//direction/bidirectional
type: Collection
persistent identity: direction bidirectional
display id: direction_bidirectional
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
owned by: james
owned by: myers
member: BBa_J31013
member: BBa_K113011
member: BBa_K180004
member: BBa_B1007
member: BBa_B0011
member: BBa_K113010
member: BBa_B0050
member: BBa_B1001
member: revPlam
member: BBa_I714889
member: BBa_B0024
member: BBa_K180012
member: BBa_K113012
member: BBa_K259006
member: BBa_B1008
member: BBa_K187025
member: BBa_K180017
member: BBa_K598022
member: BBa_J44002
member: BBa_B1009
member: PN
member: BBa_K180010
member: BBa_B0014
member: GLF
member: BBa_K180014
member: BBa_B0021
member: P1
member: BBa_T1004
member: BBa_K598002
member: BBa_K180006
member: FlipFlop
member: P3
member: BBa_K180019
member: BBa_B0051
member: BBa_K180011
member: BBa_K180015

BBa_K180017
type: ComponentDefinition
persistent identity: BBa K180017
display id: BBa_K180017
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Rock-paper-scissors - Scissors primary plasmid
creator: Richard Wilson, Stephanie Chambers
created: 2009-10-20T11:00:00Z
modified: 2015-05-08T01:11:06Z
discontinued: false
dominant: false
experience: experience/None
group u list: _278_
m user id: 0
owner id: 4082
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: Composite part, consisting of BBa_K180018 and BBa_K180019, but with BBa_K180018 reading along the reverse strand, and BBa_K180019 reading along the forward strand. Forms the 'scissors' primary plasmid in Team:Southampton's rock-paper-scissors project design.
mutable notes: BBa_K180018 reading along reverse strand, and BBa_K180019 reading along the forward strand to ensure the constitutive promoter BBa_J23119 (present in BBa_K180018) has no effect over mBanana or LuxI expression in BBa_K180019.
mutable provenance: See relevant biobrick component parts (BBa_K180018 and BBa_K180019).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: mBanana
sequence annotation: B0034
sequence annotation: -35
sequence annotation: C0179
sequence annotation: K180000
sequence annotation: K180018
sequence annotation: K180019
sequence annotation: lacIq
sequence annotation: C0161
sequence annotation: Ptac
sequence annotation: conserved
sequence annotation: B0015
sequence annotation: lasR
sequence annotation: lacI
sequence annotation: -10
sequence annotation: J23119
sequence annotation: K180009
sequence: https://synbiohub.org/public/igem/BBa_K180017_sequence/1

mBanana
type: SequenceAnnotation
persistent identity: annotation2058470
display id: annotation2058470
version: 1
top level: BBa_K180017
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K180017/annotation2058470/range2058470/1

https://synbiohub.org/public/igem/BBa_K180017/annotation2058470/range2058470/1
type: Range
persistent identity: range2058470
display id: range2058470
version: 1
direction: direction/forward
top level: BBa_K180017
start: 2403
end: 3110
orientation: inline

B0034
type: SequenceAnnotation
persistent identity: annotation2058471
display id: annotation2058471
version: 1
top level: BBa_K180017
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K180017/annotation2058471/range2058471/1

https://synbiohub.org/public/igem/BBa_K180017/annotation2058471/range2058471/1
type: Range
persistent identity: range2058471
display id: range2058471
version: 1
direction: direction/forward
top level: BBa_K180017
start: 3119
end: 3130
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2058461
display id: annotation2058461
version: 1
top level: BBa_K180017
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K180017/annotation2058461/range2058461/1

https://synbiohub.org/public/igem/BBa_K180017/annotation2058461/range2058461/1
type: Range
persistent identity: range2058461
display id: range2058461
version: 1
direction: direction/reverse
top level: BBa_K180017
start: 916
end: 921
orientation: inline

C0179
type: SequenceAnnotation
persistent identity: annotation2058456
display id: annotation2058456
version: 1
top level: BBa_K180017
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K180017/annotation2058456/range2058456/1

https://synbiohub.org/public/igem/BBa_K180017/annotation2058456/range2058456/1
type: Range
persistent identity: range2058456
display id: range2058456
version: 1
direction: direction/reverse
top level: BBa_K180017
start: 138
end: 860
orientation: inline

K180000
type: SequenceAnnotation
persistent identity: annotation2058463
display id: annotation2058463
version: 1
top level: BBa_K180017
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K180017/annotation2058463/range2058463/1

https://synbiohub.org/public/igem/BBa_K180017/annotation2058463/range2058463/1
type: Range
persistent identity: range2058463
display id: range2058463
version: 1
direction: direction/forward
top level: BBa_K180017
start: 930
end: 2376
orientation: inline

K180018
type: SequenceAnnotation
persistent identity: annotation2058454
display id: annotation2058454
version: 1
top level: BBa_K180017
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K180017/annotation2058454/range2058454/1

https://synbiohub.org/public/igem/BBa_K180017/annotation2058454/range2058454/1
type: Range
persistent identity: range2058454
display id: range2058454
version: 1
direction: direction/reverse
top level: BBa_K180017
start: 1
end: 921
orientation: inline

K180019
type: SequenceAnnotation
persistent identity: annotation2058464
display id: annotation2058464
version: 1
top level: BBa_K180017
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K180017/annotation2058464/range2058464/1

https://synbiohub.org/public/igem/BBa_K180017/annotation2058464/range2058464/1
type: Range
persistent identity: range2058464
display id: range2058464
version: 1
direction: direction/forward
top level: BBa_K180017
start: 930
end: 3858
orientation: inline

lacIq
type: SequenceAnnotation
persistent identity: annotation2058462
display id: annotation2058462
version: 1
top level: BBa_K180017
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K180017/annotation2058462/range2058462/1

https://synbiohub.org/public/igem/BBa_K180017/annotation2058462/range2058462/1
type: Range
persistent identity: range2058462
display id: range2058462
version: 1
direction: direction/forward
top level: BBa_K180017
start: 930
end: 2011
orientation: inline

C0161
type: SequenceAnnotation
persistent identity: annotation2058472
display id: annotation2058472
version: 1
top level: BBa_K180017
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K180017/annotation2058472/range2058472/1

https://synbiohub.org/public/igem/BBa_K180017/annotation2058472/range2058472/1
type: Range
persistent identity: range2058472
display id: range2058472
version: 1
direction: direction/forward
top level: BBa_K180017
start: 3137
end: 3721
orientation: inline

Ptac
type: SequenceAnnotation
persistent identity: annotation2058465
display id: annotation2058465
version: 1
top level: BBa_K180017
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K180017/annotation2058465/range2058465/1

https://synbiohub.org/public/igem/BBa_K180017/annotation2058465/range2058465/1
type: Range
persistent identity: range2058465
display id: range2058465
version: 1
direction: direction/forward
top level: BBa_K180017
start: 2254
end: 2281
orientation: inline

B0034
type: SequenceAnnotation
persistent identity: annotation2058457
display id: annotation2058457
version: 1
top level: BBa_K180017
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K180017/annotation2058457/range2058457/1

https://synbiohub.org/public/igem/BBa_K180017/annotation2058457/range2058457/1
type: Range
persistent identity: range2058457
display id: range2058457
version: 1
direction: direction/reverse
top level: BBa_K180017
start: 867
end: 878
orientation: inline

conserved
type: SequenceAnnotation
persistent identity: annotation2058467
display id: annotation2058467
version: 1
top level: BBa_K180017
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K180017/annotation2058467/range2058467/1

https://synbiohub.org/public/igem/BBa_K180017/annotation2058467/range2058467/1
type: Range
persistent identity: range2058467
display id: range2058467
version: 1
direction: direction/forward
top level: BBa_K180017
start: 2389
end: 2392
orientation: inline

B0015
type: SequenceAnnotation
persistent identity: annotation2058453
display id: annotation2058453
version: 1
top level: BBa_K180017
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K180017/annotation2058453/range2058453/1

https://synbiohub.org/public/igem/BBa_K180017/annotation2058453/range2058453/1
type: Range
persistent identity: range2058453
display id: range2058453
version: 1
direction: direction/reverse
top level: BBa_K180017
start: 1
end: 129
orientation: inline

lasR
type: SequenceAnnotation
persistent identity: annotation2058455
display id: annotation2058455
version: 1
top level: BBa_K180017
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K180017/annotation2058455/range2058455/1

https://synbiohub.org/public/igem/BBa_K180017/annotation2058455/range2058455/1
type: Range
persistent identity: range2058455
display id: range2058455
version: 1
direction: direction/reverse
top level: BBa_K180017
start: 138
end: 860
orientation: inline

conserved
type: SequenceAnnotation
persistent identity: annotation2058458
display id: annotation2058458
version: 1
top level: BBa_K180017
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K180017/annotation2058458/range2058458/1

https://synbiohub.org/public/igem/BBa_K180017/annotation2058458/range2058458/1
type: Range
persistent identity: range2058458
display id: range2058458
version: 1
direction: direction/reverse
top level: BBa_K180017
start: 871
end: 874
orientation: inline

conserved
type: SequenceAnnotation
persistent identity: annotation2058468
display id: annotation2058468
version: 1
top level: BBa_K180017
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K180017/annotation2058468/range2058468/1

https://synbiohub.org/public/igem/BBa_K180017/annotation2058468/range2058468/1
type: Range
persistent identity: range2058468
display id: range2058468
version: 1
direction: direction/forward
top level: BBa_K180017
start: 2390
end: 2393
orientation: inline

lacI
type: SequenceAnnotation
persistent identity: annotation2058473
display id: annotation2058473
version: 1
top level: BBa_K180017
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K180017/annotation2058473/range2058473/1

https://synbiohub.org/public/igem/BBa_K180017/annotation2058473/range2058473/1
type: Range
persistent identity: range2058473
display id: range2058473
version: 1
direction: direction/forward
top level: BBa_K180017
start: 3137
end: 3721
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2058460
display id: annotation2058460
version: 1
top level: BBa_K180017
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K180017/annotation2058460/range2058460/1

https://synbiohub.org/public/igem/BBa_K180017/annotation2058460/range2058460/1
type: Range
persistent identity: range2058460
display id: range2058460
version: 1
direction: direction/reverse
top level: BBa_K180017
start: 893
end: 898
orientation: inline

J23119
type: SequenceAnnotation
persistent identity: annotation2058459
display id: annotation2058459
version: 1
top level: BBa_K180017
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K180017/annotation2058459/range2058459/1

https://synbiohub.org/public/igem/BBa_K180017/annotation2058459/range2058459/1
type: Range
persistent identity: range2058459
display id: range2058459
version: 1
direction: direction/reverse
top level: BBa_K180017
start: 887
end: 921
orientation: inline

K180009
type: SequenceAnnotation
persistent identity: annotation2058469
display id: annotation2058469
version: 1
top level: BBa_K180017
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K180017/annotation2058469/range2058469/1

https://synbiohub.org/public/igem/BBa_K180017/annotation2058469/range2058469/1
type: Range
persistent identity: range2058469
display id: range2058469
version: 1
direction: direction/forward
top level: BBa_K180017
start: 2403
end: 3110
orientation: inline

B0015
type: SequenceAnnotation
persistent identity: annotation2058474
display id: annotation2058474
version: 1
top level: BBa_K180017
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K180017/annotation2058474/range2058474/1

https://synbiohub.org/public/igem/BBa_K180017/annotation2058474/range2058474/1
type: Range
persistent identity: range2058474
display id: range2058474
version: 1
direction: direction/forward
top level: BBa_K180017
start: 3730
end: 3858
orientation: inline

B0034
type: SequenceAnnotation
persistent identity: annotation2058466
display id: annotation2058466
version: 1
top level: BBa_K180017
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K180017/annotation2058466/range2058466/1

https://synbiohub.org/public/igem/BBa_K180017/annotation2058466/range2058466/1
type: Range
persistent identity: range2058466
display id: range2058466
version: 1
direction: direction/forward
top level: BBa_K180017
start: 2385
end: 2396
orientation: inline

BBa_K180004
type: ComponentDefinition
persistent identity: BBa K180004
display id: BBa_K180004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Game of Life - Primary plasmid
creator: Richard Wilson, Stephanie Chambers
created: 2009-10-15T11:00:00Z
modified: 2015-05-08T01:11:06Z
discontinued: false
dominant: false
experience: experience/None
group u list: _278_
m user id: 0
owner id: 4082
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: Composite part, consisting of BBa_K180005 and BBa_K180006, but with BBa_K180005 reading along reverse strand, and BBa_K180006 reading along the forward strand.
mutable notes: BBa_K180005 reading along reverse strand, and BBa_K180006 reading along the forward strand to ensure the constitutive promoter BBa_J23119 (present in BBa_K180005) has no effect over LacI or GFP-LVA expression in BBa_K180006.
mutable provenance: See relevant biobrick component parts (BBa_K180005 and BBa_K180006)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: B0015
sequence annotation: LVA
sequence annotation: conserved
sequence annotation: B0034
sequence annotation: K180001
sequence annotation: -35
sequence annotation: K180006
sequence annotation: R0062
sequence annotation: start
sequence annotation: lacI
sequence annotation: -10
sequence annotation: LuxR/HSL
sequence annotation: C0012
sequence annotation: K180005
sequence annotation: luxR
sequence annotation: J23119
sequence annotation: GFPmut3b
sequence annotation: C0062
sequence: https://synbiohub.org/public/igem/BBa_K180004_sequence/1

B0015
type: SequenceAnnotation
persistent identity: annotation2058545
display id: annotation2058545
version: 1
top level: BBa_K180004
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K180004/annotation2058545/range2058545/1

https://synbiohub.org/public/igem/BBa_K180004/annotation2058545/range2058545/1
type: Range
persistent identity: range2058545
display id: range2058545
version: 1
direction: direction/forward
top level: BBa_K180004
start: 3010
end: 3138
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation2058539
display id: annotation2058539
version: 1
top level: BBa_K180004
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K180004/annotation2058539/range2058539/1

https://synbiohub.org/public/igem/BBa_K180004/annotation2058539/range2058539/1
type: Range
persistent identity: range2058539
display id: range2058539
version: 1
direction: direction/forward
top level: BBa_K180004
start: 2158
end: 2196
orientation: inline

conserved
type: SequenceAnnotation
persistent identity: annotation2058538
display id: annotation2058538
version: 1
top level: BBa_K180004
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K180004/annotation2058538/range2058538/1

https://synbiohub.org/public/igem/BBa_K180004/annotation2058538/range2058538/1
type: Range
persistent identity: range2058538
display id: range2058538
version: 1
direction: direction/forward
top level: BBa_K180004
start: 1055
end: 1058
orientation: inline

B0034
type: SequenceAnnotation
persistent identity: annotation2058542
display id: annotation2058542
version: 1
top level: BBa_K180004
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K180004/annotation2058542/range2058542/1

https://synbiohub.org/public/igem/BBa_K180004/annotation2058542/range2058542/1
type: Range
persistent identity: range2058542
display id: range2058542
version: 1
direction: direction/forward
top level: BBa_K180004
start: 2230
end: 2241
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation2058544
display id: annotation2058544
version: 1
top level: BBa_K180004
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K180004/annotation2058544/range2058544/1

https://synbiohub.org/public/igem/BBa_K180004/annotation2058544/range2058544/1
type: Range
persistent identity: range2058544
display id: range2058544
version: 1
direction: direction/forward
top level: BBa_K180004
start: 2968
end: 3001
orientation: inline

K180001
type: SequenceAnnotation
persistent identity: annotation2058541
display id: annotation2058541
version: 1
top level: BBa_K180004
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K180004/annotation2058541/range2058541/1

https://synbiohub.org/public/igem/BBa_K180004/annotation2058541/range2058541/1
type: Range
persistent identity: range2058541
display id: range2058541
version: 1
direction: direction/forward
top level: BBa_K180004
start: 2248
end: 3001
orientation: inline

B0034
type: SequenceAnnotation
persistent identity: annotation2058525
display id: annotation2058525
version: 1
top level: BBa_K180004
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K180004/annotation2058525/range2058525/1

https://synbiohub.org/public/igem/BBa_K180004/annotation2058525/range2058525/1
type: Range
persistent identity: range2058525
display id: range2058525
version: 1
direction: direction/reverse
top level: BBa_K180004
start: 925
end: 936
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2058546
display id: annotation2058546
version: 1
top level: BBa_K180004
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K180004/annotation2058546/range2058546/1

https://synbiohub.org/public/igem/BBa_K180004/annotation2058546/range2058546/1
type: Range
persistent identity: range2058546
display id: range2058546
version: 1
direction: direction/forward
top level: BBa_K180004
start: 1007
end: 1012
orientation: inline

conserved
type: SequenceAnnotation
persistent identity: annotation2058543
display id: annotation2058543
version: 1
top level: BBa_K180004
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K180004/annotation2058543/range2058543/1

https://synbiohub.org/public/igem/BBa_K180004/annotation2058543/range2058543/1
type: Range
persistent identity: range2058543
display id: range2058543
version: 1
direction: direction/forward
top level: BBa_K180004
start: 2234
end: 2237
orientation: inline

K180006
type: SequenceAnnotation
persistent identity: annotation2058532
display id: annotation2058532
version: 1
top level: BBa_K180004
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K180004/annotation2058532/range2058532/1

https://synbiohub.org/public/igem/BBa_K180004/annotation2058532/range2058532/1
type: Range
persistent identity: range2058532
display id: range2058532
version: 1
direction: direction/forward
top level: BBa_K180004
start: 988
end: 3138
orientation: inline

R0062
type: SequenceAnnotation
persistent identity: annotation2058531
display id: annotation2058531
version: 1
top level: BBa_K180004
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K180004/annotation2058531/range2058531/1

https://synbiohub.org/public/igem/BBa_K180004/annotation2058531/range2058531/1
type: Range
persistent identity: range2058531
display id: range2058531
version: 1
direction: direction/forward
top level: BBa_K180004
start: 988
end: 1042
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2058534
display id: annotation2058534
version: 1
top level: BBa_K180004
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K180004/annotation2058534/range2058534/1

https://synbiohub.org/public/igem/BBa_K180004/annotation2058534/range2058534/1
type: Range
persistent identity: range2058534
display id: range2058534
version: 1
direction: direction/forward
top level: BBa_K180004
start: 1040
end: 1040
orientation: inline

lacI
type: SequenceAnnotation
persistent identity: annotation2058536
display id: annotation2058536
version: 1
top level: BBa_K180004
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K180004/annotation2058536/range2058536/1

https://synbiohub.org/public/igem/BBa_K180004/annotation2058536/range2058536/1
type: Range
persistent identity: range2058536
display id: range2058536
version: 1
direction: direction/forward
top level: BBa_K180004
start: 1069
end: 2157
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2058528
display id: annotation2058528
version: 1
top level: BBa_K180004
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K180004/annotation2058528/range2058528/1

https://synbiohub.org/public/igem/BBa_K180004/annotation2058528/range2058528/1
type: Range
persistent identity: range2058528
display id: range2058528
version: 1
direction: direction/reverse
top level: BBa_K180004
start: 951
end: 956
orientation: inline

B0034
type: SequenceAnnotation
persistent identity: annotation2058537
display id: annotation2058537
version: 1
top level: BBa_K180004
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K180004/annotation2058537/range2058537/1

https://synbiohub.org/public/igem/BBa_K180004/annotation2058537/range2058537/1
type: Range
persistent identity: range2058537
display id: range2058537
version: 1
direction: direction/forward
top level: BBa_K180004
start: 1051
end: 1062
orientation: inline

LuxR/HSL
type: SequenceAnnotation
persistent identity: annotation2058529
display id: annotation2058529
version: 1
top level: BBa_K180004
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K180004/annotation2058529/range2058529/1

https://synbiohub.org/public/igem/BBa_K180004/annotation2058529/range2058529/1
type: Range
persistent identity: range2058529
display id: range2058529
version: 1
direction: direction/forward
top level: BBa_K180004
start: 988
end: 1007
orientation: inline

C0012
type: SequenceAnnotation
persistent identity: annotation2058535
display id: annotation2058535
version: 1
top level: BBa_K180004
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K180004/annotation2058535/range2058535/1

https://synbiohub.org/public/igem/BBa_K180004/annotation2058535/range2058535/1
type: Range
persistent identity: range2058535
display id: range2058535
version: 1
direction: direction/forward
top level: BBa_K180004
start: 1069
end: 2196
orientation: inline

K180005
type: SequenceAnnotation
persistent identity: annotation2058521
display id: annotation2058521
version: 1
top level: BBa_K180004
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K180004/annotation2058521/range2058521/1

https://synbiohub.org/public/igem/BBa_K180004/annotation2058521/range2058521/1
type: Range
persistent identity: range2058521
display id: range2058521
version: 1
direction: direction/reverse
top level: BBa_K180004
start: 1
end: 979
orientation: inline

luxR
type: SequenceAnnotation
persistent identity: annotation2058524
display id: annotation2058524
version: 1
top level: BBa_K180004
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K180004/annotation2058524/range2058524/1

https://synbiohub.org/public/igem/BBa_K180004/annotation2058524/range2058524/1
type: Range
persistent identity: range2058524
display id: range2058524
version: 1
direction: direction/reverse
top level: BBa_K180004
start: 163
end: 912
orientation: inline

J23119
type: SequenceAnnotation
persistent identity: annotation2058527
display id: annotation2058527
version: 1
top level: BBa_K180004
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K180004/annotation2058527/range2058527/1

https://synbiohub.org/public/igem/BBa_K180004/annotation2058527/range2058527/1
type: Range
persistent identity: range2058527
display id: range2058527
version: 1
direction: direction/reverse
top level: BBa_K180004
start: 945
end: 979
orientation: inline

GFPmut3b
type: SequenceAnnotation
persistent identity: annotation2058540
display id: annotation2058540
version: 1
top level: BBa_K180004
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K180004/annotation2058540/range2058540/1

https://synbiohub.org/public/igem/BBa_K180004/annotation2058540/range2058540/1
type: Range
persistent identity: range2058540
display id: range2058540
version: 1
direction: direction/forward
top level: BBa_K180004
start: 2248
end: 2967
orientation: inline

C0062
type: SequenceAnnotation
persistent identity: annotation2058523
display id: annotation2058523
version: 1
top level: BBa_K180004
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K180004/annotation2058523/range2058523/1

https://synbiohub.org/public/igem/BBa_K180004/annotation2058523/range2058523/1
type: Range
persistent identity: range2058523
display id: range2058523
version: 1
direction: direction/reverse
top level: BBa_K180004
start: 163
end: 918
orientation: inline

B0015
type: SequenceAnnotation
persistent identity: annotation2058522
display id: annotation2058522
version: 1
top level: BBa_K180004
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K180004/annotation2058522/range2058522/1

https://synbiohub.org/public/igem/BBa_K180004/annotation2058522/range2058522/1
type: Range
persistent identity: range2058522
display id: range2058522
version: 1
direction: direction/reverse
top level: BBa_K180004
start: 1
end: 129
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2058530
display id: annotation2058530
version: 1
top level: BBa_K180004
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K180004/annotation2058530/range2058530/1

https://synbiohub.org/public/igem/BBa_K180004/annotation2058530/range2058530/1
type: Range
persistent identity: range2058530
display id: range2058530
version: 1
direction: direction/reverse
top level: BBa_K180004
start: 974
end: 979
orientation: inline

conserved
type: SequenceAnnotation
persistent identity: annotation2058526
display id: annotation2058526
version: 1
top level: BBa_K180004
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K180004/annotation2058526/range2058526/1

https://synbiohub.org/public/igem/BBa_K180004/annotation2058526/range2058526/1
type: Range
persistent identity: range2058526
display id: range2058526
version: 1
direction: direction/reverse
top level: BBa_K180004
start: 929
end: 932
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2058533
display id: annotation2058533
version: 1
top level: BBa_K180004
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K180004/annotation2058533/range2058533/1

https://synbiohub.org/public/igem/BBa_K180004/annotation2058533/range2058533/1
type: Range
persistent identity: range2058533
display id: range2058533
version: 1
direction: direction/forward
top level: BBa_K180004
start: 1029
end: 1034
orientation: inline

BBa_K187025
type: ComponentDefinition
persistent identity: BBa K187025
display id: BBa_K187025
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: terminator in pAB, BioBytes plasmid
creator: Julia Pon
created: 2009-10-17T11:00:00Z
modified: 2015-05-08T01:11:07Z
discontinued: false
dominant: true
experience: experience/None
group u list: _286_
m user id: 0
owner id: 4048
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part contains a bidirectional, high efficiency terminator, in the BioBytes universal plasmid pAB.See the BioBytes RFC for details on the BioBytes assembly method.
mutable notes: A terminator with long sticky ends can be prepared from this plasmid and assembled downstream of a gene using the Biobytes method. The addition of one DNA segment with the BioBytes method takes only 20 minutes. This ability to easily 'mix and match' terminators and genes allows rapid testing of different terminator designs.
mutable provenance: The terminator is derived from part b1006. pAB is entered as part K187000.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: XbaI
sequence annotation: PstI
sequence: https://synbiohub.org/public/igem/BBa_K187025_sequence/1

XbaI
type: SequenceAnnotation
persistent identity: annotation2051724
display id: annotation2051724
version: 1
top level: BBa_K187025
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K187025/annotation2051724/range2051724/1

https://synbiohub.org/public/igem/BBa_K187025/annotation2051724/range2051724/1
type: Range
persistent identity: range2051724
display id: range2051724
version: 1
direction: direction/forward
top level: BBa_K187025
start: 10
end: 15
orientation: inline

PstI
type: SequenceAnnotation
persistent identity: annotation2051725
display id: annotation2051725
version: 1
top level: BBa_K187025
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K187025/annotation2051725/range2051725/1

https://synbiohub.org/public/igem/BBa_K187025/annotation2051725/range2051725/1
type: Range
persistent identity: range2051725
display id: range2051725
version: 1
direction: direction/forward
top level: BBa_K187025
start: 53
end: 58
orientation: inline

BBa_B0011
type: ComponentDefinition
persistent identity: BBa B0011
display id: BBa_B0011
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LuxICDABEG (+/-)
creator: Reshma Shetty
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Bidirectional transcriptional terminator consisting of a 22 bp stem-loop.
mutable notes: In the naturally-occuring sequence there is a mismatch in the stem of the stem loop. This can be corrected via an A->G mutation (at position 40 -- sequence coordinate/not MFOLD coordinate). The above sequence does not reflect this mutation (but the MFOLD image does). This terminator's location cannot be found using some inverted repeat detectors like PALINDROME because it is too short and contains a mismatch. This one was found with the help of Tom Knight. It lies between two coding regions that point towards eachother.
mutable provenance: Derived from luxICDABEG operon terminator of Vibrio fischeri <genbank>AF170104</genbank>.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0011
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0011_sequence/1

BBa_B0011
type: SequenceAnnotation
persistent identity: annotation7019
display id: annotation7019
version: 1
top level: BBa_B0011
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_B0011/annotation7019/range7019/1

https://synbiohub.org/public/igem/BBa_B0011/annotation7019/range7019/1
type: Range
persistent identity: range7019
display id: range7019
version: 1
direction: direction/forward
top level: BBa_B0011
start: 1
end: 46
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation1683
display id: annotation1683
version: 1
top level: BBa_B0011
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_B0011/annotation1683/range1683/1

https://synbiohub.org/public/igem/BBa_B0011/annotation1683/range1683/1
type: Range
persistent identity: range1683
display id: range1683
version: 1
direction: direction/forward
top level: BBa_B0011
start: 13
end: 35
orientation: inline

BBa_K180014
type: ComponentDefinition
persistent identity: BBa K180014
display id: BBa_K180014
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Rock-paper-scissors - Paper primary plasmid
creator: Richard Wilson, Stephanie Chambers
created: 2009-10-20T11:00:00Z
modified: 2015-05-08T01:11:06Z
discontinued: false
dominant: false
experience: experience/None
group u list: _278_
m user id: 0
owner id: 4082
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: Composite part, consisting of BBa_K180005 and BBa_K180015, but with BBa_K180005 reading along the reverse strand, and BBa_K180015 reading along the forward strand. Forms the 'paper' primary plasmid in Team:Southampton's rock-paper-scissors project design.
mutable notes: BBa_K180005 reading along reverse strand, and BBa_K180015 reading along the forward strand to ensure the constitutive promoter BBa_J23119 (present in BBa_K180005) has no effect over GFP or RhlI expression in BBa_K180015.
mutable provenance: See relevant biobrick component parts (BBa_K180011 and BBa_K180012).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: lacIq
sequence annotation: B0034
sequence annotation: J23119
sequence annotation: Ptac
sequence annotation: K180000
sequence annotation: -10
sequence annotation: B0015
sequence annotation: -35
sequence annotation: conserved
sequence annotation: luxR
sequence annotation: GFPmut3b
sequence annotation: K180005
sequence annotation: E0040
sequence annotation: rhlI
sequence annotation: C0062
sequence annotation: K180015
sequence annotation: C0170
sequence: https://synbiohub.org/public/igem/BBa_K180014_sequence/1

lacIq
type: SequenceAnnotation
persistent identity: annotation2058337
display id: annotation2058337
version: 1
top level: BBa_K180014
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K180014/annotation2058337/range2058337/1

https://synbiohub.org/public/igem/BBa_K180014/annotation2058337/range2058337/1
type: Range
persistent identity: range2058337
display id: range2058337
version: 1
direction: direction/forward
top level: BBa_K180014
start: 988
end: 2069
orientation: inline

B0034
type: SequenceAnnotation
persistent identity: annotation2058331
display id: annotation2058331
version: 1
top level: BBa_K180014
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K180014/annotation2058331/range2058331/1

https://synbiohub.org/public/igem/BBa_K180014/annotation2058331/range2058331/1
type: Range
persistent identity: range2058331
display id: range2058331
version: 1
direction: direction/reverse
top level: BBa_K180014
start: 925
end: 936
orientation: inline

J23119
type: SequenceAnnotation
persistent identity: annotation2058333
display id: annotation2058333
version: 1
top level: BBa_K180014
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K180014/annotation2058333/range2058333/1

https://synbiohub.org/public/igem/BBa_K180014/annotation2058333/range2058333/1
type: Range
persistent identity: range2058333
display id: range2058333
version: 1
direction: direction/reverse
top level: BBa_K180014
start: 945
end: 979
orientation: inline

Ptac
type: SequenceAnnotation
persistent identity: annotation2058338
display id: annotation2058338
version: 1
top level: BBa_K180014
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K180014/annotation2058338/range2058338/1

https://synbiohub.org/public/igem/BBa_K180014/annotation2058338/range2058338/1
type: Range
persistent identity: range2058338
display id: range2058338
version: 1
direction: direction/forward
top level: BBa_K180014
start: 2312
end: 2339
orientation: inline

K180000
type: SequenceAnnotation
persistent identity: annotation2058336
display id: annotation2058336
version: 1
top level: BBa_K180014
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K180014/annotation2058336/range2058336/1

https://synbiohub.org/public/igem/BBa_K180014/annotation2058336/range2058336/1
type: Range
persistent identity: range2058336
display id: range2058336
version: 1
direction: direction/forward
top level: BBa_K180014
start: 988
end: 2434
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2058335
display id: annotation2058335
version: 1
top level: BBa_K180014
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K180014/annotation2058335/range2058335/1

https://synbiohub.org/public/igem/BBa_K180014/annotation2058335/range2058335/1
type: Range
persistent identity: range2058335
display id: range2058335
version: 1
direction: direction/reverse
top level: BBa_K180014
start: 951
end: 956
orientation: inline

B0015
type: SequenceAnnotation
persistent identity: annotation2058324
display id: annotation2058324
version: 1
top level: BBa_K180014
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K180014/annotation2058324/range2058324/1

https://synbiohub.org/public/igem/BBa_K180014/annotation2058324/range2058324/1
type: Range
persistent identity: range2058324
display id: range2058324
version: 1
direction: direction/reverse
top level: BBa_K180014
start: 1
end: 129
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2058334
display id: annotation2058334
version: 1
top level: BBa_K180014
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K180014/annotation2058334/range2058334/1

https://synbiohub.org/public/igem/BBa_K180014/annotation2058334/range2058334/1
type: Range
persistent identity: range2058334
display id: range2058334
version: 1
direction: direction/reverse
top level: BBa_K180014
start: 975
end: 979
orientation: inline

conserved
type: SequenceAnnotation
persistent identity: annotation2058332
display id: annotation2058332
version: 1
top level: BBa_K180014
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K180014/annotation2058332/range2058332/1

https://synbiohub.org/public/igem/BBa_K180014/annotation2058332/range2058332/1
type: Range
persistent identity: range2058332
display id: range2058332
version: 1
direction: direction/reverse
top level: BBa_K180014
start: 929
end: 932
orientation: inline

luxR
type: SequenceAnnotation
persistent identity: annotation2058326
display id: annotation2058326
version: 1
top level: BBa_K180014
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K180014/annotation2058326/range2058326/1

https://synbiohub.org/public/igem/BBa_K180014/annotation2058326/range2058326/1
type: Range
persistent identity: range2058326
display id: range2058326
version: 1
direction: direction/reverse
top level: BBa_K180014
start: 169
end: 918
orientation: inline

GFPmut3b
type: SequenceAnnotation
persistent identity: annotation2058340
display id: annotation2058340
version: 1
top level: BBa_K180014
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K180014/annotation2058340/range2058340/1

https://synbiohub.org/public/igem/BBa_K180014/annotation2058340/range2058340/1
type: Range
persistent identity: range2058340
display id: range2058340
version: 1
direction: direction/forward
top level: BBa_K180014
start: 2461
end: 3180
orientation: inline

B0034
type: SequenceAnnotation
persistent identity: annotation2058327
display id: annotation2058327
version: 1
top level: BBa_K180014
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K180014/annotation2058327/range2058327/1

https://synbiohub.org/public/igem/BBa_K180014/annotation2058327/range2058327/1
type: Range
persistent identity: range2058327
display id: range2058327
version: 1
direction: direction/forward
top level: BBa_K180014
start: 2443
end: 2454
orientation: inline

conserved
type: SequenceAnnotation
persistent identity: annotation2058328
display id: annotation2058328
version: 1
top level: BBa_K180014
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K180014/annotation2058328/range2058328/1

https://synbiohub.org/public/igem/BBa_K180014/annotation2058328/range2058328/1
type: Range
persistent identity: range2058328
display id: range2058328
version: 1
direction: direction/forward
top level: BBa_K180014
start: 2447
end: 2450
orientation: inline

K180005
type: SequenceAnnotation
persistent identity: annotation2058320
display id: annotation2058320
version: 1
top level: BBa_K180014
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K180014/annotation2058320/range2058320/1

https://synbiohub.org/public/igem/BBa_K180014/annotation2058320/range2058320/1
type: Range
persistent identity: range2058320
display id: range2058320
version: 1
direction: direction/reverse
top level: BBa_K180014
start: 1
end: 979
orientation: inline

E0040
type: SequenceAnnotation
persistent identity: annotation2058339
display id: annotation2058339
version: 1
top level: BBa_K180014
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K180014/annotation2058339/range2058339/1

https://synbiohub.org/public/igem/BBa_K180014/annotation2058339/range2058339/1
type: Range
persistent identity: range2058339
display id: range2058339
version: 1
direction: direction/forward
top level: BBa_K180014
start: 2461
end: 3180
orientation: inline

B0015
type: SequenceAnnotation
persistent identity: annotation2058343
display id: annotation2058343
version: 1
top level: BBa_K180014
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K180014/annotation2058343/range2058343/1

https://synbiohub.org/public/igem/BBa_K180014/annotation2058343/range2058343/1
type: Range
persistent identity: range2058343
display id: range2058343
version: 1
direction: direction/forward
top level: BBa_K180014
start: 3824
end: 3952
orientation: inline

rhlI
type: SequenceAnnotation
persistent identity: annotation2058342
display id: annotation2058342
version: 1
top level: BBa_K180014
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K180014/annotation2058342/range2058342/1

https://synbiohub.org/public/igem/BBa_K180014/annotation2058342/range2058342/1
type: Range
persistent identity: range2058342
display id: range2058342
version: 1
direction: direction/forward
top level: BBa_K180014
start: 3207
end: 3815
orientation: inline

conserved
type: SequenceAnnotation
persistent identity: annotation2058330
display id: annotation2058330
version: 1
top level: BBa_K180014
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K180014/annotation2058330/range2058330/1

https://synbiohub.org/public/igem/BBa_K180014/annotation2058330/range2058330/1
type: Range
persistent identity: range2058330
display id: range2058330
version: 1
direction: direction/forward
top level: BBa_K180014
start: 3193
end: 3196
orientation: inline

C0062
type: SequenceAnnotation
persistent identity: annotation2058325
display id: annotation2058325
version: 1
top level: BBa_K180014
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K180014/annotation2058325/range2058325/1

https://synbiohub.org/public/igem/BBa_K180014/annotation2058325/range2058325/1
type: Range
persistent identity: range2058325
display id: range2058325
version: 1
direction: direction/reverse
top level: BBa_K180014
start: 163
end: 918
orientation: inline

K180015
type: SequenceAnnotation
persistent identity: annotation2058321
display id: annotation2058321
version: 1
top level: BBa_K180014
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K180014/annotation2058321/range2058321/1

https://synbiohub.org/public/igem/BBa_K180014/annotation2058321/range2058321/1
type: Range
persistent identity: range2058321
display id: range2058321
version: 1
direction: direction/forward
top level: BBa_K180014
start: 988
end: 3952
orientation: inline

C0170
type: SequenceAnnotation
persistent identity: annotation2058341
display id: annotation2058341
version: 1
top level: BBa_K180014
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K180014/annotation2058341/range2058341/1

https://synbiohub.org/public/igem/BBa_K180014/annotation2058341/range2058341/1
type: Range
persistent identity: range2058341
display id: range2058341
version: 1
direction: direction/forward
top level: BBa_K180014
start: 3207
end: 3815
orientation: inline

B0034
type: SequenceAnnotation
persistent identity: annotation2058329
display id: annotation2058329
version: 1
top level: BBa_K180014
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K180014/annotation2058329/range2058329/1

https://synbiohub.org/public/igem/BBa_K180014/annotation2058329/range2058329/1
type: Range
persistent identity: range2058329
display id: range2058329
version: 1
direction: direction/forward
top level: BBa_K180014
start: 3189
end: 3200
orientation: inline

BBa_K113010
type: ComponentDefinition
persistent identity: BBa K113010
display id: BBa_K113010
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: overlapping T7 promoter
creator: Wu Jingjing, Wang Jinyu
created: 2008-10-25T11:00:00Z
modified: 2015-05-08T01:09:20Z
discontinued: false
dominant: false
experience: experience/None
group u list: _254_
m user id: 0
owner id: 2711
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Two T7 promoters are reversely overlapping.
mutable notes: It is difficult to make this short DNA by PCR as both sides are T7 promoters, which are very similar. It is highly possible that they form loops before they can be synthesized by PCR. Thus we order two single stranded DNA and then anneal them together by touchdown PCR.
mutable provenance: The sequence of T7 promoter is TAATACGACTCACTATA.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: T7 promoter
sequence: https://synbiohub.org/public/igem/BBa_K113010_sequence/1

T7 promoter
type: SequenceAnnotation
persistent identity: annotation1989277
display id: annotation1989277
version: 1
top level: BBa_K113010
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K113010/annotation1989277/range1989277/1

https://synbiohub.org/public/igem/BBa_K113010/annotation1989277/range1989277/1
type: Range
persistent identity: range1989277
display id: range1989277
version: 1
direction: direction/reverse
top level: BBa_K113010
start: 4
end: 21
orientation: inline

T7 promoter
type: SequenceAnnotation
persistent identity: annotation1989307
display id: annotation1989307
version: 1
top level: BBa_K113010
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K113010/annotation1989307/range1989307/1

https://synbiohub.org/public/igem/BBa_K113010/annotation1989307/range1989307/1
type: Range
persistent identity: range1989307
display id: range1989307
version: 1
direction: direction/forward
top level: BBa_K113010
start: 20
end: 37
orientation: inline

BBa_B0024
type: ComponentDefinition
persistent identity: BBa B0024
display id: BBa_B0024
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: double terminator (B0012-B0011), reversed
creator: Caitlin Conboy
created: 2003-12-02T12:00:00Z
modified: 2015-08-31T04:07:20Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence: https://synbiohub.org/public/igem/BBa_B0024_sequence/1

BBa_B0021
type: ComponentDefinition
persistent identity: BBa B0021
display id: BBa_B0021
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LuxICDABEG (+/-), reversed
creator: Caitlin Conboy
created: 2003-10-13T11:00:00Z
modified: 2015-08-31T04:07:20Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Bidirectional transcriptional terminator cloned in the reverse direction of B0011. 22 bp stem-loop
mutable notes: Cloned with primer-dimers into pSB1A2
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0021
sequence: https://synbiohub.org/public/igem/BBa_B0021_sequence/1

BBa_B0021
type: SequenceAnnotation
persistent identity: annotation7024
display id: annotation7024
version: 1
top level: BBa_B0021
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_B0021/annotation7024/range7024/1

https://synbiohub.org/public/igem/BBa_B0021/annotation7024/range7024/1
type: Range
persistent identity: range7024
display id: range7024
version: 1
direction: direction/forward
top level: BBa_B0021
start: 1
end: 46
orientation: inline

BBa_K598022
type: ComponentDefinition
persistent identity: BBa K598022
display id: BBa_K598022
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Bistable Switch Mutant 42
creator: Yan GONG, Shuobin CHEN, Yunpeng LIU
created: 2011-09-26T11:00:00Z
modified: 2015-05-08T01:12:50Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _770_
m user id: 0
owner id: 8817
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: The RBS of CI434 is CGAGACC in this bistble switch, which makes the RFP expresses three times as the GFP does in this bistable switch.
mutable notes: The library is constructed by mutagenesis.
mutable provenance: Engineered
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
sequence annotation: RBS
sequence annotation: PR (CI regulated promoter)
sequence annotation: PRM (CI434 regulated promoter)
sequence annotation: lamda CI repressor
sequence annotation: eGFP
sequence annotation: CI434 repressor
sequence annotation: mRFP
sequence: https://synbiohub.org/public/igem/BBa_K598022_sequence/1

RBS
type: SequenceAnnotation
persistent identity: annotation2150619
display id: annotation2150619
version: 1
top level: BBa_K598022
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K598022/annotation2150619/range2150619/1

https://synbiohub.org/public/igem/BBa_K598022/annotation2150619/range2150619/1
type: Range
persistent identity: range2150619
display id: range2150619
version: 1
direction: direction/forward
top level: BBa_K598022
start: 1556
end: 1567
orientation: inline

PR (CI regulated promoter)
type: SequenceAnnotation
persistent identity: annotation2150618
display id: annotation2150618
version: 1
top level: BBa_K598022
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K598022/annotation2150618/range2150618/1

https://synbiohub.org/public/igem/BBa_K598022/annotation2150618/range2150618/1
type: Range
persistent identity: range2150618
display id: range2150618
version: 1
direction: direction/forward
top level: BBa_K598022
start: 1492
end: 1540
orientation: inline

PRM (CI434 regulated promoter)
type: SequenceAnnotation
persistent identity: annotation2150617
display id: annotation2150617
version: 1
top level: BBa_K598022
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K598022/annotation2150617/range2150617/1

https://synbiohub.org/public/igem/BBa_K598022/annotation2150617/range2150617/1
type: Range
persistent identity: range2150617
display id: range2150617
version: 1
direction: direction/reverse
top level: BBa_K598022
start: 1440
end: 1491
orientation: inline

lamda CI repressor
type: SequenceAnnotation
persistent identity: annotation2150615
display id: annotation2150615
version: 1
top level: BBa_K598022
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K598022/annotation2150615/range2150615/1

https://synbiohub.org/public/igem/BBa_K598022/annotation2150615/range2150615/1
type: Range
persistent identity: range2150615
display id: range2150615
version: 1
direction: direction/reverse
top level: BBa_K598022
start: 708
end: 1421
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2150616
display id: annotation2150616
version: 1
top level: BBa_K598022
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K598022/annotation2150616/range2150616/1

https://synbiohub.org/public/igem/BBa_K598022/annotation2150616/range2150616/1
type: Range
persistent identity: range2150616
display id: range2150616
version: 1
direction: direction/reverse
top level: BBa_K598022
start: 1422
end: 1433
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2150621
display id: annotation2150621
version: 1
top level: BBa_K598022
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K598022/annotation2150621/range2150621/1

https://synbiohub.org/public/igem/BBa_K598022/annotation2150621/range2150621/1
type: Range
persistent identity: range2150621
display id: range2150621
version: 1
direction: direction/forward
top level: BBa_K598022
start: 2214
end: 2225
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2150614
display id: annotation2150614
version: 1
top level: BBa_K598022
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K598022/annotation2150614/range2150614/1

https://synbiohub.org/public/igem/BBa_K598022/annotation2150614/range2150614/1
type: Range
persistent identity: range2150614
display id: range2150614
version: 1
direction: direction/reverse
top level: BBa_K598022
start: 682
end: 693
orientation: inline

eGFP
type: SequenceAnnotation
persistent identity: annotation2150622
display id: annotation2150622
version: 1
top level: BBa_K598022
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K598022/annotation2150622/range2150622/1

https://synbiohub.org/public/igem/BBa_K598022/annotation2150622/range2150622/1
type: Range
persistent identity: range2150622
display id: range2150622
version: 1
direction: direction/forward
top level: BBa_K598022
start: 2226
end: 2945
orientation: inline

CI434 repressor
type: SequenceAnnotation
persistent identity: annotation2150620
display id: annotation2150620
version: 1
top level: BBa_K598022
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K598022/annotation2150620/range2150620/1

https://synbiohub.org/public/igem/BBa_K598022/annotation2150620/range2150620/1
type: Range
persistent identity: range2150620
display id: range2150620
version: 1
direction: direction/forward
top level: BBa_K598022
start: 1568
end: 2203
orientation: inline

mRFP
type: SequenceAnnotation
persistent identity: annotation2150613
display id: annotation2150613
version: 1
top level: BBa_K598022
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K598022/annotation2150613/range2150613/1

https://synbiohub.org/public/igem/BBa_K598022/annotation2150613/range2150613/1
type: Range
persistent identity: range2150613
display id: range2150613
version: 1
direction: direction/reverse
top level: BBa_K598022
start: 1
end: 681
orientation: inline

FlipFlop
type: ComponentDefinition
persistent identity: BBa K907002
display id: BBa_K907002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Binary signal generator, RBS(reverse) - attB - Promoter - attP - RBS
creator: Dong-hui Choe, Soo-in Lee
created: 2012-09-19T11:00:00Z
modified: 2015-05-08T01:13:44Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1172_
m user id: 0
owner id: 12628
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Dual phase protein generator promoter reversed, RBS(reverse) - attB - Promoter(reverse) - attP - RBS This part is composed of 3 elements. 1. RBS: BBa_B0034, upsteam one is reversed 2. Promoter: BBa_J23119, Bacterial constitutive promoter 3. att site: Recognition site for Mycobacteriophage Bxb1 integrase/excisionase We designed this part to generate two different proteins one at a time. At the first state, this device promotes transcription and translation of downstream gene because its promoter orientation. When Mycobacteriophage Bxb1 integrase recognizes and inverts sequence flanking with attB and attP sequence, promoter orientation reversed. Then this device promotes transcription and translation of upstream gene(must be reversed at the construction step). This device is tested by GFP and mRFP attached construct. See BBa_K090700N and BBa_K090700N
mutable notes: No
mutable provenance: Promoter and RBS are derived from BBa_J23119 and BBa_B0034, respectively. Whole construct was de novo synthesized by Bioneer.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
sequence annotation: BBa_J23119
sequence annotation: BBa_B0034, reverse
sequence annotation: BBa_B0034
sequence annotation: attB(Bxb1 Int)
sequence annotation: attP(Bxb1 Int)
sequence: https://synbiohub.org/public/igem/BBa_K907002_sequence/1

BBa_J23119
type: SequenceAnnotation
persistent identity: annotation2189785
display id: annotation2189785
version: 1
top level: FlipFlop
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K907002/annotation2189785/range2189785/1

https://synbiohub.org/public/igem/BBa_K907002/annotation2189785/range2189785/1
type: Range
persistent identity: range2189785
display id: range2189785
version: 1
direction: direction/forward
top level: FlipFlop
start: 69
end: 103
orientation: inline

BBa_B0034, reverse
type: SequenceAnnotation
persistent identity: annotation2188632
display id: annotation2188632
version: 1
top level: FlipFlop
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K907002/annotation2188632/range2188632/1

https://synbiohub.org/public/igem/BBa_K907002/annotation2188632/range2188632/1
type: Range
persistent identity: range2188632
display id: range2188632
version: 1
direction: direction/reverse
top level: FlipFlop
start: 1
end: 12
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2188633
display id: annotation2188633
version: 1
top level: FlipFlop
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K907002/annotation2188633/range2188633/1

https://synbiohub.org/public/igem/BBa_K907002/annotation2188633/range2188633/1
type: Range
persistent identity: range2188633
display id: range2188633
version: 1
direction: direction/forward
top level: FlipFlop
start: 163
end: 174
orientation: inline

attB(Bxb1 Int)
type: SequenceAnnotation
persistent identity: annotation2189782
display id: annotation2189782
version: 1
top level: FlipFlop
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K907002/annotation2189782/range2189782/1

https://synbiohub.org/public/igem/BBa_K907002/annotation2189782/range2189782/1
type: Range
persistent identity: range2189782
display id: range2189782
version: 1
direction: direction/forward
top level: FlipFlop
start: 19
end: 68
orientation: inline

attP(Bxb1 Int)
type: SequenceAnnotation
persistent identity: annotation2189784
display id: annotation2189784
version: 1
top level: FlipFlop
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K907002/annotation2189784/range2189784/1

https://synbiohub.org/public/igem/BBa_K907002/annotation2189784/range2189784/1
type: Range
persistent identity: range2189784
display id: range2189784
version: 1
direction: direction/forward
top level: FlipFlop
start: 104
end: 156
orientation: inline

BBa_B1009
type: ComponentDefinition
persistent identity: BBa B1009
display id: BBa_B1009
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Terminator (artificial, large, %T~=40%)
creator: Haiyao Huang
created: 2006-09-06T11:00:00Z
modified: 2015-08-31T04:07:21Z
discontinued: false
dominant: false
experience: experience/None
group u list: _41_
m user id: 0
owner id: 745
owning group id: 41
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Artificial terminator, estimated %T~>90% * 8bp stem, 6nt loop * Bidirectional, estimated reverse %T~>90%
mutable notes: Bidirectional, with the reverse estimated to be more effective than the forward. Has a polyA tail of 4 residues
mutable provenance: modified E. coli thr terminator, replaced all A-T pairs in stem with C-G pairs
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: PolyA
sequence annotation: B1009
sequence annotation: stem loop
sequence: https://synbiohub.org/public/igem/BBa_B1009_sequence/1

PolyA
type: SequenceAnnotation
persistent identity: annotation1901011
display id: annotation1901011
version: 1
top level: BBa_B1009
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_B1009/annotation1901011/range1901011/1

https://synbiohub.org/public/igem/BBa_B1009/annotation1901011/range1901011/1
type: Range
persistent identity: range1901011
display id: range1901011
version: 1
direction: direction/reverse
top level: BBa_B1009
start: 6
end: 9
orientation: inline

PolyA
type: SequenceAnnotation
persistent identity: annotation1901012
display id: annotation1901012
version: 1
top level: BBa_B1009
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_B1009/annotation1901012/range1901012/1

https://synbiohub.org/public/igem/BBa_B1009/annotation1901012/range1901012/1
type: Range
persistent identity: range1901012
display id: range1901012
version: 1
direction: direction/forward
top level: BBa_B1009
start: 32
end: 35
orientation: inline

B1009
type: SequenceAnnotation
persistent identity: annotation1901009
display id: annotation1901009
version: 1
top level: BBa_B1009
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_B1009/annotation1901009/range1901009/1

https://synbiohub.org/public/igem/BBa_B1009/annotation1901009/range1901009/1
type: Range
persistent identity: range1901009
display id: range1901009
version: 1
direction: direction/forward
top level: BBa_B1009
start: 1
end: 40
orientation: inline

stem loop
type: SequenceAnnotation
persistent identity: annotation1901010
display id: annotation1901010
version: 1
top level: BBa_B1009
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_B1009/annotation1901010/range1901010/1

https://synbiohub.org/public/igem/BBa_B1009/annotation1901010/range1901010/1
type: Range
persistent identity: range1901010
display id: range1901010
version: 1
direction: direction/forward
top level: BBa_B1009
start: 10
end: 31
orientation: inline

BBa_B1001
type: ComponentDefinition
persistent identity: BBa B1001
display id: BBa_B1001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Terminator (artifical, small, %T~=90)
creator: Haiyao Huang
created: 2006-08-29T11:00:00Z
modified: 2015-08-31T04:07:21Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _41_
m user id: 0
owner id: 745
owning group id: 41
sample status: In stock
status: status/Available
bookmark: false
mutable description: Artificial terminator, estimated %T>90
mutable notes: Poly T tail of 9 residues for high %T
mutable provenance: antiquity
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: PolyA tail
sequence annotation: stem loop
sequence annotation: Poly A tail
sequence annotation: B1001
sequence: https://synbiohub.org/public/igem/BBa_B1001_sequence/1

PolyA tail
type: SequenceAnnotation
persistent identity: annotation1898381
display id: annotation1898381
version: 1
top level: BBa_B1001
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_B1001/annotation1898381/range1898381/1

https://synbiohub.org/public/igem/BBa_B1001/annotation1898381/range1898381/1
type: Range
persistent identity: range1898381
display id: range1898381
version: 1
direction: direction/reverse
top level: BBa_B1001
start: 1
end: 9
orientation: inline

stem loop
type: SequenceAnnotation
persistent identity: annotation1898379
display id: annotation1898379
version: 1
top level: BBa_B1001
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_B1001/annotation1898379/range1898379/1

https://synbiohub.org/public/igem/BBa_B1001/annotation1898379/range1898379/1
type: Range
persistent identity: range1898379
display id: range1898379
version: 1
direction: direction/forward
top level: BBa_B1001
start: 10
end: 25
orientation: inline

Poly A tail
type: SequenceAnnotation
persistent identity: annotation1898380
display id: annotation1898380
version: 1
top level: BBa_B1001
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_B1001/annotation1898380/range1898380/1

https://synbiohub.org/public/igem/BBa_B1001/annotation1898380/range1898380/1
type: Range
persistent identity: range1898380
display id: range1898380
version: 1
direction: direction/reverse
top level: BBa_B1001
start: 26
end: 34
orientation: inline

B1001
type: SequenceAnnotation
persistent identity: annotation1898382
display id: annotation1898382
version: 1
top level: BBa_B1001
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_B1001/annotation1898382/range1898382/1

https://synbiohub.org/public/igem/BBa_B1001/annotation1898382/range1898382/1
type: Range
persistent identity: range1898382
display id: range1898382
version: 1
direction: direction/forward
top level: BBa_B1001
start: 1
end: 34
orientation: inline

BBa_J44002
type: ComponentDefinition
persistent identity: BBa J44002
display id: BBa_J44002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pBAD reverse
creator: Brad Ogden
created: 2006-08-15T11:00:00Z
modified: 2015-08-31T04:08:48Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _71_
m user id: 0
owner id: 811
owning group id: 71
sample status: In stock
status: status/Available
bookmark: true
mutable description: This is the pBAD promoter (BBa_I13453) in the opposite orientation. It can be used to drive transcription in the direction of suffix to prefix.
mutable notes: None.
mutable provenance: Cloned from synthetic oligonucleotides.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: promoter
sequence: https://synbiohub.org/public/igem/BBa_J44002_sequence/1

promoter
type: SequenceAnnotation
persistent identity: annotation2002776
display id: annotation2002776
version: 1
top level: BBa_J44002
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_J44002/annotation2002776/range2002776/1

https://synbiohub.org/public/igem/BBa_J44002/annotation2002776/range2002776/1
type: Range
persistent identity: range2002776
display id: range2002776
version: 1
direction: direction/reverse
top level: BBa_J44002
start: 1
end: 130
orientation: inline

BBa_K180011
type: ComponentDefinition
persistent identity: BBa K180011
display id: BBa_K180011
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Rock - Primary plasmid (part 1) [RhlR generator]
creator: Richard Wilson, Stephanie Chambers
created: 2009-10-18T11:00:00Z
modified: 2015-05-08T01:11:06Z
discontinued: false
dominant: false
experience: experience/None
group u list: _278_
m user id: 0
owner id: 4082
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Part of the iGEM2009 Team:Southampton rock-paper-scissors 'rock' primary plasmid.
mutable notes: Constitutive promoter selected to allow for continuous expression of C0171.
mutable provenance: See relevant basic biobrick parts (J23119, B0034, C0171, B0015)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0012
component: BBa_B0010
component: BBa_B0034
component: BBa_J23119
component: BBa_C0171
sequence annotation: BBa_B0012
sequence annotation: BBa_B0010
sequence annotation: BBa_C0171
sequence annotation: BBa_J23119
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K180011_sequence/1

BBa_B0012
type: Component
persistent identity: component2053908
display id: component2053908
version: 1
top level: BBa_K180011
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0010
type: Component
persistent identity: component2053906
display id: component2053906
version: 1
top level: BBa_K180011
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0034
type: Component
persistent identity: component2053904
display id: component2053904
version: 1
top level: BBa_K180011
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_J23119
type: Component
persistent identity: component2053902
display id: component2053902
version: 1
top level: BBa_K180011
access: public
definition: https://synbiohub.org/public/igem/BBa_J23119/1

BBa_C0171
type: Component
persistent identity: component2053905
display id: component2053905
version: 1
top level: BBa_K180011
access: public
definition: https://synbiohub.org/public/igem/BBa_C0171/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2053908
display id: annotation2053908
version: 1
top level: BBa_K180011
location: https://synbiohub.org/public/igem/BBa_K180011/annotation2053908/range2053908/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K180011/annotation2053908/range2053908/1
type: Range
persistent identity: range2053908
display id: range2053908
version: 1
direction: direction/forward
top level: BBa_K180011
start: 887
end: 927
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2053906
display id: annotation2053906
version: 1
top level: BBa_K180011
location: https://synbiohub.org/public/igem/BBa_K180011/annotation2053906/range2053906/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K180011/annotation2053906/range2053906/1
type: Range
persistent identity: range2053906
display id: range2053906
version: 1
direction: direction/forward
top level: BBa_K180011
start: 799
end: 878
orientation: inline

BBa_C0171
type: SequenceAnnotation
persistent identity: annotation2053905
display id: annotation2053905
version: 1
top level: BBa_K180011
location: https://synbiohub.org/public/igem/BBa_K180011/annotation2053905/range2053905/1
component: BBa_C0171

https://synbiohub.org/public/igem/BBa_K180011/annotation2053905/range2053905/1
type: Range
persistent identity: range2053905
display id: range2053905
version: 1
direction: direction/reverse
top level: BBa_K180011
start: 62
end: 790
orientation: inline

BBa_J23119
type: SequenceAnnotation
persistent identity: annotation2053902
display id: annotation2053902
version: 1
top level: BBa_K180011
location: https://synbiohub.org/public/igem/BBa_K180011/annotation2053902/range2053902/1
component: BBa_J23119

https://synbiohub.org/public/igem/BBa_K180011/annotation2053902/range2053902/1
type: Range
persistent identity: range2053902
display id: range2053902
version: 1
direction: direction/reverse
top level: BBa_K180011
start: 1
end: 35
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2053904
display id: annotation2053904
version: 1
top level: BBa_K180011
location: https://synbiohub.org/public/igem/BBa_K180011/annotation2053904/range2053904/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K180011/annotation2053904/range2053904/1
type: Range
persistent identity: range2053904
display id: range2053904
version: 1
direction: direction/reverse
top level: BBa_K180011
start: 44
end: 55
orientation: inline

BBa_I714889
type: ComponentDefinition
persistent identity: BBa I714889
display id: BBa_I714889
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: OR21 of PR and PRM
creator: Chunbo Lou
created: 2007-10-19T11:00:00Z
modified: 2015-08-31T04:07:48Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _142_
m user id: 0
owner id: 1911
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: we synthesize the OR promoter by replacing OR3 binding site of lambda CI with OR1 binding site of CI434.
mutable notes: making sure CI434 protein can repress CI's transcription.
mutable provenance: similar to lambda lysis/lysogenic regulatory region.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -10
sequence annotation: OR1_434
sequence annotation: -35
sequence: https://synbiohub.org/public/igem/BBa_I714889_sequence/1

-10
type: SequenceAnnotation
persistent identity: annotation1951424
display id: annotation1951424
version: 1
top level: BBa_I714889
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I714889/annotation1951424/range1951424/1

https://synbiohub.org/public/igem/BBa_I714889/annotation1951424/range1951424/1
type: Range
persistent identity: range1951424
display id: range1951424
version: 1
direction: direction/reverse
top level: BBa_I714889
start: 26
end: 31
orientation: inline

OR1_434
type: SequenceAnnotation
persistent identity: annotation1951383
display id: annotation1951383
version: 1
top level: BBa_I714889
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I714889/annotation1951383/range1951383/1

https://synbiohub.org/public/igem/BBa_I714889/annotation1951383/range1951383/1
type: Range
persistent identity: range1951383
display id: range1951383
version: 1
direction: direction/forward
top level: BBa_I714889
start: 1
end: 14
orientation: inline

OR1_434
type: SequenceAnnotation
persistent identity: annotation1951380
display id: annotation1951380
version: 1
top level: BBa_I714889
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I714889/annotation1951380/range1951380/1

https://synbiohub.org/public/igem/BBa_I714889/annotation1951380/range1951380/1
type: Range
persistent identity: range1951380
display id: range1951380
version: 1
direction: direction/forward
top level: BBa_I714889
start: 31
end: 44
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1951382
display id: annotation1951382
version: 1
top level: BBa_I714889
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I714889/annotation1951382/range1951382/1

https://synbiohub.org/public/igem/BBa_I714889/annotation1951382/range1951382/1
type: Range
persistent identity: range1951382
display id: range1951382
version: 1
direction: direction/reverse
top level: BBa_I714889
start: 49
end: 54
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1951425
display id: annotation1951425
version: 1
top level: BBa_I714889
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I714889/annotation1951425/range1951425/1

https://synbiohub.org/public/igem/BBa_I714889/annotation1951425/range1951425/1
type: Range
persistent identity: range1951425
display id: range1951425
version: 1
direction: direction/forward
top level: BBa_I714889
start: 67
end: 72
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1951381
display id: annotation1951381
version: 1
top level: BBa_I714889
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I714889/annotation1951381/range1951381/1

https://synbiohub.org/public/igem/BBa_I714889/annotation1951381/range1951381/1
type: Range
persistent identity: range1951381
display id: range1951381
version: 1
direction: direction/forward
top level: BBa_I714889
start: 89
end: 95
orientation: inline

BBa_B0014
type: ComponentDefinition
persistent identity: BBa B0014
display id: BBa_B0014
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: double terminator (B0012-B0011)
creator: Reshma Shetty
created: 2003-07-15T11:00:00Z
modified: 2015-08-31T04:07:20Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Double terminator consisting of BBa_B0012 and BBa_B0011
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000141
role: partType/Terminator
component: BBa_B0012
component: BBa_B0011
sequence annotation: BBa_B0012
sequence annotation: BBa_B0011
sequence: https://synbiohub.org/public/igem/BBa_B0014_sequence/1

BBa_B0012
type: Component
persistent identity: component939303
display id: component939303
version: 1
top level: BBa_B0014
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0011
type: Component
persistent identity: component939311
display id: component939311
version: 1
top level: BBa_B0014
access: public
definition: BBa_B0011

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation939303
display id: annotation939303
version: 1
top level: BBa_B0014
location: https://synbiohub.org/public/igem/BBa_B0014/annotation939303/range939303/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_B0014/annotation939303/range939303/1
type: Range
persistent identity: range939303
display id: range939303
version: 1
direction: direction/forward
top level: BBa_B0014
start: 1
end: 41
orientation: inline

BBa_B0011
type: SequenceAnnotation
persistent identity: annotation939311
display id: annotation939311
version: 1
top level: BBa_B0014
location: https://synbiohub.org/public/igem/BBa_B0014/annotation939311/range939311/1
component: BBa_B0011

https://synbiohub.org/public/igem/BBa_B0014/annotation939311/range939311/1
type: Range
persistent identity: range939311
display id: range939311
version: 1
direction: direction/forward
top level: BBa_B0014
start: 50
end: 95
orientation: inline

P3
type: ComponentDefinition
persistent identity: BBa K100002
display id: BBa_K100002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Edited Xylose Regulated Bi-Directional Operator 2
creator: Garrett Tobin
created: 2008-08-17T11:00:00Z
modified: 2015-05-08T01:08:41Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _213_
m user id: 0
owner id: 1752
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part contains the operator region controlling transcription for the xyl operon. This regulatory region is dependent upon the binding of CRP-cAMP and XylR bound to xylose for activation. Upon binding, transcription is activated in both directions (xylFGH and xylAB facing). This part has a scrambled CRP binding site and and altered RNA ploymerase binding site that was designed to more resemble the consensus sequence at an intermediate level.
mutable notes: Both directions were kept in the sequence of the part even though it is intended to work in the right facing direction because of possible looping that may occur as a transcriptional control. The RNA polymerase binding site was altered to make binding stronger, attempting to allow activation when XylR-xylose alone is bound (the absence of CRP-cAMP).
mutable provenance: Taken from E. Coli genome bases 3728830-3729092, base pairs changed at RNA polymerase bining sites.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: RNA poly. Binding Site
sequence annotation: Scrambled CRP Binding Site
sequence: https://synbiohub.org/public/igem/BBa_K100002_sequence/1

RNA poly. Binding Site
type: SequenceAnnotation
persistent identity: annotation1972260
display id: annotation1972260
version: 1
top level: P3
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K100002/annotation1972260/range1972260/1

https://synbiohub.org/public/igem/BBa_K100002/annotation1972260/range1972260/1
type: Range
persistent identity: range1972260
display id: range1972260
version: 1
direction: direction/reverse
top level: P3
start: 21
end: 26
orientation: inline

RNA poly. Binding Site
type: SequenceAnnotation
persistent identity: annotation1972264
display id: annotation1972264
version: 1
top level: P3
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K100002/annotation1972264/range1972264/1

https://synbiohub.org/public/igem/BBa_K100002/annotation1972264/range1972264/1
type: Range
persistent identity: range1972264
display id: range1972264
version: 1
direction: direction/forward
top level: P3
start: 243
end: 248
orientation: inline

Scrambled CRP Binding Site
type: SequenceAnnotation
persistent identity: annotation1972262
display id: annotation1972262
version: 1
top level: P3
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K100002/annotation1972262/range1972262/1

https://synbiohub.org/public/igem/BBa_K100002/annotation1972262/range1972262/1
type: Range
persistent identity: range1972262
display id: range1972262
version: 1
direction: direction/forward
top level: P3
start: 88
end: 104
orientation: inline

RNA poly. Binding Site
type: SequenceAnnotation
persistent identity: annotation1972259
display id: annotation1972259
version: 1
top level: P3
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K100002/annotation1972259/range1972259/1

https://synbiohub.org/public/igem/BBa_K100002/annotation1972259/range1972259/1
type: Range
persistent identity: range1972259
display id: range1972259
version: 1
direction: direction/forward
top level: P3
start: 45
end: 50
orientation: inline

RNA poly. Binding Site
type: SequenceAnnotation
persistent identity: annotation1972265
display id: annotation1972265
version: 1
top level: P3
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K100002/annotation1972265/range1972265/1

https://synbiohub.org/public/igem/BBa_K100002/annotation1972265/range1972265/1
type: Range
persistent identity: range1972265
display id: range1972265
version: 1
direction: direction/forward
top level: P3
start: 265
end: 270
orientation: inline

BBa_J31013
type: ComponentDefinition
persistent identity: BBa J31013
display id: BBa_J31013
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pLac Backwards [cf. BBa_R0010]
creator: Karmella Haynes
created: 2007-04-11T11:00:00Z
modified: 2015-08-31T04:08:45Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _61_
m user id: 0
owner id: 1144
owning group id: 61
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Promoter from the lack operon cloned in the reverse orientation.
mutable notes: Promoter from the lack operon cloned in the reverse orientation.
mutable provenance: BBa_R0010
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: end of LacI coding region (inactive)
sequence annotation: -35
sequence annotation: R0010 reverse
sequence annotation: -10
sequence annotation: LacI binding site
sequence annotation: CAP binding site
sequence: https://synbiohub.org/public/igem/BBa_J31013_sequence/1

end of LacI coding region (inactive)
type: SequenceAnnotation
persistent identity: annotation1928669
display id: annotation1928669
version: 1
top level: BBa_J31013
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J31013/annotation1928669/range1928669/1

https://synbiohub.org/public/igem/BBa_J31013/annotation1928669/range1928669/1
type: Range
persistent identity: range1928669
display id: range1928669
version: 1
direction: direction/reverse
top level: BBa_J31013
start: 113
end: 200
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1928671
display id: annotation1928671
version: 1
top level: BBa_J31013
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_J31013/annotation1928671/range1928671/1

https://synbiohub.org/public/igem/BBa_J31013/annotation1928671/range1928671/1
type: Range
persistent identity: range1928671
display id: range1928671
version: 1
direction: direction/reverse
top level: BBa_J31013
start: 58
end: 64
orientation: inline

R0010 reverse
type: SequenceAnnotation
persistent identity: annotation1928668
display id: annotation1928668
version: 1
top level: BBa_J31013
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_J31013/annotation1928668/range1928668/1

https://synbiohub.org/public/igem/BBa_J31013/annotation1928668/range1928668/1
type: Range
persistent identity: range1928668
display id: range1928668
version: 1
direction: direction/reverse
top level: BBa_J31013
start: 1
end: 200
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1928672
display id: annotation1928672
version: 1
top level: BBa_J31013
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_J31013/annotation1928672/range1928672/1

https://synbiohub.org/public/igem/BBa_J31013/annotation1928672/range1928672/1
type: Range
persistent identity: range1928672
display id: range1928672
version: 1
direction: direction/reverse
top level: BBa_J31013
start: 35
end: 40
orientation: inline

LacI binding site
type: SequenceAnnotation
persistent identity: annotation1928673
display id: annotation1928673
version: 1
top level: BBa_J31013
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_J31013/annotation1928673/range1928673/1

https://synbiohub.org/public/igem/BBa_J31013/annotation1928673/range1928673/1
type: Range
persistent identity: range1928673
display id: range1928673
version: 1
direction: direction/forward
top level: BBa_J31013
start: 1
end: 34
orientation: inline

CAP binding site
type: SequenceAnnotation
persistent identity: annotation1928670
display id: annotation1928670
version: 1
top level: BBa_J31013
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_J31013/annotation1928670/range1928670/1

https://synbiohub.org/public/igem/BBa_J31013/annotation1928670/range1928670/1
type: Range
persistent identity: range1928670
display id: range1928670
version: 1
direction: direction/forward
top level: BBa_J31013
start: 74
end: 112
orientation: inline

BBa_K180019
type: ComponentDefinition
persistent identity: BBa K180019
display id: BBa_K180019
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Scissors - Primary plasmid (part 2) [tac pR, mBanana and LuxI generator]
creator: Richard Wilson, Stephanie Chambers
created: 2009-10-18T11:00:00Z
modified: 2015-05-08T01:11:06Z
discontinued: false
dominant: false
experience: experience/None
group u list: _278_
m user id: 0
owner id: 4082
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Part of the iGEM2009 Team:Southampton rock-paper-scissors 'scissors' primary plasmid.
mutable notes: Regulated expression of LasI (C0178) and mBanana (K180009) by tac pR (K180000), controlled by presence of lac/IPTG or trp in the cell.
mutable provenance: See relevant basic biobrick parts (K180000, B0034, K180009, B0015).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0034
component: BBa_B0012
component: BBa_C0161
component: BBa_B0010
component: BBa_K180000
component: BBa_K180009
sequence annotation: BBa_C0161
sequence annotation: BBa_B0034
sequence annotation: BBa_K180009
sequence annotation: BBa_B0012
sequence annotation: BBa_B0010
sequence annotation: BBa_K180000
sequence: https://synbiohub.org/public/igem/BBa_K180019_sequence/1

BBa_B0034
type: Component
persistent identity: component2058407
display id: component2058407
version: 1
top level: BBa_K180019
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0012
type: Component
persistent identity: component2058412
display id: component2058412
version: 1
top level: BBa_K180019
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_C0161
type: Component
persistent identity: component2058409
display id: component2058409
version: 1
top level: BBa_K180019
access: public
definition: https://synbiohub.org/public/igem/BBa_C0161/1

BBa_B0010
type: Component
persistent identity: component2058410
display id: component2058410
version: 1
top level: BBa_K180019
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_K180000
type: Component
persistent identity: component2058401
display id: component2058401
version: 1
top level: BBa_K180019
access: public
definition: https://synbiohub.org/public/igem/BBa_K180000/1

BBa_B0034
type: Component
persistent identity: component2058403
display id: component2058403
version: 1
top level: BBa_K180019
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K180009
type: Component
persistent identity: component2058405
display id: component2058405
version: 1
top level: BBa_K180019
access: public
definition: https://synbiohub.org/public/igem/BBa_K180009/1

BBa_C0161
type: SequenceAnnotation
persistent identity: annotation2058409
display id: annotation2058409
version: 1
top level: BBa_K180019
location: https://synbiohub.org/public/igem/BBa_K180019/annotation2058409/range2058409/1
component: BBa_C0161

https://synbiohub.org/public/igem/BBa_K180019/annotation2058409/range2058409/1
type: Range
persistent identity: range2058409
display id: range2058409
version: 1
direction: direction/reverse
top level: BBa_K180019
start: 2208
end: 2792
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2058407
display id: annotation2058407
version: 1
top level: BBa_K180019
location: https://synbiohub.org/public/igem/BBa_K180019/annotation2058407/range2058407/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K180019/annotation2058407/range2058407/1
type: Range
persistent identity: range2058407
display id: range2058407
version: 1
direction: direction/reverse
top level: BBa_K180019
start: 2190
end: 2201
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2058403
display id: annotation2058403
version: 1
top level: BBa_K180019
location: https://synbiohub.org/public/igem/BBa_K180019/annotation2058403/range2058403/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K180019/annotation2058403/range2058403/1
type: Range
persistent identity: range2058403
display id: range2058403
version: 1
direction: direction/reverse
top level: BBa_K180019
start: 1456
end: 1467
orientation: inline

BBa_K180009
type: SequenceAnnotation
persistent identity: annotation2058405
display id: annotation2058405
version: 1
top level: BBa_K180019
location: https://synbiohub.org/public/igem/BBa_K180019/annotation2058405/range2058405/1
component: BBa_K180009

https://synbiohub.org/public/igem/BBa_K180019/annotation2058405/range2058405/1
type: Range
persistent identity: range2058405
display id: range2058405
version: 1
direction: direction/reverse
top level: BBa_K180019
start: 1474
end: 2181
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2058412
display id: annotation2058412
version: 1
top level: BBa_K180019
location: https://synbiohub.org/public/igem/BBa_K180019/annotation2058412/range2058412/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K180019/annotation2058412/range2058412/1
type: Range
persistent identity: range2058412
display id: range2058412
version: 1
direction: direction/forward
top level: BBa_K180019
start: 2889
end: 2929
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2058410
display id: annotation2058410
version: 1
top level: BBa_K180019
location: https://synbiohub.org/public/igem/BBa_K180019/annotation2058410/range2058410/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K180019/annotation2058410/range2058410/1
type: Range
persistent identity: range2058410
display id: range2058410
version: 1
direction: direction/forward
top level: BBa_K180019
start: 2801
end: 2880
orientation: inline

BBa_K180000
type: SequenceAnnotation
persistent identity: annotation2058401
display id: annotation2058401
version: 1
top level: BBa_K180019
location: https://synbiohub.org/public/igem/BBa_K180019/annotation2058401/range2058401/1
component: BBa_K180000

https://synbiohub.org/public/igem/BBa_K180019/annotation2058401/range2058401/1
type: Range
persistent identity: range2058401
display id: range2058401
version: 1
direction: direction/reverse
top level: BBa_K180019
start: 1
end: 1447
orientation: inline

BBa_K113011
type: ComponentDefinition
persistent identity: BBa K113011
display id: BBa_K113011
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: more overlapping T7 promoter
creator: Wu Jingjing, Wang Jinyu
created: 2008-10-25T11:00:00Z
modified: 2015-05-08T01:09:20Z
discontinued: false
dominant: false
experience: experience/None
group u list: _254_
m user id: 0
owner id: 2711
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Two T7 promoters are reversely overlapping in five base pairs, with the third one mutated from A to G.
mutable notes: It is difficult to make this short DNA by PCR as both sides are T7 promoters, which are very similar. It is highly possible that they form loops before they can be synthesized by PCR. Thus we order two single stranded DNA and then anneal them together by touchdown PCR.
mutable provenance: The sequence of T7 promoter is TAATACGACTCACTATA (from 5' to 3').
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: T7 promoter
sequence: https://synbiohub.org/public/igem/BBa_K113011_sequence/1

T7 promoter
type: SequenceAnnotation
persistent identity: annotation1989380
display id: annotation1989380
version: 1
top level: BBa_K113011
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K113011/annotation1989380/range1989380/1

https://synbiohub.org/public/igem/BBa_K113011/annotation1989380/range1989380/1
type: Range
persistent identity: range1989380
display id: range1989380
version: 1
direction: direction/forward
top level: BBa_K113011
start: 17
end: 34
orientation: inline

T7 promoter
type: SequenceAnnotation
persistent identity: annotation1989359
display id: annotation1989359
version: 1
top level: BBa_K113011
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K113011/annotation1989359/range1989359/1

https://synbiohub.org/public/igem/BBa_K113011/annotation1989359/range1989359/1
type: Range
persistent identity: range1989359
display id: range1989359
version: 1
direction: direction/reverse
top level: BBa_K113011
start: 4
end: 21
orientation: inline

GLF
type: ComponentDefinition
persistent identity: BBa K558005
display id: BBa_K558005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Zymomonas mobilis glucose facilitator gene
creator: Vadim Gladwill, Laura Colgin
created: 2011-09-19T11:00:00Z
modified: 2015-05-08T01:12:41Z
discontinued: false
dominant: false
experience: experience/None
group u list: _726_
m user id: 0
owner id: 6570
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
sequence annotation: BBa_B0014 Double Terminator
sequence annotation: GLF
sequence annotation: start
sequence annotation: RBS
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_K558005_sequence/1

BBa_B0014 Double Terminator
type: SequenceAnnotation
persistent identity: annotation2145002
display id: annotation2145002
version: 1
top level: GLF
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K558005/annotation2145002/range2145002/1

https://synbiohub.org/public/igem/BBa_K558005/annotation2145002/range2145002/1
type: Range
persistent identity: range2145002
display id: range2145002
version: 1
direction: direction/forward
top level: GLF
start: 1469
end: 1564
orientation: inline

GLF
type: SequenceAnnotation
persistent identity: annotation2145001
display id: annotation2145001
version: 1
top level: GLF
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K558005/annotation2145001/range2145001/1

https://synbiohub.org/public/igem/BBa_K558005/annotation2145001/range2145001/1
type: Range
persistent identity: range2145001
display id: range2145001
version: 1
direction: direction/forward
top level: GLF
start: 18
end: 1468
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2145030
display id: annotation2145030
version: 1
top level: GLF
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K558005/annotation2145030/range2145030/1

https://synbiohub.org/public/igem/BBa_K558005/annotation2145030/range2145030/1
type: Range
persistent identity: range2145030
display id: range2145030
version: 1
direction: direction/forward
top level: GLF
start: 18
end: 20
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2145000
display id: annotation2145000
version: 1
top level: GLF
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K558005/annotation2145000/range2145000/1

https://synbiohub.org/public/igem/BBa_K558005/annotation2145000/range2145000/1
type: Range
persistent identity: range2145000
display id: range2145000
version: 1
direction: direction/forward
top level: GLF
start: 1
end: 17
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2145031
display id: annotation2145031
version: 1
top level: GLF
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K558005/annotation2145031/range2145031/1

https://synbiohub.org/public/igem/BBa_K558005/annotation2145031/range2145031/1
type: Range
persistent identity: range2145031
display id: range2145031
version: 1
direction: direction/forward
top level: GLF
start: 1464
end: 1466
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2145032
display id: annotation2145032
version: 1
top level: GLF
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K558005/annotation2145032/range2145032/1

https://synbiohub.org/public/igem/BBa_K558005/annotation2145032/range2145032/1
type: Range
persistent identity: range2145032
display id: range2145032
version: 1
direction: direction/forward
top level: GLF
start: 1467
end: 1469
orientation: inline

BBa_K180015
type: ComponentDefinition
persistent identity: BBa K180015
display id: BBa_K180015
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Paper - Primary plasmid (part 2) [tac pR, GFP and RhlI generator]
creator: Richard Wilson, Stephanie Chambers
created: 2009-10-18T11:00:00Z
modified: 2015-05-08T01:11:06Z
discontinued: false
dominant: false
experience: experience/None
group u list: _278_
m user id: 0
owner id: 4082
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Part of the iGEM2009 Team:Southampton rock-paper-scissors 'rock' primary plasmid.
mutable notes: Regulated expression of RhlI (C0170) and GFP (E0040) by tac pR (K180000), controlled by presence of lac/IPTG or trp in the cell.
mutable provenance: See relevant basic biobrick parts (K180000, B0034, E0040, B0015).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_E0040
component: BBa_B0010
component: BBa_B0012
component: BBa_B0034
component: BBa_K180000
component: BBa_C0170
sequence annotation: BBa_B0010
sequence annotation: BBa_B0034
sequence annotation: BBa_C0170
sequence annotation: BBa_K180000
sequence annotation: BBa_B0012
sequence annotation: BBa_E0040
sequence: https://synbiohub.org/public/igem/BBa_K180015_sequence/1

BBa_E0040
type: Component
persistent identity: component2053962
display id: component2053962
version: 1
top level: BBa_K180015
access: public
definition: https://synbiohub.org/public/igem/BBa_E0040/1

BBa_B0010
type: Component
persistent identity: component2053967
display id: component2053967
version: 1
top level: BBa_K180015
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0012
type: Component
persistent identity: component2053969
display id: component2053969
version: 1
top level: BBa_K180015
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0034
type: Component
persistent identity: component2053964
display id: component2053964
version: 1
top level: BBa_K180015
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K180000
type: Component
persistent identity: component2053958
display id: component2053958
version: 1
top level: BBa_K180015
access: public
definition: https://synbiohub.org/public/igem/BBa_K180000/1

BBa_C0170
type: Component
persistent identity: component2053966
display id: component2053966
version: 1
top level: BBa_K180015
access: public
definition: https://synbiohub.org/public/igem/BBa_C0170/1

BBa_B0034
type: Component
persistent identity: component2053960
display id: component2053960
version: 1
top level: BBa_K180015
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2053967
display id: annotation2053967
version: 1
top level: BBa_K180015
location: https://synbiohub.org/public/igem/BBa_K180015/annotation2053967/range2053967/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K180015/annotation2053967/range2053967/1
type: Range
persistent identity: range2053967
display id: range2053967
version: 1
direction: direction/forward
top level: BBa_K180015
start: 2837
end: 2916
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2053964
display id: annotation2053964
version: 1
top level: BBa_K180015
location: https://synbiohub.org/public/igem/BBa_K180015/annotation2053964/range2053964/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K180015/annotation2053964/range2053964/1
type: Range
persistent identity: range2053964
display id: range2053964
version: 1
direction: direction/reverse
top level: BBa_K180015
start: 2202
end: 2213
orientation: inline

BBa_C0170
type: SequenceAnnotation
persistent identity: annotation2053966
display id: annotation2053966
version: 1
top level: BBa_K180015
location: https://synbiohub.org/public/igem/BBa_K180015/annotation2053966/range2053966/1
component: BBa_C0170

https://synbiohub.org/public/igem/BBa_K180015/annotation2053966/range2053966/1
type: Range
persistent identity: range2053966
display id: range2053966
version: 1
direction: direction/reverse
top level: BBa_K180015
start: 2220
end: 2828
orientation: inline

BBa_K180000
type: SequenceAnnotation
persistent identity: annotation2053958
display id: annotation2053958
version: 1
top level: BBa_K180015
location: https://synbiohub.org/public/igem/BBa_K180015/annotation2053958/range2053958/1
component: BBa_K180000

https://synbiohub.org/public/igem/BBa_K180015/annotation2053958/range2053958/1
type: Range
persistent identity: range2053958
display id: range2053958
version: 1
direction: direction/reverse
top level: BBa_K180015
start: 1
end: 1447
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2053969
display id: annotation2053969
version: 1
top level: BBa_K180015
location: https://synbiohub.org/public/igem/BBa_K180015/annotation2053969/range2053969/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K180015/annotation2053969/range2053969/1
type: Range
persistent identity: range2053969
display id: range2053969
version: 1
direction: direction/forward
top level: BBa_K180015
start: 2925
end: 2965
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2053960
display id: annotation2053960
version: 1
top level: BBa_K180015
location: https://synbiohub.org/public/igem/BBa_K180015/annotation2053960/range2053960/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K180015/annotation2053960/range2053960/1
type: Range
persistent identity: range2053960
display id: range2053960
version: 1
direction: direction/reverse
top level: BBa_K180015
start: 1456
end: 1467
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation2053962
display id: annotation2053962
version: 1
top level: BBa_K180015
location: https://synbiohub.org/public/igem/BBa_K180015/annotation2053962/range2053962/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_K180015/annotation2053962/range2053962/1
type: Range
persistent identity: range2053962
display id: range2053962
version: 1
direction: direction/reverse
top level: BBa_K180015
start: 1474
end: 2193
orientation: inline

BBa_K180006
type: ComponentDefinition
persistent identity: BBa K180006
display id: BBa_K180006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Game of Life - Primary plasmid (part 2) [lux pR, GFP and LacI generator]
creator: Richard Wilson, Stephanie Chambers
created: 2009-10-15T11:00:00Z
modified: 2015-05-08T01:11:06Z
discontinued: false
dominant: false
experience: experience/None
group u list: _278_
m user id: 0
owner id: 4082
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Part of the iGEM2009 Team:Southampton Game of Life primary plasmid.
mutable notes: Regulated expression of LacI (C0012) and GFP (E0040) by PLUX promoter (R0062), controlled by presence/absence of AHL-LuxR.
mutable provenance: See relevant basic biobrick parts (R0062, C0012, B0034, K180001, B0015)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_R0062
component: BBa_C0012
component: BBa_B0015
component: BBa_B0034
component: BBa_K180001
sequence annotation: BBa_B0034
sequence annotation: BBa_B0015
sequence annotation: BBa_R0062
sequence annotation: BBa_C0012
sequence annotation: BBa_K180001
sequence: https://synbiohub.org/public/igem/BBa_K180006_sequence/1

BBa_R0062
type: Component
persistent identity: component2267822
display id: component2267822
version: 1
top level: BBa_K180006
access: public
definition: https://synbiohub.org/public/igem/BBa_R0062/1

BBa_C0012
type: Component
persistent identity: component2267830
display id: component2267830
version: 1
top level: BBa_K180006
access: public
definition: https://synbiohub.org/public/igem/BBa_C0012/1

BBa_B0015
type: Component
persistent identity: component2267845
display id: component2267845
version: 1
top level: BBa_K180006
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_B0034
type: Component
persistent identity: component2267828
display id: component2267828
version: 1
top level: BBa_K180006
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K180001
type: Component
persistent identity: component2267838
display id: component2267838
version: 1
top level: BBa_K180006
access: public
definition: https://synbiohub.org/public/igem/BBa_K180001/1

BBa_B0034
type: Component
persistent identity: component2267834
display id: component2267834
version: 1
top level: BBa_K180006
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2267834
display id: annotation2267834
version: 1
top level: BBa_K180006
location: https://synbiohub.org/public/igem/BBa_K180006/annotation2267834/range2267834/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K180006/annotation2267834/range2267834/1
type: Range
persistent identity: range2267834
display id: range2267834
version: 1
direction: direction/reverse
top level: BBa_K180006
start: 1243
end: 1254
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2267828
display id: annotation2267828
version: 1
top level: BBa_K180006
location: https://synbiohub.org/public/igem/BBa_K180006/annotation2267828/range2267828/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K180006/annotation2267828/range2267828/1
type: Range
persistent identity: range2267828
display id: range2267828
version: 1
direction: direction/reverse
top level: BBa_K180006
start: 64
end: 75
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2267845
display id: annotation2267845
version: 1
top level: BBa_K180006
location: https://synbiohub.org/public/igem/BBa_K180006/annotation2267845/range2267845/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K180006/annotation2267845/range2267845/1
type: Range
persistent identity: range2267845
display id: range2267845
version: 1
direction: direction/reverse
top level: BBa_K180006
start: 2023
end: 2151
orientation: inline

BBa_R0062
type: SequenceAnnotation
persistent identity: annotation2267822
display id: annotation2267822
version: 1
top level: BBa_K180006
location: https://synbiohub.org/public/igem/BBa_K180006/annotation2267822/range2267822/1
component: BBa_R0062

https://synbiohub.org/public/igem/BBa_K180006/annotation2267822/range2267822/1
type: Range
persistent identity: range2267822
display id: range2267822
version: 1
direction: direction/forward
top level: BBa_K180006
start: 1
end: 55
orientation: inline

BBa_C0012
type: SequenceAnnotation
persistent identity: annotation2267830
display id: annotation2267830
version: 1
top level: BBa_K180006
location: https://synbiohub.org/public/igem/BBa_K180006/annotation2267830/range2267830/1
component: BBa_C0012

https://synbiohub.org/public/igem/BBa_K180006/annotation2267830/range2267830/1
type: Range
persistent identity: range2267830
display id: range2267830
version: 1
direction: direction/forward
top level: BBa_K180006
start: 82
end: 1209
orientation: inline

BBa_K180001
type: SequenceAnnotation
persistent identity: annotation2267838
display id: annotation2267838
version: 1
top level: BBa_K180006
location: https://synbiohub.org/public/igem/BBa_K180006/annotation2267838/range2267838/1
component: BBa_K180001

https://synbiohub.org/public/igem/BBa_K180006/annotation2267838/range2267838/1
type: Range
persistent identity: range2267838
display id: range2267838
version: 1
direction: direction/reverse
top level: BBa_K180006
start: 1261
end: 2014
orientation: inline

BBa_B0050
type: ComponentDefinition
persistent identity: BBa B0050
display id: BBa_B0050
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Terminator (pBR322, +/-)
creator: Caitlin Conboy
created: 2004-01-29T12:00:00Z
modified: 2015-08-31T04:07:20Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Bi-directional Terminator derived from pBR322.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0050_sequence/1

stem_loop
type: SequenceAnnotation
persistent identity: annotation318591
display id: annotation318591
version: 1
top level: BBa_B0050
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_B0050/annotation318591/range318591/1

https://synbiohub.org/public/igem/BBa_B0050/annotation318591/range318591/1
type: Range
persistent identity: range318591
display id: range318591
version: 1
direction: direction/forward
top level: BBa_B0050
start: 8
end: 24
orientation: inline

revPlam
type: ComponentDefinition
persistent identity: BBa I742126
display id: BBa_I742126
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Reverse lambda cI-regulated promoter
creator: Caroline Dahl
created: 2007-10-21T11:00:00Z
modified: 2015-08-31T04:08:02Z
discontinued: false
dominant: false
experience: experience/None
group u list: _123_
m user id: 0
owner id: 1968
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is an exact 'upside down' version of BBa_R0051. The promoter has two DNA binding sites for lambda cI repressor BBa_C0051. By adding this part, we wish to emphasise the possibility of engineering both DNA strands, and the additional regulatory potential that it gives.
mutable notes: Any 'upside down' (reverse complementary) sequences made by PCR amplification need be made with suffix and prefix is opposite order with respect to the original default sequence.
mutable provenance: See BBa_R0051.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I742126_sequence/1

BBa_K180010
type: ComponentDefinition
persistent identity: BBa K180010
display id: BBa_K180010
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Rock-paper-scissors - Rock primary plasmid
creator: Richard Wilson, Stephanie Chambers
created: 2009-10-20T11:00:00Z
modified: 2015-05-08T01:11:06Z
discontinued: false
dominant: false
experience: experience/None
group u list: _278_
m user id: 0
owner id: 4082
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: Composite part, consisting of BBa_K180011 and BBa_K180012, but with BBa_K180011 reading along reverse strand, and BBa_K180012 reading along the forward strand. Forms the 'rock' primary plasmid in Team:Southampton's rock-paper-scissors project design.
mutable notes: This part has been specified as a basic BioBrick, due to some of the component parts reading in reverse (unable to specify using the component BioBrick format).
mutable provenance: See relevant biobrick component parts (BBa_K180011 and BBa_K180012).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: B0034
sequence annotation: conserved
sequence annotation: C0178
sequence annotation: mCherry
sequence annotation: lacIq
sequence annotation: J23119
sequence annotation: -35
sequence annotation: B0015
sequence annotation: lasI
sequence annotation: rhlR
sequence annotation: Ptac
sequence annotation: K180000
sequence annotation: K180011
sequence annotation: K180012
sequence annotation: C0171
sequence annotation: -10
sequence annotation: K180008
sequence: https://synbiohub.org/public/igem/BBa_K180010_sequence/1

B0034
type: SequenceAnnotation
persistent identity: annotation2058302
display id: annotation2058302
version: 1
top level: BBa_K180010
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K180010/annotation2058302/range2058302/1

https://synbiohub.org/public/igem/BBa_K180010/annotation2058302/range2058302/1
type: Range
persistent identity: range2058302
display id: range2058302
version: 1
direction: direction/reverse
top level: BBa_K180010
start: 873
end: 884
orientation: inline

conserved
type: SequenceAnnotation
persistent identity: annotation2058300
display id: annotation2058300
version: 1
top level: BBa_K180010
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K180010/annotation2058300/range2058300/1

https://synbiohub.org/public/igem/BBa_K180010/annotation2058300/range2058300/1
type: Range
persistent identity: range2058300
display id: range2058300
version: 1
direction: direction/forward
top level: BBa_K180010
start: 2395
end: 2398
orientation: inline

C0178
type: SequenceAnnotation
persistent identity: annotation2058313
display id: annotation2058313
version: 1
top level: BBa_K180010
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K180010/annotation2058313/range2058313/1

https://synbiohub.org/public/igem/BBa_K180010/annotation2058313/range2058313/1
type: Range
persistent identity: range2058313
display id: range2058313
version: 1
direction: direction/forward
top level: BBa_K180010
start: 3143
end: 3751
orientation: inline

mCherry
type: SequenceAnnotation
persistent identity: annotation2058311
display id: annotation2058311
version: 1
top level: BBa_K180010
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K180010/annotation2058311/range2058311/1

https://synbiohub.org/public/igem/BBa_K180010/annotation2058311/range2058311/1
type: Range
persistent identity: range2058311
display id: range2058311
version: 1
direction: direction/forward
top level: BBa_K180010
start: 2409
end: 3116
orientation: inline

conserved
type: SequenceAnnotation
persistent identity: annotation2058301
display id: annotation2058301
version: 1
top level: BBa_K180010
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K180010/annotation2058301/range2058301/1

https://synbiohub.org/public/igem/BBa_K180010/annotation2058301/range2058301/1
type: Range
persistent identity: range2058301
display id: range2058301
version: 1
direction: direction/forward
top level: BBa_K180010
start: 3129
end: 3132
orientation: inline

B0034
type: SequenceAnnotation
persistent identity: annotation2058291
display id: annotation2058291
version: 1
top level: BBa_K180010
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K180010/annotation2058291/range2058291/1

https://synbiohub.org/public/igem/BBa_K180010/annotation2058291/range2058291/1
type: Range
persistent identity: range2058291
display id: range2058291
version: 1
direction: direction/forward
top level: BBa_K180010
start: 2391
end: 2402
orientation: inline

lacIq
type: SequenceAnnotation
persistent identity: annotation2058308
display id: annotation2058308
version: 1
top level: BBa_K180010
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K180010/annotation2058308/range2058308/1

https://synbiohub.org/public/igem/BBa_K180010/annotation2058308/range2058308/1
type: Range
persistent identity: range2058308
display id: range2058308
version: 1
direction: direction/forward
top level: BBa_K180010
start: 936
end: 2017
orientation: inline

J23119
type: SequenceAnnotation
persistent identity: annotation2058304
display id: annotation2058304
version: 1
top level: BBa_K180010
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K180010/annotation2058304/range2058304/1

https://synbiohub.org/public/igem/BBa_K180010/annotation2058304/range2058304/1
type: Range
persistent identity: range2058304
display id: range2058304
version: 1
direction: direction/reverse
top level: BBa_K180010
start: 893
end: 927
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2058305
display id: annotation2058305
version: 1
top level: BBa_K180010
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K180010/annotation2058305/range2058305/1

https://synbiohub.org/public/igem/BBa_K180010/annotation2058305/range2058305/1
type: Range
persistent identity: range2058305
display id: range2058305
version: 1
direction: direction/reverse
top level: BBa_K180010
start: 922
end: 927
orientation: inline

B0015
type: SequenceAnnotation
persistent identity: annotation2058288
display id: annotation2058288
version: 1
top level: BBa_K180010
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K180010/annotation2058288/range2058288/1

https://synbiohub.org/public/igem/BBa_K180010/annotation2058288/range2058288/1
type: Range
persistent identity: range2058288
display id: range2058288
version: 1
direction: direction/reverse
top level: BBa_K180010
start: 1
end: 129
orientation: inline

lasI
type: SequenceAnnotation
persistent identity: annotation2058314
display id: annotation2058314
version: 1
top level: BBa_K180010
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K180010/annotation2058314/range2058314/1

https://synbiohub.org/public/igem/BBa_K180010/annotation2058314/range2058314/1
type: Range
persistent identity: range2058314
display id: range2058314
version: 1
direction: direction/forward
top level: BBa_K180010
start: 3143
end: 3751
orientation: inline

rhlR
type: SequenceAnnotation
persistent identity: annotation2058312
display id: annotation2058312
version: 1
top level: BBa_K180010
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K180010/annotation2058312/range2058312/1

https://synbiohub.org/public/igem/BBa_K180010/annotation2058312/range2058312/1
type: Range
persistent identity: range2058312
display id: range2058312
version: 1
direction: direction/reverse
top level: BBa_K180010
start: 138
end: 866
orientation: inline

B0015
type: SequenceAnnotation
persistent identity: annotation2058315
display id: annotation2058315
version: 1
top level: BBa_K180010
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K180010/annotation2058315/range2058315/1

https://synbiohub.org/public/igem/BBa_K180010/annotation2058315/range2058315/1
type: Range
persistent identity: range2058315
display id: range2058315
version: 1
direction: direction/forward
top level: BBa_K180010
start: 3760
end: 3888
orientation: inline

Ptac
type: SequenceAnnotation
persistent identity: annotation2058309
display id: annotation2058309
version: 1
top level: BBa_K180010
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K180010/annotation2058309/range2058309/1

https://synbiohub.org/public/igem/BBa_K180010/annotation2058309/range2058309/1
type: Range
persistent identity: range2058309
display id: range2058309
version: 1
direction: direction/forward
top level: BBa_K180010
start: 2260
end: 2287
orientation: inline

K180000
type: SequenceAnnotation
persistent identity: annotation2058307
display id: annotation2058307
version: 1
top level: BBa_K180010
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K180010/annotation2058307/range2058307/1

https://synbiohub.org/public/igem/BBa_K180010/annotation2058307/range2058307/1
type: Range
persistent identity: range2058307
display id: range2058307
version: 1
direction: direction/forward
top level: BBa_K180010
start: 936
end: 2382
orientation: inline

K180011
type: SequenceAnnotation
persistent identity: annotation2058286
display id: annotation2058286
version: 1
top level: BBa_K180010
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K180010/annotation2058286/range2058286/1

https://synbiohub.org/public/igem/BBa_K180010/annotation2058286/range2058286/1
type: Range
persistent identity: range2058286
display id: range2058286
version: 1
direction: direction/reverse
top level: BBa_K180010
start: 1
end: 927
orientation: inline

K180012
type: SequenceAnnotation
persistent identity: annotation2058287
display id: annotation2058287
version: 1
top level: BBa_K180010
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K180010/annotation2058287/range2058287/1

https://synbiohub.org/public/igem/BBa_K180010/annotation2058287/range2058287/1
type: Range
persistent identity: range2058287
display id: range2058287
version: 1
direction: direction/forward
top level: BBa_K180010
start: 936
end: 3888
orientation: inline

B0034
type: SequenceAnnotation
persistent identity: annotation2058292
display id: annotation2058292
version: 1
top level: BBa_K180010
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K180010/annotation2058292/range2058292/1

https://synbiohub.org/public/igem/BBa_K180010/annotation2058292/range2058292/1
type: Range
persistent identity: range2058292
display id: range2058292
version: 1
direction: direction/forward
top level: BBa_K180010
start: 3125
end: 3136
orientation: inline

conserved
type: SequenceAnnotation
persistent identity: annotation2058303
display id: annotation2058303
version: 1
top level: BBa_K180010
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K180010/annotation2058303/range2058303/1

https://synbiohub.org/public/igem/BBa_K180010/annotation2058303/range2058303/1
type: Range
persistent identity: range2058303
display id: range2058303
version: 1
direction: direction/reverse
top level: BBa_K180010
start: 877
end: 880
orientation: inline

C0171
type: SequenceAnnotation
persistent identity: annotation2058289
display id: annotation2058289
version: 1
top level: BBa_K180010
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K180010/annotation2058289/range2058289/1

https://synbiohub.org/public/igem/BBa_K180010/annotation2058289/range2058289/1
type: Range
persistent identity: range2058289
display id: range2058289
version: 1
direction: direction/reverse
top level: BBa_K180010
start: 138
end: 866
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2058306
display id: annotation2058306
version: 1
top level: BBa_K180010
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K180010/annotation2058306/range2058306/1

https://synbiohub.org/public/igem/BBa_K180010/annotation2058306/range2058306/1
type: Range
persistent identity: range2058306
display id: range2058306
version: 1
direction: direction/reverse
top level: BBa_K180010
start: 899
end: 904
orientation: inline

K180008
type: SequenceAnnotation
persistent identity: annotation2058310
display id: annotation2058310
version: 1
top level: BBa_K180010
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K180010/annotation2058310/range2058310/1

https://synbiohub.org/public/igem/BBa_K180010/annotation2058310/range2058310/1
type: Range
persistent identity: range2058310
display id: range2058310
version: 1
direction: direction/forward
top level: BBa_K180010
start: 2409
end: 3116
orientation: inline

BBa_B1007
type: ComponentDefinition
persistent identity: BBa B1007
display id: BBa_B1007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Terminator (artificial, large, %T~=80)
creator: Haiyao Huang
created: 2006-09-06T11:00:00Z
modified: 2015-08-31T04:07:21Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _41_
m user id: 0
owner id: 745
owning group id: 41
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Artificial terminator, estimated %T~=80% # 8bp stem, 6nt loop # Bidirectional, estimated reverse %T~>90%
mutable notes: Bidirectional, with the reverse estimated to be more effective than the forward. Has a polyA tail of 6 residues.
mutable provenance: modified E. coli thr terminator, replaced all A-T pairs in stem with C-G pairs
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: PolyA
sequence annotation: modified thr
sequence annotation: B1007
sequence: https://synbiohub.org/public/igem/BBa_B1007_sequence/1

PolyA
type: SequenceAnnotation
persistent identity: annotation1900958
display id: annotation1900958
version: 1
top level: BBa_B1007
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_B1007/annotation1900958/range1900958/1

https://synbiohub.org/public/igem/BBa_B1007/annotation1900958/range1900958/1
type: Range
persistent identity: range1900958
display id: range1900958
version: 1
direction: direction/forward
top level: BBa_B1007
start: 32
end: 37
orientation: inline

modified thr
type: SequenceAnnotation
persistent identity: annotation1900956
display id: annotation1900956
version: 1
top level: BBa_B1007
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_B1007/annotation1900956/range1900956/1

https://synbiohub.org/public/igem/BBa_B1007/annotation1900956/range1900956/1
type: Range
persistent identity: range1900956
display id: range1900956
version: 1
direction: direction/forward
top level: BBa_B1007
start: 10
end: 31
orientation: inline

PolyA
type: SequenceAnnotation
persistent identity: annotation1900957
display id: annotation1900957
version: 1
top level: BBa_B1007
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_B1007/annotation1900957/range1900957/1

https://synbiohub.org/public/igem/BBa_B1007/annotation1900957/range1900957/1
type: Range
persistent identity: range1900957
display id: range1900957
version: 1
direction: direction/reverse
top level: BBa_B1007
start: 4
end: 9
orientation: inline

B1007
type: SequenceAnnotation
persistent identity: annotation1900955
display id: annotation1900955
version: 1
top level: BBa_B1007
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_B1007/annotation1900955/range1900955/1

https://synbiohub.org/public/igem/BBa_B1007/annotation1900955/range1900955/1
type: Range
persistent identity: range1900955
display id: range1900955
version: 1
direction: direction/forward
top level: BBa_B1007
start: 1
end: 40
orientation: inline

BBa_K598002
type: ComponentDefinition
persistent identity: BBa K598002
display id: BBa_K598002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Bistable Switch Mutant 68
creator: Yan GONG, Shuobin CHEN, Yunpeng LIU, Xiaowei YAN
created: 2011-09-26T11:00:00Z
modified: 2015-05-08T01:12:50Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _770_
m user id: 0
owner id: 8683
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: RFP+RBS_SDA-C1+OR21 double directed promoter+SDcro CI434+eGFP
mutable notes: mutate the RBS seqence change the translation rate of CI434 to get the appropriate ratio of green and red.
mutable provenance: The part is made up of the standard parts from partsregistry, and some of the sub-parts are inverted by PCR.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
sequence annotation: RBS
sequence annotation: lamda CI repressor
sequence annotation: PR (CI regulated promoter)
sequence annotation: CI434 repressor
sequence annotation: PRM (CI434 regulated promoter)
sequence annotation: eGFP
sequence annotation: mRFP
sequence: https://synbiohub.org/public/igem/BBa_K598002_sequence/1

RBS
type: SequenceAnnotation
persistent identity: annotation2150247
display id: annotation2150247
version: 1
top level: BBa_K598002
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K598002/annotation2150247/range2150247/1

https://synbiohub.org/public/igem/BBa_K598002/annotation2150247/range2150247/1
type: Range
persistent identity: range2150247
display id: range2150247
version: 1
direction: direction/forward
top level: BBa_K598002
start: 1556
end: 1567
orientation: inline

lamda CI repressor
type: SequenceAnnotation
persistent identity: annotation2150238
display id: annotation2150238
version: 1
top level: BBa_K598002
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K598002/annotation2150238/range2150238/1

https://synbiohub.org/public/igem/BBa_K598002/annotation2150238/range2150238/1
type: Range
persistent identity: range2150238
display id: range2150238
version: 1
direction: direction/reverse
top level: BBa_K598002
start: 708
end: 1421
orientation: inline

PR (CI regulated promoter)
type: SequenceAnnotation
persistent identity: annotation2150246
display id: annotation2150246
version: 1
top level: BBa_K598002
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K598002/annotation2150246/range2150246/1

https://synbiohub.org/public/igem/BBa_K598002/annotation2150246/range2150246/1
type: Range
persistent identity: range2150246
display id: range2150246
version: 1
direction: direction/forward
top level: BBa_K598002
start: 1492
end: 1540
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2150237
display id: annotation2150237
version: 1
top level: BBa_K598002
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K598002/annotation2150237/range2150237/1

https://synbiohub.org/public/igem/BBa_K598002/annotation2150237/range2150237/1
type: Range
persistent identity: range2150237
display id: range2150237
version: 1
direction: direction/reverse
top level: BBa_K598002
start: 682
end: 693
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2150249
display id: annotation2150249
version: 1
top level: BBa_K598002
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K598002/annotation2150249/range2150249/1

https://synbiohub.org/public/igem/BBa_K598002/annotation2150249/range2150249/1
type: Range
persistent identity: range2150249
display id: range2150249
version: 1
direction: direction/forward
top level: BBa_K598002
start: 2214
end: 2225
orientation: inline

CI434 repressor
type: SequenceAnnotation
persistent identity: annotation2150248
display id: annotation2150248
version: 1
top level: BBa_K598002
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K598002/annotation2150248/range2150248/1

https://synbiohub.org/public/igem/BBa_K598002/annotation2150248/range2150248/1
type: Range
persistent identity: range2150248
display id: range2150248
version: 1
direction: direction/forward
top level: BBa_K598002
start: 1568
end: 2203
orientation: inline

PRM (CI434 regulated promoter)
type: SequenceAnnotation
persistent identity: annotation2150243
display id: annotation2150243
version: 1
top level: BBa_K598002
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K598002/annotation2150243/range2150243/1

https://synbiohub.org/public/igem/BBa_K598002/annotation2150243/range2150243/1
type: Range
persistent identity: range2150243
display id: range2150243
version: 1
direction: direction/reverse
top level: BBa_K598002
start: 1440
end: 1491
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2150239
display id: annotation2150239
version: 1
top level: BBa_K598002
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K598002/annotation2150239/range2150239/1

https://synbiohub.org/public/igem/BBa_K598002/annotation2150239/range2150239/1
type: Range
persistent identity: range2150239
display id: range2150239
version: 1
direction: direction/reverse
top level: BBa_K598002
start: 1422
end: 1433
orientation: inline

eGFP
type: SequenceAnnotation
persistent identity: annotation2150250
display id: annotation2150250
version: 1
top level: BBa_K598002
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K598002/annotation2150250/range2150250/1

https://synbiohub.org/public/igem/BBa_K598002/annotation2150250/range2150250/1
type: Range
persistent identity: range2150250
display id: range2150250
version: 1
direction: direction/forward
top level: BBa_K598002
start: 2226
end: 2945
orientation: inline

mRFP
type: SequenceAnnotation
persistent identity: annotation2150236
display id: annotation2150236
version: 1
top level: BBa_K598002
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K598002/annotation2150236/range2150236/1

https://synbiohub.org/public/igem/BBa_K598002/annotation2150236/range2150236/1
type: Range
persistent identity: range2150236
display id: range2150236
version: 1
direction: direction/reverse
top level: BBa_K598002
start: 1
end: 681
orientation: inline

BBa_B1008
type: ComponentDefinition
persistent identity: BBa B1008
display id: BBa_B1008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Terminator (artificial, large, %T~=70)
creator: Haiyao Huang
created: 2006-09-06T11:00:00Z
modified: 2015-08-31T04:07:21Z
discontinued: false
dominant: false
experience: experience/None
group u list: _41_
m user id: 0
owner id: 745
owning group id: 41
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Artificial terminator, estimated %T~=70% *8bp stem, 6nt loop *Bidirectional, estimated reverse %T~=80%
mutable notes: Bidirectional, with the reverse estimated to be more effective than the forward. Has a polyA tail of 5 residues.
mutable provenance: modified E. coli thr terminator, replaced all A-T pairs in stem with C-G pair
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: B1008
sequence annotation: PolyA
sequence annotation: modified thr terminator
sequence: https://synbiohub.org/public/igem/BBa_B1008_sequence/1

B1008
type: SequenceAnnotation
persistent identity: annotation1901005
display id: annotation1901005
version: 1
top level: BBa_B1008
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_B1008/annotation1901005/range1901005/1

https://synbiohub.org/public/igem/BBa_B1008/annotation1901005/range1901005/1
type: Range
persistent identity: range1901005
display id: range1901005
version: 1
direction: direction/forward
top level: BBa_B1008
start: 1
end: 40
orientation: inline

PolyA
type: SequenceAnnotation
persistent identity: annotation1901008
display id: annotation1901008
version: 1
top level: BBa_B1008
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_B1008/annotation1901008/range1901008/1

https://synbiohub.org/public/igem/BBa_B1008/annotation1901008/range1901008/1
type: Range
persistent identity: range1901008
display id: range1901008
version: 1
direction: direction/forward
top level: BBa_B1008
start: 32
end: 36
orientation: inline

modified thr terminator
type: SequenceAnnotation
persistent identity: annotation1901006
display id: annotation1901006
version: 1
top level: BBa_B1008
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_B1008/annotation1901006/range1901006/1

https://synbiohub.org/public/igem/BBa_B1008/annotation1901006/range1901006/1
type: Range
persistent identity: range1901006
display id: range1901006
version: 1
direction: direction/forward
top level: BBa_B1008
start: 10
end: 31
orientation: inline

PolyA
type: SequenceAnnotation
persistent identity: annotation1901007
display id: annotation1901007
version: 1
top level: BBa_B1008
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_B1008/annotation1901007/range1901007/1

https://synbiohub.org/public/igem/BBa_B1008/annotation1901007/range1901007/1
type: Range
persistent identity: range1901007
display id: range1901007
version: 1
direction: direction/reverse
top level: BBa_B1008
start: 5
end: 9
orientation: inline

BBa_K180012
type: ComponentDefinition
persistent identity: BBa K180012
display id: BBa_K180012
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Rock - Primary plasmid (part 2) [tac pR, mCherry and LasI generator]
creator: Richard Wilson, Stephanie Chambers
created: 2009-10-18T11:00:00Z
modified: 2015-05-08T01:11:06Z
discontinued: false
dominant: false
experience: experience/None
group u list: _278_
m user id: 0
owner id: 4082
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Part of the iGEM2009 Team:Southampton rock-paper-scissors 'rock' primary plasmid.
mutable notes: Regulated expression of LasI (C0178) and mCherry (K180008) by tac pR (K180000), controlled by presence of lac/IPTG or trp in the cell.
mutable provenance: See relevant basic biobrick parts (K180000, B0034, K180008, B0015)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K180000
component: BBa_B0034
component: BBa_B0010
component: BBa_K180008
component: BBa_B0012
component: BBa_C0178
sequence annotation: BBa_K180008
sequence annotation: BBa_B0034
sequence annotation: BBa_K180000
sequence annotation: BBa_B0012
sequence annotation: BBa_C0178
sequence annotation: BBa_B0010
sequence: https://synbiohub.org/public/igem/BBa_K180012_sequence/1

BBa_K180000
type: Component
persistent identity: component2053924
display id: component2053924
version: 1
top level: BBa_K180012
access: public
definition: https://synbiohub.org/public/igem/BBa_K180000/1

BBa_B0034
type: Component
persistent identity: component2053930
display id: component2053930
version: 1
top level: BBa_K180012
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0010
type: Component
persistent identity: component2053933
display id: component2053933
version: 1
top level: BBa_K180012
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_K180008
type: Component
persistent identity: component2053928
display id: component2053928
version: 1
top level: BBa_K180012
access: public
definition: https://synbiohub.org/public/igem/BBa_K180008/1

BBa_B0034
type: Component
persistent identity: component2053926
display id: component2053926
version: 1
top level: BBa_K180012
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0012
type: Component
persistent identity: component2053935
display id: component2053935
version: 1
top level: BBa_K180012
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_C0178
type: Component
persistent identity: component2053932
display id: component2053932
version: 1
top level: BBa_K180012
access: public
definition: https://synbiohub.org/public/igem/BBa_C0178/1

BBa_K180008
type: SequenceAnnotation
persistent identity: annotation2053928
display id: annotation2053928
version: 1
top level: BBa_K180012
location: https://synbiohub.org/public/igem/BBa_K180012/annotation2053928/range2053928/1
component: BBa_K180008

https://synbiohub.org/public/igem/BBa_K180012/annotation2053928/range2053928/1
type: Range
persistent identity: range2053928
display id: range2053928
version: 1
direction: direction/reverse
top level: BBa_K180012
start: 1474
end: 2181
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2053926
display id: annotation2053926
version: 1
top level: BBa_K180012
location: https://synbiohub.org/public/igem/BBa_K180012/annotation2053926/range2053926/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K180012/annotation2053926/range2053926/1
type: Range
persistent identity: range2053926
display id: range2053926
version: 1
direction: direction/reverse
top level: BBa_K180012
start: 1456
end: 1467
orientation: inline

BBa_K180000
type: SequenceAnnotation
persistent identity: annotation2053924
display id: annotation2053924
version: 1
top level: BBa_K180012
location: https://synbiohub.org/public/igem/BBa_K180012/annotation2053924/range2053924/1
component: BBa_K180000

https://synbiohub.org/public/igem/BBa_K180012/annotation2053924/range2053924/1
type: Range
persistent identity: range2053924
display id: range2053924
version: 1
direction: direction/reverse
top level: BBa_K180012
start: 1
end: 1447
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2053935
display id: annotation2053935
version: 1
top level: BBa_K180012
location: https://synbiohub.org/public/igem/BBa_K180012/annotation2053935/range2053935/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K180012/annotation2053935/range2053935/1
type: Range
persistent identity: range2053935
display id: range2053935
version: 1
direction: direction/forward
top level: BBa_K180012
start: 2913
end: 2953
orientation: inline

BBa_C0178
type: SequenceAnnotation
persistent identity: annotation2053932
display id: annotation2053932
version: 1
top level: BBa_K180012
location: https://synbiohub.org/public/igem/BBa_K180012/annotation2053932/range2053932/1
component: BBa_C0178

https://synbiohub.org/public/igem/BBa_K180012/annotation2053932/range2053932/1
type: Range
persistent identity: range2053932
display id: range2053932
version: 1
direction: direction/reverse
top level: BBa_K180012
start: 2208
end: 2816
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2053933
display id: annotation2053933
version: 1
top level: BBa_K180012
location: https://synbiohub.org/public/igem/BBa_K180012/annotation2053933/range2053933/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K180012/annotation2053933/range2053933/1
type: Range
persistent identity: range2053933
display id: range2053933
version: 1
direction: direction/forward
top level: BBa_K180012
start: 2825
end: 2904
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2053930
display id: annotation2053930
version: 1
top level: BBa_K180012
location: https://synbiohub.org/public/igem/BBa_K180012/annotation2053930/range2053930/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K180012/annotation2053930/range2053930/1
type: Range
persistent identity: range2053930
display id: range2053930
version: 1
direction: direction/reverse
top level: BBa_K180012
start: 2190
end: 2201
orientation: inline

BBa_K113012
type: ComponentDefinition
persistent identity: BBa K113012
display id: BBa_K113012
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: weaken overlapping T7 promoter
creator: Wu Jingjing, Wang Jinyu
created: 2008-10-25T11:00:00Z
modified: 2015-05-08T01:09:20Z
discontinued: false
dominant: false
experience: experience/None
group u list: _254_
m user id: 0
owner id: 2711
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Two T7 promoters are reversely overlapping in two base pairs while a point mutation occurs at the 12th base pair, from A-T to T-A.
mutable notes: It is difficult to make this short DNA by PCR as both sides are T7 promoters, which are very similar. It is highly possible that they form loops before they can be synthesized by PCR. Thus we order two single stranded DNA and then anneal them together by touchdown PCR.
mutable provenance: The sequence of T7 promoter is TAATACGACTCACTATA (from 5' to 3').
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: T7 promoter
sequence: https://synbiohub.org/public/igem/BBa_K113012_sequence/1

T7 promoter
type: SequenceAnnotation
persistent identity: annotation1989402
display id: annotation1989402
version: 1
top level: BBa_K113012
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K113012/annotation1989402/range1989402/1

https://synbiohub.org/public/igem/BBa_K113012/annotation1989402/range1989402/1
type: Range
persistent identity: range1989402
display id: range1989402
version: 1
direction: direction/forward
top level: BBa_K113012
start: 20
end: 37
orientation: inline

T7 promoter
type: SequenceAnnotation
persistent identity: annotation1989400
display id: annotation1989400
version: 1
top level: BBa_K113012
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K113012/annotation1989400/range1989400/1

https://synbiohub.org/public/igem/BBa_K113012/annotation1989400/range1989400/1
type: Range
persistent identity: range1989400
display id: range1989400
version: 1
direction: direction/reverse
top level: BBa_K113012
start: 4
end: 21
orientation: inline

BBa_K259006
type: ComponentDefinition
persistent identity: BBa K259006
display id: BBa_K259006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: GFP-Terminator
creator: Petros Mina
created: 2009-09-08T11:00:00Z
modified: 2015-05-08T01:11:42Z
discontinued: false
dominant: false
group u list: _357_
m user id: 0
owner id: 5318
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: ==Part Description== This is a composite part made from Green Fluorescent Protein (GFP) and a double terminator. Green Fluorescent protein (originally from part [http://partsregistry.org/Part:BBa_E0040 BBa_E0040]) that will glow green when excited by the correct wavelengths and thus can be used as a reporter gene. This part is directly fused to a terminator (originally from part [http://partsregistry.org/Part:BBa_B0014 BBa_B0014]) so it is ideal to be fused at the end of your construct. ==Table of Statistics== {| style="color:white; background-color:#0066CC;" cellpadding="20" cellspacing="0" border="2" !Chassis !Monomer/Multimer !Aminoacid Length/Weight !Localisation !Structure !Excitation wavelengths !Emmision wavelengths |- |Prokaryotic and Eukaryotic |Bipartite |274 aminoacids | |beta-barrel(for GFP) hairpins for Terminator |501nm |511nm |} ==References== <biblio> #Valdivia pmid=8707053 </biblio>
mutable notes: No additional design considerations. The parts were fused together following RFC10 assembly.
mutable provenance: GFP originates from jellyfish Aequeora victoria. For more details visit the [http://www.expasy.ch/uniprot/P42212 SwissProt entry]. The double terminator was designed by Reshma Shetty. For more details visis the [http://partsregistry.org/Part:BBa_B0014 original part page].
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_B0011
component: BBa_E0040
component: BBa_B0012
sequence annotation: BBa_B0012
sequence annotation: BBa_E0040
sequence annotation: BBa_B0011
sequence: https://synbiohub.org/public/igem/BBa_K259006_sequence/1

BBa_B0011
type: Component
persistent identity: component2020534
display id: component2020534
version: 1
top level: BBa_K259006
access: public
definition: BBa_B0011

BBa_E0040
type: Component
persistent identity: component2020529
display id: component2020529
version: 1
top level: BBa_K259006
access: public
definition: https://synbiohub.org/public/igem/BBa_E0040/1

BBa_B0012
type: Component
persistent identity: component2020530
display id: component2020530
version: 1
top level: BBa_K259006
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2020530
display id: annotation2020530
version: 1
top level: BBa_K259006
location: https://synbiohub.org/public/igem/BBa_K259006/annotation2020530/range2020530/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K259006/annotation2020530/range2020530/1
type: Range
persistent identity: range2020530
display id: range2020530
version: 1
direction: direction/forward
top level: BBa_K259006
start: 729
end: 769
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation2020529
display id: annotation2020529
version: 1
top level: BBa_K259006
location: https://synbiohub.org/public/igem/BBa_K259006/annotation2020529/range2020529/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_K259006/annotation2020529/range2020529/1
type: Range
persistent identity: range2020529
display id: range2020529
version: 1
direction: direction/reverse
top level: BBa_K259006
start: 1
end: 720
orientation: inline

BBa_B0011
type: SequenceAnnotation
persistent identity: annotation2020534
display id: annotation2020534
version: 1
top level: BBa_K259006
location: https://synbiohub.org/public/igem/BBa_K259006/annotation2020534/range2020534/1
component: BBa_B0011

https://synbiohub.org/public/igem/BBa_K259006/annotation2020534/range2020534/1
type: Range
persistent identity: range2020534
display id: range2020534
version: 1
direction: direction/forward
top level: BBa_K259006
start: 778
end: 823
orientation: inline

P1
type: ComponentDefinition
persistent identity: BBa K100001
display id: BBa_K100001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Edited Xylose Regulated Bi-Directional Operator 1
creator: Garrett Tobin
created: 2008-08-17T11:00:00Z
modified: 2015-05-08T01:08:41Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _213_
m user id: 0
owner id: 1752
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part contains the operator region controlling transcription for the xyl operon. This regulatory region is dependent upon the binding of CRP-cAMP and XylR bound to xylose for activation. Upon binding, transcription is activated in both directions (xylFGH and xylAB facing). This part has a scrambled CRP binding site and and altered RNA ploymerase binding site that was designed to more resemble the consensus sequence slightly.
mutable notes: Both directions were kept in the sequence of the part even though it is intended to work in the right facing direction because of possible looping that may occur as a transcriptional control. The RNA polymerase binding site was altered to make binding stronger, attempting to allow activation when XylR-xylose alone is bound (the absence of CRP-cAMP).
mutable provenance: Taken from E. Coli genome bases 3728830-3729092, base pairs changed at RNA polymerase bining sites.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: RNA poly. Binding Site
sequence annotation: Scrambled CRP Binding Site
sequence: https://synbiohub.org/public/igem/BBa_K100001_sequence/1

RNA poly. Binding Site
type: SequenceAnnotation
persistent identity: annotation1972257
display id: annotation1972257
version: 1
top level: P1
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K100001/annotation1972257/range1972257/1

https://synbiohub.org/public/igem/BBa_K100001/annotation1972257/range1972257/1
type: Range
persistent identity: range1972257
display id: range1972257
version: 1
direction: direction/forward
top level: P1
start: 243
end: 248
orientation: inline

Scrambled CRP Binding Site
type: SequenceAnnotation
persistent identity: annotation1972256
display id: annotation1972256
version: 1
top level: P1
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K100001/annotation1972256/range1972256/1

https://synbiohub.org/public/igem/BBa_K100001/annotation1972256/range1972256/1
type: Range
persistent identity: range1972256
display id: range1972256
version: 1
direction: direction/forward
top level: P1
start: 88
end: 104
orientation: inline

RNA poly. Binding Site
type: SequenceAnnotation
persistent identity: annotation1972258
display id: annotation1972258
version: 1
top level: P1
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K100001/annotation1972258/range1972258/1

https://synbiohub.org/public/igem/BBa_K100001/annotation1972258/range1972258/1
type: Range
persistent identity: range1972258
display id: range1972258
version: 1
direction: direction/forward
top level: P1
start: 265
end: 270
orientation: inline

RNA poly. Binding Site
type: SequenceAnnotation
persistent identity: annotation1972254
display id: annotation1972254
version: 1
top level: P1
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K100001/annotation1972254/range1972254/1

https://synbiohub.org/public/igem/BBa_K100001/annotation1972254/range1972254/1
type: Range
persistent identity: range1972254
display id: range1972254
version: 1
direction: direction/reverse
top level: P1
start: 21
end: 26
orientation: inline

RNA poly. Binding Site
type: SequenceAnnotation
persistent identity: annotation1972255
display id: annotation1972255
version: 1
top level: P1
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K100001/annotation1972255/range1972255/1

https://synbiohub.org/public/igem/BBa_K100001/annotation1972255/range1972255/1
type: Range
persistent identity: range1972255
display id: range1972255
version: 1
direction: direction/reverse
top level: P1
start: 45
end: 50
orientation: inline

PN
type: ComponentDefinition
persistent identity: BBa K100000
display id: BBa_K100000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Natural Xylose Regulated Bi-Directional Operator
creator: Garrett Tobin
created: 2008-08-17T11:00:00Z
modified: 2015-05-08T01:08:41Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _213_
m user id: 0
owner id: 1752
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part contains the operator region controlling transcription for the xyl operon. This regulatory region is dependent upon the binding of CRP-cAMP and XylR bound to xylose for activation. Upon binding, transcription is activated in both directions (xylFGH and xylAB facing). This part has an unscrambled CRP binding site and and unaltered promoter region (unlike BBa_K100001 through BBa_K100003).
mutable notes: Both directions were kept in the sequence of the part even though it is intended to work in the right facing direction because of possible looping that may occur as a transcriptional control.
mutable provenance: Taken from E. Coli genome bases 3728830-3729092
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: RNA poly. binding site
sequence annotation: CRP binding site
sequence: https://synbiohub.org/public/igem/BBa_K100000_sequence/1

RNA poly. binding site
type: SequenceAnnotation
persistent identity: annotation1972196
display id: annotation1972196
version: 1
top level: PN
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K100000/annotation1972196/range1972196/1

https://synbiohub.org/public/igem/BBa_K100000/annotation1972196/range1972196/1
type: Range
persistent identity: range1972196
display id: range1972196
version: 1
direction: direction/forward
top level: PN
start: 45
end: 50
orientation: inline

RNA poly. binding site
type: SequenceAnnotation
persistent identity: annotation1972173
display id: annotation1972173
version: 1
top level: PN
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K100000/annotation1972173/range1972173/1

https://synbiohub.org/public/igem/BBa_K100000/annotation1972173/range1972173/1
type: Range
persistent identity: range1972173
display id: range1972173
version: 1
direction: direction/forward
top level: PN
start: 21
end: 26
orientation: inline

RNA poly. binding site
type: SequenceAnnotation
persistent identity: annotation1972263
display id: annotation1972263
version: 1
top level: PN
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K100000/annotation1972263/range1972263/1

https://synbiohub.org/public/igem/BBa_K100000/annotation1972263/range1972263/1
type: Range
persistent identity: range1972263
display id: range1972263
version: 1
direction: direction/forward
top level: PN
start: 243
end: 248
orientation: inline

CRP binding site
type: SequenceAnnotation
persistent identity: annotation1972261
display id: annotation1972261
version: 1
top level: PN
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K100000/annotation1972261/range1972261/1

https://synbiohub.org/public/igem/BBa_K100000/annotation1972261/range1972261/1
type: Range
persistent identity: range1972261
display id: range1972261
version: 1
direction: direction/forward
top level: PN
start: 90
end: 107
orientation: inline

RNA poly. binding site
type: SequenceAnnotation
persistent identity: annotation1972266
display id: annotation1972266
version: 1
top level: PN
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K100000/annotation1972266/range1972266/1

https://synbiohub.org/public/igem/BBa_K100000/annotation1972266/range1972266/1
type: Range
persistent identity: range1972266
display id: range1972266
version: 1
direction: direction/forward
top level: PN
start: 264
end: 269
orientation: inline

BBa_B0051
type: ComponentDefinition
persistent identity: BBa B0051
display id: BBa_B0051
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Terminator (yciA/tonA, +/-)
creator: Caitlin Conboy
created: 2004-01-29T12:00:00Z
modified: 2015-08-31T04:07:20Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Bi-directional Terminator from pSMART HCKAN. E.coli derived, genbank U24206 bases 2694-2727 between yciA and tonA genes (converging transcripts)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0051_sequence/1

stem_loop
type: SequenceAnnotation
persistent identity: annotation318600
display id: annotation318600
version: 1
top level: BBa_B0051
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_B0051/annotation318600/range318600/1

https://synbiohub.org/public/igem/BBa_B0051/annotation318600/range318600/1
type: Range
persistent identity: range318600
display id: range318600
version: 1
direction: direction/forward
top level: BBa_B0051
start: 11
end: 26
orientation: inline

BBa_T1004
type: ComponentDefinition
persistent identity: BBa T1004
display id: BBa_T1004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Test part
creator: Randy Rettberg
created: 2010-10-20T11:00:00Z
modified: 2015-09-03T11:55:57Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 4206
owner id: 25
owning group id: 397
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: test of adding a composite part
mutable notes: None
mutable provenance: None
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0001953
role: partType/Scar
component: BBa_T1001
component: BBa_B0015
component: BBa_F2620
component: BBa_T1002
sequence annotation: BBa_T1001
sequence annotation: BBa_B0015
sequence annotation: BBa_T1002
sequence annotation: BBa_F2620
sequence: https://synbiohub.org/public/igem/BBa_T1004_sequence/1

BBa_T1001
type: Component
persistent identity: component2377269
display id: component2377269
version: 1
top level: BBa_T1004
access: public
definition: https://synbiohub.org/public/igem/BBa_T1001/1

BBa_B0015
type: Component
persistent identity: component2377302
display id: component2377302
version: 1
top level: BBa_T1004
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_F2620
type: Component
persistent identity: component2377295
display id: component2377295
version: 1
top level: BBa_T1004
access: public
definition: https://synbiohub.org/public/igem/BBa_F2620/1

BBa_T1002
type: Component
persistent identity: component2377268
display id: component2377268
version: 1
top level: BBa_T1004
access: public
definition: https://synbiohub.org/public/igem/BBa_T1002/1

BBa_T1001
type: Component
persistent identity: component2377253
display id: component2377253
version: 1
top level: BBa_T1004
access: public
definition: https://synbiohub.org/public/igem/BBa_T1001/1

BBa_B0015
type: Component
persistent identity: component2377262
display id: component2377262
version: 1
top level: BBa_T1004
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_T1001
type: SequenceAnnotation
persistent identity: annotation2377269
display id: annotation2377269
version: 1
top level: BBa_T1004
location: https://synbiohub.org/public/igem/BBa_T1004/annotation2377269/range2377269/1
component: BBa_T1001

https://synbiohub.org/public/igem/BBa_T1004/annotation2377269/range2377269/1
type: Range
persistent identity: range2377269
display id: range2377269
version: 1
direction: direction/reverse
top level: BBa_T1004
start: 1027
end: 1078
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2377302
display id: annotation2377302
version: 1
top level: BBa_T1004
location: https://synbiohub.org/public/igem/BBa_T1004/annotation2377302/range2377302/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_T1004/annotation2377302/range2377302/1
type: Range
persistent identity: range2377302
display id: range2377302
version: 1
direction: direction/reverse
top level: BBa_T1004
start: 2156
end: 2284
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2377262
display id: annotation2377262
version: 1
top level: BBa_T1004
location: https://synbiohub.org/public/igem/BBa_T1004/annotation2377262/range2377262/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_T1004/annotation2377262/range2377262/1
type: Range
persistent identity: range2377262
display id: range2377262
version: 1
direction: direction/reverse
top level: BBa_T1004
start: 890
end: 1018
orientation: inline

BBa_T1002
type: SequenceAnnotation
persistent identity: annotation2377268
display id: annotation2377268
version: 1
top level: BBa_T1004
location: https://synbiohub.org/public/igem/BBa_T1004/annotation2377268/range2377268/1
component: BBa_T1002

https://synbiohub.org/public/igem/BBa_T1004/annotation2377268/range2377268/1
type: Range
persistent identity: range2377268
display id: range2377268
version: 1
direction: direction/reverse
top level: BBa_T1004
start: 61
end: 1018
orientation: inline

BBa_T1001
type: SequenceAnnotation
persistent identity: annotation2377253
display id: annotation2377253
version: 1
top level: BBa_T1004
location: https://synbiohub.org/public/igem/BBa_T1004/annotation2377253/range2377253/1
component: BBa_T1001

https://synbiohub.org/public/igem/BBa_T1004/annotation2377253/range2377253/1
type: Range
persistent identity: range2377253
display id: range2377253
version: 1
direction: direction/reverse
top level: BBa_T1004
start: 1
end: 52
orientation: inline

BBa_F2620
type: SequenceAnnotation
persistent identity: annotation2377295
display id: annotation2377295
version: 1
top level: BBa_T1004
location: https://synbiohub.org/public/igem/BBa_T1004/annotation2377295/range2377295/1
component: BBa_F2620

https://synbiohub.org/public/igem/BBa_T1004/annotation2377295/range2377295/1
type: Range
persistent identity: range2377295
display id: range2377295
version: 1
direction: direction/reverse
top level: BBa_T1004
start: 1087
end: 2147
orientation: inline