//classic/rna/uncategorized
type: Collection
persistent identity: classic rna uncategorized
display id: classic_rna_uncategorized
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
owned by: james
owned by: myers
member: BBa_I1041
member: Key 2
member: BBa_I1033
member: [pTet][key
member: BBa_J04805
member: BBa_I1012
member: BBa_I1032
member: BBa_I1034
member: Lock 2
member: BBa_I1040
member: BBa_I1013
member: BBa_I1031
member: BBa_J04686
member: BBa_I1022
member: BBa_I1060
member: BBa_I13536
member: BBa_I1030
member: BBa_J04605
member: BBa_I1044
member: BBa_J04685
member: BBa_I1023
member: BBa_I1021
member: BBa_J04676
member: BBa_I1042
member: BBa_J04086
member: BBa_J04085
member: BBa_I1063
member: BBa_I1011
member: BBa_I1020
member: Key 1

Key 2
type: ComponentDefinition
persistent identity: BBa J01009
display id: BBa_J01009
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Riboregulator Key 2
creator: Golden Bear
created: 2005-11-05T12:00:00Z
modified: 2015-08-31T04:08:12Z
discontinued: true
dominant: false
group u list: _13_
m user id: 0
owner id: 395
owning group id: 13
sample status: Discontinued
status: status/Deleted
bookmark: false
mutable description: Another variant of biobricked version of Isaacs' riboregulator trans activating key, taR12, only change is in the address region.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000834
role: partType/RNA
sequence: https://synbiohub.org/public/igem/BBa_J01009_sequence/1

BBa_I1032
type: ComponentDefinition
persistent identity: BBa I1032
display id: BBa_I1032
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CI(1) "micRNA" asRNA
creator: Grace Kenney, Daniel Shen, Neelaksh Varshney, Samantha Sutton
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:29Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Region which serves as basis for transcription of asRNA that binds to and inhibits <bb_part>BBa_I1030</bb_part>'s mRNA transcript. Has LacO-1 regulatory region and a Promoter. Part of the XOR gate comprised of <bb_part>BBa_I1030</bb_part> and <bb_part>BBa_I1040</bb_part>, their corresponding asRNA coding sequences (<bb_part>BBa_I1012</bb_part> and <bb_part>BBa_I1032</bb_part>).
mutable notes: Complementary to beginning of <bb_part>BBa_I1030</bb_part> transcript covering junk region, RBS, start codon, and 73 bp into coding sequence. Two stem loops flank the antisense region. [KEIL96] Incompatible with systems containing <bb_part>BBa_I1031</bb_part> Compatible with <bb_part>BBa_I1020</bb_part>, <bb_part>BBa_I1021</bb_part>, <bb_part>BBa_I1022</bb_part>, <bb_part>BBa_I1023</bb_part>.
mutable provenance: see references
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000834
role: partType/RNA
sequence annotation: LacO-1
sequence annotation: BBa_I1032
sequence annotation: antisense region for I1030
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_I1032_sequence/1

LacO-1
type: SequenceAnnotation
persistent identity: annotation1891
display id: annotation1891
version: 1
top level: BBa_I1032
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_I1032/annotation1891/range1891/1

https://synbiohub.org/public/igem/BBa_I1032/annotation1891/range1891/1
type: Range
persistent identity: range1891
display id: range1891
version: 1
direction: direction/forward
top level: BBa_I1032
start: 1
end: 55
orientation: inline

BBa_I1032
type: SequenceAnnotation
persistent identity: annotation7052
display id: annotation7052
version: 1
top level: BBa_I1032
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_I1032/annotation7052/range7052/1

https://synbiohub.org/public/igem/BBa_I1032/annotation7052/range7052/1
type: Range
persistent identity: range7052
display id: range7052
version: 1
direction: direction/forward
top level: BBa_I1032
start: 1
end: 255
orientation: inline

antisense region for I1030
type: SequenceAnnotation
persistent identity: annotation1888
display id: annotation1888
version: 1
top level: BBa_I1032
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I1032/annotation1888/range1888/1

https://synbiohub.org/public/igem/BBa_I1032/annotation1888/range1888/1
type: Range
persistent identity: range1888
display id: range1888
version: 1
direction: direction/forward
top level: BBa_I1032
start: 113
end: 220
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation1889
display id: annotation1889
version: 1
top level: BBa_I1032
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_I1032/annotation1889/range1889/1

https://synbiohub.org/public/igem/BBa_I1032/annotation1889/range1889/1
type: Range
persistent identity: range1889
display id: range1889
version: 1
direction: direction/forward
top level: BBa_I1032
start: 56
end: 112
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation1890
display id: annotation1890
version: 1
top level: BBa_I1032
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_I1032/annotation1890/range1890/1

https://synbiohub.org/public/igem/BBa_I1032/annotation1890/range1890/1
type: Range
persistent identity: range1890
display id: range1890
version: 1
direction: direction/forward
top level: BBa_I1032
start: 221
end: 255
orientation: inline

BBa_I1020
type: ComponentDefinition
persistent identity: BBa I1020
display id: BBa_I1020
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Custom cI(2) Protein w/LacO-1 Promoter
creator: Samantha Sutton, Neelaksh Varshney, Daniel Shen, Grace Kenney
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:29Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Coding sequence for modified cI protein with LacO-1 promoter for antisense IS10 mRNA regulation.
mutable notes: <bb_part>BBa_I1020</bb_part> Custom cI Protein with LacO-1 Promoter is based on BioBricks <bb_part>BBa_C0050</bb_part> and <bb_part>BBa_R0011</bb_part>. It is designed such that <bb_part>BBa_I1023</bb_part> can be used to interfere with the <bb_part>BBa_1020</bb_part> transcript by anti-sense mRNA binding, in the IS10 method shown below. <bb_part>BBa_I1020</bb_part> and <bb_part>BBa_I1023</bb_part> can act indepently of related biobrick parts <bb_part>BBa_1010</bb_part>, <bb_part>BBa_1011</bb_part>, <bb_part>BBa_1012</bb_part>, <bb_part>BBa_I1030</bb_part>, <bb_part>BBa_I1031</bb_part>, <bb_part>BBa_I1032</bb_part>, <bb_part>BBa_I1041</bb_part>, <bb_part>BBa_I1042</bb_part>, <bb_part>BBa_I1060</bb_part>, <bb_part>BBa_I1063</bb_part> and <bb_part>BBa_1013</bb_part>. Can be used with all parts of the BBa_I system. It is inhibited by BBa_I1023. Incompatible with systems containing cI (wild type)
mutable provenance: Modified from BioBricks <bb_part>BBa_R0011</bb_part>, and <bb_part>BBa_C0051</bb_part>.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000834
role: partType/RNA
sequence annotation: BBa_I1020
sequence annotation: -10
sequence annotation: cI lambda
sequence annotation: Homology to I1023
sequence annotation: C added
sequence annotation: rbs
sequence annotation: -35
sequence annotation: start
sequence annotation: LacO-1
sequence annotation: 2
sequence annotation: HH117a mutation specific to I1020 and I1023
sequence annotation: Complementary to Antisense mRNA I1023
sequence annotation: LacO-1 region
sequence annotation: SsrA
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_I1020_sequence/1

BBa_I1020
type: SequenceAnnotation
persistent identity: annotation7046
display id: annotation7046
version: 1
top level: BBa_I1020
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_I1020/annotation7046/range7046/1

https://synbiohub.org/public/igem/BBa_I1020/annotation7046/range7046/1
type: Range
persistent identity: range7046
display id: range7046
version: 1
direction: direction/forward
top level: BBa_I1020
start: 1
end: 835
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1826
display id: annotation1826
version: 1
top level: BBa_I1020
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I1020/annotation1826/range1826/1

https://synbiohub.org/public/igem/BBa_I1020/annotation1826/range1826/1
type: Range
persistent identity: range1826
display id: range1826
version: 1
direction: direction/forward
top level: BBa_I1020
start: 44
end: 49
orientation: inline

cI lambda
type: SequenceAnnotation
persistent identity: annotation1824
display id: annotation1824
version: 1
top level: BBa_I1020
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_I1020/annotation1824/range1824/1

https://synbiohub.org/public/igem/BBa_I1020/annotation1824/range1824/1
type: Range
persistent identity: range1824
display id: range1824
version: 1
direction: direction/forward
top level: BBa_I1020
start: 83
end: 835
orientation: inline

Homology to I1023
type: SequenceAnnotation
persistent identity: annotation1835
display id: annotation1835
version: 1
top level: BBa_I1020
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I1020/annotation1835/range1835/1

https://synbiohub.org/public/igem/BBa_I1020/annotation1835/range1835/1
type: Range
persistent identity: range1835
display id: range1835
version: 1
direction: direction/forward
top level: BBa_I1020
start: 89
end: 90
orientation: inline

C added
type: SequenceAnnotation
persistent identity: annotation1834
display id: annotation1834
version: 1
top level: BBa_I1020
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_I1020/annotation1834/range1834/1

https://synbiohub.org/public/igem/BBa_I1020/annotation1834/range1834/1
type: Range
persistent identity: range1834
display id: range1834
version: 1
direction: direction/forward
top level: BBa_I1020
start: 86
end: 88
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation1821
display id: annotation1821
version: 1
top level: BBa_I1020
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_I1020/annotation1821/range1821/1

https://synbiohub.org/public/igem/BBa_I1020/annotation1821/range1821/1
type: Range
persistent identity: range1821
display id: range1821
version: 1
direction: direction/forward
top level: BBa_I1020
start: 73
end: 78
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1825
display id: annotation1825
version: 1
top level: BBa_I1020
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I1020/annotation1825/range1825/1

https://synbiohub.org/public/igem/BBa_I1020/annotation1825/range1825/1
type: Range
persistent identity: range1825
display id: range1825
version: 1
direction: direction/forward
top level: BBa_I1020
start: 21
end: 26
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation1823
display id: annotation1823
version: 1
top level: BBa_I1020
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_I1020/annotation1823/range1823/1

https://synbiohub.org/public/igem/BBa_I1020/annotation1823/range1823/1
type: Range
persistent identity: range1823
display id: range1823
version: 1
direction: direction/forward
top level: BBa_I1020
start: 83
end: 85
orientation: inline

LacO-1
type: SequenceAnnotation
persistent identity: annotation1831
display id: annotation1831
version: 1
top level: BBa_I1020
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I1020/annotation1831/range1831/1

https://synbiohub.org/public/igem/BBa_I1020/annotation1831/range1831/1
type: Range
persistent identity: range1831
display id: range1831
version: 1
direction: direction/forward
top level: BBa_I1020
start: 27
end: 43
orientation: inline

2
type: SequenceAnnotation
persistent identity: annotation1829
display id: annotation1829
version: 1
top level: BBa_I1020
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_I1020/annotation1829/range1829/1

https://synbiohub.org/public/igem/BBa_I1020/annotation1829/range1829/1
type: Range
persistent identity: range1829
display id: range1829
version: 1
direction: direction/forward
top level: BBa_I1020
start: 830
end: 835
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation1832
display id: annotation1832
version: 1
top level: BBa_I1020
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_I1020/annotation1832/range1832/1

https://synbiohub.org/public/igem/BBa_I1020/annotation1832/range1832/1
type: Range
persistent identity: range1832
display id: range1832
version: 1
direction: direction/forward
top level: BBa_I1020
start: 56
end: 56
orientation: inline

HH117a mutation specific to I1020 and I1023
type: SequenceAnnotation
persistent identity: annotation1833
display id: annotation1833
version: 1
top level: BBa_I1020
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_I1020/annotation1833/range1833/1

https://synbiohub.org/public/igem/BBa_I1020/annotation1833/range1833/1
type: Range
persistent identity: range1833
display id: range1833
version: 1
direction: direction/forward
top level: BBa_I1020
start: 58
end: 58
orientation: inline

Complementary to Antisense mRNA I1023
type: SequenceAnnotation
persistent identity: annotation1827
display id: annotation1827
version: 1
top level: BBa_I1020
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I1020/annotation1827/range1827/1

https://synbiohub.org/public/igem/BBa_I1020/annotation1827/range1827/1
type: Range
persistent identity: range1827
display id: range1827
version: 1
direction: direction/forward
top level: BBa_I1020
start: 56
end: 90
orientation: inline

LacO-1 region
type: SequenceAnnotation
persistent identity: annotation1830
display id: annotation1830
version: 1
top level: BBa_I1020
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I1020/annotation1830/range1830/1

https://synbiohub.org/public/igem/BBa_I1020/annotation1830/range1830/1
type: Range
persistent identity: range1830
display id: range1830
version: 1
direction: direction/forward
top level: BBa_I1020
start: 1
end: 20
orientation: inline

SsrA
type: SequenceAnnotation
persistent identity: annotation1828
display id: annotation1828
version: 1
top level: BBa_I1020
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_I1020/annotation1828/range1828/1

https://synbiohub.org/public/igem/BBa_I1020/annotation1828/range1828/1
type: Range
persistent identity: range1828
display id: range1828
version: 1
direction: direction/forward
top level: BBa_I1020
start: 797
end: 835
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation1820
display id: annotation1820
version: 1
top level: BBa_I1020
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_I1020/annotation1820/range1820/1

https://synbiohub.org/public/igem/BBa_I1020/annotation1820/range1820/1
type: Range
persistent identity: range1820
display id: range1820
version: 1
direction: direction/forward
top level: BBa_I1020
start: 56
end: 85
orientation: inline

BBa_J04605
type: ComponentDefinition
persistent identity: BBa J04605
display id: BBa_J04605
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: R0010 with Anti-Sense Theophillline Induced Antiswitch ("On Switch")
creator: Oscar Hernandez and Matt Gemberling
created: 2005-09-14T11:00:00Z
modified: 2015-08-31T04:08:14Z
discontinued: false
dominant: false
group u list: _16_
m user id: 0
owner id: 435
owning group id: 16
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This part contains the R0010 Promoter region attached to an anti-sense antiswitch sequence for the regulation of EYFP This Promoter and Antiswitch construct is designed so that it can be attached to any form of the EYFP coding region. The purpose of this piece is to regulate the expression of YFP in the presence of theophylline. In this construct, the presence of theophylline causes the antiswitch, which is initially bound to the YFP coding region (preventing expression), to release and fold up on itself allowing for EYFP expression.
mutable notes: Adaptation of antiswitch design "S8" by Bayer and Smolke; article available at http://www.nature.com/nbt/journal/v23/n3/full/nbt1069.html
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000834
role: partType/RNA
sequence annotation: R0010 Promoter Region
sequence annotation: Antisense Binding Sequence
sequence annotation: Antiswitch
sequence annotation: Hammerhead Riobozyme #2
sequence annotation: Hammerhead Riobozyme #1
sequence: https://synbiohub.org/public/igem/BBa_J04605_sequence/1

R0010 Promoter Region
type: SequenceAnnotation
persistent identity: annotation1691965
display id: annotation1691965
version: 1
top level: BBa_J04605
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_J04605/annotation1691965/range1691965/1

https://synbiohub.org/public/igem/BBa_J04605/annotation1691965/range1691965/1
type: Range
persistent identity: range1691965
display id: range1691965
version: 1
direction: direction/forward
top level: BBa_J04605
start: 1
end: 200
orientation: inline

Antisense Binding Sequence
type: SequenceAnnotation
persistent identity: annotation1691995
display id: annotation1691995
version: 1
top level: BBa_J04605
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_J04605/annotation1691995/range1691995/1

https://synbiohub.org/public/igem/BBa_J04605/annotation1691995/range1691995/1
type: Range
persistent identity: range1691995
display id: range1691995
version: 1
direction: direction/forward
top level: BBa_J04605
start: 254
end: 268
orientation: inline

Antiswitch
type: SequenceAnnotation
persistent identity: annotation1691967
display id: annotation1691967
version: 1
top level: BBa_J04605
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J04605/annotation1691967/range1691967/1

https://synbiohub.org/public/igem/BBa_J04605/annotation1691967/range1691967/1
type: Range
persistent identity: range1691967
display id: range1691967
version: 1
direction: direction/forward
top level: BBa_J04605
start: 254
end: 308
orientation: inline

Hammerhead Riobozyme #2
type: SequenceAnnotation
persistent identity: annotation1691971
display id: annotation1691971
version: 1
top level: BBa_J04605
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_J04605/annotation1691971/range1691971/1

https://synbiohub.org/public/igem/BBa_J04605/annotation1691971/range1691971/1
type: Range
persistent identity: range1691971
display id: range1691971
version: 1
direction: direction/forward
top level: BBa_J04605
start: 309
end: 359
orientation: inline

Hammerhead Riobozyme #1
type: SequenceAnnotation
persistent identity: annotation1691966
display id: annotation1691966
version: 1
top level: BBa_J04605
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_J04605/annotation1691966/range1691966/1

https://synbiohub.org/public/igem/BBa_J04605/annotation1691966/range1691966/1
type: Range
persistent identity: range1691966
display id: range1691966
version: 1
direction: direction/forward
top level: BBa_J04605
start: 201
end: 253
orientation: inline

BBa_I13536
type: ComponentDefinition
persistent identity: BBa I13536
display id: BBa_I13536
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: T7 RNAseIII site
creator: Josh Michener
created: 2005-07-28T11:00:00Z
modified: 2015-08-31T04:07:35Z
discontinued: false
dominant: false
group u list: _11_
m user id: 0
owner id: 253
owning group id: 6
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: R0.3 RNAseIII site from T7
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000834
role: partType/RNA
sequence: https://synbiohub.org/public/igem/BBa_I13536_sequence/1

BBa_I1034
type: ComponentDefinition
persistent identity: BBa I1034
display id: BBa_I1034
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CI(1) "KISS" asRNA without promoter
creator: Grace Kenney, Daniel Shen, Neelaksh Varshney, Samantha Sutton
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:29Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Region which serves as basis for transcription of asRNA that binds to and inhibits <bb_part>BBa_I1030</bb_part>'s mRNA transcript. Part of the XOR gate comprised of <bb_part>BBa_I1030</bb_part> and <bb_part>BBa_I1040</bb_part>, their corresponding asRNA coding sequences (<bb_part>BBa_I1011</bb_part> and <bb_part>BBa_I1031</bb_part>).
mutable notes: Complementary to beginning of <bb_part>BBa_I1030</bb_part> transcript covering junk region, RBS, start codon, and 73 bp into coding sequence. No designed secondary structure. [KEIL96] Incompatible with systems containing <bb_part>BBa_I1032</bb_part> Compatible with <bb_part>BBa_I1020</bb_part>, <bb_part>BBa_I1021</bb_part>, <bb_part>BBa_I1022</bb_part>, <bb_part>BBa_I1023</bb_part>.
mutable provenance: see references
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000834
role: partType/RNA
sequence annotation: BBa_I1034
sequence annotation: antisense region for I1030
sequence: https://synbiohub.org/public/igem/BBa_I1034_sequence/1

BBa_I1034
type: SequenceAnnotation
persistent identity: annotation7054
display id: annotation7054
version: 1
top level: BBa_I1034
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_I1034/annotation7054/range7054/1

https://synbiohub.org/public/igem/BBa_I1034/annotation7054/range7054/1
type: Range
persistent identity: range7054
display id: range7054
version: 1
direction: direction/forward
top level: BBa_I1034
start: 1
end: 108
orientation: inline

antisense region for I1030
type: SequenceAnnotation
persistent identity: annotation1905
display id: annotation1905
version: 1
top level: BBa_I1034
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I1034/annotation1905/range1905/1

https://synbiohub.org/public/igem/BBa_I1034/annotation1905/range1905/1
type: Range
persistent identity: range1905
display id: range1905
version: 1
direction: direction/forward
top level: BBa_I1034
start: 1
end: 108
orientation: inline

BBa_J04085
type: ComponentDefinition
persistent identity: BBa J04085
display id: BBa_J04085
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: "On" Antiswitch and target EYFP in the same plasmid
creator: Matt Gemberling
created: 2005-12-04T12:00:00Z
modified: 2015-08-31T04:08:13Z
discontinued: false
dominant: false
experience: experience/Issues
group u list: _16_
m user id: 0
owner id: 428
owning group id: 16
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This is an "On" antiswitch placed into the same plasmid as the target EYFP. Both have the same promoter. This will help to ensure a close ratio of Antiswitch to EYFP mRNA. This part was designed based on Smolke and Bayer (2005).
mutable provenance: Adaptation of antiswitch design "S8" by Bayer and Smolke; article available at http://www.nature.com/nbt/journal/v23/n3/full/nbt1069.html
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000834
role: partType/RNA
sequence annotation: R0010
sequence annotation: B0015
sequence annotation: Antiswitch
sequence annotation: Hammerhead Ribozyme
sequence annotation: I6060
sequence annotation: EYFP
sequence: https://synbiohub.org/public/igem/BBa_J04085_sequence/1

R0010
type: SequenceAnnotation
persistent identity: annotation1759576
display id: annotation1759576
version: 1
top level: BBa_J04085
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_J04085/annotation1759576/range1759576/1

https://synbiohub.org/public/igem/BBa_J04085/annotation1759576/range1759576/1
type: Range
persistent identity: range1759576
display id: range1759576
version: 1
direction: direction/forward
top level: BBa_J04085
start: 1
end: 200
orientation: inline

B0015
type: SequenceAnnotation
persistent identity: annotation1759580
display id: annotation1759580
version: 1
top level: BBa_J04085
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_J04085/annotation1759580/range1759580/1

https://synbiohub.org/public/igem/BBa_J04085/annotation1759580/range1759580/1
type: Range
persistent identity: range1759580
display id: range1759580
version: 1
direction: direction/forward
top level: BBa_J04085
start: 359
end: 487
orientation: inline

R0010
type: SequenceAnnotation
persistent identity: annotation1759582
display id: annotation1759582
version: 1
top level: BBa_J04085
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_J04085/annotation1759582/range1759582/1

https://synbiohub.org/public/igem/BBa_J04085/annotation1759582/range1759582/1
type: Range
persistent identity: range1759582
display id: range1759582
version: 1
direction: direction/forward
top level: BBa_J04085
start: 488
end: 687
orientation: inline

Antiswitch
type: SequenceAnnotation
persistent identity: annotation1759578
display id: annotation1759578
version: 1
top level: BBa_J04085
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J04085/annotation1759578/range1759578/1

https://synbiohub.org/public/igem/BBa_J04085/annotation1759578/range1759578/1
type: Range
persistent identity: range1759578
display id: range1759578
version: 1
direction: direction/forward
top level: BBa_J04085
start: 254
end: 308
orientation: inline

B0015
type: SequenceAnnotation
persistent identity: annotation1759609
display id: annotation1759609
version: 1
top level: BBa_J04085
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_J04085/annotation1759609/range1759609/1

https://synbiohub.org/public/igem/BBa_J04085/annotation1759609/range1759609/1
type: Range
persistent identity: range1759609
display id: range1759609
version: 1
direction: direction/forward
top level: BBa_J04085
start: 1451
end: 1612
orientation: inline

Hammerhead Ribozyme
type: SequenceAnnotation
persistent identity: annotation1759579
display id: annotation1759579
version: 1
top level: BBa_J04085
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J04085/annotation1759579/range1759579/1

https://synbiohub.org/public/igem/BBa_J04085/annotation1759579/range1759579/1
type: Range
persistent identity: range1759579
display id: range1759579
version: 1
direction: direction/forward
top level: BBa_J04085
start: 309
end: 358
orientation: inline

I6060
type: SequenceAnnotation
persistent identity: annotation1759581
display id: annotation1759581
version: 1
top level: BBa_J04085
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_J04085/annotation1759581/range1759581/1

https://synbiohub.org/public/igem/BBa_J04085/annotation1759581/range1759581/1
type: Range
persistent identity: range1759581
display id: range1759581
version: 1
direction: direction/forward
top level: BBa_J04085
start: 488
end: 1612
orientation: inline

Hammerhead Ribozyme
type: SequenceAnnotation
persistent identity: annotation1759577
display id: annotation1759577
version: 1
top level: BBa_J04085
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J04085/annotation1759577/range1759577/1

https://synbiohub.org/public/igem/BBa_J04085/annotation1759577/range1759577/1
type: Range
persistent identity: range1759577
display id: range1759577
version: 1
direction: direction/forward
top level: BBa_J04085
start: 201
end: 253
orientation: inline

EYFP
type: SequenceAnnotation
persistent identity: annotation1759608
display id: annotation1759608
version: 1
top level: BBa_J04085
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J04085/annotation1759608/range1759608/1

https://synbiohub.org/public/igem/BBa_J04085/annotation1759608/range1759608/1
type: Range
persistent identity: range1759608
display id: range1759608
version: 1
direction: direction/forward
top level: BBa_J04085
start: 688
end: 1450
orientation: inline

BBa_J04685
type: ComponentDefinition
persistent identity: BBa J04685
display id: BBa_J04685
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Antiswitch enabling production of EYFP ("On" switch)
creator: Nicholas Cain, Davidson College
created: 2005-06-27T11:00:00Z
modified: 2015-08-31T04:08:15Z
discontinued: false
dominant: false
experience: experience/Issues
group u list: _16_
m user id: 0
owner id: 331
owning group id: 16
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: The presence of theophylline allows translation of mRNA transcribed from Biobrick BBa_E0032. No theophylline present causes repression of the coding region of BBa_E0032.
mutable notes: Transcription of the antiswitch is repressed in the presence of tetR.
mutable provenance: Adaptation of antiswitch design "S8" by Bayer and Smolke; article available at http://www.nature.com/nbt/journal/v23/n3/full/nbt1069.html
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000834
role: partType/RNA
sequence annotation: Antisense Binding Sequence
sequence annotation: Hammerhead Riobozyme #2
sequence annotation: R0010 Promoter Region
sequence annotation: Antiswitch
sequence annotation: Hammerhead Riobozyme #1
sequence annotation: B0015 Terminator
sequence: https://synbiohub.org/public/igem/BBa_J04685_sequence/1

Antisense Binding Sequence
type: SequenceAnnotation
persistent identity: annotation1553282
display id: annotation1553282
version: 1
top level: BBa_J04685
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_J04685/annotation1553282/range1553282/1

https://synbiohub.org/public/igem/BBa_J04685/annotation1553282/range1553282/1
type: Range
persistent identity: range1553282
display id: range1553282
version: 1
direction: direction/forward
top level: BBa_J04685
start: 108
end: 122
orientation: inline

Hammerhead Riobozyme #2
type: SequenceAnnotation
persistent identity: annotation1553286
display id: annotation1553286
version: 1
top level: BBa_J04685
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_J04685/annotation1553286/range1553286/1

https://synbiohub.org/public/igem/BBa_J04685/annotation1553286/range1553286/1
type: Range
persistent identity: range1553286
display id: range1553286
version: 1
direction: direction/forward
top level: BBa_J04685
start: 163
end: 213
orientation: inline

R0010 Promoter Region
type: SequenceAnnotation
persistent identity: annotation1553270
display id: annotation1553270
version: 1
top level: BBa_J04685
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_J04685/annotation1553270/range1553270/1

https://synbiohub.org/public/igem/BBa_J04685/annotation1553270/range1553270/1
type: Range
persistent identity: range1553270
display id: range1553270
version: 1
direction: direction/forward
top level: BBa_J04685
start: 1
end: 54
orientation: inline

Antiswitch
type: SequenceAnnotation
persistent identity: annotation1553272
display id: annotation1553272
version: 1
top level: BBa_J04685
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J04685/annotation1553272/range1553272/1

https://synbiohub.org/public/igem/BBa_J04685/annotation1553272/range1553272/1
type: Range
persistent identity: range1553272
display id: range1553272
version: 1
direction: direction/forward
top level: BBa_J04685
start: 108
end: 162
orientation: inline

Hammerhead Riobozyme #1
type: SequenceAnnotation
persistent identity: annotation1553271
display id: annotation1553271
version: 1
top level: BBa_J04685
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_J04685/annotation1553271/range1553271/1

https://synbiohub.org/public/igem/BBa_J04685/annotation1553271/range1553271/1
type: Range
persistent identity: range1553271
display id: range1553271
version: 1
direction: direction/forward
top level: BBa_J04685
start: 55
end: 107
orientation: inline

B0015 Terminator
type: SequenceAnnotation
persistent identity: annotation1553290
display id: annotation1553290
version: 1
top level: BBa_J04685
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J04685/annotation1553290/range1553290/1

https://synbiohub.org/public/igem/BBa_J04685/annotation1553290/range1553290/1
type: Range
persistent identity: range1553290
display id: range1553290
version: 1
direction: direction/forward
top level: BBa_J04685
start: 214
end: 341
orientation: inline

Key 1
type: ComponentDefinition
persistent identity: BBa J01008
display id: BBa_J01008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Riboregulator key 1
creator: Golden Bear
created: 2005-11-05T12:00:00Z
modified: 2015-08-31T04:08:12Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _13_
m user id: 0
owner id: 395
owning group id: 13
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Biobricked version of Isaacs' riboregulator trans activating key, taR12
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000834
role: partType/RNA
sequence: https://synbiohub.org/public/igem/BBa_J01008_sequence/1

[pTet][key
type: ComponentDefinition
persistent identity: BBa J01129
display id: BBa_J01129
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: [pTet][key3]
creator: Golden Bear
created: 2006-01-12T12:00:00Z
modified: 2015-08-31T04:08:13Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _13_
m user id: 0
owner id: 395
owning group id: 13
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: [pTet][key3]
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000834
role: partType/RNA
component: BBa_R0040
component: BBa_J01086
sequence annotation: BBa_R0040
sequence annotation: BBa_J01086
sequence: https://synbiohub.org/public/igem/BBa_J01129_sequence/1

BBa_R0040
type: Component
persistent identity: component1785010
display id: component1785010
version: 1
top level: [pTet][key
access: public
definition: https://synbiohub.org/public/igem/BBa_R0040/1

BBa_J01086
type: Component
persistent identity: component1785015
display id: component1785015
version: 1
top level: [pTet][key
access: public
definition: https://synbiohub.org/public/igem/BBa_J01086/1

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1785010
display id: annotation1785010
version: 1
top level: [pTet][key
location: https://synbiohub.org/public/igem/BBa_J01129/annotation1785010/range1785010/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_J01129/annotation1785010/range1785010/1
type: Range
persistent identity: range1785010
display id: range1785010
version: 1
direction: direction/forward
top level: [pTet][key
start: 1
end: 54
orientation: inline

BBa_J01086
type: SequenceAnnotation
persistent identity: annotation1785015
display id: annotation1785015
version: 1
top level: [pTet][key
location: https://synbiohub.org/public/igem/BBa_J01129/annotation1785015/range1785015/1
component: BBa_J01086

https://synbiohub.org/public/igem/BBa_J01129/annotation1785015/range1785015/1
type: Range
persistent identity: range1785015
display id: range1785015
version: 1
direction: direction/reverse
top level: [pTet][key
start: 63
end: 156
orientation: inline

BBa_J04086
type: ComponentDefinition
persistent identity: BBa J04086
display id: BBa_J04086
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: "Off" Antiswitch and target EYFP in the same plasmid
creator: Matt Gemberling
created: 2005-12-04T12:00:00Z
modified: 2015-08-31T04:08:13Z
discontinued: false
dominant: false
group u list: _16_
m user id: 0
owner id: 428
owning group id: 16
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This is an "Off" antiswitch placed into the same plasmid as the target EYFP. Both have the same promoter. This will help to ensure a close ratio of Antiswitch to EYFP mRNA. This part was designed based on Smolke and Bayer (2005) antiswitch S8.
mutable provenance: Adaptation of antiswitch design "S8" by Bayer and Smolke; article available at http://www.nature.com/nbt/journal/v23/n3/full/nbt1069.html
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000834
role: partType/RNA
sequence annotation: Hammerhead Ribozyme
sequence annotation: R0010
sequence annotation: B0015
sequence annotation: EYFP
sequence annotation: Antiswitch
sequence: https://synbiohub.org/public/igem/BBa_J04086_sequence/1

Hammerhead Ribozyme
type: SequenceAnnotation
persistent identity: annotation1759648
display id: annotation1759648
version: 1
top level: BBa_J04086
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J04086/annotation1759648/range1759648/1

https://synbiohub.org/public/igem/BBa_J04086/annotation1759648/range1759648/1
type: Range
persistent identity: range1759648
display id: range1759648
version: 1
direction: direction/forward
top level: BBa_J04086
start: 201
end: 253
orientation: inline

R0010
type: SequenceAnnotation
persistent identity: annotation1759641
display id: annotation1759641
version: 1
top level: BBa_J04086
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_J04086/annotation1759641/range1759641/1

https://synbiohub.org/public/igem/BBa_J04086/annotation1759641/range1759641/1
type: Range
persistent identity: range1759641
display id: range1759641
version: 1
direction: direction/forward
top level: BBa_J04086
start: 1
end: 200
orientation: inline

B0015
type: SequenceAnnotation
persistent identity: annotation1759658
display id: annotation1759658
version: 1
top level: BBa_J04086
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_J04086/annotation1759658/range1759658/1

https://synbiohub.org/public/igem/BBa_J04086/annotation1759658/range1759658/1
type: Range
persistent identity: range1759658
display id: range1759658
version: 1
direction: direction/forward
top level: BBa_J04086
start: 379
end: 507
orientation: inline

EYFP
type: SequenceAnnotation
persistent identity: annotation1759660
display id: annotation1759660
version: 1
top level: BBa_J04086
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J04086/annotation1759660/range1759660/1

https://synbiohub.org/public/igem/BBa_J04086/annotation1759660/range1759660/1
type: Range
persistent identity: range1759660
display id: range1759660
version: 1
direction: direction/forward
top level: BBa_J04086
start: 708
end: 1495
orientation: inline

Antiswitch
type: SequenceAnnotation
persistent identity: annotation1759652
display id: annotation1759652
version: 1
top level: BBa_J04086
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J04086/annotation1759652/range1759652/1

https://synbiohub.org/public/igem/BBa_J04086/annotation1759652/range1759652/1
type: Range
persistent identity: range1759652
display id: range1759652
version: 1
direction: direction/forward
top level: BBa_J04086
start: 254
end: 327
orientation: inline

B0015
type: SequenceAnnotation
persistent identity: annotation1759670
display id: annotation1759670
version: 1
top level: BBa_J04086
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_J04086/annotation1759670/range1759670/1

https://synbiohub.org/public/igem/BBa_J04086/annotation1759670/range1759670/1
type: Range
persistent identity: range1759670
display id: range1759670
version: 1
direction: direction/forward
top level: BBa_J04086
start: 1496
end: 1632
orientation: inline

Hammerhead Ribozyme
type: SequenceAnnotation
persistent identity: annotation1759653
display id: annotation1759653
version: 1
top level: BBa_J04086
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J04086/annotation1759653/range1759653/1

https://synbiohub.org/public/igem/BBa_J04086/annotation1759653/range1759653/1
type: Range
persistent identity: range1759653
display id: range1759653
version: 1
direction: direction/forward
top level: BBa_J04086
start: 328
end: 378
orientation: inline

R0010
type: SequenceAnnotation
persistent identity: annotation1759659
display id: annotation1759659
version: 1
top level: BBa_J04086
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_J04086/annotation1759659/range1759659/1

https://synbiohub.org/public/igem/BBa_J04086/annotation1759659/range1759659/1
type: Range
persistent identity: range1759659
display id: range1759659
version: 1
direction: direction/forward
top level: BBa_J04086
start: 508
end: 707
orientation: inline

BBa_I1044
type: ComponentDefinition
persistent identity: BBa I1044
display id: BBa_I1044
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CI(2) "KISS" asRNA without Promoter
creator: Grace Kenney, Daniel Shen, Neelaksh Varshney, Samantha Sutton
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:30Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Region which serves as basis for transcription of asRNA that binds to and inhibits <bb_part>BBa_I1040</bb_part> mRNA transcript. Part of the XOR gate comprised of <bb_part>BBa_I1030</bb_part> and <bb_part>BBa_I1040</bb_part>, their corresponding asRNA coding sequences (<bb_part>BBa_I1031</bb_part> and <bb_part>BBa_I1041</bb_part>).
mutable notes: Complementary to beginning of <bb_part>BBa_I1040</bb_part> transcript covering junk region, RBS, start codon, and 73 bp into coding sequence. No designed secondary structure. [KEIL96] Incompatible with systems containing <bb_part>BBa_I1042</bb_part> Compatible with <bb_part>BBa_I1020</bb_part>, <bb_part>BBa_I1021</bb_part>, <bb_part>BBa_I1022</bb_part>, <bb_part>BBa_I1023</bb_part>.
mutable provenance: see references
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000834
role: partType/RNA
sequence annotation: antisense region for I1040
sequence annotation: BBa_I1044
sequence: https://synbiohub.org/public/igem/BBa_I1044_sequence/1

antisense region for I1040
type: SequenceAnnotation
persistent identity: annotation1933
display id: annotation1933
version: 1
top level: BBa_I1044
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I1044/annotation1933/range1933/1

https://synbiohub.org/public/igem/BBa_I1044/annotation1933/range1933/1
type: Range
persistent identity: range1933
display id: range1933
version: 1
direction: direction/forward
top level: BBa_I1044
start: 1
end: 108
orientation: inline

BBa_I1044
type: SequenceAnnotation
persistent identity: annotation7058
display id: annotation7058
version: 1
top level: BBa_I1044
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_I1044/annotation7058/range7058/1

https://synbiohub.org/public/igem/BBa_I1044/annotation7058/range7058/1
type: Range
persistent identity: range7058
display id: range7058
version: 1
direction: direction/forward
top level: BBa_I1044
start: 1
end: 108
orientation: inline

BBa_I1060
type: ComponentDefinition
persistent identity: BBa I1060
display id: BBa_I1060
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Custom cI(2) Protein w/LacO-1 Promoter (Used for Anti-sense inhibition)
creator: Grace Kenney, Daniel Shen, Neelaksh Varshney, Samantha Sutton
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:30Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Coding sequence for modified cI protein with LacO-1 promoter for antisense IS10 mRNA regulation.
mutable notes: <bb_part>BBa_I1060</bb_part> Custom cI Protein with LacO-1 Promoter is based on BioBricks <bb_part>BBa_C0050</bb_part> and <bb_part>BBa_R0011</bb_part>. It is designed such that <bb_part>BBa_I1063</bb_part> can be used to interfere with the <bb_part>BBa_1060</bb_part> transcript by anti-sense mRNA binding, in the IS10 method shown below. <bb_part>BBa_I1060</bb_part> and <bb_part>BBa_I1063</bb_part> can act indepently of related biobrick parts <bb_part>BBa_1010</bb_part>, <bb_part>BBa_1011</bb_part>, <bb_part>BBa_1012</bb_part>, <bb_part>BBa_I1030</bb_part>, <bb_part>BBa_I1031</bb_part>, <bb_part>BBa_I1032</bb_part>, <bb_part>BBa_I1041</bb_part>, <bb_part>BBa_I1042</bb_part>, <bb_part>BBa_I1020</bb_part>, <bb_part>BBa_I1023</bb_part> and <bb_part>BBa_1013</bb_part>. Can be used with all parts of the BBa_I system. It is inhibited by BBa_I1063. Incompatible with systems containing cI (wild type)
mutable provenance: Modified from BioBricks <bb_part>BBa_R0011</bb_part>, and <bb_part>BBa_C0051</bb_part>.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000834
role: partType/RNA
sequence annotation: -35
sequence annotation: swap
sequence annotation: C added
sequence annotation: Binds BBa_I1063
sequence annotation: cI lambda
sequence annotation: BBa_I1060
sequence annotation: 2
sequence annotation: -10
sequence annotation: Complemenatry to Antisense mRNA I1063
sequence annotation: stem_loop
sequence annotation: LacO-1 region
sequence annotation: rbs
sequence annotation: SsrA
sequence annotation: start
sequence annotation: LacO-1
sequence annotation: HH117a swap
sequence: https://synbiohub.org/public/igem/BBa_I1060_sequence/1

-35
type: SequenceAnnotation
persistent identity: annotation1947
display id: annotation1947
version: 1
top level: BBa_I1060
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I1060/annotation1947/range1947/1

https://synbiohub.org/public/igem/BBa_I1060/annotation1947/range1947/1
type: Range
persistent identity: range1947
display id: range1947
version: 1
direction: direction/forward
top level: BBa_I1060
start: 21
end: 26
orientation: inline

swap
type: SequenceAnnotation
persistent identity: annotation1959
display id: annotation1959
version: 1
top level: BBa_I1060
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I1060/annotation1959/range1959/1

https://synbiohub.org/public/igem/BBa_I1060/annotation1959/range1959/1
type: Range
persistent identity: range1959
display id: range1959
version: 1
direction: direction/forward
top level: BBa_I1060
start: 79
end: 79
orientation: inline

C added
type: SequenceAnnotation
persistent identity: annotation1956
display id: annotation1956
version: 1
top level: BBa_I1060
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_I1060/annotation1956/range1956/1

https://synbiohub.org/public/igem/BBa_I1060/annotation1956/range1956/1
type: Range
persistent identity: range1956
display id: range1956
version: 1
direction: direction/forward
top level: BBa_I1060
start: 86
end: 88
orientation: inline

Binds BBa_I1063
type: SequenceAnnotation
persistent identity: annotation1957
display id: annotation1957
version: 1
top level: BBa_I1060
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I1060/annotation1957/range1957/1

https://synbiohub.org/public/igem/BBa_I1060/annotation1957/range1957/1
type: Range
persistent identity: range1957
display id: range1957
version: 1
direction: direction/forward
top level: BBa_I1060
start: 89
end: 90
orientation: inline

cI lambda
type: SequenceAnnotation
persistent identity: annotation1946
display id: annotation1946
version: 1
top level: BBa_I1060
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_I1060/annotation1946/range1946/1

https://synbiohub.org/public/igem/BBa_I1060/annotation1946/range1946/1
type: Range
persistent identity: range1946
display id: range1946
version: 1
direction: direction/forward
top level: BBa_I1060
start: 83
end: 835
orientation: inline

BBa_I1060
type: SequenceAnnotation
persistent identity: annotation7060
display id: annotation7060
version: 1
top level: BBa_I1060
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_I1060/annotation7060/range7060/1

https://synbiohub.org/public/igem/BBa_I1060/annotation7060/range7060/1
type: Range
persistent identity: range7060
display id: range7060
version: 1
direction: direction/forward
top level: BBa_I1060
start: 1
end: 835
orientation: inline

2
type: SequenceAnnotation
persistent identity: annotation1951
display id: annotation1951
version: 1
top level: BBa_I1060
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_I1060/annotation1951/range1951/1

https://synbiohub.org/public/igem/BBa_I1060/annotation1951/range1951/1
type: Range
persistent identity: range1951
display id: range1951
version: 1
direction: direction/forward
top level: BBa_I1060
start: 830
end: 835
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1948
display id: annotation1948
version: 1
top level: BBa_I1060
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I1060/annotation1948/range1948/1

https://synbiohub.org/public/igem/BBa_I1060/annotation1948/range1948/1
type: Range
persistent identity: range1948
display id: range1948
version: 1
direction: direction/forward
top level: BBa_I1060
start: 44
end: 49
orientation: inline

swap
type: SequenceAnnotation
persistent identity: annotation1961
display id: annotation1961
version: 1
top level: BBa_I1060
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I1060/annotation1961/range1961/1

https://synbiohub.org/public/igem/BBa_I1060/annotation1961/range1961/1
type: Range
persistent identity: range1961
display id: range1961
version: 1
direction: direction/forward
top level: BBa_I1060
start: 82
end: 82
orientation: inline

Complemenatry to Antisense mRNA I1063
type: SequenceAnnotation
persistent identity: annotation1949
display id: annotation1949
version: 1
top level: BBa_I1060
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I1060/annotation1949/range1949/1

https://synbiohub.org/public/igem/BBa_I1060/annotation1949/range1949/1
type: Range
persistent identity: range1949
display id: range1949
version: 1
direction: direction/forward
top level: BBa_I1060
start: 56
end: 158
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation1942
display id: annotation1942
version: 1
top level: BBa_I1060
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_I1060/annotation1942/range1942/1

https://synbiohub.org/public/igem/BBa_I1060/annotation1942/range1942/1
type: Range
persistent identity: range1942
display id: range1942
version: 1
direction: direction/forward
top level: BBa_I1060
start: 56
end: 85
orientation: inline

LacO-1 region
type: SequenceAnnotation
persistent identity: annotation1952
display id: annotation1952
version: 1
top level: BBa_I1060
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I1060/annotation1952/range1952/1

https://synbiohub.org/public/igem/BBa_I1060/annotation1952/range1952/1
type: Range
persistent identity: range1952
display id: range1952
version: 1
direction: direction/forward
top level: BBa_I1060
start: 1
end: 20
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation1943
display id: annotation1943
version: 1
top level: BBa_I1060
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_I1060/annotation1943/range1943/1

https://synbiohub.org/public/igem/BBa_I1060/annotation1943/range1943/1
type: Range
persistent identity: range1943
display id: range1943
version: 1
direction: direction/forward
top level: BBa_I1060
start: 73
end: 78
orientation: inline

SsrA
type: SequenceAnnotation
persistent identity: annotation1950
display id: annotation1950
version: 1
top level: BBa_I1060
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_I1060/annotation1950/range1950/1

https://synbiohub.org/public/igem/BBa_I1060/annotation1950/range1950/1
type: Range
persistent identity: range1950
display id: range1950
version: 1
direction: direction/forward
top level: BBa_I1060
start: 797
end: 835
orientation: inline

swap
type: SequenceAnnotation
persistent identity: annotation1958
display id: annotation1958
version: 1
top level: BBa_I1060
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I1060/annotation1958/range1958/1

https://synbiohub.org/public/igem/BBa_I1060/annotation1958/range1958/1
type: Range
persistent identity: range1958
display id: range1958
version: 1
direction: direction/forward
top level: BBa_I1060
start: 72
end: 72
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation1945
display id: annotation1945
version: 1
top level: BBa_I1060
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_I1060/annotation1945/range1945/1

https://synbiohub.org/public/igem/BBa_I1060/annotation1945/range1945/1
type: Range
persistent identity: range1945
display id: range1945
version: 1
direction: direction/forward
top level: BBa_I1060
start: 83
end: 85
orientation: inline

swap
type: SequenceAnnotation
persistent identity: annotation1960
display id: annotation1960
version: 1
top level: BBa_I1060
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I1060/annotation1960/range1960/1

https://synbiohub.org/public/igem/BBa_I1060/annotation1960/range1960/1
type: Range
persistent identity: range1960
display id: range1960
version: 1
direction: direction/forward
top level: BBa_I1060
start: 81
end: 81
orientation: inline

LacO-1
type: SequenceAnnotation
persistent identity: annotation1953
display id: annotation1953
version: 1
top level: BBa_I1060
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I1060/annotation1953/range1953/1

https://synbiohub.org/public/igem/BBa_I1060/annotation1953/range1953/1
type: Range
persistent identity: range1953
display id: range1953
version: 1
direction: direction/forward
top level: BBa_I1060
start: 27
end: 43
orientation: inline

HH117a swap
type: SequenceAnnotation
persistent identity: annotation1955
display id: annotation1955
version: 1
top level: BBa_I1060
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I1060/annotation1955/range1955/1

https://synbiohub.org/public/igem/BBa_I1060/annotation1955/range1955/1
type: Range
persistent identity: range1955
display id: range1955
version: 1
direction: direction/forward
top level: BBa_I1060
start: 58
end: 58
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation1954
display id: annotation1954
version: 1
top level: BBa_I1060
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_I1060/annotation1954/range1954/1

https://synbiohub.org/public/igem/BBa_I1060/annotation1954/range1954/1
type: Range
persistent identity: range1954
display id: range1954
version: 1
direction: direction/forward
top level: BBa_I1060
start: 56
end: 56
orientation: inline

BBa_I1011
type: ComponentDefinition
persistent identity: BBa I1011
display id: BBa_I1011
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CI(1) "KISS" asRNA without promoter
creator: June Rhee, Connie Tao, Ty Thomson, Louis Waldman
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:29Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Region which serves as basis for transcription of asRNA that binds to and inhibits <bb_part>BBa_I1010</bb_part>'s mRNA transcript. Has tetR promoter <bb_part>BBa_R0040</bb_part>. Part of the XOR gate comprised of <bb_part>BBa_I1010</bb_part> and <bb_part>BBa_I1020</bb_part>, their corresponding asRNA coding sequences (<bb_part>BBa_I1011</bb_part> and <bb_part>BBa_I1021</bb_part>), and the LacO-1 and TetR <bb_part>BBa_R0040</bb_part> promoters.
mutable notes: References (unparsed) here: Coleman, J., et al. Nature. (1985) 315, 601-3. Pestka, S., et al. Proc. Natl. Acad. Sci. USA (1984) 81, 7525-28. References (unparsed) here: Coleman, J., et al. Nature. (1985) 315, 601-3. Pestka, S., et al. Proc. Natl. Acad. Sci. USA (1984) 81, 7525-28. Complementary to beginning of <bb_part>BBa_I1010</bb_part> transcript covering RBS, start codon, and 73 bp into coding sequence. No designed secondary structure. Anti-sense The success of this system clearly rests on the ability to effectively and specifically target mRNA transcripts for degradation using anti-sense RNA. While many papers, articles, and books have been written on the subject, there are no consensus anti-sense building strategies presented. We thus chose to implement three different types of antisense inhibition: KISS, micRNA, and IS10. In the description that follows, the following nomenclature will be used: target- the mRNA transcript that we wish to inhibit. anti-sense- the anti-sense molecule which will bind and inhibit target. KISS (Keep it SImple, Silly) The simplest of the three methods, this type relies on a single-stranded linear 103 bp anti-sense that is specific to the target of interest. In addition, the first 76 base pairs of the cI region of BBa_I1010 have been codon-modified to give a different sequence that codes for the same cI protein (See BBa_I1030 and I1040). BBa_I1011 contains the reverse complement of the RBS, start codon, and 76 bp region for BBa_I1010. Thus, if both BBa_I1010 and BBa_I1011 are transcribed, the transcripts will bind to each other and BBa_I1010 will not be translated. Note that BBa_I1010 already contains a regulatory region, RBS, and coding region (a terminator must be added), while BBa_I1011 does not - thus, when using this component, the appropriate regulatory region, RBS, and terminator must be added to this part. Incompatible with systems containing <bb_part>BBa_I1012</bb_part>, <bb_part>BBa_I1013</bb_part>. Compatible with <bb_par…
mutable provenance: Custom design.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000834
role: partType/RNA
sequence annotation: BBa_I1011
sequence annotation: Reverse Complement RBS
sequence annotation: Reverse Complement to cI mRNA
sequence annotation: added codon (Cys)
sequence annotation: reverse complement to cI cds
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_I1011_sequence/1

BBa_I1011
type: SequenceAnnotation
persistent identity: annotation7043
display id: annotation7043
version: 1
top level: BBa_I1011
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_I1011/annotation7043/range7043/1

https://synbiohub.org/public/igem/BBa_I1011/annotation7043/range7043/1
type: Range
persistent identity: range7043
display id: range7043
version: 1
direction: direction/forward
top level: BBa_I1011
start: 1
end: 103
orientation: inline

Reverse Complement RBS
type: SequenceAnnotation
persistent identity: annotation1798
display id: annotation1798
version: 1
top level: BBa_I1011
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I1011/annotation1798/range1798/1

https://synbiohub.org/public/igem/BBa_I1011/annotation1798/range1798/1
type: Range
persistent identity: range1798
display id: range1798
version: 1
direction: direction/forward
top level: BBa_I1011
start: 81
end: 86
orientation: inline

Reverse Complement to cI mRNA
type: SequenceAnnotation
persistent identity: annotation1797
display id: annotation1797
version: 1
top level: BBa_I1011
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I1011/annotation1797/range1797/1

https://synbiohub.org/public/igem/BBa_I1011/annotation1797/range1797/1
type: Range
persistent identity: range1797
display id: range1797
version: 1
direction: direction/forward
top level: BBa_I1011
start: 1
end: 103
orientation: inline

added codon (Cys)
type: SequenceAnnotation
persistent identity: annotation1801
display id: annotation1801
version: 1
top level: BBa_I1011
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_I1011/annotation1801/range1801/1

https://synbiohub.org/public/igem/BBa_I1011/annotation1801/range1801/1
type: Range
persistent identity: range1801
display id: range1801
version: 1
direction: direction/forward
top level: BBa_I1011
start: 71
end: 73
orientation: inline

reverse complement to cI cds
type: SequenceAnnotation
persistent identity: annotation1799
display id: annotation1799
version: 1
top level: BBa_I1011
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I1011/annotation1799/range1799/1

https://synbiohub.org/public/igem/BBa_I1011/annotation1799/range1799/1
type: Range
persistent identity: range1799
display id: range1799
version: 1
direction: direction/forward
top level: BBa_I1011
start: 1
end: 73
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation1800
display id: annotation1800
version: 1
top level: BBa_I1011
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_I1011/annotation1800/range1800/1

https://synbiohub.org/public/igem/BBa_I1011/annotation1800/range1800/1
type: Range
persistent identity: range1800
display id: range1800
version: 1
direction: direction/reverse
top level: BBa_I1011
start: 74
end: 76
orientation: inline

Lock 2
type: ComponentDefinition
persistent identity: BBa J01011
display id: BBa_J01011
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Riboregulator Lock 2
creator: Golden Bear
created: 2005-11-05T12:00:00Z
modified: 2015-08-31T04:08:12Z
discontinued: true
dominant: false
group u list: _13_
m user id: 0
owner id: 395
owning group id: 13
sample status: Discontinued
status: status/Deleted
bookmark: false
mutable description: Variant of biobricked version of Isaacs' riboregulator cis repressed lock, crR12, only change is in the address region.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000834
role: partType/RNA
sequence: https://synbiohub.org/public/igem/BBa_J01011_sequence/1

BBa_I1013
type: ComponentDefinition
persistent identity: BBa I1013
display id: BBa_I1013
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CI(1) IS10 asRNA
creator: June Rhee, Connie Tao, Ty Thomson, Louis Waldman
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:29Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Region which serves as basis for transcription of asRNA that binds to and inhibits <bb_part>BBa_I1010</bb_part>'s mRNA transcript via micRNA mechanism (See below for details). Has tetR promoter <bb_part>BBa_R0040</bb_part>. Part of the XOR gate comprised of <bb_part>BBa_I1010</bb_part> and <bb_part>BBa_I1020</bb_part>, their corresponding asRNA coding sequences (<bb_part>BBa_I1013</bb_part> and <bb_part>BBa_I1023</bb_part>), and the LacO-1 and TetR <bb_part>BBa_R0040</bb_part> promoters.
mutable notes: References (unparsed) here: Case, C., Roels, S., Jense, P., Lee, J., Kleckner, N. and Simons, R. (1989). The unusual stability of the IS10 anti-sense RNA is critical for its function and is determined by the structure of its stem-domain. EMBO 8(13): 4297-4305. Jain, C. (1995). IS10 Antisense Control in Vivo is Affected by Mutations Throughout the Region of Complementarity Between the Interacting RNAs. J. Mol. Biol. 246:585-594. Jain, C. (1997). Models for Pairing of IS10 Encoded Antisense RNAs in vivo. J. theor. Biol. 186: 431-439. Kittle, J.D., Simons, R.W., Lee, J., and Kleckner, N. (1989). Insertion Sequence IS10 Anti-sense Pairing Initiates by an Interaction Between the 5' End of the Target RNA and a Loop in the Anti-sense RNA. J. Mol. Biol. 210:561-572. Lutz, R., and Bujard, H. (1997). Independent and tight regulation of transcriptional units in E. coli via the LacR/O, the TetR/O and AraC/I1-I2 regulatory elements. Nucleic Acids Research 25(6): 1203-1210. Ma, C., and Simons, R. (1990). The IS10 antisense RNA blocks ribosome binding at the transposase translation initiation site. EMBO 9(4):1267-1274. E. coli codon usage table (http://bioinfo.weizmann.ac.il:3456/kegg/codon_table/codon_eco.html). References (unparsed) here: Case, C., Roels, S., Jense, P., Lee, J., Kleckner, N. and Simons, R. (1989). The unusual stability of the IS10 anti-sense RNA is critical for its function and is determined by the structure of its stem-domain. EMBO 8(13): 4297-4305. Jain, C. (1995). IS10 Antisense Control in Vivo is Affected by Mutations Throughout the Region of Complementarity Between the Interacting RNAs. J. Mol. Biol. 246:585-594. Jain, C. (1997). Models for Pairing of IS10 Encoded Antisense RNAs in vivo. J. theor. Biol. 186: 431-439. Kittle, J.D., Simons, R.W., Lee, J., and Kleckner, N. (1989). Insertion Sequence IS10 Anti-sense Pairing Initiates by an Interaction Between the 5' End of the Target RNA and a Loop in the Anti-sense RNA. J. Mol. Biol. 210:561-572. Lutz, R., and…
mutable provenance: Custom design.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000834
role: partType/RNA
sequence annotation: Reverse Complement to cI mRNA
sequence annotation: start
sequence annotation: reverse complement to cI cds
sequence annotation: BBa_I1013
sequence annotation: stem_loop
sequence annotation: Reverse Complement RBS
sequence annotation: added codon (Cys)
sequence: https://synbiohub.org/public/igem/BBa_I1013_sequence/1

Reverse Complement to cI mRNA
type: SequenceAnnotation
persistent identity: annotation1810
display id: annotation1810
version: 1
top level: BBa_I1013
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I1013/annotation1810/range1810/1

https://synbiohub.org/public/igem/BBa_I1013/annotation1810/range1810/1
type: Range
persistent identity: range1810
display id: range1810
version: 1
direction: direction/forward
top level: BBa_I1013
start: 1
end: 35
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation1816
display id: annotation1816
version: 1
top level: BBa_I1013
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_I1013/annotation1816/range1816/1

https://synbiohub.org/public/igem/BBa_I1013/annotation1816/range1816/1
type: Range
persistent identity: range1816
display id: range1816
version: 1
direction: direction/reverse
top level: BBa_I1013
start: 6
end: 8
orientation: inline

reverse complement to cI cds
type: SequenceAnnotation
persistent identity: annotation1814
display id: annotation1814
version: 1
top level: BBa_I1013
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I1013/annotation1814/range1814/1

https://synbiohub.org/public/igem/BBa_I1013/annotation1814/range1814/1
type: Range
persistent identity: range1814
display id: range1814
version: 1
direction: direction/forward
top level: BBa_I1013
start: 1
end: 8
orientation: inline

BBa_I1013
type: SequenceAnnotation
persistent identity: annotation7045
display id: annotation7045
version: 1
top level: BBa_I1013
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_I1013/annotation7045/range7045/1

https://synbiohub.org/public/igem/BBa_I1013/annotation7045/range7045/1
type: Range
persistent identity: range7045
display id: range7045
version: 1
direction: direction/forward
top level: BBa_I1013
start: 1
end: 115
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation1811
display id: annotation1811
version: 1
top level: BBa_I1013
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_I1013/annotation1811/range1811/1

https://synbiohub.org/public/igem/BBa_I1013/annotation1811/range1811/1
type: Range
persistent identity: range1811
display id: range1811
version: 1
direction: direction/forward
top level: BBa_I1013
start: 24
end: 44
orientation: inline

Reverse Complement RBS
type: SequenceAnnotation
persistent identity: annotation1813
display id: annotation1813
version: 1
top level: BBa_I1013
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I1013/annotation1813/range1813/1

https://synbiohub.org/public/igem/BBa_I1013/annotation1813/range1813/1
type: Range
persistent identity: range1813
display id: range1813
version: 1
direction: direction/forward
top level: BBa_I1013
start: 13
end: 18
orientation: inline

added codon (Cys)
type: SequenceAnnotation
persistent identity: annotation1818
display id: annotation1818
version: 1
top level: BBa_I1013
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_I1013/annotation1818/range1818/1

https://synbiohub.org/public/igem/BBa_I1013/annotation1818/range1818/1
type: Range
persistent identity: range1818
display id: range1818
version: 1
direction: direction/forward
top level: BBa_I1013
start: 3
end: 5
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation1817
display id: annotation1817
version: 1
top level: BBa_I1013
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_I1013/annotation1817/range1817/1

https://synbiohub.org/public/igem/BBa_I1013/annotation1817/range1817/1
type: Range
persistent identity: range1817
display id: range1817
version: 1
direction: direction/forward
top level: BBa_I1013
start: 3
end: 65
orientation: inline

BBa_I1022
type: ComponentDefinition
persistent identity: BBa I1022
display id: BBa_I1022
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Antisense RNA w/BBa_I1020 Interference (micRNA)
creator: June Rhee, Connie Tao, Ty Thomson, Louis Waldman
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:29Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Sequence for antisense RNA that interferes with BBa_I1020 mRNA using the micRNA scheme.
mutable notes: References (unparsed) here: Mizuno, T., et al. Proc. Natl. Acad. Sci. USA (1984) 81, 1966-1970 Coleman, J., et al. Cell (1984) 37, 429-36 References (unparsed) here: Mizuno, T., et al. Proc. Natl. Acad. Sci. USA (1984) 81, 1966-1970 Coleman, J., et al. Cell (1984) 37, 429-36 <bb_part>BBa_I1022</bb_part> antisense RNA is designed to interfere with <bb_part>BBa_I1020</bb_part> mRNA via the micRNA scheme (Mizuno, T., et al. Proc. Natl. Acad. Sci. USA (1984) 81, 1966-1970). The sequence is designed to interfere exclusively with <bb_part>BBa_I1020</bb_part>, and act independently of <bb_part>BBa_I1010</bb_part> and <bb_part>BBa_I1012</bb_part>. These parts are designed to use codon space to code for the same protein (<bb_part>BBa_I1010</bb_part> and <bb_part>BBa_1020</bb_part> code for lambda cI) while allowing for independent anti-sense regulation of the <bb_part>BBa_I1010</bb_part> and <bb_part>BBa_1020</bb_part> transcripts via <bb_part>BBa_I1012</bb_part> and <bb_part>BBa_1022</bb_part>.
mutable provenance: Anti-sense secondary structure adapted from: Mizuno, T., et al. Proc. Natl. Acad. Sci. USA (1984) 81, 1966-1970
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000834
role: partType/RNA
sequence annotation: BBa_I1022
sequence annotation: stem_loop
sequence annotation: start
sequence annotation: cI mRNA reverse complement
sequence annotation: RBS Reverse Complement
sequence annotation: suffix
sequence annotation: cI CDS Reverse Complement
sequence: https://synbiohub.org/public/igem/BBa_I1022_sequence/1

BBa_I1022
type: SequenceAnnotation
persistent identity: annotation7048
display id: annotation7048
version: 1
top level: BBa_I1022
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_I1022/annotation7048/range7048/1

https://synbiohub.org/public/igem/BBa_I1022/annotation7048/range7048/1
type: Range
persistent identity: range7048
display id: range7048
version: 1
direction: direction/forward
top level: BBa_I1022
start: 1
end: 196
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation1842
display id: annotation1842
version: 1
top level: BBa_I1022
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_I1022/annotation1842/range1842/1

https://synbiohub.org/public/igem/BBa_I1022/annotation1842/range1842/1
type: Range
persistent identity: range1842
display id: range1842
version: 1
direction: direction/forward
top level: BBa_I1022
start: 1
end: 57
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation1845
display id: annotation1845
version: 1
top level: BBa_I1022
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_I1022/annotation1845/range1845/1

https://synbiohub.org/public/igem/BBa_I1022/annotation1845/range1845/1
type: Range
persistent identity: range1845
display id: range1845
version: 1
direction: direction/forward
top level: BBa_I1022
start: 128
end: 130
orientation: inline

cI mRNA reverse complement
type: SequenceAnnotation
persistent identity: annotation1846
display id: annotation1846
version: 1
top level: BBa_I1022
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I1022/annotation1846/range1846/1

https://synbiohub.org/public/igem/BBa_I1022/annotation1846/range1846/1
type: Range
persistent identity: range1846
display id: range1846
version: 1
direction: direction/forward
top level: BBa_I1022
start: 58
end: 157
orientation: inline

RBS Reverse Complement
type: SequenceAnnotation
persistent identity: annotation1848
display id: annotation1848
version: 1
top level: BBa_I1022
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I1022/annotation1848/range1848/1

https://synbiohub.org/public/igem/BBa_I1022/annotation1848/range1848/1
type: Range
persistent identity: range1848
display id: range1848
version: 1
direction: direction/forward
top level: BBa_I1022
start: 135
end: 140
orientation: inline

suffix
type: SequenceAnnotation
persistent identity: annotation1843
display id: annotation1843
version: 1
top level: BBa_I1022
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I1022/annotation1843/range1843/1

https://synbiohub.org/public/igem/BBa_I1022/annotation1843/range1843/1
type: Range
persistent identity: range1843
display id: range1843
version: 1
direction: direction/forward
top level: BBa_I1022
start: 193
end: 196
orientation: inline

cI CDS Reverse Complement
type: SequenceAnnotation
persistent identity: annotation1847
display id: annotation1847
version: 1
top level: BBa_I1022
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I1022/annotation1847/range1847/1

https://synbiohub.org/public/igem/BBa_I1022/annotation1847/range1847/1
type: Range
persistent identity: range1847
display id: range1847
version: 1
direction: direction/forward
top level: BBa_I1022
start: 58
end: 130
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation1844
display id: annotation1844
version: 1
top level: BBa_I1022
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_I1022/annotation1844/range1844/1

https://synbiohub.org/public/igem/BBa_I1022/annotation1844/range1844/1
type: Range
persistent identity: range1844
display id: range1844
version: 1
direction: direction/forward
top level: BBa_I1022
start: 158
end: 192
orientation: inline

BBa_J04805
type: ComponentDefinition
persistent identity: BBa J04805
display id: BBa_J04805
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter with Riboswitch designed to turn "ON" a protein
creator: Kristen DeCelle
created: 2005-09-10T11:00:00Z
modified: 2015-08-31T04:08:15Z
discontinued: false
dominant: false
group u list: _16_
m user id: 0
owner id: 326
owning group id: 16
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: These two parts will hopefully be made by blue heron and be able to be ligated onto RBS, Coding, and Terminator sequences. It is predicted that they will serve to turn "ON" any protein we attach to it with increasing presence of theophylline.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000834
role: partType/RNA
sequence annotation: CAP binding site
sequence annotation: -10
sequence annotation: BBa_J04705
sequence annotation: BBa_R0010
sequence annotation: LacI binding site
sequence annotation: -35
sequence annotation: end of LacI coding region (inactive)
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_J04805_sequence/1

CAP binding site
type: SequenceAnnotation
persistent identity: annotation1687940
display id: annotation1687940
version: 1
top level: BBa_J04805
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_J04805/annotation1687940/range1687940/1

https://synbiohub.org/public/igem/BBa_J04805/annotation1687940/range1687940/1
type: Range
persistent identity: range1687940
display id: range1687940
version: 1
direction: direction/forward
top level: BBa_J04805
start: 89
end: 126
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1687942
display id: annotation1687942
version: 1
top level: BBa_J04805
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_J04805/annotation1687942/range1687942/1

https://synbiohub.org/public/igem/BBa_J04805/annotation1687942/range1687942/1
type: Range
persistent identity: range1687942
display id: range1687942
version: 1
direction: direction/forward
top level: BBa_J04805
start: 161
end: 166
orientation: inline

BBa_J04705
type: SequenceAnnotation
persistent identity: annotation1687946
display id: annotation1687946
version: 1
top level: BBa_J04805
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_J04805/annotation1687946/range1687946/1

https://synbiohub.org/public/igem/BBa_J04805/annotation1687946/range1687946/1
type: Range
persistent identity: range1687946
display id: range1687946
version: 1
direction: direction/forward
top level: BBa_J04805
start: 209
end: 246
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation1687938
display id: annotation1687938
version: 1
top level: BBa_J04805
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_J04805/annotation1687938/range1687938/1

https://synbiohub.org/public/igem/BBa_J04805/annotation1687938/range1687938/1
type: Range
persistent identity: range1687938
display id: range1687938
version: 1
direction: direction/forward
top level: BBa_J04805
start: 1
end: 200
orientation: inline

LacI binding site
type: SequenceAnnotation
persistent identity: annotation1687943
display id: annotation1687943
version: 1
top level: BBa_J04805
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_J04805/annotation1687943/range1687943/1

https://synbiohub.org/public/igem/BBa_J04805/annotation1687943/range1687943/1
type: Range
persistent identity: range1687943
display id: range1687943
version: 1
direction: direction/forward
top level: BBa_J04805
start: 166
end: 200
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1687941
display id: annotation1687941
version: 1
top level: BBa_J04805
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_J04805/annotation1687941/range1687941/1

https://synbiohub.org/public/igem/BBa_J04805/annotation1687941/range1687941/1
type: Range
persistent identity: range1687941
display id: range1687941
version: 1
direction: direction/forward
top level: BBa_J04805
start: 137
end: 142
orientation: inline

end of LacI coding region (inactive)
type: SequenceAnnotation
persistent identity: annotation1687939
display id: annotation1687939
version: 1
top level: BBa_J04805
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J04805/annotation1687939/range1687939/1

https://synbiohub.org/public/igem/BBa_J04805/annotation1687939/range1687939/1
type: Range
persistent identity: range1687939
display id: range1687939
version: 1
direction: direction/forward
top level: BBa_J04805
start: 1
end: 88
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation1687944
display id: annotation1687944
version: 1
top level: BBa_J04805
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_J04805/annotation1687944/range1687944/1

https://synbiohub.org/public/igem/BBa_J04805/annotation1687944/range1687944/1
type: Range
persistent identity: range1687944
display id: range1687944
version: 1
direction: direction/forward
top level: BBa_J04805
start: 173
end: 173
orientation: inline

BBa_I1031
type: ComponentDefinition
persistent identity: BBa I1031
display id: BBa_I1031
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CI(1) "KISS" asRNA
creator: Grace Kenney, Daniel Shen, Neelaksh Varshney, Samantha Sutton
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:29Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Region which serves as basis for transcription of asRNA that binds to and inhibits <bb_part>BBa_I1030</bb_part>'s mRNA transcript. Has LacO-1 regulatory region with promoter. Part of the XOR gate comprised of <bb_part>BBa_I1030</bb_part> and <bb_part>BBa_I1040</bb_part>, their corresponding asRNA coding sequences (<bb_part>BBa_I1011</bb_part> and <bb_part>BBa_I1031</bb_part>).
mutable notes: Complementary to beginning of <bb_part>BBa_I1030</bb_part> transcript covering junk region, RBS, start codon, and 73 bp into coding sequence. No designed secondary structure. [KEIL96] Incompatible with systems containing <bb_part>BBa_I1032</bb_part> Compatible with <bb_part>BBa_I1020</bb_part>, <bb_part>BBa_I1021</bb_part>, <bb_part>BBa_I1022</bb_part>, <bb_part>BBa_I1023</bb_part>.
mutable provenance: see references
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000834
role: partType/RNA
sequence annotation: LacO-1
sequence annotation: antisense region for I1030
sequence annotation: BBa_I1031
sequence: https://synbiohub.org/public/igem/BBa_I1031_sequence/1

LacO-1
type: SequenceAnnotation
persistent identity: annotation1885
display id: annotation1885
version: 1
top level: BBa_I1031
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_I1031/annotation1885/range1885/1

https://synbiohub.org/public/igem/BBa_I1031/annotation1885/range1885/1
type: Range
persistent identity: range1885
display id: range1885
version: 1
direction: direction/forward
top level: BBa_I1031
start: 1
end: 55
orientation: inline

antisense region for I1030
type: SequenceAnnotation
persistent identity: annotation1884
display id: annotation1884
version: 1
top level: BBa_I1031
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I1031/annotation1884/range1884/1

https://synbiohub.org/public/igem/BBa_I1031/annotation1884/range1884/1
type: Range
persistent identity: range1884
display id: range1884
version: 1
direction: direction/forward
top level: BBa_I1031
start: 56
end: 163
orientation: inline

BBa_I1031
type: SequenceAnnotation
persistent identity: annotation7051
display id: annotation7051
version: 1
top level: BBa_I1031
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_I1031/annotation7051/range7051/1

https://synbiohub.org/public/igem/BBa_I1031/annotation7051/range7051/1
type: Range
persistent identity: range7051
display id: range7051
version: 1
direction: direction/forward
top level: BBa_I1031
start: 1
end: 163
orientation: inline

BBa_J04686
type: ComponentDefinition
persistent identity: BBa J04686
display id: BBa_J04686
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Antiswitch repressing production of EYFP ("Off" switch)
creator: Nicholas Cain, Davidson College
created: 2005-06-27T11:00:00Z
modified: 2015-08-31T04:08:15Z
discontinued: false
dominant: false
experience: experience/Fails
group u list: _16_
m user id: 0
owner id: 331
owning group id: 16
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: The presence of theophylline prevents translation of mRNA transcribed from Biobrick BBa_E0032. The absence of theophylline allows expression of the coding region of BBa_E0032.
mutable notes: Transcription of the antiswitch is repressed in the presence of tetR
mutable provenance: Adaptation of antiswitch design "S1" by Bayer and Smolke; article available at http://www.nature.com/nbt/journal/v23/n3/full/nbt1069.html
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000834
role: partType/RNA
sequence annotation: R0010 Promoter Region
sequence annotation: Hammerhead Riobozyme #2
sequence annotation: B0015 Terminator
sequence annotation: "On" Antiswitch
sequence annotation: Hammerhead Riobozyme #1
sequence annotation: Antisense Binding Sequence
sequence: https://synbiohub.org/public/igem/BBa_J04686_sequence/1

R0010 Promoter Region
type: SequenceAnnotation
persistent identity: annotation1553090
display id: annotation1553090
version: 1
top level: BBa_J04686
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_J04686/annotation1553090/range1553090/1

https://synbiohub.org/public/igem/BBa_J04686/annotation1553090/range1553090/1
type: Range
persistent identity: range1553090
display id: range1553090
version: 1
direction: direction/forward
top level: BBa_J04686
start: 1
end: 54
orientation: inline

Hammerhead Riobozyme #2
type: SequenceAnnotation
persistent identity: annotation1553214
display id: annotation1553214
version: 1
top level: BBa_J04686
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_J04686/annotation1553214/range1553214/1

https://synbiohub.org/public/igem/BBa_J04686/annotation1553214/range1553214/1
type: Range
persistent identity: range1553214
display id: range1553214
version: 1
direction: direction/forward
top level: BBa_J04686
start: 182
end: 232
orientation: inline

B0015 Terminator
type: SequenceAnnotation
persistent identity: annotation1553215
display id: annotation1553215
version: 1
top level: BBa_J04686
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J04686/annotation1553215/range1553215/1

https://synbiohub.org/public/igem/BBa_J04686/annotation1553215/range1553215/1
type: Range
persistent identity: range1553215
display id: range1553215
version: 1
direction: direction/forward
top level: BBa_J04686
start: 233
end: 360
orientation: inline

"On" Antiswitch
type: SequenceAnnotation
persistent identity: annotation1553213
display id: annotation1553213
version: 1
top level: BBa_J04686
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J04686/annotation1553213/range1553213/1

https://synbiohub.org/public/igem/BBa_J04686/annotation1553213/range1553213/1
type: Range
persistent identity: range1553213
display id: range1553213
version: 1
direction: direction/forward
top level: BBa_J04686
start: 108
end: 181
orientation: inline

Hammerhead Riobozyme #1
type: SequenceAnnotation
persistent identity: annotation1553095
display id: annotation1553095
version: 1
top level: BBa_J04686
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_J04686/annotation1553095/range1553095/1

https://synbiohub.org/public/igem/BBa_J04686/annotation1553095/range1553095/1
type: Range
persistent identity: range1553095
display id: range1553095
version: 1
direction: direction/forward
top level: BBa_J04686
start: 55
end: 107
orientation: inline

Antisense Binding Sequence
type: SequenceAnnotation
persistent identity: annotation1553216
display id: annotation1553216
version: 1
top level: BBa_J04686
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J04686/annotation1553216/range1553216/1

https://synbiohub.org/public/igem/BBa_J04686/annotation1553216/range1553216/1
type: Range
persistent identity: range1553216
display id: range1553216
version: 1
direction: direction/forward
top level: BBa_J04686
start: 108
end: 122
orientation: inline

BBa_I1012
type: ComponentDefinition
persistent identity: BBa I1012
display id: BBa_I1012
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CI(1) micRNA asRNA
creator: June Rhee, Connie Tao, Ty Thomson, Louis Waldman
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:29Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Region which serves as basis for transcription of asRNA that binds to and inhibits <bb_part>BBa_I1010</bb_part>'s mRNA transcript via micRNA mechanism (See below for details). Has tetR promoter <bb_part>BBa_R0040</bb_part>. Part of the XOR gate comprised of <bb_part>BBa_I1010</bb_part> and <bb_part>BBa_I1020</bb_part>, their corresponding asRNA coding sequences (<bb_part>BBa_I1012</bb_part> and <bb_part>BBa_I1022</bb_part>), and the LacO-1 and TetR <bb_part>BBa_R0040</bb_part> promoters.
mutable notes: References (unparsed) here: Mizuno, T., et al. Proc. Natl. Acad. Sci. USA (1984) 81, 1966-1970. Coleman, J., et al. Cell (1984) 37, 429-36. References (unparsed) here: Mizuno, T., et al. Proc. Natl. Acad. Sci. USA (1984) 81, 1966-1970. Coleman, J., et al. Cell (1984) 37, 429-36. Complementary to beginning of <bb_part>BBa_I1010</bb_part> transcript covering RBS, start codon, and 73 bp into coding sequence. No designed secondary structure. Complementary region is flanked by two stem loop structures, as specified by the micRNA mechanism. Anti-sense The success of this system clearly rests on the ability to effectively and specifically target mRNA transcripts for degradation using anti-sense RNA. While many papers, articles, and books have been written on the subject, there are no consensus anti-sense building strategies presented. We thus chose to implement three different types of antisense inhibition: KISS, micRNA, and IS10. In the description that follows, the following nomenclature will be used: target- the mRNA transcript that we wish to inhibit. anti-sense- the anti-sense molecule which will bind and inhibit target. micRNA This anti-sense mechanism relies on two stem loops flanking an anti-sense sequence that is specific for the target. The function of the stem loops is to maintain the anti-sense region in a quasi-linear state. BBa_I1012 is built in this manner, with a linear region that will bind over the RBS, start codon, and 76 bp of BBa_I1010. Incompatible with systems containing <bb_part>BBa_I1011</bb_part>, <bb_part>BBa_I1013</bb_part>. Compatible with <bb_part>BBa_I1020</bb_part>, <bb_part>BBa_I1021</bb_part>, <bb_part>BBa_I1022</bb_part>, <bb_part>BBa_I1023</bb_part>.
mutable provenance: Custom design.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000834
role: partType/RNA
sequence annotation: Reverse Complement RBS
sequence annotation: stem_loop
sequence annotation: start
sequence annotation: Reverse Complement to cI mRNA
sequence annotation: BBa_I1012
sequence annotation: reverse complement to cI cds
sequence: https://synbiohub.org/public/igem/BBa_I1012_sequence/1

Reverse Complement RBS
type: SequenceAnnotation
persistent identity: annotation1806
display id: annotation1806
version: 1
top level: BBa_I1012
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I1012/annotation1806/range1806/1

https://synbiohub.org/public/igem/BBa_I1012/annotation1806/range1806/1
type: Range
persistent identity: range1806
display id: range1806
version: 1
direction: direction/forward
top level: BBa_I1012
start: 138
end: 143
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation1804
display id: annotation1804
version: 1
top level: BBa_I1012
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_I1012/annotation1804/range1804/1

https://synbiohub.org/public/igem/BBa_I1012/annotation1804/range1804/1
type: Range
persistent identity: range1804
display id: range1804
version: 1
direction: direction/forward
top level: BBa_I1012
start: 161
end: 195
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation1802
display id: annotation1802
version: 1
top level: BBa_I1012
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_I1012/annotation1802/range1802/1

https://synbiohub.org/public/igem/BBa_I1012/annotation1802/range1802/1
type: Range
persistent identity: range1802
display id: range1802
version: 1
direction: direction/forward
top level: BBa_I1012
start: 1
end: 57
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation1809
display id: annotation1809
version: 1
top level: BBa_I1012
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_I1012/annotation1809/range1809/1

https://synbiohub.org/public/igem/BBa_I1012/annotation1809/range1809/1
type: Range
persistent identity: range1809
display id: range1809
version: 1
direction: direction/reverse
top level: BBa_I1012
start: 131
end: 133
orientation: inline

Reverse Complement to cI mRNA
type: SequenceAnnotation
persistent identity: annotation1805
display id: annotation1805
version: 1
top level: BBa_I1012
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I1012/annotation1805/range1805/1

https://synbiohub.org/public/igem/BBa_I1012/annotation1805/range1805/1
type: Range
persistent identity: range1805
display id: range1805
version: 1
direction: direction/forward
top level: BBa_I1012
start: 58
end: 160
orientation: inline

BBa_I1012
type: SequenceAnnotation
persistent identity: annotation7044
display id: annotation7044
version: 1
top level: BBa_I1012
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_I1012/annotation7044/range7044/1

https://synbiohub.org/public/igem/BBa_I1012/annotation7044/range7044/1
type: Range
persistent identity: range7044
display id: range7044
version: 1
direction: direction/forward
top level: BBa_I1012
start: 1
end: 195
orientation: inline

reverse complement to cI cds
type: SequenceAnnotation
persistent identity: annotation1807
display id: annotation1807
version: 1
top level: BBa_I1012
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I1012/annotation1807/range1807/1

https://synbiohub.org/public/igem/BBa_I1012/annotation1807/range1807/1
type: Range
persistent identity: range1807
display id: range1807
version: 1
direction: direction/forward
top level: BBa_I1012
start: 58
end: 133
orientation: inline

BBa_I1033
type: ComponentDefinition
persistent identity: BBa I1033
display id: BBa_I1033
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CI(1) IS10 asRNA
creator: Grace Kenney, Daniel Shen, Neelaksh Varshney, Samantha Sutton
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:29Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Region which serves as basis for transcription of asRNA that binds to and inhibits <bb_part>BBa_I1010</bb_part>'s mRNA transcript. Part of the XOR gate comprised of <bb_part>BBa_I1010</bb_part> and <bb_part>BBa_I1020</bb_part>, their corresponding asRNA coding sequences (<bb_part>BBa_I1033</bb_part> and <bb_part>BBa_I1023</bb_part>).
mutable notes: References (unparsed) here: A synthetic oscillatory network of transcriptional regulators , Elowitz M.B. , Leibler S., Nature(403),335-38: 2000 Case, C., Roels, S., Jense, P., Lee, J., Kleckner, N. and Simons, R. (1989). The unusual stability of the IS10 anti-sense RNA is critical for its function and is determined by the structure of its stem-domain. EMBO 8(13): 4297-4305. Jain, C. (1995). IS10 Antisense Control in Vivo is Affected by Mutations Throughout the Region of Complementarity Between the Interacting RNAs. J. Mol. Biol. 246:585-594. Kittle, J.D., Simons, R.W., Lee, J., and Kleckner, N. (1989). Insertion Sequence IS10 Anti-sense Pairing Initiates by an Interaction Between the 5' End of the Target RNA and a Loop in the Anti-sense RNA. J. Mol. Biol. 210:561-572. Jain, C. (1997). Models for Pairing of IS10 Encoded Antisense RNAs in vivo. J. theor. Biol. 186: 431-439. Lutz, R., and Bujard, H. (1997). Independent and tight regulation of transcriptional units in E. coli via the LacR/O, the TetR/O and AraC/I1-I2 regulatory elements. Nucleic Acids Research 25(6): 1203-1210. Ma, C., and Simons, R. (1990). The IS10 antisense RNA blocks ribosome binding at the transposase translation initiation site. EMBO 9(4):1267-1274. E. coli codon usage table at http://bioinfo.weizmann.ac.il:3456/kegg/codon_table/codon_eco.html. References (unparsed) here: A synthetic oscillatory network of transcriptional regulators , Elowitz M.B. , Leibler S., Nature(403),335-38: 2000 Case, C., Roels, S., Jense, P., Lee, J., Kleckner, N. and Simons, R. (1989). The unusual stability of the IS10 anti-sense RNA is critical for its function and is determined by the structure of its stem-domain. EMBO 8(13): 4297-4305. Jain, C. (1995). IS10 Antisense Control in Vivo is Affected by Mutations Throughout the Region of Complementarity Between the Interacting RNAs. J. Mol. Biol. 246:585-594. Kittle, J.D., Simons, R.W., Lee, J., and Kleckner, N. (1989). Insertion Sequence IS10 Anti-sense Pairing Initiates by…
mutable provenance: see referencers
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000834
role: partType/RNA
sequence annotation: start
sequence annotation: Reverse Complement RBS
sequence annotation: stem_loop
sequence annotation: Reverse Complement to cI mRNA
sequence annotation: BBa_I1033
sequence annotation: reverse complement to cI cds
sequence annotation: added codon (Cys)
sequence annotation: LacO-1
sequence: https://synbiohub.org/public/igem/BBa_I1033_sequence/1

start
type: SequenceAnnotation
persistent identity: annotation1901
display id: annotation1901
version: 1
top level: BBa_I1033
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_I1033/annotation1901/range1901/1

https://synbiohub.org/public/igem/BBa_I1033/annotation1901/range1901/1
type: Range
persistent identity: range1901
display id: range1901
version: 1
direction: direction/forward
top level: BBa_I1033
start: 56
end: 56
orientation: inline

Reverse Complement RBS
type: SequenceAnnotation
persistent identity: annotation1895
display id: annotation1895
version: 1
top level: BBa_I1033
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I1033/annotation1895/range1895/1

https://synbiohub.org/public/igem/BBa_I1033/annotation1895/range1895/1
type: Range
persistent identity: range1895
display id: range1895
version: 1
direction: direction/forward
top level: BBa_I1033
start: 68
end: 73
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation1899
display id: annotation1899
version: 1
top level: BBa_I1033
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_I1033/annotation1899/range1899/1

https://synbiohub.org/public/igem/BBa_I1033/annotation1899/range1899/1
type: Range
persistent identity: range1899
display id: range1899
version: 1
direction: direction/forward
top level: BBa_I1033
start: 58
end: 120
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation1898
display id: annotation1898
version: 1
top level: BBa_I1033
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_I1033/annotation1898/range1898/1

https://synbiohub.org/public/igem/BBa_I1033/annotation1898/range1898/1
type: Range
persistent identity: range1898
display id: range1898
version: 1
direction: direction/reverse
top level: BBa_I1033
start: 61
end: 63
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation1893
display id: annotation1893
version: 1
top level: BBa_I1033
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_I1033/annotation1893/range1893/1

https://synbiohub.org/public/igem/BBa_I1033/annotation1893/range1893/1
type: Range
persistent identity: range1893
display id: range1893
version: 1
direction: direction/forward
top level: BBa_I1033
start: 79
end: 99
orientation: inline

Reverse Complement to cI mRNA
type: SequenceAnnotation
persistent identity: annotation1892
display id: annotation1892
version: 1
top level: BBa_I1033
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I1033/annotation1892/range1892/1

https://synbiohub.org/public/igem/BBa_I1033/annotation1892/range1892/1
type: Range
persistent identity: range1892
display id: range1892
version: 1
direction: direction/forward
top level: BBa_I1033
start: 56
end: 90
orientation: inline

BBa_I1033
type: SequenceAnnotation
persistent identity: annotation7053
display id: annotation7053
version: 1
top level: BBa_I1033
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_I1033/annotation7053/range7053/1

https://synbiohub.org/public/igem/BBa_I1033/annotation7053/range7053/1
type: Range
persistent identity: range7053
display id: range7053
version: 1
direction: direction/forward
top level: BBa_I1033
start: 1
end: 170
orientation: inline

reverse complement to cI cds
type: SequenceAnnotation
persistent identity: annotation1896
display id: annotation1896
version: 1
top level: BBa_I1033
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I1033/annotation1896/range1896/1

https://synbiohub.org/public/igem/BBa_I1033/annotation1896/range1896/1
type: Range
persistent identity: range1896
display id: range1896
version: 1
direction: direction/forward
top level: BBa_I1033
start: 56
end: 63
orientation: inline

added codon (Cys)
type: SequenceAnnotation
persistent identity: annotation1900
display id: annotation1900
version: 1
top level: BBa_I1033
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_I1033/annotation1900/range1900/1

https://synbiohub.org/public/igem/BBa_I1033/annotation1900/range1900/1
type: Range
persistent identity: range1900
display id: range1900
version: 1
direction: direction/forward
top level: BBa_I1033
start: 58
end: 60
orientation: inline

LacO-1
type: SequenceAnnotation
persistent identity: annotation1902
display id: annotation1902
version: 1
top level: BBa_I1033
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_I1033/annotation1902/range1902/1

https://synbiohub.org/public/igem/BBa_I1033/annotation1902/range1902/1
type: Range
persistent identity: range1902
display id: range1902
version: 1
direction: direction/forward
top level: BBa_I1033
start: 1
end: 55
orientation: inline

BBa_I1040
type: ComponentDefinition
persistent identity: BBa I1040
display id: BBa_I1040
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RNA Regulation Target ( Promoter driven by C0010 and cI(2) CDS)
creator: Grace Kenney, Daniel Shen, Neelaksh Varshney, Samantha Sutton
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:29Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Coding region for the LacO-1 promoter with antisense binding region for <bb_part>BBa_I1041</bb_part> and <bb_part>BBa_I1042</bb_part>, and a codon modified cI(1) protein with an LVA degradation tailCoding region for the LacI protein with an LVA degradation tail and without an RBS. LacI binds to the pLac regulator (<bb_part>BBa_R0010</bb_part>) and hybrid Pl-lac01 regulator as is used in parts <bb_part>BBa_I1020</bb_part>, <bb_part>BBa_I1023</bb_part>, <bb_part>BBa_I1031</bb_part>, <bb_part>BBa_I1032</bb_part>, <bb_part>BBa_I1040</bb_part>, <bb_part>BBa_I1041</bb_part>, <bb_part>BBa_I1042</bb_part>, <bb_part>BBa_I1060</bb_part>, <bb_part>BBa_I1063</bb_part>, <bb_part>BBa_I1020</bb_part>. IPTG (Isopropylthiogalactoside) binds to LacI and inhibits its operation, thus allowing transcription. This is the basis of an "Implies" gate.
mutable notes: <bb_part>BBa_I1040</bb_part> Custom cI Protein with LacO-1 Promoter is based on BioBricks <bb_part>BBa_C0050</bb_part>. It is designed such that <bb_part>BBa_I1041</bb_part> and <bb_part>BBa_I1042</bb_part> can be used to interfere with the <bb_part>BBa_1040</bb_part> transcript by anti-sense mRNA binding, in the KISS or micRNA method shown below. <bb_part>BBa_I1040</bb_part> and <bb_part>BBa_I1041</bb_part> or <bb_part>BBa_I1042</bb_part> can act indepently of related biobrick parts <bb_part>BBa_I1031</bb_part>, <bb_part>BBa_I1032</bb_part>, <bb_part>BBa_I1020</bb_part>, and <bb_part>BBa_1013</bb_part>. [KEIL96] Incompatible with systems containing IPTG, lactose, lacI, or cI. May be some cross talk with systems containing <bb_part>BBa_I1020</bb_part> or <bb_part>BBa_I1060</bb_part>. Compatible with all other BBa_line I-line parts. Proper operation requires AMP/CAP complex, which should normally be present in the cell.
mutable provenance: see references
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000834
role: partType/RNA
sequence annotation: cI lambda
sequence annotation: LacO-1 region
sequence annotation: BBa_I1040
sequence annotation: intentional junk dna
sequence annotation: start
sequence annotation: antisense RNA pairing
sequence annotation: LacO-1
sequence annotation: -35
sequence annotation: 2
sequence annotation: -10
sequence annotation: SsrA
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_I1040_sequence/1

cI lambda
type: SequenceAnnotation
persistent identity: annotation1909
display id: annotation1909
version: 1
top level: BBa_I1040
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_I1040/annotation1909/range1909/1

https://synbiohub.org/public/igem/BBa_I1040/annotation1909/range1909/1
type: Range
persistent identity: range1909
display id: range1909
version: 1
direction: direction/forward
top level: BBa_I1040
start: 92
end: 841
orientation: inline

LacO-1 region
type: SequenceAnnotation
persistent identity: annotation1914
display id: annotation1914
version: 1
top level: BBa_I1040
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I1040/annotation1914/range1914/1

https://synbiohub.org/public/igem/BBa_I1040/annotation1914/range1914/1
type: Range
persistent identity: range1914
display id: range1914
version: 1
direction: direction/forward
top level: BBa_I1040
start: 1
end: 20
orientation: inline

BBa_I1040
type: SequenceAnnotation
persistent identity: annotation7055
display id: annotation7055
version: 1
top level: BBa_I1040
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_I1040/annotation7055/range7055/1

https://synbiohub.org/public/igem/BBa_I1040/annotation7055/range7055/1
type: Range
persistent identity: range7055
display id: range7055
version: 1
direction: direction/forward
top level: BBa_I1040
start: 1
end: 841
orientation: inline

intentional junk dna
type: SequenceAnnotation
persistent identity: annotation1919
display id: annotation1919
version: 1
top level: BBa_I1040
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I1040/annotation1919/range1919/1

https://synbiohub.org/public/igem/BBa_I1040/annotation1919/range1919/1
type: Range
persistent identity: range1919
display id: range1919
version: 1
direction: direction/forward
top level: BBa_I1040
start: 86
end: 89
orientation: inline

intentional junk dna
type: SequenceAnnotation
persistent identity: annotation1917
display id: annotation1917
version: 1
top level: BBa_I1040
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I1040/annotation1917/range1917/1

https://synbiohub.org/public/igem/BBa_I1040/annotation1917/range1917/1
type: Range
persistent identity: range1917
display id: range1917
version: 1
direction: direction/forward
top level: BBa_I1040
start: 56
end: 75
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation1908
display id: annotation1908
version: 1
top level: BBa_I1040
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_I1040/annotation1908/range1908/1

https://synbiohub.org/public/igem/BBa_I1040/annotation1908/range1908/1
type: Range
persistent identity: range1908
display id: range1908
version: 1
direction: direction/forward
top level: BBa_I1040
start: 92
end: 94
orientation: inline

antisense RNA pairing
type: SequenceAnnotation
persistent identity: annotation1920
display id: annotation1920
version: 1
top level: BBa_I1040
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I1040/annotation1920/range1920/1

https://synbiohub.org/public/igem/BBa_I1040/annotation1920/range1920/1
type: Range
persistent identity: range1920
display id: range1920
version: 1
direction: direction/forward
top level: BBa_I1040
start: 56
end: 163
orientation: inline

LacO-1
type: SequenceAnnotation
persistent identity: annotation1915
display id: annotation1915
version: 1
top level: BBa_I1040
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I1040/annotation1915/range1915/1

https://synbiohub.org/public/igem/BBa_I1040/annotation1915/range1915/1
type: Range
persistent identity: range1915
display id: range1915
version: 1
direction: direction/forward
top level: BBa_I1040
start: 27
end: 43
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1910
display id: annotation1910
version: 1
top level: BBa_I1040
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I1040/annotation1910/range1910/1

https://synbiohub.org/public/igem/BBa_I1040/annotation1910/range1910/1
type: Range
persistent identity: range1910
display id: range1910
version: 1
direction: direction/forward
top level: BBa_I1040
start: 21
end: 26
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation1916
display id: annotation1916
version: 1
top level: BBa_I1040
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_I1040/annotation1916/range1916/1

https://synbiohub.org/public/igem/BBa_I1040/annotation1916/range1916/1
type: Range
persistent identity: range1916
display id: range1916
version: 1
direction: direction/forward
top level: BBa_I1040
start: 56
end: 56
orientation: inline

2
type: SequenceAnnotation
persistent identity: annotation1913
display id: annotation1913
version: 1
top level: BBa_I1040
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_I1040/annotation1913/range1913/1

https://synbiohub.org/public/igem/BBa_I1040/annotation1913/range1913/1
type: Range
persistent identity: range1913
display id: range1913
version: 1
direction: direction/forward
top level: BBa_I1040
start: 836
end: 841
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1911
display id: annotation1911
version: 1
top level: BBa_I1040
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I1040/annotation1911/range1911/1

https://synbiohub.org/public/igem/BBa_I1040/annotation1911/range1911/1
type: Range
persistent identity: range1911
display id: range1911
version: 1
direction: direction/forward
top level: BBa_I1040
start: 44
end: 49
orientation: inline

SsrA
type: SequenceAnnotation
persistent identity: annotation1912
display id: annotation1912
version: 1
top level: BBa_I1040
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_I1040/annotation1912/range1912/1

https://synbiohub.org/public/igem/BBa_I1040/annotation1912/range1912/1
type: Range
persistent identity: range1912
display id: range1912
version: 1
direction: direction/forward
top level: BBa_I1040
start: 803
end: 841
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation1918
display id: annotation1918
version: 1
top level: BBa_I1040
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_I1040/annotation1918/range1918/1

https://synbiohub.org/public/igem/BBa_I1040/annotation1918/range1918/1
type: Range
persistent identity: range1918
display id: range1918
version: 1
direction: direction/forward
top level: BBa_I1040
start: 78
end: 83
orientation: inline

BBa_I1023
type: ComponentDefinition
persistent identity: BBa I1023
display id: BBa_I1023
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CI(2) IS10 asRNA
creator: Grace Kenney, Neelaksh Varshney, Samantha Sutton , Daniel Shen
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:29Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Region which serves as basis for transcription of asRNA that binds to and inhibits <bb_part>BBa_I1020</bb_part>'s mRNA transcript. Part of the XOR gate comprised of <bb_part>BBa_I1010</bb_part> and <bb_part>BBa_I1020</bb_part>, their corresponding asRNA coding sequences (<bb_part>BBa_I1013</bb_part> and <bb_part>BBa_I1023</bb_part>).
mutable notes: References (unparsed) here: A synthetic oscillatory network of transcriptional regulators , Elowitz M.B. , Leibler S., Nature(403),335-38: 2000 Case, C., Roels, S., Jense, P., Lee, J., Kleckner, N. and Simons, R. (1989). The unusual stability of the IS10 anti-sense RNA is critical for its function and is determined by the structure of its stem-domain. EMBO 8(13): 4297-4305. Jain, C. (1995). IS10 Antisense Control in Vivo is Affected by Mutations Throughout the Region of Complementarity Between the Interacting RNAs. J. Mol. Biol. 246:585-594. Kittle, J.D., Simons, R.W., Lee, J., and Kleckner, N. (1989). Insertion Sequence IS10 Anti-sense Pairing Initiates by an Interaction Between the 5' End of the Target RNA and a Loop in the Anti-sense RNA. J. Mol. Biol. 210:561-572. Jain, C. (1997). Models for Pairing of IS10 Encoded Antisense RNAs in vivo. J. theor. Biol. 186: 431-439. Lutz, R., and Bujard, H. (1997). Independent and tight regulation of transcriptional units in E. coli via the LacR/O, the TetR/O and AraC/I1-I2 regulatory elements. Nucleic Acids Research 25(6): 1203-1210. Ma, C., and Simons, R. (1990). The IS10 antisense RNA blocks ribosome binding at the transposase translation initiation site. EMBO 9(4):1267-1274. E. coli codon usage table at http://bioinfo.weizmann.ac.il:3456/kegg/codon_table/codon_eco.html. References (unparsed) here: A synthetic oscillatory network of transcriptional regulators , Elowitz M.B. , Leibler S., Nature(403),335-38: 2000 Case, C., Roels, S., Jense, P., Lee, J., Kleckner, N. and Simons, R. (1989). The unusual stability of the IS10 anti-sense RNA is critical for its function and is determined by the structure of its stem-domain. EMBO 8(13): 4297-4305. Jain, C. (1995). IS10 Antisense Control in Vivo is Affected by Mutations Throughout the Region of Complementarity Between the Interacting RNAs. J. Mol. Biol. 246:585-594. Kittle, J.D., Simons, R.W., Lee, J., and Kleckner, N. (1989). Insertion Sequence IS10 Anti-sense Pairing Initiates by…
mutable provenance: see referencers
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000834
role: partType/RNA
sequence annotation: stem_loop
sequence annotation: BBa_I1023
sequence annotation: First 2 bp of cI I1020
sequence annotation: -35
sequence annotation: HH117a mutation specific to I1020 and I1023
sequence annotation: RBS Reverse Complement
sequence annotation: TetR 2
sequence annotation: TetR
sequence annotation: Extra Met for stem homology
sequence annotation: start
sequence annotation: TetR 1
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_I1023_sequence/1

stem_loop
type: SequenceAnnotation
persistent identity: annotation1855
display id: annotation1855
version: 1
top level: BBa_I1023
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_I1023/annotation1855/range1855/1

https://synbiohub.org/public/igem/BBa_I1023/annotation1855/range1855/1
type: Range
persistent identity: range1855
display id: range1855
version: 1
direction: direction/forward
top level: BBa_I1023
start: 60
end: 116
orientation: inline

BBa_I1023
type: SequenceAnnotation
persistent identity: annotation7049
display id: annotation7049
version: 1
top level: BBa_I1023
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_I1023/annotation7049/range7049/1

https://synbiohub.org/public/igem/BBa_I1023/annotation7049/range7049/1
type: Range
persistent identity: range7049
display id: range7049
version: 1
direction: direction/forward
top level: BBa_I1023
start: 1
end: 169
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation1850
display id: annotation1850
version: 1
top level: BBa_I1023
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_I1023/annotation1850/range1850/1

https://synbiohub.org/public/igem/BBa_I1023/annotation1850/range1850/1
type: Range
persistent identity: range1850
display id: range1850
version: 1
direction: direction/forward
top level: BBa_I1023
start: 78
end: 98
orientation: inline

First 2 bp of cI I1020
type: SequenceAnnotation
persistent identity: annotation1858
display id: annotation1858
version: 1
top level: BBa_I1023
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I1023/annotation1858/range1858/1

https://synbiohub.org/public/igem/BBa_I1023/annotation1858/range1858/1
type: Range
persistent identity: range1858
display id: range1858
version: 1
direction: direction/forward
top level: BBa_I1023
start: 55
end: 56
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1861
display id: annotation1861
version: 1
top level: BBa_I1023
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I1023/annotation1861/range1861/1

https://synbiohub.org/public/igem/BBa_I1023/annotation1861/range1861/1
type: Range
persistent identity: range1861
display id: range1861
version: 1
direction: direction/forward
top level: BBa_I1023
start: 20
end: 25
orientation: inline

HH117a mutation specific to I1020 and I1023
type: SequenceAnnotation
persistent identity: annotation1856
display id: annotation1856
version: 1
top level: BBa_I1023
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_I1023/annotation1856/range1856/1

https://synbiohub.org/public/igem/BBa_I1023/annotation1856/range1856/1
type: Range
persistent identity: range1856
display id: range1856
version: 1
direction: direction/forward
top level: BBa_I1023
start: 87
end: 87
orientation: inline

RBS Reverse Complement
type: SequenceAnnotation
persistent identity: annotation1852
display id: annotation1852
version: 1
top level: BBa_I1023
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I1023/annotation1852/range1852/1

https://synbiohub.org/public/igem/BBa_I1023/annotation1852/range1852/1
type: Range
persistent identity: range1852
display id: range1852
version: 1
direction: direction/forward
top level: BBa_I1023
start: 67
end: 72
orientation: inline

TetR 2
type: SequenceAnnotation
persistent identity: annotation1862
display id: annotation1862
version: 1
top level: BBa_I1023
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I1023/annotation1862/range1862/1

https://synbiohub.org/public/igem/BBa_I1023/annotation1862/range1862/1
type: Range
persistent identity: range1862
display id: range1862
version: 1
direction: direction/forward
top level: BBa_I1023
start: 26
end: 44
orientation: inline

TetR
type: SequenceAnnotation
persistent identity: annotation1864
display id: annotation1864
version: 1
top level: BBa_I1023
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I1023/annotation1864/range1864/1

https://synbiohub.org/public/igem/BBa_I1023/annotation1864/range1864/1
type: Range
persistent identity: range1864
display id: range1864
version: 1
direction: direction/forward
top level: BBa_I1023
start: 1
end: 54
orientation: inline

Extra Met for stem homology
type: SequenceAnnotation
persistent identity: annotation1857
display id: annotation1857
version: 1
top level: BBa_I1023
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_I1023/annotation1857/range1857/1

https://synbiohub.org/public/igem/BBa_I1023/annotation1857/range1857/1
type: Range
persistent identity: range1857
display id: range1857
version: 1
direction: direction/forward
top level: BBa_I1023
start: 57
end: 59
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation1854
display id: annotation1854
version: 1
top level: BBa_I1023
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_I1023/annotation1854/range1854/1

https://synbiohub.org/public/igem/BBa_I1023/annotation1854/range1854/1
type: Range
persistent identity: range1854
display id: range1854
version: 1
direction: direction/reverse
top level: BBa_I1023
start: 60
end: 62
orientation: inline

TetR 1
type: SequenceAnnotation
persistent identity: annotation1860
display id: annotation1860
version: 1
top level: BBa_I1023
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I1023/annotation1860/range1860/1

https://synbiohub.org/public/igem/BBa_I1023/annotation1860/range1860/1
type: Range
persistent identity: range1860
display id: range1860
version: 1
direction: direction/forward
top level: BBa_I1023
start: 1
end: 19
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1863
display id: annotation1863
version: 1
top level: BBa_I1023
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I1023/annotation1863/range1863/1

https://synbiohub.org/public/igem/BBa_I1023/annotation1863/range1863/1
type: Range
persistent identity: range1863
display id: range1863
version: 1
direction: direction/forward
top level: BBa_I1023
start: 43
end: 48
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation1859
display id: annotation1859
version: 1
top level: BBa_I1023
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_I1023/annotation1859/range1859/1

https://synbiohub.org/public/igem/BBa_I1023/annotation1859/range1859/1
type: Range
persistent identity: range1859
display id: range1859
version: 1
direction: direction/forward
top level: BBa_I1023
start: 55
end: 55
orientation: inline

BBa_I1041
type: ComponentDefinition
persistent identity: BBa I1041
display id: BBa_I1041
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CI(2) "KISS" asRNA
creator: Grace Kenney, Daniel Shen, Neelaksh Varshney, Samantha Sutton
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:29Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Region which serves as basis for transcription of asRNA that binds to and inhibits <bb_part>BBa_I1040</bb_part> mRNA transcript. Has tetR regulatory region and promoter. Part of the XOR gate comprised of <bb_part>BBa_I1030</bb_part> and <bb_part>BBa_I1040</bb_part>, their corresponding asRNA coding sequences (<bb_part>BBa_I1031</bb_part> and <bb_part>BBa_I1041</bb_part>).
mutable notes: Complementary to beginning of <bb_part>BBa_I1040</bb_part> transcript covering junk region, RBS, start codon, and 73 bp into coding sequence. No designed secondary structure. [KEIL96] Incompatible with systems containing <bb_part>BBa_I1042</bb_part> Compatible with <bb_part>BBa_I1020</bb_part>, <bb_part>BBa_I1021</bb_part>, <bb_part>BBa_I1022</bb_part>, <bb_part>BBa_I1023</bb_part>.
mutable provenance: see references
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000834
role: partType/RNA
sequence annotation: BBa_I1041
sequence annotation: antisense region for I1040
sequence annotation: tetR
sequence: https://synbiohub.org/public/igem/BBa_I1041_sequence/1

BBa_I1041
type: SequenceAnnotation
persistent identity: annotation7056
display id: annotation7056
version: 1
top level: BBa_I1041
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_I1041/annotation7056/range7056/1

https://synbiohub.org/public/igem/BBa_I1041/annotation7056/range7056/1
type: Range
persistent identity: range7056
display id: range7056
version: 1
direction: direction/forward
top level: BBa_I1041
start: 1
end: 162
orientation: inline

antisense region for I1040
type: SequenceAnnotation
persistent identity: annotation1923
display id: annotation1923
version: 1
top level: BBa_I1041
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I1041/annotation1923/range1923/1

https://synbiohub.org/public/igem/BBa_I1041/annotation1923/range1923/1
type: Range
persistent identity: range1923
display id: range1923
version: 1
direction: direction/forward
top level: BBa_I1041
start: 55
end: 162
orientation: inline

tetR
type: SequenceAnnotation
persistent identity: annotation1924
display id: annotation1924
version: 1
top level: BBa_I1041
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_I1041/annotation1924/range1924/1

https://synbiohub.org/public/igem/BBa_I1041/annotation1924/range1924/1
type: Range
persistent identity: range1924
display id: range1924
version: 1
direction: direction/forward
top level: BBa_I1041
start: 1
end: 54
orientation: inline

BBa_I1030
type: ComponentDefinition
persistent identity: BBa I1030
display id: BBa_I1030
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RNA Regulation Target ( Promoter driven by C0040 and cI mod #1 CDS)
creator: Grace Kenney, Daniel Shen, Neelaksh Varshney, Samantha Sutton
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:29Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Coding region for the tetR promoter (<bb_part>BBa_C0040</bb_part>) with antisense binding region for <bb_part>BBa_I1031</bb_part> and <bb_part>BBa_I1032</bb_part>, and a codon modified cI(1) protein with an LVA degradation tail.
mutable notes: <bb_part>BBa_I1030</bb_part> Custom cI Protein with TetR Promoter is based on BioBricks <bb_part>BBa_C0050</bb_part> and <bb_part>BBa_R0040</bb_part>. It is designed such that <bb_part>BBa_I1031</bb_part> and <bb_part>BBa_I1032</bb_part>can be used to interfere with the <bb_part>BBa_1030</bb_part> transcript by anti-sense mRNA binding, in the KISS or micRNA method shown below. <bb_part>BBa_I1030</bb_part> and <bb_part>BBa_I1031</bb_part> or <bb_part>BBa_I1032</bb_part> can act indepently of related biobrick parts <bb_part>BBa_I1041</bb_part>, <bb_part>BBa_I1042</bb_part>, <bb_part>BBa_I1020</bb_part>, <bb_part>BBa_BBa_I1023</bb_part> and <bb_part>BBa_1013</bb_part>. [KEIL96] Incompatible with systems containing IPTG, or cI. May be some cross talk with systems containing <bb_part>BBa_I1010</bb_part>. Compatible with all other BBa_line I-line parts. Proper operation requires AMP/CAP complex, which should normally be present in the cell.
mutable provenance: see references
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000834
role: partType/RNA
sequence annotation: antisense RNA pairing
sequence annotation: intentional junk dna
sequence annotation: modified cI1 cds
sequence annotation: -10
sequence annotation: TetR 2
sequence annotation: TetR 1
sequence annotation: cI
sequence annotation: TetR
sequence annotation: rbs
sequence annotation: BBa_I1030
sequence annotation: 2
sequence annotation: -35
sequence annotation: start
sequence annotation: SsrA
sequence: https://synbiohub.org/public/igem/BBa_I1030_sequence/1

antisense RNA pairing
type: SequenceAnnotation
persistent identity: annotation1881
display id: annotation1881
version: 1
top level: BBa_I1030
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I1030/annotation1881/range1881/1

https://synbiohub.org/public/igem/BBa_I1030/annotation1881/range1881/1
type: Range
persistent identity: range1881
display id: range1881
version: 1
direction: direction/forward
top level: BBa_I1030
start: 55
end: 162
orientation: inline

intentional junk dna
type: SequenceAnnotation
persistent identity: annotation1880
display id: annotation1880
version: 1
top level: BBa_I1030
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I1030/annotation1880/range1880/1

https://synbiohub.org/public/igem/BBa_I1030/annotation1880/range1880/1
type: Range
persistent identity: range1880
display id: range1880
version: 1
direction: direction/forward
top level: BBa_I1030
start: 85
end: 88
orientation: inline

modified cI1 cds
type: SequenceAnnotation
persistent identity: annotation1868
display id: annotation1868
version: 1
top level: BBa_I1030
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_I1030/annotation1868/range1868/1

https://synbiohub.org/public/igem/BBa_I1030/annotation1868/range1868/1
type: Range
persistent identity: range1868
display id: range1868
version: 1
direction: direction/forward
top level: BBa_I1030
start: 94
end: 163
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1872
display id: annotation1872
version: 1
top level: BBa_I1030
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I1030/annotation1872/range1872/1

https://synbiohub.org/public/igem/BBa_I1030/annotation1872/range1872/1
type: Range
persistent identity: range1872
display id: range1872
version: 1
direction: direction/forward
top level: BBa_I1030
start: 43
end: 48
orientation: inline

TetR 2
type: SequenceAnnotation
persistent identity: annotation1871
display id: annotation1871
version: 1
top level: BBa_I1030
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I1030/annotation1871/range1871/1

https://synbiohub.org/public/igem/BBa_I1030/annotation1871/range1871/1
type: Range
persistent identity: range1871
display id: range1871
version: 1
direction: direction/forward
top level: BBa_I1030
start: 26
end: 44
orientation: inline

TetR 1
type: SequenceAnnotation
persistent identity: annotation1869
display id: annotation1869
version: 1
top level: BBa_I1030
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I1030/annotation1869/range1869/1

https://synbiohub.org/public/igem/BBa_I1030/annotation1869/range1869/1
type: Range
persistent identity: range1869
display id: range1869
version: 1
direction: direction/forward
top level: BBa_I1030
start: 1
end: 19
orientation: inline

intentional junk dna
type: SequenceAnnotation
persistent identity: annotation1877
display id: annotation1877
version: 1
top level: BBa_I1030
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I1030/annotation1877/range1877/1

https://synbiohub.org/public/igem/BBa_I1030/annotation1877/range1877/1
type: Range
persistent identity: range1877
display id: range1877
version: 1
direction: direction/forward
top level: BBa_I1030
start: 55
end: 74
orientation: inline

cI
type: SequenceAnnotation
persistent identity: annotation1866
display id: annotation1866
version: 1
top level: BBa_I1030
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_I1030/annotation1866/range1866/1

https://synbiohub.org/public/igem/BBa_I1030/annotation1866/range1866/1
type: Range
persistent identity: range1866
display id: range1866
version: 1
direction: direction/forward
top level: BBa_I1030
start: 91
end: 840
orientation: inline

TetR
type: SequenceAnnotation
persistent identity: annotation1873
display id: annotation1873
version: 1
top level: BBa_I1030
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I1030/annotation1873/range1873/1

https://synbiohub.org/public/igem/BBa_I1030/annotation1873/range1873/1
type: Range
persistent identity: range1873
display id: range1873
version: 1
direction: direction/forward
top level: BBa_I1030
start: 1
end: 54
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation1879
display id: annotation1879
version: 1
top level: BBa_I1030
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_I1030/annotation1879/range1879/1

https://synbiohub.org/public/igem/BBa_I1030/annotation1879/range1879/1
type: Range
persistent identity: range1879
display id: range1879
version: 1
direction: direction/forward
top level: BBa_I1030
start: 77
end: 82
orientation: inline

BBa_I1030
type: SequenceAnnotation
persistent identity: annotation7050
display id: annotation7050
version: 1
top level: BBa_I1030
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_I1030/annotation7050/range7050/1

https://synbiohub.org/public/igem/BBa_I1030/annotation7050/range7050/1
type: Range
persistent identity: range7050
display id: range7050
version: 1
direction: direction/forward
top level: BBa_I1030
start: 1
end: 840
orientation: inline

2
type: SequenceAnnotation
persistent identity: annotation1875
display id: annotation1875
version: 1
top level: BBa_I1030
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_I1030/annotation1875/range1875/1

https://synbiohub.org/public/igem/BBa_I1030/annotation1875/range1875/1
type: Range
persistent identity: range1875
display id: range1875
version: 1
direction: direction/forward
top level: BBa_I1030
start: 835
end: 840
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1870
display id: annotation1870
version: 1
top level: BBa_I1030
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I1030/annotation1870/range1870/1

https://synbiohub.org/public/igem/BBa_I1030/annotation1870/range1870/1
type: Range
persistent identity: range1870
display id: range1870
version: 1
direction: direction/forward
top level: BBa_I1030
start: 20
end: 25
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation1878
display id: annotation1878
version: 1
top level: BBa_I1030
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_I1030/annotation1878/range1878/1

https://synbiohub.org/public/igem/BBa_I1030/annotation1878/range1878/1
type: Range
persistent identity: range1878
display id: range1878
version: 1
direction: direction/forward
top level: BBa_I1030
start: 55
end: 55
orientation: inline

SsrA
type: SequenceAnnotation
persistent identity: annotation1874
display id: annotation1874
version: 1
top level: BBa_I1030
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_I1030/annotation1874/range1874/1

https://synbiohub.org/public/igem/BBa_I1030/annotation1874/range1874/1
type: Range
persistent identity: range1874
display id: range1874
version: 1
direction: direction/forward
top level: BBa_I1030
start: 802
end: 840
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation1867
display id: annotation1867
version: 1
top level: BBa_I1030
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_I1030/annotation1867/range1867/1

https://synbiohub.org/public/igem/BBa_I1030/annotation1867/range1867/1
type: Range
persistent identity: range1867
display id: range1867
version: 1
direction: direction/forward
top level: BBa_I1030
start: 91
end: 93
orientation: inline

BBa_I1021
type: ComponentDefinition
persistent identity: BBa I1021
display id: BBa_I1021
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Antisense RNA w/BBa_I1020 Interference (KISS)
creator: June Rhee, Connie Tao, Ty Thomson, Louis Waldman
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:29Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Sequence for antisense RNA that interferes with BBa_I1020 mRNA using the KISS scheme.
mutable notes: <bb_part>BBa_I1021</bb_part> antisense RNA is designed to interfere with <bb_part>BBa_I1020</bb_part> mRNA with no expected secondary structure interactions (dubbed the KISS scheme for Keep is Simple Samantha). The sequence is designed to interfere exclusively with <bb_part>BBa_I1020</bb_part>, and act independently of <bb_part>BBa_I1010</bb_part> and <bb_part>BBa_I1011</bb_part>. These parts are designed to use codon space to code for the same protein (<bb_part>BBa_I1010</bb_part> and <bb_part>BBa_1020</bb_part> code for lambda cI) while allowing for independent anti-sense regulation of the <bb_part>BBa_I1010</bb_part> and <bb_part>BBa_1020</bb_part> transcripts via <bb_part>BBa_I1011</bb_part> and <bb_part>BBa_1021</bb_part>
mutable provenance: Anti-sense secondary structure adapted from: Mizuno, T., Proc. Natl. Acad. Sci. USA (1984) 81, 1966-1970
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000834
role: partType/RNA
sequence annotation: cI mRNA reverse complement
sequence annotation: reverse complement
sequence annotation: BBa_I1021
sequence annotation: start
sequence annotation: suffix
sequence annotation: cI CDS Reverse Complement
sequence: https://synbiohub.org/public/igem/BBa_I1021_sequence/1

cI mRNA reverse complement
type: SequenceAnnotation
persistent identity: annotation1838
display id: annotation1838
version: 1
top level: BBa_I1021
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I1021/annotation1838/range1838/1

https://synbiohub.org/public/igem/BBa_I1021/annotation1838/range1838/1
type: Range
persistent identity: range1838
display id: range1838
version: 1
direction: direction/forward
top level: BBa_I1021
start: 1
end: 100
orientation: inline

reverse complement
type: SequenceAnnotation
persistent identity: annotation1840
display id: annotation1840
version: 1
top level: BBa_I1021
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_I1021/annotation1840/range1840/1

https://synbiohub.org/public/igem/BBa_I1021/annotation1840/range1840/1
type: Range
persistent identity: range1840
display id: range1840
version: 1
direction: direction/reverse
top level: BBa_I1021
start: 78
end: 83
orientation: inline

BBa_I1021
type: SequenceAnnotation
persistent identity: annotation7047
display id: annotation7047
version: 1
top level: BBa_I1021
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_I1021/annotation7047/range7047/1

https://synbiohub.org/public/igem/BBa_I1021/annotation7047/range7047/1
type: Range
persistent identity: range7047
display id: range7047
version: 1
direction: direction/forward
top level: BBa_I1021
start: 1
end: 104
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation1837
display id: annotation1837
version: 1
top level: BBa_I1021
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_I1021/annotation1837/range1837/1

https://synbiohub.org/public/igem/BBa_I1021/annotation1837/range1837/1
type: Range
persistent identity: range1837
display id: range1837
version: 1
direction: direction/forward
top level: BBa_I1021
start: 71
end: 73
orientation: inline

suffix
type: SequenceAnnotation
persistent identity: annotation1836
display id: annotation1836
version: 1
top level: BBa_I1021
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I1021/annotation1836/range1836/1

https://synbiohub.org/public/igem/BBa_I1021/annotation1836/range1836/1
type: Range
persistent identity: range1836
display id: range1836
version: 1
direction: direction/forward
top level: BBa_I1021
start: 101
end: 104
orientation: inline

cI CDS Reverse Complement
type: SequenceAnnotation
persistent identity: annotation1839
display id: annotation1839
version: 1
top level: BBa_I1021
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I1021/annotation1839/range1839/1

https://synbiohub.org/public/igem/BBa_I1021/annotation1839/range1839/1
type: Range
persistent identity: range1839
display id: range1839
version: 1
direction: direction/forward
top level: BBa_I1021
start: 1
end: 73
orientation: inline

BBa_I1063
type: ComponentDefinition
persistent identity: BBa I1063
display id: BBa_I1063
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CI(2) IS10 asRNA
creator: Grace Kenney, Daniel Shen, Neelaksh Varshney, Samantha Sutton
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:30Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Region which serves as basis for transcription of asRNA that binds to and inhibits <bb_part>BBa_I1060</bb_part> mRNA transcript. Part of the XOR gate comprised of <bb_part>BBa_I1010</bb_part> and <bb_part>BBa_I1060</bb_part>, their corresponding asRNA coding sequences (<bb_part>BBa_I1013</bb_part> and <bb_part>BBa_I1063</bb_part>).
mutable notes: Complementary to beginning of <bb_part>BBa_I1060</bb_part> transcript covering 5' region, RBS, start codon, and 5 bp into coding sequence. Secondary structure designed for the IS10 anti-sense mRNA mechanism (See below). Can be used with all parts of the BBa_I system. It inhibits <bb_part>BBa_I1060</bb_part>. Incompatible with systems containing cI (wild type)
mutable provenance: see referencers
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000834
role: partType/RNA
sequence annotation: swap
sequence annotation: start
sequence annotation: -35
sequence annotation: TetR 1
sequence annotation: Binds first 2 bf of I1060, I1020
sequence annotation: -10
sequence annotation: stem_loop
sequence annotation: TetR
sequence annotation: HH117a swap
sequence annotation: BBa_I1063
sequence annotation: TetR 2
sequence annotation: RBS Reverse Complement
sequence annotation: C added
sequence: https://synbiohub.org/public/igem/BBa_I1063_sequence/1

swap
type: SequenceAnnotation
persistent identity: annotation1976
display id: annotation1976
version: 1
top level: BBa_I1063
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I1063/annotation1976/range1976/1

https://synbiohub.org/public/igem/BBa_I1063/annotation1976/range1976/1
type: Range
persistent identity: range1976
display id: range1976
version: 1
direction: direction/forward
top level: BBa_I1063
start: 110
end: 110
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation1966
display id: annotation1966
version: 1
top level: BBa_I1063
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_I1063/annotation1966/range1966/1

https://synbiohub.org/public/igem/BBa_I1063/annotation1966/range1966/1
type: Range
persistent identity: range1966
display id: range1966
version: 1
direction: direction/reverse
top level: BBa_I1063
start: 60
end: 62
orientation: inline

swap
type: SequenceAnnotation
persistent identity: annotation1975
display id: annotation1975
version: 1
top level: BBa_I1063
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I1063/annotation1975/range1975/1

https://synbiohub.org/public/igem/BBa_I1063/annotation1975/range1975/1
type: Range
persistent identity: range1975
display id: range1975
version: 1
direction: direction/forward
top level: BBa_I1063
start: 103
end: 103
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1981
display id: annotation1981
version: 1
top level: BBa_I1063
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I1063/annotation1981/range1981/1

https://synbiohub.org/public/igem/BBa_I1063/annotation1981/range1981/1
type: Range
persistent identity: range1981
display id: range1981
version: 1
direction: direction/forward
top level: BBa_I1063
start: 20
end: 25
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation1979
display id: annotation1979
version: 1
top level: BBa_I1063
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_I1063/annotation1979/range1979/1

https://synbiohub.org/public/igem/BBa_I1063/annotation1979/range1979/1
type: Range
persistent identity: range1979
display id: range1979
version: 1
direction: direction/forward
top level: BBa_I1063
start: 55
end: 55
orientation: inline

TetR 1
type: SequenceAnnotation
persistent identity: annotation1980
display id: annotation1980
version: 1
top level: BBa_I1063
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I1063/annotation1980/range1980/1

https://synbiohub.org/public/igem/BBa_I1063/annotation1980/range1980/1
type: Range
persistent identity: range1980
display id: range1980
version: 1
direction: direction/forward
top level: BBa_I1063
start: 1
end: 19
orientation: inline

Binds first 2 bf of I1060, I1020
type: SequenceAnnotation
persistent identity: annotation1969
display id: annotation1969
version: 1
top level: BBa_I1063
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I1063/annotation1969/range1969/1

https://synbiohub.org/public/igem/BBa_I1063/annotation1969/range1969/1
type: Range
persistent identity: range1969
display id: range1969
version: 1
direction: direction/forward
top level: BBa_I1063
start: 55
end: 56
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1983
display id: annotation1983
version: 1
top level: BBa_I1063
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I1063/annotation1983/range1983/1

https://synbiohub.org/public/igem/BBa_I1063/annotation1983/range1983/1
type: Range
persistent identity: range1983
display id: range1983
version: 1
direction: direction/forward
top level: BBa_I1063
start: 43
end: 48
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation1967
display id: annotation1967
version: 1
top level: BBa_I1063
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_I1063/annotation1967/range1967/1

https://synbiohub.org/public/igem/BBa_I1063/annotation1967/range1967/1
type: Range
persistent identity: range1967
display id: range1967
version: 1
direction: direction/forward
top level: BBa_I1063
start: 60
end: 116
orientation: inline

swap
type: SequenceAnnotation
persistent identity: annotation1977
display id: annotation1977
version: 1
top level: BBa_I1063
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I1063/annotation1977/range1977/1

https://synbiohub.org/public/igem/BBa_I1063/annotation1977/range1977/1
type: Range
persistent identity: range1977
display id: range1977
version: 1
direction: direction/forward
top level: BBa_I1063
start: 112
end: 112
orientation: inline

TetR
type: SequenceAnnotation
persistent identity: annotation1984
display id: annotation1984
version: 1
top level: BBa_I1063
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I1063/annotation1984/range1984/1

https://synbiohub.org/public/igem/BBa_I1063/annotation1984/range1984/1
type: Range
persistent identity: range1984
display id: range1984
version: 1
direction: direction/forward
top level: BBa_I1063
start: 1
end: 54
orientation: inline

HH117a swap
type: SequenceAnnotation
persistent identity: annotation1974
display id: annotation1974
version: 1
top level: BBa_I1063
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I1063/annotation1974/range1974/1

https://synbiohub.org/public/igem/BBa_I1063/annotation1974/range1974/1
type: Range
persistent identity: range1974
display id: range1974
version: 1
direction: direction/forward
top level: BBa_I1063
start: 87
end: 87
orientation: inline

BBa_I1063
type: SequenceAnnotation
persistent identity: annotation7061
display id: annotation7061
version: 1
top level: BBa_I1063
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_I1063/annotation7061/range7061/1

https://synbiohub.org/public/igem/BBa_I1063/annotation7061/range7061/1
type: Range
persistent identity: range7061
display id: range7061
version: 1
direction: direction/forward
top level: BBa_I1063
start: 1
end: 169
orientation: inline

swap
type: SequenceAnnotation
persistent identity: annotation1971
display id: annotation1971
version: 1
top level: BBa_I1063
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I1063/annotation1971/range1971/1

https://synbiohub.org/public/igem/BBa_I1063/annotation1971/range1971/1
type: Range
persistent identity: range1971
display id: range1971
version: 1
direction: direction/forward
top level: BBa_I1063
start: 64
end: 64
orientation: inline

swap
type: SequenceAnnotation
persistent identity: annotation1973
display id: annotation1973
version: 1
top level: BBa_I1063
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I1063/annotation1973/range1973/1

https://synbiohub.org/public/igem/BBa_I1063/annotation1973/range1973/1
type: Range
persistent identity: range1973
display id: range1973
version: 1
direction: direction/forward
top level: BBa_I1063
start: 73
end: 73
orientation: inline

TetR 2
type: SequenceAnnotation
persistent identity: annotation1982
display id: annotation1982
version: 1
top level: BBa_I1063
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I1063/annotation1982/range1982/1

https://synbiohub.org/public/igem/BBa_I1063/annotation1982/range1982/1
type: Range
persistent identity: range1982
display id: range1982
version: 1
direction: direction/forward
top level: BBa_I1063
start: 26
end: 44
orientation: inline

swap
type: SequenceAnnotation
persistent identity: annotation1978
display id: annotation1978
version: 1
top level: BBa_I1063
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I1063/annotation1978/range1978/1

https://synbiohub.org/public/igem/BBa_I1063/annotation1978/range1978/1
type: Range
persistent identity: range1978
display id: range1978
version: 1
direction: direction/forward
top level: BBa_I1063
start: 113
end: 113
orientation: inline

RBS Reverse Complement
type: SequenceAnnotation
persistent identity: annotation1964
display id: annotation1964
version: 1
top level: BBa_I1063
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I1063/annotation1964/range1964/1

https://synbiohub.org/public/igem/BBa_I1063/annotation1964/range1964/1
type: Range
persistent identity: range1964
display id: range1964
version: 1
direction: direction/forward
top level: BBa_I1063
start: 67
end: 72
orientation: inline

C added
type: SequenceAnnotation
persistent identity: annotation1968
display id: annotation1968
version: 1
top level: BBa_I1063
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_I1063/annotation1968/range1968/1

https://synbiohub.org/public/igem/BBa_I1063/annotation1968/range1968/1
type: Range
persistent identity: range1968
display id: range1968
version: 1
direction: direction/forward
top level: BBa_I1063
start: 57
end: 59
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation1962
display id: annotation1962
version: 1
top level: BBa_I1063
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_I1063/annotation1962/range1962/1

https://synbiohub.org/public/igem/BBa_I1063/annotation1962/range1962/1
type: Range
persistent identity: range1962
display id: range1962
version: 1
direction: direction/forward
top level: BBa_I1063
start: 78
end: 98
orientation: inline

swap
type: SequenceAnnotation
persistent identity: annotation1972
display id: annotation1972
version: 1
top level: BBa_I1063
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I1063/annotation1972/range1972/1

https://synbiohub.org/public/igem/BBa_I1063/annotation1972/range1972/1
type: Range
persistent identity: range1972
display id: range1972
version: 1
direction: direction/forward
top level: BBa_I1063
start: 66
end: 66
orientation: inline

swap
type: SequenceAnnotation
persistent identity: annotation1970
display id: annotation1970
version: 1
top level: BBa_I1063
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I1063/annotation1970/range1970/1

https://synbiohub.org/public/igem/BBa_I1063/annotation1970/range1970/1
type: Range
persistent identity: range1970
display id: range1970
version: 1
direction: direction/forward
top level: BBa_I1063
start: 63
end: 63
orientation: inline

BBa_I1042
type: ComponentDefinition
persistent identity: BBa I1042
display id: BBa_I1042
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CI(2) "micRNA" asRNA
creator: Grace Kenney, Daniel Shen, Neelaksh Varshney, Samantha Sutton
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:30Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Region which serves as basis for transcription of asRNA that binds to and inhibits <bb_part>BBa_I1040</bb_part> mRNA transcript. Has tetR regulatory region with promoter. Part of the XOR gate comprised of <bb_part>BBa_I1030</bb_part> and <bb_part>BBa_I1040</bb_part>, their corresponding asRNA coding sequences (<bb_part>BBa_I1032</bb_part> and <bb_part>BBa_I1042</bb_part>).
mutable notes: Complementary to beginning of <bb_part>BBa_I1040</bb_part> transcript covering junk region, RBS, start codon, and 73 bp into coding sequence. Two stem loops flank the antisense region. [KEIL96] Incompatible with systems containing <bb_part>BBa_I1041</bb_part> Compatible with <bb_part>BBa_I1020</bb_part>, <bb_part>BBa_I1021</bb_part>, <bb_part>BBa_I1022</bb_part>, <bb_part>BBa_I1023</bb_part>.
mutable provenance: see references
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000834
role: partType/RNA
sequence annotation: tetR
sequence annotation: stem_loop
sequence annotation: BBa_I1042
sequence annotation: antisense region for I1040
sequence: https://synbiohub.org/public/igem/BBa_I1042_sequence/1

tetR
type: SequenceAnnotation
persistent identity: annotation1928
display id: annotation1928
version: 1
top level: BBa_I1042
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_I1042/annotation1928/range1928/1

https://synbiohub.org/public/igem/BBa_I1042/annotation1928/range1928/1
type: Range
persistent identity: range1928
display id: range1928
version: 1
direction: direction/forward
top level: BBa_I1042
start: 1
end: 54
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation1930
display id: annotation1930
version: 1
top level: BBa_I1042
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_I1042/annotation1930/range1930/1

https://synbiohub.org/public/igem/BBa_I1042/annotation1930/range1930/1
type: Range
persistent identity: range1930
display id: range1930
version: 1
direction: direction/forward
top level: BBa_I1042
start: 220
end: 254
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation1929
display id: annotation1929
version: 1
top level: BBa_I1042
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_I1042/annotation1929/range1929/1

https://synbiohub.org/public/igem/BBa_I1042/annotation1929/range1929/1
type: Range
persistent identity: range1929
display id: range1929
version: 1
direction: direction/forward
top level: BBa_I1042
start: 55
end: 111
orientation: inline

BBa_I1042
type: SequenceAnnotation
persistent identity: annotation7057
display id: annotation7057
version: 1
top level: BBa_I1042
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_I1042/annotation7057/range7057/1

https://synbiohub.org/public/igem/BBa_I1042/annotation7057/range7057/1
type: Range
persistent identity: range7057
display id: range7057
version: 1
direction: direction/forward
top level: BBa_I1042
start: 1
end: 254
orientation: inline

antisense region for I1040
type: SequenceAnnotation
persistent identity: annotation1927
display id: annotation1927
version: 1
top level: BBa_I1042
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I1042/annotation1927/range1927/1

https://synbiohub.org/public/igem/BBa_I1042/annotation1927/range1927/1
type: Range
persistent identity: range1927
display id: range1927
version: 1
direction: direction/forward
top level: BBa_I1042
start: 112
end: 219
orientation: inline

BBa_J04676
type: ComponentDefinition
persistent identity: BBa J04676
display id: BBa_J04676
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: R0010 with Anti-Sense Theophillline Induced Antiswitch ("Off Switch")
creator: Oscar Hernandez and Matt Gemberling
created: 2005-09-14T11:00:00Z
modified: 2015-08-31T04:08:14Z
discontinued: false
dominant: false
group u list: _16_
m user id: 0
owner id: 435
owning group id: 16
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This Promoter and Antiswitch construct is designed so that it can be attached to any form of EYFP or YFP coding region. The purpose of this piece is to regulate the expression of YFP in the presence of theophylline. In this construct, the presence of theophylline causes the antiswitch, which is initially bound up on itself (allowing expression), to release and unfold allowing it to bind to the YFP. This, in turn, halts the expression of YFP.
mutable notes: Adaptation of antiswitch design "S8" by Bayer and Smolke; article available at http://www.nature.com/nbt/journal/v23/n3/full/nbt1069.html
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000834
role: partType/RNA
sequence annotation: Hammerhead Riobozyme #1
sequence annotation: R0010 Promoter Region
sequence annotation: Hammerhead Riobozyme #2
sequence annotation: Antisense region (binds with target)
sequence annotation: Antiswitch
sequence: https://synbiohub.org/public/igem/BBa_J04676_sequence/1

Hammerhead Riobozyme #1
type: SequenceAnnotation
persistent identity: annotation1692475
display id: annotation1692475
version: 1
top level: BBa_J04676
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_J04676/annotation1692475/range1692475/1

https://synbiohub.org/public/igem/BBa_J04676/annotation1692475/range1692475/1
type: Range
persistent identity: range1692475
display id: range1692475
version: 1
direction: direction/forward
top level: BBa_J04676
start: 201
end: 253
orientation: inline

R0010 Promoter Region
type: SequenceAnnotation
persistent identity: annotation1692468
display id: annotation1692468
version: 1
top level: BBa_J04676
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_J04676/annotation1692468/range1692468/1

https://synbiohub.org/public/igem/BBa_J04676/annotation1692468/range1692468/1
type: Range
persistent identity: range1692468
display id: range1692468
version: 1
direction: direction/forward
top level: BBa_J04676
start: 1
end: 200
orientation: inline

Hammerhead Riobozyme #2
type: SequenceAnnotation
persistent identity: annotation1692563
display id: annotation1692563
version: 1
top level: BBa_J04676
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_J04676/annotation1692563/range1692563/1

https://synbiohub.org/public/igem/BBa_J04676/annotation1692563/range1692563/1
type: Range
persistent identity: range1692563
display id: range1692563
version: 1
direction: direction/forward
top level: BBa_J04676
start: 328
end: 378
orientation: inline

Antisense region (binds with target)
type: SequenceAnnotation
persistent identity: annotation1692585
display id: annotation1692585
version: 1
top level: BBa_J04676
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_J04676/annotation1692585/range1692585/1

https://synbiohub.org/public/igem/BBa_J04676/annotation1692585/range1692585/1
type: Range
persistent identity: range1692585
display id: range1692585
version: 1
direction: direction/forward
top level: BBa_J04676
start: 254
end: 267
orientation: inline

Antiswitch
type: SequenceAnnotation
persistent identity: annotation1692541
display id: annotation1692541
version: 1
top level: BBa_J04676
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_J04676/annotation1692541/range1692541/1

https://synbiohub.org/public/igem/BBa_J04676/annotation1692541/range1692541/1
type: Range
persistent identity: range1692541
display id: range1692541
version: 1
direction: direction/forward
top level: BBa_J04676
start: 254
end: 327
orientation: inline