The repression of heat shock gene expression (ROSE) element is an RNA element found in the 5' UTR of some heat shock protein's mRNAs. The ROSE element is an RNA thermometer that negatively regulates heat shock gene expression. The secondary structure is thought to be altered by temperature, thus it is an RNA thermometer. This structure blocks access to the ribosome binding site at normal temperatures. During heat shock however, the structure changes freeing the ribosome binding site and allowing expression to occur. RNA Thermometers are the only known single-component regulators of gene expression.(1) RNA Thermometers are thermosensors that regulate gene expression by temperature-induced changes in RNA conformation. Naturally occurring RNA Thermometers exhibit complex secondary structures which are believed to undergo a series of gradual structural changes in response to temperature shifts.(2) RNA Thermometers often regulate genes required during either a heat shock or cold shock response, but have been implicated in other regulatory roles such as in pathogenecity and starvation.(3)
Note that RNA thermometer actually works as an RBS sequence. Therefore, this part does not include an additional ribosome binding site. Also, this part does not contain an terminator sequence, but a simple stop codon. The RFP should be translated continuously.
Bradyrhizobium japonicum USDA 110 and Registry
Repression of heat shock gene expression (ROSE) element The repression of heat shock gene expression (ROSE) element is an RNA element found in the 5' UTR of some heat shock protein's mRNAs. The ROSE element is an RNA thermometer that negatively regulates heat shock gene expression. The secondary structure is thought to be altered by temperature, thus it is an RNA thermometer. This structure blocks access to the ribosome binding site at normal temperatures. During heat shock however, the structure changes freeing the ribosome binding site and allowing expression to occur.[2][3] A partial structure of this RNA element has been determined using NMR.[1] ROSE1 and ROSEAT2 are specific examples of ROSE elements.[4] ROSE1 is found in Bradyrhizobium japonicum whereas ROSEAT2 is a closely related element from Agrobacterium tumefaciens. The two RNA elements have similar secondary structures with ROSE1 having an extra hairpin.[4] All ROSE elements contain a characteristic 'bulged G' opposite the Shine-Dalgarno sequence binding site, without this nucleotide the RNA thermometer loses its temperature-sensitivity.[5]
Jay Blumling, Debra Lin, Madeleine Sheldon-dante, Fred Tan, MIT SMUG
Constitutive (tetR repressible) LacI and RFP expression
This part constitutively expresses LacI and RFP except when repressed by TetR. When cells containing this part are exposed to the inducer molecule IPTG, it can be used to regulate lac-repressible promoters. It was designed to function as an IPTG-controllable tuner for the [http://partsregistry.org/Part:BBa_K546546 Double Tunable Synchronized Oscillator]. It is advisable to operate this part on a low or medium copy plasmid.
Contains IPTG inducible promoter, an RBS, GFP (no LVA tag), and a terminator.
The part include a RNAT, which is a temperature-sensing sequence, in front of the reporter eGFP. As the environmental temperature changes below 30℃, the RNAT folds into a series of different secondary structures and block ribosomes??? access to the mRNA thus hinder translation of the eGFP.
The SD sequence that benefits the bond between the RNA and the ribosome is designed in front of the eGFP sequence.
The RNAT sequence is designed by us, using computational method. The eGFP gene is from the iGEM part,BBa_I714891.
This is a firefly luciferase gene under the control of a strong constitutive promoter.
strong promoter, strong RBS, used this to demonstrate that our luciferase BioBrick is working
* promoter and RBS from partsregistry * luciferase from pGL4.10luc2 (Promega)
High expressed Alkane Selector for intracellular alkane production
medium strong constitutive promoter + luc
This is a firefly luciferase gene under the control of a medium strong constitutive promoter.
medium strong promoter, strong RBS, used this to demonstrate that our luciferase BioBrick is working
This circuit is composed of the constitutive promoter, ho1 chromoprotein and pcya chormoprotein. They facilitate Membrane protein ccaS and cph8 to be activated by light of certain wavelength.
Protein ho1 and protein pcyA have to combine with each other to function, so we put them behind the same promoter.
This part is from paper "Refactoring and Optimization of Light-Switchable Escherichia coli Two-Component Systems", and obtained from iGEM team HZAU's lab
Yellow Fluorescent Protein coding sequence expressed under a constitutive promoter for use as positive control in fluorescence measurement experiments.
Listed BioBrick parts from the Registry.
A composite part between J23100 and sfGFP
This part is an RFP coding device. When ligated with a promoter, it will yield a red colony so that you can determine whether your promoter works.
This is a firefly luciferase gene under the control of a weak constitutive promoter.
weak promoter, strong RBS, used this to demonstrate that our luciferase BioBrick is working
The IbpB thermometer is an RNA thermometer element found in the ibpAB operon. The operon contains two heat-shock genes, encoding inclusion body binding proteins A and B (IbpA/B), and is the most drastically upregulated operon under heat-shock in Escherichia coli. IbpA is regulated by a ROSE element found in its 5' UTR, while IbpB has its own heat-sensitive cis-regulatory element. The activity of this thermoregulator was confirmed in vitro but was not found in vivo, suggesting more complicated operon regulation exists in bacterial cells. In this part, red fluorescent protein (RFP) is added in the downstream region to measure the activity of RNA thermometer. You check our Experience page for further information about our characterization and experiments. For the inner elements in the part design; check out the Part Design page. RNA Thermometers are the only known single-component regulators of gene expression.(1) RNA Thermometers are thermosensors that regulate gene expression by temperature-induced changes in RNA conformation. Naturally occurring RNA Thermometers exhibit complex secondary structures which are believed to undergo a series of gradual structural changes in response to temperature shifts.(2) RNA Thermometers often regulate genes required during either a heat shock or cold shock response, but have been implicated in other regulatory roles such as in pathogenecity and starvation.(3)
Escherichia coli, NCBI GeneBank Other elements are obtained from Registry.