaaagcctggggtgcctaatgagtgagctaactcacattaattgcgttgcgctcactgcccgctttccagtcgggaaacctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaaggacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagttaccaatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgccattgctacaggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagctccggttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggttatggcagcactgcataattctcttactgtcatgccatccgtaagatgcttttctgtgactggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcgccacatagcagaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatcttaccgctgttgagatccagttcgatgtaacccactcgtgcacccaactgatcttcagcatctttta…
encoding: naseq.html

https://synbiohub.org/public/igem/pSB2K0_sequence/1
type: Sequence
persistent identity: pSB2K0 sequence
display id: pSB2K0_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgagccatattcaacgggaaacgtcttgctcgaggccgcgattaaattccaacatggatgctgatttatatgggtataaatgggctcgcgataatgtcgggcaatcaggtgcgacaatctatcgattgtatgggaagcccgatgcgccagagttgtttctgaaacatggcaaaggtagcgttgccaatgatgttacagatgagatggtcagactaaactggctgacggaatttatgcctcttccgaccatcaagcattttatccgtactcctgatgatgcatggttactcaccactgcgatccccgggaaaacagcattccaggtattagaagaatatcctgattcaggtgaaaatattgttgatgcgctggcagtgttcctgcgccggttgcattcgattcctgtttgtaattgtccttttaacagcgatcgcgtatttcgtctcgctcaggcgcaatcacgaatgaataacggtttggttgatgcgagtgattttgatgacgagcgtaatggctggcctgttgaacaagtctggaaagaaatgcataaactgttgccattctcaccggattcagtcgtcactcatggtgatttctcacttgataaccttatttttgacgaggggaaattaataggttgtattgatgttggacgagtcggaatcgcagaccgataccaggatcttgccatcctatggaactgcctcggtgagttttctccttcattacagaaacggctttttcaaaaatatggtattgataatcctgatatgaataaattgcagtttcatttgatgctcgatgagtttttctaatcagaattggttaattggttgtaacactggcgagctcggatcgcggccgtcgctaccttaggaccgttatagtagggataacagggtaatggcgccgacgtcggccgaggcccgggcgtttaaacatttaaatgtcgacatacgatttaggtgacactatagaactctaatacgactcactatagggaatttggccctcgaggccaagaattcccgactacgtagtcggggatccactagtgcatgcatatgcggccgcaagcttaattaacctggtagcttattccctttagtgagggttaattttagcttggcactggccgtcgttttacaacgtcgtgactgggaaaaccctgttaaccggcgcgccacgcgtacccataatacccataatagctgtttgccatcgcgtatgcatcgatcacgtgtccacgttctttaatagtggactcttgttccaaactggaacaacactcggatcgatccggcgcgcaccgtgggaaaaactccaggtagaggtacacacgcggatagccaattcagagtaataaactgtgataatcaaccctcatcaatgatgacgaactaacccccgatatcaggtcacatgacgaagggaaagagaaggaaatcaactgtgacaaactgccctcaaatttggcttccttaaaaattacagttcaaaaagtatgagaaaatccatgcaggctgaaggaaacagcaaaactgtgacaaattaccctcagtaggtcagaacaaatgtgacgaaccaccctcaaatctgtgacagataaccctcagactatcctgtcgtcatggaagtgatatcgcggaaggaaaatacgatatgagtcgtctggcggcctttctttttctcaatgtatgagaggcgcattggagttctgctgttgatctcattaacacagacctgcaggaagcggcggcggaagtcaggcatacgctggtaactttgaggcagctggtaacgctctatgatccagtcgattttcagagagacgatgcctgagccatccggcttacgatactgacacagggattcgtataaacgcatggcatacggattggtgatttcttttgtttcactaagccgaaactgcgtaaaccggttctgtaacccgataaagaagggaatgagatatgggttgatatgtacactgtaaagc…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1976004_sequence/1
type: Sequence
persistent identity: BBa K1976004 sequence
display id: BBa_K1976004_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaaaaaaaaccccgcttcggcggggttttttttttacaggtcactaataccatctaagtagttgattcatagtgactggatatgttgtgttttacagtattatgtagtctgttttttatgcaaaatctaatttaatatattgatatttatatcattttacgtttctcgttcagcttttttatactaagttggcattataagaaagcattgcttatcaatttgttgcaacgaacaggtcactatcagtcaaaataaaatcattatttaaaaaaaaaccccgcttcggcggggttttttttt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1976001_sequence/1
type: Sequence
persistent identity: BBa K1976001 sequence
display id: BBa_K1976001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tttacagctagctcagtcctaggtattatgctagctactagaaagaggagaaaatgggaagaaggcgaagtcatgagcgccgggatttaccccctaacctttatataagaaacaatggatattactgctacagggacccaaggacgggtaaagagtttggattaggcagagacaggcgaatcgcaatcactgaagctatacaggccaacattgagttattttcaggacacaaacacaagcctctgacagcgagaatcaacagtgataattccgttacgttacattcatggcttgatcgctacgaaaaaatcctggccagcagaggaatcaagcagaagacactcataaattacatgagcaaaattaaagcaataaggaggggtctgcctgatgctccacttgaagacatcaccacaaaagaaattgcggcaatgctcaatggatacatagacgagggcaaggcggcgtcagccaagttaatcagatcaacactgagcgatgcattccgagaggcaatagctgaaggccatataacaacaaaccatgtcgctgccactcgcgcagcaaaatcagaggtaaggagatcaagacttacggctgacgaatacctgaaaatttatcaagcagcagaatcatcaccatgttggctcagacttgcaatggaactggctgttgttaccgggcaacgagttggtgatttatgcgaaatgaagtggtctgatatcgtagatggatatctttatgtcgagcaaagcaaaacaggcgtaaaaattgccatcccaacagcattgcatattgatgctctcggaatatcaatgaaggaaacacttgataaatgcaaagagattcttggcggagaaaccataattgcatctactcgtcgcgaaccgctttcatccggcacagtatcaaggtattttatgcgcgcacgaaaagcatcaggtctttccttcgaaggggacccgcctacctttcacgagttgcgcagtttgtctgcaagactctatgagaagcagataagcgataagtttgctcaacatcttctcgggcataagtcggacaccatggcatcacagtatcgtgatgacagaggcagggagtgggacaaaattgaaatcaaataataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I11008_sequence/1
type: Sequence
persistent identity: BBa I11008 sequence
display id: BBa_I11008_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gacgtctgtgtggaattgtgagcggataacaatttcacacagggccctcggacaccgaggagaatgtcaagaggcgaacacacaacgtcttggagcgccagaggaggaacgagctaaaacggagcttttttgccctgcgtgaccagatcccggagttggaaaacaatgaaaaggcccccaaggtagttatccttaaaaaagccacagcatacatcctgtccgtccaagcagaggagcaaaagctcatttctgaagaggacttgttgcggaaacgacgagaacagttgaaacacaaacttgaacagctacggaactcttgtgcgtaaggaaaagtaaggaaaacgattccttctaacagaaatgtcctgagcaatcacctatgaactgtcgactcgagcatagcatttttatccataagattagcggatcctaagctttacaattgtgagcgctcacaattatgatagattcaattgtgagcggataacaatttcacacagaattcattaaagaggagaaaggtaccgggccccccctcgaggtcgacggtatcgataagcttgatatcgaattcctgcagcccgggggatcccatggtacgcgtgctagaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctcctgagtaggacaaatccgccgccctagacctagggtacgggttttgctgcccgcaaacgggctgttctggtgttgctagtttgttatcagaatcgcagatccggcttcaggtttgccggctgaaagcgctatttcttccagaattgccatgattttttccccacgggaggcgtcactggctcccgtgttgtcggcagctttgattcgataagcagcatcgcctgtttcaggctgtctatgtgtgactgttgagctgtaacaagttgtctcaggtgttcaatttcatgttctagttgctttgttttactggtttcacctgttctattaggtgttacatgctgttcatctgttacattgtcgatctgttcatggtgaacagctttaaatgcaccaaaaactcgtaaaagctctgatgtatctatcttttttacaccgttttcatctgtgcatatggacagttttccctttgatatctaacggtgaacagttgttctacttttgtttgttagtcttgatgcttcactgatagatacaagagccataagaacctcagatccttccgtatttagccagtatgttctctagtgtggttcgttgtttttgcgtgagccatgagaacgaaccattgagatcatgcttactttgcatgtcactcaaaaattttgcctcaaaactggtgagctgaatttttgcagttaaagcatcgtgtagtgtttttcttagtccgttacgtaggtaggaatctgatgtaatggttgttggtattttgtcaccattcatttttatctggttgttctcaagttcggttacgagatccatttgtctatctagttcaacttggaaaatcaacgtatcagtcgggcggcctcgcttatcaaccaccaatttcatattgctgtaagtgtttaaatctttacttattggtttcaaaacccattggttaagccttttaaactcatggtagttattttcaagcattaacatgaacttaaattcatcaaggctaatctctatatttgccttgtgagttttcttttgtgttagttcttttaataaccactcataaatcctcatagagtatttgttttcaaaagacttaacatgttccagattatattttatgaatttttttaactggaaaagataaggcaatatctcttcactaaaaactaattctaatttttcgcttgagaacttggcatagtttgtccactggaaaatctcaaagcctttaaccaaaggattcctgatttccacagttctcgtcatcagctctctggttgctttagctaatacaccataagcattttccc…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I11003_sequence/1
type: Sequence
persistent identity: BBa I11003 sequence
display id: BBa_I11003_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gacgtctaagaaaccattattatcatgacattaacctataaaaataggcgtatcacgaggccctttcgtcttcacctcgacaattcgcgcgcgaaggcgaagcggcatgcatttacgttgacaccatcgaatggtgcaaaacctttcgcggtatggcatgatagcgcccggaagagagtcaattcagggtggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtaagttagcgcgaattgtcgagggaaatcataaaaaatttatttgctttcaggaaaatttttctgtataatagattcataaatttgagagaagagtttaaatatggctggttctcgcagaaagaaacatatccatgaaatcccgccccgaattcatatgtctagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataaggctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcag…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1976005_sequence/1
type: Sequence
persistent identity: BBa K1976005 sequence
display id: BBa_K1976005_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaagcagcaaacgacgaaaactacgctttagtagcttaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I50000_sequence/1
type: Sequence
persistent identity: BBa I50000 sequence
display id: BBa_I50000_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcgagtgagcgaggaagcaccagggaacagcacttatatattctgcttacacacgatgcctgaaaaaacttcccttggggttatccacttatccacggggatatttttataattattttttttatagtttttagatcttcttttttagagcgccttgtaggcctttatccatgctggttctacagaaggtgttgtgacaaattgccctttcagtgtgacaaatcaccctcaaatgacagtcctgtctgtgacaaattgcccttaaccctgtgacaaattgccctcagaagaagctgttttttcacaaagttatccctgcttattgactcttttttatttagtgtgacaatctaaaaacttgtcacacttcacatggatctgtcatggcggaaacagcggttatcaatcacaagaaacgtaaaaatagcccgcgaatcgtccagtcaaacgacctcactgaggcggcatatagtctctcccgggatcaaaaacgtatgctgtatctgttcgttgaccagatcagaaaatctgatggcaccctacaggaacatgacggtatctgcgagatccatgttgctaaatatgctgaaatattcggattgacctctgcggaagccagtaaggatatacggcaggcattgaagagtttcgcggggaaggaagtggttttttatcgccctgaagaggatgccggcgatgaaaaaggctatgaatcttttccttggtttatcaaacgtgcgcacagtccatccagagggctttacagtgtacatatcaacccatatctcattcccttctttatcgggttacagaaccggtttacgcagtttcggcttagtgaaacaaaagaaatcaccaatccgtatgccatgcgtttatacgaatccctgtgtcagtatcgtaagccggatggctcaggcatcgtctctctgaaaatcgactggatcatagagcgttaccagctgcctcaaagttaccagcgtatgcctgacttccgccgccgcttcctgcaagtctgtgttaatgagatcaacagcagaactccaatgcgcctctcatacattgagaaaaagaaaggccgccagacgactcatatcgtattttccttccgcgatatcacttccatgacgacaggatagtctgagggttatctgtcacagatttgagggtggttcgtcacatttgttctgacctactgagggtaatttgtcacagttttgctgtttccttcagcctgcatggattttctcatactttttgaactgtaatttttaaggaagccaaatttgagggcagtttgtcacagttgatttccttctctttcccttcgtcatgtgacttgatatcgggggttagttcgtcatcattgatgagggttgattatcacagtttattactctgaattggctatccgcgtgtgtacctctacctggagtttttcccacggtggatatttcttcttgcgctgagcgtaagagctatctgacagaacagttcttctttgcttcctcgccagttcgctcgctatgctcggttacacggctgcggcgagcgctagtgataataagtgactgaggtatgtgctcttcttatctccttttgtagtgttgctcttattttaaacaactttgcggttttttgatgactttgcgattttgttgttgctttgcagtaaattgcaagatttaataaaaaaacgcaaagcaatgattaaaggatgttcagaatgaaactcatggaaacacttaaccagtgcataaacgctggtcatgaaatgacgaaggctatcgccattgcacagtttaatgatgacagcccggaagcgaggaaaataacccggcgctggagaataggtgaagcagcggatttagttggggtttcttctcaggctatcagagatgccgagaaagcagggcgactaccgcacccggatatggaaattcgaggacgggttgagcaacgtgttggttatacaattgaacaaattaatcatatgcgtg…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0040_sequence/1
type: Sequence
persistent identity: BBa R0040 sequence
display id: BBa_R0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I50010_sequence/1
type: Sequence
persistent identity: BBa I50010 sequence
display id: BBa_I50010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gggttcgagaagggggggcaccccccttcggcgtgcgcggtcacgcgcacagggcgcagccctggttaaaaacaaggtttataaatattggtttaaaagcaggttaaaagacaggttagcggtggccgaaaaacgggcggaaacccttgcaaatgctggattttctgcctgtggacagcccctcaaatgtcaataggtgcgcccctcatctgtcagcactctgcccctcaagtgtcaaggatcgcgcccctcatctgtcagtagtcgcgcccctcaagtgtcaataccgcagggcacttatccccaggcttgtccacatcatctgtgggaaactcgcgtaaaatcaggcgttttcgccgatttgcgaggctggccagctccacgtcgccggccgaaatcgagcctgcccctcatctgtcaacgccgcgccgggtgagtcggcccctcaagtgtcaacgtccgcccctcatctgtcagtgagggccaagttttccgcgaggtatccacaacgccggcggccggccgcggtgtctcgcacacggcttcgacggcgtttctggcgcgtttgcagggccatagacggccgccagcccagcggcgagggcaaccagcccggtgagcgtcgga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1976006_sequence/1
type: Sequence
persistent identity: BBa K1976006 sequence
display id: BBa_K1976006_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacacaggaaagtactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaagcagcaaacgacgaaaactacgctttagtagcttaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/pSB1AT3_1_sequence/1
type: Sequence
persistent identity: pSB1AT3 1 sequence
display id: pSB1AT3_1_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tgccacctgacgtctaagaaaccattattatcatgacattaacctataaaaataggcgtatcacgaggcagaatttcagataaaaaaaatccttagctttcgctaaggatgatttctggaattcgcggccgcatctagagactggctgtgtataagggagcctgacatttatattccccagaacatcaggttaatggcgtttttgatgtcattttcgcggtggctgagatcagccacttcttccccgataacggagaccggcacactggccatatcggtggtcatcatgcgccagctttcatccccgatatgcaccaccgggtaaagttcacgggagactttatctgacagcagacgtgcactggccagggggatcaccatccgtcgcccgggcgtgtcaataatatcactctgtacatccacaaacagacgataacggctctctcttttataggtgtaaaccttaaactgcatttcaccagcccctgttctcgtcagcaaaagagccgttcatttcaataaaccgggcgacctcagccatcccttcctgattttccgctttccagcgttcggcacgcagacgacgggcttcattctgcatggttgtgcttaccagaccggagatattgacatcatatatgccttgagcaactgatagctgtcgctgtcaactgtcactgtaatacgctgcttcatagcatacctctttttgacatacttcgggtatacatatcagtatatattcttataccgcaaaaatcagcgcgcaaatacgcatactgttatctggcttttagtaagccggatccacgcgttactagtagcggccgctgcagtccggcaaaaaaacgggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaagattacttcgcgttatgcaggcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaaggacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagctcgagattctcatgtttgacagcttatcatcgataagctttaatgcggtagtttatcacagttaaattgctaacgcagtcaggcaccgtgtatgaaatctaacaatgcgctcatcgtcatcctcggcaccgtcaccctggatgctgtagg…
encoding: naseq.html

https://synbiohub.org/public/igem/pSB1AK3_1_sequence/1
type: Sequence
persistent identity: pSB1AK3 1 sequence
display id: pSB1AK3_1_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tgccacctgacgtctaagaaaccattattatcatgacattaacctataaaaataggcgtatcacgaggcagaatttcagataaaaaaaatccttagctttcgctaaggatgatttctggaattcgcggccgcatctagagactggctgtgtataagggagcctgacatttatattccccagaacatcaggttaatggcgtttttgatgtcattttcgcggtggctgagatcagccacttcttccccgataacggagaccggcacactggccatatcggtggtcatcatgcgccagctttcatccccgatatgcaccaccgggtaaagttcacgggagactttatctgacagcagacgtgcactggccagggggatcaccatccgtcgcccgggcgtgtcaataatatcactctgtacatccacaaacagacgataacggctctctcttttataggtgtaaaccttaaactgcatttcaccagcccctgttctcgtcagcaaaagagccgttcatttcaataaaccgggcgacctcagccatcccttcctgattttccgctttccagcgttcggcacgcagacgacgggcttcattctgcatggttgtgcttaccagaccggagatattgacatcatatatgccttgagcaactgatagctgtcgctgtcaactgtcactgtaatacgctgcttcatagcatacctctttttgacatacttcgggtatacatatcagtatatattcttataccgcaaaaatcagcgcgcaaatacgcatactgttatctggcttttagtaagccggatccacgcgttactagtagcggccgctgcagtccggcaaaaaaacgggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaagattacttcgcgttatgcaggcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaaggacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagctcgagtcccgtcaagtcagcgtaatgctctgccagtgttacaaccaattaaccaattctgattagaaaaactcatcgagcatcaaatgaaactgcaatttattcatatcaggattatcaataccatatttttgaaaaagccgtttctgta…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I11005_sequence/1
type: Sequence
persistent identity: BBa I11005 sequence
display id: BBa_I11005_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ctcgagaaaatttatcaaaaagagtgttgacttgtgagcggataacaatgatacttagattcaattgtgagcggataacaatttcacacagaattcattaaagaggagaaaggtaccatggaagacgccaaaaacataaagaaaggcccggcgccattctatccgctagaggatggaaccgctggagagcaactgcataaggctatgaagagatacgccctggttcctggaacaattgcttttacagatgcacatatcgaggtgaacatcacgtacgcggaatacttcgaaatgtccgttcggttggcagaagctatgaaacgatatgggctgaatacaaatcacagaatcgtcgtatgcagtgaaaactctcttcaattctttatgccggtgttgggcgcgttatttatcggagttgcagttgcgcccgcgaacgacatttataatgaacgtgaattgctcaacagtatgaacatttcgcagcctaccgtagtgtttgtttccaaaaaggggttgcaaaaaattttgaacgtgcaaaaaaaattaccaataatccagaaaattattatcatggattctaaaacggattaccagggatttcagtcgatgtacacgttcgtcacatctcatctacctcccggttttaatgaatacgattttgtaccagagtcctttgatcgtgacaaaacaattgcactgataatgaactcctctggatctactgggttacctaagggtgtggcccttccgcatagaactgcctgcgtcagattctcgcatgccagagatcctatttttggcaatcaaatcattccggatactgcgattttaagtgttgttccattccatcacggttttggaatgtttactacactcggatatttgatatgtggatttcgagtcgtcttaatgtatagatttgaagaagagctgtttttacgatcccttcaggattacaaaattcaaagtgcgttgctagtaccaaccctattttcattcttcgccaaaagcactctgattgacaaatacgatttatctaatttacacgaaattgcttctgggggcgcacctctttcgaaagaagtcggggaagcggttgcaaaacgcttccatcttccagggatacgacaaggatatgggctcactgagactacatcagctattctgattacacccgagggggatgataaaccgggcgcggtcggtaaagttgttccattttttgaagcgaaggttgtggatctggataccgggaaaacgctgggcgttaatcagagaggcgaattatgtgtcagaggacctatgattatgtccggttatgtaaacaatccggaagcgaccaacgccttgattgacaaggatggatggctacattctggagacatagcttactgggacgaagacgaacacttcttcatagttgaccgcttgaagtctttaattaaatacaaaggataccaggtggcccccgctgaattggagtcgatattgttacaacaccccaacatcttcgacgcgggcgtggcaggtcttcccgacgatgacgccggtgaacttcccgccgccgttgttgttttggagcacggaaagacgatgacggaaaaagagatcgtggattacgtcgccagtcaagtaacaaccgccaaaaagttgcgcggaggagttgtgtttgtggacgaagtaccgaaaggtcttaccggaaaactcgacgcaagaaaaatcagagagatcctcataaaggccaagaagggcggaaagtccaaattgtaatgactctagaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctcctgagtaggacaaatccgccgccctagacctaggcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaag…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J23101_sequence/1
type: Sequence
persistent identity: BBa J23101 sequence
display id: BBa_J23101_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tttacagctagctcagtcctaggtattatgctagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I11004_sequence/1
type: Sequence
persistent identity: BBa I11004 sequence
display id: BBa_I11004_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ctcgagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcacatcagcaggacgcactgaccgaattcattaaagaggagaaaggtaccatggaagacgccaaaaacataaagaaaggcccggcgccattctatccgctagaggatggaaccgctggagagcaactgcataaggctatgaagagatacgccctggttcctggaacaattgcttttacagatgcacatatcgaggtgaacatcacgtacgcggaatacttcgaaatgtccgttcggttggcagaagctatgaaacgatatgggctgaatacaaatcacagaatcgtcgtatgcagtgaaaactctcttcaattctttatgccggtgttgggcgcgttatttatcggagttgcagttgcgcccgcgaacgacatttataatgaacgtgaattgctcaacagtatgaacatttcgcagcctaccgtagtgtttgtttccaaaaaggggttgcaaaaaattttgaacgtgcaaaaaaaattaccaataatccagaaaattattatcatggattctaaaacggattaccagggatttcagtcgatgtacacgttcgtcacatctcatctacctcccggttttaatgaatacgattttgtaccagagtcctttgatcgtgacaaaacaattgcactgataatgaactcctctggatctactgggttacctaagggtgtggcccttccgcatagaactgcctgcgtcagattctcgcatgccagagatcctatttttggcaatcaaatcattccggatactgcgattttaagtgttgttccattccatcacggttttggaatgtttactacactcggatatttgatatgtggatttcgagtcgtcttaatgtatagatttgaagaagagctgtttttacgatcccttcaggattacaaaattcaaagtgcgttgctagtaccaaccctattttcattcttcgccaaaagcactctgattgacaaatacgatttatctaatttacacgaaattgcttctgggggcgcacctctttcgaaagaagtcggggaagcggttgcaaaacgcttccatcttccagggatacgacaaggatatgggctcactgagactacatcagctattctgattacacccgagggggatgataaaccgggcgcggtcggtaaagttgttccattttttgaagcgaaggttgtggatctggataccgggaaaacgctgggcgttaatcagagaggcgaattatgtgtcagaggacctatgattatgtccggttatgtaaacaatccggaagcgaccaacgccttgattgacaaggatggatggctacattctggagacatagcttactgggacgaagacgaacacttcttcatagttgaccgcttgaagtctttaattaaatacaaggataccaggtggcccccgctgaattggagtcgatattgttacaacaccccaacatcttcgacgcgggcgtggcaggtcttcccgacgatgacgccggtgaacttcccgccgccgttgttgttttggagcacggaaagacgatgacggaaaaagagatcgtggattacgtcgccagtcaagtaacaaccgccaaaaagttgcgcggaggagttgtgtttgtggacgaagtaccgaaaggtcttaccggaaaactcgacgcaagaaaaatcagagagatcctcataaaggccaagaagggcggaaagtccaaattgtaatgactctagaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctcctgagtaggacaaatccgccgccctagacctaggggatatattccgcttcctcgctcactgactcgctacgctcggtcgttcgactgcggcgagcggaaatggcttacgaacggggcggagatttcctggaagatgccaggaagatacttaaca…
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z