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was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Booperator test: Inverter NOR biasing in polycistronic design
creator: Srini Devadas, David Gray, Ronny Krashinsky, Debra Lin, and Chris Zheng Liu
created: 2004-01-29T12:00:00Z
modified: 2015-08-31T04:08:11Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is the fourth test chassis for the Booperator and is particular to the polycistronic design. It tests the biasing of the Inverter NOR pair.
owned by: james
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group u list: _1_
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created: 2004-05-22T11:00:00Z
modified: 2015-08-31T04:07:38Z
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dominant: false
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modified: 2015-08-31T04:08:29Z
discontinued: false
dominant: false
experience: experience/None
group u list: _37_
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description: AiiA (-LVA) Tri-part (B0034.C0160.B0015)
creator: robertb
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modified: 2015-08-31T04:07:37Z
discontinued: false
dominant: true
experience: experience/None
group u list: _6_
m user id: 0
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sample status: It's complicated
status: status/Planning
bookmark: false
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modified: 2015-08-31T04:07:30Z
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dominant: false
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bookmark: false
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description: Constitutive Promoter I Test (R0074.E0422)
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created: 2004-01-28T12:00:00Z
modified: 2015-08-31T04:07:39Z
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group u list: _1_
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mutable description: tests the strength of R0074, indicated by strength of expression of reporter
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description: Synchronator design 2, Output-
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modified: 2015-08-31T04:07:41Z
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m user id: 0
owner id: 60
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: R0065.Q04400.Q04121.Q04510.I0461.R0063.I0462.R0011.I0460
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star: false
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component: BBa_Q04510
component: BBa_Q04121
component: BBa_I0462
component: BBa_I0460
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sequence annotation: BBa_F1610
sequence annotation: BBa_I0462
sequence annotation: BBa_Q04400
sequence annotation: BBa_Q04510
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sequence annotation: BBa_R0011
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definition: https://synbiohub.org/public/igem/BBa_Q04510/1

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persistent identity: BBa I13930
display id: BBa_I13930
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: c1 Delay Oscillator Intermediate Construct (I13202.C0051.B0015)
creator: robertb
created: 2004-08-03T11:00:00Z
modified: 2015-08-31T04:07:37Z
discontinued: false
dominant: false
experience: experience/None
group u list: _6_
m user id: 0
owner id: 159
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Natural Lux Cassette + c1 + Term. This construct will be put together with the synthetic c1 promoter being synthesized by Caltech and the various AiiA Generation Constructs to create a variety of c1 Delay Oscillators.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
component: BBa_C0051
component: BBa_I13202
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access: public
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type: SequenceAnnotation
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type: ComponentDefinition
persistent identity: BBa I8035
display id: BBa_I8035
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Booperator aiia test 6: Effect of tetR on production of LuxR
creator: Srini Devadas, David Gray, Ronny Krashinsky, Debra Lin, and Chris Zheng Liu
created: 2004-01-31T12:00:00Z
modified: 2015-08-31T04:08:11Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
component: BBa_B0012
component: BBa_R0062
component: BBa_E0032
component: BBa_B0034
component: BBa_B0010
component: BBa_R0040
component: BBa_C0062
sequence annotation: BBa_E0032
sequence annotation: BBa_R0040
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_R0062
sequence annotation: BBa_B0034
sequence annotation: BBa_C0062
sequence: https://synbiohub.org/public/igem/BBa_I8035_sequence/1

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display id: component968871
version: 1
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access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

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version: 1
top level: BBa_I8035
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definition: https://synbiohub.org/public/igem/BBa_E0032/1

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display id: annotation968841
version: 1
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type: Range
persistent identity: range968841
display id: range968841
version: 1
direction: direction/forward
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start: 1070
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BBa_J06951
type: ComponentDefinition
persistent identity: BBa J06951
display id: BBa_J06951
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Lambda cI/LacI ts (mut 241) RS flip-flop
creator: ytwang
created: 2005-06-27T11:00:00Z
modified: 2015-08-31T04:08:19Z
discontinued: true
dominant: false
experience: experience/None
group u list: _20_
m user id: 0
owner id: 340
owning group id: 20
sample status: Discontinued
status: status/Deleted
bookmark: false
mutable description: RS flip-flop with LacI ts (mut 241) as the set and lambda cI as the reset. It will also take UV exposure as the set and heat (40°C) as the reset.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
component: BBa_B0010
component: BBa_B0034
component: BBa_R0011
component: BBa_E2060
component: BBa_J06500
component: BBa_B0012
component: BBa_R0051
component: BBa_C0051
sequence annotation: BBa_E2060
sequence annotation: BBa_B0012
sequence annotation: BBa_B0034
sequence annotation: BBa_C0051
sequence annotation: BBa_B0010
sequence annotation: BBa_R0051
sequence annotation: BBa_R0011
sequence annotation: BBa_J06500
sequence: https://synbiohub.org/public/igem/BBa_J06951_sequence/1

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definition: https://synbiohub.org/public/igem/BBa_B0034/1

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version: 1
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access: public
definition: https://synbiohub.org/public/igem/BBa_R0011/1

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version: 1
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definition: https://synbiohub.org/public/igem/BBa_E2060/1

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definition: https://synbiohub.org/public/igem/BBa_J06500/1

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definition: https://synbiohub.org/public/igem/BBa_B0034/1

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definition: https://synbiohub.org/public/igem/BBa_B0012/1

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persistent identity: component1551253
display id: component1551253
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definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0012
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persistent identity: component1551282
display id: component1551282
version: 1
top level: BBa_J06951
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creator: crackdots
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modified: 2015-08-31T04:08:11Z
discontinued: false
dominant: false
group u list: _1_
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sample status: Not in stock
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description: Prakash: Design 2
creator: Randy Rettberg
created: 2003-12-03T12:00:00Z
modified: 2015-08-31T04:07:41Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
owned by: james
owned by: myers
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part status: Released HQ 2013
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status: status/Available
bookmark: false
mutable description: -- No description --
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star: false
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was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: E. colibrator design 3, Polkadorks (I3410.I3200.E0422.I3510.I3401.I3411.I0460.I3100.I3400.I3300.I340
creator: Stephen Lee, Roshan Kumar, Joe Levine, Ziyan Chu (Polkadorks, IAP 2004)
created: 2004-01-29T12:00:00Z
modified: 2015-08-31T04:07:41Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: E. colibrator design 3 has the OR gate embedded within the switch via a double instance of I3400 Hk022cI switch input device. Design 3 has an aiiA mechanism turn on during the switch OFF state, as illustrated by I0460 AiiA device immediately following I3411 Lambda cI switch output device. This allows the OFF state to speed up reset of the system by degrading residual HSL once quorum threshold is reached.
mutable notes: The layout of E. colibrator (I3002) is designed to insulate the most sensitive devices, namely those in the switch that store state. The hk022cI switch output (ON) is insulated by appearing first. This output is then attached to the AspA attractant sender and the CFP reporter (I3410.I3200.E0422). The quorum receiver and its control of the lambda cI switch input (OFF) occur next (I3510.I3401) because leaky TIPS through this module will encourage the OFF state dicated by the downstream lambda cI switch output (I3411.I0460). Note that the OFF switch output leads to aiiA synthesis to promote HSL degradation and system reset in this design. The rest of the switch inputs occur downstream because they do not store state and therefore do not need to be as insulated from leaky TIPS. Lac trigger controls one hk022cI input (I3100.I3400), and Tar-EnvZ receptor (I3300 upstream ompC version) controls the other (I3300.I3400). The dual control forms the built-in OR gate in one of the switch inputs. Finally, the quorum sender device (I3500) is last because it is meant to be constitutively on at some basal level, so insulation is not required. Note: Depending on the efficacy of the OmpR promoter, this design can be implemented with any of I3300, I3301, I3302 (variations of Tar-EnvZ devices with different OmpR promoters). The default implementation uses I3300.
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BBa_I12059
type: ComponentDefinition
persistent identity: BBa I12059
display id: BBa_I12059
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: BL oscillator (I12036 based) with IPTG kickstart
creator: endelman
created: 2004-09-02T11:00:00Z
modified: 2015-08-31T04:07:31Z
discontinued: false
dominant: false
experience: experience/None
group u list: _3_
m user id: 0
owner id: 145
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Modified BL oscillator based on I12036. It can be "reset" by adding IPTG which stimulates production of the A protein (lambda cI) if used in LacIq strain. The B protein is 434 cI. The YFP-AAV reporter tracks the dynamics of the A protein.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
component: BBa_I12056
component: BBa_I12054
component: BBa_I12024
component: BBa_E0434
sequence annotation: BBa_I12056
sequence annotation: BBa_I12054
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sequence annotation: BBa_E0434
sequence: https://synbiohub.org/public/igem/BBa_I12059_sequence/1

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BBa_I12054
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BBa_I12024
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BBa_E0434
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display id: BBa_J05050
version: 1
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description: Counter
creator: ETH Zurich iGEM Team 2005
created: 2005-09-14T11:00:00Z
modified: 2015-08-31T04:08:15Z
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dominant: false
group u list: _34_
m user id: 0
owner id: 412
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bookmark: false
mutable description: This is the design of the counter.
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owned by: myers
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definition: https://synbiohub.org/public/igem/BBa_J05040/1

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description: Test: AraC trigger (I3102.E0422)
creator: Stephen Lee, Roshan Kumar, Joe Levine, Ziyan Chu (Polkadorks, IAP 2004)
created: 2004-01-28T12:00:00Z
modified: 2015-08-31T04:07:41Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Tests I3102 AraC trigger device by attaching TIPS output to CFP reporter.
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owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
component: BBa_B0010
component: BBa_C0080
component: BBa_B0034
component: BBa_E0022
component: BBa_B0012
component: BBa_R0080
component: BBa_R1075
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sequence annotation: BBa_B0012
sequence annotation: BBa_B0010
sequence annotation: BBa_E0022
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display id: BBa_J13009
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was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LacI repressed pcyA from synechosystis
creator: Jeff Tabor
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modified: 2015-08-31T04:08:29Z
discontinued: false
dominant: false
experience: experience/None
group u list: _37_
m user id: 0
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owning group id: 37
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
component: BBa_B0012
component: BBa_R0011
component: BBa_I15009
component: BBa_B0034
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sequence annotation: BBa_R0011
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BBa_B0012
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BBa_R0011
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BBa_I15009
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BBa_B0034
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definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0010
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version: 1
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definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0012
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BBa_B0010
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BBa_I15009
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BBa_I9108
type: ComponentDefinition
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display id: BBa_I9108
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was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: test (tp901-cI nor) I9107.I9106.I9106
creator: crackdots
created: 2004-01-27T12:00:00Z
modified: 2015-08-31T04:08:11Z
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dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
component: BBa_I0500
component: BBa_B0010
component: BBa_B0034
component: BBa_B0012
component: BBa_E0022
component: BBa_E0032
component: BBa_C0075
component: BBa_R0075
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sequence annotation: BBa_I0500
sequence annotation: BBa_E0022
sequence annotation: BBa_E0032
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BBa_I0500
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version: 1
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definition: https://synbiohub.org/public/igem/BBa_I0500/1

BBa_B0010
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definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0034
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definition: https://synbiohub.org/public/igem/BBa_B0034/1

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definition: https://synbiohub.org/public/igem/BBa_E0022/1

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definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_E0032
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definition: https://synbiohub.org/public/igem/BBa_B0034/1

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BBa_B0012
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BBa_B0010
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definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0012
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definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_I0500
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BBa_R0075
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BBa_B0034
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BBa_B0012
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BBa_E0022
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BBa_C0075
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BBa_B0012
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BBa_I0500
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BBa_E0022
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BBa_I0500
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BBa_E0032
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BBa_B0010
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BBa_I0500
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BBa_B0012
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BBa_B0010
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BBa_I0500
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BBa_B0034
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BBa_C0075
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type: Range
persistent identity: range995727
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BBa_C0075
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orientation: inline

BBa_B0012
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persistent identity: range995707
display id: range995707
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BBa_E0022
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BBa_R0075
type: SequenceAnnotation
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type: Range
persistent identity: range995632
display id: range995632
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direction: direction/forward
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orientation: inline

BBa_B0034
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display id: range995753
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BBa_B0012
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BBa_B0034
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BBa_B0034
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persistent identity: range995793
display id: range995793
version: 1
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end: 8551
orientation: inline

BBa_B0010
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component: BBa_B0010

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persistent identity: range995809
display id: range995809
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BBa_B0010
type: SequenceAnnotation
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persistent identity: range995657
display id: range995657
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direction: direction/forward
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start: 914
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BBa_B0012
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persistent identity: range995783
display id: range995783
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BBa_B0010
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persistent identity: range995697
display id: range995697
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BBa_B0034
type: SequenceAnnotation
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display id: annotation995677
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type: Range
persistent identity: range995677
display id: range995677
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BBa_I13454
type: ComponentDefinition
persistent identity: BBa I13454
display id: BBa_I13454
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Insertion site #2
creator: jkm
created: 2005-06-19T11:00:00Z
modified: 2015-08-31T04:07:34Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_
m user id: 0
owner id: 253
owning group id: 6
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Contains a XmaI site and an AvrII site. A separate (non-BB) part contains a complete BB site within XmaI/AvrII sites.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: XmaI site
sequence annotation: AvrII site
sequence: https://synbiohub.org/public/igem/BBa_I13454_sequence/1

XmaI site
type: SequenceAnnotation
persistent identity: annotation1526494
display id: annotation1526494
version: 1
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role: SO:0000110
role: feature/misc
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type: Range
persistent identity: range1526494
display id: range1526494
version: 1
direction: direction/forward
top level: BBa_I13454
start: 1
end: 6
orientation: inline

AvrII site
type: SequenceAnnotation
persistent identity: annotation1526495
display id: annotation1526495
version: 1
top level: BBa_I13454
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I13454/annotation1526495/range1526495/1

https://synbiohub.org/public/igem/BBa_I13454/annotation1526495/range1526495/1
type: Range
persistent identity: range1526495
display id: range1526495
version: 1
direction: direction/forward
top level: BBa_I13454
start: 47
end: 52
orientation: inline

BBa_I12018
type: ComponentDefinition
persistent identity: BBa I12018
display id: BBa_I12018
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Barkai-Leibler Relaxation Oscillator
creator: mcnamara
created: 2004-07-21T11:00:00Z
modified: 2015-08-31T04:07:31Z
discontinued: false
dominant: false
experience: experience/None
group u list: _3_
m user id: 0
owner id: 147
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Modified Design
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
component: BBa_I12015
component: BBa_I12016
component: BBa_I12017
sequence annotation: BBa_I12017
sequence annotation: BBa_I12016
sequence annotation: BBa_I12015
sequence: https://synbiohub.org/public/igem/BBa_I12018_sequence/1

BBa_I12015
type: Component
persistent identity: component2231591
display id: component2231591
version: 1
top level: BBa_I12018
access: public
definition: https://synbiohub.org/public/igem/BBa_I12015/1

BBa_I12016
type: Component
persistent identity: component2231609
display id: component2231609
version: 1
top level: BBa_I12018
access: public
definition: https://synbiohub.org/public/igem/BBa_I12016/1

BBa_I12017
type: Component
persistent identity: component2231620
display id: component2231620
version: 1
top level: BBa_I12018
access: public
definition: https://synbiohub.org/public/igem/BBa_I12017/1

BBa_I12017
type: SequenceAnnotation
persistent identity: annotation2231620
display id: annotation2231620
version: 1
top level: BBa_I12018
location: https://synbiohub.org/public/igem/BBa_I12018/annotation2231620/range2231620/1
component: BBa_I12017

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type: Range
persistent identity: range2231620
display id: range2231620
version: 1
direction: direction/reverse
top level: BBa_I12018
start: 1256
end: 2046
orientation: inline

BBa_I12016
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display id: annotation2231609
version: 1
top level: BBa_I12018
location: https://synbiohub.org/public/igem/BBa_I12018/annotation2231609/range2231609/1
component: BBa_I12016

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persistent identity: range2231609
display id: range2231609
version: 1
direction: direction/reverse
top level: BBa_I12018
start: 228
end: 1247
orientation: inline

BBa_I12015
type: SequenceAnnotation
persistent identity: annotation2231591
display id: annotation2231591
version: 1
top level: BBa_I12018
location: https://synbiohub.org/public/igem/BBa_I12018/annotation2231591/range2231591/1
component: BBa_I12015

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type: Range
persistent identity: range2231591
display id: range2231591
version: 1
direction: direction/reverse
top level: BBa_I12018
start: 1
end: 219
orientation: inline

BBa_J06953
type: ComponentDefinition
persistent identity: BBa J06953
display id: BBa_J06953
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: 434 cI/lambda cI RS flip-flop
creator: ytwang
created: 2005-06-27T11:00:00Z
modified: 2015-08-31T04:08:19Z
discontinued: false
dominant: false
experience: experience/None
group u list: _20_
m user id: 0
owner id: 340
owning group id: 20
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: RS flip-flop with lambda cI as the set and 434 cI as the reset.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
component: BBa_B0010
component: BBa_R0052
component: BBa_B0012
component: BBa_C0052
component: BBa_E2060
component: BBa_R0051
component: BBa_B0034
component: BBa_C0051
sequence annotation: BBa_B0012
sequence annotation: BBa_C0052
sequence annotation: BBa_E2060
sequence annotation: BBa_B0010
sequence annotation: BBa_B0034
sequence annotation: BBa_C0051
sequence annotation: BBa_R0051
sequence annotation: BBa_R0052
sequence: https://synbiohub.org/public/igem/BBa_J06953_sequence/1

BBa_B0010
type: Component
persistent identity: component1551506
display id: component1551506
version: 1
top level: BBa_J06953
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_R0052
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display id: component1551480
version: 1
top level: BBa_J06953
access: public
definition: https://synbiohub.org/public/igem/BBa_R0052/1

BBa_B0012
type: Component
persistent identity: component1551516
display id: component1551516
version: 1
top level: BBa_J06953
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_C0052
type: Component
persistent identity: component1551549
display id: component1551549
version: 1
top level: BBa_J06953
access: public
definition: https://synbiohub.org/public/igem/BBa_C0052/1

BBa_E2060
type: Component
persistent identity: component1551611
display id: component1551611
version: 1
top level: BBa_J06953
access: public
definition: https://synbiohub.org/public/igem/BBa_E2060/1

BBa_R0051
type: Component
persistent identity: component1551531
display id: component1551531
version: 1
top level: BBa_J06953
access: public
definition: https://synbiohub.org/public/igem/BBa_R0051/1

BBa_B0034
type: Component
persistent identity: component1551539
display id: component1551539
version: 1
top level: BBa_J06953
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0010
type: Component
persistent identity: component1551617
display id: component1551617
version: 1
top level: BBa_J06953
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0010
type: Component
persistent identity: component1551556
display id: component1551556
version: 1
top level: BBa_J06953
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_R0052
type: Component
persistent identity: component1551588
display id: component1551588
version: 1
top level: BBa_J06953
access: public
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description: 434 cI/lambda cI857 (E07700) RS flip-flop with EYFP
creator: Yves Wang
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modified: 2015-08-31T04:08:19Z
discontinued: true
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description: Test: AspA attractant sender (I3100.I3200.B0015)
creator: Stephen Lee, Roshan Kumar, Joe Levine, Ziyan Chu (Polkadorks, IAP 2004)
created: 2004-01-28T12:00:00Z
modified: 2015-08-31T04:07:41Z
discontinued: false
dominant: false
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description: Olmecs: Repressilator version 1 (HK022 cI)
creator: Olmecs 2003 IAP Team
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modified: 2015-08-31T04:07:42Z
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type: Range
persistent identity: range2234317
display id: range2234317
version: 1
direction: direction/reverse
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start: 1901
end: 1912
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2234280
display id: annotation2234280
version: 1
top level: BBa_I5200
location: https://synbiohub.org/public/igem/BBa_I5200/annotation2234280/range2234280/1
component: BBa_B0034

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persistent identity: range2234280
display id: range2234280
version: 1
direction: direction/reverse
top level: BBa_I5200
start: 64
end: 75
orientation: inline

BBa_B0011
type: SequenceAnnotation
persistent identity: annotation2234289
display id: annotation2234289
version: 1
top level: BBa_I5200
location: https://synbiohub.org/public/igem/BBa_I5200/annotation2234289/range2234289/1
component: BBa_B0011

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type: Range
persistent identity: range2234289
display id: range2234289
version: 1
direction: direction/forward
top level: BBa_I5200
start: 824
end: 869
orientation: inline

BBa_I13951
type: ComponentDefinition
persistent identity: BBa I13951
display id: BBa_I13951
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Exogenous AiiA Relaxation Oscillator Test Construct (I13942.I13943)
creator: robertb
created: 2004-08-22T11:00:00Z
modified: 2015-08-31T04:07:37Z
discontinued: false
dominant: false
experience: experience/None
group u list: _6_
m user id: 0
owner id: 159
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
component: BBa_C0061
component: BBa_B0034
component: BBa_B0010
component: BBa_B0012
component: BBa_I0500
component: BBa_E0034
component: BBa_R0062
component: BBa_E0024
component: BBa_C0060
sequence annotation: BBa_B0012
sequence annotation: BBa_C0060
sequence annotation: BBa_C0061
sequence annotation: BBa_B0010
sequence annotation: BBa_I0500
sequence annotation: BBa_B0034
sequence annotation: BBa_E0034
sequence annotation: BBa_E0024
sequence annotation: BBa_R0062
sequence: https://synbiohub.org/public/igem/BBa_I13951_sequence/1

BBa_C0061
type: Component
persistent identity: component1060215
display id: component1060215
version: 1
top level: BBa_I13951
access: public
definition: https://synbiohub.org/public/igem/BBa_C0061/1

BBa_B0034
type: Component
persistent identity: component1060142
display id: component1060142
version: 1
top level: BBa_I13951
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0034
type: Component
persistent identity: component1060205
display id: component1060205
version: 1
top level: BBa_I13951
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0010
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display id: component1060229
version: 1
top level: BBa_I13951
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0034
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persistent identity: component1060165
display id: component1060165
version: 1
top level: BBa_I13951
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0012
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display id: component1060182
version: 1
top level: BBa_I13951
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_I0500
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display id: component1060137
version: 1
top level: BBa_I13951
access: public
definition: https://synbiohub.org/public/igem/BBa_I0500/1

BBa_E0034
type: Component
persistent identity: component1060224
display id: component1060224
version: 1
top level: BBa_I13951
access: public
definition: https://synbiohub.org/public/igem/BBa_E0034/1

BBa_R0062
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persistent identity: component1060197
display id: component1060197
version: 1
top level: BBa_I13951
access: public
definition: https://synbiohub.org/public/igem/BBa_R0062/1

BBa_E0024
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persistent identity: component1060167
display id: component1060167
version: 1
top level: BBa_I13951
access: public
definition: https://synbiohub.org/public/igem/BBa_E0024/1

BBa_B0012
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display id: component1060239
version: 1
top level: BBa_I13951
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0034
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persistent identity: component1060222
display id: component1060222
version: 1
top level: BBa_I13951
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_C0060
type: Component
persistent identity: component1060157
display id: component1060157
version: 1
top level: BBa_I13951
access: public
definition: https://synbiohub.org/public/igem/BBa_C0060/1

BBa_B0010
type: Component
persistent identity: component1060172
display id: component1060172
version: 1
top level: BBa_I13951
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1060239
display id: annotation1060239
version: 1
top level: BBa_I13951
location: https://synbiohub.org/public/igem/BBa_I13951/annotation1060239/range1060239/1
component: BBa_B0012

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persistent identity: range1060239
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direction: direction/forward
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start: 4592
end: 4632
orientation: inline

BBa_C0060
type: SequenceAnnotation
persistent identity: annotation1060157
display id: annotation1060157
version: 1
top level: BBa_I13951
location: https://synbiohub.org/public/igem/BBa_I13951/annotation1060157/range1060157/1
component: BBa_C0060

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persistent identity: range1060157
display id: range1060157
version: 1
direction: direction/forward
top level: BBa_I13951
start: 1237
end: 2025
orientation: inline

BBa_C0061
type: SequenceAnnotation
persistent identity: annotation1060215
display id: annotation1060215
version: 1
top level: BBa_I13951
location: https://synbiohub.org/public/igem/BBa_I13951/annotation1060215/range1060215/1
component: BBa_C0061

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persistent identity: range1060215
display id: range1060215
version: 1
direction: direction/forward
top level: BBa_I13951
start: 3065
end: 3682
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1060172
display id: annotation1060172
version: 1
top level: BBa_I13951
location: https://synbiohub.org/public/igem/BBa_I13951/annotation1060172/range1060172/1
component: BBa_B0010

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type: Range
persistent identity: range1060172
display id: range1060172
version: 1
direction: direction/forward
top level: BBa_I13951
start: 2847
end: 2926
orientation: inline

BBa_I0500
type: SequenceAnnotation
persistent identity: annotation1060137
display id: annotation1060137
version: 1
top level: BBa_I13951
location: https://synbiohub.org/public/igem/BBa_I13951/annotation1060137/range1060137/1
component: BBa_I0500

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type: Range
persistent identity: range1060137
display id: range1060137
version: 1
direction: direction/forward
top level: BBa_I13951
start: 1
end: 1210
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1060165
display id: annotation1060165
version: 1
top level: BBa_I13951
location: https://synbiohub.org/public/igem/BBa_I13951/annotation1060165/range1060165/1
component: BBa_B0034

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type: Range
persistent identity: range1060165
display id: range1060165
version: 1
direction: direction/forward
top level: BBa_I13951
start: 2059
end: 2070
orientation: inline

BBa_E0034
type: SequenceAnnotation
persistent identity: annotation1060224
display id: annotation1060224
version: 1
top level: BBa_I13951
location: https://synbiohub.org/public/igem/BBa_I13951/annotation1060224/range1060224/1
component: BBa_E0034

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persistent identity: range1060224
display id: range1060224
version: 1
direction: direction/forward
top level: BBa_I13951
start: 3734
end: 4495
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1060205
display id: annotation1060205
version: 1
top level: BBa_I13951
location: https://synbiohub.org/public/igem/BBa_I13951/annotation1060205/range1060205/1
component: BBa_B0034

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type: Range
persistent identity: range1060205
display id: range1060205
version: 1
direction: direction/forward
top level: BBa_I13951
start: 3047
end: 3058
orientation: inline

BBa_E0024
type: SequenceAnnotation
persistent identity: annotation1060167
display id: annotation1060167
version: 1
top level: BBa_I13951
location: https://synbiohub.org/public/igem/BBa_I13951/annotation1060167/range1060167/1
component: BBa_E0024

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type: Range
persistent identity: range1060167
display id: range1060167
version: 1
direction: direction/forward
top level: BBa_I13951
start: 2077
end: 2838
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1060142
display id: annotation1060142
version: 1
top level: BBa_I13951
location: https://synbiohub.org/public/igem/BBa_I13951/annotation1060142/range1060142/1
component: BBa_B0034

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type: Range
persistent identity: range1060142
display id: range1060142
version: 1
direction: direction/forward
top level: BBa_I13951
start: 1219
end: 1230
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1060229
display id: annotation1060229
version: 1
top level: BBa_I13951
location: https://synbiohub.org/public/igem/BBa_I13951/annotation1060229/range1060229/1
component: BBa_B0010

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type: Range
persistent identity: range1060229
display id: range1060229
version: 1
direction: direction/forward
top level: BBa_I13951
start: 4504
end: 4583
orientation: inline

BBa_R0062
type: SequenceAnnotation
persistent identity: annotation1060197
display id: annotation1060197
version: 1
top level: BBa_I13951
location: https://synbiohub.org/public/igem/BBa_I13951/annotation1060197/range1060197/1
component: BBa_R0062

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type: Range
persistent identity: range1060197
display id: range1060197
version: 1
direction: direction/forward
top level: BBa_I13951
start: 2984
end: 3038
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1060182
display id: annotation1060182
version: 1
top level: BBa_I13951
location: https://synbiohub.org/public/igem/BBa_I13951/annotation1060182/range1060182/1
component: BBa_B0012

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type: Range
persistent identity: range1060182
display id: range1060182
version: 1
direction: direction/forward
top level: BBa_I13951
start: 2935
end: 2975
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1060222
display id: annotation1060222
version: 1
top level: BBa_I13951
location: https://synbiohub.org/public/igem/BBa_I13951/annotation1060222/range1060222/1
component: BBa_B0034

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type: Range
persistent identity: range1060222
display id: range1060222
version: 1
direction: direction/forward
top level: BBa_I13951
start: 3716
end: 3727
orientation: inline

BBa_I12039
type: ComponentDefinition
persistent identity: BBa I12039
display id: BBa_I12039
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Test construct for BBa_I12036
creator: ryhsiao
created: 2004-08-18T11:00:00Z
modified: 2015-08-31T04:07:31Z
discontinued: false
dominant: false
experience: experience/None
group u list: _3_
m user id: 0
owner id: 148
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Expression of lambda cI controlled by IPTG (requires LacIq strain). Expression of 434 cI controlled by arabinose. If BBa_I12036 promoter works, YFP is activated by lambda cI and repressed by 434 cI.
mutable notes: Designed in the exact same way as BBa_I12028 (Test construct for I12006) except with BBa_I12036 swapped in for BBa_I12006
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
component: BBa_E0430
component: BBa_I12027
component: BBa_I12036
component: BBa_I12024
sequence annotation: BBa_I12024
sequence annotation: BBa_I12027
sequence annotation: BBa_I12036
sequence annotation: BBa_E0430
sequence: https://synbiohub.org/public/igem/BBa_I12039_sequence/1

BBa_E0430
type: Component
persistent identity: component2235008
display id: component2235008
version: 1
top level: BBa_I12039
access: public
definition: https://synbiohub.org/public/igem/BBa_E0430/1

BBa_I12027
type: Component
persistent identity: component2234990
display id: component2234990
version: 1
top level: BBa_I12039
access: public
definition: https://synbiohub.org/public/igem/BBa_I12027/1

BBa_I12036
type: Component
persistent identity: component2234998
display id: component2234998
version: 1
top level: BBa_I12039
access: public
definition: https://synbiohub.org/public/igem/BBa_I12036/1

BBa_I12024
type: Component
persistent identity: component2234976
display id: component2234976
version: 1
top level: BBa_I12039
access: public
definition: https://synbiohub.org/public/igem/BBa_I12024/1

BBa_I12024
type: SequenceAnnotation
persistent identity: annotation2234976
display id: annotation2234976
version: 1
top level: BBa_I12039
location: https://synbiohub.org/public/igem/BBa_I12039/annotation2234976/range2234976/1
component: BBa_I12024

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type: Range
persistent identity: range2234976
display id: range2234976
version: 1
direction: direction/reverse
top level: BBa_I12039
start: 1
end: 993
orientation: inline

BBa_I12027
type: SequenceAnnotation
persistent identity: annotation2234990
display id: annotation2234990
version: 1
top level: BBa_I12039
location: https://synbiohub.org/public/igem/BBa_I12039/annotation2234990/range2234990/1
component: BBa_I12027

https://synbiohub.org/public/igem/BBa_I12039/annotation2234990/range2234990/1
type: Range
persistent identity: range2234990
display id: range2234990
version: 1
direction: direction/reverse
top level: BBa_I12039
start: 1002
end: 3068
orientation: inline

BBa_I12036
type: SequenceAnnotation
persistent identity: annotation2234998
display id: annotation2234998
version: 1
top level: BBa_I12039
location: https://synbiohub.org/public/igem/BBa_I12039/annotation2234998/range2234998/1
component: BBa_I12036

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type: Range
persistent identity: range2234998
display id: range2234998
version: 1
direction: direction/reverse
top level: BBa_I12039
start: 3077
end: 3167
orientation: inline

BBa_E0430
type: SequenceAnnotation
persistent identity: annotation2235008
display id: annotation2235008
version: 1
top level: BBa_I12039
location: https://synbiohub.org/public/igem/BBa_I12039/annotation2235008/range2235008/1
component: BBa_E0430

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type: Range
persistent identity: range2235008
display id: range2235008
version: 1
direction: direction/reverse
top level: BBa_I12039
start: 3176
end: 4053
orientation: inline

BBa_J11020
type: ComponentDefinition
persistent identity: BBa J11020
display id: BBa_J11020
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Tunable Temperature Sensor
creator: Emanuel Nazareth
created: 2005-09-28T11:00:00Z
modified: 2015-08-31T04:08:25Z
discontinued: false
dominant: false
group u list: _38_
m user id: 0
owner id: 354
owning group id: 38
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Tunable Temperature Sensor made with parts availble in Registry 7.05 DNA-1 and cI857ts.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
component: BBa_B0010
component: BBa_R0051
component: BBa_C0081
component: BBa_I12007
component: BBa_E0040
component: BBa_R0040
component: BBa_B0031
component: BBa_B0030
component: BBa_B0012
component: BBa_E1010
sequence annotation: BBa_B0012
sequence annotation: BBa_I12007
sequence annotation: BBa_C0081
sequence annotation: BBa_R0040
sequence annotation: BBa_E0040
sequence annotation: BBa_B0010
sequence annotation: BBa_R0051
sequence annotation: BBa_B0030
sequence annotation: BBa_E1010
sequence annotation: BBa_B0031
sequence: https://synbiohub.org/public/igem/BBa_J11020_sequence/1

BBa_B0010
type: Component
persistent identity: component1710516
display id: component1710516
version: 1
top level: BBa_J11020
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0010
type: Component
persistent identity: component1710626
display id: component1710626
version: 1
top level: BBa_J11020
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_R0051
type: Component
persistent identity: component1710501
display id: component1710501
version: 1
top level: BBa_J11020
access: public
definition: https://synbiohub.org/public/igem/BBa_R0051/1

BBa_C0081
type: Component
persistent identity: component1710563
display id: component1710563
version: 1
top level: BBa_J11020
access: public
definition: https://synbiohub.org/public/igem/BBa_C0081/1

BBa_I12007
type: Component
persistent identity: component1710546
display id: component1710546
version: 1
top level: BBa_J11020
access: public
definition: https://synbiohub.org/public/igem/BBa_I12007/1

BBa_E0040
type: Component
persistent identity: component1710511
display id: component1710511
version: 1
top level: BBa_J11020
access: public
definition: https://synbiohub.org/public/igem/BBa_E0040/1

BBa_B0010
type: Component
persistent identity: component1710576
display id: component1710576
version: 1
top level: BBa_J11020
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_C0081
type: Component
persistent identity: component1710621
display id: component1710621
version: 1
top level: BBa_J11020
access: public
definition: https://synbiohub.org/public/igem/BBa_C0081/1

BBa_R0040
type: Component
persistent identity: component1710601
display id: component1710601
version: 1
top level: BBa_J11020
access: public
definition: https://synbiohub.org/public/igem/BBa_R0040/1

BBa_B0031
type: Component
persistent identity: component1710509
display id: component1710509
version: 1
top level: BBa_J11020
access: public
definition: https://synbiohub.org/public/igem/BBa_B0031/1

BBa_B0030
type: Component
persistent identity: component1710554
display id: component1710554
version: 1
top level: BBa_J11020
access: public
definition: https://synbiohub.org/public/igem/BBa_B0030/1

BBa_B0012
type: Component
persistent identity: component1710526
display id: component1710526
version: 1
top level: BBa_J11020
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0012
type: Component
persistent identity: component1710648
display id: component1710648
version: 1
top level: BBa_J11020
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0012
type: Component
persistent identity: component1710636
display id: component1710636
version: 1
top level: BBa_J11020
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0030
type: Component
persistent identity: component1710612
display id: component1710612
version: 1
top level: BBa_J11020
access: public
definition: https://synbiohub.org/public/igem/BBa_B0030/1

BBa_E1010
type: Component
persistent identity: component1710570
display id: component1710570
version: 1
top level: BBa_J11020
access: public
definition: https://synbiohub.org/public/igem/BBa_E1010/1

BBa_B0012
type: Component
persistent identity: component1710586
display id: component1710586
version: 1
top level: BBa_J11020
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1710648
display id: annotation1710648
version: 1
top level: BBa_J11020
location: https://synbiohub.org/public/igem/BBa_J11020/annotation1710648/range1710648/1
component: BBa_B0012

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type: Range
persistent identity: range1710648
display id: range1710648
version: 1
direction: direction/forward
top level: BBa_J11020
start: 3639
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was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Lambda cI switch output device (R0050.B0034.C0051)
creator: Stephen Lee, Roshan Kumar, Joe Levine, Ziyan Chu (Polkadorks, IAP 2004)
created: 2004-01-28T12:00:00Z
modified: 2015-08-31T04:07:41Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: One output from the Hk022cI-lambda cI wired-nor bistable switch.
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was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Test: Switch response to aspartate input (I3410.E0422.I3411.E0432.I3100.I3400.I3300.I3400.I3102.I340
creator: Stephen Lee, Roshan Kumar, Joe Levine, Ziyan Chu (Polkadorks, IAP 2004)
created: 2004-01-28T12:00:00Z
modified: 2015-08-31T04:07:41Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Tests ability of switch to respond to aspartate input by connecting the Tar-EnvZ receptor (I3300 ompC upstream version) to one of the switch inputs. The remaining testbed is analogous to the switch functionality testbed (I3420).
mutable notes: The layout of the switch mimics the layout of the proposed Polkadorks E. colibrator (I3000). Two switch outputs are insulated by appearing first. Hk022cI is attached to the CFP reporter (I3410.E0422). Lambda cI is attached to the YFP reporter (I3411.E0432). The three individually controlled trigger inputs follow the outputs in the layout. Lac trigger controls one hk022cI input (I3100.I3400), and Tar-EnvZ receptor (I3300 upstream ompC version) controls the other (I3300.I3400). The dual control forms the built-in OR gate in one of the switch inputs. Finally, AraC trigger controls the lambda cI input (I3102.I3401).
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was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: 3OC6HSL + LuxR reporter and aiia tuning device
creator: Jeffrey J Tabor
created: 2005-09-19T11:00:00Z
modified: 2015-08-31T04:08:30Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _37_5_
m user id: 0
owner id: 88
owning group id: 37
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This part is analogous to J13202, but encodes a more stable aiia protein, with presumably more anti-3OC6HSL activity.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
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type: ComponentDefinition
persistent identity: BBa J13030
display id: BBa_J13030
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: AI-1 amplifier
creator: Laura Lavery
created: 2005-06-21T11:00:00Z
modified: 2015-08-31T04:08:29Z
discontinued: false
dominant: false
experience: experience/None
group u list: _37_
m user id: 0
owner id: 327
owning group id: 37
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This circuit is regulated by two proteins, TetR and CI. In the presence of TetR, LasR production will be repressed. In the presence of CI, LasI production will be repressed. Without repressors present the circuit may be leaky enough to trigger the positive feedback LasI/LasR/PAI response.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
component: BBa_B0010
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was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: 3OC6HSL + LuxR reporter and cI tuning device
creator: Jeffrey J Tabor
created: 2005-09-19T11:00:00Z
modified: 2015-08-31T04:08:30Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _37_5_
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sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This device uses the S03182 module to produce lambda cI in an IPTG (or lactose) dependent fashion. Lambda cI concentration should be tune-able in certain E.coli strains. The I6042 module is used to read out 3OC6HSL and LuxR concentrations.
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persistent identity: BBa I3600
display id: BBa_I3600
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Test: Chemotaxis response to secreted aspartate, sender cells (I3100.I3200.E0422)
creator: Stephen Lee, Roshan Kumar, Joe Levine, Ziyan Chu (Polkadorks, IAP 2004)
created: 2004-01-28T12:00:00Z
modified: 2015-08-31T04:07:41Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
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sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Tests the chemotaxis cell-cell signalling machinery based on aspartate secretions. Sender cells are nonmotile and have a trigger controlling the AspA attractant sender and CFP reporter indicating transcription of aspA. Receiver cells have a constitutive promoter controlling YFP reporter. Chemotaxis can be observed by the movement of the YFP-marked receiver cells.
mutable notes: Sender cells are the nonchemotactic E. coli strain RP5232. Receiver cells are wildtype E. coli.
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owned by: myers
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type: ComponentDefinition
persistent identity: BBa I9110
display id: BBa_I9110
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: test (lambda-cI nor_output) I9201.E0432
creator: crackdots
created: 2004-01-27T12:00:00Z
modified: 2015-08-31T04:08:11Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
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m user id: 0
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status: status/Planning
bookmark: false
mutable description: -- No description --
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bookmark: false
mutable description: -- No description --
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star: false
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end: 5282
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BBa_B0032
type: SequenceAnnotation
persistent identity: annotation1004570
display id: annotation1004570
version: 1
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location: https://synbiohub.org/public/igem/BBa_I4003/annotation1004570/range1004570/1
component: BBa_B0032

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persistent identity: range1004570
display id: range1004570
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top level: BBa_I4003
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end: 981
orientation: inline

BBa_R0065
type: SequenceAnnotation
persistent identity: annotation1004737
display id: annotation1004737
version: 1
top level: BBa_I4003
location: https://synbiohub.org/public/igem/BBa_I4003/annotation1004737/range1004737/1
component: BBa_R0065

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persistent identity: range1004737
display id: range1004737
version: 1
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BBa_I3701
type: ComponentDefinition
persistent identity: BBa I3701
display id: BBa_I3701
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: AraC input device for OR gate (B0034.C0080.B0015)
creator: Stephen Lee, Roshan Kumar, Joe Levine, Ziyan Chu (Polkadorks, IAP 2004)
created: 2004-01-29T12:00:00Z
modified: 2015-08-31T04:07:41Z
discontinued: false
dominant: true
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This input device takes TIPS in as one of two OR gate inputs and produces AraC. AraC then acts on the araC promoter in I3700 to complete the OR gate.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
component: BBa_B0034
component: BBa_B0012
component: BBa_C0080
component: BBa_B0010
sequence annotation: BBa_B0034
sequence annotation: BBa_C0080
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_I3701_sequence/1

BBa_B0034
type: Component
persistent identity: component943737
display id: component943737
version: 1
top level: BBa_I3701
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0012
type: Component
persistent identity: component943763
display id: component943763
version: 1
top level: BBa_I3701
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_C0080
type: Component
persistent identity: component943747
display id: component943747
version: 1
top level: BBa_I3701
access: public
definition: https://synbiohub.org/public/igem/BBa_C0080/1

BBa_B0010
type: Component
persistent identity: component943753
display id: component943753
version: 1
top level: BBa_I3701
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation943737
display id: annotation943737
version: 1
top level: BBa_I3701
location: https://synbiohub.org/public/igem/BBa_I3701/annotation943737/range943737/1
component: BBa_B0034

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persistent identity: range943737
display id: range943737
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orientation: inline

BBa_C0080
type: SequenceAnnotation
persistent identity: annotation943747
display id: annotation943747
version: 1
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location: https://synbiohub.org/public/igem/BBa_I3701/annotation943747/range943747/1
component: BBa_C0080

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display id: range943747
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direction: direction/forward
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orientation: inline

BBa_B0010
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component: BBa_B0010

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direction: direction/forward
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orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation943763
display id: annotation943763
version: 1
top level: BBa_I3701
location: https://synbiohub.org/public/igem/BBa_I3701/annotation943763/range943763/1
component: BBa_B0012

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type: Range
persistent identity: range943763
display id: range943763
version: 1
direction: direction/forward
top level: BBa_I3701
start: 1055
end: 1095
orientation: inline

BBa_I3310
type: ComponentDefinition
persistent identity: BBa I3310
display id: BBa_I3310
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Test: Tar-EnvZ attractant receiver (I3300.E0422)
creator: Stephen Lee, Roshan Kumar, Joe Levine, Ziyan Chu (Polkadorks, IAP 2004)
created: 2004-01-28T12:00:00Z
modified: 2015-08-31T04:07:41Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Tests Tar-EnvZ attractant receiver device (ompC upstream version) by attaching TIPS output to CFP reporter.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
component: BBa_B0012
component: BBa_B0034
component: BBa_R0082
component: BBa_R1075
component: BBa_C0082
component: BBa_E0022
component: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_E0022
sequence annotation: BBa_B0010
sequence annotation: BBa_C0082
sequence annotation: BBa_B0034
sequence annotation: BBa_R0082
sequence annotation: BBa_R1075
sequence: https://synbiohub.org/public/igem/BBa_I3310_sequence/1

BBa_B0012
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persistent identity: component968340
display id: component968340
version: 1
top level: BBa_I3310
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0034
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display id: component968253
version: 1
top level: BBa_I3310
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0034
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display id: component968310
version: 1
top level: BBa_I3310
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

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persistent identity: component968305
display id: component968305
version: 1
top level: BBa_I3310
access: public
definition: https://synbiohub.org/public/igem/BBa_R0082/1

BBa_R1075
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display id: component968248
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access: public
definition: https://synbiohub.org/public/igem/BBa_R1075/1

BBa_C0082
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display id: component968269
version: 1
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access: public
definition: https://synbiohub.org/public/igem/BBa_C0082/1

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version: 1
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definition: https://synbiohub.org/public/igem/BBa_E0022/1

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display id: component968330
version: 1
top level: BBa_I3310
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0012
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display id: component968285
version: 1
top level: BBa_I3310
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0010
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display id: component968275
version: 1
top level: BBa_I3310
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

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display id: annotation968340
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orientation: inline

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version: 1
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location: https://synbiohub.org/public/igem/BBa_I3310/annotation968322/range968322/1
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persistent identity: range968322
display id: range968322
version: 1
direction: direction/forward
top level: BBa_I3310
start: 1871
end: 2632
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation968330
display id: annotation968330
version: 1
top level: BBa_I3310
location: https://synbiohub.org/public/igem/BBa_I3310/annotation968330/range968330/1
component: BBa_B0010

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display id: range968330
version: 1
direction: direction/forward
top level: BBa_I3310
start: 2641
end: 2720
orientation: inline

BBa_C0082
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display id: annotation968269
version: 1
top level: BBa_I3310
location: https://synbiohub.org/public/igem/BBa_I3310/annotation968269/range968269/1
component: BBa_C0082

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persistent identity: range968269
display id: range968269
version: 1
direction: direction/forward
top level: BBa_I3310
start: 76
end: 1566
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation968310
display id: annotation968310
version: 1
top level: BBa_I3310
location: https://synbiohub.org/public/igem/BBa_I3310/annotation968310/range968310/1
component: BBa_B0034

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persistent identity: range968310
display id: range968310
version: 1
direction: direction/forward
top level: BBa_I3310
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end: 1864
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation968275
display id: annotation968275
version: 1
top level: BBa_I3310
location: https://synbiohub.org/public/igem/BBa_I3310/annotation968275/range968275/1
component: BBa_B0010

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persistent identity: range968275
display id: range968275
version: 1
direction: direction/forward
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start: 1600
end: 1679
orientation: inline

BBa_B0012
type: SequenceAnnotation
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display id: annotation968285
version: 1
top level: BBa_I3310
location: https://synbiohub.org/public/igem/BBa_I3310/annotation968285/range968285/1
component: BBa_B0012

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persistent identity: range968285
display id: range968285
version: 1
direction: direction/forward
top level: BBa_I3310
start: 1688
end: 1728
orientation: inline

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type: SequenceAnnotation
persistent identity: annotation968305
display id: annotation968305
version: 1
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location: https://synbiohub.org/public/igem/BBa_I3310/annotation968305/range968305/1
component: BBa_R0082

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type: Range
persistent identity: range968305
display id: range968305
version: 1
direction: direction/forward
top level: BBa_I3310
start: 1737
end: 1844
orientation: inline

BBa_R1075
type: SequenceAnnotation
persistent identity: annotation968248
display id: annotation968248
version: 1
top level: BBa_I3310
location: https://synbiohub.org/public/igem/BBa_I3310/annotation968248/range968248/1
component: BBa_R1075

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type: Range
persistent identity: range968248
display id: range968248
version: 1
direction: direction/forward
top level: BBa_I3310
start: 1
end: 49
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation968253
display id: annotation968253
version: 1
top level: BBa_I3310
location: https://synbiohub.org/public/igem/BBa_I3310/annotation968253/range968253/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I3310/annotation968253/range968253/1
type: Range
persistent identity: range968253
display id: range968253
version: 1
direction: direction/forward
top level: BBa_I3310
start: 58
end: 69
orientation: inline

BBa_I12028
type: ComponentDefinition
persistent identity: BBa I12028
display id: BBa_I12028
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Test construct for I12006
creator: endelman
created: 2004-07-27T11:00:00Z
modified: 2015-08-31T04:07:31Z
discontinued: false
dominant: false
experience: experience/None
group u list: _3_
m user id: 0
owner id: 145
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Expression of lambda cI controlled by IPTG (requires LacIq strain). Expression of 434 cI controlled by arabinose. If I12006 promoter works, YFP is activated by lambda cI and repressed by 434 cI.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
component: BBa_E0430
component: BBa_I12027
component: BBa_I12006
component: BBa_I12024
sequence annotation: BBa_I12027
sequence annotation: BBa_I12024
sequence annotation: BBa_E0430
sequence annotation: BBa_I12006
sequence: https://synbiohub.org/public/igem/BBa_I12028_sequence/1

BBa_E0430
type: Component
persistent identity: component2234805
display id: component2234805
version: 1
top level: BBa_I12028
access: public
definition: https://synbiohub.org/public/igem/BBa_E0430/1

BBa_I12027
type: Component
persistent identity: component2234789
display id: component2234789
version: 1
top level: BBa_I12028
access: public
definition: https://synbiohub.org/public/igem/BBa_I12027/1

BBa_I12006
type: Component
persistent identity: component2234795
display id: component2234795
version: 1
top level: BBa_I12028
access: public
definition: https://synbiohub.org/public/igem/BBa_I12006/1

BBa_I12024
type: Component
persistent identity: component2234775
display id: component2234775
version: 1
top level: BBa_I12028
access: public
definition: https://synbiohub.org/public/igem/BBa_I12024/1

BBa_I12027
type: SequenceAnnotation
persistent identity: annotation2234789
display id: annotation2234789
version: 1
top level: BBa_I12028
location: https://synbiohub.org/public/igem/BBa_I12028/annotation2234789/range2234789/1
component: BBa_I12027

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type: Range
persistent identity: range2234789
display id: range2234789
version: 1
direction: direction/reverse
top level: BBa_I12028
start: 1002
end: 3068
orientation: inline

BBa_I12024
type: SequenceAnnotation
persistent identity: annotation2234775
display id: annotation2234775
version: 1
top level: BBa_I12028
location: https://synbiohub.org/public/igem/BBa_I12028/annotation2234775/range2234775/1
component: BBa_I12024

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type: Range
persistent identity: range2234775
display id: range2234775
version: 1
direction: direction/reverse
top level: BBa_I12028
start: 1
end: 993
orientation: inline

BBa_E0430
type: SequenceAnnotation
persistent identity: annotation2234805
display id: annotation2234805
version: 1
top level: BBa_I12028
location: https://synbiohub.org/public/igem/BBa_I12028/annotation2234805/range2234805/1
component: BBa_E0430

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type: Range
persistent identity: range2234805
display id: range2234805
version: 1
direction: direction/reverse
top level: BBa_I12028
start: 3167
end: 4044
orientation: inline

BBa_I12006
type: SequenceAnnotation
persistent identity: annotation2234795
display id: annotation2234795
version: 1
top level: BBa_I12028
location: https://synbiohub.org/public/igem/BBa_I12028/annotation2234795/range2234795/1
component: BBa_I12006

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type: Range
persistent identity: range2234795
display id: range2234795
version: 1
direction: direction/reverse
top level: BBa_I12028
start: 3077
end: 3158
orientation: inline

BBa_J13010
type: ComponentDefinition
persistent identity: BBa J13010
display id: BBa_J13010
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LacI repressed Cph8
creator: Jeff Tabor
created: 2005-07-17T11:00:00Z
modified: 2015-08-31T04:08:29Z
discontinued: false
dominant: false
experience: experience/None
group u list: _37_
m user id: 0
owner id: 88
owning group id: 37
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
component: BBa_B0010
component: BBa_B0034
component: BBa_I15010
component: BBa_B0012
component: BBa_R0011
sequence annotation: BBa_I15010
sequence annotation: BBa_R0011
sequence annotation: BBa_B0012
sequence annotation: BBa_B0010
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_J13010_sequence/1

BBa_B0010
type: Component
persistent identity: component1584361
display id: component1584361
version: 1
top level: BBa_J13010
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0034
type: Component
persistent identity: component1584351
display id: component1584351
version: 1
top level: BBa_J13010
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_I15010
type: Component
persistent identity: component1584355
display id: component1584355
version: 1
top level: BBa_J13010
access: public
definition: https://synbiohub.org/public/igem/BBa_I15010/1

BBa_B0012
type: Component
persistent identity: component1584371
display id: component1584371
version: 1
top level: BBa_J13010
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_R0011
type: Component
persistent identity: component1584342
display id: component1584342
version: 1
top level: BBa_J13010
access: public
definition: https://synbiohub.org/public/igem/BBa_R0011/1

BBa_I15010
type: SequenceAnnotation
persistent identity: annotation1584355
display id: annotation1584355
version: 1
top level: BBa_J13010
location: https://synbiohub.org/public/igem/BBa_J13010/annotation1584355/range1584355/1
component: BBa_I15010

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persistent identity: range1584355
display id: range1584355
version: 1
direction: direction/forward
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end: 2319
orientation: inline

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type: SequenceAnnotation
persistent identity: annotation1584342
display id: annotation1584342
version: 1
top level: BBa_J13010
location: https://synbiohub.org/public/igem/BBa_J13010/annotation1584342/range1584342/1
component: BBa_R0011

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persistent identity: range1584342
display id: range1584342
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direction: direction/forward
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start: 1
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orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1584371
display id: annotation1584371
version: 1
top level: BBa_J13010
location: https://synbiohub.org/public/igem/BBa_J13010/annotation1584371/range1584371/1
component: BBa_B0012

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display id: range1584371
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direction: direction/forward
top level: BBa_J13010
start: 2441
end: 2481
orientation: inline

BBa_B0010
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persistent identity: annotation1584361
display id: annotation1584361
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component: BBa_B0010

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persistent identity: range1584361
display id: range1584361
version: 1
direction: direction/forward
top level: BBa_J13010
start: 2353
end: 2432
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1584351
display id: annotation1584351
version: 1
top level: BBa_J13010
location: https://synbiohub.org/public/igem/BBa_J13010/annotation1584351/range1584351/1
component: BBa_B0034

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type: Range
persistent identity: range1584351
display id: range1584351
version: 1
direction: direction/forward
top level: BBa_J13010
start: 64
end: 75
orientation: inline

BBa_I3312
type: ComponentDefinition
persistent identity: BBa I3312
display id: BBa_I3312
version: 1
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description: Test: Tar-EnvZ attractant receiver (I3302.E0422)
creator: Stephen Lee, Roshan Kumar, Joe Levine, Ziyan Chu (Polkadorks, IAP 2004)
created: 2004-01-28T12:00:00Z
modified: 2015-08-31T04:07:41Z
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group u list: _1_
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sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Tests Tar-EnvZ attractant receiver device (ompF upstream version) by attaching TIPS output to CFP reporter.
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was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Test: Quorum threshold level and chemotaxis response, sender cells (I3600.I3520.I3500)
creator: Stephen Lee, Roshan Kumar, Joe Levine, Ziyan Chu (Polkadorks, IAP 2004)
created: 2004-01-28T12:00:00Z
modified: 2015-08-31T04:07:41Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
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bookmark: false
mutable description: Tests cellular density of asparate-secreting cells needed to induce quorum threshold response. Sender cells are nonmotile and have a trigger controlling the AspA attractant sender and CFP reporter indicating transcription of aspA. Receiver cells have a constitutive promoter controlling YFP reporter. Chemotaxis can be observed by the movement of the YFP-marked receiver cells. Quorum threshold is detected when the LacZ reporter is observed.
mutable notes: Sender cells are the nonchemotactic E. coli strain RP5232. Receiver cells are wildtype E. coli. This test combines the chemotaxis response test with the quorum threshold test.
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was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Hk022cI switch output device (R0051.B0034.C0050)
creator: Stephen Lee, Roshan Kumar, Joe Levine, Ziyan Chu (Polkadorks, IAP 2004)
created: 2004-01-28T12:00:00Z
modified: 2015-08-31T04:07:41Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: One output from the Hk022cI-lambda cI wired-nor bistable switch.
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was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Test: Switch aspartate excretion (I3410.I3200.I3411.E0432.I3100.I3400.I3101.I3400.I3102.I3401)
creator: Stephen Lee, Roshan Kumar, Joe Levine, Ziyan Chu (Polkadorks, IAP 2004)
created: 2004-01-28T12:00:00Z
modified: 2015-08-31T04:07:41Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Tests ability of switch to output aspartate given particular inputs. The remaining testbed is analogous to the switch functionality testbed (I3420).
mutable notes: The layout of the switch mimics the layout of the proposed Polkadorks E. colibrator (I3000). Two switch outputs are insulated by appearing first. Hk022cI is attached to the AspA attractant sender (I3410.I3200). Lambda cI is attached to the YFP reporter (I3411.E0432). The three individually controlled trigger inputs follow the outputs in the layout. Lac trigger controls one hk022cI input (I3100.I3400), and TetR trigger controls the other (I3101.I3400). The dual control forms the built-in OR gate in one of the switch inputs. Finally, AraC trigger controls the lambda cI input (I3102.I3401).
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BBa_R0050
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BBa_R0051
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BBa_B0034
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BBa_R1075
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BBa_B0034
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BBa_R1075
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BBa_B0010
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BBa_B0034
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BBa_B0010
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BBa_B0010
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BBa_B0034
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BBa_B0010
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BBa_C0051
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BBa_B0012
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BBa_E0032
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BBa_C0080
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BBa_C0040
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BBa_B0034
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BBa_B0034
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BBa_C0012
type: SequenceAnnotation
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BBa_C0050
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BBa_B0012
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https://synbiohub.org/public/igem/BBa_I3425/annotation1006251/range1006251/1
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BBa_B0012
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BBa_B0034
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BBa_B0034
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BBa_B0034
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BBa_C0050
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BBa_R0080
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BBa_R1075
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BBa_B0012
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BBa_C0051
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BBa_C0083
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BBa_R0040
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BBa_R0010
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BBa_B0010
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BBa_B0034
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BBa_B0012
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BBa_B0010
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BBa_C0050
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BBa_B0012
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BBa_B0012
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BBa_I2015
type: ComponentDefinition
persistent identity: BBa I2015
display id: BBa_I2015
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Unnatural Selector Revised
creator: cconboy
created: 2004-05-24T11:00:00Z
modified: 2015-08-31T04:07:39Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_1_
m user id: 0
owner id: 60
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: R1075 was replaced with R0053, a medium-strength promoter. Trigger component added.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
component: BBa_R0053
component: BBa_P0470
component: BBa_Q04800
component: BBa_P0480
component: BBa_R0051
component: BBa_P0478
component: BBa_E0433
component: BBa_B0034
component: BBa_P0479
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created: 2004-01-28T12:00:00Z
modified: 2015-08-31T04:07:41Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Tests I3100 Lac trigger device by attaching TIPS output to CFP reporter.
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description: LasI/LasR Signal Test (I2023.I2024)
creator: Sara Neves, Chris Walsh, Alvin Carter Powers (Fighting Darwins)
created: 2004-01-27T12:00:00Z
modified: 2015-08-31T04:07:39Z
discontinued: false
dominant: false
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status: status/Unavailable
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mutable description: lasI/lasR signal test: test lasR receiver when receiving signal from lasI sender (ie. test how they work together)
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description: Test: Switch response to quorum (I3410.E0422.I3411.E0432.I3100.I3400.I3101.I3400.I3510.I3401)
creator: Stephen Lee, Roshan Kumar, Joe Levine, Ziyan Chu (Polkadorks, IAP 2004)
created: 2004-01-28T12:00:00Z
modified: 2015-08-31T04:07:41Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Tests ability of switch to respond to quorum sensing input by connecting the quorum receiver to one of the switch inputs. The remaining testbed is analogous to the switch functionality testbed (I3420).
mutable notes: The layout of the switch mimics the layout of the proposed Polkadorks E. colibrator (I3000). Two switch outputs are insulated by appearing first. Hk022cI is attached to the CFP reporter (I3410.E0422). Lambda cI is attached to the YFP reporter (I3411.E0432). The three individually controlled trigger inputs follow the outputs in the layout. Lac trigger controls one hk022cI input (I3100.I3400), and TetR trigger controls the other (I3101.I3400). The dual control forms the built-in OR gate in one of the switch inputs. Finally, the quorum receiver device controls the lambda cI input (I3510.I3401).
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group u list: _1_
m user id: 0
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description: Tar-EnvZ attractant receiver device (R1075.B0034.C0082.B0015.R0083)
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description: penI QPI intermediate
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group u list: _6_
m user id: 0
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was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Synchronator 2 w/ CFP under control of Lac (I4202.I13601)
creator: robertb
created: 2004-08-09T11:00:00Z
modified: 2015-08-31T04:07:37Z
discontinued: true
dominant: false
experience: experience/None
group u list: _6_
m user id: 0
owner id: 159
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sample status: Discontinued
status: status/Deleted
bookmark: false
mutable description: This is the Synchronator Design 2 with an additional CFP generator under control of the Lac promoter for debug purposes.
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owned by: myers
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description: Lambda cI/LacI RS flip-flop
creator: ytwang
created: 2005-06-27T11:00:00Z
modified: 2015-08-31T04:08:19Z
discontinued: false
dominant: false
experience: experience/None
group u list: _20_
m user id: 0
owner id: 340
owning group id: 20
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: RS flip-flop with LacI as the set and lambda cI as the reset.
owned by: james
owned by: myers
star: false
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display id: BBa_J06954
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was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: 434 cI/lambda cI857 (E07700) RS flip-flop
creator: ytwang
created: 2005-06-27T11:00:00Z
modified: 2015-08-31T04:08:19Z
discontinued: true
dominant: false
experience: experience/None
group u list: _20_
m user id: 0
owner id: 340
owning group id: 20
sample status: Discontinued
status: status/Deleted
bookmark: false
mutable description: RS flip-flop with lambda cI (E07700) as the set and 434 cI as the reset. It will also take UV exposure as the set and heat (40??C) as the reset.
mutable notes: Cancelled due to failure to successfully mutate BBa_J06502 and BBa_J06802.
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owned by: myers
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BBa_I3001
type: ComponentDefinition
persistent identity: BBa I3001
display id: BBa_I3001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: E. colibrator design 2, Polkadorks (I3410.I3200.E0422.I3510.I3401.I3411.B0015.I3100.I3701.I3300.I370
creator: Stephen Lee, Roshan Kumar, Joe Levine, Ziyan Chu (Polkadorks, IAP 2004)
created: 2004-01-29T12:00:00Z
modified: 2015-08-31T04:07:41Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: E. colibrator design 2 has an explicit OR gate before the switch via an araC OR gate device (I3701, I3700). Design 2 has no explicit activity during the switch OFF state, as illustrated by the terminator immediately following I3411 Lambda cI switch output device. This causes the OFF state to rely completely on cellular diffusion to reset the system.
mutable notes: The layout of E. colibrator (I3001) is designed to insulate the most sensitive devices, namely those in the switch that store state. The hk022cI switch output (ON) is insulated by appearing first. This output is then attached to the AspA attractant sender and the CFP reporter (I3410.I3200.E0422). The quorum receiver and its control of the lambda cI switch input (OFF) occur next (I3510.I3401) because leaky TIPS through this module will encourage the OFF state dicated by the downstream lambda cI switch output (I3411.B0015). Note that the OFF switch output simply ends at termination of the output TIPS (B0015) in this design. The rest of the switch inputs occur downstream because they do not store state and therefore do not need to be as insulated from leaky TIPS. Lac trigger controls the araC input device for the OR gate (I3100.I3701), and Tar-EnvZ receptor (I3300 upstream ompC version) controls the second OR input, embedded in the araC buffer device (I3300.I3700). The araC OR gate in turn outputs TIPS into the hk022cI switch input (I3400). Finally, the quorum sender device (I3500) is last because it is meant to be constitutively on at some basal level, so insulation is not required. Note: Depending on the efficacy of the OmpR promoter, this design can be implemented with any of I3300, I3301, I3302 (variations of Tar-EnvZ devices with different OmpR promoters). The default implementation uses I3300.
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owned by: myers
star: false
type: DnaRegion
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BBa_B0034
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BBa_B0010
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BBa_R0040
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BBa_B0012
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BBa_B0010
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BBa_E0032
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BBa_B0010
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BBa_B0010
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BBa_B0034
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BBa_B0012
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BBa_B0012
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BBa_B0010
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BBa_B0012
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BBa_B0010
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BBa_B0012
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BBa_B0012
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BBa_B0010
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BBa_B0012
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description: AiiA (+LVA) Tri-part (B0031.C0060.B0015)
creator: robertb
created: 2004-08-03T11:00:00Z
modified: 2015-08-31T04:07:37Z
discontinued: false
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experience: experience/None
group u list: _6_
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status: status/Planning
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description: Olmecs *: Dualator part 2
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was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Test: Switch response to aspartate input (I3410.E0422.I3411.E0432.I3100.I3400.I3302.I3400.I3102.I340
creator: Stephen Lee, Roshan Kumar, Joe Levine, Ziyan Chu (Polkadorks, IAP 2004)
created: 2004-01-28T12:00:00Z
modified: 2015-08-31T04:07:41Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Tests ability of switch to respond to aspartate input by connecting the Tar-EnvZ receptor (I3302 ompF upstream version) to one of the switch inputs. The remaining testbed is analogous to the switch functionality testbed (I3420).
mutable notes: The layout of the switch mimics the layout of the proposed Polkadorks E. colibrator (I3000). Two switch outputs are insulated by appearing first. Hk022cI is attached to the CFP reporter (I3410.E0422). Lambda cI is attached to the YFP reporter (I3411.E0432). The three individually controlled trigger inputs follow the outputs in the layout. Lac trigger controls one hk022cI input (I3100.I3400), and Tar-EnvZ receptor (I3302 upstream ompF version) controls the other (I3302.I3400). The dual control forms the built-in OR gate in one of the switch inputs. Finally, AraC trigger controls the lambda cI input (I3102.I3401).
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BBa_B0010
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BBa_C0080
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BBa_E0022
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BBa_B0010
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BBa_B0012
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BBa_C0050
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BBa_B0012
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BBa_B0012
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BBa_B0012
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BBa_B0034
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BBa_B0012
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BBa_R0080
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BBa_B0012
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BBa_C0012
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BBa_B0034
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persistent identity: annotation1006463
display id: annotation1006463
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display id: range1006463
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BBa_R0084
type: SequenceAnnotation
persistent identity: annotation1006564
display id: annotation1006564
version: 1
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display id: range1006564
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BBa_B0034
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BBa_B0010
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display id: annotation1006634
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persistent identity: range1006634
display id: range1006634
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orientation: inline

BBa_B0034
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display id: annotation1006569
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display id: range1006569
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BBa_B0010
type: SequenceAnnotation
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display id: annotation1006688
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component: BBa_B0010

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type: Range
persistent identity: range1006688
display id: range1006688
version: 1
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end: 11061
orientation: inline

BBa_B0034
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persistent identity: annotation1006364
display id: annotation1006364
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persistent identity: range1006364
display id: range1006364
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BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1006544
display id: annotation1006544
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location: https://synbiohub.org/public/igem/BBa_I3423/annotation1006544/range1006544/1
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persistent identity: range1006544
display id: range1006544
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BBa_C0050
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display id: annotation1006584
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display id: range1006584
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BBa_R0050
type: SequenceAnnotation
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display id: annotation1006338
version: 1
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persistent identity: range1006338
display id: range1006338
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BBa_I12042
type: ComponentDefinition
persistent identity: BBa I12042
display id: BBa_I12042
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: BL oscillator, no cooperativity, reporter protein, kickstart
creator: Hans
created: 2004-08-19T11:00:00Z
modified: 2015-08-31T04:07:31Z
discontinued: false
dominant: false
experience: experience/None
group u list: _3_
m user id: 0
owner id: 103
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This part is a Barkai-Leibler oscillator with no cooperativity for the repressor (one 434 cI OR site). A reporter protein (EYFP) and a kickstart is included.
mutable notes: There is no cooperativity for 434 cI in this oscillator. A protein EYFP (with degradation tag) coding region is placed directly after the lambda cI coding region in order to keep track of the lambda cI levels. This part also includes a "kickstart" that will yield lambda cI if ATC is added.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
component: BBa_I12043
component: BBa_I12038
component: BBa_I12002
sequence annotation: BBa_I12002
sequence annotation: BBa_I12038
sequence annotation: BBa_I12043
sequence: https://synbiohub.org/public/igem/BBa_I12042_sequence/1

BBa_I12043
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persistent identity: component2238925
display id: component2238925
version: 1
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access: public
definition: https://synbiohub.org/public/igem/BBa_I12043/1

BBa_I12038
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display id: component2238886
version: 1
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access: public
definition: BBa_I12038

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persistent identity: component2238946
display id: component2238946
version: 1
top level: BBa_I12042
access: public
definition: https://synbiohub.org/public/igem/BBa_I12002/1

BBa_I12002
type: SequenceAnnotation
persistent identity: annotation2238946
display id: annotation2238946
version: 1
top level: BBa_I12042
location: https://synbiohub.org/public/igem/BBa_I12042/annotation2238946/range2238946/1
component: BBa_I12002

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persistent identity: range2238946
display id: range2238946
version: 1
direction: direction/reverse
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end: 4128
orientation: inline

BBa_I12038
type: SequenceAnnotation
persistent identity: annotation2238886
display id: annotation2238886
version: 1
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location: https://synbiohub.org/public/igem/BBa_I12042/annotation2238886/range2238886/1
component: BBa_I12038

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persistent identity: range2238886
display id: range2238886
version: 1
direction: direction/reverse
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end: 992
orientation: inline

BBa_I12043
type: SequenceAnnotation
persistent identity: annotation2238925
display id: annotation2238925
version: 1
top level: BBa_I12042
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component: BBa_I12043

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persistent identity: range2238925
display id: range2238925
version: 1
direction: direction/reverse
top level: BBa_I12042
start: 1001
end: 3102
orientation: inline

BBa_J13031
type: ComponentDefinition
persistent identity: BBa J13031
display id: BBa_J13031
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: 3OC6HSL and AI-1 amplifier
creator: Jeff Tabor
created: 2005-06-28T11:00:00Z
modified: 2015-08-31T04:08:29Z
discontinued: false
dominant: false
experience: experience/None
group u list: _37_
m user id: 0
owner id: 88
owning group id: 37
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
component: BBa_C0179
component: BBa_B0010
component: BBa_R0040
component: BBa_C0078
component: BBa_R0065
component: BBa_C0061
component: BBa_C0062
component: BBa_B0012
component: BBa_B0034
component: BBa_I14016
sequence annotation: BBa_B0034
sequence annotation: BBa_C0179
sequence annotation: BBa_B0012
sequence annotation: BBa_C0078
sequence annotation: BBa_R0065
sequence annotation: BBa_C0061
sequence annotation: BBa_B0010
sequence annotation: BBa_R0040
sequence annotation: BBa_I14016
sequence annotation: BBa_C0062
sequence: https://synbiohub.org/public/igem/BBa_J13031_sequence/1

BBa_C0179
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persistent identity: component1556431
display id: component1556431
version: 1
top level: BBa_J13031
access: public
definition: https://synbiohub.org/public/igem/BBa_C0179/1

BBa_B0010
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persistent identity: component1556396
display id: component1556396
version: 1
top level: BBa_J13031
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_R0040
type: Component
persistent identity: component1556421
display id: component1556421
version: 1
top level: BBa_J13031
access: public
definition: https://synbiohub.org/public/igem/BBa_R0040/1

BBa_C0078
type: Component
persistent identity: component1556390
display id: component1556390
version: 1
top level: BBa_J13031
access: public
definition: https://synbiohub.org/public/igem/BBa_C0078/1

BBa_R0065
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persistent identity: component1556263
display id: component1556263
version: 1
top level: BBa_J13031
access: public
definition: https://synbiohub.org/public/igem/BBa_R0065/1

BBa_C0061
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persistent identity: component1556285
display id: component1556285
version: 1
top level: BBa_J13031
access: public
definition: https://synbiohub.org/public/igem/BBa_C0061/1

BBa_C0062
type: Component
persistent identity: component1556340
display id: component1556340
version: 1
top level: BBa_J13031
access: public
definition: https://synbiohub.org/public/igem/BBa_C0062/1

BBa_B0012
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persistent identity: component1556406
display id: component1556406
version: 1
top level: BBa_J13031
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0010
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persistent identity: component1556292
display id: component1556292
version: 1
top level: BBa_J13031
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0010
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persistent identity: component1556348
display id: component1556348
version: 1
top level: BBa_J13031
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0034
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persistent identity: component1556429
display id: component1556429
version: 1
top level: BBa_J13031
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0012
type: Component
persistent identity: component1556446
display id: component1556446
version: 1
top level: BBa_J13031
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_R0040
type: Component
persistent identity: component1556317
display id: component1556317
version: 1
top level: BBa_J13031
access: public
definition: https://synbiohub.org/public/igem/BBa_R0040/1

BBa_B0012
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persistent identity: component1556358
display id: component1556358
version: 1
top level: BBa_J13031
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0034
type: Component
persistent identity: component1556380
display id: component1556380
version: 1
top level: BBa_J13031
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0012
type: Component
persistent identity: component1556302
display id: component1556302
version: 1
top level: BBa_J13031
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0034
type: Component
persistent identity: component1556275
display id: component1556275
version: 1
top level: BBa_J13031
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0010
type: Component
persistent identity: component1556436
display id: component1556436
version: 1
top level: BBa_J13031
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_I14016
type: Component
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BBa_I0500
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BBa_B0010
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BBa_B0034
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BBa_I13023
type: ComponentDefinition
persistent identity: BBa I13023
display id: BBa_I13023
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: QPI Test Construct Intermediate (Q01400.E0430)
creator: Barry Canton
created: 2004-07-13T11:00:00Z
modified: 2015-08-31T04:07:32Z
discontinued: false
dominant: false
experience: experience/None
group u list: _6_
m user id: 0
owner id: 159
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
component: BBa_B0010
component: BBa_B0031
component: BBa_B0034
component: BBa_E0030
component: BBa_C0040
component: BBa_R0040
component: BBa_B0012
sequence annotation: BBa_R0040
sequence annotation: BBa_B0010
sequence annotation: BBa_C0040
sequence annotation: BBa_E0030
sequence annotation: BBa_B0012
sequence annotation: BBa_B0031
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_I13023_sequence/1

BBa_B0010
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persistent identity: component962652
display id: component962652
version: 1
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access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0031
type: Component
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display id: component962636
version: 1
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access: public
definition: https://synbiohub.org/public/igem/BBa_B0031/1

BBa_B0010
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definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0034
type: Component
persistent identity: component962685
display id: component962685
version: 1
top level: BBa_I13023
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_E0030
type: Component
persistent identity: component962687
display id: component962687
version: 1
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definition: https://synbiohub.org/public/igem/BBa_E0030/1

BBa_C0040
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definition: https://synbiohub.org/public/igem/BBa_C0040/1

BBa_R0040
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definition: https://synbiohub.org/public/igem/BBa_R0040/1

BBa_B0012
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access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0012
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display id: component962702
version: 1
top level: BBa_I13023
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_R0040
type: SequenceAnnotation
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BBa_C0040
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BBa_E0030
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BBa_B0012
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top level: BBa_I13023
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component: BBa_B0012

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persistent identity: range962702
display id: range962702
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direction: direction/forward
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start: 1750
end: 1790
orientation: inline

BBa_B0031
type: SequenceAnnotation
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display id: annotation962636
version: 1
top level: BBa_I13023
location: https://synbiohub.org/public/igem/BBa_I13023/annotation962636/range962636/1
component: BBa_B0031

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type: Range
persistent identity: range962636
display id: range962636
version: 1
direction: direction/forward
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start: 1
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orientation: inline

BBa_B0012
type: SequenceAnnotation
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display id: annotation962662
version: 1
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location: https://synbiohub.org/public/igem/BBa_I13023/annotation962662/range962662/1
component: BBa_B0012

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persistent identity: range962662
display id: range962662
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display id: annotation962685
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component: BBa_B0034

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persistent identity: range962685
display id: range962685
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direction: direction/forward
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start: 913
end: 924
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation962692
display id: annotation962692
version: 1
top level: BBa_I13023
location: https://synbiohub.org/public/igem/BBa_I13023/annotation962692/range962692/1
component: BBa_B0010

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type: Range
persistent identity: range962692
display id: range962692
version: 1
direction: direction/forward
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start: 1662
end: 1741
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BBa_I3102
type: ComponentDefinition
persistent identity: BBa I3102
display id: BBa_I3102
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: AraC trigger device (R1075.I3700)
creator: Stephen Lee, Roshan Kumar, Joe Levine, Ziyan Chu (Polkadorks, IAP 2004)
created: 2004-01-28T12:00:00Z
modified: 2015-08-31T04:07:41Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Arabinose-inducible trigger using araC. The trigger consists of a constitutive promoter controlling an araC buffer.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
component: BBa_B0012
component: BBa_R1075
component: BBa_B0010
component: BBa_C0080
component: BBa_R0080
component: BBa_B0034
sequence annotation: BBa_B0034
sequence annotation: BBa_R0080
sequence annotation: BBa_B0012
sequence annotation: BBa_B0010
sequence annotation: BBa_C0080
sequence annotation: BBa_R1075
sequence: https://synbiohub.org/public/igem/BBa_I3102_sequence/1

BBa_B0012
type: Component
persistent identity: component955877
display id: component955877
version: 1
top level: BBa_I3102
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_R1075
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persistent identity: component955846
display id: component955846
version: 1
top level: BBa_I3102
access: public
definition: https://synbiohub.org/public/igem/BBa_R1075/1

BBa_B0010
type: Component
persistent identity: component955867
display id: component955867
version: 1
top level: BBa_I3102
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_C0080
type: Component
persistent identity: component955861
display id: component955861
version: 1
top level: BBa_I3102
access: public
definition: https://synbiohub.org/public/igem/BBa_C0080/1

BBa_R0080
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persistent identity: component955900
display id: component955900
version: 1
top level: BBa_I3102
access: public
definition: https://synbiohub.org/public/igem/BBa_R0080/1

BBa_B0034
type: Component
persistent identity: component955851
display id: component955851
version: 1
top level: BBa_I3102
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation955851
display id: annotation955851
version: 1
top level: BBa_I3102
location: https://synbiohub.org/public/igem/BBa_I3102/annotation955851/range955851/1
component: BBa_B0034

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type: Range
persistent identity: range955851
display id: range955851
version: 1
direction: direction/forward
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start: 58
end: 69
orientation: inline

BBa_R0080
type: SequenceAnnotation
persistent identity: annotation955900
display id: annotation955900
version: 1
top level: BBa_I3102
location: https://synbiohub.org/public/igem/BBa_I3102/annotation955900/range955900/1
component: BBa_R0080

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persistent identity: range955900
display id: range955900
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direction: direction/forward
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start: 1161
end: 1309
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display id: annotation955877
version: 1
top level: BBa_I3102
location: https://synbiohub.org/public/igem/BBa_I3102/annotation955877/range955877/1
component: BBa_B0012

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persistent identity: range955877
display id: range955877
version: 1
direction: direction/forward
top level: BBa_I3102
start: 1112
end: 1152
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation955867
display id: annotation955867
version: 1
top level: BBa_I3102
location: https://synbiohub.org/public/igem/BBa_I3102/annotation955867/range955867/1
component: BBa_B0010

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type: Range
persistent identity: range955867
display id: range955867
version: 1
direction: direction/forward
top level: BBa_I3102
start: 1024
end: 1103
orientation: inline

BBa_C0080
type: SequenceAnnotation
persistent identity: annotation955861
display id: annotation955861
version: 1
top level: BBa_I3102
location: https://synbiohub.org/public/igem/BBa_I3102/annotation955861/range955861/1
component: BBa_C0080

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type: Range
persistent identity: range955861
display id: range955861
version: 1
direction: direction/forward
top level: BBa_I3102
start: 76
end: 990
orientation: inline

BBa_R1075
type: SequenceAnnotation
persistent identity: annotation955846
display id: annotation955846
version: 1
top level: BBa_I3102
location: https://synbiohub.org/public/igem/BBa_I3102/annotation955846/range955846/1
component: BBa_R1075

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type: Range
persistent identity: range955846
display id: range955846
version: 1
direction: direction/forward
top level: BBa_I3102
start: 1
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orientation: inline

BBa_I12060
type: ComponentDefinition
persistent identity: BBa I12060
display id: BBa_I12060
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: BL oscillator (I12040 based) with IPTG kickstart
creator: endelman
created: 2004-09-02T11:00:00Z
modified: 2015-08-31T04:07:31Z
discontinued: false
dominant: false
experience: experience/None
group u list: _3_
m user id: 0
owner id: 145
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Modified BL oscillator based on I12040. It can be "reset" by adding IPTG which stimulates production of the A protein (lambda cI) if used in LacIq strain. The B protein is 434 cI. The YFP-AAV reporter tracks the dynamics of the A protein.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
component: BBa_I12057
component: BBa_I12054
component: BBa_I12024
component: BBa_E0434
sequence annotation: BBa_I12024
sequence annotation: BBa_E0434
sequence annotation: BBa_I12057
sequence annotation: BBa_I12054
sequence: https://synbiohub.org/public/igem/BBa_I12060_sequence/1

BBa_I12057
type: Component
persistent identity: component2237808
display id: component2237808
version: 1
top level: BBa_I12060
access: public
definition: https://synbiohub.org/public/igem/BBa_I12057/1

BBa_I12054
type: Component
persistent identity: component2237779
display id: component2237779
version: 1
top level: BBa_I12060
access: public
definition: https://synbiohub.org/public/igem/BBa_I12054/1

BBa_I12024
type: Component
persistent identity: component2237761
display id: component2237761
version: 1
top level: BBa_I12060
access: public
definition: https://synbiohub.org/public/igem/BBa_I12024/1

BBa_E0434
type: Component
persistent identity: component2237818
display id: component2237818
version: 1
top level: BBa_I12060
access: public
definition: https://synbiohub.org/public/igem/BBa_E0434/1

BBa_I12024
type: SequenceAnnotation
persistent identity: annotation2237761
display id: annotation2237761
version: 1
top level: BBa_I12060
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component: BBa_I12024

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display id: range2237761
version: 1
direction: direction/reverse
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start: 1
end: 993
orientation: inline

BBa_E0434
type: SequenceAnnotation
persistent identity: annotation2237818
display id: annotation2237818
version: 1
top level: BBa_I12060
location: https://synbiohub.org/public/igem/BBa_I12060/annotation2237818/range2237818/1
component: BBa_E0434

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type: Range
persistent identity: range2237818
display id: range2237818
version: 1
direction: direction/reverse
top level: BBa_I12060
start: 3027
end: 3943
orientation: inline

BBa_I12057
type: SequenceAnnotation
persistent identity: annotation2237808
display id: annotation2237808
version: 1
top level: BBa_I12060
location: https://synbiohub.org/public/igem/BBa_I12060/annotation2237808/range2237808/1
component: BBa_I12057

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type: Range
persistent identity: range2237808
display id: range2237808
version: 1
direction: direction/reverse
top level: BBa_I12060
start: 1891
end: 3018
orientation: inline

BBa_I12054
type: SequenceAnnotation
persistent identity: annotation2237779
display id: annotation2237779
version: 1
top level: BBa_I12060
location: https://synbiohub.org/public/igem/BBa_I12060/annotation2237779/range2237779/1
component: BBa_I12054

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type: Range
persistent identity: range2237779
display id: range2237779
version: 1
direction: direction/reverse
top level: BBa_I12060
start: 1002
end: 1882
orientation: inline

BBa_I15030
type: ComponentDefinition
persistent identity: BBa I15030
display id: BBa_I15030
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: 3OC6HSL amplifier
creator: Jeff Tabor
created: 2005-05-01T11:00:00Z
modified: 2015-08-31T04:07:39Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _37_5_
m user id: 0
owner id: 88
owning group id: 5
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This device is a strong amplifier of 3OC6HSL. R0065 is a leaky promoter that produces enough LuxI to generate ample HSL to switch this device to a stable high expression state, the magnitude of which is dependent upon LuxR (or possibly lambda cI repressor) concentration.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
component: BBa_B0012
component: BBa_C0061
component: BBa_B0010
component: BBa_B0034
component: BBa_C0062
component: BBa_R0040
component: BBa_R0065
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_C0062
sequence annotation: https://synbiohub.org/public/igem/BBa_I15030/annotation1482766/1
sequence annotation: https://synbiohub.org/public/igem/BBa_I15030/annotation1482756/1
sequence annotation: https://synbiohub.org/public/igem/BBa_I15030/annotation1482806/1
sequence annotation: https://synbiohub.org/public/igem/BBa_I15030/annotation1482839/1
sequence annotation: https://synbiohub.org/public/igem/BBa_I15030/annotation1482744/1
sequence annotation: https://synbiohub.org/public/igem/BBa_I15030/annotation1482773/1
sequence annotation: https://synbiohub.org/public/igem/BBa_I15030/annotation1482798/1
sequence: https://synbiohub.org/public/igem/BBa_I15030_sequence/1

BBa_B0012
type: Component
persistent identity: component1482839
display id: component1482839
version: 1
top level: BBa_I15030
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_C0061
type: Component
persistent identity: component1482766
display id: component1482766
version: 1
top level: BBa_I15030
access: public
definition: https://synbiohub.org/public/igem/BBa_C0061/1

BBa_B0010
type: Component
persistent identity: component1482829
display id: component1482829
version: 1
top level: BBa_I15030
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0034
type: Component
persistent identity: component1482806
display id: component1482806
version: 1
top level: BBa_I15030
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_C0062
type: Component
persistent identity: component1482821
display id: component1482821
version: 1
top level: BBa_I15030
access: public
definition: https://synbiohub.org/public/igem/BBa_C0062/1

BBa_B0010
type: Component
persistent identity: component1482773
display id: component1482773
version: 1
top level: BBa_I15030
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_R0040
type: Component
persistent identity: component1482798
display id: component1482798
version: 1
top level: BBa_I15030
access: public
definition: https://synbiohub.org/public/igem/BBa_R0040/1

BBa_B0012
type: Component
persistent identity: component1482783
display id: component1482783
version: 1
top level: BBa_I15030
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0034
type: Component
persistent identity: component1482756
display id: component1482756
version: 1
top level: BBa_I15030
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_R0065
type: Component
persistent identity: component1482744
display id: component1482744
version: 1
top level: BBa_I15030
access: public
definition: https://synbiohub.org/public/igem/BBa_R0065/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1482829
display id: annotation1482829
version: 1
top level: BBa_I15030
location: https://synbiohub.org/public/igem/BBa_I15030/annotation1482829/range1482829/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I15030/annotation1482829/range1482829/1
type: Range
persistent identity: range1482829
display id: range1482829
version: 1
direction: direction/forward
top level: BBa_I15030
start: 1781
end: 1860
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1482783
display id: annotation1482783
version: 1
top level: BBa_I15030
location: https://synbiohub.org/public/igem/BBa_I15030/annotation1482783/range1482783/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I15030/annotation1482783/range1482783/1
type: Range
persistent identity: range1482783
display id: range1482783
version: 1
direction: direction/forward
top level: BBa_I15030
start: 863
end: 903
orientation: inline

BBa_C0062
type: SequenceAnnotation
persistent identity: annotation1482821
display id: annotation1482821
version: 1
top level: BBa_I15030
location: https://synbiohub.org/public/igem/BBa_I15030/annotation1482821/range1482821/1
component: BBa_C0062

https://synbiohub.org/public/igem/BBa_I15030/annotation1482821/range1482821/1
type: Range
persistent identity: range1482821
display id: range1482821
version: 1
direction: direction/forward
top level: BBa_I15030
start: 992
end: 1747
orientation: inline