mCherry, bacterial with RBS and forward terminator
Combines BBa_B0015 forward terminator with BBa_J06602 mCherry, bacterial with RBS
This part was designed to incorporate CFP with an LVA degradation tag.
This part was constructed from registry biobricks.
Jay Blumling, Debra Lin, Madeleine Sheldon-dante, Fred Tan, MIT SMUG
Venus YFP with RBS and forward terminator
Combines BBa_B0034 RBS (Elowitz 1999) with BBa_J06600 Venus YFP with forward terminator
Test constructs involving this part had no fluorescence observed. Sequencing returned nothing resembling the expected part.
This part contains a green fluorescence protein (GFP) under control of a CMV promoter and its double terminator (BBa_I712669 + BBa_B0014). This part is reliable to use when we want to know if a gene is inserted into a eukaryotic cell. For example we can use it when we design a recombinant virus. In fact we inserted this bio-brick into the synthetic virus; finally we are able to see if the virus can infect the targeted cells. This bio-brick already contains a terminator, so it is ready to use.
Part:BBa_I712669, Designed by Rok Gaber Group: iGEM07_Ljubljana (2007-10-24) Part:BBa_B0014, Designed by Reshma Shetty Group: Registry (2003-07-16)
BBa_R0010 Promoter (RBS+) with BBa_J04671 (EYFP, RBS-, LVA+, B0015)
This is a construct with the Promoter Sequence BBa_R0010 and the Reporter Construct BBa_J04671 (EYFP, RBS-, LVA+, B0015)
This part was constructed from parts available from the registry.
rbs-GFP(LVA)-terminators (Backbone: pSB1A2)
This part is a generator that lacks a promoter. The GFP has a LVA degradation tail. Upon expression, the GFP will give green fluorescence color.
mCherry, bacterial (LVA+) with RBS and forward terminator
Combines BBa_B0015 forward terminator with BBa_J06603 mCherry, bacterial (LVA+) with RBS
B0034.E0034.B0015 output device with aav tagged yfp
medium RBS, YFP (-LVA), double terminator
This part was designed to include an AAV degradation tag.
B0034.E0036.B0015 output device with asv tagged yfp
Green fluorescent protein (E0240) is constitutively produced thanks to a constitutive promoter (J23119).
This part was build following the standard construction protocol.
J23119 is an E.coli constitutive promoter. The GFP sequence included in E0240 is a green fluorescent protein derived from jellyfish Aequeora victoria wild type GFP.
Combines BBa_B0034 RBS (Elowitz 1999) with BBa_J06601 mCherry with forward terminator
Threshold: Reporter module (regulated by cI)
Part of the thresholding device (works with j07036, j07038/j07039/j07040). This encodes the reporter gene that is regulated by the cI repressor (coded by j07036). The amount of cI itself is regulated by j07038/39/40 that code for antisense mRNA to the cI transcript, and hence repress translation of cI (and activate j07037)
Built by Josh as an intermediate in screening plasmid construction.