//collections/antimicrobial
type: Collection
persistent identity: collections antimicrobial
display id: collections_antimicrobial
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
owned by: james
owned by: myers
member: BBa_K1022102
member: LL-37
member: BBa_K1162001
member: BBa_K258002
member: Yeb-Art175
member: BBa_K1769009
member: BBa_K1727003
member: BBa_K1162006
member: BBa_K1647018
member: BBa_K802006
member: BBa_K1351043
member: RFC 37
member: BBa_K1727006
member: Fla-Art175
member: D4E1
member: SboA
member: BBa_K1727000
member: BBa_K1714005
member: BBa_K1351012
member: BBa_K1162003
member: BBa_K1595031
member: BBa_K1162004
member: BBa_K1364011
member: BBa_K1351013
member: BBa_K1396002
member: BBa_K1810003
member: BBa_K1810002
member: BBa_K1162005
member: BBa_K1727008
member: BBa_K1639007
member: ArtE
member: BBa_K1364019
member: BBa_K1742007
member: BBa_K1714007
member: BBa_K1022103
member: BBa_K1727007
member: BBa_K1162002
member: BBa_K1162007
member: MccS
member: BBa_K1022112
member: BBa_K875009
member: Dsb-Art175
member: BBa_K1835501
member: BBa_K1647017
member: BBa_K628006
member: BBa_K802009
member: BBa_K1714000
member: BBa_K1230011
member: BBa_K1647020
member: Art175
member: BBa_K1394000
member: BBa_K1396000
member: BBa_K1727005
member: BBa_K1162008
member: BBa_K660200
member: BBa_K1394001
member: BBa_K628000
member: BBa_K1937007

BBa_K1714005
type: ComponentDefinition
persistent identity: BBa K1714005
display id: BBa_K1714005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pBad/araC-RBS B0034-Ag43 thanatin 2mer as alpha domain-double terminator
creator: Hiroki ONO
created: 2015-09-12T11:00:00Z
modified: 2016-02-03T10:52:11Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2134_
m user id: 4206
owner id: 22047
owning group id: 9
status: status/Planning
bookmark: false
mutable description: coming soon
mutable notes: coming soon
mutable provenance: coming soon
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: BBa_K1714005
sequence annotation: BBa_I0500
sequence annotation: PC
sequence annotation: RBS B0034
sequence annotation: double terminator
sequence annotation: Thanatin 2mer as alpha domain
sequence annotation: AraC
sequence annotation: Ag43 beta domain
sequence annotation: Pbad
sequence annotation: BBa_B0034
sequence annotation: Ag43 Signal Peptide
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_K1714005_sequence/1

BBa_K1714005
type: SequenceAnnotation
persistent identity: annotation2453686
display id: annotation2453686
version: 1
top level: BBa_K1714005
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1714005/annotation2453686/range2453686/1

https://synbiohub.org/public/igem/BBa_K1714005/annotation2453686/range2453686/1
type: Range
persistent identity: range2453686
display id: range2453686
version: 1
direction: direction/forward
top level: BBa_K1714005
start: 1
end: 3074
orientation: inline

BBa_I0500
type: SequenceAnnotation
persistent identity: annotation2466897
display id: annotation2466897
version: 1
top level: BBa_K1714005
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1714005/annotation2466897/range2466897/1

https://synbiohub.org/public/igem/BBa_K1714005/annotation2466897/range2466897/1
type: Range
persistent identity: range2466897
display id: range2466897
version: 1
direction: direction/forward
top level: BBa_K1714005
start: 1
end: 1210
orientation: inline

PC
type: SequenceAnnotation
persistent identity: annotation2466899
display id: annotation2466899
version: 1
top level: BBa_K1714005
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1714005/annotation2466899/range2466899/1

https://synbiohub.org/public/igem/BBa_K1714005/annotation2466899/range2466899/1
type: Range
persistent identity: range2466899
display id: range2466899
version: 1
direction: direction/forward
top level: BBa_K1714005
start: 1043
end: 1114
orientation: inline

RBS B0034
type: SequenceAnnotation
persistent identity: annotation2466901
display id: annotation2466901
version: 1
top level: BBa_K1714005
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1714005/annotation2466901/range2466901/1

https://synbiohub.org/public/igem/BBa_K1714005/annotation2466901/range2466901/1
type: Range
persistent identity: range2466901
display id: range2466901
version: 1
direction: direction/forward
top level: BBa_K1714005
start: 1219
end: 1230
orientation: inline

double terminator
type: SequenceAnnotation
persistent identity: annotation2466906
display id: annotation2466906
version: 1
top level: BBa_K1714005
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1714005/annotation2466906/range2466906/1

https://synbiohub.org/public/igem/BBa_K1714005/annotation2466906/range2466906/1
type: Range
persistent identity: range2466906
display id: range2466906
version: 1
direction: direction/forward
top level: BBa_K1714005
start: 3017
end: 3146
orientation: inline

Thanatin 2mer as alpha domain
type: SequenceAnnotation
persistent identity: annotation2466903
display id: annotation2466903
version: 1
top level: BBa_K1714005
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1714005/annotation2466903/range2466903/1

https://synbiohub.org/public/igem/BBa_K1714005/annotation2466903/range2466903/1
type: Range
persistent identity: range2466903
display id: range2466903
version: 1
direction: direction/forward
top level: BBa_K1714005
start: 1402
end: 1539
orientation: inline

AraC
type: SequenceAnnotation
persistent identity: annotation2466898
display id: annotation2466898
version: 1
top level: BBa_K1714005
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1714005/annotation2466898/range2466898/1

https://synbiohub.org/public/igem/BBa_K1714005/annotation2466898/range2466898/1
type: Range
persistent identity: range2466898
display id: range2466898
version: 1
direction: direction/forward
top level: BBa_K1714005
start: 1
end: 1079
orientation: inline

Ag43 beta domain
type: SequenceAnnotation
persistent identity: annotation2466908
display id: annotation2466908
version: 1
top level: BBa_K1714005
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1714005/annotation2466908/range2466908/1

https://synbiohub.org/public/igem/BBa_K1714005/annotation2466908/range2466908/1
type: Range
persistent identity: range2466908
display id: range2466908
version: 1
direction: direction/forward
top level: BBa_K1714005
start: 1546
end: 3016
orientation: inline

Pbad
type: SequenceAnnotation
persistent identity: annotation2466900
display id: annotation2466900
version: 1
top level: BBa_K1714005
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1714005/annotation2466900/range2466900/1

https://synbiohub.org/public/igem/BBa_K1714005/annotation2466900/range2466900/1
type: Range
persistent identity: range2466900
display id: range2466900
version: 1
direction: direction/forward
top level: BBa_K1714005
start: 1080
end: 1210
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2476075
display id: annotation2476075
version: 1
top level: BBa_K1714005
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1714005/annotation2476075/range2476075/1

https://synbiohub.org/public/igem/BBa_K1714005/annotation2476075/range2476075/1
type: Range
persistent identity: range2476075
display id: range2476075
version: 1
direction: direction/forward
top level: BBa_K1714005
start: 1219
end: 1230
orientation: inline

Ag43 Signal Peptide
type: SequenceAnnotation
persistent identity: annotation2466902
display id: annotation2466902
version: 1
top level: BBa_K1714005
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1714005/annotation2466902/range2466902/1

https://synbiohub.org/public/igem/BBa_K1714005/annotation2466902/range2466902/1
type: Range
persistent identity: range2466902
display id: range2466902
version: 1
direction: direction/forward
top level: BBa_K1714005
start: 1237
end: 1395
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2476076
display id: annotation2476076
version: 1
top level: BBa_K1714005
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1714005/annotation2476076/range2476076/1

https://synbiohub.org/public/igem/BBa_K1714005/annotation2476076/range2476076/1
type: Range
persistent identity: range2476076
display id: range2476076
version: 1
direction: direction/forward
top level: BBa_K1714005
start: 3017
end: 3146
orientation: inline

BBa_K258002
type: ComponentDefinition
persistent identity: BBa K258002
display id: BBa_K258002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Granulysin, a T Cell Product,Kills Bacteria by Altering Membrane Permeability
creator: Ozkan IS
created: 2009-10-02T11:00:00Z
modified: 2016-02-01T12:59:26Z
discontinued: false
dominant: true
experience: experience/None
group u list: _358_
m user id: 4206
owner id: 4260
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: Granulysin, a protein located in the acidic granules of human NK cells and cytotoxic T cells, has microbial activity against a broad spectrum of microbial pathogens. The substance released by cytotoxic T cells (CD8) when attached to infected body cells. It creates holes in the target cell membrane and destroy it. That is,Granulysin induces apoptosis in target cells and have antimicrobial action. It is broadly antimicrobial, killing microbes that cause, for example, tuberculosis and malaria, and can destroy some tumors. A series of peptides generated from the amino acid sequence of granulysin are potential antibiotics. Granulysin increased the permeability of bacterial membranes, as judged by its ability to allow access of cytosolic ??-galactosidase to its impermeant substrate.Granulysin functions to create holes in the target cell membrane and destroy it. Granulysin is able to induce apoptosis in target cells and also has antimicrobial action.
mutable notes: Granulysin can be purified via nickel affinity chromatography.
mutable provenance: Granulysin is a member of the saposin-like protein (SAPLIP) family. The gene was synthesized at GENEART in pMA vector
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K258002_sequence/1

BBa_K875009
type: ComponentDefinition
persistent identity: BBa K875009
display id: BBa_K875009
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LL 37 - Cathelicidin
creator: Giulia Corso, Sara Samari
created: 2012-09-22T11:00:00Z
modified: 2016-01-27T02:41:40Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1136_
m user id: 4206
owner id: 12657
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: A new LL 37 bacteria toxin optimized for E. coli expression. The toxin disrupt the membrane from outside and is armless from inside.
mutable notes: by synthesis
mutable provenance: Ulrich et al., 2006
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_K875009_sequence/1

start
type: SequenceAnnotation
persistent identity: annotation2200494
display id: annotation2200494
version: 1
top level: BBa_K875009
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K875009/annotation2200494/range2200494/1

https://synbiohub.org/public/igem/BBa_K875009/annotation2200494/range2200494/1
type: Range
persistent identity: range2200494
display id: range2200494
version: 1
direction: direction/forward
top level: BBa_K875009
start: 1
end: 3
orientation: inline

Fla-Art175
type: ComponentDefinition
persistent identity: BBa K1659001
display id: BBa_K1659001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Artilysin Art-175 fused at N-terminal with flagellin 26-47 peptide segment
creator: Raphaella Hull
created: 2015-08-09T11:00:00Z
modified: 2016-02-01T12:51:52Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2077_
m user id: 4206
owner id: 24525
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Artilysins are an exciting class of enzyme-based antibacterials. Their name is derived from "artificial endolysin" and they exploit the lytic power of bacteriophage-encoded endolyins. Endolysins are peptidoglycan hydrolases produced at the end of the lytic cycle that pass through the cytoplasmic membrane, degrade the peptidoglycan layer and cause the osmotic lysis of the infected bacterial cell, thus liberating the progeny. Purified endolysins have been used to kill Gram-positive pathogens. Gram-negative bacteria, however, have a protective outer membrane containing lipopolysaccharide (LPS) that serves as a barrier against the peptidoglycan hydolytic activity of endolysins from the outside. To overcome this problem, selected polycationic or amphipathic peptides that locally destabilize the LPS layer have been covalently fused to endolysins. Biers et al. fused the sheep myeloid antimicrobial peptide (SMAP-29) to the N terminus of the endolysin KZ144 to create Artilysin 175. Art-175 has been shown to be a highly potent antibacterial that acts in minutes to kill virtually all Pseudomonas aeruginosa strains. [1] BBa_K1659001 is a composite of Art-175 with the 26-47 segment of Salmonella typhimurium flagellin combined with a hexahistidine tag. [2] Fla 26-47 is capable of mediating flagellar export of recombinant proteins and, combined with a hexahistidine tag, facilitates their secreted expression and easy purification. [1] Briers, Y., Walmagh, M., Grymonprez, B., Biebl, M., Pirnay, J. P., Defraine, V., ??? Lavigne, R. (2014). Art-175 is a highly efficient antibacterial against multidrug-resistant strains and persisters of Pseudomonas aeruginosa. Antimicrobial Agents and Chemotherapy, 58(7), 3774???3784. http://doi.org/10.1128/AAC.02668-14 [2] Balb??s, P., & Lorence, A. (2004). Recombinant Gene Expression. Reviews and Protocols, 267, 506. http://doi.org/10.1007/978-1-61779-433-9
mutable notes: Export signal combined with hexahistidine tag
mutable provenance: Art-175 amino acid sequence: Briers, Y., Walmagh, M., Grymonprez, B., Biebl, M., Pirnay, J. P., Defraine, V., ??? Lavigne, R. (2014). Art-175 is a highly efficient antibacterial against multidrug-resistant strains and persisters of Pseudomonas aeruginosa. Antimicrobial Agents and Chemotherapy, 58(7), 3774???3784. http://doi.org/10.1128/AAC.02668-14 Amino acid sequence of Fla 26-47: Balb??s, P., & Lorence, A. (2004). Recombinant Gene Expression. Reviews and Protocols, 267, 506. http://doi.org/10.1007/978-1-61779-433-9
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: stop
sequence annotation: start
sequence annotation: Flagellin 26-47 Secretion Signal Tag
sequence annotation: Art-175
sequence annotation: Hisx6
sequence: https://synbiohub.org/public/igem/BBa_K1659001_sequence/1

stop
type: SequenceAnnotation
persistent identity: annotation2438074
display id: annotation2438074
version: 1
top level: Fla-Art175
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1659001/annotation2438074/range2438074/1

https://synbiohub.org/public/igem/BBa_K1659001/annotation2438074/range2438074/1
type: Range
persistent identity: range2438074
display id: range2438074
version: 1
direction: direction/forward
top level: Fla-Art175
start: 1054
end: 1056
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2438066
display id: annotation2438066
version: 1
top level: Fla-Art175
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1659001/annotation2438066/range2438066/1

https://synbiohub.org/public/igem/BBa_K1659001/annotation2438066/range2438066/1
type: Range
persistent identity: range2438066
display id: range2438066
version: 1
direction: direction/forward
top level: Fla-Art175
start: 1
end: 3
orientation: inline

Flagellin 26-47 Secretion Signal Tag
type: SequenceAnnotation
persistent identity: annotation2438058
display id: annotation2438058
version: 1
top level: Fla-Art175
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1659001/annotation2438058/range2438058/1

https://synbiohub.org/public/igem/BBa_K1659001/annotation2438058/range2438058/1
type: Range
persistent identity: range2438058
display id: range2438058
version: 1
direction: direction/forward
top level: Fla-Art175
start: 4
end: 165
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2438072
display id: annotation2438072
version: 1
top level: Fla-Art175
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1659001/annotation2438072/range2438072/1

https://synbiohub.org/public/igem/BBa_K1659001/annotation2438072/range2438072/1
type: Range
persistent identity: range2438072
display id: range2438072
version: 1
direction: direction/forward
top level: Fla-Art175
start: 1051
end: 1053
orientation: inline

Art-175
type: SequenceAnnotation
persistent identity: annotation2438068
display id: annotation2438068
version: 1
top level: Fla-Art175
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1659001/annotation2438068/range2438068/1

https://synbiohub.org/public/igem/BBa_K1659001/annotation2438068/range2438068/1
type: Range
persistent identity: range2438068
display id: range2438068
version: 1
direction: direction/forward
top level: Fla-Art175
start: 166
end: 1032
orientation: inline

Hisx6
type: SequenceAnnotation
persistent identity: annotation2438070
display id: annotation2438070
version: 1
top level: Fla-Art175
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1659001/annotation2438070/range2438070/1

https://synbiohub.org/public/igem/BBa_K1659001/annotation2438070/range2438070/1
type: Range
persistent identity: range2438070
display id: range2438070
version: 1
direction: direction/forward
top level: Fla-Art175
start: 1033
end: 1050
orientation: inline

BBa_K1394000
type: ComponentDefinition
persistent identity: BBa K1394000
display id: BBa_K1394000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Magainin 1 star peptide
creator: Sean Lowe
created: 2014-10-04T11:00:00Z
modified: 2016-02-03T10:52:46Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1553_1771_
m user id: 4206
owner id: 17889
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This part codes for the Magainin 1 star peptide, a novel peptide design by the University of Melbourne 2014 iGEM team.
mutable notes: Because the protein coding region, Magainin 1, is likely cytotoxic, the protein is expressed using a SUMO fusion partner.
mutable provenance: The protein coding region of the peptide was designed de novo by the University of Melbourne 2014 iGEM team. It is based off of the naturally occurring Magainin 1 antimicrobial peptide. The promoter, ribosome binding site, and terminator are standard iGEM parts.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
sequence annotation: RBS
sequence annotation: Magainin 1 Star Peptide
sequence annotation: BBa_K525998
sequence annotation: HIS-tagged SUMO
sequence annotation: Cloning scar
sequence annotation: T7 terminator
sequence annotation: T7 promoter
sequence annotation: BBa_K731721
sequence: https://synbiohub.org/public/igem/BBa_K1394000_sequence/1

RBS
type: SequenceAnnotation
persistent identity: annotation2427440
display id: annotation2427440
version: 1
top level: BBa_K1394000
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1394000/annotation2427440/range2427440/1

https://synbiohub.org/public/igem/BBa_K1394000/annotation2427440/range2427440/1
type: Range
persistent identity: range2427440
display id: range2427440
version: 1
direction: direction/forward
top level: BBa_K1394000
start: 24
end: 27
orientation: inline

Magainin 1 Star Peptide
type: SequenceAnnotation
persistent identity: annotation2427949
display id: annotation2427949
version: 1
top level: BBa_K1394000
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1394000/annotation2427949/range2427949/1

https://synbiohub.org/public/igem/BBa_K1394000/annotation2427949/range2427949/1
type: Range
persistent identity: range2427949
display id: range2427949
version: 1
direction: direction/forward
top level: BBa_K1394000
start: 352
end: 651
orientation: inline

BBa_K525998
type: SequenceAnnotation
persistent identity: annotation2427439
display id: annotation2427439
version: 1
top level: BBa_K1394000
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1394000/annotation2427439/range2427439/1

https://synbiohub.org/public/igem/BBa_K1394000/annotation2427439/range2427439/1
type: Range
persistent identity: range2427439
display id: range2427439
version: 1
direction: direction/reverse
top level: BBa_K1394000
start: 1
end: 32
orientation: inline

HIS-tagged SUMO
type: SequenceAnnotation
persistent identity: annotation2427948
display id: annotation2427948
version: 1
top level: BBa_K1394000
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1394000/annotation2427948/range2427948/1

https://synbiohub.org/public/igem/BBa_K1394000/annotation2427948/range2427948/1
type: Range
persistent identity: range2427948
display id: range2427948
version: 1
direction: direction/forward
top level: BBa_K1394000
start: 40
end: 351
orientation: inline

Cloning scar
type: SequenceAnnotation
persistent identity: annotation2427947
display id: annotation2427947
version: 1
top level: BBa_K1394000
role: feature/scar
location: https://synbiohub.org/public/igem/BBa_K1394000/annotation2427947/range2427947/1

https://synbiohub.org/public/igem/BBa_K1394000/annotation2427947/range2427947/1
type: Range
persistent identity: range2427947
display id: range2427947
version: 1
direction: direction/forward
top level: BBa_K1394000
start: 33
end: 39
orientation: inline

T7 terminator
type: SequenceAnnotation
persistent identity: annotation2427441
display id: annotation2427441
version: 1
top level: BBa_K1394000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1394000/annotation2427441/range2427441/1

https://synbiohub.org/public/igem/BBa_K1394000/annotation2427441/range2427441/1
type: Range
persistent identity: range2427441
display id: range2427441
version: 1
direction: direction/forward
top level: BBa_K1394000
start: 652
end: 699
orientation: inline

T7 promoter
type: SequenceAnnotation
persistent identity: annotation2427438
display id: annotation2427438
version: 1
top level: BBa_K1394000
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1394000/annotation2427438/range2427438/1

https://synbiohub.org/public/igem/BBa_K1394000/annotation2427438/range2427438/1
type: Range
persistent identity: range2427438
display id: range2427438
version: 1
direction: direction/forward
top level: BBa_K1394000
start: 1
end: 32
orientation: inline

BBa_K731721
type: SequenceAnnotation
persistent identity: annotation2427442
display id: annotation2427442
version: 1
top level: BBa_K1394000
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1394000/annotation2427442/range2427442/1

https://synbiohub.org/public/igem/BBa_K1394000/annotation2427442/range2427442/1
type: Range
persistent identity: range2427442
display id: range2427442
version: 1
direction: direction/reverse
top level: BBa_K1394000
start: 652
end: 699
orientation: inline

BBa_K1162002
type: ComponentDefinition
persistent identity: BBa K1162002
display id: BBa_K1162002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Spheniscin-2 antimicrobial peptide from the king penguin (Aptenodytes patagonicus)
creator: Charles Barentine
created: 2013-09-14T11:00:00Z
modified: 2016-01-28T12:13:28Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1474_
m user id: 4206
owner id: 10131
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: RFC 23 compatible, contains ATG (met).
mutable notes: Codon optimized for E. Coli, contains ATG (met).
mutable provenance: King Penguin, Aptenodytes patagonicus
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Met
sequence annotation: Spheniscin-2
sequence: https://synbiohub.org/public/igem/BBa_K1162002_sequence/1

Met
type: SequenceAnnotation
persistent identity: annotation2343330
display id: annotation2343330
version: 1
top level: BBa_K1162002
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1162002/annotation2343330/range2343330/1

https://synbiohub.org/public/igem/BBa_K1162002/annotation2343330/range2343330/1
type: Range
persistent identity: range2343330
display id: range2343330
version: 1
direction: direction/forward
top level: BBa_K1162002
start: 1
end: 3
orientation: inline

Spheniscin-2
type: SequenceAnnotation
persistent identity: annotation2365913
display id: annotation2365913
version: 1
top level: BBa_K1162002
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1162002/annotation2365913/range2365913/1

https://synbiohub.org/public/igem/BBa_K1162002/annotation2365913/range2365913/1
type: Range
persistent identity: range2365913
display id: range2365913
version: 1
direction: direction/forward
top level: BBa_K1162002
start: 4
end: 117
orientation: inline

BBa_K1727000
type: ComponentDefinition
persistent identity: BBa K1727000
display id: BBa_K1727000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Signiferin with signal peptide
creator: Ying Kuan
created: 2015-09-01T11:00:00Z
modified: 2016-02-03T10:04:50Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2148_
m user id: 4206
owner id: 25489
owning group id: 9
status: status/Planning
bookmark: true
mutable description: Antimicrobial peptides (AMPs) are stable peptides that have extensive ability to kill or inhibit the growth of bacteria. They play a role in defense mechanism for all organisms, ranging from prokaryotes to humans, to against predators. Some of them act against specific strain of microbes. Signiferin is a kinds of antimicrobial peptide extracted from the skin mucus of Crinia signifera, respectively. It demonstrated effectiveness in killing MRSA. And we had change it's sequence into E.coli codon usage. We treat a signal peptide in front of signiferin. This signal peptide comes from S.lividas and has ability to let E.coli secrect signiferin to LB culture medium.
mutable notes: We had change signiferin sequence into E.coli codon usage. In order to get the antimicrobial peptide easily we treat signal peptide in front of the AMP.
mutable provenance: We synthesis this sequence from IDT (Integrated device technology) company.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Restriction enzyme site BamH I
sequence annotation: Restriction enzyme site Nco I
sequence annotation: signal peptide
sequence annotation: Signiferin
sequence: https://synbiohub.org/public/igem/BBa_K1727000_sequence/1

Restriction enzyme site BamH I
type: SequenceAnnotation
persistent identity: annotation2473447
display id: annotation2473447
version: 1
top level: BBa_K1727000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1727000/annotation2473447/range2473447/1

https://synbiohub.org/public/igem/BBa_K1727000/annotation2473447/range2473447/1
type: Range
persistent identity: range2473447
display id: range2473447
version: 1
direction: direction/forward
top level: BBa_K1727000
start: 173
end: 179
orientation: inline

Restriction enzyme site Nco I
type: SequenceAnnotation
persistent identity: annotation2473435
display id: annotation2473435
version: 1
top level: BBa_K1727000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1727000/annotation2473435/range2473435/1

https://synbiohub.org/public/igem/BBa_K1727000/annotation2473435/range2473435/1
type: Range
persistent identity: range2473435
display id: range2473435
version: 1
direction: direction/forward
top level: BBa_K1727000
start: 1
end: 6
orientation: inline

signal peptide
type: SequenceAnnotation
persistent identity: annotation2473436
display id: annotation2473436
version: 1
top level: BBa_K1727000
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1727000/annotation2473436/range2473436/1

https://synbiohub.org/public/igem/BBa_K1727000/annotation2473436/range2473436/1
type: Range
persistent identity: range2473436
display id: range2473436
version: 1
direction: direction/forward
top level: BBa_K1727000
start: 7
end: 104
orientation: inline

Signiferin
type: SequenceAnnotation
persistent identity: annotation2473437
display id: annotation2473437
version: 1
top level: BBa_K1727000
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1727000/annotation2473437/range2473437/1

https://synbiohub.org/public/igem/BBa_K1727000/annotation2473437/range2473437/1
type: Range
persistent identity: range2473437
display id: range2473437
version: 1
direction: direction/forward
top level: BBa_K1727000
start: 105
end: 173
orientation: inline

ArtE
type: ComponentDefinition
persistent identity: BBa K1659088
display id: BBa_K1659088
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Artilysin Art-E, an antibacterial fusion protein
creator: Wei Chung Kong
created: 2015-08-27T11:00:00Z
modified: 2016-02-01T12:56:44Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2077_
m user id: 4206
owner id: 24524
owning group id: 9
status: status/Planning
bookmark: false
mutable description: to be updated
mutable notes: to be updated
mutable provenance: to be updated
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: stop
sequence annotation: Hisx6
sequence annotation: SMAP-29
sequence annotation: start
sequence annotation: Art-E
sequence annotation: T4 Endolysin
sequence: https://synbiohub.org/public/igem/BBa_K1659088_sequence/1

stop
type: SequenceAnnotation
persistent identity: annotation2446601
display id: annotation2446601
version: 1
top level: ArtE
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1659088/annotation2446601/range2446601/1

https://synbiohub.org/public/igem/BBa_K1659088/annotation2446601/range2446601/1
type: Range
persistent identity: range2446601
display id: range2446601
version: 1
direction: direction/forward
top level: ArtE
start: 598
end: 603
orientation: inline

Hisx6
type: SequenceAnnotation
persistent identity: annotation2446599
display id: annotation2446599
version: 1
top level: ArtE
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1659088/annotation2446599/range2446599/1

https://synbiohub.org/public/igem/BBa_K1659088/annotation2446599/range2446599/1
type: Range
persistent identity: range2446599
display id: range2446599
version: 1
direction: direction/forward
top level: ArtE
start: 580
end: 597
orientation: inline

SMAP-29
type: SequenceAnnotation
persistent identity: annotation2446596
display id: annotation2446596
version: 1
top level: ArtE
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1659088/annotation2446596/range2446596/1

https://synbiohub.org/public/igem/BBa_K1659088/annotation2446596/range2446596/1
type: Range
persistent identity: range2446596
display id: range2446596
version: 1
direction: direction/forward
top level: ArtE
start: 4
end: 90
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2446595
display id: annotation2446595
version: 1
top level: ArtE
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1659088/annotation2446595/range2446595/1

https://synbiohub.org/public/igem/BBa_K1659088/annotation2446595/range2446595/1
type: Range
persistent identity: range2446595
display id: range2446595
version: 1
direction: direction/forward
top level: ArtE
start: 1
end: 3
orientation: inline

Art-E
type: SequenceAnnotation
persistent identity: annotation2446598
display id: annotation2446598
version: 1
top level: ArtE
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1659088/annotation2446598/range2446598/1

https://synbiohub.org/public/igem/BBa_K1659088/annotation2446598/range2446598/1
type: Range
persistent identity: range2446598
display id: range2446598
version: 1
direction: direction/forward
top level: ArtE
start: 4
end: 579
orientation: inline

T4 Endolysin
type: SequenceAnnotation
persistent identity: annotation2446597
display id: annotation2446597
version: 1
top level: ArtE
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1659088/annotation2446597/range2446597/1

https://synbiohub.org/public/igem/BBa_K1659088/annotation2446597/range2446597/1
type: Range
persistent identity: range2446597
display id: range2446597
version: 1
direction: direction/forward
top level: ArtE
start: 91
end: 579
orientation: inline

BBa_K1714007
type: ComponentDefinition
persistent identity: BBa K1714007
display id: BBa_K1714007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pBad/araC-RBS B0034-Ag43 thanatin 4mer as alpha domain-double terminator
creator: Hiroki ONO
created: 2015-09-16T11:00:00Z
modified: 2016-02-01T01:00:55Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2134_
m user id: 4206
owner id: 22047
owning group id: 9
status: status/Planning
bookmark: true
mutable description: Coming soon
mutable notes: Coming soon
mutable provenance: Coming soon
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: BBa_K1714007
sequence annotation: BBa_B0034
sequence annotation: BBa_B0015
sequence annotation: PC
sequence annotation: Thanatin 4mer as alpha domain
sequence annotation: double terminator
sequence annotation: Ag43 Signal Peptide
sequence annotation: AraC
sequence annotation: Ag43 beta domain
sequence annotation: RBS B0034
sequence annotation: Pbad
sequence annotation: BBa_I0500
sequence: https://synbiohub.org/public/igem/BBa_K1714007_sequence/1

BBa_K1714007
type: SequenceAnnotation
persistent identity: annotation2466365
display id: annotation2466365
version: 1
top level: BBa_K1714007
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1714007/annotation2466365/range2466365/1

https://synbiohub.org/public/igem/BBa_K1714007/annotation2466365/range2466365/1
type: Range
persistent identity: range2466365
display id: range2466365
version: 1
direction: direction/forward
top level: BBa_K1714007
start: 1
end: 3290
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2476070
display id: annotation2476070
version: 1
top level: BBa_K1714007
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1714007/annotation2476070/range2476070/1

https://synbiohub.org/public/igem/BBa_K1714007/annotation2476070/range2476070/1
type: Range
persistent identity: range2476070
display id: range2476070
version: 1
direction: direction/forward
top level: BBa_K1714007
start: 1219
end: 1230
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2476071
display id: annotation2476071
version: 1
top level: BBa_K1714007
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1714007/annotation2476071/range2476071/1

https://synbiohub.org/public/igem/BBa_K1714007/annotation2476071/range2476071/1
type: Range
persistent identity: range2476071
display id: range2476071
version: 1
direction: direction/forward
top level: BBa_K1714007
start: 3161
end: 3290
orientation: inline

PC
type: SequenceAnnotation
persistent identity: annotation2466985
display id: annotation2466985
version: 1
top level: BBa_K1714007
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1714007/annotation2466985/range2466985/1

https://synbiohub.org/public/igem/BBa_K1714007/annotation2466985/range2466985/1
type: Range
persistent identity: range2466985
display id: range2466985
version: 1
direction: direction/forward
top level: BBa_K1714007
start: 1043
end: 1114
orientation: inline

Thanatin 4mer as alpha domain
type: SequenceAnnotation
persistent identity: annotation2467000
display id: annotation2467000
version: 1
top level: BBa_K1714007
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1714007/annotation2467000/range2467000/1

https://synbiohub.org/public/igem/BBa_K1714007/annotation2467000/range2467000/1
type: Range
persistent identity: range2467000
display id: range2467000
version: 1
direction: direction/forward
top level: BBa_K1714007
start: 1402
end: 1683
orientation: inline

double terminator
type: SequenceAnnotation
persistent identity: annotation2467007
display id: annotation2467007
version: 1
top level: BBa_K1714007
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1714007/annotation2467007/range2467007/1

https://synbiohub.org/public/igem/BBa_K1714007/annotation2467007/range2467007/1
type: Range
persistent identity: range2467007
display id: range2467007
version: 1
direction: direction/forward
top level: BBa_K1714007
start: 3161
end: 3290
orientation: inline

Ag43 Signal Peptide
type: SequenceAnnotation
persistent identity: annotation2466989
display id: annotation2466989
version: 1
top level: BBa_K1714007
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1714007/annotation2466989/range2466989/1

https://synbiohub.org/public/igem/BBa_K1714007/annotation2466989/range2466989/1
type: Range
persistent identity: range2466989
display id: range2466989
version: 1
direction: direction/forward
top level: BBa_K1714007
start: 1237
end: 1395
orientation: inline

AraC
type: SequenceAnnotation
persistent identity: annotation2466984
display id: annotation2466984
version: 1
top level: BBa_K1714007
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1714007/annotation2466984/range2466984/1

https://synbiohub.org/public/igem/BBa_K1714007/annotation2466984/range2466984/1
type: Range
persistent identity: range2466984
display id: range2466984
version: 1
direction: direction/forward
top level: BBa_K1714007
start: 1
end: 1079
orientation: inline

Ag43 beta domain
type: SequenceAnnotation
persistent identity: annotation2467005
display id: annotation2467005
version: 1
top level: BBa_K1714007
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1714007/annotation2467005/range2467005/1

https://synbiohub.org/public/igem/BBa_K1714007/annotation2467005/range2467005/1
type: Range
persistent identity: range2467005
display id: range2467005
version: 1
direction: direction/forward
top level: BBa_K1714007
start: 1684
end: 3160
orientation: inline

RBS B0034
type: SequenceAnnotation
persistent identity: annotation2466988
display id: annotation2466988
version: 1
top level: BBa_K1714007
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1714007/annotation2466988/range2466988/1

https://synbiohub.org/public/igem/BBa_K1714007/annotation2466988/range2466988/1
type: Range
persistent identity: range2466988
display id: range2466988
version: 1
direction: direction/forward
top level: BBa_K1714007
start: 1219
end: 1230
orientation: inline

Pbad
type: SequenceAnnotation
persistent identity: annotation2466986
display id: annotation2466986
version: 1
top level: BBa_K1714007
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1714007/annotation2466986/range2466986/1

https://synbiohub.org/public/igem/BBa_K1714007/annotation2466986/range2466986/1
type: Range
persistent identity: range2466986
display id: range2466986
version: 1
direction: direction/forward
top level: BBa_K1714007
start: 1080
end: 1210
orientation: inline

BBa_I0500
type: SequenceAnnotation
persistent identity: annotation2466983
display id: annotation2466983
version: 1
top level: BBa_K1714007
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1714007/annotation2466983/range2466983/1

https://synbiohub.org/public/igem/BBa_K1714007/annotation2466983/range2466983/1
type: Range
persistent identity: range2466983
display id: range2466983
version: 1
direction: direction/forward
top level: BBa_K1714007
start: 1
end: 1210
orientation: inline

BBa_K1162008
type: ComponentDefinition
persistent identity: BBa K1162008
display id: BBa_K1162008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: OH-CATH (3-34) antimicrobial peptide from the King Cobra (Ophiophagus hannah)
creator: Ryan Putman
created: 2013-09-14T11:00:00Z
modified: 2016-01-28T12:17:33Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1474_
m user id: 4206
owner id: 13965
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: An antimicrobial peptide from the Cathelicidin family of peptides, isolated from the King Cobra (Ophiophagus hannah). Shortened version of the full 34 amino acid protein (C-terminal KR residues removed) that retains antibacterial properties while significantly removing hemolytic activity.
mutable notes: RFC 23. ATG(Met) at beginning of coding region.
mutable provenance: King Cobra (Ophiophagus hannah)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1162008_sequence/1

BBa_K1162004
type: ComponentDefinition
persistent identity: BBa K1162004
display id: BBa_K1162004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Cg-Defh1 antimicrobial peptide from the Pacific oyster (Crassostrea gigas)
creator: Charles Barentine
created: 2013-09-14T11:00:00Z
modified: 2016-01-28T12:14:01Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1474_
m user id: 4206
owner id: 10131
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: RFC 23 compatible. Contains ATG (methionine).
mutable notes: Codon optimized for E. Coli, contains ATG (met).
mutable provenance: Oyster(Crassostrea gigas)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Met
sequence annotation: CG-Defh1
sequence: https://synbiohub.org/public/igem/BBa_K1162004_sequence/1

Met
type: SequenceAnnotation
persistent identity: annotation2365854
display id: annotation2365854
version: 1
top level: BBa_K1162004
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1162004/annotation2365854/range2365854/1

https://synbiohub.org/public/igem/BBa_K1162004/annotation2365854/range2365854/1
type: Range
persistent identity: range2365854
display id: range2365854
version: 1
direction: direction/forward
top level: BBa_K1162004
start: 1
end: 3
orientation: inline

CG-Defh1
type: SequenceAnnotation
persistent identity: annotation2365874
display id: annotation2365874
version: 1
top level: BBa_K1162004
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1162004/annotation2365874/range2365874/1

https://synbiohub.org/public/igem/BBa_K1162004/annotation2365874/range2365874/1
type: Range
persistent identity: range2365874
display id: range2365874
version: 1
direction: direction/forward
top level: BBa_K1162004
start: 4
end: 132
orientation: inline

BBa_K1396002
type: ComponentDefinition
persistent identity: BBa K1396002
display id: BBa_K1396002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pSB1C3: MFP-sfGFP
creator: Ariel Hernandez-Leyva
created: 2014-10-09T11:00:00Z
modified: 2016-02-03T10:49:14Z
discontinued: false
dominant: false
group u list: _1773_
m user id: 4206
owner id: 22908
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: The part is an coding sequence for an anti-microbial peptides linked to a mussel-foot protein-linked to superfolder GFP for localization. The mussel foot protein will anneal to surfaces as a wet glue and superfolder GFP will allow for florescence imaging and localization. In order to use this part you can produce it in a TAG recoded organism simultaneously expressing a Tyrosine supressor or L-DOPA orthogonal translational system. In order to purify you can use the 2X Strep tag and strep column and later cleave with enterokinase to remove the sequence supressing LL-37 antimicrobial action.
mutable notes: There are linker units between individual components of DNA and restriction sites built for modularity.
mutable provenance: Synthesized in Lab
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: mfp-1-5-1
sequence annotation: sfGFP
sequence: https://synbiohub.org/public/igem/BBa_K1396002_sequence/1

mfp-1-5-1
type: SequenceAnnotation
persistent identity: annotation2414667
display id: annotation2414667
version: 1
top level: BBa_K1396002
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1396002/annotation2414667/range2414667/1

https://synbiohub.org/public/igem/BBa_K1396002/annotation2414667/range2414667/1
type: Range
persistent identity: range2414667
display id: range2414667
version: 1
direction: direction/forward
top level: BBa_K1396002
start: 1
end: 591
orientation: inline

sfGFP
type: SequenceAnnotation
persistent identity: annotation2414680
display id: annotation2414680
version: 1
top level: BBa_K1396002
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1396002/annotation2414680/range2414680/1

https://synbiohub.org/public/igem/BBa_K1396002/annotation2414680/range2414680/1
type: Range
persistent identity: range2414680
display id: range2414680
version: 1
direction: direction/forward
top level: BBa_K1396002
start: 634
end: 1353
orientation: inline

BBa_K1647020
type: ComponentDefinition
persistent identity: BBa K1647020
display id: BBa_K1647020
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: AAP2, a kind of antimicrobial peptides
creator: Yun Xue
created: 2015-09-14T11:00:00Z
modified: 2016-01-28T12:08:34Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2064_
m user id: 4206
owner id: 28136
owning group id: 9
status: status/Planning
bookmark: false
mutable description: The antimicrobial peptide, APP2, can achieve high activity exclusively at low environmental pH to target bacterial species like Streptococcus mutans that produce acid and thrive under the low pH conditions detrimental for tooth integrity. However, at pH 7.5, it has no effect on cell viability. There are only six histidine(His) and eight phenylalanine(Phe) residues present in the APP2. The pH-responsiveness of APP2 has been attributed to the presence of multiple His which enable the conformational flexibility necessary to transition from the less effective more general membrane disruptive α-helical form at close to neutral pH to its substantially more active random coil conformation at low pH. The hydrophobicity necessary for membrane interaction is provided by Phe groups which in contrast to other hydrophobic amino acids allow for the conformational flexibility needed for the pH-dependent antimicrobial activity of APP2.
mutable notes: None
mutable provenance: None for the moment
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1647020_sequence/1

BBa_K1714000
type: ComponentDefinition
persistent identity: BBa K1714000
display id: BBa_K1714000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pBad/araC-RBS B0034-Ag43 thanatin as alpha domain-double terminator
creator: Hiroki ONO
created: 2015-09-09T11:00:00Z
modified: 2016-02-03T10:45:45Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2134_
m user id: 4206
owner id: 22047
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Ag43 is a kind of outer membrane protein that is composed of 3 domains. First one is signal peptide that transport Ag43 to outer membrane. Second one is alpha domain that has connection peptide to another Ag43 alpha domain. Last one is beta domain that has beta barrel structure to let the barrel there. This part has thanatin, a kind of antimicrobial peptide as alpha domain. This thanatin has Asp as the last residue. So if thanatin domain is digested by Asp-N endoproteinase (NEB), this part secret thanatins.
mutable notes: This part has 6 PstI sites so you shoud not digest this part by PstI.
mutable provenance: This part is derived from BBa_K759001 and it contains thanatin-Asp fragment that is synthesized by IDT.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: RBS B0034
sequence annotation: Thanatin as alpha domain
sequence annotation: BBa_B0034
sequence annotation: AraC
sequence annotation: double terminator
sequence annotation: BBa_I0500
sequence annotation: PC
sequence annotation: Ag43 beta domain
sequence annotation: Pbad
sequence annotation: Ag43 Signal Peptide
sequence annotation: BBa_B0015
sequence annotation: BBa_K1714000
sequence: https://synbiohub.org/public/igem/BBa_K1714000_sequence/1

RBS B0034
type: SequenceAnnotation
persistent identity: annotation2466879
display id: annotation2466879
version: 1
top level: BBa_K1714000
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1714000/annotation2466879/range2466879/1

https://synbiohub.org/public/igem/BBa_K1714000/annotation2466879/range2466879/1
type: Range
persistent identity: range2466879
display id: range2466879
version: 1
direction: direction/forward
top level: BBa_K1714000
start: 1219
end: 1230
orientation: inline

Thanatin as alpha domain
type: SequenceAnnotation
persistent identity: annotation2466881
display id: annotation2466881
version: 1
top level: BBa_K1714000
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1714000/annotation2466881/range2466881/1

https://synbiohub.org/public/igem/BBa_K1714000/annotation2466881/range2466881/1
type: Range
persistent identity: range2466881
display id: range2466881
version: 1
direction: direction/forward
top level: BBa_K1714000
start: 1402
end: 1467
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2476072
display id: annotation2476072
version: 1
top level: BBa_K1714000
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1714000/annotation2476072/range2476072/1

https://synbiohub.org/public/igem/BBa_K1714000/annotation2476072/range2476072/1
type: Range
persistent identity: range2476072
display id: range2476072
version: 1
direction: direction/forward
top level: BBa_K1714000
start: 1219
end: 1230
orientation: inline

AraC
type: SequenceAnnotation
persistent identity: annotation2466876
display id: annotation2466876
version: 1
top level: BBa_K1714000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1714000/annotation2466876/range2466876/1

https://synbiohub.org/public/igem/BBa_K1714000/annotation2466876/range2466876/1
type: Range
persistent identity: range2466876
display id: range2466876
version: 1
direction: direction/forward
top level: BBa_K1714000
start: 1
end: 1079
orientation: inline

double terminator
type: SequenceAnnotation
persistent identity: annotation2466883
display id: annotation2466883
version: 1
top level: BBa_K1714000
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1714000/annotation2466883/range2466883/1

https://synbiohub.org/public/igem/BBa_K1714000/annotation2466883/range2466883/1
type: Range
persistent identity: range2466883
display id: range2466883
version: 1
direction: direction/forward
top level: BBa_K1714000
start: 2946
end: 3074
orientation: inline

BBa_I0500
type: SequenceAnnotation
persistent identity: annotation2466875
display id: annotation2466875
version: 1
top level: BBa_K1714000
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1714000/annotation2466875/range2466875/1

https://synbiohub.org/public/igem/BBa_K1714000/annotation2466875/range2466875/1
type: Range
persistent identity: range2466875
display id: range2466875
version: 1
direction: direction/forward
top level: BBa_K1714000
start: 1
end: 1210
orientation: inline

PC
type: SequenceAnnotation
persistent identity: annotation2466877
display id: annotation2466877
version: 1
top level: BBa_K1714000
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1714000/annotation2466877/range2466877/1

https://synbiohub.org/public/igem/BBa_K1714000/annotation2466877/range2466877/1
type: Range
persistent identity: range2466877
display id: range2466877
version: 1
direction: direction/forward
top level: BBa_K1714000
start: 1043
end: 1114
orientation: inline

Ag43 beta domain
type: SequenceAnnotation
persistent identity: annotation2466882
display id: annotation2466882
version: 1
top level: BBa_K1714000
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1714000/annotation2466882/range2466882/1

https://synbiohub.org/public/igem/BBa_K1714000/annotation2466882/range2466882/1
type: Range
persistent identity: range2466882
display id: range2466882
version: 1
direction: direction/forward
top level: BBa_K1714000
start: 1474
end: 2945
orientation: inline

Pbad
type: SequenceAnnotation
persistent identity: annotation2466878
display id: annotation2466878
version: 1
top level: BBa_K1714000
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1714000/annotation2466878/range2466878/1

https://synbiohub.org/public/igem/BBa_K1714000/annotation2466878/range2466878/1
type: Range
persistent identity: range2466878
display id: range2466878
version: 1
direction: direction/forward
top level: BBa_K1714000
start: 1080
end: 1210
orientation: inline

Ag43 Signal Peptide
type: SequenceAnnotation
persistent identity: annotation2466880
display id: annotation2466880
version: 1
top level: BBa_K1714000
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1714000/annotation2466880/range2466880/1

https://synbiohub.org/public/igem/BBa_K1714000/annotation2466880/range2466880/1
type: Range
persistent identity: range2466880
display id: range2466880
version: 1
direction: direction/forward
top level: BBa_K1714000
start: 1237
end: 1395
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2476074
display id: annotation2476074
version: 1
top level: BBa_K1714000
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1714000/annotation2476074/range2476074/1

https://synbiohub.org/public/igem/BBa_K1714000/annotation2476074/range2476074/1
type: Range
persistent identity: range2476074
display id: range2476074
version: 1
direction: direction/forward
top level: BBa_K1714000
start: 2946
end: 3074
orientation: inline

BBa_K1714000
type: SequenceAnnotation
persistent identity: annotation2466874
display id: annotation2466874
version: 1
top level: BBa_K1714000
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1714000/annotation2466874/range2466874/1

https://synbiohub.org/public/igem/BBa_K1714000/annotation2466874/range2466874/1
type: Range
persistent identity: range2466874
display id: range2466874
version: 1
direction: direction/forward
top level: BBa_K1714000
start: 1
end: 3074
orientation: inline

Yeb-Art175
type: ComponentDefinition
persistent identity: BBa K1659003
display id: BBa_K1659003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Artilysin Art-175 fused at N-terminal with porin-dependent YebF secretion signal protein
creator: Wei Chung Kong
created: 2015-08-27T11:00:00Z
modified: 2016-02-01T12:52:17Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2077_
m user id: 4206
owner id: 24524
owning group id: 9
status: status/Planning
bookmark: false
mutable description: to be updated
mutable notes: to be updated
mutable provenance: to be updated
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: stop
sequence annotation: YebF
sequence annotation: Hisx6
sequence annotation: Art-175
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_K1659003_sequence/1

stop
type: SequenceAnnotation
persistent identity: annotation2445829
display id: annotation2445829
version: 1
top level: Yeb-Art175
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1659003/annotation2445829/range2445829/1

https://synbiohub.org/public/igem/BBa_K1659003/annotation2445829/range2445829/1
type: Range
persistent identity: range2445829
display id: range2445829
version: 1
direction: direction/forward
top level: Yeb-Art175
start: 1240
end: 1245
orientation: inline

YebF
type: SequenceAnnotation
persistent identity: annotation2445826
display id: annotation2445826
version: 1
top level: Yeb-Art175
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1659003/annotation2445826/range2445826/1

https://synbiohub.org/public/igem/BBa_K1659003/annotation2445826/range2445826/1
type: Range
persistent identity: range2445826
display id: range2445826
version: 1
direction: direction/forward
top level: Yeb-Art175
start: 4
end: 354
orientation: inline

Hisx6
type: SequenceAnnotation
persistent identity: annotation2445828
display id: annotation2445828
version: 1
top level: Yeb-Art175
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1659003/annotation2445828/range2445828/1

https://synbiohub.org/public/igem/BBa_K1659003/annotation2445828/range2445828/1
type: Range
persistent identity: range2445828
display id: range2445828
version: 1
direction: direction/forward
top level: Yeb-Art175
start: 1222
end: 1239
orientation: inline

Art-175
type: SequenceAnnotation
persistent identity: annotation2445827
display id: annotation2445827
version: 1
top level: Yeb-Art175
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1659003/annotation2445827/range2445827/1

https://synbiohub.org/public/igem/BBa_K1659003/annotation2445827/range2445827/1
type: Range
persistent identity: range2445827
display id: range2445827
version: 1
direction: direction/forward
top level: Yeb-Art175
start: 355
end: 1221
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2445825
display id: annotation2445825
version: 1
top level: Yeb-Art175
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1659003/annotation2445825/range2445825/1

https://synbiohub.org/public/igem/BBa_K1659003/annotation2445825/range2445825/1
type: Range
persistent identity: range2445825
display id: range2445825
version: 1
direction: direction/forward
top level: Yeb-Art175
start: 1
end: 3
orientation: inline

BBa_K1727007
type: ComponentDefinition
persistent identity: BBa K1727007
display id: BBa_K1727007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: T5+Lac Operator+RBS+signal peptide+Epinecidin-1+Terminater
creator: Ying Kuan
created: 2015-09-04T11:00:00Z
modified: 2016-02-01T01:24:01Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2148_
m user id: 4206
owner id: 25489
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Antimicrobial peptides (AMPs) are stable peptides that have extensive ability to kill or inhibit the growth of bacteria. They play a role in defense mechanism for all organisms, ranging from prokaryotes to humans, to against predators. Some of them act against specific strain of microbes. Epinecidin-1 is a kind of antimicrobial peptide. This AMP is extracted from the skin mucus of Epinephelus coioides, respectively. Epinecidin-1 has been shown to help wound healing.We treat a signal peptide in front of Epinecidin-1. This signal peptide comes from S.lividas and has ability to let E.coli secrect signiferin to LB culture medium. We use promoter,Lac operator,RBS,terminator on pQE 60 vector.
mutable notes: No
mutable provenance: Epicidni
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: RBS
sequence annotation: Signal peptide
sequence annotation: Restriction enzyme Bgl II site
sequence annotation: -35
sequence annotation: Epinecidin-1
sequence annotation: Lac operator
sequence annotation: T5 promoter
sequence annotation: Restriction enzyme BamH I site
sequence annotation: Alanine cutting site
sequence annotation: lambda t0 terminator
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_K1727007_sequence/1

RBS
type: SequenceAnnotation
persistent identity: annotation2455826
display id: annotation2455826
version: 1
top level: BBa_K1727007
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1727007/annotation2455826/range2455826/1

https://synbiohub.org/public/igem/BBa_K1727007/annotation2455826/range2455826/1
type: Range
persistent identity: range2455826
display id: range2455826
version: 1
direction: direction/forward
top level: BBa_K1727007
start: 92
end: 97
orientation: inline

Signal peptide
type: SequenceAnnotation
persistent identity: annotation2456167
display id: annotation2456167
version: 1
top level: BBa_K1727007
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1727007/annotation2456167/range2456167/1

https://synbiohub.org/public/igem/BBa_K1727007/annotation2456167/range2456167/1
type: Range
persistent identity: range2456167
display id: range2456167
version: 1
direction: direction/forward
top level: BBa_K1727007
start: 118
end: 207
orientation: inline

Restriction enzyme Bgl II site
type: SequenceAnnotation
persistent identity: annotation2456858
display id: annotation2456858
version: 1
top level: BBa_K1727007
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1727007/annotation2456858/range2456858/1

https://synbiohub.org/public/igem/BBa_K1727007/annotation2456858/range2456858/1
type: Range
persistent identity: range2456858
display id: range2456858
version: 1
direction: direction/forward
top level: BBa_K1727007
start: 415
end: 420
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2454875
display id: annotation2454875
version: 1
top level: BBa_K1727007
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1727007/annotation2454875/range2454875/1

https://synbiohub.org/public/igem/BBa_K1727007/annotation2454875/range2454875/1
type: Range
persistent identity: range2454875
display id: range2454875
version: 1
direction: direction/forward
top level: BBa_K1727007
start: 16
end: 21
orientation: inline

Epinecidin-1
type: SequenceAnnotation
persistent identity: annotation2457046
display id: annotation2457046
version: 1
top level: BBa_K1727007
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1727007/annotation2457046/range2457046/1

https://synbiohub.org/public/igem/BBa_K1727007/annotation2457046/range2457046/1
type: Range
persistent identity: range2457046
display id: range2457046
version: 1
direction: direction/forward
top level: BBa_K1727007
start: 211
end: 414
orientation: inline

Lac operator
type: SequenceAnnotation
persistent identity: annotation2454893
display id: annotation2454893
version: 1
top level: BBa_K1727007
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K1727007/annotation2454893/range2454893/1

https://synbiohub.org/public/igem/BBa_K1727007/annotation2454893/range2454893/1
type: Range
persistent identity: range2454893
display id: range2454893
version: 1
direction: direction/forward
top level: BBa_K1727007
start: 53
end: 69
orientation: inline

T5 promoter
type: SequenceAnnotation
persistent identity: annotation2454874
display id: annotation2454874
version: 1
top level: BBa_K1727007
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1727007/annotation2454874/range2454874/1

https://synbiohub.org/public/igem/BBa_K1727007/annotation2454874/range2454874/1
type: Range
persistent identity: range2454874
display id: range2454874
version: 1
direction: direction/forward
top level: BBa_K1727007
start: 1
end: 45
orientation: inline

Restriction enzyme BamH I site
type: SequenceAnnotation
persistent identity: annotation2456165
display id: annotation2456165
version: 1
top level: BBa_K1727007
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1727007/annotation2456165/range2456165/1

https://synbiohub.org/public/igem/BBa_K1727007/annotation2456165/range2456165/1
type: Range
persistent identity: range2456165
display id: range2456165
version: 1
direction: direction/forward
top level: BBa_K1727007
start: 112
end: 117
orientation: inline

Alanine cutting site
type: SequenceAnnotation
persistent identity: annotation2457052
display id: annotation2457052
version: 1
top level: BBa_K1727007
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1727007/annotation2457052/range2457052/1

https://synbiohub.org/public/igem/BBa_K1727007/annotation2457052/range2457052/1
type: Range
persistent identity: range2457052
display id: range2457052
version: 1
direction: direction/forward
top level: BBa_K1727007
start: 208
end: 210
orientation: inline

lambda t0 terminator
type: SequenceAnnotation
persistent identity: annotation2459612
display id: annotation2459612
version: 1
top level: BBa_K1727007
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1727007/annotation2459612/range2459612/1

https://synbiohub.org/public/igem/BBa_K1727007/annotation2459612/range2459612/1
type: Range
persistent identity: range2459612
display id: range2459612
version: 1
direction: direction/forward
top level: BBa_K1727007
start: 461
end: 555
orientation: inline

Lac operator
type: SequenceAnnotation
persistent identity: annotation2454876
display id: annotation2454876
version: 1
top level: BBa_K1727007
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K1727007/annotation2454876/range2454876/1

https://synbiohub.org/public/igem/BBa_K1727007/annotation2454876/range2454876/1
type: Range
persistent identity: range2454876
display id: range2454876
version: 1
direction: direction/forward
top level: BBa_K1727007
start: 21
end: 37
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2454877
display id: annotation2454877
version: 1
top level: BBa_K1727007
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1727007/annotation2454877/range2454877/1

https://synbiohub.org/public/igem/BBa_K1727007/annotation2454877/range2454877/1
type: Range
persistent identity: range2454877
display id: range2454877
version: 1
direction: direction/forward
top level: BBa_K1727007
start: 39
end: 45
orientation: inline

BBa_K1230011
type: ComponentDefinition
persistent identity: BBa K1230011
display id: BBa_K1230011
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: araC-pBad-rbs-LL37
creator: Andr??s Ferri??o Iriarte
created: 2013-09-24T11:00:00Z
modified: 2016-02-03T10:57:18Z
discontinued: false
dominant: false
group u list: _1544_
m user id: 4206
owner id: 17080
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: LL-37 antimicrobial peptide generator under the Arabinose promoter.
mutable notes: In the absence of arabinose, AraC protein represses the structural gene by binding to araI1 and araO2 and the DNA forms a loop.
mutable provenance: 2013 DNA distribution.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K1230009
component: BBa_K1230001
sequence annotation: BBa_K1230009
sequence annotation: BBa_K1230001
sequence: https://synbiohub.org/public/igem/BBa_K1230011_sequence/1

BBa_K1230009
type: Component
persistent identity: component2362448
display id: component2362448
version: 1
top level: BBa_K1230011
access: public
definition: https://synbiohub.org/public/igem/BBa_K1230009/1

BBa_K1230001
type: Component
persistent identity: component2362452
display id: component2362452
version: 1
top level: BBa_K1230011
access: public
definition: LL-37

BBa_K1230009
type: SequenceAnnotation
persistent identity: annotation2362448
display id: annotation2362448
version: 1
top level: BBa_K1230011
location: https://synbiohub.org/public/igem/BBa_K1230011/annotation2362448/range2362448/1
component: BBa_K1230009

https://synbiohub.org/public/igem/BBa_K1230011/annotation2362448/range2362448/1
type: Range
persistent identity: range2362448
display id: range2362448
version: 1
direction: direction/reverse
top level: BBa_K1230011
start: 1
end: 1229
orientation: inline

BBa_K1230001
type: SequenceAnnotation
persistent identity: annotation2362452
display id: annotation2362452
version: 1
top level: BBa_K1230011
location: https://synbiohub.org/public/igem/BBa_K1230011/annotation2362452/range2362452/1
component: BBa_K1230001

https://synbiohub.org/public/igem/BBa_K1230011/annotation2362452/range2362452/1
type: Range
persistent identity: range2362452
display id: range2362452
version: 1
direction: direction/reverse
top level: BBa_K1230011
start: 1236
end: 1355
orientation: inline

BBa_K1647017
type: ComponentDefinition
persistent identity: BBa K1647017
display id: BBa_K1647017
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Bac8c,a kind of antimicrobial peptides
creator: Yun Xue
created: 2015-09-14T11:00:00Z
modified: 2016-01-28T12:08:17Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2064_
m user id: 4206
owner id: 28136
owning group id: 9
status: status/Planning
bookmark: false
mutable description: The antimicrobial peptide, Bac8c, has a potential clinical application in preventing and treating dental caries. S.mutans treated with Bac8c shows abundance effect on bacterial structure. There appears to be a large amount of extracellular debris and obvious holes on the cell surface, as well as loss of cell wall and nucleoid condensation. What???s more, Bac8c can remarkably reduce the viability of cells in biofilms formed in the BioFlux system.
mutable notes: None
mutable provenance: None for the moment
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1647017_sequence/1

BBa_K1595031
type: ComponentDefinition
persistent identity: BBa K1595031
display id: BBa_K1595031
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: EcnA and EcnB from Escherichia coli str. K-12 substr. DH10B
creator: Jonlin Chen
created: 2015-09-17T11:00:00Z
modified: 2016-02-03T10:54:52Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2012_
m user id: 4206
owner id: 18839
owning group id: 9
status: status/Planning
bookmark: false
mutable description: T7 + RBS + EDA + GSG + NdeI + EcnA + TEV + EcnB + 6xHist tag + BamHI + Terminator. Coding device for stabilizing protein EDA linked to EcnA/EcnB through a GSG linker.
mutable notes: Use in conjunction with strain-expressing T7 polymerase.
mutable provenance: Synthesized by DNA 2.0
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: EcnB
sequence annotation: EcnA
sequence annotation: BamHI cutsite
sequence annotation: Terminator
sequence annotation: TEV cutsite
sequence annotation: T7
sequence annotation: NdeI cutsite
sequence annotation: RBS
sequence annotation: 6xHist tag
sequence: https://synbiohub.org/public/igem/BBa_K1595031_sequence/1

EcnB
type: SequenceAnnotation
persistent identity: annotation2469855
display id: annotation2469855
version: 1
top level: BBa_K1595031
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1595031/annotation2469855/range2469855/1

https://synbiohub.org/public/igem/BBa_K1595031/annotation2469855/range2469855/1
type: Range
persistent identity: range2469855
display id: range2469855
version: 1
direction: direction/forward
top level: BBa_K1595031
start: 228
end: 371
orientation: inline

EcnA
type: SequenceAnnotation
persistent identity: annotation2469857
display id: annotation2469857
version: 1
top level: BBa_K1595031
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1595031/annotation2469857/range2469857/1

https://synbiohub.org/public/igem/BBa_K1595031/annotation2469857/range2469857/1
type: Range
persistent identity: range2469857
display id: range2469857
version: 1
direction: direction/forward
top level: BBa_K1595031
start: 87
end: 206
orientation: inline

BamHI cutsite
type: SequenceAnnotation
persistent identity: annotation2469853
display id: annotation2469853
version: 1
top level: BBa_K1595031
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1595031/annotation2469853/range2469853/1

https://synbiohub.org/public/igem/BBa_K1595031/annotation2469853/range2469853/1
type: Range
persistent identity: range2469853
display id: range2469853
version: 1
direction: direction/forward
top level: BBa_K1595031
start: 390
end: 395
orientation: inline

Terminator
type: SequenceAnnotation
persistent identity: annotation2469852
display id: annotation2469852
version: 1
top level: BBa_K1595031
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1595031/annotation2469852/range2469852/1

https://synbiohub.org/public/igem/BBa_K1595031/annotation2469852/range2469852/1
type: Range
persistent identity: range2469852
display id: range2469852
version: 1
direction: direction/forward
top level: BBa_K1595031
start: 396
end: 438
orientation: inline

TEV cutsite
type: SequenceAnnotation
persistent identity: annotation2469856
display id: annotation2469856
version: 1
top level: BBa_K1595031
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1595031/annotation2469856/range2469856/1

https://synbiohub.org/public/igem/BBa_K1595031/annotation2469856/range2469856/1
type: Range
persistent identity: range2469856
display id: range2469856
version: 1
direction: direction/forward
top level: BBa_K1595031
start: 207
end: 227
orientation: inline

T7
type: SequenceAnnotation
persistent identity: annotation2469860
display id: annotation2469860
version: 1
top level: BBa_K1595031
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1595031/annotation2469860/range2469860/1

https://synbiohub.org/public/igem/BBa_K1595031/annotation2469860/range2469860/1
type: Range
persistent identity: range2469860
display id: range2469860
version: 1
direction: direction/forward
top level: BBa_K1595031
start: 1
end: 21
orientation: inline

NdeI cutsite
type: SequenceAnnotation
persistent identity: annotation2469858
display id: annotation2469858
version: 1
top level: BBa_K1595031
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1595031/annotation2469858/range2469858/1

https://synbiohub.org/public/igem/BBa_K1595031/annotation2469858/range2469858/1
type: Range
persistent identity: range2469858
display id: range2469858
version: 1
direction: direction/forward
top level: BBa_K1595031
start: 81
end: 86
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2469859
display id: annotation2469859
version: 1
top level: BBa_K1595031
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1595031/annotation2469859/range2469859/1

https://synbiohub.org/public/igem/BBa_K1595031/annotation2469859/range2469859/1
type: Range
persistent identity: range2469859
display id: range2469859
version: 1
direction: direction/forward
top level: BBa_K1595031
start: 22
end: 80
orientation: inline

6xHist tag
type: SequenceAnnotation
persistent identity: annotation2469854
display id: annotation2469854
version: 1
top level: BBa_K1595031
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1595031/annotation2469854/range2469854/1

https://synbiohub.org/public/igem/BBa_K1595031/annotation2469854/range2469854/1
type: Range
persistent identity: range2469854
display id: range2469854
version: 1
direction: direction/forward
top level: BBa_K1595031
start: 372
end: 389
orientation: inline

BBa_K1727008
type: ComponentDefinition
persistent identity: BBa K1727008
display id: BBa_K1727008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: T5+Lac Operator+RBS+Signal peptide+Signiferin+Terminater
creator: Ying Kuan
created: 2015-09-04T11:00:00Z
modified: 2016-02-03T10:43:43Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2148_
m user id: 4206
owner id: 25489
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Antimicrobial peptides (AMPs) are stable peptides that have extensive ability to kill or inhibit the growth of bacteria. They play a role in defense mechanism for all organisms, ranging from prokaryotes to humans, to against predators. Some of them act against specific strain of microbes. Signiferin is a kinds of antimicrobial peptide extracted from the skin mucus of Crinia signifera, respectively. It demonstrated effectiveness in killing MRSA. And we had change it's sequence into E.coli codon usage. We treat a signal peptide in front of signiferin. This signal peptide comes from S.lividas and has ability to let E.coli secrect signiferin to LB culture medium. We used promoter,Lac operator,RBS,terminator on pQE60 to made our expression machine worked.
mutable notes: No
mutable provenance: EPi
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: lambda t0 terminator
sequence annotation: Alanine cutting site
sequence annotation: Signiferin
sequence annotation: Signal peptide
sequence annotation: -35
sequence annotation: T5 promoter
sequence annotation: Restriction enzyme Nco I site
sequence annotation: Lac operator
sequence annotation: Restriction enzyme BamH I site
sequence annotation: -10
sequence annotation: 3X stop codon
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_K1727008_sequence/1

lambda t0 terminator
type: SequenceAnnotation
persistent identity: annotation2460185
display id: annotation2460185
version: 1
top level: BBa_K1727008
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1727008/annotation2460185/range2460185/1

https://synbiohub.org/public/igem/BBa_K1727008/annotation2460185/range2460185/1
type: Range
persistent identity: range2460185
display id: range2460185
version: 1
direction: direction/forward
top level: BBa_K1727008
start: 305
end: 399
orientation: inline

Alanine cutting site
type: SequenceAnnotation
persistent identity: annotation2460008
display id: annotation2460008
version: 1
top level: BBa_K1727008
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1727008/annotation2460008/range2460008/1

https://synbiohub.org/public/igem/BBa_K1727008/annotation2460008/range2460008/1
type: Range
persistent identity: range2460008
display id: range2460008
version: 1
direction: direction/forward
top level: BBa_K1727008
start: 196
end: 198
orientation: inline

Signiferin
type: SequenceAnnotation
persistent identity: annotation2460009
display id: annotation2460009
version: 1
top level: BBa_K1727008
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1727008/annotation2460009/range2460009/1

https://synbiohub.org/public/igem/BBa_K1727008/annotation2460009/range2460009/1
type: Range
persistent identity: range2460009
display id: range2460009
version: 1
direction: direction/forward
top level: BBa_K1727008
start: 199
end: 243
orientation: inline

Signal peptide
type: SequenceAnnotation
persistent identity: annotation2459984
display id: annotation2459984
version: 1
top level: BBa_K1727008
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1727008/annotation2459984/range2459984/1

https://synbiohub.org/public/igem/BBa_K1727008/annotation2459984/range2459984/1
type: Range
persistent identity: range2459984
display id: range2459984
version: 1
direction: direction/forward
top level: BBa_K1727008
start: 106
end: 195
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2444149
display id: annotation2444149
version: 1
top level: BBa_K1727008
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1727008/annotation2444149/range2444149/1

https://synbiohub.org/public/igem/BBa_K1727008/annotation2444149/range2444149/1
type: Range
persistent identity: range2444149
display id: range2444149
version: 1
direction: direction/forward
top level: BBa_K1727008
start: 16
end: 21
orientation: inline

T5 promoter
type: SequenceAnnotation
persistent identity: annotation2444148
display id: annotation2444148
version: 1
top level: BBa_K1727008
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1727008/annotation2444148/range2444148/1

https://synbiohub.org/public/igem/BBa_K1727008/annotation2444148/range2444148/1
type: Range
persistent identity: range2444148
display id: range2444148
version: 1
direction: direction/forward
top level: BBa_K1727008
start: 1
end: 45
orientation: inline

Restriction enzyme Nco I site
type: SequenceAnnotation
persistent identity: annotation2459629
display id: annotation2459629
version: 1
top level: BBa_K1727008
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1727008/annotation2459629/range2459629/1

https://synbiohub.org/public/igem/BBa_K1727008/annotation2459629/range2459629/1
type: Range
persistent identity: range2459629
display id: range2459629
version: 1
direction: direction/forward
top level: BBa_K1727008
start: 104
end: 109
orientation: inline

Lac operator
type: SequenceAnnotation
persistent identity: annotation2444153
display id: annotation2444153
version: 1
top level: BBa_K1727008
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K1727008/annotation2444153/range2444153/1

https://synbiohub.org/public/igem/BBa_K1727008/annotation2444153/range2444153/1
type: Range
persistent identity: range2444153
display id: range2444153
version: 1
direction: direction/forward
top level: BBa_K1727008
start: 21
end: 37
orientation: inline

Restriction enzyme BamH I site
type: SequenceAnnotation
persistent identity: annotation2460073
display id: annotation2460073
version: 1
top level: BBa_K1727008
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1727008/annotation2460073/range2460073/1

https://synbiohub.org/public/igem/BBa_K1727008/annotation2460073/range2460073/1
type: Range
persistent identity: range2460073
display id: range2460073
version: 1
direction: direction/forward
top level: BBa_K1727008
start: 253
end: 258
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2444150
display id: annotation2444150
version: 1
top level: BBa_K1727008
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1727008/annotation2444150/range2444150/1

https://synbiohub.org/public/igem/BBa_K1727008/annotation2444150/range2444150/1
type: Range
persistent identity: range2444150
display id: range2444150
version: 1
direction: direction/forward
top level: BBa_K1727008
start: 39
end: 45
orientation: inline

3X stop codon
type: SequenceAnnotation
persistent identity: annotation2460025
display id: annotation2460025
version: 1
top level: BBa_K1727008
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1727008/annotation2460025/range2460025/1

https://synbiohub.org/public/igem/BBa_K1727008/annotation2460025/range2460025/1
type: Range
persistent identity: range2460025
display id: range2460025
version: 1
direction: direction/forward
top level: BBa_K1727008
start: 244
end: 252
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2444152
display id: annotation2444152
version: 1
top level: BBa_K1727008
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1727008/annotation2444152/range2444152/1

https://synbiohub.org/public/igem/BBa_K1727008/annotation2444152/range2444152/1
type: Range
persistent identity: range2444152
display id: range2444152
version: 1
direction: direction/forward
top level: BBa_K1727008
start: 92
end: 97
orientation: inline

Lac operator
type: SequenceAnnotation
persistent identity: annotation2444151
display id: annotation2444151
version: 1
top level: BBa_K1727008
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K1727008/annotation2444151/range2444151/1

https://synbiohub.org/public/igem/BBa_K1727008/annotation2444151/range2444151/1
type: Range
persistent identity: range2444151
display id: range2444151
version: 1
direction: direction/forward
top level: BBa_K1727008
start: 53
end: 69
orientation: inline

BBa_K1810002
type: ComponentDefinition
persistent identity: BBa K1810002
display id: BBa_K1810002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Melittin Optimized for E. coli with Mcherry
created: 2015-09-12T11:00:00Z
modified: 2016-02-03T10:46:07Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2235_
m user id: 4206
owner id: 26375
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Melittin is a antimicrobial peptide found in bee venom that works to cause cell lysis by forming a pore in the membrane of harmful bacteria. While the 2014 Stony Brook iGEM team was able to successful clone the melittin gene into E. coli, expression was inconsistent. The 2015 Stony Brook iGEM team worked to correct this by optimizing the codons for an E. coli cell. Both the optimized and regular versions of melittin were fused with a mCherry tag in order to quantify differences in expression. With the use of a fluorescence plate reader, a near 100 fold increase in expression for the optimized melittin construct was observed.
mutable notes: TBA
mutable provenance: While the amino acid sequence for melittin was derived from Apis mellifica, the genomic sequence was altered by hand for optimization in E. coli. The mCherry sequence was obtained from
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: BBa_I13453
sequence annotation: BBa_B0030
sequence annotation: mCherry Tag
sequence annotation: Melittin Optimus
sequence annotation: BBa_B1002
sequence: https://synbiohub.org/public/igem/BBa_K1810002_sequence/1

BBa_I13453
type: SequenceAnnotation
persistent identity: annotation2458006
display id: annotation2458006
version: 1
top level: BBa_K1810002
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1810002/annotation2458006/range2458006/1

https://synbiohub.org/public/igem/BBa_K1810002/annotation2458006/range2458006/1
type: Range
persistent identity: range2458006
display id: range2458006
version: 1
direction: direction/forward
top level: BBa_K1810002
start: 1
end: 130
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation2458005
display id: annotation2458005
version: 1
top level: BBa_K1810002
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1810002/annotation2458005/range2458005/1

https://synbiohub.org/public/igem/BBa_K1810002/annotation2458005/range2458005/1
type: Range
persistent identity: range2458005
display id: range2458005
version: 1
direction: direction/forward
top level: BBa_K1810002
start: 131
end: 145
orientation: inline

mCherry Tag
type: SequenceAnnotation
persistent identity: annotation2458003
display id: annotation2458003
version: 1
top level: BBa_K1810002
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1810002/annotation2458003/range2458003/1

https://synbiohub.org/public/igem/BBa_K1810002/annotation2458003/range2458003/1
type: Range
persistent identity: range2458003
display id: range2458003
version: 1
direction: direction/forward
top level: BBa_K1810002
start: 236
end: 958
orientation: inline

Melittin Optimus
type: SequenceAnnotation
persistent identity: annotation2458002
display id: annotation2458002
version: 1
top level: BBa_K1810002
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1810002/annotation2458002/range2458002/1

https://synbiohub.org/public/igem/BBa_K1810002/annotation2458002/range2458002/1
type: Range
persistent identity: range2458002
display id: range2458002
version: 1
direction: direction/forward
top level: BBa_K1810002
start: 155
end: 235
orientation: inline

BBa_B1002
type: SequenceAnnotation
persistent identity: annotation2458004
display id: annotation2458004
version: 1
top level: BBa_K1810002
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1810002/annotation2458004/range2458004/1

https://synbiohub.org/public/igem/BBa_K1810002/annotation2458004/range2458004/1
type: Range
persistent identity: range2458004
display id: range2458004
version: 1
direction: direction/forward
top level: BBa_K1810002
start: 959
end: 992
orientation: inline

BBa_K1394001
type: ComponentDefinition
persistent identity: BBa K1394001
display id: BBa_K1394001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Linear Magainin 1 peptide
creator: Sean Lowe
created: 2014-10-04T11:00:00Z
modified: 2016-02-03T10:54:34Z
discontinued: false
dominant: false
group u list: _1553_1771_
m user id: 4206
owner id: 17889
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This partcodes for their Magainin 1 antimicrobial peptide. It includes a SUMO fusion partner to allow soluble, non-toxic expression of the gene in E. coli.
mutable notes: Because Magainin 1 is normally cytotoxic to E. coli and other bacteria, the SUMO fusion partner is critical to successful expression of the construct. Without SUMO, the antimicrobial peptide would likely lyse the cell membrane.
mutable provenance: The sequence for Magainin 1 was generated by reverse translating the amino acid sequence for the peptide and then codon optimising it. This gene was then synthesised. The SUMO protein fusion partner was derived from the SUMO biobrick introduced by TU Delft 2013.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Magainin I Antimicrobial Peptide
sequence annotation: T7 terminator
sequence annotation: BBa_K525998
sequence annotation: RBS
sequence annotation: BBa_K731721
sequence annotation: HIS-tagged SUMO Protein
sequence annotation: T7 promoter
sequence annotation: B0034
sequence: https://synbiohub.org/public/igem/BBa_K1394001_sequence/1

Magainin I Antimicrobial Peptide
type: SequenceAnnotation
persistent identity: annotation2427945
display id: annotation2427945
version: 1
top level: BBa_K1394001
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1394001/annotation2427945/range2427945/1

https://synbiohub.org/public/igem/BBa_K1394001/annotation2427945/range2427945/1
type: Range
persistent identity: range2427945
display id: range2427945
version: 1
direction: direction/forward
top level: BBa_K1394001
start: 345
end: 419
orientation: inline

T7 terminator
type: SequenceAnnotation
persistent identity: annotation2427946
display id: annotation2427946
version: 1
top level: BBa_K1394001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1394001/annotation2427946/range2427946/1

https://synbiohub.org/public/igem/BBa_K1394001/annotation2427946/range2427946/1
type: Range
persistent identity: range2427946
display id: range2427946
version: 1
direction: direction/forward
top level: BBa_K1394001
start: 420
end: 467
orientation: inline

BBa_K525998
type: SequenceAnnotation
persistent identity: annotation2427836
display id: annotation2427836
version: 1
top level: BBa_K1394001
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1394001/annotation2427836/range2427836/1

https://synbiohub.org/public/igem/BBa_K1394001/annotation2427836/range2427836/1
type: Range
persistent identity: range2427836
display id: range2427836
version: 1
direction: direction/forward
top level: BBa_K1394001
start: 1
end: 32
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2427839
display id: annotation2427839
version: 1
top level: BBa_K1394001
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1394001/annotation2427839/range2427839/1

https://synbiohub.org/public/igem/BBa_K1394001/annotation2427839/range2427839/1
type: Range
persistent identity: range2427839
display id: range2427839
version: 1
direction: direction/forward
top level: BBa_K1394001
start: 24
end: 27
orientation: inline

BBa_K731721
type: SequenceAnnotation
persistent identity: annotation2427943
display id: annotation2427943
version: 1
top level: BBa_K1394001
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1394001/annotation2427943/range2427943/1

https://synbiohub.org/public/igem/BBa_K1394001/annotation2427943/range2427943/1
type: Range
persistent identity: range2427943
display id: range2427943
version: 1
direction: direction/forward
top level: BBa_K1394001
start: 420
end: 467
orientation: inline

HIS-tagged SUMO Protein
type: SequenceAnnotation
persistent identity: annotation2427944
display id: annotation2427944
version: 1
top level: BBa_K1394001
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1394001/annotation2427944/range2427944/1

https://synbiohub.org/public/igem/BBa_K1394001/annotation2427944/range2427944/1
type: Range
persistent identity: range2427944
display id: range2427944
version: 1
direction: direction/forward
top level: BBa_K1394001
start: 33
end: 344
orientation: inline

T7 promoter
type: SequenceAnnotation
persistent identity: annotation2427838
display id: annotation2427838
version: 1
top level: BBa_K1394001
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1394001/annotation2427838/range2427838/1

https://synbiohub.org/public/igem/BBa_K1394001/annotation2427838/range2427838/1
type: Range
persistent identity: range2427838
display id: range2427838
version: 1
direction: direction/forward
top level: BBa_K1394001
start: 1
end: 20
orientation: inline

B0034
type: SequenceAnnotation
persistent identity: annotation2427837
display id: annotation2427837
version: 1
top level: BBa_K1394001
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1394001/annotation2427837/range2427837/1

https://synbiohub.org/public/igem/BBa_K1394001/annotation2427837/range2427837/1
type: Range
persistent identity: range2427837
display id: range2427837
version: 1
direction: direction/forward
top level: BBa_K1394001
start: 21
end: 32
orientation: inline

Art175
type: ComponentDefinition
persistent identity: BBa K1659000
display id: BBa_K1659000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Artilysin Art-175, an antibacterial fusion protein
creator: Wei Chung Kong
created: 2015-08-27T11:00:00Z
modified: 2016-02-01T12:51:21Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2077_
m user id: 4206
owner id: 24524
owning group id: 9
status: status/Planning
bookmark: false
mutable description: to be updated
mutable notes: to be updated
mutable provenance: to be updated
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Stop
sequence annotation: Art-175
sequence annotation: Start
sequence annotation: Mistake
sequence annotation: Hisx6
sequence: https://synbiohub.org/public/igem/BBa_K1659000_sequence/1

Stop
type: SequenceAnnotation
persistent identity: annotation2440113
display id: annotation2440113
version: 1
top level: Art175
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1659000/annotation2440113/range2440113/1

https://synbiohub.org/public/igem/BBa_K1659000/annotation2440113/range2440113/1
type: Range
persistent identity: range2440113
display id: range2440113
version: 1
direction: direction/forward
top level: Art175
start: 892
end: 897
orientation: inline

Art-175
type: SequenceAnnotation
persistent identity: annotation2440111
display id: annotation2440111
version: 1
top level: Art175
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1659000/annotation2440111/range2440111/1

https://synbiohub.org/public/igem/BBa_K1659000/annotation2440111/range2440111/1
type: Range
persistent identity: range2440111
display id: range2440111
version: 1
direction: direction/forward
top level: Art175
start: 7
end: 873
orientation: inline

Start
type: SequenceAnnotation
persistent identity: annotation2440110
display id: annotation2440110
version: 1
top level: Art175
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1659000/annotation2440110/range2440110/1

https://synbiohub.org/public/igem/BBa_K1659000/annotation2440110/range2440110/1
type: Range
persistent identity: range2440110
display id: range2440110
version: 1
direction: direction/forward
top level: Art175
start: 1
end: 3
orientation: inline

Mistake
type: SequenceAnnotation
persistent identity: annotation2456677
display id: annotation2456677
version: 1
top level: Art175
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K1659000/annotation2456677/range2456677/1

https://synbiohub.org/public/igem/BBa_K1659000/annotation2456677/range2456677/1
type: Range
persistent identity: range2456677
display id: range2456677
version: 1
direction: direction/forward
top level: Art175
start: 4
end: 6
orientation: inline

Hisx6
type: SequenceAnnotation
persistent identity: annotation2440112
display id: annotation2440112
version: 1
top level: Art175
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1659000/annotation2440112/range2440112/1

https://synbiohub.org/public/igem/BBa_K1659000/annotation2440112/range2440112/1
type: Range
persistent identity: range2440112
display id: range2440112
version: 1
direction: direction/forward
top level: Art175
start: 874
end: 891
orientation: inline

BBa_K1162005
type: ComponentDefinition
persistent identity: BBa K1162005
display id: BBa_K1162005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Scygonadin antimicrobial peptide from the horseshoe crab (Scylla serrata)
creator: Ryan Putman
created: 2013-09-14T11:00:00Z
modified: 2016-01-28T12:14:31Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1474_
m user id: 4206
owner id: 13965
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: An anionic antimicrobial peptide.
mutable notes: RFC 23. ATG(Met) added to start of coding region.
mutable provenance: Horseshoe Crab (Scylla serrata)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Met
sequence annotation: Scygonadin
sequence: https://synbiohub.org/public/igem/BBa_K1162005_sequence/1

Met
type: SequenceAnnotation
persistent identity: annotation2365933
display id: annotation2365933
version: 1
top level: BBa_K1162005
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1162005/annotation2365933/range2365933/1

https://synbiohub.org/public/igem/BBa_K1162005/annotation2365933/range2365933/1
type: Range
persistent identity: range2365933
display id: range2365933
version: 1
direction: direction/forward
top level: BBa_K1162005
start: 1
end: 3
orientation: inline

Scygonadin
type: SequenceAnnotation
persistent identity: annotation2365934
display id: annotation2365934
version: 1
top level: BBa_K1162005
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1162005/annotation2365934/range2365934/1

https://synbiohub.org/public/igem/BBa_K1162005/annotation2365934/range2365934/1
type: Range
persistent identity: range2365934
display id: range2365934
version: 1
direction: direction/forward
top level: BBa_K1162005
start: 4
end: 309
orientation: inline

BBa_K1810003
type: ComponentDefinition
persistent identity: BBa K1810003
display id: BBa_K1810003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Optimized Melittin
created: 2015-09-14T11:00:00Z
modified: 2016-02-03T10:47:47Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2235_
m user id: 4206
owner id: 26375
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Melittin is an antimicrobial peptide found in the venom of Apis mellifera. It works by aggregating in the membrane of a bacteria cell and forming a pore, ultimately lysing the cell. An original sequence for melittin in the registry can be found in the BBa_K1382000 BioBrick; however, this sequence was taken directly from Apis Mellifera. In this biobrick, the sequence for melittin has been optimized for E. coli.
mutable notes: The sequence was designed to use the codons that are preferred in E. coli.
mutable provenance: The original sequence is taken from Apis mellifera; however, this sequence has been optimized for E. coli.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Melittin
sequence: https://synbiohub.org/public/igem/BBa_K1810003_sequence/1

Melittin
type: SequenceAnnotation
persistent identity: annotation2457058
display id: annotation2457058
version: 1
top level: BBa_K1810003
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1810003/annotation2457058/range2457058/1

https://synbiohub.org/public/igem/BBa_K1810003/annotation2457058/range2457058/1
type: Range
persistent identity: range2457058
display id: range2457058
version: 1
direction: direction/forward
top level: BBa_K1810003
start: 1
end: 90
orientation: inline

BBa_K1769009
type: ComponentDefinition
persistent identity: BBa K1769009
display id: BBa_K1769009
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: T7+RBS+Lm-def
creator: Ho-Tsang Tsai
created: 2015-09-17T11:00:00Z
modified: 2016-02-03T10:05:55Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2191_
m user id: 4206
owner id: 25576
owning group id: 9
status: status/Planning
bookmark: false
mutable description: 123
mutable notes: no
mutable provenance: no
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: T7 promoter
sequence annotation: lm-def
sequence annotation: rbs
sequence: https://synbiohub.org/public/igem/BBa_K1769009_sequence/1

T7 promoter
type: SequenceAnnotation
persistent identity: annotation2471095
display id: annotation2471095
version: 1
top level: BBa_K1769009
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1769009/annotation2471095/range2471095/1

https://synbiohub.org/public/igem/BBa_K1769009/annotation2471095/range2471095/1
type: Range
persistent identity: range2471095
display id: range2471095
version: 1
direction: direction/forward
top level: BBa_K1769009
start: 1
end: 23
orientation: inline

lm-def
type: SequenceAnnotation
persistent identity: annotation2471096
display id: annotation2471096
version: 1
top level: BBa_K1769009
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1769009/annotation2471096/range2471096/1

https://synbiohub.org/public/igem/BBa_K1769009/annotation2471096/range2471096/1
type: Range
persistent identity: range2471096
display id: range2471096
version: 1
direction: direction/forward
top level: BBa_K1769009
start: 41
end: 196
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation2471097
display id: annotation2471097
version: 1
top level: BBa_K1769009
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1769009/annotation2471097/range2471097/1

https://synbiohub.org/public/igem/BBa_K1769009/annotation2471097/range2471097/1
type: Range
persistent identity: range2471097
display id: range2471097
version: 1
direction: direction/forward
top level: BBa_K1769009
start: 28
end: 29
orientation: inline

BBa_K1742007
type: ComponentDefinition
persistent identity: BBa K1742007
display id: BBa_K1742007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Homo sapiens LTF Lactoferrin
creator: Ruben Escriba Piera
created: 2015-09-03T11:00:00Z
modified: 2016-02-03T10:46:57Z
discontinued: false
dominant: false
group u list: _2164_
m user id: 4206
owner id: 26673
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Lactotransferrin is a major iron-binding and multifunctional protein found in exocrine fluids such as breast milk and mucosal secretions. Has antimicrobial activity, which depends on the extracellular cation concentration. Antimicrobial properties include bacteriostasis, which is related to its ability to sequester free iron and thus inhibit microbial growth, as well as direct bactericidal properties leading to the release of lipopolysaccharides from the bacterial outer membrane. Can also prevent bacterial biofilm development in P.aeruginosa infection. Has weak antifungal activity against C.albicans. Has anabolic, differentiating and anti-apoptotic effects on osteoblasts and can also inhibit osteoclastogenesis, possibly playing a role in the regulation of bone growth. Promotes binding of species C adenoviruses to epithelial cells, promoting adenovirus infection. Can inhibit papillomavirus infections. Stimulates the TLR4 signaling pathway leading to NF-kappa-B activation and subsequent pro-inflammatory cytokine production while also interfering with the lipopolysaccharide (LPS)-stimulated TLR4 signaling. Inhibits neutrophil granulocyte migration to sites of apoptosis, when secreted by apoptotic cells. Stimulates VEGFA-mediated endothelial cell migration and proliferation. Binds heparin, chondroitin sulfate and possibly other glycosaminoglycans (GAGs). Also binds specifically to pneumococcal surface protein A (pspA), the lipid A portion of bacterial lipopolysaccharide (LPS), lysozyme and DNA. Lactoferricin binds to the bacterial surface and is crucial for the bactericidal functions. Has some antiviral activity against papillomavirus infection. N-terminal region shows strong antifungal activity against C.albicans. Contains two BBXB heparin-binding consensus sequences that appear to form the predominate functional GAG-binding site.
mutable notes: Due to its large sequence, we had to designed in two different gBlocks.
mutable provenance: This part was fully synthetized according to the LTF protein (UniprotKB P02788) or/and the corresponding genomic region (GenBank M73700.1; GI:619784)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1742007_sequence/1

LL-37
type: ComponentDefinition
persistent identity: BBa K1230001
display id: BBa_K1230001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LL-37 antimicrobial peptide
creator: Andr??s Ferri??o Iriarte
created: 2013-09-07T11:00:00Z
modified: 2016-02-01T12:49:22Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1544_
m user id: 4206
owner id: 17080
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: The human peptide LL-37 is a cationic antimicrobial peptide with activity against Gram-negative and Gram-positive bacteria. It has been shown to protect against Helicobacter pylori infection and its related gastritis, besides of inhibiting the growth of other pathogenic bacteria in the gastrointestinal tract such as Salmonella typhimurium and Escherichia coli.
mutable notes: Addition of initiation and stop codons.
mutable provenance: Synthesis from genscript
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: Stop
sequence annotation: LL-37
sequence annotation: Start
sequence: https://synbiohub.org/public/igem/BBa_K1230001_sequence/1

Stop
type: SequenceAnnotation
persistent identity: annotation2335371
display id: annotation2335371
version: 1
top level: LL-37
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1230001/annotation2335371/range2335371/1

https://synbiohub.org/public/igem/BBa_K1230001/annotation2335371/range2335371/1
type: Range
persistent identity: range2335371
display id: range2335371
version: 1
direction: direction/forward
top level: LL-37
start: 114
end: 120
orientation: inline

LL-37
type: SequenceAnnotation
persistent identity: annotation2335369
display id: annotation2335369
version: 1
top level: LL-37
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1230001/annotation2335369/range2335369/1

https://synbiohub.org/public/igem/BBa_K1230001/annotation2335369/range2335369/1
type: Range
persistent identity: range2335369
display id: range2335369
version: 1
direction: direction/forward
top level: LL-37
start: 1
end: 120
orientation: inline

Start
type: SequenceAnnotation
persistent identity: annotation2335370
display id: annotation2335370
version: 1
top level: LL-37
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1230001/annotation2335370/range2335370/1

https://synbiohub.org/public/igem/BBa_K1230001/annotation2335370/range2335370/1
type: Range
persistent identity: range2335370
display id: range2335370
version: 1
direction: direction/forward
top level: LL-37
start: 1
end: 3
orientation: inline

BBa_K1162003
type: ComponentDefinition
persistent identity: BBa K1162003
display id: BBa_K1162003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Grammistin-Pp1 antimicrobial peptide from the Clown Grouper (Pogonoperca punctata)
creator: Charles Barentine
created: 2013-09-14T11:00:00Z
modified: 2016-01-28T12:13:42Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1474_
m user id: 4206
owner id: 10131
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: RFC 23 compatible. Contains ATG (methionine).
mutable notes: Codon optimized for E. Coli, contains ATG (met).
mutable provenance: Clown Grouper Fish
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Grammistin-Pp1
sequence annotation: Met
sequence: https://synbiohub.org/public/igem/BBa_K1162003_sequence/1

Grammistin-Pp1
type: SequenceAnnotation
persistent identity: annotation2365795
display id: annotation2365795
version: 1
top level: BBa_K1162003
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1162003/annotation2365795/range2365795/1

https://synbiohub.org/public/igem/BBa_K1162003/annotation2365795/range2365795/1
type: Range
persistent identity: range2365795
display id: range2365795
version: 1
direction: direction/forward
top level: BBa_K1162003
start: 4
end: 42
orientation: inline

Met
type: SequenceAnnotation
persistent identity: annotation2343334
display id: annotation2343334
version: 1
top level: BBa_K1162003
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1162003/annotation2343334/range2343334/1

https://synbiohub.org/public/igem/BBa_K1162003/annotation2343334/range2343334/1
type: Range
persistent identity: range2343334
display id: range2343334
version: 1
direction: direction/forward
top level: BBa_K1162003
start: 1
end: 3
orientation: inline

BBa_K1647018
type: ComponentDefinition
persistent identity: BBa K1647018
display id: BBa_K1647018
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CAP-Bac8c, an improved antimicrobial peptide selectively
creator: Yun Xue
created: 2015-09-14T11:00:00Z
modified: 2016-02-01T12:50:26Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2064_
m user id: 4206
owner id: 28136
owning group id: 9
status: status/Planning
bookmark: false
mutable description: The CAP is a short peptide derived from the competence stimulation peptide(CSP) which can binding to the receptors on the membrane of the S.mutans selectively. The Bac8c is the strong antimicrobial peptide which can kill the bacterial effectively. The part is a new improved peptide ,based on the fusion of a S.mutans-specific targeting peptide domain with a wide-spectrum antimicrobial peptide domain ,which can restrain even kill the S.mutans specifically.
mutable notes: To reduce the effect of the CAP on the Bac8c, the CAP only has the least effective sequence of the CSP. Besides, we design an intersequence ,GGG, put between the CAP and Bac8c for the same goal.
mutable provenance: None for the moment
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1647017
component: BBa_K1647016
sequence annotation: BBa_K1647016
sequence annotation: BBa_K1647017
sequence: https://synbiohub.org/public/igem/BBa_K1647018_sequence/1

BBa_K1647017
type: Component
persistent identity: component2458054
display id: component2458054
version: 1
top level: BBa_K1647018
access: public
definition: BBa_K1647017

BBa_K1647016
type: Component
persistent identity: component2458053
display id: component2458053
version: 1
top level: BBa_K1647018
access: public
definition: https://synbiohub.org/public/igem/BBa_K1647016/1

BBa_K1647016
type: SequenceAnnotation
persistent identity: annotation2458053
display id: annotation2458053
version: 1
top level: BBa_K1647018
location: https://synbiohub.org/public/igem/BBa_K1647018/annotation2458053/range2458053/1
component: BBa_K1647016

https://synbiohub.org/public/igem/BBa_K1647018/annotation2458053/range2458053/1
type: Range
persistent identity: range2458053
display id: range2458053
version: 1
direction: direction/reverse
top level: BBa_K1647018
start: 1
end: 28
orientation: inline

BBa_K1647017
type: SequenceAnnotation
persistent identity: annotation2458054
display id: annotation2458054
version: 1
top level: BBa_K1647018
location: https://synbiohub.org/public/igem/BBa_K1647018/annotation2458054/range2458054/1
component: BBa_K1647017

https://synbiohub.org/public/igem/BBa_K1647018/annotation2458054/range2458054/1
type: Range
persistent identity: range2458054
display id: range2458054
version: 1
direction: direction/reverse
top level: BBa_K1647018
start: 35
end: 64
orientation: inline

BBa_K1364019
type: ComponentDefinition
persistent identity: BBa K1364019
display id: BBa_K1364019
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Pveg + RBS + Antifungal EcAMP + Double terminator
creator: Mathieu Fourni??
created: 2014-10-07T11:00:00Z
modified: 2016-01-28T12:10:42Z
discontinued: false
dominant: false
group u list: _1740_
m user id: 4206
owner id: 21468
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: blabla
mutable notes: bla
mutable provenance: bla
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: K1364011
sequence annotation: STOP
sequence: https://synbiohub.org/public/igem/BBa_K1364019_sequence/1

K1364011
type: SequenceAnnotation
persistent identity: annotation2424706
display id: annotation2424706
version: 1
top level: BBa_K1364019
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1364019/annotation2424706/range2424706/1

https://synbiohub.org/public/igem/BBa_K1364019/annotation2424706/range2424706/1
type: Range
persistent identity: range2424706
display id: range2424706
version: 1
direction: direction/forward
top level: BBa_K1364019
start: 1
end: 366
orientation: inline

STOP
type: SequenceAnnotation
persistent identity: annotation2424708
display id: annotation2424708
version: 1
top level: BBa_K1364019
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1364019/annotation2424708/range2424708/1

https://synbiohub.org/public/igem/BBa_K1364019/annotation2424708/range2424708/1
type: Range
persistent identity: range2424708
display id: range2424708
version: 1
direction: direction/forward
top level: BBa_K1364019
start: 367
end: 369
orientation: inline

Dsb-Art175
type: ComponentDefinition
persistent identity: BBa K1659002
display id: BBa_K1659002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Artilysin Art-175 fused at N-terminal with DsbA signal peptide
creator: Wei Chung Kong
created: 2015-08-25T11:00:00Z
modified: 2016-02-01T12:52:09Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2077_
m user id: 4206
owner id: 24524
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Artilysins are an exciting class of enzyme-based antibacterials. Their name is derived from "artificial endolysin" and they exploit the lytic power of bacteriophage-encoded endolysins. Endolysins are peptidoglycan hydrolases produced at the end of the lytic cycle that pass through the cytoplasmic membrane, degrade the peptidoglycan layer and cause the osmotic lysis of the infected bacterial cell, thus liberating the progeny. Purified endolysins have been used to kill Gram-positive pathogens. Gram-negative bacteria, however, have a protective outer membrane containing lipopolysaccharide (LPS) that serves as a barrier against the peptidoglycan hydrolytic activity of endolysins from the outside. To overcome this problem, selected polycationic or amphipathic peptides that locally destabilize the LPS layer have been covalently fused to endolysins. Biers et al. fused the sheep myeloid antimicrobial peptide (SMAP-29) to the N terminus of the endolysin KZ144 to create Artilysin Art-175. Art-175 has been shown to be a highly potent antibacterial that acts in minutes to kill virtually all Pseudomonas aeruginosa strains. [2] BBa_K1659002 is a composite of Art-175 with the N-terminal DsbA signal peptide with a hexahistidine tag. DsbA targets recombinant proteins to the co-translational signal- recognition-particle (SRP)-dependent pathway to mediate secretion out of the bacterial chassis. [2]
mutable notes: -
mutable provenance: Endolysin KZ144 originates from Pseudomonas aeruginosa bacteriophage varphiKZ. SMAP-29 originates from sheep genomic DNA.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: Hisx6
sequence annotation: DsbA 2-19 Signal Sequence
sequence annotation: stop
sequence annotation: start
sequence annotation: Art-175
sequence: https://synbiohub.org/public/igem/BBa_K1659002_sequence/1

Hisx6
type: SequenceAnnotation
persistent identity: annotation2445688
display id: annotation2445688
version: 1
top level: Dsb-Art175
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1659002/annotation2445688/range2445688/1

https://synbiohub.org/public/igem/BBa_K1659002/annotation2445688/range2445688/1
type: Range
persistent identity: range2445688
display id: range2445688
version: 1
direction: direction/forward
top level: Dsb-Art175
start: 925
end: 942
orientation: inline

DsbA 2-19 Signal Sequence
type: SequenceAnnotation
persistent identity: annotation2445686
display id: annotation2445686
version: 1
top level: Dsb-Art175
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1659002/annotation2445686/range2445686/1

https://synbiohub.org/public/igem/BBa_K1659002/annotation2445686/range2445686/1
type: Range
persistent identity: range2445686
display id: range2445686
version: 1
direction: direction/forward
top level: Dsb-Art175
start: 4
end: 57
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2445689
display id: annotation2445689
version: 1
top level: Dsb-Art175
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1659002/annotation2445689/range2445689/1

https://synbiohub.org/public/igem/BBa_K1659002/annotation2445689/range2445689/1
type: Range
persistent identity: range2445689
display id: range2445689
version: 1
direction: direction/forward
top level: Dsb-Art175
start: 943
end: 948
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2445685
display id: annotation2445685
version: 1
top level: Dsb-Art175
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1659002/annotation2445685/range2445685/1

https://synbiohub.org/public/igem/BBa_K1659002/annotation2445685/range2445685/1
type: Range
persistent identity: range2445685
display id: range2445685
version: 1
direction: direction/forward
top level: Dsb-Art175
start: 1
end: 3
orientation: inline

Art-175
type: SequenceAnnotation
persistent identity: annotation2445687
display id: annotation2445687
version: 1
top level: Dsb-Art175
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1659002/annotation2445687/range2445687/1

https://synbiohub.org/public/igem/BBa_K1659002/annotation2445687/range2445687/1
type: Range
persistent identity: range2445687
display id: range2445687
version: 1
direction: direction/forward
top level: Dsb-Art175
start: 58
end: 924
orientation: inline

D4E1
type: ComponentDefinition
persistent identity: BBa K1364003
display id: BBa_K1364003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS - Antifungal D4E1 - Double terminator
creator: Jourdan Camille
created: 2014-09-24T11:00:00Z
modified: 2016-02-03T10:04:36Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1740_
m user id: 4206
owner id: 21465
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: D4E1 is a linear synthetic peptide of 17 amino acids which has shown to have antifungal activities by complexing with a sterol present in conidial wall of a variety of fungi. This part is composed of a RBS (K780002 spoVG), the open reading frame of D4E1 and a double terminator (B0015). This part was optimized for the expression and its secretion in Bacillus subtilis.
mutable notes: The coding sequence of D4E1 is flanked on the N-terminal end with a signal peptide (amyE signal peptide) followed by a pro peptide, cleaved during the secretion process.
mutable provenance: De Lucca AJ,??Bland JM,??Grimm C,??Jacks TJ,??Cary JW,??Jaynes JM,??Cleveland TE,??Walsh TJ. Fungicidal properties, sterol binding, and proteolytic resistance of the synthetic peptide D4E1. Can J Microbiol.??1998 Jun;44(6):514-20.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
sequence annotation: D4E1
sequence annotation: BBa_K780002 strong RBS
sequence annotation: BBa_B0015
sequence annotation: Signal Peptide
sequence annotation: Pro Peptide
sequence: https://synbiohub.org/public/igem/BBa_K1364003_sequence/1

D4E1
type: SequenceAnnotation
persistent identity: annotation2386386
display id: annotation2386386
version: 1
top level: D4E1
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1364003/annotation2386386/range2386386/1

https://synbiohub.org/public/igem/BBa_K1364003/annotation2386386/range2386386/1
type: Range
persistent identity: range2386386
display id: range2386386
version: 1
direction: direction/forward
top level: D4E1
start: 140
end: 192
orientation: inline

BBa_K780002 strong RBS
type: SequenceAnnotation
persistent identity: annotation2386383
display id: annotation2386383
version: 1
top level: D4E1
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1364003/annotation2386383/range2386383/1

https://synbiohub.org/public/igem/BBa_K1364003/annotation2386383/range2386383/1
type: Range
persistent identity: range2386383
display id: range2386383
version: 1
direction: direction/reverse
top level: D4E1
start: 1
end: 16
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2386387
display id: annotation2386387
version: 1
top level: D4E1
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1364003/annotation2386387/range2386387/1

https://synbiohub.org/public/igem/BBa_K1364003/annotation2386387/range2386387/1
type: Range
persistent identity: range2386387
display id: range2386387
version: 1
direction: direction/forward
top level: D4E1
start: 194
end: 322
orientation: inline

Signal Peptide
type: SequenceAnnotation
persistent identity: annotation2386384
display id: annotation2386384
version: 1
top level: D4E1
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1364003/annotation2386384/range2386384/1

https://synbiohub.org/public/igem/BBa_K1364003/annotation2386384/range2386384/1
type: Range
persistent identity: range2386384
display id: range2386384
version: 1
direction: direction/forward
top level: D4E1
start: 17
end: 97
orientation: inline

Pro Peptide
type: SequenceAnnotation
persistent identity: annotation2386385
display id: annotation2386385
version: 1
top level: D4E1
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1364003/annotation2386385/range2386385/1

https://synbiohub.org/public/igem/BBa_K1364003/annotation2386385/range2386385/1
type: Range
persistent identity: range2386385
display id: range2386385
version: 1
direction: direction/forward
top level: D4E1
start: 98
end: 139
orientation: inline

BBa_K802006
type: ComponentDefinition
persistent identity: BBa K802006
display id: BBa_K802006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Surfactin generator for B. subtilis
creator: Ioana Sandu, Ga??l Chambonnier
created: 2012-09-24T11:00:00Z
modified: 2016-02-01T01:03:57Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1058_
m user id: 4206
owner id: 13266
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: In B. subtilis strains this part can induce the synthesis of surfactin, a lipopeptide with an antimicrobial activity and a powerful surfactant.
mutable notes: This part contains an Bacillus xylose inducible promoter, a RBS for Bacillus and the sfp gene. It can be used to induce surfactin production in Bacillus subtilis strains. However, the sfp gene does not code for the surfactin; it codes for an enzyme which catalyzes the synthesis of the lipoprotein surfactin. In order to overinduce the sfp production and therefore the surfactin synthesis in B. subtilis strains the culture media should be supplemented with xylose.
mutable provenance: The sfp gene was isolated from many B. subtilis strains (for example ATCC 21332).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K802006_sequence/1

BBa_K802009
type: ComponentDefinition
persistent identity: BBa K802009
display id: BBa_K802009
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Sufactin generator and biofilm repressor for B. subtilis
creator: Ioana Sandu, Gael Chambonnier, Alex Mizgier, Marion Traouan
created: 2012-09-24T11:00:00Z
modified: 2016-02-12T09:05:33Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1058_
m user id: 4206
owner id: 13266
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part can be used to induce surfactin production (an antimicrobial lipopeptide) and to repress the biofilm formation in B. subtilis strains.
mutable notes: The part has 3 genes (sfp, abrB and lacI) under the control of the xylose inducible Pxyl promoter.
mutable provenance: The part was constructed from the assembly of 3 biobricks: BBa_K802006,BBa_K802007 and BBa_K802008.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_K802006
component: BBa_K802008
component: BBa_K802007
sequence annotation: BBa_K802007
sequence annotation: BBa_K802008
sequence annotation: BBa_K802006
sequence: https://synbiohub.org/public/igem/BBa_K802009_sequence/1

BBa_K802006
type: Component
persistent identity: component2198693
display id: component2198693
version: 1
top level: BBa_K802009
access: public
definition: BBa_K802006

BBa_K802008
type: Component
persistent identity: component2198695
display id: component2198695
version: 1
top level: BBa_K802009
access: public
definition: https://synbiohub.org/public/igem/BBa_K802008/1

BBa_K802007
type: Component
persistent identity: component2198694
display id: component2198694
version: 1
top level: BBa_K802009
access: public
definition: https://synbiohub.org/public/igem/BBa_K802007/1

BBa_K802007
type: SequenceAnnotation
persistent identity: annotation2198694
display id: annotation2198694
version: 1
top level: BBa_K802009
location: https://synbiohub.org/public/igem/BBa_K802009/annotation2198694/range2198694/1
component: BBa_K802007

https://synbiohub.org/public/igem/BBa_K802009/annotation2198694/range2198694/1
type: Range
persistent identity: range2198694
display id: range2198694
version: 1
direction: direction/reverse
top level: BBa_K802009
start: 796
end: 1099
orientation: inline

BBa_K802008
type: SequenceAnnotation
persistent identity: annotation2198695
display id: annotation2198695
version: 1
top level: BBa_K802009
location: https://synbiohub.org/public/igem/BBa_K802009/annotation2198695/range2198695/1
component: BBa_K802008

https://synbiohub.org/public/igem/BBa_K802009/annotation2198695/range2198695/1
type: Range
persistent identity: range2198695
display id: range2198695
version: 1
direction: direction/reverse
top level: BBa_K802009
start: 1108
end: 2300
orientation: inline

BBa_K802006
type: SequenceAnnotation
persistent identity: annotation2198693
display id: annotation2198693
version: 1
top level: BBa_K802009
location: https://synbiohub.org/public/igem/BBa_K802009/annotation2198693/range2198693/1
component: BBa_K802006

https://synbiohub.org/public/igem/BBa_K802009/annotation2198693/range2198693/1
type: Range
persistent identity: range2198693
display id: range2198693
version: 1
direction: direction/reverse
top level: BBa_K802009
start: 1
end: 787
orientation: inline

BBa_K1351013
type: ComponentDefinition
persistent identity: BBa K1351013
display id: BBa_K1351013
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: spaS Subtilin (antimicrobial peptide)
creator: Carolin Kobras
created: 2014-10-06T11:00:00Z
modified: 2016-01-27T02:42:29Z
discontinued: false
dominant: false
group u list: _1726_
m user id: 4206
owner id: 20398
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: Subtilin is a lanthionine-containing peptide antibiotic (lantibiotic) active on Gram-positive bacteria. The bactericidal activity of lantibiotics is based on depolarization of energized bacterial cytoplasmic membranes, initiated by the formation of aqueous transmembrane pores. This BioBrick differs in its overhangs from BBa_K1351012.
mutable notes: -
mutable provenance: Bacillus subtilis ATCC6633
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1351013_sequence/1

BBa_K1937007
type: ComponentDefinition
persistent identity: BBa K1937007
display id: BBa_K1937007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: AF-A : operon of antifungal peptides
creator: Oumnia KARIM
created: 2016-10-13T11:00:00Z
modified: 2016-10-17T11:22:43Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2404_
m user id: 21497
owner id: 30403
owning group id: 9
status: status/Planning
bookmark: false
mutable description: (Chassis E.coli, carrier plasmid pSB1C3, part destined for use in Bacillus subtilis) Length: 1006 bp Background: AF-A is a part designed to be combined with AF-B to form an operon of 5 antifungal peptides. The interest of using several antifungals is to have a broad spectrum of fungi target, because each antifungal has its own characteristics.The peptides were designed to be expressed and secreted by Bacillus subtilis. This operon was also initially designed to be expressed under the control of the pNagA or pNagP promoter (BBa_K1937003 and BBa_K1937005 respectively). In spite of several cloning strategies, the AF-B construction was not obtained, likely due to the toxicity of one of the peptides for Bacillus subtilis. This BioBrick is a part developed by the Toulouse 2016 iGEM team (http://2016.igem.org/Team:Toulouse_France)
mutable notes: This part encodes two genes encoding antifungal peptides: a cutted Metchnikowin and the D4E1. We created it with the end of the Metchnikowin gene because we found out that only this part of the gene had the antifungal property(http://www.ebi.ac.uk/interpro/entry/IPR012513). The beginning of the gene translates for a propeptide. This gene comes from drosophila and translates a proline-rich peptide whose expression is immune-inducible. The peptide is active against fungi.The D4E1 gene is synthetic and translates a peptide that complexes with cholesterol, current in non-germinated conidia. This synthetic peptide is more resistant than the natural peptide CecroprineA because proteases from fungi have difficulties to degrade the synthetic one (http://www.nrcresearchpress.com/doi/abs/10.1139/w98-032?journalCode=cjm#.V_pRgJOLT6Y). So that each antifungal can be secreted out of the cell, it was necessary to add a sequence called AmyE that enables the transport of molecules outside of the cell. As AmyE is used twice in this Biobrick, we modified the AmyE sequences to avoid homologous recombination while keeping the same protein sequence. The NheI restriction site was added after the RBS to allow promoter swapping with biobrick BAAXXXX or BAAXXXX. SacII and SalI restriction site were added to built the full 5 peptides operon.
mutable provenance: This gene comes from drosophila and translates a proline-rich peptide whose expression is immune-inducible. The peptide is active against fungi.The D4E1 gene is synthetic and translates a peptide that complexes with cholesterol, current in non-germinated conidia. This synthetic peptide is more resistant than the natural peptide CecroprineA because proteases from fungi have difficulties to degrade the synthetic one (http://www.nrcresearchpress.com/doi/abs/10.1139/w98-032?journalCode=cjm#.V_pRgJOLT6Y).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: D4E1
sequence annotation: amyE for cutted Metchnikowin
sequence annotation: RBS Metchnikowin
sequence annotation: AmyE for D4E1
sequence annotation: Pveg
sequence annotation: Cutted Metchnikowin
sequence annotation: RBS D4E1
sequence: https://synbiohub.org/public/igem/BBa_K1937007_sequence/1

D4E1
type: SequenceAnnotation
persistent identity: annotation2514491
display id: annotation2514491
version: 1
top level: BBa_K1937007
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1937007/annotation2514491/range2514491/1

https://synbiohub.org/public/igem/BBa_K1937007/annotation2514491/range2514491/1
type: Range
persistent identity: range2514491
display id: range2514491
version: 1
direction: direction/forward
top level: BBa_K1937007
start: 589
end: 648
orientation: inline

amyE for cutted Metchnikowin
type: SequenceAnnotation
persistent identity: annotation2514487
display id: annotation2514487
version: 1
top level: BBa_K1937007
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1937007/annotation2514487/range2514487/1

https://synbiohub.org/public/igem/BBa_K1937007/annotation2514487/range2514487/1
type: Range
persistent identity: range2514487
display id: range2514487
version: 1
direction: direction/forward
top level: BBa_K1937007
start: 253
end: 375
orientation: inline

RBS Metchnikowin
type: SequenceAnnotation
persistent identity: annotation2514486
display id: annotation2514486
version: 1
top level: BBa_K1937007
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1937007/annotation2514486/range2514486/1

https://synbiohub.org/public/igem/BBa_K1937007/annotation2514486/range2514486/1
type: Range
persistent identity: range2514486
display id: range2514486
version: 1
direction: direction/forward
top level: BBa_K1937007
start: 238
end: 249
orientation: inline

AmyE for D4E1
type: SequenceAnnotation
persistent identity: annotation2514490
display id: annotation2514490
version: 1
top level: BBa_K1937007
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1937007/annotation2514490/range2514490/1

https://synbiohub.org/public/igem/BBa_K1937007/annotation2514490/range2514490/1
type: Range
persistent identity: range2514490
display id: range2514490
version: 1
direction: direction/forward
top level: BBa_K1937007
start: 466
end: 588
orientation: inline

Pveg
type: SequenceAnnotation
persistent identity: annotation2514485
display id: annotation2514485
version: 1
top level: BBa_K1937007
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1937007/annotation2514485/range2514485/1

https://synbiohub.org/public/igem/BBa_K1937007/annotation2514485/range2514485/1
type: Range
persistent identity: range2514485
display id: range2514485
version: 1
direction: direction/forward
top level: BBa_K1937007
start: 1
end: 237
orientation: inline

Cutted Metchnikowin
type: SequenceAnnotation
persistent identity: annotation2514488
display id: annotation2514488
version: 1
top level: BBa_K1937007
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1937007/annotation2514488/range2514488/1

https://synbiohub.org/public/igem/BBa_K1937007/annotation2514488/range2514488/1
type: Range
persistent identity: range2514488
display id: range2514488
version: 1
direction: direction/forward
top level: BBa_K1937007
start: 376
end: 453
orientation: inline

RBS D4E1
type: SequenceAnnotation
persistent identity: annotation2514489
display id: annotation2514489
version: 1
top level: BBa_K1937007
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1937007/annotation2514489/range2514489/1

https://synbiohub.org/public/igem/BBa_K1937007/annotation2514489/range2514489/1
type: Range
persistent identity: range2514489
display id: range2514489
version: 1
direction: direction/forward
top level: BBa_K1937007
start: 454
end: 465
orientation: inline

BBa_K1727005
type: ComponentDefinition
persistent identity: BBa K1727005
display id: BBa_K1727005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Signiferin (Crinia signifera)
creator: Ying Kuan
created: 2015-09-04T11:00:00Z
modified: 2016-02-03T10:48:14Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2148_
m user id: 4206
owner id: 25489
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Antimicrobial peptides (AMPs) are stable peptides that have extensive ability to kill or inhibit the growth of bacteria. They play a role in defense mechanism for all organisms, ranging from prokaryotes to humans, to against predators. Some of them act against specific strain of microbes. Signiferin is a kinds of antimicrobial peptide extracted from the skin mucus of Crinia signifera, respectively. It demonstrated effectiveness in killing MRSA. And we had change it's sequence into E.coli codon usage. We treat a signal peptide in front of signiferin. This signal peptide comes from S.lividas and has ability to let E.coli secrect signiferin to LB culture medium. We used Lac operator,RBS,terminator on pQE60 to made our expression machine worked.
mutable notes: PQE
mutable provenance: Signiferin with signal peptide is artificial synthesis.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Signiferin
sequence: https://synbiohub.org/public/igem/BBa_K1727005_sequence/1

Signiferin
type: SequenceAnnotation
persistent identity: annotation2454863
display id: annotation2454863
version: 1
top level: BBa_K1727005
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1727005/annotation2454863/range2454863/1

https://synbiohub.org/public/igem/BBa_K1727005/annotation2454863/range2454863/1
type: Range
persistent identity: range2454863
display id: range2454863
version: 1
direction: direction/forward
top level: BBa_K1727005
start: 1
end: 54
orientation: inline

BBa_K1639007
type: ComponentDefinition
persistent identity: BBa K1639007
display id: BBa_K1639007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: DAMP-Pexiganan
creator: Mustafa Yılmaz
created: 2015-09-12T11:00:00Z
modified: 2016-02-01T12:50:46Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2056_
m user id: 4206
owner id: 20835
owning group id: 9
status: status/Planning
bookmark: true
mutable description: Synthetic 22-amino-acid antimicrobial peptide(AMP) pexiganan, which is a magainin AMP analog isolated from the skin of the African clawed frog.
mutable notes: This part contains an additional BamHI restriction site at beginning of DAMP-Pex sequence and a XhoI restriction site between DAMP-Pex and double terminator(B0010-B0012). Also have a TEV Cleaveage site(ENLYFQS) between DAMP and Pex.
mutable provenance: none. Synthetic.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
sequence annotation: Pexiganan
sequence annotation: RBS
sequence annotation: T7 terminator
sequence annotation: double terminator (B0010-B0012)
sequence annotation: TEV cleveage site
sequence annotation: rrnB T1 terminator
sequence annotation: 4 X DAMP
sequence annotation: lacIq promoter
sequence: https://synbiohub.org/public/igem/BBa_K1639007_sequence/1

Pexiganan
type: SequenceAnnotation
persistent identity: annotation2453170
display id: annotation2453170
version: 1
top level: BBa_K1639007
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1639007/annotation2453170/range2453170/1

https://synbiohub.org/public/igem/BBa_K1639007/annotation2453170/range2453170/1
type: Range
persistent identity: range2453170
display id: range2453170
version: 1
direction: direction/forward
top level: BBa_K1639007
start: 430
end: 501
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2453172
display id: annotation2453172
version: 1
top level: BBa_K1639007
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1639007/annotation2453172/range2453172/1

https://synbiohub.org/public/igem/BBa_K1639007/annotation2453172/range2453172/1
type: Range
persistent identity: range2453172
display id: range2453172
version: 1
direction: direction/forward
top level: BBa_K1639007
start: 85
end: 107
orientation: inline

T7 terminator
type: SequenceAnnotation
persistent identity: annotation2453175
display id: annotation2453175
version: 1
top level: BBa_K1639007
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1639007/annotation2453175/range2453175/1

https://synbiohub.org/public/igem/BBa_K1639007/annotation2453175/range2453175/1
type: Range
persistent identity: range2453175
display id: range2453175
version: 1
direction: direction/forward
top level: BBa_K1639007
start: 642
end: 669
orientation: inline

double terminator (B0010-B0012)
type: SequenceAnnotation
persistent identity: annotation2453173
display id: annotation2453173
version: 1
top level: BBa_K1639007
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1639007/annotation2453173/range2453173/1

https://synbiohub.org/public/igem/BBa_K1639007/annotation2453173/range2453173/1
type: Range
persistent identity: range2453173
display id: range2453173
version: 1
direction: direction/forward
top level: BBa_K1639007
start: 547
end: 675
orientation: inline

TEV cleveage site
type: SequenceAnnotation
persistent identity: annotation2453169
display id: annotation2453169
version: 1
top level: BBa_K1639007
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1639007/annotation2453169/range2453169/1

https://synbiohub.org/public/igem/BBa_K1639007/annotation2453169/range2453169/1
type: Range
persistent identity: range2453169
display id: range2453169
version: 1
direction: direction/forward
top level: BBa_K1639007
start: 409
end: 429
orientation: inline

rrnB T1 terminator
type: SequenceAnnotation
persistent identity: annotation2453174
display id: annotation2453174
version: 1
top level: BBa_K1639007
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1639007/annotation2453174/range2453174/1

https://synbiohub.org/public/igem/BBa_K1639007/annotation2453174/range2453174/1
type: Range
persistent identity: range2453174
display id: range2453174
version: 1
direction: direction/forward
top level: BBa_K1639007
start: 555
end: 626
orientation: inline

4 X DAMP
type: SequenceAnnotation
persistent identity: annotation2453168
display id: annotation2453168
version: 1
top level: BBa_K1639007
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1639007/annotation2453168/range2453168/1

https://synbiohub.org/public/igem/BBa_K1639007/annotation2453168/range2453168/1
type: Range
persistent identity: range2453168
display id: range2453168
version: 1
direction: direction/forward
top level: BBa_K1639007
start: 115
end: 408
orientation: inline

lacIq promoter
type: SequenceAnnotation
persistent identity: annotation2453171
display id: annotation2453171
version: 1
top level: BBa_K1639007
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1639007/annotation2453171/range2453171/1

https://synbiohub.org/public/igem/BBa_K1639007/annotation2453171/range2453171/1
type: Range
persistent identity: range2453171
display id: range2453171
version: 1
direction: direction/forward
top level: BBa_K1639007
start: 1
end: 78
orientation: inline

BBa_K1022112
type: ComponentDefinition
persistent identity: BBa K1022112
display id: BBa_K1022112
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PT7: RBS: Magainin II: His 6
creator: Maithili Krishnan
created: 2013-09-19T11:00:00Z
modified: 2016-02-03T10:57:02Z
discontinued: false
dominant: false
group u list: _1329_
m user id: 4206
owner id: 15691
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This biobrick codes for the promoter pT7, Magainin II peptide with a His 6 tag.
mutable notes: -
mutable provenance: Synthetic
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: His 6
sequence annotation: Magainin II
sequence annotation: pT7
sequence annotation: 34
sequence: https://synbiohub.org/public/igem/BBa_K1022112_sequence/1

His 6
type: SequenceAnnotation
persistent identity: annotation2356250
display id: annotation2356250
version: 1
top level: BBa_K1022112
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1022112/annotation2356250/range2356250/1

https://synbiohub.org/public/igem/BBa_K1022112/annotation2356250/range2356250/1
type: Range
persistent identity: range2356250
display id: range2356250
version: 1
direction: direction/forward
top level: BBa_K1022112
start: 115
end: 145
orientation: inline

Magainin II
type: SequenceAnnotation
persistent identity: annotation2356245
display id: annotation2356245
version: 1
top level: BBa_K1022112
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1022112/annotation2356245/range2356245/1

https://synbiohub.org/public/igem/BBa_K1022112/annotation2356245/range2356245/1
type: Range
persistent identity: range2356245
display id: range2356245
version: 1
direction: direction/forward
top level: BBa_K1022112
start: 49
end: 114
orientation: inline

pT7
type: SequenceAnnotation
persistent identity: annotation2356228
display id: annotation2356228
version: 1
top level: BBa_K1022112
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1022112/annotation2356228/range2356228/1

https://synbiohub.org/public/igem/BBa_K1022112/annotation2356228/range2356228/1
type: Range
persistent identity: range2356228
display id: range2356228
version: 1
direction: direction/forward
top level: BBa_K1022112
start: 1
end: 28
orientation: inline

34
type: SequenceAnnotation
persistent identity: annotation2356242
display id: annotation2356242
version: 1
top level: BBa_K1022112
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1022112/annotation2356242/range2356242/1

https://synbiohub.org/public/igem/BBa_K1022112/annotation2356242/range2356242/1
type: Range
persistent identity: range2356242
display id: range2356242
version: 1
direction: direction/forward
top level: BBa_K1022112
start: 29
end: 48
orientation: inline

BBa_K1162007
type: ComponentDefinition
persistent identity: BBa K1162007
display id: BBa_K1162007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: WAM-1 antimicrobial peptide from the tammar wallaby (Macropus eugenii)
creator: Ryan Putman
created: 2013-09-14T11:00:00Z
modified: 2016-01-28T12:14:14Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1474_
m user id: 4206
owner id: 13965
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: An antimicrobial peptide from the Cathelicidin family of peptides, isolated from the Tammar Wallaby (Macropus eugenii). ATG(Met) added at start of coding region.
mutable notes: RFC 23.
mutable provenance: Tammar Wallaby (Macropus eugenii)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Met
sequence annotation: WAM-1
sequence: https://synbiohub.org/public/igem/BBa_K1162007_sequence/1

Met
type: SequenceAnnotation
persistent identity: annotation2362765
display id: annotation2362765
version: 1
top level: BBa_K1162007
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1162007/annotation2362765/range2362765/1

https://synbiohub.org/public/igem/BBa_K1162007/annotation2362765/range2362765/1
type: Range
persistent identity: range2362765
display id: range2362765
version: 1
direction: direction/forward
top level: BBa_K1162007
start: 1
end: 3
orientation: inline

WAM-1
type: SequenceAnnotation
persistent identity: annotation2362766
display id: annotation2362766
version: 1
top level: BBa_K1162007
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1162007/annotation2362766/range2362766/1

https://synbiohub.org/public/igem/BBa_K1162007/annotation2362766/range2362766/1
type: Range
persistent identity: range2362766
display id: range2362766
version: 1
direction: direction/forward
top level: BBa_K1162007
start: 4
end: 111
orientation: inline

BBa_K1727006
type: ComponentDefinition
persistent identity: BBa K1727006
display id: BBa_K1727006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Lac operator+RBS+Signal peptide+Signiferin+Terminator
creator: Ying Kuan
created: 2015-09-04T11:00:00Z
modified: 2016-02-03T10:05:22Z
discontinued: true
dominant: false
experience: experience/None
group u list: _2148_
m user id: 4206
owner id: 25489
owning group id: 9
status: status/Deleted
bookmark: false
mutable description: Antimicrobial peptides (AMPs) are stable peptides that have extensive ability to kill or inhibit the growth of bacteria. They play a role in defense mechanism for all organisms, ranging from prokaryotes to humans, to against predators. Some of them act against specific strain of microbes. Signiferin is a kinds of antimicrobial peptide extracted from the skin mucus of Crinia signifera, respectively. It demonstrated effectiveness in killing MRSA. And we had change it's sequence into E.coli codon usage. We treat a signal peptide in front of signiferin. This signal peptide comes from S.lividas and has ability to let E.coli secrect signiferin to LB culture medium. We used Lac operator,RBS,terminator on pQE60 to made our expression machine worked.
mutable notes: PQE
mutable provenance: Signiferin with signal peptide is artificial synthesis.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1727000
sequence annotation: BBa_K1727000
sequence: https://synbiohub.org/public/igem/BBa_K1727006_sequence/1

BBa_K1727000
type: Component
persistent identity: component2444073
display id: component2444073
version: 1
top level: BBa_K1727006
access: public
definition: BBa_K1727000

BBa_K1727000
type: SequenceAnnotation
persistent identity: annotation2444073
display id: annotation2444073
version: 1
top level: BBa_K1727006
location: https://synbiohub.org/public/igem/BBa_K1727006/annotation2444073/range2444073/1
component: BBa_K1727000

https://synbiohub.org/public/igem/BBa_K1727006/annotation2444073/range2444073/1
type: Range
persistent identity: range2444073
display id: range2444073
version: 1
direction: direction/reverse
top level: BBa_K1727006
start: 1
end: 179
orientation: inline

BBa_K1162001
type: ComponentDefinition
persistent identity: BBa K1162001
display id: BBa_K1162001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: EcAMP-1 antimicrobial peptide from barnyard grass (Echinochloa crus-galli)
creator: Charles Barentine
created: 2013-09-14T11:00:00Z
modified: 2016-01-28T12:12:04Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1474_
m user id: 4206
owner id: 10131
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: RFC 23 compatible. Contains ATG (methionine).
mutable notes: Codon optimized for E. Coli, contains ATG (met).
mutable provenance: Echinochloa crus-galli seeds.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: EcAMP-1
sequence annotation: Met
sequence: https://synbiohub.org/public/igem/BBa_K1162001_sequence/1

EcAMP-1
type: SequenceAnnotation
persistent identity: annotation2365787
display id: annotation2365787
version: 1
top level: BBa_K1162001
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1162001/annotation2365787/range2365787/1

https://synbiohub.org/public/igem/BBa_K1162001/annotation2365787/range2365787/1
type: Range
persistent identity: range2365787
display id: range2365787
version: 1
direction: direction/forward
top level: BBa_K1162001
start: 4
end: 114
orientation: inline

Met
type: SequenceAnnotation
persistent identity: annotation2343326
display id: annotation2343326
version: 1
top level: BBa_K1162001
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1162001/annotation2343326/range2343326/1

https://synbiohub.org/public/igem/BBa_K1162001/annotation2343326/range2343326/1
type: Range
persistent identity: range2343326
display id: range2343326
version: 1
direction: direction/forward
top level: BBa_K1162001
start: 1
end: 3
orientation: inline

RFC 37
type: ComponentDefinition
persistent identity: BBa K245114
display id: BBa_K245114
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LL37
creator: Nika Debeljak
created: 2009-10-13T11:00:00Z
modified: 2016-01-27T02:41:54Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _337_
m user id: 4206
owner id: 4964
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: x
mutable notes: This part is inserted in BB-NIC-II-HisN vector (BBa_K245005) and can be assembled with other parts according to assembly standard 37.
mutable provenance: the part is of syntethic origin
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K245114_sequence/1

BBa_K1727003
type: ComponentDefinition
persistent identity: BBa K1727003
display id: BBa_K1727003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Epinecidin-1 with signal peptide
creator: Ying Kuan
created: 2015-09-04T11:00:00Z
modified: 2016-02-01T01:23:48Z
discontinued: false
dominant: false
group u list: _2148_
m user id: 4206
owner id: 25489
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Antimicrobial peptides (AMPs) are stable peptides that have extensive ability to kill or inhibit the growth of bacteria. They play a role in defense mechanism for all organisms, ranging from prokaryotes to humans, to against predators. Some of them act against specific strain of microbes. Epinecidin-1 is a kind of antimicrobial peptide. This AMP is extracted from the skin mucus of Epinephelus coioides, respectively. Epinecidin-1 has been shown to help wound healing.We treat a signal peptide in front of Epinecidin-1. This signal peptide comes from S.lividas and has ability to let E.coli secrect signiferin to LB culture medium.
mutable notes: We use PQE60
mutable provenance: Epinecidin-1 is comes from Epinephelus coioides
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Restriction enzyme BamH I site
sequence annotation: Epinecidin-1
sequence annotation: Restriction enzyme Bgl ll site
sequence annotation: Signal peptide
sequence: https://synbiohub.org/public/igem/BBa_K1727003_sequence/1

Restriction enzyme BamH I site
type: SequenceAnnotation
persistent identity: annotation2444074
display id: annotation2444074
version: 1
top level: BBa_K1727003
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1727003/annotation2444074/range2444074/1

https://synbiohub.org/public/igem/BBa_K1727003/annotation2444074/range2444074/1
type: Range
persistent identity: range2444074
display id: range2444074
version: 1
direction: direction/forward
top level: BBa_K1727003
start: 1
end: 6
orientation: inline

Epinecidin-1
type: SequenceAnnotation
persistent identity: annotation2444147
display id: annotation2444147
version: 1
top level: BBa_K1727003
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1727003/annotation2444147/range2444147/1

https://synbiohub.org/public/igem/BBa_K1727003/annotation2444147/range2444147/1
type: Range
persistent identity: range2444147
display id: range2444147
version: 1
direction: direction/forward
top level: BBa_K1727003
start: 100
end: 303
orientation: inline

Restriction enzyme Bgl ll site
type: SequenceAnnotation
persistent identity: annotation2444140
display id: annotation2444140
version: 1
top level: BBa_K1727003
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1727003/annotation2444140/range2444140/1

https://synbiohub.org/public/igem/BBa_K1727003/annotation2444140/range2444140/1
type: Range
persistent identity: range2444140
display id: range2444140
version: 1
direction: direction/forward
top level: BBa_K1727003
start: 304
end: 309
orientation: inline

Signal peptide
type: SequenceAnnotation
persistent identity: annotation2444141
display id: annotation2444141
version: 1
top level: BBa_K1727003
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1727003/annotation2444141/range2444141/1

https://synbiohub.org/public/igem/BBa_K1727003/annotation2444141/range2444141/1
type: Range
persistent identity: range2444141
display id: range2444141
version: 1
direction: direction/forward
top level: BBa_K1727003
start: 7
end: 99
orientation: inline

SboA
type: ComponentDefinition
persistent identity: BBa K1697000
display id: BBa_K1697000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: SboA from B.subtilis 168
creator: UI Indonesia 2015
created: 2015-09-02T11:00:00Z
modified: 2016-02-03T10:03:43Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2115_
m user id: 4206
owner id: 24798
owning group id: 9
status: status/Planning
bookmark: true
mutable description: Subtilosin coding region without its operon
mutable notes: The operon for SboA is not included
mutable provenance: Genomic sequence of Bacillus subtilis 168
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: ATG
sequence annotation: TAA
sequence annotation: SboA
sequence: https://synbiohub.org/public/igem/BBa_K1697000_sequence/1

ATG
type: SequenceAnnotation
persistent identity: annotation2453083
display id: annotation2453083
version: 1
top level: SboA
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1697000/annotation2453083/range2453083/1

https://synbiohub.org/public/igem/BBa_K1697000/annotation2453083/range2453083/1
type: Range
persistent identity: range2453083
display id: range2453083
version: 1
direction: direction/forward
top level: SboA
start: 1
end: 3
orientation: inline

TAA
type: SequenceAnnotation
persistent identity: annotation2453084
display id: annotation2453084
version: 1
top level: SboA
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1697000/annotation2453084/range2453084/1

https://synbiohub.org/public/igem/BBa_K1697000/annotation2453084/range2453084/1
type: Range
persistent identity: range2453084
display id: range2453084
version: 1
direction: direction/forward
top level: SboA
start: 130
end: 132
orientation: inline

SboA
type: SequenceAnnotation
persistent identity: annotation2453085
display id: annotation2453085
version: 1
top level: SboA
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1697000/annotation2453085/range2453085/1

https://synbiohub.org/public/igem/BBa_K1697000/annotation2453085/range2453085/1
type: Range
persistent identity: range2453085
display id: range2453085
version: 1
direction: direction/forward
top level: SboA
start: 1
end: 132
orientation: inline

BBa_K1022102
type: ComponentDefinition
persistent identity: BBa K1022102
display id: BBa_K1022102
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pT7: RBS: His6- SUMO: Signiferin
creator: Maithili Krishnan
created: 2013-09-04T11:00:00Z
modified: 2016-02-03T10:59:33Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1329_
m user id: 4206
owner id: 15691
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: This part codes for the peptide 'Signiferin' tagged with 'His-6-SUMO' molecule. Its production is triggered by IPTG (through pT7 promoter).
mutable notes: -
mutable provenance: Synthetic
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: His6:SUMO
sequence annotation: T7
sequence annotation: Signiferin
sequence annotation: 34
sequence: https://synbiohub.org/public/igem/BBa_K1022102_sequence/1

His6:SUMO
type: SequenceAnnotation
persistent identity: annotation2334996
display id: annotation2334996
version: 1
top level: BBa_K1022102
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1022102/annotation2334996/range2334996/1

https://synbiohub.org/public/igem/BBa_K1022102/annotation2334996/range2334996/1
type: Range
persistent identity: range2334996
display id: range2334996
version: 1
direction: direction/forward
top level: BBa_K1022102
start: 43
end: 363
orientation: inline

T7
type: SequenceAnnotation
persistent identity: annotation2334994
display id: annotation2334994
version: 1
top level: BBa_K1022102
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1022102/annotation2334994/range2334994/1

https://synbiohub.org/public/igem/BBa_K1022102/annotation2334994/range2334994/1
type: Range
persistent identity: range2334994
display id: range2334994
version: 1
direction: direction/forward
top level: BBa_K1022102
start: 1
end: 28
orientation: inline

Signiferin
type: SequenceAnnotation
persistent identity: annotation2334997
display id: annotation2334997
version: 1
top level: BBa_K1022102
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1022102/annotation2334997/range2334997/1

https://synbiohub.org/public/igem/BBa_K1022102/annotation2334997/range2334997/1
type: Range
persistent identity: range2334997
display id: range2334997
version: 1
direction: direction/forward
top level: BBa_K1022102
start: 364
end: 412
orientation: inline

34
type: SequenceAnnotation
persistent identity: annotation2334995
display id: annotation2334995
version: 1
top level: BBa_K1022102
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1022102/annotation2334995/range2334995/1

https://synbiohub.org/public/igem/BBa_K1022102/annotation2334995/range2334995/1
type: Range
persistent identity: range2334995
display id: range2334995
version: 1
direction: direction/forward
top level: BBa_K1022102
start: 29
end: 42
orientation: inline

BBa_K1351043
type: ComponentDefinition
persistent identity: BBa K1351043
display id: BBa_K1351043
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Canibalism toxin SDP of B. subtilis
creator: Philipp Popp & Roman Herzog
created: 2014-10-27T12:00:00Z
modified: 2016-02-03T10:53:35Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1726_
m user id: 4206
owner id: 20046
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Canibalism toxin of B. subtilis with modification parts SdpA and SdpB.
mutable notes: Several restriction enzyme sites had to be changed in the coding sequence in order to full fill the BioBrick standard criteria.
mutable provenance: B. subtilis
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1351043_sequence/1

BBa_K1351012
type: ComponentDefinition
persistent identity: BBa K1351012
display id: BBa_K1351012
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: spaS Subtilin (antimicrobial peptide, Freiburg standard)
creator: Carolin Kobras
created: 2014-09-09T11:00:00Z
modified: 2016-01-27T02:43:09Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1726_
m user id: 4206
owner id: 20398
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: Subtilin Lanthionine-containing peptide antibiotic (lantibiotic) active on Gram-positive bacteria. The bactericidal activity of lantibiotics is based on depolarization of energized bacterial cytoplasmic membranes, initiated by the formation of aqueous transmembrane pores.
mutable notes: -
mutable provenance: Bacillus subtilis ATCC6633
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1351012_sequence/1

BBa_K1835501
type: ComponentDefinition
persistent identity: BBa K1835501
display id: BBa_K1835501
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Tachyplesin-I
creator: Anthony Ciesla
created: 2015-09-17T11:00:00Z
modified: 2016-02-01T12:48:53Z
discontinued: false
dominant: false
group u list: _2261_
m user id: 4206
owner id: 26097
owning group id: 9
status: status/Planning
bookmark: false
mutable description: An anti-microbial peptide in use in horseshoe crabs,
mutable notes: Warning: the part is very small which lowers BioBrick standard assembly yields drastically.
mutable provenance: Tachypleus Tridentitus, synthesized de novo.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1835501_sequence/1

MccS
type: ComponentDefinition
persistent identity: BBa K1659100
display id: BBa_K1659100
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Microcin S, a Class II microcin produced by probiotic E. coli G3/10
creator: Raphaella Hull
created: 2015-08-09T11:00:00Z
modified: 2016-02-03T10:51:08Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2077_
m user id: 4206
owner id: 24525
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Microcins are antibacterial peptides. Microcin S was isolated from Escherichia coli G3/10, a component of the probiotic drug Symbioflor 2. Here it was shown to be responsible for E. coli G3/10's ability to suppress adherence of enteropathogenic E. coli E2348/69. [1] The Microcin S operon is about 4.7 kb in size and is comprised of four genes. This part is the sequencing encoding the microcin itself (mcsS). [1] Zsch??ttig, A., Zimmermann, K., Blom, J., Goesmann, A., P??hlmann, C., & Gunzer, F. (2012). Identification and characterization of microcin S, a new antibacterial peptide produced by probiotic Escherichia coli G3/10. PLoS ONE, 7(3), 1???9. http://doi.org/10.1371/journal.pone.0033351
mutable notes: No specific design considerations
mutable provenance: UniProtKB - H9ZMG7 (H9ZMG7_ECOLX)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: start
sequence annotation: stop
sequence annotation: Microcin S
sequence annotation: Hisx6
sequence: https://synbiohub.org/public/igem/BBa_K1659100_sequence/1

start
type: SequenceAnnotation
persistent identity: annotation2449243
display id: annotation2449243
version: 1
top level: MccS
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1659100/annotation2449243/range2449243/1

https://synbiohub.org/public/igem/BBa_K1659100/annotation2449243/range2449243/1
type: Range
persistent identity: range2449243
display id: range2449243
version: 1
direction: direction/forward
top level: MccS
start: 1
end: 3
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2449246
display id: annotation2449246
version: 1
top level: MccS
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1659100/annotation2449246/range2449246/1

https://synbiohub.org/public/igem/BBa_K1659100/annotation2449246/range2449246/1
type: Range
persistent identity: range2449246
display id: range2449246
version: 1
direction: direction/forward
top level: MccS
start: 379
end: 384
orientation: inline

Microcin S
type: SequenceAnnotation
persistent identity: annotation2449244
display id: annotation2449244
version: 1
top level: MccS
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1659100/annotation2449244/range2449244/1

https://synbiohub.org/public/igem/BBa_K1659100/annotation2449244/range2449244/1
type: Range
persistent identity: range2449244
display id: range2449244
version: 1
direction: direction/forward
top level: MccS
start: 4
end: 360
orientation: inline

Hisx6
type: SequenceAnnotation
persistent identity: annotation2449245
display id: annotation2449245
version: 1
top level: MccS
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1659100/annotation2449245/range2449245/1

https://synbiohub.org/public/igem/BBa_K1659100/annotation2449245/range2449245/1
type: Range
persistent identity: range2449245
display id: range2449245
version: 1
direction: direction/forward
top level: MccS
start: 361
end: 378
orientation: inline

BBa_K1364011
type: ComponentDefinition
persistent identity: BBa K1364011
display id: BBa_K1364011
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Pveg-spoVG + EcAMP-1 + Double terminator
creator: Jourdan Camille
created: 2014-10-02T11:00:00Z
modified: 2016-01-28T12:11:29Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1740_
m user id: 4206
owner id: 21465
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: bla
mutable notes: bla
mutable provenance: bla
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K1364010
component: BBa_B0015
sequence annotation: BBa_K1364010
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_K1364011_sequence/1

BBa_K1364010
type: Component
persistent identity: component2392910
display id: component2392910
version: 1
top level: BBa_K1364011
access: public
definition: https://synbiohub.org/public/igem/BBa_K1364010/1

BBa_B0015
type: Component
persistent identity: component2392917
display id: component2392917
version: 1
top level: BBa_K1364011
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_K1364010
type: SequenceAnnotation
persistent identity: annotation2392910
display id: annotation2392910
version: 1
top level: BBa_K1364011
location: https://synbiohub.org/public/igem/BBa_K1364011/annotation2392910/range2392910/1
component: BBa_K1364010

https://synbiohub.org/public/igem/BBa_K1364011/annotation2392910/range2392910/1
type: Range
persistent identity: range2392910
display id: range2392910
version: 1
direction: direction/reverse
top level: BBa_K1364011
start: 1
end: 229
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2392917
display id: annotation2392917
version: 1
top level: BBa_K1364011
location: https://synbiohub.org/public/igem/BBa_K1364011/annotation2392917/range2392917/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K1364011/annotation2392917/range2392917/1
type: Range
persistent identity: range2392917
display id: range2392917
version: 1
direction: direction/reverse
top level: BBa_K1364011
start: 238
end: 366
orientation: inline

BBa_K1162006
type: ComponentDefinition
persistent identity: BBa K1162006
display id: BBa_K1162006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LL-37 antimicrobial peptide from Humans (Homo sapiens)
creator: Ryan Putman
created: 2013-09-14T11:00:00Z
modified: 2016-01-27T02:42:11Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1474_
m user id: 4206
owner id: 13965
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: This is an antimicrobial peptide (AMP) is commonly found in humans. This part was commercially synthesized and codon optimized for expression in E. coli and is an improvement on LL-37 parts currently in the registry. The prefix and suffix are compatible with RFC 23 (silver fusion) to allow for protein fusions and this part contains an ???atg??? to allow for immediate protein synthesis. Furthermore this LL-37 derivative does not have any stop codons which allows for composite BioBrick Related parts Previously this part has been used by Slovenia iGEM team 2009 (http://parts.igem.org/wiki/index.php?title=Part:BBa_K245114) This part (BBa_K245114) lacks a start codon. Previously this part has been used Trieste iGEM team 2012 http://parts.igem.org/Part:BBa_K875009?title=Part:BBa_K875009 This part, BBa_K875009, has a BioBrick prefix and suffix of RFC 10 so cannot be used for protein fusions, such as in cases of secretion and protein purification. Also has a stop codon which limits what can be added to the C-terminal end (purification tag/secretion tag).
mutable notes: RFC 23. ATG(Met) added to start of coding region.
mutable provenance: Human (Homo sapiens)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Met
sequence annotation: LL-37
sequence: https://synbiohub.org/public/igem/BBa_K1162006_sequence/1

Met
type: SequenceAnnotation
persistent identity: annotation2348651
display id: annotation2348651
version: 1
top level: BBa_K1162006
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1162006/annotation2348651/range2348651/1

https://synbiohub.org/public/igem/BBa_K1162006/annotation2348651/range2348651/1
type: Range
persistent identity: range2348651
display id: range2348651
version: 1
direction: direction/forward
top level: BBa_K1162006
start: 1
end: 3
orientation: inline

LL-37
type: SequenceAnnotation
persistent identity: annotation2365824
display id: annotation2365824
version: 1
top level: BBa_K1162006
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1162006/annotation2365824/range2365824/1

https://synbiohub.org/public/igem/BBa_K1162006/annotation2365824/range2365824/1
type: Range
persistent identity: range2365824
display id: range2365824
version: 1
direction: direction/forward
top level: BBa_K1162006
start: 4
end: 114
orientation: inline

BBa_K1396000
type: ComponentDefinition
persistent identity: BBa K1396000
display id: BBa_K1396000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pSB1C3: 2XStrep_Flagtag_LL-37_Mussel Foot Protein_sfGFP
creator: Ariel Hernandez-Leyva
created: 2014-10-09T11:00:00Z
modified: 2016-02-03T10:06:09Z
discontinued: false
dominant: false
group u list: _1773_
m user id: 4206
owner id: 22908
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: The part is an coding sequence for an anti-microbial peptides linked to a mussel-foot protein-linked to superfolder GFP for localization. The mussel foot protein will anneal to surfaces as a wet glue and the antimicrobial domain is designed to interact with microbial membranes and interfere with membrane stability. In order to use this part you can produce it in a TAG recoded organism simultaneously expressing a Tyrosine supressor or L-DOPA orthogonal translational system. In order to purify you can use the 2X Strep tag and strep column and later cleave with enterokinase to remove the sequence supressing LL-37 antimicrobial action.
mutable notes: We capped the LL-37 Domain which is otherwise toxic to cells with a removable strep tag that can be used for column chromatography. Additionally there are linker units between individual components
mutable provenance: Synthesized in lab
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Mfp 1-5-1
sequence annotation: 2XStrepFlag Tag
sequence annotation: sfGFP
sequence annotation: LL-37
sequence: https://synbiohub.org/public/igem/BBa_K1396000_sequence/1

Mfp 1-5-1
type: SequenceAnnotation
persistent identity: annotation2414630
display id: annotation2414630
version: 1
top level: BBa_K1396000
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1396000/annotation2414630/range2414630/1

https://synbiohub.org/public/igem/BBa_K1396000/annotation2414630/range2414630/1
type: Range
persistent identity: range2414630
display id: range2414630
version: 1
direction: direction/forward
top level: BBa_K1396000
start: 260
end: 591
orientation: inline

2XStrepFlag Tag
type: SequenceAnnotation
persistent identity: annotation2414624
display id: annotation2414624
version: 1
top level: BBa_K1396000
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1396000/annotation2414624/range2414624/1

https://synbiohub.org/public/igem/BBa_K1396000/annotation2414624/range2414624/1
type: Range
persistent identity: range2414624
display id: range2414624
version: 1
direction: direction/forward
top level: BBa_K1396000
start: 2
end: 121
orientation: inline

sfGFP
type: SequenceAnnotation
persistent identity: annotation2414636
display id: annotation2414636
version: 1
top level: BBa_K1396000
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1396000/annotation2414636/range2414636/1

https://synbiohub.org/public/igem/BBa_K1396000/annotation2414636/range2414636/1
type: Range
persistent identity: range2414636
display id: range2414636
version: 1
direction: direction/forward
top level: BBa_K1396000
start: 1034
end: 1701
orientation: inline

LL-37
type: SequenceAnnotation
persistent identity: annotation2414627
display id: annotation2414627
version: 1
top level: BBa_K1396000
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1396000/annotation2414627/range2414627/1

https://synbiohub.org/public/igem/BBa_K1396000/annotation2414627/range2414627/1
type: Range
persistent identity: range2414627
display id: range2414627
version: 1
direction: direction/forward
top level: BBa_K1396000
start: 122
end: 239
orientation: inline

BBa_K628000
type: ComponentDefinition
persistent identity: BBa K628000
display id: BBa_K628000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: codes for protegrin-1 (anti-microbial peptide)
creator: Charles Thompson
created: 2011-09-18T11:00:00Z
modified: 2016-02-01T12:54:06Z
discontinued: false
dominant: false
group u list: _800_
m user id: 4206
owner id: 9738
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is the coding region for protegrin-1.
mutable notes: None.
mutable provenance: This sequence was derived from protegrin-1 found in porcine leukocytes. The website where we found the sequence is:
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K628000_sequence/1

BBa_K1022103
type: ComponentDefinition
persistent identity: BBa K1022103
display id: BBa_K1022103
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pT7: RBS: His6- SUMO: Magainin II
creator: Maithili Krishnan
created: 2013-09-04T11:00:00Z
modified: 2016-02-03T10:59:14Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1329_
m user id: 4206
owner id: 15691
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This part codes for the peptide 'Magainin II' tagged with 'His-6-SUMO' molecule. Its production is triggered by IPTG (through pT7 promoter).
mutable notes: -
mutable provenance: Synthetic
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: His6:SUMO
sequence annotation: Magainin II
sequence annotation: 34
sequence annotation: T7
sequence: https://synbiohub.org/public/igem/BBa_K1022103_sequence/1

His6:SUMO
type: SequenceAnnotation
persistent identity: annotation2335000
display id: annotation2335000
version: 1
top level: BBa_K1022103
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1022103/annotation2335000/range2335000/1

https://synbiohub.org/public/igem/BBa_K1022103/annotation2335000/range2335000/1
type: Range
persistent identity: range2335000
display id: range2335000
version: 1
direction: direction/forward
top level: BBa_K1022103
start: 43
end: 363
orientation: inline

Magainin II
type: SequenceAnnotation
persistent identity: annotation2335001
display id: annotation2335001
version: 1
top level: BBa_K1022103
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1022103/annotation2335001/range2335001/1

https://synbiohub.org/public/igem/BBa_K1022103/annotation2335001/range2335001/1
type: Range
persistent identity: range2335001
display id: range2335001
version: 1
direction: direction/forward
top level: BBa_K1022103
start: 364
end: 436
orientation: inline

34
type: SequenceAnnotation
persistent identity: annotation2334999
display id: annotation2334999
version: 1
top level: BBa_K1022103
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1022103/annotation2334999/range2334999/1

https://synbiohub.org/public/igem/BBa_K1022103/annotation2334999/range2334999/1
type: Range
persistent identity: range2334999
display id: range2334999
version: 1
direction: direction/forward
top level: BBa_K1022103
start: 29
end: 42
orientation: inline

T7
type: SequenceAnnotation
persistent identity: annotation2334998
display id: annotation2334998
version: 1
top level: BBa_K1022103
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1022103/annotation2334998/range2334998/1

https://synbiohub.org/public/igem/BBa_K1022103/annotation2334998/range2334998/1
type: Range
persistent identity: range2334998
display id: range2334998
version: 1
direction: direction/forward
top level: BBa_K1022103
start: 1
end: 28
orientation: inline

BBa_K628006
type: ComponentDefinition
persistent identity: BBa K628006
display id: BBa_K628006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Protegrin-1 Kill Switch
creator: Charles Thompson
created: 2011-09-19T11:00:00Z
modified: 2016-02-01T12:54:32Z
discontinued: false
dominant: false
group u list: _800_
m user id: 4206
owner id: 9738
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: TBA
mutable notes: TBA
mutable provenance: TBA
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K628000
component: BBa_J61101
component: BBa_B0015
component: BBa_K206000
sequence annotation: BBa_K628000
sequence annotation: BBa_B0015
sequence annotation: BBa_J61101
sequence annotation: BBa_K206000
sequence: https://synbiohub.org/public/igem/BBa_K628006_sequence/1

BBa_K628000
type: Component
persistent identity: component2135982
display id: component2135982
version: 1
top level: BBa_K628006
access: public
definition: BBa_K628000

BBa_J61101
type: Component
persistent identity: component2135981
display id: component2135981
version: 1
top level: BBa_K628006
access: public
definition: https://synbiohub.org/public/igem/BBa_J61101/1

BBa_B0015
type: Component
persistent identity: component2135989
display id: component2135989
version: 1
top level: BBa_K628006
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_K206000
type: Component
persistent identity: component2135980
display id: component2135980
version: 1
top level: BBa_K628006
access: public
definition: https://synbiohub.org/public/igem/BBa_K206000/1

BBa_K628000
type: SequenceAnnotation
persistent identity: annotation2135982
display id: annotation2135982
version: 1
top level: BBa_K628006
location: https://synbiohub.org/public/igem/BBa_K628006/annotation2135982/range2135982/1
component: BBa_K628000

https://synbiohub.org/public/igem/BBa_K628006/annotation2135982/range2135982/1
type: Range
persistent identity: range2135982
display id: range2135982
version: 1
direction: direction/reverse
top level: BBa_K628006
start: 157
end: 216
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2135989
display id: annotation2135989
version: 1
top level: BBa_K628006
location: https://synbiohub.org/public/igem/BBa_K628006/annotation2135989/range2135989/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K628006/annotation2135989/range2135989/1
type: Range
persistent identity: range2135989
display id: range2135989
version: 1
direction: direction/reverse
top level: BBa_K628006
start: 225
end: 353
orientation: inline

BBa_J61101
type: SequenceAnnotation
persistent identity: annotation2135981
display id: annotation2135981
version: 1
top level: BBa_K628006
location: https://synbiohub.org/public/igem/BBa_K628006/annotation2135981/range2135981/1
component: BBa_J61101

https://synbiohub.org/public/igem/BBa_K628006/annotation2135981/range2135981/1
type: Range
persistent identity: range2135981
display id: range2135981
version: 1
direction: direction/reverse
top level: BBa_K628006
start: 139
end: 150
orientation: inline

BBa_K206000
type: SequenceAnnotation
persistent identity: annotation2135980
display id: annotation2135980
version: 1
top level: BBa_K628006
location: https://synbiohub.org/public/igem/BBa_K628006/annotation2135980/range2135980/1
component: BBa_K206000

https://synbiohub.org/public/igem/BBa_K628006/annotation2135980/range2135980/1
type: Range
persistent identity: range2135980
display id: range2135980
version: 1
direction: direction/reverse
top level: BBa_K628006
start: 1
end: 130
orientation: inline

BBa_K660200
type: ComponentDefinition
persistent identity: BBa K660200
display id: BBa_K660200
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Latherin (Surfactant Protein)
creator: Glasgow Uni 2011
created: 2011-09-15T11:00:00Z
modified: 2016-02-03T10:51:32Z
discontinued: false
dominant: false
experience: experience/None
group u list: _837_
m user id: 4206
owner id: 9421
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Latherin is a surfactant protein. Its normal biological function is temperature regulation and is believed to function by enhancing evaporation from the pelt. Due to its ability to bind to hydrophobic surfaces, the protein is being investigated for its possible ability to aid in breaking up biofilms. Caution - suspected allergen
mutable notes: Illegal restriction sites - site directed mutagenesis
mutable provenance: Originally isolated from horse sweat. Received from researchers
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K660200_sequence/1