//classic/signalling/receiver
type: Collection
persistent identity: classic signalling receiver
display id: classic_signalling_receiver
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
owned by: james
owned by: myers
member: BBa_F2621
member: BBa_F2790
member: virG
member: Pgad
member: BBa_K546000
member: BBa_K772004
member: BBa_F2710
member: QS->RFP
member: BBa_K546002
member: BBa_F2622
member: BBa_K574009
member: BBa_F2620
member: BBa_F2770
member: BBa_K990001
member: BBa_K574001
member: BBa_K621001
member: BBa_K546001
member: BBa_K546003
member: BBa_K546005

BBa_K574009
type: ComponentDefinition
persistent identity: BBa K574009
display id: BBa_K574009
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: 3OC12HSL -> PoPS Receiver
creator: Lei Wei
created: 2011-09-28T11:00:00Z
modified: 2015-05-08T01:12:44Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _745_
m user id: 0
owner id: 8529
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: PoPS -> LasI
mutable notes: Empty.
mutable provenance: Existing Part.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Signalling
role: SO:0000804
component: BBa_R0079
component: BBa_K574008
sequence annotation: BBa_R0079
sequence annotation: BBa_K574008
sequence: https://synbiohub.org/public/igem/BBa_K574009_sequence/1

BBa_R0079
type: Component
persistent identity: component2264357
display id: component2264357
version: 1
top level: BBa_K574009
access: public
definition: https://synbiohub.org/public/igem/BBa_R0079/1

BBa_K574008
type: Component
persistent identity: component2264352
display id: component2264352
version: 1
top level: BBa_K574009
access: public
definition: https://synbiohub.org/public/igem/BBa_K574008/1

BBa_R0079
type: SequenceAnnotation
persistent identity: annotation2264357
display id: annotation2264357
version: 1
top level: BBa_K574009
location: https://synbiohub.org/public/igem/BBa_K574009/annotation2264357/range2264357/1
component: BBa_R0079

https://synbiohub.org/public/igem/BBa_K574009/annotation2264357/range2264357/1
type: Range
persistent identity: range2264357
display id: range2264357
version: 1
direction: direction/reverse
top level: BBa_K574009
start: 991
end: 1147
orientation: inline

BBa_K574008
type: SequenceAnnotation
persistent identity: annotation2264352
display id: annotation2264352
version: 1
top level: BBa_K574009
location: https://synbiohub.org/public/igem/BBa_K574009/annotation2264352/range2264352/1
component: BBa_K574008

https://synbiohub.org/public/igem/BBa_K574009/annotation2264352/range2264352/1
type: Range
persistent identity: range2264352
display id: range2264352
version: 1
direction: direction/reverse
top level: BBa_K574009
start: 1
end: 982
orientation: inline

BBa_F2622
type: ComponentDefinition
persistent identity: BBa F2622
display id: BBa_F2622
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: 3OC6HSL Receiver Device
creator: Barry Canton
created: 2004-12-14T12:00:00Z
modified: 2015-08-31T04:07:27Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _11_6_
m user id: 0
owner id: 135
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This device senses the 3OC6HSL concentration in the media and produces output PoPS according to the transfer function given below. LuxR production is controlled by the lac repressible R0011.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Signalling
role: SO:0000804
component: BBa_B0012
component: BBa_B0034
component: BBa_C0062
component: BBa_R0011
component: BBa_B0010
component: BBa_R0062
sequence annotation: BBa_B0012
sequence annotation: BBa_B0034
sequence annotation: BBa_B0010
sequence annotation: BBa_R0011
sequence annotation: BBa_R0062
sequence annotation: BBa_C0062
sequence: https://synbiohub.org/public/igem/BBa_F2622_sequence/1

BBa_B0012
type: Component
persistent identity: component1484681
display id: component1484681
version: 1
top level: BBa_F2622
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0034
type: Component
persistent identity: component1484648
display id: component1484648
version: 1
top level: BBa_F2622
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_C0062
type: Component
persistent identity: component1484663
display id: component1484663
version: 1
top level: BBa_F2622
access: public
definition: https://synbiohub.org/public/igem/BBa_C0062/1

BBa_R0011
type: Component
persistent identity: component1484639
display id: component1484639
version: 1
top level: BBa_F2622
access: public
definition: https://synbiohub.org/public/igem/BBa_R0011/1

BBa_B0010
type: Component
persistent identity: component1484671
display id: component1484671
version: 1
top level: BBa_F2622
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_R0062
type: Component
persistent identity: component1484696
display id: component1484696
version: 1
top level: BBa_F2622
access: public
definition: https://synbiohub.org/public/igem/BBa_R0062/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1484681
display id: annotation1484681
version: 1
top level: BBa_F2622
location: https://synbiohub.org/public/igem/BBa_F2622/annotation1484681/range1484681/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_F2622/annotation1484681/range1484681/1
type: Range
persistent identity: range1484681
display id: range1484681
version: 1
direction: direction/forward
top level: BBa_F2622
start: 959
end: 999
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1484648
display id: annotation1484648
version: 1
top level: BBa_F2622
location: https://synbiohub.org/public/igem/BBa_F2622/annotation1484648/range1484648/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_F2622/annotation1484648/range1484648/1
type: Range
persistent identity: range1484648
display id: range1484648
version: 1
direction: direction/forward
top level: BBa_F2622
start: 64
end: 75
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1484671
display id: annotation1484671
version: 1
top level: BBa_F2622
location: https://synbiohub.org/public/igem/BBa_F2622/annotation1484671/range1484671/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_F2622/annotation1484671/range1484671/1
type: Range
persistent identity: range1484671
display id: range1484671
version: 1
direction: direction/forward
top level: BBa_F2622
start: 871
end: 950
orientation: inline

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation1484639
display id: annotation1484639
version: 1
top level: BBa_F2622
location: https://synbiohub.org/public/igem/BBa_F2622/annotation1484639/range1484639/1
component: BBa_R0011

https://synbiohub.org/public/igem/BBa_F2622/annotation1484639/range1484639/1
type: Range
persistent identity: range1484639
display id: range1484639
version: 1
direction: direction/forward
top level: BBa_F2622
start: 1
end: 54
orientation: inline

BBa_R0062
type: SequenceAnnotation
persistent identity: annotation1484696
display id: annotation1484696
version: 1
top level: BBa_F2622
location: https://synbiohub.org/public/igem/BBa_F2622/annotation1484696/range1484696/1
component: BBa_R0062

https://synbiohub.org/public/igem/BBa_F2622/annotation1484696/range1484696/1
type: Range
persistent identity: range1484696
display id: range1484696
version: 1
direction: direction/forward
top level: BBa_F2622
start: 1008
end: 1062
orientation: inline

BBa_C0062
type: SequenceAnnotation
persistent identity: annotation1484663
display id: annotation1484663
version: 1
top level: BBa_F2622
location: https://synbiohub.org/public/igem/BBa_F2622/annotation1484663/range1484663/1
component: BBa_C0062

https://synbiohub.org/public/igem/BBa_F2622/annotation1484663/range1484663/1
type: Range
persistent identity: range1484663
display id: range1484663
version: 1
direction: direction/forward
top level: BBa_F2622
start: 82
end: 837
orientation: inline

BBa_K546003
type: ComponentDefinition
persistent identity: BBa K546003
display id: BBa_K546003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Lux pL controlled LuxR + lux pR promoter.
creator: Brendan Ryback, Matthijn Hesselman
created: 2011-09-13T11:00:00Z
modified: 2015-05-08T01:12:39Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _713_
m user id: 0
owner id: 8616
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This part was made as a replacement for a defective version of BBa_F2621 which lacked the R0063 promoter, but was otherwise intact and functional. It shows a significantly higher response to 3OC6HSL than the incomplete BBa_F2621 (which is present in the Registry as BBa_K317037)
mutable notes: No specific considerations.
mutable provenance: Was constructed by cloning BBa_R0063 in front of the incomplete BBa_F2621 (sent from the Registry in July 2011 lacking the R0063 subcomponent)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Signalling
role: SO:0000804
component: BBa_K317037
component: BBa_R0063
sequence annotation: BBa_K317037
sequence annotation: BBa_R0063
sequence: https://synbiohub.org/public/igem/BBa_K546003_sequence/1

BBa_K317037
type: Component
persistent identity: component2264075
display id: component2264075
version: 1
top level: BBa_K546003
access: public
definition: https://synbiohub.org/public/igem/BBa_K317037/1

BBa_R0063
type: Component
persistent identity: component2264050
display id: component2264050
version: 1
top level: BBa_K546003
access: public
definition: https://synbiohub.org/public/igem/BBa_R0063/1

BBa_K317037
type: SequenceAnnotation
persistent identity: annotation2264075
display id: annotation2264075
version: 1
top level: BBa_K546003
location: https://synbiohub.org/public/igem/BBa_K546003/annotation2264075/range2264075/1
component: BBa_K317037

https://synbiohub.org/public/igem/BBa_K546003/annotation2264075/range2264075/1
type: Range
persistent identity: range2264075
display id: range2264075
version: 1
direction: direction/reverse
top level: BBa_K546003
start: 160
end: 1158
orientation: inline

BBa_R0063
type: SequenceAnnotation
persistent identity: annotation2264050
display id: annotation2264050
version: 1
top level: BBa_K546003
location: https://synbiohub.org/public/igem/BBa_K546003/annotation2264050/range2264050/1
component: BBa_R0063

https://synbiohub.org/public/igem/BBa_K546003/annotation2264050/range2264050/1
type: Range
persistent identity: range2264050
display id: range2264050
version: 1
direction: direction/forward
top level: BBa_K546003
start: 1
end: 151
orientation: inline

Pgad
type: ComponentDefinition
persistent identity: BBa K559011
display id: BBa_K559011
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Pgad - Intracellular Chloride-Sensing Cassette
creator: Jacky, Fong Chuen, Loo
created: 2011-10-27T11:00:00Z
modified: 2015-05-08T01:12:41Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _727_
m user id: 0
owner id: 6123
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: Pgad is chloride-sensitive promoter which was first discovered in Lactococcus lactis1, which is a gram-positive bacterium which can live in acidic environment. In this operon, gadC is glutamate-gamma-aminobutyrate antiporter and gadB is glutamate decarboxylase. They are both involved in intracellular pH regulation and co-expressed in the same operon under the control of Pgad2. The gene before Pgad, named gadR, which is constitutively expressed under the control of PgadR, is a positive regulator of Pgad coupled genes while intracellular chloride is level elevated.
mutable notes: Synthesized part by GenScript,all the illegal sites for RFC 10 (EcoRI, XbaI, SpeI, PstI) have been substituted by other codon producing the same amino acid in E. coli.
mutable provenance: The gene sequence was obtained from the cassette from bp 821 to 2071 of GenBank sequence AF005098, which includes PgadR, gadR, Pgad and the starting codon ATG.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: Pgad
sequence annotation: RBS_gadR
sequence annotation: RBS_gadC
sequence annotation: gadR
sequence annotation: gadC Start
sequence annotation: PgadR
sequence: https://synbiohub.org/public/igem/BBa_K559011_sequence/1

Pgad
type: SequenceAnnotation
persistent identity: annotation2160939
display id: annotation2160939
version: 1
top level: Pgad
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K559011/annotation2160939/range2160939/1

https://synbiohub.org/public/igem/BBa_K559011/annotation2160939/range2160939/1
type: Range
persistent identity: range2160939
display id: range2160939
version: 1
direction: direction/forward
top level: Pgad
start: 1163
end: 1181
orientation: inline

RBS_gadR
type: SequenceAnnotation
persistent identity: annotation2160941
display id: annotation2160941
version: 1
top level: Pgad
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K559011/annotation2160941/range2160941/1

https://synbiohub.org/public/igem/BBa_K559011/annotation2160941/range2160941/1
type: Range
persistent identity: range2160941
display id: range2160941
version: 1
direction: direction/forward
top level: Pgad
start: 264
end: 269
orientation: inline

RBS_gadC
type: SequenceAnnotation
persistent identity: annotation2160940
display id: annotation2160940
version: 1
top level: Pgad
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K559011/annotation2160940/range2160940/1

https://synbiohub.org/public/igem/BBa_K559011/annotation2160940/range2160940/1
type: Range
persistent identity: range2160940
display id: range2160940
version: 1
direction: direction/forward
top level: Pgad
start: 1237
end: 1245
orientation: inline

gadR
type: SequenceAnnotation
persistent identity: annotation2160938
display id: annotation2160938
version: 1
top level: Pgad
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K559011/annotation2160938/range2160938/1

https://synbiohub.org/public/igem/BBa_K559011/annotation2160938/range2160938/1
type: Range
persistent identity: range2160938
display id: range2160938
version: 1
direction: direction/forward
top level: Pgad
start: 275
end: 1105
orientation: inline

gadC Start
type: SequenceAnnotation
persistent identity: annotation2160936
display id: annotation2160936
version: 1
top level: Pgad
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K559011/annotation2160936/range2160936/1

https://synbiohub.org/public/igem/BBa_K559011/annotation2160936/range2160936/1
type: Range
persistent identity: range2160936
display id: range2160936
version: 1
direction: direction/forward
top level: Pgad
start: 1249
end: 1251
orientation: inline

PgadR
type: SequenceAnnotation
persistent identity: annotation2160937
display id: annotation2160937
version: 1
top level: Pgad
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K559011/annotation2160937/range2160937/1

https://synbiohub.org/public/igem/BBa_K559011/annotation2160937/range2160937/1
type: Range
persistent identity: range2160937
display id: range2160937
version: 1
direction: direction/forward
top level: Pgad
start: 141
end: 158
orientation: inline

virG
type: ComponentDefinition
persistent identity: BBa K389002
display id: BBa_K389002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Mutated virG
creator: Jonas Aretz
created: 2010-08-09T11:00:00Z
modified: 2015-05-08T01:12:18Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _517_
m user id: 0
owner id: 6670
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This is the ''virG'' response regulator from the virA/G receptor system. The virA/G receptor system is used by ''Agrobacterium tumefaciens'' to detect phenolic substances secreted by plants. In presence of these substances, ''virG'' is activated by the ''virA'' receptor and induces the transcription of genes under the control of a ''vir'' promotor. Normally, ''virG'' needs the ''rpoA'' RNA polymerase subunit from ''A. tumefaciens'' to work in ''Escherichia coli'' but this ''virG'' BioBrick is mutated, so works with the ''rpoA'' subunit from ''E. coli''.
mutable notes: synthesized by Mr. Gene, so optimated codon usage, removal of illegal restriction sites
mutable provenance: synthetic
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Stop codon
sequence annotation: virG
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_K389002_sequence/1

Stop codon
type: SequenceAnnotation
persistent identity: annotation2078362
display id: annotation2078362
version: 1
top level: virG
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K389002/annotation2078362/range2078362/1

https://synbiohub.org/public/igem/BBa_K389002/annotation2078362/range2078362/1
type: Range
persistent identity: range2078362
display id: range2078362
version: 1
direction: direction/forward
top level: virG
start: 738
end: 740
orientation: inline

virG
type: SequenceAnnotation
persistent identity: annotation2078361
display id: annotation2078361
version: 1
top level: virG
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K389002/annotation2078361/range2078361/1

https://synbiohub.org/public/igem/BBa_K389002/annotation2078361/range2078361/1
type: Range
persistent identity: range2078361
display id: range2078361
version: 1
direction: direction/forward
top level: virG
start: 12
end: 737
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2078360
display id: annotation2078360
version: 1
top level: virG
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K389002/annotation2078360/range2078360/1

https://synbiohub.org/public/igem/BBa_K389002/annotation2078360/range2078360/1
type: Range
persistent identity: range2078360
display id: range2078360
version: 1
direction: direction/forward
top level: virG
start: 4
end: 8
orientation: inline

BBa_F2621
type: ComponentDefinition
persistent identity: BBa F2621
display id: BBa_F2621
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: 3OC6HSL Receiver Device
creator: Barry Canton
created: 2004-08-08T11:00:00Z
modified: 2015-08-31T04:07:27Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _11_6_
m user id: 0
owner id: 135
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This device senses the 3OC6HSL concentration in the media and produces output PoPS according to the transfer function given below. LuxR production is controlled by the LuxR controllable R0063.
mutable provenance: Formerly I13260
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Signalling
role: SO:0000804
component: BBa_R0063
component: BBa_B0012
component: BBa_R0062
component: BBa_B0010
component: BBa_B0034
component: BBa_C0062
sequence annotation: BBa_B0010
sequence annotation: BBa_B0034
sequence annotation: BBa_R0063
sequence annotation: BBa_R0062
sequence annotation: BBa_C0062
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_F2621_sequence/1

BBa_R0063
type: Component
persistent identity: component1484730
display id: component1484730
version: 1
top level: BBa_F2621
access: public
definition: https://synbiohub.org/public/igem/BBa_R0063/1

BBa_B0012
type: Component
persistent identity: component1484772
display id: component1484772
version: 1
top level: BBa_F2621
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_R0062
type: Component
persistent identity: component1484787
display id: component1484787
version: 1
top level: BBa_F2621
access: public
definition: https://synbiohub.org/public/igem/BBa_R0062/1

BBa_B0010
type: Component
persistent identity: component1484762
display id: component1484762
version: 1
top level: BBa_F2621
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0034
type: Component
persistent identity: component1484739
display id: component1484739
version: 1
top level: BBa_F2621
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_C0062
type: Component
persistent identity: component1484754
display id: component1484754
version: 1
top level: BBa_F2621
access: public
definition: https://synbiohub.org/public/igem/BBa_C0062/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1484762
display id: annotation1484762
version: 1
top level: BBa_F2621
location: https://synbiohub.org/public/igem/BBa_F2621/annotation1484762/range1484762/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_F2621/annotation1484762/range1484762/1
type: Range
persistent identity: range1484762
display id: range1484762
version: 1
direction: direction/forward
top level: BBa_F2621
start: 967
end: 1046
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1484739
display id: annotation1484739
version: 1
top level: BBa_F2621
location: https://synbiohub.org/public/igem/BBa_F2621/annotation1484739/range1484739/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_F2621/annotation1484739/range1484739/1
type: Range
persistent identity: range1484739
display id: range1484739
version: 1
direction: direction/forward
top level: BBa_F2621
start: 160
end: 171
orientation: inline

BBa_R0063
type: SequenceAnnotation
persistent identity: annotation1484730
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component: BBa_R0062

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persistent identity: annotation1484754
display id: annotation1484754
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BBa_B0012
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display id: annotation1484772
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component: BBa_B0012

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BBa_K546005
type: ComponentDefinition
persistent identity: BBa K546005
display id: BBa_K546005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Lux pL controlled LuxR + lux pR autoinducing LuxI (lva tag) + lux pR controlled GFP(lva tag).
creator: Matthijn Hesselman
created: 2011-09-17T11:00:00Z
modified: 2015-05-08T01:12:39Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _713_
m user id: 0
owner id: 8603
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: in progress
mutable notes: in progress
mutable provenance: in progress
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_C0062
component: BBa_B0010
component: BBa_B0012
component: BBa_R0063
component: BBa_B0034
component: BBa_R0062
component: BBa_J04031
component: BBa_C0061
sequence annotation: BBa_B0010
sequence annotation: BBa_B0034
sequence annotation: BBa_R0063
sequence annotation: BBa_B0012
sequence annotation: BBa_C0061
sequence annotation: BBa_C0062
sequence annotation: BBa_R0062
sequence annotation: BBa_J04031
sequence: https://synbiohub.org/public/igem/BBa_K546005_sequence/1

BBa_C0062
type: Component
persistent identity: component2320974
display id: component2320974
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access: public
definition: https://synbiohub.org/public/igem/BBa_C0062/1

BBa_B0010
type: Component
persistent identity: component2320940
display id: component2320940
version: 1
top level: BBa_K546005
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0012
type: Component
persistent identity: component2320997
display id: component2320997
version: 1
top level: BBa_K546005
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_R0063
type: Component
persistent identity: component2320965
display id: component2320965
version: 1
top level: BBa_K546005
access: public
definition: https://synbiohub.org/public/igem/BBa_R0063/1

BBa_B0034
type: Component
persistent identity: component2320971
display id: component2320971
version: 1
top level: BBa_K546005
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0034
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display id: component2320933
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access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_R0062
type: Component
persistent identity: component2320947
display id: component2320947
version: 1
top level: BBa_K546005
access: public
definition: https://synbiohub.org/public/igem/BBa_R0062/1

BBa_B0012
type: Component
persistent identity: component2320942
display id: component2320942
version: 1
top level: BBa_K546005
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0010
type: Component
persistent identity: component2320978
display id: component2320978
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access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_R0063
type: Component
persistent identity: component2320927
display id: component2320927
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definition: https://synbiohub.org/public/igem/BBa_R0063/1

BBa_B0010
type: Component
persistent identity: component2320958
display id: component2320958
version: 1
top level: BBa_K546005
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_C0062
type: Component
persistent identity: component2320936
display id: component2320936
version: 1
top level: BBa_K546005
access: public
definition: https://synbiohub.org/public/igem/BBa_C0062/1

BBa_B0010
type: Component
persistent identity: component2320995
display id: component2320995
version: 1
top level: BBa_K546005
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0012
type: Component
persistent identity: component2320960
display id: component2320960
version: 1
top level: BBa_K546005
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_R0062
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persistent identity: component2320985
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top level: BBa_K546005
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definition: https://synbiohub.org/public/igem/BBa_R0062/1

BBa_B0012
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definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0034
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persistent identity: component2320952
display id: component2320952
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top level: BBa_K546005
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definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_J04031
type: Component
persistent identity: component2320957
display id: component2320957
version: 1
top level: BBa_K546005
access: public
definition: https://synbiohub.org/public/igem/BBa_J04031/1

BBa_B0034
type: Component
persistent identity: component2320990
display id: component2320990
version: 1
top level: BBa_K546005
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_C0061
type: Component
persistent identity: component2320992
display id: component2320992
version: 1
top level: BBa_K546005
access: public
definition: https://synbiohub.org/public/igem/BBa_C0061/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2320940
display id: annotation2320940
version: 1
top level: BBa_K546005
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BBa_B0034
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display id: annotation2320971
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orientation: inline

BBa_R0063
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persistent identity: annotation2320965
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BBa_B0012
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persistent identity: annotation2320960
display id: annotation2320960
version: 1
top level: BBa_K546005
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persistent identity: range2320960
display id: range2320960
version: 1
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start: 2040
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BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2320933
display id: annotation2320933
version: 1
top level: BBa_K546005
location: https://synbiohub.org/public/igem/BBa_K546005/annotation2320933/range2320933/1
component: BBa_B0034

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type: Range
persistent identity: range2320933
display id: range2320933
version: 1
direction: direction/reverse
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start: 160
end: 171
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BBa_C0061
type: SequenceAnnotation
persistent identity: annotation2320992
display id: annotation2320992
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component: BBa_C0061

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persistent identity: range2320992
display id: range2320992
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end: 3890
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2320990
display id: annotation2320990
version: 1
top level: BBa_K546005
location: https://synbiohub.org/public/igem/BBa_K546005/annotation2320990/range2320990/1
component: BBa_B0034

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persistent identity: range2320990
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persistent identity: annotation2320974
display id: annotation2320974
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persistent identity: range2320974
display id: range2320974
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BBa_C0062
type: SequenceAnnotation
persistent identity: annotation2320936
display id: annotation2320936
version: 1
top level: BBa_K546005
location: https://synbiohub.org/public/igem/BBa_K546005/annotation2320936/range2320936/1
component: BBa_C0062

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persistent identity: range2320936
display id: range2320936
version: 1
direction: direction/forward
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end: 933
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BBa_R0062
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persistent identity: annotation2320985
display id: annotation2320985
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top level: BBa_K546005
location: https://synbiohub.org/public/igem/BBa_K546005/annotation2320985/range2320985/1
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persistent identity: range2320985
display id: range2320985
version: 1
direction: direction/forward
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start: 3192
end: 3246
orientation: inline

BBa_J04031
type: SequenceAnnotation
persistent identity: annotation2320957
display id: annotation2320957
version: 1
top level: BBa_K546005
location: https://synbiohub.org/public/igem/BBa_K546005/annotation2320957/range2320957/1
component: BBa_J04031

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type: Range
persistent identity: range2320957
display id: range2320957
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direction: direction/reverse
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start: 1185
end: 1943
orientation: inline

BBa_R0062
type: SequenceAnnotation
persistent identity: annotation2320947
display id: annotation2320947
version: 1
top level: BBa_K546005
location: https://synbiohub.org/public/igem/BBa_K546005/annotation2320947/range2320947/1
component: BBa_R0062

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type: Range
persistent identity: range2320947
display id: range2320947
version: 1
direction: direction/forward
top level: BBa_K546005
start: 1104
end: 1158
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2320978
display id: annotation2320978
version: 1
top level: BBa_K546005
location: https://synbiohub.org/public/igem/BBa_K546005/annotation2320978/range2320978/1
component: BBa_B0010

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type: Range
persistent identity: range2320978
display id: range2320978
version: 1
direction: direction/forward
top level: BBa_K546005
start: 3055
end: 3134
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2320980
display id: annotation2320980
version: 1
top level: BBa_K546005
location: https://synbiohub.org/public/igem/BBa_K546005/annotation2320980/range2320980/1
component: BBa_B0012

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type: Range
persistent identity: range2320980
display id: range2320980
version: 1
direction: direction/forward
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start: 3143
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orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2320942
display id: annotation2320942
version: 1
top level: BBa_K546005
location: https://synbiohub.org/public/igem/BBa_K546005/annotation2320942/range2320942/1
component: BBa_B0012

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type: Range
persistent identity: range2320942
display id: range2320942
version: 1
direction: direction/forward
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start: 1055
end: 1095
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2320997
display id: annotation2320997
version: 1
top level: BBa_K546005
location: https://synbiohub.org/public/igem/BBa_K546005/annotation2320997/range2320997/1
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persistent identity: range2320997
display id: range2320997
version: 1
direction: direction/forward
top level: BBa_K546005
start: 4012
end: 4052
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2320995
display id: annotation2320995
version: 1
top level: BBa_K546005
location: https://synbiohub.org/public/igem/BBa_K546005/annotation2320995/range2320995/1
component: BBa_B0010

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type: Range
persistent identity: range2320995
display id: range2320995
version: 1
direction: direction/forward
top level: BBa_K546005
start: 3924
end: 4003
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2320952
display id: annotation2320952
version: 1
top level: BBa_K546005
location: https://synbiohub.org/public/igem/BBa_K546005/annotation2320952/range2320952/1
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persistent identity: range2320952
display id: range2320952
version: 1
direction: direction/reverse
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start: 1167
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BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2320958
display id: annotation2320958
version: 1
top level: BBa_K546005
location: https://synbiohub.org/public/igem/BBa_K546005/annotation2320958/range2320958/1
component: BBa_B0010

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type: Range
persistent identity: range2320958
display id: range2320958
version: 1
direction: direction/forward
top level: BBa_K546005
start: 1952
end: 2031
orientation: inline

QS->RFP
type: ComponentDefinition
persistent identity: BBa J85120
display id: BBa_J85120
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: High Population->mRFP (pLac->LuxI; pLacIQ->luxR; pLux->mRFP;)
creator: Cory Li
created: 2010-05-31T11:00:00Z
modified: 2015-05-08T01:08:29Z
discontinued: false
dominant: false
experience: experience/None
group u list: _406_
m user id: 0
owner id: 2073
owning group id: 387
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Quorum sensing circuit under a pLac promoter. Population turns red (mRFP) once quorum sensing kicks in.
mutable notes: Double terminator not used after LuxI because it is unnecessary and has the potential to make the plasmid more unstable.
mutable provenance: Built through assembly of BBa_K084014 and BBa_J10000.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_J10000
component: BBa_K084014
sequence annotation: BBa_K084014
sequence annotation: BBa_J10000
sequence: https://synbiohub.org/public/igem/BBa_J85120_sequence/1

BBa_J10000
type: Component
persistent identity: component2294832
display id: component2294832
version: 1
top level: QS->RFP
access: public
definition: https://synbiohub.org/public/igem/BBa_J10000/1

BBa_K084014
type: Component
persistent identity: component2294800
display id: component2294800
version: 1
top level: QS->RFP
access: public
definition: https://synbiohub.org/public/igem/BBa_K084014/1

BBa_K084014
type: SequenceAnnotation
persistent identity: annotation2294800
display id: annotation2294800
version: 1
top level: QS->RFP
location: https://synbiohub.org/public/igem/BBa_J85120/annotation2294800/range2294800/1
component: BBa_K084014

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type: Range
persistent identity: range2294800
display id: range2294800
version: 1
direction: direction/reverse
top level: QS->RFP
start: 1
end: 869
orientation: inline

BBa_J10000
type: SequenceAnnotation
persistent identity: annotation2294832
display id: annotation2294832
version: 1
top level: QS->RFP
location: https://synbiohub.org/public/igem/BBa_J85120/annotation2294832/range2294832/1
component: BBa_J10000

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type: Range
persistent identity: range2294832
display id: range2294832
version: 1
direction: direction/reverse
top level: QS->RFP
start: 878
end: 2653
orientation: inline

BBa_K546000
type: ComponentDefinition
persistent identity: BBa K546000
display id: BBa_K546000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Lux pL controlled LuxR with lux pR autoinducing LuxI(lva tag)- AHL.
creator: M. Hesselman
created: 2011-09-13T11:00:00Z
modified: 2015-05-08T01:12:39Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _713_
m user id: 0
owner id: 9055
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: The F2621 BioBrick device contains a lux pL regulated luxR repressor/activator and a lux pR promoter that is linked to an 3OC6HSL, an AHL autoinducer molecule, synthetase. The lux pL promoter yields a weak constitutive downstream expression. When LuxR and 3OC6HSL are produced, the lux pL promoter, however, will be repressed by a complex of the two. Complementary to the lux pL promoter, the lux pR promoter with a complex of luxR and 3OC6HSL will enable enlarged activation of the downstream synthetase gene, thus increase the concentration 3OC6HSL.
mutable notes: The original R0063 BioBrick part (promoter) of the F2621 BioBrick device turned out to be absent in our construct, as was shown by a sequence analysis. To increase the transcription activation there has been put an extra R0063 BioBrick part promoter in front of the F2621 BioBrick device. The resulting construct worked a lot better than the original F2621 - K082014 BioBrick system.
mutable provenance: The lux p promoters and the autoinducer producing enzyme originate from the quorum sensing ability of ''Vibrio fischeri''.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Signalling
role: SO:0000804
component: BBa_K082014
component: BBa_F2621
sequence annotation: BBa_K082014
sequence annotation: BBa_F2621
sequence: https://synbiohub.org/public/igem/BBa_K546000_sequence/1

BBa_K082014
type: Component
persistent identity: component2289564
display id: component2289564
version: 1
top level: BBa_K546000
access: public
definition: https://synbiohub.org/public/igem/BBa_K082014/1

BBa_F2621
type: Component
persistent identity: component2289549
display id: component2289549
version: 1
top level: BBa_K546000
access: public
definition: BBa_F2621

BBa_K082014
type: SequenceAnnotation
persistent identity: annotation2289564
display id: annotation2289564
version: 1
top level: BBa_K546000
location: https://synbiohub.org/public/igem/BBa_K546000/annotation2289564/range2289564/1
component: BBa_K082014

https://synbiohub.org/public/igem/BBa_K546000/annotation2289564/range2289564/1
type: Range
persistent identity: range2289564
display id: range2289564
version: 1
direction: direction/reverse
top level: BBa_K546000
start: 1167
end: 1964
orientation: inline

BBa_F2621
type: SequenceAnnotation
persistent identity: annotation2289549
display id: annotation2289549
version: 1
top level: BBa_K546000
location: https://synbiohub.org/public/igem/BBa_K546000/annotation2289549/range2289549/1
component: BBa_F2621

https://synbiohub.org/public/igem/BBa_K546000/annotation2289549/range2289549/1
type: Range
persistent identity: range2289549
display id: range2289549
version: 1
direction: direction/reverse
top level: BBa_K546000
start: 1
end: 1158
orientation: inline

BBa_F2620
type: ComponentDefinition
persistent identity: BBa F2620
display id: BBa_F2620
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: 3OC6HSL -> PoPS Receiver
creator: Barry Canton [bcanton@mit.edu] and Anna Labno [labnoa@mit.edu]
created: 2004-08-08T11:00:00Z
modified: 2015-08-31T04:07:27Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _101_6_
m user id: 0
owner id: 135
owning group id: 6
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This device senses the 3OC6HSL concentration in the media and produces output PoPS according to the transfer function given below. LuxR production is controlled by the tet repressible R0040. Initial characterization work has been done on this device using I13273 as a test device.
mutable notes: Example Data Sheets for F2620
mutable provenance: Formerly I13270
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Signalling
role: SO:0000804
component: BBa_C0062
component: BBa_B0034
component: BBa_R0062
component: BBa_R0040
component: BBa_B0015
sequence annotation: BBa_B0015
sequence annotation: BBa_R0040
sequence annotation: BBa_R0062
sequence annotation: BBa_B0034
sequence annotation: BBa_C0062
sequence: https://synbiohub.org/public/igem/BBa_F2620_sequence/1

BBa_C0062
type: Component
persistent identity: component2259898
display id: component2259898
version: 1
top level: BBa_F2620
access: public
definition: https://synbiohub.org/public/igem/BBa_C0062/1

BBa_B0034
type: Component
persistent identity: component2259895
display id: component2259895
version: 1
top level: BBa_F2620
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_R0062
type: Component
persistent identity: component2259910
display id: component2259910
version: 1
top level: BBa_F2620
access: public
definition: https://synbiohub.org/public/igem/BBa_R0062/1

BBa_R0040
type: Component
persistent identity: component2259889
display id: component2259889
version: 1
top level: BBa_F2620
access: public
definition: https://synbiohub.org/public/igem/BBa_R0040/1

BBa_B0015
type: Component
persistent identity: component2259908
display id: component2259908
version: 1
top level: BBa_F2620
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2259908
display id: annotation2259908
version: 1
top level: BBa_F2620
location: https://synbiohub.org/public/igem/BBa_F2620/annotation2259908/range2259908/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_F2620/annotation2259908/range2259908/1
type: Range
persistent identity: range2259908
display id: range2259908
version: 1
direction: direction/reverse
top level: BBa_F2620
start: 870
end: 998
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation2259889
display id: annotation2259889
version: 1
top level: BBa_F2620
location: https://synbiohub.org/public/igem/BBa_F2620/annotation2259889/range2259889/1
component: BBa_R0040

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type: Range
persistent identity: range2259889
display id: range2259889
version: 1
direction: direction/forward
top level: BBa_F2620
start: 1
end: 54
orientation: inline

BBa_R0062
type: SequenceAnnotation
persistent identity: annotation2259910
display id: annotation2259910
version: 1
top level: BBa_F2620
location: https://synbiohub.org/public/igem/BBa_F2620/annotation2259910/range2259910/1
component: BBa_R0062

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type: Range
persistent identity: range2259910
display id: range2259910
version: 1
direction: direction/forward
top level: BBa_F2620
start: 1007
end: 1061
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2259895
display id: annotation2259895
version: 1
top level: BBa_F2620
location: https://synbiohub.org/public/igem/BBa_F2620/annotation2259895/range2259895/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_F2620/annotation2259895/range2259895/1
type: Range
persistent identity: range2259895
display id: range2259895
version: 1
direction: direction/reverse
top level: BBa_F2620
start: 63
end: 74
orientation: inline

BBa_C0062
type: SequenceAnnotation
persistent identity: annotation2259898
display id: annotation2259898
version: 1
top level: BBa_F2620
location: https://synbiohub.org/public/igem/BBa_F2620/annotation2259898/range2259898/1
component: BBa_C0062

https://synbiohub.org/public/igem/BBa_F2620/annotation2259898/range2259898/1
type: Range
persistent identity: range2259898
display id: range2259898
version: 1
direction: direction/forward
top level: BBa_F2620
start: 81
end: 836
orientation: inline

BBa_F2710
type: ComponentDefinition
persistent identity: BBa F2710
display id: BBa_F2710
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: N-butyryl-HSL Receiver Device
creator: labnoa
created: 2005-06-23T11:00:00Z
modified: 2015-08-31T04:07:27Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_
m user id: 0
owner id: 371
owning group id: 11
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This device senses the N-butyryl-HSL concentration in the media and produces output PoPS. CinR production is controlled by the tet repressible R0040.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Signalling
role: SO:0000804
component: BBa_R0071
component: BBa_C0171
component: BBa_B0012
component: BBa_B0010
component: BBa_R0040
component: BBa_B0034
sequence annotation: BBa_C0171
sequence annotation: BBa_R0071
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_R0040
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_F2710_sequence/1

BBa_R0071
type: Component
persistent identity: component1538922
display id: component1538922
version: 1
top level: BBa_F2710
access: public
definition: https://synbiohub.org/public/igem/BBa_R0071/1

BBa_C0171
type: Component
persistent identity: component1538891
display id: component1538891
version: 1
top level: BBa_F2710
access: public
definition: https://synbiohub.org/public/igem/BBa_C0171/1

BBa_B0012
type: Component
persistent identity: component1538906
display id: component1538906
version: 1
top level: BBa_F2710
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0010
type: Component
persistent identity: component1538896
display id: component1538896
version: 1
top level: BBa_F2710
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_R0040
type: Component
persistent identity: component1538881
display id: component1538881
version: 1
top level: BBa_F2710
access: public
definition: https://synbiohub.org/public/igem/BBa_R0040/1

BBa_B0034
type: Component
persistent identity: component1538889
display id: component1538889
version: 1
top level: BBa_F2710
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_C0171
type: SequenceAnnotation
persistent identity: annotation1538891
display id: annotation1538891
version: 1
top level: BBa_F2710
location: https://synbiohub.org/public/igem/BBa_F2710/annotation1538891/range1538891/1
component: BBa_C0171

https://synbiohub.org/public/igem/BBa_F2710/annotation1538891/range1538891/1
type: Range
persistent identity: range1538891
display id: range1538891
version: 1
direction: direction/forward
top level: BBa_F2710
start: 81
end: 809
orientation: inline

BBa_R0071
type: SequenceAnnotation
persistent identity: annotation1538922
display id: annotation1538922
version: 1
top level: BBa_F2710
location: https://synbiohub.org/public/igem/BBa_F2710/annotation1538922/range1538922/1
component: BBa_R0071

https://synbiohub.org/public/igem/BBa_F2710/annotation1538922/range1538922/1
type: Range
persistent identity: range1538922
display id: range1538922
version: 1
direction: direction/forward
top level: BBa_F2710
start: 955
end: 1007
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1538896
display id: annotation1538896
version: 1
top level: BBa_F2710
location: https://synbiohub.org/public/igem/BBa_F2710/annotation1538896/range1538896/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_F2710/annotation1538896/range1538896/1
type: Range
persistent identity: range1538896
display id: range1538896
version: 1
direction: direction/forward
top level: BBa_F2710
start: 818
end: 897
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1538906
display id: annotation1538906
version: 1
top level: BBa_F2710
location: https://synbiohub.org/public/igem/BBa_F2710/annotation1538906/range1538906/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_F2710/annotation1538906/range1538906/1
type: Range
persistent identity: range1538906
display id: range1538906
version: 1
direction: direction/forward
top level: BBa_F2710
start: 906
end: 946
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1538881
display id: annotation1538881
version: 1
top level: BBa_F2710
location: https://synbiohub.org/public/igem/BBa_F2710/annotation1538881/range1538881/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_F2710/annotation1538881/range1538881/1
type: Range
persistent identity: range1538881
display id: range1538881
version: 1
direction: direction/forward
top level: BBa_F2710
start: 1
end: 54
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1538889
display id: annotation1538889
version: 1
top level: BBa_F2710
location: https://synbiohub.org/public/igem/BBa_F2710/annotation1538889/range1538889/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_F2710/annotation1538889/range1538889/1
type: Range
persistent identity: range1538889
display id: range1538889
version: 1
direction: direction/forward
top level: BBa_F2710
start: 63
end: 74
orientation: inline

BBa_K546001
type: ComponentDefinition
persistent identity: BBa K546001
display id: BBa_K546001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Lux pL controlled luxR with lux pR controlled AHL degrading enzyme AiiA(lva tag).
creator: M. Hesselman
created: 2011-09-13T11:00:00Z
modified: 2015-05-08T01:12:39Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _713_
m user id: 0
owner id: 9055
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: The F2621 BioBrick device contains a lux pL regulated luxR repressor/activator and a lux pR promoter that is linked to an 3OC6HSL, an AHL autoinducer molecule, synthetase. The lux pL promoter yields a weak constitutive downstream expression. When LuxR and 3OC6HSL are produced, the lux pL promoter, however, will be repressed by a complex of the two. If 3OC6HSL is present in the cell, the autoinducer inactivation enzyme A (''aiiA''), with an LVA Tag, will inactivate the 3OC6HSL autoinducer molecules.
mutable notes: The original R0063 BioBrick part (promoter) of the F2621 BioBrick device turned out to be absent in our construct, as was shown by a sequence analysis. To increase the transcription activation there has been put an extra R0063 BioBrick part promoter in front of the F2621 BioBrick device.
mutable provenance: The lux p promoters originate from the quorum sensing ability of ''Vibrio fischeri''.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_I0460
component: BBa_F2621
sequence annotation: BBa_F2621
sequence annotation: BBa_I0460
sequence: https://synbiohub.org/public/igem/BBa_K546001_sequence/1

BBa_I0460
type: Component
persistent identity: component2128248
display id: component2128248
version: 1
top level: BBa_K546001
access: public
definition: https://synbiohub.org/public/igem/BBa_I0460/1

BBa_F2621
type: Component
persistent identity: component2128233
display id: component2128233
version: 1
top level: BBa_K546001
access: public
definition: BBa_F2621

BBa_F2621
type: SequenceAnnotation
persistent identity: annotation2128233
display id: annotation2128233
version: 1
top level: BBa_K546001
location: https://synbiohub.org/public/igem/BBa_K546001/annotation2128233/range2128233/1
component: BBa_F2621

https://synbiohub.org/public/igem/BBa_K546001/annotation2128233/range2128233/1
type: Range
persistent identity: range2128233
display id: range2128233
version: 1
direction: direction/reverse
top level: BBa_K546001
start: 1
end: 1158
orientation: inline

BBa_I0460
type: SequenceAnnotation
persistent identity: annotation2128248
display id: annotation2128248
version: 1
top level: BBa_K546001
location: https://synbiohub.org/public/igem/BBa_K546001/annotation2128248/range2128248/1
component: BBa_I0460

https://synbiohub.org/public/igem/BBa_K546001/annotation2128248/range2128248/1
type: Range
persistent identity: range2128248
display id: range2128248
version: 1
direction: direction/reverse
top level: BBa_K546001
start: 1167
end: 2135
orientation: inline

BBa_K772004
type: ComponentDefinition
persistent identity: BBa K772004
display id: BBa_K772004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: AHL regulated PoPS generator: Las receiver device
creator: Mustafa Elitok
created: 2012-09-24T11:00:00Z
modified: 2015-05-08T01:13:15Z
discontinued: false
dominant: false
group u list: _1024_
m user id: 0
owner id: 9573
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Quorum sensing is the mechanism of communication between bacteria via synthesizing and detecting chemical compounds in the media. With these mediators, the promoters and their yield can be tuned or regulated. This part represents an alternative quorum sensing receiver device, which is able to generate a PoPS response in the presence of 6HSLO3 produced by LasI, instead of BBa_F2620. This will allow iGEMers to establish communications between different bacteria colonies and it makes simple to generate easy regulated complex systems. This part is assembled with Las promoter system to improve the current quorum sensing devices and parts which commonly base on Lux promoter system.
mutable notes: Note that pLas has no RBS afterwards, the assembling methods must be selected after this consideration.
mutable provenance: pLas and LasR sequences are gathered from Registry.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: LasR
sequence annotation: RBS
sequence annotation: pLas
sequence annotation: pTetR
sequence annotation: Double Terminator
sequence: https://synbiohub.org/public/igem/BBa_K772004_sequence/1

LasR
type: SequenceAnnotation
persistent identity: annotation2203689
display id: annotation2203689
version: 1
top level: BBa_K772004
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K772004/annotation2203689/range2203689/1

https://synbiohub.org/public/igem/BBa_K772004/annotation2203689/range2203689/1
type: Range
persistent identity: range2203689
display id: range2203689
version: 1
direction: direction/forward
top level: BBa_K772004
start: 76
end: 824
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2203688
display id: annotation2203688
version: 1
top level: BBa_K772004
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K772004/annotation2203688/range2203688/1

https://synbiohub.org/public/igem/BBa_K772004/annotation2203688/range2203688/1
type: Range
persistent identity: range2203688
display id: range2203688
version: 1
direction: direction/forward
top level: BBa_K772004
start: 55
end: 75
orientation: inline

pLas
type: SequenceAnnotation
persistent identity: annotation2203691
display id: annotation2203691
version: 1
top level: BBa_K772004
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K772004/annotation2203691/range2203691/1

https://synbiohub.org/public/igem/BBa_K772004/annotation2203691/range2203691/1
type: Range
persistent identity: range2203691
display id: range2203691
version: 1
direction: direction/forward
top level: BBa_K772004
start: 955
end: 1122
orientation: inline

pTetR
type: SequenceAnnotation
persistent identity: annotation2203687
display id: annotation2203687
version: 1
top level: BBa_K772004
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K772004/annotation2203687/range2203687/1

https://synbiohub.org/public/igem/BBa_K772004/annotation2203687/range2203687/1
type: Range
persistent identity: range2203687
display id: range2203687
version: 1
direction: direction/forward
top level: BBa_K772004
start: 1
end: 54
orientation: inline

Double Terminator
type: SequenceAnnotation
persistent identity: annotation2203690
display id: annotation2203690
version: 1
top level: BBa_K772004
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K772004/annotation2203690/range2203690/1

https://synbiohub.org/public/igem/BBa_K772004/annotation2203690/range2203690/1
type: Range
persistent identity: range2203690
display id: range2203690
version: 1
direction: direction/forward
top level: BBa_K772004
start: 825
end: 954
orientation: inline

BBa_F2770
type: ComponentDefinition
persistent identity: BBa F2770
display id: BBa_F2770
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: 3OC14HSL Receiver Device
creator: labnoa
created: 2005-06-23T11:00:00Z
modified: 2015-08-31T04:07:27Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_
m user id: 0
owner id: 371
owning group id: 11
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This device senses the 3OC14HSL concentration in the media and produces output PoPS. CinR production is controlled by the tet repressible R0040.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Signalling
role: SO:0000804
component: BBa_B0034
component: BBa_B0010
component: BBa_C0177
component: BBa_R0077
component: BBa_R0040
component: BBa_B0012
sequence annotation: BBa_C0177
sequence annotation: BBa_B0012
sequence annotation: BBa_R0040
sequence annotation: BBa_B0034
sequence annotation: BBa_B0010
sequence annotation: BBa_R0077
sequence: https://synbiohub.org/public/igem/BBa_F2770_sequence/1

BBa_B0034
type: Component
persistent identity: component1537963
display id: component1537963
version: 1
top level: BBa_F2770
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0010
type: Component
persistent identity: component1537970
display id: component1537970
version: 1
top level: BBa_F2770
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_C0177
type: Component
persistent identity: component1537965
display id: component1537965
version: 1
top level: BBa_F2770
access: public
definition: https://synbiohub.org/public/igem/BBa_C0177/1

BBa_R0077
type: Component
persistent identity: component1537997
display id: component1537997
version: 1
top level: BBa_F2770
access: public
definition: https://synbiohub.org/public/igem/BBa_R0077/1

BBa_R0040
type: Component
persistent identity: component1537955
display id: component1537955
version: 1
top level: BBa_F2770
access: public
definition: https://synbiohub.org/public/igem/BBa_R0040/1

BBa_B0012
type: Component
persistent identity: component1537980
display id: component1537980
version: 1
top level: BBa_F2770
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_C0177
type: SequenceAnnotation
persistent identity: annotation1537965
display id: annotation1537965
version: 1
top level: BBa_F2770
location: https://synbiohub.org/public/igem/BBa_F2770/annotation1537965/range1537965/1
component: BBa_C0177

https://synbiohub.org/public/igem/BBa_F2770/annotation1537965/range1537965/1
type: Range
persistent identity: range1537965
display id: range1537965
version: 1
direction: direction/forward
top level: BBa_F2770
start: 81
end: 809
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1537980
display id: annotation1537980
version: 1
top level: BBa_F2770
location: https://synbiohub.org/public/igem/BBa_F2770/annotation1537980/range1537980/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_F2770/annotation1537980/range1537980/1
type: Range
persistent identity: range1537980
display id: range1537980
version: 1
direction: direction/forward
top level: BBa_F2770
start: 906
end: 946
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1537955
display id: annotation1537955
version: 1
top level: BBa_F2770
location: https://synbiohub.org/public/igem/BBa_F2770/annotation1537955/range1537955/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_F2770/annotation1537955/range1537955/1
type: Range
persistent identity: range1537955
display id: range1537955
version: 1
direction: direction/forward
top level: BBa_F2770
start: 1
end: 54
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1537963
display id: annotation1537963
version: 1
top level: BBa_F2770
location: https://synbiohub.org/public/igem/BBa_F2770/annotation1537963/range1537963/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_F2770/annotation1537963/range1537963/1
type: Range
persistent identity: range1537963
display id: range1537963
version: 1
direction: direction/forward
top level: BBa_F2770
start: 63
end: 74
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1537970
display id: annotation1537970
version: 1
top level: BBa_F2770
location: https://synbiohub.org/public/igem/BBa_F2770/annotation1537970/range1537970/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_F2770/annotation1537970/range1537970/1
type: Range
persistent identity: range1537970
display id: range1537970
version: 1
direction: direction/forward
top level: BBa_F2770
start: 818
end: 897
orientation: inline

BBa_R0077
type: SequenceAnnotation
persistent identity: annotation1537997
display id: annotation1537997
version: 1
top level: BBa_F2770
location: https://synbiohub.org/public/igem/BBa_F2770/annotation1537997/range1537997/1
component: BBa_R0077

https://synbiohub.org/public/igem/BBa_F2770/annotation1537997/range1537997/1
type: Range
persistent identity: range1537997
display id: range1537997
version: 1
direction: direction/forward
top level: BBa_F2770
start: 955
end: 1185
orientation: inline

BBa_K621001
type: ComponentDefinition
persistent identity: BBa K621001
display id: BBa_K621001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: OMP-R-->RBS-->eYFP
creator: David Pohlman, Blythe Ferriere, Aile Able, Erica McFarland
created: 2011-09-26T11:00:00Z
modified: 2015-05-08T01:12:55Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _793_
m user id: 0
owner id: 4838
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: Our part consists of an OMP-R binding region that regulates our reporter gene, eYFP. The OMP-R binding region is an omp-c operator, which has three OMP-R binding sites. Our part is regulated by the ENVZ-OMPR two-component regulatory system. ENVZ is a osmolarity sensor on the inner membrane and phosphorylates OMP-R in the presence of high osmolarity. When phosphorylated, OMP-R binds to the omp-c operator and recruits RNAP to transcribe the downstream reporter gene. Fluorescence intensity due to the production of eYFP is a measure of the system's activity. When all three binding sites are occupied by phosphorylated OMP-R proteins, RNAP cannot bind to the DNA and transcribe downstream. This part can be used as an osmolarity indicator. Our team used this part as a glucose sensor because the presence of glucose changes osmolarity. Different concentrations of glucose produce different, quantifiable fluorescence intensities.
mutable notes: Our team had to combine the genes in a specific order. First our team cut out and purified the eYPF gene. We added this gene to a plasmid containing RBS. Next we cut out the RBS+eYFP intermediate and ligated them into the plasmid containing OMP-R. The RBS and OMP-R parts were too small to run out and extract on a gel because they would run off of the end of the gel. Therefore, we had to start the process with eYPF.
mutable provenance: This BioBrick was created from parts found in the Registry. These parts were BBa_R0082, BBa_B0032, and BBa_E0030.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_E0030
component: BBa_R0082
component: BBa_B0032
sequence annotation: BBa_B0032
sequence annotation: BBa_R0082
sequence annotation: BBa_E0030
sequence: https://synbiohub.org/public/igem/BBa_K621001_sequence/1

BBa_E0030
type: Component
persistent identity: component2145049
display id: component2145049
version: 1
top level: BBa_K621001
access: public
definition: https://synbiohub.org/public/igem/BBa_E0030/1

BBa_R0082
type: Component
persistent identity: component2145045
display id: component2145045
version: 1
top level: BBa_K621001
access: public
definition: https://synbiohub.org/public/igem/BBa_R0082/1

BBa_B0032
type: Component
persistent identity: component2145047
display id: component2145047
version: 1
top level: BBa_K621001
access: public
definition: https://synbiohub.org/public/igem/BBa_B0032/1

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation2145047
display id: annotation2145047
version: 1
top level: BBa_K621001
location: https://synbiohub.org/public/igem/BBa_K621001/annotation2145047/range2145047/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_K621001/annotation2145047/range2145047/1
type: Range
persistent identity: range2145047
display id: range2145047
version: 1
direction: direction/forward
top level: BBa_K621001
start: 117
end: 129
orientation: inline

BBa_R0082
type: SequenceAnnotation
persistent identity: annotation2145045
display id: annotation2145045
version: 1
top level: BBa_K621001
location: https://synbiohub.org/public/igem/BBa_K621001/annotation2145045/range2145045/1
component: BBa_R0082

https://synbiohub.org/public/igem/BBa_K621001/annotation2145045/range2145045/1
type: Range
persistent identity: range2145045
display id: range2145045
version: 1
direction: direction/reverse
top level: BBa_K621001
start: 1
end: 108
orientation: inline

BBa_E0030
type: SequenceAnnotation
persistent identity: annotation2145049
display id: annotation2145049
version: 1
top level: BBa_K621001
location: https://synbiohub.org/public/igem/BBa_K621001/annotation2145049/range2145049/1
component: BBa_E0030

https://synbiohub.org/public/igem/BBa_K621001/annotation2145049/range2145049/1
type: Range
persistent identity: range2145049
display id: range2145049
version: 1
direction: direction/reverse
top level: BBa_K621001
start: 136
end: 858
orientation: inline

BBa_K574001
type: ComponentDefinition
persistent identity: BBa K574001
display id: BBa_K574001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TetR regulated by 3OC6HSL
creator: Lei Wei
created: 2011-09-28T11:00:00Z
modified: 2015-05-08T01:12:44Z
discontinued: false
dominant: false
experience: experience/None
group u list: _745_
m user id: 0
owner id: 8529
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: luxI Generator + p(luxR) +tetR.
mutable notes: Empty.
mutable provenance: Existing Parts form PartRegistry.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Signalling
role: SO:0000804
component: BBa_K145270
component: BBa_K574000
sequence annotation: BBa_K145270
sequence annotation: BBa_K574000
sequence: https://synbiohub.org/public/igem/BBa_K574001_sequence/1

BBa_K145270
type: Component
persistent identity: component2289927
display id: component2289927
version: 1
top level: BBa_K574001
access: public
definition: https://synbiohub.org/public/igem/BBa_K145270/1

BBa_K574000
type: Component
persistent identity: component2289946
display id: component2289946
version: 1
top level: BBa_K574001
access: public
definition: https://synbiohub.org/public/igem/BBa_K574000/1

BBa_K145270
type: SequenceAnnotation
persistent identity: annotation2289927
display id: annotation2289927
version: 1
top level: BBa_K574001
location: https://synbiohub.org/public/igem/BBa_K574001/annotation2289927/range2289927/1
component: BBa_K145270

https://synbiohub.org/public/igem/BBa_K574001/annotation2289927/range2289927/1
type: Range
persistent identity: range2289927
display id: range2289927
version: 1
direction: direction/reverse
top level: BBa_K574001
start: 1
end: 979
orientation: inline

BBa_K574000
type: SequenceAnnotation
persistent identity: annotation2289946
display id: annotation2289946
version: 1
top level: BBa_K574001
location: https://synbiohub.org/public/igem/BBa_K574001/annotation2289946/range2289946/1
component: BBa_K574000

https://synbiohub.org/public/igem/BBa_K574001/annotation2289946/range2289946/1
type: Range
persistent identity: range2289946
display id: range2289946
version: 1
direction: direction/reverse
top level: BBa_K574001
start: 988
end: 1890
orientation: inline

BBa_K990001
type: ComponentDefinition
persistent identity: BBa K990001
display id: BBa_K990001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pOmpC+EPIC Firefly Luciferase
creator: Fatma Bet??l ??EVİK
created: 2013-06-20T11:00:00Z
modified: 2015-05-08T01:13:50Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1287_
m user id: 0
owner id: 11713
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This part is the one of the responding systems of the receiver device of our Photocommunication system
mutable notes: We used ncbi when we couldn't find sequence
mutable provenance: EPIC Luciferase: BBa_K325100, Cambridge 2010 pOmpC: BBa_R0082
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Signalling
role: SO:0000804
component: BBa_R0082
component: BBa_K325100
sequence annotation: BBa_K325100
sequence annotation: BBa_R0082
sequence: https://synbiohub.org/public/igem/BBa_K990001_sequence/1

BBa_R0082
type: Component
persistent identity: component2329850
display id: component2329850
version: 1
top level: BBa_K990001
access: public
definition: https://synbiohub.org/public/igem/BBa_R0082/1

BBa_K325100
type: Component
persistent identity: component2329858
display id: component2329858
version: 1
top level: BBa_K990001
access: public
definition: https://synbiohub.org/public/igem/BBa_K325100/1

BBa_K325100
type: SequenceAnnotation
persistent identity: annotation2329858
display id: annotation2329858
version: 1
top level: BBa_K990001
location: https://synbiohub.org/public/igem/BBa_K990001/annotation2329858/range2329858/1
component: BBa_K325100

https://synbiohub.org/public/igem/BBa_K990001/annotation2329858/range2329858/1
type: Range
persistent identity: range2329858
display id: range2329858
version: 1
direction: direction/reverse
top level: BBa_K990001
start: 117
end: 2742
orientation: inline

BBa_R0082
type: SequenceAnnotation
persistent identity: annotation2329850
display id: annotation2329850
version: 1
top level: BBa_K990001
location: https://synbiohub.org/public/igem/BBa_K990001/annotation2329850/range2329850/1
component: BBa_R0082

https://synbiohub.org/public/igem/BBa_K990001/annotation2329850/range2329850/1
type: Range
persistent identity: range2329850
display id: range2329850
version: 1
direction: direction/reverse
top level: BBa_K990001
start: 1
end: 108
orientation: inline

BBa_K546002
type: ComponentDefinition
persistent identity: BBa K546002
display id: BBa_K546002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Lux pL controlled luxR with lux pR controlled GFP(lva tag).
creator: M. Hesselman
created: 2011-09-13T11:00:00Z
modified: 2015-05-08T01:12:39Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _713_
m user id: 0
owner id: 9055
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This BioBrick system is able to notify the downstream activation of the lux R promoter of the 3OC6HSL Receiver Device.
mutable notes: The original R0063 BioBrick part (promoter) of the F2621 BioBrick device turned out to be absent in our construct, as was shown by a sequence analysis. To increase the transcription activation there has been put an extra R0063 BioBrick part promoter in front of the F2621 BioBrick device.
mutable provenance: The lux p promoters originate from the quorum sensing ability of ''Vibrio fischeri''.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Measurement
component: BBa_F2621
component: BBa_I763020
sequence annotation: BBa_I763020
sequence annotation: BBa_F2621
sequence: https://synbiohub.org/public/igem/BBa_K546002_sequence/1

BBa_F2621
type: Component
persistent identity: component2128274
display id: component2128274
version: 1
top level: BBa_K546002
access: public
definition: BBa_F2621

BBa_I763020
type: Component
persistent identity: component2128288
display id: component2128288
version: 1
top level: BBa_K546002
access: public
definition: https://synbiohub.org/public/igem/BBa_I763020/1

BBa_I763020
type: SequenceAnnotation
persistent identity: annotation2128288
display id: annotation2128288
version: 1
top level: BBa_K546002
location: https://synbiohub.org/public/igem/BBa_K546002/annotation2128288/range2128288/1
component: BBa_I763020

https://synbiohub.org/public/igem/BBa_K546002/annotation2128288/range2128288/1
type: Range
persistent identity: range2128288
display id: range2128288
version: 1
direction: direction/reverse
top level: BBa_K546002
start: 1167
end: 2080
orientation: inline

BBa_F2621
type: SequenceAnnotation
persistent identity: annotation2128274
display id: annotation2128274
version: 1
top level: BBa_K546002
location: https://synbiohub.org/public/igem/BBa_K546002/annotation2128274/range2128274/1
component: BBa_F2621

https://synbiohub.org/public/igem/BBa_K546002/annotation2128274/range2128274/1
type: Range
persistent identity: range2128274
display id: range2128274
version: 1
direction: direction/reverse
top level: BBa_K546002
start: 1
end: 1158
orientation: inline

BBa_F2790
type: ComponentDefinition
persistent identity: BBa F2790
display id: BBa_F2790
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: AI-1 Receiver Device
creator: labnoa
created: 2005-06-23T11:00:00Z
modified: 2015-08-31T04:07:27Z
discontinued: false
dominant: false
experience: experience/None
group u list: _9_
m user id: 0
owner id: 371
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This device senses the AI-1 concentration in the media and produces output PoPS. LasR production is controlled by the tet repressible R0040.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Signalling
role: SO:0000804
component: BBa_B0012
component: BBa_R0040
component: BBa_R0079
component: BBa_B0010
component: BBa_B0034
component: BBa_C0179
sequence annotation: BBa_R0040
sequence annotation: BBa_R0079
sequence annotation: BBa_B0034
sequence annotation: BBa_C0179
sequence annotation: BBa_B0012
sequence annotation: BBa_B0010
sequence: https://synbiohub.org/public/igem/BBa_F2790_sequence/1

BBa_B0012
type: Component
persistent identity: component1538753
display id: component1538753
version: 1
top level: BBa_F2790
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_R0040
type: Component
persistent identity: component1538728
display id: component1538728
version: 1
top level: BBa_F2790
access: public
definition: https://synbiohub.org/public/igem/BBa_R0040/1

BBa_R0079
type: Component
persistent identity: component1538770
display id: component1538770
version: 1
top level: BBa_F2790
access: public
definition: https://synbiohub.org/public/igem/BBa_R0079/1

BBa_B0010
type: Component
persistent identity: component1538743
display id: component1538743
version: 1
top level: BBa_F2790
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0034
type: Component
persistent identity: component1538736
display id: component1538736
version: 1
top level: BBa_F2790
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_C0179
type: Component
persistent identity: component1538738
display id: component1538738
version: 1
top level: BBa_F2790
access: public
definition: https://synbiohub.org/public/igem/BBa_C0179/1

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1538728
display id: annotation1538728
version: 1
top level: BBa_F2790
location: https://synbiohub.org/public/igem/BBa_F2790/annotation1538728/range1538728/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_F2790/annotation1538728/range1538728/1
type: Range
persistent identity: range1538728
display id: range1538728
version: 1
direction: direction/forward
top level: BBa_F2790
start: 1
end: 54
orientation: inline

BBa_R0079
type: SequenceAnnotation
persistent identity: annotation1538770
display id: annotation1538770
version: 1
top level: BBa_F2790
location: https://synbiohub.org/public/igem/BBa_F2790/annotation1538770/range1538770/1
component: BBa_R0079

https://synbiohub.org/public/igem/BBa_F2790/annotation1538770/range1538770/1
type: Range
persistent identity: range1538770
display id: range1538770
version: 1
direction: direction/forward
top level: BBa_F2790
start: 949
end: 1105
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1538736
display id: annotation1538736
version: 1
top level: BBa_F2790
location: https://synbiohub.org/public/igem/BBa_F2790/annotation1538736/range1538736/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_F2790/annotation1538736/range1538736/1
type: Range
persistent identity: range1538736
display id: range1538736
version: 1
direction: direction/forward
top level: BBa_F2790
start: 63
end: 74
orientation: inline

BBa_C0179
type: SequenceAnnotation
persistent identity: annotation1538738
display id: annotation1538738
version: 1
top level: BBa_F2790
location: https://synbiohub.org/public/igem/BBa_F2790/annotation1538738/range1538738/1
component: BBa_C0179

https://synbiohub.org/public/igem/BBa_F2790/annotation1538738/range1538738/1
type: Range
persistent identity: range1538738
display id: range1538738
version: 1
direction: direction/forward
top level: BBa_F2790
start: 81
end: 803
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1538753
display id: annotation1538753
version: 1
top level: BBa_F2790
location: https://synbiohub.org/public/igem/BBa_F2790/annotation1538753/range1538753/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_F2790/annotation1538753/range1538753/1
type: Range
persistent identity: range1538753
display id: range1538753
version: 1
direction: direction/forward
top level: BBa_F2790
start: 900
end: 940
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1538743
display id: annotation1538743
version: 1
top level: BBa_F2790
location: https://synbiohub.org/public/igem/BBa_F2790/annotation1538743/range1538743/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_F2790/annotation1538743/range1538743/1
type: Range
persistent identity: range1538743
display id: range1538743
version: 1
direction: direction/forward
top level: BBa_F2790
start: 812
end: 891
orientation: inline