//direction/forward
type: Collection
persistent identity: direction forward
display id: direction_forward
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
owned by: james
owned by: myers
member: BBa_K110016
member: BBa_I51003
member: BBa_K1585103
member: https://synbiohub.org/public/igem/BBa_I732204/1
member: BBa_K256020
member: BBa_K136004
member: BBa_K1486046
member: U049O11+D0
member: BBa_J61118
member: BBa_K180000
member: BBa_B0053
member: U097NUL+D0
member: OmpR
member: BBa_K206001
member: BBa_J23108
member: BBa_K1486016
member: Mdaa
member: Pxyl
member: BBa_K1886017
member: BBa_K1897002
member: GFP-TS
member: pJEJAM11
member: BBa_K124000
member: pJEJAM16
member: mRFP1-NLS
member: BBa_K2042006
member: BBa_K863200
member: LasR+PAI
member: BBa_K086032
member: NOR2
member: BBa_M13502
member: BBa_K180018
member: BBa_I739106
member: pspoIIE
member: BBa_I13406
member: https://synbiohub.org/public/igem/BBa_K091157/1
member: Kozak & st
member: BBa_J23115
member: eGFP
member: BBa_I742153
member: tetA
member: BBa_I756014
member: BBa_K338081
member: BBa_J61123
member: BBa_K112402
member: https://synbiohub.org/public/igem/BBa_I719005/1
member: BBa_K086030
member: BBa_I746361
member: https://synbiohub.org/public/igem/BBa_J64981/1
member: BBa_J61125
member: https://synbiohub.org/public/igem/BBa_J64951/1
member: BBa_K105029
member: kanR
member: Pmra
member: BBa_I712004
member: nickname
member: https://synbiohub.org/public/igem/BBa_K1486018/1
member: BBa_K1486048
member: BBa_K1585110
member: BBa_I746363
member: BBa_K110009
member: BBa_K124002
member: pJEJAM18
member: TtrpC
member: BBa_B0030
member: P0310
member: BBa_K1486023
member: BBa_K165041
member: Pnot(O66)
member: BBa_K1486053

BBa_I739105
type: ComponentDefinition
persistent identity: BBa I739105
display id: BBa_I739105
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Double Promoter (LuxR/HSL, positive / cI, negative)
creator: Stefan Luzi
created: 2007-10-14T11:00:00Z
modified: 2015-08-31T04:08:01Z
discontinued: false
dominant: false
experience: experience/None
group u list: _124_
m user id: 0
owner id: 2183
owning group id: 62
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: asd
mutable notes: asdf
mutable provenance: Synthetic DNA
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: OR2
sequence annotation: R0062
sequence annotation: Scar
sequence annotation: R0051 (2x OR2)
sequence: https://synbiohub.org/public/igem/BBa_I739105_sequence/1

OR2
type: SequenceAnnotation
persistent identity: annotation1947917
display id: annotation1947917
version: 1
top level: BBa_I739105
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I739105/annotation1947917/range1947917/1

https://synbiohub.org/public/igem/BBa_I739105/annotation1947917/range1947917/1
type: Range
persistent identity: range1947917
display id: range1947917
version: 1
direction: direction/forward
top level: BBa_I739105
start: 82
end: 99
orientation: inline

R0062
type: SequenceAnnotation
persistent identity: annotation1947914
display id: annotation1947914
version: 1
top level: BBa_I739105
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I739105/annotation1947914/range1947914/1

https://synbiohub.org/public/igem/BBa_I739105/annotation1947914/range1947914/1
type: Range
persistent identity: range1947914
display id: range1947914
version: 1
direction: direction/forward
top level: BBa_I739105
start: 1
end: 55
orientation: inline

Scar
type: SequenceAnnotation
persistent identity: annotation1947915
display id: annotation1947915
version: 1
top level: BBa_I739105
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I739105/annotation1947915/range1947915/1

https://synbiohub.org/public/igem/BBa_I739105/annotation1947915/range1947915/1
type: Range
persistent identity: range1947915
display id: range1947915
version: 1
direction: direction/forward
top level: BBa_I739105
start: 56
end: 63
orientation: inline

OR2
type: SequenceAnnotation
persistent identity: annotation1947916
display id: annotation1947916
version: 1
top level: BBa_I739105
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I739105/annotation1947916/range1947916/1

https://synbiohub.org/public/igem/BBa_I739105/annotation1947916/range1947916/1
type: Range
persistent identity: range1947916
display id: range1947916
version: 1
direction: direction/forward
top level: BBa_I739105
start: 64
end: 81
orientation: inline

R0051 (2x OR2)
type: SequenceAnnotation
persistent identity: annotation1947918
display id: annotation1947918
version: 1
top level: BBa_I739105
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_I739105/annotation1947918/range1947918/1

https://synbiohub.org/public/igem/BBa_I739105/annotation1947918/range1947918/1
type: Range
persistent identity: range1947918
display id: range1947918
version: 1
direction: direction/forward
top level: BBa_I739105
start: 64
end: 99
orientation: inline

Lux
type: ComponentDefinition
persistent identity: BBa I1051
display id: BBa_I1051
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Lux cassette right promoter
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:30Z
discontinued: false
dominant: true
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: The lux cassette of V. fischeri contains a left and a right promoter. The right promoter gives weak constitutive expression of downstream genes.This expression is up-regulated by the action of the Lux repressor, LuxR. Two molecules of LuxR protein form a complex with two molecules the signalling compound homoserine lactone (HSL). This complex binds to a palindromic site on the promoter, increasing the rate of transcription.In thisversion, a binding site for cI dimer has been added: binding to this site dominantly represses transcription.
mutable provenance: V. fishceri and phage lambda (courtesy of Ron Weiss)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: BBa_I1051
sequence annotation: cI (OR1)
sequence annotation: LuxR/HSL
sequence annotation: start
sequence annotation: -10
sequence annotation: -35
sequence: https://synbiohub.org/public/igem/BBa_I1051_sequence/1

BBa_I1051
type: SequenceAnnotation
persistent identity: annotation7059
display id: annotation7059
version: 1
top level: Lux
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_I1051/annotation7059/range7059/1

https://synbiohub.org/public/igem/BBa_I1051/annotation7059/range7059/1
type: Range
persistent identity: range7059
display id: range7059
version: 1
direction: direction/forward
top level: Lux
start: 1
end: 68
orientation: inline

cI (OR1)
type: SequenceAnnotation
persistent identity: annotation1939
display id: annotation1939
version: 1
top level: Lux
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I1051/annotation1939/range1939/1

https://synbiohub.org/public/igem/BBa_I1051/annotation1939/range1939/1
type: Range
persistent identity: range1939
display id: range1939
version: 1
direction: direction/forward
top level: Lux
start: 52
end: 68
orientation: inline

LuxR/HSL
type: SequenceAnnotation
persistent identity: annotation1936
display id: annotation1936
version: 1
top level: Lux
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I1051/annotation1936/range1936/1

https://synbiohub.org/public/igem/BBa_I1051/annotation1936/range1936/1
type: Range
persistent identity: range1936
display id: range1936
version: 1
direction: direction/forward
top level: Lux
start: 1
end: 20
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation1940
display id: annotation1940
version: 1
top level: Lux
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_I1051/annotation1940/range1940/1

https://synbiohub.org/public/igem/BBa_I1051/annotation1940/range1940/1
type: Range
persistent identity: range1940
display id: range1940
version: 1
direction: direction/forward
top level: Lux
start: 53
end: 53
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1938
display id: annotation1938
version: 1
top level: Lux
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I1051/annotation1938/range1938/1

https://synbiohub.org/public/igem/BBa_I1051/annotation1938/range1938/1
type: Range
persistent identity: range1938
display id: range1938
version: 1
direction: direction/forward
top level: Lux
start: 42
end: 47
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1937
display id: annotation1937
version: 1
top level: Lux
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I1051/annotation1937/range1937/1

https://synbiohub.org/public/igem/BBa_I1051/annotation1937/range1937/1
type: Range
persistent identity: range1937
display id: range1937
version: 1
direction: direction/forward
top level: Lux
start: 20
end: 25
orientation: inline

BBa_M13101
type: ComponentDefinition
persistent identity: BBa M13101
display id: BBa_M13101
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: M13K07 gene I promoter
creator: Natalie Kuldell
created: 2006-12-15T12:00:00Z
modified: 2015-05-08T01:13:55Z
discontinued: false
dominant: false
group u list: _45_
m user id: 0
owner id: 314
owning group id: 1
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This part is from the bacteriophage M13 genome, bp 3086-3132 in M13K07. It directs transcription of M13 gene VII (BBa_M13507, BBa_M13007). Its identity as a promoter was described in Gene (1980) 11:129-148 based on -10,-35 homology and fragment of genome able to bind RNAP.
mutable notes: none
mutable provenance: New England Biolabs M13K07. Sequenced in 2002 by L. Panganaban.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_M13101_sequence/1

ampR
type: ComponentDefinition
persistent identity: BBa P1002
display id: BBa_P1002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: ampicillin resistance cassette
creator: Reshma Shetty
created: 2006-01-23T12:00:00Z
modified: 2015-05-08T01:14:11Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _41_6_
m user id: 0
owner id: 126
owning group id: 45
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Ampicillin resistance cassette for modular BioBricks vector construction.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: ampicillin resistance
sequence annotation: ampR -35
sequence annotation: ampR -10
sequence annotation: ampR RBS
sequence: https://synbiohub.org/public/igem/BBa_P1002_sequence/1

ampicillin resistance
type: SequenceAnnotation
persistent identity: annotation1790786
display id: annotation1790786
version: 1
top level: ampR
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_P1002/annotation1790786/range1790786/1

https://synbiohub.org/public/igem/BBa_P1002/annotation1790786/range1790786/1
type: Range
persistent identity: range1790786
display id: range1790786
version: 1
direction: direction/forward
top level: ampR
start: 80
end: 943
orientation: inline

ampR -35
type: SequenceAnnotation
persistent identity: annotation1897109
display id: annotation1897109
version: 1
top level: ampR
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_P1002/annotation1897109/range1897109/1

https://synbiohub.org/public/igem/BBa_P1002/annotation1897109/range1897109/1
type: Range
persistent identity: range1897109
display id: range1897109
version: 1
direction: direction/forward
top level: ampR
start: 31
end: 36
orientation: inline

ampR -10
type: SequenceAnnotation
persistent identity: annotation1897110
display id: annotation1897110
version: 1
top level: ampR
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_P1002/annotation1897110/range1897110/1

https://synbiohub.org/public/igem/BBa_P1002/annotation1897110/range1897110/1
type: Range
persistent identity: range1897110
display id: range1897110
version: 1
direction: direction/forward
top level: ampR
start: 57
end: 62
orientation: inline

ampR RBS
type: SequenceAnnotation
persistent identity: annotation1897111
display id: annotation1897111
version: 1
top level: ampR
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_P1002/annotation1897111/range1897111/1

https://synbiohub.org/public/igem/BBa_P1002/annotation1897111/range1897111/1
type: Range
persistent identity: range1897111
display id: range1897111
version: 1
direction: direction/forward
top level: ampR
start: 71
end: 75
orientation: inline

BBa_M13507
type: ComponentDefinition
persistent identity: BBa M13507
display id: BBa_M13507
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: M13KO7 gene VII RBS
creator: Natalie Kuldell
created: 2006-12-14T12:00:00Z
modified: 2015-05-08T01:13:56Z
discontinued: false
dominant: true
experience: experience/None
group u list: _45_
m user id: 0
owner id: 314
owning group id: 1
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This RBS is part of the bacteriophage M13 genome, initiating translation of the gene VII message (BBa_M13007). The part aligns with base pairs 1092-1107 in M13K07 genome. It was identified as the RBS for gene VII message by Wezenbeek et al in Gene (1980) 11: 129-148
mutable notes: New England Biolabs M13K07. Sequenced in 2002 by L. Panganaban
mutable provenance: New England Biolabs M13K07. Sequenced in 2002 by L. Panganaban
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_M13507_sequence/1

mCherry-GO
type: ComponentDefinition
persistent identity: BBa K678013
display id: BBa_K678013
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: mCherry-GOI
creator: DTU-Danmark-2
created: 2011-09-12T11:00:00Z
modified: 2015-05-08T01:13:04Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _882_
m user id: 0
owner id: 10556
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part was designed for the Plug'n'Play with DNA assembly standard (RFC 80), therefore this part contains tails at the 5' and 3' ends as shown on the figure below.
mutable notes: No
mutable provenance: The Danish Cancer Society
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: Kozak
sequence annotation: mCherry-GOI
sequence annotation: Linker 2
sequence: https://synbiohub.org/public/igem/BBa_K678013_sequence/1

Kozak
type: SequenceAnnotation
persistent identity: annotation2127779
display id: annotation2127779
version: 1
top level: mCherry-GO
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K678013/annotation2127779/range2127779/1

https://synbiohub.org/public/igem/BBa_K678013/annotation2127779/range2127779/1
type: Range
persistent identity: range2127779
display id: range2127779
version: 1
direction: direction/forward
top level: mCherry-GO
start: 9
end: 19
orientation: inline

mCherry-GOI
type: SequenceAnnotation
persistent identity: annotation2127759
display id: annotation2127759
version: 1
top level: mCherry-GO
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K678013/annotation2127759/range2127759/1

https://synbiohub.org/public/igem/BBa_K678013/annotation2127759/range2127759/1
type: Range
persistent identity: range2127759
display id: range2127759
version: 1
direction: direction/forward
top level: mCherry-GO
start: 16
end: 723
orientation: inline

Linker 2
type: SequenceAnnotation
persistent identity: annotation2127758
display id: annotation2127758
version: 1
top level: mCherry-GO
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K678013/annotation2127758/range2127758/1

https://synbiohub.org/public/igem/BBa_K678013/annotation2127758/range2127758/1
type: Range
persistent identity: range2127758
display id: range2127758
version: 1
direction: direction/forward
top level: mCherry-GO
start: 1
end: 8
orientation: inline

Ph-s
type: ComponentDefinition
persistent identity: BBa K143015
display id: BBa_K143015
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter hyper-spank for B. subtilis
creator: Chris Hirst
created: 2008-09-17T11:00:00Z
modified: 2015-05-08T01:10:23Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _199_
m user id: 0
owner id: 3475
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Promoter hyper-spank is an inducible promoter that has been designed for high expression in ''B.subtilis''. Gene expression under the promoter hyper-spank can be induced by addition of Isopropyl β-D-1-thiogalactopyranoside (IPTG). The context with which we used the promoter hyper-spank, was to take an input of IPTG and give '''Polymerase Per Second'''(PoPS) as an output. IPTG does not induce the promoter hyper-spank directly, but requires the transcriptional regulator '''LacI''', ( BBa_K413035 ). This means that LacI must be constitutively expressed in ''B.subtilis'' in order to use the promoter hyper-spank.
mutable notes: Biobrick standard was applied to the promoter hyper-spank sequence.
mutable provenance: The part was designed using the sequence from the ''B.subtilis'' genome and from previously published papers 123. This sequence was then synthesised by Geneart.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: LacI Operator
sequence annotation: Sigma A -10
sequence annotation: Sigma A -35
sequence: https://synbiohub.org/public/igem/BBa_K143015_sequence/1

LacI Operator
type: SequenceAnnotation
persistent identity: annotation1976423
display id: annotation1976423
version: 1
top level: Ph-s
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K143015/annotation1976423/range1976423/1

https://synbiohub.org/public/igem/BBa_K143015/annotation1976423/range1976423/1
type: Range
persistent identity: range1976423
display id: range1976423
version: 1
direction: direction/forward
top level: Ph-s
start: 10
end: 30
orientation: inline

Sigma A -10
type: SequenceAnnotation
persistent identity: annotation1976425
display id: annotation1976425
version: 1
top level: Ph-s
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K143015/annotation1976425/range1976425/1

https://synbiohub.org/public/igem/BBa_K143015/annotation1976425/range1976425/1
type: Range
persistent identity: range1976425
display id: range1976425
version: 1
direction: direction/forward
top level: Ph-s
start: 69
end: 74
orientation: inline

LacI Operator
type: SequenceAnnotation
persistent identity: annotation1976426
display id: annotation1976426
version: 1
top level: Ph-s
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K143015/annotation1976426/range1976426/1

https://synbiohub.org/public/igem/BBa_K143015/annotation1976426/range1976426/1
type: Range
persistent identity: range1976426
display id: range1976426
version: 1
direction: direction/forward
top level: Ph-s
start: 81
end: 101
orientation: inline

Sigma A -35
type: SequenceAnnotation
persistent identity: annotation1976424
display id: annotation1976424
version: 1
top level: Ph-s
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K143015/annotation1976424/range1976424/1

https://synbiohub.org/public/igem/BBa_K143015/annotation1976424/range1976424/1
type: Range
persistent identity: range1976424
display id: range1976424
version: 1
direction: direction/forward
top level: Ph-s
start: 46
end: 50
orientation: inline

BBa_K091156
type: ComponentDefinition
persistent identity: BBa K091156
display id: BBa_K091156
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pLux
creator: Erin Feeney
created: 2008-07-24T11:00:00Z
modified: 2015-05-08T01:08:38Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _191_
m user id: 0
owner id: 3067
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: The pLux' promoter is a modified version of BBa_R0062 containing the -35 region TTTAAT in place of TTTACG. Transcriptional activation is expected to occur in the presence of LuxR and AI-1, just as with R0062. The modified -35 region was designed to serve as a control for the evaluation of BBa_K091156, the pLux+ Las- promoter.
mutable notes: This promoter is identical to pLux+ Las- with the exception of the Las box's absence
mutable provenance: This part was adapted from BBa_R0062.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -35 Region
sequence annotation: LuxR
sequence annotation: -10 Region
sequence: https://synbiohub.org/public/igem/BBa_K091156_sequence/1

-35 Region
type: SequenceAnnotation
persistent identity: annotation1968420
display id: annotation1968420
version: 1
top level: BBa_K091156
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K091156/annotation1968420/range1968420/1

https://synbiohub.org/public/igem/BBa_K091156/annotation1968420/range1968420/1
type: Range
persistent identity: range1968420
display id: range1968420
version: 1
direction: direction/forward
top level: BBa_K091156
start: 20
end: 25
orientation: inline

LuxR
type: SequenceAnnotation
persistent identity: annotation1968422
display id: annotation1968422
version: 1
top level: BBa_K091156
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K091156/annotation1968422/range1968422/1

https://synbiohub.org/public/igem/BBa_K091156/annotation1968422/range1968422/1
type: Range
persistent identity: range1968422
display id: range1968422
version: 1
direction: direction/forward
top level: BBa_K091156
start: 1
end: 20
orientation: inline

-10 Region
type: SequenceAnnotation
persistent identity: annotation1968421
display id: annotation1968421
version: 1
top level: BBa_K091156
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K091156/annotation1968421/range1968421/1

https://synbiohub.org/public/igem/BBa_K091156/annotation1968421/range1968421/1
type: Range
persistent identity: range1968421
display id: range1968421
version: 1
direction: direction/forward
top level: BBa_K091156
start: 42
end: 47
orientation: inline

Pnot(O17)
type: ComponentDefinition
persistent identity: BBa I732272
display id: BBa_I732272
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Family Member with Hybrid Operator (D001O17)
creator: Ma Rui
created: 2007-10-13T11:00:00Z
modified: 2015-08-31T04:07:57Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732272_sequence/1

tetA
type: ComponentDefinition
persistent identity: BBa P1005
display id: BBa_P1005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: tetracycline resistance cassette
creator: Reshma Shetty
created: 2006-02-03T12:00:00Z
modified: 2015-08-21T03:01:31Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _41_6_
m user id: 8248
owner id: 126
owning group id: 45
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This contains CmR, a terminator and then AmpR and a terminator.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: tetR promoter
sequence annotation: tetA
sequence: https://synbiohub.org/public/igem/BBa_P1005_sequence/1

tetR promoter
type: SequenceAnnotation
persistent identity: annotation1897140
display id: annotation1897140
version: 1
top level: tetA
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_P1005/annotation1897140/range1897140/1

https://synbiohub.org/public/igem/BBa_P1005/annotation1897140/range1897140/1
type: Range
persistent identity: range1897140
display id: range1897140
version: 1
direction: direction/forward
top level: tetA
start: 1
end: 89
orientation: inline

tetA
type: SequenceAnnotation
persistent identity: annotation1897141
display id: annotation1897141
version: 1
top level: tetA
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_P1005/annotation1897141/range1897141/1

https://synbiohub.org/public/igem/BBa_P1005/annotation1897141/range1897141/1
type: Range
persistent identity: range1897141
display id: range1897141
version: 1
direction: direction/forward
top level: tetA
start: 90
end: 1283
orientation: inline

GFP-PTS1
type: ComponentDefinition
persistent identity: BBa K678033
display id: BBa_K678033
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: GFP-PTS1
creator: DTU-Denmark-2
created: 2011-09-10T11:00:00Z
modified: 2015-05-08T01:13:04Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _882_
m user id: 0
owner id: 8341
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: GFP targeted to the peroxisomes of Aspergillus nidulans
mutable notes: Design considerations This part was designed for the Plug'n'Play with DNA assembly standard (RFC 80), therefore this part contains linkers at the 5' and 3' ends as shown on the figure below.
mutable provenance: to be announced
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: GFP
sequence annotation: PTS1
sequence annotation: stop
sequence annotation: Linker 2
sequence: https://synbiohub.org/public/igem/BBa_K678033_sequence/1

GFP
type: SequenceAnnotation
persistent identity: annotation2127396
display id: annotation2127396
version: 1
top level: GFP-PTS1
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K678033/annotation2127396/range2127396/1

https://synbiohub.org/public/igem/BBa_K678033/annotation2127396/range2127396/1
type: Range
persistent identity: range2127396
display id: range2127396
version: 1
direction: direction/forward
top level: GFP-PTS1
start: 9
end: 725
orientation: inline

PTS1
type: SequenceAnnotation
persistent identity: annotation2127397
display id: annotation2127397
version: 1
top level: GFP-PTS1
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K678033/annotation2127397/range2127397/1

https://synbiohub.org/public/igem/BBa_K678033/annotation2127397/range2127397/1
type: Range
persistent identity: range2127397
display id: range2127397
version: 1
direction: direction/forward
top level: GFP-PTS1
start: 726
end: 734
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2127398
display id: annotation2127398
version: 1
top level: GFP-PTS1
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K678033/annotation2127398/range2127398/1

https://synbiohub.org/public/igem/BBa_K678033/annotation2127398/range2127398/1
type: Range
persistent identity: range2127398
display id: range2127398
version: 1
direction: direction/forward
top level: GFP-PTS1
start: 735
end: 737
orientation: inline

Linker 2
type: SequenceAnnotation
persistent identity: annotation2127395
display id: annotation2127395
version: 1
top level: GFP-PTS1
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K678033/annotation2127395/range2127395/1

https://synbiohub.org/public/igem/BBa_K678033/annotation2127395/range2127395/1
type: Range
persistent identity: range2127395
display id: range2127395
version: 1
direction: direction/forward
top level: GFP-PTS1
start: 1
end: 8
orientation: inline

U097NUL+D0
type: ComponentDefinition
persistent identity: BBa I732411
display id: BBa_I732411
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Family Member (U097NUL+D050O22)
creator: Zhan Jian
created: 2007-09-22T11:00:00Z
modified: 2015-08-31T04:07:58Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1572
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: To be later edited if time permits.
mutable notes: To be later edited if time permits.
mutable provenance: To be later edited if time permits.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: +1
sequence annotation: O2
sequence annotation: consensus elements
sequence: https://synbiohub.org/public/igem/BBa_I732411_sequence/1

+1
type: SequenceAnnotation
persistent identity: annotation1944563
display id: annotation1944563
version: 1
top level: U097NUL+D0
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_I732411/annotation1944563/range1944563/1

https://synbiohub.org/public/igem/BBa_I732411/annotation1944563/range1944563/1
type: Range
persistent identity: range1944563
display id: range1944563
version: 1
direction: direction/forward
top level: U097NUL+D0
start: 137
end: 137
orientation: inline

O2
type: SequenceAnnotation
persistent identity: annotation1944562
display id: annotation1944562
version: 1
top level: U097NUL+D0
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_I732411/annotation1944562/range1944562/1

https://synbiohub.org/public/igem/BBa_I732411/annotation1944562/range1944562/1
type: Range
persistent identity: range1944562
display id: range1944562
version: 1
direction: direction/forward
top level: U097NUL+D0
start: 146
end: 165
orientation: inline

consensus elements
type: SequenceAnnotation
persistent identity: annotation1944561
display id: annotation1944561
version: 1
top level: U097NUL+D0
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I732411/annotation1944561/range1944561/1

https://synbiohub.org/public/igem/BBa_I732411/annotation1944561/range1944561/1
type: Range
persistent identity: range1944561
display id: range1944561
version: 1
direction: direction/forward
top level: U097NUL+D0
start: 61
end: 137
orientation: inline

Pnot(O61)
type: ComponentDefinition
persistent identity: BBa I732279
display id: BBa_I732279
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Family Member with Hybrid Operator (D001O61)
creator: Ma Rui
created: 2007-10-13T11:00:00Z
modified: 2015-08-31T04:07:57Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732279_sequence/1

BBa_I742145
type: ComponentDefinition
persistent identity: BBa I742145
display id: BBa_I742145
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: sam5 native rbs
creator: Chris French
created: 2007-10-23T11:00:00Z
modified: 2015-08-31T04:08:03Z
discontinued: false
dominant: false
experience: experience/None
group u list: _123_
m user id: 0
owner id: 837
owning group id: 163
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is the native ribosome binding site for sam5 (4-coumarate-3-hydroxylase) of the bacterium Saccharothrix espanaensis.
mutable notes: This is a virtual part to be added to coding sequence I742141.
mutable provenance: Saccharothrix espanaensis DSM 44229
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: rbs
sequence: https://synbiohub.org/public/igem/BBa_I742145_sequence/1

rbs
type: SequenceAnnotation
persistent identity: annotation1955475
display id: annotation1955475
version: 1
top level: BBa_I742145
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_I742145/annotation1955475/range1955475/1

https://synbiohub.org/public/igem/BBa_I742145/annotation1955475/range1955475/1
type: Range
persistent identity: range1955475
display id: range1955475
version: 1
direction: direction/forward
top level: BBa_I742145
start: 3
end: 9
orientation: inline

cI TP901
type: ComponentDefinition
persistent identity: BBa R0075
display id: BBa_R0075
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter (TP901 cI regulated)
creator: crackdots
created: 2004-01-27T12:00:00Z
modified: 2015-05-08T01:14:15Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: cI repressor from phage TP901 infecting L. lactis
mutable notes: operator sites: pR and pL
mutable provenance: TP901-1
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: cI tp901 OL
sequence annotation: cI tp901 OR
sequence annotation: -35
sequence annotation: -10
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_R0075_sequence/1

cI tp901 OL
type: SequenceAnnotation
persistent identity: annotation319984
display id: annotation319984
version: 1
top level: cI TP901
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0075/annotation319984/range319984/1

https://synbiohub.org/public/igem/BBa_R0075/annotation319984/range319984/1
type: Range
persistent identity: range319984
display id: range319984
version: 1
direction: direction/forward
top level: cI TP901
start: 85
end: 115
orientation: inline

cI tp901 OR
type: SequenceAnnotation
persistent identity: annotation302714
display id: annotation302714
version: 1
top level: cI TP901
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0075/annotation302714/range302714/1

https://synbiohub.org/public/igem/BBa_R0075/annotation302714/range302714/1
type: Range
persistent identity: range302714
display id: range302714
version: 1
direction: direction/forward
top level: cI TP901
start: 19
end: 57
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation319983
display id: annotation319983
version: 1
top level: cI TP901
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0075/annotation319983/range319983/1

https://synbiohub.org/public/igem/BBa_R0075/annotation319983/range319983/1
type: Range
persistent identity: range319983
display id: range319983
version: 1
direction: direction/forward
top level: cI TP901
start: 53
end: 59
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation319985
display id: annotation319985
version: 1
top level: cI TP901
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0075/annotation319985/range319985/1

https://synbiohub.org/public/igem/BBa_R0075/annotation319985/range319985/1
type: Range
persistent identity: range319985
display id: range319985
version: 1
direction: direction/forward
top level: cI TP901
start: 76
end: 82
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation320591
display id: annotation320591
version: 1
top level: cI TP901
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_R0075/annotation320591/range320591/1

https://synbiohub.org/public/igem/BBa_R0075/annotation320591/range320591/1
type: Range
persistent identity: range320591
display id: range320591
version: 1
direction: direction/forward
top level: cI TP901
start: 89
end: 89
orientation: inline

BBa_K165039
type: ComponentDefinition
persistent identity: BBa K165039
display id: BBa_K165039
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Zif268-HIV binding sites + ADH1 yeast promoter
creator: John Szymanski
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:10:56Z
discontinued: false
dominant: false
group u list: _267_
m user id: 0
owner id: 2512
owning group id: 58
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: The Zif268-HIV transcription factor binding sites on the ADH1 constitutive promoter. Transcription regulation should be constitutive plus regulated by Zif268-HIV-binding transcription factors.
mutable notes: -
mutable provenance: Constructed in our lab with parts from Caroline Ajo-Franklin and David Drubin.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
component: BBa_K165015
component: BBa_K165011
sequence annotation: BBa_K165015
sequence annotation: BBa_K165011
sequence: https://synbiohub.org/public/igem/BBa_K165039_sequence/1

BBa_K165015
type: Component
persistent identity: component1995517
display id: component1995517
version: 1
top level: BBa_K165039
access: public
definition: BBa_K165015

BBa_K165011
type: Component
persistent identity: component1995516
display id: component1995516
version: 1
top level: BBa_K165039
access: public
definition: https://synbiohub.org/public/igem/BBa_K165011/1

BBa_K165015
type: SequenceAnnotation
persistent identity: annotation1995517
display id: annotation1995517
version: 1
top level: BBa_K165039
location: https://synbiohub.org/public/igem/BBa_K165039/annotation1995517/range1995517/1
component: BBa_K165015

https://synbiohub.org/public/igem/BBa_K165039/annotation1995517/range1995517/1
type: Range
persistent identity: range1995517
display id: range1995517
version: 1
direction: direction/reverse
top level: BBa_K165039
start: 55
end: 1499
orientation: inline

BBa_K165011
type: SequenceAnnotation
persistent identity: annotation1995516
display id: annotation1995516
version: 1
top level: BBa_K165039
location: https://synbiohub.org/public/igem/BBa_K165039/annotation1995516/range1995516/1
component: BBa_K165011

https://synbiohub.org/public/igem/BBa_K165039/annotation1995516/range1995516/1
type: Range
persistent identity: range1995516
display id: range1995516
version: 1
direction: direction/reverse
top level: BBa_K165039
start: 1
end: 46
orientation: inline

BBa_M13105
type: ComponentDefinition
persistent identity: BBa M13105
display id: BBa_M13105
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: M13K07 gene V promoter
creator: Natalie Kuldell
created: 2006-12-15T12:00:00Z
modified: 2015-05-08T01:13:55Z
discontinued: false
dominant: false
group u list: _45_
m user id: 0
owner id: 314
owning group id: 1
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This part is from the bacteriophage M13 genome, bp 786-835 in M13K07. It directs transcription of M13 gene V (BBa_M13505, BBa_M13005). Its identity as a promoter was described in Gene (1980) 11:129-148 based on -10,-35 homology and fragment of genome able to bind RNAP.
mutable notes: none
mutable provenance: New England Biolabs M13K07. Sequenced in 2002 by L. Panganaban.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_M13105_sequence/1

luxR
type: ComponentDefinition
persistent identity: BBa R0061
display id: BBa_R0061
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter (HSL-mediated luxR repressor)
creator: Srini Devadas, David Gray, Ronny Krashinsky, Debra Lin, and Chris Zheng Liu
created: 2004-01-28T12:00:00Z
modified: 2015-05-08T01:14:15Z
discontinued: false
dominant: true
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This part involves the -10 binding site, the -35 binding site, and the twenty nucleotides between that constitute the lux box. With this part, LuxR functions as a acyl-homoserine lactone-dependent repressor. LuxR resonds to the HSL produced by LuxI, N-(3-oxohexanoyl)-HSL. The Lux box is positioned such that it partially overlaps the consensus -35 and -10 hexamers of an RNA polymerase binding site.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: Lux Box
sequence annotation: -35
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R0061_sequence/1

Lux Box
type: SequenceAnnotation
persistent identity: annotation305877
display id: annotation305877
version: 1
top level: luxR
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0061/annotation305877/range305877/1

https://synbiohub.org/public/igem/BBa_R0061/annotation305877/range305877/1
type: Range
persistent identity: range305877
display id: range305877
version: 1
direction: direction/forward
top level: luxR
start: 6
end: 25
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation305863
display id: annotation305863
version: 1
top level: luxR
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0061/annotation305863/range305863/1

https://synbiohub.org/public/igem/BBa_R0061/annotation305863/range305863/1
type: Range
persistent identity: range305863
display id: range305863
version: 1
direction: direction/forward
top level: luxR
start: 1
end: 6
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation305864
display id: annotation305864
version: 1
top level: luxR
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0061/annotation305864/range305864/1

https://synbiohub.org/public/igem/BBa_R0061/annotation305864/range305864/1
type: Range
persistent identity: range305864
display id: range305864
version: 1
direction: direction/forward
top level: luxR
start: 25
end: 30
orientation: inline

BBa_J61126
type: ComponentDefinition
persistent identity: BBa J61126
display id: BBa_J61126
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Ribosome Binding Site Family Member
creator: John Anderson
created: 2007-04-24T11:00:00Z
modified: 2015-08-31T01:59:49Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: false
mutable description: {{JCA_Arkin_RBSFamily}}
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J61126_sequence/1

BBa_K1585103
type: ComponentDefinition
persistent identity: BBa K1585103
display id: BBa_K1585103
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Anderson Promoter with lacI binding site
creator: Tobias Schwanemann
created: 2015-08-31T11:00:00Z
modified: 2015-09-01T03:08:18Z
discontinued: false
dominant: false
experience: experience/Fails
group u list: _2002_
m user id: 24474
owner id: 24474
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Promoters of the Anderson Promoter Collection with lacI binding site derived from [http://parts.igem.org/Part:BBa_R0010 R_0010] to make them [http://openwetware.org/wiki/IPTG IPTG] iducible.
mutable notes: We choosed lacI-binding-site of part BBa_R0010 because we assumed older parts would be more reliable. The part is shipped in backbone [http://parts.igem.org/Part:BBa_J61002 J61002] because the functionality is directly visible by adding IPTG. RFP ([http://parts.igem.org/Part:BBa_E1010 E1010]) should get expressed then.
mutable provenance: Assambly of [http://parts.igem.org/Part:BBa_J23103 J23103] and lacI-binding-site of [http://parts.igem.org/Part:BBa_R0010 R_0010] The "lacI binding site" of Part [http://parts.igem.org/Part:BBa_R0010 R_0010] differs from lacO from Part [http://parts.igem.org/Part:BBa_J33207 BBa_J33207].
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
component: BBa_K1585999
component: BBa_J23103
sequence annotation: BBa_K1585999
sequence annotation: BBa_J23103
sequence: https://synbiohub.org/public/igem/BBa_K1585103_sequence/1

BBa_K1585999
type: Component
persistent identity: component2441413
display id: component2441413
version: 1
top level: BBa_K1585103
access: public
definition: https://synbiohub.org/public/igem/BBa_K1585999/1

BBa_J23103
type: Component
persistent identity: component2441411
display id: component2441411
version: 1
top level: BBa_K1585103
access: public
definition: BBa_J23103

BBa_K1585999
type: SequenceAnnotation
persistent identity: annotation2441413
display id: annotation2441413
version: 1
top level: BBa_K1585103
location: https://synbiohub.org/public/igem/BBa_K1585103/annotation2441413/range2441413/1
component: BBa_K1585999

https://synbiohub.org/public/igem/BBa_K1585103/annotation2441413/range2441413/1
type: Range
persistent identity: range2441413
display id: range2441413
version: 1
direction: direction/reverse
top level: BBa_K1585103
start: 44
end: 78
orientation: inline

BBa_J23103
type: SequenceAnnotation
persistent identity: annotation2441411
display id: annotation2441411
version: 1
top level: BBa_K1585103
location: https://synbiohub.org/public/igem/BBa_K1585103/annotation2441411/range2441411/1
component: BBa_J23103

https://synbiohub.org/public/igem/BBa_K1585103/annotation2441411/range2441411/1
type: Range
persistent identity: range2441411
display id: range2441411
version: 1
direction: direction/reverse
top level: BBa_K1585103
start: 1
end: 35
orientation: inline

ble
type: ComponentDefinition
persistent identity: BBa K1033990
display id: BBa_K1033990
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Zeocin resistance cassette
creator: Erik Gullberg
created: 2013-09-02T11:00:00Z
modified: 2015-05-08T01:08:50Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1340_
m user id: 0
owner id: 10137
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: Zeocoin and bleomycin resistance cassette including promoter and coding sequence. It lacks a terminator.
mutable notes: Codon optimized for E coli by GenScript.
mutable provenance: Streptoalloteichus hindustanus, PDB: 1BYL
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: RBS
sequence annotation: ble
sequence annotation: Promoter
sequence: https://synbiohub.org/public/igem/BBa_K1033990_sequence/1

RBS
type: SequenceAnnotation
persistent identity: annotation2334638
display id: annotation2334638
version: 1
top level: ble
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1033990/annotation2334638/range2334638/1

https://synbiohub.org/public/igem/BBa_K1033990/annotation2334638/range2334638/1
type: Range
persistent identity: range2334638
display id: range2334638
version: 1
direction: direction/forward
top level: ble
start: 58
end: 64
orientation: inline

ble
type: SequenceAnnotation
persistent identity: annotation2334639
display id: annotation2334639
version: 1
top level: ble
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1033990/annotation2334639/range2334639/1

https://synbiohub.org/public/igem/BBa_K1033990/annotation2334639/range2334639/1
type: Range
persistent identity: range2334639
display id: range2334639
version: 1
direction: direction/forward
top level: ble
start: 72
end: 446
orientation: inline

Promoter
type: SequenceAnnotation
persistent identity: annotation2334637
display id: annotation2334637
version: 1
top level: ble
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1033990/annotation2334637/range2334637/1

https://synbiohub.org/public/igem/BBa_K1033990/annotation2334637/range2334637/1
type: Range
persistent identity: range2334637
display id: range2334637
version: 1
direction: direction/forward
top level: ble
start: 2
end: 45
orientation: inline

BBa_K1585106
type: ComponentDefinition
persistent identity: BBa K1585106
display id: BBa_K1585106
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Anderson Promoter with lacI binding site
creator: Tobias Schwanemann
created: 2015-08-31T11:00:00Z
modified: 2015-09-01T02:01:39Z
discontinued: false
dominant: false
experience: experience/Fails
group u list: _2002_
m user id: 24474
owner id: 24474
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Promoters of the Anderson Promoter Collection with lacI binding site derived from [http://parts.igem.org/Part:BBa_R0010 R_0010] to make them [http://openwetware.org/wiki/IPTG IPTG] iducible.
mutable notes: We choosed lacI-binding-site of part BBa_R0010 because we assumed older parts would be more reliable. The part is shipped in backbone [http://parts.igem.org/Part:BBa_J61002 J61002] because the functionality is directly visible by adding IPTG. RFP ([http://parts.igem.org/Part:BBa_E1010 E1010]) should get expressed then.
mutable provenance: Assambly of [http://parts.igem.org/Part:BBa_J23106 J23106] and lacI-binding-site of [http://parts.igem.org/Part:BBa_R0010 R_0010] The "lacI binding site" of Part [http://parts.igem.org/Part:BBa_R0010 R_0010] differs from lacO from Part [http://parts.igem.org/Part:BBa_J33207 BBa_J33207].
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
component: BBa_K1585999
component: BBa_J23106
sequence annotation: BBa_K1585999
sequence annotation: BBa_J23106
sequence: https://synbiohub.org/public/igem/BBa_K1585106_sequence/1

BBa_K1585999
type: Component
persistent identity: component2441698
display id: component2441698
version: 1
top level: BBa_K1585106
access: public
definition: https://synbiohub.org/public/igem/BBa_K1585999/1

BBa_J23106
type: Component
persistent identity: component2441696
display id: component2441696
version: 1
top level: BBa_K1585106
access: public
definition: BBa_J23106

BBa_K1585999
type: SequenceAnnotation
persistent identity: annotation2441698
display id: annotation2441698
version: 1
top level: BBa_K1585106
location: https://synbiohub.org/public/igem/BBa_K1585106/annotation2441698/range2441698/1
component: BBa_K1585999

https://synbiohub.org/public/igem/BBa_K1585106/annotation2441698/range2441698/1
type: Range
persistent identity: range2441698
display id: range2441698
version: 1
direction: direction/reverse
top level: BBa_K1585106
start: 44
end: 78
orientation: inline

BBa_J23106
type: SequenceAnnotation
persistent identity: annotation2441696
display id: annotation2441696
version: 1
top level: BBa_K1585106
location: https://synbiohub.org/public/igem/BBa_K1585106/annotation2441696/range2441696/1
component: BBa_J23106

https://synbiohub.org/public/igem/BBa_K1585106/annotation2441696/range2441696/1
type: Range
persistent identity: range2441696
display id: range2441696
version: 1
direction: direction/reverse
top level: BBa_K1585106
start: 1
end: 35
orientation: inline

P(Lac) IQ
type: ComponentDefinition
persistent identity: BBa I14032
display id: BBa_I14032
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: promoter P(Lac) IQ
creator: Vikram Vijayan, Allen Hsu, Lawrence Fomundam
created: 2004-08-03T11:00:00Z
modified: 2015-08-31T04:07:37Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _4_
m user id: 0
owner id: 171
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Constitutive Promoter, High Transcription
mutable provenance: Plasmid pMAL-p2X
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -35
sequence annotation: P(Lac) IQ
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_I14032_sequence/1

-35
type: SequenceAnnotation
persistent identity: annotation1028343
display id: annotation1028343
version: 1
top level: P(Lac) IQ
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I14032/annotation1028343/range1028343/1

https://synbiohub.org/public/igem/BBa_I14032/annotation1028343/range1028343/1
type: Range
persistent identity: range1028343
display id: range1028343
version: 1
direction: direction/forward
top level: P(Lac) IQ
start: 3
end: 8
orientation: inline

P(Lac) IQ
type: SequenceAnnotation
persistent identity: annotation1028342
display id: annotation1028342
version: 1
top level: P(Lac) IQ
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_I14032/annotation1028342/range1028342/1

https://synbiohub.org/public/igem/BBa_I14032/annotation1028342/range1028342/1
type: Range
persistent identity: range1028342
display id: range1028342
version: 1
direction: direction/forward
top level: P(Lac) IQ
start: 1
end: 37
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1028344
display id: annotation1028344
version: 1
top level: P(Lac) IQ
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I14032/annotation1028344/range1028344/1

https://synbiohub.org/public/igem/BBa_I14032/annotation1028344/range1028344/1
type: Range
persistent identity: range1028344
display id: range1028344
version: 1
direction: direction/forward
top level: P(Lac) IQ
start: 26
end: 31
orientation: inline

BBa_K125100
type: ComponentDefinition
persistent identity: BBa K125100
display id: BBa_K125100
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: nir promoter from Synechocystis sp. PCC6803
creator: Krystle Salazar and Grace Kwan
created: 2008-07-23T11:00:00Z
modified: 2015-05-08T01:09:44Z
discontinued: false
dominant: false
experience: experience/None
group u list: _
m user id: 0
owner id: 3229
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: The ''nir'' promoter is nitrate inducible.
mutable provenance: The ''nirA'' promoter originates from ''Synechocystis'' sp. PCC6803.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: Ntc-B binding motif
sequence annotation: -10 sequence
sequence annotation: Ntc-A binding motif
sequence annotation: nir promoter
sequence: https://synbiohub.org/public/igem/BBa_K125100_sequence/1

Ntc-B binding motif
type: SequenceAnnotation
persistent identity: annotation1968488
display id: annotation1968488
version: 1
top level: BBa_K125100
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K125100/annotation1968488/range1968488/1

https://synbiohub.org/public/igem/BBa_K125100/annotation1968488/range1968488/1
type: Range
persistent identity: range1968488
display id: range1968488
version: 1
direction: direction/forward
top level: BBa_K125100
start: 5
end: 20
orientation: inline

-10 sequence
type: SequenceAnnotation
persistent identity: annotation1968487
display id: annotation1968487
version: 1
top level: BBa_K125100
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K125100/annotation1968487/range1968487/1

https://synbiohub.org/public/igem/BBa_K125100/annotation1968487/range1968487/1
type: Range
persistent identity: range1968487
display id: range1968487
version: 1
direction: direction/forward
top level: BBa_K125100
start: 73
end: 78
orientation: inline

Ntc-A binding motif
type: SequenceAnnotation
persistent identity: annotation1968489
display id: annotation1968489
version: 1
top level: BBa_K125100
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K125100/annotation1968489/range1968489/1

https://synbiohub.org/public/igem/BBa_K125100/annotation1968489/range1968489/1
type: Range
persistent identity: range1968489
display id: range1968489
version: 1
direction: direction/forward
top level: BBa_K125100
start: 36
end: 44
orientation: inline

nir promoter
type: SequenceAnnotation
persistent identity: annotation1968486
display id: annotation1968486
version: 1
top level: BBa_K125100
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K125100/annotation1968486/range1968486/1

https://synbiohub.org/public/igem/BBa_K125100/annotation1968486/range1968486/1
type: Range
persistent identity: range1968486
display id: range1968486
version: 1
direction: direction/forward
top level: BBa_K125100
start: 1
end: 88
orientation: inline

TetR
type: ComponentDefinition
persistent identity: BBa P1001
display id: BBa_P1001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: tetracycline resistance cassette
creator: Austin Che
created: 2005-12-19T12:00:00Z
modified: 2015-05-08T01:14:11Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _41_
m user id: 0
owner id: 22
owning group id: 41
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This cassette provides the entire tetracycline resistance operon and will express TetR.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: tetA
sequence annotation: tetA promoter
sequence: https://synbiohub.org/public/igem/BBa_P1001_sequence/1

tetA
type: SequenceAnnotation
persistent identity: annotation1771321
display id: annotation1771321
version: 1
top level: TetR
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_P1001/annotation1771321/range1771321/1

https://synbiohub.org/public/igem/BBa_P1001/annotation1771321/range1771321/1
type: Range
persistent identity: range1771321
display id: range1771321
version: 1
direction: direction/forward
top level: TetR
start: 89
end: 1279
orientation: inline

tetA promoter
type: SequenceAnnotation
persistent identity: annotation1771322
display id: annotation1771322
version: 1
top level: TetR
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_P1001/annotation1771322/range1771322/1

https://synbiohub.org/public/igem/BBa_P1001/annotation1771322/range1771322/1
type: Range
persistent identity: range1771322
display id: range1771322
version: 1
direction: direction/forward
top level: TetR
start: 1
end: 88
orientation: inline

nickname
type: ComponentDefinition
persistent identity: BBa K1479004
display id: BBa_K1479004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Just for upload test
creator: ChenYB
created: 2014-09-30T11:00:00Z
modified: 2015-05-08T01:10:40Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1859_
m user id: 0
owner id: 21515
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This part is just for upload test. It is meaningless
mutable notes: NULL
mutable provenance: NULL
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: PolyA
sequence annotation: T7 TE
sequence annotation: modified thr terminator
sequence annotation: BBa_K1479001
sequence annotation: BBa_B0012
sequence annotation: polya
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence annotation: BBa_B0011
sequence annotation: stop
sequence annotation: B1007
sequence annotation: B1008
sequence annotation: 22
sequence annotation: BBa_B0014
sequence annotation: modified thr
sequence annotation: BBa_B0015
sequence annotation: misc
sequence annotation: BBa_B1008
sequence annotation: BBa_K1479002
sequence annotation: BBa_B1007
sequence: https://synbiohub.org/public/igem/BBa_K1479004_sequence/1

PolyA
type: SequenceAnnotation
persistent identity: annotation2397973
display id: annotation2397973
version: 1
top level: nickname
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_K1479004/annotation2397973/range2397973/1

https://synbiohub.org/public/igem/BBa_K1479004/annotation2397973/range2397973/1
type: Range
persistent identity: range2397973
display id: range2397973
version: 1
direction: direction/reverse
top level: nickname
start: 155
end: 160
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation2397978
display id: annotation2397978
version: 1
top level: nickname
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1479004/annotation2397978/range2397978/1

https://synbiohub.org/public/igem/BBa_K1479004/annotation2397978/range2397978/1
type: Range
persistent identity: range2397978
display id: range2397978
version: 1
direction: direction/forward
top level: nickname
start: 207
end: 226
orientation: inline

PolyA
type: SequenceAnnotation
persistent identity: annotation2397968
display id: annotation2397968
version: 1
top level: nickname
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_K1479004/annotation2397968/range2397968/1

https://synbiohub.org/public/igem/BBa_K1479004/annotation2397968/range2397968/1
type: Range
persistent identity: range2397968
display id: range2397968
version: 1
direction: direction/reverse
top level: nickname
start: 108
end: 112
orientation: inline

modified thr terminator
type: SequenceAnnotation
persistent identity: annotation2397969
display id: annotation2397969
version: 1
top level: nickname
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1479004/annotation2397969/range2397969/1

https://synbiohub.org/public/igem/BBa_K1479004/annotation2397969/range2397969/1
type: Range
persistent identity: range2397969
display id: range2397969
version: 1
direction: direction/forward
top level: nickname
start: 113
end: 134
orientation: inline

BBa_K1479001
type: SequenceAnnotation
persistent identity: annotation2397965
display id: annotation2397965
version: 1
top level: nickname
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1479004/annotation2397965/range2397965/1

https://synbiohub.org/public/igem/BBa_K1479004/annotation2397965/range2397965/1
type: Range
persistent identity: range2397965
display id: range2397965
version: 1
direction: direction/reverse
top level: nickname
start: 104
end: 431
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2397976
display id: annotation2397976
version: 1
top level: nickname
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1479004/annotation2397976/range2397976/1

https://synbiohub.org/public/igem/BBa_K1479004/annotation2397976/range2397976/1
type: Range
persistent identity: range2397976
display id: range2397976
version: 1
direction: direction/forward
top level: nickname
start: 200
end: 240
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation2397961
display id: annotation2397961
version: 1
top level: nickname
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_K1479004/annotation2397961/range2397961/1

https://synbiohub.org/public/igem/BBa_K1479004/annotation2397961/range2397961/1
type: Range
persistent identity: range2397961
display id: range2397961
version: 1
direction: direction/forward
top level: nickname
start: 28
end: 41
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2397983
display id: annotation2397983
version: 1
top level: nickname
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1479004/annotation2397983/range2397983/1

https://synbiohub.org/public/igem/BBa_K1479004/annotation2397983/range2397983/1
type: Range
persistent identity: range2397983
display id: range2397983
version: 1
direction: direction/forward
top level: nickname
start: 303
end: 382
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation2397982
display id: annotation2397982
version: 1
top level: nickname
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1479004/annotation2397982/range2397982/1

https://synbiohub.org/public/igem/BBa_K1479004/annotation2397982/range2397982/1
type: Range
persistent identity: range2397982
display id: range2397982
version: 1
direction: direction/forward
top level: nickname
start: 261
end: 283
orientation: inline

BBa_B0011
type: SequenceAnnotation
persistent identity: annotation2397963
display id: annotation2397963
version: 1
top level: nickname
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1479004/annotation2397963/range2397963/1

https://synbiohub.org/public/igem/BBa_K1479004/annotation2397963/range2397963/1
type: Range
persistent identity: range2397963
display id: range2397963
version: 1
direction: direction/forward
top level: nickname
start: 50
end: 95
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2397989
display id: annotation2397989
version: 1
top level: nickname
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1479004/annotation2397989/range2397989/1

https://synbiohub.org/public/igem/BBa_K1479004/annotation2397989/range2397989/1
type: Range
persistent identity: range2397989
display id: range2397989
version: 1
direction: direction/forward
top level: nickname
start: 424
end: 424
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation2397979
display id: annotation2397979
version: 1
top level: nickname
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_K1479004/annotation2397979/range2397979/1

https://synbiohub.org/public/igem/BBa_K1479004/annotation2397979/range2397979/1
type: Range
persistent identity: range2397979
display id: range2397979
version: 1
direction: direction/forward
top level: nickname
start: 227
end: 240
orientation: inline

B1007
type: SequenceAnnotation
persistent identity: annotation2397972
display id: annotation2397972
version: 1
top level: nickname
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1479004/annotation2397972/range2397972/1

https://synbiohub.org/public/igem/BBa_K1479004/annotation2397972/range2397972/1
type: Range
persistent identity: range2397972
display id: range2397972
version: 1
direction: direction/forward
top level: nickname
start: 152
end: 191
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation2397988
display id: annotation2397988
version: 1
top level: nickname
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_K1479004/annotation2397988/range2397988/1

https://synbiohub.org/public/igem/BBa_K1479004/annotation2397988/range2397988/1
type: Range
persistent identity: range2397988
display id: range2397988
version: 1
direction: direction/forward
top level: nickname
start: 418
end: 431
orientation: inline

B1008
type: SequenceAnnotation
persistent identity: annotation2397966
display id: annotation2397966
version: 1
top level: nickname
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1479004/annotation2397966/range2397966/1

https://synbiohub.org/public/igem/BBa_K1479004/annotation2397966/range2397966/1
type: Range
persistent identity: range2397966
display id: range2397966
version: 1
direction: direction/forward
top level: nickname
start: 104
end: 143
orientation: inline

22
type: SequenceAnnotation
persistent identity: annotation2397957
display id: annotation2397957
version: 1
top level: nickname
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1479004/annotation2397957/range2397957/1

https://synbiohub.org/public/igem/BBa_K1479004/annotation2397957/range2397957/1
type: Range
persistent identity: range2397957
display id: range2397957
version: 1
direction: direction/forward
top level: nickname
start: 1
end: 10
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation2397987
display id: annotation2397987
version: 1
top level: nickname
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1479004/annotation2397987/range2397987/1

https://synbiohub.org/public/igem/BBa_K1479004/annotation2397987/range2397987/1
type: Range
persistent identity: range2397987
display id: range2397987
version: 1
direction: direction/forward
top level: nickname
start: 398
end: 417
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2397980
display id: annotation2397980
version: 1
top level: nickname
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1479004/annotation2397980/range2397980/1

https://synbiohub.org/public/igem/BBa_K1479004/annotation2397980/range2397980/1
type: Range
persistent identity: range2397980
display id: range2397980
version: 1
direction: direction/forward
top level: nickname
start: 233
end: 233
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2397962
display id: annotation2397962
version: 1
top level: nickname
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1479004/annotation2397962/range2397962/1

https://synbiohub.org/public/igem/BBa_K1479004/annotation2397962/range2397962/1
type: Range
persistent identity: range2397962
display id: range2397962
version: 1
direction: direction/forward
top level: nickname
start: 34
end: 34
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation2397964
display id: annotation2397964
version: 1
top level: nickname
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1479004/annotation2397964/range2397964/1

https://synbiohub.org/public/igem/BBa_K1479004/annotation2397964/range2397964/1
type: Range
persistent identity: range2397964
display id: range2397964
version: 1
direction: direction/forward
top level: nickname
start: 62
end: 84
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2397959
display id: annotation2397959
version: 1
top level: nickname
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1479004/annotation2397959/range2397959/1

https://synbiohub.org/public/igem/BBa_K1479004/annotation2397959/range2397959/1
type: Range
persistent identity: range2397959
display id: range2397959
version: 1
direction: direction/forward
top level: nickname
start: 1
end: 41
orientation: inline

BBa_B0014
type: SequenceAnnotation
persistent identity: annotation2397977
display id: annotation2397977
version: 1
top level: nickname
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1479004/annotation2397977/range2397977/1

https://synbiohub.org/public/igem/BBa_K1479004/annotation2397977/range2397977/1
type: Range
persistent identity: range2397977
display id: range2397977
version: 1
direction: direction/reverse
top level: nickname
start: 200
end: 294
orientation: inline

modified thr
type: SequenceAnnotation
persistent identity: annotation2397974
display id: annotation2397974
version: 1
top level: nickname
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1479004/annotation2397974/range2397974/1

https://synbiohub.org/public/igem/BBa_K1479004/annotation2397974/range2397974/1
type: Range
persistent identity: range2397974
display id: range2397974
version: 1
direction: direction/forward
top level: nickname
start: 161
end: 182
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation2397960
display id: annotation2397960
version: 1
top level: nickname
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1479004/annotation2397960/range2397960/1

https://synbiohub.org/public/igem/BBa_K1479004/annotation2397960/range2397960/1
type: Range
persistent identity: range2397960
display id: range2397960
version: 1
direction: direction/forward
top level: nickname
start: 8
end: 27
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2397984
display id: annotation2397984
version: 1
top level: nickname
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1479004/annotation2397984/range2397984/1

https://synbiohub.org/public/igem/BBa_K1479004/annotation2397984/range2397984/1
type: Range
persistent identity: range2397984
display id: range2397984
version: 1
direction: direction/reverse
top level: nickname
start: 303
end: 431
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation2397985
display id: annotation2397985
version: 1
top level: nickname
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1479004/annotation2397985/range2397985/1

https://synbiohub.org/public/igem/BBa_K1479004/annotation2397985/range2397985/1
type: Range
persistent identity: range2397985
display id: range2397985
version: 1
direction: direction/forward
top level: nickname
start: 314
end: 357
orientation: inline

PolyA
type: SequenceAnnotation
persistent identity: annotation2397975
display id: annotation2397975
version: 1
top level: nickname
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_K1479004/annotation2397975/range2397975/1

https://synbiohub.org/public/igem/BBa_K1479004/annotation2397975/range2397975/1
type: Range
persistent identity: range2397975
display id: range2397975
version: 1
direction: direction/forward
top level: nickname
start: 183
end: 188
orientation: inline

misc
type: SequenceAnnotation
persistent identity: annotation2397990
display id: annotation2397990
version: 1
top level: nickname
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1479004/annotation2397990/range2397990/1

https://synbiohub.org/public/igem/BBa_K1479004/annotation2397990/range2397990/1
type: Range
persistent identity: range2397990
display id: range2397990
version: 1
direction: direction/reverse
top level: nickname
start: 1
end: 20
orientation: inline

PolyA
type: SequenceAnnotation
persistent identity: annotation2397970
display id: annotation2397970
version: 1
top level: nickname
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_K1479004/annotation2397970/range2397970/1

https://synbiohub.org/public/igem/BBa_K1479004/annotation2397970/range2397970/1
type: Range
persistent identity: range2397970
display id: range2397970
version: 1
direction: direction/forward
top level: nickname
start: 135
end: 139
orientation: inline

BBa_B1008
type: SequenceAnnotation
persistent identity: annotation2397967
display id: annotation2397967
version: 1
top level: nickname
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1479004/annotation2397967/range2397967/1

https://synbiohub.org/public/igem/BBa_K1479004/annotation2397967/range2397967/1
type: Range
persistent identity: range2397967
display id: range2397967
version: 1
direction: direction/reverse
top level: nickname
start: 104
end: 143
orientation: inline

BBa_K1479002
type: SequenceAnnotation
persistent identity: annotation2397955
display id: annotation2397955
version: 1
top level: nickname
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1479004/annotation2397955/range2397955/1

https://synbiohub.org/public/igem/BBa_K1479004/annotation2397955/range2397955/1
type: Range
persistent identity: range2397955
display id: range2397955
version: 1
direction: direction/reverse
top level: nickname
start: 1
end: 95
orientation: inline

misc
type: SequenceAnnotation
persistent identity: annotation2397956
display id: annotation2397956
version: 1
top level: nickname
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1479004/annotation2397956/range2397956/1

https://synbiohub.org/public/igem/BBa_K1479004/annotation2397956/range2397956/1
type: Range
persistent identity: range2397956
display id: range2397956
version: 1
direction: direction/forward
top level: nickname
start: 1
end: 20
orientation: inline

BBa_B1007
type: SequenceAnnotation
persistent identity: annotation2397971
display id: annotation2397971
version: 1
top level: nickname
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1479004/annotation2397971/range2397971/1

https://synbiohub.org/public/igem/BBa_K1479004/annotation2397971/range2397971/1
type: Range
persistent identity: range2397971
display id: range2397971
version: 1
direction: direction/reverse
top level: nickname
start: 152
end: 191
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2397986
display id: annotation2397986
version: 1
top level: nickname
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1479004/annotation2397986/range2397986/1

https://synbiohub.org/public/igem/BBa_K1479004/annotation2397986/range2397986/1
type: Range
persistent identity: range2397986
display id: range2397986
version: 1
direction: direction/forward
top level: nickname
start: 391
end: 431
orientation: inline

BBa_B0011
type: SequenceAnnotation
persistent identity: annotation2397981
display id: annotation2397981
version: 1
top level: nickname
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1479004/annotation2397981/range2397981/1

https://synbiohub.org/public/igem/BBa_K1479004/annotation2397981/range2397981/1
type: Range
persistent identity: range2397981
display id: range2397981
version: 1
direction: direction/forward
top level: nickname
start: 249
end: 294
orientation: inline

BBa_K122000
type: ComponentDefinition
persistent identity: BBa K122000
display id: BBa_K122000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pPGK1
creator: David Ouyang
created: 2008-10-20T11:00:00Z
modified: 2015-05-08T01:09:42Z
discontinued: false
dominant: false
experience: experience/None
group u list: _218_
m user id: 0
owner id: 3326
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This is the 1500 bp upstream of the yeast PGK1 coding region. This is a putative constitutive promoter.
mutable notes: Source: Saccharomyces cerevisiae genomic DNA.
mutable provenance: This part was obtained from yeast genomic DNA.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K122000_sequence/1

BBa_K137086
type: ComponentDefinition
persistent identity: BBa K137086
display id: BBa_K137086
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: optimized (TA) repeat constitutive promoter with 15 bp between -10 and -35 elements
creator: Allen Lin
created: 2008-08-20T11:00:00Z
modified: 2015-05-08T01:10:10Z
discontinued: false
dominant: false
experience: experience/None
group u list: _187_
m user id: 0
owner id: 3112
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: optimized (TA) repeat constitutive promoter with 15 bp between -10 and -35 elements
mutable notes: None
mutable provenance: PCR
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K137086_sequence/1

BBa_K086025
type: ComponentDefinition
persistent identity: BBa K086025
display id: BBa_K086025
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: modified Lutz-Bujard LacO promoter,with alternative sigma factor σ28
creator: Gairik Sachdeva
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:08:37Z
discontinued: false
dominant: false
group u list: _197_
m user id: 0
owner id: 2578
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: modified Lutz-Bujard LacO promoter,with alternative sigma factor σ28
mutable notes: cleaned
mutable provenance: Lac O and sigma 28 binding sites' conserved sequences
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: P28_4
sequence: https://synbiohub.org/public/igem/BBa_K086025_sequence/1

P28_4
type: SequenceAnnotation
persistent identity: annotation1995759
display id: annotation1995759
version: 1
top level: BBa_K086025
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K086025/annotation1995759/range1995759/1

https://synbiohub.org/public/igem/BBa_K086025/annotation1995759/range1995759/1
type: Range
persistent identity: range1995759
display id: range1995759
version: 1
direction: direction/forward
top level: BBa_K086025
start: 1
end: 55
orientation: inline

TetR-4C
type: ComponentDefinition
persistent identity: BBa I12212
display id: BBa_I12212
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TetR - TetR-4C heterodimer promoter (negative)
creator: rwald
created: 2004-08-05T11:00:00Z
modified: 2015-08-31T04:07:31Z
discontinued: false
dominant: false
experience: experience/None
group u list: _3_
m user id: 0
owner id: 120
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This promoter is repressed by a heterodimer of wild-type TetR and mutant TetR-4C. It differs from wild-type TetR promoter in that the +4 and -4 regions of its O2 binding site have been changed, and the -35 region of the reverse promoter (pA) has been destroyed.
mutable notes: The O2 and O1 mutations are all single point mutations, occuring at the first base which appears listed here.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -35
sequence annotation: O1
sequence annotation: O2 mutation
sequence annotation: O1 mutation
sequence annotation: -10
sequence annotation: TetR O2
sequence: https://synbiohub.org/public/igem/BBa_I12212_sequence/1

-35
type: SequenceAnnotation
persistent identity: annotation1031337
display id: annotation1031337
version: 1
top level: TetR-4C
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I12212/annotation1031337/range1031337/1

https://synbiohub.org/public/igem/BBa_I12212/annotation1031337/range1031337/1
type: Range
persistent identity: range1031337
display id: range1031337
version: 1
direction: direction/forward
top level: TetR-4C
start: 1
end: 6
orientation: inline

O1
type: SequenceAnnotation
persistent identity: annotation1031341
display id: annotation1031341
version: 1
top level: TetR-4C
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I12212/annotation1031341/range1031341/1

https://synbiohub.org/public/igem/BBa_I12212/annotation1031341/range1031341/1
type: Range
persistent identity: range1031341
display id: range1031341
version: 1
direction: direction/forward
top level: TetR-4C
start: 32
end: 50
orientation: inline

O2 mutation
type: SequenceAnnotation
persistent identity: annotation1057573
display id: annotation1057573
version: 1
top level: TetR-4C
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I12212/annotation1057573/range1057573/1

https://synbiohub.org/public/igem/BBa_I12212/annotation1057573/range1057573/1
type: Range
persistent identity: range1057573
display id: range1057573
version: 1
direction: direction/forward
top level: TetR-4C
start: 7
end: 7
orientation: inline

O1 mutation
type: SequenceAnnotation
persistent identity: annotation1057574
display id: annotation1057574
version: 1
top level: TetR-4C
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I12212/annotation1057574/range1057574/1

https://synbiohub.org/public/igem/BBa_I12212/annotation1057574/range1057574/1
type: Range
persistent identity: range1057574
display id: range1057574
version: 1
direction: direction/forward
top level: TetR-4C
start: 37
end: 37
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1031340
display id: annotation1031340
version: 1
top level: TetR-4C
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I12212/annotation1031340/range1031340/1

https://synbiohub.org/public/igem/BBa_I12212/annotation1031340/range1031340/1
type: Range
persistent identity: range1031340
display id: range1031340
version: 1
direction: direction/forward
top level: TetR-4C
start: 26
end: 30
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1031339
display id: annotation1031339
version: 1
top level: TetR-4C
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I12212/annotation1031339/range1031339/1

https://synbiohub.org/public/igem/BBa_I12212/annotation1031339/range1031339/1
type: Range
persistent identity: range1031339
display id: range1031339
version: 1
direction: direction/forward
top level: TetR-4C
start: 22
end: 27
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1031342
display id: annotation1031342
version: 1
top level: TetR-4C
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I12212/annotation1031342/range1031342/1

https://synbiohub.org/public/igem/BBa_I12212/annotation1031342/range1031342/1
type: Range
persistent identity: range1031342
display id: range1031342
version: 1
direction: direction/forward
top level: TetR-4C
start: 45
end: 50
orientation: inline

TetR O2
type: SequenceAnnotation
persistent identity: annotation1031338
display id: annotation1031338
version: 1
top level: TetR-4C
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I12212/annotation1031338/range1031338/1

https://synbiohub.org/public/igem/BBa_I12212/annotation1031338/range1031338/1
type: Range
persistent identity: range1031338
display id: range1031338
version: 1
direction: direction/forward
top level: TetR-4C
start: 2
end: 20
orientation: inline

BBa_K1486032
type: ComponentDefinition
persistent identity: BBa K1486032
display id: BBa_K1486032
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: sfGFP + ADH1 terminator + Ura3 cassette
creator: EPFL iGem team 2014
created: 2014-09-26T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: To edit
mutable notes: To edit
mutable provenance: To edit
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: 8 a.a linker
sequence annotation: Hog1 5' flanking region
sequence annotation: BBa_K1486031
sequence annotation: ADH1 terminator
sequence annotation: BBa_K1486023
sequence annotation: Hog1 3' flanking region
sequence: https://synbiohub.org/public/igem/BBa_K1486032_sequence/1

8 a.a linker
type: SequenceAnnotation
persistent identity: annotation2417800
display id: annotation2417800
version: 1
top level: BBa_K1486032
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1486032/annotation2417800/range2417800/1

https://synbiohub.org/public/igem/BBa_K1486032/annotation2417800/range2417800/1
type: Range
persistent identity: range2417800
display id: range2417800
version: 1
direction: direction/forward
top level: BBa_K1486032
start: 48
end: 71
orientation: inline

Hog1 5' flanking region
type: SequenceAnnotation
persistent identity: annotation2417799
display id: annotation2417799
version: 1
top level: BBa_K1486032
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1486032/annotation2417799/range2417799/1

https://synbiohub.org/public/igem/BBa_K1486032/annotation2417799/range2417799/1
type: Range
persistent identity: range2417799
display id: range2417799
version: 1
direction: direction/forward
top level: BBa_K1486032
start: 1
end: 47
orientation: inline

BBa_K1486031
type: SequenceAnnotation
persistent identity: annotation2417804
display id: annotation2417804
version: 1
top level: BBa_K1486032
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1486032/annotation2417804/range2417804/1

https://synbiohub.org/public/igem/BBa_K1486032/annotation2417804/range2417804/1
type: Range
persistent identity: range2417804
display id: range2417804
version: 1
direction: direction/forward
top level: BBa_K1486032
start: 1070
end: 2430
orientation: inline

ADH1 terminator
type: SequenceAnnotation
persistent identity: annotation2417803
display id: annotation2417803
version: 1
top level: BBa_K1486032
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1486032/annotation2417803/range2417803/1

https://synbiohub.org/public/igem/BBa_K1486032/annotation2417803/range2417803/1
type: Range
persistent identity: range2417803
display id: range2417803
version: 1
direction: direction/forward
top level: BBa_K1486032
start: 808
end: 996
orientation: inline

BBa_K1486023
type: SequenceAnnotation
persistent identity: annotation2417801
display id: annotation2417801
version: 1
top level: BBa_K1486032
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1486032/annotation2417801/range2417801/1

https://synbiohub.org/public/igem/BBa_K1486032/annotation2417801/range2417801/1
type: Range
persistent identity: range2417801
display id: range2417801
version: 1
direction: direction/forward
top level: BBa_K1486032
start: 72
end: 804
orientation: inline

Hog1 3' flanking region
type: SequenceAnnotation
persistent identity: annotation2417805
display id: annotation2417805
version: 1
top level: BBa_K1486032
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1486032/annotation2417805/range2417805/1

https://synbiohub.org/public/igem/BBa_K1486032/annotation2417805/range2417805/1
type: Range
persistent identity: range2417805
display id: range2417805
version: 1
direction: direction/forward
top level: BBa_K1486032
start: 2494
end: 2544
orientation: inline

BBa_K165033
type: ComponentDefinition
persistent identity: BBa K165033
display id: BBa_K165033
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: YY1 binding sites + mCYC promoter
creator: John Szymanski
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:10:56Z
discontinued: false
dominant: false
group u list: _267_
m user id: 0
owner id: 2512
owning group id: 58
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: The YY1 transcription factor binding sites on the mCYC minimal promoter. All transcription regulation comes from YY1-binding transcription factors.
mutable notes: -
mutable provenance: Made in our lab with parts from Caroline Ajo-Franklin and David Drubin.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
component: BBa_K165013
component: BBa_K165016
sequence annotation: BBa_K165013
sequence annotation: BBa_K165016
sequence: https://synbiohub.org/public/igem/BBa_K165033_sequence/1

BBa_K165013
type: Component
persistent identity: component1995402
display id: component1995402
version: 1
top level: BBa_K165033
access: public
definition: https://synbiohub.org/public/igem/BBa_K165013/1

BBa_K165016
type: Component
persistent identity: component1995403
display id: component1995403
version: 1
top level: BBa_K165033
access: public
definition: BBa_K165016

BBa_K165013
type: SequenceAnnotation
persistent identity: annotation1995402
display id: annotation1995402
version: 1
top level: BBa_K165033
location: https://synbiohub.org/public/igem/BBa_K165033/annotation1995402/range1995402/1
component: BBa_K165013

https://synbiohub.org/public/igem/BBa_K165033/annotation1995402/range1995402/1
type: Range
persistent identity: range1995402
display id: range1995402
version: 1
direction: direction/reverse
top level: BBa_K165033
start: 1
end: 51
orientation: inline

BBa_K165016
type: SequenceAnnotation
persistent identity: annotation1995403
display id: annotation1995403
version: 1
top level: BBa_K165033
location: https://synbiohub.org/public/igem/BBa_K165033/annotation1995403/range1995403/1
component: BBa_K165016

https://synbiohub.org/public/igem/BBa_K165033/annotation1995403/range1995403/1
type: Range
persistent identity: range1995403
display id: range1995403
version: 1
direction: direction/reverse
top level: BBa_K165033
start: 60
end: 304
orientation: inline

U073O11+D0
type: ComponentDefinition
persistent identity: BBa I732430
display id: BBa_I732430
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Family Member (U073O11+D062O22)
creator: Zhan Jian
created: 2007-10-13T11:00:00Z
modified: 2015-08-31T04:07:58Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732430_sequence/1

U049O11+D0
type: ComponentDefinition
persistent identity: BBa I732437
display id: BBa_I732437
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Family Member (U049O11+D002O22)
creator: Zhan Jian
created: 2007-10-13T11:00:00Z
modified: 2015-08-31T04:07:58Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732437_sequence/1

BBa_K112900
type: ComponentDefinition
persistent identity: BBa K112900
display id: BBa_K112900
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Pbad
creator: Bing Xia
created: 2008-10-20T11:00:00Z
modified: 2015-05-08T01:09:19Z
discontinued: false
dominant: false
group u list: _224_
m user id: 0
owner id: 2998
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Arabinose induced promoter. ---- This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at: [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K112900_sequence/1

FadA
type: ComponentDefinition
persistent identity: BBa K861400
display id: BBa_K861400
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: FadA protein coding sequence for fatty acid degradation
creator: Kuanwei Sheng
created: 2012-08-15T11:00:00Z
modified: 2015-05-08T01:13:36Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1121_
m user id: 0
owner id: 12352
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: For a E.coli to degrade fatty acid, at least four enzymes ??? FadA, FadB, FadD, FadE and transporter???FadL are needed. Long chain fatty acids are firstly being imported by the transmembrane protein FadL. After FAs get into cells, they will be added a CoA by inner membrane-associated FadD (acyl-CoA synthase). β-oxidation is initiated by FadE(acyl-CoA dehydrogenase), which will convert acyl-CoA into enoyl-CoA. The followed cycles of hydration, oxidation, and thiolytic cleavage are carried out by tetrameric complex consisting of two FadA and two FadB monomers. This part is FadA coding sequence, which has 3-ketoacyl-CoA thiolase activity involving in the β-oxidation of living E.coli. The protein takes a part in the planning system to improve whole efficiency of fatty acid degradation. With appropriate promoters we decided, FadA expression can be under regulation of fatty acid concentration.
mutable notes: Before constructing the final system and testing its function, we constructed three biobrick parts with 3-ketoacyl-CoA thiolase activity (FadA, FadI, S-FadA) as candidates.
mutable provenance: Escherichia coli K.12 MG1655 genome
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: FadA coding sequence
sequence: https://synbiohub.org/public/igem/BBa_K861400_sequence/1

FadA coding sequence
type: SequenceAnnotation
persistent identity: annotation2179595
display id: annotation2179595
version: 1
top level: FadA
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K861400/annotation2179595/range2179595/1

https://synbiohub.org/public/igem/BBa_K861400/annotation2179595/range2179595/1
type: Range
persistent identity: range2179595
display id: range2179595
version: 1
direction: direction/forward
top level: FadA
start: 1
end: 1164
orientation: inline

BBa_K284002
type: ComponentDefinition
persistent identity: BBa K284002
display id: BBa_K284002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: JEN1 Promoter from Kluyveromyces lactis
creator: Raissa Estrela Curado, Tais Sineiro Herig and Gleidson Silva Teixeira
created: 2009-09-25T11:00:00Z
modified: 2015-05-08T01:11:48Z
discontinued: false
dominant: false
experience: experience/None
group u list: _386_
m user id: 0
owner id: 4968
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: .
mutable notes: .
mutable provenance: .
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: primer_binding
sequence annotation: TATA-Like
sequence annotation: JEN1 Promoter
sequence: https://synbiohub.org/public/igem/BBa_K284002_sequence/1

primer_binding
type: SequenceAnnotation
persistent identity: annotation2034979
display id: annotation2034979
version: 1
top level: BBa_K284002
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K284002/annotation2034979/range2034979/1

https://synbiohub.org/public/igem/BBa_K284002/annotation2034979/range2034979/1
type: Range
persistent identity: range2034979
display id: range2034979
version: 1
direction: direction/reverse
top level: BBa_K284002
start: 976
end: 998
orientation: inline

primer_binding
type: SequenceAnnotation
persistent identity: annotation2034977
display id: annotation2034977
version: 1
top level: BBa_K284002
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K284002/annotation2034977/range2034977/1

https://synbiohub.org/public/igem/BBa_K284002/annotation2034977/range2034977/1
type: Range
persistent identity: range2034977
display id: range2034977
version: 1
direction: direction/forward
top level: BBa_K284002
start: 1
end: 19
orientation: inline

TATA-Like
type: SequenceAnnotation
persistent identity: annotation2040986
display id: annotation2040986
version: 1
top level: BBa_K284002
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K284002/annotation2040986/range2040986/1

https://synbiohub.org/public/igem/BBa_K284002/annotation2040986/range2040986/1
type: Range
persistent identity: range2040986
display id: range2040986
version: 1
direction: direction/forward
top level: BBa_K284002
start: 862
end: 873
orientation: inline

JEN1 Promoter
type: SequenceAnnotation
persistent identity: annotation2034978
display id: annotation2034978
version: 1
top level: BBa_K284002
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K284002/annotation2034978/range2034978/1

https://synbiohub.org/public/igem/BBa_K284002/annotation2034978/range2034978/1
type: Range
persistent identity: range2034978
display id: range2034978
version: 1
direction: direction/forward
top level: BBa_K284002
start: 1
end: 998
orientation: inline

BBa_J56015
type: ComponentDefinition
persistent identity: BBa J56015
display id: BBa_J56015
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: lacIQ - promoter sequence
creator: Matt Eames
created: 2006-10-31T12:00:00Z
modified: 2015-08-31T03:22:59Z
discontinued: false
dominant: false
group u list: _79_98_
m user id: 0
owner id: 1132
owning group id: 98
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Promoter sequence for constituitive expression
mutable notes: None
mutable provenance: Synthesized by DNA 2.0
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J56015_sequence/1

Pspac-hy
type: ComponentDefinition
persistent identity: BBa I746665
display id: BBa_I746665
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Pspac-hy promoter
creator: Yue Miao
created: 2007-10-23T11:00:00Z
modified: 2015-08-31T04:08:05Z
discontinued: false
dominant: false
experience: experience/None
group u list: _116_
m user id: 0
owner id: 2119
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: There is a G->T point mutation of pSpac at the -1 site, changing the promoter to a stronger one - hence the name pSpac-hy, hy meaning hyper. This promoter drives transcription at rates of about ten times that of the wild type (pSpac) promoter.
mutable notes: NA
mutable provenance: Comes from the pPL82 vector, which is intended as a B. subtilis integrational vector. It is placed in front of the MCS in the plasmid, as it would drive the expression of the gene cloned into the MCS both in B. subtilis and E. coli.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I746665_sequence/1

BBa_K165038
type: ComponentDefinition
persistent identity: BBa K165038
display id: BBa_K165038
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Gli1 binding sites + ADH1 constitutive yeast promoter
creator: John Szymanski
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:10:56Z
discontinued: false
dominant: false
group u list: _267_
m user id: 0
owner id: 2512
owning group id: 58
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: The Gli1 transcription factor binding sites on the ADH1 constitutive promoter. Transcription regulation should be constitutive plus regulated by YY1-binding transcription factors.
mutable notes: -
mutable provenance: Constructed in our lab with parts from Caroline Ajo-Franklin and David Drubin.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
component: BBa_K165012
component: BBa_K165015
sequence annotation: BBa_K165015
sequence annotation: BBa_K165012
sequence: https://synbiohub.org/public/igem/BBa_K165038_sequence/1

BBa_K165012
type: Component
persistent identity: component1995512
display id: component1995512
version: 1
top level: BBa_K165038
access: public
definition: https://synbiohub.org/public/igem/BBa_K165012/1

BBa_K165015
type: Component
persistent identity: component1995513
display id: component1995513
version: 1
top level: BBa_K165038
access: public
definition: BBa_K165015

BBa_K165015
type: SequenceAnnotation
persistent identity: annotation1995513
display id: annotation1995513
version: 1
top level: BBa_K165038
location: https://synbiohub.org/public/igem/BBa_K165038/annotation1995513/range1995513/1
component: BBa_K165015

https://synbiohub.org/public/igem/BBa_K165038/annotation1995513/range1995513/1
type: Range
persistent identity: range1995513
display id: range1995513
version: 1
direction: direction/reverse
top level: BBa_K165038
start: 136
end: 1580
orientation: inline

BBa_K165012
type: SequenceAnnotation
persistent identity: annotation1995512
display id: annotation1995512
version: 1
top level: BBa_K165038
location: https://synbiohub.org/public/igem/BBa_K165038/annotation1995512/range1995512/1
component: BBa_K165012

https://synbiohub.org/public/igem/BBa_K165038/annotation1995512/range1995512/1
type: Range
persistent identity: range1995512
display id: range1995512
version: 1
direction: direction/reverse
top level: BBa_K165038
start: 1
end: 127
orientation: inline

lux pL
type: ComponentDefinition
persistent identity: BBa R0063
display id: BBa_R0063
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter (luxR & HSL regulated -- lux pL)
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:15Z
discontinued: false
dominant: true
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: The lux cassette of V. fischeri contains a left and a right promoter. The left promoter gives weak constitutive expression of downstream genes.This expression is down-regulated by the action of the Lux repressor, LuxR. Two molecules of LuxR protein form a complex with two molecules the signalling compound homoserine lactone (HSL). This complex binds to a palindromic site on the promoter, increasing the rate of transcription from Pr, repressing transcription from Pl
mutable notes: This promoter is based on the Vibrio fischeri quorum sensing gene promoters. Two genes LuxI and LuxR and transcribed in opposite directions as shown below. The original sequence from which the parts <bb_part>BBa_R0062</bb_part> and <bb_part>BBa_R0063</bb_part> were derived is shown in the picture below.Includes most of Lux reulatory region, including the LuxR binding site which activates the right promoter. A putative LuxR autorepression binding site is also identified adjacent to the -10 site of the right promoter. This 2nd site has 55% identity with the first site. Putative inverted repeats (of size 18-27 bp) also exist between these two sites (not marked above), which may represent binding sites for other regulatory proteins. <img src="<bb_file>Image01.gif</bb_file>" width="614" height="362"> Unspecified.
mutable provenance: V. fischeri.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: LuxR/HSL
sequence annotation: start
sequence annotation: BBa_R0063
sequence annotation: -35
sequence annotation: -10
sequence annotation: Putative LuxR/HSL
sequence: https://synbiohub.org/public/igem/BBa_R0063_sequence/1

LuxR/HSL
type: SequenceAnnotation
persistent identity: annotation2051
display id: annotation2051
version: 1
top level: lux pL
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0063/annotation2051/range2051/1

https://synbiohub.org/public/igem/BBa_R0063/annotation2051/range2051/1
type: Range
persistent identity: range2051
display id: range2051
version: 1
direction: direction/forward
top level: lux pL
start: 1
end: 20
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2054
display id: annotation2054
version: 1
top level: lux pL
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_R0063/annotation2054/range2054/1

https://synbiohub.org/public/igem/BBa_R0063/annotation2054/range2054/1
type: Range
persistent identity: range2054
display id: range2054
version: 1
direction: direction/forward
top level: lux pL
start: 128
end: 128
orientation: inline

BBa_R0063
type: SequenceAnnotation
persistent identity: annotation7071
display id: annotation7071
version: 1
top level: lux pL
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_R0063/annotation7071/range7071/1

https://synbiohub.org/public/igem/BBa_R0063/annotation7071/range7071/1
type: Range
persistent identity: range7071
display id: range7071
version: 1
direction: direction/forward
top level: lux pL
start: 1
end: 151
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2053
display id: annotation2053
version: 1
top level: lux pL
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0063/annotation2053/range2053/1

https://synbiohub.org/public/igem/BBa_R0063/annotation2053/range2053/1
type: Range
persistent identity: range2053
display id: range2053
version: 1
direction: direction/forward
top level: lux pL
start: 89
end: 94
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2052
display id: annotation2052
version: 1
top level: lux pL
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0063/annotation2052/range2052/1

https://synbiohub.org/public/igem/BBa_R0063/annotation2052/range2052/1
type: Range
persistent identity: range2052
display id: range2052
version: 1
direction: direction/forward
top level: lux pL
start: 115
end: 122
orientation: inline

Putative LuxR/HSL
type: SequenceAnnotation
persistent identity: annotation2055
display id: annotation2055
version: 1
top level: lux pL
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0063/annotation2055/range2055/1

https://synbiohub.org/public/igem/BBa_R0063/annotation2055/range2055/1
type: Range
persistent identity: range2055
display id: range2055
version: 1
direction: direction/forward
top level: lux pL
start: 130
end: 149
orientation: inline

BBa_K091101
type: ComponentDefinition
persistent identity: BBa K091101
display id: BBa_K091101
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pTet_Lac hybrid promoter
creator: Erin Feeney
created: 2008-05-28T11:00:00Z
modified: 2015-05-08T01:08:37Z
discontinued: false
dominant: false
experience: experience/None
group u list: _191_
m user id: 0
owner id: 3067
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This promoter is repressed by tetR and lacI; it contains two binding sites for TetR and one LacI binding site. this part is an AND-gate since transcription occurs only in the presence of both ATC and IPTG.
mutable notes: Also designed by Pallavi Penumetcha and Robert Cool.
mutable provenance: This part consists of the R0040 tetR promoter with the addition of the R0010 lacI binding site.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: lacI binding site
sequence annotation: tetR binding site 2
sequence annotation: tetR binding site 1
sequence annotation: -10
sequence annotation: -35
sequence: https://synbiohub.org/public/igem/BBa_K091101_sequence/1

lacI binding site
type: SequenceAnnotation
persistent identity: annotation1963341
display id: annotation1963341
version: 1
top level: BBa_K091101
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K091101/annotation1963341/range1963341/1

https://synbiohub.org/public/igem/BBa_K091101/annotation1963341/range1963341/1
type: Range
persistent identity: range1963341
display id: range1963341
version: 1
direction: direction/forward
top level: BBa_K091101
start: 49
end: 83
orientation: inline

tetR binding site 2
type: SequenceAnnotation
persistent identity: annotation1963338
display id: annotation1963338
version: 1
top level: BBa_K091101
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K091101/annotation1963338/range1963338/1

https://synbiohub.org/public/igem/BBa_K091101/annotation1963338/range1963338/1
type: Range
persistent identity: range1963338
display id: range1963338
version: 1
direction: direction/forward
top level: BBa_K091101
start: 26
end: 44
orientation: inline

tetR binding site 1
type: SequenceAnnotation
persistent identity: annotation1963337
display id: annotation1963337
version: 1
top level: BBa_K091101
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K091101/annotation1963337/range1963337/1

https://synbiohub.org/public/igem/BBa_K091101/annotation1963337/range1963337/1
type: Range
persistent identity: range1963337
display id: range1963337
version: 1
direction: direction/forward
top level: BBa_K091101
start: 1
end: 19
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1963340
display id: annotation1963340
version: 1
top level: BBa_K091101
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K091101/annotation1963340/range1963340/1

https://synbiohub.org/public/igem/BBa_K091101/annotation1963340/range1963340/1
type: Range
persistent identity: range1963340
display id: range1963340
version: 1
direction: direction/forward
top level: BBa_K091101
start: 43
end: 48
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1963339
display id: annotation1963339
version: 1
top level: BBa_K091101
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K091101/annotation1963339/range1963339/1

https://synbiohub.org/public/igem/BBa_K091101/annotation1963339/range1963339/1
type: Range
persistent identity: range1963339
display id: range1963339
version: 1
direction: direction/forward
top level: BBa_K091101
start: 20
end: 25
orientation: inline

BBa_K1897003
type: ComponentDefinition
persistent identity: BBa K1897003
display id: BBa_K1897003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: HasS antisigma factor coding sequence
creator: Choi Yan Ru
created: 2016-10-08T11:00:00Z
modified: 2016-10-18T01:29:52Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2363_
m user id: 29428
owner id: 29428
owning group id: 9
status: status/Planning
bookmark: false
mutable description: HasR is the membrane-bound receptor in the Has system, a heme acquisition system originally from the Serratia marcescens. The HasR protein is involved in binding with a heme-loaded HasA hemophore to trigger downstream HasS and HasI. It has been shown that the N terminal of HasR is especially crucial for signal transduction. With the heme-loaded hasA bound to hasR, it induces a conformational change in HasR that causes the activation of HasS, an anti-sigma factor. HasS then releases HasI (sigma factor) that was initially bound to it and HasI ultimately is able to trigger expression of genes under control of pHas (part number).
mutable notes: NIL
mutable provenance: The HasS gene is originally found in the Serratia marcescens. We obtained the gene sequence from the National Center for Biotechnology Information (NCBI) database (http://www.ncbi.nlm.nih.gov/nuccore/X81195.2) and synthesised the gene.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1897003_sequence/1

BBa_K1486004
type: ComponentDefinition
persistent identity: BBa K1486004
display id: BBa_K1486004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: flexible linker 2x (GGGGS)
creator: iGem EPFL 2014
created: 2014-08-11T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Flexible linker used to fuse two proteins.
mutable notes: Designed considering the flexibility and separation needed between our fused proteins.
mutable provenance: Synthetic.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence: https://synbiohub.org/public/igem/BBa_K1486004_sequence/1

BBa_B1010
type: ComponentDefinition
persistent identity: BBa B1010
display id: BBa_B1010
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Terninator (artificial, large, %T~<10)
creator: Haiyao Huang
created: 2006-09-06T11:00:00Z
modified: 2015-08-31T04:07:21Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _41_
m user id: 0
owner id: 745
owning group id: 41
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Artificial terminator, estimated %T~<10% 8bp stem, 6nt loop Bidirectional, estimated reverse %T~=40%
mutable notes: Bidirectional, with the reverse estimated to be more effective than the forward. Has a polyA tail of 3 residues.
mutable provenance: modified E. coli thr terminator, replaced all A-T pairs in stem with C-G pairs
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: PolyA
sequence annotation: B1010
sequence annotation: modified thr terminator
sequence: https://synbiohub.org/public/igem/BBa_B1010_sequence/1

PolyA
type: SequenceAnnotation
persistent identity: annotation1901016
display id: annotation1901016
version: 1
top level: BBa_B1010
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_B1010/annotation1901016/range1901016/1

https://synbiohub.org/public/igem/BBa_B1010/annotation1901016/range1901016/1
type: Range
persistent identity: range1901016
display id: range1901016
version: 1
direction: direction/forward
top level: BBa_B1010
start: 32
end: 34
orientation: inline

B1010
type: SequenceAnnotation
persistent identity: annotation1901013
display id: annotation1901013
version: 1
top level: BBa_B1010
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_B1010/annotation1901013/range1901013/1

https://synbiohub.org/public/igem/BBa_B1010/annotation1901013/range1901013/1
type: Range
persistent identity: range1901013
display id: range1901013
version: 1
direction: direction/forward
top level: BBa_B1010
start: 1
end: 40
orientation: inline

PolyA
type: SequenceAnnotation
persistent identity: annotation1901015
display id: annotation1901015
version: 1
top level: BBa_B1010
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_B1010/annotation1901015/range1901015/1

https://synbiohub.org/public/igem/BBa_B1010/annotation1901015/range1901015/1
type: Range
persistent identity: range1901015
display id: range1901015
version: 1
direction: direction/reverse
top level: BBa_B1010
start: 7
end: 9
orientation: inline

modified thr terminator
type: SequenceAnnotation
persistent identity: annotation1901014
display id: annotation1901014
version: 1
top level: BBa_B1010
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_B1010/annotation1901014/range1901014/1

https://synbiohub.org/public/igem/BBa_B1010/annotation1901014/range1901014/1
type: Range
persistent identity: range1901014
display id: range1901014
version: 1
direction: direction/forward
top level: BBa_B1010
start: 10
end: 31
orientation: inline

BBa_K1486055
type: ComponentDefinition
persistent identity: BBa K1486055
display id: BBa_K1486055
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CheY/CheZ + split fLuc
creator: EPFL iGem team 2014
created: 2014-10-06T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: to edit
mutable notes: to edit
mutable provenance: to edit
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_B0034
component: BBa_K569017
component: BBa_K1486016
component: BBa_I0500
component: BBa_K1486053
component: BBa_K629003
component: BBa_K1486017
sequence annotation: BBa_K1486053
sequence annotation: BBa_K569017
sequence annotation: BBa_K1486017
sequence annotation: BBa_B0034
sequence annotation: BBa_K1486016
sequence annotation: BBa_I0500
sequence annotation: BBa_K629003
sequence: https://synbiohub.org/public/igem/BBa_K1486055_sequence/1

BBa_B0034
type: Component
persistent identity: component2399881
display id: component2399881
version: 1
top level: BBa_K1486055
access: public
definition: BBa_B0034

BBa_B0034
type: Component
persistent identity: component2399886
display id: component2399886
version: 1
top level: BBa_K1486055
access: public
definition: BBa_B0034

BBa_K569017
type: Component
persistent identity: component2399882
display id: component2399882
version: 1
top level: BBa_K1486055
access: public
definition: https://synbiohub.org/public/igem/BBa_K569017/1

BBa_K1486016
type: Component
persistent identity: component2399884
display id: component2399884
version: 1
top level: BBa_K1486055
access: public
definition: BBa_K1486016

BBa_I0500
type: Component
persistent identity: component2399879
display id: component2399879
version: 1
top level: BBa_K1486055
access: public
definition: pBad/araC

BBa_K1486053
type: Component
persistent identity: component2399883
display id: component2399883
version: 1
top level: BBa_K1486055
access: public
definition: BBa_K1486053

BBa_K1486053
type: Component
persistent identity: component2399894
display id: component2399894
version: 1
top level: BBa_K1486055
access: public
definition: BBa_K1486053

BBa_K629003
type: Component
persistent identity: component2399893
display id: component2399893
version: 1
top level: BBa_K1486055
access: public
definition: https://synbiohub.org/public/igem/BBa_K629003/1

BBa_K1486017
type: Component
persistent identity: component2399895
display id: component2399895
version: 1
top level: BBa_K1486055
access: public
definition: BBa_K1486017

BBa_K1486053
type: SequenceAnnotation
persistent identity: annotation2399894
display id: annotation2399894
version: 1
top level: BBa_K1486055
location: https://synbiohub.org/public/igem/BBa_K1486055/annotation2399894/range2399894/1
component: BBa_K1486053

https://synbiohub.org/public/igem/BBa_K1486055/annotation2399894/range2399894/1
type: Range
persistent identity: range2399894
display id: range2399894
version: 1
direction: direction/reverse
top level: BBa_K1486055
start: 2802
end: 2831
orientation: inline

BBa_K569017
type: SequenceAnnotation
persistent identity: annotation2399882
display id: annotation2399882
version: 1
top level: BBa_K1486055
location: https://synbiohub.org/public/igem/BBa_K1486055/annotation2399882/range2399882/1
component: BBa_K569017

https://synbiohub.org/public/igem/BBa_K1486055/annotation2399882/range2399882/1
type: Range
persistent identity: range2399882
display id: range2399882
version: 1
direction: direction/reverse
top level: BBa_K1486055
start: 1223
end: 1640
orientation: inline

BBa_K1486017
type: SequenceAnnotation
persistent identity: annotation2399895
display id: annotation2399895
version: 1
top level: BBa_K1486055
location: https://synbiohub.org/public/igem/BBa_K1486055/annotation2399895/range2399895/1
component: BBa_K1486017

https://synbiohub.org/public/igem/BBa_K1486055/annotation2399895/range2399895/1
type: Range
persistent identity: range2399895
display id: range2399895
version: 1
direction: direction/reverse
top level: BBa_K1486055
start: 2832
end: 4079
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2399881
display id: annotation2399881
version: 1
top level: BBa_K1486055
location: https://synbiohub.org/public/igem/BBa_K1486055/annotation2399881/range2399881/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1486055/annotation2399881/range2399881/1
type: Range
persistent identity: range2399881
display id: range2399881
version: 1
direction: direction/reverse
top level: BBa_K1486055
start: 1211
end: 1222
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2399886
display id: annotation2399886
version: 1
top level: BBa_K1486055
location: https://synbiohub.org/public/igem/BBa_K1486055/annotation2399886/range2399886/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1486055/annotation2399886/range2399886/1
type: Range
persistent identity: range2399886
display id: range2399886
version: 1
direction: direction/reverse
top level: BBa_K1486055
start: 2145
end: 2156
orientation: inline

BBa_K1486016
type: SequenceAnnotation
persistent identity: annotation2399884
display id: annotation2399884
version: 1
top level: BBa_K1486055
location: https://synbiohub.org/public/igem/BBa_K1486055/annotation2399884/range2399884/1
component: BBa_K1486016

https://synbiohub.org/public/igem/BBa_K1486055/annotation2399884/range2399884/1
type: Range
persistent identity: range2399884
display id: range2399884
version: 1
direction: direction/reverse
top level: BBa_K1486055
start: 1671
end: 2144
orientation: inline

BBa_I0500
type: SequenceAnnotation
persistent identity: annotation2399879
display id: annotation2399879
version: 1
top level: BBa_K1486055
location: https://synbiohub.org/public/igem/BBa_K1486055/annotation2399879/range2399879/1
component: BBa_I0500

https://synbiohub.org/public/igem/BBa_K1486055/annotation2399879/range2399879/1
type: Range
persistent identity: range2399879
display id: range2399879
version: 1
direction: direction/reverse
top level: BBa_K1486055
start: 1
end: 1210
orientation: inline

BBa_K1486053
type: SequenceAnnotation
persistent identity: annotation2399883
display id: annotation2399883
version: 1
top level: BBa_K1486055
location: https://synbiohub.org/public/igem/BBa_K1486055/annotation2399883/range2399883/1
component: BBa_K1486053

https://synbiohub.org/public/igem/BBa_K1486055/annotation2399883/range2399883/1
type: Range
persistent identity: range2399883
display id: range2399883
version: 1
direction: direction/reverse
top level: BBa_K1486055
start: 1641
end: 1670
orientation: inline

BBa_K629003
type: SequenceAnnotation
persistent identity: annotation2399893
display id: annotation2399893
version: 1
top level: BBa_K1486055
location: https://synbiohub.org/public/igem/BBa_K1486055/annotation2399893/range2399893/1
component: BBa_K629003

https://synbiohub.org/public/igem/BBa_K1486055/annotation2399893/range2399893/1
type: Range
persistent identity: range2399893
display id: range2399893
version: 1
direction: direction/reverse
top level: BBa_K1486055
start: 2157
end: 2801
orientation: inline

BBa_K086032
type: ComponentDefinition
persistent identity: BBa K086032
display id: BBa_K086032
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: modified Lutz-Bujard LacO promoter,with alternative sigma factor σ38
creator: Gairik Sachdeva
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:08:37Z
discontinued: false
dominant: false
group u list: _197_
m user id: 0
owner id: 2578
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: modified Lutz-Bujard LacO promoter,with alternative sigma factor σ38
mutable notes: cleaned
mutable provenance: Lac O and Sigma 38 binding sites conserved sequences
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: P38_3
sequence: https://synbiohub.org/public/igem/BBa_K086032_sequence/1

P38_3
type: SequenceAnnotation
persistent identity: annotation1995774
display id: annotation1995774
version: 1
top level: BBa_K086032
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K086032/annotation1995774/range1995774/1

https://synbiohub.org/public/igem/BBa_K086032/annotation1995774/range1995774/1
type: Range
persistent identity: range1995774
display id: range1995774
version: 1
direction: direction/forward
top level: BBa_K086032
start: 1
end: 55
orientation: inline

Ara
type: ComponentDefinition
persistent identity: BBa K808000
display id: BBa_K808000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: araC-Pbad - Arabinose inducible regulatory promoter/repressor unit
creator: Valentina Herbring, Sebastian Palluk, Andreas Schmidt
created: 2012-08-10T11:00:00Z
modified: 2015-05-08T01:13:25Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1065_
m user id: 0
owner id: 11792
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: This promoter can be induced by arabinose. In presens of arabinose the promotor is active.
mutable notes: non
mutable provenance: Guzman LM, Belin D, Carson MJ, Beckwith J. Tight regulation, modulation, and high-level expression by vectors containing the arabinose PBAD promoter. J Bacteriol. 1995 Jul;177(14):4121-30 Khlebnikov A, Skaug T, Keasling JD, modulation of gene expression from the arabinose-inducible araBAD promoter,J Ind Microbiol Biotechnol. 2002 Jul;29(1):34-7.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: AraPromoter
sequence annotation: AraO
sequence annotation: AraC
sequence annotation: AraO1
sequence: https://synbiohub.org/public/igem/BBa_K808000_sequence/1

AraPromoter
type: SequenceAnnotation
persistent identity: annotation2200760
display id: annotation2200760
version: 1
top level: Ara
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K808000/annotation2200760/range2200760/1

https://synbiohub.org/public/igem/BBa_K808000/annotation2200760/range2200760/1
type: Range
persistent identity: range2200760
display id: range2200760
version: 1
direction: direction/forward
top level: Ara
start: 1153
end: 1181
orientation: inline

AraO
type: SequenceAnnotation
persistent identity: annotation2200758
display id: annotation2200758
version: 1
top level: Ara
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K808000/annotation2200758/range2200758/1

https://synbiohub.org/public/igem/BBa_K808000/annotation2200758/range2200758/1
type: Range
persistent identity: range2200758
display id: range2200758
version: 1
direction: direction/forward
top level: Ara
start: 909
end: 924
orientation: inline

AraC
type: SequenceAnnotation
persistent identity: annotation2200757
display id: annotation2200757
version: 1
top level: Ara
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K808000/annotation2200757/range2200757/1

https://synbiohub.org/public/igem/BBa_K808000/annotation2200757/range2200757/1
type: Range
persistent identity: range2200757
display id: range2200757
version: 1
direction: direction/reverse
top level: Ara
start: 1
end: 879
orientation: inline

AraO1
type: SequenceAnnotation
persistent identity: annotation2200759
display id: annotation2200759
version: 1
top level: Ara
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K808000/annotation2200759/range2200759/1

https://synbiohub.org/public/igem/BBa_K808000/annotation2200759/range2200759/1
type: Range
persistent identity: range2200759
display id: range2200759
version: 1
direction: direction/forward
top level: Ara
start: 1066
end: 1077
orientation: inline

BBa_J54200
type: ComponentDefinition
persistent identity: BBa J54200
display id: BBa_J54200
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: lacq_Promoter
creator: Yusaku Nakashima
created: 2006-10-14T11:00:00Z
modified: 2015-08-31T03:48:12Z
discontinued: false
dominant: false
group u list: _76_
m user id: 0
owner id: 762
owning group id: 76
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Promoter that express always.
mutable notes: As promoter
mutable provenance: DNA synthesis
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: lacq Promoter
sequence annotation: BamHI
sequence: https://synbiohub.org/public/igem/BBa_J54200_sequence/1

lacq Promoter
type: SequenceAnnotation
persistent identity: annotation1903215
display id: annotation1903215
version: 1
top level: BBa_J54200
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_J54200/annotation1903215/range1903215/1

https://synbiohub.org/public/igem/BBa_J54200/annotation1903215/range1903215/1
type: Range
persistent identity: range1903215
display id: range1903215
version: 1
direction: direction/forward
top level: BBa_J54200
start: 14
end: 48
orientation: inline

BamHI
type: SequenceAnnotation
persistent identity: annotation1903214
display id: annotation1903214
version: 1
top level: BBa_J54200
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_J54200/annotation1903214/range1903214/1

https://synbiohub.org/public/igem/BBa_J54200/annotation1903214/range1903214/1
type: Range
persistent identity: range1903214
display id: range1903214
version: 1
direction: direction/forward
top level: BBa_J54200
start: 7
end: 13
orientation: inline

BBa_I741019
type: ComponentDefinition
persistent identity: BBa I741019
display id: BBa_I741019
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Right facing promoter (for xylA) controlled by xylR and CRP-cAMP
creator: Lucien Weiss
created: 2007-06-20T11:00:00Z
modified: 2015-08-31T04:08:02Z
discontinued: false
dominant: false
experience: experience/Issues
group u list: _143_
m user id: 0
owner id: 1763
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: naturally the promoter to xylA and xylB
mutable notes: removed RBS cut out several xylR activation sites
mutable provenance: ecoli k12 chromosome
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: CRP-cAMP transcriptional dual regulator activates transcription
sequence annotation: XylR-Xylose transcriptional activator activates transcription
sequence annotation: xylAp
sequence: https://synbiohub.org/public/igem/BBa_I741019_sequence/1

CRP-cAMP transcriptional dual regulator activates transcription
type: SequenceAnnotation
persistent identity: annotation1935193
display id: annotation1935193
version: 1
top level: BBa_I741019
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I741019/annotation1935193/range1935193/1

https://synbiohub.org/public/igem/BBa_I741019/annotation1935193/range1935193/1
type: Range
persistent identity: range1935193
display id: range1935193
version: 1
direction: direction/forward
top level: BBa_I741019
start: 86
end: 107
orientation: inline

XylR-Xylose transcriptional activator activates transcription
type: SequenceAnnotation
persistent identity: annotation1935195
display id: annotation1935195
version: 1
top level: BBa_I741019
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I741019/annotation1935195/range1935195/1

https://synbiohub.org/public/igem/BBa_I741019/annotation1935195/range1935195/1
type: Range
persistent identity: range1935195
display id: range1935195
version: 1
direction: direction/forward
top level: BBa_I741019
start: 69
end: 85
orientation: inline

xylAp
type: SequenceAnnotation
persistent identity: annotation1935191
display id: annotation1935191
version: 1
top level: BBa_I741019
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I741019/annotation1935191/range1935191/1

https://synbiohub.org/public/igem/BBa_I741019/annotation1935191/range1935191/1
type: Range
persistent identity: range1935191
display id: range1935191
version: 1
direction: direction/reverse
top level: BBa_I741019
start: 76
end: 117
orientation: inline

XylR-Xylose transcriptional activator activates transcription
type: SequenceAnnotation
persistent identity: annotation1935211
display id: annotation1935211
version: 1
top level: BBa_I741019
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I741019/annotation1935211/range1935211/1

https://synbiohub.org/public/igem/BBa_I741019/annotation1935211/range1935211/1
type: Range
persistent identity: range1935211
display id: range1935211
version: 1
direction: direction/reverse
top level: BBa_I741019
start: 48
end: 64
orientation: inline

BBa_I760005
type: ComponentDefinition
persistent identity: BBa I760005
display id: BBa_I760005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Cu-sensitive promoter
creator: Gennady Zakharov
created: 2007-08-19T11:00:00Z
modified: 2015-08-31T04:08:08Z
discontinued: false
dominant: false
experience: experience/None
group u list: _148_
m user id: 0
owner id: 2080
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Promoter from the copper-sensetive CusR/CusS two component signal system. (E.Coli)
mutable notes: Sequence from the NCBI database, with added BioBricks prefix and suffix.
mutable provenance: http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I760005_sequence/1

creA RBS
type: ComponentDefinition
persistent identity: BBa J64907
display id: BBa_J64907
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: creA RBS
creator: Ryan Ritterson
created: 2007-03-19T12:00:00Z
modified: 2015-05-08T01:08:17Z
discontinued: false
dominant: false
experience: experience/None
group u list: _98_
m user id: 0
owner id: 1422
owning group id: 98
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: ribosome binding site for creA gene in the E Coli cre operon
mutable notes: natural sequence, no design.
mutable provenance: E Coli strain K12
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J64907_sequence/1

BBa_K1165002
type: ComponentDefinition
persistent identity: BBa K1165002
display id: BBa_K1165002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PenI-cleaving Ribozyme 1 of XOR Gate 1
creator: iGEM Concordia 2013
created: 2013-09-26T11:00:00Z
modified: 2015-05-08T01:09:33Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1477_
m user id: 0
owner id: 18237
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is the DNA sequence of a Y-type hammerhead ribozyme that targets and cleaves the PenI repressor (Part BBa_C0074). This ribozyme has target-binding flanking arms adjacent to the Y-type hammerhead catalytic core and synthetic extended arms located towards the 5' and 3' ends. The catalytic core allows for the sequence-specific cleavage of sites within the coding sequence of PenI. Hammerhead ribozymes are able to cleave at any NUH within an RNA sequence, where H represent any nucleotide except guanine (Mir 2001), although with GUC as the hammerhead consensus cleavage site (Lieber, 1995). The binding of the flanking arms adjacent to the hammerhead catalytic core favours the recognition and cleavage of only one target site. Additional secondary structure within the flanking arms was detected to a small degree by using IDT OligoAnalyzer's Hairpin tool (Owczarzy 2008) as is depicted below. [image] The synthetic extended arms are designed to bind to the complementary extended arms of another similar PenI-cleaving ribozyme (Part _____), but do not contribute to any additional secondary structure as confirmed via the Hairpin tool in IDT's OligoAnalyzer (Owczarzy 2008), as both arms of a particular ribozyme only contain a purine and pyrimidine pair that do not bind to each other (in this case, A and G). The two-nucleotide extended arms are also staggered in number to avoid slippage in binding and bind to its complement pair at significantly higher melting temperatures than the adjacent flanking arms. This feature allows for the sequestering of the two ribozymes when both are present and significantly decreasing any ribozyme activity. Regions of complementarity between the two target-binding regions of both ribozymes are also present to ensure that the functional catalytic cores are not able to cleave their mRNA targets, even when bound. The characteristics of this expressed pair of ribozymes were considered to create a functional RNA-based XOR logic gate. References Mir, A…
mutable notes: Although this particular part is likely to only cleave the PenI repressor mRNA due to its sequence specificity, the simple in silico design of this ribozyme allows for the cleavage of virtually any RNA sequence containing a cleave site (NUH). This particular sequence was obtained by first scanning our target PenI mRNA for the optimal cut site GUC. The 10 nucleotides surrounding this cut site were obtained and the complement of this area was generated for the ribozyme flanking arms. The length of this region was decided by ideal melting temperature (51.4 degrees Celsius). Additional synthetic extended arms were subsequently added to the 5' and 3' ends of the sequence for binding to the complement ribozyme in an XOR gate. This region of non-pairing purines and pyrimidines was staggered in base repeats to avoid slippage of binding and was defined in length by significantly higher melting temperatures (approximately 68 degrees Celsius). Finally, the catalytic core of the Y-type hammerhead ribozyme was introduced at the appropriate region. Throughout this entire process, secondary was continually checked and minimized using IDT OligoAnalyzer's Hairpin tool (Owczarzy 2008). The complete ribozyme sequences was also BLASTed again the E.coli genome, which was our chassis and yielded insignificant results. Other factors such as differential length or target binding regions due to varying melting temperature and GC content were also considered during the in silico design of this ribozyme and its pair in the XOR logic gate. References Owczarzy, R. et al. IDT SciTools: a suite for analysis and design of nucleic acid oligomers. Nucleic Acids Res 36, W163???W169 (2008).
mutable provenance: The target-binding flanking arms of the ribozyme were obtained from the complement of the PenI Repressor sequence.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000834
role: partType/RNA
sequence annotation: TAA Bump
sequence annotation: Target-Binding Arms
sequence annotation: Synthetic Extended Arms
sequence annotation: Catalytic Core
sequence: https://synbiohub.org/public/igem/BBa_K1165002_sequence/1

TAA Bump
type: SequenceAnnotation
persistent identity: annotation2364286
display id: annotation2364286
version: 1
top level: BBa_K1165002
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1165002/annotation2364286/range2364286/1

https://synbiohub.org/public/igem/BBa_K1165002/annotation2364286/range2364286/1
type: Range
persistent identity: range2364286
display id: range2364286
version: 1
direction: direction/forward
top level: BBa_K1165002
start: 36
end: 38
orientation: inline

Target-Binding Arms
type: SequenceAnnotation
persistent identity: annotation2364285
display id: annotation2364285
version: 1
top level: BBa_K1165002
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1165002/annotation2364285/range2364285/1

https://synbiohub.org/public/igem/BBa_K1165002/annotation2364285/range2364285/1
type: Range
persistent identity: range2364285
display id: range2364285
version: 1
direction: direction/forward
top level: BBa_K1165002
start: 39
end: 43
orientation: inline

Synthetic Extended Arms
type: SequenceAnnotation
persistent identity: annotation2364282
display id: annotation2364282
version: 1
top level: BBa_K1165002
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1165002/annotation2364282/range2364282/1

https://synbiohub.org/public/igem/BBa_K1165002/annotation2364282/range2364282/1
type: Range
persistent identity: range2364282
display id: range2364282
version: 1
direction: direction/forward
top level: BBa_K1165002
start: 1
end: 30
orientation: inline

Synthetic Extended Arms
type: SequenceAnnotation
persistent identity: annotation2364283
display id: annotation2364283
version: 1
top level: BBa_K1165002
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1165002/annotation2364283/range2364283/1

https://synbiohub.org/public/igem/BBa_K1165002/annotation2364283/range2364283/1
type: Range
persistent identity: range2364283
display id: range2364283
version: 1
direction: direction/forward
top level: BBa_K1165002
start: 82
end: 111
orientation: inline

Catalytic Core
type: SequenceAnnotation
persistent identity: annotation2364287
display id: annotation2364287
version: 1
top level: BBa_K1165002
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1165002/annotation2364287/range2364287/1

https://synbiohub.org/public/igem/BBa_K1165002/annotation2364287/range2364287/1
type: Range
persistent identity: range2364287
display id: range2364287
version: 1
direction: direction/forward
top level: BBa_K1165002
start: 44
end: 69
orientation: inline

Target-Binding Arms
type: SequenceAnnotation
persistent identity: annotation2364284
display id: annotation2364284
version: 1
top level: BBa_K1165002
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1165002/annotation2364284/range2364284/1

https://synbiohub.org/public/igem/BBa_K1165002/annotation2364284/range2364284/1
type: Range
persistent identity: range2364284
display id: range2364284
version: 1
direction: direction/forward
top level: BBa_K1165002
start: 31
end: 35
orientation: inline

Target-Binding Arms
type: SequenceAnnotation
persistent identity: annotation2364288
display id: annotation2364288
version: 1
top level: BBa_K1165002
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1165002/annotation2364288/range2364288/1

https://synbiohub.org/public/igem/BBa_K1165002/annotation2364288/range2364288/1
type: Range
persistent identity: range2364288
display id: range2364288
version: 1
direction: direction/forward
top level: BBa_K1165002
start: 70
end: 81
orientation: inline

BBa_K1486035
type: ComponentDefinition
persistent identity: BBa K1486035
display id: BBa_K1486035
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Yeast Optimized sfGFP-C + ADH1 terminator + CaUra3 Cassette
creator: EPFL iGem team 2014
created: 2014-09-26T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: To edit
mutable notes: To edit
mutable provenance: To edit
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000324
role: partType/Tag
component: BBa_K1486025
component: BBa_K1486031
component: BBa_K1486034
component: BBa_K1486037
sequence annotation: BBa_K1486034
sequence annotation: BBa_K1486037
sequence annotation: BBa_K1486031
sequence annotation: BBa_K1486025
sequence: https://synbiohub.org/public/igem/BBa_K1486035_sequence/1

BBa_K1486025
type: Component
persistent identity: component2418331
display id: component2418331
version: 1
top level: BBa_K1486035
access: public
definition: BBa_K1486025

BBa_K1486031
type: Component
persistent identity: component2418335
display id: component2418335
version: 1
top level: BBa_K1486035
access: public
definition: BBa_K1486031

BBa_K1486034
type: Component
persistent identity: component2418330
display id: component2418330
version: 1
top level: BBa_K1486035
access: public
definition: BBa_K1486034

BBa_K1486037
type: Component
persistent identity: component2418328
display id: component2418328
version: 1
top level: BBa_K1486035
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486037/1

BBa_K1486034
type: SequenceAnnotation
persistent identity: annotation2418330
display id: annotation2418330
version: 1
top level: BBa_K1486035
location: https://synbiohub.org/public/igem/BBa_K1486035/annotation2418330/range2418330/1
component: BBa_K1486034

https://synbiohub.org/public/igem/BBa_K1486035/annotation2418330/range2418330/1
type: Range
persistent identity: range2418330
display id: range2418330
version: 1
direction: direction/reverse
top level: BBa_K1486035
start: 40
end: 114
orientation: inline

BBa_K1486037
type: SequenceAnnotation
persistent identity: annotation2418328
display id: annotation2418328
version: 1
top level: BBa_K1486035
location: https://synbiohub.org/public/igem/BBa_K1486035/annotation2418328/range2418328/1
component: BBa_K1486037

https://synbiohub.org/public/igem/BBa_K1486035/annotation2418328/range2418328/1
type: Range
persistent identity: range2418328
display id: range2418328
version: 1
direction: direction/reverse
top level: BBa_K1486035
start: 1
end: 39
orientation: inline

BBa_K1486031
type: SequenceAnnotation
persistent identity: annotation2418335
display id: annotation2418335
version: 1
top level: BBa_K1486035
location: https://synbiohub.org/public/igem/BBa_K1486035/annotation2418335/range2418335/1
component: BBa_K1486031

https://synbiohub.org/public/igem/BBa_K1486035/annotation2418335/range2418335/1
type: Range
persistent identity: range2418335
display id: range2418335
version: 1
direction: direction/reverse
top level: BBa_K1486035
start: 303
end: 1662
orientation: inline

BBa_K1486025
type: SequenceAnnotation
persistent identity: annotation2418331
display id: annotation2418331
version: 1
top level: BBa_K1486035
location: https://synbiohub.org/public/igem/BBa_K1486035/annotation2418331/range2418331/1
component: BBa_K1486025

https://synbiohub.org/public/igem/BBa_K1486035/annotation2418331/range2418331/1
type: Range
persistent identity: range2418331
display id: range2418331
version: 1
direction: direction/reverse
top level: BBa_K1486035
start: 115
end: 302
orientation: inline

BBa_I751501
type: ComponentDefinition
persistent identity: BBa I751501
display id: BBa_I751501
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: plux-cI hybrid promoter
creator: Kenichiro Iwasaki
created: 2007-10-24T11:00:00Z
modified: 2015-08-31T04:08:06Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _151_
m user id: 0
owner id: 2188
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: sense TWO INPUTS, activation by AHL and repression by LacI.
mutable notes: Based on the AHL regulated BioBrick part R0062 and the previous work on LacI regulated promoter (ref. 1), we designed a new promoter regulated by AHL and LacI. Since this promoter is a kind of a "HYBRID" of the two, we call it HYBRID PROMOTER. Compared with existing BioBrick hybrid promoter R0065, this promoter is different in that its sequence is much shorter with lux box and -35 region overlapping, and that it is repressed by LacI, not cI. Also, the output is clearly detected as shown in Fig. 3.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: luxR binding
sequence annotation: OR2
sequence annotation: OR1
sequence annotation: -35
sequence annotation: -10
sequence annotation: plux-cI hybrid promoter
sequence: https://synbiohub.org/public/igem/BBa_I751501_sequence/1

luxR binding
type: SequenceAnnotation
persistent identity: annotation1955482
display id: annotation1955482
version: 1
top level: BBa_I751501
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I751501/annotation1955482/range1955482/1

https://synbiohub.org/public/igem/BBa_I751501/annotation1955482/range1955482/1
type: Range
persistent identity: range1955482
display id: range1955482
version: 1
direction: direction/forward
top level: BBa_I751501
start: 1
end: 18
orientation: inline

OR2
type: SequenceAnnotation
persistent identity: annotation1955481
display id: annotation1955481
version: 1
top level: BBa_I751501
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I751501/annotation1955481/range1955481/1

https://synbiohub.org/public/igem/BBa_I751501/annotation1955481/range1955481/1
type: Range
persistent identity: range1955481
display id: range1955481
version: 1
direction: direction/forward
top level: BBa_I751501
start: 26
end: 41
orientation: inline

OR1
type: SequenceAnnotation
persistent identity: annotation1955477
display id: annotation1955477
version: 1
top level: BBa_I751501
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I751501/annotation1955477/range1955477/1

https://synbiohub.org/public/igem/BBa_I751501/annotation1955477/range1955477/1
type: Range
persistent identity: range1955477
display id: range1955477
version: 1
direction: direction/forward
top level: BBa_I751501
start: 48
end: 66
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1955479
display id: annotation1955479
version: 1
top level: BBa_I751501
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I751501/annotation1955479/range1955479/1

https://synbiohub.org/public/igem/BBa_I751501/annotation1955479/range1955479/1
type: Range
persistent identity: range1955479
display id: range1955479
version: 1
direction: direction/forward
top level: BBa_I751501
start: 19
end: 25
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1955480
display id: annotation1955480
version: 1
top level: BBa_I751501
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I751501/annotation1955480/range1955480/1

https://synbiohub.org/public/igem/BBa_I751501/annotation1955480/range1955480/1
type: Range
persistent identity: range1955480
display id: range1955480
version: 1
direction: direction/forward
top level: BBa_I751501
start: 42
end: 47
orientation: inline

plux-cI hybrid promoter
type: SequenceAnnotation
persistent identity: annotation1955478
display id: annotation1955478
version: 1
top level: BBa_I751501
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I751501/annotation1955478/range1955478/1

https://synbiohub.org/public/igem/BBa_I751501/annotation1955478/range1955478/1
type: Range
persistent identity: range1955478
display id: range1955478
version: 1
direction: direction/forward
top level: BBa_I751501
start: 19
end: 66
orientation: inline

BBa_J56012
type: ComponentDefinition
persistent identity: BBa J56012
display id: BBa_J56012
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Invertible sequence of dna includes Ptrc promoter
creator: Matt Eames
created: 2006-10-31T12:00:00Z
modified: 2015-08-31T03:22:59Z
discontinued: false
dominant: false
group u list: _79_98_
m user id: 0
owner id: 1132
owning group id: 98
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Seuqence is inverted by the FimE protein. Inside recognition sequence is a Ptrc promoter sequence. Switch is irreversible.
mutable notes: None
mutable provenance: Synthesized by DNA 2.0
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J56012_sequence/1

NAND2-7
type: ComponentDefinition
persistent identity: BBa I732304
display id: BBa_I732304
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: NAND Candidate (U073O22D001O47)
creator: Ma Rui
created: 2007-10-02T11:00:00Z
modified: 2015-08-31T04:07:58Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1592
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: U97NUL D001O44
mutable notes: .
mutable provenance: .
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732304_sequence/1

BBa_J64018
type: ComponentDefinition
persistent identity: BBa J64018
display id: BBa_J64018
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: invJ RBS
creator: Dan Widmaier
created: 2008-01-16T12:00:00Z
modified: 2015-08-31T01:56:26Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _98_
m user id: 0
owner id: 2407
owning group id: 98
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This part is the natural RBS associated with the invJ protein in Salmonella Typhimurium LT2.
mutable notes: This part was not designed with compliance to biobrick standard assembly in mind.
mutable provenance: Salmonella Typhimurium LT2 Genomic DNA
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: invJ RBS
sequence: https://synbiohub.org/public/igem/BBa_J64018_sequence/1

invJ RBS
type: SequenceAnnotation
persistent identity: annotation1959617
display id: annotation1959617
version: 1
top level: BBa_J64018
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_J64018/annotation1959617/range1959617/1

https://synbiohub.org/public/igem/BBa_J64018/annotation1959617/range1959617/1
type: Range
persistent identity: range1959617
display id: range1959617
version: 1
direction: direction/forward
top level: BBa_J64018
start: 1
end: 17
orientation: inline

BBa_K110008
type: ComponentDefinition
persistent identity: BBa K110008
display id: BBa_K110008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: A-Cell Promoter MFA1
creator: James DiCarlo
created: 2008-05-29T11:00:00Z
modified: 2015-05-08T01:09:09Z
discontinued: false
dominant: false
experience: experience/None
group u list: _201_
m user id: 0
owner id: 2669
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This promoter was isolated by taking 500bp upstream of the MFA1 gene of S. cerevisiae. This promoter will be activated when A factor is expressed in an A haploid yeast cell.
mutable notes: This gene is on the Watson strand of the entered sequence.
mutable provenance: SGD
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: STE12
sequence annotation: MATalpha2
sequence annotation: MFA1 Promoter
sequence annotation: MCM1
sequence: https://synbiohub.org/public/igem/BBa_K110008_sequence/1

STE12
type: SequenceAnnotation
persistent identity: annotation1981090
display id: annotation1981090
version: 1
top level: BBa_K110008
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K110008/annotation1981090/range1981090/1

https://synbiohub.org/public/igem/BBa_K110008/annotation1981090/range1981090/1
type: Range
persistent identity: range1981090
display id: range1981090
version: 1
direction: direction/forward
top level: BBa_K110008
start: 302
end: 309
orientation: inline

MATalpha2
type: SequenceAnnotation
persistent identity: annotation1981087
display id: annotation1981087
version: 1
top level: BBa_K110008
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K110008/annotation1981087/range1981087/1

https://synbiohub.org/public/igem/BBa_K110008/annotation1981087/range1981087/1
type: Range
persistent identity: range1981087
display id: range1981087
version: 1
direction: direction/forward
top level: BBa_K110008
start: 267
end: 276
orientation: inline

MFA1 Promoter
type: SequenceAnnotation
persistent identity: annotation1981086
display id: annotation1981086
version: 1
top level: BBa_K110008
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K110008/annotation1981086/range1981086/1

https://synbiohub.org/public/igem/BBa_K110008/annotation1981086/range1981086/1
type: Range
persistent identity: range1981086
display id: range1981086
version: 1
direction: direction/forward
top level: BBa_K110008
start: 1
end: 501
orientation: inline

MCM1
type: SequenceAnnotation
persistent identity: annotation1981088
display id: annotation1981088
version: 1
top level: BBa_K110008
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K110008/annotation1981088/range1981088/1

https://synbiohub.org/public/igem/BBa_K110008/annotation1981088/range1981088/1
type: Range
persistent identity: range1981088
display id: range1981088
version: 1
direction: direction/forward
top level: BBa_K110008
start: 277
end: 287
orientation: inline

MATalpha2
type: SequenceAnnotation
persistent identity: annotation1981089
display id: annotation1981089
version: 1
top level: BBa_K110008
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K110008/annotation1981089/range1981089/1

https://synbiohub.org/public/igem/BBa_K110008/annotation1981089/range1981089/1
type: Range
persistent identity: range1981089
display id: range1981089
version: 1
direction: direction/forward
top level: BBa_K110008
start: 289
end: 297
orientation: inline

BBa_R0183
type: ComponentDefinition
persistent identity: BBa R0183
display id: BBa_R0183
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: T7 RNAP promoter
creator: Barry Canton
created: 2005-09-25T11:00:00Z
modified: 2015-05-08T01:14:15Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_6_
m user id: 0
owner id: 135
owning group id: 6
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: T7 promoter with an A->C mutation at the -16 position of the consensus sequence
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_R0183_sequence/1

p(osmY)
type: ComponentDefinition
persistent identity: BBa J45993
display id: BBa_J45993
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Minimal stationary phase osmY promoter
creator: Stephen Payne
created: 2006-07-10T11:00:00Z
modified: 2015-08-31T04:08:50Z
discontinued: false
dominant: false
experience: experience/Fails
group u list: _84_
m user id: 0
owner id: 642
owning group id: 84
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: osmY's promoter is active in stationary phase and under high osmotic pressure conditions.
mutable notes: Truncated Forward Primer: 5'- GTT TCT TCG AAT TCG CGG CCG CTT CTA GGCT TAT GTT TTC GCT GAT ATC - 3' Total Length: 49 bp Annealing Length: 21 bp GC Content: 38.1% Melting Temperature: 49.5 degrees C hairpin deltaG: -2.66 kcal/mol self dimer deltaG: -99.97 kcal/mol Reverse Primer: 5'-GTT TCT TCC TGC AGC GGC CGC TAC TAG TAT TGT TAA ATA TAG ATC ACA ATT TTG- 3' Total Length: 54 bp Annealing Length: 25 bp GC Content: 20.0% Melting Temperature: 46.4 degrees C hairpin deltaG: -2.86 kcal/mol self dimer deltaG: -99.44 kcal/mol heterodimer deltaG with Conserved Forward Primer: -99.97 kcal/mol
mutable provenance: This part was PCRed out of E. coli's genome.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -10
sequence annotation: -35
sequence: https://synbiohub.org/public/igem/BBa_J45993_sequence/1

-10
type: SequenceAnnotation
persistent identity: annotation1883647
display id: annotation1883647
version: 1
top level: p(osmY)
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_J45993/annotation1883647/range1883647/1

https://synbiohub.org/public/igem/BBa_J45993/annotation1883647/range1883647/1
type: Range
persistent identity: range1883647
display id: range1883647
version: 1
direction: direction/forward
top level: p(osmY)
start: 46
end: 51
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1883648
display id: annotation1883648
version: 1
top level: p(osmY)
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_J45993/annotation1883648/range1883648/1

https://synbiohub.org/public/igem/BBa_J45993/annotation1883648/range1883648/1
type: Range
persistent identity: range1883648
display id: range1883648
version: 1
direction: direction/forward
top level: p(osmY)
start: 22
end: 27
orientation: inline

pUV
type: ComponentDefinition
persistent identity: BBa I765001
display id: BBa_I765001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: UV promoter
creator: iGEM Colombian Team 2007
created: 2007-10-25T11:00:00Z
modified: 2015-08-31T04:08:09Z
discontinued: false
dominant: false
experience: experience/None
group u list: _118_
m user id: 0
owner id: 2266
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This device is regulated by UV irradiation
mutable notes: None
mutable provenance: Genomic sequence
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: promoter
sequence: https://synbiohub.org/public/igem/BBa_I765001_sequence/1

promoter
type: SequenceAnnotation
persistent identity: annotation1955941
display id: annotation1955941
version: 1
top level: pUV
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I765001/annotation1955941/range1955941/1

https://synbiohub.org/public/igem/BBa_I765001/annotation1955941/range1955941/1
type: Range
persistent identity: range1955941
display id: range1955941
version: 1
direction: direction/forward
top level: pUV
start: 1
end: 76
orientation: inline

BBa_K1486025
type: ComponentDefinition
persistent identity: BBa K1486025
display id: BBa_K1486025
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: ADH1 Terminator
creator: EPFL iGem team 2014
created: 2014-09-25T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: To edit
mutable notes: To edit
mutable provenance: To edit
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: Adh1 terminator
sequence: https://synbiohub.org/public/igem/BBa_K1486025_sequence/1

Adh1 terminator
type: SequenceAnnotation
persistent identity: annotation2418776
display id: annotation2418776
version: 1
top level: BBa_K1486025
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1486025/annotation2418776/range2418776/1

https://synbiohub.org/public/igem/BBa_K1486025/annotation2418776/range2418776/1
type: Range
persistent identity: range2418776
display id: range2418776
version: 1
direction: direction/forward
top level: BBa_K1486025
start: 1
end: 188
orientation: inline

T7 0.3
type: ComponentDefinition
persistent identity: BBa B2001
display id: BBa_B2001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: T7 RBS 0.3
creator: Heather Keller
created: 2006-09-26T11:00:00Z
modified: 2015-08-31T04:07:21Z
discontinued: false
dominant: true
experience: experience/None
group u list: _11_
m user id: 0
owner id: 571
owning group id: 10
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Test RBS from T7 that may be used in E.coli
mutable notes: None
mutable provenance: T7+ genome RBS upstream of gene 0.3
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: SD region
sequence: https://synbiohub.org/public/igem/BBa_B2001_sequence/1

SD region
type: SequenceAnnotation
persistent identity: annotation1902503
display id: annotation1902503
version: 1
top level: T7 0.3
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_B2001/annotation1902503/range1902503/1

https://synbiohub.org/public/igem/BBa_B2001/annotation1902503/range1902503/1
type: Range
persistent identity: range1902503
display id: range1902503
version: 1
direction: direction/forward
top level: T7 0.3
start: 8
end: 12
orientation: inline

BBa_K1072003
type: ComponentDefinition
persistent identity: BBa K1072003
display id: BBa_K1072003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Lux pL controlled LuxR+Lux pR autoinduceing luxI(lva tag)+Lux pR controlled Ndh
creator: Yichong wang, Zhiwei li, Xihao liao and Junjie zhang
created: 2013-09-05T11:00:00Z
modified: 2015-05-08T01:09:01Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1381_
m user id: 0
owner id: 16002
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: BBa_K1072003 is a composite device, including LuxI，LuxR and Ndh genes. LuxR， controlled by Lux pI, generate LuxR protein, which will combined with AHL and activate Lux pR promotor. that complex increases the transcriptional rate of the lux pR promotor while also decrease the transceiptional rate of the Lux pL promoter. LuxI is the generator of AHL controlled by Lux pR promoter. Ndh was activated by AHL and LuxR complex and yields H2O2;
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_R0062
component: BBa_B0012
component: BBa_C0062
component: BBa_C0061
component: BBa_B0034
component: BBa_B0010
component: BBa_K1072001
component: BBa_R0063
sequence annotation: BBa_C0061
sequence annotation: BBa_B0012
sequence annotation: BBa_R0063
sequence annotation: BBa_R0062
sequence annotation: BBa_K1072001
sequence annotation: BBa_B0034
sequence annotation: BBa_B0010
sequence annotation: BBa_C0062
sequence: https://synbiohub.org/public/igem/BBa_K1072003_sequence/1

BBa_R0062
type: Component
persistent identity: component2335177
display id: component2335177
version: 1
top level: BBa_K1072003
access: public
definition: lux pR

BBa_B0012
type: Component
persistent identity: component2335189
display id: component2335189
version: 1
top level: BBa_K1072003
access: public
definition: BBa_B0012

BBa_C0062
type: Component
persistent identity: component2335166
display id: component2335166
version: 1
top level: BBa_K1072003
access: public
definition: https://synbiohub.org/public/igem/BBa_C0062/1

BBa_C0061
type: Component
persistent identity: component2335184
display id: component2335184
version: 1
top level: BBa_K1072003
access: public
definition: https://synbiohub.org/public/igem/BBa_C0061/1

BBa_B0034
type: Component
persistent identity: component2335182
display id: component2335182
version: 1
top level: BBa_K1072003
access: public
definition: BBa_B0034

BBa_B0010
type: Component
persistent identity: component2335170
display id: component2335170
version: 1
top level: BBa_K1072003
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0034
type: Component
persistent identity: component2335199
display id: component2335199
version: 1
top level: BBa_K1072003
access: public
definition: BBa_B0034

BBa_K1072001
type: Component
persistent identity: component2335201
display id: component2335201
version: 1
top level: BBa_K1072003
access: public
definition: https://synbiohub.org/public/igem/BBa_K1072001/1

BBa_R0062
type: Component
persistent identity: component2335194
display id: component2335194
version: 1
top level: BBa_K1072003
access: public
definition: lux pR

BBa_R0063
type: Component
persistent identity: component2335157
display id: component2335157
version: 1
top level: BBa_K1072003
access: public
definition: lux pL

BBa_B0010
type: Component
persistent identity: component2335202
display id: component2335202
version: 1
top level: BBa_K1072003
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0012
type: Component
persistent identity: component2335172
display id: component2335172
version: 1
top level: BBa_K1072003
access: public
definition: BBa_B0012

BBa_B0012
type: Component
persistent identity: component2335204
display id: component2335204
version: 1
top level: BBa_K1072003
access: public
definition: BBa_B0012

BBa_B0010
type: Component
persistent identity: component2335187
display id: component2335187
version: 1
top level: BBa_K1072003
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0034
type: Component
persistent identity: component2335163
display id: component2335163
version: 1
top level: BBa_K1072003
access: public
definition: BBa_B0034

BBa_C0061
type: SequenceAnnotation
persistent identity: annotation2335184
display id: annotation2335184
version: 1
top level: BBa_K1072003
location: https://synbiohub.org/public/igem/BBa_K1072003/annotation2335184/range2335184/1
component: BBa_C0061

https://synbiohub.org/public/igem/BBa_K1072003/annotation2335184/range2335184/1
type: Range
persistent identity: range2335184
display id: range2335184
version: 1
direction: direction/forward
top level: BBa_K1072003
start: 1185
end: 1802
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2335189
display id: annotation2335189
version: 1
top level: BBa_K1072003
location: https://synbiohub.org/public/igem/BBa_K1072003/annotation2335189/range2335189/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K1072003/annotation2335189/range2335189/1
type: Range
persistent identity: range2335189
display id: range2335189
version: 1
direction: direction/forward
top level: BBa_K1072003
start: 1924
end: 1964
orientation: inline

BBa_R0063
type: SequenceAnnotation
persistent identity: annotation2335157
display id: annotation2335157
version: 1
top level: BBa_K1072003
location: https://synbiohub.org/public/igem/BBa_K1072003/annotation2335157/range2335157/1
component: BBa_R0063

https://synbiohub.org/public/igem/BBa_K1072003/annotation2335157/range2335157/1
type: Range
persistent identity: range2335157
display id: range2335157
version: 1
direction: direction/forward
top level: BBa_K1072003
start: 1
end: 151
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2335204
display id: annotation2335204
version: 1
top level: BBa_K1072003
location: https://synbiohub.org/public/igem/BBa_K1072003/annotation2335204/range2335204/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K1072003/annotation2335204/range2335204/1
type: Range
persistent identity: range2335204
display id: range2335204
version: 1
direction: direction/forward
top level: BBa_K1072003
start: 3457
end: 3497
orientation: inline

BBa_R0062
type: SequenceAnnotation
persistent identity: annotation2335194
display id: annotation2335194
version: 1
top level: BBa_K1072003
location: https://synbiohub.org/public/igem/BBa_K1072003/annotation2335194/range2335194/1
component: BBa_R0062

https://synbiohub.org/public/igem/BBa_K1072003/annotation2335194/range2335194/1
type: Range
persistent identity: range2335194
display id: range2335194
version: 1
direction: direction/forward
top level: BBa_K1072003
start: 1973
end: 2027
orientation: inline

BBa_K1072001
type: SequenceAnnotation
persistent identity: annotation2335201
display id: annotation2335201
version: 1
top level: BBa_K1072003
location: https://synbiohub.org/public/igem/BBa_K1072003/annotation2335201/range2335201/1
component: BBa_K1072001

https://synbiohub.org/public/igem/BBa_K1072003/annotation2335201/range2335201/1
type: Range
persistent identity: range2335201
display id: range2335201
version: 1
direction: direction/reverse
top level: BBa_K1072003
start: 2056
end: 3360
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2335172
display id: annotation2335172
version: 1
top level: BBa_K1072003
location: https://synbiohub.org/public/igem/BBa_K1072003/annotation2335172/range2335172/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K1072003/annotation2335172/range2335172/1
type: Range
persistent identity: range2335172
display id: range2335172
version: 1
direction: direction/forward
top level: BBa_K1072003
start: 1055
end: 1095
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2335182
display id: annotation2335182
version: 1
top level: BBa_K1072003
location: https://synbiohub.org/public/igem/BBa_K1072003/annotation2335182/range2335182/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1072003/annotation2335182/range2335182/1
type: Range
persistent identity: range2335182
display id: range2335182
version: 1
direction: direction/reverse
top level: BBa_K1072003
start: 1167
end: 1178
orientation: inline

BBa_R0062
type: SequenceAnnotation
persistent identity: annotation2335177
display id: annotation2335177
version: 1
top level: BBa_K1072003
location: https://synbiohub.org/public/igem/BBa_K1072003/annotation2335177/range2335177/1
component: BBa_R0062

https://synbiohub.org/public/igem/BBa_K1072003/annotation2335177/range2335177/1
type: Range
persistent identity: range2335177
display id: range2335177
version: 1
direction: direction/forward
top level: BBa_K1072003
start: 1104
end: 1158
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2335170
display id: annotation2335170
version: 1
top level: BBa_K1072003
location: https://synbiohub.org/public/igem/BBa_K1072003/annotation2335170/range2335170/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K1072003/annotation2335170/range2335170/1
type: Range
persistent identity: range2335170
display id: range2335170
version: 1
direction: direction/forward
top level: BBa_K1072003
start: 967
end: 1046
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2335202
display id: annotation2335202
version: 1
top level: BBa_K1072003
location: https://synbiohub.org/public/igem/BBa_K1072003/annotation2335202/range2335202/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K1072003/annotation2335202/range2335202/1
type: Range
persistent identity: range2335202
display id: range2335202
version: 1
direction: direction/forward
top level: BBa_K1072003
start: 3369
end: 3448
orientation: inline

BBa_C0062
type: SequenceAnnotation
persistent identity: annotation2335166
display id: annotation2335166
version: 1
top level: BBa_K1072003
location: https://synbiohub.org/public/igem/BBa_K1072003/annotation2335166/range2335166/1
component: BBa_C0062

https://synbiohub.org/public/igem/BBa_K1072003/annotation2335166/range2335166/1
type: Range
persistent identity: range2335166
display id: range2335166
version: 1
direction: direction/forward
top level: BBa_K1072003
start: 178
end: 933
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2335187
display id: annotation2335187
version: 1
top level: BBa_K1072003
location: https://synbiohub.org/public/igem/BBa_K1072003/annotation2335187/range2335187/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K1072003/annotation2335187/range2335187/1
type: Range
persistent identity: range2335187
display id: range2335187
version: 1
direction: direction/forward
top level: BBa_K1072003
start: 1836
end: 1915
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2335199
display id: annotation2335199
version: 1
top level: BBa_K1072003
location: https://synbiohub.org/public/igem/BBa_K1072003/annotation2335199/range2335199/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1072003/annotation2335199/range2335199/1
type: Range
persistent identity: range2335199
display id: range2335199
version: 1
direction: direction/reverse
top level: BBa_K1072003
start: 2036
end: 2047
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2335163
display id: annotation2335163
version: 1
top level: BBa_K1072003
location: https://synbiohub.org/public/igem/BBa_K1072003/annotation2335163/range2335163/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1072003/annotation2335163/range2335163/1
type: Range
persistent identity: range2335163
display id: range2335163
version: 1
direction: direction/reverse
top level: BBa_K1072003
start: 160
end: 171
orientation: inline

BBa_K165035
type: ComponentDefinition
persistent identity: BBa K165035
display id: BBa_K165035
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Gli1 bs + Zif268-HIV bs + mCYC promoter
creator: John Szymanski
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:10:56Z
discontinued: false
dominant: false
group u list: _267_
m user id: 0
owner id: 2512
owning group id: 58
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: The Gli1 and Zif268-HIV transcription factor binding sites on the mCYC minimal promoter (in that order.) Transcription regulation comes from Gli1 and Zif268-HIV binding transcription factors.
mutable notes: -
mutable provenance: Constructed in our lab with parts from Caroline Ajo-Franklin and David Drubin.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
component: BBa_K165030
component: BBa_K165012
sequence annotation: BBa_K165030
sequence annotation: BBa_K165012
sequence: https://synbiohub.org/public/igem/BBa_K165035_sequence/1

BBa_K165030
type: Component
persistent identity: component1995497
display id: component1995497
version: 1
top level: BBa_K165035
access: public
definition: BBa_K165030

BBa_K165012
type: Component
persistent identity: component1995495
display id: component1995495
version: 1
top level: BBa_K165035
access: public
definition: https://synbiohub.org/public/igem/BBa_K165012/1

BBa_K165030
type: SequenceAnnotation
persistent identity: annotation1995497
display id: annotation1995497
version: 1
top level: BBa_K165035
location: https://synbiohub.org/public/igem/BBa_K165035/annotation1995497/range1995497/1
component: BBa_K165030

https://synbiohub.org/public/igem/BBa_K165035/annotation1995497/range1995497/1
type: Range
persistent identity: range1995497
display id: range1995497
version: 1
direction: direction/reverse
top level: BBa_K165035
start: 136
end: 442
orientation: inline

BBa_K165012
type: SequenceAnnotation
persistent identity: annotation1995495
display id: annotation1995495
version: 1
top level: BBa_K165035
location: https://synbiohub.org/public/igem/BBa_K165035/annotation1995495/range1995495/1
component: BBa_K165012

https://synbiohub.org/public/igem/BBa_K165035/annotation1995495/range1995495/1
type: Range
persistent identity: range1995495
display id: range1995495
version: 1
direction: direction/reverse
top level: BBa_K165035
start: 1
end: 127
orientation: inline

BBa_K137124
type: ComponentDefinition
persistent identity: BBa K137124
display id: BBa_K137124
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LacI-repressed promoter A81
creator: Josh Michener
created: 2008-10-07T11:00:00Z
modified: 2015-05-08T01:10:11Z
discontinued: true
dominant: false
experience: experience/None
group u list: _187_
m user id: 0
owner id: 253
owning group id: 6
sample status: Discontinued
status: status/Deleted
bookmark: false
mutable description: LacI-repressible promoter A81 developed by Sidney Cox (http://www.nature.com/msb/journal/v3/n1/full/msb4100187.html). Showed a regulatory range (ON/OFF) of 1.5x10^4.
mutable provenance: Annealed from oligos.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K137124_sequence/1

BBa_K1486009
type: ComponentDefinition
persistent identity: BBa K1486009
display id: BBa_K1486009
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CxpR & Split IFP1.4 [Nterm + Nterm]
creator: iGem EPFL 2014
created: 2014-08-26T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part has two fused proteins downstream of an arabinose-inducible promoter: CpxR-IFP[1] and CpxR-IFP[2]. This construct was made in all orientations: - CpxR-IFP[1] + CpxR-IFP[2] (BBa_K1286008) - IFP[1]-CpxR + CpxR-IFP[2] (BBa_K1486010) - CpxR-IFP[1] + IFP[2]-CpxR (BBa_K1486011)
mutable notes: All orientations were made, so all dimerizations could be tested (see main page for biobrick numbers). Flexible linkers were used for each of the fused proteins, and an RBS was put before each one of them.
mutable provenance: From Michnick Article. To Edit
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K1486006
component: BBa_K1486004
component: BBa_B0034
component: BBa_K1486000
component: BBa_K1486007
component: BBa_K1486052
component: BBa_I0500
sequence annotation: BBa_B0034
sequence annotation: BBa_K1486004
sequence annotation: BBa_K1486000
sequence annotation: BBa_K1486006
sequence annotation: BBa_K1486007
sequence annotation: BBa_K1486052
sequence annotation: BBa_I0500
sequence: https://synbiohub.org/public/igem/BBa_K1486009_sequence/1

BBa_K1486006
type: Component
persistent identity: component2420771
display id: component2420771
version: 1
top level: BBa_K1486009
access: public
definition: BBa_K1486006

BBa_K1486004
type: Component
persistent identity: component2420772
display id: component2420772
version: 1
top level: BBa_K1486009
access: public
definition: BBa_K1486004

BBa_B0034
type: Component
persistent identity: component2420779
display id: component2420779
version: 1
top level: BBa_K1486009
access: public
definition: BBa_B0034

BBa_K1486000
type: Component
persistent identity: component2420788
display id: component2420788
version: 1
top level: BBa_K1486009
access: public
definition: BBa_K1486000

BBa_K1486000
type: Component
persistent identity: component2420776
display id: component2420776
version: 1
top level: BBa_K1486009
access: public
definition: BBa_K1486000

BBa_K1486007
type: Component
persistent identity: component2420756
display id: component2420756
version: 1
top level: BBa_K1486009
access: public
definition: BBa_K1486007

BBa_K1486052
type: Component
persistent identity: component2420777
display id: component2420777
version: 1
top level: BBa_K1486009
access: public
definition: BBa_K1486052

BBa_K1486004
type: Component
persistent identity: component2420758
display id: component2420758
version: 1
top level: BBa_K1486009
access: public
definition: BBa_K1486004

BBa_K1486000
type: Component
persistent identity: component2420766
display id: component2420766
version: 1
top level: BBa_K1486009
access: public
definition: BBa_K1486000

BBa_I0500
type: Component
persistent identity: component2420763
display id: component2420763
version: 1
top level: BBa_K1486009
access: public
definition: pBad/araC

BBa_B0034
type: Component
persistent identity: component2420767
display id: component2420767
version: 1
top level: BBa_K1486009
access: public
definition: BBa_B0034

BBa_K1486004
type: Component
persistent identity: component2420784
display id: component2420784
version: 1
top level: BBa_K1486009
access: public
definition: BBa_K1486004

BBa_K1486007
type: Component
persistent identity: component2420783
display id: component2420783
version: 1
top level: BBa_K1486009
access: public
definition: BBa_K1486007

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2420779
display id: annotation2420779
version: 1
top level: BBa_K1486009
location: https://synbiohub.org/public/igem/BBa_K1486009/annotation2420779/range2420779/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1486009/annotation2420779/range2420779/1
type: Range
persistent identity: range2420779
display id: range2420779
version: 1
direction: direction/reverse
top level: BBa_K1486009
start: 2398
end: 2409
orientation: inline

BBa_K1486004
type: SequenceAnnotation
persistent identity: annotation2420772
display id: annotation2420772
version: 1
top level: BBa_K1486009
location: https://synbiohub.org/public/igem/BBa_K1486009/annotation2420772/range2420772/1
component: BBa_K1486004

https://synbiohub.org/public/igem/BBa_K1486009/annotation2420772/range2420772/1
type: Range
persistent identity: range2420772
display id: range2420772
version: 1
direction: direction/reverse
top level: BBa_K1486009
start: 1628
end: 1657
orientation: inline

BBa_K1486000
type: SequenceAnnotation
persistent identity: annotation2420766
display id: annotation2420766
version: 1
top level: BBa_K1486009
location: https://synbiohub.org/public/igem/BBa_K1486009/annotation2420766/range2420766/1
component: BBa_K1486000

https://synbiohub.org/public/igem/BBa_K1486009/annotation2420766/range2420766/1
type: Range
persistent identity: range2420766
display id: range2420766
version: 1
direction: direction/reverse
top level: BBa_K1486009
start: 3025
end: 3726
orientation: inline

BBa_K1486006
type: SequenceAnnotation
persistent identity: annotation2420771
display id: annotation2420771
version: 1
top level: BBa_K1486009
location: https://synbiohub.org/public/igem/BBa_K1486009/annotation2420771/range2420771/1
component: BBa_K1486006

https://synbiohub.org/public/igem/BBa_K1486009/annotation2420771/range2420771/1
type: Range
persistent identity: range2420771
display id: range2420771
version: 1
direction: direction/reverse
top level: BBa_K1486009
start: 1223
end: 1627
orientation: inline

BBa_K1486007
type: SequenceAnnotation
persistent identity: annotation2420756
display id: annotation2420756
version: 1
top level: BBa_K1486009
location: https://synbiohub.org/public/igem/BBa_K1486009/annotation2420756/range2420756/1
component: BBa_K1486007

https://synbiohub.org/public/igem/BBa_K1486009/annotation2420756/range2420756/1
type: Range
persistent identity: range2420756
display id: range2420756
version: 1
direction: direction/reverse
top level: BBa_K1486009
start: 2410
end: 2994
orientation: inline

BBa_K1486000
type: SequenceAnnotation
persistent identity: annotation2420788
display id: annotation2420788
version: 1
top level: BBa_K1486009
location: https://synbiohub.org/public/igem/BBa_K1486009/annotation2420788/range2420788/1
component: BBa_K1486000

https://synbiohub.org/public/igem/BBa_K1486009/annotation2420788/range2420788/1
type: Range
persistent identity: range2420788
display id: range2420788
version: 1
direction: direction/reverse
top level: BBa_K1486009
start: 3025
end: 3726
orientation: inline

BBa_K1486052
type: SequenceAnnotation
persistent identity: annotation2420777
display id: annotation2420777
version: 1
top level: BBa_K1486009
location: https://synbiohub.org/public/igem/BBa_K1486009/annotation2420777/range2420777/1
component: BBa_K1486052

https://synbiohub.org/public/igem/BBa_K1486009/annotation2420777/range2420777/1
type: Range
persistent identity: range2420777
display id: range2420777
version: 1
direction: direction/reverse
top level: BBa_K1486009
start: 2360
end: 2397
orientation: inline

BBa_K1486007
type: SequenceAnnotation
persistent identity: annotation2420783
display id: annotation2420783
version: 1
top level: BBa_K1486009
location: https://synbiohub.org/public/igem/BBa_K1486009/annotation2420783/range2420783/1
component: BBa_K1486007

https://synbiohub.org/public/igem/BBa_K1486009/annotation2420783/range2420783/1
type: Range
persistent identity: range2420783
display id: range2420783
version: 1
direction: direction/reverse
top level: BBa_K1486009
start: 2410
end: 2994
orientation: inline

BBa_K1486004
type: SequenceAnnotation
persistent identity: annotation2420784
display id: annotation2420784
version: 1
top level: BBa_K1486009
location: https://synbiohub.org/public/igem/BBa_K1486009/annotation2420784/range2420784/1
component: BBa_K1486004

https://synbiohub.org/public/igem/BBa_K1486009/annotation2420784/range2420784/1
type: Range
persistent identity: range2420784
display id: range2420784
version: 1
direction: direction/reverse
top level: BBa_K1486009
start: 2995
end: 3024
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2420767
display id: annotation2420767
version: 1
top level: BBa_K1486009
location: https://synbiohub.org/public/igem/BBa_K1486009/annotation2420767/range2420767/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1486009/annotation2420767/range2420767/1
type: Range
persistent identity: range2420767
display id: range2420767
version: 1
direction: direction/reverse
top level: BBa_K1486009
start: 1211
end: 1222
orientation: inline

BBa_I0500
type: SequenceAnnotation
persistent identity: annotation2420763
display id: annotation2420763
version: 1
top level: BBa_K1486009
location: https://synbiohub.org/public/igem/BBa_K1486009/annotation2420763/range2420763/1
component: BBa_I0500

https://synbiohub.org/public/igem/BBa_K1486009/annotation2420763/range2420763/1
type: Range
persistent identity: range2420763
display id: range2420763
version: 1
direction: direction/reverse
top level: BBa_K1486009
start: 1
end: 1210
orientation: inline

BBa_K1486000
type: SequenceAnnotation
persistent identity: annotation2420776
display id: annotation2420776
version: 1
top level: BBa_K1486009
location: https://synbiohub.org/public/igem/BBa_K1486009/annotation2420776/range2420776/1
component: BBa_K1486000

https://synbiohub.org/public/igem/BBa_K1486009/annotation2420776/range2420776/1
type: Range
persistent identity: range2420776
display id: range2420776
version: 1
direction: direction/reverse
top level: BBa_K1486009
start: 1658
end: 2359
orientation: inline

BBa_K1486004
type: SequenceAnnotation
persistent identity: annotation2420758
display id: annotation2420758
version: 1
top level: BBa_K1486009
location: https://synbiohub.org/public/igem/BBa_K1486009/annotation2420758/range2420758/1
component: BBa_K1486004

https://synbiohub.org/public/igem/BBa_K1486009/annotation2420758/range2420758/1
type: Range
persistent identity: range2420758
display id: range2420758
version: 1
direction: direction/reverse
top level: BBa_K1486009
start: 2995
end: 3024
orientation: inline

BBa_K678038
type: ComponentDefinition
persistent identity: BBa K678038
display id: BBa_K678038
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: T2-motni, terminator for Aspergillus niger
creator: DTU-Denmark-2
created: 2011-09-03T11:00:00Z
modified: 2015-05-08T01:13:04Z
discontinued: false
dominant: false
experience: experience/None
group u list: _882_
m user id: 0
owner id: 8341
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: T2-motni is a terminator for use in Aspergillus niger
mutable notes: Compatible with the Plug'n'Play assembly standard
mutable provenance: Aspergillus niger genomic DNA
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: T2-motni terminator
sequence annotation: Linker 4
sequence: https://synbiohub.org/public/igem/BBa_K678038_sequence/1

T2-motni terminator
type: SequenceAnnotation
persistent identity: annotation2127408
display id: annotation2127408
version: 1
top level: BBa_K678038
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K678038/annotation2127408/range2127408/1

https://synbiohub.org/public/igem/BBa_K678038/annotation2127408/range2127408/1
type: Range
persistent identity: range2127408
display id: range2127408
version: 1
direction: direction/forward
top level: BBa_K678038
start: 9
end: 990
orientation: inline

Linker 4
type: SequenceAnnotation
persistent identity: annotation2127407
display id: annotation2127407
version: 1
top level: BBa_K678038
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K678038/annotation2127407/range2127407/1

https://synbiohub.org/public/igem/BBa_K678038/annotation2127407/range2127407/1
type: Range
persistent identity: range2127407
display id: range2127407
version: 1
direction: direction/forward
top level: BBa_K678038
start: 1
end: 8
orientation: inline

bleR
type: ComponentDefinition
persistent identity: BBa K678044
display id: BBa_K678044
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: bleR cassette, selection marker for Aspergillus nidulans
creator: DTU-Denmark-2
created: 2011-09-04T11:00:00Z
modified: 2015-05-08T01:13:04Z
discontinued: false
dominant: false
experience: experience/None
group u list: _882_
m user id: 0
owner id: 8341
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: bleR cassette, antibiotic selection marker for Aspergillus nidulans
mutable notes: Compatible with the Plug'n'Play assembly standard
mutable provenance: pAN8-1
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Linker 5
sequence annotation: bleR cassette
sequence: https://synbiohub.org/public/igem/BBa_K678044_sequence/1

Linker 5
type: SequenceAnnotation
persistent identity: annotation2127419
display id: annotation2127419
version: 1
top level: bleR
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K678044/annotation2127419/range2127419/1

https://synbiohub.org/public/igem/BBa_K678044/annotation2127419/range2127419/1
type: Range
persistent identity: range2127419
display id: range2127419
version: 1
direction: direction/forward
top level: bleR
start: 1
end: 8
orientation: inline

bleR cassette
type: SequenceAnnotation
persistent identity: annotation2127420
display id: annotation2127420
version: 1
top level: bleR
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K678044/annotation2127420/range2127420/1

https://synbiohub.org/public/igem/BBa_K678044/annotation2127420/range2127420/1
type: Range
persistent identity: range2127420
display id: range2127420
version: 1
direction: direction/forward
top level: bleR
start: 9
end: 1663
orientation: inline

BBa_B0073
type: ComponentDefinition
persistent identity: BBa B0073
display id: BBa_B0073
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Specialized RBS
creator: Bartholomew Canton
created: 2006-05-01T11:00:00Z
modified: 2015-08-31T04:07:21Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_6_
m user id: 0
owner id: 135
owning group id: 6
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_B0073_sequence/1

pJEJAM15
type: ComponentDefinition
persistent identity: BBa K678063
display id: BBa_K678063
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pJEJAM15
creator: DTU-Denmark-2
created: 2011-09-12T11:00:00Z
modified: 2015-05-08T01:13:05Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _882_
m user id: 0
owner id: 8341
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: To be announced
mutable notes: To be announced
mutable provenance: To be announced
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000155
role: partType/Plasmid
component: BBa_K678034
component: BBa_K678036
component: BBa_K678040
component: BBa_K678046
component: BBa_K678024
sequence annotation: BBa_K678040
sequence annotation: BBa_K678036
sequence annotation: BBa_K678046
sequence annotation: BBa_K678024
sequence annotation: BBa_K678034
sequence: https://synbiohub.org/public/igem/BBa_K678063_sequence/1

BBa_K678034
type: Component
persistent identity: component2135949
display id: component2135949
version: 1
top level: pJEJAM15
access: public
definition: mRFP1-NLS

BBa_K678036
type: Component
persistent identity: component2135952
display id: component2135952
version: 1
top level: pJEJAM15
access: public
definition: TtrpC

BBa_K678040
type: Component
persistent identity: component2135955
display id: component2135955
version: 1
top level: pJEJAM15
access: public
definition: pyrG casse

BBa_K678046
type: Component
persistent identity: component2135958
display id: component2135958
version: 1
top level: pJEJAM15
access: public
definition: pFun

BBa_K678024
type: Component
persistent identity: component2135943
display id: component2135943
version: 1
top level: pJEJAM15
access: public
definition: PgpdA

BBa_K678040
type: SequenceAnnotation
persistent identity: annotation2135955
display id: annotation2135955
version: 1
top level: pJEJAM15
location: https://synbiohub.org/public/igem/BBa_K678063/annotation2135955/range2135955/1
component: BBa_K678040

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type: Range
persistent identity: range2135955
display id: range2135955
version: 1
direction: direction/reverse
top level: pJEJAM15
start: 3779
end: 5234
orientation: inline

BBa_K678036
type: SequenceAnnotation
persistent identity: annotation2135952
display id: annotation2135952
version: 1
top level: pJEJAM15
location: https://synbiohub.org/public/igem/BBa_K678063/annotation2135952/range2135952/1
component: BBa_K678036

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type: Range
persistent identity: range2135952
display id: range2135952
version: 1
direction: direction/reverse
top level: pJEJAM15
start: 3020
end: 3778
orientation: inline

BBa_K678046
type: SequenceAnnotation
persistent identity: annotation2135958
display id: annotation2135958
version: 1
top level: pJEJAM15
location: https://synbiohub.org/public/igem/BBa_K678063/annotation2135958/range2135958/1
component: BBa_K678046

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type: Range
persistent identity: range2135958
display id: range2135958
version: 1
direction: direction/reverse
top level: pJEJAM15
start: 5235
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orientation: inline

BBa_K678024
type: SequenceAnnotation
persistent identity: annotation2135943
display id: annotation2135943
version: 1
top level: pJEJAM15
location: https://synbiohub.org/public/igem/BBa_K678063/annotation2135943/range2135943/1
component: BBa_K678024

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type: Range
persistent identity: range2135943
display id: range2135943
version: 1
direction: direction/reverse
top level: pJEJAM15
start: 1
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orientation: inline

BBa_K678034
type: SequenceAnnotation
persistent identity: annotation2135949
display id: annotation2135949
version: 1
top level: pJEJAM15
location: https://synbiohub.org/public/igem/BBa_K678063/annotation2135949/range2135949/1
component: BBa_K678034

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type: Range
persistent identity: range2135949
display id: range2135949
version: 1
direction: direction/reverse
top level: pJEJAM15
start: 2313
end: 3019
orientation: inline

BBa_K1520516
type: ComponentDefinition
persistent identity: BBa K1520516
display id: BBa_K1520516
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: MC31-rbs-luxI-Ter-Pcons2-rbs-luxR-Ter-Prlux-rbs-OMPA-golB-rbs-luxI-Ter
creator: yue wu
created: 2014-09-22T11:00:00Z
modified: 2015-05-08T01:10:49Z
discontinued: false
dominant: false
group u list: _1902_
m user id: 0
owner id: 21318
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: MC31-rbs-luxI-Ter-Pcons2-rbs-luxR-Ter-Prlux-rbs-OMPA-golB-rbs-luxI-Ter It is a test of our positive feedback circuit for microcystin.
mutable notes: None
mutable provenance: MC31, luxI, Pcons2, luxR, Prlux, OMPA-golB, Ter
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1520002
component: BBa_K1520507
component: BBa_K1520504
component: BBa_K1520029
component: BBa_K1520003
component: BBa_R0062
sequence annotation: BBa_K1520029
sequence annotation: BBa_K1520002
sequence annotation: BBa_K1520504
sequence annotation: BBa_R0062
sequence annotation: BBa_K1520507
sequence annotation: BBa_K1520003
sequence: https://synbiohub.org/public/igem/BBa_K1520516_sequence/1

BBa_K1520002
type: Component
persistent identity: component2385692
display id: component2385692
version: 1
top level: BBa_K1520516
access: public
definition: https://synbiohub.org/public/igem/BBa_K1520002/1

BBa_K1520507
type: Component
persistent identity: component2385714
display id: component2385714
version: 1
top level: BBa_K1520516
access: public
definition: https://synbiohub.org/public/igem/BBa_K1520507/1

BBa_K1520504
type: Component
persistent identity: component2385717
display id: component2385717
version: 1
top level: BBa_K1520516
access: public
definition: https://synbiohub.org/public/igem/BBa_K1520504/1

BBa_K1520029
type: Component
persistent identity: component2385718
display id: component2385718
version: 1
top level: BBa_K1520516
access: public
definition: https://synbiohub.org/public/igem/BBa_K1520029/1

BBa_K1520003
type: Component
persistent identity: component2385702
display id: component2385702
version: 1
top level: BBa_K1520516
access: public
definition: https://synbiohub.org/public/igem/BBa_K1520003/1

BBa_K1520029
type: Component
persistent identity: component2385696
display id: component2385696
version: 1
top level: BBa_K1520516
access: public
definition: https://synbiohub.org/public/igem/BBa_K1520029/1

BBa_R0062
type: Component
persistent identity: component2385704
display id: component2385704
version: 1
top level: BBa_K1520516
access: public
definition: lux pR

BBa_K1520504
type: Component
persistent identity: component2385695
display id: component2385695
version: 1
top level: BBa_K1520516
access: public
definition: https://synbiohub.org/public/igem/BBa_K1520504/1

BBa_K1520029
type: SequenceAnnotation
persistent identity: annotation2385718
display id: annotation2385718
version: 1
top level: BBa_K1520516
location: https://synbiohub.org/public/igem/BBa_K1520516/annotation2385718/range2385718/1
component: BBa_K1520029

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type: Range
persistent identity: range2385718
display id: range2385718
version: 1
direction: direction/reverse
top level: BBa_K1520516
start: 3482
end: 3610
orientation: inline

BBa_K1520002
type: SequenceAnnotation
persistent identity: annotation2385692
display id: annotation2385692
version: 1
top level: BBa_K1520516
location: https://synbiohub.org/public/igem/BBa_K1520516/annotation2385692/range2385692/1
component: BBa_K1520002

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type: Range
persistent identity: range2385692
display id: range2385692
version: 1
direction: direction/reverse
top level: BBa_K1520516
start: 1
end: 132
orientation: inline

BBa_K1520504
type: SequenceAnnotation
persistent identity: annotation2385717
display id: annotation2385717
version: 1
top level: BBa_K1520516
location: https://synbiohub.org/public/igem/BBa_K1520516/annotation2385717/range2385717/1
component: BBa_K1520504

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type: Range
persistent identity: range2385717
display id: range2385717
version: 1
direction: direction/reverse
top level: BBa_K1520516
start: 2813
end: 3473
orientation: inline

BBa_R0062
type: SequenceAnnotation
persistent identity: annotation2385704
display id: annotation2385704
version: 1
top level: BBa_K1520516
location: https://synbiohub.org/public/igem/BBa_K1520516/annotation2385704/range2385704/1
component: BBa_R0062

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type: Range
persistent identity: range2385704
display id: range2385704
version: 1
direction: direction/forward
top level: BBa_K1520516
start: 1934
end: 1988
orientation: inline

BBa_K1520504
type: SequenceAnnotation
persistent identity: annotation2385695
display id: annotation2385695
version: 1
top level: BBa_K1520516
location: https://synbiohub.org/public/igem/BBa_K1520516/annotation2385695/range2385695/1
component: BBa_K1520504

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type: Range
persistent identity: range2385695
display id: range2385695
version: 1
direction: direction/reverse
top level: BBa_K1520516
start: 141
end: 801
orientation: inline

BBa_K1520507
type: SequenceAnnotation
persistent identity: annotation2385714
display id: annotation2385714
version: 1
top level: BBa_K1520516
location: https://synbiohub.org/public/igem/BBa_K1520516/annotation2385714/range2385714/1
component: BBa_K1520507

https://synbiohub.org/public/igem/BBa_K1520516/annotation2385714/range2385714/1
type: Range
persistent identity: range2385714
display id: range2385714
version: 1
direction: direction/reverse
top level: BBa_K1520516
start: 1995
end: 2804
orientation: inline

BBa_K1520003
type: SequenceAnnotation
persistent identity: annotation2385702
display id: annotation2385702
version: 1
top level: BBa_K1520516
location: https://synbiohub.org/public/igem/BBa_K1520516/annotation2385702/range2385702/1
component: BBa_K1520003

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type: Range
persistent identity: range2385702
display id: range2385702
version: 1
direction: direction/reverse
top level: BBa_K1520516
start: 947
end: 1925
orientation: inline

BBa_K1520029
type: SequenceAnnotation
persistent identity: annotation2385696
display id: annotation2385696
version: 1
top level: BBa_K1520516
location: https://synbiohub.org/public/igem/BBa_K1520516/annotation2385696/range2385696/1
component: BBa_K1520029

https://synbiohub.org/public/igem/BBa_K1520516/annotation2385696/range2385696/1
type: Range
persistent identity: range2385696
display id: range2385696
version: 1
direction: direction/reverse
top level: BBa_K1520516
start: 810
end: 938
orientation: inline

BBa_K678004
type: ComponentDefinition
persistent identity: BBa K678004
display id: BBa_K678004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PGK, mammalian promoter
creator: DTU-Denmark-2
created: 2011-09-03T11:00:00Z
modified: 2015-05-08T01:13:04Z
discontinued: false
dominant: false
experience: experience/None
group u list: _882_
m user id: 0
owner id: 8341
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Promoter for expression of genes in mammalian cells
mutable notes: Forward direction
mutable provenance: From plasmid pSuperior.puro from ￼￼pSuper RNAi System, OligoEngine
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: Linker 1
sequence annotation: PGK
sequence: https://synbiohub.org/public/igem/BBa_K678004_sequence/1

Linker 1
type: SequenceAnnotation
persistent identity: annotation2127344
display id: annotation2127344
version: 1
top level: BBa_K678004
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K678004/annotation2127344/range2127344/1

https://synbiohub.org/public/igem/BBa_K678004/annotation2127344/range2127344/1
type: Range
persistent identity: range2127344
display id: range2127344
version: 1
direction: direction/forward
top level: BBa_K678004
start: 1
end: 8
orientation: inline

PGK
type: SequenceAnnotation
persistent identity: annotation2127345
display id: annotation2127345
version: 1
top level: BBa_K678004
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K678004/annotation2127345/range2127345/1

https://synbiohub.org/public/igem/BBa_K678004/annotation2127345/range2127345/1
type: Range
persistent identity: range2127345
display id: range2127345
version: 1
direction: direction/forward
top level: BBa_K678004
start: 9
end: 407
orientation: inline

BBa_J61145
type: ComponentDefinition
persistent identity: BBa J61145
display id: BBa_J61145
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: {rbs1} Library Member in BBb
creator: John Anderson
created: 2007-09-03T11:00:00Z
modified: 2015-08-31T01:56:25Z
discontinued: false
dominant: false
experience: experience/None
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: {{BBbRBS1Family}}
mutable notes: {{BerkiGEM2007-Biobrick2.0Intro}}
mutable provenance: Synthetic
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J61145_sequence/1

BBa_K1486030
type: ComponentDefinition
persistent identity: BBa K1486030
display id: BBa_K1486030
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: rLucN + ADH1 terminator + Kanamycin resistance
creator: EPFL iGem team 2014
created: 2014-09-26T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: To edit
mutable notes: To edit
mutable provenance: To edit
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K1486019
component: BBa_K1486025
component: BBa_K1486037
component: BBa_K1486024
sequence annotation: BBa_K1486025
sequence annotation: BBa_K1486024
sequence annotation: BBa_K1486019
sequence annotation: BBa_K1486037
sequence: https://synbiohub.org/public/igem/BBa_K1486030_sequence/1

BBa_K1486019
type: Component
persistent identity: component2417717
display id: component2417717
version: 1
top level: BBa_K1486030
access: public
definition: BBa_K1486019

BBa_K1486025
type: Component
persistent identity: component2417718
display id: component2417718
version: 1
top level: BBa_K1486030
access: public
definition: BBa_K1486025

BBa_K1486037
type: Component
persistent identity: component2417716
display id: component2417716
version: 1
top level: BBa_K1486030
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486037/1

BBa_K1486024
type: Component
persistent identity: component2417722
display id: component2417722
version: 1
top level: BBa_K1486030
access: public
definition: BBa_K1486024

BBa_K1486025
type: SequenceAnnotation
persistent identity: annotation2417718
display id: annotation2417718
version: 1
top level: BBa_K1486030
location: https://synbiohub.org/public/igem/BBa_K1486030/annotation2417718/range2417718/1
component: BBa_K1486025

https://synbiohub.org/public/igem/BBa_K1486030/annotation2417718/range2417718/1
type: Range
persistent identity: range2417718
display id: range2417718
version: 1
direction: direction/reverse
top level: BBa_K1486030
start: 370
end: 557
orientation: inline

BBa_K1486024
type: SequenceAnnotation
persistent identity: annotation2417722
display id: annotation2417722
version: 1
top level: BBa_K1486030
location: https://synbiohub.org/public/igem/BBa_K1486030/annotation2417722/range2417722/1
component: BBa_K1486024

https://synbiohub.org/public/igem/BBa_K1486030/annotation2417722/range2417722/1
type: Range
persistent identity: range2417722
display id: range2417722
version: 1
direction: direction/reverse
top level: BBa_K1486030
start: 558
end: 1914
orientation: inline

BBa_K1486019
type: SequenceAnnotation
persistent identity: annotation2417717
display id: annotation2417717
version: 1
top level: BBa_K1486030
location: https://synbiohub.org/public/igem/BBa_K1486030/annotation2417717/range2417717/1
component: BBa_K1486019

https://synbiohub.org/public/igem/BBa_K1486030/annotation2417717/range2417717/1
type: Range
persistent identity: range2417717
display id: range2417717
version: 1
direction: direction/reverse
top level: BBa_K1486030
start: 40
end: 369
orientation: inline

BBa_K1486037
type: SequenceAnnotation
persistent identity: annotation2417716
display id: annotation2417716
version: 1
top level: BBa_K1486030
location: https://synbiohub.org/public/igem/BBa_K1486030/annotation2417716/range2417716/1
component: BBa_K1486037

https://synbiohub.org/public/igem/BBa_K1486030/annotation2417716/range2417716/1
type: Range
persistent identity: range2417716
display id: range2417716
version: 1
direction: direction/reverse
top level: BBa_K1486030
start: 1
end: 39
orientation: inline

BBa_J64065
type: ComponentDefinition
persistent identity: BBa J64065
display id: BBa_J64065
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: cI repressed promoter
creator: Jeffrey J Tabor
created: 2007-05-12T11:00:00Z
modified: 2015-05-08T01:08:16Z
discontinued: false
dominant: false
experience: experience/Fails
group u list: _98_
m user id: 0
owner id: 88
owning group id: 98
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is a LuxR-30C6HSL-independent version of R0065. All DNA upstream of the -10 box is from the constitutive promoter J23119. All DNA downstream from the -10 box down is from R0065. This results in the replacement of the Lux box region for a constitutive promoter region and the maintenance of the two cI operators downstream of the transcription start site.
mutable notes: none
mutable provenance: J23119 + R0065
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: +1
sequence annotation: OR1
sequence annotation: -35
sequence annotation: OR2
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_J64065_sequence/1

+1
type: SequenceAnnotation
persistent identity: annotation1932685
display id: annotation1932685
version: 1
top level: BBa_J64065
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_J64065/annotation1932685/range1932685/1

https://synbiohub.org/public/igem/BBa_J64065/annotation1932685/range1932685/1
type: Range
persistent identity: range1932685
display id: range1932685
version: 1
direction: direction/forward
top level: BBa_J64065
start: 35
end: 35
orientation: inline

OR1
type: SequenceAnnotation
persistent identity: annotation1932683
display id: annotation1932683
version: 1
top level: BBa_J64065
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_J64065/annotation1932683/range1932683/1

https://synbiohub.org/public/igem/BBa_J64065/annotation1932683/range1932683/1
type: Range
persistent identity: range1932683
display id: range1932683
version: 1
direction: direction/forward
top level: BBa_J64065
start: 34
end: 51
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1932681
display id: annotation1932681
version: 1
top level: BBa_J64065
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_J64065/annotation1932681/range1932681/1

https://synbiohub.org/public/igem/BBa_J64065/annotation1932681/range1932681/1
type: Range
persistent identity: range1932681
display id: range1932681
version: 1
direction: direction/forward
top level: BBa_J64065
start: 1
end: 6
orientation: inline

OR2
type: SequenceAnnotation
persistent identity: annotation1932684
display id: annotation1932684
version: 1
top level: BBa_J64065
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_J64065/annotation1932684/range1932684/1

https://synbiohub.org/public/igem/BBa_J64065/annotation1932684/range1932684/1
type: Range
persistent identity: range1932684
display id: range1932684
version: 1
direction: direction/forward
top level: BBa_J64065
start: 58
end: 74
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1932682
display id: annotation1932682
version: 1
top level: BBa_J64065
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_J64065/annotation1932682/range1932682/1

https://synbiohub.org/public/igem/BBa_J64065/annotation1932682/range1932682/1
type: Range
persistent identity: range1932682
display id: range1932682
version: 1
direction: direction/forward
top level: BBa_J64065
start: 24
end: 29
orientation: inline

pJEJAM14
type: ComponentDefinition
persistent identity: BBa K678062
display id: BBa_K678062
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pJEJAM14
creator: DTU-Denmark-2
created: 2011-09-12T11:00:00Z
modified: 2015-05-08T01:13:05Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _882_
m user id: 0
owner id: 8341
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: To be announced
mutable notes: To be announced
mutable provenance: To be announced
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000155
role: partType/Plasmid
component: BBa_K678030
component: BBa_K678036
component: BBa_K678046
component: BBa_K678040
component: BBa_K678024
sequence annotation: BBa_K678024
sequence annotation: BBa_K678046
sequence annotation: BBa_K678030
sequence annotation: BBa_K678040
sequence annotation: BBa_K678036
sequence: https://synbiohub.org/public/igem/BBa_K678062_sequence/1

BBa_K678030
type: Component
persistent identity: component2135931
display id: component2135931
version: 1
top level: pJEJAM14
access: public
definition: mRFP1

BBa_K678036
type: Component
persistent identity: component2135934
display id: component2135934
version: 1
top level: pJEJAM14
access: public
definition: TtrpC

BBa_K678046
type: Component
persistent identity: component2135940
display id: component2135940
version: 1
top level: pJEJAM14
access: public
definition: pFun

BBa_K678040
type: Component
persistent identity: component2135937
display id: component2135937
version: 1
top level: pJEJAM14
access: public
definition: pyrG casse

BBa_K678024
type: Component
persistent identity: component2135928
display id: component2135928
version: 1
top level: pJEJAM14
access: public
definition: PgpdA

BBa_K678024
type: SequenceAnnotation
persistent identity: annotation2135928
display id: annotation2135928
version: 1
top level: pJEJAM14
location: https://synbiohub.org/public/igem/BBa_K678062/annotation2135928/range2135928/1
component: BBa_K678024

https://synbiohub.org/public/igem/BBa_K678062/annotation2135928/range2135928/1
type: Range
persistent identity: range2135928
display id: range2135928
version: 1
direction: direction/reverse
top level: pJEJAM14
start: 1
end: 2312
orientation: inline

BBa_K678046
type: SequenceAnnotation
persistent identity: annotation2135940
display id: annotation2135940
version: 1
top level: pJEJAM14
location: https://synbiohub.org/public/igem/BBa_K678062/annotation2135940/range2135940/1
component: BBa_K678046

https://synbiohub.org/public/igem/BBa_K678062/annotation2135940/range2135940/1
type: Range
persistent identity: range2135940
display id: range2135940
version: 1
direction: direction/reverse
top level: pJEJAM14
start: 5214
end: 8091
orientation: inline

BBa_K678030
type: SequenceAnnotation
persistent identity: annotation2135931
display id: annotation2135931
version: 1
top level: pJEJAM14
location: https://synbiohub.org/public/igem/BBa_K678062/annotation2135931/range2135931/1
component: BBa_K678030

https://synbiohub.org/public/igem/BBa_K678062/annotation2135931/range2135931/1
type: Range
persistent identity: range2135931
display id: range2135931
version: 1
direction: direction/reverse
top level: pJEJAM14
start: 2313
end: 2998
orientation: inline

BBa_K678040
type: SequenceAnnotation
persistent identity: annotation2135937
display id: annotation2135937
version: 1
top level: pJEJAM14
location: https://synbiohub.org/public/igem/BBa_K678062/annotation2135937/range2135937/1
component: BBa_K678040

https://synbiohub.org/public/igem/BBa_K678062/annotation2135937/range2135937/1
type: Range
persistent identity: range2135937
display id: range2135937
version: 1
direction: direction/reverse
top level: pJEJAM14
start: 3758
end: 5213
orientation: inline

BBa_K678036
type: SequenceAnnotation
persistent identity: annotation2135934
display id: annotation2135934
version: 1
top level: pJEJAM14
location: https://synbiohub.org/public/igem/BBa_K678062/annotation2135934/range2135934/1
component: BBa_K678036

https://synbiohub.org/public/igem/BBa_K678062/annotation2135934/range2135934/1
type: Range
persistent identity: range2135934
display id: range2135934
version: 1
direction: direction/reverse
top level: pJEJAM14
start: 2999
end: 3757
orientation: inline

BioScaffol
type: ComponentDefinition
persistent identity: BBa K259004
display id: BBa_K259004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: BioScaffold Linker - Removes Stop Codons & scars & replaces with a Gly-Ser linker
creator: Petros Mina
created: 2009-07-31T11:00:00Z
modified: 2015-05-08T01:11:42Z
discontinued: false
dominant: false
experience: experience/None
group u list: _357_
m user id: 0
owner id: 5318
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part is part of a family of Bioscaffold - Linkers. This part will help users interested in protein-fusion. The usage of the part will allow in frame assembly of 2(+) proteins by: *Removing the stop codons from the upstream protein. *Removing the scar created by the Assembly Standard 10 (RFC10) construction of 2(+) protein coding sequences. *Maintaining the start codon of the downstream protein. *Adding a flexible (Gly-Ser-Gly) linker between the two protein coding sequences. *Acting as a translational buffer if ligation is incorrect. ===Assembly Instructions=== *Create an Assembly Standard 10 fusion as below ; **Upstream Protein Part - Bioscaffold Linker - Downstream Protein Part** *Use the BioScaffold Specific Enzymes (i)Restrict with BpuEI to remove upstream scar (created between Upstream Protein - Bioscaffold) and stop codons from upstream protein part. (ii)Ligate and you will get the intermediate product; **Upstream Protein (w/out Stop codons) - Tyr - BioScaffold Linker - Downstream Protein** *Restrict with CspCI to remove downstream scar. *Ligate to get the final product; **Upstream Protein - Tyr-Gly-Ser-Gly- Downstream Protein**
mutable notes: This part was designed taking into consideration the requirements by Assembly Standard 10 (RFC10).
mutable provenance: This part is completely designed ''de novo''. This sequence does not occur naturally.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: BpuEI
sequence annotation: Translational Buffer
sequence annotation: CspCI (3' Recognition Seq)
sequence annotation: Met Involved in Restriction/Ligation
sequence annotation: Gly-Ser-Gly Linker
sequence annotation: CspCI (5' Recognition Seq)
sequence annotation: Tyr Involved in Restriction/Ligation
sequence: https://synbiohub.org/public/igem/BBa_K259004_sequence/1

BpuEI
type: SequenceAnnotation
persistent identity: annotation2014598
display id: annotation2014598
version: 1
top level: BioScaffol
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K259004/annotation2014598/range2014598/1

https://synbiohub.org/public/igem/BBa_K259004/annotation2014598/range2014598/1
type: Range
persistent identity: range2014598
display id: range2014598
version: 1
direction: direction/forward
top level: BioScaffol
start: 21
end: 26
orientation: inline

BpuEI
type: SequenceAnnotation
persistent identity: annotation2014597
display id: annotation2014597
version: 1
top level: BioScaffol
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K259004/annotation2014597/range2014597/1

https://synbiohub.org/public/igem/BBa_K259004/annotation2014597/range2014597/1
type: Range
persistent identity: range2014597
display id: range2014597
version: 1
direction: direction/reverse
top level: BioScaffol
start: 3
end: 8
orientation: inline

Translational Buffer
type: SequenceAnnotation
persistent identity: annotation2014602
display id: annotation2014602
version: 1
top level: BioScaffol
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K259004/annotation2014602/range2014602/1

https://synbiohub.org/public/igem/BBa_K259004/annotation2014602/range2014602/1
type: Range
persistent identity: range2014602
display id: range2014602
version: 1
direction: direction/forward
top level: BioScaffol
start: 56
end: 65
orientation: inline

CspCI (3' Recognition Seq)
type: SequenceAnnotation
persistent identity: annotation2014605
display id: annotation2014605
version: 1
top level: BioScaffol
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K259004/annotation2014605/range2014605/1

https://synbiohub.org/public/igem/BBa_K259004/annotation2014605/range2014605/1
type: Range
persistent identity: range2014605
display id: range2014605
version: 1
direction: direction/forward
top level: BioScaffol
start: 74
end: 77
orientation: inline

Met Involved in Restriction/Ligation
type: SequenceAnnotation
persistent identity: annotation2014601
display id: annotation2014601
version: 1
top level: BioScaffol
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K259004/annotation2014601/range2014601/1

https://synbiohub.org/public/igem/BBa_K259004/annotation2014601/range2014601/1
type: Range
persistent identity: range2014601
display id: range2014601
version: 1
direction: direction/forward
top level: BioScaffol
start: 53
end: 55
orientation: inline

Gly-Ser-Gly Linker
type: SequenceAnnotation
persistent identity: annotation2014600
display id: annotation2014600
version: 1
top level: BioScaffol
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K259004/annotation2014600/range2014600/1

https://synbiohub.org/public/igem/BBa_K259004/annotation2014600/range2014600/1
type: Range
persistent identity: range2014600
display id: range2014600
version: 1
direction: direction/forward
top level: BioScaffol
start: 44
end: 52
orientation: inline

Translational Buffer
type: SequenceAnnotation
persistent identity: annotation2014604
display id: annotation2014604
version: 1
top level: BioScaffol
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K259004/annotation2014604/range2014604/1

https://synbiohub.org/public/igem/BBa_K259004/annotation2014604/range2014604/1
type: Range
persistent identity: range2014604
display id: range2014604
version: 1
direction: direction/forward
top level: BioScaffol
start: 70
end: 72
orientation: inline

CspCI (5' Recognition Seq)
type: SequenceAnnotation
persistent identity: annotation2014603
display id: annotation2014603
version: 1
top level: BioScaffol
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K259004/annotation2014603/range2014603/1

https://synbiohub.org/public/igem/BBa_K259004/annotation2014603/range2014603/1
type: Range
persistent identity: range2014603
display id: range2014603
version: 1
direction: direction/forward
top level: BioScaffol
start: 66
end: 68
orientation: inline

Tyr Involved in Restriction/Ligation
type: SequenceAnnotation
persistent identity: annotation2014599
display id: annotation2014599
version: 1
top level: BioScaffol
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K259004/annotation2014599/range2014599/1

https://synbiohub.org/public/igem/BBa_K259004/annotation2014599/range2014599/1
type: Range
persistent identity: range2014599
display id: range2014599
version: 1
direction: direction/forward
top level: BioScaffol
start: 41
end: 43
orientation: inline

BBa_I732276
type: ComponentDefinition
persistent identity: BBa I732276
display id: BBa_I732276
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Family Member with Hybrid Operator (D001O27)
creator: Ma Rui
created: 2007-10-13T11:00:00Z
modified: 2015-08-31T04:07:57Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732276_sequence/1

pJEJAM20
type: ComponentDefinition
persistent identity: BBa K678068
display id: BBa_K678068
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pJEJAM20
creator: DTU-Denmark-2
created: 2011-09-12T11:00:00Z
modified: 2015-05-08T01:13:05Z
discontinued: false
dominant: false
experience: experience/None
group u list: _882_
m user id: 0
owner id: 8341
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: To be announced
mutable notes: To be announced
mutable provenance: To be announced
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000155
role: partType/Plasmid
component: BBa_K678046
component: BBa_K678047
component: BBa_K678032
component: BBa_K678036
component: BBa_K678001
component: BBa_K678035
sequence annotation: BBa_K678001
sequence annotation: BBa_K678032
sequence annotation: BBa_K678036
sequence annotation: BBa_K678035
sequence annotation: BBa_K678046
sequence annotation: BBa_K678047
sequence: https://synbiohub.org/public/igem/BBa_K678068_sequence/1

BBa_K678046
type: Component
persistent identity: component2127644
display id: component2127644
version: 1
top level: pJEJAM20
access: public
definition: pFun

BBa_K678047
type: Component
persistent identity: component2127641
display id: component2127641
version: 1
top level: pJEJAM20
access: public
definition: BBa_K678047

BBa_K678032
type: Component
persistent identity: component2127635
display id: component2127635
version: 1
top level: pJEJAM20
access: public
definition: mRFP1

BBa_K678036
type: Component
persistent identity: component2127638
display id: component2127638
version: 1
top level: pJEJAM20
access: public
definition: TtrpC

BBa_K678001
type: Component
persistent identity: component2127629
display id: component2127629
version: 1
top level: pJEJAM20
access: public
definition: PalcA

BBa_K678035
type: Component
persistent identity: component2127632
display id: component2127632
version: 1
top level: pJEJAM20
access: public
definition: yA

BBa_K678001
type: SequenceAnnotation
persistent identity: annotation2127629
display id: annotation2127629
version: 1
top level: pJEJAM20
location: https://synbiohub.org/public/igem/BBa_K678068/annotation2127629/range2127629/1
component: BBa_K678001

https://synbiohub.org/public/igem/BBa_K678068/annotation2127629/range2127629/1
type: Range
persistent identity: range2127629
display id: range2127629
version: 1
direction: direction/reverse
top level: pJEJAM20
start: 1
end: 843
orientation: inline

BBa_K678032
type: SequenceAnnotation
persistent identity: annotation2127635
display id: annotation2127635
version: 1
top level: pJEJAM20
location: https://synbiohub.org/public/igem/BBa_K678068/annotation2127635/range2127635/1
component: BBa_K678032

https://synbiohub.org/public/igem/BBa_K678068/annotation2127635/range2127635/1
type: Range
persistent identity: range2127635
display id: range2127635
version: 1
direction: direction/reverse
top level: pJEJAM20
start: 3016
end: 3702
orientation: inline

BBa_K678036
type: SequenceAnnotation
persistent identity: annotation2127638
display id: annotation2127638
version: 1
top level: pJEJAM20
location: https://synbiohub.org/public/igem/BBa_K678068/annotation2127638/range2127638/1
component: BBa_K678036

https://synbiohub.org/public/igem/BBa_K678068/annotation2127638/range2127638/1
type: Range
persistent identity: range2127638
display id: range2127638
version: 1
direction: direction/reverse
top level: pJEJAM20
start: 3711
end: 4469
orientation: inline

BBa_K678035
type: SequenceAnnotation
persistent identity: annotation2127632
display id: annotation2127632
version: 1
top level: pJEJAM20
location: https://synbiohub.org/public/igem/BBa_K678068/annotation2127632/range2127632/1
component: BBa_K678035

https://synbiohub.org/public/igem/BBa_K678068/annotation2127632/range2127632/1
type: Range
persistent identity: range2127632
display id: range2127632
version: 1
direction: direction/reverse
top level: pJEJAM20
start: 852
end: 3007
orientation: inline

BBa_K678046
type: SequenceAnnotation
persistent identity: annotation2127644
display id: annotation2127644
version: 1
top level: pJEJAM20
location: https://synbiohub.org/public/igem/BBa_K678068/annotation2127644/range2127644/1
component: BBa_K678046

https://synbiohub.org/public/igem/BBa_K678068/annotation2127644/range2127644/1
type: Range
persistent identity: range2127644
display id: range2127644
version: 1
direction: direction/reverse
top level: pJEJAM20
start: 5353
end: 8230
orientation: inline

BBa_K678047
type: SequenceAnnotation
persistent identity: annotation2127641
display id: annotation2127641
version: 1
top level: pJEJAM20
location: https://synbiohub.org/public/igem/BBa_K678068/annotation2127641/range2127641/1
component: BBa_K678047

https://synbiohub.org/public/igem/BBa_K678068/annotation2127641/range2127641/1
type: Range
persistent identity: range2127641
display id: range2127641
version: 1
direction: direction/reverse
top level: pJEJAM20
start: 4478
end: 5344
orientation: inline

BBa_K108014
type: ComponentDefinition
persistent identity: BBa K108014
display id: BBa_K108014
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PR
creator: Qi Liu
created: 2008-10-22T11:00:00Z
modified: 2015-05-08T01:09:04Z
discontinued: false
dominant: false
experience: experience/None
group u list: _222_
m user id: 0
owner id: 2671
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: promoter of Alcaligenes eutrophus PhaR gene
mutable notes: PCR using primers with biobrick prefix and surfix, cloned into pSB1AC3, and then eliminate Pst1 using site-directed mutation.
mutable provenance: Alcaligenes eutrophus H16 genome
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: PR
sequence: https://synbiohub.org/public/igem/BBa_K108014_sequence/1

PR
type: SequenceAnnotation
persistent identity: annotation1986959
display id: annotation1986959
version: 1
top level: BBa_K108014
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K108014/annotation1986959/range1986959/1

https://synbiohub.org/public/igem/BBa_K108014/annotation1986959/range1986959/1
type: Range
persistent identity: range1986959
display id: range1986959
version: 1
direction: direction/forward
top level: BBa_K108014
start: 1
end: 234
orientation: inline

BBa_R0186
type: ComponentDefinition
persistent identity: BBa R0186
display id: BBa_R0186
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: T7 promoter (lacI repressible)
creator: Bartholomew Canton
created: 2008-04-07T11:00:00Z
modified: 2015-05-08T01:14:15Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_
m user id: 0
owner id: 135
owning group id: 84
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: A lac repressible T7 promoter. This promoter is similar to those found in the pET vectors with T7lac promoters. I mutated the -9 base to an A, which should make it less than 3% of the strength of consensus. The base numbering is as used by Studier and coworkers (JMB 1991, 219, 45-59). See BBa_R0183 for more information.
mutable notes: The -9 mutation from consensus was included to weaken the consensus promoter as described by Imburgio and co-workers (see BBa_R0183 for more information)
mutable provenance: This sequence is based on the T7lac promoter described by Dubendorff and Studier (JMB 1991, 219, 45-59). The only modification is the mutation at -9.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: T7 promoter
sequence annotation: LacI binding site
sequence: https://synbiohub.org/public/igem/BBa_R0186_sequence/1

T7 promoter
type: SequenceAnnotation
persistent identity: annotation1962095
display id: annotation1962095
version: 1
top level: BBa_R0186
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0186/annotation1962095/range1962095/1

https://synbiohub.org/public/igem/BBa_R0186/annotation1962095/range1962095/1
type: Range
persistent identity: range1962095
display id: range1962095
version: 1
direction: direction/forward
top level: BBa_R0186
start: 1
end: 20
orientation: inline

LacI binding site
type: SequenceAnnotation
persistent identity: annotation1962094
display id: annotation1962094
version: 1
top level: BBa_R0186
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0186/annotation1962094/range1962094/1

https://synbiohub.org/public/igem/BBa_R0186/annotation1962094/range1962094/1
type: Range
persistent identity: range1962094
display id: range1962094
version: 1
direction: direction/forward
top level: BBa_R0186
start: 20
end: 44
orientation: inline

las TetO
type: ComponentDefinition
persistent identity: BBa I14015
display id: BBa_I14015
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: P(Las) TetO
creator: Vikram Vijayan, Allen Hsu, Lawrence Fomundam
created: 2004-07-29T11:00:00Z
modified: 2015-08-31T04:07:37Z
discontinued: false
dominant: false
experience: experience/None
group u list: _4_
m user id: 0
owner id: 171
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: LasR and 3OC12HSL regulated promoter with a single Tet Operator (binding site for TetR repressor)
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -35
sequence annotation: R0079
sequence annotation: TetR
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_I14015_sequence/1

-35
type: SequenceAnnotation
persistent identity: annotation1026120
display id: annotation1026120
version: 1
top level: las TetO
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I14015/annotation1026120/range1026120/1

https://synbiohub.org/public/igem/BBa_I14015/annotation1026120/range1026120/1
type: Range
persistent identity: range1026120
display id: range1026120
version: 1
direction: direction/forward
top level: las TetO
start: 117
end: 122
orientation: inline

R0079
type: SequenceAnnotation
persistent identity: annotation1017909
display id: annotation1017909
version: 1
top level: las TetO
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_I14015/annotation1017909/range1017909/1

https://synbiohub.org/public/igem/BBa_I14015/annotation1017909/range1017909/1
type: Range
persistent identity: range1017909
display id: range1017909
version: 1
direction: direction/forward
top level: las TetO
start: 1
end: 151
orientation: inline

TetR
type: SequenceAnnotation
persistent identity: annotation1017910
display id: annotation1017910
version: 1
top level: las TetO
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I14015/annotation1017910/range1017910/1

https://synbiohub.org/public/igem/BBa_I14015/annotation1017910/range1017910/1
type: Range
persistent identity: range1017910
display id: range1017910
version: 1
direction: direction/forward
top level: las TetO
start: 152
end: 170
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1026116
display id: annotation1026116
version: 1
top level: las TetO
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I14015/annotation1026116/range1026116/1

https://synbiohub.org/public/igem/BBa_I14015/annotation1026116/range1026116/1
type: Range
persistent identity: range1026116
display id: range1026116
version: 1
direction: direction/forward
top level: las TetO
start: 140
end: 145
orientation: inline

BBa_K427003
type: ComponentDefinition
persistent identity: BBa K427003
display id: BBa_K427003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Pm promoter of Mu bacteriophage
creator: Jan Marte Contreras Ortiz
created: 2010-10-21T11:00:00Z
modified: 2015-05-08T01:12:29Z
discontinued: false
dominant: false
group u list: _544_
m user id: 0
owner id: 6602
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Pm is the middle promoter of the Mu bacteriophage. It is in charge of the transcription of the activator protein C. Pm is activated by a product of the early transcription, protein Mor. It can be used to build a sensitivity tuner alongside this protein, this construct can be used to increase the amount of POPS in a certain genetic construction.
mutable notes: No special considerations
mutable provenance: Synthetized from the reported sequence of Pm promoter from Mu bacteriophage.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: Pm
sequence: https://synbiohub.org/public/igem/BBa_K427003_sequence/1

Pm
type: SequenceAnnotation
persistent identity: annotation2092367
display id: annotation2092367
version: 1
top level: BBa_K427003
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K427003/annotation2092367/range2092367/1

https://synbiohub.org/public/igem/BBa_K427003/annotation2092367/range2092367/1
type: Range
persistent identity: range2092367
display id: range2092367
version: 1
direction: direction/forward
top level: BBa_K427003
start: 1
end: 71
orientation: inline

BBa_J61054
type: ComponentDefinition
persistent identity: BBa J61054
display id: BBa_J61054
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: [HIP-1] Promoter
creator: John Anderson
created: 2007-02-20T12:00:00Z
modified: 2015-08-31T02:03:00Z
discontinued: false
dominant: false
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: non
mutable notes: non
mutable provenance: non
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J61054_sequence/1

RBS-spoVG
type: ComponentDefinition
persistent identity: BBa K143021
display id: BBa_K143021
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: SpoVG ribosome binding site (RBS) for B. subtilis
creator: James Chappell
created: 2008-09-16T11:00:00Z
modified: 2015-05-08T01:10:23Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _199_
m user id: 0
owner id: 2090
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Description: SpoVG is an endogenous ribosome binding site from B.subtilis. The sequence of the spoVG ribosome binding site is AAAGGUGGUGA which is complementary to the sequence UUUCCUCCACU from the 3' region of the 16s rRNA from B.subtilis. Previous research showed that the predicted binding energy of the 16s rRNA to the RBS is -19kcal 1
mutable notes: In order to ensure that the RBS is functional the actual ribosome binding site was maintained and the distance between the RBS and the start codon maintained. In order to conform to the biobrick standard the sequence flanking the RBS had to be changed but the distance between the promoter and RBS, and start codon and RBS was maintained.
mutable provenance: The sequence was taken from a previous research paper [1] and was constructed by Geneart.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: rbs
sequence: https://synbiohub.org/public/igem/BBa_K143021_sequence/1

rbs
type: SequenceAnnotation
persistent identity: annotation1975997
display id: annotation1975997
version: 1
top level: RBS-spoVG
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K143021/annotation1975997/range1975997/1

https://synbiohub.org/public/igem/BBa_K143021/annotation1975997/range1975997/1
type: Range
persistent identity: range1975997
display id: range1975997
version: 1
direction: direction/forward
top level: RBS-spoVG
start: 1
end: 12
orientation: inline

Prm+
type: ComponentDefinition
persistent identity: BBa I12001
display id: BBa_I12001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter (PRM+)
creator: mcnamara
created: 2004-07-11T11:00:00Z
modified: 2015-08-31T04:07:31Z
discontinued: false
dominant: false
experience: experience/None
group u list: _3_
m user id: 0
owner id: 147
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I12001_sequence/1

U097NUL+D0
type: ComponentDefinition
persistent identity: BBa I732409
display id: BBa_I732409
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Family Member (U097NUL+D026O22)
creator: Zhan Jian
created: 2007-09-19T11:00:00Z
modified: 2015-08-31T04:07:58Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1572
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: A Double Reporter (LacZ-alpha and GFP) loaded on pSB3C5. For more information please refer to BBa_I732403.
mutable notes: Might be later edited.
mutable provenance: Might be later edited.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: polya
sequence annotation: stem_loop
sequence annotation: BBa_B0034
sequence annotation: conserved
sequence annotation: lacZ
sequence annotation: BamHI
sequence annotation: BBa_E0040
sequence annotation: T7 TE
sequence annotation: GFP protein
sequence annotation: BBa_B0012
sequence annotation: STOP
sequence annotation: BBa_I732006
sequence annotation: START
sequence annotation: BBa_B0010
sequence annotation: BglII
sequence annotation: stop
sequence annotation: BBa_I732007
sequence: https://synbiohub.org/public/igem/BBa_I732409_sequence/1

polya
type: SequenceAnnotation
persistent identity: annotation1944534
display id: annotation1944534
version: 1
top level: U097NUL+D0
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_I732409/annotation1944534/range1944534/1

https://synbiohub.org/public/igem/BBa_I732409/annotation1944534/range1944534/1
type: Range
persistent identity: range1944534
display id: range1944534
version: 1
direction: direction/forward
top level: U097NUL+D0
start: 1149
end: 1162
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation1944531
display id: annotation1944531
version: 1
top level: U097NUL+D0
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_I732409/annotation1944531/range1944531/1

https://synbiohub.org/public/igem/BBa_I732409/annotation1944531/range1944531/1
type: Range
persistent identity: range1944531
display id: range1944531
version: 1
direction: direction/forward
top level: U097NUL+D0
start: 1045
end: 1088
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1944527
display id: annotation1944527
version: 1
top level: U097NUL+D0
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_I732409/annotation1944527/range1944527/1

https://synbiohub.org/public/igem/BBa_I732409/annotation1944527/range1944527/1
type: Range
persistent identity: range1944527
display id: range1944527
version: 1
direction: direction/reverse
top level: U097NUL+D0
start: 288
end: 299
orientation: inline

conserved
type: SequenceAnnotation
persistent identity: annotation1944520
display id: annotation1944520
version: 1
top level: U097NUL+D0
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_I732409/annotation1944520/range1944520/1

https://synbiohub.org/public/igem/BBa_I732409/annotation1944520/range1944520/1
type: Range
persistent identity: range1944520
display id: range1944520
version: 1
direction: direction/forward
top level: U097NUL+D0
start: 32
end: 35
orientation: inline

lacZ
type: SequenceAnnotation
persistent identity: annotation1944523
display id: annotation1944523
version: 1
top level: U097NUL+D0
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_I732409/annotation1944523/range1944523/1

https://synbiohub.org/public/igem/BBa_I732409/annotation1944523/range1944523/1
type: Range
persistent identity: range1944523
display id: range1944523
version: 1
direction: direction/forward
top level: U097NUL+D0
start: 46
end: 279
orientation: inline

BamHI
type: SequenceAnnotation
persistent identity: annotation1944518
display id: annotation1944518
version: 1
top level: U097NUL+D0
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I732409/annotation1944518/range1944518/1

https://synbiohub.org/public/igem/BBa_I732409/annotation1944518/range1944518/1
type: Range
persistent identity: range1944518
display id: range1944518
version: 1
direction: direction/forward
top level: U097NUL+D0
start: 12
end: 17
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation1944529
display id: annotation1944529
version: 1
top level: U097NUL+D0
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_I732409/annotation1944529/range1944529/1

https://synbiohub.org/public/igem/BBa_I732409/annotation1944529/range1944529/1
type: Range
persistent identity: range1944529
display id: range1944529
version: 1
direction: direction/reverse
top level: U097NUL+D0
start: 306
end: 1025
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1944533
display id: annotation1944533
version: 1
top level: U097NUL+D0
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_I732409/annotation1944533/range1944533/1

https://synbiohub.org/public/igem/BBa_I732409/annotation1944533/range1944533/1
type: Range
persistent identity: range1944533
display id: range1944533
version: 1
direction: direction/forward
top level: U097NUL+D0
start: 1129
end: 1148
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1944521
display id: annotation1944521
version: 1
top level: U097NUL+D0
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_I732409/annotation1944521/range1944521/1

https://synbiohub.org/public/igem/BBa_I732409/annotation1944521/range1944521/1
type: Range
persistent identity: range1944521
display id: range1944521
version: 1
direction: direction/reverse
top level: U097NUL+D0
start: 28
end: 39
orientation: inline

GFP protein
type: SequenceAnnotation
persistent identity: annotation1944528
display id: annotation1944528
version: 1
top level: U097NUL+D0
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_I732409/annotation1944528/range1944528/1

https://synbiohub.org/public/igem/BBa_I732409/annotation1944528/range1944528/1
type: Range
persistent identity: range1944528
display id: range1944528
version: 1
direction: direction/forward
top level: U097NUL+D0
start: 306
end: 1025
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1944532
display id: annotation1944532
version: 1
top level: U097NUL+D0
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_I732409/annotation1944532/range1944532/1

https://synbiohub.org/public/igem/BBa_I732409/annotation1944532/range1944532/1
type: Range
persistent identity: range1944532
display id: range1944532
version: 1
direction: direction/forward
top level: U097NUL+D0
start: 1122
end: 1162
orientation: inline

STOP
type: SequenceAnnotation
persistent identity: annotation1944524
display id: annotation1944524
version: 1
top level: U097NUL+D0
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_I732409/annotation1944524/range1944524/1

https://synbiohub.org/public/igem/BBa_I732409/annotation1944524/range1944524/1
type: Range
persistent identity: range1944524
display id: range1944524
version: 1
direction: direction/forward
top level: U097NUL+D0
start: 274
end: 279
orientation: inline

BBa_I732006
type: SequenceAnnotation
persistent identity: annotation1944525
display id: annotation1944525
version: 1
top level: U097NUL+D0
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_I732409/annotation1944525/range1944525/1

https://synbiohub.org/public/igem/BBa_I732409/annotation1944525/range1944525/1
type: Range
persistent identity: range1944525
display id: range1944525
version: 1
direction: direction/reverse
top level: U097NUL+D0
start: 46
end: 279
orientation: inline

START
type: SequenceAnnotation
persistent identity: annotation1944522
display id: annotation1944522
version: 1
top level: U097NUL+D0
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_I732409/annotation1944522/range1944522/1

https://synbiohub.org/public/igem/BBa_I732409/annotation1944522/range1944522/1
type: Range
persistent identity: range1944522
display id: range1944522
version: 1
direction: direction/forward
top level: U097NUL+D0
start: 46
end: 48
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1944530
display id: annotation1944530
version: 1
top level: U097NUL+D0
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_I732409/annotation1944530/range1944530/1

https://synbiohub.org/public/igem/BBa_I732409/annotation1944530/range1944530/1
type: Range
persistent identity: range1944530
display id: range1944530
version: 1
direction: direction/forward
top level: U097NUL+D0
start: 1034
end: 1113
orientation: inline

BglII
type: SequenceAnnotation
persistent identity: annotation1944517
display id: annotation1944517
version: 1
top level: U097NUL+D0
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I732409/annotation1944517/range1944517/1

https://synbiohub.org/public/igem/BBa_I732409/annotation1944517/range1944517/1
type: Range
persistent identity: range1944517
display id: range1944517
version: 1
direction: direction/forward
top level: U097NUL+D0
start: 3
end: 8
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1944535
display id: annotation1944535
version: 1
top level: U097NUL+D0
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_I732409/annotation1944535/range1944535/1

https://synbiohub.org/public/igem/BBa_I732409/annotation1944535/range1944535/1
type: Range
persistent identity: range1944535
display id: range1944535
version: 1
direction: direction/forward
top level: U097NUL+D0
start: 1155
end: 1155
orientation: inline

BBa_I732007
type: SequenceAnnotation
persistent identity: annotation1944519
display id: annotation1944519
version: 1
top level: U097NUL+D0
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_I732409/annotation1944519/range1944519/1

https://synbiohub.org/public/igem/BBa_I732409/annotation1944519/range1944519/1
type: Range
persistent identity: range1944519
display id: range1944519
version: 1
direction: direction/reverse
top level: U097NUL+D0
start: 1
end: 19
orientation: inline

conserved
type: SequenceAnnotation
persistent identity: annotation1944526
display id: annotation1944526
version: 1
top level: U097NUL+D0
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_I732409/annotation1944526/range1944526/1

https://synbiohub.org/public/igem/BBa_I732409/annotation1944526/range1944526/1
type: Range
persistent identity: range1944526
display id: range1944526
version: 1
direction: direction/forward
top level: U097NUL+D0
start: 292
end: 295
orientation: inline

BBa_K165043
type: ComponentDefinition
persistent identity: BBa K165043
display id: BBa_K165043
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Zif268-HIV binding sites + MET25 constitutive yeast promoter
creator: John Szymanski
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:10:56Z
discontinued: false
dominant: false
group u list: _267_
m user id: 0
owner id: 2512
owning group id: 58
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: -
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
component: BBa_K165011
component: BBa_K165000
sequence annotation: BBa_K165011
sequence annotation: BBa_K165000
sequence: https://synbiohub.org/public/igem/BBa_K165043_sequence/1

BBa_K165011
type: Component
persistent identity: component1996569
display id: component1996569
version: 1
top level: BBa_K165043
access: public
definition: https://synbiohub.org/public/igem/BBa_K165011/1

BBa_K165000
type: Component
persistent identity: component1996570
display id: component1996570
version: 1
top level: BBa_K165043
access: public
definition: BBa_K165000

BBa_K165011
type: SequenceAnnotation
persistent identity: annotation1996569
display id: annotation1996569
version: 1
top level: BBa_K165043
location: https://synbiohub.org/public/igem/BBa_K165043/annotation1996569/range1996569/1
component: BBa_K165011

https://synbiohub.org/public/igem/BBa_K165043/annotation1996569/range1996569/1
type: Range
persistent identity: range1996569
display id: range1996569
version: 1
direction: direction/reverse
top level: BBa_K165043
start: 1
end: 46
orientation: inline

BBa_K165000
type: SequenceAnnotation
persistent identity: annotation1996570
display id: annotation1996570
version: 1
top level: BBa_K165043
location: https://synbiohub.org/public/igem/BBa_K165043/annotation1996570/range1996570/1
component: BBa_K165000

https://synbiohub.org/public/igem/BBa_K165043/annotation1996570/range1996570/1
type: Range
persistent identity: range1996570
display id: range1996570
version: 1
direction: direction/reverse
top level: BBa_K165043
start: 55
end: 441
orientation: inline

BBa_I712074
type: ComponentDefinition
persistent identity: BBa I712074
display id: BBa_I712074
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: T7 promoter (strong promoter from T7 bacteriophage)
creator: Rok Gaber
created: 2007-10-21T11:00:00Z
modified: 2015-08-31T04:07:46Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _130_
m user id: 0
owner id: 1856
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: T7 promoter is very specific promoter which is transcribed only by specific T7 RNA polymerase. Usually this promoter is used in expression systems where T7 promoter is cotransfected with T7 RNA polymerase. That ensures strong transcription of desired genes.
mutable provenance: T7 bacteriophage
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I712074_sequence/1

BBa_K266005
type: ComponentDefinition
persistent identity: BBa K266005
display id: BBa_K266005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PAI+LasR -> LasI & AI+LuxR --| LasI
creator: UNAM FCIENCIAS-CINVESTAV IRA
created: 2009-10-12T11:00:00Z
modified: 2015-05-08T01:11:43Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _363_
m user id: 0
owner id: 1955
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: BBa_K091146 promoter (PAI+LasR inducible & AI+LuxR repressible) controls the expresion of LasI enzyme, no terminator
mutable notes: None
mutable provenance: The biobricks BBa_K091146 and BBa_S03154 where used to build this biobrick
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Signalling
role: SO:0000804
component: BBa_K091146
component: BBa_S03154
sequence annotation: BBa_K091146
sequence annotation: BBa_S03154
sequence: https://synbiohub.org/public/igem/BBa_K266005_sequence/1

BBa_K091146
type: Component
persistent identity: component2246677
display id: component2246677
version: 1
top level: BBa_K266005
access: public
definition: BBa_K091146

BBa_S03154
type: Component
persistent identity: component2246684
display id: component2246684
version: 1
top level: BBa_K266005
access: public
definition: https://synbiohub.org/public/igem/BBa_S03154/1

BBa_K091146
type: SequenceAnnotation
persistent identity: annotation2246677
display id: annotation2246677
version: 1
top level: BBa_K266005
location: https://synbiohub.org/public/igem/BBa_K266005/annotation2246677/range2246677/1
component: BBa_K091146

https://synbiohub.org/public/igem/BBa_K266005/annotation2246677/range2246677/1
type: Range
persistent identity: range2246677
display id: range2246677
version: 1
direction: direction/reverse
top level: BBa_K266005
start: 1
end: 126
orientation: inline

BBa_S03154
type: SequenceAnnotation
persistent identity: annotation2246684
display id: annotation2246684
version: 1
top level: BBa_K266005
location: https://synbiohub.org/public/igem/BBa_K266005/annotation2246684/range2246684/1
component: BBa_S03154

https://synbiohub.org/public/igem/BBa_K266005/annotation2246684/range2246684/1
type: Range
persistent identity: range2246684
display id: range2246684
version: 1
direction: direction/reverse
top level: BBa_K266005
start: 135
end: 819
orientation: inline

BBa_K284000
type: ComponentDefinition
persistent identity: BBa K284000
display id: BBa_K284000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Lactate Permease from Kluyveromyces lactis
creator: Ra??ssa Estrela Curado, Ta??s Sineiro Herig and Gleidson Silva Teixeira
created: 2009-08-15T11:00:00Z
modified: 2015-05-08T01:11:48Z
discontinued: false
dominant: false
experience: experience/None
group u list: _386_
m user id: 0
owner id: 4968
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: JEN1 from Kluyveromyces lactis is a homologue gene to JEN1 of Saccharomyces cerevisiae. It ecodes a monocarboxylate acids permease shared by lactic and pyruvic acids. Queiros O, Pereira L, Paiva S, Moradas-Ferreira P and Casal M. 2007. Functional analysis of Kluyveromyces lactis carboxylic acids permeases: heterologous expression of KlJEN1 and KlJEN2 genes. Curr Genet 51, 161-169.
mutable notes: Working yet.
mutable provenance: This part was amplified by PCR from K. lactis genomic DNA.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: primer_binding
sequence annotation: Start
sequence annotation: Stop
sequence annotation: Lactate Permease
sequence: https://synbiohub.org/public/igem/BBa_K284000_sequence/1

primer_binding
type: SequenceAnnotation
persistent identity: annotation2027263
display id: annotation2027263
version: 1
top level: BBa_K284000
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K284000/annotation2027263/range2027263/1

https://synbiohub.org/public/igem/BBa_K284000/annotation2027263/range2027263/1
type: Range
persistent identity: range2027263
display id: range2027263
version: 1
direction: direction/reverse
top level: BBa_K284000
start: 1855
end: 1873
orientation: inline

Start
type: SequenceAnnotation
persistent identity: annotation2027261
display id: annotation2027261
version: 1
top level: BBa_K284000
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K284000/annotation2027261/range2027261/1

https://synbiohub.org/public/igem/BBa_K284000/annotation2027261/range2027261/1
type: Range
persistent identity: range2027261
display id: range2027261
version: 1
direction: direction/forward
top level: BBa_K284000
start: 7
end: 9
orientation: inline

primer_binding
type: SequenceAnnotation
persistent identity: annotation2027259
display id: annotation2027259
version: 1
top level: BBa_K284000
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K284000/annotation2027259/range2027259/1

https://synbiohub.org/public/igem/BBa_K284000/annotation2027259/range2027259/1
type: Range
persistent identity: range2027259
display id: range2027259
version: 1
direction: direction/forward
top level: BBa_K284000
start: 1
end: 21
orientation: inline

Stop
type: SequenceAnnotation
persistent identity: annotation2027262
display id: annotation2027262
version: 1
top level: BBa_K284000
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K284000/annotation2027262/range2027262/1

https://synbiohub.org/public/igem/BBa_K284000/annotation2027262/range2027262/1
type: Range
persistent identity: range2027262
display id: range2027262
version: 1
direction: direction/forward
top level: BBa_K284000
start: 1807
end: 1809
orientation: inline

Lactate Permease
type: SequenceAnnotation
persistent identity: annotation2027260
display id: annotation2027260
version: 1
top level: BBa_K284000
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K284000/annotation2027260/range2027260/1

https://synbiohub.org/public/igem/BBa_K284000/annotation2027260/range2027260/1
type: Range
persistent identity: range2027260
display id: range2027260
version: 1
direction: direction/forward
top level: BBa_K284000
start: 7
end: 1809
orientation: inline

BBa_J61106
type: ComponentDefinition
persistent identity: BBa J61106
display id: BBa_J61106
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Ribosome Binding Site Family Member
creator: John Anderson
created: 2007-04-23T11:00:00Z
modified: 2015-08-31T02:03:00Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: false
mutable description: {{JCA_Arkin_RBSFamily}}
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J61106_sequence/1

BBa_K137031
type: ComponentDefinition
persistent identity: BBa K137031
display id: BBa_K137031
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: constitutive promoter with (C)10 between -10 and -35 elements
creator: Allen Lin
created: 2008-07-20T11:00:00Z
modified: 2015-05-08T01:10:09Z
discontinued: false
dominant: false
experience: experience/Issues
group u list: _187_
m user id: 0
owner id: 3112
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: constitutive promoter with (C)10 between -10 and -35 elements
mutable notes: With 10 C repeats between the -10 and -35 elements, the distance between the two elements is optimal for the sigma factor to bind to the promoter. Thus, the promoter should be in the 'on' state.
mutable provenance: This part was PCR amplified from two synthesized primers that bound to each other.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -10
sequence annotation: C repeat
sequence: https://synbiohub.org/public/igem/BBa_K137031_sequence/1

-10
type: SequenceAnnotation
persistent identity: annotation1967768
display id: annotation1967768
version: 1
top level: BBa_K137031
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K137031/annotation1967768/range1967768/1

https://synbiohub.org/public/igem/BBa_K137031/annotation1967768/range1967768/1
type: Range
persistent identity: range1967768
display id: range1967768
version: 1
direction: direction/forward
top level: BBa_K137031
start: 50
end: 55
orientation: inline

C repeat
type: SequenceAnnotation
persistent identity: annotation1967769
display id: annotation1967769
version: 1
top level: BBa_K137031
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K137031/annotation1967769/range1967769/1

https://synbiohub.org/public/igem/BBa_K137031/annotation1967769/range1967769/1
type: Range
persistent identity: range1967769
display id: range1967769
version: 1
direction: direction/forward
top level: BBa_K137031
start: 27
end: 36
orientation: inline

BBa_K883404
type: ComponentDefinition
persistent identity: BBa K883404
display id: BBa_K883404
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PLRV Coat Protein gene - Isolated from Marfona potato
creator: Marnix Vlot
created: 2012-09-06T11:00:00Z
modified: 2015-05-08T01:13:40Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1146_
m user id: 0
owner id: 13437
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part encodes the major coat protein of the Potato Leafroll Virus (PLRV). It was isolated from a PLRV infected Marfora potato leaf by iGEM team Wageningen UR 2012. This was done by cDNA synthesis followed by PCR. The PLRV major Coat Protein should be capable of forming a Virus-Like Particle, but this was not (yet) confirmed for this part.
mutable notes: Inapplicable
mutable provenance: PLRV-infected Marfora potato leaf (RNA).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: PLRV Coat Protein
sequence: https://synbiohub.org/public/igem/BBa_K883404_sequence/1

PLRV Coat Protein
type: SequenceAnnotation
persistent identity: annotation2182003
display id: annotation2182003
version: 1
top level: BBa_K883404
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K883404/annotation2182003/range2182003/1

https://synbiohub.org/public/igem/BBa_K883404/annotation2182003/range2182003/1
type: Range
persistent identity: range2182003
display id: range2182003
version: 1
direction: direction/forward
top level: BBa_K883404
start: 1
end: 627
orientation: inline

pLac(B4)
type: ComponentDefinition
persistent identity: BBa K137125
display id: BBa_K137125
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LacI-repressed promoter B4
creator: Josh Michener
created: 2008-10-07T11:00:00Z
modified: 2015-05-08T01:10:11Z
discontinued: false
dominant: false
experience: experience/None
group u list: _187_
m user id: 0
owner id: 253
owning group id: 6
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: LacI-repressible promoter B4 developed by Sidney Cox (http://www.nature.com/msb/journal/v3/n1/full/msb4100187.html). Showed a regulatory range (ON/OFF) of 2.5x10^4.
mutable provenance: Annealed from oligos.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K137125_sequence/1

BBa_K1824895
type: ComponentDefinition
persistent identity: BBa K1824895
display id: BBa_K1824895
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Ptet + RBS
creator: Zixu Wang
created: 2015-09-10T11:00:00Z
modified: 2015-09-11T09:46:05Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2250_
m user id: 26000
owner id: 26000
owning group id: 9
status: status/Planning
bookmark: false
mutable description: A inducible promoter plus a strong RBS B0034, repressed by TetR, induced by tetracycline and aTc.
mutable notes: This part should be used along with TetR.
mutable provenance: B0034 is based on Elowitz repressilator.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1824889
component: BBa_B0034
component: BBa_R0040
component: BBa_K1824890
sequence annotation: BBa_R0040
sequence annotation: BBa_K1824890
sequence annotation: BBa_B0034
sequence annotation: BBa_K1824889
sequence: https://synbiohub.org/public/igem/BBa_K1824895_sequence/1

BBa_K1824889
type: Component
persistent identity: component2452335
display id: component2452335
version: 1
top level: BBa_K1824895
access: public
definition: https://synbiohub.org/public/igem/BBa_K1824889/1

BBa_B0034
type: Component
persistent identity: component2452337
display id: component2452337
version: 1
top level: BBa_K1824895
access: public
definition: BBa_B0034

BBa_R0040
type: Component
persistent identity: component2452330
display id: component2452330
version: 1
top level: BBa_K1824895
access: public
definition: p(tetR)

BBa_K1824890
type: Component
persistent identity: component2452338
display id: component2452338
version: 1
top level: BBa_K1824895
access: public
definition: https://synbiohub.org/public/igem/BBa_K1824890/1

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation2452330
display id: annotation2452330
version: 1
top level: BBa_K1824895
location: https://synbiohub.org/public/igem/BBa_K1824895/annotation2452330/range2452330/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_K1824895/annotation2452330/range2452330/1
type: Range
persistent identity: range2452330
display id: range2452330
version: 1
direction: direction/forward
top level: BBa_K1824895
start: 1
end: 54
orientation: inline

BBa_K1824890
type: SequenceAnnotation
persistent identity: annotation2452338
display id: annotation2452338
version: 1
top level: BBa_K1824895
location: https://synbiohub.org/public/igem/BBa_K1824895/annotation2452338/range2452338/1
component: BBa_K1824890

https://synbiohub.org/public/igem/BBa_K1824895/annotation2452338/range2452338/1
type: Range
persistent identity: range2452338
display id: range2452338
version: 1
direction: direction/reverse
top level: BBa_K1824895
start: 75
end: 80
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2452337
display id: annotation2452337
version: 1
top level: BBa_K1824895
location: https://synbiohub.org/public/igem/BBa_K1824895/annotation2452337/range2452337/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1824895/annotation2452337/range2452337/1
type: Range
persistent identity: range2452337
display id: range2452337
version: 1
direction: direction/reverse
top level: BBa_K1824895
start: 63
end: 74
orientation: inline

BBa_K1824889
type: SequenceAnnotation
persistent identity: annotation2452335
display id: annotation2452335
version: 1
top level: BBa_K1824895
location: https://synbiohub.org/public/igem/BBa_K1824895/annotation2452335/range2452335/1
component: BBa_K1824889

https://synbiohub.org/public/igem/BBa_K1824895/annotation2452335/range2452335/1
type: Range
persistent identity: range2452335
display id: range2452335
version: 1
direction: direction/reverse
top level: BBa_K1824895
start: 55
end: 62
orientation: inline

BGH pA
type: ComponentDefinition
persistent identity: BBa K678019
display id: BBa_K678019
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: BGH poly A, mammalian terminator
creator: DTU-Denmark-2
created: 2011-09-03T11:00:00Z
modified: 2015-05-08T01:13:04Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _882_
m user id: 0
owner id: 8341
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: BGH poly A, terminator for use in mammalian cells
mutable notes: Compatible with the Plug'n'Play assembly standard
mutable provenance: From plasmid pcDNA3.1 from Invitrogen
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BGH
sequence annotation: Linker 4
sequence: https://synbiohub.org/public/igem/BBa_K678019_sequence/1

BGH
type: SequenceAnnotation
persistent identity: annotation2127353
display id: annotation2127353
version: 1
top level: BGH pA
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_K678019/annotation2127353/range2127353/1

https://synbiohub.org/public/igem/BBa_K678019/annotation2127353/range2127353/1
type: Range
persistent identity: range2127353
display id: range2127353
version: 1
direction: direction/forward
top level: BGH pA
start: 9
end: 233
orientation: inline

Linker 4
type: SequenceAnnotation
persistent identity: annotation2127352
display id: annotation2127352
version: 1
top level: BGH pA
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K678019/annotation2127352/range2127352/1

https://synbiohub.org/public/igem/BBa_K678019/annotation2127352/range2127352/1
type: Range
persistent identity: range2127352
display id: range2127352
version: 1
direction: direction/forward
top level: BGH pA
start: 1
end: 8
orientation: inline

BBa_J64011
type: ComponentDefinition
persistent identity: BBa J64011
display id: BBa_J64011
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: invB RBS
creator: Dan Widmaier
created: 2008-01-15T12:00:00Z
modified: 2015-08-31T01:56:26Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _98_
m user id: 0
owner id: 2407
owning group id: 98
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: The natural RBS that drives invB
mutable notes: The expression level of this RBS has not been quantified.
mutable provenance: Salmonella LT2 Genomic DNA
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: invB RBS
sequence: https://synbiohub.org/public/igem/BBa_J64011_sequence/1

invB RBS
type: SequenceAnnotation
persistent identity: annotation1959604
display id: annotation1959604
version: 1
top level: BBa_J64011
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_J64011/annotation1959604/range1959604/1

https://synbiohub.org/public/igem/BBa_J64011/annotation1959604/range1959604/1
type: Range
persistent identity: range1959604
display id: range1959604
version: 1
direction: direction/forward
top level: BBa_J64011
start: 1
end: 19
orientation: inline

BBa_K389050
type: ComponentDefinition
persistent identity: BBa K389050
display id: BBa_K389050
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Tightly controlled lac operon
creator: Jonas Aretz
created: 2010-09-27T11:00:00Z
modified: 2015-05-08T01:12:19Z
discontinued: false
dominant: false
experience: experience/Fails
group u list: _517_
m user id: 0
owner id: 6670
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This BioBrick is a tightly controlled ''lac'' operon. It contains a lacIq and a ''lac'' operator, so it is inducible with IPTG but has a low basal expression. It is also possible to use this BioBrick in strains that do not carry a lacIq like ''E. coli'' TOP10.
mutable notes: we needed a tightly controlled inducible promoter
mutable provenance: partsregistry
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
component: BBa_B0034
component: BBa_I14032
component: BBa_C0012
component: BBa_B0017
component: BBa_R0010
sequence annotation: BBa_B0034
sequence annotation: BBa_C0012
sequence annotation: BBa_B0017
sequence annotation: BBa_I14032
sequence annotation: BBa_R0010
sequence: https://synbiohub.org/public/igem/BBa_K389050_sequence/1

BBa_B0034
type: Component
persistent identity: component2263602
display id: component2263602
version: 1
top level: BBa_K389050
access: public
definition: BBa_B0034

BBa_I14032
type: Component
persistent identity: component2263600
display id: component2263600
version: 1
top level: BBa_K389050
access: public
definition: P(Lac) IQ

BBa_C0012
type: Component
persistent identity: component2263604
display id: component2263604
version: 1
top level: BBa_K389050
access: public
definition: https://synbiohub.org/public/igem/BBa_C0012/1

BBa_B0017
type: Component
persistent identity: component2263611
display id: component2263611
version: 1
top level: BBa_K389050
access: public
definition: BBa_B0017

BBa_R0010
type: Component
persistent identity: component2263612
display id: component2263612
version: 1
top level: BBa_K389050
access: public
definition: LacI

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2263602
display id: annotation2263602
version: 1
top level: BBa_K389050
location: https://synbiohub.org/public/igem/BBa_K389050/annotation2263602/range2263602/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K389050/annotation2263602/range2263602/1
type: Range
persistent identity: range2263602
display id: range2263602
version: 1
direction: direction/reverse
top level: BBa_K389050
start: 46
end: 57
orientation: inline

BBa_C0012
type: SequenceAnnotation
persistent identity: annotation2263604
display id: annotation2263604
version: 1
top level: BBa_K389050
location: https://synbiohub.org/public/igem/BBa_K389050/annotation2263604/range2263604/1
component: BBa_C0012

https://synbiohub.org/public/igem/BBa_K389050/annotation2263604/range2263604/1
type: Range
persistent identity: range2263604
display id: range2263604
version: 1
direction: direction/forward
top level: BBa_K389050
start: 64
end: 1191
orientation: inline

BBa_B0017
type: SequenceAnnotation
persistent identity: annotation2263611
display id: annotation2263611
version: 1
top level: BBa_K389050
location: https://synbiohub.org/public/igem/BBa_K389050/annotation2263611/range2263611/1
component: BBa_B0017

https://synbiohub.org/public/igem/BBa_K389050/annotation2263611/range2263611/1
type: Range
persistent identity: range2263611
display id: range2263611
version: 1
direction: direction/reverse
top level: BBa_K389050
start: 1225
end: 1392
orientation: inline

BBa_I14032
type: SequenceAnnotation
persistent identity: annotation2263600
display id: annotation2263600
version: 1
top level: BBa_K389050
location: https://synbiohub.org/public/igem/BBa_K389050/annotation2263600/range2263600/1
component: BBa_I14032

https://synbiohub.org/public/igem/BBa_K389050/annotation2263600/range2263600/1
type: Range
persistent identity: range2263600
display id: range2263600
version: 1
direction: direction/reverse
top level: BBa_K389050
start: 1
end: 37
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation2263612
display id: annotation2263612
version: 1
top level: BBa_K389050
location: https://synbiohub.org/public/igem/BBa_K389050/annotation2263612/range2263612/1
component: BBa_R0010

https://synbiohub.org/public/igem/BBa_K389050/annotation2263612/range2263612/1
type: Range
persistent identity: range2263612
display id: range2263612
version: 1
direction: direction/forward
top level: BBa_K389050
start: 1401
end: 1600
orientation: inline

BBa_J61131
type: ComponentDefinition
persistent identity: BBa J61131
display id: BBa_J61131
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Ribosome Binding Site Family Member
creator: John Anderson
created: 2007-04-24T11:00:00Z
modified: 2015-08-31T01:59:49Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: false
mutable description: {{JCA_Arkin_RBSFamily}}
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J61131_sequence/1

BBa_J23117
type: ComponentDefinition
persistent identity: BBa J23117
display id: BBa_J23117
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: constitutive promoter family member
creator: John Anderson
created: 2006-08-16T11:00:00Z
modified: 2015-08-31T04:08:40Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: Later
mutable notes: N/A
mutable provenance: Later
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J23117_sequence/1

pLacIQ1
type: ComponentDefinition
persistent identity: BBa K091112
display id: BBa_K091112
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pLacIQ1 promoter
creator: Pallavi Penumetcha
created: 2008-06-19T11:00:00Z
modified: 2015-05-08T01:08:37Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _191_
m user id: 0
owner id: 3080
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: this is a modified version of the LacIQ promoter that should bind LacIQ tighter than the original.
mutable notes: no special consideration
mutable provenance: This is a modified version of the LacIQ promoter.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -10
sequence annotation: -35
sequence: https://synbiohub.org/public/igem/BBa_K091112_sequence/1

-10
type: SequenceAnnotation
persistent identity: annotation1964078
display id: annotation1964078
version: 1
top level: pLacIQ1
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K091112/annotation1964078/range1964078/1

https://synbiohub.org/public/igem/BBa_K091112/annotation1964078/range1964078/1
type: Range
persistent identity: range1964078
display id: range1964078
version: 1
direction: direction/forward
top level: pLacIQ1
start: 37
end: 41
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1964077
display id: annotation1964077
version: 1
top level: pLacIQ1
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K091112/annotation1964077/range1964077/1

https://synbiohub.org/public/igem/BBa_K091112/annotation1964077/range1964077/1
type: Range
persistent identity: range1964077
display id: range1964077
version: 1
direction: direction/forward
top level: pLacIQ1
start: 12
end: 17
orientation: inline

BBa_K124000
type: ComponentDefinition
persistent identity: BBa K124000
display id: BBa_K124000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pCYC Yeast Promoter
creator: John Szymanski
created: 2008-06-30T11:00:00Z
modified: 2015-05-08T01:09:44Z
discontinued: false
dominant: false
group u list: _221_
m user id: 0
owner id: 2512
owning group id: 58
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: The promoter from the yeast gene CYC1. Activity characterized here: http://dx.doi.org/10.1016/0378-1119(95)00037-7.
mutable notes: none
mutable provenance: From Mumberg, Muller, and Funk p413 pCYC. See ref. above
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K124000_sequence/1

BBa_J61115
type: ComponentDefinition
persistent identity: BBa J61115
display id: BBa_J61115
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Ribosome Binding Site Family Member
creator: John Anderson
created: 2007-04-23T11:00:00Z
modified: 2015-08-31T02:03:00Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: false
mutable description: {{JCA_Arkin_RBSFamily}}
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J61115_sequence/1

cI lam
type: ComponentDefinition
persistent identity: BBa R1051
display id: BBa_R1051
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter, Standard (lambda cI regulated)
creator: Vinay S Mahajan, Brian Chow, Peter Carr, Voichita Marinescu and Alexander D. Wissner-Gross Drew Endy
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:15Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: [Note: This is the same part as R0051 except that the -10 and -35 sites and spacing have been changed to comply with BBa_S0001]. The cI regulated promoter is based on the pR promtoer from bacteriohage lambda. The promoter has two two DNA binding sites for lambda cI repressor <bb_part>BBa_C0051</bb_part>. cI binding results in repression of transcription. The specific sequence used here is based on the cI repressible promoter used in the Elowitz repressilator (and references therein).
mutable notes: In order to address concerns about the promoter transcribing in the reverse direction, we have removed the -35 and -10 signals responsible for the promoter activity in the reverse direction. (More details needed here! DE, 2/24/03) Modified to comply with BBa_S0001: TTGACA-17N-GATACT Incompatible with host expressing cI repressor.
mutable provenance: A synthetic oscillatory network of transcriptional regulators , Elowitz M.B. , Leibler S., Nature(403),335-38: 2000
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -10
sequence annotation: OR1
sequence annotation: -35
sequence annotation: OR2
sequence annotation: BBa_R1051
sequence: https://synbiohub.org/public/igem/BBa_R1051_sequence/1

-10
type: SequenceAnnotation
persistent identity: annotation2078
display id: annotation2078
version: 1
top level: cI lam
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R1051/annotation2078/range2078/1

https://synbiohub.org/public/igem/BBa_R1051/annotation2078/range2078/1
type: Range
persistent identity: range2078
display id: range2078
version: 1
direction: direction/forward
top level: cI lam
start: 38
end: 43
orientation: inline

OR1
type: SequenceAnnotation
persistent identity: annotation2076
display id: annotation2076
version: 1
top level: cI lam
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R1051/annotation2076/range2076/1

https://synbiohub.org/public/igem/BBa_R1051/annotation2076/range2076/1
type: Range
persistent identity: range2076
display id: range2076
version: 1
direction: direction/forward
top level: cI lam
start: 25
end: 41
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2075
display id: annotation2075
version: 1
top level: cI lam
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R1051/annotation2075/range2075/1

https://synbiohub.org/public/igem/BBa_R1051/annotation2075/range2075/1
type: Range
persistent identity: range2075
display id: range2075
version: 1
direction: direction/forward
top level: cI lam
start: 15
end: 20
orientation: inline

OR2
type: SequenceAnnotation
persistent identity: annotation2077
display id: annotation2077
version: 1
top level: cI lam
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R1051/annotation2077/range2077/1

https://synbiohub.org/public/igem/BBa_R1051/annotation2077/range2077/1
type: Range
persistent identity: range2077
display id: range2077
version: 1
direction: direction/forward
top level: cI lam
start: 1
end: 17
orientation: inline

BBa_R1051
type: SequenceAnnotation
persistent identity: annotation7074
display id: annotation7074
version: 1
top level: cI lam
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_R1051/annotation7074/range7074/1

https://synbiohub.org/public/igem/BBa_R1051/annotation7074/range7074/1
type: Range
persistent identity: range7074
display id: range7074
version: 1
direction: direction/forward
top level: cI lam
start: 1
end: 49
orientation: inline

U073O11+D0
type: ComponentDefinition
persistent identity: BBa I732403
display id: BBa_I732403
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Family Member (U073O11+D062NUL)
creator: Zhan Jian
created: 2007-09-20T11:00:00Z
modified: 2015-08-31T04:07:58Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1572
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Might be later edited.
mutable notes: Might be later edited.
mutable provenance: Might be later edited.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: O1
sequence annotation: +1
sequence annotation: consensus elements
sequence: https://synbiohub.org/public/igem/BBa_I732403_sequence/1

O1
type: SequenceAnnotation
persistent identity: annotation1944543
display id: annotation1944543
version: 1
top level: U073O11+D0
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_I732403/annotation1944543/range1944543/1

https://synbiohub.org/public/igem/BBa_I732403/annotation1944543/range1944543/1
type: Range
persistent identity: range1944543
display id: range1944543
version: 1
direction: direction/forward
top level: U073O11+D0
start: 1
end: 20
orientation: inline

+1
type: SequenceAnnotation
persistent identity: annotation1944544
display id: annotation1944544
version: 1
top level: U073O11+D0
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_I732403/annotation1944544/range1944544/1

https://synbiohub.org/public/igem/BBa_I732403/annotation1944544/range1944544/1
type: Range
persistent identity: range1944544
display id: range1944544
version: 1
direction: direction/forward
top level: U073O11+D0
start: 93
end: 93
orientation: inline

consensus elements
type: SequenceAnnotation
persistent identity: annotation1944542
display id: annotation1944542
version: 1
top level: U073O11+D0
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I732403/annotation1944542/range1944542/1

https://synbiohub.org/public/igem/BBa_I732403/annotation1944542/range1944542/1
type: Range
persistent identity: range1944542
display id: range1944542
version: 1
direction: direction/forward
top level: U073O11+D0
start: 57
end: 93
orientation: inline

BBa_R2108
type: ComponentDefinition
persistent identity: BBa R2108
display id: BBa_R2108
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter with operator site for C2003
creator: Reshma Shetty
created: 2006-10-20T11:00:00Z
modified: 2015-05-08T01:14:16Z
discontinued: false
dominant: false
group u list: _41_
m user id: 0
owner id: 126
owning group id: 70
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This promoter is designed to bind to C2003.
mutable notes: Placement of the operator sites was optimized for steric interference between the C2003 and RNA polymerase based on structural models.
mutable provenance: This promoter was rationally designed.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: operator site for C2003
sequence annotation: transcription start site
sequence annotation: -10 site
sequence annotation: -35 site
sequence: https://synbiohub.org/public/igem/BBa_R2108_sequence/1

operator site for C2003
type: SequenceAnnotation
persistent identity: annotation1903909
display id: annotation1903909
version: 1
top level: BBa_R2108
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_R2108/annotation1903909/range1903909/1

https://synbiohub.org/public/igem/BBa_R2108/annotation1903909/range1903909/1
type: Range
persistent identity: range1903909
display id: range1903909
version: 1
direction: direction/forward
top level: BBa_R2108
start: 32
end: 43
orientation: inline

transcription start site
type: SequenceAnnotation
persistent identity: annotation1903911
display id: annotation1903911
version: 1
top level: BBa_R2108
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_R2108/annotation1903911/range1903911/1

https://synbiohub.org/public/igem/BBa_R2108/annotation1903911/range1903911/1
type: Range
persistent identity: range1903911
display id: range1903911
version: 1
direction: direction/forward
top level: BBa_R2108
start: 53
end: 53
orientation: inline

-10 site
type: SequenceAnnotation
persistent identity: annotation1903910
display id: annotation1903910
version: 1
top level: BBa_R2108
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R2108/annotation1903910/range1903910/1

https://synbiohub.org/public/igem/BBa_R2108/annotation1903910/range1903910/1
type: Range
persistent identity: range1903910
display id: range1903910
version: 1
direction: direction/forward
top level: BBa_R2108
start: 40
end: 45
orientation: inline

-35 site
type: SequenceAnnotation
persistent identity: annotation1903908
display id: annotation1903908
version: 1
top level: BBa_R2108
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R2108/annotation1903908/range1903908/1

https://synbiohub.org/public/igem/BBa_R2108/annotation1903908/range1903908/1
type: Range
persistent identity: range1903908
display id: range1903908
version: 1
direction: direction/forward
top level: BBa_R2108
start: 17
end: 22
orientation: inline

BBa_K780003
type: ComponentDefinition
persistent identity: BBa K780003
display id: BBa_K780003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Strong constitutive promoter for Bacillus subtilis
creator: Elżbieta Jankowska
created: 2012-09-25T11:00:00Z
modified: 2015-05-08T01:13:18Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1034_
m user id: 0
owner id: 10113
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Strong constitutive promoter for Bacillus subtilis
mutable notes: -
mutable provenance: 'host-takover module' from B.subtilis Bacteriophage SPO1
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K780003_sequence/1

BBa_K2084004
type: ComponentDefinition
persistent identity: BBa K2084004
display id: BBa_K2084004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: T3 Promoter with RBS site
creator: Maya Lemmon-Kishi
created: 2016-10-13T11:00:00Z
modified: 2016-10-29T09:48:58Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2552_
m user id: 32066
owner id: 32131
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This is the T3 promoter with an RBS site added. Other groups should use this part to regulate their part by adding it in front.
mutable notes: NA
mutable provenance: The T3 promoter comes from the T3 bacteriophage. The RBS site comes from...
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
component: BBa_K2084000
component: BBa_B0034
sequence annotation: BBa_K2084000
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K2084004_sequence/1

BBa_K2084000
type: Component
persistent identity: component2531295
display id: component2531295
version: 1
top level: BBa_K2084004
access: public
definition: BBa_K2084000

BBa_B0034
type: Component
persistent identity: component2531297
display id: component2531297
version: 1
top level: BBa_K2084004
access: public
definition: BBa_B0034

BBa_K2084000
type: SequenceAnnotation
persistent identity: annotation2531295
display id: annotation2531295
version: 1
top level: BBa_K2084004
location: https://synbiohub.org/public/igem/BBa_K2084004/annotation2531295/range2531295/1
component: BBa_K2084000

https://synbiohub.org/public/igem/BBa_K2084004/annotation2531295/range2531295/1
type: Range
persistent identity: range2531295
display id: range2531295
version: 1
direction: direction/reverse
top level: BBa_K2084004
start: 1
end: 23
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2531297
display id: annotation2531297
version: 1
top level: BBa_K2084004
location: https://synbiohub.org/public/igem/BBa_K2084004/annotation2531297/range2531297/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K2084004/annotation2531297/range2531297/1
type: Range
persistent identity: range2531297
display id: range2531297
version: 1
direction: direction/reverse
top level: BBa_K2084004
start: 32
end: 43
orientation: inline

lac Or2-62
type: ComponentDefinition
persistent identity: BBa I12210
display id: BBa_I12210
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: plac Or2-62 (positive)
creator: rwald
created: 2004-08-06T11:00:00Z
modified: 2015-08-31T04:07:31Z
discontinued: false
dominant: false
experience: experience/None
group u list: _3_
m user id: 0
owner id: 120
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable description: This is a modification of the standard plac operon with everything before the -35 site removed, the Or2 site from the lambda promoter inserted at the beginning, and a 17-bp spacer added between the two.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -35
sequence annotation: -10
sequence annotation: spacer
sequence annotation: cI OR2 (lambda)
sequence: https://synbiohub.org/public/igem/BBa_I12210_sequence/1

-35
type: SequenceAnnotation
persistent identity: annotation1031725
display id: annotation1031725
version: 1
top level: lac Or2-62
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I12210/annotation1031725/range1031725/1

https://synbiohub.org/public/igem/BBa_I12210/annotation1031725/range1031725/1
type: Range
persistent identity: range1031725
display id: range1031725
version: 1
direction: direction/forward
top level: lac Or2-62
start: 35
end: 40
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1031726
display id: annotation1031726
version: 1
top level: lac Or2-62
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I12210/annotation1031726/range1031726/1

https://synbiohub.org/public/igem/BBa_I12210/annotation1031726/range1031726/1
type: Range
persistent identity: range1031726
display id: range1031726
version: 1
direction: direction/forward
top level: lac Or2-62
start: 59
end: 65
orientation: inline

spacer
type: SequenceAnnotation
persistent identity: annotation1031727
display id: annotation1031727
version: 1
top level: lac Or2-62
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I12210/annotation1031727/range1031727/1

https://synbiohub.org/public/igem/BBa_I12210/annotation1031727/range1031727/1
type: Range
persistent identity: range1031727
display id: range1031727
version: 1
direction: direction/forward
top level: lac Or2-62
start: 18
end: 34
orientation: inline

cI OR2 (lambda)
type: SequenceAnnotation
persistent identity: annotation1031724
display id: annotation1031724
version: 1
top level: lac Or2-62
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I12210/annotation1031724/range1031724/1

https://synbiohub.org/public/igem/BBa_I12210/annotation1031724/range1031724/1
type: Range
persistent identity: range1031724
display id: range1031724
version: 1
direction: direction/forward
top level: lac Or2-62
start: 1
end: 17
orientation: inline

BBa_M36888
type: ComponentDefinition
persistent identity: BBa M36888
display id: BBa_M36888
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Sequence for sigma 32 transcription factor
creator: Shamik Mascharak, Justo Caballero and Sibel Sayiner
created: 2012-12-05T12:00:00Z
modified: 2015-05-08T01:14:06Z
discontinued: false
dominant: false
experience: experience/Issues
group u list: _848_
m user id: 0
owner id: 15030
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: The rpoH gene codes for sigma 32 (alternatively sigma H, RpoH) transcription factor, a component of RNA polymerase sigma 32 holoenzyme. This 32-kD stress factor belongs to the sigma-70 factor family and is involved in intracellular temperature regulation, initiating bacterial transcription of various chaperone proteins and proteases in response to sudden increases in ambient temperature. The gene is currently classified as essential, due to the fact that rpoH deletion mutants fail to grow at standard incubation temperatures. This part may be employed in heat shock-to-PoPs sensors by coupling expression of the rpoH gene to action of sigma 32-inducible promoters such as the htpG heat-shock promoter (part#: BBa_J45405). Transcriptional activation by rpoH transcripts is possible both within and without the sensor genetic circuit, as sigma factors can diffuse around the nucleus.
mutable notes: Designs incorporating this part into a feedback amplification loop must beware of likely rpoH-deletion mutants. This is due to overproduction of sigma 32 under leaky promoters such as htpG (BBa_J45405). Less deletion mutants in E. coli are noted when plasmids containing rpoH are cloned at higher temperature. Consider two facts before using this part. First, rpoH transcript activity is reduced under excess heat shock protein production. As such, feedback loops using rpoH should employ weaker bicistronic ribosome binding sites so as to optimize heat-shock response. Second, sigma 32 is turned over rapidly, with a half-life under 1 minute.
mutable provenance: The particular part described here is specific to the genome of W3110 strain E. coli.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: STOP
sequence annotation: START
sequence: https://synbiohub.org/public/igem/BBa_M36888_sequence/1

STOP
type: SequenceAnnotation
persistent identity: annotation2213640
display id: annotation2213640
version: 1
top level: BBa_M36888
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_M36888/annotation2213640/range2213640/1

https://synbiohub.org/public/igem/BBa_M36888/annotation2213640/range2213640/1
type: Range
persistent identity: range2213640
display id: range2213640
version: 1
direction: direction/forward
top level: BBa_M36888
start: 853
end: 861
orientation: inline

START
type: SequenceAnnotation
persistent identity: annotation2213636
display id: annotation2213636
version: 1
top level: BBa_M36888
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_M36888/annotation2213636/range2213636/1

https://synbiohub.org/public/igem/BBa_M36888/annotation2213636/range2213636/1
type: Range
persistent identity: range2213636
display id: range2213636
version: 1
direction: direction/forward
top level: BBa_M36888
start: 1
end: 3
orientation: inline

BBa_M13102
type: ComponentDefinition
persistent identity: BBa M13102
display id: BBa_M13102
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: M13K07 gene II promoter
creator: Natalie Kuldell
created: 2006-12-15T12:00:00Z
modified: 2015-05-08T01:13:55Z
discontinued: false
dominant: true
experience: experience/None
group u list: _45_
m user id: 0
owner id: 314
owning group id: 1
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This part is from the bacteriophage M13 genome. It directs transcription of M13 gene II (BBa_M13502, BBa_M13002). Promoter identified in Gene (1980) 11:129-148 based on -10,-35 homology and fragment of genome able to bind RNAP.
mutable notes: none
mutable provenance: New England Biolabs M13K07. Sequenced in 2002 by L. Panganaban.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_M13102_sequence/1

BBa_R0184
type: ComponentDefinition
persistent identity: BBa R0184
display id: BBa_R0184
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: T7 promoter (lacI repressible)
creator: Bartholomew Canton
created: 2007-04-25T11:00:00Z
modified: 2015-05-08T01:14:15Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_
m user id: 0
owner id: 135
owning group id: 84
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: A mutant version of the T7lac promoter designed by Dubendorff and Studier. The promoter is based on the strong ??10 T7 promoter but incorporates an A->C mutation at -16. The LacI operator site is a partially symmetric 25bp sequence.
mutable notes: The -16 mutation was included to weaken the consensus promoter as described by Imburgio and co-workers (see BBa_R0183 for more information)
mutable provenance: This sequence is based on the T7lac promoter described by Dubendorff and Studier (JMB 1991, 219, 45-59). The only modification is the mutation at -16.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: lacI binding site
sequence annotation: Center of binding site symmetry
sequence annotation: BBa_R0183 fragment
sequence: https://synbiohub.org/public/igem/BBa_R0184_sequence/1

lacI binding site
type: SequenceAnnotation
persistent identity: annotation1932679
display id: annotation1932679
version: 1
top level: BBa_R0184
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_R0184/annotation1932679/range1932679/1

https://synbiohub.org/public/igem/BBa_R0184/annotation1932679/range1932679/1
type: Range
persistent identity: range1932679
display id: range1932679
version: 1
direction: direction/forward
top level: BBa_R0184
start: 20
end: 44
orientation: inline

Center of binding site symmetry
type: SequenceAnnotation
persistent identity: annotation1932680
display id: annotation1932680
version: 1
top level: BBa_R0184
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_R0184/annotation1932680/range1932680/1

https://synbiohub.org/public/igem/BBa_R0184/annotation1932680/range1932680/1
type: Range
persistent identity: range1932680
display id: range1932680
version: 1
direction: direction/forward
top level: BBa_R0184
start: 31
end: 31
orientation: inline

BBa_R0183 fragment
type: SequenceAnnotation
persistent identity: annotation1932678
display id: annotation1932678
version: 1
top level: BBa_R0184
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0184/annotation1932678/range1932678/1

https://synbiohub.org/public/igem/BBa_R0184/annotation1932678/range1932678/1
type: Range
persistent identity: range1932678
display id: range1932678
version: 1
direction: direction/forward
top level: BBa_R0184
start: 1
end: 20
orientation: inline

BBa_R0182
type: ComponentDefinition
persistent identity: BBa R0182
display id: BBa_R0182
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: T7 RNAP promoter
creator: Barry Canton
created: 2005-09-25T11:00:00Z
modified: 2015-05-08T01:14:15Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_6_
m user id: 0
owner id: 135
owning group id: 6
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: T7 promoter with an A->T mutation at the -10 position of the consensus sequence
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_R0182_sequence/1

BBa_J64809
type: ComponentDefinition
persistent identity: BBa J64809
display id: BBa_J64809
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RocF RBS
creator: David Stanley
created: 2007-03-19T12:00:00Z
modified: 2015-05-08T01:08:17Z
discontinued: false
dominant: false
experience: experience/None
group u list: _98_
m user id: 0
owner id: 1423
owning group id: 98
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: RBS upstream of RocF
mutable notes: n
mutable provenance: n
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J64809_sequence/1

pJEJAM12
type: ComponentDefinition
persistent identity: BBa K678060
display id: BBa_K678060
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pJEJAM12
creator: DTU-Denmark-2
created: 2011-09-12T11:00:00Z
modified: 2015-05-08T01:13:05Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _882_
m user id: 0
owner id: 8341
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: To be announced
mutable notes: To be announced
mutable provenance: To be announced
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000155
role: partType/Plasmid
component: BBa_K678024
component: BBa_K678027
component: BBa_K678040
component: BBa_K678036
component: BBa_K678046
sequence annotation: BBa_K678027
sequence annotation: BBa_K678024
sequence annotation: BBa_K678036
sequence annotation: BBa_K678040
sequence annotation: BBa_K678046
sequence: https://synbiohub.org/public/igem/BBa_K678060_sequence/1

BBa_K678024
type: Component
persistent identity: component2135896
display id: component2135896
version: 1
top level: pJEJAM12
access: public
definition: PgpdA

BBa_K678027
type: Component
persistent identity: component2135899
display id: component2135899
version: 1
top level: pJEJAM12
access: public
definition: GFP

BBa_K678040
type: Component
persistent identity: component2135905
display id: component2135905
version: 1
top level: pJEJAM12
access: public
definition: pyrG casse

BBa_K678036
type: Component
persistent identity: component2135902
display id: component2135902
version: 1
top level: pJEJAM12
access: public
definition: TtrpC

BBa_K678046
type: Component
persistent identity: component2135908
display id: component2135908
version: 1
top level: pJEJAM12
access: public
definition: pFun

BBa_K678027
type: SequenceAnnotation
persistent identity: annotation2135899
display id: annotation2135899
version: 1
top level: pJEJAM12
location: https://synbiohub.org/public/igem/BBa_K678060/annotation2135899/range2135899/1
component: BBa_K678027

https://synbiohub.org/public/igem/BBa_K678060/annotation2135899/range2135899/1
type: Range
persistent identity: range2135899
display id: range2135899
version: 1
direction: direction/reverse
top level: pJEJAM12
start: 2313
end: 3040
orientation: inline

BBa_K678024
type: SequenceAnnotation
persistent identity: annotation2135896
display id: annotation2135896
version: 1
top level: pJEJAM12
location: https://synbiohub.org/public/igem/BBa_K678060/annotation2135896/range2135896/1
component: BBa_K678024

https://synbiohub.org/public/igem/BBa_K678060/annotation2135896/range2135896/1
type: Range
persistent identity: range2135896
display id: range2135896
version: 1
direction: direction/reverse
top level: pJEJAM12
start: 1
end: 2312
orientation: inline

BBa_K678036
type: SequenceAnnotation
persistent identity: annotation2135902
display id: annotation2135902
version: 1
top level: pJEJAM12
location: https://synbiohub.org/public/igem/BBa_K678060/annotation2135902/range2135902/1
component: BBa_K678036

https://synbiohub.org/public/igem/BBa_K678060/annotation2135902/range2135902/1
type: Range
persistent identity: range2135902
display id: range2135902
version: 1
direction: direction/reverse
top level: pJEJAM12
start: 3041
end: 3799
orientation: inline

BBa_K678040
type: SequenceAnnotation
persistent identity: annotation2135905
display id: annotation2135905
version: 1
top level: pJEJAM12
location: https://synbiohub.org/public/igem/BBa_K678060/annotation2135905/range2135905/1
component: BBa_K678040

https://synbiohub.org/public/igem/BBa_K678060/annotation2135905/range2135905/1
type: Range
persistent identity: range2135905
display id: range2135905
version: 1
direction: direction/reverse
top level: pJEJAM12
start: 3800
end: 5255
orientation: inline

BBa_K678046
type: SequenceAnnotation
persistent identity: annotation2135908
display id: annotation2135908
version: 1
top level: pJEJAM12
location: https://synbiohub.org/public/igem/BBa_K678060/annotation2135908/range2135908/1
component: BBa_K678046

https://synbiohub.org/public/igem/BBa_K678060/annotation2135908/range2135908/1
type: Range
persistent identity: range2135908
display id: range2135908
version: 1
direction: direction/reverse
top level: pJEJAM12
start: 5256
end: 8133
orientation: inline

BBa_K864601
type: ComponentDefinition
persistent identity: BBa K864601
display id: BBa_K864601
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Lambda t1 transcriptional terminator
creator: Erik Lundin
created: 2012-09-24T11:00:00Z
modified: 2015-05-08T01:13:37Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1124_
m user id: 0
owner id: 9827
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: The lambda t1 is a strong transcriptional terminator with 99% efficiency in vivo.
mutable notes: -
mutable provenance: Gene synthesis
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: lambda t1 terminator
sequence: https://synbiohub.org/public/igem/BBa_K864601_sequence/1

lambda t1 terminator
type: SequenceAnnotation
persistent identity: annotation2197328
display id: annotation2197328
version: 1
top level: BBa_K864601
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K864601/annotation2197328/range2197328/1

https://synbiohub.org/public/igem/BBa_K864601/annotation2197328/range2197328/1
type: Range
persistent identity: range2197328
display id: range2197328
version: 1
direction: direction/forward
top level: BBa_K864601
start: 1
end: 53
orientation: inline

BBa_K101000
type: ComponentDefinition
persistent identity: BBa K101000
display id: BBa_K101000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Dual-Repressed Promoter for p22 mnt and TetR
creator: Bennett Swiniarski
created: 2008-07-06T11:00:00Z
modified: 2015-05-08T01:08:41Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _205_
m user id: 0
owner id: 3363
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This part is a promoter that is repressed by both p22 mnt and TetR. It can be used to control the response of any gene that follows this promoter. It will be repressed by the presence of either p22 mnt or TetR.
mutable notes: TetR usually binds to two operator sites, one located upstream from -35 and one located between -35 and -10. However, p22 mnt also has two operator sites, one between -35 and -10 and another downstream from -10. Since each had an operator site in between -35 and -10, we had to eliminate the TetR operator site in order to allow the promoter to work. TetR was picked because it has a stronger binding than p22 mnt, and so would be more able to use only one site to bind.
mutable provenance: This part was designed by combining sequences from parts C0040 (TetR promoter) and C0072 (p22 mnt promoter).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K101000_sequence/1

BBa_K1614000
type: ComponentDefinition
persistent identity: BBa K1614000
display id: BBa_K1614000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: T7 promoter for expression of functional RNA
creator: Stefan Holderbach
created: 2015-09-13T11:00:00Z
modified: 2015-09-14T12:29:29Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2031_
m user id: 12380
owner id: 16422
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Test
mutable notes: None.
mutable provenance: T7 phage minimal promoter (consensus)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: T7 promoter
sequence: https://synbiohub.org/public/igem/BBa_K1614000_sequence/1

T7 promoter
type: SequenceAnnotation
persistent identity: annotation2454728
display id: annotation2454728
version: 1
top level: BBa_K1614000
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1614000/annotation2454728/range2454728/1

https://synbiohub.org/public/igem/BBa_K1614000/annotation2454728/range2454728/1
type: Range
persistent identity: range2454728
display id: range2454728
version: 1
direction: direction/forward
top level: BBa_K1614000
start: 1
end: 17
orientation: inline

BBa_K086023
type: ComponentDefinition
persistent identity: BBa K086023
display id: BBa_K086023
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: modified Lutz-Bujard LacO promoter,with alternative sigma factor σ28
creator: Gairik Sachdeva
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:08:36Z
discontinued: false
dominant: false
group u list: _197_
m user id: 0
owner id: 2578
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: modified Lutz-Bujard LacO promoter,with alternative sigma factor σ28
mutable notes: cleaned
mutable provenance: Lac O and Sigma 28 binding sites' conserved sequences
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: P28_2
sequence: https://synbiohub.org/public/igem/BBa_K086023_sequence/1

P28_2
type: SequenceAnnotation
persistent identity: annotation1995757
display id: annotation1995757
version: 1
top level: BBa_K086023
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K086023/annotation1995757/range1995757/1

https://synbiohub.org/public/igem/BBa_K086023/annotation1995757/range1995757/1
type: Range
persistent identity: range1995757
display id: range1995757
version: 1
direction: direction/forward
top level: BBa_K086023
start: 1
end: 55
orientation: inline

BBa_K266004
type: ComponentDefinition
persistent identity: BBa K266004
display id: BBa_K266004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Const Lac Inverter -> LasR
creator: UNAM FCIENCIAS-CINVESTAV IRA
created: 2009-10-12T11:00:00Z
modified: 2015-05-08T01:11:43Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _363_
m user id: 0
owner id: 1955
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: Constitutive promoter J23100 with Lac system inverter controlling the expression of LasR
mutable notes: None
mutable provenance: The biobricks BBa_J23100 and BBa_K266003 where used for build this biobrick
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Inverter
component: BBa_K266003
component: BBa_J23100
sequence annotation: BBa_J23100
sequence annotation: BBa_K266003
sequence: https://synbiohub.org/public/igem/BBa_K266004_sequence/1

BBa_K266003
type: Component
persistent identity: component2287713
display id: component2287713
version: 1
top level: BBa_K266004
access: public
definition: https://synbiohub.org/public/igem/BBa_K266003/1

BBa_J23100
type: Component
persistent identity: component2287678
display id: component2287678
version: 1
top level: BBa_K266004
access: public
definition: BBa_J23100

BBa_J23100
type: SequenceAnnotation
persistent identity: annotation2287678
display id: annotation2287678
version: 1
top level: BBa_K266004
location: https://synbiohub.org/public/igem/BBa_K266004/annotation2287678/range2287678/1
component: BBa_J23100

https://synbiohub.org/public/igem/BBa_K266004/annotation2287678/range2287678/1
type: Range
persistent identity: range2287678
display id: range2287678
version: 1
direction: direction/reverse
top level: BBa_K266004
start: 1
end: 35
orientation: inline

BBa_K266003
type: SequenceAnnotation
persistent identity: annotation2287713
display id: annotation2287713
version: 1
top level: BBa_K266004
location: https://synbiohub.org/public/igem/BBa_K266004/annotation2287713/range2287713/1
component: BBa_K266003

https://synbiohub.org/public/igem/BBa_K266004/annotation2287713/range2287713/1
type: Range
persistent identity: range2287713
display id: range2287713
version: 1
direction: direction/reverse
top level: BBa_K266004
start: 44
end: 2358
orientation: inline

BBa_K165000
type: ComponentDefinition
persistent identity: BBa K165000
display id: BBa_K165000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: MET 25 Promoter
creator: John Szymanski
created: 2008-10-24T11:00:00Z
modified: 2015-05-08T01:10:55Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _267_
m user id: 0
owner id: 2512
owning group id: 58
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: The is a repressable promoter for use in Saccharomyces cerevisiae. It is repressed by the presence of methionine. When unrepresssed, is has a moderate level of activity.
mutable notes: None.
mutable provenance: From the Saccharomyces cerevisiae genome, by way of Caroline Ajo-Franklin.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K165000_sequence/1

BBa_B1004
type: ComponentDefinition
persistent identity: BBa B1004
display id: BBa_B1004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Terminator (artificial, small, %T~=55)
creator: Haiyao Huang
created: 2006-08-30T11:00:00Z
modified: 2015-08-31T04:07:21Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _41_
m user id: 0
owner id: 745
owning group id: 41
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Artificial terminator, estimated %T~=55 * 6bp stem, 4nt loop * Bidirectional, estimated reverse %T~<20
mutable notes: Bidirectional, with the reverse estimated to be less effective than the forward. Has a polyA tail of 4 residues.
mutable provenance: antiquity
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: B1004
sequence annotation: PolyA
sequence annotation: stem loop
sequence: https://synbiohub.org/public/igem/BBa_B1004_sequence/1

B1004
type: SequenceAnnotation
persistent identity: annotation1898420
display id: annotation1898420
version: 1
top level: BBa_B1004
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_B1004/annotation1898420/range1898420/1

https://synbiohub.org/public/igem/BBa_B1004/annotation1898420/range1898420/1
type: Range
persistent identity: range1898420
display id: range1898420
version: 1
direction: direction/forward
top level: BBa_B1004
start: 1
end: 34
orientation: inline

PolyA
type: SequenceAnnotation
persistent identity: annotation1898422
display id: annotation1898422
version: 1
top level: BBa_B1004
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_B1004/annotation1898422/range1898422/1

https://synbiohub.org/public/igem/BBa_B1004/annotation1898422/range1898422/1
type: Range
persistent identity: range1898422
display id: range1898422
version: 1
direction: direction/reverse
top level: BBa_B1004
start: 6
end: 9
orientation: inline

PolyA
type: SequenceAnnotation
persistent identity: annotation1898423
display id: annotation1898423
version: 1
top level: BBa_B1004
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_B1004/annotation1898423/range1898423/1

https://synbiohub.org/public/igem/BBa_B1004/annotation1898423/range1898423/1
type: Range
persistent identity: range1898423
display id: range1898423
version: 1
direction: direction/forward
top level: BBa_B1004
start: 26
end: 29
orientation: inline

stem loop
type: SequenceAnnotation
persistent identity: annotation1898421
display id: annotation1898421
version: 1
top level: BBa_B1004
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_B1004/annotation1898421/range1898421/1

https://synbiohub.org/public/igem/BBa_B1004/annotation1898421/range1898421/1
type: Range
persistent identity: range1898421
display id: range1898421
version: 1
direction: direction/forward
top level: BBa_B1004
start: 10
end: 25
orientation: inline

BBa_K105027
type: ComponentDefinition
persistent identity: BBa K105027
display id: BBa_K105027
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: cyc100 minimal promoter
creator: Michael Wild
created: 2008-10-25T11:00:00Z
modified: 2015-05-08T01:08:52Z
discontinued: false
dominant: false
experience: experience/None
group u list: _253_
m user id: 0
owner id: 3313
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This is the core of the CYC1 promoter of ''S. cerevisiae''. This promoter element allows basal transcription. The transcription signal can be modulated by adding operator sites upstream of this BioBrick. In that way activators or repressors can act on the transcription machinery. <br<
mutable notes: This BioBrick contains the TATA-box and most of the 5'UTR (-139 to -36, relative to the translational start)of the natural CYC1 promoter. A Kozak sequence is not included and has thus to be added!
mutable provenance: Amplification from the plasmid pAH3.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: TATA box
sequence: https://synbiohub.org/public/igem/BBa_K105027_sequence/1

TATA box
type: SequenceAnnotation
persistent identity: annotation1989774
display id: annotation1989774
version: 1
top level: BBa_K105027
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K105027/annotation1989774/range1989774/1

https://synbiohub.org/public/igem/BBa_K105027/annotation1989774/range1989774/1
type: Range
persistent identity: range1989774
display id: range1989774
version: 1
direction: direction/forward
top level: BBa_K105027
start: 17
end: 23
orientation: inline

BBa_K086022
type: ComponentDefinition
persistent identity: BBa K086022
display id: BBa_K086022
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: modified Lutz-Bujard LacO promoter,with alternative sigma factor σ28
creator: Gairik Sachdeva
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:08:36Z
discontinued: false
dominant: false
group u list: _197_
m user id: 0
owner id: 2578
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: modified Lutz-Bujard LacO promoter,with alternative sigma factor σ28
mutable notes: Cleaned
mutable provenance: Lac and sigma 28 conserved binding sites
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: P28_1
sequence: https://synbiohub.org/public/igem/BBa_K086022_sequence/1

P28_1
type: SequenceAnnotation
persistent identity: annotation1995756
display id: annotation1995756
version: 1
top level: BBa_K086022
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K086022/annotation1995756/range1995756/1

https://synbiohub.org/public/igem/BBa_K086022/annotation1995756/range1995756/1
type: Range
persistent identity: range1995756
display id: range1995756
version: 1
direction: direction/forward
top level: BBa_K086022
start: 1
end: 55
orientation: inline

BBa_M13502
type: ComponentDefinition
persistent identity: BBa M13502
display id: BBa_M13502
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: M13KO7 gene II RBS
creator: Natalie Kuldell
created: 2006-12-14T12:00:00Z
modified: 2015-05-08T01:13:56Z
discontinued: false
dominant: false
experience: experience/None
group u list: _45_
m user id: 0
owner id: 314
owning group id: 1
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This RBS is part of the bacteriophage M13 genome, initiating translation of the gene II message (BBa_M13002). The part aligns with base pairs 8252-8267 in M13K07 genome. It was identified as the RBS for gene II message by Wezenbeek et al in Gene (1980) 11: 129-148
mutable notes: none
mutable provenance: New England Biolabs M13K07. Sequenced in 2002 by L. Panganaban
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_M13502_sequence/1

BBa_K259009
type: ComponentDefinition
persistent identity: BBa K259009
display id: BBa_K259009
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: BioScaffold Linker - Removes Stop Codons & scars & replaces with a Gly-Ser linker
creator: Petros Mina
created: 2009-10-12T11:00:00Z
modified: 2015-05-08T01:11:42Z
discontinued: false
dominant: false
group u list: _357_
m user id: 0
owner id: 5318
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part is part of a family of Bioscaffold - Linkers. This part will help users interested in protein-fusion. The usage of the part will allow in frame assembly of 2(+) proteins by: *Removing the stop codons from the upstream protein. *Removing the scar created by the Assembly Standard 10 (RFC10) construction of 2(+) protein coding sequences. *Maintaining the start codon of the downstream protein. *Adding a flexible (Gly-Ser-Gly) linker between the two protein coding sequences. *Acting as a translational buffer if ligation is incorrect. ===Assembly Instructions=== *Create an Assembly Standard 10 fusion as below ; **Upstream Protein Part - Bioscaffold Linker - Downstream Protein Part** *Use the BioScaffold Specific Enzymes (i)Restrict with BpuEI to remove upstream scar (created between Upstream Protein - Bioscaffold) and stop codons from upstream protein part. (ii)Ligate and you will get the intermediate product; **Upstream Protein (w/out Stop codons) - Tyr - BioScaffold Linker - Downstream Protein** *Restrict with CspCI to remove downstream scar. *Ligate to get the final product; **Upstream Protein - Tyr-Gly-Ser-Gly- Downstream Protein** ====N.B.==== CspCI cuts with a 1 b.p discrepancy depending on the sequence. However this should not affect the restriction/ligation process as this has been taken into account and incorporated into the design. Even with the discrepancy involved the restriction/ligation process will still give the same product and in-frame fusion. The other restriction variants of CspCI have also been taken into account and relevant BioScaffolds are being produced to be tested. This version is for when CspCI cuts 13/10b.p. (top/bottom) on the 5' of the recognition sequence and 10/13bp (top/bottom) on the 3' of the…
mutable notes: The CspCI discrepancy had to be taken into account
mutable provenance: This part is completely designed ''de novo''. This sequence does not occur naturally.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: BpuEI (rev. complement)
sequence annotation: Translational Buffer
sequence annotation: 5' CspCI Recognition sequence
sequence annotation: BpuEI
sequence annotation: Met involved in restriction/ligation
sequence annotation: 3' CspCI recognition sequence
sequence annotation: Gly-Ser-Gly linker
sequence annotation: Tyr involved in restriction/ligation
sequence: https://synbiohub.org/public/igem/BBa_K259009_sequence/1

BpuEI (rev. complement)
type: SequenceAnnotation
persistent identity: annotation2037400
display id: annotation2037400
version: 1
top level: BBa_K259009
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K259009/annotation2037400/range2037400/1

https://synbiohub.org/public/igem/BBa_K259009/annotation2037400/range2037400/1
type: Range
persistent identity: range2037400
display id: range2037400
version: 1
direction: direction/reverse
top level: BBa_K259009
start: 3
end: 8
orientation: inline

Translational Buffer
type: SequenceAnnotation
persistent identity: annotation2037439
display id: annotation2037439
version: 1
top level: BBa_K259009
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K259009/annotation2037439/range2037439/1

https://synbiohub.org/public/igem/BBa_K259009/annotation2037439/range2037439/1
type: Range
persistent identity: range2037439
display id: range2037439
version: 1
direction: direction/forward
top level: BBa_K259009
start: 69
end: 73
orientation: inline

5' CspCI Recognition sequence
type: SequenceAnnotation
persistent identity: annotation2037437
display id: annotation2037437
version: 1
top level: BBa_K259009
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K259009/annotation2037437/range2037437/1

https://synbiohub.org/public/igem/BBa_K259009/annotation2037437/range2037437/1
type: Range
persistent identity: range2037437
display id: range2037437
version: 1
direction: direction/forward
top level: BBa_K259009
start: 66
end: 68
orientation: inline

BpuEI
type: SequenceAnnotation
persistent identity: annotation2037401
display id: annotation2037401
version: 1
top level: BBa_K259009
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K259009/annotation2037401/range2037401/1

https://synbiohub.org/public/igem/BBa_K259009/annotation2037401/range2037401/1
type: Range
persistent identity: range2037401
display id: range2037401
version: 1
direction: direction/forward
top level: BBa_K259009
start: 21
end: 26
orientation: inline

Met involved in restriction/ligation
type: SequenceAnnotation
persistent identity: annotation2037404
display id: annotation2037404
version: 1
top level: BBa_K259009
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K259009/annotation2037404/range2037404/1

https://synbiohub.org/public/igem/BBa_K259009/annotation2037404/range2037404/1
type: Range
persistent identity: range2037404
display id: range2037404
version: 1
direction: direction/forward
top level: BBa_K259009
start: 53
end: 55
orientation: inline

3' CspCI recognition sequence
type: SequenceAnnotation
persistent identity: annotation2037438
display id: annotation2037438
version: 1
top level: BBa_K259009
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K259009/annotation2037438/range2037438/1

https://synbiohub.org/public/igem/BBa_K259009/annotation2037438/range2037438/1
type: Range
persistent identity: range2037438
display id: range2037438
version: 1
direction: direction/forward
top level: BBa_K259009
start: 74
end: 77
orientation: inline

Translational Buffer
type: SequenceAnnotation
persistent identity: annotation2037405
display id: annotation2037405
version: 1
top level: BBa_K259009
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K259009/annotation2037405/range2037405/1

https://synbiohub.org/public/igem/BBa_K259009/annotation2037405/range2037405/1
type: Range
persistent identity: range2037405
display id: range2037405
version: 1
direction: direction/forward
top level: BBa_K259009
start: 56
end: 65
orientation: inline

Gly-Ser-Gly linker
type: SequenceAnnotation
persistent identity: annotation2037403
display id: annotation2037403
version: 1
top level: BBa_K259009
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K259009/annotation2037403/range2037403/1

https://synbiohub.org/public/igem/BBa_K259009/annotation2037403/range2037403/1
type: Range
persistent identity: range2037403
display id: range2037403
version: 1
direction: direction/forward
top level: BBa_K259009
start: 44
end: 52
orientation: inline

Tyr involved in restriction/ligation
type: SequenceAnnotation
persistent identity: annotation2037402
display id: annotation2037402
version: 1
top level: BBa_K259009
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K259009/annotation2037402/range2037402/1

https://synbiohub.org/public/igem/BBa_K259009/annotation2037402/range2037402/1
type: Range
persistent identity: range2037402
display id: range2037402
version: 1
direction: direction/forward
top level: BBa_K259009
start: 41
end: 43
orientation: inline

BBa_B0031
type: ComponentDefinition
persistent identity: BBa B0031
display id: BBa_B0031
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS.2 (weak) -- derivative of BBa_0030
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _41_44_48_46_1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Medium RBS based on Ron Weiss thesis. Strength considered relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>.
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("RBS-1" in figure 4-14 of thesis). No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Cho
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0031_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23316
display id: annotation23316
version: 1
top level: BBa_B0031
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_B0031/annotation23316/range23316/1

https://synbiohub.org/public/igem/BBa_B0031/annotation23316/range23316/1
type: Range
persistent identity: range23316
display id: range23316
version: 1
direction: direction/forward
top level: BBa_B0031
start: 7
end: 10
orientation: inline

BBa_K1392970
type: ComponentDefinition
persistent identity: BBa K1392970
display id: BBa_K1392970
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Terminator+Tetr Promoter+T4 Endolysin
creator: Şeniz Y??ksel
created: 2014-10-09T11:00:00Z
modified: 2015-05-08T01:10:15Z
discontinued: false
dominant: false
group u list: _1769_
m user id: 0
owner id: 16902
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: For this part an artifical Terminator is used (generally added to another parts). The promoter is a TetR repressible promoter.It is sequenced for pTet inverting regulator. Promoter is constitutively ON and repressed by TetR. TetR repression is inhibited by the addition of tetracycline or its analog part
mutable notes: Bidirectional, with the reverse estimated to be more effective than the forward. Has a polyA tail of 9 residues. High expression of lysozyme only is toxic to bacteria. BBa_R0040 (TetR repressible promoter) is based on a cI promoter. It has been modified to include two TetR binding sites. Constructed by DNA synthesis.
mutable provenance: Enterobacteria phage T4 http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?val=29366675&from=66503&to=66997&view=gbwithparts modified E. coli thr terminator, replaced all A-T pairs in stem with C-G pairs BiblioPlus Extension Error fetching PMID 9092630:
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K112806
component: BBa_B1006
component: BBa_R0040
sequence annotation: BBa_K112806
sequence annotation: BBa_R0040
sequence annotation: BBa_B1006
sequence: https://synbiohub.org/public/igem/BBa_K1392970_sequence/1

BBa_K112806
type: Component
persistent identity: component2415808
display id: component2415808
version: 1
top level: BBa_K1392970
access: public
definition: https://synbiohub.org/public/igem/BBa_K112806/1

BBa_B1006
type: Component
persistent identity: component2415801
display id: component2415801
version: 1
top level: BBa_K1392970
access: public
definition: BBa_B1006

BBa_R0040
type: Component
persistent identity: component2415802
display id: component2415802
version: 1
top level: BBa_K1392970
access: public
definition: p(tetR)

BBa_K112806
type: SequenceAnnotation
persistent identity: annotation2415808
display id: annotation2415808
version: 1
top level: BBa_K1392970
location: https://synbiohub.org/public/igem/BBa_K1392970/annotation2415808/range2415808/1
component: BBa_K112806

https://synbiohub.org/public/igem/BBa_K1392970/annotation2415808/range2415808/1
type: Range
persistent identity: range2415808
display id: range2415808
version: 1
direction: direction/reverse
top level: BBa_K1392970
start: 110
end: 623
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation2415802
display id: annotation2415802
version: 1
top level: BBa_K1392970
location: https://synbiohub.org/public/igem/BBa_K1392970/annotation2415802/range2415802/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_K1392970/annotation2415802/range2415802/1
type: Range
persistent identity: range2415802
display id: range2415802
version: 1
direction: direction/forward
top level: BBa_K1392970
start: 48
end: 101
orientation: inline

BBa_B1006
type: SequenceAnnotation
persistent identity: annotation2415801
display id: annotation2415801
version: 1
top level: BBa_K1392970
location: https://synbiohub.org/public/igem/BBa_K1392970/annotation2415801/range2415801/1
component: BBa_B1006

https://synbiohub.org/public/igem/BBa_K1392970/annotation2415801/range2415801/1
type: Range
persistent identity: range2415801
display id: range2415801
version: 1
direction: direction/reverse
top level: BBa_K1392970
start: 1
end: 39
orientation: inline

Pctc
type: ComponentDefinition
persistent identity: BBa K143010
display id: BBa_K143010
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter ctc for B. subtilis
creator: James Chappell
created: 2008-09-10T11:00:00Z
modified: 2015-05-08T01:10:23Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _199_
m user id: 0
owner id: 2090
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Promoter ctc is a sigma factor B dependent promoter found in ''B.subtilis''. In ''B.subtilis'' endogenous sigma factor B is activated under mild stress. These mild stress conditions can be generally split into nutrient stress response and physical stress response. Nutrient stress response is triggered by low levels of ATP and GTP and physical stress response is triggered by exposure to blue light, salt, heat, acid or ethanol1. The promoter ctc has been used previously as a read out for the activation of sigma factor B 2. *The context with which we used the promoter ctc, was to take blue light as an input and give '''Polymerase Per Second'''(PoPS) as an output. To do this the other potential inputs need to be carefully controlled so that only blue light activated the sigma B and gives a PoPS output. In order to get sufficient sigma B activation by blue light the light receptor YtvA, part...., needs to be over expressed in ''B.subtilis'' 3. ===Reference=== #1 pmid=16267279 #2 pmid=3100810 #3 pmid=17575448
mutable notes: Biobrick standard was applied to the promoter ctc sequence.
mutable provenance: The part was designed using the sequence from the ''B.subtilis'' genome and from previously published papers 23. This sequence was then synthesised by Geneart.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: Sigma B -10
sequence annotation: Sigma B -35
sequence: https://synbiohub.org/public/igem/BBa_K143010_sequence/1

Sigma B -10
type: SequenceAnnotation
persistent identity: annotation1975700
display id: annotation1975700
version: 1
top level: Pctc
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K143010/annotation1975700/range1975700/1

https://synbiohub.org/public/igem/BBa_K143010/annotation1975700/range1975700/1
type: Range
persistent identity: range1975700
display id: range1975700
version: 1
direction: direction/forward
top level: Pctc
start: 41
end: 46
orientation: inline

Sigma B -35
type: SequenceAnnotation
persistent identity: annotation1975701
display id: annotation1975701
version: 1
top level: Pctc
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K143010/annotation1975701/range1975701/1

https://synbiohub.org/public/igem/BBa_K143010/annotation1975701/range1975701/1
type: Range
persistent identity: range1975701
display id: range1975701
version: 1
direction: direction/forward
top level: Pctc
start: 21
end: 28
orientation: inline

BBa_K1486011
type: ComponentDefinition
persistent identity: BBa K1486011
display id: BBa_K1486011
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CxpR & Split IFP1.4 [Cterm + Nterm]
creator: iGem EPFL 2014
created: 2014-08-26T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part has two fused proteins downstream of an arabinose-inducible promoter: CpxR-IFP[1] and CpxR-IFP[2]. This construct was made in all orientations: - CpxR-IFP[1] + CpxR-IFP[2] (BBa_K1286008) - IFP[1]-CpxR + CpxR-IFP[2] (BBa_K1486010) - CpxR-IFP[1] + IFP[2]-CpxR (BBa_K1486011)
mutable notes: All orientations were made, so all dimerizations could be tested (see main page for biobrick numbers). Flexible linkers were used for each of the fused proteins, and an RBS was put before each one of them.
mutable provenance: From Michnick Article. To Edit
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K1486007
component: BBa_B0034
component: BBa_K1486000
component: BBa_I0500
component: BBa_K1486004
component: BBa_K1486006
sequence annotation: BBa_I0500
sequence annotation: BBa_K1486006
sequence annotation: BBa_K1486000
sequence annotation: BBa_K1486007
sequence annotation: BBa_K1486004
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K1486011_sequence/1

BBa_K1486007
type: Component
persistent identity: component2382249
display id: component2382249
version: 1
top level: BBa_K1486011
access: public
definition: BBa_K1486007

BBa_B0034
type: Component
persistent identity: component2382237
display id: component2382237
version: 1
top level: BBa_K1486011
access: public
definition: BBa_B0034

BBa_K1486000
type: Component
persistent identity: component2382254
display id: component2382254
version: 1
top level: BBa_K1486011
access: public
definition: BBa_K1486000

BBa_K1486000
type: Component
persistent identity: component2382241
display id: component2382241
version: 1
top level: BBa_K1486011
access: public
definition: BBa_K1486000

BBa_I0500
type: Component
persistent identity: component2382235
display id: component2382235
version: 1
top level: BBa_K1486011
access: public
definition: pBad/araC

BBa_K1486004
type: Component
persistent identity: component2382242
display id: component2382242
version: 1
top level: BBa_K1486011
access: public
definition: BBa_K1486004

BBa_K1486004
type: Component
persistent identity: component2382250
display id: component2382250
version: 1
top level: BBa_K1486011
access: public
definition: BBa_K1486004

BBa_K1486006
type: Component
persistent identity: component2382243
display id: component2382243
version: 1
top level: BBa_K1486011
access: public
definition: BBa_K1486006

BBa_B0034
type: Component
persistent identity: component2382245
display id: component2382245
version: 1
top level: BBa_K1486011
access: public
definition: BBa_B0034

BBa_I0500
type: SequenceAnnotation
persistent identity: annotation2382235
display id: annotation2382235
version: 1
top level: BBa_K1486011
location: https://synbiohub.org/public/igem/BBa_K1486011/annotation2382235/range2382235/1
component: BBa_I0500

https://synbiohub.org/public/igem/BBa_K1486011/annotation2382235/range2382235/1
type: Range
persistent identity: range2382235
display id: range2382235
version: 1
direction: direction/reverse
top level: BBa_K1486011
start: 1
end: 1210
orientation: inline

BBa_K1486006
type: SequenceAnnotation
persistent identity: annotation2382243
display id: annotation2382243
version: 1
top level: BBa_K1486011
location: https://synbiohub.org/public/igem/BBa_K1486011/annotation2382243/range2382243/1
component: BBa_K1486006

https://synbiohub.org/public/igem/BBa_K1486011/annotation2382243/range2382243/1
type: Range
persistent identity: range2382243
display id: range2382243
version: 1
direction: direction/reverse
top level: BBa_K1486011
start: 1955
end: 2536
orientation: inline

BBa_K1486000
type: SequenceAnnotation
persistent identity: annotation2382254
display id: annotation2382254
version: 1
top level: BBa_K1486011
location: https://synbiohub.org/public/igem/BBa_K1486011/annotation2382254/range2382254/1
component: BBa_K1486000

https://synbiohub.org/public/igem/BBa_K1486011/annotation2382254/range2382254/1
type: Range
persistent identity: range2382254
display id: range2382254
version: 1
direction: direction/reverse
top level: BBa_K1486011
start: 3158
end: 3859
orientation: inline

BBa_K1486000
type: SequenceAnnotation
persistent identity: annotation2382241
display id: annotation2382241
version: 1
top level: BBa_K1486011
location: https://synbiohub.org/public/igem/BBa_K1486011/annotation2382241/range2382241/1
component: BBa_K1486000

https://synbiohub.org/public/igem/BBa_K1486011/annotation2382241/range2382241/1
type: Range
persistent identity: range2382241
display id: range2382241
version: 1
direction: direction/reverse
top level: BBa_K1486011
start: 1223
end: 1924
orientation: inline

BBa_K1486007
type: SequenceAnnotation
persistent identity: annotation2382249
display id: annotation2382249
version: 1
top level: BBa_K1486011
location: https://synbiohub.org/public/igem/BBa_K1486011/annotation2382249/range2382249/1
component: BBa_K1486007

https://synbiohub.org/public/igem/BBa_K1486011/annotation2382249/range2382249/1
type: Range
persistent identity: range2382249
display id: range2382249
version: 1
direction: direction/reverse
top level: BBa_K1486011
start: 2549
end: 3127
orientation: inline

BBa_K1486004
type: SequenceAnnotation
persistent identity: annotation2382250
display id: annotation2382250
version: 1
top level: BBa_K1486011
location: https://synbiohub.org/public/igem/BBa_K1486011/annotation2382250/range2382250/1
component: BBa_K1486004

https://synbiohub.org/public/igem/BBa_K1486011/annotation2382250/range2382250/1
type: Range
persistent identity: range2382250
display id: range2382250
version: 1
direction: direction/reverse
top level: BBa_K1486011
start: 3128
end: 3157
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2382237
display id: annotation2382237
version: 1
top level: BBa_K1486011
location: https://synbiohub.org/public/igem/BBa_K1486011/annotation2382237/range2382237/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1486011/annotation2382237/range2382237/1
type: Range
persistent identity: range2382237
display id: range2382237
version: 1
direction: direction/reverse
top level: BBa_K1486011
start: 1211
end: 1222
orientation: inline

BBa_K1486004
type: SequenceAnnotation
persistent identity: annotation2382242
display id: annotation2382242
version: 1
top level: BBa_K1486011
location: https://synbiohub.org/public/igem/BBa_K1486011/annotation2382242/range2382242/1
component: BBa_K1486004

https://synbiohub.org/public/igem/BBa_K1486011/annotation2382242/range2382242/1
type: Range
persistent identity: range2382242
display id: range2382242
version: 1
direction: direction/reverse
top level: BBa_K1486011
start: 1925
end: 1954
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2382245
display id: annotation2382245
version: 1
top level: BBa_K1486011
location: https://synbiohub.org/public/igem/BBa_K1486011/annotation2382245/range2382245/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1486011/annotation2382245/range2382245/1
type: Range
persistent identity: range2382245
display id: range2382245
version: 1
direction: direction/reverse
top level: BBa_K1486011
start: 2537
end: 2548
orientation: inline

U049O11+D0
type: ComponentDefinition
persistent identity: BBa I732438
display id: BBa_I732438
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Family Member (U049O11+D014O22)
creator: Zhan Jian
created: 2007-10-13T11:00:00Z
modified: 2015-08-31T04:07:58Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732438_sequence/1

BBa_K1520005
type: ComponentDefinition
persistent identity: BBa K1520005
display id: BBa_K1520005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Plac-rbs-rfp-Ter
creator: yue wu
created: 2014-09-21T11:00:00Z
modified: 2015-05-08T01:10:48Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1902_
m user id: 0
owner id: 21318
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Plac-rbs-rfp-Ter It produce rfp when there is enough IPTG or less lacI
mutable notes: None
mutable provenance: Plac, rfp, Ter
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_K1520500
component: BBa_K1520029
component: BBa_R0010
sequence annotation: BBa_K1520029
sequence annotation: BBa_R0010
sequence annotation: BBa_K1520500
sequence: https://synbiohub.org/public/igem/BBa_K1520005_sequence/1

BBa_K1520500
type: Component
persistent identity: component2384906
display id: component2384906
version: 1
top level: BBa_K1520005
access: public
definition: https://synbiohub.org/public/igem/BBa_K1520500/1

BBa_K1520029
type: Component
persistent identity: component2384907
display id: component2384907
version: 1
top level: BBa_K1520005
access: public
definition: https://synbiohub.org/public/igem/BBa_K1520029/1

BBa_R0010
type: Component
persistent identity: component2384899
display id: component2384899
version: 1
top level: BBa_K1520005
access: public
definition: LacI

BBa_K1520029
type: SequenceAnnotation
persistent identity: annotation2384907
display id: annotation2384907
version: 1
top level: BBa_K1520005
location: https://synbiohub.org/public/igem/BBa_K1520005/annotation2384907/range2384907/1
component: BBa_K1520029

https://synbiohub.org/public/igem/BBa_K1520005/annotation2384907/range2384907/1
type: Range
persistent identity: range2384907
display id: range2384907
version: 1
direction: direction/reverse
top level: BBa_K1520005
start: 949
end: 1077
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation2384899
display id: annotation2384899
version: 1
top level: BBa_K1520005
location: https://synbiohub.org/public/igem/BBa_K1520005/annotation2384899/range2384899/1
component: BBa_R0010

https://synbiohub.org/public/igem/BBa_K1520005/annotation2384899/range2384899/1
type: Range
persistent identity: range2384899
display id: range2384899
version: 1
direction: direction/forward
top level: BBa_K1520005
start: 1
end: 200
orientation: inline

BBa_K1520500
type: SequenceAnnotation
persistent identity: annotation2384906
display id: annotation2384906
version: 1
top level: BBa_K1520005
location: https://synbiohub.org/public/igem/BBa_K1520005/annotation2384906/range2384906/1
component: BBa_K1520500

https://synbiohub.org/public/igem/BBa_K1520005/annotation2384906/range2384906/1
type: Range
persistent identity: range2384906
display id: range2384906
version: 1
direction: direction/reverse
top level: BBa_K1520005
start: 209
end: 940
orientation: inline

BBa_K1315005
type: ComponentDefinition
persistent identity: BBa K1315005
display id: BBa_K1315005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: The clp gene from Xanthomonas campestris
creator: Dimitrios Michailidis
created: 2014-09-24T11:00:00Z
modified: 2015-05-08T01:09:50Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1690_
m user id: 0
owner id: 21812
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: c-di-GMP Repressed DNA Binding Protein (Clp) Quorum sensing and bacterial cell signalling are mechanisms known to be utilised by bacteria to regulate their virulence. The RpfC-RpfG sensory system has been found to activate the production of virulence factors like extracellular enzymes and polysaccharides in Xanthomonas campestris. It has been also suggested that this system is involved in linking environmental stimuli, including DSF, with pathogenicity in Xanthomonas campestris. DSF is a cis-unsaturated fatty acid from the DSF family. The RpfC-RpfG two-component system is the most well understood one and is present in all Xanthomonads. http://www.ncbi.nlm.nih.gov/pubmed/?term=r+ryan+dsf+family http://www.ncbi.nlm.nih.gov/pubmed/?term=A+two-component+system+involving+an+HD-GYP+domain+protein+links+cell%E2%80%93cell+signalling+to+pathogenicity+gene+expression+in+Xanthomonas+campestris
mutable notes: The sequence had 1 PstI site that was mutated out by site-directed mutagenesis.
mutable provenance: The source of this part is Xanthomonas campestris pv. campestris 8004 genomic DNA which was provided by the Robert Ryan lab in the Molecular Microbiology department of the University of Dundee, UK.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Clp
sequence: https://synbiohub.org/public/igem/BBa_K1315005_sequence/1

Clp
type: SequenceAnnotation
persistent identity: annotation2386527
display id: annotation2386527
version: 1
top level: BBa_K1315005
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1315005/annotation2386527/range2386527/1

https://synbiohub.org/public/igem/BBa_K1315005/annotation2386527/range2386527/1
type: Range
persistent identity: range2386527
display id: range2386527
version: 1
direction: direction/forward
top level: BBa_K1315005
start: 1
end: 696
orientation: inline

BBa_K137032
type: ComponentDefinition
persistent identity: BBa K137032
display id: BBa_K137032
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: constitutive promoter with (C)12 between -10 and -35 elements
creator: Allen Lin
created: 2008-07-20T11:00:00Z
modified: 2015-05-08T01:10:09Z
discontinued: false
dominant: false
experience: experience/Issues
group u list: _187_
m user id: 0
owner id: 3112
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: constitutive promoter with (C)12 between -10 and -35 elements
mutable notes: With 12 C repeats between the -10 and -35 elements, the distance between the two elements is optimal for the sigma factor to bind to the promoter. Thus, the promoter should be in the 'on' state.
mutable provenance: This part was PCR amplified from two synthesized primers that bound to each other.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -10 element
sequence annotation: c repeat
sequence: https://synbiohub.org/public/igem/BBa_K137032_sequence/1

-10 element
type: SequenceAnnotation
persistent identity: annotation1967773
display id: annotation1967773
version: 1
top level: BBa_K137032
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K137032/annotation1967773/range1967773/1

https://synbiohub.org/public/igem/BBa_K137032/annotation1967773/range1967773/1
type: Range
persistent identity: range1967773
display id: range1967773
version: 1
direction: direction/forward
top level: BBa_K137032
start: 52
end: 57
orientation: inline

c repeat
type: SequenceAnnotation
persistent identity: annotation1967774
display id: annotation1967774
version: 1
top level: BBa_K137032
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K137032/annotation1967774/range1967774/1

https://synbiohub.org/public/igem/BBa_K137032/annotation1967774/range1967774/1
type: Range
persistent identity: range1967774
display id: range1967774
version: 1
direction: direction/forward
top level: BBa_K137032
start: 27
end: 38
orientation: inline

Prm +
type: ComponentDefinition
persistent identity: BBa I12007
display id: BBa_I12007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Modified lambda Prm promoter (OR-3 obliterated)
creator: Hans
created: 2004-07-14T11:00:00Z
modified: 2015-08-31T04:07:31Z
discontinued: false
dominant: true
experience: experience/None
group u list: _3_
m user id: 0
owner id: 103
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Lambda Prm promoter modified to be activated but not repressed by the lambda repressor (cI)
mutable notes: In wild type lambda phage, the OR3 site (-27 to -11) reads "tatcccttgcggtgata" on the sense strand of DNA. To prevent lambda repressor (cI) from binding to this site, the 4th through 10th nt of OR3 were replaced by the 7 nt between OR2 and OR1, "aaatagt" (-57 to -51), which in effect mutates 5 nt of the promoter. These nt were chosen to be about halfway between the -10 and -35 boxes of the promoter. Furthermore, since these nt already act as a spacer in wild-type phage, it is hoped they will not create undesired interactions.
mutable provenance: Shih and Gussin (1983)
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: OR2
sequence annotation: -10
sequence annotation: -35
sequence annotation: OR1
sequence annotation: mutate to obliterate OR3
sequence: https://synbiohub.org/public/igem/BBa_I12007_sequence/1

OR2
type: SequenceAnnotation
persistent identity: annotation937702
display id: annotation937702
version: 1
top level: Prm +
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I12007/annotation937702/range937702/1

https://synbiohub.org/public/igem/BBa_I12007/annotation937702/range937702/1
type: Range
persistent identity: range937702
display id: range937702
version: 1
direction: direction/forward
top level: Prm +
start: 33
end: 49
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation937705
display id: annotation937705
version: 1
top level: Prm +
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I12007/annotation937705/range937705/1

https://synbiohub.org/public/igem/BBa_I12007/annotation937705/range937705/1
type: Range
persistent identity: range937705
display id: range937705
version: 1
direction: direction/forward
top level: Prm +
start: 71
end: 76
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation937703
display id: annotation937703
version: 1
top level: Prm +
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I12007/annotation937703/range937703/1

https://synbiohub.org/public/igem/BBa_I12007/annotation937703/range937703/1
type: Range
persistent identity: range937703
display id: range937703
version: 1
direction: direction/forward
top level: Prm +
start: 48
end: 53
orientation: inline

OR1
type: SequenceAnnotation
persistent identity: annotation937701
display id: annotation937701
version: 1
top level: Prm +
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I12007/annotation937701/range937701/1

https://synbiohub.org/public/igem/BBa_I12007/annotation937701/range937701/1
type: Range
persistent identity: range937701
display id: range937701
version: 1
direction: direction/forward
top level: Prm +
start: 9
end: 25
orientation: inline

mutate to obliterate OR3
type: SequenceAnnotation
persistent identity: annotation937704
display id: annotation937704
version: 1
top level: Prm +
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_I12007/annotation937704/range937704/1

https://synbiohub.org/public/igem/BBa_I12007/annotation937704/range937704/1
type: Range
persistent identity: range937704
display id: range937704
version: 1
direction: direction/forward
top level: Prm +
start: 59
end: 65
orientation: inline

BBa_M13503
type: ComponentDefinition
persistent identity: BBa M13503
display id: BBa_M13503
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: M13K07 gene III RBS
creator: Natalie Kuldell
created: 2006-12-14T12:00:00Z
modified: 2015-05-08T01:13:56Z
discontinued: false
dominant: false
experience: experience/None
group u list: _45_
m user id: 0
owner id: 314
owning group id: 1
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This RBS is part of the bacteriophage M13 genome, initiating translation of the gene III message (BBa_M13003). The part aligns with base pairs 1563-1578 in M13K07 genome. It was identified as the RBS for gene III message by Wezenbeek et al in Gene (1980) 11: 129-148 New England Biolabs M13K07. Sequenced in 2002 by L. Panganaban
mutable notes: none
mutable provenance: New England Biolabs M13K07. Sequenced in 2002 by L. Panganaban
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_M13503_sequence/1

BBa_K117002
type: ComponentDefinition
persistent identity: BBa K117002
display id: BBa_K117002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LsrA promoter (indirectly activated by AI-2)
creator: Nguyen Xuan Hung
created: 2008-10-06T11:00:00Z
modified: 2015-05-08T01:09:34Z
discontinued: false
dominant: false
experience: experience/None
group u list: _209_
m user id: 0
owner id: 2774
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: The regulatory network for the uptake of Escherichia coli autoinducer 2 (AI-2) is comprised of a transporter complex, LsrABCD; its repressor, LsrR; and a cognate signal kinase, LsrK. This network is an integral part of the AI-2 quorum-sensing (QS) system. Once uptaken into the cell, AI-2 will be phosphorylated under the effect of LsrK. Without the presence of AI-2, LsrA promoter is inhibited by the repressor, LsrR. Phosphorylated AI-2 will prevent LsrR from inhibiting this promoter, hence activating it so that the downstream sequence can be expressed. To prevent the cell from self-inducing this promoter, we must suppress its AI-2 producing capability. It can be done by knocking out LuxS, one of the vital gene required for producing AI-2. By doing that, LsrA promoter can only be activated under the presence of AI-2 from other sources than the host cell which is LuxS mutant (say, by contact with normal cells). More information on AI-2 quorum-sensing (QS) system please refer to: http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=1952038
mutable notes: to be updated
mutable provenance: to be updated
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: RBS BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K117002_sequence/1

RBS BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1979372
display id: annotation1979372
version: 1
top level: BBa_K117002
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K117002/annotation1979372/range1979372/1

https://synbiohub.org/public/igem/BBa_K117002/annotation1979372/range1979372/1
type: Range
persistent identity: range1979372
display id: range1979372
version: 1
direction: direction/forward
top level: BBa_K117002
start: 112
end: 123
orientation: inline

BBa_I766558
type: ComponentDefinition
persistent identity: BBa I766558
display id: BBa_I766558
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pFig1 (Inducible) Promoter
creator: Nili Sommovilla
created: 2007-08-15T11:00:00Z
modified: 2015-08-31T04:08:10Z
discontinued: false
dominant: false
experience: experience/None
group u list: _155_
m user id: 0
owner id: 1931
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Inducible promoter in yeast
mutable notes: none
mutable provenance: endogenous promoter for Fig1 (Factor Induced Gene 1)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I766558_sequence/1

BBa_J22126
type: ComponentDefinition
persistent identity: BBa J22126
display id: BBa_J22126
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Rec A (SOS) promoter
creator: Krithiga Sankaran
created: 2006-09-05T11:00:00Z
modified: 2015-08-31T04:08:38Z
discontinued: false
dominant: false
group u list: _70_
m user id: 0
owner id: 1038
owning group id: 70
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: RecA (SOS)promoter.
mutable notes: The Rec A(SOS) promoter has been PCR amplified from the chromosomal DNA from MG1655(Ecoli).
mutable provenance: NA
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J22126_sequence/1

BBa_J64026
type: ComponentDefinition
persistent identity: BBa J64026
display id: BBa_J64026
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: sigE RBS
creator: Dan Widmaier
created: 2008-01-16T12:00:00Z
modified: 2015-08-31T01:56:26Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _98_
m user id: 0
owner id: 2407
owning group id: 98
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is the natural RBS for the sigE chaperone (BBa_J64025) in Salmonella Typhimurium LT2.
mutable notes: This part was not designed with compatibility to biobrick standard assembly in mind. This RBS has not been explicitly assayed for expression rate.
mutable provenance: Salmonella LT2 Genomic DNA
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: sigE RBS
sequence: https://synbiohub.org/public/igem/BBa_J64026_sequence/1

sigE RBS
type: SequenceAnnotation
persistent identity: annotation1959625
display id: annotation1959625
version: 1
top level: BBa_J64026
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_J64026/annotation1959625/range1959625/1

https://synbiohub.org/public/igem/BBa_J64026/annotation1959625/range1959625/1
type: Range
persistent identity: range1959625
display id: range1959625
version: 1
direction: direction/forward
top level: BBa_J64026
start: 1
end: 18
orientation: inline

BBa_B1005
type: ComponentDefinition
persistent identity: BBa B1005
display id: BBa_B1005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Terminator (artificial, small, %T~=25%
creator: Haiyao Huang
created: 2006-08-30T11:00:00Z
modified: 2015-08-31T04:07:21Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _41_
m user id: 0
owner id: 745
owning group id: 41
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Artificial terminator, estimated %T~=25% * 6bp stem, 4nt loop * Bidirectional, estimated reverse %T~<10%
mutable notes: Bidirectional, with the reverse estimated to be less effective than the forward. Has a polyA tail of 3 residues. Designed to be a terminator with low efficiency.
mutable provenance: antiquity
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: B1005
sequence annotation: stem loop
sequence annotation: PolyA
sequence: https://synbiohub.org/public/igem/BBa_B1005_sequence/1

B1005
type: SequenceAnnotation
persistent identity: annotation1898424
display id: annotation1898424
version: 1
top level: BBa_B1005
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_B1005/annotation1898424/range1898424/1

https://synbiohub.org/public/igem/BBa_B1005/annotation1898424/range1898424/1
type: Range
persistent identity: range1898424
display id: range1898424
version: 1
direction: direction/forward
top level: BBa_B1005
start: 1
end: 34
orientation: inline

stem loop
type: SequenceAnnotation
persistent identity: annotation1898426
display id: annotation1898426
version: 1
top level: BBa_B1005
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_B1005/annotation1898426/range1898426/1

https://synbiohub.org/public/igem/BBa_B1005/annotation1898426/range1898426/1
type: Range
persistent identity: range1898426
display id: range1898426
version: 1
direction: direction/forward
top level: BBa_B1005
start: 10
end: 25
orientation: inline

PolyA
type: SequenceAnnotation
persistent identity: annotation1898427
display id: annotation1898427
version: 1
top level: BBa_B1005
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_B1005/annotation1898427/range1898427/1

https://synbiohub.org/public/igem/BBa_B1005/annotation1898427/range1898427/1
type: Range
persistent identity: range1898427
display id: range1898427
version: 1
direction: direction/reverse
top level: BBa_B1005
start: 7
end: 9
orientation: inline

PolyA
type: SequenceAnnotation
persistent identity: annotation1898425
display id: annotation1898425
version: 1
top level: BBa_B1005
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_B1005/annotation1898425/range1898425/1

https://synbiohub.org/public/igem/BBa_B1005/annotation1898425/range1898425/1
type: Range
persistent identity: range1898425
display id: range1898425
version: 1
direction: direction/forward
top level: BBa_B1005
start: 26
end: 28
orientation: inline

BBa_K121011
type: ComponentDefinition
persistent identity: BBa K121011
display id: BBa_K121011
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: promoter (lacI regulated)
creator: Masashi Kajita
created: 2008-10-15T11:00:00Z
modified: 2015-05-08T01:09:41Z
discontinued: false
dominant: false
group u list: _220_
m user id: 0
owner id: 3180
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: pressure inducible lac promoter
mutable notes: na
mutable provenance: na
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: K121011
sequence annotation: -10
sequence annotation: CAP binding site
sequence annotation: LacI binding site
sequence annotation: -35
sequence: https://synbiohub.org/public/igem/BBa_K121011_sequence/1

K121011
type: SequenceAnnotation
persistent identity: annotation1981858
display id: annotation1981858
version: 1
top level: BBa_K121011
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K121011/annotation1981858/range1981858/1

https://synbiohub.org/public/igem/BBa_K121011/annotation1981858/range1981858/1
type: Range
persistent identity: range1981858
display id: range1981858
version: 1
direction: direction/forward
top level: BBa_K121011
start: 1
end: 232
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1981861
display id: annotation1981861
version: 1
top level: BBa_K121011
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K121011/annotation1981861/range1981861/1

https://synbiohub.org/public/igem/BBa_K121011/annotation1981861/range1981861/1
type: Range
persistent identity: range1981861
display id: range1981861
version: 1
direction: direction/forward
top level: BBa_K121011
start: 165
end: 168
orientation: inline

CAP binding site
type: SequenceAnnotation
persistent identity: annotation1981859
display id: annotation1981859
version: 1
top level: BBa_K121011
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K121011/annotation1981859/range1981859/1

https://synbiohub.org/public/igem/BBa_K121011/annotation1981859/range1981859/1
type: Range
persistent identity: range1981859
display id: range1981859
version: 1
direction: direction/forward
top level: BBa_K121011
start: 94
end: 131
orientation: inline

LacI binding site
type: SequenceAnnotation
persistent identity: annotation1981862
display id: annotation1981862
version: 1
top level: BBa_K121011
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K121011/annotation1981862/range1981862/1

https://synbiohub.org/public/igem/BBa_K121011/annotation1981862/range1981862/1
type: Range
persistent identity: range1981862
display id: range1981862
version: 1
direction: direction/forward
top level: BBa_K121011
start: 169
end: 203
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1981860
display id: annotation1981860
version: 1
top level: BBa_K121011
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K121011/annotation1981860/range1981860/1

https://synbiohub.org/public/igem/BBa_K121011/annotation1981860/range1981860/1
type: Range
persistent identity: range1981860
display id: range1981860
version: 1
direction: direction/forward
top level: BBa_K121011
start: 141
end: 146
orientation: inline

BBa_K1520010
type: ComponentDefinition
persistent identity: BBa K1520010
display id: BBa_K1520010
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Prlux-rbs-rfp-Ter
creator: yue wu
created: 2014-09-21T11:00:00Z
modified: 2015-05-08T01:10:49Z
discontinued: false
dominant: false
group u list: _1902_
m user id: 0
owner id: 21318
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Prlux-rbs-rfp-Ter. It produces rfp when there is enough luxR and AHL.
mutable notes: None
mutable provenance: Prlux, rfp, Ter
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_K1520029
component: BBa_R0062
component: BBa_K1520500
sequence annotation: BBa_K1520500
sequence annotation: BBa_K1520029
sequence annotation: BBa_R0062
sequence: https://synbiohub.org/public/igem/BBa_K1520010_sequence/1

BBa_K1520029
type: Component
persistent identity: component2384955
display id: component2384955
version: 1
top level: BBa_K1520010
access: public
definition: https://synbiohub.org/public/igem/BBa_K1520029/1

BBa_R0062
type: Component
persistent identity: component2384949
display id: component2384949
version: 1
top level: BBa_K1520010
access: public
definition: lux pR

BBa_K1520500
type: Component
persistent identity: component2384954
display id: component2384954
version: 1
top level: BBa_K1520010
access: public
definition: https://synbiohub.org/public/igem/BBa_K1520500/1

BBa_K1520500
type: SequenceAnnotation
persistent identity: annotation2384954
display id: annotation2384954
version: 1
top level: BBa_K1520010
location: https://synbiohub.org/public/igem/BBa_K1520010/annotation2384954/range2384954/1
component: BBa_K1520500

https://synbiohub.org/public/igem/BBa_K1520010/annotation2384954/range2384954/1
type: Range
persistent identity: range2384954
display id: range2384954
version: 1
direction: direction/reverse
top level: BBa_K1520010
start: 64
end: 795
orientation: inline

BBa_K1520029
type: SequenceAnnotation
persistent identity: annotation2384955
display id: annotation2384955
version: 1
top level: BBa_K1520010
location: https://synbiohub.org/public/igem/BBa_K1520010/annotation2384955/range2384955/1
component: BBa_K1520029

https://synbiohub.org/public/igem/BBa_K1520010/annotation2384955/range2384955/1
type: Range
persistent identity: range2384955
display id: range2384955
version: 1
direction: direction/reverse
top level: BBa_K1520010
start: 804
end: 932
orientation: inline

BBa_R0062
type: SequenceAnnotation
persistent identity: annotation2384949
display id: annotation2384949
version: 1
top level: BBa_K1520010
location: https://synbiohub.org/public/igem/BBa_K1520010/annotation2384949/range2384949/1
component: BBa_R0062

https://synbiohub.org/public/igem/BBa_K1520010/annotation2384949/range2384949/1
type: Range
persistent identity: range2384949
display id: range2384949
version: 1
direction: direction/forward
top level: BBa_K1520010
start: 1
end: 55
orientation: inline

BBa_I13406
type: ComponentDefinition
persistent identity: BBa I13406
display id: BBa_I13406
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Pbad/AraC with extra REN sites
creator: jkm
created: 2005-05-03T11:00:00Z
modified: 2015-08-31T04:07:33Z
discontinued: false
dominant: false
experience: experience/None
group u list: _6_
m user id: 0
owner id: 253
owning group id: 6
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: I0500 with extra trailing restriction sites: Eco-Xba-I0500-HindIII-NcoI-Spe-Pst
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I13406_sequence/1

hygR
type: ComponentDefinition
persistent identity: BBa K678042
display id: BBa_K678042
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: hygromycin cassette for Aspergillus nidulans
creator: DTU-Denmark-2
created: 2011-09-04T11:00:00Z
modified: 2015-05-08T01:13:04Z
discontinued: false
dominant: false
experience: experience/None
group u list: _882_
m user id: 0
owner id: 8341
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: hygromycin cassette for Aspergillus nidulans
mutable notes: Compatible with the Plug'n'Play assembly standard
mutable provenance: pCB1001
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: hygR cassette
sequence annotation: Linker 5
sequence: https://synbiohub.org/public/igem/BBa_K678042_sequence/1

hygR cassette
type: SequenceAnnotation
persistent identity: annotation2127416
display id: annotation2127416
version: 1
top level: hygR
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K678042/annotation2127416/range2127416/1

https://synbiohub.org/public/igem/BBa_K678042/annotation2127416/range2127416/1
type: Range
persistent identity: range2127416
display id: range2127416
version: 1
direction: direction/forward
top level: hygR
start: 9
end: 1363
orientation: inline

Linker 5
type: SequenceAnnotation
persistent identity: annotation2127415
display id: annotation2127415
version: 1
top level: hygR
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K678042/annotation2127415/range2127415/1

https://synbiohub.org/public/igem/BBa_K678042/annotation2127415/range2127415/1
type: Range
persistent identity: range2127415
display id: range2127415
version: 1
direction: direction/forward
top level: hygR
start: 1
end: 8
orientation: inline

BBa_K105031
type: ComponentDefinition
persistent identity: BBa K105031
display id: BBa_K105031
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: cyc16 minimal promoter
creator: Michael Wild
created: 2008-10-25T11:00:00Z
modified: 2015-05-08T01:08:52Z
discontinued: false
dominant: false
experience: experience/None
group u list: _253_
m user id: 0
owner id: 3313
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This is a derivate of BBa_K105027. Due to a point mutation in the TATA box region the efficiency of this minimal promoter is only 16 % of the natural yeast CYC1 promoter.
mutable notes: This BioBrick contains the TATA-box with one point mutation and most of the 5'UTR (-139 to -36, relative to the translational start) of the natural CYC1 promoter. A Kozak sequence is not included and has thus to be added! Even if there isn't any need for this BioBrick to be fusible to other protein domains, we stayed with our design.
mutable provenance: Amplification and Mutation from the plasmid pAH3
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: TATA-box
sequence annotation: A -> G
sequence: https://synbiohub.org/public/igem/BBa_K105031_sequence/1

TATA-box
type: SequenceAnnotation
persistent identity: annotation1989843
display id: annotation1989843
version: 1
top level: BBa_K105031
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K105031/annotation1989843/range1989843/1

https://synbiohub.org/public/igem/BBa_K105031/annotation1989843/range1989843/1
type: Range
persistent identity: range1989843
display id: range1989843
version: 1
direction: direction/forward
top level: BBa_K105031
start: 17
end: 23
orientation: inline

A -> G
type: SequenceAnnotation
persistent identity: annotation1989845
display id: annotation1989845
version: 1
top level: BBa_K105031
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_K105031/annotation1989845/range1989845/1

https://synbiohub.org/public/igem/BBa_K105031/annotation1989845/range1989845/1
type: Range
persistent identity: range1989845
display id: range1989845
version: 1
direction: direction/forward
top level: BBa_K105031
start: 22
end: 22
orientation: inline

BBa_K180007
type: ComponentDefinition
persistent identity: BBa K180007
display id: BBa_K180007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Game of Life - Secondary plasmid [tac pR, LuxI generator]
creator: Richard Wilson, Stephanie Chambers
created: 2009-10-15T11:00:00Z
modified: 2015-05-08T01:11:06Z
discontinued: false
dominant: false
experience: experience/None
group u list: _278_
m user id: 0
owner id: 4082
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: iGEM2009 Team:Southampton Game of Life secondary plasmid.
mutable notes: Regulated expression of LuxI (C0161) by TAC pR (K180000), a functional hybrid lac/trp promoter.
mutable provenance: See relevant basic biobrick parts (K180000, B0034, C0161, B0015).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_C0161
component: BBa_K180000
component: BBa_B0034
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_K180000
sequence annotation: BBa_B0034
sequence annotation: BBa_C0161
sequence annotation: BBa_B0010
sequence: https://synbiohub.org/public/igem/BBa_K180007_sequence/1

BBa_C0161
type: Component
persistent identity: component2046432
display id: component2046432
version: 1
top level: BBa_K180007
access: public
definition: https://synbiohub.org/public/igem/BBa_C0161/1

BBa_K180000
type: Component
persistent identity: component2046428
display id: component2046428
version: 1
top level: BBa_K180007
access: public
definition: BBa_K180000

BBa_B0034
type: Component
persistent identity: component2046430
display id: component2046430
version: 1
top level: BBa_K180007
access: public
definition: BBa_B0034

BBa_B0012
type: Component
persistent identity: component2046435
display id: component2046435
version: 1
top level: BBa_K180007
access: public
definition: BBa_B0012

BBa_B0010
type: Component
persistent identity: component2046433
display id: component2046433
version: 1
top level: BBa_K180007
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2046435
display id: annotation2046435
version: 1
top level: BBa_K180007
location: https://synbiohub.org/public/igem/BBa_K180007/annotation2046435/range2046435/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K180007/annotation2046435/range2046435/1
type: Range
persistent identity: range2046435
display id: range2046435
version: 1
direction: direction/forward
top level: BBa_K180007
start: 2155
end: 2195
orientation: inline

BBa_K180000
type: SequenceAnnotation
persistent identity: annotation2046428
display id: annotation2046428
version: 1
top level: BBa_K180007
location: https://synbiohub.org/public/igem/BBa_K180007/annotation2046428/range2046428/1
component: BBa_K180000

https://synbiohub.org/public/igem/BBa_K180007/annotation2046428/range2046428/1
type: Range
persistent identity: range2046428
display id: range2046428
version: 1
direction: direction/reverse
top level: BBa_K180007
start: 1
end: 1447
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2046430
display id: annotation2046430
version: 1
top level: BBa_K180007
location: https://synbiohub.org/public/igem/BBa_K180007/annotation2046430/range2046430/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K180007/annotation2046430/range2046430/1
type: Range
persistent identity: range2046430
display id: range2046430
version: 1
direction: direction/reverse
top level: BBa_K180007
start: 1456
end: 1467
orientation: inline

BBa_C0161
type: SequenceAnnotation
persistent identity: annotation2046432
display id: annotation2046432
version: 1
top level: BBa_K180007
location: https://synbiohub.org/public/igem/BBa_K180007/annotation2046432/range2046432/1
component: BBa_C0161

https://synbiohub.org/public/igem/BBa_K180007/annotation2046432/range2046432/1
type: Range
persistent identity: range2046432
display id: range2046432
version: 1
direction: direction/reverse
top level: BBa_K180007
start: 1474
end: 2058
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2046433
display id: annotation2046433
version: 1
top level: BBa_K180007
location: https://synbiohub.org/public/igem/BBa_K180007/annotation2046433/range2046433/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K180007/annotation2046433/range2046433/1
type: Range
persistent identity: range2046433
display id: range2046433
version: 1
direction: direction/forward
top level: BBa_K180007
start: 2067
end: 2146
orientation: inline

BBa_K121014
type: ComponentDefinition
persistent identity: BBa K121014
display id: BBa_K121014
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: promoter (lambda cI regulated)
creator: Masashi Kajita
created: 2008-10-15T11:00:00Z
modified: 2015-05-08T01:09:41Z
discontinued: false
dominant: false
group u list: _220_
m user id: 0
owner id: 3180
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: promoter (lambda cI regulated)
mutable notes: na
mutable provenance: na
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -10
sequence annotation: OL3
sequence annotation: K121014
sequence annotation: OL2
sequence annotation: -35
sequence annotation: OL1
sequence: https://synbiohub.org/public/igem/BBa_K121014_sequence/1

-10
type: SequenceAnnotation
persistent identity: annotation1981850
display id: annotation1981850
version: 1
top level: BBa_K121014
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K121014/annotation1981850/range1981850/1

https://synbiohub.org/public/igem/BBa_K121014/annotation1981850/range1981850/1
type: Range
persistent identity: range1981850
display id: range1981850
version: 1
direction: direction/forward
top level: BBa_K121014
start: 70
end: 75
orientation: inline

OL3
type: SequenceAnnotation
persistent identity: annotation1981847
display id: annotation1981847
version: 1
top level: BBa_K121014
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K121014/annotation1981847/range1981847/1

https://synbiohub.org/public/igem/BBa_K121014/annotation1981847/range1981847/1
type: Range
persistent identity: range1981847
display id: range1981847
version: 1
direction: direction/forward
top level: BBa_K121014
start: 13
end: 29
orientation: inline

K121014
type: SequenceAnnotation
persistent identity: annotation1981846
display id: annotation1981846
version: 1
top level: BBa_K121014
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K121014/annotation1981846/range1981846/1

https://synbiohub.org/public/igem/BBa_K121014/annotation1981846/range1981846/1
type: Range
persistent identity: range1981846
display id: range1981846
version: 1
direction: direction/forward
top level: BBa_K121014
start: 1
end: 90
orientation: inline

OL2
type: SequenceAnnotation
persistent identity: annotation1981848
display id: annotation1981848
version: 1
top level: BBa_K121014
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K121014/annotation1981848/range1981848/1

https://synbiohub.org/public/igem/BBa_K121014/annotation1981848/range1981848/1
type: Range
persistent identity: range1981848
display id: range1981848
version: 1
direction: direction/forward
top level: BBa_K121014
start: 33
end: 49
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1981851
display id: annotation1981851
version: 1
top level: BBa_K121014
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K121014/annotation1981851/range1981851/1

https://synbiohub.org/public/igem/BBa_K121014/annotation1981851/range1981851/1
type: Range
persistent identity: range1981851
display id: range1981851
version: 1
direction: direction/forward
top level: BBa_K121014
start: 47
end: 52
orientation: inline

OL1
type: SequenceAnnotation
persistent identity: annotation1981849
display id: annotation1981849
version: 1
top level: BBa_K121014
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K121014/annotation1981849/range1981849/1

https://synbiohub.org/public/igem/BBa_K121014/annotation1981849/range1981849/1
type: Range
persistent identity: range1981849
display id: range1981849
version: 1
direction: direction/forward
top level: BBa_K121014
start: 57
end: 73
orientation: inline

BBa_Z0261
type: ComponentDefinition
persistent identity: BBa Z0261
display id: BBa_Z0261
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Strong T7.2 RBS
creator: Sriram Kosuri
created: 2006-05-15T11:00:00Z
modified: 2015-05-08T01:14:55Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_10_
m user id: 0
owner id: 64
owning group id: 10
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Sequence comes from the natural gene 5 RBS. Using gene 5 as a strong RBS is from initial results from experiments characterizing all natural T7 RBSs.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_Z0261_sequence/1

BBa_J34806
type: ComponentDefinition
persistent identity: BBa J34806
display id: BBa_J34806
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: promoter lac induced
creator: Alexandra Choutko
created: 2006-10-25T11:00:00Z
modified: 2015-08-31T04:08:46Z
discontinued: false
dominant: false
experience: experience/None
group u list: _62_
m user id: 0
owner id: 1085
owning group id: 62
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: to be used with transcription factor BBa_J34802
mutable notes: a
mutable provenance: a
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J34806_sequence/1

BBa_K731201
type: ComponentDefinition
persistent identity: BBa K731201
display id: BBa_K731201
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Arabinose inducible araC-pBAD promoter
creator: Daniele Rossetto
created: 2012-08-02T11:00:00Z
modified: 2015-05-08T01:13:06Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _977_
m user id: 0
owner id: 11997
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: This E. coli promoter is positively regulated by arabinose. araC is the regulator protein, binding to arabinose prevents the repression of pBAD.
mutable notes: This part is similar to BBa_I0500, but it differs from that one in several point mutations in the sequence.
mutable provenance: E. coli genome
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: araC
sequence annotation: PC
sequence annotation: pBAD
sequence: https://synbiohub.org/public/igem/BBa_K731201_sequence/1

araC
type: SequenceAnnotation
persistent identity: annotation2179313
display id: annotation2179313
version: 1
top level: BBa_K731201
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K731201/annotation2179313/range2179313/1

https://synbiohub.org/public/igem/BBa_K731201/annotation2179313/range2179313/1
type: Range
persistent identity: range2179313
display id: range2179313
version: 1
direction: direction/reverse
top level: BBa_K731201
start: 1
end: 1080
orientation: inline

PC
type: SequenceAnnotation
persistent identity: annotation2179312
display id: annotation2179312
version: 1
top level: BBa_K731201
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K731201/annotation2179312/range2179312/1

https://synbiohub.org/public/igem/BBa_K731201/annotation2179312/range2179312/1
type: Range
persistent identity: range2179312
display id: range2179312
version: 1
direction: direction/reverse
top level: BBa_K731201
start: 1043
end: 1114
orientation: inline

pBAD
type: SequenceAnnotation
persistent identity: annotation2179311
display id: annotation2179311
version: 1
top level: BBa_K731201
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K731201/annotation2179311/range2179311/1

https://synbiohub.org/public/igem/BBa_K731201/annotation2179311/range2179311/1
type: Range
persistent identity: range2179311
display id: range2179311
version: 1
direction: direction/forward
top level: BBa_K731201
start: 1080
end: 1203
orientation: inline

BBa_J64807
type: ComponentDefinition
persistent identity: BBa J64807
display id: BBa_J64807
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RocD RBS
creator: David Stanley
created: 2007-03-19T12:00:00Z
modified: 2015-05-08T01:08:17Z
discontinued: false
dominant: false
experience: experience/None
group u list: _98_
m user id: 0
owner id: 1423
owning group id: 98
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: RocD RBS
mutable notes: none
mutable provenance: none
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J64807_sequence/1

BBa_K1486052
type: ComponentDefinition
persistent identity: BBa K1486052
display id: BBa_K1486052
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Spacer
creator: EPFL iGem team 2014
created: 2014-10-06T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: to edit
mutable notes: to edit
mutable provenance: to edit
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1486052_sequence/1

cI lam
type: ComponentDefinition
persistent identity: BBa R0051
display id: BBa_R0051
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: promoter (lambda cI regulated)
creator: Vinay S Mahajan, Brian Chow, Peter Carr, Voichita Marinescu and Alexander D. Wissner-Gross
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: The cI regulated promoter is based on the pR promtoer from bacteriohage lambda. The promoter has two two DNA binding sites for lambda cI repressor <bb_part>BBa_C0051</bb_part>. cI binding results in repression of transcription. The specific sequence used here is based on the cI repressible promoter used in the Elowitz repressilator (and references therein).
mutable notes: In order to address concerns about the promoter transcribing in the reverse direction, we have removed the -35 and -10 signals responsible for the promoter activity in the reverse direction. (More details needed here! DE, 2/24/03) Incompatible with host expressing cI repressor.
mutable provenance: A synthetic oscillatory network of transcriptional regulators , Elowitz M.B. , Leibler S., Nature(403),335-38: 2000
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: BBa_R0051
sequence annotation: -10
sequence annotation: OR1
sequence annotation: -35
sequence annotation: OR2
sequence: https://synbiohub.org/public/igem/BBa_R0051_sequence/1

BBa_R0051
type: SequenceAnnotation
persistent identity: annotation7067
display id: annotation7067
version: 1
top level: cI lam
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_R0051/annotation7067/range7067/1

https://synbiohub.org/public/igem/BBa_R0051/annotation7067/range7067/1
type: Range
persistent identity: range7067
display id: range7067
version: 1
direction: direction/forward
top level: cI lam
start: 1
end: 49
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2022
display id: annotation2022
version: 1
top level: cI lam
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0051/annotation2022/range2022/1

https://synbiohub.org/public/igem/BBa_R0051/annotation2022/range2022/1
type: Range
persistent identity: range2022
display id: range2022
version: 1
direction: direction/forward
top level: cI lam
start: 38
end: 43
orientation: inline

OR1
type: SequenceAnnotation
persistent identity: annotation2024
display id: annotation2024
version: 1
top level: cI lam
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0051/annotation2024/range2024/1

https://synbiohub.org/public/igem/BBa_R0051/annotation2024/range2024/1
type: Range
persistent identity: range2024
display id: range2024
version: 1
direction: direction/forward
top level: cI lam
start: 25
end: 41
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2023
display id: annotation2023
version: 1
top level: cI lam
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0051/annotation2023/range2023/1

https://synbiohub.org/public/igem/BBa_R0051/annotation2023/range2023/1
type: Range
persistent identity: range2023
display id: range2023
version: 1
direction: direction/forward
top level: cI lam
start: 15
end: 20
orientation: inline

OR2
type: SequenceAnnotation
persistent identity: annotation2025
display id: annotation2025
version: 1
top level: cI lam
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0051/annotation2025/range2025/1

https://synbiohub.org/public/igem/BBa_R0051/annotation2025/range2025/1
type: Range
persistent identity: range2025
display id: range2025
version: 1
direction: direction/forward
top level: cI lam
start: 1
end: 17
orientation: inline

Pnot(O33)
type: ComponentDefinition
persistent identity: BBa I732203
display id: BBa_I732203
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: NOT Gate Promoter Family Member (D001O33)
creator: Zhan Jian
created: 2007-10-13T11:00:00Z
modified: 2015-08-31T04:07:57Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732203_sequence/1

BBa_J61111
type: ComponentDefinition
persistent identity: BBa J61111
display id: BBa_J61111
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Ribosome Binding Site Family Member
creator: John Anderson
created: 2007-04-23T11:00:00Z
modified: 2015-08-31T02:03:00Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: false
mutable description: {{JCA_Arkin_RBSFamily}}
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J61111_sequence/1

pyrG casse
type: ComponentDefinition
persistent identity: BBa K678040
display id: BBa_K678040
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pyrG cassette, for selection of Aspergillus nidulans
creator: DTU-Denmark-2
created: 2011-09-03T11:00:00Z
modified: 2015-05-08T01:13:04Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _882_
m user id: 0
owner id: 8341
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: pyrG cassette, for selection of Aspergillus nidulans
mutable notes: Compatible with the Plug'n'Play assembly standard
mutable provenance: plasmid pDel2
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: pyrG-cassette
sequence annotation: Linker 5
sequence: https://synbiohub.org/public/igem/BBa_K678040_sequence/1

pyrG-cassette
type: SequenceAnnotation
persistent identity: annotation2127412
display id: annotation2127412
version: 1
top level: pyrG casse
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K678040/annotation2127412/range2127412/1

https://synbiohub.org/public/igem/BBa_K678040/annotation2127412/range2127412/1
type: Range
persistent identity: range2127412
display id: range2127412
version: 1
direction: direction/forward
top level: pyrG casse
start: 9
end: 1456
orientation: inline

Linker 5
type: SequenceAnnotation
persistent identity: annotation2127411
display id: annotation2127411
version: 1
top level: pyrG casse
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K678040/annotation2127411/range2127411/1

https://synbiohub.org/public/igem/BBa_K678040/annotation2127411/range2127411/1
type: Range
persistent identity: range2127411
display id: range2127411
version: 1
direction: direction/forward
top level: pyrG casse
start: 1
end: 8
orientation: inline

BBa_J48104
type: ComponentDefinition
persistent identity: BBa J48104
display id: BBa_J48104
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: NikR promoter, a protein of the ribbon helix-helix family of trancription factors that repress expre
creator: Laina Magaya
created: 2006-10-30T12:00:00Z
modified: 2015-08-31T04:08:51Z
discontinued: false
dominant: false
experience: experience/None
group u list: _73_
m user id: 0
owner id: 969
owning group id: 73
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Nickel responsive transcriptional regulator
mutable notes: Cut with Pst I and Xba I
mutable provenance: Escherichia coli UTI89
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J48104_sequence/1

BBa_K1486026
type: ComponentDefinition
persistent identity: BBa K1486026
display id: BBa_K1486026
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: sfGFP + Kanamycin resistance for yeast
creator: EPFL iGem team 2014
created: 2014-09-25T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: To edit
mutable notes: To edit
mutable provenance: To edit
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000324
role: partType/Tag
sequence annotation: BBa_K1486024
sequence annotation: Adh1 terminator
sequence annotation: BBa_K1486023
sequence annotation: 8 a.a linker
sequence annotation: Pbs2 flanking
sequence: https://synbiohub.org/public/igem/BBa_K1486026_sequence/1

BBa_K1486024
type: SequenceAnnotation
persistent identity: annotation2417051
display id: annotation2417051
version: 1
top level: BBa_K1486026
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1486026/annotation2417051/range2417051/1

https://synbiohub.org/public/igem/BBa_K1486026/annotation2417051/range2417051/1
type: Range
persistent identity: range2417051
display id: range2417051
version: 1
direction: direction/forward
top level: BBa_K1486026
start: 1073
end: 2430
orientation: inline

Adh1 terminator
type: SequenceAnnotation
persistent identity: annotation2417050
display id: annotation2417050
version: 1
top level: BBa_K1486026
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1486026/annotation2417050/range2417050/1

https://synbiohub.org/public/igem/BBa_K1486026/annotation2417050/range2417050/1
type: Range
persistent identity: range2417050
display id: range2417050
version: 1
direction: direction/forward
top level: BBa_K1486026
start: 811
end: 999
orientation: inline

BBa_K1486023
type: SequenceAnnotation
persistent identity: annotation2417049
display id: annotation2417049
version: 1
top level: BBa_K1486026
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1486026/annotation2417049/range2417049/1

https://synbiohub.org/public/igem/BBa_K1486026/annotation2417049/range2417049/1
type: Range
persistent identity: range2417049
display id: range2417049
version: 1
direction: direction/forward
top level: BBa_K1486026
start: 75
end: 791
orientation: inline

8 a.a linker
type: SequenceAnnotation
persistent identity: annotation2417048
display id: annotation2417048
version: 1
top level: BBa_K1486026
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1486026/annotation2417048/range2417048/1

https://synbiohub.org/public/igem/BBa_K1486026/annotation2417048/range2417048/1
type: Range
persistent identity: range2417048
display id: range2417048
version: 1
direction: direction/forward
top level: BBa_K1486026
start: 51
end: 74
orientation: inline

Pbs2 flanking
type: SequenceAnnotation
persistent identity: annotation2417047
display id: annotation2417047
version: 1
top level: BBa_K1486026
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1486026/annotation2417047/range2417047/1

https://synbiohub.org/public/igem/BBa_K1486026/annotation2417047/range2417047/1
type: Range
persistent identity: range2417047
display id: range2417047
version: 1
direction: direction/forward
top level: BBa_K1486026
start: 1
end: 50
orientation: inline

Pbs2 flanking
type: SequenceAnnotation
persistent identity: annotation2417052
display id: annotation2417052
version: 1
top level: BBa_K1486026
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1486026/annotation2417052/range2417052/1

https://synbiohub.org/public/igem/BBa_K1486026/annotation2417052/range2417052/1
type: Range
persistent identity: range2417052
display id: range2417052
version: 1
direction: direction/forward
top level: BBa_K1486026
start: 2485
end: 2535
orientation: inline

BBa_K678016
type: ComponentDefinition
persistent identity: BBa K678016
display id: BBa_K678016
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: eCFP-TS
creator: DTU-Denmark-2
created: 2011-09-12T11:00:00Z
modified: 2015-05-08T01:13:04Z
discontinued: false
dominant: false
experience: experience/None
group u list: _882_
m user id: 0
owner id: 10556
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part was designed for the Plug'n'Play with DNA assembly standard (RFC 80), therefore this part contains tails at the 5' and 3' ends as shown on the figure below.
mutable notes: No
mutable provenance: The Danish Cancer Society
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: Linker 3
sequence annotation: eCFP-TS
sequence annotation: Kozak
sequence: https://synbiohub.org/public/igem/BBa_K678016_sequence/1

Linker 3
type: SequenceAnnotation
persistent identity: annotation2127653
display id: annotation2127653
version: 1
top level: BBa_K678016
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K678016/annotation2127653/range2127653/1

https://synbiohub.org/public/igem/BBa_K678016/annotation2127653/range2127653/1
type: Range
persistent identity: range2127653
display id: range2127653
version: 1
direction: direction/forward
top level: BBa_K678016
start: 1
end: 9
orientation: inline

eCFP-TS
type: SequenceAnnotation
persistent identity: annotation2127659
display id: annotation2127659
version: 1
top level: BBa_K678016
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K678016/annotation2127659/range2127659/1

https://synbiohub.org/public/igem/BBa_K678016/annotation2127659/range2127659/1
type: Range
persistent identity: range2127659
display id: range2127659
version: 1
direction: direction/forward
top level: BBa_K678016
start: 17
end: 814
orientation: inline

Kozak
type: SequenceAnnotation
persistent identity: annotation2127794
display id: annotation2127794
version: 1
top level: BBa_K678016
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K678016/annotation2127794/range2127794/1

https://synbiohub.org/public/igem/BBa_K678016/annotation2127794/range2127794/1
type: Range
persistent identity: range2127794
display id: range2127794
version: 1
direction: direction/forward
top level: BBa_K678016
start: 10
end: 20
orientation: inline

p68
type: ComponentDefinition
persistent identity: BBa K678070
display id: BBa_K678070
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: p68
creator: DTU-Denmark-2
created: 2011-09-12T11:00:00Z
modified: 2015-05-08T01:13:05Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _882_
m user id: 0
owner id: 8341
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: To be announced
mutable notes: To be announced
mutable provenance: To be announced
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000155
role: partType/Plasmid
sequence annotation: lacZ
sequence annotation: USER cassette (AsiSI/Nb.BtsI)
sequence annotation: argB marker cassette
sequence annotation: TtrpC
sequence annotation: IS-up
sequence annotation: ampR
sequence annotation: IS-down
sequence: https://synbiohub.org/public/igem/BBa_K678070_sequence/1

lacZ
type: SequenceAnnotation
persistent identity: annotation2135108
display id: annotation2135108
version: 1
top level: p68
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K678070/annotation2135108/range2135108/1

https://synbiohub.org/public/igem/BBa_K678070/annotation2135108/range2135108/1
type: Range
persistent identity: range2135108
display id: range2135108
version: 1
direction: direction/forward
top level: p68
start: 3319
end: 6392
orientation: inline

USER cassette (AsiSI/Nb.BtsI)
type: SequenceAnnotation
persistent identity: annotation2135107
display id: annotation2135107
version: 1
top level: p68
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K678070/annotation2135107/range2135107/1

https://synbiohub.org/public/igem/BBa_K678070/annotation2135107/range2135107/1
type: Range
persistent identity: range2135107
display id: range2135107
version: 1
direction: direction/forward
top level: p68
start: 3285
end: 3318
orientation: inline

argB marker cassette
type: SequenceAnnotation
persistent identity: annotation2135110
display id: annotation2135110
version: 1
top level: p68
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K678070/annotation2135110/range2135110/1

https://synbiohub.org/public/igem/BBa_K678070/annotation2135110/range2135110/1
type: Range
persistent identity: range2135110
display id: range2135110
version: 1
direction: direction/forward
top level: p68
start: 7171
end: 9327
orientation: inline

TtrpC
type: SequenceAnnotation
persistent identity: annotation2135109
display id: annotation2135109
version: 1
top level: p68
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_K678070/annotation2135109/range2135109/1

https://synbiohub.org/public/igem/BBa_K678070/annotation2135109/range2135109/1
type: Range
persistent identity: range2135109
display id: range2135109
version: 1
direction: direction/forward
top level: p68
start: 6396
end: 7157
orientation: inline

IS-up
type: SequenceAnnotation
persistent identity: annotation2135106
display id: annotation2135106
version: 1
top level: p68
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K678070/annotation2135106/range2135106/1

https://synbiohub.org/public/igem/BBa_K678070/annotation2135106/range2135106/1
type: Range
persistent identity: range2135106
display id: range2135106
version: 1
direction: direction/forward
top level: p68
start: 1318
end: 3266
orientation: inline

ampR
type: SequenceAnnotation
persistent identity: annotation2135105
display id: annotation2135105
version: 1
top level: p68
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K678070/annotation2135105/range2135105/1

https://synbiohub.org/public/igem/BBa_K678070/annotation2135105/range2135105/1
type: Range
persistent identity: range2135105
display id: range2135105
version: 1
direction: direction/forward
top level: p68
start: 1
end: 859
orientation: inline

IS-down
type: SequenceAnnotation
persistent identity: annotation2135111
display id: annotation2135111
version: 1
top level: p68
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K678070/annotation2135111/range2135111/1

https://synbiohub.org/public/igem/BBa_K678070/annotation2135111/range2135111/1
type: Range
persistent identity: range2135111
display id: range2135111
version: 1
direction: direction/forward
top level: p68
start: 9342
end: 11267
orientation: inline

BBa_K116001
type: ComponentDefinition
persistent identity: BBa K116001
display id: BBa_K116001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: nhaA promoter, that can be regulated by pH and nhaR protein.
creator: Chih-Hsien Yang
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:09:32Z
discontinued: false
dominant: true
group u list: _210_
m user id: 0
owner id: 592
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: It's E.coli K12 sodium/proton anti-transport promoter. It can be a pH sensor. We test pH senses in different pH value and Sodium concentration.
mutable notes: It should contain NhaR binding site.
mutable provenance: E.coli K12 MG1655 genomic DNA
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K116001_sequence/1

HSP-LacI
type: ComponentDefinition
persistent identity: BBa K338002
display id: BBa_K338002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: K338001+R0011: Heat Shock Promoter + LacI Regulated Promoter
creator: 2010 Caltech iGEM Team
created: 2010-10-23T11:00:00Z
modified: 2015-05-08T01:12:07Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _454_
m user id: 0
owner id: 6189
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: Fused heat shock promoter with lacI regulated promoter. It possesses the characteristic of both heat shock promoter and LacI.
mutable notes: It was designed to address the issue of a leaky promoter in front of a bacterial gene in an effort to create a genetic switch just for growth/cloning
mutable provenance: containing K338001 and R0011
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
component: BBa_R0011
component: BBa_B0034
component: BBa_K338001
sequence annotation: BBa_R0011
sequence annotation: BBa_K338001
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K338002_sequence/1

BBa_R0011
type: Component
persistent identity: component2104036
display id: component2104036
version: 1
top level: HSP-LacI
access: public
definition: lacI+pL

BBa_B0034
type: Component
persistent identity: component2104042
display id: component2104042
version: 1
top level: HSP-LacI
access: public
definition: BBa_B0034

BBa_K338001
type: Component
persistent identity: component2104035
display id: component2104035
version: 1
top level: HSP-LacI
access: public
definition: HSP

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation2104036
display id: annotation2104036
version: 1
top level: HSP-LacI
location: https://synbiohub.org/public/igem/BBa_K338002/annotation2104036/range2104036/1
component: BBa_R0011

https://synbiohub.org/public/igem/BBa_K338002/annotation2104036/range2104036/1
type: Range
persistent identity: range2104036
display id: range2104036
version: 1
direction: direction/forward
top level: HSP-LacI
start: 107
end: 160
orientation: inline

BBa_K338001
type: SequenceAnnotation
persistent identity: annotation2104035
display id: annotation2104035
version: 1
top level: HSP-LacI
location: https://synbiohub.org/public/igem/BBa_K338002/annotation2104035/range2104035/1
component: BBa_K338001

https://synbiohub.org/public/igem/BBa_K338002/annotation2104035/range2104035/1
type: Range
persistent identity: range2104035
display id: range2104035
version: 1
direction: direction/reverse
top level: HSP-LacI
start: 1
end: 98
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2104042
display id: annotation2104042
version: 1
top level: HSP-LacI
location: https://synbiohub.org/public/igem/BBa_K338002/annotation2104042/range2104042/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K338002/annotation2104042/range2104042/1
type: Range
persistent identity: range2104042
display id: range2104042
version: 1
direction: direction/reverse
top level: HSP-LacI
start: 170
end: 181
orientation: inline

Pnot(O77)
type: ComponentDefinition
persistent identity: BBa I732207
display id: BBa_I732207
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: NOT Gate Promoter Family Member (D001O77)
creator: Zhan Jian
created: 2007-10-13T11:00:00Z
modified: 2015-08-31T04:07:57Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732207_sequence/1

RhlR+C4
type: ComponentDefinition
persistent identity: BBa R0071
display id: BBa_R0071
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter (RhlR & C4-HSL regulated)
creator: Ronny Krashinsky
created: 2004-01-24T12:00:00Z
modified: 2015-05-08T01:14:15Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Promoter activated by RhlR in concert with C4-HSL. C4-HSL is produced by RhlI, and acts as a quorum-sensing autoinducer. This is the natural sequence taken from Pseudomonas aeruginosa. Crosstalk: this promoter is also activated at a low level by LasR with its associated HSL.
mutable notes: The -10 and -35 sites are labeled as identified in [Pearson97]. The part begins with the RhlR binding site, and (rather arbitrarily) ends with the first transcribed base (+1).
mutable provenance: Pseudomonas aeruginosa rhlAB promoter
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: RhlR
sequence annotation: -10
sequence annotation: start
sequence annotation: -35
sequence: https://synbiohub.org/public/igem/BBa_R0071_sequence/1

RhlR
type: SequenceAnnotation
persistent identity: annotation297102
display id: annotation297102
version: 1
top level: RhlR+C4
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0071/annotation297102/range297102/1

https://synbiohub.org/public/igem/BBa_R0071/annotation297102/range297102/1
type: Range
persistent identity: range297102
display id: range297102
version: 1
direction: direction/forward
top level: RhlR+C4
start: 1
end: 20
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation297104
display id: annotation297104
version: 1
top level: RhlR+C4
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0071/annotation297104/range297104/1

https://synbiohub.org/public/igem/BBa_R0071/annotation297104/range297104/1
type: Range
persistent identity: range297104
display id: range297104
version: 1
direction: direction/forward
top level: RhlR+C4
start: 42
end: 47
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation297105
display id: annotation297105
version: 1
top level: RhlR+C4
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_R0071/annotation297105/range297105/1

https://synbiohub.org/public/igem/BBa_R0071/annotation297105/range297105/1
type: Range
persistent identity: range297105
display id: range297105
version: 1
direction: direction/forward
top level: RhlR+C4
start: 53
end: 53
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation297103
display id: annotation297103
version: 1
top level: RhlR+C4
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0071/annotation297103/range297103/1

https://synbiohub.org/public/igem/BBa_R0071/annotation297103/range297103/1
type: Range
persistent identity: range297103
display id: range297103
version: 1
direction: direction/forward
top level: RhlR+C4
start: 19
end: 24
orientation: inline

BBa_J61135
type: ComponentDefinition
persistent identity: BBa J61135
display id: BBa_J61135
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Ribosome Binding Site Family Member
creator: John Anderson
created: 2007-04-24T11:00:00Z
modified: 2015-08-31T01:59:49Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: {{JCA_Arkin_RBSFamily}}
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J61135_sequence/1

PenI
type: ComponentDefinition
persistent identity: BBa R0074
display id: BBa_R0074
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter (PenI regulated)
creator: crackdots
created: 2004-01-27T12:00:00Z
modified: 2015-05-08T01:14:15Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: PenP operator from Bacillus Lichenformis. Two operator sites are negatively regulated by PenI (C0074).
mutable notes: extends slightly past -50
mutable provenance: bacillus licheniformis
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -35
sequence annotation: -10
sequence annotation: dimer left half
sequence annotation: PenI
sequence annotation: dimer right half
sequence: https://synbiohub.org/public/igem/BBa_R0074_sequence/1

-35
type: SequenceAnnotation
persistent identity: annotation319211
display id: annotation319211
version: 1
top level: PenI
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0074/annotation319211/range319211/1

https://synbiohub.org/public/igem/BBa_R0074/annotation319211/range319211/1
type: Range
persistent identity: range319211
display id: range319211
version: 1
direction: direction/forward
top level: PenI
start: 23
end: 28
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation319212
display id: annotation319212
version: 1
top level: PenI
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0074/annotation319212/range319212/1

https://synbiohub.org/public/igem/BBa_R0074/annotation319212/range319212/1
type: Range
persistent identity: range319212
display id: range319212
version: 1
direction: direction/forward
top level: PenI
start: 46
end: 51
orientation: inline

dimer left half
type: SequenceAnnotation
persistent identity: annotation319788
display id: annotation319788
version: 1
top level: PenI
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0074/annotation319788/range319788/1

https://synbiohub.org/public/igem/BBa_R0074/annotation319788/range319788/1
type: Range
persistent identity: range319788
display id: range319788
version: 1
direction: direction/forward
top level: PenI
start: 31
end: 53
orientation: inline

PenI
type: SequenceAnnotation
persistent identity: annotation302713
display id: annotation302713
version: 1
top level: PenI
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_R0074/annotation302713/range302713/1

https://synbiohub.org/public/igem/BBa_R0074/annotation302713/range302713/1
type: Range
persistent identity: range302713
display id: range302713
version: 1
direction: direction/forward
top level: PenI
start: 1
end: 77
orientation: inline

dimer right half
type: SequenceAnnotation
persistent identity: annotation319789
display id: annotation319789
version: 1
top level: PenI
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0074/annotation319789/range319789/1

https://synbiohub.org/public/igem/BBa_R0074/annotation319789/range319789/1
type: Range
persistent identity: range319789
display id: range319789
version: 1
direction: direction/forward
top level: PenI
start: 55
end: 76
orientation: inline

BBa_K136004
type: ComponentDefinition
persistent identity: BBa K136004
display id: BBa_K136004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter of flhDC gene from E. coli flagella
creator: Cyprien Maisonnier
created: 2008-08-11T11:00:00Z
modified: 2015-05-08T01:10:02Z
discontinued: true
dominant: false
experience: experience/None
group u list: _211_
m user id: 0
owner id: 3012
owning group id: 9
sample status: Discontinued
status: status/Deleted
bookmark: false
mutable description: ''flhDC'' is the only class 1 gene that is part of the construction of the flagella. It is the master regulator of the biosynthesis of the flagella ( ''Ordering Genes in a Flagella Pathway by Analysis of Expression Kinetics from Living Bacteria'' - S. Kalir, J. McClure, K. Pabbaraju, C. Southward, M. Ronen, S. Leibler, M. G. Surette, U. Alon ). The promoter of ''flhDC'' is regulated by many transcription factors, for instance, OmpR* and EnvZ* are two repressors of this gene. '''Work in progress :''' Characterization of the repression by OmpR* and EnvZ*
mutable notes: The PCR on colony to amplify the promoter of ''flhDC'' did not work. We amplified both the promoter and the gene and then we did a specific amplification of the promoter from the full operon. We had a PCR amplicon of the right length this time. The primers used to amplify the promoter and the gene are : * Forward : GTTTCTTCGAATTCGCGGCCGCTTCTAGAGTTGTATGTGCGTGTAGTGACGAGTACAG * Reverse : GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTAAACAGCCTGTACTCTCTGTTCATCC Then to amplify only the promoter we used : * Forward : GTTTCTTCGAATTCGCGGCCGCTTCTAGAGTTGTATGTGCGTGTAGTGACGAGTACAG * Reverse : GTTTCTTCCTGCAGCGGCCGCTACTAGTATCCCACCCAGAATAACCAACTTTAT
mutable provenance: This part comes from the genome of ''E.coli'' K12 strain MG1655.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: OmpR binding site
sequence annotation: Transcription start base
sequence: https://synbiohub.org/public/igem/BBa_K136004_sequence/1

OmpR binding site
type: SequenceAnnotation
persistent identity: annotation1971578
display id: annotation1971578
version: 1
top level: BBa_K136004
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K136004/annotation1971578/range1971578/1

https://synbiohub.org/public/igem/BBa_K136004/annotation1971578/range1971578/1
type: Range
persistent identity: range1971578
display id: range1971578
version: 1
direction: direction/forward
top level: BBa_K136004
start: 43
end: 63
orientation: inline

Transcription start base
type: SequenceAnnotation
persistent identity: annotation1971577
display id: annotation1971577
version: 1
top level: BBa_K136004
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K136004/annotation1971577/range1971577/1

https://synbiohub.org/public/igem/BBa_K136004/annotation1971577/range1971577/1
type: Range
persistent identity: range1971577
display id: range1971577
version: 1
direction: direction/forward
top level: BBa_K136004
start: 36
end: 36
orientation: inline

BBa_I756016
type: ComponentDefinition
persistent identity: BBa I756016
display id: BBa_I756016
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CMV-tet promoter
creator: Jack
created: 2007-08-15T11:00:00Z
modified: 2015-08-31T04:08:06Z
discontinued: false
dominant: false
group u list: _108_
m user id: 0
owner id: 2067
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: CMV promoter with tet operator
mutable notes: CMV promoter with tet operator
mutable provenance: CMV promoter with tet operator
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: tet operator
sequence: https://synbiohub.org/public/igem/BBa_I756016_sequence/1

tet operator
type: SequenceAnnotation
persistent identity: annotation1941581
display id: annotation1941581
version: 1
top level: BBa_I756016
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I756016/annotation1941581/range1941581/1

https://synbiohub.org/public/igem/BBa_I756016/annotation1941581/range1941581/1
type: Range
persistent identity: range1941581
display id: range1941581
version: 1
direction: direction/forward
top level: BBa_I756016
start: 525
end: 578
orientation: inline

BBa_J23113
type: ComponentDefinition
persistent identity: BBa J23113
display id: BBa_J23113
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: constitutive promoter family member
creator: John Anderson
created: 2006-08-16T11:00:00Z
modified: 2015-08-31T04:08:40Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: Later
mutable notes: N/A
mutable provenance: Later
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J23113_sequence/1

BBa_K1165004
type: ComponentDefinition
persistent identity: BBa K1165004
display id: BBa_K1165004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PenI-cleaving Ribozyme 1 of XOR Gate 2
creator: iGEM Concordia 2013
created: 2013-09-26T11:00:00Z
modified: 2015-05-08T01:09:33Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1477_
m user id: 0
owner id: 18237
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: -
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000834
role: partType/RNA
sequence annotation: Target Binding Arms
sequence annotation: Target-Binding Arms
sequence annotation: Synthetic Extended Arms
sequence annotation: Catalytic Core
sequence annotation: TAA Bump
sequence: https://synbiohub.org/public/igem/BBa_K1165004_sequence/1

Target Binding Arms
type: SequenceAnnotation
persistent identity: annotation2365277
display id: annotation2365277
version: 1
top level: BBa_K1165004
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1165004/annotation2365277/range2365277/1

https://synbiohub.org/public/igem/BBa_K1165004/annotation2365277/range2365277/1
type: Range
persistent identity: range2365277
display id: range2365277
version: 1
direction: direction/forward
top level: BBa_K1165004
start: 39
end: 43
orientation: inline

Target-Binding Arms
type: SequenceAnnotation
persistent identity: annotation2365272
display id: annotation2365272
version: 1
top level: BBa_K1165004
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1165004/annotation2365272/range2365272/1

https://synbiohub.org/public/igem/BBa_K1165004/annotation2365272/range2365272/1
type: Range
persistent identity: range2365272
display id: range2365272
version: 1
direction: direction/forward
top level: BBa_K1165004
start: 33
end: 35
orientation: inline

Target-Binding Arms
type: SequenceAnnotation
persistent identity: annotation2365279
display id: annotation2365279
version: 1
top level: BBa_K1165004
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1165004/annotation2365279/range2365279/1

https://synbiohub.org/public/igem/BBa_K1165004/annotation2365279/range2365279/1
type: Range
persistent identity: range2365279
display id: range2365279
version: 1
direction: direction/forward
top level: BBa_K1165004
start: 70
end: 81
orientation: inline

Synthetic Extended Arms
type: SequenceAnnotation
persistent identity: annotation2365280
display id: annotation2365280
version: 1
top level: BBa_K1165004
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1165004/annotation2365280/range2365280/1

https://synbiohub.org/public/igem/BBa_K1165004/annotation2365280/range2365280/1
type: Range
persistent identity: range2365280
display id: range2365280
version: 1
direction: direction/forward
top level: BBa_K1165004
start: 82
end: 111
orientation: inline

Catalytic Core
type: SequenceAnnotation
persistent identity: annotation2365278
display id: annotation2365278
version: 1
top level: BBa_K1165004
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1165004/annotation2365278/range2365278/1

https://synbiohub.org/public/igem/BBa_K1165004/annotation2365278/range2365278/1
type: Range
persistent identity: range2365278
display id: range2365278
version: 1
direction: direction/forward
top level: BBa_K1165004
start: 44
end: 69
orientation: inline

TAA Bump
type: SequenceAnnotation
persistent identity: annotation2365273
display id: annotation2365273
version: 1
top level: BBa_K1165004
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1165004/annotation2365273/range2365273/1

https://synbiohub.org/public/igem/BBa_K1165004/annotation2365273/range2365273/1
type: Range
persistent identity: range2365273
display id: range2365273
version: 1
direction: direction/forward
top level: BBa_K1165004
start: 36
end: 38
orientation: inline

Synthetic Extended Arms
type: SequenceAnnotation
persistent identity: annotation2365271
display id: annotation2365271
version: 1
top level: BBa_K1165004
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1165004/annotation2365271/range2365271/1

https://synbiohub.org/public/igem/BBa_K1165004/annotation2365271/range2365271/1
type: Range
persistent identity: range2365271
display id: range2365271
version: 1
direction: direction/forward
top level: BBa_K1165004
start: 1
end: 30
orientation: inline

BBa_M13510
type: ComponentDefinition
persistent identity: BBa M13510
display id: BBa_M13510
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: M13KO7 gene X RBS
creator: Natalie Kuldell
created: 2006-12-14T12:00:00Z
modified: 2015-05-08T01:13:56Z
discontinued: false
dominant: false
experience: experience/None
group u list: _45_
m user id: 0
owner id: 314
owning group id: 1
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This RBS is part of the bacteriophage M13 genome, initiating translation of the gene X message (BBa_M13010). The part aligns with base pairs 480-495 in M13K07 genome. It was identified as the RBS for gene II message by Wezenbeek et al in Gene (1980) 11: 129-148
mutable notes: none
mutable provenance: New England Biolabs M13K07. Sequenced in 2002 by L. Panganaban
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_M13510_sequence/1

BBa_J34801
type: ComponentDefinition
persistent identity: BBa J34801
display id: BBa_J34801
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: ribosome binding site
creator: Alexandra Choutko
created: 2006-10-25T11:00:00Z
modified: 2015-08-31T04:08:46Z
discontinued: false
dominant: false
experience: experience/None
group u list: _62_
m user id: 0
owner id: 1085
owning group id: 62
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: a
mutable notes: a
mutable provenance: a
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J34801_sequence/1

BBa_K1585104
type: ComponentDefinition
persistent identity: BBa K1585104
display id: BBa_K1585104
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Anderson Promoter with lacI binding site
creator: Tobias Schwanemann
created: 2015-08-31T11:00:00Z
modified: 2015-09-01T03:26:03Z
discontinued: false
dominant: false
experience: experience/Fails
group u list: _2002_
m user id: 24474
owner id: 24474
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Promoters of the Anderson Promoter Collection with lacI binding site derived from [http://parts.igem.org/Part:BBa_R0010 R_0010] to make them [http://openwetware.org/wiki/IPTG IPTG] iducible.
mutable notes: We choosed lacI-binding-site of part BBa_R0010 because we assumed older parts would be more reliable. The part is shipped in backbone [http://parts.igem.org/Part:BBa_J61002 J61002] because the functionality is directly visible by adding IPTG. RFP ([http://parts.igem.org/Part:BBa_E1010 E1010]) should get expressed then.
mutable provenance: Assambly of [http://parts.igem.org/Part:BBa_J23104 J23104] and lacI-binding-site of [http://parts.igem.org/Part:BBa_R0010 R_0010] The "lacI binding site" of Part [http://parts.igem.org/Part:BBa_R0010 R_0010] differs from lacO from Part [http://parts.igem.org/Part:BBa_J33207 BBa_J33207].
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
component: BBa_K1585999
component: BBa_J23104
sequence annotation: BBa_K1585999
sequence annotation: BBa_J23104
sequence: https://synbiohub.org/public/igem/BBa_K1585104_sequence/1

BBa_K1585999
type: Component
persistent identity: component2441422
display id: component2441422
version: 1
top level: BBa_K1585104
access: public
definition: https://synbiohub.org/public/igem/BBa_K1585999/1

BBa_J23104
type: Component
persistent identity: component2441420
display id: component2441420
version: 1
top level: BBa_K1585104
access: public
definition: BBa_J23104

BBa_K1585999
type: SequenceAnnotation
persistent identity: annotation2441422
display id: annotation2441422
version: 1
top level: BBa_K1585104
location: https://synbiohub.org/public/igem/BBa_K1585104/annotation2441422/range2441422/1
component: BBa_K1585999

https://synbiohub.org/public/igem/BBa_K1585104/annotation2441422/range2441422/1
type: Range
persistent identity: range2441422
display id: range2441422
version: 1
direction: direction/reverse
top level: BBa_K1585104
start: 44
end: 78
orientation: inline

BBa_J23104
type: SequenceAnnotation
persistent identity: annotation2441420
display id: annotation2441420
version: 1
top level: BBa_K1585104
location: https://synbiohub.org/public/igem/BBa_K1585104/annotation2441420/range2441420/1
component: BBa_J23104

https://synbiohub.org/public/igem/BBa_K1585104/annotation2441420/range2441420/1
type: Range
persistent identity: range2441420
display id: range2441420
version: 1
direction: direction/reverse
top level: BBa_K1585104
start: 1
end: 35
orientation: inline

BBa_J61139
type: ComponentDefinition
persistent identity: BBa J61139
display id: BBa_J61139
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Ribosome Binding Site Family Member
creator: John Anderson
created: 2007-04-24T11:00:00Z
modified: 2015-08-31T01:59:49Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: false
mutable description: {{JCA_Arkin_RBSFamily}}
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J61139_sequence/1

BBa_J61105
type: ComponentDefinition
persistent identity: BBa J61105
display id: BBa_J61105
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Ribosome Binding Site Family Member
creator: John Anderson
created: 2007-04-23T11:00:00Z
modified: 2015-08-31T02:03:00Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: false
mutable description: {{JCA_Arkin_RBSFamily}}
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J61105_sequence/1

OmpR
type: ComponentDefinition
persistent identity: BBa R0083
display id: BBa_R0083
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter (OmpR, positive)
creator: Stephen Lee, Roshan Kumar, Joe Levine, Ziyan Chu (Polkadorks, IAP 2004)
created: 2004-01-27T12:00:00Z
modified: 2015-05-08T01:14:15Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Positively regulated, OmpR-controlled promoter. This promoter is derived from the upstream region of ompC. Phosphorylated OmpR binds to the operator site and activates transcription. This is a truncated version of BBa_R0082.
mutable notes: The promoter deletes the C2 and C3 regions of BBa_R0082 (bases 37-74 in BBa_R0082) and inserts an 8-base linker before the -35 and -10 signal region. The efficacy of this promoter versus the full ompC upstream region (BBa_R0082) or the ompF upstream region (BBa_R0084) is currently unknown. The a at base 41 is mutated to t to avoid XbaI site. Speculation that this was a previous assembly site.
mutable provenance: NC_000193 E. coli K12
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: C1 OmpR
sequence annotation: A
sequence annotation: -35
sequence annotation: linker
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R0083_sequence/1

C1 OmpR
type: SequenceAnnotation
persistent identity: annotation301221
display id: annotation301221
version: 1
top level: OmpR
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0083/annotation301221/range301221/1

https://synbiohub.org/public/igem/BBa_R0083/annotation301221/range301221/1
type: Range
persistent identity: range301221
display id: range301221
version: 1
direction: direction/forward
top level: OmpR
start: 13
end: 30
orientation: inline

A
type: SequenceAnnotation
persistent identity: annotation306881
display id: annotation306881
version: 1
top level: OmpR
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_R0083/annotation306881/range306881/1

https://synbiohub.org/public/igem/BBa_R0083/annotation306881/range306881/1
type: Range
persistent identity: range306881
display id: range306881
version: 1
direction: direction/forward
top level: OmpR
start: 41
end: 41
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation301224
display id: annotation301224
version: 1
top level: OmpR
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0083/annotation301224/range301224/1

https://synbiohub.org/public/igem/BBa_R0083/annotation301224/range301224/1
type: Range
persistent identity: range301224
display id: range301224
version: 1
direction: direction/forward
top level: OmpR
start: 45
end: 50
orientation: inline

linker
type: SequenceAnnotation
persistent identity: annotation301223
display id: annotation301223
version: 1
top level: OmpR
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_R0083/annotation301223/range301223/1

https://synbiohub.org/public/igem/BBa_R0083/annotation301223/range301223/1
type: Range
persistent identity: range301223
display id: range301223
version: 1
direction: direction/forward
top level: OmpR
start: 37
end: 44
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation301226
display id: annotation301226
version: 1
top level: OmpR
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0083/annotation301226/range301226/1

https://synbiohub.org/public/igem/BBa_R0083/annotation301226/range301226/1
type: Range
persistent identity: range301226
display id: range301226
version: 1
direction: direction/forward
top level: OmpR
start: 68
end: 73
orientation: inline

BBa_J54130
type: ComponentDefinition
persistent identity: BBa J54130
display id: BBa_J54130
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: BetI_regulated promoter
creator: Yusaku Nakashima
created: 2006-10-20T11:00:00Z
modified: 2015-08-31T03:48:12Z
discontinued: false
dominant: false
group u list: _76_
m user id: 0
owner id: 762
owning group id: 76
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: BetI_regulated promoter
mutable notes: As BetI regulated
mutable provenance: DNA synthsis
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: BetI_regulated promoter
sequence annotation: BamHI
sequence: https://synbiohub.org/public/igem/BBa_J54130_sequence/1

BetI_regulated promoter
type: SequenceAnnotation
persistent identity: annotation1903812
display id: annotation1903812
version: 1
top level: BBa_J54130
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_J54130/annotation1903812/range1903812/1

https://synbiohub.org/public/igem/BBa_J54130/annotation1903812/range1903812/1
type: Range
persistent identity: range1903812
display id: range1903812
version: 1
direction: direction/forward
top level: BBa_J54130
start: 6
end: 43
orientation: inline

BamHI
type: SequenceAnnotation
persistent identity: annotation1903813
display id: annotation1903813
version: 1
top level: BBa_J54130
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_J54130/annotation1903813/range1903813/1

https://synbiohub.org/public/igem/BBa_J54130/annotation1903813/range1903813/1
type: Range
persistent identity: range1903813
display id: range1903813
version: 1
direction: direction/forward
top level: BBa_J54130
start: 1
end: 5
orientation: inline

BBa_J64998
type: ComponentDefinition
persistent identity: BBa J64998
display id: BBa_J64998
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: consensus -10 and rest from SP6
creator: Sai Duriseti
created: 2007-03-25T11:00:00Z
modified: 2015-05-08T01:08:18Z
discontinued: false
dominant: false
experience: experience/None
group u list: _98_
m user id: 0
owner id: 1428
owning group id: 98
sample status: Not in stock
status: status/Unavailable
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: Sigma 70 -10
sequence: https://synbiohub.org/public/igem/BBa_J64998_sequence/1

Sigma 70 -10
type: SequenceAnnotation
persistent identity: annotation1921745
display id: annotation1921745
version: 1
top level: BBa_J64998
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_J64998/annotation1921745/range1921745/1

https://synbiohub.org/public/igem/BBa_J64998/annotation1921745/range1921745/1
type: Range
persistent identity: range1921745
display id: range1921745
version: 1
direction: direction/forward
top level: BBa_J64998
start: 1
end: 19
orientation: inline

gmR
type: ComponentDefinition
persistent identity: BBa P1013
display id: BBa_P1013
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: gentamycin resistance cassette (aacC1 gene)
creator: Tom Knight
created: 2006-03-16T12:00:00Z
modified: 2015-05-08T01:14:12Z
discontinued: false
dominant: false
experience: experience/None
group u list: _41_48_1_
m user id: 0
owner id: 6
owning group id: 48
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: The aacC1 gene conveys resistance to gentamycin only. It is derived from a Tn21 series transposon (Wohlleben89) found on the R1033 plasmid (Pseudomonas aeruginosa). This part is created by PCR from the pJAT8 plasmid (Keastling lab) which in turn is derived from pJN105 (Newman and Fuqua).
mutable notes: pJAT8 plasmid edited with primers: GTT TCT TCG AAT TCG CGG CCG CTT CTA GAG ccttgtcgccttgcgtataa,aacC1-F GTT TCT TCC TGC AGC GGC CGC TAC TAG TA atctcggcttgaacgaattg,aacC1-R
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: aacC1 coding sequence
sequence annotation: -35 / -10 promoter sites
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_P1013_sequence/1

aacC1 coding sequence
type: SequenceAnnotation
persistent identity: annotation1826614
display id: annotation1826614
version: 1
top level: gmR
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_P1013/annotation1826614/range1826614/1

https://synbiohub.org/public/igem/BBa_P1013/annotation1826614/range1826614/1
type: Range
persistent identity: range1826614
display id: range1826614
version: 1
direction: direction/forward
top level: gmR
start: 288
end: 821
orientation: inline

-35 / -10 promoter sites
type: SequenceAnnotation
persistent identity: annotation1826613
display id: annotation1826613
version: 1
top level: gmR
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_P1013/annotation1826613/range1826613/1

https://synbiohub.org/public/igem/BBa_P1013/annotation1826613/range1826613/1
type: Range
persistent identity: range1826613
display id: range1826613
version: 1
direction: direction/forward
top level: gmR
start: 71
end: 99
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation1826615
display id: annotation1826615
version: 1
top level: gmR
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_P1013/annotation1826615/range1826615/1

https://synbiohub.org/public/igem/BBa_P1013/annotation1826615/range1826615/1
type: Range
persistent identity: range1826615
display id: range1826615
version: 1
direction: direction/forward
top level: gmR
start: 276
end: 279
orientation: inline

CheZ-GFP
type: ComponentDefinition
persistent identity: BBa K1709002
display id: BBa_K1709002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CheZ-GFP
creator: Astrid Deryckere
created: 2015-09-09T11:00:00Z
modified: 2015-09-18T09:28:45Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2129_
m user id: 25389
owner id: 25389
owning group id: 9
status: status/Planning
bookmark: true
mutable description: Upon recognition of a small molecule (e.g. nutrients or toxins), chemoreceptors transduce a signal through a set of Che proteins. This group of methylesterases and phosphatases regulates the rotation of the flagella. At the end of the chain of Che proteins is CheY. In phosphorylated state, CheY binds the flagella and causes the cells to tumble. The phosphatase CheZ dephosphorylates CheY, inducing dissociation of CheY from the flagella enabling the cells to swim. Cells lacking the CheZ protein are unable to swim and will tumble excessively and incessantly.
mutable notes: 1 bp in BBa_K629003 was adapted to create an original restriciton site in the plasmid. CheZ was fused to GFP (BBa_K082003) and its LVA tag was codon-optimized to avoid introducing artificial DNA repetitions.
mutable provenance: DNA sequences originate from the E. Coli genome and previous biobrick sequences.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: LVA-tag
sequence annotation: Ochre Stop Codon
sequence annotation: GFP Coding Region
sequence annotation: CheZ Coding Region
sequence annotation: ATG Start Codon
sequence: https://synbiohub.org/public/igem/BBa_K1709002_sequence/1

LVA-tag
type: SequenceAnnotation
persistent identity: annotation2448534
display id: annotation2448534
version: 1
top level: CheZ-GFP
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1709002/annotation2448534/range2448534/1

https://synbiohub.org/public/igem/BBa_K1709002/annotation2448534/range2448534/1
type: Range
persistent identity: range2448534
display id: range2448534
version: 1
direction: direction/forward
top level: CheZ-GFP
start: 1366
end: 1401
orientation: inline

Ochre Stop Codon
type: SequenceAnnotation
persistent identity: annotation2448531
display id: annotation2448531
version: 1
top level: CheZ-GFP
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1709002/annotation2448531/range2448531/1

https://synbiohub.org/public/igem/BBa_K1709002/annotation2448531/range2448531/1
type: Range
persistent identity: range2448531
display id: range2448531
version: 1
direction: direction/forward
top level: CheZ-GFP
start: 1399
end: 1401
orientation: inline

GFP Coding Region
type: SequenceAnnotation
persistent identity: annotation2448533
display id: annotation2448533
version: 1
top level: CheZ-GFP
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1709002/annotation2448533/range2448533/1

https://synbiohub.org/public/igem/BBa_K1709002/annotation2448533/range2448533/1
type: Range
persistent identity: range2448533
display id: range2448533
version: 1
direction: direction/forward
top level: CheZ-GFP
start: 646
end: 1365
orientation: inline

CheZ Coding Region
type: SequenceAnnotation
persistent identity: annotation2448532
display id: annotation2448532
version: 1
top level: CheZ-GFP
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1709002/annotation2448532/range2448532/1

https://synbiohub.org/public/igem/BBa_K1709002/annotation2448532/range2448532/1
type: Range
persistent identity: range2448532
display id: range2448532
version: 1
direction: direction/forward
top level: CheZ-GFP
start: 1
end: 642
orientation: inline

ATG Start Codon
type: SequenceAnnotation
persistent identity: annotation2448530
display id: annotation2448530
version: 1
top level: CheZ-GFP
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1709002/annotation2448530/range2448530/1

https://synbiohub.org/public/igem/BBa_K1709002/annotation2448530/range2448530/1
type: Range
persistent identity: range2448530
display id: range2448530
version: 1
direction: direction/forward
top level: CheZ-GFP
start: 1
end: 3
orientation: inline

BBa_K266008
type: ComponentDefinition
persistent identity: BBa K266008
display id: BBa_K266008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: J23100 + Lac inverter
creator: UNAM FCIENCIAS-CINVESTAV IRA
created: 2009-10-12T11:00:00Z
modified: 2015-05-08T01:11:43Z
discontinued: false
dominant: false
experience: experience/None
group u list: _363_
m user id: 0
owner id: 1955
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: The BBa_J23100 constitutive promoter with Lac systems inverter
mutable notes: None
mutable provenance: The biobricks BBa_J23100 and BBa_Q04121 where used to build this biobrick
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Inverter
component: BBa_Q04121
component: BBa_J23100
sequence annotation: BBa_J23100
sequence annotation: BBa_Q04121
sequence: https://synbiohub.org/public/igem/BBa_K266008_sequence/1

BBa_Q04121
type: Component
persistent identity: component2262964
display id: component2262964
version: 1
top level: BBa_K266008
access: public
definition: https://synbiohub.org/public/igem/BBa_Q04121/1

BBa_J23100
type: Component
persistent identity: component2262945
display id: component2262945
version: 1
top level: BBa_K266008
access: public
definition: BBa_J23100

BBa_J23100
type: SequenceAnnotation
persistent identity: annotation2262945
display id: annotation2262945
version: 1
top level: BBa_K266008
location: https://synbiohub.org/public/igem/BBa_K266008/annotation2262945/range2262945/1
component: BBa_J23100

https://synbiohub.org/public/igem/BBa_K266008/annotation2262945/range2262945/1
type: Range
persistent identity: range2262945
display id: range2262945
version: 1
direction: direction/reverse
top level: BBa_K266008
start: 1
end: 35
orientation: inline

BBa_Q04121
type: SequenceAnnotation
persistent identity: annotation2262964
display id: annotation2262964
version: 1
top level: BBa_K266008
location: https://synbiohub.org/public/igem/BBa_K266008/annotation2262964/range2262964/1
component: BBa_Q04121

https://synbiohub.org/public/igem/BBa_K266008/annotation2262964/range2262964/1
type: Range
persistent identity: range2262964
display id: range2262964
version: 1
direction: direction/reverse
top level: BBa_K266008
start: 44
end: 1414
orientation: inline

BBa_J05210
type: ComponentDefinition
persistent identity: BBa J05210
display id: BBa_J05210
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Modifed Prm+ Promoter
creator: Alexander Roth
created: 2005-09-05T11:00:00Z
modified: 2015-08-31T04:08:15Z
discontinued: false
dominant: false
group u list: _34_
m user id: 0
owner id: 412
owning group id: 34
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This is identical to BBa_I12007 exept that some base has been modifed to allow for additional ZFP repressors to bind.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -35
sequence annotation: OR2
sequence annotation: -10
sequence annotation: ZFP binding sites
sequence annotation: OR1
sequence: https://synbiohub.org/public/igem/BBa_J05210_sequence/1

-35
type: SequenceAnnotation
persistent identity: annotation1689959
display id: annotation1689959
version: 1
top level: BBa_J05210
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_J05210/annotation1689959/range1689959/1

https://synbiohub.org/public/igem/BBa_J05210/annotation1689959/range1689959/1
type: Range
persistent identity: range1689959
display id: range1689959
version: 1
direction: direction/forward
top level: BBa_J05210
start: 48
end: 53
orientation: inline

OR2
type: SequenceAnnotation
persistent identity: annotation1689958
display id: annotation1689958
version: 1
top level: BBa_J05210
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_J05210/annotation1689958/range1689958/1

https://synbiohub.org/public/igem/BBa_J05210/annotation1689958/range1689958/1
type: Range
persistent identity: range1689958
display id: range1689958
version: 1
direction: direction/forward
top level: BBa_J05210
start: 33
end: 49
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1689961
display id: annotation1689961
version: 1
top level: BBa_J05210
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_J05210/annotation1689961/range1689961/1

https://synbiohub.org/public/igem/BBa_J05210/annotation1689961/range1689961/1
type: Range
persistent identity: range1689961
display id: range1689961
version: 1
direction: direction/forward
top level: BBa_J05210
start: 71
end: 76
orientation: inline

ZFP binding sites
type: SequenceAnnotation
persistent identity: annotation1689960
display id: annotation1689960
version: 1
top level: BBa_J05210
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_J05210/annotation1689960/range1689960/1

https://synbiohub.org/public/igem/BBa_J05210/annotation1689960/range1689960/1
type: Range
persistent identity: range1689960
display id: range1689960
version: 1
direction: direction/forward
top level: BBa_J05210
start: 55
end: 66
orientation: inline

OR1
type: SequenceAnnotation
persistent identity: annotation1689957
display id: annotation1689957
version: 1
top level: BBa_J05210
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_J05210/annotation1689957/range1689957/1

https://synbiohub.org/public/igem/BBa_J05210/annotation1689957/range1689957/1
type: Range
persistent identity: range1689957
display id: range1689957
version: 1
direction: direction/forward
top level: BBa_J05210
start: 9
end: 25
orientation: inline

BBa_K091146
type: ComponentDefinition
persistent identity: BBa K091146
display id: BBa_K091146
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pLas/Lux Hybrid Promoter
creator: Erin Feeney
created: 2008-07-16T11:00:00Z
modified: 2015-05-08T01:08:38Z
discontinued: false
dominant: false
experience: experience/None
group u list: _191_
m user id: 0
owner id: 3067
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: K091146 is a modified version of the pLas' promoter (BBa_K091117) containing the addition of a luxR binding site between the -35 and -10 regions. This promoter is expected to be activated in the presence of PAI-1 and LasR and repressed in the presence of AI-1 and LuxR.
mutable notes: The Lux box was placed adjacent to the LasR binding site with the aim of minimizing cross-talk in the presence of both PAI-1 and AI-1; since the LasR site should have a higher affinity, we hope that the binding of PAI-1 will competitively exclude the binding of AI-1.
mutable provenance: The LuxR binding site is from BBa_R0062 and the pLas' promoter is from the region upstream of the LasI gene in Pseudomonas aeruginosa.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: LuxR
sequence annotation: -35
sequence annotation: -10
sequence annotation: LasR
sequence annotation: Transcriptional Start
sequence: https://synbiohub.org/public/igem/BBa_K091146_sequence/1

LuxR
type: SequenceAnnotation
persistent identity: annotation1967355
display id: annotation1967355
version: 1
top level: BBa_K091146
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K091146/annotation1967355/range1967355/1

https://synbiohub.org/public/igem/BBa_K091146/annotation1967355/range1967355/1
type: Range
persistent identity: range1967355
display id: range1967355
version: 1
direction: direction/forward
top level: BBa_K091146
start: 94
end: 113
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1967357
display id: annotation1967357
version: 1
top level: BBa_K091146
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K091146/annotation1967357/range1967357/1

https://synbiohub.org/public/igem/BBa_K091146/annotation1967357/range1967357/1
type: Range
persistent identity: range1967357
display id: range1967357
version: 1
direction: direction/forward
top level: BBa_K091146
start: 86
end: 91
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1967356
display id: annotation1967356
version: 1
top level: BBa_K091146
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K091146/annotation1967356/range1967356/1

https://synbiohub.org/public/igem/BBa_K091146/annotation1967356/range1967356/1
type: Range
persistent identity: range1967356
display id: range1967356
version: 1
direction: direction/forward
top level: BBa_K091146
start: 113
end: 118
orientation: inline

LasR
type: SequenceAnnotation
persistent identity: annotation1967354
display id: annotation1967354
version: 1
top level: BBa_K091146
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K091146/annotation1967354/range1967354/1

https://synbiohub.org/public/igem/BBa_K091146/annotation1967354/range1967354/1
type: Range
persistent identity: range1967354
display id: range1967354
version: 1
direction: direction/forward
top level: BBa_K091146
start: 75
end: 93
orientation: inline

Transcriptional Start
type: SequenceAnnotation
persistent identity: annotation1967358
display id: annotation1967358
version: 1
top level: BBa_K091146
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K091146/annotation1967358/range1967358/1

https://synbiohub.org/public/igem/BBa_K091146/annotation1967358/range1967358/1
type: Range
persistent identity: range1967358
display id: range1967358
version: 1
direction: direction/forward
top level: BBa_K091146
start: 125
end: 125
orientation: inline

BBa_K121016
type: ComponentDefinition
persistent identity: BBa K121016
display id: BBa_K121016
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Tokyo Standard prefix suffix RBS
creator: Shotaro Ayukawa
created: 2008-10-25T11:00:00Z
modified: 2015-05-08T01:09:41Z
discontinued: false
dominant: false
experience: experience/None
group u list: _220_
m user id: 0
owner id: 765
owning group id: 76
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Restriction sites of Sal I, Bam HI, Bgl II, and Mlu I in front of strong RBS
mutable notes: We designed new prefix(Sal I and Bam HI) and suffix(Bgl II and Mul I) for insertions of promoters and regulator binding sites in front of RBS.
mutable provenance: RBS is from BBa_0030
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: B0030
sequence annotation: Mlu I
sequence annotation: Bgl II
sequence annotation: Sal I
sequence annotation: Bam HI
sequence: https://synbiohub.org/public/igem/BBa_K121016_sequence/1

B0030
type: SequenceAnnotation
persistent identity: annotation1988216
display id: annotation1988216
version: 1
top level: BBa_K121016
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K121016/annotation1988216/range1988216/1

https://synbiohub.org/public/igem/BBa_K121016/annotation1988216/range1988216/1
type: Range
persistent identity: range1988216
display id: range1988216
version: 1
direction: direction/forward
top level: BBa_K121016
start: 50
end: 64
orientation: inline

Mlu I
type: SequenceAnnotation
persistent identity: annotation1988250
display id: annotation1988250
version: 1
top level: BBa_K121016
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K121016/annotation1988250/range1988250/1

https://synbiohub.org/public/igem/BBa_K121016/annotation1988250/range1988250/1
type: Range
persistent identity: range1988250
display id: range1988250
version: 1
direction: direction/forward
top level: BBa_K121016
start: 41
end: 46
orientation: inline

Bgl II
type: SequenceAnnotation
persistent identity: annotation1988248
display id: annotation1988248
version: 1
top level: BBa_K121016
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K121016/annotation1988248/range1988248/1

https://synbiohub.org/public/igem/BBa_K121016/annotation1988248/range1988248/1
type: Range
persistent identity: range1988248
display id: range1988248
version: 1
direction: direction/forward
top level: BBa_K121016
start: 26
end: 31
orientation: inline

Sal I
type: SequenceAnnotation
persistent identity: annotation1988246
display id: annotation1988246
version: 1
top level: BBa_K121016
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K121016/annotation1988246/range1988246/1

https://synbiohub.org/public/igem/BBa_K121016/annotation1988246/range1988246/1
type: Range
persistent identity: range1988246
display id: range1988246
version: 1
direction: direction/forward
top level: BBa_K121016
start: 1
end: 6
orientation: inline

Bam HI
type: SequenceAnnotation
persistent identity: annotation1988247
display id: annotation1988247
version: 1
top level: BBa_K121016
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K121016/annotation1988247/range1988247/1

https://synbiohub.org/public/igem/BBa_K121016/annotation1988247/range1988247/1
type: Range
persistent identity: range1988247
display id: range1988247
version: 1
direction: direction/forward
top level: BBa_K121016
start: 15
end: 20
orientation: inline

BBa_I721001
type: ComponentDefinition
persistent identity: BBa I721001
display id: BBa_I721001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Lead Promoter
creator: Jeffrey Hofmann
created: 2007-10-25T11:00:00Z
modified: 2015-08-31T04:07:53Z
discontinued: false
dominant: false
experience: experience/None
group u list: _113_
m user id: 0
owner id: 1531
owning group id: 58
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: Lead Promoter (Part 4) messed up EcoRI site
mutable notes: none
mutable provenance: ralstonia metallidurans
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I721001_sequence/1

BBa_J64024
type: ComponentDefinition
persistent identity: BBa J64024
display id: BBa_J64024
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: sipC RBS
creator: Dan Widmaier
created: 2008-01-16T12:00:00Z
modified: 2015-08-31T01:56:26Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _98_
m user id: 0
owner id: 2407
owning group id: 98
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This part is the natural RBS from the sipC protein in Salmonella Type III Secretion.
mutable notes: This part was not designed with biobrick standard assembly in mind. This RBS has not been explicitly measured for expression rate using fluorescence or B-Gal assay.
mutable provenance: Salmonella LT2 Genomic DNA
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: sipC RBS
sequence: https://synbiohub.org/public/igem/BBa_J64024_sequence/1

sipC RBS
type: SequenceAnnotation
persistent identity: annotation1959623
display id: annotation1959623
version: 1
top level: BBa_J64024
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_J64024/annotation1959623/range1959623/1

https://synbiohub.org/public/igem/BBa_J64024/annotation1959623/range1959623/1
type: Range
persistent identity: range1959623
display id: range1959623
version: 1
direction: direction/forward
top level: BBa_J64024
start: 1
end: 15
orientation: inline

BBa_I732451
type: ComponentDefinition
persistent identity: BBa I732451
display id: BBa_I732451
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Family Member (U073O27+D062NUL)
creator: Zhan Jian
created: 2007-10-16T11:00:00Z
modified: 2015-08-31T04:07:58Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732451_sequence/1

BBa_K1486010
type: ComponentDefinition
persistent identity: BBa K1486010
display id: BBa_K1486010
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CxpR & Split IFP1.4 [Nterm + Cterm]
creator: iGem EPFL 2014
created: 2014-08-26T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part has two fused proteins downstream of an arabinose-inducible promoter: CpxR-IFP[1] and CpxR-IFP[2]. This construct was made in all orientations: - CpxR-IFP[1] + CpxR-IFP[2] (BBa_K1286008) - IFP[1]-CpxR + CpxR-IFP[2] (BBa_K1486010) - CpxR-IFP[1] + IFP[2]-CpxR (BBa_K1486011)
mutable notes: All orientations were made, so all dimerizations could be tested (see main page for biobrick numbers). Flexible linkers were used for each of the fused proteins, and an RBS was put before each one of them.
mutable provenance: From Michnick Article. To Edit
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K1486004
component: BBa_I0500
component: BBa_K1486007
component: BBa_K1486006
component: BBa_B0034
component: BBa_K1486000
sequence annotation: BBa_K1486000
sequence annotation: BBa_K1486004
sequence annotation: BBa_B0034
sequence annotation: BBa_K1486006
sequence annotation: BBa_I0500
sequence annotation: BBa_K1486007
sequence: https://synbiohub.org/public/igem/BBa_K1486010_sequence/1

BBa_K1486004
type: Component
persistent identity: component2425002
display id: component2425002
version: 1
top level: BBa_K1486010
access: public
definition: BBa_K1486004

BBa_K1486004
type: Component
persistent identity: component2424991
display id: component2424991
version: 1
top level: BBa_K1486010
access: public
definition: BBa_K1486004

BBa_I0500
type: Component
persistent identity: component2424984
display id: component2424984
version: 1
top level: BBa_K1486010
access: public
definition: pBad/araC

BBa_K1486007
type: Component
persistent identity: component2425006
display id: component2425006
version: 1
top level: BBa_K1486010
access: public
definition: BBa_K1486007

BBa_K1486006
type: Component
persistent identity: component2424990
display id: component2424990
version: 1
top level: BBa_K1486010
access: public
definition: BBa_K1486006

BBa_B0034
type: Component
persistent identity: component2424997
display id: component2424997
version: 1
top level: BBa_K1486010
access: public
definition: BBa_B0034

BBa_K1486000
type: Component
persistent identity: component2425001
display id: component2425001
version: 1
top level: BBa_K1486010
access: public
definition: BBa_K1486000

BBa_K1486000
type: Component
persistent identity: component2424995
display id: component2424995
version: 1
top level: BBa_K1486010
access: public
definition: BBa_K1486000

BBa_B0034
type: Component
persistent identity: component2424986
display id: component2424986
version: 1
top level: BBa_K1486010
access: public
definition: BBa_B0034

BBa_K1486000
type: SequenceAnnotation
persistent identity: annotation2424995
display id: annotation2424995
version: 1
top level: BBa_K1486010
location: https://synbiohub.org/public/igem/BBa_K1486010/annotation2424995/range2424995/1
component: BBa_K1486000

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type: Range
persistent identity: range2424995
display id: range2424995
version: 1
direction: direction/reverse
top level: BBa_K1486010
start: 1658
end: 2359
orientation: inline

BBa_K1486004
type: SequenceAnnotation
persistent identity: annotation2424991
display id: annotation2424991
version: 1
top level: BBa_K1486010
location: https://synbiohub.org/public/igem/BBa_K1486010/annotation2424991/range2424991/1
component: BBa_K1486004

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type: Range
persistent identity: range2424991
display id: range2424991
version: 1
direction: direction/reverse
top level: BBa_K1486010
start: 1628
end: 1657
orientation: inline

BBa_K1486004
type: SequenceAnnotation
persistent identity: annotation2425002
display id: annotation2425002
version: 1
top level: BBa_K1486010
location: https://synbiohub.org/public/igem/BBa_K1486010/annotation2425002/range2425002/1
component: BBa_K1486004

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type: Range
persistent identity: range2425002
display id: range2425002
version: 1
direction: direction/reverse
top level: BBa_K1486010
start: 3074
end: 3103
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2424997
display id: annotation2424997
version: 1
top level: BBa_K1486010
location: https://synbiohub.org/public/igem/BBa_K1486010/annotation2424997/range2424997/1
component: BBa_B0034

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type: Range
persistent identity: range2424997
display id: range2424997
version: 1
direction: direction/reverse
top level: BBa_K1486010
start: 2360
end: 2371
orientation: inline

BBa_K1486000
type: SequenceAnnotation
persistent identity: annotation2425001
display id: annotation2425001
version: 1
top level: BBa_K1486010
location: https://synbiohub.org/public/igem/BBa_K1486010/annotation2425001/range2425001/1
component: BBa_K1486000

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type: Range
persistent identity: range2425001
display id: range2425001
version: 1
direction: direction/reverse
top level: BBa_K1486010
start: 2372
end: 3073
orientation: inline

BBa_K1486006
type: SequenceAnnotation
persistent identity: annotation2424990
display id: annotation2424990
version: 1
top level: BBa_K1486010
location: https://synbiohub.org/public/igem/BBa_K1486010/annotation2424990/range2424990/1
component: BBa_K1486006

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type: Range
persistent identity: range2424990
display id: range2424990
version: 1
direction: direction/reverse
top level: BBa_K1486010
start: 1223
end: 1627
orientation: inline

BBa_I0500
type: SequenceAnnotation
persistent identity: annotation2424984
display id: annotation2424984
version: 1
top level: BBa_K1486010
location: https://synbiohub.org/public/igem/BBa_K1486010/annotation2424984/range2424984/1
component: BBa_I0500

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type: Range
persistent identity: range2424984
display id: range2424984
version: 1
direction: direction/reverse
top level: BBa_K1486010
start: 1
end: 1210
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2424986
display id: annotation2424986
version: 1
top level: BBa_K1486010
location: https://synbiohub.org/public/igem/BBa_K1486010/annotation2424986/range2424986/1
component: BBa_B0034

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type: Range
persistent identity: range2424986
display id: range2424986
version: 1
direction: direction/reverse
top level: BBa_K1486010
start: 1211
end: 1222
orientation: inline

BBa_K1486007
type: SequenceAnnotation
persistent identity: annotation2425006
display id: annotation2425006
version: 1
top level: BBa_K1486010
location: https://synbiohub.org/public/igem/BBa_K1486010/annotation2425006/range2425006/1
component: BBa_K1486007

https://synbiohub.org/public/igem/BBa_K1486010/annotation2425006/range2425006/1
type: Range
persistent identity: range2425006
display id: range2425006
version: 1
direction: direction/reverse
top level: BBa_K1486010
start: 3104
end: 3688
orientation: inline

BBa_K338081
type: ComponentDefinition
persistent identity: BBa K338081
display id: BBa_K338081
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Heat-Shock Activated Generator
creator: Lucas Hartsough
created: 2010-10-26T11:00:00Z
modified: 2015-05-08T01:12:07Z
discontinued: false
dominant: false
group u list: _454_
m user id: 0
owner id: 6013
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Intermediate designed to accelerate cloning of the HSP promoter ( BBa_K338001 ) by adding an RBS downstream.
mutable notes: Designed to be ligated upstream of the desired heat-shock product. Keep in mind that the promoter is somewhat leaky and that cultures should be grown at 30°C.
mutable provenance: The Registry of Standard Biological Parts
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0034
component: BBa_K338001
sequence annotation: BBa_B0034
sequence annotation: BBa_K338001
sequence: https://synbiohub.org/public/igem/BBa_K338081_sequence/1

BBa_B0034
type: Component
persistent identity: component2105680
display id: component2105680
version: 1
top level: BBa_K338081
access: public
definition: BBa_B0034

BBa_K338001
type: Component
persistent identity: component2105678
display id: component2105678
version: 1
top level: BBa_K338081
access: public
definition: HSP

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2105680
display id: annotation2105680
version: 1
top level: BBa_K338081
location: https://synbiohub.org/public/igem/BBa_K338081/annotation2105680/range2105680/1
component: BBa_B0034

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type: Range
persistent identity: range2105680
display id: range2105680
version: 1
direction: direction/reverse
top level: BBa_K338081
start: 107
end: 118
orientation: inline

BBa_K338001
type: SequenceAnnotation
persistent identity: annotation2105678
display id: annotation2105678
version: 1
top level: BBa_K338081
location: https://synbiohub.org/public/igem/BBa_K338081/annotation2105678/range2105678/1
component: BBa_K338001

https://synbiohub.org/public/igem/BBa_K338081/annotation2105678/range2105678/1
type: Range
persistent identity: range2105678
display id: range2105678
version: 1
direction: direction/reverse
top level: BBa_K338081
start: 1
end: 98
orientation: inline

Pvir
type: ComponentDefinition
persistent identity: BBa K389003
display id: BBa_K389003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: virB promoter
creator: Jonas Aretz
created: 2010-08-09T11:00:00Z
modified: 2015-05-08T01:12:19Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _517_
m user id: 0
owner id: 6670
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This is a ''vir'' promotor from ''Agrobacterium tumefaciens'' C58. It is located on the TI-plasmid before the ''virB'' genes. It is activated by phosphorylated ''virG'' response regulator.
mutable notes: contains already a RBS
mutable provenance: ''A. tumefaciens'' C58, TI-plasmid
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -35
sequence annotation: rbs
sequence annotation: vir-box
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_K389003_sequence/1

-35
type: SequenceAnnotation
persistent identity: annotation2077850
display id: annotation2077850
version: 1
top level: Pvir
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K389003/annotation2077850/range2077850/1

https://synbiohub.org/public/igem/BBa_K389003/annotation2077850/range2077850/1
type: Range
persistent identity: range2077850
display id: range2077850
version: 1
direction: direction/forward
top level: Pvir
start: 89
end: 93
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation2077849
display id: annotation2077849
version: 1
top level: Pvir
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K389003/annotation2077849/range2077849/1

https://synbiohub.org/public/igem/BBa_K389003/annotation2077849/range2077849/1
type: Range
persistent identity: range2077849
display id: range2077849
version: 1
direction: direction/forward
top level: Pvir
start: 153
end: 157
orientation: inline

vir-box
type: SequenceAnnotation
persistent identity: annotation2077848
display id: annotation2077848
version: 1
top level: Pvir
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K389003/annotation2077848/range2077848/1

https://synbiohub.org/public/igem/BBa_K389003/annotation2077848/range2077848/1
type: Range
persistent identity: range2077848
display id: range2077848
version: 1
direction: direction/forward
top level: Pvir
start: 33
end: 44
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2077851
display id: annotation2077851
version: 1
top level: Pvir
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K389003/annotation2077851/range2077851/1

https://synbiohub.org/public/igem/BBa_K389003/annotation2077851/range2077851/1
type: Range
persistent identity: range2077851
display id: range2077851
version: 1
direction: direction/forward
top level: Pvir
start: 113
end: 117
orientation: inline

BBa_K873002
type: ComponentDefinition
persistent identity: BBa K873002
display id: BBa_K873002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: HSP promoter
creator: yue hu
created: 2012-09-17T11:00:00Z
modified: 2016-10-16T09:49:24Z
discontinued: false
dominant: false
experience: experience/Issues
group u list: _1134_
m user id: 29300
owner id: 11547
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This part is a heat shock promoter, it refers to the transcription factor sigma32. when the temperature rise, the transcription factor sigma32 expresses a lot and binds to the RNA ploymerase to recognize the conserved sequence in -35 and -10 region, then the promoter starts to transcript.
mutable notes: The whole sequence of this part is only 90bp, so take a futher consideration if you intend to digest it for use.
mutable provenance: We align the HSP promoters from the literature and then find the conserved sequence, then we just modified the promoter of groES of E.coli, and synthesis the part through BIC genescript.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K873002_sequence/1

BBa_J61051
type: ComponentDefinition
persistent identity: BBa J61051
display id: BBa_J61051
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: [Psal1]
creator: John Anderson
created: 2007-02-20T12:00:00Z
modified: 2015-08-31T02:03:00Z
discontinued: false
dominant: false
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Salicylate promoter and nahR
mutable notes: nonh
mutable provenance: non
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J61051_sequence/1

BBa_K106699
type: ComponentDefinition
persistent identity: BBa K106699
display id: BBa_K106699
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Gal1 Promoter
creator: Andrew Horwitz
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:09:00Z
discontinued: false
dominant: false
group u list: _226_
m user id: 0
owner id: 2659
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Galactose-inducible promoter (strong)!
mutable notes: None.
mutable provenance: Yeast genomic
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K106699_sequence/1

BBa_K101002
type: ComponentDefinition
persistent identity: BBa K101002
display id: BBa_K101002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Dual-Repressed Promoter for p22 cII and TetR
creator: Bennett Swiniarski
created: 2008-07-27T11:00:00Z
modified: 2015-05-08T01:08:41Z
discontinued: false
dominant: false
group u list: _205_
m user id: 0
owner id: 3363
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: k;kljg
mutable notes: TetR usually binds to two operator sites, one located upstream from -35 and one located between -35 and -10. However, p22 mnt also has two operator sites, one between -35 and -10 and another downstream from -10. Since each had an operator site in between -35 and -10, we had to eliminate the TetR operator site in order to allow the promoter to work. TetR was picked because it has a stronger binding than p22 mnt, and so would be more able to use only one site to bind.
mutable provenance: This part was designed by combining sequences from parts R0040 (TetR promoter) and R0053 (p22 cII promoter).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K101002_sequence/1

BBa_J56013
type: ComponentDefinition
persistent identity: BBa J56013
display id: BBa_J56013
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Rbs2 ribosome binding site
creator: Matt Eames
created: 2006-10-31T12:00:00Z
modified: 2015-08-31T03:22:59Z
discontinued: false
dominant: false
experience: experience/None
group u list: _79_98_
m user id: 0
owner id: 1132
owning group id: 98
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: ribosome binding site for transcription initiation
mutable notes: None
mutable provenance: Synthesized by DNA 2.0
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J56013_sequence/1

BBa_K598014
type: ComponentDefinition
persistent identity: BBa K598014
display id: BBa_K598014
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pBAD+HH+CdIntron+E0040+b0015
creator: Xiaowei YAN, Tong MU
created: 2011-09-26T11:00:00Z
modified: 2015-05-08T01:12:50Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _770_
m user id: 0
owner id: 8713
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This a GFP reporter regulated by a self-cleaving hammerhead ribozyme. The self-cleaving of the hammerhead ribozyme would lead to the formation of the intron P1 stem, thus triggering the self-splicing of the intron.
mutable notes: The self-cleaving of the hammerhead ribozyme would lead to the formation of the intron P1 stem, thus triggering the self-splicing of the intron.
mutable provenance: The intron derives from the genome of Cd
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
sequence annotation: RBS
sequence annotation: eGFP
sequence annotation: pBAD
sequence annotation: BBa_B0015
sequence annotation: HH
sequence annotation: group I intron
sequence: https://synbiohub.org/public/igem/BBa_K598014_sequence/1

RBS
type: SequenceAnnotation
persistent identity: annotation2153089
display id: annotation2153089
version: 1
top level: BBa_K598014
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K598014/annotation2153089/range2153089/1

https://synbiohub.org/public/igem/BBa_K598014/annotation2153089/range2153089/1
type: Range
persistent identity: range2153089
display id: range2153089
version: 1
direction: direction/forward
top level: BBa_K598014
start: 758
end: 758
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2153088
display id: annotation2153088
version: 1
top level: BBa_K598014
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K598014/annotation2153088/range2153088/1

https://synbiohub.org/public/igem/BBa_K598014/annotation2153088/range2153088/1
type: Range
persistent identity: range2153088
display id: range2153088
version: 1
direction: direction/forward
top level: BBa_K598014
start: 187
end: 191
orientation: inline

eGFP
type: SequenceAnnotation
persistent identity: annotation2153086
display id: annotation2153086
version: 1
top level: BBa_K598014
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K598014/annotation2153086/range2153086/1

https://synbiohub.org/public/igem/BBa_K598014/annotation2153086/range2153086/1
type: Range
persistent identity: range2153086
display id: range2153086
version: 1
direction: direction/forward
top level: BBa_K598014
start: 765
end: 1484
orientation: inline

pBAD
type: SequenceAnnotation
persistent identity: annotation2153081
display id: annotation2153081
version: 1
top level: BBa_K598014
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K598014/annotation2153081/range2153081/1

https://synbiohub.org/public/igem/BBa_K598014/annotation2153081/range2153081/1
type: Range
persistent identity: range2153081
display id: range2153081
version: 1
direction: direction/forward
top level: BBa_K598014
start: 1
end: 130
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2153087
display id: annotation2153087
version: 1
top level: BBa_K598014
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K598014/annotation2153087/range2153087/1

https://synbiohub.org/public/igem/BBa_K598014/annotation2153087/range2153087/1
type: Range
persistent identity: range2153087
display id: range2153087
version: 1
direction: direction/forward
top level: BBa_K598014
start: 1493
end: 1621
orientation: inline

HH
type: SequenceAnnotation
persistent identity: annotation2153082
display id: annotation2153082
version: 1
top level: BBa_K598014
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K598014/annotation2153082/range2153082/1

https://synbiohub.org/public/igem/BBa_K598014/annotation2153082/range2153082/1
type: Range
persistent identity: range2153082
display id: range2153082
version: 1
direction: direction/forward
top level: BBa_K598014
start: 139
end: 185
orientation: inline

group I intron
type: SequenceAnnotation
persistent identity: annotation2153083
display id: annotation2153083
version: 1
top level: BBa_K598014
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K598014/annotation2153083/range2153083/1

https://synbiohub.org/public/igem/BBa_K598014/annotation2153083/range2153083/1
type: Range
persistent identity: range2153083
display id: range2153083
version: 1
direction: direction/forward
top level: BBa_K598014
start: 186
end: 764
orientation: inline

BBa_K1486013
type: ComponentDefinition
persistent identity: BBa K1486013
display id: BBa_K1486013
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CpxR-IFP[2] under pBAD
creator: iGem EPFL 2014
created: 2014-08-31T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part has a fused protein downstream of an arabinose-inducible promoter: CpxR-IFP[2]. Biobrick BBa_K1486012 has CpxR-IFP[1] with an arabinose promoter. This construct was made in both orientations: BBa_K1486015 is IFP[2]-CpxR (with an arabinose promoter).
mutable notes: Both orientations were made, as well as for IFP[1] so all dimerizations could be tested (see main page for biobrick numbers). Flexible linkers were used to fuse both proteins, and an RBS was put before each one of them.
mutable provenance: to edit
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0034
component: BBa_I0500
component: BBa_K1486000
component: BBa_K1486004
component: BBa_K1486007
sequence annotation: BBa_K1486007
sequence annotation: BBa_I0500
sequence annotation: BBa_B0034
sequence annotation: BBa_K1486000
sequence annotation: BBa_K1486004
sequence: https://synbiohub.org/public/igem/BBa_K1486013_sequence/1

BBa_B0034
type: Component
persistent identity: component2382299
display id: component2382299
version: 1
top level: BBa_K1486013
access: public
definition: BBa_B0034

BBa_I0500
type: Component
persistent identity: component2382297
display id: component2382297
version: 1
top level: BBa_K1486013
access: public
definition: pBad/araC

BBa_K1486000
type: Component
persistent identity: component2382303
display id: component2382303
version: 1
top level: BBa_K1486013
access: public
definition: BBa_K1486000

BBa_K1486004
type: Component
persistent identity: component2382304
display id: component2382304
version: 1
top level: BBa_K1486013
access: public
definition: BBa_K1486004

BBa_K1486007
type: Component
persistent identity: component2382308
display id: component2382308
version: 1
top level: BBa_K1486013
access: public
definition: BBa_K1486007

BBa_K1486007
type: SequenceAnnotation
persistent identity: annotation2382308
display id: annotation2382308
version: 1
top level: BBa_K1486013
location: https://synbiohub.org/public/igem/BBa_K1486013/annotation2382308/range2382308/1
component: BBa_K1486007

https://synbiohub.org/public/igem/BBa_K1486013/annotation2382308/range2382308/1
type: Range
persistent identity: range2382308
display id: range2382308
version: 1
direction: direction/reverse
top level: BBa_K1486013
start: 1955
end: 2533
orientation: inline

BBa_I0500
type: SequenceAnnotation
persistent identity: annotation2382297
display id: annotation2382297
version: 1
top level: BBa_K1486013
location: https://synbiohub.org/public/igem/BBa_K1486013/annotation2382297/range2382297/1
component: BBa_I0500

https://synbiohub.org/public/igem/BBa_K1486013/annotation2382297/range2382297/1
type: Range
persistent identity: range2382297
display id: range2382297
version: 1
direction: direction/reverse
top level: BBa_K1486013
start: 1
end: 1210
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2382299
display id: annotation2382299
version: 1
top level: BBa_K1486013
location: https://synbiohub.org/public/igem/BBa_K1486013/annotation2382299/range2382299/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1486013/annotation2382299/range2382299/1
type: Range
persistent identity: range2382299
display id: range2382299
version: 1
direction: direction/reverse
top level: BBa_K1486013
start: 1211
end: 1222
orientation: inline

BBa_K1486000
type: SequenceAnnotation
persistent identity: annotation2382303
display id: annotation2382303
version: 1
top level: BBa_K1486013
location: https://synbiohub.org/public/igem/BBa_K1486013/annotation2382303/range2382303/1
component: BBa_K1486000

https://synbiohub.org/public/igem/BBa_K1486013/annotation2382303/range2382303/1
type: Range
persistent identity: range2382303
display id: range2382303
version: 1
direction: direction/reverse
top level: BBa_K1486013
start: 1223
end: 1924
orientation: inline

BBa_K1486004
type: SequenceAnnotation
persistent identity: annotation2382304
display id: annotation2382304
version: 1
top level: BBa_K1486013
location: https://synbiohub.org/public/igem/BBa_K1486013/annotation2382304/range2382304/1
component: BBa_K1486004

https://synbiohub.org/public/igem/BBa_K1486013/annotation2382304/range2382304/1
type: Range
persistent identity: range2382304
display id: range2382304
version: 1
direction: direction/reverse
top level: BBa_K1486013
start: 1925
end: 1954
orientation: inline

PgsiB
type: ComponentDefinition
persistent identity: BBa K143011
display id: BBa_K143011
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter gsiB for B. subtilis
creator: James Chappell
created: 2008-09-10T11:00:00Z
modified: 2015-05-08T01:10:23Z
discontinued: false
dominant: false
experience: experience/None
group u list: _199_
m user id: 0
owner id: 2090
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Promoter gsiB is a sigma factor B dependent promoter found in ''B.subtilis''. In ''B.subtilis'' endogenous sigma factor B is activated under mild stress. These mild stress conditions can be generally split into nutrient stress response and physical stress response. Nutrient stress response is triggered by low levels of ATP and GTP and physical stress response is triggered by exposure to blue light, salt, heat, acid or ethanol1. The promoter gsiB has been used previously as a read out for the activation of sigma factor B 2. The context with which we used the promoter gsiB, was to take blue light as an input and give '''Polymerase Per Second'''(PoPS) as an output. To do this the other potential inputs need to be carefully controlled so that only blue light activated the sigma B and gives a PoPS output. In order to get sufficient sigma B activation by blue light the light receptor YtvA, part...., needs to be over expressed in ''B.subtilis'' 3.
mutable notes: Biobrick standard was applied to the promoter gsiB sequence.
mutable provenance: The part was designed using the sequence from the ''B.subtilis'' genome and from previously published papers 23. This sequence was then synthesised by Geneart.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: Sigma B -35
sequence annotation: Sigma B -10
sequence: https://synbiohub.org/public/igem/BBa_K143011_sequence/1

Sigma B -35
type: SequenceAnnotation
persistent identity: annotation1975702
display id: annotation1975702
version: 1
top level: PgsiB
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K143011/annotation1975702/range1975702/1

https://synbiohub.org/public/igem/BBa_K143011/annotation1975702/range1975702/1
type: Range
persistent identity: range1975702
display id: range1975702
version: 1
direction: direction/forward
top level: PgsiB
start: 6
end: 11
orientation: inline

Sigma B -10
type: SequenceAnnotation
persistent identity: annotation1975703
display id: annotation1975703
version: 1
top level: PgsiB
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K143011/annotation1975703/range1975703/1

https://synbiohub.org/public/igem/BBa_K143011/annotation1975703/range1975703/1
type: Range
persistent identity: range1975703
display id: range1975703
version: 1
direction: direction/forward
top level: PgsiB
start: 25
end: 30
orientation: inline

BBa_J64028
type: ComponentDefinition
persistent identity: BBa J64028
display id: BBa_J64028
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: sopB RBS
creator: Dan Widmaier
created: 2008-01-16T12:00:00Z
modified: 2015-08-31T01:56:26Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _98_
m user id: 0
owner id: 2407
owning group id: 98
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This part is the natural RBS for the sopB gene in the Salmonella Type III Secretion System.
mutable notes: This part was not designed with consideration for biobrick standard assembly in mind. This RBS hasn't been explicitly tested for expression rate in a Fluorescence or B-Gal assay.
mutable provenance: Salmonella LT2 Genomic DNA
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: sopB RBS
sequence: https://synbiohub.org/public/igem/BBa_J64028_sequence/1

sopB RBS
type: SequenceAnnotation
persistent identity: annotation1959632
display id: annotation1959632
version: 1
top level: BBa_J64028
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_J64028/annotation1959632/range1959632/1

https://synbiohub.org/public/igem/BBa_J64028/annotation1959632/range1959632/1
type: Range
persistent identity: range1959632
display id: range1959632
version: 1
direction: direction/forward
top level: BBa_J64028
start: 1
end: 20
orientation: inline

BBa_K180000
type: ComponentDefinition
persistent identity: BBa K180000
display id: BBa_K180000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Hybrid promoter (trp & lac regulated -- tac pR)
creator: Richard Wilson, Stephanie Chambers
created: 2009-10-11T11:00:00Z
modified: 2015-05-08T01:11:06Z
discontinued: false
dominant: false
experience: experience/None
group u list: _278_
m user id: 0
owner id: 4082
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: The Ptac promoter is a functional hybrid promoter, derived from the trp and lac promoters, that are regulated by trp and lac [1]. The Ptac sequence has been extracted by PCR from the commercially available plasmid pMAL-pIII from New England Biolabs (N8101S), with the addition of the biobrick prefix and suffix sequences. [1] Proc. Natl. Acad. Sci. USA, Vol. 80, pp. 21-25
mutable notes: Sequence includes lacIq and Shine-Dalgarno sequence from pMAL-pIII prior to the Ptac promoter to ensure correct functionality of the promoter.
mutable provenance: pMAL-pIII (New England Biolabs N8101S)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: lacIq
sequence annotation: Ptac
sequence: https://synbiohub.org/public/igem/BBa_K180000_sequence/1

lacIq
type: SequenceAnnotation
persistent identity: annotation2044120
display id: annotation2044120
version: 1
top level: BBa_K180000
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K180000/annotation2044120/range2044120/1

https://synbiohub.org/public/igem/BBa_K180000/annotation2044120/range2044120/1
type: Range
persistent identity: range2044120
display id: range2044120
version: 1
direction: direction/forward
top level: BBa_K180000
start: 1
end: 1082
orientation: inline

Ptac
type: SequenceAnnotation
persistent identity: annotation2044121
display id: annotation2044121
version: 1
top level: BBa_K180000
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K180000/annotation2044121/range2044121/1

https://synbiohub.org/public/igem/BBa_K180000/annotation2044121/range2044121/1
type: Range
persistent identity: range2044121
display id: range2044121
version: 1
direction: direction/forward
top level: BBa_K180000
start: 1325
end: 1352
orientation: inline

p(tetR)
type: ComponentDefinition
persistent identity: BBa R0040
display id: BBa_R0040
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TetR repressible promoter
creator: June Rhee, Connie Tao, Ty Thomson, Louis Waldman
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Sequence for pTet inverting regulator driven by the TetR protein.
mutable notes: BBa_R0040 TetR-Regulated Promoter is based on a cI promoter. It has been modified to include two TetR binding sites and the BioBrick standard assembly head and tail restriction sites.
mutable provenance: Lutz, R., Bujard, H., Nucleic Acids Research (1997) 25, 1203-1210.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: BBa_R0040
sequence annotation: TetR 1
sequence annotation: -10
sequence annotation: -35
sequence annotation: TetR 2
sequence: https://synbiohub.org/public/igem/BBa_R0040_sequence/1

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1986784
display id: annotation1986784
version: 1
top level: p(tetR)
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986784/range1986784/1

https://synbiohub.org/public/igem/BBa_R0040/annotation1986784/range1986784/1
type: Range
persistent identity: range1986784
display id: range1986784
version: 1
direction: direction/forward
top level: p(tetR)
start: 1
end: 54
orientation: inline

TetR 1
type: SequenceAnnotation
persistent identity: annotation1986783
display id: annotation1986783
version: 1
top level: p(tetR)
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986783/range1986783/1

https://synbiohub.org/public/igem/BBa_R0040/annotation1986783/range1986783/1
type: Range
persistent identity: range1986783
display id: range1986783
version: 1
direction: direction/forward
top level: p(tetR)
start: 1
end: 19
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1986787
display id: annotation1986787
version: 1
top level: p(tetR)
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986787/range1986787/1

https://synbiohub.org/public/igem/BBa_R0040/annotation1986787/range1986787/1
type: Range
persistent identity: range1986787
display id: range1986787
version: 1
direction: direction/forward
top level: p(tetR)
start: 43
end: 48
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1986785
display id: annotation1986785
version: 1
top level: p(tetR)
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986785/range1986785/1

https://synbiohub.org/public/igem/BBa_R0040/annotation1986785/range1986785/1
type: Range
persistent identity: range1986785
display id: range1986785
version: 1
direction: direction/forward
top level: p(tetR)
start: 20
end: 25
orientation: inline

TetR 2
type: SequenceAnnotation
persistent identity: annotation1986786
display id: annotation1986786
version: 1
top level: p(tetR)
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986786/range1986786/1

https://synbiohub.org/public/igem/BBa_R0040/annotation1986786/range1986786/1
type: Range
persistent identity: range1986786
display id: range1986786
version: 1
direction: direction/forward
top level: p(tetR)
start: 26
end: 44
orientation: inline

BBa_B0077
type: ComponentDefinition
persistent identity: BBa B0077
display id: BBa_B0077
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Specialized RBS
creator: Bartholomew Canton
created: 2006-05-01T11:00:00Z
modified: 2015-08-31T04:07:21Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_6_
m user id: 0
owner id: 135
owning group id: 6
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_B0077_sequence/1

BBa_K1486020
type: ComponentDefinition
persistent identity: BBa K1486020
display id: BBa_K1486020
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: rLuc[2]
creator: EPFL iGem team 2014
created: 2014-09-14T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is the second part of the split Renilla luciferase. To be used with rLuc[1].
mutable notes: to edit
mutable provenance: to edit
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence: https://synbiohub.org/public/igem/BBa_K1486020_sequence/1

BBa_K1480003
type: ComponentDefinition
persistent identity: BBa K1480003
display id: BBa_K1480003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TetR generator (Promoter J23109)
creator: Jesus Gilberto Rodriguez Ceja
created: 2014-10-13T11:00:00Z
modified: 2015-05-08T01:10:40Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1860_
m user id: 0
owner id: 12878
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is a TetR protein generator regulated by the constitutive promoter J23109. The protein has a LVA tag for degradation.
mutable notes: It was assembled using assembly 10.
mutable provenance: Based on parts of the registry.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0034
component: BBa_J23109
component: BBa_B0012
component: BBa_C0040
component: BBa_B0010
sequence annotation: BBa_B0034
sequence annotation: BBa_C0040
sequence annotation: BBa_B0012
sequence annotation: BBa_B0010
sequence annotation: BBa_J23109
sequence: https://synbiohub.org/public/igem/BBa_K1480003_sequence/1

BBa_B0034
type: Component
persistent identity: component2419530
display id: component2419530
version: 1
top level: BBa_K1480003
access: public
definition: BBa_B0034

BBa_J23109
type: Component
persistent identity: component2419528
display id: component2419528
version: 1
top level: BBa_K1480003
access: public
definition: BBa_J23109

BBa_B0012
type: Component
persistent identity: component2419537
display id: component2419537
version: 1
top level: BBa_K1480003
access: public
definition: BBa_B0012

BBa_C0040
type: Component
persistent identity: component2419534
display id: component2419534
version: 1
top level: BBa_K1480003
access: public
definition: https://synbiohub.org/public/igem/BBa_C0040/1

BBa_B0010
type: Component
persistent identity: component2419535
display id: component2419535
version: 1
top level: BBa_K1480003
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2419530
display id: annotation2419530
version: 1
top level: BBa_K1480003
location: https://synbiohub.org/public/igem/BBa_K1480003/annotation2419530/range2419530/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1480003/annotation2419530/range2419530/1
type: Range
persistent identity: range2419530
display id: range2419530
version: 1
direction: direction/reverse
top level: BBa_K1480003
start: 44
end: 55
orientation: inline

BBa_C0040
type: SequenceAnnotation
persistent identity: annotation2419534
display id: annotation2419534
version: 1
top level: BBa_K1480003
location: https://synbiohub.org/public/igem/BBa_K1480003/annotation2419534/range2419534/1
component: BBa_C0040

https://synbiohub.org/public/igem/BBa_K1480003/annotation2419534/range2419534/1
type: Range
persistent identity: range2419534
display id: range2419534
version: 1
direction: direction/reverse
top level: BBa_K1480003
start: 62
end: 746
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2419537
display id: annotation2419537
version: 1
top level: BBa_K1480003
location: https://synbiohub.org/public/igem/BBa_K1480003/annotation2419537/range2419537/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K1480003/annotation2419537/range2419537/1
type: Range
persistent identity: range2419537
display id: range2419537
version: 1
direction: direction/forward
top level: BBa_K1480003
start: 843
end: 883
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2419535
display id: annotation2419535
version: 1
top level: BBa_K1480003
location: https://synbiohub.org/public/igem/BBa_K1480003/annotation2419535/range2419535/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K1480003/annotation2419535/range2419535/1
type: Range
persistent identity: range2419535
display id: range2419535
version: 1
direction: direction/forward
top level: BBa_K1480003
start: 755
end: 834
orientation: inline

BBa_J23109
type: SequenceAnnotation
persistent identity: annotation2419528
display id: annotation2419528
version: 1
top level: BBa_K1480003
location: https://synbiohub.org/public/igem/BBa_K1480003/annotation2419528/range2419528/1
component: BBa_J23109

https://synbiohub.org/public/igem/BBa_K1480003/annotation2419528/range2419528/1
type: Range
persistent identity: range2419528
display id: range2419528
version: 1
direction: direction/reverse
top level: BBa_K1480003
start: 1
end: 35
orientation: inline

pBad/araC
type: ComponentDefinition
persistent identity: BBa I0500
display id: BBa_I0500
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Inducible pBad/araC promoter
creator: Sri Kosuri
created: 2003-12-05T12:00:00Z
modified: 2015-08-31T04:07:29Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: AraC
sequence annotation: PC
sequence annotation: Pbad
sequence: https://synbiohub.org/public/igem/BBa_I0500_sequence/1

AraC
type: SequenceAnnotation
persistent identity: annotation1498843
display id: annotation1498843
version: 1
top level: pBad/araC
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I0500/annotation1498843/range1498843/1

https://synbiohub.org/public/igem/BBa_I0500/annotation1498843/range1498843/1
type: Range
persistent identity: range1498843
display id: range1498843
version: 1
direction: direction/forward
top level: pBad/araC
start: 1
end: 1079
orientation: inline

PC
type: SequenceAnnotation
persistent identity: annotation1498842
display id: annotation1498842
version: 1
top level: pBad/araC
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I0500/annotation1498842/range1498842/1

https://synbiohub.org/public/igem/BBa_I0500/annotation1498842/range1498842/1
type: Range
persistent identity: range1498842
display id: range1498842
version: 1
direction: direction/forward
top level: pBad/araC
start: 1043
end: 1114
orientation: inline

Pbad
type: SequenceAnnotation
persistent identity: annotation1498841
display id: annotation1498841
version: 1
top level: pBad/araC
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I0500/annotation1498841/range1498841/1

https://synbiohub.org/public/igem/BBa_I0500/annotation1498841/range1498841/1
type: Range
persistent identity: range1498841
display id: range1498841
version: 1
direction: direction/forward
top level: pBad/araC
start: 1080
end: 1210
orientation: inline

BBa_I11013
type: ComponentDefinition
persistent identity: BBa I11013
display id: BBa_I11013
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Modification of biobricks part BBa_B0015
creator: bu
created: 2004-09-02T11:00:00Z
modified: 2015-08-31T04:07:30Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2_
m user id: 0
owner id: 102
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: Modified BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_I11013_sequence/1

Modified BBa_B0015
type: SequenceAnnotation
persistent identity: annotation1064099
display id: annotation1064099
version: 1
top level: BBa_I11013
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_I11013/annotation1064099/range1064099/1

https://synbiohub.org/public/igem/BBa_I11013/annotation1064099/range1064099/1
type: Range
persistent identity: range1064099
display id: range1064099
version: 1
direction: direction/forward
top level: BBa_I11013
start: 1
end: 129
orientation: inline

BBa_K150005
type: ComponentDefinition
persistent identity: BBa K150005
display id: BBa_K150005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: ribosome binding site of pTrc99a
creator: Pascal Kraemer
created: 2008-10-20T11:00:00Z
modified: 2015-05-08T01:10:46Z
discontinued: false
dominant: false
experience: experience/None
group u list: _234_
m user id: 0
owner id: 3299
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: ribosome binding site of pTrc99a
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: BBa_K150005
sequence: https://synbiohub.org/public/igem/BBa_K150005_sequence/1

BBa_K150005
type: SequenceAnnotation
persistent identity: annotation1983190
display id: annotation1983190
version: 1
top level: BBa_K150005
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K150005/annotation1983190/range1983190/1

https://synbiohub.org/public/igem/BBa_K150005/annotation1983190/range1983190/1
type: Range
persistent identity: range1983190
display id: range1983190
version: 1
direction: direction/forward
top level: BBa_K150005
start: 1
end: 13
orientation: inline

U061O11+D0
type: ComponentDefinition
persistent identity: BBa I732433
display id: BBa_I732433
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Family Member (U061O11+D026O22)
creator: Zhan Jian
created: 2007-10-13T11:00:00Z
modified: 2015-08-31T04:07:58Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732433_sequence/1

GFP-GOI
type: ComponentDefinition
persistent identity: BBa K678028
display id: BBa_K678028
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: GFP-GOI
creator: DTU-Denmark-2
created: 2011-09-10T11:00:00Z
modified: 2015-05-08T01:13:04Z
discontinued: false
dominant: false
experience: experience/None
group u list: _882_
m user id: 0
owner id: 8341
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: GFP for Aspergillus nidulans
mutable notes: To be announced
mutable provenance: Design considerations This part was designed for the Plug'n'Play with DNA assembly standard (RFC 80), therefore this part contains linkers at the 5' and 3' ends as shown on the figure below.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: GFP
sequence annotation: Linker 2
sequence: https://synbiohub.org/public/igem/BBa_K678028_sequence/1

GFP
type: SequenceAnnotation
persistent identity: annotation2127385
display id: annotation2127385
version: 1
top level: GFP-GOI
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K678028/annotation2127385/range2127385/1

https://synbiohub.org/public/igem/BBa_K678028/annotation2127385/range2127385/1
type: Range
persistent identity: range2127385
display id: range2127385
version: 1
direction: direction/forward
top level: GFP-GOI
start: 9
end: 725
orientation: inline

Linker 2
type: SequenceAnnotation
persistent identity: annotation2127384
display id: annotation2127384
version: 1
top level: GFP-GOI
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K678028/annotation2127384/range2127384/1

https://synbiohub.org/public/igem/BBa_K678028/annotation2127384/range2127384/1
type: Range
persistent identity: range2127384
display id: range2127384
version: 1
direction: direction/forward
top level: GFP-GOI
start: 1
end: 8
orientation: inline

BBa_I739102
type: ComponentDefinition
persistent identity: BBa I739102
display id: BBa_I739102
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Double Promoter (cI, negative / TetR, negative)
creator: Stefan Luzi
created: 2007-10-13T11:00:00Z
modified: 2015-08-31T04:08:01Z
discontinued: false
dominant: false
group u list: _124_
m user id: 0
owner id: 2183
owning group id: 62
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: asdf
mutable notes: asdf
mutable provenance: Synthetic DNA
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: R0051
sequence annotation: Scar
sequence annotation: TetR 1
sequence annotation: R0040 (2x TetR 1)
sequence: https://synbiohub.org/public/igem/BBa_I739102_sequence/1

R0051
type: SequenceAnnotation
persistent identity: annotation1946930
display id: annotation1946930
version: 1
top level: BBa_I739102
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I739102/annotation1946930/range1946930/1

https://synbiohub.org/public/igem/BBa_I739102/annotation1946930/range1946930/1
type: Range
persistent identity: range1946930
display id: range1946930
version: 1
direction: direction/forward
top level: BBa_I739102
start: 1
end: 49
orientation: inline

Scar
type: SequenceAnnotation
persistent identity: annotation1946931
display id: annotation1946931
version: 1
top level: BBa_I739102
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I739102/annotation1946931/range1946931/1

https://synbiohub.org/public/igem/BBa_I739102/annotation1946931/range1946931/1
type: Range
persistent identity: range1946931
display id: range1946931
version: 1
direction: direction/forward
top level: BBa_I739102
start: 50
end: 57
orientation: inline

TetR 1
type: SequenceAnnotation
persistent identity: annotation1946932
display id: annotation1946932
version: 1
top level: BBa_I739102
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I739102/annotation1946932/range1946932/1

https://synbiohub.org/public/igem/BBa_I739102/annotation1946932/range1946932/1
type: Range
persistent identity: range1946932
display id: range1946932
version: 1
direction: direction/forward
top level: BBa_I739102
start: 78
end: 97
orientation: inline

R0040 (2x TetR 1)
type: SequenceAnnotation
persistent identity: annotation1946933
display id: annotation1946933
version: 1
top level: BBa_I739102
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_I739102/annotation1946933/range1946933/1

https://synbiohub.org/public/igem/BBa_I739102/annotation1946933/range1946933/1
type: Range
persistent identity: range1946933
display id: range1946933
version: 1
direction: direction/forward
top level: BBa_I739102
start: 58
end: 97
orientation: inline

TetR 1
type: SequenceAnnotation
persistent identity: annotation1946934
display id: annotation1946934
version: 1
top level: BBa_I739102
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I739102/annotation1946934/range1946934/1

https://synbiohub.org/public/igem/BBa_I739102/annotation1946934/range1946934/1
type: Range
persistent identity: range1946934
display id: range1946934
version: 1
direction: direction/forward
top level: BBa_I739102
start: 58
end: 77
orientation: inline

ptrA
type: ComponentDefinition
persistent identity: BBa K678045
display id: BBa_K678045
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: ptrA, selection marker for Aspergillus nidulans
creator: DTU-Denmark-2
created: 2011-09-04T11:00:00Z
modified: 2015-05-08T01:13:04Z
discontinued: false
dominant: false
experience: experience/None
group u list: _882_
m user id: 0
owner id: 8341
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: ptrA, selection marker for Aspergillus nidulans
mutable notes: Compatible with the Plug'n'Play assembly standard
mutable provenance: ptrA
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Linker 5
sequence annotation: ptrA cassette
sequence: https://synbiohub.org/public/igem/BBa_K678045_sequence/1

Linker 5
type: SequenceAnnotation
persistent identity: annotation2127421
display id: annotation2127421
version: 1
top level: ptrA
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K678045/annotation2127421/range2127421/1

https://synbiohub.org/public/igem/BBa_K678045/annotation2127421/range2127421/1
type: Range
persistent identity: range2127421
display id: range2127421
version: 1
direction: direction/forward
top level: ptrA
start: 1
end: 8
orientation: inline

ptrA cassette
type: SequenceAnnotation
persistent identity: annotation2127422
display id: annotation2127422
version: 1
top level: ptrA
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K678045/annotation2127422/range2127422/1

https://synbiohub.org/public/igem/BBa_K678045/annotation2127422/range2127422/1
type: Range
persistent identity: range2127422
display id: range2127422
version: 1
direction: direction/forward
top level: ptrA
start: 9
end: 1899
orientation: inline

BBa_J52016
type: ComponentDefinition
persistent identity: BBa J52016
display id: BBa_J52016
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: eukaryotic -- derived from SV40 early poly A signal sequence
creator: Monika Ciglic
created: 2006-10-13T11:00:00Z
modified: 2015-08-31T03:54:04Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _80_
m user id: 0
owner id: 800
owning group id: 80
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: -
mutable notes: It is cloned in BioBrick vector pSB1AK3.
mutable provenance: -
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: terminator
sequence: https://synbiohub.org/public/igem/BBa_J52016_sequence/1

terminator
type: SequenceAnnotation
persistent identity: annotation1902870
display id: annotation1902870
version: 1
top level: BBa_J52016
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_J52016/annotation1902870/range1902870/1

https://synbiohub.org/public/igem/BBa_J52016/annotation1902870/range1902870/1
type: Range
persistent identity: range1902870
display id: range1902870
version: 1
direction: direction/forward
top level: BBa_J52016
start: 1
end: 238
orientation: inline

terminator
type: SequenceAnnotation
persistent identity: annotation1902869
display id: annotation1902869
version: 1
top level: BBa_J52016
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_J52016/annotation1902869/range1902869/1

https://synbiohub.org/public/igem/BBa_J52016/annotation1902869/range1902869/1
type: Range
persistent identity: range1902869
display id: range1902869
version: 1
direction: direction/forward
top level: BBa_J52016
start: 1
end: 238
orientation: inline

BBa_J61114
type: ComponentDefinition
persistent identity: BBa J61114
display id: BBa_J61114
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Ribosome Binding Site Family Member
creator: John Anderson
created: 2007-04-23T11:00:00Z
modified: 2015-08-31T02:03:00Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: false
mutable description: {{JCA_Arkin_RBSFamily}}
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J61114_sequence/1

BBa_I756021
type: ComponentDefinition
persistent identity: BBa I756021
display id: BBa_I756021
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CMV promoter with Lambda Operator
creator: Jack
created: 2007-08-15T11:00:00Z
modified: 2015-08-31T04:08:06Z
discontinued: false
dominant: false
group u list: _108_
m user id: 0
owner id: 2067
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: CMV promoter with Lambda Operator
mutable notes: CMV promoter with Lambda Operator
mutable provenance: CMV promoter with Lambda Operator
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I756021_sequence/1

BBa_K1660007
type: ComponentDefinition
persistent identity: BBa K1660007
display id: BBa_K1660007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PompR-6N-gp35-Terminator
creator: Dai Yuanyi
created: 2015-09-07T11:00:00Z
modified: 2015-09-08T12:46:10Z
discontinued: false
dominant: false
group u list: _2078_
m user id: 26933
owner id: 26933
owning group id: 9
status: status/Planning
bookmark: false
mutable description: PompR is a OmpR-controlled promoter which can be positively regulated by phosphorylated OmpR. This promoter is taken from the upstream region of ompC. Phosphorylated OmpR binds to the three operator sites and activates transcription. We insert PompR(BBa_R0082) sequences into the upstream region of our target gene gp35 through restriction-ligation method, which together is connected to vector PSB1C3 afterwards, and therefore we are able to regulate the expression of in through the control of light. Bxb1 gp35 is a serine integrase in Mycobacterium phage Bxb1. This intergrase can exclusively catalyze site-specific recombination. The dismiss of light singal can drive the expression of integrase, which alone can set a DNA sequence(there it means the promoter) flanked by oppositional attB and attP sites, thereby produce an inverted sequence flanked by attL and attR sites. As a consequence, the promotor moves on to express the toxic protein instead of the attractant. We insert the gp35 gene into the downstream region of the PompR promotor by means of 3A synthesis so that the expression of integrase can be regulated by light. In addition, we add a 6N promotor between PompR and gp35 to decrease basal integrase expression above flipping threshold level which could lower the efficiency of int translation. Meanwhile, we insert the recombined sites of attB and attP into the flanking sequences of our constitutive promotor (BBa_J23110 ) and so far we accomplish our reverse promotor system.
mutable notes: we add a 6N promotor between PompR and gp35 to decrease basal integrase expression above flipping threshold level which could lower the efficiency of int translation.
mutable provenance: It comes from the register.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_R0082
component: BBa_K1433008
sequence annotation: BBa_R0082
sequence annotation: BBa_K1433008
sequence: https://synbiohub.org/public/igem/BBa_K1660007_sequence/1

BBa_R0082
type: Component
persistent identity: component2446518
display id: component2446518
version: 1
top level: BBa_K1660007
access: public
definition: OmpR

BBa_K1433008
type: Component
persistent identity: component2446523
display id: component2446523
version: 1
top level: BBa_K1660007
access: public
definition: https://synbiohub.org/public/igem/BBa_K1433008/1

BBa_R0082
type: SequenceAnnotation
persistent identity: annotation2446518
display id: annotation2446518
version: 1
top level: BBa_K1660007
location: https://synbiohub.org/public/igem/BBa_K1660007/annotation2446518/range2446518/1
component: BBa_R0082

https://synbiohub.org/public/igem/BBa_K1660007/annotation2446518/range2446518/1
type: Range
persistent identity: range2446518
display id: range2446518
version: 1
direction: direction/reverse
top level: BBa_K1660007
start: 1
end: 108
orientation: inline

BBa_K1433008
type: SequenceAnnotation
persistent identity: annotation2446523
display id: annotation2446523
version: 1
top level: BBa_K1660007
location: https://synbiohub.org/public/igem/BBa_K1660007/annotation2446523/range2446523/1
component: BBa_K1433008

https://synbiohub.org/public/igem/BBa_K1660007/annotation2446523/range2446523/1
type: Range
persistent identity: range2446523
display id: range2446523
version: 1
direction: direction/reverse
top level: BBa_K1660007
start: 117
end: 1773
orientation: inline

BBa_I746364
type: ComponentDefinition
persistent identity: BBa I746364
display id: BBa_I746364
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Psid promoter from P4 phage
creator: Stefan Milde
created: 2007-09-10T11:00:00Z
modified: 2015-08-31T04:08:04Z
discontinued: false
dominant: false
group u list: _116_
m user id: 0
owner id: 2122
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This is the Psid promoter taken from the P4 phage genome. It is an inducible promoter that is activated by a class of activators, including P2 ogr (I746350), PSP3 pag (I746351) and phiR73 delta (I746352). These different activators should cause different levels of activity of the Psid promoter.
mutable notes: no special considerations
mutable provenance: P4 phage genome
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: Psid
sequence: https://synbiohub.org/public/igem/BBa_I746364_sequence/1

Psid
type: SequenceAnnotation
persistent identity: annotation1943787
display id: annotation1943787
version: 1
top level: BBa_I746364
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I746364/annotation1943787/range1943787/1

https://synbiohub.org/public/igem/BBa_I746364/annotation1943787/range1943787/1
type: Range
persistent identity: range1943787
display id: range1943787
version: 1
direction: direction/forward
top level: BBa_I746364
start: 1
end: 93
orientation: inline

BBa_K1886017
type: ComponentDefinition
persistent identity: BBa K1886017
display id: BBa_K1886017
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: broken_Ptet
creator: Lejian Jiang
created: 2016-10-11T11:00:00Z
modified: 2016-10-17T11:34:11Z
discontinued: false
dominant: false
group u list: _2351_
m user id: 29387
owner id: 29387
owning group id: 9
status: status/Planning
bookmark: false
mutable description: xxx
mutable notes: xxx
mutable provenance: xxx
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K1886017_sequence/1

BBa_K082000
type: ComponentDefinition
persistent identity: BBa K082000
display id: BBa_K082000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: reconstruct strong RBS
creator: Yanting Xue , Binjie Xu
created: 2008-10-27T12:00:00Z
modified: 2015-05-08T01:08:35Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _228_
m user id: 0
owner id: 1560
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Strong RBS based on Ron Weiss thesis. Strength is considered relative to BBa_B0031, BBa_B0032, BBa_B0033 and BBa_B0034.
mutable notes: The
mutable provenance: The
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: BBa_K082000
sequence: https://synbiohub.org/public/igem/BBa_K082000_sequence/1

BBa_K082000
type: SequenceAnnotation
persistent identity: annotation1992768
display id: annotation1992768
version: 1
top level: BBa_K082000
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K082000/annotation1992768/range1992768/1

https://synbiohub.org/public/igem/BBa_K082000/annotation1992768/range1992768/1
type: Range
persistent identity: range1992768
display id: range1992768
version: 1
direction: direction/reverse
top level: BBa_K082000
start: 1
end: 17
orientation: inline

Pnot(O55)
type: ComponentDefinition
persistent identity: BBa I732205
display id: BBa_I732205
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: NOT Gate Promoter Family Member (D001O55)
creator: Zhan Jian
created: 2007-10-13T11:00:00Z
modified: 2015-08-31T04:07:57Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732205_sequence/1

BBa_R0185
type: ComponentDefinition
persistent identity: BBa R0185
display id: BBa_R0185
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: T7 promoter (lacI repressible)
creator: Bartholomew Canton
created: 2008-04-07T11:00:00Z
modified: 2015-05-08T01:14:15Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_
m user id: 0
owner id: 135
owning group id: 84
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: A lac repressible T7 promoter. This promoter is similar to those found in the pET vectors with T7lac promoters. I mutated the -10 base to a G, which should make it about 4% of the strength of consensus. The base numbering is as used by Studier and coworkers (JMB 1991, 219, 45-59). See BBa_R0183 for more information.
mutable notes: The -10 mutation from consensus was included to weaken the consensus promoter as described by Imburgio and co-workers (see BBa_R0183 for more information)
mutable provenance: This sequence is based on the T7lac promoter described by Dubendorff and Studier (JMB 1991, 219, 45-59). The only modification is the mutation at -10.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: T7 promoter sequence
sequence annotation: LacI binding site
sequence: https://synbiohub.org/public/igem/BBa_R0185_sequence/1

T7 promoter sequence
type: SequenceAnnotation
persistent identity: annotation1962092
display id: annotation1962092
version: 1
top level: BBa_R0185
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0185/annotation1962092/range1962092/1

https://synbiohub.org/public/igem/BBa_R0185/annotation1962092/range1962092/1
type: Range
persistent identity: range1962092
display id: range1962092
version: 1
direction: direction/forward
top level: BBa_R0185
start: 1
end: 20
orientation: inline

LacI binding site
type: SequenceAnnotation
persistent identity: annotation1962093
display id: annotation1962093
version: 1
top level: BBa_R0185
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0185/annotation1962093/range1962093/1

https://synbiohub.org/public/igem/BBa_R0185/annotation1962093/range1962093/1
type: Range
persistent identity: range1962093
display id: range1962093
version: 1
direction: direction/forward
top level: BBa_R0185
start: 20
end: 44
orientation: inline

cI 434
type: ComponentDefinition
persistent identity: BBa R1052
display id: BBa_R1052
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter, Standard (434 cI regulated)
creator: Maia Mahoney, Drew Endy
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:15Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: [Note: This is the same part as R0052 except that the -10 and -35 sites and spacing have been changed to comply with BBa_S0001]. The 434 cI regulatory region sequence is a 89 base-pair sequence with the standard BioBrick prefix and suffix sections on its ends. 434 cI repressor protein, <bb_part>BBa_C0052<bb_part>, binds to it. This segment contains O-R1, O-R2, P-R, and P-RM -35.
mutable notes: Wild-type promoter modified to comply with BBa_S0001: TTGACA-17N-GATACT
mutable provenance: Bacteriophage 434 right operator
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: OR1
sequence annotation: -35
sequence annotation: start
sequence annotation: -10
sequence annotation: OR2
sequence annotation: BBa_R1052
sequence: https://synbiohub.org/public/igem/BBa_R1052_sequence/1

OR1
type: SequenceAnnotation
persistent identity: annotation2081
display id: annotation2081
version: 1
top level: cI 434
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R1052/annotation2081/range2081/1

https://synbiohub.org/public/igem/BBa_R1052/annotation2081/range2081/1
type: Range
persistent identity: range2081
display id: range2081
version: 1
direction: direction/forward
top level: cI 434
start: 30
end: 43
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2082
display id: annotation2082
version: 1
top level: cI 434
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R1052/annotation2082/range2082/1

https://synbiohub.org/public/igem/BBa_R1052/annotation2082/range2082/1
type: Range
persistent identity: range2082
display id: range2082
version: 1
direction: direction/forward
top level: cI 434
start: 1
end: 6
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2084
display id: annotation2084
version: 1
top level: cI 434
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_R1052/annotation2084/range2084/1

https://synbiohub.org/public/igem/BBa_R1052/annotation2084/range2084/1
type: Range
persistent identity: range2084
display id: range2084
version: 1
direction: direction/forward
top level: cI 434
start: 35
end: 37
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2083
display id: annotation2083
version: 1
top level: cI 434
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R1052/annotation2083/range2083/1

https://synbiohub.org/public/igem/BBa_R1052/annotation2083/range2083/1
type: Range
persistent identity: range2083
display id: range2083
version: 1
direction: direction/forward
top level: cI 434
start: 2
end: 7
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2079
display id: annotation2079
version: 1
top level: cI 434
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R1052/annotation2079/range2079/1

https://synbiohub.org/public/igem/BBa_R1052/annotation2079/range2079/1
type: Range
persistent identity: range2079
display id: range2079
version: 1
direction: direction/forward
top level: cI 434
start: 1
end: 43
orientation: inline

OR2
type: SequenceAnnotation
persistent identity: annotation2080
display id: annotation2080
version: 1
top level: cI 434
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R1052/annotation2080/range2080/1

https://synbiohub.org/public/igem/BBa_R1052/annotation2080/range2080/1
type: Range
persistent identity: range2080
display id: range2080
version: 1
direction: direction/forward
top level: cI 434
start: 8
end: 21
orientation: inline

BBa_R1052
type: SequenceAnnotation
persistent identity: annotation7075
display id: annotation7075
version: 1
top level: cI 434
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_R1052/annotation7075/range7075/1

https://synbiohub.org/public/igem/BBa_R1052/annotation7075/range7075/1
type: Range
persistent identity: range7075
display id: range7075
version: 1
direction: direction/forward
top level: cI 434
start: 1
end: 46
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2087
display id: annotation2087
version: 1
top level: cI 434
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R1052/annotation2087/range2087/1

https://synbiohub.org/public/igem/BBa_R1052/annotation2087/range2087/1
type: Range
persistent identity: range2087
display id: range2087
version: 1
direction: direction/forward
top level: cI 434
start: 24
end: 29
orientation: inline

BBa_J23109
type: ComponentDefinition
persistent identity: BBa J23109
display id: BBa_J23109
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: constitutive promoter family member
creator: John Anderson
created: 2006-08-16T11:00:00Z
modified: 2015-08-31T04:08:40Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: true
mutable description: Later
mutable notes: N/A
mutable provenance: Later
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J23109_sequence/1

BBa_K165015
type: ComponentDefinition
persistent identity: BBa K165015
display id: BBa_K165015
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pADH1 yeast constituative promoter
creator: John Szymanski
created: 2008-10-25T11:00:00Z
modified: 2015-05-08T01:10:55Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _267_
m user id: 0
owner id: 2512
owning group id: 58
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This is a low-level constitutive yeast promoter.
mutable notes: -
mutable provenance: From David Drubin in the Silver Lab at Harvard.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K165015_sequence/1

PgpdA
type: ComponentDefinition
persistent identity: BBa K678024
display id: BBa_K678024
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PgpdA, promoter for Aspergillus nidulans
creator: DTU-Denmark-2
created: 2011-09-03T11:00:00Z
modified: 2015-05-08T01:13:04Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _882_
m user id: 0
owner id: 8341
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Strong constitutive Aspergillus nidulans promoter
mutable notes: Compatible with the Plug'n'Play assembly standard
mutable provenance: From the plasmid p71 from the plasmid collection of the Center for Microbial Biotechnology at the Technical University of Denmark
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: PgpdA
sequence annotation: Linker 1
sequence: https://synbiohub.org/public/igem/BBa_K678024_sequence/1

PgpdA
type: SequenceAnnotation
persistent identity: annotation2127363
display id: annotation2127363
version: 1
top level: PgpdA
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K678024/annotation2127363/range2127363/1

https://synbiohub.org/public/igem/BBa_K678024/annotation2127363/range2127363/1
type: Range
persistent identity: range2127363
display id: range2127363
version: 1
direction: direction/forward
top level: PgpdA
start: 9
end: 2312
orientation: inline

Linker 1
type: SequenceAnnotation
persistent identity: annotation2127362
display id: annotation2127362
version: 1
top level: PgpdA
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K678024/annotation2127362/range2127362/1

https://synbiohub.org/public/igem/BBa_K678024/annotation2127362/range2127362/1
type: Range
persistent identity: range2127362
display id: range2127362
version: 1
direction: direction/forward
top level: PgpdA
start: 1
end: 8
orientation: inline

BBa_K1486054
type: ComponentDefinition
persistent identity: BBa K1486054
display id: BBa_K1486054
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CheY/CheZ + split rLuc
creator: EPFL iGem team 2014
created: 2014-10-06T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: to edit
mutable notes: to edit
mutable provenance: to edit
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_K569017
component: BBa_B0034
component: BBa_K1486020
component: BBa_K1486053
component: BBa_K1486019
component: BBa_I0500
component: BBa_K629003
sequence annotation: BBa_K1486053
sequence annotation: BBa_K1486020
sequence annotation: BBa_B0034
sequence annotation: BBa_I0500
sequence annotation: BBa_K1486019
sequence annotation: BBa_K629003
sequence annotation: BBa_K569017
sequence: https://synbiohub.org/public/igem/BBa_K1486054_sequence/1

BBa_K569017
type: Component
persistent identity: component2399862
display id: component2399862
version: 1
top level: BBa_K1486054
access: public
definition: https://synbiohub.org/public/igem/BBa_K569017/1

BBa_B0034
type: Component
persistent identity: component2399866
display id: component2399866
version: 1
top level: BBa_K1486054
access: public
definition: BBa_B0034

BBa_K1486020
type: Component
persistent identity: component2399875
display id: component2399875
version: 1
top level: BBa_K1486054
access: public
definition: BBa_K1486020

BBa_K1486053
type: Component
persistent identity: component2399863
display id: component2399863
version: 1
top level: BBa_K1486054
access: public
definition: BBa_K1486053

BBa_B0034
type: Component
persistent identity: component2399861
display id: component2399861
version: 1
top level: BBa_K1486054
access: public
definition: BBa_B0034

BBa_K1486019
type: Component
persistent identity: component2399864
display id: component2399864
version: 1
top level: BBa_K1486054
access: public
definition: BBa_K1486019

BBa_I0500
type: Component
persistent identity: component2399859
display id: component2399859
version: 1
top level: BBa_K1486054
access: public
definition: pBad/araC

BBa_K629003
type: Component
persistent identity: component2399873
display id: component2399873
version: 1
top level: BBa_K1486054
access: public
definition: https://synbiohub.org/public/igem/BBa_K629003/1

BBa_K1486053
type: Component
persistent identity: component2399874
display id: component2399874
version: 1
top level: BBa_K1486054
access: public
definition: BBa_K1486053

BBa_K1486053
type: SequenceAnnotation
persistent identity: annotation2399874
display id: annotation2399874
version: 1
top level: BBa_K1486054
location: https://synbiohub.org/public/igem/BBa_K1486054/annotation2399874/range2399874/1
component: BBa_K1486053

https://synbiohub.org/public/igem/BBa_K1486054/annotation2399874/range2399874/1
type: Range
persistent identity: range2399874
display id: range2399874
version: 1
direction: direction/reverse
top level: BBa_K1486054
start: 2658
end: 2687
orientation: inline

BBa_K1486020
type: SequenceAnnotation
persistent identity: annotation2399875
display id: annotation2399875
version: 1
top level: BBa_K1486054
location: https://synbiohub.org/public/igem/BBa_K1486054/annotation2399875/range2399875/1
component: BBa_K1486020

https://synbiohub.org/public/igem/BBa_K1486054/annotation2399875/range2399875/1
type: Range
persistent identity: range2399875
display id: range2399875
version: 1
direction: direction/reverse
top level: BBa_K1486054
start: 2688
end: 3293
orientation: inline

BBa_K1486053
type: SequenceAnnotation
persistent identity: annotation2399863
display id: annotation2399863
version: 1
top level: BBa_K1486054
location: https://synbiohub.org/public/igem/BBa_K1486054/annotation2399863/range2399863/1
component: BBa_K1486053

https://synbiohub.org/public/igem/BBa_K1486054/annotation2399863/range2399863/1
type: Range
persistent identity: range2399863
display id: range2399863
version: 1
direction: direction/reverse
top level: BBa_K1486054
start: 1641
end: 1670
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2399866
display id: annotation2399866
version: 1
top level: BBa_K1486054
location: https://synbiohub.org/public/igem/BBa_K1486054/annotation2399866/range2399866/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1486054/annotation2399866/range2399866/1
type: Range
persistent identity: range2399866
display id: range2399866
version: 1
direction: direction/reverse
top level: BBa_K1486054
start: 2001
end: 2012
orientation: inline

BBa_I0500
type: SequenceAnnotation
persistent identity: annotation2399859
display id: annotation2399859
version: 1
top level: BBa_K1486054
location: https://synbiohub.org/public/igem/BBa_K1486054/annotation2399859/range2399859/1
component: BBa_I0500

https://synbiohub.org/public/igem/BBa_K1486054/annotation2399859/range2399859/1
type: Range
persistent identity: range2399859
display id: range2399859
version: 1
direction: direction/reverse
top level: BBa_K1486054
start: 1
end: 1210
orientation: inline

BBa_K1486019
type: SequenceAnnotation
persistent identity: annotation2399864
display id: annotation2399864
version: 1
top level: BBa_K1486054
location: https://synbiohub.org/public/igem/BBa_K1486054/annotation2399864/range2399864/1
component: BBa_K1486019

https://synbiohub.org/public/igem/BBa_K1486054/annotation2399864/range2399864/1
type: Range
persistent identity: range2399864
display id: range2399864
version: 1
direction: direction/reverse
top level: BBa_K1486054
start: 1671
end: 2000
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2399861
display id: annotation2399861
version: 1
top level: BBa_K1486054
location: https://synbiohub.org/public/igem/BBa_K1486054/annotation2399861/range2399861/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1486054/annotation2399861/range2399861/1
type: Range
persistent identity: range2399861
display id: range2399861
version: 1
direction: direction/reverse
top level: BBa_K1486054
start: 1211
end: 1222
orientation: inline

BBa_K629003
type: SequenceAnnotation
persistent identity: annotation2399873
display id: annotation2399873
version: 1
top level: BBa_K1486054
location: https://synbiohub.org/public/igem/BBa_K1486054/annotation2399873/range2399873/1
component: BBa_K629003

https://synbiohub.org/public/igem/BBa_K1486054/annotation2399873/range2399873/1
type: Range
persistent identity: range2399873
display id: range2399873
version: 1
direction: direction/reverse
top level: BBa_K1486054
start: 2013
end: 2657
orientation: inline

BBa_K569017
type: SequenceAnnotation
persistent identity: annotation2399862
display id: annotation2399862
version: 1
top level: BBa_K1486054
location: https://synbiohub.org/public/igem/BBa_K1486054/annotation2399862/range2399862/1
component: BBa_K569017

https://synbiohub.org/public/igem/BBa_K1486054/annotation2399862/range2399862/1
type: Range
persistent identity: range2399862
display id: range2399862
version: 1
direction: direction/reverse
top level: BBa_K1486054
start: 1223
end: 1640
orientation: inline

BBa_J64031
type: ComponentDefinition
persistent identity: BBa J64031
display id: BBa_J64031
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: sptP RBS
creator: Dan Widmaier
created: 2008-01-16T12:00:00Z
modified: 2015-08-31T01:56:26Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _98_
m user id: 0
owner id: 2407
owning group id: 98
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is the natural RBS for the sptP gene in Salmonella Type III Secretion
mutable notes: This part was designed without consideration for biobrick standard assembly. This RBS has not been explicitly tested for expression rate.
mutable provenance: Salmonella LT2 Genomic DNA
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: sptP RBS
sequence: https://synbiohub.org/public/igem/BBa_J64031_sequence/1

sptP RBS
type: SequenceAnnotation
persistent identity: annotation1959635
display id: annotation1959635
version: 1
top level: BBa_J64031
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_J64031/annotation1959635/range1959635/1

https://synbiohub.org/public/igem/BBa_J64031/annotation1959635/range1959635/1
type: Range
persistent identity: range1959635
display id: range1959635
version: 1
direction: direction/forward
top level: BBa_J64031
start: 1
end: 20
orientation: inline

PnisA
type: ComponentDefinition
persistent identity: BBa K1830001
display id: BBa_K1830001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PnisA
creator: Mathieu Husser
created: 2015-08-05T11:00:00Z
modified: 2015-08-07T11:50:06Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2256_
m user id: 27439
owner id: 27439
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Promoter and ribosome binding site of the Lactococcal nisin inducible operon.
mutable notes: Small part
mutable provenance: L. Lactis NisA operon
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: PnisA
sequence annotation: -35
sequence annotation: -10
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_K1830001_sequence/1

PnisA
type: SequenceAnnotation
persistent identity: annotation2434537
display id: annotation2434537
version: 1
top level: PnisA
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1830001/annotation2434537/range2434537/1

https://synbiohub.org/public/igem/BBa_K1830001/annotation2434537/range2434537/1
type: Range
persistent identity: range2434537
display id: range2434537
version: 1
direction: direction/forward
top level: PnisA
start: 1
end: 173
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2434539
display id: annotation2434539
version: 1
top level: PnisA
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K1830001/annotation2434539/range2434539/1

https://synbiohub.org/public/igem/BBa_K1830001/annotation2434539/range2434539/1
type: Range
persistent identity: range2434539
display id: range2434539
version: 1
direction: direction/forward
top level: PnisA
start: 97
end: 102
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2434540
display id: annotation2434540
version: 1
top level: PnisA
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K1830001/annotation2434540/range2434540/1

https://synbiohub.org/public/igem/BBa_K1830001/annotation2434540/range2434540/1
type: Range
persistent identity: range2434540
display id: range2434540
version: 1
direction: direction/forward
top level: PnisA
start: 120
end: 125
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2434538
display id: annotation2434538
version: 1
top level: PnisA
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1830001/annotation2434538/range2434538/1

https://synbiohub.org/public/igem/BBa_K1830001/annotation2434538/range2434538/1
type: Range
persistent identity: range2434538
display id: range2434538
version: 1
direction: direction/forward
top level: PnisA
start: 159
end: 165
orientation: inline

BBa_K165016
type: ComponentDefinition
persistent identity: BBa K165016
display id: BBa_K165016
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: mCYC1 minimal yeast promoter
creator: John Szymanski
created: 2008-10-25T11:00:00Z
modified: 2015-05-08T01:10:55Z
discontinued: false
dominant: false
group u list: _267_
m user id: 0
owner id: 2512
owning group id: 58
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This minimal promoter can be used as a base for designing promoters by adding binding sites upstream.
mutable notes: -
mutable provenance: From David Drubin in the Silver Lab at Harvard
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K165016_sequence/1

U061O11+D0
type: ComponentDefinition
persistent identity: BBa I732404
display id: BBa_I732404
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Family Member (U061O11+D062NUL)
creator: Zhan Jian
created: 2007-09-19T11:00:00Z
modified: 2015-08-31T04:07:58Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1572
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: A Double Reporter (LacZ-alpha and GFP-AAV) loaded on pSB3K5. For more information please refer to BBa_I732401.
mutable notes: Might be later edited.
mutable provenance: Might be later edited.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: BBa_B0010
sequence annotation: BamHI
sequence annotation: START
sequence annotation: stem_loop
sequence annotation: conserved
sequence annotation: BBa_I732006
sequence annotation: polya
sequence annotation: BBa_E0044
sequence annotation: BBa_B0034
sequence annotation: BglII
sequence annotation: lacZ
sequence annotation: STOP
sequence annotation: stop
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence: https://synbiohub.org/public/igem/BBa_I732404_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1947695
display id: annotation1947695
version: 1
top level: U061O11+D0
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_I732404/annotation1947695/range1947695/1

https://synbiohub.org/public/igem/BBa_I732404/annotation1947695/range1947695/1
type: Range
persistent identity: range1947695
display id: range1947695
version: 1
direction: direction/forward
top level: U061O11+D0
start: 1073
end: 1152
orientation: inline

BamHI
type: SequenceAnnotation
persistent identity: annotation1947685
display id: annotation1947685
version: 1
top level: U061O11+D0
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I732404/annotation1947685/range1947685/1

https://synbiohub.org/public/igem/BBa_I732404/annotation1947685/range1947685/1
type: Range
persistent identity: range1947685
display id: range1947685
version: 1
direction: direction/forward
top level: U061O11+D0
start: 12
end: 17
orientation: inline

START
type: SequenceAnnotation
persistent identity: annotation1947690
display id: annotation1947690
version: 1
top level: U061O11+D0
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_I732404/annotation1947690/range1947690/1

https://synbiohub.org/public/igem/BBa_I732404/annotation1947690/range1947690/1
type: Range
persistent identity: range1947690
display id: range1947690
version: 1
direction: direction/forward
top level: U061O11+D0
start: 46
end: 48
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation1947696
display id: annotation1947696
version: 1
top level: U061O11+D0
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_I732404/annotation1947696/range1947696/1

https://synbiohub.org/public/igem/BBa_I732404/annotation1947696/range1947696/1
type: Range
persistent identity: range1947696
display id: range1947696
version: 1
direction: direction/forward
top level: U061O11+D0
start: 1084
end: 1127
orientation: inline

conserved
type: SequenceAnnotation
persistent identity: annotation1947693
display id: annotation1947693
version: 1
top level: U061O11+D0
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_I732404/annotation1947693/range1947693/1

https://synbiohub.org/public/igem/BBa_I732404/annotation1947693/range1947693/1
type: Range
persistent identity: range1947693
display id: range1947693
version: 1
direction: direction/forward
top level: U061O11+D0
start: 292
end: 295
orientation: inline

BBa_I732006
type: SequenceAnnotation
persistent identity: annotation1947688
display id: annotation1947688
version: 1
top level: U061O11+D0
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_I732404/annotation1947688/range1947688/1

https://synbiohub.org/public/igem/BBa_I732404/annotation1947688/range1947688/1
type: Range
persistent identity: range1947688
display id: range1947688
version: 1
direction: direction/reverse
top level: U061O11+D0
start: 46
end: 279
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1947699
display id: annotation1947699
version: 1
top level: U061O11+D0
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_I732404/annotation1947699/range1947699/1

https://synbiohub.org/public/igem/BBa_I732404/annotation1947699/range1947699/1
type: Range
persistent identity: range1947699
display id: range1947699
version: 1
direction: direction/forward
top level: U061O11+D0
start: 1188
end: 1201
orientation: inline

BBa_E0044
type: SequenceAnnotation
persistent identity: annotation1947694
display id: annotation1947694
version: 1
top level: U061O11+D0
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_I732404/annotation1947694/range1947694/1

https://synbiohub.org/public/igem/BBa_I732404/annotation1947694/range1947694/1
type: Range
persistent identity: range1947694
display id: range1947694
version: 1
direction: direction/reverse
top level: U061O11+D0
start: 306
end: 1064
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1947686
display id: annotation1947686
version: 1
top level: U061O11+D0
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_I732404/annotation1947686/range1947686/1

https://synbiohub.org/public/igem/BBa_I732404/annotation1947686/range1947686/1
type: Range
persistent identity: range1947686
display id: range1947686
version: 1
direction: direction/reverse
top level: U061O11+D0
start: 28
end: 39
orientation: inline

BglII
type: SequenceAnnotation
persistent identity: annotation1947684
display id: annotation1947684
version: 1
top level: U061O11+D0
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I732404/annotation1947684/range1947684/1

https://synbiohub.org/public/igem/BBa_I732404/annotation1947684/range1947684/1
type: Range
persistent identity: range1947684
display id: range1947684
version: 1
direction: direction/forward
top level: U061O11+D0
start: 3
end: 8
orientation: inline

lacZ
type: SequenceAnnotation
persistent identity: annotation1947689
display id: annotation1947689
version: 1
top level: U061O11+D0
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_I732404/annotation1947689/range1947689/1

https://synbiohub.org/public/igem/BBa_I732404/annotation1947689/range1947689/1
type: Range
persistent identity: range1947689
display id: range1947689
version: 1
direction: direction/forward
top level: U061O11+D0
start: 46
end: 279
orientation: inline

conserved
type: SequenceAnnotation
persistent identity: annotation1947687
display id: annotation1947687
version: 1
top level: U061O11+D0
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_I732404/annotation1947687/range1947687/1

https://synbiohub.org/public/igem/BBa_I732404/annotation1947687/range1947687/1
type: Range
persistent identity: range1947687
display id: range1947687
version: 1
direction: direction/forward
top level: U061O11+D0
start: 32
end: 35
orientation: inline

STOP
type: SequenceAnnotation
persistent identity: annotation1947691
display id: annotation1947691
version: 1
top level: U061O11+D0
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_I732404/annotation1947691/range1947691/1

https://synbiohub.org/public/igem/BBa_I732404/annotation1947691/range1947691/1
type: Range
persistent identity: range1947691
display id: range1947691
version: 1
direction: direction/forward
top level: U061O11+D0
start: 274
end: 279
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1947700
display id: annotation1947700
version: 1
top level: U061O11+D0
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_I732404/annotation1947700/range1947700/1

https://synbiohub.org/public/igem/BBa_I732404/annotation1947700/range1947700/1
type: Range
persistent identity: range1947700
display id: range1947700
version: 1
direction: direction/forward
top level: U061O11+D0
start: 1194
end: 1194
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1947692
display id: annotation1947692
version: 1
top level: U061O11+D0
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_I732404/annotation1947692/range1947692/1

https://synbiohub.org/public/igem/BBa_I732404/annotation1947692/range1947692/1
type: Range
persistent identity: range1947692
display id: range1947692
version: 1
direction: direction/reverse
top level: U061O11+D0
start: 288
end: 299
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1947697
display id: annotation1947697
version: 1
top level: U061O11+D0
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_I732404/annotation1947697/range1947697/1

https://synbiohub.org/public/igem/BBa_I732404/annotation1947697/range1947697/1
type: Range
persistent identity: range1947697
display id: range1947697
version: 1
direction: direction/forward
top level: U061O11+D0
start: 1161
end: 1201
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1947698
display id: annotation1947698
version: 1
top level: U061O11+D0
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_I732404/annotation1947698/range1947698/1

https://synbiohub.org/public/igem/BBa_I732404/annotation1947698/range1947698/1
type: Range
persistent identity: range1947698
display id: range1947698
version: 1
direction: direction/forward
top level: U061O11+D0
start: 1168
end: 1187
orientation: inline

BBa_J23102
type: ComponentDefinition
persistent identity: BBa J23102
display id: BBa_J23102
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: constitutive promoter family member
creator: John Anderson
created: 2006-08-03T11:00:00Z
modified: 2015-08-31T04:08:40Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: replace later
mutable notes: N/A
mutable provenance: isolated from library of promoters
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J23102_sequence/1

BBa_J64000
type: ComponentDefinition
persistent identity: BBa J64000
display id: BBa_J64000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: rhlI promoter
creator: Jeffrey J Tabor
created: 2007-01-15T12:00:00Z
modified: 2015-08-31T01:56:26Z
discontinued: false
dominant: false
experience: experience/None
group u list: _98_
m user id: 0
owner id: 88
owning group id: 98
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Promoter driving the rhlI gene in Pseudomonas aeruginosa. Activated by the RhlR+C4HSL. Also likely activated by LasR+3OC12HSL
mutable notes: none
mutable provenance: Genomic sequence of Pseudomonas aeruginosa
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: Rhl box
sequence: https://synbiohub.org/public/igem/BBa_J64000_sequence/1

Rhl box
type: SequenceAnnotation
persistent identity: annotation1910614
display id: annotation1910614
version: 1
top level: BBa_J64000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J64000/annotation1910614/range1910614/1

https://synbiohub.org/public/igem/BBa_J64000/annotation1910614/range1910614/1
type: Range
persistent identity: range1910614
display id: range1910614
version: 1
direction: direction/forward
top level: BBa_J64000
start: 11
end: 30
orientation: inline

BBa_J61144
type: ComponentDefinition
persistent identity: BBa J61144
display id: BBa_J61144
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: {rbs1} Library Member in BBb
creator: John Anderson
created: 2007-09-03T11:00:00Z
modified: 2015-08-31T01:56:25Z
discontinued: false
dominant: false
experience: experience/None
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: {{BBbRBS1Family}}
mutable notes: {{BerkiGEM2007-Biobrick2.0Intro}}
mutable provenance: Synthetic
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J61144_sequence/1

BBa_I746365
type: ComponentDefinition
persistent identity: BBa I746365
display id: BBa_I746365
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PLL promoter from P4 phage
creator: Stefan Milde
created: 2007-09-10T11:00:00Z
modified: 2015-08-31T04:08:04Z
discontinued: false
dominant: false
group u list: _116_
m user id: 0
owner id: 2122
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This is the PLL promoter taken from the P4 phage genome. It is an inducible promoter that is activated by a class of activators, including P2 ogr (I746350), PSP3 pag (I746351) and phiR73 delta (I746352). These different activators should cause different levels of activity of the PLL promoter.
mutable notes: no special considerations
mutable provenance: P4 phage genome sequence
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: PLL
sequence: https://synbiohub.org/public/igem/BBa_I746365_sequence/1

PLL
type: SequenceAnnotation
persistent identity: annotation1943788
display id: annotation1943788
version: 1
top level: BBa_I746365
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I746365/annotation1943788/range1943788/1

https://synbiohub.org/public/igem/BBa_I746365/annotation1943788/range1943788/1
type: Range
persistent identity: range1943788
display id: range1943788
version: 1
direction: direction/forward
top level: BBa_I746365
start: 1
end: 92
orientation: inline

U061O11+D0
type: ComponentDefinition
persistent identity: BBa I732436
display id: BBa_I732436
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Family Member (U061O11+D062O22)
creator: Zhan Jian
created: 2007-10-13T11:00:00Z
modified: 2015-08-31T04:07:58Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732436_sequence/1

BBa_J64015
type: ComponentDefinition
persistent identity: BBa J64015
display id: BBa_J64015
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: sopA RBS
creator: Dan Widmaier
created: 2008-01-16T12:00:00Z
modified: 2015-08-31T01:56:26Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _98_
m user id: 0
owner id: 2407
owning group id: 98
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is the natural RBS associated with the sopA effector protein in the Salmonella SPI-1 system.
mutable notes: This part was not designed with compliance to biobrick standard assembly in mind. This RBS hasn't been explicitly tested in an expression assay such as GFP fluorescence or B-Gal expression.
mutable provenance: Salmonella LT2 Genomic DNA
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: sopA RBS
sequence: https://synbiohub.org/public/igem/BBa_J64015_sequence/1

sopA RBS
type: SequenceAnnotation
persistent identity: annotation1959614
display id: annotation1959614
version: 1
top level: BBa_J64015
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_J64015/annotation1959614/range1959614/1

https://synbiohub.org/public/igem/BBa_J64015/annotation1959614/range1959614/1
type: Range
persistent identity: range1959614
display id: range1959614
version: 1
direction: direction/forward
top level: BBa_J64015
start: 1
end: 16
orientation: inline

BBa_I11010
type: ComponentDefinition
persistent identity: BBa I11010
display id: BBa_I11010
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS
creator: bu
created: 2004-09-02T11:00:00Z
modified: 2015-08-31T04:07:30Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2_
m user id: 0
owner id: 102
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: ribosome binding site
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_I11010_sequence/1

RBS
type: SequenceAnnotation
persistent identity: annotation1147662
display id: annotation1147662
version: 1
top level: BBa_I11010
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_I11010/annotation1147662/range1147662/1

https://synbiohub.org/public/igem/BBa_I11010/annotation1147662/range1147662/1
type: Range
persistent identity: range1147662
display id: range1147662
version: 1
direction: direction/forward
top level: BBa_I11010
start: 1
end: 6
orientation: inline

BBa_K292000
type: ComponentDefinition
persistent identity: BBa K292000
display id: BBa_K292000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Double terminator + constitutive promoter
creator: David Charoy
created: 2009-10-17T11:00:00Z
modified: 2015-05-08T01:11:49Z
discontinued: false
dominant: true
group u list: _394_
m user id: 0
owner id: 5212
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part contains a double terminator and a constitutive prokaryotic promoter. It is a simple promoter to begin gene transcription. For Sup???biotech-Paris (2009) team this part is used after to be added to a recombinant lambda phage to begin the transcription of synthetic genes inside the Lambda phage genome.
mutable notes: N/A
mutable provenance: BBa_B0014 : Designed by Reshma Shetty Group: Registry (2003-07-16) BBa_J23100 : Designed by John Anderson Group: iGEM2006_Berkeley (2006-08-04)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_J23100
component: BBa_B0012
component: BBa_B0011
sequence annotation: BBa_B0011
sequence annotation: BBa_J23100
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_K292000_sequence/1

BBa_J23100
type: Component
persistent identity: component2059163
display id: component2059163
version: 1
top level: BBa_K292000
access: public
definition: BBa_J23100

BBa_B0012
type: Component
persistent identity: component2059157
display id: component2059157
version: 1
top level: BBa_K292000
access: public
definition: BBa_B0012

BBa_B0011
type: Component
persistent identity: component2059161
display id: component2059161
version: 1
top level: BBa_K292000
access: public
definition: https://synbiohub.org/public/igem/BBa_B0011/1

BBa_B0011
type: SequenceAnnotation
persistent identity: annotation2059161
display id: annotation2059161
version: 1
top level: BBa_K292000
location: https://synbiohub.org/public/igem/BBa_K292000/annotation2059161/range2059161/1
component: BBa_B0011

https://synbiohub.org/public/igem/BBa_K292000/annotation2059161/range2059161/1
type: Range
persistent identity: range2059161
display id: range2059161
version: 1
direction: direction/forward
top level: BBa_K292000
start: 50
end: 95
orientation: inline

BBa_J23100
type: SequenceAnnotation
persistent identity: annotation2059163
display id: annotation2059163
version: 1
top level: BBa_K292000
location: https://synbiohub.org/public/igem/BBa_K292000/annotation2059163/range2059163/1
component: BBa_J23100

https://synbiohub.org/public/igem/BBa_K292000/annotation2059163/range2059163/1
type: Range
persistent identity: range2059163
display id: range2059163
version: 1
direction: direction/reverse
top level: BBa_K292000
start: 104
end: 138
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2059157
display id: annotation2059157
version: 1
top level: BBa_K292000
location: https://synbiohub.org/public/igem/BBa_K292000/annotation2059157/range2059157/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K292000/annotation2059157/range2059157/1
type: Range
persistent identity: range2059157
display id: range2059157
version: 1
direction: direction/forward
top level: BBa_K292000
start: 1
end: 41
orientation: inline

BBa_B0071
type: ComponentDefinition
persistent identity: BBa B0071
display id: BBa_B0071
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Specialized RBS
creator: Barry Canton
created: 2006-03-03T12:00:00Z
modified: 2015-08-31T04:07:21Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_6_
m user id: 0
owner id: 135
owning group id: 6
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This RBS has the modified Shine-Dalgarno sequence, it has a reduced secondary structure relative to using the actual Brink sequence with mixed connective sites flanking it. This RBS is modified from B0071 by reducing the 3' sequence so that the SD to ATG spacing is closer to that which is optimal for E. coli.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_B0071_sequence/1

BBa_J64712
type: ComponentDefinition
persistent identity: BBa J64712
display id: BBa_J64712
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LasR/LasI Inducible & RHLR/RHLI repressible Promoter
creator: laura lavery
created: 2007-03-24T12:00:00Z
modified: 2015-05-08T01:08:17Z
discontinued: false
dominant: false
experience: experience/None
group u list: _98_
m user id: 0
owner id: 1431
owning group id: 98
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This part is similar to BBa_R0079 but has an added binding site for RHLR/RHLI in between the -35 and -10 region. Thus this promoter is induced by LasR/LasI but repressed by RHLR/RHLI binding.
mutable notes: The natural spacer between the -35 and -10 is 14bp and the natural O1 site for RHLR/RHLI is 20bp long. There was not much over lap of the -35 or the -10 with this sequence, so it was nessicary to chop off some of the binding site sequence in order to maintain the correct spacing and preserve the -35 and -10 sequence. The binding site sequence is missing a tcc on is 5' end and a t on its 3' end.
mutable provenance: Rust L. et. al., "Analysis of the Pseudomonas aeruginosa Elastase (lasB) Regulatory Region". Journal of Bacteriology, 1996.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -10
sequence annotation: -35
sequence annotation: LasR/LasI O1
sequence annotation: LasR/LasI O2
sequence annotation: RHLR/RHLI
sequence: https://synbiohub.org/public/igem/BBa_J64712_sequence/1

-10
type: SequenceAnnotation
persistent identity: annotation1921068
display id: annotation1921068
version: 1
top level: BBa_J64712
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J64712/annotation1921068/range1921068/1

https://synbiohub.org/public/igem/BBa_J64712/annotation1921068/range1921068/1
type: Range
persistent identity: range1921068
display id: range1921068
version: 1
direction: direction/forward
top level: BBa_J64712
start: 140
end: 145
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1921067
display id: annotation1921067
version: 1
top level: BBa_J64712
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J64712/annotation1921067/range1921067/1

https://synbiohub.org/public/igem/BBa_J64712/annotation1921067/range1921067/1
type: Range
persistent identity: range1921067
display id: range1921067
version: 1
direction: direction/forward
top level: BBa_J64712
start: 117
end: 122
orientation: inline

LasR/LasI O1
type: SequenceAnnotation
persistent identity: annotation1921065
display id: annotation1921065
version: 1
top level: BBa_J64712
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_J64712/annotation1921065/range1921065/1

https://synbiohub.org/public/igem/BBa_J64712/annotation1921065/range1921065/1
type: Range
persistent identity: range1921065
display id: range1921065
version: 1
direction: direction/forward
top level: BBa_J64712
start: 106
end: 125
orientation: inline

LasR/LasI O2
type: SequenceAnnotation
persistent identity: annotation1921064
display id: annotation1921064
version: 1
top level: BBa_J64712
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_J64712/annotation1921064/range1921064/1

https://synbiohub.org/public/igem/BBa_J64712/annotation1921064/range1921064/1
type: Range
persistent identity: range1921064
display id: range1921064
version: 1
direction: direction/forward
top level: BBa_J64712
start: 46
end: 64
orientation: inline

RHLR/RHLI
type: SequenceAnnotation
persistent identity: annotation1921066
display id: annotation1921066
version: 1
top level: BBa_J64712
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_J64712/annotation1921066/range1921066/1

https://synbiohub.org/public/igem/BBa_J64712/annotation1921066/range1921066/1
type: Range
persistent identity: range1921066
display id: range1921066
version: 1
direction: direction/forward
top level: BBa_J64712
start: 125
end: 141
orientation: inline

BBa_K1585116
type: ComponentDefinition
persistent identity: BBa K1585116
display id: BBa_K1585116
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Anderson Promoter with lacI binding site
creator: Tobias Schwanemann
created: 2015-08-31T11:00:00Z
modified: 2015-09-01T02:19:47Z
discontinued: false
dominant: false
experience: experience/Fails
group u list: _2002_
m user id: 24474
owner id: 24474
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Promoters of the Anderson Promoter Collection with lacI binding site derived from [http://parts.igem.org/Part:BBa_R0010 R_0010] to make them [http://openwetware.org/wiki/IPTG IPTG] iducible.
mutable notes: We choosed lacI-binding-site of part BBa_R0010 because we assumed older parts would be more reliable. The part is shipped in backbone [http://parts.igem.org/Part:BBa_J61002 J61002] because the functionality is directly visible by adding IPTG. RFP ([http://parts.igem.org/Part:BBa_E1010 E1010]) should get expressed then.
mutable provenance: Assambly of [http://parts.igem.org/Part:BBa_J23103 J23103] and lacI-binding-site of [http://parts.igem.org/Part:BBa_R0010 R_0010] The "lacI binding site" of Part [http://parts.igem.org/Part:BBa_R0010 R_0010] differs from lacO from Part [http://parts.igem.org/Part:BBa_J33207 BBa_J33207].
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
component: BBa_K1585999
component: BBa_J23116
sequence annotation: BBa_J23116
sequence annotation: BBa_K1585999
sequence: https://synbiohub.org/public/igem/BBa_K1585116_sequence/1

BBa_K1585999
type: Component
persistent identity: component2441710
display id: component2441710
version: 1
top level: BBa_K1585116
access: public
definition: https://synbiohub.org/public/igem/BBa_K1585999/1

BBa_J23116
type: Component
persistent identity: component2441708
display id: component2441708
version: 1
top level: BBa_K1585116
access: public
definition: BBa_J23116

BBa_J23116
type: SequenceAnnotation
persistent identity: annotation2441708
display id: annotation2441708
version: 1
top level: BBa_K1585116
location: https://synbiohub.org/public/igem/BBa_K1585116/annotation2441708/range2441708/1
component: BBa_J23116

https://synbiohub.org/public/igem/BBa_K1585116/annotation2441708/range2441708/1
type: Range
persistent identity: range2441708
display id: range2441708
version: 1
direction: direction/reverse
top level: BBa_K1585116
start: 1
end: 35
orientation: inline

BBa_K1585999
type: SequenceAnnotation
persistent identity: annotation2441710
display id: annotation2441710
version: 1
top level: BBa_K1585116
location: https://synbiohub.org/public/igem/BBa_K1585116/annotation2441710/range2441710/1
component: BBa_K1585999

https://synbiohub.org/public/igem/BBa_K1585116/annotation2441710/range2441710/1
type: Range
persistent identity: range2441710
display id: range2441710
version: 1
direction: direction/reverse
top level: BBa_K1585116
start: 44
end: 78
orientation: inline

BBa_K090501
type: ComponentDefinition
persistent identity: BBa K090501
display id: BBa_K090501
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Gram-Positive IPTG-Inducible Promoter
creator: Daniel Goodman
created: 2008-10-27T12:00:00Z
modified: 2015-05-08T01:08:37Z
discontinued: false
dominant: false
experience: experience/None
group u list: _188_
m user id: 0
owner id: 3501
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This is an IPTG-inducible promoter for use with gram-positive organisms. It requires a downstream gram-positive ribosomal binding site and coding sequence to function. We only have experience using this part in B. subtilis, but it should function in most gram-positive organisms. It has not been tested and will most likely not work in E. coli.
mutable notes: We found the functional annotation in the associated paper: Kaltwasser M., T. Wiegert, and W. Schumann. 2002. Construction and Application of Epitope- and Green Fluorescent Protein-Tagging Integration Vectors for Bacillus subtilis. Appl. Environ. Microbiol 68:2624-2628 The primers we used to extract it (sans biobrick ends) are as follow: Forward: AGAACAACCTCTGCTAAAATTC Reverse: AAGCTTAATTGTTATCCGCTC
mutable provenance: We PCRed this part out of the pMUTIN-YFP gram positive vector, acquired from the Bacillus Genetic Stock Center (bgsc.org), catalog number ECE151.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: lacOid Operator
sequence annotation: -10
sequence annotation: -35
sequence: https://synbiohub.org/public/igem/BBa_K090501_sequence/1

lacOid Operator
type: SequenceAnnotation
persistent identity: annotation1991824
display id: annotation1991824
version: 1
top level: BBa_K090501
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K090501/annotation1991824/range1991824/1

https://synbiohub.org/public/igem/BBa_K090501/annotation1991824/range1991824/1
type: Range
persistent identity: range1991824
display id: range1991824
version: 1
direction: direction/forward
top level: BBa_K090501
start: 78
end: 97
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1991823
display id: annotation1991823
version: 1
top level: BBa_K090501
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K090501/annotation1991823/range1991823/1

https://synbiohub.org/public/igem/BBa_K090501/annotation1991823/range1991823/1
type: Range
persistent identity: range1991823
display id: range1991823
version: 1
direction: direction/forward
top level: BBa_K090501
start: 66
end: 71
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1991822
display id: annotation1991822
version: 1
top level: BBa_K090501
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K090501/annotation1991822/range1991822/1

https://synbiohub.org/public/igem/BBa_K090501/annotation1991822/range1991822/1
type: Range
persistent identity: range1991822
display id: range1991822
version: 1
direction: direction/forward
top level: BBa_K090501
start: 43
end: 48
orientation: inline

BBa_K1140006
type: ComponentDefinition
persistent identity: BBa K1140006
display id: BBa_K1140006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pTet + 37 oC RNA thermometer + mCherry (LVA)
creator: Janssel Reyes del Castillo
created: 2013-09-12T11:00:00Z
modified: 2015-05-08T01:09:23Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1452_
m user id: 0
owner id: 12878
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This part contains a promoter regulated by TetR protein. The Transcription is regulated by a 37 oC riboswitch. The protein codified is a RFP with a LVA tag.
mutable notes: The prefix sites are EcoRI and XbaI and the sufix sites are SpeI and PstI.
mutable provenance: Obtained synthetically. Promoter pTet. Based on a fusion of a RBS and a Riboswitch. RFP with a LVA tag (Further information coming soon).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: t7 terminator
sequence annotation: mCherry+LVA
sequence annotation: pTet
sequence annotation: U6 RNA thermometer
sequence: https://synbiohub.org/public/igem/BBa_K1140006_sequence/1

t7 terminator
type: SequenceAnnotation
persistent identity: annotation2341072
display id: annotation2341072
version: 1
top level: BBa_K1140006
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1140006/annotation2341072/range2341072/1

https://synbiohub.org/public/igem/BBa_K1140006/annotation2341072/range2341072/1
type: Range
persistent identity: range2341072
display id: range2341072
version: 1
direction: direction/forward
top level: BBa_K1140006
start: 844
end: 887
orientation: inline

mCherry+LVA
type: SequenceAnnotation
persistent identity: annotation2341071
display id: annotation2341071
version: 1
top level: BBa_K1140006
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1140006/annotation2341071/range2341071/1

https://synbiohub.org/public/igem/BBa_K1140006/annotation2341071/range2341071/1
type: Range
persistent identity: range2341071
display id: range2341071
version: 1
direction: direction/forward
top level: BBa_K1140006
start: 122
end: 835
orientation: inline

pTet
type: SequenceAnnotation
persistent identity: annotation2341069
display id: annotation2341069
version: 1
top level: BBa_K1140006
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1140006/annotation2341069/range2341069/1

https://synbiohub.org/public/igem/BBa_K1140006/annotation2341069/range2341069/1
type: Range
persistent identity: range2341069
display id: range2341069
version: 1
direction: direction/forward
top level: BBa_K1140006
start: 1
end: 54
orientation: inline

U6 RNA thermometer
type: SequenceAnnotation
persistent identity: annotation2341070
display id: annotation2341070
version: 1
top level: BBa_K1140006
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1140006/annotation2341070/range2341070/1

https://synbiohub.org/public/igem/BBa_K1140006/annotation2341070/range2341070/1
type: Range
persistent identity: range2341070
display id: range2341070
version: 1
direction: direction/forward
top level: BBa_K1140006
start: 63
end: 121
orientation: inline

U085O11+D0
type: ComponentDefinition
persistent identity: BBa I732423
display id: BBa_I732423
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Family Member (U085O11+D050O22)
creator: Zhan Jian
created: 2007-10-13T11:00:00Z
modified: 2015-08-31T04:07:58Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732423_sequence/1

BBa_K1072004
type: ComponentDefinition
persistent identity: BBa K1072004
display id: BBa_K1072004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: alpha-ALS Coding Region
creator: Zewei yan, Yichong wang and Junjie zhang
created: 2013-09-06T11:00:00Z
modified: 2015-05-08T01:09:01Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1381_
m user id: 0
owner id: 16002
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: BBa_K1072004 is the conding region of α-acetolactate synthetase(α-ALS).
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: alpha-ALS
sequence: https://synbiohub.org/public/igem/BBa_K1072004_sequence/1

alpha-ALS
type: SequenceAnnotation
persistent identity: annotation2335232
display id: annotation2335232
version: 1
top level: BBa_K1072004
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1072004/annotation2335232/range2335232/1

https://synbiohub.org/public/igem/BBa_K1072004/annotation2335232/range2335232/1
type: Range
persistent identity: range2335232
display id: range2335232
version: 1
direction: direction/forward
top level: BBa_K1072004
start: 1
end: 1665
orientation: inline

BBa_K1585115
type: ComponentDefinition
persistent identity: BBa K1585115
display id: BBa_K1585115
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Anderson Promoter with lacI binding site
creator: Tobias Schwanemann
created: 2015-08-31T11:00:00Z
modified: 2015-09-01T02:13:08Z
discontinued: false
dominant: false
experience: experience/Fails
group u list: _2002_
m user id: 24474
owner id: 24474
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Promoters of the Anderson Promoter Collection with lacI binding site derived from [http://parts.igem.org/Part:BBa_R0010 R_0010] to make them [http://openwetware.org/wiki/IPTG IPTG] iducible.
mutable notes: We choosed lacI-binding-site of part BBa_R0010 because we assumed older parts would be more reliable. The part is shipped in backbone [http://parts.igem.org/Part:BBa_J61002 J61002] because the functionality is directly visible by adding IPTG. RFP ([http://parts.igem.org/Part:BBa_E1010 E1010]) should get expressed then.
mutable provenance: Assambly of [http://parts.igem.org/Part:BBa_J23110 J23110] and lacI-binding-site of [http://parts.igem.org/Part:BBa_R0010 R_0010] The "lacI binding site" of Part [http://parts.igem.org/Part:BBa_R0010 R_0010] differs from lacO from Part [http://parts.igem.org/Part:BBa_J33207 BBa_J33207].
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
component: BBa_K1585999
component: BBa_J23115
sequence annotation: BBa_K1585999
sequence annotation: BBa_J23115
sequence: https://synbiohub.org/public/igem/BBa_K1585115_sequence/1

BBa_K1585999
type: Component
persistent identity: component2441707
display id: component2441707
version: 1
top level: BBa_K1585115
access: public
definition: https://synbiohub.org/public/igem/BBa_K1585999/1

BBa_J23115
type: Component
persistent identity: component2441705
display id: component2441705
version: 1
top level: BBa_K1585115
access: public
definition: BBa_J23115

BBa_K1585999
type: SequenceAnnotation
persistent identity: annotation2441707
display id: annotation2441707
version: 1
top level: BBa_K1585115
location: https://synbiohub.org/public/igem/BBa_K1585115/annotation2441707/range2441707/1
component: BBa_K1585999

https://synbiohub.org/public/igem/BBa_K1585115/annotation2441707/range2441707/1
type: Range
persistent identity: range2441707
display id: range2441707
version: 1
direction: direction/reverse
top level: BBa_K1585115
start: 44
end: 78
orientation: inline

BBa_J23115
type: SequenceAnnotation
persistent identity: annotation2441705
display id: annotation2441705
version: 1
top level: BBa_K1585115
location: https://synbiohub.org/public/igem/BBa_K1585115/annotation2441705/range2441705/1
component: BBa_J23115

https://synbiohub.org/public/igem/BBa_K1585115/annotation2441705/range2441705/1
type: Range
persistent identity: range2441705
display id: range2441705
version: 1
direction: direction/reverse
top level: BBa_K1585115
start: 1
end: 35
orientation: inline

BBa_J61109
type: ComponentDefinition
persistent identity: BBa J61109
display id: BBa_J61109
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Ribosome Binding Site Family Member
creator: John Anderson
created: 2007-04-23T11:00:00Z
modified: 2015-08-31T02:03:00Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: false
mutable description: {{JCA_Arkin_RBSFamily}}
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J61109_sequence/1

BBa_K180002
type: ComponentDefinition
persistent identity: BBa K180002
display id: BBa_K180002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: tac pR testing plasmid (GFP)
creator: Richard Wilson, Stephanie Chambers
created: 2009-10-13T11:00:00Z
modified: 2015-05-08T01:11:06Z
discontinued: false
dominant: false
experience: experience/None
group u list: _278_
m user id: 0
owner id: 4082
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: PTAC promoter followed by an RBS, green fluorescent protein and stop codon, for testing the effectiveness of the PTAC hybrid promoter designed by iGEM2009 Team:Southampton.
mutable notes: Inclusion of the RBS and stop sequence to ensure correct functionality observed.
mutable provenance: K180000 and existing biobrick parts.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_E0040
component: BBa_K180000
component: BBa_B0034
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0034
sequence annotation: BBa_K180000
sequence annotation: BBa_E0040
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_K180002_sequence/1

BBa_E0040
type: Component
persistent identity: component2037771
display id: component2037771
version: 1
top level: BBa_K180002
access: public
definition: https://synbiohub.org/public/igem/BBa_E0040/1

BBa_K180000
type: Component
persistent identity: component2037767
display id: component2037767
version: 1
top level: BBa_K180002
access: public
definition: BBa_K180000

BBa_B0034
type: Component
persistent identity: component2037769
display id: component2037769
version: 1
top level: BBa_K180002
access: public
definition: BBa_B0034

BBa_B0012
type: Component
persistent identity: component2037774
display id: component2037774
version: 1
top level: BBa_K180002
access: public
definition: BBa_B0012

BBa_B0010
type: Component
persistent identity: component2037772
display id: component2037772
version: 1
top level: BBa_K180002
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2037769
display id: annotation2037769
version: 1
top level: BBa_K180002
location: https://synbiohub.org/public/igem/BBa_K180002/annotation2037769/range2037769/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K180002/annotation2037769/range2037769/1
type: Range
persistent identity: range2037769
display id: range2037769
version: 1
direction: direction/reverse
top level: BBa_K180002
start: 1456
end: 1467
orientation: inline

BBa_K180000
type: SequenceAnnotation
persistent identity: annotation2037767
display id: annotation2037767
version: 1
top level: BBa_K180002
location: https://synbiohub.org/public/igem/BBa_K180002/annotation2037767/range2037767/1
component: BBa_K180000

https://synbiohub.org/public/igem/BBa_K180002/annotation2037767/range2037767/1
type: Range
persistent identity: range2037767
display id: range2037767
version: 1
direction: direction/reverse
top level: BBa_K180002
start: 1
end: 1447
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation2037771
display id: annotation2037771
version: 1
top level: BBa_K180002
location: https://synbiohub.org/public/igem/BBa_K180002/annotation2037771/range2037771/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_K180002/annotation2037771/range2037771/1
type: Range
persistent identity: range2037771
display id: range2037771
version: 1
direction: direction/reverse
top level: BBa_K180002
start: 1474
end: 2193
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2037772
display id: annotation2037772
version: 1
top level: BBa_K180002
location: https://synbiohub.org/public/igem/BBa_K180002/annotation2037772/range2037772/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K180002/annotation2037772/range2037772/1
type: Range
persistent identity: range2037772
display id: range2037772
version: 1
direction: direction/forward
top level: BBa_K180002
start: 2202
end: 2281
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2037774
display id: annotation2037774
version: 1
top level: BBa_K180002
location: https://synbiohub.org/public/igem/BBa_K180002/annotation2037774/range2037774/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K180002/annotation2037774/range2037774/1
type: Range
persistent identity: range2037774
display id: range2037774
version: 1
direction: direction/forward
top level: BBa_K180002
start: 2290
end: 2330
orientation: inline

BBa_J16101
type: ComponentDefinition
persistent identity: BBa J16101
display id: BBa_J16101
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: BanAp - Banana-induced Promoter
creator: James Brown
created: 2006-07-23T11:00:00Z
modified: 2015-08-31T04:08:33Z
discontinued: false
dominant: false
group u list: _48_
m user id: 0
owner id: 740
owning group id: 1
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Taken from no other than Curious George, this promoter is induced by the presence of banana in the metabolism of any mammal. It is very weak however without the presence of the HngR protein. For transcription to reach significant levels both HngR and banana must be present
mutable notes: Monkeys can be dangerous when aggrevated
mutable provenance: Curious George's very own DNA
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J16101_sequence/1

eYFP-GOI
type: ComponentDefinition
persistent identity: BBa K678010
display id: BBa_K678010
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: eYFP-GOI
creator: DTU-Danmark-2
created: 2011-09-12T11:00:00Z
modified: 2015-05-08T01:13:04Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _882_
m user id: 0
owner id: 10556
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part was designed for the Plug'n'Play with DNA assembly standard (RFC 80), therefore this part contains tails at the 5' and 3' ends as shown on the figure below.
mutable notes: No
mutable provenance: The Danish Cancer Society
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: Linker 2
sequence annotation: eYFP-GOI
sequence annotation: Kozak
sequence: https://synbiohub.org/public/igem/BBa_K678010_sequence/1

Linker 2
type: SequenceAnnotation
persistent identity: annotation2127764
display id: annotation2127764
version: 1
top level: eYFP-GOI
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K678010/annotation2127764/range2127764/1

https://synbiohub.org/public/igem/BBa_K678010/annotation2127764/range2127764/1
type: Range
persistent identity: range2127764
display id: range2127764
version: 1
direction: direction/forward
top level: eYFP-GOI
start: 1
end: 8
orientation: inline

eYFP-GOI
type: SequenceAnnotation
persistent identity: annotation2127765
display id: annotation2127765
version: 1
top level: eYFP-GOI
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K678010/annotation2127765/range2127765/1

https://synbiohub.org/public/igem/BBa_K678010/annotation2127765/range2127765/1
type: Range
persistent identity: range2127765
display id: range2127765
version: 1
direction: direction/forward
top level: eYFP-GOI
start: 16
end: 810
orientation: inline

Kozak
type: SequenceAnnotation
persistent identity: annotation2127776
display id: annotation2127776
version: 1
top level: eYFP-GOI
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K678010/annotation2127776/range2127776/1

https://synbiohub.org/public/igem/BBa_K678010/annotation2127776/range2127776/1
type: Range
persistent identity: range2127776
display id: range2127776
version: 1
direction: direction/forward
top level: eYFP-GOI
start: 9
end: 19
orientation: inline

BBa_K1486019
type: ComponentDefinition
persistent identity: BBa K1486019
display id: BBa_K1486019
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: rLuc[1]
creator: EPFL iGem team 2014
created: 2014-09-14T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: his is the first part of the split Renilla luciferase. To be used with fLuc[2].
mutable notes: to edit
mutable provenance: to edit
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence: https://synbiohub.org/public/igem/BBa_K1486019_sequence/1

BBa_I742156
type: ComponentDefinition
persistent identity: BBa I742156
display id: BBa_I742156
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: crtZ native rbs
creator: Chris French
created: 2007-10-23T11:00:00Z
modified: 2015-08-31T04:08:03Z
discontinued: false
dominant: false
experience: experience/None
group u list: _123_
m user id: 0
owner id: 837
owning group id: 163
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Native ribosome binding site for crtZ (beta-carotene hydroxylase) from Pantoea ananatis (formerly Erwinia uredovora) DSMZ 30080 (ATCC 19321). Accession: D90087.
mutable notes: A virtual part to be added to the coding sequence blunt (without a scar).
mutable provenance: Pantoea ananatis (formerly Erwinia uredovora) DSMZ 30080 (ATCC 19321). Accession: D90087.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_I742156_sequence/1

BBa_K1895002
type: ComponentDefinition
persistent identity: BBa K1895002
display id: BBa_K1895002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: dnaK Promoter
creator: iGEM16_Newcastle
created: 2016-09-05T11:00:00Z
modified: 2016-09-06T04:33:29Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2361_
m user id: 29859
owner id: 29859
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This is the dnaK promoter
mutable notes: [TODO]
mutable provenance: Uniprot
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K1895002_sequence/1

hygR
type: ComponentDefinition
persistent identity: BBa K678021
display id: BBa_K678021
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Hygromycin cassette
creator: DTU-Denmark-2
created: 2011-09-03T11:00:00Z
modified: 2015-05-08T01:13:04Z
discontinued: false
dominant: false
experience: experience/None
group u list: _882_
m user id: 0
owner id: 8341
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Hygromycin cassette for selection of mammalian cells. The selection cassette contains the SV40 promoter, the hygromycin resistance gene, and the SV40 poly A as terminator
mutable notes: Compatible with the Plu'n'Play assembly standard
mutable provenance: From plasmid pcDNA3.1/Hygro from Invitrogen
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Other
role: SO:0000110
sequence annotation: Hygromycin cassette
sequence annotation: Linker 5
sequence: https://synbiohub.org/public/igem/BBa_K678021_sequence/1

Hygromycin cassette
type: SequenceAnnotation
persistent identity: annotation2127357
display id: annotation2127357
version: 1
top level: hygR
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K678021/annotation2127357/range2127357/1

https://synbiohub.org/public/igem/BBa_K678021/annotation2127357/range2127357/1
type: Range
persistent identity: range2127357
display id: range2127357
version: 1
direction: direction/forward
top level: hygR
start: 9
end: 1759
orientation: inline

Linker 5
type: SequenceAnnotation
persistent identity: annotation2127356
display id: annotation2127356
version: 1
top level: hygR
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K678021/annotation2127356/range2127356/1

https://synbiohub.org/public/igem/BBa_K678021/annotation2127356/range2127356/1
type: Range
persistent identity: range2127356
display id: range2127356
version: 1
direction: direction/forward
top level: hygR
start: 1
end: 8
orientation: inline

BBa_J15502
type: ComponentDefinition
persistent identity: BBa J15502
display id: BBa_J15502
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: copA promoter
creator: Chris French
created: 2008-05-15T11:00:00Z
modified: 2015-08-31T04:08:32Z
discontinued: false
dominant: false
group u list: _163_
m user id: 0
owner id: 837
owning group id: 163
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Promoter of the copA copper efflux gene from Escherichia coli K12. This promoter is induced in the presence of copper ions, regulated by the activator protein CueR (CopR).
mutable notes: No special considerations
mutable provenance: Escherichia coli K12
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -35
sequence annotation: CpxR
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_J15502_sequence/1

-35
type: SequenceAnnotation
persistent identity: annotation1963239
display id: annotation1963239
version: 1
top level: BBa_J15502
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_J15502/annotation1963239/range1963239/1

https://synbiohub.org/public/igem/BBa_J15502/annotation1963239/range1963239/1
type: Range
persistent identity: range1963239
display id: range1963239
version: 1
direction: direction/forward
top level: BBa_J15502
start: 248
end: 253
orientation: inline

CpxR
type: SequenceAnnotation
persistent identity: annotation1963241
display id: annotation1963241
version: 1
top level: BBa_J15502
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_J15502/annotation1963241/range1963241/1

https://synbiohub.org/public/igem/BBa_J15502/annotation1963241/range1963241/1
type: Range
persistent identity: range1963241
display id: range1963241
version: 1
direction: direction/forward
top level: BBa_J15502
start: 214
end: 227
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1963240
display id: annotation1963240
version: 1
top level: BBa_J15502
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_J15502/annotation1963240/range1963240/1

https://synbiohub.org/public/igem/BBa_J15502/annotation1963240/range1963240/1
type: Range
persistent identity: range1963240
display id: range1963240
version: 1
direction: direction/forward
top level: BBa_J15502
start: 273
end: 278
orientation: inline

U097NUL+D0
type: ComponentDefinition
persistent identity: BBa I732407
display id: BBa_I732407
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Family Member (U097NUL+D002O22)
creator: Zhan Jian
created: 2007-09-19T11:00:00Z
modified: 2015-08-31T04:07:58Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1572
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: A Double Reporter (LacZ-alpha and GFP-AAV) loaded on pSB3C5. For more information please refer to BBa_I732401.
mutable notes: Might be later edited.
mutable provenance: Might be later edited.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: stop
sequence annotation: stem_loop
sequence annotation: conserved
sequence annotation: START
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: BBa_B0034
sequence annotation: BamHI
sequence annotation: lacZ
sequence annotation: BBa_I732007
sequence annotation: BglII
sequence annotation: BBa_E0044
sequence annotation: BBa_B0010
sequence annotation: polya
sequence annotation: BBa_I732006
sequence annotation: STOP
sequence: https://synbiohub.org/public/igem/BBa_I732407_sequence/1

stop
type: SequenceAnnotation
persistent identity: annotation1944496
display id: annotation1944496
version: 1
top level: U097NUL+D0
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_I732407/annotation1944496/range1944496/1

https://synbiohub.org/public/igem/BBa_I732407/annotation1944496/range1944496/1
type: Range
persistent identity: range1944496
display id: range1944496
version: 1
direction: direction/forward
top level: U097NUL+D0
start: 1194
end: 1194
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation1944492
display id: annotation1944492
version: 1
top level: U097NUL+D0
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_I732407/annotation1944492/range1944492/1

https://synbiohub.org/public/igem/BBa_I732407/annotation1944492/range1944492/1
type: Range
persistent identity: range1944492
display id: range1944492
version: 1
direction: direction/forward
top level: U097NUL+D0
start: 1084
end: 1127
orientation: inline

conserved
type: SequenceAnnotation
persistent identity: annotation1944482
display id: annotation1944482
version: 1
top level: U097NUL+D0
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_I732407/annotation1944482/range1944482/1

https://synbiohub.org/public/igem/BBa_I732407/annotation1944482/range1944482/1
type: Range
persistent identity: range1944482
display id: range1944482
version: 1
direction: direction/forward
top level: U097NUL+D0
start: 32
end: 35
orientation: inline

START
type: SequenceAnnotation
persistent identity: annotation1944484
display id: annotation1944484
version: 1
top level: U097NUL+D0
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_I732407/annotation1944484/range1944484/1

https://synbiohub.org/public/igem/BBa_I732407/annotation1944484/range1944484/1
type: Range
persistent identity: range1944484
display id: range1944484
version: 1
direction: direction/forward
top level: U097NUL+D0
start: 46
end: 48
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1944493
display id: annotation1944493
version: 1
top level: U097NUL+D0
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_I732407/annotation1944493/range1944493/1

https://synbiohub.org/public/igem/BBa_I732407/annotation1944493/range1944493/1
type: Range
persistent identity: range1944493
display id: range1944493
version: 1
direction: direction/forward
top level: U097NUL+D0
start: 1161
end: 1201
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1944494
display id: annotation1944494
version: 1
top level: U097NUL+D0
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_I732407/annotation1944494/range1944494/1

https://synbiohub.org/public/igem/BBa_I732407/annotation1944494/range1944494/1
type: Range
persistent identity: range1944494
display id: range1944494
version: 1
direction: direction/forward
top level: U097NUL+D0
start: 1168
end: 1187
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1944489
display id: annotation1944489
version: 1
top level: U097NUL+D0
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_I732407/annotation1944489/range1944489/1

https://synbiohub.org/public/igem/BBa_I732407/annotation1944489/range1944489/1
type: Range
persistent identity: range1944489
display id: range1944489
version: 1
direction: direction/reverse
top level: U097NUL+D0
start: 288
end: 299
orientation: inline

BamHI
type: SequenceAnnotation
persistent identity: annotation1944480
display id: annotation1944480
version: 1
top level: U097NUL+D0
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I732407/annotation1944480/range1944480/1

https://synbiohub.org/public/igem/BBa_I732407/annotation1944480/range1944480/1
type: Range
persistent identity: range1944480
display id: range1944480
version: 1
direction: direction/forward
top level: U097NUL+D0
start: 12
end: 17
orientation: inline

lacZ
type: SequenceAnnotation
persistent identity: annotation1944485
display id: annotation1944485
version: 1
top level: U097NUL+D0
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_I732407/annotation1944485/range1944485/1

https://synbiohub.org/public/igem/BBa_I732407/annotation1944485/range1944485/1
type: Range
persistent identity: range1944485
display id: range1944485
version: 1
direction: direction/forward
top level: U097NUL+D0
start: 46
end: 279
orientation: inline

conserved
type: SequenceAnnotation
persistent identity: annotation1944488
display id: annotation1944488
version: 1
top level: U097NUL+D0
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_I732407/annotation1944488/range1944488/1

https://synbiohub.org/public/igem/BBa_I732407/annotation1944488/range1944488/1
type: Range
persistent identity: range1944488
display id: range1944488
version: 1
direction: direction/forward
top level: U097NUL+D0
start: 292
end: 295
orientation: inline

BBa_I732007
type: SequenceAnnotation
persistent identity: annotation1944481
display id: annotation1944481
version: 1
top level: U097NUL+D0
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_I732407/annotation1944481/range1944481/1

https://synbiohub.org/public/igem/BBa_I732407/annotation1944481/range1944481/1
type: Range
persistent identity: range1944481
display id: range1944481
version: 1
direction: direction/reverse
top level: U097NUL+D0
start: 1
end: 19
orientation: inline

BglII
type: SequenceAnnotation
persistent identity: annotation1944479
display id: annotation1944479
version: 1
top level: U097NUL+D0
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I732407/annotation1944479/range1944479/1

https://synbiohub.org/public/igem/BBa_I732407/annotation1944479/range1944479/1
type: Range
persistent identity: range1944479
display id: range1944479
version: 1
direction: direction/forward
top level: U097NUL+D0
start: 3
end: 8
orientation: inline

BBa_E0044
type: SequenceAnnotation
persistent identity: annotation1944490
display id: annotation1944490
version: 1
top level: U097NUL+D0
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_I732407/annotation1944490/range1944490/1

https://synbiohub.org/public/igem/BBa_I732407/annotation1944490/range1944490/1
type: Range
persistent identity: range1944490
display id: range1944490
version: 1
direction: direction/reverse
top level: U097NUL+D0
start: 306
end: 1064
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1944491
display id: annotation1944491
version: 1
top level: U097NUL+D0
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_I732407/annotation1944491/range1944491/1

https://synbiohub.org/public/igem/BBa_I732407/annotation1944491/range1944491/1
type: Range
persistent identity: range1944491
display id: range1944491
version: 1
direction: direction/forward
top level: U097NUL+D0
start: 1073
end: 1152
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1944495
display id: annotation1944495
version: 1
top level: U097NUL+D0
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_I732407/annotation1944495/range1944495/1

https://synbiohub.org/public/igem/BBa_I732407/annotation1944495/range1944495/1
type: Range
persistent identity: range1944495
display id: range1944495
version: 1
direction: direction/forward
top level: U097NUL+D0
start: 1188
end: 1201
orientation: inline

BBa_I732006
type: SequenceAnnotation
persistent identity: annotation1944487
display id: annotation1944487
version: 1
top level: U097NUL+D0
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_I732407/annotation1944487/range1944487/1

https://synbiohub.org/public/igem/BBa_I732407/annotation1944487/range1944487/1
type: Range
persistent identity: range1944487
display id: range1944487
version: 1
direction: direction/reverse
top level: U097NUL+D0
start: 46
end: 279
orientation: inline

STOP
type: SequenceAnnotation
persistent identity: annotation1944486
display id: annotation1944486
version: 1
top level: U097NUL+D0
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_I732407/annotation1944486/range1944486/1

https://synbiohub.org/public/igem/BBa_I732407/annotation1944486/range1944486/1
type: Range
persistent identity: range1944486
display id: range1944486
version: 1
direction: direction/forward
top level: U097NUL+D0
start: 274
end: 279
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1944483
display id: annotation1944483
version: 1
top level: U097NUL+D0
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_I732407/annotation1944483/range1944483/1

https://synbiohub.org/public/igem/BBa_I732407/annotation1944483/range1944483/1
type: Range
persistent identity: range1944483
display id: range1944483
version: 1
direction: direction/reverse
top level: U097NUL+D0
start: 28
end: 39
orientation: inline

BBa_K137029
type: ComponentDefinition
persistent identity: BBa K137029
display id: BBa_K137029
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: constitutive promoter with (TA)10 between -10 and -35 elements
creator: Allen Lin
created: 2008-07-20T11:00:00Z
modified: 2015-05-08T01:10:09Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _187_
m user id: 0
owner id: 3112
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: constitutive promoter with (TA)10 between -10 and -35 elements
mutable notes: With 10 TA repeats between the -10 and -35 elements, the distance between the two elements is optimal for the sigma factor to bind to the promoter. Thus, the promoter should be in the 'on' state.
mutable provenance: This part was PCR amplified from two synthesized primers that bound to each other.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: TA repeat
sequence annotation: -10 element
sequence: https://synbiohub.org/public/igem/BBa_K137029_sequence/1

TA repeat
type: SequenceAnnotation
persistent identity: annotation1967765
display id: annotation1967765
version: 1
top level: BBa_K137029
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K137029/annotation1967765/range1967765/1

https://synbiohub.org/public/igem/BBa_K137029/annotation1967765/range1967765/1
type: Range
persistent identity: range1967765
display id: range1967765
version: 1
direction: direction/forward
top level: BBa_K137029
start: 7
end: 22
orientation: inline

-10 element
type: SequenceAnnotation
persistent identity: annotation1967764
display id: annotation1967764
version: 1
top level: BBa_K137029
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K137029/annotation1967764/range1967764/1

https://synbiohub.org/public/igem/BBa_K137029/annotation1967764/range1967764/1
type: Range
persistent identity: range1967764
display id: range1967764
version: 1
direction: direction/forward
top level: BBa_K137029
start: 23
end: 28
orientation: inline

BBa_K180005
type: ComponentDefinition
persistent identity: BBa K180005
display id: BBa_K180005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: GoL - Primary plasmid (part 1)/RPS - Paper primary plasmid (part 1) [LuxR generator]
creator: Richard Wilson, Stephanie Chambers
created: 2009-10-15T11:00:00Z
modified: 2015-05-08T01:11:06Z
discontinued: false
dominant: false
experience: experience/None
group u list: _278_
m user id: 0
owner id: 4082
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Part of the iGEM2009 Team:Southampton Game of Life primary plasmid.
mutable notes: Constitutive promoter selected to allow for continuous expression of C0062.
mutable provenance: See relevant basic biobrick parts.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0034
component: BBa_J23119
component: BBa_B0015
component: BBa_C0062
sequence annotation: BBa_B0015
sequence annotation: BBa_B0034
sequence annotation: BBa_J23119
sequence annotation: BBa_C0062
sequence: https://synbiohub.org/public/igem/BBa_K180005_sequence/1

BBa_B0034
type: Component
persistent identity: component2255826
display id: component2255826
version: 1
top level: BBa_K180005
access: public
definition: BBa_B0034

BBa_J23119
type: Component
persistent identity: component2255824
display id: component2255824
version: 1
top level: BBa_K180005
access: public
definition: BBa_J23119

BBa_B0015
type: Component
persistent identity: component2255839
display id: component2255839
version: 1
top level: BBa_K180005
access: public
definition: BBa_B0015

BBa_C0062
type: Component
persistent identity: component2255829
display id: component2255829
version: 1
top level: BBa_K180005
access: public
definition: https://synbiohub.org/public/igem/BBa_C0062/1

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2255839
display id: annotation2255839
version: 1
top level: BBa_K180005
location: https://synbiohub.org/public/igem/BBa_K180005/annotation2255839/range2255839/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K180005/annotation2255839/range2255839/1
type: Range
persistent identity: range2255839
display id: range2255839
version: 1
direction: direction/reverse
top level: BBa_K180005
start: 851
end: 979
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2255826
display id: annotation2255826
version: 1
top level: BBa_K180005
location: https://synbiohub.org/public/igem/BBa_K180005/annotation2255826/range2255826/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K180005/annotation2255826/range2255826/1
type: Range
persistent identity: range2255826
display id: range2255826
version: 1
direction: direction/reverse
top level: BBa_K180005
start: 44
end: 55
orientation: inline

BBa_J23119
type: SequenceAnnotation
persistent identity: annotation2255824
display id: annotation2255824
version: 1
top level: BBa_K180005
location: https://synbiohub.org/public/igem/BBa_K180005/annotation2255824/range2255824/1
component: BBa_J23119

https://synbiohub.org/public/igem/BBa_K180005/annotation2255824/range2255824/1
type: Range
persistent identity: range2255824
display id: range2255824
version: 1
direction: direction/reverse
top level: BBa_K180005
start: 1
end: 35
orientation: inline

BBa_C0062
type: SequenceAnnotation
persistent identity: annotation2255829
display id: annotation2255829
version: 1
top level: BBa_K180005
location: https://synbiohub.org/public/igem/BBa_K180005/annotation2255829/range2255829/1
component: BBa_C0062

https://synbiohub.org/public/igem/BBa_K180005/annotation2255829/range2255829/1
type: Range
persistent identity: range2255829
display id: range2255829
version: 1
direction: direction/forward
top level: BBa_K180005
start: 62
end: 817
orientation: inline

BBa_K863200
type: ComponentDefinition
persistent identity: BBa K863200
display id: BBa_K863200
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: 5' UTR site of alcohol oxidase 1 gene (aox1)
creator: Julia Schirmacher
created: 2012-09-19T11:00:00Z
modified: 2015-05-08T01:13:36Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1123_
m user id: 0
owner id: 13076
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: 5' UTR site of alcohol oxidase 1 gene (AOX1) from K. pastoris used as a recombination site.
mutable notes: coming soon
mutable provenance: K. pastoris
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Other
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_K863200_sequence/1

BBa_K658008
type: ComponentDefinition
persistent identity: BBa K658008
display id: BBa_K658008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: position 3&5 mutated promoter lux pR-3/5 (luxR & HSL regulated)
creator: Yidan Hu
created: 2011-09-30T11:00:00Z
modified: 2015-05-08T01:13:02Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _835_
m user id: 0
owner id: 10207
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: The position 3&5 mutated promoter lux pR-3/5 is a product of site-directed mutagenesis of promoter lux pR (BBa_R0062). The promoter lux pR is based on the Vibrio fischeri quorum sensing gene promoters. The position 5 mutated promoter lux pR-3/5 gives a lower expression of downstream genes in an autoinduction system. In another word, the lux pR-3/5 has a lower efficiency than the lux pR(BBa_R0062). Similar to promoter lux pR, the expression is up-regulated by the action of LuxR protein combined with the signal molecule HSL.
mutable notes: We have generated two point mutants by site-directed mutagenesis from the sequence of lux pR(BBa_R0062). In these mutants, the G residue of position 5 was changed to a C and the C residue of position 3 was changed to a T respectively.
mutable provenance: The promoter lux pR (BBa_R0062).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: LuxR/HSL
sequence annotation: K658008
sequence annotation: -35
sequence annotation: start
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_K658008_sequence/1

LuxR/HSL
type: SequenceAnnotation
persistent identity: annotation2148176
display id: annotation2148176
version: 1
top level: BBa_K658008
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K658008/annotation2148176/range2148176/1

https://synbiohub.org/public/igem/BBa_K658008/annotation2148176/range2148176/1
type: Range
persistent identity: range2148176
display id: range2148176
version: 1
direction: direction/forward
top level: BBa_K658008
start: 1
end: 20
orientation: inline

K658008
type: SequenceAnnotation
persistent identity: annotation2148175
display id: annotation2148175
version: 1
top level: BBa_K658008
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K658008/annotation2148175/range2148175/1

https://synbiohub.org/public/igem/BBa_K658008/annotation2148175/range2148175/1
type: Range
persistent identity: range2148175
display id: range2148175
version: 1
direction: direction/forward
top level: BBa_K658008
start: 1
end: 55
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2148173
display id: annotation2148173
version: 1
top level: BBa_K658008
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K658008/annotation2148173/range2148173/1

https://synbiohub.org/public/igem/BBa_K658008/annotation2148173/range2148173/1
type: Range
persistent identity: range2148173
display id: range2148173
version: 1
direction: direction/forward
top level: BBa_K658008
start: 20
end: 25
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2148172
display id: annotation2148172
version: 1
top level: BBa_K658008
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K658008/annotation2148172/range2148172/1

https://synbiohub.org/public/igem/BBa_K658008/annotation2148172/range2148172/1
type: Range
persistent identity: range2148172
display id: range2148172
version: 1
direction: direction/forward
top level: BBa_K658008
start: 53
end: 53
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2148174
display id: annotation2148174
version: 1
top level: BBa_K658008
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K658008/annotation2148174/range2148174/1

https://synbiohub.org/public/igem/BBa_K658008/annotation2148174/range2148174/1
type: Range
persistent identity: range2148174
display id: range2148174
version: 1
direction: direction/forward
top level: BBa_K658008
start: 42
end: 47
orientation: inline

creB RBS
type: ComponentDefinition
persistent identity: BBa J64968
display id: BBa_J64968
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS on creB
creator: Kristopher Kuchenbecker
created: 2007-03-19T12:00:00Z
modified: 2015-05-08T01:08:17Z
discontinued: false
dominant: false
experience: experience/None
group u list: _98_
m user id: 0
owner id: 1429
owning group id: 98
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Ribosome Binding Site
mutable notes: RBS
mutable provenance: ''E. coli'' K-12 genome
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J64968_sequence/1

BBa_J61132
type: ComponentDefinition
persistent identity: BBa J61132
display id: BBa_J61132
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Ribosome Binding Site Family Member
creator: John Anderson
created: 2007-04-24T11:00:00Z
modified: 2015-08-31T01:59:49Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: false
mutable description: {{JCA_Arkin_RBSFamily}}
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J61132_sequence/1

BBa_I742153
type: ComponentDefinition
persistent identity: BBa I742153
display id: BBa_I742153
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: crtY native rbs
creator: Chris French
created: 2007-10-23T11:00:00Z
modified: 2015-08-31T04:08:03Z
discontinued: false
dominant: false
experience: experience/None
group u list: _123_
m user id: 0
owner id: 837
owning group id: 163
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Native ribosome binding site of crtY (lycopene cyclase) from Pantoea ananatis (formerly Erwinia uredovora) DSMZ 30080 (ATCC 19321). Accession: D90087.
mutable notes: This is a virtual part to be added, blunt-ended, to the coding sequence.
mutable provenance: Pantoea ananatis (formerly Erwinia uredovora) DSMZ 30080 (ATCC 19321). Accession: D90087.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_I742153_sequence/1

BBa_K110005
type: ComponentDefinition
persistent identity: BBa K110005
display id: BBa_K110005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Alpha-Cell Promoter MF(ALPHA)2
creator: James DiCarlo
created: 2008-05-29T11:00:00Z
modified: 2015-05-08T01:09:08Z
discontinued: false
dominant: false
experience: experience/None
group u list: _201_
m user id: 0
owner id: 2669
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This promoter was isolated by taking 500bp upstream of the MF(ALPHA)2 gene of S. cerevisiae. This promoter will be activated when alpha factor is expressed in an alpha haploid yeast cell.
mutable notes: This gene is on the crick strand of the entered sequence.
mutable provenance: SGD
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: MCM1
sequence annotation: MF(alpha)2
sequence: https://synbiohub.org/public/igem/BBa_K110005_sequence/1

MCM1
type: SequenceAnnotation
persistent identity: annotation1997684
display id: annotation1997684
version: 1
top level: BBa_K110005
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K110005/annotation1997684/range1997684/1

https://synbiohub.org/public/igem/BBa_K110005/annotation1997684/range1997684/1
type: Range
persistent identity: range1997684
display id: range1997684
version: 1
direction: direction/forward
top level: BBa_K110005
start: 184
end: 209
orientation: inline

MF(alpha)2
type: SequenceAnnotation
persistent identity: annotation1997686
display id: annotation1997686
version: 1
top level: BBa_K110005
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K110005/annotation1997686/range1997686/1

https://synbiohub.org/public/igem/BBa_K110005/annotation1997686/range1997686/1
type: Range
persistent identity: range1997686
display id: range1997686
version: 1
direction: direction/forward
top level: BBa_K110005
start: 1
end: 500
orientation: inline

BBa_K1480005
type: ComponentDefinition
persistent identity: BBa K1480005
display id: BBa_K1480005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TetR generator (Promoter J23111 )
creator: Jesus Gilberto Rodriguez Ceja
created: 2014-10-13T11:00:00Z
modified: 2015-05-08T01:10:40Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1860_
m user id: 0
owner id: 12878
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is a TetR protein generator regulated by the constitutive promoter J23111. The protein has a LVA tag for degradation.
mutable notes: It was assembled using assembly 10.
mutable provenance: Based on parts of the registry.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0034
component: BBa_C0040
component: BBa_J23111
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_B0010
sequence annotation: BBa_B0034
sequence annotation: BBa_C0040
sequence annotation: BBa_J23111
sequence: https://synbiohub.org/public/igem/BBa_K1480005_sequence/1

BBa_B0034
type: Component
persistent identity: component2419556
display id: component2419556
version: 1
top level: BBa_K1480005
access: public
definition: BBa_B0034

BBa_C0040
type: Component
persistent identity: component2419560
display id: component2419560
version: 1
top level: BBa_K1480005
access: public
definition: https://synbiohub.org/public/igem/BBa_C0040/1

BBa_J23111
type: Component
persistent identity: component2419554
display id: component2419554
version: 1
top level: BBa_K1480005
access: public
definition: BBa_J23111

BBa_B0012
type: Component
persistent identity: component2419563
display id: component2419563
version: 1
top level: BBa_K1480005
access: public
definition: BBa_B0012

BBa_B0010
type: Component
persistent identity: component2419561
display id: component2419561
version: 1
top level: BBa_K1480005
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2419563
display id: annotation2419563
version: 1
top level: BBa_K1480005
location: https://synbiohub.org/public/igem/BBa_K1480005/annotation2419563/range2419563/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K1480005/annotation2419563/range2419563/1
type: Range
persistent identity: range2419563
display id: range2419563
version: 1
direction: direction/forward
top level: BBa_K1480005
start: 843
end: 883
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2419561
display id: annotation2419561
version: 1
top level: BBa_K1480005
location: https://synbiohub.org/public/igem/BBa_K1480005/annotation2419561/range2419561/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K1480005/annotation2419561/range2419561/1
type: Range
persistent identity: range2419561
display id: range2419561
version: 1
direction: direction/forward
top level: BBa_K1480005
start: 755
end: 834
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2419556
display id: annotation2419556
version: 1
top level: BBa_K1480005
location: https://synbiohub.org/public/igem/BBa_K1480005/annotation2419556/range2419556/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1480005/annotation2419556/range2419556/1
type: Range
persistent identity: range2419556
display id: range2419556
version: 1
direction: direction/reverse
top level: BBa_K1480005
start: 44
end: 55
orientation: inline

BBa_C0040
type: SequenceAnnotation
persistent identity: annotation2419560
display id: annotation2419560
version: 1
top level: BBa_K1480005
location: https://synbiohub.org/public/igem/BBa_K1480005/annotation2419560/range2419560/1
component: BBa_C0040

https://synbiohub.org/public/igem/BBa_K1480005/annotation2419560/range2419560/1
type: Range
persistent identity: range2419560
display id: range2419560
version: 1
direction: direction/reverse
top level: BBa_K1480005
start: 62
end: 746
orientation: inline

BBa_J23111
type: SequenceAnnotation
persistent identity: annotation2419554
display id: annotation2419554
version: 1
top level: BBa_K1480005
location: https://synbiohub.org/public/igem/BBa_K1480005/annotation2419554/range2419554/1
component: BBa_J23111

https://synbiohub.org/public/igem/BBa_K1480005/annotation2419554/range2419554/1
type: Range
persistent identity: range2419554
display id: range2419554
version: 1
direction: direction/reverse
top level: BBa_K1480005
start: 1
end: 35
orientation: inline

BBa_J23118
type: ComponentDefinition
persistent identity: BBa J23118
display id: BBa_J23118
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: constitutive promoter family member
creator: John Anderson
created: 2006-08-16T11:00:00Z
modified: 2015-08-31T04:08:40Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: Later
mutable notes: N/A
mutable provenance: Later
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J23118_sequence/1

fhuA
type: ComponentDefinition
persistent identity: BBa K259000
display id: BBa_K259000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: fhuA - Outer membrane transporter for ferrichrome-iron
creator: Petros Mina
created: 2009-07-13T11:00:00Z
modified: 2015-05-08T01:11:42Z
discontinued: false
dominant: true
experience: experience/None
group u list: _357_
m user id: 0
owner id: 5318
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: fhuA is an outer membrane (OM) protein that is found in the gram negative bacterium, E.coli. It is involved in the active-transport (energy-dependent uptake) of ferrichrome-iron from the extracellular environment. However fhuA is only able to bind ferrichrome-iron (i.e. ferrichrome iron is a ligand of fhuA protein). For the uptake to be completed fhuA needs to form a complex with another protein, tonB that carries out the energy-dependent uptake by using ATP as substrate to undergo the relevant conformational changes. This biobrick ONLY encodes for fhuA. fhuA is a beta-barell protein (22 anti-parallel beta-strands) and is composed of 747 aminoacids. This receptor protein may be used as an attachment point by some colicins. References: Locher K.P., Rees B., Koebnik R., Mitschler A., Moulinier L., Rosenusch J.P. and Moras D. (1998),Transmembrane signalling across the Ligand-Gated FhuAReceptor: Crystal structures of Free and Ferrichrome-Bound States reveal allosteric changes. Cell 95,771-778. Uniprot access number: P06971 Ecogene: http://ecogene.org/geneInfo.php?eg_id=EG10302
mutable notes: The coding sequence (CDS) was already in compliance with assembly standard 10 (RFC10).
mutable provenance: fhuA is part of the chromosomal genome of E.coli. In particular it can be found at 3.61min and follows the clockwise direction.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: stop
sequence annotation: Multipass (OM-Perpiplasm) protein
sequence annotation: Signal Sequence
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_K259000_sequence/1

stop
type: SequenceAnnotation
persistent identity: annotation2011291
display id: annotation2011291
version: 1
top level: fhuA
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K259000/annotation2011291/range2011291/1

https://synbiohub.org/public/igem/BBa_K259000/annotation2011291/range2011291/1
type: Range
persistent identity: range2011291
display id: range2011291
version: 1
direction: direction/forward
top level: fhuA
start: 2242
end: 2247
orientation: inline

Multipass (OM-Perpiplasm) protein
type: SequenceAnnotation
persistent identity: annotation2011311
display id: annotation2011311
version: 1
top level: fhuA
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K259000/annotation2011311/range2011311/1

https://synbiohub.org/public/igem/BBa_K259000/annotation2011311/range2011311/1
type: Range
persistent identity: range2011311
display id: range2011311
version: 1
direction: direction/forward
top level: fhuA
start: 100
end: 2241
orientation: inline

Signal Sequence
type: SequenceAnnotation
persistent identity: annotation2011310
display id: annotation2011310
version: 1
top level: fhuA
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K259000/annotation2011310/range2011310/1

https://synbiohub.org/public/igem/BBa_K259000/annotation2011310/range2011310/1
type: Range
persistent identity: range2011310
display id: range2011310
version: 1
direction: direction/forward
top level: fhuA
start: 1
end: 99
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2011290
display id: annotation2011290
version: 1
top level: fhuA
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K259000/annotation2011290/range2011290/1

https://synbiohub.org/public/igem/BBa_K259000/annotation2011290/range2011290/1
type: Range
persistent identity: range2011290
display id: range2011290
version: 1
direction: direction/forward
top level: fhuA
start: 1
end: 3
orientation: inline

BBa_J45504
type: ComponentDefinition
persistent identity: BBa J45504
display id: BBa_J45504
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: htpG Heat Shock Promoter
creator: Stephen Payne
created: 2006-06-20T11:00:00Z
modified: 2015-08-31T04:08:49Z
discontinued: false
dominant: false
group u list: _84_
m user id: 0
owner id: 642
owning group id: 84
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This promoter is active at higher temperatures.
mutable notes: None.
mutable provenance: Chris Voigt Lab at UCSF
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J45504_sequence/1

BBa_J64022
type: ComponentDefinition
persistent identity: BBa J64022
display id: BBa_J64022
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: sicA RBS
creator: Dan Widmaier
created: 2008-01-16T12:00:00Z
modified: 2015-08-31T01:56:26Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _98_
m user id: 0
owner id: 2407
owning group id: 98
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is the natural RBS from the sicA chaperone (BBa_J6021) in the Salmonella Type III Secretion System.
mutable notes: This part was not designed with standard biobrick assembly in mind.
mutable provenance: Salmonella LT2 Genomic DNA
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: misc
sequence: https://synbiohub.org/public/igem/BBa_J64022_sequence/1

misc
type: SequenceAnnotation
persistent identity: annotation1959621
display id: annotation1959621
version: 1
top level: BBa_J64022
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J64022/annotation1959621/range1959621/1

https://synbiohub.org/public/igem/BBa_J64022/annotation1959621/range1959621/1
type: Range
persistent identity: range1959621
display id: range1959621
version: 1
direction: direction/forward
top level: BBa_J64022
start: 1
end: 20
orientation: inline

U085O11+D0
type: ComponentDefinition
persistent identity: BBa I732402
display id: BBa_I732402
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Family Member (U085O11+D062NUL)
creator: Zhan Jian
created: 2007-09-20T11:00:00Z
modified: 2015-08-31T04:07:58Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1572
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Might be later edited.
mutable notes: Might be later edited.
mutable provenance: Might be later edited.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: consensus elements
sequence annotation: +1
sequence annotation: O1
sequence: https://synbiohub.org/public/igem/BBa_I732402_sequence/1

consensus elements
type: SequenceAnnotation
persistent identity: annotation1944539
display id: annotation1944539
version: 1
top level: U085O11+D0
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I732402/annotation1944539/range1944539/1

https://synbiohub.org/public/igem/BBa_I732402/annotation1944539/range1944539/1
type: Range
persistent identity: range1944539
display id: range1944539
version: 1
direction: direction/forward
top level: U085O11+D0
start: 69
end: 105
orientation: inline

+1
type: SequenceAnnotation
persistent identity: annotation1944541
display id: annotation1944541
version: 1
top level: U085O11+D0
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_I732402/annotation1944541/range1944541/1

https://synbiohub.org/public/igem/BBa_I732402/annotation1944541/range1944541/1
type: Range
persistent identity: range1944541
display id: range1944541
version: 1
direction: direction/forward
top level: U085O11+D0
start: 105
end: 105
orientation: inline

O1
type: SequenceAnnotation
persistent identity: annotation1944540
display id: annotation1944540
version: 1
top level: U085O11+D0
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_I732402/annotation1944540/range1944540/1

https://synbiohub.org/public/igem/BBa_I732402/annotation1944540/range1944540/1
type: Range
persistent identity: range1944540
display id: range1944540
version: 1
direction: direction/forward
top level: U085O11+D0
start: 1
end: 20
orientation: inline

pTEF
type: ComponentDefinition
persistent identity: BBa K165037
display id: BBa_K165037
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TEF2 yeast constitutive promoter
creator: John Szymanski
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:10:56Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _267_
m user id: 0
owner id: 2512
owning group id: 58
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: This is a high level constitutive promoter from the yeast gene TEF2.
mutable notes: -
mutable provenance: From the yeast genome using promoter definition from: Mumberg, Muller, and Funk: http://dx.doi.org/10.1016/0378-1119(95)00037-7
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K165037_sequence/1

BBa_J34814
type: ComponentDefinition
persistent identity: BBa J34814
display id: BBa_J34814
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: T7 Promoter
creator: Alexandra Choutko
created: 2006-10-25T11:00:00Z
modified: 2015-08-31T04:08:47Z
discontinued: false
dominant: false
experience: experience/None
group u list: _62_
m user id: 0
owner id: 1085
owning group id: 62
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: to be used qith t7 polymerase BBa_J34811
mutable notes: a
mutable provenance: a
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J34814_sequence/1

BBa_B0015
type: ComponentDefinition
persistent identity: BBa B0015
display id: BBa_B0015
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: double terminator (B0010-B0012)
creator: Reshma Shetty
created: 2003-07-16T11:00:00Z
modified: 2015-08-31T04:07:20Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Double terminator consisting of BBa_B0010 and BBa_B0012
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000141
role: partType/Terminator
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_B0010
sequence: https://synbiohub.org/public/igem/BBa_B0015_sequence/1

BBa_B0012
type: Component
persistent identity: component1916612
display id: component1916612
version: 1
top level: BBa_B0015
access: public
definition: BBa_B0012

BBa_B0010
type: Component
persistent identity: component1916610
display id: component1916610
version: 1
top level: BBa_B0015
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1916612
display id: annotation1916612
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
type: Range
persistent identity: range1916612
display id: range1916612
version: 1
direction: direction/forward
top level: BBa_B0015
start: 89
end: 129
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1916610
display id: annotation1916610
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
type: Range
persistent identity: range1916610
display id: range1916610
version: 1
direction: direction/forward
top level: BBa_B0015
start: 1
end: 80
orientation: inline

BBa_K266009
type: ComponentDefinition
persistent identity: BBa K266009
display id: BBa_K266009
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: J23100 + Lac inverter + RBS
creator: UNAM FCIENCIAS-CINVESTAV IRA
created: 2009-10-12T11:00:00Z
modified: 2015-05-08T01:11:43Z
discontinued: false
dominant: false
experience: experience/None
group u list: _363_
m user id: 0
owner id: 1955
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: The BBa_J23100 constitutive promoter with Lac systems inverter and strong RBS
mutable notes: None
mutable provenance: The biobricks BBa_K266008 and BBa_B0034 where used to build this part
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Inverter
component: BBa_K266008
component: BBa_B0034
sequence annotation: BBa_B0034
sequence annotation: BBa_K266008
sequence: https://synbiohub.org/public/igem/BBa_K266009_sequence/1

BBa_K266008
type: Component
persistent identity: component2267639
display id: component2267639
version: 1
top level: BBa_K266009
access: public
definition: BBa_K266008

BBa_B0034
type: Component
persistent identity: component2267641
display id: component2267641
version: 1
top level: BBa_K266009
access: public
definition: BBa_B0034

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2267641
display id: annotation2267641
version: 1
top level: BBa_K266009
location: https://synbiohub.org/public/igem/BBa_K266009/annotation2267641/range2267641/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K266009/annotation2267641/range2267641/1
type: Range
persistent identity: range2267641
display id: range2267641
version: 1
direction: direction/reverse
top level: BBa_K266009
start: 1423
end: 1434
orientation: inline

BBa_K266008
type: SequenceAnnotation
persistent identity: annotation2267639
display id: annotation2267639
version: 1
top level: BBa_K266009
location: https://synbiohub.org/public/igem/BBa_K266009/annotation2267639/range2267639/1
component: BBa_K266008

https://synbiohub.org/public/igem/BBa_K266009/annotation2267639/range2267639/1
type: Range
persistent identity: range2267639
display id: range2267639
version: 1
direction: direction/reverse
top level: BBa_K266009
start: 1
end: 1414
orientation: inline

BBa_K165030
type: ComponentDefinition
persistent identity: BBa K165030
display id: BBa_K165030
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: mCYC promoter plus Zif268-HIV binding sites
creator: Aaron Glieberman
created: 2008-10-28T12:00:00Z
modified: 2015-05-08T01:10:56Z
discontinued: false
dominant: false
group u list: _267_
m user id: 0
owner id: 2510
owning group id: 58
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This is a composite part containing the minimal promoter mCYC preceded by multiple consecutive binding sites for the Zif268-HIV binding domain. It contains the sequences of parts BBa_K165011 and BBa_K165016 but also contains additional DNA.
mutable notes: It was necessary to design primers for use in PCR to remove this part from its host plasmid and transfer it to a standard Biobrick vector.
mutable provenance: Composite part that was taken via PCR from a synthetic plasmid containing the sequence. The plasmid came from Lawrence Berkeley National Laboratory.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: Zif268-HIV binding sites (3)
sequence: https://synbiohub.org/public/igem/BBa_K165030_sequence/1

Zif268-HIV binding sites (3)
type: SequenceAnnotation
persistent identity: annotation1994037
display id: annotation1994037
version: 1
top level: BBa_K165030
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K165030/annotation1994037/range1994037/1

https://synbiohub.org/public/igem/BBa_K165030/annotation1994037/range1994037/1
type: Range
persistent identity: range1994037
display id: range1994037
version: 1
direction: direction/forward
top level: BBa_K165030
start: 1
end: 46
orientation: inline

BBa_S04209
type: ComponentDefinition
persistent identity: BBa S04209
display id: BBa_S04209
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: R0051:Q04121:B0034:C0079:B0015
creator: UNAM FCiencias-CINVESTAV IRA
created: 2009-06-02T11:00:00Z
modified: 2015-05-08T01:14:36Z
discontinued: false
dominant: true
experience: experience/None
group u list: _9_
m user id: 0
owner id: 1955
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_S04208
component: BBa_B0034
sequence annotation: BBa_S04208
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_S04209_sequence/1

BBa_S04208
type: Component
persistent identity: component2267293
display id: component2267293
version: 1
top level: BBa_S04209
access: public
definition: https://synbiohub.org/public/igem/BBa_S04208/1

BBa_B0034
type: Component
persistent identity: component2267295
display id: component2267295
version: 1
top level: BBa_S04209
access: public
definition: BBa_B0034

BBa_S04208
type: SequenceAnnotation
persistent identity: annotation2267293
display id: annotation2267293
version: 1
top level: BBa_S04209
location: https://synbiohub.org/public/igem/BBa_S04209/annotation2267293/range2267293/1
component: BBa_S04208

https://synbiohub.org/public/igem/BBa_S04209/annotation2267293/range2267293/1
type: Range
persistent identity: range2267293
display id: range2267293
version: 1
direction: direction/reverse
top level: BBa_S04209
start: 1
end: 1414
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2267295
display id: annotation2267295
version: 1
top level: BBa_S04209
location: https://synbiohub.org/public/igem/BBa_S04209/annotation2267295/range2267295/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_S04209/annotation2267295/range2267295/1
type: Range
persistent identity: range2267295
display id: range2267295
version: 1
direction: direction/reverse
top level: BBa_S04209
start: 1423
end: 1434
orientation: inline

BBa_K105028
type: ComponentDefinition
persistent identity: BBa K105028
display id: BBa_K105028
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: cyc70 minimal promoter
creator: Michael Wild
created: 2008-10-25T11:00:00Z
modified: 2015-05-08T01:08:52Z
discontinued: false
dominant: false
experience: experience/None
group u list: _253_
m user id: 0
owner id: 3313
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This is a derivate of BBa_K105027. Due to a point mutation in the TATA box region the efficiency of this minimal promoter is only 70 % of the natural yeast CYC1 promoter.
mutable notes: This BioBrick contains the TATA-box with one point mutation and most of the 5'UTR (-139 to -36, relative to the translational start) of the natural CYC1 promoter. A Kozak sequence is not included and has thus to be added! Even if there isn't any need for this BioBrick to be fusible to other protein domains, we stayed with our design.
mutable provenance: Amplification and Mutation from the plasmid pAH3
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: A->C
sequence annotation: TATA-box
sequence: https://synbiohub.org/public/igem/BBa_K105028_sequence/1

A->C
type: SequenceAnnotation
persistent identity: annotation1989836
display id: annotation1989836
version: 1
top level: BBa_K105028
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_K105028/annotation1989836/range1989836/1

https://synbiohub.org/public/igem/BBa_K105028/annotation1989836/range1989836/1
type: Range
persistent identity: range1989836
display id: range1989836
version: 1
direction: direction/forward
top level: BBa_K105028
start: 24
end: 24
orientation: inline

TATA-box
type: SequenceAnnotation
persistent identity: annotation1989835
display id: annotation1989835
version: 1
top level: BBa_K105028
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K105028/annotation1989835/range1989835/1

https://synbiohub.org/public/igem/BBa_K105028/annotation1989835/range1989835/1
type: Range
persistent identity: range1989835
display id: range1989835
version: 1
direction: direction/forward
top level: BBa_K105028
start: 17
end: 23
orientation: inline

BBa_K089006
type: ComponentDefinition
persistent identity: BBa K089006
display id: BBa_K089006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: -663 to Tc start site of phaC
creator: Trent Mortensen
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:08:37Z
discontinued: false
dominant: false
group u list: _245_
m user id: 0
owner id: 3156
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: -663 to Tc start site of phaC
mutable notes: -663 to Tc start site of phaC
mutable provenance: Ralsotnia eutropha genomic DNA
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -35 site
sequence annotation: +1 site
sequence annotation: -10 site
sequence: https://synbiohub.org/public/igem/BBa_K089006_sequence/1

-35 site
type: SequenceAnnotation
persistent identity: annotation1996729
display id: annotation1996729
version: 1
top level: BBa_K089006
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K089006/annotation1996729/range1996729/1

https://synbiohub.org/public/igem/BBa_K089006/annotation1996729/range1996729/1
type: Range
persistent identity: range1996729
display id: range1996729
version: 1
direction: direction/forward
top level: BBa_K089006
start: 322
end: 327
orientation: inline

+1 site
type: SequenceAnnotation
persistent identity: annotation1996727
display id: annotation1996727
version: 1
top level: BBa_K089006
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K089006/annotation1996727/range1996727/1

https://synbiohub.org/public/igem/BBa_K089006/annotation1996727/range1996727/1
type: Range
persistent identity: range1996727
display id: range1996727
version: 1
direction: direction/forward
top level: BBa_K089006
start: 357
end: 357
orientation: inline

-10 site
type: SequenceAnnotation
persistent identity: annotation1996728
display id: annotation1996728
version: 1
top level: BBa_K089006
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K089006/annotation1996728/range1996728/1

https://synbiohub.org/public/igem/BBa_K089006/annotation1996728/range1996728/1
type: Range
persistent identity: range1996728
display id: range1996728
version: 1
direction: direction/forward
top level: BBa_K089006
start: 346
end: 351
orientation: inline

BBa_J64068
type: ComponentDefinition
persistent identity: BBa J64068
display id: BBa_J64068
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: increased strength R0051
creator: Jeffrey J Tabor
created: 2008-01-11T12:00:00Z
modified: 2015-05-08T01:08:17Z
discontinued: false
dominant: false
experience: experience/None
group u list: _98_
m user id: 0
owner id: 88
owning group id: 98
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: R0051 was modified to contain consensus -35 and -10 sequences. The CI operator OR1 was moved from it's original position (overlapping the -10) to between the -35 and -10. Fortuitously this results in the optimal (for high expression) 17bp spacing between the -35 and -10 sites.
mutable notes: none
mutable provenance: designed
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -35
sequence annotation: -10
sequence annotation: OR1
sequence: https://synbiohub.org/public/igem/BBa_J64068_sequence/1

-35
type: SequenceAnnotation
persistent identity: annotation1959539
display id: annotation1959539
version: 1
top level: BBa_J64068
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_J64068/annotation1959539/range1959539/1

https://synbiohub.org/public/igem/BBa_J64068/annotation1959539/range1959539/1
type: Range
persistent identity: range1959539
display id: range1959539
version: 1
direction: direction/forward
top level: BBa_J64068
start: 15
end: 20
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1959541
display id: annotation1959541
version: 1
top level: BBa_J64068
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_J64068/annotation1959541/range1959541/1

https://synbiohub.org/public/igem/BBa_J64068/annotation1959541/range1959541/1
type: Range
persistent identity: range1959541
display id: range1959541
version: 1
direction: direction/forward
top level: BBa_J64068
start: 38
end: 43
orientation: inline

OR1
type: SequenceAnnotation
persistent identity: annotation1959540
display id: annotation1959540
version: 1
top level: BBa_J64068
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_J64068/annotation1959540/range1959540/1

https://synbiohub.org/public/igem/BBa_J64068/annotation1959540/range1959540/1
type: Range
persistent identity: range1959540
display id: range1959540
version: 1
direction: direction/forward
top level: BBa_J64068
start: 21
end: 37
orientation: inline

BBa_K1520509
type: ComponentDefinition
persistent identity: BBa K1520509
display id: BBa_K1520509
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PgolTS-golS-PgolB-rbs-tetR-Ter-PtetO-rbs-rfp-Ter-Plac-rbs-tetR-Ter-Pcons2-rbs-lacI-Ter
creator: yue wu
created: 2014-09-22T11:00:00Z
modified: 2015-05-08T01:10:49Z
discontinued: false
dominant: false
group u list: _1902_
m user id: 0
owner id: 21318
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: PgolTS-golS-PgolB-rbs-tetR-Ter-PtetO-rbs-rfp-Plac-rbs-tetR-Ter-Pcons2-rbs-lacI-Ter It is a test of our suicide circuit for gold ions.
mutable notes: None
mutable provenance: PgolTS-golS-PgolB, tetR, PtetO, rfp, Plac, tetR, Pcons2, lacI, Ter
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1520026
component: BBa_K1520506
component: BBa_K1520505
component: BBa_K1520029
sequence annotation: BBa_K1520029
sequence annotation: BBa_K1520506
sequence annotation: BBa_K1520026
sequence annotation: BBa_K1520505
sequence: https://synbiohub.org/public/igem/BBa_K1520509_sequence/1

BBa_K1520026
type: Component
persistent identity: component2385559
display id: component2385559
version: 1
top level: BBa_K1520509
access: public
definition: BBa_K1520026

BBa_K1520506
type: Component
persistent identity: component2385527
display id: component2385527
version: 1
top level: BBa_K1520509
access: public
definition: https://synbiohub.org/public/igem/BBa_K1520506/1

BBa_K1520505
type: Component
persistent identity: component2385530
display id: component2385530
version: 1
top level: BBa_K1520509
access: public
definition: https://synbiohub.org/public/igem/BBa_K1520505/1

BBa_K1520029
type: Component
persistent identity: component2385531
display id: component2385531
version: 1
top level: BBa_K1520509
access: public
definition: https://synbiohub.org/public/igem/BBa_K1520029/1

BBa_K1520029
type: SequenceAnnotation
persistent identity: annotation2385531
display id: annotation2385531
version: 1
top level: BBa_K1520509
location: https://synbiohub.org/public/igem/BBa_K1520509/annotation2385531/range2385531/1
component: BBa_K1520029

https://synbiohub.org/public/igem/BBa_K1520509/annotation2385531/range2385531/1
type: Range
persistent identity: range2385531
display id: range2385531
version: 1
direction: direction/reverse
top level: BBa_K1520509
start: 1473
end: 1601
orientation: inline

BBa_K1520506
type: SequenceAnnotation
persistent identity: annotation2385527
display id: annotation2385527
version: 1
top level: BBa_K1520509
location: https://synbiohub.org/public/igem/BBa_K1520509/annotation2385527/range2385527/1
component: BBa_K1520506

https://synbiohub.org/public/igem/BBa_K1520509/annotation2385527/range2385527/1
type: Range
persistent identity: range2385527
display id: range2385527
version: 1
direction: direction/reverse
top level: BBa_K1520509
start: 1
end: 753
orientation: inline

BBa_K1520026
type: SequenceAnnotation
persistent identity: annotation2385559
display id: annotation2385559
version: 1
top level: BBa_K1520509
location: https://synbiohub.org/public/igem/BBa_K1520509/annotation2385559/range2385559/1
component: BBa_K1520026

https://synbiohub.org/public/igem/BBa_K1520509/annotation2385559/range2385559/1
type: Range
persistent identity: range2385559
display id: range2385559
version: 1
direction: direction/reverse
top level: BBa_K1520509
start: 1610
end: 4955
orientation: inline

BBa_K1520505
type: SequenceAnnotation
persistent identity: annotation2385530
display id: annotation2385530
version: 1
top level: BBa_K1520509
location: https://synbiohub.org/public/igem/BBa_K1520509/annotation2385530/range2385530/1
component: BBa_K1520505

https://synbiohub.org/public/igem/BBa_K1520509/annotation2385530/range2385530/1
type: Range
persistent identity: range2385530
display id: range2385530
version: 1
direction: direction/reverse
top level: BBa_K1520509
start: 762
end: 1464
orientation: inline

BBa_J34809
type: ComponentDefinition
persistent identity: BBa J34809
display id: BBa_J34809
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: promoter lac induced
creator: Alexandra Choutko
created: 2006-10-25T11:00:00Z
modified: 2015-08-31T04:08:47Z
discontinued: false
dominant: false
group u list: _62_
m user id: 0
owner id: 1085
owning group id: 62
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: improved for transcription factor BBa_J34807 binding
mutable notes: a
mutable provenance: a
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J34809_sequence/1

BBa_J61055
type: ComponentDefinition
persistent identity: BBa J61055
display id: BBa_J61055
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: [HIP-1fnr] Promoter
creator: John Anderson
created: 2007-02-20T12:00:00Z
modified: 2015-08-31T02:03:00Z
discontinued: false
dominant: false
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: non
mutable notes: non
mutable provenance: non
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J61055_sequence/1

argB
type: ComponentDefinition
persistent identity: BBa K678043
display id: BBa_K678043
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: argB cassette, selection marker for Aspergillus nidulans
creator: DTU-Denmark-2
created: 2011-09-04T11:00:00Z
modified: 2015-05-08T01:13:04Z
discontinued: false
dominant: false
experience: experience/None
group u list: _882_
m user id: 0
owner id: 8341
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: The Aspergillus nidulans gene argB encodes ornithine transcarbamylase and can be used as an auxotrophic marker in the filamentous fungi A. nidulans and A. niger. The successfull use of argB as a selection marker has been documented and described extensively in the literature.
mutable notes: Compatible with the Plug'n'Play assembly standard
mutable provenance: Aspergillus nidulans genomic DNA
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: Linker 5
sequence annotation: argB cassette
sequence: https://synbiohub.org/public/igem/BBa_K678043_sequence/1

Linker 5
type: SequenceAnnotation
persistent identity: annotation2127417
display id: annotation2127417
version: 1
top level: argB
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K678043/annotation2127417/range2127417/1

https://synbiohub.org/public/igem/BBa_K678043/annotation2127417/range2127417/1
type: Range
persistent identity: range2127417
display id: range2127417
version: 1
direction: direction/forward
top level: argB
start: 1
end: 8
orientation: inline

argB cassette
type: SequenceAnnotation
persistent identity: annotation2127418
display id: annotation2127418
version: 1
top level: argB
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K678043/annotation2127418/range2127418/1

https://synbiohub.org/public/igem/BBa_K678043/annotation2127418/range2127418/1
type: Range
persistent identity: range2127418
display id: range2127418
version: 1
direction: direction/forward
top level: argB
start: 9
end: 2165
orientation: inline

BBa_K110016
type: ComponentDefinition
persistent identity: BBa K110016
display id: BBa_K110016
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: A-Cell Promoter STE2 (backwards)
creator: James DiCarlo
created: 2008-08-26T11:00:00Z
modified: 2015-05-08T01:09:09Z
discontinued: false
dominant: false
experience: experience/None
group u list: _201_
m user id: 0
owner id: 2669
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This is the same sequence as STE2 promotor(BBa_K110008), but with suffix and prefix switched
mutable notes: This is directly from the DNA sequence
mutable provenance: sgd
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: STE12
sequence annotation: MATalpha2
sequence annotation: STE2 Promoter
sequence annotation: MCM1
sequence: https://synbiohub.org/public/igem/BBa_K110016_sequence/1

STE12
type: SequenceAnnotation
persistent identity: annotation1981095
display id: annotation1981095
version: 1
top level: BBa_K110016
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K110016/annotation1981095/range1981095/1

https://synbiohub.org/public/igem/BBa_K110016/annotation1981095/range1981095/1
type: Range
persistent identity: range1981095
display id: range1981095
version: 1
direction: direction/forward
top level: BBa_K110016
start: 304
end: 311
orientation: inline

MATalpha2
type: SequenceAnnotation
persistent identity: annotation1981091
display id: annotation1981091
version: 1
top level: BBa_K110016
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K110016/annotation1981091/range1981091/1

https://synbiohub.org/public/igem/BBa_K110016/annotation1981091/range1981091/1
type: Range
persistent identity: range1981091
display id: range1981091
version: 1
direction: direction/forward
top level: BBa_K110016
start: 273
end: 282
orientation: inline

STE2 Promoter
type: SequenceAnnotation
persistent identity: annotation1981094
display id: annotation1981094
version: 1
top level: BBa_K110016
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K110016/annotation1981094/range1981094/1

https://synbiohub.org/public/igem/BBa_K110016/annotation1981094/range1981094/1
type: Range
persistent identity: range1981094
display id: range1981094
version: 1
direction: direction/forward
top level: BBa_K110016
start: 1
end: 500
orientation: inline

MATalpha2
type: SequenceAnnotation
persistent identity: annotation1981093
display id: annotation1981093
version: 1
top level: BBa_K110016
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K110016/annotation1981093/range1981093/1

https://synbiohub.org/public/igem/BBa_K110016/annotation1981093/range1981093/1
type: Range
persistent identity: range1981093
display id: range1981093
version: 1
direction: direction/forward
top level: BBa_K110016
start: 295
end: 302
orientation: inline

MCM1
type: SequenceAnnotation
persistent identity: annotation1981092
display id: annotation1981092
version: 1
top level: BBa_K110016
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K110016/annotation1981092/range1981092/1

https://synbiohub.org/public/igem/BBa_K110016/annotation1981092/range1981092/1
type: Range
persistent identity: range1981092
display id: range1981092
version: 1
direction: direction/forward
top level: BBa_K110016
start: 284
end: 293
orientation: inline

BBa_K1486042
type: ComponentDefinition
persistent identity: BBa K1486042
display id: BBa_K1486042
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Leucine Zipper
creator: EPFL iGem team 2014
created: 2014-10-01T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: To edit
mutable notes: To edit
mutable provenance: To edit
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence: https://synbiohub.org/public/igem/BBa_K1486042_sequence/1

BBa_B0038
type: ComponentDefinition
persistent identity: BBa B0038
display id: BBa_B0038
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS 1
creator: Josh Michener
created: 2005-12-20T12:00:00Z
modified: 2015-08-31T04:07:20Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_
m user id: 0
owner id: 253
owning group id: 6
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Modified version of B0031 from the Voigt Lab. Not currently available as an independent part (only in a tet inverter).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_B0038_sequence/1

BBa_I756019
type: ComponentDefinition
persistent identity: BBa I756019
display id: BBa_I756019
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Lac Operator in SV-40 intron
creator: Jack
created: 2007-08-15T11:00:00Z
modified: 2015-08-31T04:08:06Z
discontinued: false
dominant: true
group u list: _108_
m user id: 0
owner id: 2067
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Lac Operator in SV-40 intron
mutable notes: Lac Operator in SV-40 intron
mutable provenance: Lac Operator in SV-40 intron
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I756019_sequence/1

BBa_B2022
type: ComponentDefinition
persistent identity: BBa B2022
display id: BBa_B2022
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: 4B RBS from T7
creator: Bartholomew Canton
created: 2007-05-22T11:00:00Z
modified: 2015-08-31T04:07:21Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_
m user id: 0
owner id: 135
owning group id: 84
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: to be added automatically later
mutable notes: to be added automatically later
mutable provenance: to be added automatically later
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_B2022_sequence/1

T7 0.5
type: ComponentDefinition
persistent identity: BBa B2003
display id: BBa_B2003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: T7 RBS 0.5
creator: Heather Keller
created: 2006-09-27T11:00:00Z
modified: 2015-08-31T04:07:21Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_
m user id: 0
owner id: 571
owning group id: 10
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is the -20 to -1 region of T7 gp0.5, which contains the native RBS region for this gene. The physical instantiation of this part does not exist. This part is not compatible with Standard Biobricks assembly methods, as the scar created during assembly would interrupt the spacing between the RBS and the start codon of the CDS. Individuals wishing to use this RBS sequence in their assembles should refer to part B2103.
mutable notes: The -20 to -1 region was chosen to be representative of the RBS region of the T7 genes for the following reasons: 1) The Shine-Delgarno region is contained within this defined region for all T7 genes. 2) Current literature suggests that when bound to the Translation Start Site (0), the Ribosome covers positions -20 to +13 of the gene.
mutable provenance: Genome of wild-type bacteriophage T7, positions 1476-1495. Includes the -20 to -1 region of gp0.5.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: SD region
sequence: https://synbiohub.org/public/igem/BBa_B2003_sequence/1

SD region
type: SequenceAnnotation
persistent identity: annotation1902509
display id: annotation1902509
version: 1
top level: T7 0.5
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_B2003/annotation1902509/range1902509/1

https://synbiohub.org/public/igem/BBa_B2003/annotation1902509/range1902509/1
type: Range
persistent identity: range1902509
display id: range1902509
version: 1
direction: direction/forward
top level: T7 0.5
start: 13
end: 18
orientation: inline

BBa_K088007
type: ComponentDefinition
persistent identity: BBa K088007
display id: BBa_K088007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: GlnRS promoter
creator: Yaguang Liu
created: 2008-10-23T11:00:00Z
modified: 2015-05-08T01:08:37Z
discontinued: false
dominant: false
group u list: _190_
m user id: 0
owner id: 3108
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part promotes the transcription of TyrRS(aminoacyl tRNA synthetase).
mutable notes: It is a weak promoter.
mutable provenance: E.coli We synthetized it according to the GeneBank.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K088007_sequence/1

Pu
type: ComponentDefinition
persistent identity: BBa I723020
display id: BBa_I723020
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Pu
creator: Scott Ramsay
created: 2007-10-24T11:00:00Z
modified: 2015-08-31T04:07:54Z
discontinued: false
dominant: false
experience: experience/None
group u list: _126_
m user id: 0
owner id: 2140
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This is the promoter activated by the transcriptional regulator XylR (BBa_I723136) in response to detection of environmental xylene.
mutable notes: [Christine/Maia]
mutable provenance: [Christine/Maia]
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: Pu
sequence: https://synbiohub.org/public/igem/BBa_I723020_sequence/1

Pu
type: SequenceAnnotation
persistent identity: annotation1955192
display id: annotation1955192
version: 1
top level: Pu
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I723020/annotation1955192/range1955192/1

https://synbiohub.org/public/igem/BBa_I723020/annotation1955192/range1955192/1
type: Range
persistent identity: range1955192
display id: range1955192
version: 1
direction: direction/forward
top level: Pu
start: 1
end: 320
orientation: inline

BBa_R0085
type: ComponentDefinition
persistent identity: BBa R0085
display id: BBa_R0085
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: T7 Consensus Promoter Sequence
creator: Barry Canton
created: 2005-02-21T12:00:00Z
modified: 2015-05-08T01:14:15Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_6_
m user id: 0
owner id: 135
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: The T7 promoter should only produce PoPS when the T7 polymerase is also being expressed.
mutable provenance: Sequence obtained from Sri Kosuri
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: Initiation Region
sequence annotation: Polymerase Binding Region
sequence annotation: Transcription Start Site
sequence: https://synbiohub.org/public/igem/BBa_R0085_sequence/1

Initiation Region
type: SequenceAnnotation
persistent identity: annotation1721522
display id: annotation1721522
version: 1
top level: BBa_R0085
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_R0085/annotation1721522/range1721522/1

https://synbiohub.org/public/igem/BBa_R0085/annotation1721522/range1721522/1
type: Range
persistent identity: range1721522
display id: range1721522
version: 1
direction: direction/forward
top level: BBa_R0085
start: 12
end: 23
orientation: inline

Polymerase Binding Region
type: SequenceAnnotation
persistent identity: annotation1721521
display id: annotation1721521
version: 1
top level: BBa_R0085
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0085/annotation1721521/range1721521/1

https://synbiohub.org/public/igem/BBa_R0085/annotation1721521/range1721521/1
type: Range
persistent identity: range1721521
display id: range1721521
version: 1
direction: direction/forward
top level: BBa_R0085
start: 1
end: 11
orientation: inline

Transcription Start Site
type: SequenceAnnotation
persistent identity: annotation1721520
display id: annotation1721520
version: 1
top level: BBa_R0085
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_R0085/annotation1721520/range1721520/1

https://synbiohub.org/public/igem/BBa_R0085/annotation1721520/range1721520/1
type: Range
persistent identity: range1721520
display id: range1721520
version: 1
direction: direction/forward
top level: BBa_R0085
start: 18
end: 18
orientation: inline

GFP
type: ComponentDefinition
persistent identity: BBa K678027
display id: BBa_K678027
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: GFP for Aspergillus nidulans
creator: DTU-Denmark-2
created: 2011-09-10T11:00:00Z
modified: 2015-05-08T01:13:04Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _882_
m user id: 0
owner id: 8341
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: GFP for Aspergillus nidulans
mutable notes: Design considerations This part was designed for the Plug'n'Play with DNA assembly standard (RFC 80), therefore this part contains linkers at the 5' and 3' ends as shown on the figure below.
mutable provenance: to be announced
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: GFP
sequence annotation: Linker 2
sequence: https://synbiohub.org/public/igem/BBa_K678027_sequence/1

GFP
type: SequenceAnnotation
persistent identity: annotation2127383
display id: annotation2127383
version: 1
top level: GFP
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K678027/annotation2127383/range2127383/1

https://synbiohub.org/public/igem/BBa_K678027/annotation2127383/range2127383/1
type: Range
persistent identity: range2127383
display id: range2127383
version: 1
direction: direction/forward
top level: GFP
start: 9
end: 728
orientation: inline

Linker 2
type: SequenceAnnotation
persistent identity: annotation2127382
display id: annotation2127382
version: 1
top level: GFP
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K678027/annotation2127382/range2127382/1

https://synbiohub.org/public/igem/BBa_K678027/annotation2127382/range2127382/1
type: Range
persistent identity: range2127382
display id: range2127382
version: 1
direction: direction/forward
top level: GFP
start: 1
end: 8
orientation: inline

GFP-TS
type: ComponentDefinition
persistent identity: BBa K678029
display id: BBa_K678029
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: GFP-TS for Aspergillus nidulans
creator: DTU-Denmark-2
created: 2011-09-10T11:00:00Z
modified: 2015-05-08T01:13:04Z
discontinued: false
dominant: false
experience: experience/None
group u list: _882_
m user id: 0
owner id: 8341
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: GFP for Aspergillus nidulans
mutable notes: Design considerations This part was designed for the Plug'n'Play with DNA assembly standard (RFC 80), therefore this part contains linkers at the 5' and 3' ends as shown on the figure below.
mutable provenance: To be announced
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: Linker 3
sequence annotation: GFP
sequence: https://synbiohub.org/public/igem/BBa_K678029_sequence/1

Linker 3
type: SequenceAnnotation
persistent identity: annotation2127386
display id: annotation2127386
version: 1
top level: GFP-TS
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K678029/annotation2127386/range2127386/1

https://synbiohub.org/public/igem/BBa_K678029/annotation2127386/range2127386/1
type: Range
persistent identity: range2127386
display id: range2127386
version: 1
direction: direction/forward
top level: GFP-TS
start: 1
end: 9
orientation: inline

GFP
type: SequenceAnnotation
persistent identity: annotation2127387
display id: annotation2127387
version: 1
top level: GFP-TS
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K678029/annotation2127387/range2127387/1

https://synbiohub.org/public/igem/BBa_K678029/annotation2127387/range2127387/1
type: Range
persistent identity: range2127387
display id: range2127387
version: 1
direction: direction/forward
top level: GFP-TS
start: 10
end: 729
orientation: inline

BBa_J23119
type: ComponentDefinition
persistent identity: BBa J23119
display id: BBa_J23119
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: constitutive promoter family member
creator: John Anderson
created: 2006-08-23T11:00:00Z
modified: 2015-08-31T04:08:40Z
discontinued: false
dominant: true
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Later
mutable notes: N/A
mutable provenance: Overlap extension of synthetic oligonucleotides
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J23119_sequence/1

glnAp2 pro
type: ComponentDefinition
persistent identity: BBa J64979
display id: BBa_J64979
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: glnAp2
creator: Kristopher Kuchenbecker
created: 2007-03-25T12:00:00Z
modified: 2015-05-08T01:08:18Z
discontinued: false
dominant: false
experience: experience/None
group u list: _98_
m user id: 0
owner id: 1429
owning group id: 98
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This promoter sequence (-80 to +1) is responsive to the transcription enhancer glnG.
mutable notes: n/a
mutable provenance: ''E. coli''
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J64979_sequence/1

U037O11+D0
type: ComponentDefinition
persistent identity: BBa I732444
display id: BBa_I732444
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Family Member (U037O11+D014O22)
creator: Zhan Jian
created: 2007-10-13T11:00:00Z
modified: 2015-08-31T04:07:58Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732444_sequence/1

PalcA
type: ComponentDefinition
persistent identity: BBa K678001
display id: BBa_K678001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PalcA, inducible Aspergillus nidulans promoter
creator: DTU-Denmark-2
created: 2011-09-01T11:00:00Z
modified: 2015-05-08T01:13:04Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _882_
m user id: 0
owner id: 8341
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: A promoter that is inducible by alcohol, threonine can be used as an inducer.
mutable notes: Compatible with the Plug'n'Play with DNA assembly standard
mutable provenance: from plasmid p82, CMB strain collection
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: Linker 2
sequence annotation: PalcA
sequence annotation: Linker 1
sequence: https://synbiohub.org/public/igem/BBa_K678001_sequence/1

Linker 2
type: SequenceAnnotation
persistent identity: annotation2125769
display id: annotation2125769
version: 1
top level: PalcA
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K678001/annotation2125769/range2125769/1

https://synbiohub.org/public/igem/BBa_K678001/annotation2125769/range2125769/1
type: Range
persistent identity: range2125769
display id: range2125769
version: 1
direction: direction/forward
top level: PalcA
start: 844
end: 851
orientation: inline

PalcA
type: SequenceAnnotation
persistent identity: annotation2127155
display id: annotation2127155
version: 1
top level: PalcA
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K678001/annotation2127155/range2127155/1

https://synbiohub.org/public/igem/BBa_K678001/annotation2127155/range2127155/1
type: Range
persistent identity: range2127155
display id: range2127155
version: 1
direction: direction/forward
top level: PalcA
start: 9
end: 843
orientation: inline

Linker 1
type: SequenceAnnotation
persistent identity: annotation2127154
display id: annotation2127154
version: 1
top level: PalcA
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K678001/annotation2127154/range2127154/1

https://synbiohub.org/public/igem/BBa_K678001/annotation2127154/range2127154/1
type: Range
persistent identity: range2127154
display id: range2127154
version: 1
direction: direction/forward
top level: PalcA
start: 1
end: 8
orientation: inline

BBa_I741021
type: ComponentDefinition
persistent identity: BBa I741021
display id: BBa_I741021
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: promoter to xylA without CRP and several binding sites for xylR
creator: Lucien Weiss
created: 2007-06-20T11:00:00Z
modified: 2015-08-31T04:08:02Z
discontinued: false
dominant: false
experience: experience/Issues
group u list: _143_
m user id: 0
owner id: 1763
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: removed crp binding site removed rbs removed several xylR binding sites
mutable provenance: ecoli K12 genome
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: promoter
sequence: https://synbiohub.org/public/igem/BBa_I741021_sequence/1

promoter
type: SequenceAnnotation
persistent identity: annotation1935206
display id: annotation1935206
version: 1
top level: BBa_I741021
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I741021/annotation1935206/range1935206/1

https://synbiohub.org/public/igem/BBa_I741021/annotation1935206/range1935206/1
type: Range
persistent identity: range1935206
display id: range1935206
version: 1
direction: direction/reverse
top level: BBa_I741021
start: 15
end: 75
orientation: inline

BBa_B0041
type: ComponentDefinition
persistent identity: BBa B0041
display id: BBa_B0041
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS 3
creator: Josh Michener
created: 2005-12-20T12:00:00Z
modified: 2015-08-31T04:07:20Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_
m user id: 0
owner id: 253
owning group id: 6
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Modified version of B0031 from the Voigt Lab. Not currently available as an independent part (only in a tet inverter).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_B0041_sequence/1

BBa_K1824893
type: ComponentDefinition
persistent identity: BBa K1824893
display id: BBa_K1824893
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: a strong promoter plus a strong RBS
creator: Xinhao Wang
created: 2015-09-09T11:00:00Z
modified: 2015-09-11T08:37:07Z
discontinued: false
dominant: false
group u list: _2250_
m user id: 26000
owner id: 26000
owning group id: 9
status: status/Planning
bookmark: false
mutable description: A regulatory part composed of a strong promoter and a strong RBS, which can be used in both transcription and translation. Just add any protein after it.
mutable notes: No special concern
mutable provenance: J23119 is from a constitutive promoter family and B0034 is based on Elowitz repressilator.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1824890
component: BBa_K1824889
component: BBa_B0034
component: BBa_J23119
sequence annotation: BBa_K1824890
sequence annotation: BBa_J23119
sequence annotation: BBa_K1824889
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K1824893_sequence/1

BBa_K1824890
type: Component
persistent identity: component2450243
display id: component2450243
version: 1
top level: BBa_K1824893
access: public
definition: https://synbiohub.org/public/igem/BBa_K1824890/1

BBa_K1824889
type: Component
persistent identity: component2450240
display id: component2450240
version: 1
top level: BBa_K1824893
access: public
definition: https://synbiohub.org/public/igem/BBa_K1824889/1

BBa_B0034
type: Component
persistent identity: component2450242
display id: component2450242
version: 1
top level: BBa_K1824893
access: public
definition: BBa_B0034

BBa_J23119
type: Component
persistent identity: component2450239
display id: component2450239
version: 1
top level: BBa_K1824893
access: public
definition: BBa_J23119

BBa_K1824890
type: SequenceAnnotation
persistent identity: annotation2450243
display id: annotation2450243
version: 1
top level: BBa_K1824893
location: https://synbiohub.org/public/igem/BBa_K1824893/annotation2450243/range2450243/1
component: BBa_K1824890

https://synbiohub.org/public/igem/BBa_K1824893/annotation2450243/range2450243/1
type: Range
persistent identity: range2450243
display id: range2450243
version: 1
direction: direction/reverse
top level: BBa_K1824893
start: 56
end: 61
orientation: inline

BBa_J23119
type: SequenceAnnotation
persistent identity: annotation2450239
display id: annotation2450239
version: 1
top level: BBa_K1824893
location: https://synbiohub.org/public/igem/BBa_K1824893/annotation2450239/range2450239/1
component: BBa_J23119

https://synbiohub.org/public/igem/BBa_K1824893/annotation2450239/range2450239/1
type: Range
persistent identity: range2450239
display id: range2450239
version: 1
direction: direction/reverse
top level: BBa_K1824893
start: 1
end: 35
orientation: inline

BBa_K1824889
type: SequenceAnnotation
persistent identity: annotation2450240
display id: annotation2450240
version: 1
top level: BBa_K1824893
location: https://synbiohub.org/public/igem/BBa_K1824893/annotation2450240/range2450240/1
component: BBa_K1824889

https://synbiohub.org/public/igem/BBa_K1824893/annotation2450240/range2450240/1
type: Range
persistent identity: range2450240
display id: range2450240
version: 1
direction: direction/reverse
top level: BBa_K1824893
start: 36
end: 43
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2450242
display id: annotation2450242
version: 1
top level: BBa_K1824893
location: https://synbiohub.org/public/igem/BBa_K1824893/annotation2450242/range2450242/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1824893/annotation2450242/range2450242/1
type: Range
persistent identity: range2450242
display id: range2450242
version: 1
direction: direction/reverse
top level: BBa_K1824893
start: 44
end: 55
orientation: inline

BBa_J13210
type: ComponentDefinition
persistent identity: BBa J13210
display id: BBa_J13210
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pOmpR dependent POPS producer
creator: Jeff Tabor
created: 2005-07-18T11:00:00Z
modified: 2015-08-31T04:08:30Z
discontinued: false
dominant: false
experience: experience/None
group u list: _37_
m user id: 0
owner id: 88
owning group id: 37
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
component: BBa_B0012
component: BBa_B0010
component: BBa_R0082
sequence annotation: BBa_R0082
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_J13210_sequence/1

BBa_B0012
type: Component
persistent identity: component1587097
display id: component1587097
version: 1
top level: BBa_J13210
access: public
definition: BBa_B0012

BBa_B0010
type: Component
persistent identity: component1587087
display id: component1587087
version: 1
top level: BBa_J13210
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_R0082
type: Component
persistent identity: component1587117
display id: component1587117
version: 1
top level: BBa_J13210
access: public
definition: OmpR

BBa_R0082
type: SequenceAnnotation
persistent identity: annotation1587117
display id: annotation1587117
version: 1
top level: BBa_J13210
location: https://synbiohub.org/public/igem/BBa_J13210/annotation1587117/range1587117/1
component: BBa_R0082

https://synbiohub.org/public/igem/BBa_J13210/annotation1587117/range1587117/1
type: Range
persistent identity: range1587117
display id: range1587117
version: 1
direction: direction/forward
top level: BBa_J13210
start: 138
end: 245
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1587087
display id: annotation1587087
version: 1
top level: BBa_J13210
location: https://synbiohub.org/public/igem/BBa_J13210/annotation1587087/range1587087/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_J13210/annotation1587087/range1587087/1
type: Range
persistent identity: range1587087
display id: range1587087
version: 1
direction: direction/forward
top level: BBa_J13210
start: 1
end: 80
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1587097
display id: annotation1587097
version: 1
top level: BBa_J13210
location: https://synbiohub.org/public/igem/BBa_J13210/annotation1587097/range1587097/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_J13210/annotation1587097/range1587097/1
type: Range
persistent identity: range1587097
display id: range1587097
version: 1
direction: direction/forward
top level: BBa_J13210
start: 89
end: 129
orientation: inline

BBa_J23107
type: ComponentDefinition
persistent identity: BBa J23107
display id: BBa_J23107
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: constitutive promoter family member
creator: John Anderson
created: 2006-08-16T11:00:00Z
modified: 2015-08-31T04:08:40Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: Later
mutable notes: N/A
mutable provenance: Later
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J23107_sequence/1

BBa_K1520026
type: ComponentDefinition
persistent identity: BBa K1520026
display id: BBa_K1520026
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Ptet-rbs-rfp-Ter-Plac-rbs-tetR-Ter-Pcons2-rbs-lacI-Ter
creator: yue wu
created: 2014-09-21T11:00:00Z
modified: 2015-05-08T01:10:49Z
discontinued: false
dominant: false
group u list: _1902_
m user id: 0
owner id: 21318
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Ptet-rbs-rfp-Ter-Plac-rbs-tetR-Ter-Pcons2-rbs-lacI-Ter. It produces rfp when there is less lactose.
mutable notes: None
mutable provenance: Ptet, rfp, Plac, tetR, Pcons2, lacI, Ter
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1520008
component: BBa_K1520025
sequence annotation: BBa_K1520025
sequence annotation: BBa_K1520008
sequence: https://synbiohub.org/public/igem/BBa_K1520026_sequence/1

BBa_K1520008
type: Component
persistent identity: component2385319
display id: component2385319
version: 1
top level: BBa_K1520026
access: public
definition: https://synbiohub.org/public/igem/BBa_K1520008/1

BBa_K1520025
type: Component
persistent identity: component2385313
display id: component2385313
version: 1
top level: BBa_K1520026
access: public
definition: BBa_K1520025

BBa_K1520025
type: SequenceAnnotation
persistent identity: annotation2385313
display id: annotation2385313
version: 1
top level: BBa_K1520026
location: https://synbiohub.org/public/igem/BBa_K1520026/annotation2385313/range2385313/1
component: BBa_K1520025

https://synbiohub.org/public/igem/BBa_K1520026/annotation2385313/range2385313/1
type: Range
persistent identity: range2385313
display id: range2385313
version: 1
direction: direction/reverse
top level: BBa_K1520026
start: 1
end: 1987
orientation: inline

BBa_K1520008
type: SequenceAnnotation
persistent identity: annotation2385319
display id: annotation2385319
version: 1
top level: BBa_K1520026
location: https://synbiohub.org/public/igem/BBa_K1520026/annotation2385319/range2385319/1
component: BBa_K1520008

https://synbiohub.org/public/igem/BBa_K1520026/annotation2385319/range2385319/1
type: Range
persistent identity: range2385319
display id: range2385319
version: 1
direction: direction/reverse
top level: BBa_K1520026
start: 1996
end: 3346
orientation: inline

BBa_J64069
type: ComponentDefinition
persistent identity: BBa J64069
display id: BBa_J64069
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: R0065 with lux box deleted
creator: Jeffrey J Tabor
created: 2008-02-10T12:00:00Z
modified: 2015-05-08T01:08:17Z
discontinued: false
dominant: false
experience: experience/None
group u list: _98_
m user id: 0
owner id: 88
owning group id: 98
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: luxR operator (lux box) was deleted from R0065 and replaced with a BamHI hexamer.
mutable notes: added a bamHI site immediately upstream of -35 in place of lux operator
mutable provenance: constructed
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: +1
sequence annotation: -10
sequence annotation: -35
sequence: https://synbiohub.org/public/igem/BBa_J64069_sequence/1

+1
type: SequenceAnnotation
persistent identity: annotation1961162
display id: annotation1961162
version: 1
top level: BBa_J64069
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_J64069/annotation1961162/range1961162/1

https://synbiohub.org/public/igem/BBa_J64069/annotation1961162/range1961162/1
type: Range
persistent identity: range1961162
display id: range1961162
version: 1
direction: direction/forward
top level: BBa_J64069
start: 46
end: 46
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1961161
display id: annotation1961161
version: 1
top level: BBa_J64069
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_J64069/annotation1961161/range1961161/1

https://synbiohub.org/public/igem/BBa_J64069/annotation1961161/range1961161/1
type: Range
persistent identity: range1961161
display id: range1961161
version: 1
direction: direction/forward
top level: BBa_J64069
start: 34
end: 39
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1961160
display id: annotation1961160
version: 1
top level: BBa_J64069
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_J64069/annotation1961160/range1961160/1

https://synbiohub.org/public/igem/BBa_J64069/annotation1961160/range1961160/1
type: Range
persistent identity: range1961160
display id: range1961160
version: 1
direction: direction/forward
top level: BBa_J64069
start: 12
end: 17
orientation: inline

BBa_K101001
type: ComponentDefinition
persistent identity: BBa K101001
display id: BBa_K101001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Dual-Repressed Promoter for LacI and LambdacI
creator: Bennett Swiniarski
created: 2008-07-27T11:00:00Z
modified: 2015-05-08T01:08:41Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _205_
m user id: 0
owner id: 3363
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This part is a new construct of a promoter that is dually repressed by either LacI or LambdacI proteins.
mutable notes: LambdacI usually binds to two operator sites, one located upstream from -35 and one located between -35 and -10. By itself, LacI binds downstream from -10. This made the part design very straightforward, since the locations of the operator sites do not overlap at all. In order to complete this part, we simply combined the proper sequences and we had a dually-repressed promoter.
mutable provenance: This part was made by combining BioBrick parts R0010(LacI promoter) and R0051 (LambdacI promoter), along with intermediate sequence between these operator sites.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K101001_sequence/1

pAND
type: ComponentDefinition
persistent identity: BBa J58100
display id: BBa_J58100
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: AND-type promoter synergistically activated by cI and CRP
creator: Valencia iGEM 2006
created: 2006-10-25T11:00:00Z
modified: 2015-08-31T03:14:48Z
discontinued: false
dominant: false
experience: experience/None
group u list: _83_
m user id: 0
owner id: 1100
owning group id: 83
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: none
mutable notes: none
mutable provenance: none
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: CRP binding site
sequence annotation: spacer from I12007 (77-82)
sequence annotation: spacer from I12007 (26-32)
sequence annotation: -10
sequence annotation: lam-cI OR2 binding site
sequence annotation: -35
sequence annotation: spacer from I12007 (54-70)
sequence annotation: spacer from I12007 (1-11, 59-72)
sequence: https://synbiohub.org/public/igem/BBa_J58100_sequence/1

CRP binding site
type: SequenceAnnotation
persistent identity: annotation1908168
display id: annotation1908168
version: 1
top level: pAND
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_J58100/annotation1908168/range1908168/1

https://synbiohub.org/public/igem/BBa_J58100/annotation1908168/range1908168/1
type: Range
persistent identity: range1908168
display id: range1908168
version: 1
direction: direction/forward
top level: pAND
start: 3
end: 24
orientation: inline

spacer from I12007 (77-82)
type: SequenceAnnotation
persistent identity: annotation1908182
display id: annotation1908182
version: 1
top level: pAND
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J58100/annotation1908182/range1908182/1

https://synbiohub.org/public/igem/BBa_J58100/annotation1908182/range1908182/1
type: Range
persistent identity: range1908182
display id: range1908182
version: 1
direction: direction/forward
top level: pAND
start: 101
end: 106
orientation: inline

spacer from I12007 (26-32)
type: SequenceAnnotation
persistent identity: annotation1908180
display id: annotation1908180
version: 1
top level: pAND
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J58100/annotation1908180/range1908180/1

https://synbiohub.org/public/igem/BBa_J58100/annotation1908180/range1908180/1
type: Range
persistent identity: range1908180
display id: range1908180
version: 1
direction: direction/forward
top level: pAND
start: 50
end: 56
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1908179
display id: annotation1908179
version: 1
top level: pAND
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_J58100/annotation1908179/range1908179/1

https://synbiohub.org/public/igem/BBa_J58100/annotation1908179/range1908179/1
type: Range
persistent identity: range1908179
display id: range1908179
version: 1
direction: direction/forward
top level: pAND
start: 95
end: 100
orientation: inline

lam-cI OR2 binding site
type: SequenceAnnotation
persistent identity: annotation1908169
display id: annotation1908169
version: 1
top level: pAND
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_J58100/annotation1908169/range1908169/1

https://synbiohub.org/public/igem/BBa_J58100/annotation1908169/range1908169/1
type: Range
persistent identity: range1908169
display id: range1908169
version: 1
direction: direction/forward
top level: pAND
start: 57
end: 74
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1908178
display id: annotation1908178
version: 1
top level: pAND
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_J58100/annotation1908178/range1908178/1

https://synbiohub.org/public/igem/BBa_J58100/annotation1908178/range1908178/1
type: Range
persistent identity: range1908178
display id: range1908178
version: 1
direction: direction/forward
top level: pAND
start: 72
end: 77
orientation: inline

spacer from I12007 (54-70)
type: SequenceAnnotation
persistent identity: annotation1908181
display id: annotation1908181
version: 1
top level: pAND
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J58100/annotation1908181/range1908181/1

https://synbiohub.org/public/igem/BBa_J58100/annotation1908181/range1908181/1
type: Range
persistent identity: range1908181
display id: range1908181
version: 1
direction: direction/forward
top level: pAND
start: 78
end: 94
orientation: inline

spacer from I12007 (1-11, 59-72)
type: SequenceAnnotation
persistent identity: annotation1908171
display id: annotation1908171
version: 1
top level: pAND
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J58100/annotation1908171/range1908171/1

https://synbiohub.org/public/igem/BBa_J58100/annotation1908171/range1908171/1
type: Range
persistent identity: range1908171
display id: range1908171
version: 1
direction: direction/forward
top level: pAND
start: 25
end: 49
orientation: inline

BBa_I12036
type: ComponentDefinition
persistent identity: BBa I12036
display id: BBa_I12036
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Modified lamdba Prm promoter (cooperative repression by 434 cI)
creator: Hans
created: 2004-08-17T11:00:00Z
modified: 2015-08-31T04:07:31Z
discontinued: false
dominant: false
experience: experience/None
group u list: _3_
m user id: 0
owner id: 103
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Lamdba Prm promoter modified to be activated by lamda repressor (cI) and repressed by 434 repressor (cI). Two operators are used for both activation and repression to enhance cooperativity.
mutable notes: The O-R3 site of lambda (17 nt) was replaced by the O-R1 site of 434 (14 nt) to facilitate repression by 434 cI. The new 434 O-R1 is 5'-justified with the lambda O-R3 it replaced, leaving the -10 sequence intact. The 434 O-R2 site replaces the wildtype labmda promoter starting at -5, thereby preserving the 8 nt between 434 O-R1 and O-R2 in the wildtype. The mRNA transcript will contain an additional 9 nt compared to wildtype lambda phage.
mutable provenance: Bushman, F. D. The bacteriophage 434 right operator roles of O-R1, O-R2, and O-R3. J. Mol. Biol. (1993) 230, 28-40.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: start
sequence annotation: -35
sequence annotation: OR2 lambda
sequence annotation: -10
sequence annotation: OR2 434
sequence annotation: OR1 lambda
sequence annotation: OR1 434
sequence: https://synbiohub.org/public/igem/BBa_I12036_sequence/1

start
type: SequenceAnnotation
persistent identity: annotation1062203
display id: annotation1062203
version: 1
top level: BBa_I12036
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_I12036/annotation1062203/range1062203/1

https://synbiohub.org/public/igem/BBa_I12036/annotation1062203/range1062203/1
type: Range
persistent identity: range1062203
display id: range1062203
version: 1
direction: direction/forward
top level: BBa_I12036
start: 83
end: 83
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1056739
display id: annotation1056739
version: 1
top level: BBa_I12036
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I12036/annotation1056739/range1056739/1

https://synbiohub.org/public/igem/BBa_I12036/annotation1056739/range1056739/1
type: Range
persistent identity: range1056739
display id: range1056739
version: 1
direction: direction/forward
top level: BBa_I12036
start: 48
end: 53
orientation: inline

OR2 lambda
type: SequenceAnnotation
persistent identity: annotation1056736
display id: annotation1056736
version: 1
top level: BBa_I12036
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I12036/annotation1056736/range1056736/1

https://synbiohub.org/public/igem/BBa_I12036/annotation1056736/range1056736/1
type: Range
persistent identity: range1056736
display id: range1056736
version: 1
direction: direction/forward
top level: BBa_I12036
start: 33
end: 49
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1056740
display id: annotation1056740
version: 1
top level: BBa_I12036
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I12036/annotation1056740/range1056740/1

https://synbiohub.org/public/igem/BBa_I12036/annotation1056740/range1056740/1
type: Range
persistent identity: range1056740
display id: range1056740
version: 1
direction: direction/forward
top level: BBa_I12036
start: 71
end: 76
orientation: inline

OR2 434
type: SequenceAnnotation
persistent identity: annotation1056738
display id: annotation1056738
version: 1
top level: BBa_I12036
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I12036/annotation1056738/range1056738/1

https://synbiohub.org/public/igem/BBa_I12036/annotation1056738/range1056738/1
type: Range
persistent identity: range1056738
display id: range1056738
version: 1
direction: direction/forward
top level: BBa_I12036
start: 78
end: 91
orientation: inline

OR1 lambda
type: SequenceAnnotation
persistent identity: annotation1056735
display id: annotation1056735
version: 1
top level: BBa_I12036
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I12036/annotation1056735/range1056735/1

https://synbiohub.org/public/igem/BBa_I12036/annotation1056735/range1056735/1
type: Range
persistent identity: range1056735
display id: range1056735
version: 1
direction: direction/forward
top level: BBa_I12036
start: 9
end: 25
orientation: inline

OR1 434
type: SequenceAnnotation
persistent identity: annotation1056737
display id: annotation1056737
version: 1
top level: BBa_I12036
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I12036/annotation1056737/range1056737/1

https://synbiohub.org/public/igem/BBa_I12036/annotation1056737/range1056737/1
type: Range
persistent identity: range1056737
display id: range1056737
version: 1
direction: direction/forward
top level: BBa_I12036
start: 56
end: 69
orientation: inline

BBa_M31201
type: ComponentDefinition
persistent identity: BBa M31201
display id: BBa_M31201
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Yeast CLB1 promoter region, G2/M cell cycle specific
creator: Katherine Aull
created: 2008-05-13T11:00:00Z
modified: 2015-05-08T01:13:59Z
discontinued: false
dominant: false
group u list: _89_
m user id: 0
owner id: 624
owning group id: 89
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Description of CLB1 (from SGD): B-type cyclin involved in cell cycle progression; activates Cdc28p to promote the transition from G2 to M phase; accumulates during G2 and M, then targeted via a destruction box motif for ubiquitin-mediated degradation by the proteasome. More information found here: http://db.yeastgenome.org/cgi-bin/locus.pl?locus=CLB1
mutable notes: Part contains 500 bases upstream from the CLB1 ORF. Is NOT a minimal promoter, and has not been tested for composability.
mutable provenance: Yeast consensus sequence from SGD chrVII:703140..703639
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_M31201_sequence/1

U037O11+D0
type: ComponentDefinition
persistent identity: BBa I732406
display id: BBa_I732406
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Family Member (U037O11+D062NUL)
creator: Zhan Jian
created: 2007-09-19T11:00:00Z
modified: 2015-08-31T04:07:58Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1572
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: A Double Reporter (LacZ-alpha and GFP-AAV) loaded on pSB3K5. For more information please refer to BBa_I732403.
mutable notes: Might be later edited.
mutable provenance: Might be later edited.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: lacZ
sequence annotation: stop
sequence annotation: polya
sequence annotation: BBa_E0040
sequence annotation: BamHI
sequence annotation: conserved
sequence annotation: GFP protein
sequence annotation: BBa_B0010
sequence annotation: BBa_I732006
sequence annotation: START
sequence annotation: T7 TE
sequence annotation: BBa_B0034
sequence annotation: STOP
sequence annotation: stem_loop
sequence annotation: BBa_I732007
sequence annotation: BBa_B0012
sequence annotation: BglII
sequence: https://synbiohub.org/public/igem/BBa_I732406_sequence/1

lacZ
type: SequenceAnnotation
persistent identity: annotation1944466
display id: annotation1944466
version: 1
top level: U037O11+D0
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_I732406/annotation1944466/range1944466/1

https://synbiohub.org/public/igem/BBa_I732406/annotation1944466/range1944466/1
type: Range
persistent identity: range1944466
display id: range1944466
version: 1
direction: direction/forward
top level: U037O11+D0
start: 46
end: 279
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1944478
display id: annotation1944478
version: 1
top level: U037O11+D0
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_I732406/annotation1944478/range1944478/1

https://synbiohub.org/public/igem/BBa_I732406/annotation1944478/range1944478/1
type: Range
persistent identity: range1944478
display id: range1944478
version: 1
direction: direction/forward
top level: U037O11+D0
start: 1155
end: 1155
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1944477
display id: annotation1944477
version: 1
top level: U037O11+D0
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_I732406/annotation1944477/range1944477/1

https://synbiohub.org/public/igem/BBa_I732406/annotation1944477/range1944477/1
type: Range
persistent identity: range1944477
display id: range1944477
version: 1
direction: direction/forward
top level: U037O11+D0
start: 1149
end: 1162
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation1944472
display id: annotation1944472
version: 1
top level: U037O11+D0
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_I732406/annotation1944472/range1944472/1

https://synbiohub.org/public/igem/BBa_I732406/annotation1944472/range1944472/1
type: Range
persistent identity: range1944472
display id: range1944472
version: 1
direction: direction/reverse
top level: U037O11+D0
start: 306
end: 1025
orientation: inline

BamHI
type: SequenceAnnotation
persistent identity: annotation1944461
display id: annotation1944461
version: 1
top level: U037O11+D0
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I732406/annotation1944461/range1944461/1

https://synbiohub.org/public/igem/BBa_I732406/annotation1944461/range1944461/1
type: Range
persistent identity: range1944461
display id: range1944461
version: 1
direction: direction/forward
top level: U037O11+D0
start: 12
end: 17
orientation: inline

conserved
type: SequenceAnnotation
persistent identity: annotation1944463
display id: annotation1944463
version: 1
top level: U037O11+D0
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_I732406/annotation1944463/range1944463/1

https://synbiohub.org/public/igem/BBa_I732406/annotation1944463/range1944463/1
type: Range
persistent identity: range1944463
display id: range1944463
version: 1
direction: direction/forward
top level: U037O11+D0
start: 32
end: 35
orientation: inline

GFP protein
type: SequenceAnnotation
persistent identity: annotation1944471
display id: annotation1944471
version: 1
top level: U037O11+D0
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_I732406/annotation1944471/range1944471/1

https://synbiohub.org/public/igem/BBa_I732406/annotation1944471/range1944471/1
type: Range
persistent identity: range1944471
display id: range1944471
version: 1
direction: direction/forward
top level: U037O11+D0
start: 306
end: 1025
orientation: inline

conserved
type: SequenceAnnotation
persistent identity: annotation1944469
display id: annotation1944469
version: 1
top level: U037O11+D0
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_I732406/annotation1944469/range1944469/1

https://synbiohub.org/public/igem/BBa_I732406/annotation1944469/range1944469/1
type: Range
persistent identity: range1944469
display id: range1944469
version: 1
direction: direction/forward
top level: U037O11+D0
start: 292
end: 295
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1944473
display id: annotation1944473
version: 1
top level: U037O11+D0
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_I732406/annotation1944473/range1944473/1

https://synbiohub.org/public/igem/BBa_I732406/annotation1944473/range1944473/1
type: Range
persistent identity: range1944473
display id: range1944473
version: 1
direction: direction/forward
top level: U037O11+D0
start: 1034
end: 1113
orientation: inline

BBa_I732006
type: SequenceAnnotation
persistent identity: annotation1944468
display id: annotation1944468
version: 1
top level: U037O11+D0
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_I732406/annotation1944468/range1944468/1

https://synbiohub.org/public/igem/BBa_I732406/annotation1944468/range1944468/1
type: Range
persistent identity: range1944468
display id: range1944468
version: 1
direction: direction/reverse
top level: U037O11+D0
start: 46
end: 279
orientation: inline

START
type: SequenceAnnotation
persistent identity: annotation1944465
display id: annotation1944465
version: 1
top level: U037O11+D0
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_I732406/annotation1944465/range1944465/1

https://synbiohub.org/public/igem/BBa_I732406/annotation1944465/range1944465/1
type: Range
persistent identity: range1944465
display id: range1944465
version: 1
direction: direction/forward
top level: U037O11+D0
start: 46
end: 48
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1944476
display id: annotation1944476
version: 1
top level: U037O11+D0
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_I732406/annotation1944476/range1944476/1

https://synbiohub.org/public/igem/BBa_I732406/annotation1944476/range1944476/1
type: Range
persistent identity: range1944476
display id: range1944476
version: 1
direction: direction/forward
top level: U037O11+D0
start: 1129
end: 1148
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1944464
display id: annotation1944464
version: 1
top level: U037O11+D0
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_I732406/annotation1944464/range1944464/1

https://synbiohub.org/public/igem/BBa_I732406/annotation1944464/range1944464/1
type: Range
persistent identity: range1944464
display id: range1944464
version: 1
direction: direction/reverse
top level: U037O11+D0
start: 28
end: 39
orientation: inline

STOP
type: SequenceAnnotation
persistent identity: annotation1944467
display id: annotation1944467
version: 1
top level: U037O11+D0
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_I732406/annotation1944467/range1944467/1

https://synbiohub.org/public/igem/BBa_I732406/annotation1944467/range1944467/1
type: Range
persistent identity: range1944467
display id: range1944467
version: 1
direction: direction/forward
top level: U037O11+D0
start: 274
end: 279
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation1944474
display id: annotation1944474
version: 1
top level: U037O11+D0
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_I732406/annotation1944474/range1944474/1

https://synbiohub.org/public/igem/BBa_I732406/annotation1944474/range1944474/1
type: Range
persistent identity: range1944474
display id: range1944474
version: 1
direction: direction/forward
top level: U037O11+D0
start: 1045
end: 1088
orientation: inline

BBa_I732007
type: SequenceAnnotation
persistent identity: annotation1944462
display id: annotation1944462
version: 1
top level: U037O11+D0
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_I732406/annotation1944462/range1944462/1

https://synbiohub.org/public/igem/BBa_I732406/annotation1944462/range1944462/1
type: Range
persistent identity: range1944462
display id: range1944462
version: 1
direction: direction/reverse
top level: U037O11+D0
start: 1
end: 19
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1944475
display id: annotation1944475
version: 1
top level: U037O11+D0
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_I732406/annotation1944475/range1944475/1

https://synbiohub.org/public/igem/BBa_I732406/annotation1944475/range1944475/1
type: Range
persistent identity: range1944475
display id: range1944475
version: 1
direction: direction/forward
top level: U037O11+D0
start: 1122
end: 1162
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1944470
display id: annotation1944470
version: 1
top level: U037O11+D0
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_I732406/annotation1944470/range1944470/1

https://synbiohub.org/public/igem/BBa_I732406/annotation1944470/range1944470/1
type: Range
persistent identity: range1944470
display id: range1944470
version: 1
direction: direction/reverse
top level: U037O11+D0
start: 288
end: 299
orientation: inline

BglII
type: SequenceAnnotation
persistent identity: annotation1944460
display id: annotation1944460
version: 1
top level: U037O11+D0
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I732406/annotation1944460/range1944460/1

https://synbiohub.org/public/igem/BBa_I732406/annotation1944460/range1944460/1
type: Range
persistent identity: range1944460
display id: range1944460
version: 1
direction: direction/forward
top level: U037O11+D0
start: 3
end: 8
orientation: inline

BBa_M13106
type: ComponentDefinition
persistent identity: BBa M13106
display id: BBa_M13106
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: M13K07 gene VI promoter
creator: Natalie Kuldell
created: 2006-12-15T12:00:00Z
modified: 2015-05-08T01:13:55Z
discontinued: false
dominant: false
group u list: _45_
m user id: 0
owner id: 314
owning group id: 1
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This part is from the bacteriophage M13 genome, bp 2716-2764 in M13K07. It directs transcription of M13 gene VI (BBa_M13506, BBa_M13006). Its identity as a promoter was described in Gene (1980) 11:129-148 based on -10,-35 homology and fragment of genome able to bind RNAP.
mutable notes: none
mutable provenance: New England Biolabs M13K07. Sequenced in 2002 by L. Panganaban.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_M13106_sequence/1

BBa_S04509
type: ComponentDefinition
persistent identity: BBa S04509
display id: BBa_S04509
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: R0010:B0034
creator: Jacky, Fong Chuen, Loo
created: 2010-10-19T11:00:00Z
modified: 2015-05-08T01:14:41Z
discontinued: false
dominant: false
group u list: _9_
m user id: 0
owner id: 6123
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_R0010
component: BBa_B0034
sequence annotation: BBa_R0010
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_S04509_sequence/1

BBa_R0010
type: Component
persistent identity: component2090735
display id: component2090735
version: 1
top level: BBa_S04509
access: public
definition: LacI

BBa_B0034
type: Component
persistent identity: component2090743
display id: component2090743
version: 1
top level: BBa_S04509
access: public
definition: BBa_B0034

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation2090735
display id: annotation2090735
version: 1
top level: BBa_S04509
location: https://synbiohub.org/public/igem/BBa_S04509/annotation2090735/range2090735/1
component: BBa_R0010

https://synbiohub.org/public/igem/BBa_S04509/annotation2090735/range2090735/1
type: Range
persistent identity: range2090735
display id: range2090735
version: 1
direction: direction/forward
top level: BBa_S04509
start: 1
end: 200
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2090743
display id: annotation2090743
version: 1
top level: BBa_S04509
location: https://synbiohub.org/public/igem/BBa_S04509/annotation2090743/range2090743/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_S04509/annotation2090743/range2090743/1
type: Range
persistent identity: range2090743
display id: range2090743
version: 1
direction: direction/reverse
top level: BBa_S04509
start: 209
end: 220
orientation: inline

BBa_K112149
type: ComponentDefinition
persistent identity: BBa K112149
display id: BBa_K112149
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PmgtCB Magnesium promoter from Salmonella
creator: Madhvi Venkatesh
created: 2008-11-01T12:00:00Z
modified: 2015-05-08T01:09:14Z
discontinued: false
dominant: false
experience: experience/None
group u list: _224_
m user id: 0
owner id: 3023
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: PmgtCB magnesium promoter
mutable notes: This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at: BBb Standard Description Page
mutable provenance: genome
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K112149_sequence/1

BBa_I756017
type: ComponentDefinition
persistent identity: BBa I756017
display id: BBa_I756017
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: U6 promoter with tet operators
creator: Jack
created: 2007-08-15T11:00:00Z
modified: 2015-08-31T04:08:06Z
discontinued: false
dominant: false
group u list: _108_
m user id: 0
owner id: 2067
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: U6 promoter with tet operators
mutable notes: U6 promoter with tet operators
mutable provenance: U6 promoter with tet operators
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I756017_sequence/1

BBa_K1486046
type: ComponentDefinition
persistent identity: BBa K1486046
display id: BBa_K1486046
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CpxR promoter FW
creator: EPFL iGem team 2014
created: 2014-10-06T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1866_
m user id: 0
owner id: 21033
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Test
mutable notes: Test
mutable provenance: Test
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K1486046_sequence/1

BBa_R2111
type: ComponentDefinition
persistent identity: BBa R2111
display id: BBa_R2111
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter with operator site for C2003
creator: Reshma Shetty
created: 2006-10-20T11:00:00Z
modified: 2015-05-08T01:14:16Z
discontinued: false
dominant: false
group u list: _41_
m user id: 0
owner id: 126
owning group id: 70
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This promoter is designed to bind to C2003.
mutable notes: The placement of the operator was designed to be near optimal for steric interference between C2003 and RNA polymerase.
mutable provenance: This promoter was rationally designed.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: C2003 operator site
sequence annotation: transcription start site
sequence annotation: -10 site
sequence annotation: -35 site
sequence: https://synbiohub.org/public/igem/BBa_R2111_sequence/1

C2003 operator site
type: SequenceAnnotation
persistent identity: annotation1903923
display id: annotation1903923
version: 1
top level: BBa_R2111
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_R2111/annotation1903923/range1903923/1

https://synbiohub.org/public/igem/BBa_R2111/annotation1903923/range1903923/1
type: Range
persistent identity: range1903923
display id: range1903923
version: 1
direction: direction/forward
top level: BBa_R2111
start: 23
end: 34
orientation: inline

transcription start site
type: SequenceAnnotation
persistent identity: annotation1903922
display id: annotation1903922
version: 1
top level: BBa_R2111
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_R2111/annotation1903922/range1903922/1

https://synbiohub.org/public/igem/BBa_R2111/annotation1903922/range1903922/1
type: Range
persistent identity: range1903922
display id: range1903922
version: 1
direction: direction/forward
top level: BBa_R2111
start: 53
end: 53
orientation: inline

-10 site
type: SequenceAnnotation
persistent identity: annotation1903921
display id: annotation1903921
version: 1
top level: BBa_R2111
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R2111/annotation1903921/range1903921/1

https://synbiohub.org/public/igem/BBa_R2111/annotation1903921/range1903921/1
type: Range
persistent identity: range1903921
display id: range1903921
version: 1
direction: direction/forward
top level: BBa_R2111
start: 40
end: 45
orientation: inline

-35 site
type: SequenceAnnotation
persistent identity: annotation1903920
display id: annotation1903920
version: 1
top level: BBa_R2111
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R2111/annotation1903920/range1903920/1

https://synbiohub.org/public/igem/BBa_R2111/annotation1903920/range1903920/1
type: Range
persistent identity: range1903920
display id: range1903920
version: 1
direction: direction/forward
top level: BBa_R2111
start: 17
end: 22
orientation: inline

kanR
type: ComponentDefinition
persistent identity: BBa P1003
display id: BBa_P1003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: kanamycin resistance cassette
creator: Reshma Shetty
created: 2006-01-23T12:00:00Z
modified: 2015-05-08T01:14:11Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _41_6_
m user id: 0
owner id: 126
owning group id: 45
sample status: In stock
status: status/Available
bookmark: false
mutable description: Kanamycin resistance cassette for modular BioBricks vector construction.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: kanamycin resistance
sequence: https://synbiohub.org/public/igem/BBa_P1003_sequence/1

kanamycin resistance
type: SequenceAnnotation
persistent identity: annotation1897112
display id: annotation1897112
version: 1
top level: kanR
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_P1003/annotation1897112/range1897112/1

https://synbiohub.org/public/igem/BBa_P1003/annotation1897112/range1897112/1
type: Range
persistent identity: range1897112
display id: range1897112
version: 1
direction: direction/forward
top level: kanR
start: 149
end: 967
orientation: inline

U073O11+D0
type: ComponentDefinition
persistent identity: BBa I732427
display id: BBa_I732427
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Family Member (U073O11+D026O22)
creator: Zhan Jian
created: 2007-10-13T11:00:00Z
modified: 2015-08-31T04:07:58Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732427_sequence/1

BBa_K1486056
type: ComponentDefinition
persistent identity: BBa K1486056
display id: BBa_K1486056
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CxpR & Split IFP1.4 [Cterm + Cterm][2]
creator: iGem EPFL 2014
created: 2014-10-06T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This part is the twin of the non-compatible biobrick BBa_K1486008 rendered compatible by mutating the two illegal restriction sites. However, all experiments were run on the aforementioned biobrick.
mutable notes: Site-directed mutagenesis was used to remove the restriction sites in the sequence.
mutable provenance: http://www.addgene.org/browse/article/8177/ which was then mutated by us.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_K1486004
component: BBa_K1486000
component: BBa_K1486045
component: BBa_B0034
component: BBa_I0500
component: BBa_K1486044
sequence annotation: BBa_K1486004
sequence annotation: BBa_B0034
sequence annotation: BBa_K1486044
sequence annotation: BBa_I0500
sequence annotation: BBa_K1486045
sequence annotation: BBa_K1486000
sequence: https://synbiohub.org/public/igem/BBa_K1486056_sequence/1

BBa_K1486004
type: Component
persistent identity: component2398614
display id: component2398614
version: 1
top level: BBa_K1486056
access: public
definition: BBa_K1486004

BBa_K1486000
type: Component
persistent identity: component2398613
display id: component2398613
version: 1
top level: BBa_K1486056
access: public
definition: BBa_K1486000

BBa_K1486045
type: Component
persistent identity: component2398615
display id: component2398615
version: 1
top level: BBa_K1486056
access: public
definition: BBa_K1486045

BBa_B0034
type: Component
persistent identity: component2398601
display id: component2398601
version: 1
top level: BBa_K1486056
access: public
definition: BBa_B0034

BBa_B0034
type: Component
persistent identity: component2398609
display id: component2398609
version: 1
top level: BBa_K1486056
access: public
definition: BBa_B0034

BBa_I0500
type: Component
persistent identity: component2398599
display id: component2398599
version: 1
top level: BBa_K1486056
access: public
definition: pBad/araC

BBa_K1486000
type: Component
persistent identity: component2398605
display id: component2398605
version: 1
top level: BBa_K1486056
access: public
definition: BBa_K1486000

BBa_K1486004
type: Component
persistent identity: component2398606
display id: component2398606
version: 1
top level: BBa_K1486056
access: public
definition: BBa_K1486004

BBa_K1486044
type: Component
persistent identity: component2398607
display id: component2398607
version: 1
top level: BBa_K1486056
access: public
definition: BBa_K1486044

BBa_K1486004
type: SequenceAnnotation
persistent identity: annotation2398606
display id: annotation2398606
version: 1
top level: BBa_K1486056
location: https://synbiohub.org/public/igem/BBa_K1486056/annotation2398606/range2398606/1
component: BBa_K1486004

https://synbiohub.org/public/igem/BBa_K1486056/annotation2398606/range2398606/1
type: Range
persistent identity: range2398606
display id: range2398606
version: 1
direction: direction/reverse
top level: BBa_K1486056
start: 1947
end: 1976
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2398609
display id: annotation2398609
version: 1
top level: BBa_K1486056
location: https://synbiohub.org/public/igem/BBa_K1486056/annotation2398609/range2398609/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1486056/annotation2398609/range2398609/1
type: Range
persistent identity: range2398609
display id: range2398609
version: 1
direction: direction/reverse
top level: BBa_K1486056
start: 2395
end: 2406
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2398601
display id: annotation2398601
version: 1
top level: BBa_K1486056
location: https://synbiohub.org/public/igem/BBa_K1486056/annotation2398601/range2398601/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1486056/annotation2398601/range2398601/1
type: Range
persistent identity: range2398601
display id: range2398601
version: 1
direction: direction/reverse
top level: BBa_K1486056
start: 1219
end: 1230
orientation: inline

BBa_K1486044
type: SequenceAnnotation
persistent identity: annotation2398607
display id: annotation2398607
version: 1
top level: BBa_K1486056
location: https://synbiohub.org/public/igem/BBa_K1486056/annotation2398607/range2398607/1
component: BBa_K1486044

https://synbiohub.org/public/igem/BBa_K1486056/annotation2398607/range2398607/1
type: Range
persistent identity: range2398607
display id: range2398607
version: 1
direction: direction/reverse
top level: BBa_K1486056
start: 1985
end: 2386
orientation: inline

BBa_I0500
type: SequenceAnnotation
persistent identity: annotation2398599
display id: annotation2398599
version: 1
top level: BBa_K1486056
location: https://synbiohub.org/public/igem/BBa_K1486056/annotation2398599/range2398599/1
component: BBa_I0500

https://synbiohub.org/public/igem/BBa_K1486056/annotation2398599/range2398599/1
type: Range
persistent identity: range2398599
display id: range2398599
version: 1
direction: direction/reverse
top level: BBa_K1486056
start: 1
end: 1210
orientation: inline

BBa_K1486045
type: SequenceAnnotation
persistent identity: annotation2398615
display id: annotation2398615
version: 1
top level: BBa_K1486056
location: https://synbiohub.org/public/igem/BBa_K1486056/annotation2398615/range2398615/1
component: BBa_K1486045

https://synbiohub.org/public/igem/BBa_K1486056/annotation2398615/range2398615/1
type: Range
persistent identity: range2398615
display id: range2398615
version: 1
direction: direction/reverse
top level: BBa_K1486056
start: 3161
end: 3742
orientation: inline

BBa_K1486000
type: SequenceAnnotation
persistent identity: annotation2398605
display id: annotation2398605
version: 1
top level: BBa_K1486056
location: https://synbiohub.org/public/igem/BBa_K1486056/annotation2398605/range2398605/1
component: BBa_K1486000

https://synbiohub.org/public/igem/BBa_K1486056/annotation2398605/range2398605/1
type: Range
persistent identity: range2398605
display id: range2398605
version: 1
direction: direction/reverse
top level: BBa_K1486056
start: 1237
end: 1938
orientation: inline

BBa_K1486004
type: SequenceAnnotation
persistent identity: annotation2398614
display id: annotation2398614
version: 1
top level: BBa_K1486056
location: https://synbiohub.org/public/igem/BBa_K1486056/annotation2398614/range2398614/1
component: BBa_K1486004

https://synbiohub.org/public/igem/BBa_K1486056/annotation2398614/range2398614/1
type: Range
persistent identity: range2398614
display id: range2398614
version: 1
direction: direction/reverse
top level: BBa_K1486056
start: 3123
end: 3152
orientation: inline

BBa_K1486000
type: SequenceAnnotation
persistent identity: annotation2398613
display id: annotation2398613
version: 1
top level: BBa_K1486056
location: https://synbiohub.org/public/igem/BBa_K1486056/annotation2398613/range2398613/1
component: BBa_K1486000

https://synbiohub.org/public/igem/BBa_K1486056/annotation2398613/range2398613/1
type: Range
persistent identity: range2398613
display id: range2398613
version: 1
direction: direction/reverse
top level: BBa_K1486056
start: 2413
end: 3114
orientation: inline

BBa_I712004
type: ComponentDefinition
persistent identity: BBa I712004
display id: BBa_I712004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CMV promoter
creator: Andrej Ondracka
created: 2007-10-18T11:00:00Z
modified: 2015-08-31T04:07:45Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _130_
m user id: 0
owner id: 1896
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: a constitutive expression promoter or use in mammalian cells. Ribosome binding site is included.
mutable notes: Cloned into pSB1AC3 vector.
mutable provenance: pcDNA3 by Invitrogen
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I712004_sequence/1

BBa_K112407
type: ComponentDefinition
persistent identity: BBa K112407
display id: BBa_K112407
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter for ygeF psuedogene
creator: Christina Brown
created: 2008-10-20T11:00:00Z
modified: 2015-05-08T01:09:17Z
discontinued: false
dominant: false
group u list: _224_
m user id: 0
owner id: 3241
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. In a Bca1256 plasmid backbone. Our microarray results showed that this gene was upregulated four fold in response to low frequency ultrasound and two fold in response to audible sound. We cloned approximately 400 base pairs upstream from the start codon for the ygeF pseudogene.
mutable notes: none
mutable provenance: Cloned from E. coli MG1655 genome
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K112407_sequence/1

BBa_J54120
type: ComponentDefinition
persistent identity: BBa J54120
display id: BBa_J54120
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: EmrR_regulated promoter
creator: Yusaku Nakashima
created: 2006-10-20T11:00:00Z
modified: 2015-08-31T03:48:12Z
discontinued: false
dominant: false
group u list: _76_
m user id: 0
owner id: 762
owning group id: 76
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: EmrR_regulated promoter
mutable notes: AS EmrR regulated
mutable provenance: DNA synthesis
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: BamHI
sequence annotation: BetI_regulated promoter
sequence: https://synbiohub.org/public/igem/BBa_J54120_sequence/1

BamHI
type: SequenceAnnotation
persistent identity: annotation1903850
display id: annotation1903850
version: 1
top level: BBa_J54120
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_J54120/annotation1903850/range1903850/1

https://synbiohub.org/public/igem/BBa_J54120/annotation1903850/range1903850/1
type: Range
persistent identity: range1903850
display id: range1903850
version: 1
direction: direction/forward
top level: BBa_J54120
start: 1
end: 5
orientation: inline

BetI_regulated promoter
type: SequenceAnnotation
persistent identity: annotation1903851
display id: annotation1903851
version: 1
top level: BBa_J54120
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_J54120/annotation1903851/range1903851/1

https://synbiohub.org/public/igem/BBa_J54120/annotation1903851/range1903851/1
type: Range
persistent identity: range1903851
display id: range1903851
version: 1
direction: direction/forward
top level: BBa_J54120
start: 6
end: 43
orientation: inline

NAND2717
type: ComponentDefinition
persistent identity: BBa I732302
display id: BBa_I732302
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: NAND Candidate (U073O27D001O17)
creator: Ma Rui
created: 2007-10-02T11:00:00Z
modified: 2015-08-31T04:07:57Z
discontinued: false
dominant: false
experience: experience/Issues
group u list: _156_
m user id: 0
owner id: 1592
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: U97NUL D001O22
mutable notes: .
mutable provenance: .
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732302_sequence/1

BBa_J64808
type: ComponentDefinition
persistent identity: BBa J64808
display id: BBa_J64808
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RocE RBS
creator: David Stanley
created: 2007-03-19T12:00:00Z
modified: 2015-05-08T01:08:17Z
discontinued: false
dominant: false
experience: experience/None
group u list: _98_
m user id: 0
owner id: 1423
owning group id: 98
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: RBS preceding RocE
mutable notes: n
mutable provenance: n
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J64808_sequence/1

pJEJAM17
type: ComponentDefinition
persistent identity: BBa K678065
display id: BBa_K678065
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pJEJAM17
creator: DTU-Denmark-2
created: 2011-09-12T11:00:00Z
modified: 2015-05-08T01:13:05Z
discontinued: false
dominant: false
experience: experience/None
group u list: _882_
m user id: 0
owner id: 8341
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: To be announced
mutable notes: To be announced
mutable provenance: To be announced
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000155
role: partType/Plasmid
component: BBa_K678042
component: BBa_K678036
component: BBa_K678025
component: BBa_K678029
component: BBa_K678024
component: BBa_K678046
sequence annotation: BBa_K678036
sequence annotation: BBa_K678046
sequence annotation: BBa_K678042
sequence annotation: BBa_K678024
sequence annotation: BBa_K678029
sequence annotation: BBa_K678025
sequence: https://synbiohub.org/public/igem/BBa_K678065_sequence/1

BBa_K678042
type: Component
persistent identity: component2127584
display id: component2127584
version: 1
top level: pJEJAM17
access: public
definition: hygR

BBa_K678036
type: Component
persistent identity: component2127581
display id: component2127581
version: 1
top level: pJEJAM17
access: public
definition: TtrpC

BBa_K678025
type: Component
persistent identity: component2127575
display id: component2127575
version: 1
top level: pJEJAM17
access: public
definition: MTS

BBa_K678029
type: Component
persistent identity: component2127578
display id: component2127578
version: 1
top level: pJEJAM17
access: public
definition: GFP-TS

BBa_K678024
type: Component
persistent identity: component2127572
display id: component2127572
version: 1
top level: pJEJAM17
access: public
definition: PgpdA

BBa_K678046
type: Component
persistent identity: component2127587
display id: component2127587
version: 1
top level: pJEJAM17
access: public
definition: pFun

BBa_K678036
type: SequenceAnnotation
persistent identity: annotation2127581
display id: annotation2127581
version: 1
top level: pJEJAM17
location: https://synbiohub.org/public/igem/BBa_K678065/annotation2127581/range2127581/1
component: BBa_K678036

https://synbiohub.org/public/igem/BBa_K678065/annotation2127581/range2127581/1
type: Range
persistent identity: range2127581
display id: range2127581
version: 1
direction: direction/reverse
top level: pJEJAM17
start: 3254
end: 4012
orientation: inline

BBa_K678046
type: SequenceAnnotation
persistent identity: annotation2127587
display id: annotation2127587
version: 1
top level: pJEJAM17
location: https://synbiohub.org/public/igem/BBa_K678065/annotation2127587/range2127587/1
component: BBa_K678046

https://synbiohub.org/public/igem/BBa_K678065/annotation2127587/range2127587/1
type: Range
persistent identity: range2127587
display id: range2127587
version: 1
direction: direction/reverse
top level: pJEJAM17
start: 5392
end: 8269
orientation: inline

BBa_K678042
type: SequenceAnnotation
persistent identity: annotation2127584
display id: annotation2127584
version: 1
top level: pJEJAM17
location: https://synbiohub.org/public/igem/BBa_K678065/annotation2127584/range2127584/1
component: BBa_K678042

https://synbiohub.org/public/igem/BBa_K678065/annotation2127584/range2127584/1
type: Range
persistent identity: range2127584
display id: range2127584
version: 1
direction: direction/reverse
top level: pJEJAM17
start: 4021
end: 5383
orientation: inline

BBa_K678024
type: SequenceAnnotation
persistent identity: annotation2127572
display id: annotation2127572
version: 1
top level: pJEJAM17
location: https://synbiohub.org/public/igem/BBa_K678065/annotation2127572/range2127572/1
component: BBa_K678024

https://synbiohub.org/public/igem/BBa_K678065/annotation2127572/range2127572/1
type: Range
persistent identity: range2127572
display id: range2127572
version: 1
direction: direction/reverse
top level: pJEJAM17
start: 1
end: 2312
orientation: inline

BBa_K678029
type: SequenceAnnotation
persistent identity: annotation2127578
display id: annotation2127578
version: 1
top level: pJEJAM17
location: https://synbiohub.org/public/igem/BBa_K678065/annotation2127578/range2127578/1
component: BBa_K678029

https://synbiohub.org/public/igem/BBa_K678065/annotation2127578/range2127578/1
type: Range
persistent identity: range2127578
display id: range2127578
version: 1
direction: direction/reverse
top level: pJEJAM17
start: 2517
end: 3245
orientation: inline

BBa_K678025
type: SequenceAnnotation
persistent identity: annotation2127575
display id: annotation2127575
version: 1
top level: pJEJAM17
location: https://synbiohub.org/public/igem/BBa_K678065/annotation2127575/range2127575/1
component: BBa_K678025

https://synbiohub.org/public/igem/BBa_K678065/annotation2127575/range2127575/1
type: Range
persistent identity: range2127575
display id: range2127575
version: 1
direction: direction/reverse
top level: pJEJAM17
start: 2321
end: 2508
orientation: inline

BBa_K1520025
type: ComponentDefinition
persistent identity: BBa K1520025
display id: BBa_K1520025
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Ptet-rbs-rfp-Ter-Plac-rbs-tetR-Ter
creator: yue wu
created: 2014-09-21T11:00:00Z
modified: 2015-05-08T01:10:49Z
discontinued: false
dominant: false
group u list: _1902_
m user id: 0
owner id: 21318
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Ptet-rbs-rfp-Ter-Plac-rbs-tetR-Ter. It produces rfp when there is enough lacI or less lactose.
mutable notes: None
mutable provenance: Ptet, rfp, Plac, tetR, Ter
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1520014
component: BBa_K1520015
sequence annotation: BBa_K1520014
sequence annotation: BBa_K1520015
sequence: https://synbiohub.org/public/igem/BBa_K1520025_sequence/1

BBa_K1520014
type: Component
persistent identity: component2385272
display id: component2385272
version: 1
top level: BBa_K1520025
access: public
definition: BBa_K1520014

BBa_K1520015
type: Component
persistent identity: component2385284
display id: component2385284
version: 1
top level: BBa_K1520025
access: public
definition: https://synbiohub.org/public/igem/BBa_K1520015/1

BBa_K1520014
type: SequenceAnnotation
persistent identity: annotation2385272
display id: annotation2385272
version: 1
top level: BBa_K1520025
location: https://synbiohub.org/public/igem/BBa_K1520025/annotation2385272/range2385272/1
component: BBa_K1520014

https://synbiohub.org/public/igem/BBa_K1520025/annotation2385272/range2385272/1
type: Range
persistent identity: range2385272
display id: range2385272
version: 1
direction: direction/reverse
top level: BBa_K1520025
start: 1
end: 931
orientation: inline

BBa_K1520015
type: SequenceAnnotation
persistent identity: annotation2385284
display id: annotation2385284
version: 1
top level: BBa_K1520025
location: https://synbiohub.org/public/igem/BBa_K1520025/annotation2385284/range2385284/1
component: BBa_K1520015

https://synbiohub.org/public/igem/BBa_K1520025/annotation2385284/range2385284/1
type: Range
persistent identity: range2385284
display id: range2385284
version: 1
direction: direction/reverse
top level: BBa_K1520025
start: 940
end: 1987
orientation: inline

BBa_K338062
type: ComponentDefinition
persistent identity: BBa K338062
display id: BBa_K338062
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: GFP Reporter Device, Heat-Shock or IPTG Induced
creator: Lucas Hartsough
created: 2010-10-26T11:00:00Z
modified: 2015-05-08T01:12:07Z
discontinued: false
dominant: false
group u list: _454_
m user id: 0
owner id: 6013
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Construct assembled to allow the quantitative analysis of the heat shock/LacI promoter/repressor system, BBa_K338002 . Produces GFP in response to low-temperature heat shock, more-so in the presence of LacI. GFP production is retarded in LacIq cells (or in the presence of +5mM glucose) about 4-6x. See K338002 for more detailed data.
mutable notes: The type of GFP used in this construct happens to have a very long half life. If growing cultures for fluorescence measurements, grow at 30??C - any higher will activate the promoter, causing GFP to be produced.
mutable provenance: The Registry of Standard Biological Parts
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0015
component: BBa_B0034
component: BBa_K338002
component: BBa_E0040
sequence annotation: BBa_K338002
sequence annotation: BBa_B0034
sequence annotation: BBa_E0040
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_K338062_sequence/1

BBa_B0015
type: Component
persistent identity: component2105145
display id: component2105145
version: 1
top level: BBa_K338062
access: public
definition: BBa_B0015

BBa_B0034
type: Component
persistent identity: component2105136
display id: component2105136
version: 1
top level: BBa_K338062
access: public
definition: BBa_B0034

BBa_K338002
type: Component
persistent identity: component2105134
display id: component2105134
version: 1
top level: BBa_K338062
access: public
definition: HSP-LacI

BBa_E0040
type: Component
persistent identity: component2105138
display id: component2105138
version: 1
top level: BBa_K338062
access: public
definition: https://synbiohub.org/public/igem/BBa_E0040/1

BBa_K338002
type: SequenceAnnotation
persistent identity: annotation2105134
display id: annotation2105134
version: 1
top level: BBa_K338062
location: https://synbiohub.org/public/igem/BBa_K338062/annotation2105134/range2105134/1
component: BBa_K338002

https://synbiohub.org/public/igem/BBa_K338062/annotation2105134/range2105134/1
type: Range
persistent identity: range2105134
display id: range2105134
version: 1
direction: direction/reverse
top level: BBa_K338062
start: 1
end: 181
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2105136
display id: annotation2105136
version: 1
top level: BBa_K338062
location: https://synbiohub.org/public/igem/BBa_K338062/annotation2105136/range2105136/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K338062/annotation2105136/range2105136/1
type: Range
persistent identity: range2105136
display id: range2105136
version: 1
direction: direction/reverse
top level: BBa_K338062
start: 190
end: 201
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation2105138
display id: annotation2105138
version: 1
top level: BBa_K338062
location: https://synbiohub.org/public/igem/BBa_K338062/annotation2105138/range2105138/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_K338062/annotation2105138/range2105138/1
type: Range
persistent identity: range2105138
display id: range2105138
version: 1
direction: direction/reverse
top level: BBa_K338062
start: 208
end: 927
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2105145
display id: annotation2105145
version: 1
top level: BBa_K338062
location: https://synbiohub.org/public/igem/BBa_K338062/annotation2105145/range2105145/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K338062/annotation2105145/range2105145/1
type: Range
persistent identity: range2105145
display id: range2105145
version: 1
direction: direction/reverse
top level: BBa_K338062
start: 936
end: 1064
orientation: inline

BBa_K119002
type: ComponentDefinition
persistent identity: BBa K119002
display id: BBa_K119002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RcnR operator (represses RcnA)
creator: Libertad Pantoja
created: 2008-09-27T11:00:00Z
modified: 2015-05-08T01:09:38Z
discontinued: false
dominant: false
group u list: _235_
m user id: 0
owner id: 3054
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Operator for RcnR(YohL) the negative regulator of niquel extrusion pump(RcnA) in E.coli
mutable notes: We needed the RcnR operator without its promoter, so it was necessary to predict the promoter and discard it from the sequence.
mutable provenance: E.coli genome
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: RcnRop
sequence: https://synbiohub.org/public/igem/BBa_K119002_sequence/1

RcnRop
type: SequenceAnnotation
persistent identity: annotation1977507
display id: annotation1977507
version: 1
top level: BBa_K119002
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K119002/annotation1977507/range1977507/1

https://synbiohub.org/public/igem/BBa_K119002/annotation1977507/range1977507/1
type: Range
persistent identity: range1977507
display id: range1977507
version: 1
direction: direction/forward
top level: BBa_K119002
start: 1
end: 83
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TE from coliphageT7
creator: Reshma Shetty
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Transcription terminator for the E.coli RNA polymerase.
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 V01146 .
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: polya
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
direction: direction/forward
top level: BBa_B0012
start: 28
end: 41
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
direction: direction/forward
top level: BBa_B0012
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
direction: direction/forward
top level: BBa_B0012
start: 8
end: 27
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
direction: direction/forward
top level: BBa_B0012
start: 34
end: 34
orientation: inline

Oct-4 prom
type: ComponentDefinition
persistent identity: BBa I10498
display id: BBa_I10498
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Oct-4 promoter
creator: Amanda Morris
created: 2006-05-13T11:00:00Z
modified: 2015-08-31T04:07:30Z
discontinued: false
dominant: false
experience: experience/None
group u list: _89_
m user id: 0
owner id: 622
owning group id: 89
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: The part codes for the human Oct-4 promoter, which regulates expression of the Oct-4 gene. The Oct-4 gene is essential for pluripotency in cells and is downregulated once differentiation occurs. Sequence information is from GenBank (NM_002701) and shows the cis-regulatory mRNA that controls transcriptional activation of the human Oct-4 promoter.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I10498_sequence/1

BBa_K2084000
type: ComponentDefinition
persistent identity: BBa K2084000
display id: BBa_K2084000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: T3 Promoter
creator: Maya Lemmon-Kishi
created: 2016-10-11T11:00:00Z
modified: 2016-10-29T09:13:21Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2552_
m user id: 32066
owner id: 32131
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Promoter for the T3 RNA Polymerase.
mutable notes: NA
mutable provenance: Wild-type found in T3 bacteriophage. Synthesized by IDT.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K2084000_sequence/1

Kat
type: ComponentDefinition
persistent identity: BBa I14034
display id: BBa_I14034
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: P(Kat)
creator: Vikram Vijayan, Allen Hsu, Lawrence Fomundam
created: 2004-08-03T11:00:00Z
modified: 2015-08-31T04:07:38Z
discontinued: false
dominant: false
experience: experience/None
group u list: _4_
m user id: 0
owner id: 171
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Constitutive Promoter, Medium Transcription
mutable provenance: nnpp promoter 3 from pSB2K3
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: P(Kat)
sequence: https://synbiohub.org/public/igem/BBa_I14034_sequence/1

P(Kat)
type: SequenceAnnotation
persistent identity: annotation1023116
display id: annotation1023116
version: 1
top level: Kat
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I14034/annotation1023116/range1023116/1

https://synbiohub.org/public/igem/BBa_I14034/annotation1023116/range1023116/1
type: Range
persistent identity: range1023116
display id: range1023116
version: 1
direction: direction/forward
top level: Kat
start: 1
end: 45
orientation: inline

U073O11+D0
type: ComponentDefinition
persistent identity: BBa I732428
display id: BBa_I732428
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Family Member (U073O11+D038O22)
creator: Zhan Jian
created: 2007-10-13T11:00:00Z
modified: 2015-08-31T04:07:58Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732428_sequence/1

BBa_M13509
type: ComponentDefinition
persistent identity: BBa M13509
display id: BBa_M13509
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: M13Ko7 gene IX RBS
creator: Natalie Kuldell
created: 2006-12-14T12:00:00Z
modified: 2015-05-08T01:13:56Z
discontinued: false
dominant: false
experience: experience/None
group u list: _45_
m user id: 0
owner id: 314
owning group id: 1
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This RBS is part of the bacteriophage M13 genome, initiating translation of the gene IX message (BBa_M13009). The part aligns with base pairs 1190-1205 in M13K07 genome. It was identified as the RBS for gene IX message by Wezenbeek et al in Gene (1980) 11: 129-148
mutable notes: none
mutable provenance: New England Biolabs M13K07. Sequenced in 2002 by L. Panganaban
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_M13509_sequence/1

BBa_K678039
type: ComponentDefinition
persistent identity: BBa K678039
display id: BBa_K678039
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: T3-motni, terminator for Aspergillus niger
creator: DTU-Denmark-2
created: 2011-09-03T11:00:00Z
modified: 2015-05-08T01:13:04Z
discontinued: false
dominant: false
experience: experience/None
group u list: _882_
m user id: 0
owner id: 8341
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: T3-motni is a terminator for use in Aspergillus niger.
mutable notes: Compatible with the Plug'n'Play assembly standard
mutable provenance: Aspergillus niger genomic DNA
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: Linker 4
sequence annotation: T3-motni terminator
sequence: https://synbiohub.org/public/igem/BBa_K678039_sequence/1

Linker 4
type: SequenceAnnotation
persistent identity: annotation2127409
display id: annotation2127409
version: 1
top level: BBa_K678039
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K678039/annotation2127409/range2127409/1

https://synbiohub.org/public/igem/BBa_K678039/annotation2127409/range2127409/1
type: Range
persistent identity: range2127409
display id: range2127409
version: 1
direction: direction/forward
top level: BBa_K678039
start: 1
end: 8
orientation: inline

T3-motni terminator
type: SequenceAnnotation
persistent identity: annotation2127410
display id: annotation2127410
version: 1
top level: BBa_K678039
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K678039/annotation2127410/range2127410/1

https://synbiohub.org/public/igem/BBa_K678039/annotation2127410/range2127410/1
type: Range
persistent identity: range2127410
display id: range2127410
version: 1
direction: direction/forward
top level: BBa_K678039
start: 9
end: 889
orientation: inline

BBa_Z0253
type: ComponentDefinition
persistent identity: BBa Z0253
display id: BBa_Z0253
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: T7 weak binding promoter
creator: Sriram Kosuri
created: 2006-05-15T11:00:00Z
modified: 2015-05-08T01:14:55Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_10_
m user id: 0
owner id: 64
owning group id: 10
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: ag->TA from concensus
sequence annotation: ag->GA from concensus
sequence: https://synbiohub.org/public/igem/BBa_Z0253_sequence/1

ag->TA from concensus
type: SequenceAnnotation
persistent identity: annotation1862684
display id: annotation1862684
version: 1
top level: BBa_Z0253
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_Z0253/annotation1862684/range1862684/1

https://synbiohub.org/public/igem/BBa_Z0253/annotation1862684/range1862684/1
type: Range
persistent identity: range1862684
display id: range1862684
version: 1
direction: direction/forward
top level: BBa_Z0253
start: 27
end: 28
orientation: inline

ag->GA from concensus
type: SequenceAnnotation
persistent identity: annotation1862691
display id: annotation1862691
version: 1
top level: BBa_Z0253
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_Z0253/annotation1862691/range1862691/1

https://synbiohub.org/public/igem/BBa_Z0253/annotation1862691/range1862691/1
type: Range
persistent identity: range1862691
display id: range1862691
version: 1
direction: direction/forward
top level: BBa_Z0253
start: 31
end: 32
orientation: inline

pJEJAM13
type: ComponentDefinition
persistent identity: BBa K678061
display id: BBa_K678061
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pJEJAM13
creator: DTU-Denmark-2
created: 2011-09-12T11:00:00Z
modified: 2015-05-08T01:13:05Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _882_
m user id: 0
owner id: 8341
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: To be announced
mutable notes: To be announced
mutable provenance: To be announced
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000155
role: partType/Plasmid
component: BBa_K678036
component: BBa_K678033
component: BBa_K678046
component: BBa_K678024
component: BBa_K678040
sequence annotation: BBa_K678024
sequence annotation: BBa_K678046
sequence annotation: BBa_K678040
sequence annotation: BBa_K678033
sequence annotation: BBa_K678036
sequence: https://synbiohub.org/public/igem/BBa_K678061_sequence/1

BBa_K678036
type: Component
persistent identity: component2135919
display id: component2135919
version: 1
top level: pJEJAM13
access: public
definition: TtrpC

BBa_K678033
type: Component
persistent identity: component2135916
display id: component2135916
version: 1
top level: pJEJAM13
access: public
definition: GFP-PTS1

BBa_K678046
type: Component
persistent identity: component2135925
display id: component2135925
version: 1
top level: pJEJAM13
access: public
definition: pFun

BBa_K678024
type: Component
persistent identity: component2135911
display id: component2135911
version: 1
top level: pJEJAM13
access: public
definition: PgpdA

BBa_K678040
type: Component
persistent identity: component2135922
display id: component2135922
version: 1
top level: pJEJAM13
access: public
definition: pyrG casse

BBa_K678024
type: SequenceAnnotation
persistent identity: annotation2135911
display id: annotation2135911
version: 1
top level: pJEJAM13
location: https://synbiohub.org/public/igem/BBa_K678061/annotation2135911/range2135911/1
component: BBa_K678024

https://synbiohub.org/public/igem/BBa_K678061/annotation2135911/range2135911/1
type: Range
persistent identity: range2135911
display id: range2135911
version: 1
direction: direction/reverse
top level: pJEJAM13
start: 1
end: 2312
orientation: inline

BBa_K678046
type: SequenceAnnotation
persistent identity: annotation2135925
display id: annotation2135925
version: 1
top level: pJEJAM13
location: https://synbiohub.org/public/igem/BBa_K678061/annotation2135925/range2135925/1
component: BBa_K678046

https://synbiohub.org/public/igem/BBa_K678061/annotation2135925/range2135925/1
type: Range
persistent identity: range2135925
display id: range2135925
version: 1
direction: direction/reverse
top level: pJEJAM13
start: 5265
end: 8142
orientation: inline

BBa_K678040
type: SequenceAnnotation
persistent identity: annotation2135922
display id: annotation2135922
version: 1
top level: pJEJAM13
location: https://synbiohub.org/public/igem/BBa_K678061/annotation2135922/range2135922/1
component: BBa_K678040

https://synbiohub.org/public/igem/BBa_K678061/annotation2135922/range2135922/1
type: Range
persistent identity: range2135922
display id: range2135922
version: 1
direction: direction/reverse
top level: pJEJAM13
start: 3809
end: 5264
orientation: inline

BBa_K678033
type: SequenceAnnotation
persistent identity: annotation2135916
display id: annotation2135916
version: 1
top level: pJEJAM13
location: https://synbiohub.org/public/igem/BBa_K678061/annotation2135916/range2135916/1
component: BBa_K678033

https://synbiohub.org/public/igem/BBa_K678061/annotation2135916/range2135916/1
type: Range
persistent identity: range2135916
display id: range2135916
version: 1
direction: direction/reverse
top level: pJEJAM13
start: 2313
end: 3049
orientation: inline

BBa_K678036
type: SequenceAnnotation
persistent identity: annotation2135919
display id: annotation2135919
version: 1
top level: pJEJAM13
location: https://synbiohub.org/public/igem/BBa_K678061/annotation2135919/range2135919/1
component: BBa_K678036

https://synbiohub.org/public/igem/BBa_K678061/annotation2135919/range2135919/1
type: Range
persistent identity: range2135919
display id: range2135919
version: 1
direction: direction/reverse
top level: pJEJAM13
start: 3050
end: 3808
orientation: inline

BBa_K266006
type: ComponentDefinition
persistent identity: BBa K266006
display id: BBa_K266006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PAI+LasR -> LasI+GFP & AI+LuxR --| LasI+GFP
creator: UNAM FCIENCIAS-CINVESTAV IRA
created: 2009-10-12T11:00:00Z
modified: 2015-05-08T01:11:43Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _363_
m user id: 0
owner id: 1955
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: BBa_K091146 promoter (PAI+LasR inducible & AI+LuxR repressible) controls the policistronic expresion of LasI enzyme and GFP, double terminator
mutable notes: None.
mutable provenance: The biobricks BBa_K266005 and BBa_E0840 where used to build this biobrick.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Signalling
role: SO:0000804
component: BBa_K266005
component: BBa_E0840
sequence annotation: BBa_E0840
sequence annotation: BBa_K266005
sequence: https://synbiohub.org/public/igem/BBa_K266006_sequence/1

BBa_K266005
type: Component
persistent identity: component2267607
display id: component2267607
version: 1
top level: BBa_K266006
access: public
definition: BBa_K266005

BBa_E0840
type: Component
persistent identity: component2267618
display id: component2267618
version: 1
top level: BBa_K266006
access: public
definition: https://synbiohub.org/public/igem/BBa_E0840/1

BBa_E0840
type: SequenceAnnotation
persistent identity: annotation2267618
display id: annotation2267618
version: 1
top level: BBa_K266006
location: https://synbiohub.org/public/igem/BBa_K266006/annotation2267618/range2267618/1
component: BBa_E0840

https://synbiohub.org/public/igem/BBa_K266006/annotation2267618/range2267618/1
type: Range
persistent identity: range2267618
display id: range2267618
version: 1
direction: direction/reverse
top level: BBa_K266006
start: 828
end: 1705
orientation: inline

BBa_K266005
type: SequenceAnnotation
persistent identity: annotation2267607
display id: annotation2267607
version: 1
top level: BBa_K266006
location: https://synbiohub.org/public/igem/BBa_K266006/annotation2267607/range2267607/1
component: BBa_K266005

https://synbiohub.org/public/igem/BBa_K266006/annotation2267607/range2267607/1
type: Range
persistent identity: range2267607
display id: range2267607
version: 1
direction: direction/reverse
top level: BBa_K266006
start: 1
end: 819
orientation: inline

BBa_K1585117
type: ComponentDefinition
persistent identity: BBa K1585117
display id: BBa_K1585117
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Anderson Promoter with lacI binding site
creator: Tobias Schwanemann
created: 2015-08-31T11:00:00Z
modified: 2015-09-01T02:23:31Z
discontinued: false
dominant: false
experience: experience/Fails
group u list: _2002_
m user id: 24474
owner id: 24474
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Promoters of the Anderson Promoter Collection with lacI binding site derived from [http://parts.igem.org/Part:BBa_R0010 R_0010] to make them [http://openwetware.org/wiki/IPTG IPTG] iducible.
mutable notes: We choosed lacI-binding-site of part BBa_R0010 because we assumed older parts would be more reliable. The part is shipped in backbone [http://parts.igem.org/Part:BBa_J61002 J61002] because the functionality is directly visible by adding IPTG. RFP ([http://parts.igem.org/Part:BBa_E1010 E1010]) should get expressed then.
mutable provenance: Assambly of [http://parts.igem.org/Part:BBa_J23116 J23116] and lacI-binding-site of [http://parts.igem.org/Part:BBa_R0010 R_0010] The "lacI binding site" of Part [http://parts.igem.org/Part:BBa_R0010 R_0010] differs from lacO from Part [http://parts.igem.org/Part:BBa_J33207 BBa_J33207].
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
component: BBa_K1585999
component: BBa_J23117
sequence annotation: BBa_J23117
sequence annotation: BBa_K1585999
sequence: https://synbiohub.org/public/igem/BBa_K1585117_sequence/1

BBa_K1585999
type: Component
persistent identity: component2441713
display id: component2441713
version: 1
top level: BBa_K1585117
access: public
definition: https://synbiohub.org/public/igem/BBa_K1585999/1

BBa_J23117
type: Component
persistent identity: component2441711
display id: component2441711
version: 1
top level: BBa_K1585117
access: public
definition: BBa_J23117

BBa_J23117
type: SequenceAnnotation
persistent identity: annotation2441711
display id: annotation2441711
version: 1
top level: BBa_K1585117
location: https://synbiohub.org/public/igem/BBa_K1585117/annotation2441711/range2441711/1
component: BBa_J23117

https://synbiohub.org/public/igem/BBa_K1585117/annotation2441711/range2441711/1
type: Range
persistent identity: range2441711
display id: range2441711
version: 1
direction: direction/reverse
top level: BBa_K1585117
start: 1
end: 35
orientation: inline

BBa_K1585999
type: SequenceAnnotation
persistent identity: annotation2441713
display id: annotation2441713
version: 1
top level: BBa_K1585117
location: https://synbiohub.org/public/igem/BBa_K1585117/annotation2441713/range2441713/1
component: BBa_K1585999

https://synbiohub.org/public/igem/BBa_K1585117/annotation2441713/range2441713/1
type: Range
persistent identity: range2441713
display id: range2441713
version: 1
direction: direction/reverse
top level: BBa_K1585117
start: 44
end: 78
orientation: inline

BBa_R2113
type: ComponentDefinition
persistent identity: BBa R2113
display id: BBa_R2113
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter with operator site for C2003
creator: Reshma Shetty
created: 2006-10-20T11:00:00Z
modified: 2015-05-08T01:14:16Z
discontinued: false
dominant: false
group u list: _41_
m user id: 0
owner id: 126
owning group id: 70
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This promoter is designed to bind to C2003.
mutable notes: The placement of the operator site was designed to be near optimal for steric interference between C2003 and RNA polymerase.
mutable provenance: This promoter was designed rationally.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: transcription start site
sequence annotation: C2003 operator site
sequence annotation: -35 site
sequence annotation: -10 site
sequence: https://synbiohub.org/public/igem/BBa_R2113_sequence/1

transcription start site
type: SequenceAnnotation
persistent identity: annotation1904657
display id: annotation1904657
version: 1
top level: BBa_R2113
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_R2113/annotation1904657/range1904657/1

https://synbiohub.org/public/igem/BBa_R2113/annotation1904657/range1904657/1
type: Range
persistent identity: range1904657
display id: range1904657
version: 1
direction: direction/forward
top level: BBa_R2113
start: 53
end: 53
orientation: inline

C2003 operator site
type: SequenceAnnotation
persistent identity: annotation1904658
display id: annotation1904658
version: 1
top level: BBa_R2113
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_R2113/annotation1904658/range1904658/1

https://synbiohub.org/public/igem/BBa_R2113/annotation1904658/range1904658/1
type: Range
persistent identity: range1904658
display id: range1904658
version: 1
direction: direction/forward
top level: BBa_R2113
start: 21
end: 32
orientation: inline

-35 site
type: SequenceAnnotation
persistent identity: annotation1904655
display id: annotation1904655
version: 1
top level: BBa_R2113
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R2113/annotation1904655/range1904655/1

https://synbiohub.org/public/igem/BBa_R2113/annotation1904655/range1904655/1
type: Range
persistent identity: range1904655
display id: range1904655
version: 1
direction: direction/forward
top level: BBa_R2113
start: 17
end: 22
orientation: inline

-10 site
type: SequenceAnnotation
persistent identity: annotation1904656
display id: annotation1904656
version: 1
top level: BBa_R2113
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R2113/annotation1904656/range1904656/1

https://synbiohub.org/public/igem/BBa_R2113/annotation1904656/range1904656/1
type: Range
persistent identity: range1904656
display id: range1904656
version: 1
direction: direction/forward
top level: BBa_R2113
start: 40
end: 45
orientation: inline

BBa_K1486016
type: ComponentDefinition
persistent identity: BBa K1486016
display id: BBa_K1486016
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: fLuc[1]
creator: EPFL iGem team 2014
created: 2014-09-14T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is the first part of the split Firefly luciferase. To be used with fLuc[2].
mutable notes: to edit
mutable provenance: to edit
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence: https://synbiohub.org/public/igem/BBa_K1486016_sequence/1

BBa_J61112
type: ComponentDefinition
persistent identity: BBa J61112
display id: BBa_J61112
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Ribosome Binding Site Family Member
creator: John Anderson
created: 2007-04-23T11:00:00Z
modified: 2015-08-31T02:03:00Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: false
mutable description: {{JCA_Arkin_RBSFamily}}
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J61112_sequence/1

U097NUL+D0
type: ComponentDefinition
persistent identity: BBa I732400
display id: BBa_I732400
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Family Member (U097NUL+D062NUL)
creator: Zhan Jian
created: 2007-10-03T11:00:00Z
modified: 2015-08-31T04:07:58Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1572
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: P_template1 serves as the truss for PCR cloning. By respectively applying 7 different primers upstream and downstream, that is, 49 pairs of primers in total, we have got 49 different promoters. Then we made them digsted and loaded into a vector that has already possessed a lacZ-alpha fragment. In this way, we can read from the color of the clones on the plate whether the inserted promoter works or not. By this means, We can try seven different positions for Operator A and seven for Operator B, thus constructing 7*7 kinds of promoters which have actually constituted a specific promoter family. After transformed into the four test plasmids (pCR00, pCR01, pCR10, pCR11) together with a downstream double-reporter system, we will get to know the solo-repression and co-repression effects of the two repressors on the specific promoter.
mutable notes: None.
mutable provenance: None.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732400_sequence/1

BBa_I715003
type: ComponentDefinition
persistent identity: BBa I715003
display id: BBa_I715003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: hybrid pLac with UV5 mutation
creator: Michael Waters
created: 2007-07-02T11:00:00Z
modified: 2015-08-31T04:07:49Z
discontinued: false
dominant: false
experience: experience/Issues
group u list: _120_
m user id: 0
owner id: 1761
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This promoter sequence is a derivative of part BBa_R0011 which is a -35 and -10 recognition site with two lac operator 1's inserted in the spacer region of the RNAP recognition region and before the -35 RANP recognition region. This part utilizes the UV5 double mutation at the -10 RNAP recognition site to recruit RNAP without the help of a CAP binding region.
mutable notes: The length of the fragment makes it cumbersome to gel purify
mutable provenance: E.Coli genome
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: lac 01
sequence annotation: UV5 -10 RNAP recognition region double mutation
sequence annotation: -10 RNAP binding
sequence annotation: -35 RNAP binding
sequence: https://synbiohub.org/public/igem/BBa_I715003_sequence/1

lac 01
type: SequenceAnnotation
persistent identity: annotation1936229
display id: annotation1936229
version: 1
top level: BBa_I715003
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_I715003/annotation1936229/range1936229/1

https://synbiohub.org/public/igem/BBa_I715003/annotation1936229/range1936229/1
type: Range
persistent identity: range1936229
display id: range1936229
version: 1
direction: direction/forward
top level: BBa_I715003
start: 3
end: 19
orientation: inline

UV5 -10 RNAP recognition region double mutation
type: SequenceAnnotation
persistent identity: annotation1936232
display id: annotation1936232
version: 1
top level: BBa_I715003
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_I715003/annotation1936232/range1936232/1

https://synbiohub.org/public/igem/BBa_I715003/annotation1936232/range1936232/1
type: Range
persistent identity: range1936232
display id: range1936232
version: 1
direction: direction/forward
top level: BBa_I715003
start: 43
end: 47
orientation: inline

-10 RNAP binding
type: SequenceAnnotation
persistent identity: annotation1936231
display id: annotation1936231
version: 1
top level: BBa_I715003
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I715003/annotation1936231/range1936231/1

https://synbiohub.org/public/igem/BBa_I715003/annotation1936231/range1936231/1
type: Range
persistent identity: range1936231
display id: range1936231
version: 1
direction: direction/forward
top level: BBa_I715003
start: 43
end: 47
orientation: inline

lac 01
type: SequenceAnnotation
persistent identity: annotation1936230
display id: annotation1936230
version: 1
top level: BBa_I715003
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_I715003/annotation1936230/range1936230/1

https://synbiohub.org/public/igem/BBa_I715003/annotation1936230/range1936230/1
type: Range
persistent identity: range1936230
display id: range1936230
version: 1
direction: direction/forward
top level: BBa_I715003
start: 26
end: 42
orientation: inline

-35 RNAP binding
type: SequenceAnnotation
persistent identity: annotation1936228
display id: annotation1936228
version: 1
top level: BBa_I715003
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I715003/annotation1936228/range1936228/1

https://synbiohub.org/public/igem/BBa_I715003/annotation1936228/range1936228/1
type: Range
persistent identity: range1936228
display id: range1936228
version: 1
direction: direction/forward
top level: BBa_I715003
start: 20
end: 25
orientation: inline

BBa_K1486012
type: ComponentDefinition
persistent identity: BBa K1486012
display id: BBa_K1486012
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CpxR-IFP[1] under pBAD
creator: iGem EPFL 2014
created: 2014-08-31T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part has a fused protein downstream of an arabinose-inducible promoter: CpxR-IFP[1]. Biobrick BBa_K1486013 has CpxR-IFP[2] with an arabinose promoter. This construct was made in both orientations: BBa_K1486014 is IFP[1]-CpxR (with an arabinose promoter).
mutable notes: Both orientations were made, as well as for IFP[2] so all dimerizations could be tested (see main page for biobrick numbers). Flexible linkers were used to fuse both proteins, and an RBS was put before each one of them.
mutable provenance: To Edit
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K1486004
component: BBa_K1486000
component: BBa_K1486006
component: BBa_B0034
component: BBa_I0500
sequence annotation: BBa_B0034
sequence annotation: BBa_K1486004
sequence annotation: BBa_K1486000
sequence annotation: BBa_K1486006
sequence annotation: BBa_I0500
sequence: https://synbiohub.org/public/igem/BBa_K1486012_sequence/1

BBa_K1486004
type: Component
persistent identity: component2382292
display id: component2382292
version: 1
top level: BBa_K1486012
access: public
definition: BBa_K1486004

BBa_K1486000
type: Component
persistent identity: component2382291
display id: component2382291
version: 1
top level: BBa_K1486012
access: public
definition: BBa_K1486000

BBa_K1486006
type: Component
persistent identity: component2382293
display id: component2382293
version: 1
top level: BBa_K1486012
access: public
definition: BBa_K1486006

BBa_B0034
type: Component
persistent identity: component2382287
display id: component2382287
version: 1
top level: BBa_K1486012
access: public
definition: BBa_B0034

BBa_I0500
type: Component
persistent identity: component2382285
display id: component2382285
version: 1
top level: BBa_K1486012
access: public
definition: pBad/araC

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2382287
display id: annotation2382287
version: 1
top level: BBa_K1486012
location: https://synbiohub.org/public/igem/BBa_K1486012/annotation2382287/range2382287/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1486012/annotation2382287/range2382287/1
type: Range
persistent identity: range2382287
display id: range2382287
version: 1
direction: direction/reverse
top level: BBa_K1486012
start: 1211
end: 1222
orientation: inline

BBa_K1486004
type: SequenceAnnotation
persistent identity: annotation2382292
display id: annotation2382292
version: 1
top level: BBa_K1486012
location: https://synbiohub.org/public/igem/BBa_K1486012/annotation2382292/range2382292/1
component: BBa_K1486004

https://synbiohub.org/public/igem/BBa_K1486012/annotation2382292/range2382292/1
type: Range
persistent identity: range2382292
display id: range2382292
version: 1
direction: direction/reverse
top level: BBa_K1486012
start: 1925
end: 1954
orientation: inline

BBa_K1486000
type: SequenceAnnotation
persistent identity: annotation2382291
display id: annotation2382291
version: 1
top level: BBa_K1486012
location: https://synbiohub.org/public/igem/BBa_K1486012/annotation2382291/range2382291/1
component: BBa_K1486000

https://synbiohub.org/public/igem/BBa_K1486012/annotation2382291/range2382291/1
type: Range
persistent identity: range2382291
display id: range2382291
version: 1
direction: direction/reverse
top level: BBa_K1486012
start: 1223
end: 1924
orientation: inline

BBa_K1486006
type: SequenceAnnotation
persistent identity: annotation2382293
display id: annotation2382293
version: 1
top level: BBa_K1486012
location: https://synbiohub.org/public/igem/BBa_K1486012/annotation2382293/range2382293/1
component: BBa_K1486006

https://synbiohub.org/public/igem/BBa_K1486012/annotation2382293/range2382293/1
type: Range
persistent identity: range2382293
display id: range2382293
version: 1
direction: direction/reverse
top level: BBa_K1486012
start: 1955
end: 2536
orientation: inline

BBa_I0500
type: SequenceAnnotation
persistent identity: annotation2382285
display id: annotation2382285
version: 1
top level: BBa_K1486012
location: https://synbiohub.org/public/igem/BBa_K1486012/annotation2382285/range2382285/1
component: BBa_I0500

https://synbiohub.org/public/igem/BBa_K1486012/annotation2382285/range2382285/1
type: Range
persistent identity: range2382285
display id: range2382285
version: 1
direction: direction/reverse
top level: BBa_K1486012
start: 1
end: 1210
orientation: inline

BBa_K086028
type: ComponentDefinition
persistent identity: BBa K086028
display id: BBa_K086028
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: modified Lutz-Bujard LacO promoter,with alternative sigma factor σ32
creator: Gairik Sachdeva
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:08:37Z
discontinued: false
dominant: false
group u list: _197_
m user id: 0
owner id: 2578
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: modified Lutz-Bujard LacO promoter,with alternative sigma factor σ32
mutable notes: cleaned
mutable provenance: Lac O and sigma 32 binding sites' promoter sequences
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: P32_3
sequence: https://synbiohub.org/public/igem/BBa_K086028_sequence/1

P32_3
type: SequenceAnnotation
persistent identity: annotation1995762
display id: annotation1995762
version: 1
top level: BBa_K086028
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K086028/annotation1995762/range1995762/1

https://synbiohub.org/public/igem/BBa_K086028/annotation1995762/range1995762/1
type: Range
persistent identity: range1995762
display id: range1995762
version: 1
direction: direction/forward
top level: BBa_K086028
start: 1
end: 55
orientation: inline

U085O11+D0
type: ComponentDefinition
persistent identity: BBa I732419
display id: BBa_I732419
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Family Member (U085O11+D002O22)
creator: Zhan Jian
created: 2007-10-13T11:00:00Z
modified: 2015-08-31T04:07:58Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732419_sequence/1

BBa_I766555
type: ComponentDefinition
persistent identity: BBa I766555
display id: BBa_I766555
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pCyc (Medium) Promoter
creator: Nili Sommovilla
created: 2007-08-15T11:00:00Z
modified: 2015-08-31T04:08:10Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _155_
m user id: 0
owner id: 1931
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Mid-expression level constitutive promoter in yeast
mutable notes: none
mutable provenance: Endogenous promoter for Cytochrome c
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I766555_sequence/1

Pnot(O11)
type: ComponentDefinition
persistent identity: BBa I732201
display id: BBa_I732201
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: NOT Gate Promoter Family Member (D001O11)
creator: Zhan Jian
created: 2007-10-13T11:00:00Z
modified: 2015-08-31T04:07:57Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732201_sequence/1

BBa_R2112
type: ComponentDefinition
persistent identity: BBa R2112
display id: BBa_R2112
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter with operator site for C2003
creator: Reshma Shetty
created: 2006-10-20T11:00:00Z
modified: 2015-05-08T01:14:16Z
discontinued: false
dominant: false
group u list: _41_
m user id: 0
owner id: 126
owning group id: 70
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This promoter is designed to bind to C2003.
mutable notes: The placement of the operator site was designed to be near optimal for steric interference between C2003 and RNA polymerase.
mutable provenance: This promoter was rationally designed.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -35 site
sequence annotation: -10 site
sequence annotation: transcription start site
sequence annotation: C2003 operator site
sequence: https://synbiohub.org/public/igem/BBa_R2112_sequence/1

-35 site
type: SequenceAnnotation
persistent identity: annotation1903924
display id: annotation1903924
version: 1
top level: BBa_R2112
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R2112/annotation1903924/range1903924/1

https://synbiohub.org/public/igem/BBa_R2112/annotation1903924/range1903924/1
type: Range
persistent identity: range1903924
display id: range1903924
version: 1
direction: direction/forward
top level: BBa_R2112
start: 17
end: 22
orientation: inline

-10 site
type: SequenceAnnotation
persistent identity: annotation1903925
display id: annotation1903925
version: 1
top level: BBa_R2112
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R2112/annotation1903925/range1903925/1

https://synbiohub.org/public/igem/BBa_R2112/annotation1903925/range1903925/1
type: Range
persistent identity: range1903925
display id: range1903925
version: 1
direction: direction/forward
top level: BBa_R2112
start: 40
end: 45
orientation: inline

transcription start site
type: SequenceAnnotation
persistent identity: annotation1903926
display id: annotation1903926
version: 1
top level: BBa_R2112
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_R2112/annotation1903926/range1903926/1

https://synbiohub.org/public/igem/BBa_R2112/annotation1903926/range1903926/1
type: Range
persistent identity: range1903926
display id: range1903926
version: 1
direction: direction/forward
top level: BBa_R2112
start: 53
end: 53
orientation: inline

C2003 operator site
type: SequenceAnnotation
persistent identity: annotation1903927
display id: annotation1903927
version: 1
top level: BBa_R2112
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_R2112/annotation1903927/range1903927/1

https://synbiohub.org/public/igem/BBa_R2112/annotation1903927/range1903927/1
type: Range
persistent identity: range1903927
display id: range1903927
version: 1
direction: direction/forward
top level: BBa_R2112
start: 31
end: 42
orientation: inline

BBa_J15301
type: ComponentDefinition
persistent identity: BBa J15301
display id: BBa_J15301
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Pars promoter from Escherichia coli chromosomal ars operon.
creator: Chris French
created: 2007-07-12T11:00:00Z
modified: 2015-08-31T04:08:32Z
discontinued: false
dominant: false
group u list: _163_
m user id: 0
owner id: 837
owning group id: 163
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is the promoter region of the Escherichia coli chromosomal ars promoter. It is repressed by ArsR in the absence of arsenate or arsenite (Cai, J., and DuBow, M.S. 1996. Expression of the Escherichia coli chromosomal ars operon. Canadian Journal of Microbiology 42, 662-671; Diorio, C., Cai, J., Marmor, J., Shinder, R., and DuBow, M.S. 1995. An Escherichia coli chromosomal ars operon homolog is functional in arsenic detoxification and is conserved in Gram negative bacteria. Journal of Bacteriology 177, 2050-2056).
mutable notes: No modifications were made to the sequence. There appears to be an unexpected repeat of ACT immediately before the SpeI site. This does not affect the biobrick suffix but means that the suffix is 5 bases downstream of the annotated transcriptional start site rather than 2 as recommended. The reason for this is unclear.
mutable provenance: Escherichia coli K12. Sequence obtained from X80057 (gi:510824). Regulatory sequence annotation from Cai, J., and DuBow, M.S. 1996. Expression of the Escherichia coli chromosomal ars operon. Canadian Journal of Microbiology 42, 662-671; Diorio, C., Cai, J., Marmor, J., Shinder, R., and DuBow, M.S. 1995. An Escherichia coli chromosomal ars operon homolog is functional in arsenic detoxification and is conserved in Gram negative bacteria. Journal of Bacteriology 177, 2050-2056.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -35
sequence annotation: ArsR binding
sequence annotation: -10
sequence annotation: +1
sequence: https://synbiohub.org/public/igem/BBa_J15301_sequence/1

-35
type: SequenceAnnotation
persistent identity: annotation1938076
display id: annotation1938076
version: 1
top level: BBa_J15301
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_J15301/annotation1938076/range1938076/1

https://synbiohub.org/public/igem/BBa_J15301/annotation1938076/range1938076/1
type: Range
persistent identity: range1938076
display id: range1938076
version: 1
direction: direction/forward
top level: BBa_J15301
start: 88
end: 93
orientation: inline

ArsR binding
type: SequenceAnnotation
persistent identity: annotation1938075
display id: annotation1938075
version: 1
top level: BBa_J15301
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_J15301/annotation1938075/range1938075/1

https://synbiohub.org/public/igem/BBa_J15301/annotation1938075/range1938075/1
type: Range
persistent identity: range1938075
display id: range1938075
version: 1
direction: direction/forward
top level: BBa_J15301
start: 81
end: 85
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1938077
display id: annotation1938077
version: 1
top level: BBa_J15301
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_J15301/annotation1938077/range1938077/1

https://synbiohub.org/public/igem/BBa_J15301/annotation1938077/range1938077/1
type: Range
persistent identity: range1938077
display id: range1938077
version: 1
direction: direction/forward
top level: BBa_J15301
start: 111
end: 116
orientation: inline

ArsR binding
type: SequenceAnnotation
persistent identity: annotation1938074
display id: annotation1938074
version: 1
top level: BBa_J15301
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_J15301/annotation1938074/range1938074/1

https://synbiohub.org/public/igem/BBa_J15301/annotation1938074/range1938074/1
type: Range
persistent identity: range1938074
display id: range1938074
version: 1
direction: direction/forward
top level: BBa_J15301
start: 67
end: 71
orientation: inline

+1
type: SequenceAnnotation
persistent identity: annotation1938078
display id: annotation1938078
version: 1
top level: BBa_J15301
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J15301/annotation1938078/range1938078/1

https://synbiohub.org/public/igem/BBa_J15301/annotation1938078/range1938078/1
type: Range
persistent identity: range1938078
display id: range1938078
version: 1
direction: direction/forward
top level: BBa_J15301
start: 123
end: 123
orientation: inline

BBa_K1480004
type: ComponentDefinition
persistent identity: BBa K1480004
display id: BBa_K1480004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TetR generator (Promoter J23106 )
creator: Jesus Gilberto Rodriguez Ceja
created: 2014-10-13T11:00:00Z
modified: 2015-05-08T01:10:40Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1860_
m user id: 0
owner id: 12878
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is a TetR protein generator regulated by the constitutive promoter J23106. The protein has a LVA tag for degradation.
mutable notes: Based on parts of registry.
mutable provenance: It was assembled using assembly 10.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_J23106
component: BBa_B0012
component: BBa_C0040
component: BBa_B0010
component: BBa_B0034
sequence annotation: BBa_C0040
sequence annotation: BBa_B0010
sequence annotation: BBa_B0034
sequence annotation: BBa_J23106
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_K1480004_sequence/1

BBa_J23106
type: Component
persistent identity: component2419541
display id: component2419541
version: 1
top level: BBa_K1480004
access: public
definition: BBa_J23106

BBa_B0012
type: Component
persistent identity: component2419550
display id: component2419550
version: 1
top level: BBa_K1480004
access: public
definition: BBa_B0012

BBa_C0040
type: Component
persistent identity: component2419547
display id: component2419547
version: 1
top level: BBa_K1480004
access: public
definition: https://synbiohub.org/public/igem/BBa_C0040/1

BBa_B0010
type: Component
persistent identity: component2419548
display id: component2419548
version: 1
top level: BBa_K1480004
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0034
type: Component
persistent identity: component2419543
display id: component2419543
version: 1
top level: BBa_K1480004
access: public
definition: BBa_B0034

BBa_C0040
type: SequenceAnnotation
persistent identity: annotation2419547
display id: annotation2419547
version: 1
top level: BBa_K1480004
location: https://synbiohub.org/public/igem/BBa_K1480004/annotation2419547/range2419547/1
component: BBa_C0040

https://synbiohub.org/public/igem/BBa_K1480004/annotation2419547/range2419547/1
type: Range
persistent identity: range2419547
display id: range2419547
version: 1
direction: direction/reverse
top level: BBa_K1480004
start: 62
end: 746
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2419548
display id: annotation2419548
version: 1
top level: BBa_K1480004
location: https://synbiohub.org/public/igem/BBa_K1480004/annotation2419548/range2419548/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K1480004/annotation2419548/range2419548/1
type: Range
persistent identity: range2419548
display id: range2419548
version: 1
direction: direction/forward
top level: BBa_K1480004
start: 755
end: 834
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2419543
display id: annotation2419543
version: 1
top level: BBa_K1480004
location: https://synbiohub.org/public/igem/BBa_K1480004/annotation2419543/range2419543/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1480004/annotation2419543/range2419543/1
type: Range
persistent identity: range2419543
display id: range2419543
version: 1
direction: direction/reverse
top level: BBa_K1480004
start: 44
end: 55
orientation: inline

BBa_J23106
type: SequenceAnnotation
persistent identity: annotation2419541
display id: annotation2419541
version: 1
top level: BBa_K1480004
location: https://synbiohub.org/public/igem/BBa_K1480004/annotation2419541/range2419541/1
component: BBa_J23106

https://synbiohub.org/public/igem/BBa_K1480004/annotation2419541/range2419541/1
type: Range
persistent identity: range2419541
display id: range2419541
version: 1
direction: direction/reverse
top level: BBa_K1480004
start: 1
end: 35
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2419550
display id: annotation2419550
version: 1
top level: BBa_K1480004
location: https://synbiohub.org/public/igem/BBa_K1480004/annotation2419550/range2419550/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K1480004/annotation2419550/range2419550/1
type: Range
persistent identity: range2419550
display id: range2419550
version: 1
direction: direction/forward
top level: BBa_K1480004
start: 843
end: 883
orientation: inline

BBa_M13505
type: ComponentDefinition
persistent identity: BBa M13505
display id: BBa_M13505
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: M13KO7 gene V RBS
creator: Natalie Kuldell
created: 2006-12-14T12:00:00Z
modified: 2015-05-08T01:13:56Z
discontinued: false
dominant: false
experience: experience/None
group u list: _45_
m user id: 0
owner id: 314
owning group id: 1
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This RBS is part of the bacteriophage M13 genome, initiating translation of the gene V message (BBa_M13005). The part aligns with base pairs 827-842 in M13K07 genome. It was identified as the RBS for the gene V message by Wezenbeek et al in Gene (1980) 11: 129-148
mutable notes: none
mutable provenance: New England Biolabs M13K07. Sequenced in 2002 by L. Panganaban
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_M13505_sequence/1

BBa_K1072005
type: ComponentDefinition
persistent identity: BBa K1072005
display id: BBa_K1072005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PT7+RBS+α-ALS+T7TM
creator: Xuenian chen, Zewei yan and Yichong wang
created: 2013-09-06T11:00:00Z
modified: 2015-05-08T01:09:01Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1381_
m user id: 0
owner id: 16002
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: α-ALS gene express acetolactate synthetase that contribute to produce diacetyl. The synthetize pathway of diacetyl, in E.coli, beginning with the central pathway metabolite pyruvate as the precursor molecule, the first step in the diacetyl biosynthetic pathway involves the condensation of two molecules of pyruvate to yield one molecule of acetolactate, and is catalyzed by acetolactate synthase (ALS).The stability of acetolactate is known to be affected by the presence of oxygen, which causes its spontaneous decarboxylation to produce diacetyl.
mutable notes: -
mutable provenance: originate from Lactococcus lactis
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: T7 Terminator
sequence annotation: alpha-ALS Coding Region
sequence annotation: T7 Promotor
sequence: https://synbiohub.org/public/igem/BBa_K1072005_sequence/1

T7 Terminator
type: SequenceAnnotation
persistent identity: annotation2352907
display id: annotation2352907
version: 1
top level: BBa_K1072005
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1072005/annotation2352907/range2352907/1

https://synbiohub.org/public/igem/BBa_K1072005/annotation2352907/range2352907/1
type: Range
persistent identity: range2352907
display id: range2352907
version: 1
direction: direction/forward
top level: BBa_K1072005
start: 1791
end: 1919
orientation: inline

alpha-ALS Coding Region
type: SequenceAnnotation
persistent identity: annotation2352912
display id: annotation2352912
version: 1
top level: BBa_K1072005
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1072005/annotation2352912/range2352912/1

https://synbiohub.org/public/igem/BBa_K1072005/annotation2352912/range2352912/1
type: Range
persistent identity: range2352912
display id: range2352912
version: 1
direction: direction/forward
top level: BBa_K1072005
start: 92
end: 1756
orientation: inline

T7 Promotor
type: SequenceAnnotation
persistent identity: annotation2352906
display id: annotation2352906
version: 1
top level: BBa_K1072005
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1072005/annotation2352906/range2352906/1

https://synbiohub.org/public/igem/BBa_K1072005/annotation2352906/range2352906/1
type: Range
persistent identity: range2352906
display id: range2352906
version: 1
direction: direction/forward
top level: BBa_K1072005
start: 1
end: 19
orientation: inline

Pnot(O46)
type: ComponentDefinition
persistent identity: BBa I732277
display id: BBa_I732277
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Family Member with Hybrid Operator (D001O46)
creator: Ma Rui
created: 2007-10-13T11:00:00Z
modified: 2015-08-31T04:07:57Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732277_sequence/1

BBa_K112708
type: ComponentDefinition
persistent identity: BBa K112708
display id: BBa_K112708
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PfhuA
creator: Bing Xia
created: 2008-10-20T11:00:00Z
modified: 2015-05-08T01:09:18Z
discontinued: false
dominant: false
group u list: _224_
m user id: 0
owner id: 2998
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: The fhuA promoter. ---- This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at: [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
mutable provenance: Construction of <PfhuA> basic part K112708 PCR of dv019/dv020 on MG1655 (225bp, EcoRI/BamHI/DpnI) Sub into pBca1256 (EcoRI/BamHI, 2472+225, L) Product is pBca1256-K112708 ----------------------------------------------- dv019 Forward biobricking of <PfhuA> cgataGAATTCatgAGATCTgctaagcgtgaaataccggatgg dv020 Reverse biobricking of <PfhuA> cgttaGGATCCactctgatgtaaagtgaatgataacg
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K112708_sequence/1

CP1LacPI1
type: ComponentDefinition
persistent identity: BBa K418000
display id: BBa_K418000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: IPTG inducible Lac promoter cassette
creator: Northwestern University iGEM 2010
created: 2010-10-11T11:00:00Z
modified: 2015-05-08T01:12:28Z
discontinued: false
dominant: true
experience: experience/None
group u list: _532_
m user id: 0
owner id: 6657
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: constituitive promoter + LacPI
mutable notes: blah
mutable provenance: From assembly of kit parts.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_Q01121
component: BBa_J23100
sequence annotation: BBa_J23100
sequence annotation: BBa_Q01121
sequence: https://synbiohub.org/public/igem/BBa_K418000_sequence/1

BBa_Q01121
type: Component
persistent identity: component2263742
display id: component2263742
version: 1
top level: CP1LacPI1
access: public
definition: https://synbiohub.org/public/igem/BBa_Q01121/1

BBa_J23100
type: Component
persistent identity: component2263723
display id: component2263723
version: 1
top level: CP1LacPI1
access: public
definition: BBa_J23100

BBa_J23100
type: SequenceAnnotation
persistent identity: annotation2263723
display id: annotation2263723
version: 1
top level: CP1LacPI1
location: https://synbiohub.org/public/igem/BBa_K418000/annotation2263723/range2263723/1
component: BBa_J23100

https://synbiohub.org/public/igem/BBa_K418000/annotation2263723/range2263723/1
type: Range
persistent identity: range2263723
display id: range2263723
version: 1
direction: direction/reverse
top level: CP1LacPI1
start: 1
end: 35
orientation: inline

BBa_Q01121
type: SequenceAnnotation
persistent identity: annotation2263742
display id: annotation2263742
version: 1
top level: CP1LacPI1
location: https://synbiohub.org/public/igem/BBa_K418000/annotation2263742/range2263742/1
component: BBa_Q01121

https://synbiohub.org/public/igem/BBa_K418000/annotation2263742/range2263742/1
type: Range
persistent identity: range2263742
display id: range2263742
version: 1
direction: direction/reverse
top level: CP1LacPI1
start: 44
end: 1416
orientation: inline

BBa_K427004
type: ComponentDefinition
persistent identity: BBa K427004
display id: BBa_K427004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Pmom promoter of Mu bacteriophage
creator: Jan Marte Contreras Ortiz
created: 2010-10-21T11:00:00Z
modified: 2015-05-08T01:12:29Z
discontinued: false
dominant: false
group u list: _544_
m user id: 0
owner id: 6602
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Pmom is one of the four late promoters of the Mu bacteriophage. It can be activated by the C protein, which is produced by the middle promoter Pm. Pmom works because of a special configuration of the DNA. It can be used to create a sensitivity tuner alongside the C protein of the same phage. This construction can be used to increase the POPS output of a promoter.
mutable notes: The design of this part had no special considerations
mutable provenance: Synthetized from the reported sequence of Pmom promoter from Mu bacteriophage
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: Pmom
sequence: https://synbiohub.org/public/igem/BBa_K427004_sequence/1

Pmom
type: SequenceAnnotation
persistent identity: annotation2092368
display id: annotation2092368
version: 1
top level: BBa_K427004
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K427004/annotation2092368/range2092368/1

https://synbiohub.org/public/igem/BBa_K427004/annotation2092368/range2092368/1
type: Range
persistent identity: range2092368
display id: range2092368
version: 1
direction: direction/forward
top level: BBa_K427004
start: 1
end: 79
orientation: inline

BBa_K2012000
type: ComponentDefinition
persistent identity: BBa K2012000
display id: BBa_K2012000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: c-di-GMP tandem riboswitche bc3-5
creator: Pan Chu
created: 2016-08-04T11:00:00Z
modified: 2016-10-09T01:16:18Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2479_
m user id: 25212
owner id: 25212
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Three complete c-di-GMP riboswitches (Bc3, Bc4 and Bc5 RNA) with similar structures, which are arranged in tandem to constitute a triple-tandem (Bc3-5 RNA) riboswitch in the 5′-UTR of the cspABCDE mRNA in Bacillus thuringiensis subsp. chinensis CT-43.
mutable notes: We designed the sequence of bc3-5 by add perfix and suffix which are belong to BioBrick RFC 10.
mutable provenance: From Bacillus thuringiensis subsp. chinensis CT-43 In the CT-43 genome19,20 (GenBank accession: NC_017208.1), five type I c-di-GMP riboswitches named Bc1, Bc2, Bc3, Bc4 and Bc5 RNA (Supplementary Tables S1???S5) were annotated in the Rfam database (http://rfam.sanger.ac.uk/search). Zhou, H., et al. (2016). "Characterization of a natural triple-tandem c-di-GMP riboswitch and application of the riboswitch-based dual-fluorescence reporter." Sci Rep 6: 20871.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Inverter
sequence: https://synbiohub.org/public/igem/BBa_K2012000_sequence/1

BBa_I765007
type: ComponentDefinition
persistent identity: BBa I765007
display id: BBa_I765007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Fe and UV promoters
creator: Claudia Guevara and Alex Quintero
created: 2007-10-25T11:00:00Z
modified: 2015-08-31T04:08:09Z
discontinued: false
dominant: false
experience: experience/None
group u list: _118_
m user id: 0
owner id: 2267
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Regulated by the presence of Iron and UV light irradiation
mutable notes: None
mutable provenance: Genomic sequence
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
component: BBa_I765000
component: BBa_I765001
sequence annotation: BBa_I765000
sequence annotation: BBa_I765001
sequence: https://synbiohub.org/public/igem/BBa_I765007_sequence/1

BBa_I765000
type: Component
persistent identity: component1958274
display id: component1958274
version: 1
top level: BBa_I765007
access: public
definition: https://synbiohub.org/public/igem/BBa_I765000/1

BBa_I765001
type: Component
persistent identity: component1958276
display id: component1958276
version: 1
top level: BBa_I765007
access: public
definition: pUV

BBa_I765000
type: SequenceAnnotation
persistent identity: annotation1958274
display id: annotation1958274
version: 1
top level: BBa_I765007
location: https://synbiohub.org/public/igem/BBa_I765007/annotation1958274/range1958274/1
component: BBa_I765000

https://synbiohub.org/public/igem/BBa_I765007/annotation1958274/range1958274/1
type: Range
persistent identity: range1958274
display id: range1958274
version: 1
direction: direction/reverse
top level: BBa_I765007
start: 1
end: 1044
orientation: inline

BBa_I765001
type: SequenceAnnotation
persistent identity: annotation1958276
display id: annotation1958276
version: 1
top level: BBa_I765007
location: https://synbiohub.org/public/igem/BBa_I765007/annotation1958276/range1958276/1
component: BBa_I765001

https://synbiohub.org/public/igem/BBa_I765007/annotation1958276/range1958276/1
type: Range
persistent identity: range1958276
display id: range1958276
version: 1
direction: direction/reverse
top level: BBa_I765007
start: 1053
end: 1128
orientation: inline

BBa_I746104
type: ComponentDefinition
persistent identity: BBa I746104
display id: BBa_I746104
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: P2 promoter in agr operon from S. aureus
creator: Zhizhen Zhao
created: 2007-08-30T11:00:00Z
modified: 2015-08-31T04:08:04Z
discontinued: false
dominant: false
experience: experience/None
group u list: _116_
m user id: 0
owner id: 2121
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: The agr P2 operon is an autocatalytic sensory transduction system in Staphylococcus aureus. P2 promoter regulates the synthesis of AgrB (a transmembrane protein) and AgrD (precursor of AIP autoinducing peptide). AIP binds to AgrC, a membrane binding receptor and it phosphorylates AgrA. The phosphorylated AgrA increases the expression level from P2 promoter.
mutable notes: Codon usage has been optimised for E. coli (which also seems to give good results for B. subtilis
mutable provenance: The section of the sequence constituting the P2 promoter is subject to rather more debate than the sequences of the coding regions. [http://jb.asm.org/cgi/content/full/186/22/7549 Koenig, Robbin L., Ray, Jessica L., Maleki, Soheila J., Smeltzer, Mark S., Hurlburt, Barry K. Staphylococcus aureus AgrA Binding to the RNAIII-agr Regulatory Region J. Bacteriol. 2004 186: 7549-7555] shows the areas of the sequence to which phosphorylated AgrA binds while L Rao, R K Karls, and M J Betley: In vitro transcription of pathogenesis-related genes by purified RNA polymerase from Staphylococcus aureus. Bacteriol. 1995 May; 177(10): 2609???2614. shows the transcription start sites; we took the overall extent of the promoter region from that labelled in Morfeldt, E., K. Tegmark, and S. Arvidson. 1996. Transcriptional control of the agr-dependent virulence gene regulator, RNAIII, in Staphylococcus aureus. Mol. Microbiol. 21:1227???1237. (not available online).
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I746104_sequence/1

BBa_K780000
type: ComponentDefinition
persistent identity: BBa K780000
display id: BBa_K780000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Terminator for Bacillus subtilis
creator: Nikodem Latocha
created: 2012-09-25T11:00:00Z
modified: 2015-05-08T01:13:18Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1034_
m user id: 0
owner id: 10113
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Our terminator is based on strong, Rho-independent terminator of gyrase gene, which is type II topoisomerase (EC 5.99.1.3). DNA gyrase uses the energy of ATP hydrolysis to introduce negative supercoils or relaxes positive supercoils. Negative DNA supercoiling is essential in vivo to compact the genome, relieve torsional strain during replication, and promote local melting for vital processes such as transcript initation by RNA polymerase. Gyrase gene is an housekeeping gene and because of that it must have efficient terminator.
mutable notes: -
mutable provenance: Terminator of gyrase (EC 5.99.1.3) gene.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence: https://synbiohub.org/public/igem/BBa_K780000_sequence/1

BBa_K086027
type: ComponentDefinition
persistent identity: BBa K086027
display id: BBa_K086027
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: modified Lutz-Bujard LacO promoter,with alternative sigma factor σ32
creator: Gairik Sachdeva
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:08:37Z
discontinued: false
dominant: false
group u list: _197_
m user id: 0
owner id: 2578
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: modified Lutz-Bujard LacO promoter,with alternative sigma factor σ32
mutable notes: cleaned
mutable provenance: Lac O and sigma 32 binding sites conserved sequences
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: P32_2
sequence: https://synbiohub.org/public/igem/BBa_K086027_sequence/1

P32_2
type: SequenceAnnotation
persistent identity: annotation1995761
display id: annotation1995761
version: 1
top level: BBa_K086027
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K086027/annotation1995761/range1995761/1

https://synbiohub.org/public/igem/BBa_K086027/annotation1995761/range1995761/1
type: Range
persistent identity: range1995761
display id: range1995761
version: 1
direction: direction/forward
top level: BBa_K086027
start: 1
end: 55
orientation: inline

eCFP
type: ComponentDefinition
persistent identity: BBa K678015
display id: BBa_K678015
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: eCFP-module
creator: DTU-Denmark-2
created: 2011-09-12T11:00:00Z
modified: 2015-05-08T01:13:04Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _882_
m user id: 0
owner id: 10556
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part was designed for the Plug'n'Play with DNA assembly standard (RFC 80), therefore this part contains tails at the 5' and 3' ends as shown on the figure below.
mutable notes: Ni
mutable provenance: The Danish Cancer Society
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: Linker 2
sequence annotation: eCFP
sequence annotation: Kozak
sequence: https://synbiohub.org/public/igem/BBa_K678015_sequence/1

Linker 2
type: SequenceAnnotation
persistent identity: annotation2127708
display id: annotation2127708
version: 1
top level: eCFP
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K678015/annotation2127708/range2127708/1

https://synbiohub.org/public/igem/BBa_K678015/annotation2127708/range2127708/1
type: Range
persistent identity: range2127708
display id: range2127708
version: 1
direction: direction/forward
top level: eCFP
start: 1
end: 8
orientation: inline

eCFP
type: SequenceAnnotation
persistent identity: annotation2127709
display id: annotation2127709
version: 1
top level: eCFP
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K678015/annotation2127709/range2127709/1

https://synbiohub.org/public/igem/BBa_K678015/annotation2127709/range2127709/1
type: Range
persistent identity: range2127709
display id: range2127709
version: 1
direction: direction/forward
top level: eCFP
start: 16
end: 813
orientation: inline

Kozak
type: SequenceAnnotation
persistent identity: annotation2127786
display id: annotation2127786
version: 1
top level: eCFP
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K678015/annotation2127786/range2127786/1

https://synbiohub.org/public/igem/BBa_K678015/annotation2127786/range2127786/1
type: Range
persistent identity: range2127786
display id: range2127786
version: 1
direction: direction/forward
top level: eCFP
start: 9
end: 19
orientation: inline

BBa_I723014
type: ComponentDefinition
persistent identity: BBa I723014
display id: BBa_I723014
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Estimated RBS for DntA
creator: Scott Ramsay
created: 2007-10-24T11:00:00Z
modified: 2015-08-31T04:07:53Z
discontinued: false
dominant: false
experience: experience/None
group u list: _126_
m user id: 0
owner id: 2140
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is the naturally-occurring RBS found in the regulatory region of the DntA gene.
mutable notes: '''''This does not physically exist as a basic part due to time constraints; it was cloned as part of composite part which incorporates all of the components necessary for expression of a reporter gene under the control of DntR'''''. For this reason, the ends of the supplied sequence may not accurately represent the boundaries of the functional unit.
mutable provenance: Cloned from a historical plasmid based on pQF52 carrying genomic sequences from ''Burkholderia cepacia''.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: misc
sequence: https://synbiohub.org/public/igem/BBa_I723014_sequence/1

misc
type: SequenceAnnotation
persistent identity: annotation1954961
display id: annotation1954961
version: 1
top level: BBa_I723014
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I723014/annotation1954961/range1954961/1

https://synbiohub.org/public/igem/BBa_I723014/annotation1954961/range1954961/1
type: Range
persistent identity: range1954961
display id: range1954961
version: 1
direction: direction/forward
top level: BBa_I723014
start: 1
end: 25
orientation: inline

BBa_J07007
type: ComponentDefinition
persistent identity: BBa J07007
display id: BBa_J07007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: ctx promoter
creator: igem_mit_2005
created: 2005-07-05T11:00:00Z
modified: 2015-08-31T04:08:19Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_12_21_1_
m user id: 0
owner id: 363
owning group id: 21
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: V.cholerae ctx gene promoter region. Transcription takes place In the presence of a ToxR dimer. We don't have the -10 and -35 sites annotated (need to do this).
mutable provenance: GENBANK M21250
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: ctx promoter
sequence annotation: transcription start
sequence annotation: -10 region
sequence: https://synbiohub.org/public/igem/BBa_J07007_sequence/1

ctx promoter
type: SequenceAnnotation
persistent identity: annotation1571612
display id: annotation1571612
version: 1
top level: BBa_J07007
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_J07007/annotation1571612/range1571612/1

https://synbiohub.org/public/igem/BBa_J07007/annotation1571612/range1571612/1
type: Range
persistent identity: range1571612
display id: range1571612
version: 1
direction: direction/forward
top level: BBa_J07007
start: 1
end: 145
orientation: inline

transcription start
type: SequenceAnnotation
persistent identity: annotation1577387
display id: annotation1577387
version: 1
top level: BBa_J07007
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J07007/annotation1577387/range1577387/1

https://synbiohub.org/public/igem/BBa_J07007/annotation1577387/range1577387/1
type: Range
persistent identity: range1577387
display id: range1577387
version: 1
direction: direction/forward
top level: BBa_J07007
start: 145
end: 145
orientation: inline

-10 region
type: SequenceAnnotation
persistent identity: annotation1579234
display id: annotation1579234
version: 1
top level: BBa_J07007
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J07007/annotation1579234/range1579234/1

https://synbiohub.org/public/igem/BBa_J07007/annotation1579234/range1579234/1
type: Range
persistent identity: range1579234
display id: range1579234
version: 1
direction: direction/forward
top level: BBa_J07007
start: 133
end: 138
orientation: inline

BBa_B0030
type: ComponentDefinition
persistent identity: BBa B0030
display id: BBa_B0030
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS.1 (strong) -- modified from R. Weiss
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _44_46_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>.
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("orig" in figure 4-14 of Ron Weiss thesis). No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info Brian Chow
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: BBa_B0030
sequence annotation: RBS-1\Strong
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_B0030_sequence/1

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation7025
display id: annotation7025
version: 1
top level: BBa_B0030
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_B0030/annotation7025/range7025/1

https://synbiohub.org/public/igem/BBa_B0030/annotation7025/range7025/1
type: Range
persistent identity: range7025
display id: range7025
version: 1
direction: direction/forward
top level: BBa_B0030
start: 1
end: 15
orientation: inline

RBS-1\Strong
type: SequenceAnnotation
persistent identity: annotation1701
display id: annotation1701
version: 1
top level: BBa_B0030
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_B0030/annotation1701/range1701/1

https://synbiohub.org/public/igem/BBa_B0030/annotation1701/range1701/1
type: Range
persistent identity: range1701
display id: range1701
version: 1
direction: direction/forward
top level: BBa_B0030
start: 1
end: 15
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation1702
display id: annotation1702
version: 1
top level: BBa_B0030
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_B0030/annotation1702/range1702/1

https://synbiohub.org/public/igem/BBa_B0030/annotation1702/range1702/1
type: Range
persistent identity: range1702
display id: range1702
version: 1
direction: direction/forward
top level: BBa_B0030
start: 8
end: 12
orientation: inline

BBa_K136000
type: ComponentDefinition
persistent identity: BBa K136000
display id: BBa_K136000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter of fliL gene from E. coli flagella
creator: Cyprien Maisonnier
created: 2008-08-11T11:00:00Z
modified: 2015-05-08T01:10:02Z
discontinued: true
dominant: false
experience: experience/None
group u list: _211_
m user id: 0
owner id: 3012
owning group id: 9
sample status: Discontinued
status: status/Deleted
bookmark: false
mutable description: ''fliL'' is a class 2 gene that is part of the construction of the flagella ( ''Ordering Genes in a Flagella Pathway by Analysis of Expression Kinetics from Living Bacteria'' - S. Kalir, J. McClure, K. Pabbaraju, C. Southward, M. Ronen, S. Leibler, M. G. Surette, U. Alon, ). Its promoter is consitutively repressed. It is activated by flhD4C2 and by fliA with different forces ( ''Using a Quantitative Blueprint to Reprogram the Dynamics of the Flagella Gene Network'' - Shiraz Kalir and Uri Alon ). A deeper characterization is in project
mutable notes: The primers used to amplify this sequence are : Forward : TCGAATTCGCGGCCGCTTCTAGAGCAAGGGCGTGTAACAGGCAAC Reverse : TCCTGCAGCGGCCGCTACTAGTAGTCATGTGTTGCGGTCTTCCTGTG
mutable provenance: This part comes from the genome of ''E.coli'' K12 strain MG1655.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: Initiation start for transcription 2
sequence annotation: FlhDC binding site 1
sequence annotation: FlhDC binding site 2
sequence annotation: Initiation start for transcription 1
sequence: https://synbiohub.org/public/igem/BBa_K136000_sequence/1

Initiation start for transcription 2
type: SequenceAnnotation
persistent identity: annotation1971567
display id: annotation1971567
version: 1
top level: BBa_K136000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K136000/annotation1971567/range1971567/1

https://synbiohub.org/public/igem/BBa_K136000/annotation1971567/range1971567/1
type: Range
persistent identity: range1971567
display id: range1971567
version: 1
direction: direction/forward
top level: BBa_K136000
start: 127
end: 127
orientation: inline

FlhDC binding site 1
type: SequenceAnnotation
persistent identity: annotation1971564
display id: annotation1971564
version: 1
top level: BBa_K136000
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K136000/annotation1971564/range1971564/1

https://synbiohub.org/public/igem/BBa_K136000/annotation1971564/range1971564/1
type: Range
persistent identity: range1971564
display id: range1971564
version: 1
direction: direction/forward
top level: BBa_K136000
start: 44
end: 59
orientation: inline

FlhDC binding site 2
type: SequenceAnnotation
persistent identity: annotation1971565
display id: annotation1971565
version: 1
top level: BBa_K136000
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K136000/annotation1971565/range1971565/1

https://synbiohub.org/public/igem/BBa_K136000/annotation1971565/range1971565/1
type: Range
persistent identity: range1971565
display id: range1971565
version: 1
direction: direction/forward
top level: BBa_K136000
start: 70
end: 85
orientation: inline

Initiation start for transcription 1
type: SequenceAnnotation
persistent identity: annotation1971566
display id: annotation1971566
version: 1
top level: BBa_K136000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K136000/annotation1971566/range1971566/1

https://synbiohub.org/public/igem/BBa_K136000/annotation1971566/range1971566/1
type: Range
persistent identity: range1971566
display id: range1971566
version: 1
direction: direction/forward
top level: BBa_K136000
start: 116
end: 116
orientation: inline

Pnot(O12)
type: ComponentDefinition
persistent identity: BBa I732270
display id: BBa_I732270
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Family Member with Hybrid Operator (D001O12)
creator: Zhan Jian
created: 2007-10-13T11:00:00Z
modified: 2015-08-31T04:07:57Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732270_sequence/1

BBa_K1520515
type: ComponentDefinition
persistent identity: BBa K1520515
display id: BBa_K1520515
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: MC7-rbs-luxI-Ter-Pcons2-rbs-luxR-Ter-Prlux-rbs-OMPA-golB-rbs-luxI-Ter.
creator: yue wu
created: 2014-09-22T11:00:00Z
modified: 2015-05-08T01:10:49Z
discontinued: false
dominant: false
group u list: _1902_
m user id: 0
owner id: 21318
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: MC7-rbs-luxI-Ter-Pcons2-rbs-luxR-Ter-Prlux-rbs-OMPA-golB-rbs-luxI-Ter. It is a test of our positive feedback circuit for microcystin.
mutable notes: None
mutable provenance: MC7, luxI, Pcons2, luxR, Prlux, OMPA-golB, Ter
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1520029
component: BBa_R0062
component: BBa_K1520504
component: BBa_K1520001
component: BBa_K1520507
component: BBa_K1520003
sequence annotation: BBa_K1520029
sequence annotation: BBa_K1520504
sequence annotation: BBa_R0062
sequence annotation: BBa_K1520507
sequence annotation: BBa_K1520003
sequence annotation: BBa_K1520001
sequence: https://synbiohub.org/public/igem/BBa_K1520515_sequence/1

BBa_K1520029
type: Component
persistent identity: component2385669
display id: component2385669
version: 1
top level: BBa_K1520515
access: public
definition: https://synbiohub.org/public/igem/BBa_K1520029/1

BBa_R0062
type: Component
persistent identity: component2385677
display id: component2385677
version: 1
top level: BBa_K1520515
access: public
definition: lux pR

BBa_K1520504
type: Component
persistent identity: component2385690
display id: component2385690
version: 1
top level: BBa_K1520515
access: public
definition: https://synbiohub.org/public/igem/BBa_K1520504/1

BBa_K1520001
type: Component
persistent identity: component2385665
display id: component2385665
version: 1
top level: BBa_K1520515
access: public
definition: https://synbiohub.org/public/igem/BBa_K1520001/1

BBa_K1520029
type: Component
persistent identity: component2385691
display id: component2385691
version: 1
top level: BBa_K1520515
access: public
definition: https://synbiohub.org/public/igem/BBa_K1520029/1

BBa_K1520507
type: Component
persistent identity: component2385687
display id: component2385687
version: 1
top level: BBa_K1520515
access: public
definition: https://synbiohub.org/public/igem/BBa_K1520507/1

BBa_K1520003
type: Component
persistent identity: component2385675
display id: component2385675
version: 1
top level: BBa_K1520515
access: public
definition: https://synbiohub.org/public/igem/BBa_K1520003/1

BBa_K1520504
type: Component
persistent identity: component2385668
display id: component2385668
version: 1
top level: BBa_K1520515
access: public
definition: https://synbiohub.org/public/igem/BBa_K1520504/1

BBa_K1520029
type: SequenceAnnotation
persistent identity: annotation2385669
display id: annotation2385669
version: 1
top level: BBa_K1520515
location: https://synbiohub.org/public/igem/BBa_K1520515/annotation2385669/range2385669/1
component: BBa_K1520029

https://synbiohub.org/public/igem/BBa_K1520515/annotation2385669/range2385669/1
type: Range
persistent identity: range2385669
display id: range2385669
version: 1
direction: direction/reverse
top level: BBa_K1520515
start: 811
end: 939
orientation: inline

BBa_K1520504
type: SequenceAnnotation
persistent identity: annotation2385668
display id: annotation2385668
version: 1
top level: BBa_K1520515
location: https://synbiohub.org/public/igem/BBa_K1520515/annotation2385668/range2385668/1
component: BBa_K1520504

https://synbiohub.org/public/igem/BBa_K1520515/annotation2385668/range2385668/1
type: Range
persistent identity: range2385668
display id: range2385668
version: 1
direction: direction/reverse
top level: BBa_K1520515
start: 142
end: 802
orientation: inline

BBa_K1520029
type: SequenceAnnotation
persistent identity: annotation2385691
display id: annotation2385691
version: 1
top level: BBa_K1520515
location: https://synbiohub.org/public/igem/BBa_K1520515/annotation2385691/range2385691/1
component: BBa_K1520029

https://synbiohub.org/public/igem/BBa_K1520515/annotation2385691/range2385691/1
type: Range
persistent identity: range2385691
display id: range2385691
version: 1
direction: direction/reverse
top level: BBa_K1520515
start: 3483
end: 3611
orientation: inline

BBa_R0062
type: SequenceAnnotation
persistent identity: annotation2385677
display id: annotation2385677
version: 1
top level: BBa_K1520515
location: https://synbiohub.org/public/igem/BBa_K1520515/annotation2385677/range2385677/1
component: BBa_R0062

https://synbiohub.org/public/igem/BBa_K1520515/annotation2385677/range2385677/1
type: Range
persistent identity: range2385677
display id: range2385677
version: 1
direction: direction/forward
top level: BBa_K1520515
start: 1935
end: 1989
orientation: inline

BBa_K1520504
type: SequenceAnnotation
persistent identity: annotation2385690
display id: annotation2385690
version: 1
top level: BBa_K1520515
location: https://synbiohub.org/public/igem/BBa_K1520515/annotation2385690/range2385690/1
component: BBa_K1520504

https://synbiohub.org/public/igem/BBa_K1520515/annotation2385690/range2385690/1
type: Range
persistent identity: range2385690
display id: range2385690
version: 1
direction: direction/reverse
top level: BBa_K1520515
start: 2814
end: 3474
orientation: inline

BBa_K1520507
type: SequenceAnnotation
persistent identity: annotation2385687
display id: annotation2385687
version: 1
top level: BBa_K1520515
location: https://synbiohub.org/public/igem/BBa_K1520515/annotation2385687/range2385687/1
component: BBa_K1520507

https://synbiohub.org/public/igem/BBa_K1520515/annotation2385687/range2385687/1
type: Range
persistent identity: range2385687
display id: range2385687
version: 1
direction: direction/reverse
top level: BBa_K1520515
start: 1996
end: 2805
orientation: inline

BBa_K1520003
type: SequenceAnnotation
persistent identity: annotation2385675
display id: annotation2385675
version: 1
top level: BBa_K1520515
location: https://synbiohub.org/public/igem/BBa_K1520515/annotation2385675/range2385675/1
component: BBa_K1520003

https://synbiohub.org/public/igem/BBa_K1520515/annotation2385675/range2385675/1
type: Range
persistent identity: range2385675
display id: range2385675
version: 1
direction: direction/reverse
top level: BBa_K1520515
start: 948
end: 1926
orientation: inline

BBa_K1520001
type: SequenceAnnotation
persistent identity: annotation2385665
display id: annotation2385665
version: 1
top level: BBa_K1520515
location: https://synbiohub.org/public/igem/BBa_K1520515/annotation2385665/range2385665/1
component: BBa_K1520001

https://synbiohub.org/public/igem/BBa_K1520515/annotation2385665/range2385665/1
type: Range
persistent identity: range2385665
display id: range2385665
version: 1
direction: direction/reverse
top level: BBa_K1520515
start: 1
end: 133
orientation: inline

CP2LacPI1
type: ComponentDefinition
persistent identity: BBa K418003
display id: BBa_K418003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: IPTG inducible Lac promoter cassette
creator: Northwestern University iGEM 2010
created: 2010-10-15T11:00:00Z
modified: 2015-05-08T01:12:28Z
discontinued: false
dominant: false
experience: experience/None
group u list: _532_
m user id: 0
owner id: 6938
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_Q01121
component: BBa_J23104
sequence annotation: BBa_Q01121
sequence annotation: BBa_J23104
sequence: https://synbiohub.org/public/igem/BBa_K418003_sequence/1

BBa_Q01121
type: Component
persistent identity: component2263782
display id: component2263782
version: 1
top level: CP2LacPI1
access: public
definition: https://synbiohub.org/public/igem/BBa_Q01121/1

BBa_J23104
type: Component
persistent identity: component2263763
display id: component2263763
version: 1
top level: CP2LacPI1
access: public
definition: BBa_J23104

BBa_Q01121
type: SequenceAnnotation
persistent identity: annotation2263782
display id: annotation2263782
version: 1
top level: CP2LacPI1
location: https://synbiohub.org/public/igem/BBa_K418003/annotation2263782/range2263782/1
component: BBa_Q01121

https://synbiohub.org/public/igem/BBa_K418003/annotation2263782/range2263782/1
type: Range
persistent identity: range2263782
display id: range2263782
version: 1
direction: direction/reverse
top level: CP2LacPI1
start: 44
end: 1416
orientation: inline

BBa_J23104
type: SequenceAnnotation
persistent identity: annotation2263763
display id: annotation2263763
version: 1
top level: CP2LacPI1
location: https://synbiohub.org/public/igem/BBa_K418003/annotation2263763/range2263763/1
component: BBa_J23104

https://synbiohub.org/public/igem/BBa_K418003/annotation2263763/range2263763/1
type: Range
persistent identity: range2263763
display id: range2263763
version: 1
direction: direction/reverse
top level: CP2LacPI1
start: 1
end: 35
orientation: inline

BBa_K094140
type: ComponentDefinition
persistent identity: BBa K094140
display id: BBa_K094140
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pLacIq
creator: Liu Chenli
created: 2008-10-12T11:00:00Z
modified: 2015-05-08T01:08:40Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _255_
m user id: 0
owner id: 2589
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: mutant promoter of lacI gene, which enables high level of lacI protein expression
mutable notes: no specific considerations
mutable provenance: point mutation made from E. Coli genomic lacI gene promoter
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: pLacIq
sequence: https://synbiohub.org/public/igem/BBa_K094140_sequence/1

pLacIq
type: SequenceAnnotation
persistent identity: annotation1981123
display id: annotation1981123
version: 1
top level: BBa_K094140
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K094140/annotation1981123/range1981123/1

https://synbiohub.org/public/igem/BBa_K094140/annotation1981123/range1981123/1
type: Range
persistent identity: range1981123
display id: range1981123
version: 1
direction: direction/forward
top level: BBa_K094140
start: 1
end: 80
orientation: inline

BBa_K1520014
type: ComponentDefinition
persistent identity: BBa K1520014
display id: BBa_K1520014
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Ptet-rbs-rfp-Ter
creator: yue wu
created: 2014-09-21T11:00:00Z
modified: 2015-05-08T01:10:49Z
discontinued: false
dominant: false
group u list: _1902_
m user id: 0
owner id: 21318
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Ptet-rbs-rfp-Ter. It produces rfp when there is less tetR.
mutable notes: None
mutable provenance: Ptet, rfp, Ter
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_R0040
component: BBa_K1520029
component: BBa_K1520500
sequence annotation: BBa_K1520029
sequence annotation: BBa_K1520500
sequence annotation: BBa_R0040
sequence: https://synbiohub.org/public/igem/BBa_K1520014_sequence/1

BBa_R0040
type: Component
persistent identity: component2384992
display id: component2384992
version: 1
top level: BBa_K1520014
access: public
definition: p(tetR)

BBa_K1520029
type: Component
persistent identity: component2384998
display id: component2384998
version: 1
top level: BBa_K1520014
access: public
definition: https://synbiohub.org/public/igem/BBa_K1520029/1

BBa_K1520500
type: Component
persistent identity: component2384997
display id: component2384997
version: 1
top level: BBa_K1520014
access: public
definition: https://synbiohub.org/public/igem/BBa_K1520500/1

BBa_K1520029
type: SequenceAnnotation
persistent identity: annotation2384998
display id: annotation2384998
version: 1
top level: BBa_K1520014
location: https://synbiohub.org/public/igem/BBa_K1520014/annotation2384998/range2384998/1
component: BBa_K1520029

https://synbiohub.org/public/igem/BBa_K1520014/annotation2384998/range2384998/1
type: Range
persistent identity: range2384998
display id: range2384998
version: 1
direction: direction/reverse
top level: BBa_K1520014
start: 803
end: 931
orientation: inline

BBa_K1520500
type: SequenceAnnotation
persistent identity: annotation2384997
display id: annotation2384997
version: 1
top level: BBa_K1520014
location: https://synbiohub.org/public/igem/BBa_K1520014/annotation2384997/range2384997/1
component: BBa_K1520500

https://synbiohub.org/public/igem/BBa_K1520014/annotation2384997/range2384997/1
type: Range
persistent identity: range2384997
display id: range2384997
version: 1
direction: direction/reverse
top level: BBa_K1520014
start: 63
end: 794
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation2384992
display id: annotation2384992
version: 1
top level: BBa_K1520014
location: https://synbiohub.org/public/igem/BBa_K1520014/annotation2384992/range2384992/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_K1520014/annotation2384992/range2384992/1
type: Range
persistent identity: range2384992
display id: range2384992
version: 1
direction: direction/forward
top level: BBa_K1520014
start: 1
end: 54
orientation: inline

Lock3
type: ComponentDefinition
persistent identity: BBa J01080
display id: BBa_J01080
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Lock3
creator: Golden Bear
created: 2005-11-29T12:00:00Z
modified: 2015-08-31T04:08:12Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _13_
m user id: 0
owner id: 395
owning group id: 13
sample status: In stock
status: status/Available
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000834
role: partType/RNA
sequence: https://synbiohub.org/public/igem/BBa_J01080_sequence/1

BBa_J61142
type: ComponentDefinition
persistent identity: BBa J61142
display id: BBa_J61142
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: {rbs1} Library Member in BBb
creator: John Anderson
created: 2007-09-03T11:00:00Z
modified: 2015-08-31T01:56:25Z
discontinued: false
dominant: false
experience: experience/None
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: {{BBbRBS1Family}}
mutable notes: {{BerkiGEM2007-Biobrick2.0Intro}}
mutable provenance: Synthetic
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J61142_sequence/1

BBa_J22052
type: ComponentDefinition
persistent identity: BBa J22052
display id: BBa_J22052
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Pcya
creator: Sugat Dabholkar
created: 2006-09-03T11:00:00Z
modified: 2015-08-31T04:08:38Z
discontinued: false
dominant: false
group u list: _70_44_
m user id: 0
owner id: 439
owning group id: 70
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is a adenylate cyclase promoter that has been reported to oscillate in cell cycle dependant manner.
mutable notes: No design problems
mutable provenance: Ref: J. Biol. Chem., Vol. 260, Issue 5, 3063-3070, 03, 1985 Transcription of the Escherichia coli adenylate cyclase gene is negatively regulated by cAMP-cAMP receptor protein H Aiba [bases -53 to 12]
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J22052_sequence/1

BBa_B0075
type: ComponentDefinition
persistent identity: BBa B0075
display id: BBa_B0075
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Orthogonal RBS
creator: Felix Moser
created: 2008-01-17T12:00:00Z
modified: 2015-08-31T04:07:21Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_
m user id: 0
owner id: 2398
owning group id: 11
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Predicted by Lon Chubiz to be RBS sequence with strongest affinity for T7 ribosomes.
mutable notes: initial AA may be unnecessary
mutable provenance: Lon Chubiz
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: rbs
sequence: https://synbiohub.org/public/igem/BBa_B0075_sequence/1

rbs
type: SequenceAnnotation
persistent identity: annotation1959963
display id: annotation1959963
version: 1
top level: BBa_B0075
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_B0075/annotation1959963/range1959963/1

https://synbiohub.org/public/igem/BBa_B0075/annotation1959963/range1959963/1
type: Range
persistent identity: range1959963
display id: range1959963
version: 1
direction: direction/forward
top level: BBa_B0075
start: 1
end: 8
orientation: inline

BBa_K116401
type: ComponentDefinition
persistent identity: BBa K116401
display id: BBa_K116401
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: external phosphate sensing promoter
creator: Chih-Hsien Yang
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:09:32Z
discontinued: false
dominant: false
group u list: _210_
m user id: 0
owner id: 592
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: promoter of phoB in E.coli, an external phosphate regulated promoter which can be activated when encountering phosphate limitation.
mutable notes: The promoter region should contain PhoB binding site
mutable provenance: E. coli K12 genomic DNA
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: PhoB binding site
sequence annotation: -10
sequence annotation: -35
sequence: https://synbiohub.org/public/igem/BBa_K116401_sequence/1

PhoB binding site
type: SequenceAnnotation
persistent identity: annotation1995351
display id: annotation1995351
version: 1
top level: BBa_K116401
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K116401/annotation1995351/range1995351/1

https://synbiohub.org/public/igem/BBa_K116401/annotation1995351/range1995351/1
type: Range
persistent identity: range1995351
display id: range1995351
version: 1
direction: direction/forward
top level: BBa_K116401
start: 420
end: 439
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1995353
display id: annotation1995353
version: 1
top level: BBa_K116401
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K116401/annotation1995353/range1995353/1

https://synbiohub.org/public/igem/BBa_K116401/annotation1995353/range1995353/1
type: Range
persistent identity: range1995353
display id: range1995353
version: 1
direction: direction/forward
top level: BBa_K116401
start: 448
end: 455
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1995352
display id: annotation1995352
version: 1
top level: BBa_K116401
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K116401/annotation1995352/range1995352/1

https://synbiohub.org/public/igem/BBa_K116401/annotation1995352/range1995352/1
type: Range
persistent identity: range1995352
display id: range1995352
version: 1
direction: direction/forward
top level: BBa_K116401
start: 421
end: 429
orientation: inline

BBa_B0072
type: ComponentDefinition
persistent identity: BBa B0072
display id: BBa_B0072
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: orthogonal RBS
creator: Bartholomew Canton
created: 2006-05-01T11:00:00Z
modified: 2015-08-31T04:07:21Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_6_
m user id: 0
owner id: 135
owning group id: 6
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_B0072_sequence/1

pJEJAM19
type: ComponentDefinition
persistent identity: BBa K678067
display id: BBa_K678067
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pJEJAM19
creator: DTU-Denmark-2
created: 2011-09-12T11:00:00Z
modified: 2015-05-08T01:13:05Z
discontinued: false
dominant: false
experience: experience/None
group u list: _882_
m user id: 0
owner id: 8341
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: To be announced
mutable notes: To be announced
mutable provenance: To be announced
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000155
role: partType/Plasmid
component: BBa_K678032
component: BBa_K678046
component: BBa_K678041
component: BBa_K678039
component: BBa_K678031
component: BBa_K678001
sequence annotation: BBa_K678046
sequence annotation: BBa_K678001
sequence annotation: BBa_K678039
sequence annotation: BBa_K678041
sequence annotation: BBa_K678032
sequence annotation: BBa_K678031
sequence: https://synbiohub.org/public/igem/BBa_K678067_sequence/1

BBa_K678032
type: Component
persistent identity: component2127615
display id: component2127615
version: 1
top level: pJEJAM19
access: public
definition: mRFP1

BBa_K678046
type: Component
persistent identity: component2127624
display id: component2127624
version: 1
top level: pJEJAM19
access: public
definition: pFun

BBa_K678041
type: Component
persistent identity: component2127621
display id: component2127621
version: 1
top level: pJEJAM19
access: public
definition: BBa_K678041

BBa_K678039
type: Component
persistent identity: component2127618
display id: component2127618
version: 1
top level: pJEJAM19
access: public
definition: BBa_K678039

BBa_K678031
type: Component
persistent identity: component2127612
display id: component2127612
version: 1
top level: pJEJAM19
access: public
definition: mRFP1

BBa_K678001
type: Component
persistent identity: component2127609
display id: component2127609
version: 1
top level: pJEJAM19
access: public
definition: PalcA

BBa_K678046
type: SequenceAnnotation
persistent identity: annotation2127624
display id: annotation2127624
version: 1
top level: pJEJAM19
location: https://synbiohub.org/public/igem/BBa_K678067/annotation2127624/range2127624/1
component: BBa_K678046

https://synbiohub.org/public/igem/BBa_K678067/annotation2127624/range2127624/1
type: Range
persistent identity: range2127624
display id: range2127624
version: 1
direction: direction/reverse
top level: pJEJAM19
start: 5311
end: 8188
orientation: inline

BBa_K678001
type: SequenceAnnotation
persistent identity: annotation2127609
display id: annotation2127609
version: 1
top level: pJEJAM19
location: https://synbiohub.org/public/igem/BBa_K678067/annotation2127609/range2127609/1
component: BBa_K678001

https://synbiohub.org/public/igem/BBa_K678067/annotation2127609/range2127609/1
type: Range
persistent identity: range2127609
display id: range2127609
version: 1
direction: direction/reverse
top level: pJEJAM19
start: 1
end: 843
orientation: inline

BBa_K678039
type: SequenceAnnotation
persistent identity: annotation2127618
display id: annotation2127618
version: 1
top level: pJEJAM19
location: https://synbiohub.org/public/igem/BBa_K678067/annotation2127618/range2127618/1
component: BBa_K678039

https://synbiohub.org/public/igem/BBa_K678067/annotation2127618/range2127618/1
type: Range
persistent identity: range2127618
display id: range2127618
version: 1
direction: direction/reverse
top level: pJEJAM19
start: 2238
end: 3126
orientation: inline

BBa_K678041
type: SequenceAnnotation
persistent identity: annotation2127621
display id: annotation2127621
version: 1
top level: pJEJAM19
location: https://synbiohub.org/public/igem/BBa_K678067/annotation2127621/range2127621/1
component: BBa_K678041

https://synbiohub.org/public/igem/BBa_K678067/annotation2127621/range2127621/1
type: Range
persistent identity: range2127621
display id: range2127621
version: 1
direction: direction/reverse
top level: pJEJAM19
start: 3135
end: 5302
orientation: inline

BBa_K678032
type: SequenceAnnotation
persistent identity: annotation2127615
display id: annotation2127615
version: 1
top level: pJEJAM19
location: https://synbiohub.org/public/igem/BBa_K678067/annotation2127615/range2127615/1
component: BBa_K678032

https://synbiohub.org/public/igem/BBa_K678067/annotation2127615/range2127615/1
type: Range
persistent identity: range2127615
display id: range2127615
version: 1
direction: direction/reverse
top level: pJEJAM19
start: 1543
end: 2229
orientation: inline

BBa_K678031
type: SequenceAnnotation
persistent identity: annotation2127612
display id: annotation2127612
version: 1
top level: pJEJAM19
location: https://synbiohub.org/public/igem/BBa_K678067/annotation2127612/range2127612/1
component: BBa_K678031

https://synbiohub.org/public/igem/BBa_K678067/annotation2127612/range2127612/1
type: Range
persistent identity: range2127612
display id: range2127612
version: 1
direction: direction/reverse
top level: pJEJAM19
start: 852
end: 1534
orientation: inline

BBa_J34803
type: ComponentDefinition
persistent identity: BBa J34803
display id: BBa_J34803
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: ribosome binding site
creator: Alexandra Choutko
created: 2006-10-25T11:00:00Z
modified: 2015-08-31T04:08:46Z
discontinued: false
dominant: false
experience: experience/None
group u list: _62_
m user id: 0
owner id: 1085
owning group id: 62
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: a
mutable notes: a
mutable provenance: a
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J34803_sequence/1

BBa_K1777019
type: ComponentDefinition
persistent identity: BBa K1777019
display id: BBa_K1777019
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: flexible linker
creator: Xuhang Li
created: 2015-09-17T11:00:00Z
modified: 2015-09-20T02:06:57Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2199_
m user id: 27798
owner id: 25645
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This a flexible linker combined by two former linkers, which can be easily connect to the end of protein with overlap pcr.
mutable notes: when we chose a linker for our fusion protein，we would have wanted to use -gggsgggs- linker. But we found that such a tandem repeating linker is hard to work well with overlap PCR because the OE region should be unique. Therefore, we improved the original part by change the first GGGS to a carefully selected natural linker from linker database to avoid repeating sequence. Besides, we used the selected natural linker to adjust its length to about 50A.
mutable provenance: BBa_K1486003
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: natural linker
sequence annotation: GS linker
sequence: https://synbiohub.org/public/igem/BBa_K1777019_sequence/1

natural linker
type: SequenceAnnotation
persistent identity: annotation2476148
display id: annotation2476148
version: 1
top level: BBa_K1777019
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1777019/annotation2476148/range2476148/1

https://synbiohub.org/public/igem/BBa_K1777019/annotation2476148/range2476148/1
type: Range
persistent identity: range2476148
display id: range2476148
version: 1
direction: direction/forward
top level: BBa_K1777019
start: 1
end: 21
orientation: inline

GS linker
type: SequenceAnnotation
persistent identity: annotation2476149
display id: annotation2476149
version: 1
top level: BBa_K1777019
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1777019/annotation2476149/range2476149/1

https://synbiohub.org/public/igem/BBa_K1777019/annotation2476149/range2476149/1
type: Range
persistent identity: range2476149
display id: range2476149
version: 1
direction: direction/forward
top level: BBa_K1777019
start: 22
end: 36
orientation: inline

BBa_Z0252
type: ComponentDefinition
persistent identity: BBa Z0252
display id: BBa_Z0252
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: T7 weak binding and processivity
creator: T7.2
created: 2005-04-04T11:00:00Z
modified: 2015-05-08T01:14:55Z
discontinued: false
dominant: false
experience: experience/None
group u list: _10_
m user id: 0
owner id: 250
owning group id: 10
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Promoter for T7 RNA polymerase
mutable provenance: T7
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: 23bp conserved region
sequence annotation: t -> a mutation from consensus
sequence annotation: tga -> acg mutation from consensus
sequence: https://synbiohub.org/public/igem/BBa_Z0252_sequence/1

23bp conserved region
type: SequenceAnnotation
persistent identity: annotation1476343
display id: annotation1476343
version: 1
top level: BBa_Z0252
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_Z0252/annotation1476343/range1476343/1

https://synbiohub.org/public/igem/BBa_Z0252/annotation1476343/range1476343/1
type: Range
persistent identity: range1476343
display id: range1476343
version: 1
direction: direction/forward
top level: BBa_Z0252
start: 6
end: 28
orientation: inline

t -> a mutation from consensus
type: SequenceAnnotation
persistent identity: annotation1476345
display id: annotation1476345
version: 1
top level: BBa_Z0252
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_Z0252/annotation1476345/range1476345/1

https://synbiohub.org/public/igem/BBa_Z0252/annotation1476345/range1476345/1
type: Range
persistent identity: range1476345
display id: range1476345
version: 1
direction: direction/forward
top level: BBa_Z0252
start: 21
end: 21
orientation: inline

tga -> acg mutation from consensus
type: SequenceAnnotation
persistent identity: annotation1476344
display id: annotation1476344
version: 1
top level: BBa_Z0252
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_Z0252/annotation1476344/range1476344/1

https://synbiohub.org/public/igem/BBa_Z0252/annotation1476344/range1476344/1
type: Range
persistent identity: range1476344
display id: range1476344
version: 1
direction: direction/forward
top level: BBa_Z0252
start: 10
end: 12
orientation: inline

BBa_J64750
type: ComponentDefinition
persistent identity: BBa J64750
display id: BBa_J64750
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: SPI-1 TTSS secretion-linked promoter from Salmonella
creator: Jeffrey J Tabor
created: 2007-05-14T11:00:00Z
modified: 2015-05-08T01:08:17Z
discontinued: false
dominant: false
experience: experience/None
group u list: _98_
m user id: 0
owner id: 88
owning group id: 98
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This promoter is activated when functional SPI-1 type III secretion systems are present and functional in Salmonella typhimurium 1344.
mutable notes: The promoter includes the binding site for the transcription factor InvF. This transcription factor is activated by the SicA chaparone when the secretion system is active.
mutable provenance: This part was cloned from Salmonella typhimurium 1344 genomic DNA.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: wt rbs
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_J64750_sequence/1

wt rbs
type: SequenceAnnotation
persistent identity: annotation1932768
display id: annotation1932768
version: 1
top level: BBa_J64750
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_J64750/annotation1932768/range1932768/1

https://synbiohub.org/public/igem/BBa_J64750/annotation1932768/range1932768/1
type: Range
persistent identity: range1932768
display id: range1932768
version: 1
direction: direction/forward
top level: BBa_J64750
start: 153
end: 158
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation1932769
display id: annotation1932769
version: 1
top level: BBa_J64750
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_J64750/annotation1932769/range1932769/1

https://synbiohub.org/public/igem/BBa_J64750/annotation1932769/range1932769/1
type: Range
persistent identity: range1932769
display id: range1932769
version: 1
direction: direction/forward
top level: BBa_J64750
start: 165
end: 167
orientation: inline

BBa_J23105
type: ComponentDefinition
persistent identity: BBa J23105
display id: BBa_J23105
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: constitutive promoter family member
creator: John Anderson
created: 2006-08-13T11:00:00Z
modified: 2015-08-31T04:08:40Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: true
mutable description: Later
mutable notes: N/A
mutable provenance: Isolated from library of promoters
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J23105_sequence/1

BBa_K105030
type: ComponentDefinition
persistent identity: BBa K105030
display id: BBa_K105030
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: cyc28 minimal promoter
creator: Michael Wild
created: 2008-10-25T11:00:00Z
modified: 2015-05-08T01:08:52Z
discontinued: false
dominant: false
experience: experience/None
group u list: _253_
m user id: 0
owner id: 3313
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This is a derivate of BBa_K105027. Due to a point mutation in the TATA box region the efficiency of this minimal promoter is only 28 % of the natural yeast CYC1 promoter.
mutable notes: This BioBrick contains the TATA-box with one point mutation and most of the 5'UTR (-139 to -36, relative to the translational start) of the natural CYC1 promoter. A Kozak sequence is not included and has thus to be added! Even if there isn't any need for this BioBrick to be fusible to other protein domains, we stayed with our design.
mutable provenance: Amplification and Mutation from the plasmid pAH3
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: TATA-box
sequence annotation: A -> T
sequence: https://synbiohub.org/public/igem/BBa_K105030_sequence/1

TATA-box
type: SequenceAnnotation
persistent identity: annotation1989839
display id: annotation1989839
version: 1
top level: BBa_K105030
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K105030/annotation1989839/range1989839/1

https://synbiohub.org/public/igem/BBa_K105030/annotation1989839/range1989839/1
type: Range
persistent identity: range1989839
display id: range1989839
version: 1
direction: direction/forward
top level: BBa_K105030
start: 17
end: 23
orientation: inline

A -> T
type: SequenceAnnotation
persistent identity: annotation1989840
display id: annotation1989840
version: 1
top level: BBa_K105030
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_K105030/annotation1989840/range1989840/1

https://synbiohub.org/public/igem/BBa_K105030/annotation1989840/range1989840/1
type: Range
persistent identity: range1989840
display id: range1989840
version: 1
direction: direction/forward
top level: BBa_K105030
start: 22
end: 22
orientation: inline

LacI
type: ComponentDefinition
persistent identity: BBa R0010
display id: BBa_R0010
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: promoter (lacI regulated)
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) The pLac regulatory region is a 243 base-pair sequence with standard BioBrick prefix and suffix sections on its ends. It contains two protein binding sites: CAP, which is generally present in E.coli and is assocciated with cell health and availability of glucose., and LacI, the Lac inhibitor <bb_part>BBa_C0010</bb_part> which binds in an dimerized cooperative manner to inhibit the transcription of the protein that follows. In the presence of lactose or IPTG, an analog of lactose, LacI is unable to correctly bind and inhibit transcription. This allows <bb_part>BBa_R0010</bb_part> to be used as a inverter or as a detector of lactose or IPTG.
mutable notes: LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and this regulator. This part is incompatible with environments containing lactose or lactose analogs.
mutable provenance: The Plac insert was PCR'd from the MG1655 strain of E.coli K12.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: start
sequence annotation: end of LacI coding region (inactive)
sequence annotation: CAP binding site
sequence annotation: BBa_R0010
sequence annotation: -10
sequence annotation: LacI binding site
sequence annotation: -35
sequence: https://synbiohub.org/public/igem/BBa_R0010_sequence/1

start
type: SequenceAnnotation
persistent identity: annotation1961227
display id: annotation1961227
version: 1
top level: LacI
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961227/range1961227/1

https://synbiohub.org/public/igem/BBa_R0010/annotation1961227/range1961227/1
type: Range
persistent identity: range1961227
display id: range1961227
version: 1
direction: direction/forward
top level: LacI
start: 173
end: 173
orientation: inline

end of LacI coding region (inactive)
type: SequenceAnnotation
persistent identity: annotation1961221
display id: annotation1961221
version: 1
top level: LacI
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961221/range1961221/1

https://synbiohub.org/public/igem/BBa_R0010/annotation1961221/range1961221/1
type: Range
persistent identity: range1961221
display id: range1961221
version: 1
direction: direction/forward
top level: LacI
start: 1
end: 88
orientation: inline

CAP binding site
type: SequenceAnnotation
persistent identity: annotation1961223
display id: annotation1961223
version: 1
top level: LacI
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961223/range1961223/1

https://synbiohub.org/public/igem/BBa_R0010/annotation1961223/range1961223/1
type: Range
persistent identity: range1961223
display id: range1961223
version: 1
direction: direction/forward
top level: LacI
start: 89
end: 126
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation1961222
display id: annotation1961222
version: 1
top level: LacI
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961222/range1961222/1

https://synbiohub.org/public/igem/BBa_R0010/annotation1961222/range1961222/1
type: Range
persistent identity: range1961222
display id: range1961222
version: 1
direction: direction/forward
top level: LacI
start: 1
end: 200
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1961225
display id: annotation1961225
version: 1
top level: LacI
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961225/range1961225/1

https://synbiohub.org/public/igem/BBa_R0010/annotation1961225/range1961225/1
type: Range
persistent identity: range1961225
display id: range1961225
version: 1
direction: direction/forward
top level: LacI
start: 161
end: 166
orientation: inline

LacI binding site
type: SequenceAnnotation
persistent identity: annotation1961226
display id: annotation1961226
version: 1
top level: LacI
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961226/range1961226/1

https://synbiohub.org/public/igem/BBa_R0010/annotation1961226/range1961226/1
type: Range
persistent identity: range1961226
display id: range1961226
version: 1
direction: direction/forward
top level: LacI
start: 166
end: 200
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1961224
display id: annotation1961224
version: 1
top level: LacI
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961224/range1961224/1

https://synbiohub.org/public/igem/BBa_R0010/annotation1961224/range1961224/1
type: Range
persistent identity: range1961224
display id: range1961224
version: 1
direction: direction/forward
top level: LacI
start: 137
end: 142
orientation: inline

BBa_K112707
type: ComponentDefinition
persistent identity: BBa K112707
display id: BBa_K112707
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Pspv from Salmonella
creator: Bing Xia
created: 2008-10-20T11:00:00Z
modified: 2015-05-08T01:09:18Z
discontinued: false
dominant: false
experience: experience/None
group u list: _224_
m user id: 0
owner id: 2998
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: The spv promoter. ---- This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at: [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
mutable provenance: Construction of <Pspv> basic part K112707 PCR of dv017/dv016 on pBca1037 (499bp, EcoRI/BamHI/DpnI) Sub into pBca1256 (EcoRI/BamHI, 2472+499, L) Product is pBca1256-K112707 ----------------------------------------------- dv017 Forward biobricking of <Pspv> cgataGAATTCatgAGATCTaGATCCTGTGATGTTTGGCG dv016 Reverse biobricking of <Pspv> cgttaGGATCCGATAATGTtTGCAGGGGAATTATTTTG
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K112707_sequence/1

BBa_I764001
type: ComponentDefinition
persistent identity: BBa I764001
display id: BBa_I764001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Ethanol regulated promoter AOX1
creator: Dave Westenberg
created: 2007-08-28T11:00:00Z
modified: 2015-08-31T04:08:09Z
discontinued: false
dominant: false
group u list: _137_
m user id: 0
owner id: 1818
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Needs to be added.
mutable notes: No special considerations.
mutable provenance: Pichia pastoris genome.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: primer_binding
sequence annotation: BioBrick
sequence: https://synbiohub.org/public/igem/BBa_I764001_sequence/1

primer_binding
type: SequenceAnnotation
persistent identity: annotation1943749
display id: annotation1943749
version: 1
top level: BBa_I764001
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_I764001/annotation1943749/range1943749/1

https://synbiohub.org/public/igem/BBa_I764001/annotation1943749/range1943749/1
type: Range
persistent identity: range1943749
display id: range1943749
version: 1
direction: direction/forward
top level: BBa_I764001
start: 1
end: 19
orientation: inline

BioBrick
type: SequenceAnnotation
persistent identity: annotation1943748
display id: annotation1943748
version: 1
top level: BBa_I764001
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_I764001/annotation1943748/range1943748/1

https://synbiohub.org/public/igem/BBa_I764001/annotation1943748/range1943748/1
type: Range
persistent identity: range1943748
display id: range1943748
version: 1
direction: direction/forward
top level: BBa_I764001
start: 1
end: 867
orientation: inline

primer_binding
type: SequenceAnnotation
persistent identity: annotation1943750
display id: annotation1943750
version: 1
top level: BBa_I764001
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_I764001/annotation1943750/range1943750/1

https://synbiohub.org/public/igem/BBa_I764001/annotation1943750/range1943750/1
type: Range
persistent identity: range1943750
display id: range1943750
version: 1
direction: direction/reverse
top level: BBa_I764001
start: 844
end: 867
orientation: inline

BBa_K131001
type: ComponentDefinition
persistent identity: BBa K131001
display id: BBa_K131001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: ColE2 SOS Promoter
creator: Kevin McLeod
created: 2008-07-21T11:00:00Z
modified: 2015-05-08T01:09:47Z
discontinued: true
dominant: false
experience: experience/None
group u list: _259_
m user id: 0
owner id: 1917
owning group id: 9
sample status: Discontinued
status: status/Deleted
bookmark: false
mutable description: This promoter normally represses the ColE2 activity gene, ceaB, in the ColE2 operon. To activate this promoter would require a RecA positive strain, as the protein LexA represses the promoter. To induce this promoter, an intercalating agent, mitomycin C, was utilized to induce the SOS response, and thus the expression of the genes under control of this promoter.
mutable notes: Lab strains of E. coli, such as Top10 from Invitrogen, are deficient in RecA so we required a RecA positive strain to induce this promoter. We used BL21 from Novagen.
mutable provenance: This part comes from the ColE2 operon, which came from a plasmid.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: SOS Box
sequence annotation: Region of Dyad Symmetry
sequence annotation: -15 Signal
sequence annotation: -35 Signal
sequence: https://synbiohub.org/public/igem/BBa_K131001_sequence/1

SOS Box
type: SequenceAnnotation
persistent identity: annotation1968167
display id: annotation1968167
version: 1
top level: BBa_K131001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K131001/annotation1968167/range1968167/1

https://synbiohub.org/public/igem/BBa_K131001/annotation1968167/range1968167/1
type: Range
persistent identity: range1968167
display id: range1968167
version: 1
direction: direction/forward
top level: BBa_K131001
start: 252
end: 267
orientation: inline

Region of Dyad Symmetry
type: SequenceAnnotation
persistent identity: annotation1968166
display id: annotation1968166
version: 1
top level: BBa_K131001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K131001/annotation1968166/range1968166/1

https://synbiohub.org/public/igem/BBa_K131001/annotation1968166/range1968166/1
type: Range
persistent identity: range1968166
display id: range1968166
version: 1
direction: direction/forward
top level: BBa_K131001
start: 136
end: 161
orientation: inline

-15 Signal
type: SequenceAnnotation
persistent identity: annotation1968165
display id: annotation1968165
version: 1
top level: BBa_K131001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K131001/annotation1968165/range1968165/1

https://synbiohub.org/public/igem/BBa_K131001/annotation1968165/range1968165/1
type: Range
persistent identity: range1968165
display id: range1968165
version: 1
direction: direction/forward
top level: BBa_K131001
start: 241
end: 246
orientation: inline

-35 Signal
type: SequenceAnnotation
persistent identity: annotation1968164
display id: annotation1968164
version: 1
top level: BBa_K131001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K131001/annotation1968164/range1968164/1

https://synbiohub.org/public/igem/BBa_K131001/annotation1968164/range1968164/1
type: Range
persistent identity: range1968164
display id: range1968164
version: 1
direction: direction/forward
top level: BBa_K131001
start: 218
end: 224
orientation: inline

BBa_J05218
type: ComponentDefinition
persistent identity: BBa J05218
display id: BBa_J05218
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Regulator for R4-cMaf
creator: Alexander Roth
created: 2005-09-07T11:00:00Z
modified: 2015-08-31T04:08:15Z
discontinued: false
dominant: false
group u list: _34_
m user id: 0
owner id: 412
owning group id: 34
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: binding sites for R1-CREBH and R2-YAP7
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J05218_sequence/1

BBa_K1824894
type: ComponentDefinition
persistent identity: BBa K1824894
display id: BBa_K1824894
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Plux + RBS
creator: Zixu Wang
created: 2015-09-09T11:00:00Z
modified: 2015-09-11T08:44:29Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2250_
m user id: 26000
owner id: 26000
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This composite regulatory part can be repressed by LuxR and activated by LuxR-3OC6HSL. Induced Plux is a strong promoter and B0034 is a strong RBS, which makes it can be used for both transcription and translation.
mutable notes: No special concern
mutable provenance: Plux is from V. fischeri and B0034 is based on Elowitz repressilator.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1824889
component: BBa_B0034
component: BBa_K1824890
component: BBa_R0062
sequence annotation: BBa_B0034
sequence annotation: BBa_K1824890
sequence annotation: BBa_R0062
sequence annotation: BBa_K1824889
sequence: https://synbiohub.org/public/igem/BBa_K1824894_sequence/1

BBa_K1824889
type: Component
persistent identity: component2450476
display id: component2450476
version: 1
top level: BBa_K1824894
access: public
definition: https://synbiohub.org/public/igem/BBa_K1824889/1

BBa_B0034
type: Component
persistent identity: component2450478
display id: component2450478
version: 1
top level: BBa_K1824894
access: public
definition: BBa_B0034

BBa_K1824890
type: Component
persistent identity: component2450479
display id: component2450479
version: 1
top level: BBa_K1824894
access: public
definition: https://synbiohub.org/public/igem/BBa_K1824890/1

BBa_R0062
type: Component
persistent identity: component2450471
display id: component2450471
version: 1
top level: BBa_K1824894
access: public
definition: lux pR

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2450478
display id: annotation2450478
version: 1
top level: BBa_K1824894
location: https://synbiohub.org/public/igem/BBa_K1824894/annotation2450478/range2450478/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1824894/annotation2450478/range2450478/1
type: Range
persistent identity: range2450478
display id: range2450478
version: 1
direction: direction/reverse
top level: BBa_K1824894
start: 64
end: 75
orientation: inline

BBa_K1824890
type: SequenceAnnotation
persistent identity: annotation2450479
display id: annotation2450479
version: 1
top level: BBa_K1824894
location: https://synbiohub.org/public/igem/BBa_K1824894/annotation2450479/range2450479/1
component: BBa_K1824890

https://synbiohub.org/public/igem/BBa_K1824894/annotation2450479/range2450479/1
type: Range
persistent identity: range2450479
display id: range2450479
version: 1
direction: direction/reverse
top level: BBa_K1824894
start: 76
end: 81
orientation: inline

BBa_R0062
type: SequenceAnnotation
persistent identity: annotation2450471
display id: annotation2450471
version: 1
top level: BBa_K1824894
location: https://synbiohub.org/public/igem/BBa_K1824894/annotation2450471/range2450471/1
component: BBa_R0062

https://synbiohub.org/public/igem/BBa_K1824894/annotation2450471/range2450471/1
type: Range
persistent identity: range2450471
display id: range2450471
version: 1
direction: direction/forward
top level: BBa_K1824894
start: 1
end: 55
orientation: inline

BBa_K1824889
type: SequenceAnnotation
persistent identity: annotation2450476
display id: annotation2450476
version: 1
top level: BBa_K1824894
location: https://synbiohub.org/public/igem/BBa_K1824894/annotation2450476/range2450476/1
component: BBa_K1824889

https://synbiohub.org/public/igem/BBa_K1824894/annotation2450476/range2450476/1
type: Range
persistent identity: range2450476
display id: range2450476
version: 1
direction: direction/reverse
top level: BBa_K1824894
start: 56
end: 63
orientation: inline

BBa_K1585118
type: ComponentDefinition
persistent identity: BBa K1585118
display id: BBa_K1585118
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Anderson Promoter with lacI binding site
creator: Tobias Schwanemann
created: 2015-08-31T11:00:00Z
modified: 2015-09-01T02:31:42Z
discontinued: false
dominant: false
experience: experience/Fails
group u list: _2002_
m user id: 24474
owner id: 24474
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Promoters of the Anderson Promoter Collection with lacI binding site derived from [http://parts.igem.org/Part:BBa_R0010 R_0010] to make them [http://openwetware.org/wiki/IPTG IPTG] iducible.
mutable notes: We choosed lacI-binding-site of part BBa_R0010 because we assumed older parts would be more reliable. The part is shipped in backbone [http://parts.igem.org/Part:BBa_J61002 J61002] because the functionality is directly visible by adding IPTG. RFP ([http://parts.igem.org/Part:BBa_E1010 E1010]) should get expressed then.
mutable provenance: Assambly of [http://parts.igem.org/Part:BBa_J23118 J23118] and lacI-binding-site of [http://parts.igem.org/Part:BBa_R0010 R_0010] The "lacI binding site" of Part [http://parts.igem.org/Part:BBa_R0010 R_0010] differs from lacO from Part [http://parts.igem.org/Part:BBa_J33207 BBa_J33207].
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
component: BBa_J23118
component: BBa_K1585999
sequence annotation: BBa_J23118
sequence annotation: BBa_K1585999
sequence: https://synbiohub.org/public/igem/BBa_K1585118_sequence/1

BBa_J23118
type: Component
persistent identity: component2441715
display id: component2441715
version: 1
top level: BBa_K1585118
access: public
definition: BBa_J23118

BBa_K1585999
type: Component
persistent identity: component2441717
display id: component2441717
version: 1
top level: BBa_K1585118
access: public
definition: https://synbiohub.org/public/igem/BBa_K1585999/1

BBa_J23118
type: SequenceAnnotation
persistent identity: annotation2441715
display id: annotation2441715
version: 1
top level: BBa_K1585118
location: https://synbiohub.org/public/igem/BBa_K1585118/annotation2441715/range2441715/1
component: BBa_J23118

https://synbiohub.org/public/igem/BBa_K1585118/annotation2441715/range2441715/1
type: Range
persistent identity: range2441715
display id: range2441715
version: 1
direction: direction/reverse
top level: BBa_K1585118
start: 1
end: 35
orientation: inline

BBa_K1585999
type: SequenceAnnotation
persistent identity: annotation2441717
display id: annotation2441717
version: 1
top level: BBa_K1585118
location: https://synbiohub.org/public/igem/BBa_K1585118/annotation2441717/range2441717/1
component: BBa_K1585999

https://synbiohub.org/public/igem/BBa_K1585118/annotation2441717/range2441717/1
type: Range
persistent identity: range2441717
display id: range2441717
version: 1
direction: direction/reverse
top level: BBa_K1585118
start: 44
end: 78
orientation: inline

BBa_J61143
type: ComponentDefinition
persistent identity: BBa J61143
display id: BBa_J61143
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: {rbs1} Library Member in BBb
creator: John Anderson
created: 2007-09-03T11:00:00Z
modified: 2015-08-31T01:56:25Z
discontinued: false
dominant: false
experience: experience/None
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: {{BBbRBS1Family}}
mutable notes: {{BerkiGEM2007-Biobrick2.0Intro}}
mutable provenance: Synthetic
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J61143_sequence/1

BBa_K086020
type: ComponentDefinition
persistent identity: BBa K086020
display id: BBa_K086020
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: modified Lutz-Bujard LacO promoter,with alternative sigma factor σ24
creator: Gairik Sachdeva
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:08:36Z
discontinued: false
dominant: false
group u list: _197_
m user id: 0
owner id: 2578
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: modified Lutz-Bujard LacO promoter,with alternative sigma factor σ24
mutable notes: Cleaned
mutable provenance: Lac O and sigma 24 binding conserved sequences
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: P24_3
sequence: https://synbiohub.org/public/igem/BBa_K086020_sequence/1

P24_3
type: SequenceAnnotation
persistent identity: annotation1995754
display id: annotation1995754
version: 1
top level: BBa_K086020
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K086020/annotation1995754/range1995754/1

https://synbiohub.org/public/igem/BBa_K086020/annotation1995754/range1995754/1
type: Range
persistent identity: range1995754
display id: range1995754
version: 1
direction: direction/forward
top level: BBa_K086020
start: 1
end: 55
orientation: inline

RhI
type: ComponentDefinition
persistent identity: BBa I14017
display id: BBa_I14017
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: P(Rhl)
creator: Vikram Vijayan, Allen Hsu, Lawrence Fomundam
created: 2004-08-01T11:00:00Z
modified: 2015-08-31T04:07:37Z
discontinued: false
dominant: true
experience: experience/None
group u list: _4_
m user id: 0
owner id: 171
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: RhlR and C4HSL regulated promoter.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -35
sequence annotation: modified P(Rhl)
sequence annotation: RhlR
sequence: https://synbiohub.org/public/igem/BBa_I14017_sequence/1

-35
type: SequenceAnnotation
persistent identity: annotation1043007
display id: annotation1043007
version: 1
top level: RhI
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I14017/annotation1043007/range1043007/1

https://synbiohub.org/public/igem/BBa_I14017/annotation1043007/range1043007/1
type: Range
persistent identity: range1043007
display id: range1043007
version: 1
direction: direction/forward
top level: RhI
start: 21
end: 50
orientation: inline

modified P(Rhl)
type: SequenceAnnotation
persistent identity: annotation1043006
display id: annotation1043006
version: 1
top level: RhI
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_I14017/annotation1043006/range1043006/1

https://synbiohub.org/public/igem/BBa_I14017/annotation1043006/range1043006/1
type: Range
persistent identity: range1043006
display id: range1043006
version: 1
direction: direction/forward
top level: RhI
start: 1
end: 51
orientation: inline

RhlR
type: SequenceAnnotation
persistent identity: annotation1043005
display id: annotation1043005
version: 1
top level: RhI
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I14017/annotation1043005/range1043005/1

https://synbiohub.org/public/igem/BBa_I14017/annotation1043005/range1043005/1
type: Range
persistent identity: range1043005
display id: range1043005
version: 1
direction: direction/forward
top level: RhI
start: 1
end: 20
orientation: inline

BBa_K1486006
type: ComponentDefinition
persistent identity: BBa K1486006
display id: BBa_K1486006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: IFP[1]
creator: iGem EPFL 2014
created: 2014-08-24T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1866_
m user id: 0
owner id: 21033
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is the first part of the split infrared fluorescent protein (IFP1.4). To be used with IFP[2].
mutable notes: When fused to another coding sequence, insert a flexible linker
mutable provenance: From Michnick Article. To Edit
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: STOP
sequence annotation: START
sequence annotation: IFP[1]
sequence: https://synbiohub.org/public/igem/BBa_K1486006_sequence/1

STOP
type: SequenceAnnotation
persistent identity: annotation2418011
display id: annotation2418011
version: 1
top level: BBa_K1486006
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1486006/annotation2418011/range2418011/1

https://synbiohub.org/public/igem/BBa_K1486006/annotation2418011/range2418011/1
type: Range
persistent identity: range2418011
display id: range2418011
version: 1
direction: direction/forward
top level: BBa_K1486006
start: 403
end: 405
orientation: inline

START
type: SequenceAnnotation
persistent identity: annotation2418009
display id: annotation2418009
version: 1
top level: BBa_K1486006
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1486006/annotation2418009/range2418009/1

https://synbiohub.org/public/igem/BBa_K1486006/annotation2418009/range2418009/1
type: Range
persistent identity: range2418009
display id: range2418009
version: 1
direction: direction/forward
top level: BBa_K1486006
start: 1
end: 3
orientation: inline

IFP[1]
type: SequenceAnnotation
persistent identity: annotation2418010
display id: annotation2418010
version: 1
top level: BBa_K1486006
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1486006/annotation2418010/range2418010/1

https://synbiohub.org/public/igem/BBa_K1486006/annotation2418010/range2418010/1
type: Range
persistent identity: range2418010
display id: range2418010
version: 1
direction: direction/forward
top level: BBa_K1486006
start: 1
end: 405
orientation: inline

BBa_K1486017
type: ComponentDefinition
persistent identity: BBa K1486017
display id: BBa_K1486017
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: fLuc[2]
creator: EPFL iGem team 2014
created: 2014-09-14T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is the second part of the split Firefly luciferase. To be used with fLuc[1].
mutable notes: to edit
mutable provenance: to edit
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence: https://synbiohub.org/public/igem/BBa_K1486017_sequence/1

BBa_R2201
type: ComponentDefinition
persistent identity: BBa R2201
display id: BBa_R2201
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: C2006-repressible promoter
creator: Reshma Shetty
created: 2007-08-16T11:00:00Z
modified: 2015-05-08T01:14:16Z
discontinued: false
dominant: false
group u list: _41_
m user id: 0
owner id: 126
owning group id: 162
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is a promoter designed to be repressed by BBa_C2006: a MBP.Zif268.GCN4 homodimer.
mutable notes: The operator site is positioned to increase the steric hindrance between the repressor and RNAP based on comparing the structures of the repressor bound to DNA and RNAP bound to DNA.
mutable provenance: This sequence is derived from BBa_R2000 and the protein structures of Zif268.GCN4(-2) bound to DNA and RNAP bound to DNA.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -10
sequence annotation: -35
sequence annotation: operator
sequence: https://synbiohub.org/public/igem/BBa_R2201_sequence/1

-10
type: SequenceAnnotation
persistent identity: annotation1941587
display id: annotation1941587
version: 1
top level: BBa_R2201
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R2201/annotation1941587/range1941587/1

https://synbiohub.org/public/igem/BBa_R2201/annotation1941587/range1941587/1
type: Range
persistent identity: range1941587
display id: range1941587
version: 1
direction: direction/forward
top level: BBa_R2201
start: 33
end: 38
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1941585
display id: annotation1941585
version: 1
top level: BBa_R2201
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R2201/annotation1941585/range1941585/1

https://synbiohub.org/public/igem/BBa_R2201/annotation1941585/range1941585/1
type: Range
persistent identity: range1941585
display id: range1941585
version: 1
direction: direction/forward
top level: BBa_R2201
start: 10
end: 15
orientation: inline

operator
type: SequenceAnnotation
persistent identity: annotation1941586
display id: annotation1941586
version: 1
top level: BBa_R2201
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_R2201/annotation1941586/range1941586/1

https://synbiohub.org/public/igem/BBa_R2201/annotation1941586/range1941586/1
type: Range
persistent identity: range1941586
display id: range1941586
version: 1
direction: direction/forward
top level: BBa_R2201
start: 14
end: 26
orientation: inline

TtrpC
type: ComponentDefinition
persistent identity: BBa K678036
display id: BBa_K678036
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: trpC terminator for Aspergillus nidulans
creator: DTU-Denmark-2
created: 2011-09-03T11:00:00Z
modified: 2015-05-08T01:13:04Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _882_
m user id: 0
owner id: 8341
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: TtrpC is a terminator of tryptophan synthase of Aspergillus nidulans.
mutable notes: Compatible with the Plug'n'Play assembly standard
mutable provenance: From the plasmid p71 from the Center for Microbial Biotechnology at the Technical University of Denmark plasmid collection.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: TtrpC terminator
sequence annotation: Linker 4
sequence: https://synbiohub.org/public/igem/BBa_K678036_sequence/1

TtrpC terminator
type: SequenceAnnotation
persistent identity: annotation2127404
display id: annotation2127404
version: 1
top level: TtrpC
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K678036/annotation2127404/range2127404/1

https://synbiohub.org/public/igem/BBa_K678036/annotation2127404/range2127404/1
type: Range
persistent identity: range2127404
display id: range2127404
version: 1
direction: direction/forward
top level: TtrpC
start: 9
end: 759
orientation: inline

Linker 4
type: SequenceAnnotation
persistent identity: annotation2127403
display id: annotation2127403
version: 1
top level: TtrpC
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K678036/annotation2127403/range2127403/1

https://synbiohub.org/public/igem/BBa_K678036/annotation2127403/range2127403/1
type: Range
persistent identity: range2127403
display id: range2127403
version: 1
direction: direction/forward
top level: TtrpC
start: 1
end: 8
orientation: inline

BBa_J23080
type: ComponentDefinition
persistent identity: BBa J23080
display id: BBa_J23080
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: b0034 derived RBS to match spacing of J01122
creator: bryan hernandez
created: 2006-09-20T11:00:00Z
modified: 2015-08-31T04:08:40Z
discontinued: false
dominant: false
experience: experience/None
group u list: _52_
m user id: 0
owner id: 936
owning group id: 52
sample status: In stock
status: status/Available
bookmark: false
mutable description: RBS spacing modification to [RBS] such that the spacing between the shine-delgarno and the ORF is the same as it is in J01122.
mutable notes: none
mutable provenance: j01122
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J23080_sequence/1

BBa_K118011
type: ComponentDefinition
persistent identity: BBa K118011
display id: BBa_K118011
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PcstA (glucose-repressible promoter)
creator: Andrew Hall
created: 2008-08-18T11:00:00Z
modified: 2015-05-08T01:09:36Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _192_
m user id: 0
owner id: 3282
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: This is the promoter for the ''Eschaerichia coli'' JM109 ''cstA'' gene. It includes the CRP-binding site and the RNA polymerase-binding site. Low glucose concentration results in increased activity by adenylate cyclase. cAMP binds to the cAMP receptor protein, which, in its bound form, is able to associate with the promoter and promote transcription of the downstream gene. (''cstA'' encodes the carbon starvation protein.)
mutable notes: No special considerations
mutable provenance: ''Eschaerichia coli'' JM109 genomic DNA
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: RNA polymerase binding site
sequence annotation: cAMP receptor protein binding site
sequence: https://synbiohub.org/public/igem/BBa_K118011_sequence/1

RNA polymerase binding site
type: SequenceAnnotation
persistent identity: annotation1972436
display id: annotation1972436
version: 1
top level: BBa_K118011
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K118011/annotation1972436/range1972436/1

https://synbiohub.org/public/igem/BBa_K118011/annotation1972436/range1972436/1
type: Range
persistent identity: range1972436
display id: range1972436
version: 1
direction: direction/forward
top level: BBa_K118011
start: 101
end: 131
orientation: inline

cAMP receptor protein binding site
type: SequenceAnnotation
persistent identity: annotation1972435
display id: annotation1972435
version: 1
top level: BBa_K118011
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K118011/annotation1972435/range1972435/1

https://synbiohub.org/public/igem/BBa_K118011/annotation1972435/range1972435/1
type: Range
persistent identity: range1972435
display id: range1972435
version: 1
direction: direction/forward
top level: BBa_K118011
start: 1
end: 42
orientation: inline

BBa_K165036
type: ComponentDefinition
persistent identity: BBa K165036
display id: BBa_K165036
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Gli1 bs + LexA bs + mCYC promoter
creator: John Szymanski
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:10:56Z
discontinued: false
dominant: false
group u list: _267_
m user id: 0
owner id: 2512
owning group id: 58
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: The Gli1 and LexA transcription factor binding sites on the mCYC minimal promoter (in that order.) Transcription regulation comes from Gli1 and LexA-binding transcription factors.
mutable notes: -
mutable provenance: Constructed in our lab with parts from Caroline Ajo-Franklin and David Drubin.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
component: BBa_K165012
component: BBa_K165031
sequence annotation: BBa_K165031
sequence annotation: BBa_K165012
sequence: https://synbiohub.org/public/igem/BBa_K165036_sequence/1

BBa_K165012
type: Component
persistent identity: component1995510
display id: component1995510
version: 1
top level: BBa_K165036
access: public
definition: https://synbiohub.org/public/igem/BBa_K165012/1

BBa_K165031
type: Component
persistent identity: component1995511
display id: component1995511
version: 1
top level: BBa_K165036
access: public
definition: BBa_K165031

BBa_K165031
type: SequenceAnnotation
persistent identity: annotation1995511
display id: annotation1995511
version: 1
top level: BBa_K165036
location: https://synbiohub.org/public/igem/BBa_K165036/annotation1995511/range1995511/1
component: BBa_K165031

https://synbiohub.org/public/igem/BBa_K165036/annotation1995511/range1995511/1
type: Range
persistent identity: range1995511
display id: range1995511
version: 1
direction: direction/reverse
top level: BBa_K165036
start: 136
end: 538
orientation: inline

BBa_K165012
type: SequenceAnnotation
persistent identity: annotation1995510
display id: annotation1995510
version: 1
top level: BBa_K165036
location: https://synbiohub.org/public/igem/BBa_K165036/annotation1995510/range1995510/1
component: BBa_K165012

https://synbiohub.org/public/igem/BBa_K165036/annotation1995510/range1995510/1
type: Range
persistent identity: range1995510
display id: range1995510
version: 1
direction: direction/reverse
top level: BBa_K165036
start: 1
end: 127
orientation: inline

U049O11+D0
type: ComponentDefinition
persistent identity: BBa I732405
display id: BBa_I732405
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Family Member (U049O11+D062NUL)
creator: Zhan Jian
created: 2007-09-19T11:00:00Z
modified: 2015-08-31T04:07:58Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1572
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: A Double Reporter (LacZ-alpha and GFP-AAV) loaded on pSB3K5. For more information please refer to BBa_I732402.
mutable notes: Might be later edited.
mutable provenance: Might be later edited.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: BBa_B0034
sequence annotation: conserved
sequence annotation: BBa_I732007
sequence annotation: stop
sequence annotation: polya
sequence annotation: stem_loop
sequence annotation: mrfp1
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: lacZ
sequence annotation: barcode
sequence annotation: STOP
sequence annotation: BamHI
sequence annotation: BglII
sequence annotation: BBa_E1010
sequence annotation: BBa_I732006
sequence annotation: BBa_B0010
sequence annotation: START
sequence: https://synbiohub.org/public/igem/BBa_I732405_sequence/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1944414
display id: annotation1944414
version: 1
top level: U049O11+D0
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_I732405/annotation1944414/range1944414/1

https://synbiohub.org/public/igem/BBa_I732405/annotation1944414/range1944414/1
type: Range
persistent identity: range1944414
display id: range1944414
version: 1
direction: direction/reverse
top level: U049O11+D0
start: 288
end: 299
orientation: inline

conserved
type: SequenceAnnotation
persistent identity: annotation1944413
display id: annotation1944413
version: 1
top level: U049O11+D0
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_I732405/annotation1944413/range1944413/1

https://synbiohub.org/public/igem/BBa_I732405/annotation1944413/range1944413/1
type: Range
persistent identity: range1944413
display id: range1944413
version: 1
direction: direction/forward
top level: U049O11+D0
start: 292
end: 295
orientation: inline

BBa_I732007
type: SequenceAnnotation
persistent identity: annotation1944406
display id: annotation1944406
version: 1
top level: U049O11+D0
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_I732405/annotation1944406/range1944406/1

https://synbiohub.org/public/igem/BBa_I732405/annotation1944406/range1944406/1
type: Range
persistent identity: range1944406
display id: range1944406
version: 1
direction: direction/reverse
top level: U049O11+D0
start: 1
end: 19
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1944423
display id: annotation1944423
version: 1
top level: U049O11+D0
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_I732405/annotation1944423/range1944423/1

https://synbiohub.org/public/igem/BBa_I732405/annotation1944423/range1944423/1
type: Range
persistent identity: range1944423
display id: range1944423
version: 1
direction: direction/forward
top level: U049O11+D0
start: 1141
end: 1141
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1944422
display id: annotation1944422
version: 1
top level: U049O11+D0
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_I732405/annotation1944422/range1944422/1

https://synbiohub.org/public/igem/BBa_I732405/annotation1944422/range1944422/1
type: Range
persistent identity: range1944422
display id: range1944422
version: 1
direction: direction/forward
top level: U049O11+D0
start: 1135
end: 1148
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1944408
display id: annotation1944408
version: 1
top level: U049O11+D0
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_I732405/annotation1944408/range1944408/1

https://synbiohub.org/public/igem/BBa_I732405/annotation1944408/range1944408/1
type: Range
persistent identity: range1944408
display id: range1944408
version: 1
direction: direction/reverse
top level: U049O11+D0
start: 28
end: 39
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation1944419
display id: annotation1944419
version: 1
top level: U049O11+D0
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_I732405/annotation1944419/range1944419/1

https://synbiohub.org/public/igem/BBa_I732405/annotation1944419/range1944419/1
type: Range
persistent identity: range1944419
display id: range1944419
version: 1
direction: direction/forward
top level: U049O11+D0
start: 1031
end: 1074
orientation: inline

mrfp1
type: SequenceAnnotation
persistent identity: annotation1944415
display id: annotation1944415
version: 1
top level: U049O11+D0
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_I732405/annotation1944415/range1944415/1

https://synbiohub.org/public/igem/BBa_I732405/annotation1944415/range1944415/1
type: Range
persistent identity: range1944415
display id: range1944415
version: 1
direction: direction/forward
top level: U049O11+D0
start: 306
end: 980
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1944420
display id: annotation1944420
version: 1
top level: U049O11+D0
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_I732405/annotation1944420/range1944420/1

https://synbiohub.org/public/igem/BBa_I732405/annotation1944420/range1944420/1
type: Range
persistent identity: range1944420
display id: range1944420
version: 1
direction: direction/forward
top level: U049O11+D0
start: 1108
end: 1148
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1944421
display id: annotation1944421
version: 1
top level: U049O11+D0
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_I732405/annotation1944421/range1944421/1

https://synbiohub.org/public/igem/BBa_I732405/annotation1944421/range1944421/1
type: Range
persistent identity: range1944421
display id: range1944421
version: 1
direction: direction/forward
top level: U049O11+D0
start: 1115
end: 1134
orientation: inline

lacZ
type: SequenceAnnotation
persistent identity: annotation1944410
display id: annotation1944410
version: 1
top level: U049O11+D0
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_I732405/annotation1944410/range1944410/1

https://synbiohub.org/public/igem/BBa_I732405/annotation1944410/range1944410/1
type: Range
persistent identity: range1944410
display id: range1944410
version: 1
direction: direction/forward
top level: U049O11+D0
start: 46
end: 279
orientation: inline

conserved
type: SequenceAnnotation
persistent identity: annotation1944407
display id: annotation1944407
version: 1
top level: U049O11+D0
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_I732405/annotation1944407/range1944407/1

https://synbiohub.org/public/igem/BBa_I732405/annotation1944407/range1944407/1
type: Range
persistent identity: range1944407
display id: range1944407
version: 1
direction: direction/forward
top level: U049O11+D0
start: 32
end: 35
orientation: inline

barcode
type: SequenceAnnotation
persistent identity: annotation1944417
display id: annotation1944417
version: 1
top level: U049O11+D0
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_I732405/annotation1944417/range1944417/1

https://synbiohub.org/public/igem/BBa_I732405/annotation1944417/range1944417/1
type: Range
persistent identity: range1944417
display id: range1944417
version: 1
direction: direction/reverse
top level: U049O11+D0
start: 987
end: 1011
orientation: inline

STOP
type: SequenceAnnotation
persistent identity: annotation1944411
display id: annotation1944411
version: 1
top level: U049O11+D0
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_I732405/annotation1944411/range1944411/1

https://synbiohub.org/public/igem/BBa_I732405/annotation1944411/range1944411/1
type: Range
persistent identity: range1944411
display id: range1944411
version: 1
direction: direction/forward
top level: U049O11+D0
start: 274
end: 279
orientation: inline

BamHI
type: SequenceAnnotation
persistent identity: annotation1944405
display id: annotation1944405
version: 1
top level: U049O11+D0
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I732405/annotation1944405/range1944405/1

https://synbiohub.org/public/igem/BBa_I732405/annotation1944405/range1944405/1
type: Range
persistent identity: range1944405
display id: range1944405
version: 1
direction: direction/forward
top level: U049O11+D0
start: 12
end: 17
orientation: inline

BglII
type: SequenceAnnotation
persistent identity: annotation1944404
display id: annotation1944404
version: 1
top level: U049O11+D0
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I732405/annotation1944404/range1944404/1

https://synbiohub.org/public/igem/BBa_I732405/annotation1944404/range1944404/1
type: Range
persistent identity: range1944404
display id: range1944404
version: 1
direction: direction/forward
top level: U049O11+D0
start: 3
end: 8
orientation: inline

BBa_E1010
type: SequenceAnnotation
persistent identity: annotation1944416
display id: annotation1944416
version: 1
top level: U049O11+D0
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_I732405/annotation1944416/range1944416/1

https://synbiohub.org/public/igem/BBa_I732405/annotation1944416/range1944416/1
type: Range
persistent identity: range1944416
display id: range1944416
version: 1
direction: direction/reverse
top level: U049O11+D0
start: 306
end: 986
orientation: inline

BBa_I732006
type: SequenceAnnotation
persistent identity: annotation1944412
display id: annotation1944412
version: 1
top level: U049O11+D0
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_I732405/annotation1944412/range1944412/1

https://synbiohub.org/public/igem/BBa_I732405/annotation1944412/range1944412/1
type: Range
persistent identity: range1944412
display id: range1944412
version: 1
direction: direction/reverse
top level: U049O11+D0
start: 46
end: 279
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1944418
display id: annotation1944418
version: 1
top level: U049O11+D0
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_I732405/annotation1944418/range1944418/1

https://synbiohub.org/public/igem/BBa_I732405/annotation1944418/range1944418/1
type: Range
persistent identity: range1944418
display id: range1944418
version: 1
direction: direction/forward
top level: U049O11+D0
start: 1020
end: 1099
orientation: inline

START
type: SequenceAnnotation
persistent identity: annotation1944409
display id: annotation1944409
version: 1
top level: U049O11+D0
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_I732405/annotation1944409/range1944409/1

https://synbiohub.org/public/igem/BBa_I732405/annotation1944409/range1944409/1
type: Range
persistent identity: range1944409
display id: range1944409
version: 1
direction: direction/forward
top level: U049O11+D0
start: 46
end: 48
orientation: inline

lux pR-5
type: ComponentDefinition
persistent identity: BBa K658007
display id: BBa_K658007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: position 5 mutated promoter lux pR-5 (luxR & HSL regulated)
creator: Yidan Hu
created: 2011-09-30T11:00:00Z
modified: 2015-05-08T01:13:02Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _835_
m user id: 0
owner id: 10207
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: The position 5 mutated promoter lux pR-5 is a product of site-directed mutagenesis of promoter lux pR (BBa_R0062). The promoter lux pR is based on the Vibrio fischeri quorum sensing gene promoters. The position 5 mutated promoter lux pR-5 gives a higher expression of downstream genes in an autoinduction system. In another word, the lux pR-5 has a higher efficiency than the lux pR. Similar to promoter lux pR, the expression is up-regulated by the action of LuxR protein combined with the signal molecule HSL.
mutable notes: By PCR method the G residue of position 5 was changed to a C based on the sequence of the promoter lux pR(BBa_R0062).
mutable provenance: The promoter lux pR (BBa_R0062).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: start
sequence annotation: -10
sequence annotation: LuxR/HSL
sequence annotation: K658007
sequence annotation: -35
sequence: https://synbiohub.org/public/igem/BBa_K658007_sequence/1

start
type: SequenceAnnotation
persistent identity: annotation2148167
display id: annotation2148167
version: 1
top level: lux pR-5
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K658007/annotation2148167/range2148167/1

https://synbiohub.org/public/igem/BBa_K658007/annotation2148167/range2148167/1
type: Range
persistent identity: range2148167
display id: range2148167
version: 1
direction: direction/forward
top level: lux pR-5
start: 53
end: 53
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2148171
display id: annotation2148171
version: 1
top level: lux pR-5
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K658007/annotation2148171/range2148171/1

https://synbiohub.org/public/igem/BBa_K658007/annotation2148171/range2148171/1
type: Range
persistent identity: range2148171
display id: range2148171
version: 1
direction: direction/forward
top level: lux pR-5
start: 42
end: 47
orientation: inline

LuxR/HSL
type: SequenceAnnotation
persistent identity: annotation2148169
display id: annotation2148169
version: 1
top level: lux pR-5
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K658007/annotation2148169/range2148169/1

https://synbiohub.org/public/igem/BBa_K658007/annotation2148169/range2148169/1
type: Range
persistent identity: range2148169
display id: range2148169
version: 1
direction: direction/forward
top level: lux pR-5
start: 1
end: 20
orientation: inline

K658007
type: SequenceAnnotation
persistent identity: annotation2148168
display id: annotation2148168
version: 1
top level: lux pR-5
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K658007/annotation2148168/range2148168/1

https://synbiohub.org/public/igem/BBa_K658007/annotation2148168/range2148168/1
type: Range
persistent identity: range2148168
display id: range2148168
version: 1
direction: direction/forward
top level: lux pR-5
start: 1
end: 55
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2148170
display id: annotation2148170
version: 1
top level: lux pR-5
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K658007/annotation2148170/range2148170/1

https://synbiohub.org/public/igem/BBa_K658007/annotation2148170/range2148170/1
type: Range
persistent identity: range2148170
display id: range2148170
version: 1
direction: direction/forward
top level: lux pR-5
start: 20
end: 25
orientation: inline

BBa_I742146
type: ComponentDefinition
persistent identity: BBa I742146
display id: BBa_I742146
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: sam8 native rbs
creator: Chris French
created: 2007-10-23T11:00:00Z
modified: 2015-08-31T04:08:03Z
discontinued: false
dominant: false
experience: experience/None
group u list: _123_
m user id: 0
owner id: 837
owning group id: 163
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Native ribosome binding site of sam8 (tyrosine ammonia lyase) from the bacterium Saccharothrix espanaensis DSM 44229.
mutable notes: Virtual part to be added to coding sequence I742142.
mutable provenance: Saccharothrix espanaensis DSM 44229.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_I742146_sequence/1

BBa_M31370
type: ComponentDefinition
persistent identity: BBa M31370
display id: BBa_M31370
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: tacI Promoter
creator: Michael Oh
created: 2007-02-28T12:00:00Z
modified: 2015-05-08T01:14:00Z
discontinued: false
dominant: false
group u list: _102_
m user id: 0
owner id: 1395
owning group id: 102
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Allows for regulation of following gene with laqIq and IPTG...laqIq in same plasmid represses the promoter and IPTG proportionally depresses the promoter (Boer, et al., 1983).
mutable notes: Took sequence from Boer, 1983 paper.
mutable provenance: Derived from trp and lac promoter sequences.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_M31370_sequence/1

BBa_I766200
type: ComponentDefinition
persistent identity: BBa I766200
display id: BBa_I766200
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pSte2
creator: Nili Sommovilla
created: 2007-10-21T11:00:00Z
modified: 2015-08-31T04:08:09Z
discontinued: false
dominant: false
group u list: _155_
m user id: 0
owner id: 1931
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: endogenous promoter of Ste2
mutable notes: none.
mutable provenance: from yeast genome
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I766200_sequence/1

NOR2
type: ComponentDefinition
persistent identity: BBa I732352
display id: BBa_I732352
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: NOR Candidate (U035O44D001O22)
creator: Zhan Jian
created: 2007-10-02T11:00:00Z
modified: 2015-08-31T04:07:58Z
discontinued: false
dominant: false
experience: experience/Issues
group u list: _156_
m user id: 0
owner id: 1592
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: U97NUL D001O61
mutable notes: .
mutable provenance: .
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732352_sequence/1

U037O11+D0
type: ComponentDefinition
persistent identity: BBa I732443
display id: BBa_I732443
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Family Member (U037O11+D002O22)
creator: Zhan Jian
created: 2007-10-13T11:00:00Z
modified: 2015-08-31T04:07:58Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732443_sequence/1

BBa_K1486036
type: ComponentDefinition
persistent identity: BBa K1486036
display id: BBa_K1486036
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: rLucC + ADH1 terminator + CaUra3 cassette
creator: EPFL iGem team 2014
created: 2014-09-26T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: To edit
mutable notes: To edit
mutable provenance: To edit
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000324
role: partType/Tag
component: BBa_K1486020
component: BBa_K1486031
component: BBa_K1486037
component: BBa_K1486025
sequence annotation: BBa_K1486031
sequence annotation: BBa_K1486025
sequence annotation: BBa_K1486037
sequence annotation: BBa_K1486020
sequence: https://synbiohub.org/public/igem/BBa_K1486036_sequence/1

BBa_K1486020
type: Component
persistent identity: component2418338
display id: component2418338
version: 1
top level: BBa_K1486036
access: public
definition: BBa_K1486020

BBa_K1486031
type: Component
persistent identity: component2418343
display id: component2418343
version: 1
top level: BBa_K1486036
access: public
definition: BBa_K1486031

BBa_K1486037
type: Component
persistent identity: component2418337
display id: component2418337
version: 1
top level: BBa_K1486036
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486037/1

BBa_K1486025
type: Component
persistent identity: component2418339
display id: component2418339
version: 1
top level: BBa_K1486036
access: public
definition: BBa_K1486025

BBa_K1486031
type: SequenceAnnotation
persistent identity: annotation2418343
display id: annotation2418343
version: 1
top level: BBa_K1486036
location: https://synbiohub.org/public/igem/BBa_K1486036/annotation2418343/range2418343/1
component: BBa_K1486031

https://synbiohub.org/public/igem/BBa_K1486036/annotation2418343/range2418343/1
type: Range
persistent identity: range2418343
display id: range2418343
version: 1
direction: direction/reverse
top level: BBa_K1486036
start: 834
end: 2193
orientation: inline

BBa_K1486025
type: SequenceAnnotation
persistent identity: annotation2418339
display id: annotation2418339
version: 1
top level: BBa_K1486036
location: https://synbiohub.org/public/igem/BBa_K1486036/annotation2418339/range2418339/1
component: BBa_K1486025

https://synbiohub.org/public/igem/BBa_K1486036/annotation2418339/range2418339/1
type: Range
persistent identity: range2418339
display id: range2418339
version: 1
direction: direction/reverse
top level: BBa_K1486036
start: 646
end: 833
orientation: inline

BBa_K1486037
type: SequenceAnnotation
persistent identity: annotation2418337
display id: annotation2418337
version: 1
top level: BBa_K1486036
location: https://synbiohub.org/public/igem/BBa_K1486036/annotation2418337/range2418337/1
component: BBa_K1486037

https://synbiohub.org/public/igem/BBa_K1486036/annotation2418337/range2418337/1
type: Range
persistent identity: range2418337
display id: range2418337
version: 1
direction: direction/reverse
top level: BBa_K1486036
start: 1
end: 39
orientation: inline

BBa_K1486020
type: SequenceAnnotation
persistent identity: annotation2418338
display id: annotation2418338
version: 1
top level: BBa_K1486036
location: https://synbiohub.org/public/igem/BBa_K1486036/annotation2418338/range2418338/1
component: BBa_K1486020

https://synbiohub.org/public/igem/BBa_K1486036/annotation2418338/range2418338/1
type: Range
persistent identity: range2418338
display id: range2418338
version: 1
direction: direction/reverse
top level: BBa_K1486036
start: 40
end: 645
orientation: inline

BBa_Z0262
type: ComponentDefinition
persistent identity: BBa Z0262
display id: BBa_Z0262
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Medium strength T7.2 RBS
creator: Sriram Kosuri
created: 2006-05-15T11:00:00Z
modified: 2015-05-08T01:14:55Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_10_
m user id: 0
owner id: 64
owning group id: 10
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Sequence comes from the natural B0030. Using B0030 as a medium strength RBS is from initial results from experiments characterizing all natural T7 RBSs.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_Z0262_sequence/1

BBa_K1165003
type: ComponentDefinition
persistent identity: BBa K1165003
display id: BBa_K1165003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PenI-cleaving Ribozyme 2 of XOR Gate 1
creator: iGEM Concordia 2013
created: 2013-09-26T11:00:00Z
modified: 2015-05-08T01:09:33Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1477_
m user id: 0
owner id: 18237
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: -
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000834
role: partType/RNA
sequence annotation: TAA Bump
sequence annotation: Synthetic Extended Arms
sequence annotation: Target Binding Arms
sequence annotation: Target-Binding Arms
sequence annotation: Catalytic Core
sequence: https://synbiohub.org/public/igem/BBa_K1165003_sequence/1

TAA Bump
type: SequenceAnnotation
persistent identity: annotation2365211
display id: annotation2365211
version: 1
top level: BBa_K1165003
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1165003/annotation2365211/range2365211/1

https://synbiohub.org/public/igem/BBa_K1165003/annotation2365211/range2365211/1
type: Range
persistent identity: range2365211
display id: range2365211
version: 1
direction: direction/forward
top level: BBa_K1165003
start: 36
end: 38
orientation: inline

Synthetic Extended Arms
type: SequenceAnnotation
persistent identity: annotation2365209
display id: annotation2365209
version: 1
top level: BBa_K1165003
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1165003/annotation2365209/range2365209/1

https://synbiohub.org/public/igem/BBa_K1165003/annotation2365209/range2365209/1
type: Range
persistent identity: range2365209
display id: range2365209
version: 1
direction: direction/forward
top level: BBa_K1165003
start: 1
end: 30
orientation: inline

Target Binding Arms
type: SequenceAnnotation
persistent identity: annotation2365214
display id: annotation2365214
version: 1
top level: BBa_K1165003
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1165003/annotation2365214/range2365214/1

https://synbiohub.org/public/igem/BBa_K1165003/annotation2365214/range2365214/1
type: Range
persistent identity: range2365214
display id: range2365214
version: 1
direction: direction/forward
top level: BBa_K1165003
start: 70
end: 81
orientation: inline

Target-Binding Arms
type: SequenceAnnotation
persistent identity: annotation2365210
display id: annotation2365210
version: 1
top level: BBa_K1165003
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1165003/annotation2365210/range2365210/1

https://synbiohub.org/public/igem/BBa_K1165003/annotation2365210/range2365210/1
type: Range
persistent identity: range2365210
display id: range2365210
version: 1
direction: direction/forward
top level: BBa_K1165003
start: 32
end: 35
orientation: inline

Target-Binding Arms
type: SequenceAnnotation
persistent identity: annotation2365212
display id: annotation2365212
version: 1
top level: BBa_K1165003
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1165003/annotation2365212/range2365212/1

https://synbiohub.org/public/igem/BBa_K1165003/annotation2365212/range2365212/1
type: Range
persistent identity: range2365212
display id: range2365212
version: 1
direction: direction/forward
top level: BBa_K1165003
start: 39
end: 43
orientation: inline

Synthetic Extended Arms
type: SequenceAnnotation
persistent identity: annotation2365215
display id: annotation2365215
version: 1
top level: BBa_K1165003
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1165003/annotation2365215/range2365215/1

https://synbiohub.org/public/igem/BBa_K1165003/annotation2365215/range2365215/1
type: Range
persistent identity: range2365215
display id: range2365215
version: 1
direction: direction/forward
top level: BBa_K1165003
start: 82
end: 111
orientation: inline

Catalytic Core
type: SequenceAnnotation
persistent identity: annotation2365213
display id: annotation2365213
version: 1
top level: BBa_K1165003
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1165003/annotation2365213/range2365213/1

https://synbiohub.org/public/igem/BBa_K1165003/annotation2365213/range2365213/1
type: Range
persistent identity: range2365213
display id: range2365213
version: 1
direction: direction/forward
top level: BBa_K1165003
start: 44
end: 69
orientation: inline

BBa_J54016
type: ComponentDefinition
persistent identity: BBa J54016
display id: BBa_J54016
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: promoter_lacq
creator: Yusaku Nakashima
created: 2006-10-14T11:00:00Z
modified: 2015-08-31T03:48:11Z
discontinued: false
dominant: false
group u list: _76_
m user id: 0
owner id: 762
owning group id: 76
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: lacq promoter
mutable notes: As Promoter
mutable provenance: DNA synthesis
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: BamHI
sequence annotation: promoter_lacq
sequence: https://synbiohub.org/public/igem/BBa_J54016_sequence/1

BamHI
type: SequenceAnnotation
persistent identity: annotation1903168
display id: annotation1903168
version: 1
top level: BBa_J54016
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_J54016/annotation1903168/range1903168/1

https://synbiohub.org/public/igem/BBa_J54016/annotation1903168/range1903168/1
type: Range
persistent identity: range1903168
display id: range1903168
version: 1
direction: direction/forward
top level: BBa_J54016
start: 12
end: 17
orientation: inline

promoter_lacq
type: SequenceAnnotation
persistent identity: annotation1903169
display id: annotation1903169
version: 1
top level: BBa_J54016
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_J54016/annotation1903169/range1903169/1

https://synbiohub.org/public/igem/BBa_J54016/annotation1903169/range1903169/1
type: Range
persistent identity: range1903169
display id: range1903169
version: 1
direction: direction/forward
top level: BBa_J54016
start: 18
end: 49
orientation: inline

BBa_K227006
type: ComponentDefinition
persistent identity: BBa K227006
display id: BBa_K227006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: puc BA coding region of R. sphaeroides
creator: WashU iGEM 2009
created: 2009-10-11T11:00:00Z
modified: 2015-05-08T01:11:32Z
discontinued: false
dominant: false
experience: experience/None
group u list: _326_
m user id: 0
owner id: 4645
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: puc BA is the complete LHII transmembrane protein coding region found naturally in Rhodobacter Sphaeroides. LHII complexes form the light-harvesting antennae the funnel energy down to LHI and the reaction center. They are naturally found in the ratio of 3-6.7 LHII per LHI. Data suggests the precence of puc C,D, and E regions also contribute to the formation of a functional protein.
mutable notes: puc A and puc B together create the antennae complex necessary to harvest varying intensities of light. A common problem in bioreactors is the uneven distribution of light between cells nearest the light source and cells farther away from the light source. One way of combating this problem is modify the antenna size of the organism. By truncating the light-harvesting antenna, cells at the exterior of the bioreactor waste less light through Non-Photochemical Quenching, thus allowing photons to penetrate deeper and reach cells in the interior of the bioreactor. This leads to an increase in photosynthetic efficiency and productivity for the entire culture.
mutable provenance: PCR applified from plasmid pRKCBC3 provided by Dr. Neil Hunter.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: pucA
sequence annotation: pucB
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_K227006_sequence/1

pucA
type: SequenceAnnotation
persistent identity: annotation2057321
display id: annotation2057321
version: 1
top level: BBa_K227006
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K227006/annotation2057321/range2057321/1

https://synbiohub.org/public/igem/BBa_K227006/annotation2057321/range2057321/1
type: Range
persistent identity: range2057321
display id: range2057321
version: 1
direction: direction/forward
top level: BBa_K227006
start: 172
end: 336
orientation: inline

pucB
type: SequenceAnnotation
persistent identity: annotation2057319
display id: annotation2057319
version: 1
top level: BBa_K227006
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K227006/annotation2057319/range2057319/1

https://synbiohub.org/public/igem/BBa_K227006/annotation2057319/range2057319/1
type: Range
persistent identity: range2057319
display id: range2057319
version: 1
direction: direction/forward
top level: BBa_K227006
start: 1
end: 156
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2057320
display id: annotation2057320
version: 1
top level: BBa_K227006
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K227006/annotation2057320/range2057320/1

https://synbiohub.org/public/igem/BBa_K227006/annotation2057320/range2057320/1
type: Range
persistent identity: range2057320
display id: range2057320
version: 1
direction: direction/forward
top level: BBa_K227006
start: 159
end: 163
orientation: inline

mRFP1-NLS
type: ComponentDefinition
persistent identity: BBa K678034
display id: BBa_K678034
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: mRFP1-NLS, targeted to the nucleus
creator: DTU-Denmark-2
created: 2011-09-09T11:00:00Z
modified: 2015-05-08T01:13:04Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _882_
m user id: 0
owner id: 8341
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: mRFP
mutable notes: This part was designed for the Plug'n'Play with DNA assembly standard (RFC 80), therefore this part contains tails at the 5' and 3' ends as shown on the figure below. Please notice that mRFP1-NLS is not the same as BBa_K678030, BBa_K678031, or BBa_K678032.
mutable provenance: From p71-mRFP1 of the Center for Microbial Biotechnology plasmid collection at the Technical University of Denmark. The NLS sequence was synthesized.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: stop
sequence annotation: Linker 2
sequence annotation: start
sequence annotation: RFP
sequence annotation: NLS
sequence: https://synbiohub.org/public/igem/BBa_K678034_sequence/1

stop
type: SequenceAnnotation
persistent identity: annotation2126905
display id: annotation2126905
version: 1
top level: mRFP1-NLS
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K678034/annotation2126905/range2126905/1

https://synbiohub.org/public/igem/BBa_K678034/annotation2126905/range2126905/1
type: Range
persistent identity: range2126905
display id: range2126905
version: 1
direction: direction/forward
top level: mRFP1-NLS
start: 705
end: 707
orientation: inline

Linker 2
type: SequenceAnnotation
persistent identity: annotation2126904
display id: annotation2126904
version: 1
top level: mRFP1-NLS
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K678034/annotation2126904/range2126904/1

https://synbiohub.org/public/igem/BBa_K678034/annotation2126904/range2126904/1
type: Range
persistent identity: range2126904
display id: range2126904
version: 1
direction: direction/forward
top level: mRFP1-NLS
start: 1
end: 8
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2126906
display id: annotation2126906
version: 1
top level: mRFP1-NLS
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K678034/annotation2126906/range2126906/1

https://synbiohub.org/public/igem/BBa_K678034/annotation2126906/range2126906/1
type: Range
persistent identity: range2126906
display id: range2126906
version: 1
direction: direction/forward
top level: mRFP1-NLS
start: 9
end: 11
orientation: inline

RFP
type: SequenceAnnotation
persistent identity: annotation2127400
display id: annotation2127400
version: 1
top level: mRFP1-NLS
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K678034/annotation2127400/range2127400/1

https://synbiohub.org/public/igem/BBa_K678034/annotation2127400/range2127400/1
type: Range
persistent identity: range2127400
display id: range2127400
version: 1
direction: direction/forward
top level: mRFP1-NLS
start: 9
end: 683
orientation: inline

NLS
type: SequenceAnnotation
persistent identity: annotation2126903
display id: annotation2126903
version: 1
top level: mRFP1-NLS
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K678034/annotation2126903/range2126903/1

https://synbiohub.org/public/igem/BBa_K678034/annotation2126903/range2126903/1
type: Range
persistent identity: range2126903
display id: range2126903
version: 1
direction: direction/forward
top level: mRFP1-NLS
start: 684
end: 704
orientation: inline

BBa_J64997
type: ComponentDefinition
persistent identity: BBa J64997
display id: BBa_J64997
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: T7 consensus -10 and rest
creator: Sai Duriseti
created: 2007-03-25T11:00:00Z
modified: 2015-05-08T01:08:18Z
discontinued: false
dominant: false
experience: experience/None
group u list: _98_
m user id: 0
owner id: 1428
owning group id: 98
sample status: Not in stock
status: status/Unavailable
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: T7-10
sequence: https://synbiohub.org/public/igem/BBa_J64997_sequence/1

T7-10
type: SequenceAnnotation
persistent identity: annotation1921746
display id: annotation1921746
version: 1
top level: BBa_J64997
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_J64997/annotation1921746/range1921746/1

https://synbiohub.org/public/igem/BBa_J64997/annotation1921746/range1921746/1
type: Range
persistent identity: range1921746
display id: range1921746
version: 1
direction: direction/forward
top level: BBa_J64997
start: 1
end: 19
orientation: inline

BBa_K091111
type: ComponentDefinition
persistent identity: BBa K091111
display id: BBa_K091111
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LacIQ promoter
creator: Robert Cool
created: 2008-06-19T11:00:00Z
modified: 2015-05-08T01:08:37Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _191_
m user id: 0
owner id: 3080
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This is a modified version of the LacIQ promoter tha should bind LacIQ tighter than the original.
mutable notes: no special considerations
mutable provenance: This is a modified version of the LacIQ promoter
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -10
sequence annotation: -35
sequence: https://synbiohub.org/public/igem/BBa_K091111_sequence/1

-10
type: SequenceAnnotation
persistent identity: annotation1964080
display id: annotation1964080
version: 1
top level: BBa_K091111
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K091111/annotation1964080/range1964080/1

https://synbiohub.org/public/igem/BBa_K091111/annotation1964080/range1964080/1
type: Range
persistent identity: range1964080
display id: range1964080
version: 1
direction: direction/forward
top level: BBa_K091111
start: 44
end: 49
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1964079
display id: annotation1964079
version: 1
top level: BBa_K091111
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K091111/annotation1964079/range1964079/1

https://synbiohub.org/public/igem/BBa_K091111/annotation1964079/range1964079/1
type: Range
persistent identity: range1964079
display id: range1964079
version: 1
direction: direction/forward
top level: BBa_K091111
start: 17
end: 22
orientation: inline

BBa_K863201
type: ComponentDefinition
persistent identity: BBa K863201
display id: BBa_K863201
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: 3' UTR site of alcohol oxidase 1 gene (aox1)
creator: Julia Schirmacher
created: 2012-09-22T11:00:00Z
modified: 2015-05-08T01:13:36Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1123_
m user id: 0
owner id: 13076
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: 3' UTR site of alcohol oxidase 1 gene (AOX1) from K. pastoris used as a recombination site.
mutable notes: primer sequences are coming soon
mutable provenance: The DNA sequence from plasmid pPIC9K of Invitrogen (http://products.invitrogen.com/ivgn/product/V17520?ICID==%3Dsearch-product) was used for primer design. But the genomic DNA of Pichia pastoris wildtype X33 was used for amplification via PCR.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_K863201_sequence/1

BBa_B0076
type: ComponentDefinition
persistent identity: BBa B0076
display id: BBa_B0076
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Specialized RBS
creator: Bartholomew Canton
created: 2006-05-01T11:00:00Z
modified: 2015-08-31T04:07:21Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_6_
m user id: 0
owner id: 135
owning group id: 6
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_B0076_sequence/1

BBa_K082001
type: ComponentDefinition
persistent identity: BBa K082001
display id: BBa_K082001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS medium
creator: Yanting Xue , Binjie Xu
created: 2008-10-27T12:00:00Z
modified: 2015-05-08T01:08:35Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _228_
m user id: 0
owner id: 1560
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Weak1 RBS based on Ron Weiss thesis. Strength is considered relative to BBa_B0030, BBa_B0031, BBa_B0033.
mutable notes: the
mutable provenance: the
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: BBa_K082001
sequence: https://synbiohub.org/public/igem/BBa_K082001_sequence/1

BBa_K082001
type: SequenceAnnotation
persistent identity: annotation1993438
display id: annotation1993438
version: 1
top level: BBa_K082001
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K082001/annotation1993438/range1993438/1

https://synbiohub.org/public/igem/BBa_K082001/annotation1993438/range1993438/1
type: Range
persistent identity: range1993438
display id: range1993438
version: 1
direction: direction/reverse
top level: BBa_K082001
start: 1
end: 15
orientation: inline

BBa_J23106
type: ComponentDefinition
persistent identity: BBa J23106
display id: BBa_J23106
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: constitutive promoter family member
creator: John Anderson
created: 2006-08-13T11:00:00Z
modified: 2015-08-31T04:08:40Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: Later
mutable notes: N/A
mutable provenance: Isolated from library of promoters
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J23106_sequence/1

BBa_K1486045
type: ComponentDefinition
persistent identity: BBa K1486045
display id: BBa_K1486045
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: IFP[2] Mutated
creator: EPFL iGem team 2014
created: 2014-10-06T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1866_
m user id: 0
owner id: 21033
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is a test
mutable notes: Test
mutable provenance: Test
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: IFP2
sequence: https://synbiohub.org/public/igem/BBa_K1486045_sequence/1

IFP2
type: SequenceAnnotation
persistent identity: annotation2420793
display id: annotation2420793
version: 1
top level: BBa_K1486045
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1486045/annotation2420793/range2420793/1

https://synbiohub.org/public/igem/BBa_K1486045/annotation2420793/range2420793/1
type: Range
persistent identity: range2420793
display id: range2420793
version: 1
direction: direction/forward
top level: BBa_K1486045
start: 1
end: 582
orientation: inline

NAND01
type: ComponentDefinition
persistent identity: BBa I732303
display id: BBa_I732303
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: NAND Candidate (U073O22D001O46)
creator: Ma Rui
created: 2007-10-02T11:00:00Z
modified: 2015-08-31T04:07:57Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _156_
m user id: 0
owner id: 1592
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: U97NUL D001O11
mutable notes: .
mutable provenance: .
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732303_sequence/1

BBa_J61129
type: ComponentDefinition
persistent identity: BBa J61129
display id: BBa_J61129
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Ribosome Binding Site Family Member
creator: John Anderson
created: 2007-04-24T11:00:00Z
modified: 2015-08-31T01:59:49Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: false
mutable description: {{JCA_Arkin_RBSFamily}}
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J61129_sequence/1

BBa_J61113
type: ComponentDefinition
persistent identity: BBa J61113
display id: BBa_J61113
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Ribosome Binding Site Family Member
creator: John Anderson
created: 2007-04-23T11:00:00Z
modified: 2015-08-31T02:03:00Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: false
mutable description: {{JCA_Arkin_RBSFamily}}
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J61113_sequence/1

BBa_J22106
type: ComponentDefinition
persistent identity: BBa J22106
display id: BBa_J22106
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: rec A (SOS) Promoter
creator: Krithiga Sankaran
created: 2006-09-04T11:00:00Z
modified: 2015-08-31T04:08:38Z
discontinued: false
dominant: true
group u list: _70_
m user id: 0
owner id: 1038
owning group id: 70
sample status: In stock
status: status/Available
bookmark: false
mutable description: Rec A (SOS) promoter.
mutable notes: NA
mutable provenance: The recA(SOS)promoter gene from the chromosomal DNA isolated from MG1655 has been PCR amplified and inserted into plasmid pSB1A2(part number BBa_B0015).
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J22106_sequence/1

BBa_K124002
type: ComponentDefinition
persistent identity: BBa K124002
display id: BBa_K124002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Yeast GPD (TDH3) Promoter
creator: John Szymanski
created: 2008-06-30T11:00:00Z
modified: 2015-05-08T01:09:44Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _221_
m user id: 0
owner id: 2512
owning group id: 58
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Regulatory region spanning 680 bp upstream of the start codon of the GPD1 gene in yeast. Very strong constitutive promoter.
mutable notes: none
mutable provenance: Mumberg, Muller, and Funk. p413 pGPD http://dx.doi.org/10.1016/0378-1119(95)00037-7
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K124002_sequence/1

BBa_K292003
type: ComponentDefinition
persistent identity: BBa K292003
display id: BBa_K292003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Kanamycin resistance cassette and a double terminator
creator: David Charoy
created: 2009-10-18T11:00:00Z
modified: 2015-05-08T01:11:49Z
discontinued: false
dominant: false
group u list: _394_
m user id: 0
owner id: 5212
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part contains a kanamycin resistance cassette and a double terminator (BBa_P1003 + BBa_B0014). This allows the kanamycin cassette to be functional. The cassette can be used to design a recombinant vector by gene insertion in a non lytic bacteriophage as the Lambda phage. In fact it allows us to know if a bacteriophage is modified or not and if the inserted gene is expressed or not. When the virus is modified the kanamycin cassette induces a kanamycin resistance, so the bacteria become resistance, and when the virus is not modified bacteria are killed by kanamycin. This part is completed by B0014 + P1003 + B0014 to finish the transcription before the resistance cassette.
mutable notes: When we design the bio-brick it was impossible to obtain a functional bio-brick by using the plasmid SB1AK3. But it's worked with a pSB1A1.
mutable provenance: Part:BBa_P1003, Designed by Reshma Shetty Group: Knight Lab, MIT (2006-01-23) Part:BBa_B0014, Designed by Reshma Shetty Group: Registry (2003-07-16)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_P1003
component: BBa_B0011
component: BBa_B0012
sequence annotation: BBa_P1003
sequence annotation: BBa_B0012
sequence annotation: BBa_B0011
sequence: https://synbiohub.org/public/igem/BBa_K292003_sequence/1

BBa_P1003
type: Component
persistent identity: component2054557
display id: component2054557
version: 1
top level: BBa_K292003
access: public
definition: kanR

BBa_B0011
type: Component
persistent identity: component2054562
display id: component2054562
version: 1
top level: BBa_K292003
access: public
definition: https://synbiohub.org/public/igem/BBa_B0011/1

BBa_B0012
type: Component
persistent identity: component2054558
display id: component2054558
version: 1
top level: BBa_K292003
access: public
definition: BBa_B0012

BBa_P1003
type: SequenceAnnotation
persistent identity: annotation2054557
display id: annotation2054557
version: 1
top level: BBa_K292003
location: https://synbiohub.org/public/igem/BBa_K292003/annotation2054557/range2054557/1
component: BBa_P1003

https://synbiohub.org/public/igem/BBa_K292003/annotation2054557/range2054557/1
type: Range
persistent identity: range2054557
display id: range2054557
version: 1
direction: direction/reverse
top level: BBa_K292003
start: 1
end: 967
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2054558
display id: annotation2054558
version: 1
top level: BBa_K292003
location: https://synbiohub.org/public/igem/BBa_K292003/annotation2054558/range2054558/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K292003/annotation2054558/range2054558/1
type: Range
persistent identity: range2054558
display id: range2054558
version: 1
direction: direction/forward
top level: BBa_K292003
start: 976
end: 1016
orientation: inline

BBa_B0011
type: SequenceAnnotation
persistent identity: annotation2054562
display id: annotation2054562
version: 1
top level: BBa_K292003
location: https://synbiohub.org/public/igem/BBa_K292003/annotation2054562/range2054562/1
component: BBa_B0011

https://synbiohub.org/public/igem/BBa_K292003/annotation2054562/range2054562/1
type: Range
persistent identity: range2054562
display id: range2054562
version: 1
direction: direction/forward
top level: BBa_K292003
start: 1025
end: 1070
orientation: inline

BBa_K145150
type: ComponentDefinition
persistent identity: BBa K145150
display id: BBa_K145150
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Hybrid promoter: HSL-LuxR activated, P22 C2 repressed
creator: Jonas Demeulemeester
created: 2008-08-05T11:00:00Z
modified: 2015-05-08T01:10:29Z
discontinued: false
dominant: false
experience: experience/None
group u list: _257_
m user id: 0
owner id: 2970
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Hybrid promoter consisting of the Lux box which overlaps partly with -35 box. Binding sites for P22 C2 OR2 and OR1 are located between the -35 and -10 boxes and downstream of them respectively.
mutable notes: -35 box is partly composite. -10 is taken from the P22 phage PR regulatory region
mutable provenance: Synthetic
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: OR2
sequence annotation: OR1
sequence annotation: -10
sequence annotation: Lux-box
sequence annotation: -35
sequence: https://synbiohub.org/public/igem/BBa_K145150_sequence/1

OR2
type: SequenceAnnotation
persistent identity: annotation1971584
display id: annotation1971584
version: 1
top level: BBa_K145150
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K145150/annotation1971584/range1971584/1

https://synbiohub.org/public/igem/BBa_K145150/annotation1971584/range1971584/1
type: Range
persistent identity: range1971584
display id: range1971584
version: 1
direction: direction/forward
top level: BBa_K145150
start: 23
end: 40
orientation: inline

OR1
type: SequenceAnnotation
persistent identity: annotation1971586
display id: annotation1971586
version: 1
top level: BBa_K145150
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K145150/annotation1971586/range1971586/1

https://synbiohub.org/public/igem/BBa_K145150/annotation1971586/range1971586/1
type: Range
persistent identity: range1971586
display id: range1971586
version: 1
direction: direction/forward
top level: BBa_K145150
start: 46
end: 63
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1971585
display id: annotation1971585
version: 1
top level: BBa_K145150
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K145150/annotation1971585/range1971585/1

https://synbiohub.org/public/igem/BBa_K145150/annotation1971585/range1971585/1
type: Range
persistent identity: range1971585
display id: range1971585
version: 1
direction: direction/forward
top level: BBa_K145150
start: 42
end: 47
orientation: inline

Lux-box
type: SequenceAnnotation
persistent identity: annotation1971582
display id: annotation1971582
version: 1
top level: BBa_K145150
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K145150/annotation1971582/range1971582/1

https://synbiohub.org/public/igem/BBa_K145150/annotation1971582/range1971582/1
type: Range
persistent identity: range1971582
display id: range1971582
version: 1
direction: direction/forward
top level: BBa_K145150
start: 1
end: 20
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1971583
display id: annotation1971583
version: 1
top level: BBa_K145150
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K145150/annotation1971583/range1971583/1

https://synbiohub.org/public/igem/BBa_K145150/annotation1971583/range1971583/1
type: Range
persistent identity: range1971583
display id: range1971583
version: 1
direction: direction/forward
top level: BBa_K145150
start: 20
end: 25
orientation: inline

mRFP1
type: ComponentDefinition
persistent identity: BBa K678031
display id: BBa_K678031
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: mRFP1-GOI
creator: DTU-Denmark-2
created: 2011-09-08T11:00:00Z
modified: 2015-05-08T01:13:04Z
discontinued: false
dominant: false
experience: experience/None
group u list: _882_
m user id: 0
owner id: 8341
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Monomeric red fluorescent protein (mRFP1) is a derivative of the red fluorescent protein cloned from Discosoma coral (DsRed) (1). The successful use of mRFP1 in Aspergillus nidulans has previously been described (2). This part does not contain a stop codon. {| border="1" !colspan="8"|Spectroscopic data (1) |- |Excitation maximum |Emission maximum |Extinction coefficient per chain |Fluorescence quantum yield |pKa |t0.5 for maturation at 37??C |colspan="2"|Rate of photobleach relative to EGFP |- |584 nm |607 nm |44,000 M-1cm-1 |0.25 |4.5 |<1h |fast 7.2 |slow 0.16 |}
mutable notes: This part was designed for the Plug'n'Play with DNA assembly standard (RFC 80), therefore this part contains tails at the 5' and 3' ends as shown on the figure below. Please notice that mRFP1-module is not the same as BBa_K678030 or BBa_K678032.
mutable provenance: From p71-mRFP1 of the Center for Microbial Biotechnology plasmid collection at the Technical University of Denmark
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: Linker 2
sequence annotation: RFP
sequence: https://synbiohub.org/public/igem/BBa_K678031_sequence/1

Linker 2
type: SequenceAnnotation
persistent identity: annotation2127390
display id: annotation2127390
version: 1
top level: mRFP1
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K678031/annotation2127390/range2127390/1

https://synbiohub.org/public/igem/BBa_K678031/annotation2127390/range2127390/1
type: Range
persistent identity: range2127390
display id: range2127390
version: 1
direction: direction/forward
top level: mRFP1
start: 1
end: 8
orientation: inline

RFP
type: SequenceAnnotation
persistent identity: annotation2127391
display id: annotation2127391
version: 1
top level: mRFP1
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K678031/annotation2127391/range2127391/1

https://synbiohub.org/public/igem/BBa_K678031/annotation2127391/range2127391/1
type: Range
persistent identity: range2127391
display id: range2127391
version: 1
direction: direction/forward
top level: mRFP1
start: 9
end: 683
orientation: inline

BBa_K086018
type: ComponentDefinition
persistent identity: BBa K086018
display id: BBa_K086018
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: modified Lutz-Bujard LacO promoter,with alternative sigma factor σ24
creator: Gairik Sachdeva
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:08:36Z
discontinued: false
dominant: false
group u list: _197_
m user id: 0
owner id: 2578
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: modified Lutz-Bujard LacO promoter,with alternative sigma factor σ24
mutable notes: Cleaned
mutable provenance: LacO and Sigma 24 binding conserved sequences
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: P24_1
sequence: https://synbiohub.org/public/igem/BBa_K086018_sequence/1

P24_1
type: SequenceAnnotation
persistent identity: annotation1995752
display id: annotation1995752
version: 1
top level: BBa_K086018
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K086018/annotation1995752/range1995752/1

https://synbiohub.org/public/igem/BBa_K086018/annotation1995752/range1995752/1
type: Range
persistent identity: range1995752
display id: range1995752
version: 1
direction: direction/forward
top level: BBa_K086018
start: 1
end: 55
orientation: inline

U049O11+D0
type: ComponentDefinition
persistent identity: BBa I732442
display id: BBa_I732442
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Family Member (U049O11+D062O22)
creator: Zhan Jian
created: 2007-10-13T11:00:00Z
modified: 2015-08-31T04:07:58Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732442_sequence/1

const.
type: ComponentDefinition
persistent identity: BBa R1074
display id: BBa_R1074
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Constitutive Promoter I
creator: Chris Walsh (Fighting Darwins)
created: 2004-01-27T12:00:00Z
modified: 2015-05-08T01:14:15Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: constitutive promoter which outputs .3 TIPs point mutants of wild type rrnB promoter P1
mutable provenance: Gaal, Gourse, et al.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -10
sequence annotation: -35
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_R1074_sequence/1

-10
type: SequenceAnnotation
persistent identity: annotation309011
display id: annotation309011
version: 1
top level: const.
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R1074/annotation309011/range309011/1

https://synbiohub.org/public/igem/BBa_R1074/annotation309011/range309011/1
type: Range
persistent identity: range309011
display id: range309011
version: 1
direction: direction/forward
top level: const.
start: 35
end: 40
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation309012
display id: annotation309012
version: 1
top level: const.
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R1074/annotation309012/range309012/1

https://synbiohub.org/public/igem/BBa_R1074/annotation309012/range309012/1
type: Range
persistent identity: range309012
display id: range309012
version: 1
direction: direction/forward
top level: const.
start: 15
end: 20
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2214002
display id: annotation2214002
version: 1
top level: const.
role: SO:0000807
role: feature/barcode
location: https://synbiohub.org/public/igem/BBa_R1074/annotation2214002/range2214002/1

https://synbiohub.org/public/igem/BBa_R1074/annotation2214002/range2214002/1
type: Range
persistent identity: range2214002
display id: range2214002
version: 1
direction: direction/forward
top level: const.
start: 50
end: 74
orientation: inline

BBa_K256033
type: ComponentDefinition
persistent identity: BBa K256033
display id: BBa_K256033
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Infrared signal reporter (J23119:IFP:J23119:HO1)
creator: iGEM09_NTU-Singapore
created: 2009-10-19T11:00:00Z
modified: 2015-05-08T01:11:41Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _359_
m user id: 0
owner id: 4864
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This part co-expresses IFP and HO1.
mutable notes: --
mutable provenance: Gene sequence is obtained directly from Tsien's lab.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_K256020
component: BBa_K256018
sequence annotation: BBa_K256018
sequence annotation: BBa_K256020
sequence: https://synbiohub.org/public/igem/BBa_K256033_sequence/1

BBa_K256020
type: Component
persistent identity: component2288213
display id: component2288213
version: 1
top level: BBa_K256033
access: public
definition: BBa_K256020

BBa_K256018
type: Component
persistent identity: component2288200
display id: component2288200
version: 1
top level: BBa_K256033
access: public
definition: BBa_K256018

BBa_K256018
type: SequenceAnnotation
persistent identity: annotation2288200
display id: annotation2288200
version: 1
top level: BBa_K256033
location: https://synbiohub.org/public/igem/BBa_K256033/annotation2288200/range2288200/1
component: BBa_K256018

https://synbiohub.org/public/igem/BBa_K256033/annotation2288200/range2288200/1
type: Range
persistent identity: range2288200
display id: range2288200
version: 1
direction: direction/reverse
top level: BBa_K256033
start: 1
end: 1167
orientation: inline

BBa_K256020
type: SequenceAnnotation
persistent identity: annotation2288213
display id: annotation2288213
version: 1
top level: BBa_K256033
location: https://synbiohub.org/public/igem/BBa_K256033/annotation2288213/range2288213/1
component: BBa_K256020

https://synbiohub.org/public/igem/BBa_K256033/annotation2288213/range2288213/1
type: Range
persistent identity: range2288213
display id: range2288213
version: 1
direction: direction/reverse
top level: BBa_K256033
start: 1176
end: 2124
orientation: inline

Pnot(O24)
type: ComponentDefinition
persistent identity: BBa I732274
display id: BBa_I732274
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Family Member with Hybrid Operator (D001O24)
creator: Zhan Jian
created: 2007-10-13T11:00:00Z
modified: 2015-08-31T04:07:57Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732274_sequence/1

BBa_J61123
type: ComponentDefinition
persistent identity: BBa J61123
display id: BBa_J61123
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Ribosome Binding Site Family Member
creator: John Anderson
created: 2007-04-24T11:00:00Z
modified: 2015-08-31T01:59:49Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: false
mutable description: {{JCA_Arkin_RBSFamily}}
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J61123_sequence/1

BBa_B0074
type: ComponentDefinition
persistent identity: BBa B0074
display id: BBa_B0074
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Orthogonal RBS
creator: Bartholomew Canton
created: 2006-05-01T11:00:00Z
modified: 2015-08-31T04:07:21Z
discontinued: false
dominant: true
experience: experience/None
group u list: _11_6_
m user id: 0
owner id: 135
owning group id: 6
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_B0074_sequence/1

BBa_K1170000
type: ComponentDefinition
persistent identity: BBa K1170000
display id: BBa_K1170000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: asr (acid shock response) promoter
creator: Rohit Satija
created: 2013-09-01T11:00:00Z
modified: 2015-05-08T01:09:34Z
discontinued: false
dominant: false
group u list: _1483_
m user id: 0
owner id: 16731
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: An acid inducible promoter of the acid shock response (asr) gene, extracted from E. coli K12. This part can be used to generate pH based response in any biological system.
mutable notes: The sequence provided in literature indicated a consensus sequence for the Pho Box just before the transcription start site
mutable provenance: This part was extracted from the genome of E. coli K12
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: Pho Box
sequence annotation: asr promoter
sequence annotation: TATA box
sequence: https://synbiohub.org/public/igem/BBa_K1170000_sequence/1

Pho Box
type: SequenceAnnotation
persistent identity: annotation2334964
display id: annotation2334964
version: 1
top level: BBa_K1170000
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K1170000/annotation2334964/range2334964/1

https://synbiohub.org/public/igem/BBa_K1170000/annotation2334964/range2334964/1
type: Range
persistent identity: range2334964
display id: range2334964
version: 1
direction: direction/forward
top level: BBa_K1170000
start: 114
end: 131
orientation: inline

asr promoter
type: SequenceAnnotation
persistent identity: annotation2334962
display id: annotation2334962
version: 1
top level: BBa_K1170000
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1170000/annotation2334962/range2334962/1

https://synbiohub.org/public/igem/BBa_K1170000/annotation2334962/range2334962/1
type: Range
persistent identity: range2334962
display id: range2334962
version: 1
direction: direction/forward
top level: BBa_K1170000
start: 1
end: 178
orientation: inline

TATA box
type: SequenceAnnotation
persistent identity: annotation2334963
display id: annotation2334963
version: 1
top level: BBa_K1170000
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K1170000/annotation2334963/range2334963/1

https://synbiohub.org/public/igem/BBa_K1170000/annotation2334963/range2334963/1
type: Range
persistent identity: range2334963
display id: range2334963
version: 1
direction: direction/forward
top level: BBa_K1170000
start: 141
end: 147
orientation: inline

U097O11+D0
type: ComponentDefinition
persistent identity: BBa I732418
display id: BBa_I732418
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Family Member (U097O11+D062O22)
creator: Zhan Jian
created: 2007-10-13T11:00:00Z
modified: 2015-08-31T04:07:58Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732418_sequence/1

BBa_K1824892
type: ComponentDefinition
persistent identity: BBa K1824892
display id: BBa_K1824892
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: J23111+RBS
creator: Zixu Wang
created: 2015-09-09T11:00:00Z
modified: 2015-09-11T08:34:06Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2250_
m user id: 26000
owner id: 26000
owning group id: 9
status: status/Planning
bookmark: false
mutable description: A regulatory part composed of a promotor and an RBS, can be used for both transcription and translation. Just add any protein after it.
mutable notes: No special concern
mutable provenance: J23111 is from constitutive promoter family and B0034 is an RBS based on Elowitz repressilator
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_J23111
component: BBa_B0034
component: BBa_K1824890
component: BBa_K1824889
sequence annotation: BBa_B0034
sequence annotation: BBa_K1824890
sequence annotation: BBa_J23111
sequence annotation: BBa_K1824889
sequence: https://synbiohub.org/public/igem/BBa_K1824892_sequence/1

BBa_J23111
type: Component
persistent identity: component2450193
display id: component2450193
version: 1
top level: BBa_K1824892
access: public
definition: BBa_J23111

BBa_B0034
type: Component
persistent identity: component2450196
display id: component2450196
version: 1
top level: BBa_K1824892
access: public
definition: BBa_B0034

BBa_K1824890
type: Component
persistent identity: component2450197
display id: component2450197
version: 1
top level: BBa_K1824892
access: public
definition: https://synbiohub.org/public/igem/BBa_K1824890/1

BBa_K1824889
type: Component
persistent identity: component2450194
display id: component2450194
version: 1
top level: BBa_K1824892
access: public
definition: https://synbiohub.org/public/igem/BBa_K1824889/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2450196
display id: annotation2450196
version: 1
top level: BBa_K1824892
location: https://synbiohub.org/public/igem/BBa_K1824892/annotation2450196/range2450196/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1824892/annotation2450196/range2450196/1
type: Range
persistent identity: range2450196
display id: range2450196
version: 1
direction: direction/reverse
top level: BBa_K1824892
start: 44
end: 55
orientation: inline

BBa_K1824890
type: SequenceAnnotation
persistent identity: annotation2450197
display id: annotation2450197
version: 1
top level: BBa_K1824892
location: https://synbiohub.org/public/igem/BBa_K1824892/annotation2450197/range2450197/1
component: BBa_K1824890

https://synbiohub.org/public/igem/BBa_K1824892/annotation2450197/range2450197/1
type: Range
persistent identity: range2450197
display id: range2450197
version: 1
direction: direction/reverse
top level: BBa_K1824892
start: 56
end: 61
orientation: inline

BBa_J23111
type: SequenceAnnotation
persistent identity: annotation2450193
display id: annotation2450193
version: 1
top level: BBa_K1824892
location: https://synbiohub.org/public/igem/BBa_K1824892/annotation2450193/range2450193/1
component: BBa_J23111

https://synbiohub.org/public/igem/BBa_K1824892/annotation2450193/range2450193/1
type: Range
persistent identity: range2450193
display id: range2450193
version: 1
direction: direction/reverse
top level: BBa_K1824892
start: 1
end: 35
orientation: inline

BBa_K1824889
type: SequenceAnnotation
persistent identity: annotation2450194
display id: annotation2450194
version: 1
top level: BBa_K1824892
location: https://synbiohub.org/public/igem/BBa_K1824892/annotation2450194/range2450194/1
component: BBa_K1824889

https://synbiohub.org/public/igem/BBa_K1824892/annotation2450194/range2450194/1
type: Range
persistent identity: range2450194
display id: range2450194
version: 1
direction: direction/reverse
top level: BBa_K1824892
start: 36
end: 43
orientation: inline

BBa_J07019
type: ComponentDefinition
persistent identity: BBa J07019
display id: BBa_J07019
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: FecA Promoter (with Fur box)
creator: jxyu
created: 2005-07-12T11:00:00Z
modified: 2015-08-31T04:08:19Z
discontinued: false
dominant: true
experience: experience/None
group u list: _21_
m user id: 0
owner id: 384
owning group id: 21
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: In native e.coli, this promoter is located upstream of FecA cds. Regulated by FecI protein, which is in turn regulated by FecA.
mutable provenance: MG1655
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: Fur Box (binds Fur)
sequence annotation: -59 to -1 region
sequence annotation: FecI binding region
sequence: https://synbiohub.org/public/igem/BBa_J07019_sequence/1

Fur Box (binds Fur)
type: SequenceAnnotation
persistent identity: annotation1579238
display id: annotation1579238
version: 1
top level: BBa_J07019
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_J07019/annotation1579238/range1579238/1

https://synbiohub.org/public/igem/BBa_J07019/annotation1579238/range1579238/1
type: Range
persistent identity: range1579238
display id: range1579238
version: 1
direction: direction/forward
top level: BBa_J07019
start: 25
end: 39
orientation: inline

-59 to -1 region
type: SequenceAnnotation
persistent identity: annotation1579189
display id: annotation1579189
version: 1
top level: BBa_J07019
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J07019/annotation1579189/range1579189/1

https://synbiohub.org/public/igem/BBa_J07019/annotation1579189/range1579189/1
type: Range
persistent identity: range1579189
display id: range1579189
version: 1
direction: direction/forward
top level: BBa_J07019
start: 1
end: 60
orientation: inline

FecI binding region
type: SequenceAnnotation
persistent identity: annotation1579239
display id: annotation1579239
version: 1
top level: BBa_J07019
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_J07019/annotation1579239/range1579239/1

https://synbiohub.org/public/igem/BBa_J07019/annotation1579239/range1579239/1
type: Range
persistent identity: range1579239
display id: range1579239
version: 1
direction: direction/forward
top level: BBa_J07019
start: 70
end: 76
orientation: inline

BBa_K266007
type: ComponentDefinition
persistent identity: BBa K266007
display id: BBa_K266007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Complex QS -> LuxI & LasI circuit
creator: UNAM FCIENCIAS-CINVESTAV IRA
created: 2009-10-12T11:00:00Z
modified: 2015-05-08T01:11:43Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _363_
m user id: 0
owner id: 1955
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: Complex Quorum sensing circuit that receives the signal of PAI+LasR and AI+LuxR to control the production of LuxI and LasI enzymes.
mutable notes: None
mutable provenance: The biobricks BBa_K266006 and BBa_K266000 where used to build this biobrick
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Signalling
role: SO:0000804
component: BBa_K266000
component: BBa_K266006
sequence annotation: BBa_K266000
sequence annotation: BBa_K266006
sequence: https://synbiohub.org/public/igem/BBa_K266007_sequence/1

BBa_K266000
type: Component
persistent identity: component2297452
display id: component2297452
version: 1
top level: BBa_K266007
access: public
definition: BBa_K266000

BBa_K266006
type: Component
persistent identity: component2297433
display id: component2297433
version: 1
top level: BBa_K266007
access: public
definition: BBa_K266006

BBa_K266000
type: SequenceAnnotation
persistent identity: annotation2297452
display id: annotation2297452
version: 1
top level: BBa_K266007
location: https://synbiohub.org/public/igem/BBa_K266007/annotation2297452/range2297452/1
component: BBa_K266000

https://synbiohub.org/public/igem/BBa_K266007/annotation2297452/range2297452/1
type: Range
persistent identity: range2297452
display id: range2297452
version: 1
direction: direction/reverse
top level: BBa_K266007
start: 1714
end: 2676
orientation: inline

BBa_K266006
type: SequenceAnnotation
persistent identity: annotation2297433
display id: annotation2297433
version: 1
top level: BBa_K266007
location: https://synbiohub.org/public/igem/BBa_K266007/annotation2297433/range2297433/1
component: BBa_K266006

https://synbiohub.org/public/igem/BBa_K266007/annotation2297433/range2297433/1
type: Range
persistent identity: range2297433
display id: range2297433
version: 1
direction: direction/reverse
top level: BBa_K266007
start: 1
end: 1705
orientation: inline

BBa_K1486043
type: ComponentDefinition
persistent identity: BBa K1486043
display id: BBa_K1486043
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Leucine Zipper + split rLuc
creator: EPFL iGem team 2014
created: 2014-10-01T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: To edit. Pense ?? rajouter les linkers, je ne sais pas s'ils sont de igem ou s'il faut ajouter la s??quence ?? la registry. Check pour tous tes biobricks et dis-moi, please.
mutable notes: To edit
mutable provenance: To edit
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K1486019
component: BBa_K1486042
component: BBa_B0034
component: BBa_K1486020
component: BBa_I0500
sequence annotation: BBa_K1486019
sequence annotation: BBa_K1486042
sequence annotation: BBa_B0034
sequence annotation: BBa_K1486020
sequence annotation: BBa_I0500
sequence: https://synbiohub.org/public/igem/BBa_K1486043_sequence/1

BBa_K1486019
type: Component
persistent identity: component2392174
display id: component2392174
version: 1
top level: BBa_K1486043
access: public
definition: BBa_K1486019

BBa_K1486042
type: Component
persistent identity: component2392177
display id: component2392177
version: 1
top level: BBa_K1486043
access: public
definition: BBa_K1486042

BBa_B0034
type: Component
persistent identity: component2392176
display id: component2392176
version: 1
top level: BBa_K1486043
access: public
definition: BBa_B0034

BBa_B0034
type: Component
persistent identity: component2392172
display id: component2392172
version: 1
top level: BBa_K1486043
access: public
definition: BBa_B0034

BBa_K1486020
type: Component
persistent identity: component2392178
display id: component2392178
version: 1
top level: BBa_K1486043
access: public
definition: BBa_K1486020

BBa_K1486042
type: Component
persistent identity: component2392173
display id: component2392173
version: 1
top level: BBa_K1486043
access: public
definition: BBa_K1486042

BBa_I0500
type: Component
persistent identity: component2392170
display id: component2392170
version: 1
top level: BBa_K1486043
access: public
definition: pBad/araC

BBa_K1486019
type: SequenceAnnotation
persistent identity: annotation2392174
display id: annotation2392174
version: 1
top level: BBa_K1486043
location: https://synbiohub.org/public/igem/BBa_K1486043/annotation2392174/range2392174/1
component: BBa_K1486019

https://synbiohub.org/public/igem/BBa_K1486043/annotation2392174/range2392174/1
type: Range
persistent identity: range2392174
display id: range2392174
version: 1
direction: direction/reverse
top level: BBa_K1486043
start: 1373
end: 1702
orientation: inline

BBa_K1486042
type: SequenceAnnotation
persistent identity: annotation2392177
display id: annotation2392177
version: 1
top level: BBa_K1486043
location: https://synbiohub.org/public/igem/BBa_K1486043/annotation2392177/range2392177/1
component: BBa_K1486042

https://synbiohub.org/public/igem/BBa_K1486043/annotation2392177/range2392177/1
type: Range
persistent identity: range2392177
display id: range2392177
version: 1
direction: direction/reverse
top level: BBa_K1486043
start: 1715
end: 1864
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2392172
display id: annotation2392172
version: 1
top level: BBa_K1486043
location: https://synbiohub.org/public/igem/BBa_K1486043/annotation2392172/range2392172/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1486043/annotation2392172/range2392172/1
type: Range
persistent identity: range2392172
display id: range2392172
version: 1
direction: direction/reverse
top level: BBa_K1486043
start: 1211
end: 1222
orientation: inline

BBa_K1486020
type: SequenceAnnotation
persistent identity: annotation2392178
display id: annotation2392178
version: 1
top level: BBa_K1486043
location: https://synbiohub.org/public/igem/BBa_K1486043/annotation2392178/range2392178/1
component: BBa_K1486020

https://synbiohub.org/public/igem/BBa_K1486043/annotation2392178/range2392178/1
type: Range
persistent identity: range2392178
display id: range2392178
version: 1
direction: direction/reverse
top level: BBa_K1486043
start: 1865
end: 2470
orientation: inline

BBa_K1486042
type: SequenceAnnotation
persistent identity: annotation2392173
display id: annotation2392173
version: 1
top level: BBa_K1486043
location: https://synbiohub.org/public/igem/BBa_K1486043/annotation2392173/range2392173/1
component: BBa_K1486042

https://synbiohub.org/public/igem/BBa_K1486043/annotation2392173/range2392173/1
type: Range
persistent identity: range2392173
display id: range2392173
version: 1
direction: direction/reverse
top level: BBa_K1486043
start: 1223
end: 1372
orientation: inline

BBa_I0500
type: SequenceAnnotation
persistent identity: annotation2392170
display id: annotation2392170
version: 1
top level: BBa_K1486043
location: https://synbiohub.org/public/igem/BBa_K1486043/annotation2392170/range2392170/1
component: BBa_I0500

https://synbiohub.org/public/igem/BBa_K1486043/annotation2392170/range2392170/1
type: Range
persistent identity: range2392170
display id: range2392170
version: 1
direction: direction/reverse
top level: BBa_K1486043
start: 1
end: 1210
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2392176
display id: annotation2392176
version: 1
top level: BBa_K1486043
location: https://synbiohub.org/public/igem/BBa_K1486043/annotation2392176/range2392176/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1486043/annotation2392176/range2392176/1
type: Range
persistent identity: range2392176
display id: range2392176
version: 1
direction: direction/reverse
top level: BBa_K1486043
start: 1703
end: 1714
orientation: inline

BBa_K1486002
type: ComponentDefinition
persistent identity: BBa K1486002
display id: BBa_K1486002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Arabinose promoter + sfGFP CpxR (Nterm)
creator: iGem EPFL 2014
created: 2014-08-11T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part is composed of an arabinose-inducible promoter for superfolded GFP attached by a linker to CpxR (on the N terminus of CpxR).
mutable notes: We had to test both attachments, N and C terminus of the CpxR.
mutable provenance: The arabinose promoter and the sfGFP were obtained from the registry, while the CpxR was obtained from the E coli genome.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0034
component: BBa_K1486003
component: BBa_K1486000
component: BBa_I746916
component: BBa_I0500
sequence annotation: BBa_K1486003
sequence annotation: BBa_I746916
sequence annotation: BBa_I0500
sequence annotation: BBa_K1486000
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K1486002_sequence/1

BBa_B0034
type: Component
persistent identity: component2382059
display id: component2382059
version: 1
top level: BBa_K1486002
access: public
definition: BBa_B0034

BBa_K1486003
type: Component
persistent identity: component2382064
display id: component2382064
version: 1
top level: BBa_K1486002
access: public
definition: BBa_K1486003

BBa_K1486000
type: Component
persistent identity: component2382068
display id: component2382068
version: 1
top level: BBa_K1486002
access: public
definition: BBa_K1486000

BBa_I746916
type: Component
persistent identity: component2382063
display id: component2382063
version: 1
top level: BBa_K1486002
access: public
definition: https://synbiohub.org/public/igem/BBa_I746916/1

BBa_I0500
type: Component
persistent identity: component2382057
display id: component2382057
version: 1
top level: BBa_K1486002
access: public
definition: pBad/araC

BBa_K1486003
type: SequenceAnnotation
persistent identity: annotation2382064
display id: annotation2382064
version: 1
top level: BBa_K1486002
location: https://synbiohub.org/public/igem/BBa_K1486002/annotation2382064/range2382064/1
component: BBa_K1486003

https://synbiohub.org/public/igem/BBa_K1486002/annotation2382064/range2382064/1
type: Range
persistent identity: range2382064
display id: range2382064
version: 1
direction: direction/reverse
top level: BBa_K1486002
start: 1943
end: 1966
orientation: inline

BBa_I746916
type: SequenceAnnotation
persistent identity: annotation2382063
display id: annotation2382063
version: 1
top level: BBa_K1486002
location: https://synbiohub.org/public/igem/BBa_K1486002/annotation2382063/range2382063/1
component: BBa_I746916

https://synbiohub.org/public/igem/BBa_K1486002/annotation2382063/range2382063/1
type: Range
persistent identity: range2382063
display id: range2382063
version: 1
direction: direction/reverse
top level: BBa_K1486002
start: 1223
end: 1942
orientation: inline

BBa_I0500
type: SequenceAnnotation
persistent identity: annotation2382057
display id: annotation2382057
version: 1
top level: BBa_K1486002
location: https://synbiohub.org/public/igem/BBa_K1486002/annotation2382057/range2382057/1
component: BBa_I0500

https://synbiohub.org/public/igem/BBa_K1486002/annotation2382057/range2382057/1
type: Range
persistent identity: range2382057
display id: range2382057
version: 1
direction: direction/reverse
top level: BBa_K1486002
start: 1
end: 1210
orientation: inline

BBa_K1486000
type: SequenceAnnotation
persistent identity: annotation2382068
display id: annotation2382068
version: 1
top level: BBa_K1486002
location: https://synbiohub.org/public/igem/BBa_K1486002/annotation2382068/range2382068/1
component: BBa_K1486000

https://synbiohub.org/public/igem/BBa_K1486002/annotation2382068/range2382068/1
type: Range
persistent identity: range2382068
display id: range2382068
version: 1
direction: direction/reverse
top level: BBa_K1486002
start: 1967
end: 2668
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2382059
display id: annotation2382059
version: 1
top level: BBa_K1486002
location: https://synbiohub.org/public/igem/BBa_K1486002/annotation2382059/range2382059/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1486002/annotation2382059/range2382059/1
type: Range
persistent identity: range2382059
display id: range2382059
version: 1
direction: direction/reverse
top level: BBa_K1486002
start: 1211
end: 1222
orientation: inline

BBa_K112706
type: ComponentDefinition
persistent identity: BBa K112706
display id: BBa_K112706
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Pspv2 from Salmonella
creator: Bing Xia
created: 2008-10-20T11:00:00Z
modified: 2015-05-08T01:09:18Z
discontinued: false
dominant: false
experience: experience/None
group u list: _224_
m user id: 0
owner id: 2998
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: The spv2 promoter. ---- This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at: [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
mutable provenance: Construction of <Pspv2> basic part K112706 PCR of dv015/dv016 on pBca1037 (500bp, EcoRI/BamHI/DpnI) Sub into pBca1256 (EcoRI/BamHI, 2472+ 500, L) Product is pBca1256-K112706 ----------------------------------------------- dv015 Forward biobricking of <Pspv2> cgataGAATTCatgAGATCTccttatgcagcgtaagggccgcaac dv016 Reverse biobricking of <Pspv2> cgttaGGATCCGATAATGTtTGCAGGGGAATTATTTTG
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K112706_sequence/1

BBa_I746360
type: ComponentDefinition
persistent identity: BBa I746360
display id: BBa_I746360
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PF promoter from P2 phage
creator: Stefan Milde
created: 2007-09-10T11:00:00Z
modified: 2015-08-31T04:08:04Z
discontinued: false
dominant: false
experience: experience/None
group u list: _116_
m user id: 0
owner id: 2122
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This is the PF promoter taken from the P2 phage genome. It is an inducible promoter that is activated by a class of activators, including P2 ogr (I746350), PSP3 pag (I746351) and phiR73 delta (I746352). These different activators should cause different levels of activity of the PF promoter.
mutable notes: no special considerations seemed necessary
mutable provenance: The original source is the P2 phage genome. For our work the DNA came from plasmids, kindly supplied to us by Prof. Calendar, University of California (Berkeley): Bacteriophage PSP3 and fR73 Activator Proteins: Analysis of Promoter Specificities; Julien and Calendar, 1996; JOURNAL OF BACTERIOLOGY, Oct. 1996, p. 5668???5675
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: PF
sequence: https://synbiohub.org/public/igem/BBa_I746360_sequence/1

PF
type: SequenceAnnotation
persistent identity: annotation1943783
display id: annotation1943783
version: 1
top level: BBa_I746360
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I746360/annotation1943783/range1943783/1

https://synbiohub.org/public/igem/BBa_I746360/annotation1943783/range1943783/1
type: Range
persistent identity: range1943783
display id: range1943783
version: 1
direction: direction/forward
top level: BBa_I746360
start: 1
end: 91
orientation: inline

BBa_J61138
type: ComponentDefinition
persistent identity: BBa J61138
display id: BBa_J61138
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Ribosome Binding Site Family Member
creator: John Anderson
created: 2007-04-24T11:00:00Z
modified: 2015-08-31T01:59:49Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: false
mutable description: {{JCA_Arkin_RBSFamily}}
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J61138_sequence/1

Pnot(O66)
type: ComponentDefinition
persistent identity: BBa I732206
display id: BBa_I732206
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: NOT Gate Promoter Family Member (D001O66)
creator: Zhan Jian
created: 2007-10-13T11:00:00Z
modified: 2015-08-31T04:07:57Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732206_sequence/1

BBa_J05209
type: ComponentDefinition
persistent identity: BBa J05209
display id: BBa_J05209
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Modifed Pr Promoter
creator: Alexander Roth
created: 2005-09-14T11:00:00Z
modified: 2015-08-31T04:08:15Z
discontinued: false
dominant: false
group u list: _34_
m user id: 0
owner id: 412
owning group id: 34
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This is identical to BBa_R0051 exept that some base has been modifed to alow for additional ZFP repressors to bind
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -35
sequence annotation: OR2
sequence annotation: ZFP binding sites
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_J05209_sequence/1

-35
type: SequenceAnnotation
persistent identity: annotation1689811
display id: annotation1689811
version: 1
top level: BBa_J05209
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_J05209/annotation1689811/range1689811/1

https://synbiohub.org/public/igem/BBa_J05209/annotation1689811/range1689811/1
type: Range
persistent identity: range1689811
display id: range1689811
version: 1
direction: direction/forward
top level: BBa_J05209
start: 15
end: 20
orientation: inline

OR2
type: SequenceAnnotation
persistent identity: annotation1689809
display id: annotation1689809
version: 1
top level: BBa_J05209
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_J05209/annotation1689809/range1689809/1

https://synbiohub.org/public/igem/BBa_J05209/annotation1689809/range1689809/1
type: Range
persistent identity: range1689809
display id: range1689809
version: 1
direction: direction/forward
top level: BBa_J05209
start: 1
end: 17
orientation: inline

ZFP binding sites
type: SequenceAnnotation
persistent identity: annotation1689899
display id: annotation1689899
version: 1
top level: BBa_J05209
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_J05209/annotation1689899/range1689899/1

https://synbiohub.org/public/igem/BBa_J05209/annotation1689899/range1689899/1
type: Range
persistent identity: range1689899
display id: range1689899
version: 1
direction: direction/forward
top level: BBa_J05209
start: 18
end: 29
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1689812
display id: annotation1689812
version: 1
top level: BBa_J05209
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_J05209/annotation1689812/range1689812/1

https://synbiohub.org/public/igem/BBa_J05209/annotation1689812/range1689812/1
type: Range
persistent identity: range1689812
display id: range1689812
version: 1
direction: direction/forward
top level: BBa_J05209
start: 38
end: 43
orientation: inline

OR2
type: SequenceAnnotation
persistent identity: annotation1689810
display id: annotation1689810
version: 1
top level: BBa_J05209
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_J05209/annotation1689810/range1689810/1

https://synbiohub.org/public/igem/BBa_J05209/annotation1689810/range1689810/1
type: Range
persistent identity: range1689810
display id: range1689810
version: 1
direction: direction/forward
top level: BBa_J05209
start: 25
end: 41
orientation: inline

BBa_K136001
type: ComponentDefinition
persistent identity: BBa K136001
display id: BBa_K136001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter of flgA gene from E. coli flagella
creator: Cyprien Maisonnier
created: 2008-08-11T11:00:00Z
modified: 2015-05-08T01:10:02Z
discontinued: true
dominant: false
experience: experience/None
group u list: _211_
m user id: 0
owner id: 3012
owning group id: 9
sample status: Discontinued
status: status/Deleted
bookmark: false
mutable description: ''fliA'' is a class 2 gene that is part of the construction of the flagella ( ''Ordering Genes in a Flagella Pathway by Analysis of Expression Kinetics from Living Bacteria'' - S. Kalir, J. McClure, K. Pabbaraju, C. Southward, M. Ronen, S. Leibler, M. G. Surette, U. Alon ). Its promoter is consitutively repressed. It is activated by FlhD4C2 and maybe by FliA with different strength ( ''Using a Quantitative Blueprint to Reprogram the Dynamics of the Flagella Gene Network'' - Shiraz Kalir and Uri Alon ). '''Work in progress :''' Deeper quantitative characterization
mutable notes: The primers used to amplify this sequence are : *Forward : GTTTCTTCGAATTCGCGGCCGCTTCTAGAGAGCATATCTCCTCCGCAGGTATCAAAAT *Reverse : GTTTCTTCCTGCAGCGGCCGCTACTAGTAACAGTATCGCGATGATCGCCACGCTACGT
mutable provenance: This part comes from the genome of ''E.coli'' K12 strain MG1655.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: FlhDC binding site 1
sequence annotation: Initiation start for transcription
sequence annotation: FlhDC binding site 2
sequence: https://synbiohub.org/public/igem/BBa_K136001_sequence/1

FlhDC binding site 1
type: SequenceAnnotation
persistent identity: annotation1971568
display id: annotation1971568
version: 1
top level: BBa_K136001
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K136001/annotation1971568/range1971568/1

https://synbiohub.org/public/igem/BBa_K136001/annotation1971568/range1971568/1
type: Range
persistent identity: range1971568
display id: range1971568
version: 1
direction: direction/forward
top level: BBa_K136001
start: 63
end: 78
orientation: inline

Initiation start for transcription
type: SequenceAnnotation
persistent identity: annotation1971570
display id: annotation1971570
version: 1
top level: BBa_K136001
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K136001/annotation1971570/range1971570/1

https://synbiohub.org/public/igem/BBa_K136001/annotation1971570/range1971570/1
type: Range
persistent identity: range1971570
display id: range1971570
version: 1
direction: direction/forward
top level: BBa_K136001
start: 138
end: 138
orientation: inline

FlhDC binding site 2
type: SequenceAnnotation
persistent identity: annotation1971569
display id: annotation1971569
version: 1
top level: BBa_K136001
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K136001/annotation1971569/range1971569/1

https://synbiohub.org/public/igem/BBa_K136001/annotation1971569/range1971569/1
type: Range
persistent identity: range1971569
display id: range1971569
version: 1
direction: direction/forward
top level: BBa_K136001
start: 69
end: 104
orientation: inline

pJEJAM11
type: ComponentDefinition
persistent identity: BBa K678059
display id: BBa_K678059
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pJEJAM11
creator: DTU-Denmark-2
created: 2011-09-12T11:00:00Z
modified: 2015-05-08T01:13:05Z
discontinued: false
dominant: false
experience: experience/None
group u list: _882_
m user id: 0
owner id: 8341
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: To be announced
mutable notes: To be announced
mutable provenance: To be announced
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000155
role: partType/Plasmid
component: BBa_K678037
component: BBa_K678026
component: BBa_K678001
component: BBa_K678045
sequence annotation: BBa_K678045
sequence annotation: BBa_K678001
sequence annotation: BBa_K678037
sequence annotation: BBa_K678026
sequence: https://synbiohub.org/public/igem/BBa_K678059_sequence/1

BBa_K678037
type: Component
persistent identity: component2127477
display id: component2127477
version: 1
top level: pJEJAM11
access: public
definition: BBa_K678037

BBa_K678026
type: Component
persistent identity: component2127474
display id: component2127474
version: 1
top level: pJEJAM11
access: public
definition: https://synbiohub.org/public/igem/BBa_K678026/1

BBa_K678001
type: Component
persistent identity: component2127471
display id: component2127471
version: 1
top level: pJEJAM11
access: public
definition: PalcA

BBa_K678045
type: Component
persistent identity: component2127480
display id: component2127480
version: 1
top level: pJEJAM11
access: public
definition: ptrA

BBa_K678045
type: SequenceAnnotation
persistent identity: annotation2127480
display id: annotation2127480
version: 1
top level: pJEJAM11
location: https://synbiohub.org/public/igem/BBa_K678059/annotation2127480/range2127480/1
component: BBa_K678045

https://synbiohub.org/public/igem/BBa_K678059/annotation2127480/range2127480/1
type: Range
persistent identity: range2127480
display id: range2127480
version: 1
direction: direction/reverse
top level: pJEJAM11
start: 4957
end: 6855
orientation: inline

BBa_K678001
type: SequenceAnnotation
persistent identity: annotation2127471
display id: annotation2127471
version: 1
top level: pJEJAM11
location: https://synbiohub.org/public/igem/BBa_K678059/annotation2127471/range2127471/1
component: BBa_K678001

https://synbiohub.org/public/igem/BBa_K678059/annotation2127471/range2127471/1
type: Range
persistent identity: range2127471
display id: range2127471
version: 1
direction: direction/reverse
top level: pJEJAM11
start: 1
end: 843
orientation: inline

BBa_K678037
type: SequenceAnnotation
persistent identity: annotation2127477
display id: annotation2127477
version: 1
top level: pJEJAM11
location: https://synbiohub.org/public/igem/BBa_K678059/annotation2127477/range2127477/1
component: BBa_K678037

https://synbiohub.org/public/igem/BBa_K678059/annotation2127477/range2127477/1
type: Range
persistent identity: range2127477
display id: range2127477
version: 1
direction: direction/reverse
top level: pJEJAM11
start: 3943
end: 4948
orientation: inline

BBa_K678026
type: SequenceAnnotation
persistent identity: annotation2127474
display id: annotation2127474
version: 1
top level: pJEJAM11
location: https://synbiohub.org/public/igem/BBa_K678059/annotation2127474/range2127474/1
component: BBa_K678026

https://synbiohub.org/public/igem/BBa_K678059/annotation2127474/range2127474/1
type: Range
persistent identity: range2127474
display id: range2127474
version: 1
direction: direction/reverse
top level: pJEJAM11
start: 852
end: 3934
orientation: inline

BBa_K1486053
type: ComponentDefinition
persistent identity: BBa K1486053
display id: BBa_K1486053
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: 10 aa linker
creator: EPFL iGem team 2014
created: 2014-10-06T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: to edit
mutable notes: to edit
mutable provenance: to edit
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence: https://synbiohub.org/public/igem/BBa_K1486053_sequence/1

BBa_K165002
type: ComponentDefinition
persistent identity: BBa K165002
display id: BBa_K165002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Kozak sequence (yeast RBS)
creator: John Szymanski
created: 2008-10-25T11:00:00Z
modified: 2015-05-08T01:10:55Z
discontinued: false
dominant: false
experience: experience/None
group u list: _267_
m user id: 0
owner id: 2512
owning group id: 58
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: The kozak sequence acts as a eukaryotic RBS. It is cloned directly between a promoter and coding region.
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_K165002_sequence/1

BBa_K110009
type: ComponentDefinition
persistent identity: BBa K110009
display id: BBa_K110009
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: A-Cell Promoter STE2
creator: Rick Carrick, Ambhi Ganesan, Alex McMillan
created: 2008-05-29T11:00:00Z
modified: 2015-05-08T01:09:09Z
discontinued: false
dominant: false
experience: experience/None
group u list: _201_
m user id: 0
owner id: 2669
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This promoter was isolated by taking 500bp upstream of the MFA1 gene of S. cerevisiae. This promoter will be activated when A factor is expressed in an A haploid yeast cell.
mutable notes: This promotor is on the crick strand of the entered sequence.
mutable provenance: SGD
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: MCM1
sequence annotation: Mat Alpha2
sequence annotation: Ste2 Promoter
sequence annotation: STE12
sequence: https://synbiohub.org/public/igem/BBa_K110009_sequence/1

MCM1
type: SequenceAnnotation
persistent identity: annotation1981500
display id: annotation1981500
version: 1
top level: BBa_K110009
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K110009/annotation1981500/range1981500/1

https://synbiohub.org/public/igem/BBa_K110009/annotation1981500/range1981500/1
type: Range
persistent identity: range1981500
display id: range1981500
version: 1
direction: direction/forward
top level: BBa_K110009
start: 283
end: 292
orientation: inline

Mat Alpha2
type: SequenceAnnotation
persistent identity: annotation1981501
display id: annotation1981501
version: 1
top level: BBa_K110009
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K110009/annotation1981501/range1981501/1

https://synbiohub.org/public/igem/BBa_K110009/annotation1981501/range1981501/1
type: Range
persistent identity: range1981501
display id: range1981501
version: 1
direction: direction/forward
top level: BBa_K110009
start: 295
end: 301
orientation: inline

Ste2 Promoter
type: SequenceAnnotation
persistent identity: annotation1981498
display id: annotation1981498
version: 1
top level: BBa_K110009
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K110009/annotation1981498/range1981498/1

https://synbiohub.org/public/igem/BBa_K110009/annotation1981498/range1981498/1
type: Range
persistent identity: range1981498
display id: range1981498
version: 1
direction: direction/forward
top level: BBa_K110009
start: 1
end: 501
orientation: inline

STE12
type: SequenceAnnotation
persistent identity: annotation1981502
display id: annotation1981502
version: 1
top level: BBa_K110009
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K110009/annotation1981502/range1981502/1

https://synbiohub.org/public/igem/BBa_K110009/annotation1981502/range1981502/1
type: Range
persistent identity: range1981502
display id: range1981502
version: 1
direction: direction/forward
top level: BBa_K110009
start: 304
end: 312
orientation: inline

Mat Alpha2
type: SequenceAnnotation
persistent identity: annotation1981499
display id: annotation1981499
version: 1
top level: BBa_K110009
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K110009/annotation1981499/range1981499/1

https://synbiohub.org/public/igem/BBa_K110009/annotation1981499/range1981499/1
type: Range
persistent identity: range1981499
display id: range1981499
version: 1
direction: direction/forward
top level: BBa_K110009
start: 273
end: 280
orientation: inline

BBa_J61107
type: ComponentDefinition
persistent identity: BBa J61107
display id: BBa_J61107
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Ribosome Binding Site Family Member
creator: John Anderson
created: 2007-04-23T11:00:00Z
modified: 2015-08-31T02:03:00Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: false
mutable description: {{JCA_Arkin_RBSFamily}}
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J61107_sequence/1

BBa_K1897000
type: ComponentDefinition
persistent identity: BBa K1897000
display id: BBa_K1897000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: HasA hemophore coding sequence
creator: Choi Yan Ru
created: 2016-10-06T11:00:00Z
modified: 2016-10-09T10:00:00Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2363_
m user id: 29428
owner id: 29428
owning group id: 9
status: status/Planning
bookmark: false
mutable description: c
mutable notes: d
mutable provenance: c
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Hexa histadine tag
sequence annotation: Start codon
sequence annotation: HasA
sequence annotation: Stop codon
sequence: https://synbiohub.org/public/igem/BBa_K1897000_sequence/1

Hexa histadine tag
type: SequenceAnnotation
persistent identity: annotation2488978
display id: annotation2488978
version: 1
top level: BBa_K1897000
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1897000/annotation2488978/range2488978/1

https://synbiohub.org/public/igem/BBa_K1897000/annotation2488978/range2488978/1
type: Range
persistent identity: range2488978
display id: range2488978
version: 1
direction: direction/forward
top level: BBa_K1897000
start: 565
end: 582
orientation: inline

Start codon
type: SequenceAnnotation
persistent identity: annotation2488976
display id: annotation2488976
version: 1
top level: BBa_K1897000
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1897000/annotation2488976/range2488976/1

https://synbiohub.org/public/igem/BBa_K1897000/annotation2488976/range2488976/1
type: Range
persistent identity: range2488976
display id: range2488976
version: 1
direction: direction/forward
top level: BBa_K1897000
start: 1
end: 3
orientation: inline

HasA
type: SequenceAnnotation
persistent identity: annotation2488995
display id: annotation2488995
version: 1
top level: BBa_K1897000
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1897000/annotation2488995/range2488995/1

https://synbiohub.org/public/igem/BBa_K1897000/annotation2488995/range2488995/1
type: Range
persistent identity: range2488995
display id: range2488995
version: 1
direction: direction/forward
top level: BBa_K1897000
start: 1
end: 564
orientation: inline

Stop codon
type: SequenceAnnotation
persistent identity: annotation2488977
display id: annotation2488977
version: 1
top level: BBa_K1897000
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1897000/annotation2488977/range2488977/1

https://synbiohub.org/public/igem/BBa_K1897000/annotation2488977/range2488977/1
type: Range
persistent identity: range2488977
display id: range2488977
version: 1
direction: direction/reverse
top level: BBa_K1897000
start: 583
end: 585
orientation: inline

BBa_K1777005
type: ComponentDefinition
persistent identity: BBa K1777005
display id: BBa_K1777005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: flexible linker with SV40 NLS
creator: Xuhang Li
created: 2015-09-12T11:00:00Z
modified: 2015-09-19T11:42:57Z
discontinued: false
dominant: false
experience: experience/Issues
group u list: _2199_
m user id: 27798
owner id: 25645
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This is a 40-50A flexible separation for fusion domains with a SV40 NLS, and its codon is optimized for mammalian. It can be used to link different protein domains for fusion proteins locating in cell nucleus.
mutable notes: The codon is optimized for mammalian, and we insert a NLS to promote locating in cell nucleus. Besides ,we lengthen this linker to provide better separation of two domains. At last we avoid tandem repeat sequence(like GGGSPKKKRKVGGGS), which will facilitate fusing this linker to your domains via overlap PCR.
mutable provenance: This part is improved from BBa_K1486003.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: the second linker
sequence annotation: SV40 NLS
sequence annotation: artificial GS linker
sequence annotation: natural flexible linker
sequence annotation: the first linker
sequence annotation: scar from 3A assembly
sequence: https://synbiohub.org/public/igem/BBa_K1777005_sequence/1

the second linker
type: SequenceAnnotation
persistent identity: annotation2476140
display id: annotation2476140
version: 1
top level: BBa_K1777005
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1777005/annotation2476140/range2476140/1

https://synbiohub.org/public/igem/BBa_K1777005/annotation2476140/range2476140/1
type: Range
persistent identity: range2476140
display id: range2476140
version: 1
direction: direction/forward
top level: BBa_K1777005
start: 68
end: 124
orientation: inline

SV40 NLS
type: SequenceAnnotation
persistent identity: annotation2476142
display id: annotation2476142
version: 1
top level: BBa_K1777005
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1777005/annotation2476142/range2476142/1

https://synbiohub.org/public/igem/BBa_K1777005/annotation2476142/range2476142/1
type: Range
persistent identity: range2476142
display id: range2476142
version: 1
direction: direction/forward
top level: BBa_K1777005
start: 24
end: 44
orientation: inline

artificial GS linker
type: SequenceAnnotation
persistent identity: annotation2476145
display id: annotation2476145
version: 1
top level: BBa_K1777005
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1777005/annotation2476145/range2476145/1

https://synbiohub.org/public/igem/BBa_K1777005/annotation2476145/range2476145/1
type: Range
persistent identity: range2476145
display id: range2476145
version: 1
direction: direction/forward
top level: BBa_K1777005
start: 45
end: 59
orientation: inline

natural flexible linker
type: SequenceAnnotation
persistent identity: annotation2476144
display id: annotation2476144
version: 1
top level: BBa_K1777005
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1777005/annotation2476144/range2476144/1

https://synbiohub.org/public/igem/BBa_K1777005/annotation2476144/range2476144/1
type: Range
persistent identity: range2476144
display id: range2476144
version: 1
direction: direction/forward
top level: BBa_K1777005
start: 3
end: 23
orientation: inline

artificial GS linker
type: SequenceAnnotation
persistent identity: annotation2476147
display id: annotation2476147
version: 1
top level: BBa_K1777005
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1777005/annotation2476147/range2476147/1

https://synbiohub.org/public/igem/BBa_K1777005/annotation2476147/range2476147/1
type: Range
persistent identity: range2476147
display id: range2476147
version: 1
direction: direction/forward
top level: BBa_K1777005
start: 110
end: 124
orientation: inline

SV40 NLS
type: SequenceAnnotation
persistent identity: annotation2476143
display id: annotation2476143
version: 1
top level: BBa_K1777005
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1777005/annotation2476143/range2476143/1

https://synbiohub.org/public/igem/BBa_K1777005/annotation2476143/range2476143/1
type: Range
persistent identity: range2476143
display id: range2476143
version: 1
direction: direction/forward
top level: BBa_K1777005
start: 89
end: 109
orientation: inline

the first linker
type: SequenceAnnotation
persistent identity: annotation2476139
display id: annotation2476139
version: 1
top level: BBa_K1777005
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1777005/annotation2476139/range2476139/1

https://synbiohub.org/public/igem/BBa_K1777005/annotation2476139/range2476139/1
type: Range
persistent identity: range2476139
display id: range2476139
version: 1
direction: direction/forward
top level: BBa_K1777005
start: 3
end: 59
orientation: inline

natural flexible linker
type: SequenceAnnotation
persistent identity: annotation2476146
display id: annotation2476146
version: 1
top level: BBa_K1777005
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1777005/annotation2476146/range2476146/1

https://synbiohub.org/public/igem/BBa_K1777005/annotation2476146/range2476146/1
type: Range
persistent identity: range2476146
display id: range2476146
version: 1
direction: direction/forward
top level: BBa_K1777005
start: 68
end: 88
orientation: inline

scar from 3A assembly
type: SequenceAnnotation
persistent identity: annotation2476141
display id: annotation2476141
version: 1
top level: BBa_K1777005
role: feature/scar
location: https://synbiohub.org/public/igem/BBa_K1777005/annotation2476141/range2476141/1

https://synbiohub.org/public/igem/BBa_K1777005/annotation2476141/range2476141/1
type: Range
persistent identity: range2476141
display id: range2476141
version: 1
direction: direction/forward
top level: BBa_K1777005
start: 60
end: 67
orientation: inline

eCFP-GOI
type: ComponentDefinition
persistent identity: BBa K678048
display id: BBa_K678048
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: eCFP-GOI
creator: DTU-Denmark-2
created: 2011-09-12T11:00:00Z
modified: 2015-05-08T01:13:04Z
discontinued: false
dominant: false
experience: experience/None
group u list: _882_
m user id: 0
owner id: 10556
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part was designed for the Plug'n'Play with DNA assembly standard (RFC 80), therefore this part contains tails at the 5' and 3' ends as shown on the figure below.
mutable notes: No
mutable provenance: The Danish Cancer Society
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: Kosaz
sequence annotation: eCFP-GOI
sequence annotation: Linker 2
sequence: https://synbiohub.org/public/igem/BBa_K678048_sequence/1

Kosaz
type: SequenceAnnotation
persistent identity: annotation2127793
display id: annotation2127793
version: 1
top level: eCFP-GOI
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K678048/annotation2127793/range2127793/1

https://synbiohub.org/public/igem/BBa_K678048/annotation2127793/range2127793/1
type: Range
persistent identity: range2127793
display id: range2127793
version: 1
direction: direction/forward
top level: eCFP-GOI
start: 9
end: 19
orientation: inline

eCFP-GOI
type: SequenceAnnotation
persistent identity: annotation2127669
display id: annotation2127669
version: 1
top level: eCFP-GOI
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K678048/annotation2127669/range2127669/1

https://synbiohub.org/public/igem/BBa_K678048/annotation2127669/range2127669/1
type: Range
persistent identity: range2127669
display id: range2127669
version: 1
direction: direction/forward
top level: eCFP-GOI
start: 16
end: 802
orientation: inline

Linker 2
type: SequenceAnnotation
persistent identity: annotation2127668
display id: annotation2127668
version: 1
top level: eCFP-GOI
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K678048/annotation2127668/range2127668/1

https://synbiohub.org/public/igem/BBa_K678048/annotation2127668/range2127668/1
type: Range
persistent identity: range2127668
display id: range2127668
version: 1
direction: direction/forward
top level: eCFP-GOI
start: 1
end: 8
orientation: inline

U061O11+D0
type: ComponentDefinition
persistent identity: BBa I732432
display id: BBa_I732432
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Family Member (U061O11+D014O22)
creator: Zhan Jian
created: 2007-10-13T11:00:00Z
modified: 2015-08-31T04:07:58Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732432_sequence/1

BBa_K1486038
type: ComponentDefinition
persistent identity: BBa K1486038
display id: BBa_K1486038
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: sfGFPN
creator: EPFL iGem team 2014
created: 2014-09-26T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: To edit
mutable notes: To edit
mutable provenance: To edit
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence: https://synbiohub.org/public/igem/BBa_K1486038_sequence/1

OmpR
type: ComponentDefinition
persistent identity: BBa R0082
display id: BBa_R0082
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter (OmpR, positive)
creator: Stephen Lee, Roshan Kumar, Joe Levine, Ziyan Chu (Polkadorks, IAP 2004)
created: 2004-01-27T12:00:00Z
modified: 2015-05-08T01:14:15Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Positively regulated, OmpR-controlled promoter. This promoter is taken from the upstream region of ompC. Phosphorylated OmpR binds to the three operator sites and activates transcription.
mutable notes: The promoter includes the three OmpR binding sites: C1, C2, and C3. The promoter starts at -107 and goes up to the transcriptional start site at +1. An alternate version, BBa_R0083, cuts out the C2 and C3 sites. The efficacy of this promoter versus the truncated ompC upstream region (BBa_R0083) or the ompF upstream region (BBa_R0084) is currently unknown.
mutable provenance: NC_000193 E. coli K12
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -10
sequence annotation: -35
sequence annotation: C1 OmpR
sequence annotation: C2 OmpR
sequence annotation: C3 OmpR
sequence: https://synbiohub.org/public/igem/BBa_R0082_sequence/1

-10
type: SequenceAnnotation
persistent identity: annotation301167
display id: annotation301167
version: 1
top level: OmpR
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0082/annotation301167/range301167/1

https://synbiohub.org/public/igem/BBa_R0082/annotation301167/range301167/1
type: Range
persistent identity: range301167
display id: range301167
version: 1
direction: direction/forward
top level: OmpR
start: 98
end: 103
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation301166
display id: annotation301166
version: 1
top level: OmpR
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0082/annotation301166/range301166/1

https://synbiohub.org/public/igem/BBa_R0082/annotation301166/range301166/1
type: Range
persistent identity: range301166
display id: range301166
version: 1
direction: direction/forward
top level: OmpR
start: 75
end: 80
orientation: inline

C1 OmpR
type: SequenceAnnotation
persistent identity: annotation301154
display id: annotation301154
version: 1
top level: OmpR
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0082/annotation301154/range301154/1

https://synbiohub.org/public/igem/BBa_R0082/annotation301154/range301154/1
type: Range
persistent identity: range301154
display id: range301154
version: 1
direction: direction/forward
top level: OmpR
start: 13
end: 30
orientation: inline

C2 OmpR
type: SequenceAnnotation
persistent identity: annotation301155
display id: annotation301155
version: 1
top level: OmpR
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0082/annotation301155/range301155/1

https://synbiohub.org/public/igem/BBa_R0082/annotation301155/range301155/1
type: Range
persistent identity: range301155
display id: range301155
version: 1
direction: direction/forward
top level: OmpR
start: 34
end: 51
orientation: inline

C3 OmpR
type: SequenceAnnotation
persistent identity: annotation301156
display id: annotation301156
version: 1
top level: OmpR
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0082/annotation301156/range301156/1

https://synbiohub.org/public/igem/BBa_R0082/annotation301156/range301156/1
type: Range
persistent identity: range301156
display id: range301156
version: 1
direction: direction/forward
top level: OmpR
start: 54
end: 71
orientation: inline

BBa_J05215
type: ComponentDefinition
persistent identity: BBa J05215
display id: BBa_J05215
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Regulator for R1-CREBH
creator: Alexander Roth
created: 2005-09-07T11:00:00Z
modified: 2015-08-31T04:08:15Z
discontinued: false
dominant: false
group u list: _34_
m user id: 0
owner id: 412
owning group id: 34
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: binding sites for R3-ATF6 and R2-YAP7
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J05215_sequence/1

hGH pA
type: ComponentDefinition
persistent identity: BBa K678018
display id: BBa_K678018
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: hGH poly A, terminator for mammalian cells
creator: DTU-Denmark-2
created: 2011-09-03T11:00:00Z
modified: 2015-05-08T01:13:04Z
discontinued: false
dominant: false
experience: experience/None
group u list: _882_
m user id: 0
owner id: 8341
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: hGH poly A, terminator for mammalian cells
mutable notes: Compatible with the Plug'n'Play assembly standard
mutable provenance: From plasmid N-terminal pFLAG-CMV from Sigma-Aldrich
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: hGH
sequence annotation: Linker 4
sequence: https://synbiohub.org/public/igem/BBa_K678018_sequence/1

hGH
type: SequenceAnnotation
persistent identity: annotation2127351
display id: annotation2127351
version: 1
top level: hGH pA
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_K678018/annotation2127351/range2127351/1

https://synbiohub.org/public/igem/BBa_K678018/annotation2127351/range2127351/1
type: Range
persistent identity: range2127351
display id: range2127351
version: 1
direction: direction/forward
top level: hGH pA
start: 9
end: 635
orientation: inline

Linker 4
type: SequenceAnnotation
persistent identity: annotation2127350
display id: annotation2127350
version: 1
top level: hGH pA
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K678018/annotation2127350/range2127350/1

https://synbiohub.org/public/igem/BBa_K678018/annotation2127350/range2127350/1
type: Range
persistent identity: range2127350
display id: range2127350
version: 1
direction: direction/forward
top level: hGH pA
start: 1
end: 8
orientation: inline

U061O11+D0
type: ComponentDefinition
persistent identity: BBa I732435
display id: BBa_I732435
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Family Member (U061O11+D050O22)
creator: Zhan Jian
created: 2007-10-13T11:00:00Z
modified: 2015-08-31T04:07:58Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732435_sequence/1

U097O11+D0
type: ComponentDefinition
persistent identity: BBa I732401
display id: BBa_I732401
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Family Member (U097O11+D062NUL)
creator: Zhan Jian
created: 2007-10-02T11:00:00Z
modified: 2015-08-31T04:07:58Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1592
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Our first NAND logical gate
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732401_sequence/1

BBa_J61104
type: ComponentDefinition
persistent identity: BBa J61104
display id: BBa_J61104
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Ribosome Binding Site Family Member
creator: John Anderson
created: 2007-04-23T11:00:00Z
modified: 2015-08-31T02:03:00Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: false
mutable description: {{JCA_Arkin_RBSFamily}}
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J61104_sequence/1

BBa_K105029
type: ComponentDefinition
persistent identity: BBa K105029
display id: BBa_K105029
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: cyc43 minimal promoter
creator: Michael Wild
created: 2008-10-25T11:00:00Z
modified: 2015-05-08T01:08:52Z
discontinued: false
dominant: false
experience: experience/None
group u list: _253_
m user id: 0
owner id: 3313
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This is a derivate of BBa_K105027. Due to a point mutation in the TATA box region the efficiency of this minimal promoter is only 43 % of the natural yeast CYC1 promoter.
mutable notes: This BioBrick contains the TATA-box with one point mutation and most of the 5'UTR (-139 to -36, relative to the translational start) of the natural CYC1 promoter. A Kozak sequence is not included and has thus to be added! Even if there isn't any need for this BioBrick to be fusible to other protein domains, we stayed with our design.
mutable provenance: Amplification and Mutation from the plasmid pAH3
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: TATA-box
sequence annotation: A -> G
sequence: https://synbiohub.org/public/igem/BBa_K105029_sequence/1

TATA-box
type: SequenceAnnotation
persistent identity: annotation1989837
display id: annotation1989837
version: 1
top level: BBa_K105029
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K105029/annotation1989837/range1989837/1

https://synbiohub.org/public/igem/BBa_K105029/annotation1989837/range1989837/1
type: Range
persistent identity: range1989837
display id: range1989837
version: 1
direction: direction/forward
top level: BBa_K105029
start: 17
end: 23
orientation: inline

A -> G
type: SequenceAnnotation
persistent identity: annotation1989838
display id: annotation1989838
version: 1
top level: BBa_K105029
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_K105029/annotation1989838/range1989838/1

https://synbiohub.org/public/igem/BBa_K105029/annotation1989838/range1989838/1
type: Range
persistent identity: range1989838
display id: range1989838
version: 1
direction: direction/forward
top level: BBa_K105029
start: 23
end: 23
orientation: inline

GAL1
type: ComponentDefinition
persistent identity: BBa J63006
display id: BBa_J63006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: yeast GAL1 promoter
creator: Caroline Ajo-Franklin
created: 2006-10-10T11:00:00Z
modified: 2015-08-31T01:56:26Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _97_
m user id: 0
owner id: 545
owning group id: 97
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: GAL1 promoter from S. cerevisiae
mutable notes: unchanged from genomic sequence
mutable provenance: genomic GAL1 locus
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J63006_sequence/1

BBa_K1897002
type: ComponentDefinition
persistent identity: BBa K1897002
display id: BBa_K1897002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: HasR Membrane receptor coding sequence
creator: Choi Yan Ru
created: 2016-10-08T11:00:00Z
modified: 2016-10-09T02:50:37Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2363_
m user id: 29428
owner id: 29428
owning group id: 9
status: status/Planning
bookmark: false
mutable description: HasR is the membrane-bound receptor in the Has system, a heme acquisition system originally from the Serratia marcescens. The HasR protein is involved in binding with a heme-loaded HasA hemophore to trigger downstream HasS and HasI. It has been shown that the N terminal of HasR is especially crucial for signal transduction. With the heme-loaded hasA bound to hasR, it induces a conformational change in HasR that causes the activation of HasS, an anti-sigma factor. HasS then releases HasI (sigma factor) that was initially bound to it and HasI ultimately is able to trigger expression of genes under control of pHas (part number).
mutable notes: NIL
mutable provenance: The HasR gene is originally found in the Serratia marcescens. We obtained the gene sequence from the National Center for Biotechnology Information (NCBI) database (http://www.ncbi.nlm.nih.gov/nuccore/X81195.2) and synthesised the gene.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Stop codon
sequence annotation: Start codon
sequence annotation: HasR
sequence: https://synbiohub.org/public/igem/BBa_K1897002_sequence/1

Stop codon
type: SequenceAnnotation
persistent identity: annotation2489471
display id: annotation2489471
version: 1
top level: BBa_K1897002
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1897002/annotation2489471/range2489471/1

https://synbiohub.org/public/igem/BBa_K1897002/annotation2489471/range2489471/1
type: Range
persistent identity: range2489471
display id: range2489471
version: 1
direction: direction/reverse
top level: BBa_K1897002
start: 2698
end: 2700
orientation: inline

Start codon
type: SequenceAnnotation
persistent identity: annotation2489469
display id: annotation2489469
version: 1
top level: BBa_K1897002
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1897002/annotation2489469/range2489469/1

https://synbiohub.org/public/igem/BBa_K1897002/annotation2489469/range2489469/1
type: Range
persistent identity: range2489469
display id: range2489469
version: 1
direction: direction/forward
top level: BBa_K1897002
start: 1
end: 3
orientation: inline

HasR
type: SequenceAnnotation
persistent identity: annotation2489470
display id: annotation2489470
version: 1
top level: BBa_K1897002
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1897002/annotation2489470/range2489470/1

https://synbiohub.org/public/igem/BBa_K1897002/annotation2489470/range2489470/1
type: Range
persistent identity: range2489470
display id: range2489470
version: 1
direction: direction/forward
top level: BBa_K1897002
start: 1
end: 2700
orientation: inline

U049O11+D0
type: ComponentDefinition
persistent identity: BBa I732439
display id: BBa_I732439
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Family Member (U049O11+D026O22)
creator: Zhan Jian
created: 2007-10-13T11:00:00Z
modified: 2015-08-31T04:07:58Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732439_sequence/1

BBa_K2042006
type: ComponentDefinition
persistent identity: BBa K2042006
display id: BBa_K2042006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Cyclohexanone inducible promoter
creator: Robert Ram??rez Garc??a
created: 2016-09-01T11:00:00Z
modified: 2016-10-29T06:01:50Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2509_
m user id: 31380
owner id: 32514
owning group id: 9
status: status/Planning
bookmark: false
mutable description: A
mutable notes: A
mutable provenance: A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: chnB promoter region
sequence: https://synbiohub.org/public/igem/BBa_K2042006_sequence/1

chnB promoter region
type: SequenceAnnotation
persistent identity: annotation2489032
display id: annotation2489032
version: 1
top level: BBa_K2042006
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K2042006/annotation2489032/range2489032/1

https://synbiohub.org/public/igem/BBa_K2042006/annotation2489032/range2489032/1
type: Range
persistent identity: range2489032
display id: range2489032
version: 1
direction: direction/forward
top level: BBa_K2042006
start: 11
end: 406
orientation: inline

BBa_B2102
type: ComponentDefinition
persistent identity: BBa B2102
display id: BBa_B2102
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: T7 RBS 0.4 + SapI (rev)
creator: Heather Keller
created: 2006-09-27T11:00:00Z
modified: 2015-08-31T04:07:21Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_
m user id: 0
owner id: 571
owning group id: 10
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Insert Text here
mutable notes: Insert Text here
mutable provenance: Insert Text here
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: BBa_B0079
sequence annotation: BBa_B2002
sequence annotation: SD region
sequence annotation: SapI
sequence: https://synbiohub.org/public/igem/BBa_B2102_sequence/1

BBa_B0079
type: SequenceAnnotation
persistent identity: annotation1902501
display id: annotation1902501
version: 1
top level: BBa_B2102
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_B2102/annotation1902501/range1902501/1

https://synbiohub.org/public/igem/BBa_B2102/annotation1902501/range1902501/1
type: Range
persistent identity: range1902501
display id: range1902501
version: 1
direction: direction/forward
top level: BBa_B2102
start: 21
end: 32
orientation: inline

BBa_B2002
type: SequenceAnnotation
persistent identity: annotation1902499
display id: annotation1902499
version: 1
top level: BBa_B2102
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_B2102/annotation1902499/range1902499/1

https://synbiohub.org/public/igem/BBa_B2102/annotation1902499/range1902499/1
type: Range
persistent identity: range1902499
display id: range1902499
version: 1
direction: direction/forward
top level: BBa_B2102
start: 1
end: 20
orientation: inline

SD region
type: SequenceAnnotation
persistent identity: annotation1902500
display id: annotation1902500
version: 1
top level: BBa_B2102
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_B2102/annotation1902500/range1902500/1

https://synbiohub.org/public/igem/BBa_B2102/annotation1902500/range1902500/1
type: Range
persistent identity: range1902500
display id: range1902500
version: 1
direction: direction/forward
top level: BBa_B2102
start: 4
end: 10
orientation: inline

SapI
type: SequenceAnnotation
persistent identity: annotation1902502
display id: annotation1902502
version: 1
top level: BBa_B2102
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_B2102/annotation1902502/range1902502/1

https://synbiohub.org/public/igem/BBa_B2102/annotation1902502/range1902502/1
type: Range
persistent identity: range1902502
display id: range1902502
version: 1
direction: direction/reverse
top level: BBa_B2102
start: 25
end: 31
orientation: inline

BBa_J64001
type: ComponentDefinition
persistent identity: BBa J64001
display id: BBa_J64001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: psicA from Salmonella
creator: Dan Widmaier
created: 2008-01-14T12:00:00Z
modified: 2015-08-31T01:56:26Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _98_
m user id: 0
owner id: 2407
owning group id: 98
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: The sicA promoter is a regulatory element in the Salmonella SPI-1 secretion system. It controls the expression of secreted effector proteins in the natural system and turns on late. Please reference Temme et al, 2008, JMB for exact dynamics of this promoter and associated feedback mechanisms. This promoter only functions in Salmonella strains containing SPI-1.
mutable notes: This part can only be used in salmonella strains.
mutable provenance: Salmonella LT2 Genomic DNA
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: sicA Promoter
sequence annotation: +1 Transcriptional Start Site
sequence annotation: InvF:SicA Binding Box
sequence annotation: -10 Hexamer
sequence annotation: -35 Hexamer
sequence: https://synbiohub.org/public/igem/BBa_J64001_sequence/1

sicA Promoter
type: SequenceAnnotation
persistent identity: annotation1959627
display id: annotation1959627
version: 1
top level: BBa_J64001
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_J64001/annotation1959627/range1959627/1

https://synbiohub.org/public/igem/BBa_J64001/annotation1959627/range1959627/1
type: Range
persistent identity: range1959627
display id: range1959627
version: 1
direction: direction/forward
top level: BBa_J64001
start: 1
end: 143
orientation: inline

+1 Transcriptional Start Site
type: SequenceAnnotation
persistent identity: annotation1959631
display id: annotation1959631
version: 1
top level: BBa_J64001
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_J64001/annotation1959631/range1959631/1

https://synbiohub.org/public/igem/BBa_J64001/annotation1959631/range1959631/1
type: Range
persistent identity: range1959631
display id: range1959631
version: 1
direction: direction/forward
top level: BBa_J64001
start: 106
end: 106
orientation: inline

InvF:SicA Binding Box
type: SequenceAnnotation
persistent identity: annotation1959628
display id: annotation1959628
version: 1
top level: BBa_J64001
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_J64001/annotation1959628/range1959628/1

https://synbiohub.org/public/igem/BBa_J64001/annotation1959628/range1959628/1
type: Range
persistent identity: range1959628
display id: range1959628
version: 1
direction: direction/forward
top level: BBa_J64001
start: 54
end: 65
orientation: inline

-10 Hexamer
type: SequenceAnnotation
persistent identity: annotation1959630
display id: annotation1959630
version: 1
top level: BBa_J64001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J64001/annotation1959630/range1959630/1

https://synbiohub.org/public/igem/BBa_J64001/annotation1959630/range1959630/1
type: Range
persistent identity: range1959630
display id: range1959630
version: 1
direction: direction/forward
top level: BBa_J64001
start: 94
end: 100
orientation: inline

-35 Hexamer
type: SequenceAnnotation
persistent identity: annotation1959629
display id: annotation1959629
version: 1
top level: BBa_J64001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J64001/annotation1959629/range1959629/1

https://synbiohub.org/public/igem/BBa_J64001/annotation1959629/range1959629/1
type: Range
persistent identity: range1959629
display id: range1959629
version: 1
direction: direction/forward
top level: BBa_J64001
start: 71
end: 77
orientation: inline

U097O11+D0
type: ComponentDefinition
persistent identity: BBa I732415
display id: BBa_I732415
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Family Member (U097O11+D026O22)
creator: Zhan Jian
created: 2007-10-13T11:00:00Z
modified: 2015-08-31T04:07:58Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732415_sequence/1

BBa_K086019
type: ComponentDefinition
persistent identity: BBa K086019
display id: BBa_K086019
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: modified Lutz-Bujard LacO promoter,with alternative sigma factor σ24
creator: Gairik Sachdeva
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:08:36Z
discontinued: false
dominant: false
group u list: _197_
m user id: 0
owner id: 2578
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: modified Lutz-Bujard LacO promoter,with alternative sigma factor σ24
mutable notes: Cleaned
mutable provenance: Lac O and sigma 24 binding conserved sequences
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: P24_2
sequence: https://synbiohub.org/public/igem/BBa_K086019_sequence/1

P24_2
type: SequenceAnnotation
persistent identity: annotation1995753
display id: annotation1995753
version: 1
top level: BBa_K086019
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K086019/annotation1995753/range1995753/1

https://synbiohub.org/public/igem/BBa_K086019/annotation1995753/range1995753/1
type: Range
persistent identity: range1995753
display id: range1995753
version: 1
direction: direction/forward
top level: BBa_K086019
start: 1
end: 55
orientation: inline

BBa_K2003010
type: ComponentDefinition
persistent identity: BBa K2003010
display id: BBa_K2003010
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Inducible Green Fluorescent Protein UnaG+6xHis-tag
creator: Philip L??fgren
created: 2016-08-31T11:00:00Z
modified: 2016-10-16T06:08:47Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2470_
m user id: 31672
owner id: 31672
owning group id: 9
status: status/Planning
bookmark: false
mutable description: WiP
mutable notes: Potato
mutable provenance: Eel
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: UnaG
sequence annotation: Untranscribed flexible linker
sequence annotation: Hexahistidine tag
sequence annotation: 2x Stop codons
sequence annotation: Start
sequence: https://synbiohub.org/public/igem/BBa_K2003010_sequence/1

UnaG
type: SequenceAnnotation
persistent identity: annotation2512839
display id: annotation2512839
version: 1
top level: BBa_K2003010
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K2003010/annotation2512839/range2512839/1

https://synbiohub.org/public/igem/BBa_K2003010/annotation2512839/range2512839/1
type: Range
persistent identity: range2512839
display id: range2512839
version: 1
direction: direction/forward
top level: BBa_K2003010
start: 19
end: 439
orientation: inline

Untranscribed flexible linker
type: SequenceAnnotation
persistent identity: annotation2512841
display id: annotation2512841
version: 1
top level: BBa_K2003010
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K2003010/annotation2512841/range2512841/1

https://synbiohub.org/public/igem/BBa_K2003010/annotation2512841/range2512841/1
type: Range
persistent identity: range2512841
display id: range2512841
version: 1
direction: direction/forward
top level: BBa_K2003010
start: 445
end: 462
orientation: inline

Hexahistidine tag
type: SequenceAnnotation
persistent identity: annotation2512838
display id: annotation2512838
version: 1
top level: BBa_K2003010
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K2003010/annotation2512838/range2512838/1

https://synbiohub.org/public/igem/BBa_K2003010/annotation2512838/range2512838/1
type: Range
persistent identity: range2512838
display id: range2512838
version: 1
direction: direction/forward
top level: BBa_K2003010
start: 4
end: 18
orientation: inline

2x Stop codons
type: SequenceAnnotation
persistent identity: annotation2512840
display id: annotation2512840
version: 1
top level: BBa_K2003010
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K2003010/annotation2512840/range2512840/1

https://synbiohub.org/public/igem/BBa_K2003010/annotation2512840/range2512840/1
type: Range
persistent identity: range2512840
display id: range2512840
version: 1
direction: direction/forward
top level: BBa_K2003010
start: 439
end: 444
orientation: inline

Start
type: SequenceAnnotation
persistent identity: annotation2512842
display id: annotation2512842
version: 1
top level: BBa_K2003010
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K2003010/annotation2512842/range2512842/1

https://synbiohub.org/public/igem/BBa_K2003010/annotation2512842/range2512842/1
type: Range
persistent identity: range2512842
display id: range2512842
version: 1
direction: direction/forward
top level: BBa_K2003010
start: 1
end: 3
orientation: inline

BBa_K145152
type: ComponentDefinition
persistent identity: BBa K145152
display id: BBa_K145152
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Hybrid promoter: P22 c2 , LacI NOR gate
creator: Jonas Demeulemeester
created: 2008-08-11T11:00:00Z
modified: 2015-05-08T01:10:29Z
discontinued: false
dominant: false
group u list: _257_
m user id: 0
owner id: 2970
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Hybrid promoter constisting of two LacI O2 sites at the ends of the promoter and two recognition sites (OR2 and OR1) for the P22 c2 repressor. The promoter should function as a NOR gate.
mutable notes: a small fragment of OR3 remains.
mutable provenance: synthetic
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: O2 P22 c2
sequence annotation: O1 P22 c2
sequence annotation: O2 LacI
sequence annotation: -10
sequence annotation: -35
sequence: https://synbiohub.org/public/igem/BBa_K145152_sequence/1

O2 P22 c2
type: SequenceAnnotation
persistent identity: annotation1971581
display id: annotation1971581
version: 1
top level: BBa_K145152
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K145152/annotation1971581/range1971581/1

https://synbiohub.org/public/igem/BBa_K145152/annotation1971581/range1971581/1
type: Range
persistent identity: range1971581
display id: range1971581
version: 1
direction: direction/forward
top level: BBa_K145152
start: 59
end: 76
orientation: inline

O1 P22 c2
type: SequenceAnnotation
persistent identity: annotation1971587
display id: annotation1971587
version: 1
top level: BBa_K145152
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K145152/annotation1971587/range1971587/1

https://synbiohub.org/public/igem/BBa_K145152/annotation1971587/range1971587/1
type: Range
persistent identity: range1971587
display id: range1971587
version: 1
direction: direction/forward
top level: BBa_K145152
start: 82
end: 97
orientation: inline

O2 LacI
type: SequenceAnnotation
persistent identity: annotation1971579
display id: annotation1971579
version: 1
top level: BBa_K145152
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K145152/annotation1971579/range1971579/1

https://synbiohub.org/public/igem/BBa_K145152/annotation1971579/range1971579/1
type: Range
persistent identity: range1971579
display id: range1971579
version: 1
direction: direction/forward
top level: BBa_K145152
start: 1
end: 21
orientation: inline

O2 LacI
type: SequenceAnnotation
persistent identity: annotation1971580
display id: annotation1971580
version: 1
top level: BBa_K145152
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K145152/annotation1971580/range1971580/1

https://synbiohub.org/public/igem/BBa_K145152/annotation1971580/range1971580/1
type: Range
persistent identity: range1971580
display id: range1971580
version: 1
direction: direction/forward
top level: BBa_K145152
start: 115
end: 135
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1971558
display id: annotation1971558
version: 1
top level: BBa_K145152
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K145152/annotation1971558/range1971558/1

https://synbiohub.org/public/igem/BBa_K145152/annotation1971558/range1971558/1
type: Range
persistent identity: range1971558
display id: range1971558
version: 1
direction: direction/forward
top level: BBa_K145152
start: 78
end: 83
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1971557
display id: annotation1971557
version: 1
top level: BBa_K145152
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K145152/annotation1971557/range1971557/1

https://synbiohub.org/public/igem/BBa_K145152/annotation1971557/range1971557/1
type: Range
persistent identity: range1971557
display id: range1971557
version: 1
direction: direction/forward
top level: BBa_K145152
start: 56
end: 61
orientation: inline

pJEJAM1
type: ComponentDefinition
persistent identity: BBa K678049
display id: BBa_K678049
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pJEJAM1
creator: DTU-Denmark-2
created: 2011-09-12T11:00:00Z
modified: 2015-05-08T01:13:04Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _882_
m user id: 0
owner id: 8341
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: to be announced
mutable notes: to be announced
mutable provenance: to be announced
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000155
role: partType/Plasmid
component: BBa_K678023
component: BBa_J52034
component: BBa_K678006
component: BBa_K678019
component: BBa_K678021
sequence annotation: BBa_K678019
sequence annotation: BBa_K678021
sequence annotation: BBa_J52034
sequence annotation: BBa_K678023
sequence annotation: BBa_K678006
sequence: https://synbiohub.org/public/igem/BBa_K678049_sequence/1

BBa_K678023
type: Component
persistent identity: component2135861
display id: component2135861
version: 1
top level: pJEJAM1
access: public
definition: pMam

BBa_J52034
type: Component
persistent identity: component2135848
display id: component2135848
version: 1
top level: pJEJAM1
access: public
definition: https://synbiohub.org/public/igem/BBa_J52034/1

BBa_K678006
type: Component
persistent identity: component2135852
display id: component2135852
version: 1
top level: pJEJAM1
access: public
definition: eGFP

BBa_K678019
type: Component
persistent identity: component2135855
display id: component2135855
version: 1
top level: pJEJAM1
access: public
definition: BGH pA

BBa_K678021
type: Component
persistent identity: component2135858
display id: component2135858
version: 1
top level: pJEJAM1
access: public
definition: hygR

BBa_K678019
type: SequenceAnnotation
persistent identity: annotation2135855
display id: annotation2135855
version: 1
top level: pJEJAM1
location: https://synbiohub.org/public/igem/BBa_K678049/annotation2135855/range2135855/1
component: BBa_K678019

https://synbiohub.org/public/igem/BBa_K678049/annotation2135855/range2135855/1
type: Range
persistent identity: range2135855
display id: range2135855
version: 1
direction: direction/reverse
top level: pJEJAM1
start: 1390
end: 1622
orientation: inline

BBa_K678021
type: SequenceAnnotation
persistent identity: annotation2135858
display id: annotation2135858
version: 1
top level: pJEJAM1
location: https://synbiohub.org/public/igem/BBa_K678049/annotation2135858/range2135858/1
component: BBa_K678021

https://synbiohub.org/public/igem/BBa_K678049/annotation2135858/range2135858/1
type: Range
persistent identity: range2135858
display id: range2135858
version: 1
direction: direction/reverse
top level: pJEJAM1
start: 1623
end: 3381
orientation: inline

BBa_J52034
type: SequenceAnnotation
persistent identity: annotation2135848
display id: annotation2135848
version: 1
top level: pJEJAM1
location: https://synbiohub.org/public/igem/BBa_K678049/annotation2135848/range2135848/1
component: BBa_J52034

https://synbiohub.org/public/igem/BBa_K678049/annotation2135848/range2135848/1
type: Range
persistent identity: range2135848
display id: range2135848
version: 1
direction: direction/reverse
top level: pJEJAM1
start: 1
end: 654
orientation: inline

BBa_K678023
type: SequenceAnnotation
persistent identity: annotation2135861
display id: annotation2135861
version: 1
top level: pJEJAM1
location: https://synbiohub.org/public/igem/BBa_K678049/annotation2135861/range2135861/1
component: BBa_K678023

https://synbiohub.org/public/igem/BBa_K678049/annotation2135861/range2135861/1
type: Range
persistent identity: range2135861
display id: range2135861
version: 1
direction: direction/reverse
top level: pJEJAM1
start: 3382
end: 6092
orientation: inline

BBa_K678006
type: SequenceAnnotation
persistent identity: annotation2135852
display id: annotation2135852
version: 1
top level: pJEJAM1
location: https://synbiohub.org/public/igem/BBa_K678049/annotation2135852/range2135852/1
component: BBa_K678006

https://synbiohub.org/public/igem/BBa_K678049/annotation2135852/range2135852/1
type: Range
persistent identity: range2135852
display id: range2135852
version: 1
direction: direction/reverse
top level: pJEJAM1
start: 655
end: 1389
orientation: inline

U037O11+D0
type: ComponentDefinition
persistent identity: BBa I732446
display id: BBa_I732446
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Family Member (U037O11+D038O22)
creator: Zhan Jian
created: 2007-10-13T11:00:00Z
modified: 2015-08-31T04:07:58Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732446_sequence/1

BBa_K1486029
type: ComponentDefinition
persistent identity: BBa K1486029
display id: BBa_K1486029
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Yeast Optimized sfGFP-N + ADH1 terminator + Kanamycin resistance
creator: EPFL iGem team 2014
created: 2014-09-26T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: To edit
mutable notes: To edit
mutable provenance: To edit
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K1486028
component: BBa_K1486024
component: BBa_K1486037
component: BBa_K1486025
sequence annotation: BBa_K1486028
sequence annotation: BBa_K1486025
sequence annotation: BBa_K1486024
sequence annotation: BBa_K1486037
sequence: https://synbiohub.org/public/igem/BBa_K1486029_sequence/1

BBa_K1486028
type: Component
persistent identity: component2417699
display id: component2417699
version: 1
top level: BBa_K1486029
access: public
definition: BBa_K1486028

BBa_K1486024
type: Component
persistent identity: component2417704
display id: component2417704
version: 1
top level: BBa_K1486029
access: public
definition: BBa_K1486024

BBa_K1486037
type: Component
persistent identity: component2417697
display id: component2417697
version: 1
top level: BBa_K1486029
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486037/1

BBa_K1486025
type: Component
persistent identity: component2417700
display id: component2417700
version: 1
top level: BBa_K1486029
access: public
definition: BBa_K1486025

BBa_K1486028
type: SequenceAnnotation
persistent identity: annotation2417699
display id: annotation2417699
version: 1
top level: BBa_K1486029
location: https://synbiohub.org/public/igem/BBa_K1486029/annotation2417699/range2417699/1
component: BBa_K1486028

https://synbiohub.org/public/igem/BBa_K1486029/annotation2417699/range2417699/1
type: Range
persistent identity: range2417699
display id: range2417699
version: 1
direction: direction/reverse
top level: BBa_K1486029
start: 40
end: 681
orientation: inline

BBa_K1486025
type: SequenceAnnotation
persistent identity: annotation2417700
display id: annotation2417700
version: 1
top level: BBa_K1486029
location: https://synbiohub.org/public/igem/BBa_K1486029/annotation2417700/range2417700/1
component: BBa_K1486025

https://synbiohub.org/public/igem/BBa_K1486029/annotation2417700/range2417700/1
type: Range
persistent identity: range2417700
display id: range2417700
version: 1
direction: direction/reverse
top level: BBa_K1486029
start: 682
end: 869
orientation: inline

BBa_K1486024
type: SequenceAnnotation
persistent identity: annotation2417704
display id: annotation2417704
version: 1
top level: BBa_K1486029
location: https://synbiohub.org/public/igem/BBa_K1486029/annotation2417704/range2417704/1
component: BBa_K1486024

https://synbiohub.org/public/igem/BBa_K1486029/annotation2417704/range2417704/1
type: Range
persistent identity: range2417704
display id: range2417704
version: 1
direction: direction/reverse
top level: BBa_K1486029
start: 870
end: 2226
orientation: inline

BBa_K1486037
type: SequenceAnnotation
persistent identity: annotation2417697
display id: annotation2417697
version: 1
top level: BBa_K1486029
location: https://synbiohub.org/public/igem/BBa_K1486029/annotation2417697/range2417697/1
component: BBa_K1486037

https://synbiohub.org/public/igem/BBa_K1486029/annotation2417697/range2417697/1
type: Range
persistent identity: range2417697
display id: range2417697
version: 1
direction: direction/reverse
top level: BBa_K1486029
start: 1
end: 39
orientation: inline

BBa_K1486023
type: ComponentDefinition
persistent identity: BBa K1486023
display id: BBa_K1486023
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Yeast optimized superfolder GFP
creator: EPFL iGem team 2014
created: 2014-09-25T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: To edit
mutable notes: To edit
mutable provenance: To edit
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: Yo sfGFP
sequence: https://synbiohub.org/public/igem/BBa_K1486023_sequence/1

Yo sfGFP
type: SequenceAnnotation
persistent identity: annotation2418775
display id: annotation2418775
version: 1
top level: BBa_K1486023
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1486023/annotation2418775/range2418775/1

https://synbiohub.org/public/igem/BBa_K1486023/annotation2418775/range2418775/1
type: Range
persistent identity: range2418775
display id: range2418775
version: 1
direction: direction/forward
top level: BBa_K1486023
start: 1
end: 717
orientation: inline

BBa_R0180
type: ComponentDefinition
persistent identity: BBa R0180
display id: BBa_R0180
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: T7 RNAP promoter
creator: Barry Canton
created: 2005-09-25T11:00:00Z
modified: 2015-05-08T01:14:15Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_6_
m user id: 0
owner id: 135
owning group id: 6
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: T7 promoter with an A->T mutation at the -16 position of the consensus sequence
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_R0180_sequence/1

BBa_K259001
type: ComponentDefinition
persistent identity: BBa K259001
display id: BBa_K259001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: fiu ??? Outer Membrane Ferric Iron Transporter
creator: Petros Mina
created: 2009-07-13T11:00:00Z
modified: 2015-05-08T01:11:42Z
discontinued: false
dominant: true
group u list: _357_
m user id: 0
owner id: 5318
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: ===Biology of Fiu=== The fiu gene encodes for an outer membrane transporter found in E.coli, a gram negative bacterium. It is involved in the active transport (energy dependent uptake) of ferric iron from the extracellular environment. This outer membrane receptor can only bind ferric iron and is a catecholate siderophore. For uptake into the cell to be completed fiu has to complex with another protein known as TonB (a transperiplasmic protein) that will carry out the active transport by using ATP as an energy source. ===What this Biobrick gives you upon expression=== This biobrick encodes ONLY for fiu. ===Fiu Statistics=== Fiu belongs to the family of Ton-B dependent receptors and is related to fhuA, hence it also has a beta-barrel structure. {| style="color:white; background-color:#0066CC;" cellpadding="20" cellspacing="0" border="2" !Chassis !Monomer/Multimer !Aminoacid Length/Weight !Localisation !Structure |- |E.Coli |Monomer |760/81.96kDa |Outer Membrane/OMV |beta-barrel |} ===References=== [http://ecoliwiki.net/colipedia/index.php/fiu:Gene_Product%28s%29#Domains.2FMotifs.2FModification_Sites E.Coli wiki net] Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner) [http://www.ecogene.org/geneInfo.php?eg_id=EG13317 Ecogene number EG13317] [http://www.uniprot.org/uniprot/P75780 UniProt Accession number P75780]
mutable notes: In order to comply with assembly standard 10 (RFC10) the stop codon for fiu had to be changed to TAA.
mutable provenance: Fiu gene is part of the chromosomal E.coli genome. In particular it is found at 18.07min with orientation in the counter-clockwise direction. [http://www.ecogene.org/geneInfo.php?eg_id=EG13317 Further details at EcoGene]
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Signal Sequence
sequence annotation: stop
sequence annotation: start
sequence annotation: Multipass protein (OM-Periplasm)
sequence: https://synbiohub.org/public/igem/BBa_K259001_sequence/1

Signal Sequence
type: SequenceAnnotation
persistent identity: annotation2011315
display id: annotation2011315
version: 1
top level: BBa_K259001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K259001/annotation2011315/range2011315/1

https://synbiohub.org/public/igem/BBa_K259001/annotation2011315/range2011315/1
type: Range
persistent identity: range2011315
display id: range2011315
version: 1
direction: direction/forward
top level: BBa_K259001
start: 1
end: 99
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2011314
display id: annotation2011314
version: 1
top level: BBa_K259001
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K259001/annotation2011314/range2011314/1

https://synbiohub.org/public/igem/BBa_K259001/annotation2011314/range2011314/1
type: Range
persistent identity: range2011314
display id: range2011314
version: 1
direction: direction/forward
top level: BBa_K259001
start: 2243
end: 2247
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2011313
display id: annotation2011313
version: 1
top level: BBa_K259001
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K259001/annotation2011313/range2011313/1

https://synbiohub.org/public/igem/BBa_K259001/annotation2011313/range2011313/1
type: Range
persistent identity: range2011313
display id: range2011313
version: 1
direction: direction/forward
top level: BBa_K259001
start: 1
end: 3
orientation: inline

Multipass protein (OM-Periplasm)
type: SequenceAnnotation
persistent identity: annotation2011316
display id: annotation2011316
version: 1
top level: BBa_K259001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K259001/annotation2011316/range2011316/1

https://synbiohub.org/public/igem/BBa_K259001/annotation2011316/range2011316/1
type: Range
persistent identity: range2011316
display id: range2011316
version: 1
direction: direction/forward
top level: BBa_K259001
start: 100
end: 2247
orientation: inline

U097O11+D0
type: ComponentDefinition
persistent identity: BBa I732416
display id: BBa_I732416
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Family Member (U097O11+D038O22)
creator: Zhan Jian
created: 2007-10-13T11:00:00Z
modified: 2015-08-31T04:07:58Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732416_sequence/1

CP1LacPI2
type: ComponentDefinition
persistent identity: BBa K418002
display id: BBa_K418002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: IPTG inducible Lac promoter cassette
creator: Northwestern University iGEM 2010
created: 2010-10-15T11:00:00Z
modified: 2015-05-08T01:12:28Z
discontinued: false
dominant: false
experience: experience/None
group u list: _532_
m user id: 0
owner id: 6938
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_Q04121
component: BBa_J23100
sequence annotation: BBa_Q04121
sequence annotation: BBa_J23100
sequence: https://synbiohub.org/public/igem/BBa_K418002_sequence/1

BBa_Q04121
type: Component
persistent identity: component2263762
display id: component2263762
version: 1
top level: CP1LacPI2
access: public
definition: https://synbiohub.org/public/igem/BBa_Q04121/1

BBa_J23100
type: Component
persistent identity: component2263743
display id: component2263743
version: 1
top level: CP1LacPI2
access: public
definition: BBa_J23100

BBa_Q04121
type: SequenceAnnotation
persistent identity: annotation2263762
display id: annotation2263762
version: 1
top level: CP1LacPI2
location: https://synbiohub.org/public/igem/BBa_K418002/annotation2263762/range2263762/1
component: BBa_Q04121

https://synbiohub.org/public/igem/BBa_K418002/annotation2263762/range2263762/1
type: Range
persistent identity: range2263762
display id: range2263762
version: 1
direction: direction/reverse
top level: CP1LacPI2
start: 44
end: 1414
orientation: inline

BBa_J23100
type: SequenceAnnotation
persistent identity: annotation2263743
display id: annotation2263743
version: 1
top level: CP1LacPI2
location: https://synbiohub.org/public/igem/BBa_K418002/annotation2263743/range2263743/1
component: BBa_J23100

https://synbiohub.org/public/igem/BBa_K418002/annotation2263743/range2263743/1
type: Range
persistent identity: range2263743
display id: range2263743
version: 1
direction: direction/reverse
top level: CP1LacPI2
start: 1
end: 35
orientation: inline

pspoIIE
type: ComponentDefinition
persistent identity: BBa J01005
display id: BBa_J01005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pspoIIE promoter (spo0A J01004, positive)
creator: Golden Bear
created: 2005-10-31T12:00:00Z
modified: 2015-08-31T04:08:11Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _13_
m user id: 0
owner id: 395
owning group id: 13
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: promoter from b. subtilis, activated by spo0A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J01005_sequence/1

BBa_K137089
type: ComponentDefinition
persistent identity: BBa K137089
display id: BBa_K137089
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: optimized (TA) repeat constitutive promoter with 21 bp between -10 and -35 elements
creator: Allen Lin
created: 2008-08-20T11:00:00Z
modified: 2015-05-08T01:10:10Z
discontinued: false
dominant: false
experience: experience/None
group u list: _187_
m user id: 0
owner id: 3112
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: optimized (TA) repeat constitutive promoter with 21 bp between -10 and -35 elements
mutable notes: None
mutable provenance: PCR
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K137089_sequence/1

BBa_K598013
type: ComponentDefinition
persistent identity: BBa K598013
display id: BBa_K598013
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pBAD+theoHH+CdIntron+E0040+B0015
creator: Xiaowei YAN, Tong MU
created: 2011-09-26T11:00:00Z
modified: 2015-05-08T01:12:50Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _770_
m user id: 0
owner id: 8713
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This a GFP reporter regulated by an allosteric intron responsive to theophylline.
mutable notes: The ligand response is alter from c-di-GMP to theophylline by merging a theophylline hammerhead ribozyme to the intron.
mutable provenance: The intron derives from the genome of Cd
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
sequence annotation: RBS
sequence annotation: theo HH
sequence annotation: eGFP
sequence annotation: group I intron
sequence annotation: BBa_B0015
sequence annotation: pBAD
sequence: https://synbiohub.org/public/igem/BBa_K598013_sequence/1

RBS
type: SequenceAnnotation
persistent identity: annotation2153078
display id: annotation2153078
version: 1
top level: BBa_K598013
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K598013/annotation2153078/range2153078/1

https://synbiohub.org/public/igem/BBa_K598013/annotation2153078/range2153078/1
type: Range
persistent identity: range2153078
display id: range2153078
version: 1
direction: direction/forward
top level: BBa_K598013
start: 212
end: 216
orientation: inline

theo HH
type: SequenceAnnotation
persistent identity: annotation2153072
display id: annotation2153072
version: 1
top level: BBa_K598013
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K598013/annotation2153072/range2153072/1

https://synbiohub.org/public/igem/BBa_K598013/annotation2153072/range2153072/1
type: Range
persistent identity: range2153072
display id: range2153072
version: 1
direction: direction/forward
top level: BBa_K598013
start: 139
end: 210
orientation: inline

eGFP
type: SequenceAnnotation
persistent identity: annotation2153074
display id: annotation2153074
version: 1
top level: BBa_K598013
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K598013/annotation2153074/range2153074/1

https://synbiohub.org/public/igem/BBa_K598013/annotation2153074/range2153074/1
type: Range
persistent identity: range2153074
display id: range2153074
version: 1
direction: direction/forward
top level: BBa_K598013
start: 790
end: 1509
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2153079
display id: annotation2153079
version: 1
top level: BBa_K598013
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K598013/annotation2153079/range2153079/1

https://synbiohub.org/public/igem/BBa_K598013/annotation2153079/range2153079/1
type: Range
persistent identity: range2153079
display id: range2153079
version: 1
direction: direction/forward
top level: BBa_K598013
start: 780
end: 780
orientation: inline

group I intron
type: SequenceAnnotation
persistent identity: annotation2153073
display id: annotation2153073
version: 1
top level: BBa_K598013
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K598013/annotation2153073/range2153073/1

https://synbiohub.org/public/igem/BBa_K598013/annotation2153073/range2153073/1
type: Range
persistent identity: range2153073
display id: range2153073
version: 1
direction: direction/forward
top level: BBa_K598013
start: 211
end: 789
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2153077
display id: annotation2153077
version: 1
top level: BBa_K598013
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K598013/annotation2153077/range2153077/1

https://synbiohub.org/public/igem/BBa_K598013/annotation2153077/range2153077/1
type: Range
persistent identity: range2153077
display id: range2153077
version: 1
direction: direction/forward
top level: BBa_K598013
start: 1518
end: 1646
orientation: inline

pBAD
type: SequenceAnnotation
persistent identity: annotation2153071
display id: annotation2153071
version: 1
top level: BBa_K598013
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K598013/annotation2153071/range2153071/1

https://synbiohub.org/public/igem/BBa_K598013/annotation2153071/range2153071/1
type: Range
persistent identity: range2153071
display id: range2153071
version: 1
direction: direction/forward
top level: BBa_K598013
start: 1
end: 130
orientation: inline

BBa_K292004
type: ComponentDefinition
persistent identity: BBa K292004
display id: BBa_K292004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Double terminator + Kanamycin resistance cassette + Double ter
creator: David Charoy
created: 2009-10-18T11:00:00Z
modified: 2015-05-08T01:11:49Z
discontinued: false
dominant: false
group u list: _394_
m user id: 0
owner id: 5212
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part contains a double terminator and a functional kanamycin resistance cassette (promoter, resistance against kanamycin and a double terminator, BBa_B0014 + BBa_P1003 + BBa_B0014). It is reliable to design a recombinant vector by gene insertion in a non lytic bacteriophage as the Lambda phage. In fact it allows us to know if a bacteriophage is modified or not and if the inserted gene is expressed or not. When the virus is modified the kanamycin cassette induces a kanamycin resistance, so the bacteria become resistance, and when the virus is not modified bacteria are killed by kanamycin. We can use this part as a reporter, or as a simple resistance cassette. The double terminator allows the RNA translation arrest before the resistance cassette, so this is already created to insert a coding gene with its RBS before the resistance cassette.
mutable notes: When we design the bio-brick it was impossible to obtain a functional bio-brick by using the plasmid SB1AK3. But it's worked with a pSB1A1.
mutable provenance: Part:BBa_B0014, Designed by Reshma Shetty Group: Registry (2003-07-16) Part:BBa_P1003, Designed by Reshma Shetty Group: Knight Lab, MIT (2006-01-23)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_B0011
component: BBa_B0012
component: BBa_P1003
sequence annotation: BBa_B0011
sequence annotation: BBa_P1003
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_K292004_sequence/1

BBa_B0011
type: Component
persistent identity: component2054572
display id: component2054572
version: 1
top level: BBa_K292004
access: public
definition: https://synbiohub.org/public/igem/BBa_B0011/1

BBa_B0012
type: Component
persistent identity: component2054568
display id: component2054568
version: 1
top level: BBa_K292004
access: public
definition: BBa_B0012

BBa_B0011
type: Component
persistent identity: component2054580
display id: component2054580
version: 1
top level: BBa_K292004
access: public
definition: https://synbiohub.org/public/igem/BBa_B0011/1

BBa_P1003
type: Component
persistent identity: component2054575
display id: component2054575
version: 1
top level: BBa_K292004
access: public
definition: kanR

BBa_B0012
type: Component
persistent identity: component2054576
display id: component2054576
version: 1
top level: BBa_K292004
access: public
definition: BBa_B0012

BBa_B0011
type: SequenceAnnotation
persistent identity: annotation2054572
display id: annotation2054572
version: 1
top level: BBa_K292004
location: https://synbiohub.org/public/igem/BBa_K292004/annotation2054572/range2054572/1
component: BBa_B0011

https://synbiohub.org/public/igem/BBa_K292004/annotation2054572/range2054572/1
type: Range
persistent identity: range2054572
display id: range2054572
version: 1
direction: direction/forward
top level: BBa_K292004
start: 50
end: 95
orientation: inline

BBa_B0011
type: SequenceAnnotation
persistent identity: annotation2054580
display id: annotation2054580
version: 1
top level: BBa_K292004
location: https://synbiohub.org/public/igem/BBa_K292004/annotation2054580/range2054580/1
component: BBa_B0011

https://synbiohub.org/public/igem/BBa_K292004/annotation2054580/range2054580/1
type: Range
persistent identity: range2054580
display id: range2054580
version: 1
direction: direction/forward
top level: BBa_K292004
start: 1128
end: 1173
orientation: inline

BBa_P1003
type: SequenceAnnotation
persistent identity: annotation2054575
display id: annotation2054575
version: 1
top level: BBa_K292004
location: https://synbiohub.org/public/igem/BBa_K292004/annotation2054575/range2054575/1
component: BBa_P1003

https://synbiohub.org/public/igem/BBa_K292004/annotation2054575/range2054575/1
type: Range
persistent identity: range2054575
display id: range2054575
version: 1
direction: direction/reverse
top level: BBa_K292004
start: 104
end: 1070
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2054568
display id: annotation2054568
version: 1
top level: BBa_K292004
location: https://synbiohub.org/public/igem/BBa_K292004/annotation2054568/range2054568/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K292004/annotation2054568/range2054568/1
type: Range
persistent identity: range2054568
display id: range2054568
version: 1
direction: direction/forward
top level: BBa_K292004
start: 1
end: 41
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2054576
display id: annotation2054576
version: 1
top level: BBa_K292004
location: https://synbiohub.org/public/igem/BBa_K292004/annotation2054576/range2054576/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K292004/annotation2054576/range2054576/1
type: Range
persistent identity: range2054576
display id: range2054576
version: 1
direction: direction/forward
top level: BBa_K292004
start: 1079
end: 1119
orientation: inline

BBa_R0187
type: ComponentDefinition
persistent identity: BBa R0187
display id: BBa_R0187
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: T7 promoter (lacI repressible)
creator: Bartholomew Canton
created: 2008-04-07T11:00:00Z
modified: 2015-05-08T01:14:15Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_
m user id: 0
owner id: 135
owning group id: 84
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: A lac repressible T7 promoter. This promoter is similar to those found in the pET vectors with T7lac promoters. I mutated the -9 base to an G, which should make it less than 3% of the strength of consensus. The base numbering is as used by Studier and coworkers (JMB 1991, 219, 45-59). See BBa_R0183 for more information.
mutable notes: The -9 mutation from consensus was included to weaken the consensus promoter as described by Imburgio and co-workers (see BBa_R0183 for more information)
mutable provenance: This sequence is based on the T7lac promoter described by Dubendorff and Studier (JMB 1991, 219, 45-59). The only modification is the mutation at -9.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: LacI binding site
sequence annotation: T7 promoter
sequence: https://synbiohub.org/public/igem/BBa_R0187_sequence/1

LacI binding site
type: SequenceAnnotation
persistent identity: annotation1962096
display id: annotation1962096
version: 1
top level: BBa_R0187
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0187/annotation1962096/range1962096/1

https://synbiohub.org/public/igem/BBa_R0187/annotation1962096/range1962096/1
type: Range
persistent identity: range1962096
display id: range1962096
version: 1
direction: direction/forward
top level: BBa_R0187
start: 20
end: 44
orientation: inline

T7 promoter
type: SequenceAnnotation
persistent identity: annotation1962097
display id: annotation1962097
version: 1
top level: BBa_R0187
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0187/annotation1962097/range1962097/1

https://synbiohub.org/public/igem/BBa_R0187/annotation1962097/range1962097/1
type: Range
persistent identity: range1962097
display id: range1962097
version: 1
direction: direction/forward
top level: BBa_R0187
start: 1
end: 20
orientation: inline

BBa_B0070
type: ComponentDefinition
persistent identity: BBa B0070
display id: BBa_B0070
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Specialized RBS modified from that of B0036 (Brink et al.)
creator: Barry Canton
created: 2006-03-03T12:00:00Z
modified: 2015-08-31T04:07:21Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_6_
m user id: 0
owner id: 135
owning group id: 6
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This RBS has the modified Shine-Dalgarno sequence, it has a reduced secondary structure relative to using the actual Brink sequence with mixed connective sites flanking it. the spacing between the SD and the ATG is the same as that used by Brink et al. even though it is non-optimal for E. coli
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_B0070_sequence/1

BBa_K678047
type: ComponentDefinition
persistent identity: BBa K678047
display id: BBa_K678047
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Ampicillin cassette
creator: DTU-Denmark-2
created: 2011-09-06T11:00:00Z
modified: 2015-05-08T01:13:04Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _882_
m user id: 0
owner id: 8341
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: For selection of E. coli transformants, contains promoter, ampR gene, and terminator
mutable notes: Compatible with the Plug'n'Play assembly standard
mutable provenance: From plasmid pcDNA3.1 from Invitrogen
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
sequence annotation: ampR cassette
sequence annotation: Linker 5
sequence: https://synbiohub.org/public/igem/BBa_K678047_sequence/1

ampR cassette
type: SequenceAnnotation
persistent identity: annotation2127426
display id: annotation2127426
version: 1
top level: BBa_K678047
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K678047/annotation2127426/range2127426/1

https://synbiohub.org/public/igem/BBa_K678047/annotation2127426/range2127426/1
type: Range
persistent identity: range2127426
display id: range2127426
version: 1
direction: direction/forward
top level: BBa_K678047
start: 9
end: 867
orientation: inline

Linker 5
type: SequenceAnnotation
persistent identity: annotation2127425
display id: annotation2127425
version: 1
top level: BBa_K678047
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K678047/annotation2127425/range2127425/1

https://synbiohub.org/public/igem/BBa_K678047/annotation2127425/range2127425/1
type: Range
persistent identity: range2127425
display id: range2127425
version: 1
direction: direction/forward
top level: BBa_K678047
start: 1
end: 8
orientation: inline

BBa_K266000
type: ComponentDefinition
persistent identity: BBa K266000
display id: BBa_K266000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PAI+LasR -> LuxI (AI)
creator: UNAM FCIENCIAS-CINVESTAV IRA
created: 2009-10-12T11:00:00Z
modified: 2015-05-08T01:11:43Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _363_
m user id: 0
owner id: 1955
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This biobrick has a promoter inducible by PAI+LasR (BBa_R0079)and produces LuxI enzyme (BBa_F1610). This enzyme produces 3OC6HSL (AI).
mutable notes: None
mutable provenance: The DNA of BBa_R0079 and BBa_F1610 biobricks where used.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Signalling
role: SO:0000804
component: BBa_F1610
component: BBa_R0079
sequence annotation: BBa_F1610
sequence annotation: BBa_R0079
sequence: https://synbiohub.org/public/igem/BBa_K266000_sequence/1

BBa_F1610
type: Component
persistent identity: component2256062
display id: component2256062
version: 1
top level: BBa_K266000
access: public
definition: https://synbiohub.org/public/igem/BBa_F1610/1

BBa_R0079
type: Component
persistent identity: component2256049
display id: component2256049
version: 1
top level: BBa_K266000
access: public
definition: LasR+PAI

BBa_F1610
type: SequenceAnnotation
persistent identity: annotation2256062
display id: annotation2256062
version: 1
top level: BBa_K266000
location: https://synbiohub.org/public/igem/BBa_K266000/annotation2256062/range2256062/1
component: BBa_F1610

https://synbiohub.org/public/igem/BBa_K266000/annotation2256062/range2256062/1
type: Range
persistent identity: range2256062
display id: range2256062
version: 1
direction: direction/reverse
top level: BBa_K266000
start: 166
end: 963
orientation: inline

BBa_R0079
type: SequenceAnnotation
persistent identity: annotation2256049
display id: annotation2256049
version: 1
top level: BBa_K266000
location: https://synbiohub.org/public/igem/BBa_K266000/annotation2256049/range2256049/1
component: BBa_R0079

https://synbiohub.org/public/igem/BBa_K266000/annotation2256049/range2256049/1
type: Range
persistent identity: range2256049
display id: range2256049
version: 1
direction: direction/reverse
top level: BBa_K266000
start: 1
end: 157
orientation: inline

BBa_J59001
type: ComponentDefinition
persistent identity: BBa J59001
display id: BBa_J59001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: tuba
creator: Fatos tuba
created: 2006-08-28T11:00:00Z
modified: 2015-08-31T02:02:59Z
discontinued: false
dominant: false
experience: experience/None
group u list: _93_
m user id: 0
owner id: 1162
owning group id: 93
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: none
mutable notes: none
mutable provenance: none
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: tuba
sequence: https://synbiohub.org/public/igem/BBa_J59001_sequence/1

tuba
type: SequenceAnnotation
persistent identity: annotation1898348
display id: annotation1898348
version: 1
top level: BBa_J59001
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_J59001/annotation1898348/range1898348/1

https://synbiohub.org/public/igem/BBa_J59001/annotation1898348/range1898348/1
type: Range
persistent identity: range1898348
display id: range1898348
version: 1
direction: direction/reverse
top level: BBa_J59001
start: 1
end: 25
orientation: inline

BBa_K090505
type: ComponentDefinition
persistent identity: BBa K090505
display id: BBa_K090505
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: ''Bacillus subtilis'' consensus RBS
creator: Daniel Goodman
created: 2008-10-27T12:00:00Z
modified: 2015-05-08T01:08:37Z
discontinued: false
dominant: false
experience: experience/None
group u list: _188_
m user id: 0
owner id: 3501
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is the consensus RBS for Bacillus subtilis.
mutable notes: The part must be 8 nucleotides away from the first methionine of the coding sequence, and so we added 'TGT' downstream of the actual binding sequence: AAAGGAGG. This, combined with the truncated non-CDS BioBrick prefix scar, gives exactly 8 'spacer bases' between the 3' end of the RBS and the start of the coding sequence.
mutable provenance: This part was synthesized directly.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: B. subtilis consensus RBS
sequence: https://synbiohub.org/public/igem/BBa_K090505_sequence/1

B. subtilis consensus RBS
type: SequenceAnnotation
persistent identity: annotation1992802
display id: annotation1992802
version: 1
top level: BBa_K090505
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K090505/annotation1992802/range1992802/1

https://synbiohub.org/public/igem/BBa_K090505/annotation1992802/range1992802/1
type: Range
persistent identity: range1992802
display id: range1992802
version: 1
direction: direction/forward
top level: BBa_K090505
start: 1
end: 8
orientation: inline

MDA
type: ComponentDefinition
persistent identity: BBa M36761
display id: BBa_M36761
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Melamine deaminase coding sequence
creator: Ray Paul Laureano-Mercado & Mark Kirollos
created: 2013-06-09T11:00:00Z
modified: 2015-05-08T01:14:06Z
discontinued: false
dominant: false
experience: experience/None
group u list: _848_
m user id: 0
owner id: 17944
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This part is the coding sequence for the enzyme melamine deaminase, optimized for usage in E. coli. This enzyme eliminates an amine group from melamine to produce ammonia and ammeline and is able to remove another amine group at a rate 10 times slower than the first reaction to bring the compound to ammelide. The enzyme does not react to a measurable extent with this last compound, so it metabolizes melamine by consecutive and predictable deaminations.
mutable notes: The TriA gene???s codons were modified from the original and optimized for E. coli to a Codon Adaptation Index (CAI) of .76. After that, some palindromic and mRNA destabilizing sequences were removed to increase the stability of our DNA during synthesis.
mutable provenance: The original gene coding for the protein of interest was isolated from a species of Pseudomonas (http://www.ncbi.nlm.nih.gov/nuccore/11890744).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Melamine Deaminase
sequence annotation: Stop (TAG)
sequence annotation: Start (ATG)
sequence: https://synbiohub.org/public/igem/BBa_M36761_sequence/1

Melamine Deaminase
type: SequenceAnnotation
persistent identity: annotation2329380
display id: annotation2329380
version: 1
top level: MDA
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_M36761/annotation2329380/range2329380/1

https://synbiohub.org/public/igem/BBa_M36761/annotation2329380/range2329380/1
type: Range
persistent identity: range2329380
display id: range2329380
version: 1
direction: direction/forward
top level: MDA
start: 1
end: 1422
orientation: inline

Stop (TAG)
type: SequenceAnnotation
persistent identity: annotation2329382
display id: annotation2329382
version: 1
top level: MDA
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_M36761/annotation2329382/range2329382/1

https://synbiohub.org/public/igem/BBa_M36761/annotation2329382/range2329382/1
type: Range
persistent identity: range2329382
display id: range2329382
version: 1
direction: direction/forward
top level: MDA
start: 1423
end: 1425
orientation: inline

Start (ATG)
type: SequenceAnnotation
persistent identity: annotation2329381
display id: annotation2329381
version: 1
top level: MDA
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_M36761/annotation2329381/range2329381/1

https://synbiohub.org/public/igem/BBa_M36761/annotation2329381/range2329381/1
type: Range
persistent identity: range2329381
display id: range2329381
version: 1
direction: direction/forward
top level: MDA
start: 1
end: 3
orientation: inline

BBa_K864400
type: ComponentDefinition
persistent identity: BBa K864400
display id: BBa_K864400
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Ptac, trp & lac regulated promoter
creator: Erik Lundin
created: 2012-09-24T11:00:00Z
modified: 2015-05-08T01:13:37Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1124_
m user id: 0
owner id: 9827
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: The Ptac promoter is a functional hybrid promoter, derived from the trp and lac promoters, that are regulated by trp and lac [1]. This part also exist together with lacI, part BBa_K180000 [1] Proc. Natl. Acad. Sci. USA, Vol. 80, pp. 21-25
mutable notes: Oligos ordered from SigmaAldrich were annealed to form a double stranded DNA segment, ready to be ligated into any BioBrick backbone.
mutable provenance: Created from oligos ordered from SigmaAldrich. Oligo sequences: Ptac+ AATTCGCGGCCGCTTCTAGAGGAGCTGTTGACAATTAATCATCGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTA Ptac- CTAGTAAATTGTTATCCGCTCACAATTCCACACATTATACGAGCCGATGATTAATTGTCAACAGCTCCTCTAGAAGCGGCCGCG
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: lacO1 site
sequence annotation: -10
sequence annotation: -35
sequence annotation: Ptac
sequence: https://synbiohub.org/public/igem/BBa_K864400_sequence/1

lacO1 site
type: SequenceAnnotation
persistent identity: annotation2197240
display id: annotation2197240
version: 1
top level: BBa_K864400
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K864400/annotation2197240/range2197240/1

https://synbiohub.org/public/igem/BBa_K864400/annotation2197240/range2197240/1
type: Range
persistent identity: range2197240
display id: range2197240
version: 1
direction: direction/forward
top level: BBa_K864400
start: 36
end: 61
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2197238
display id: annotation2197238
version: 1
top level: BBa_K864400
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K864400/annotation2197238/range2197238/1

https://synbiohub.org/public/igem/BBa_K864400/annotation2197238/range2197238/1
type: Range
persistent identity: range2197238
display id: range2197238
version: 1
direction: direction/forward
top level: BBa_K864400
start: 29
end: 33
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2197237
display id: annotation2197237
version: 1
top level: BBa_K864400
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K864400/annotation2197237/range2197237/1

https://synbiohub.org/public/igem/BBa_K864400/annotation2197237/range2197237/1
type: Range
persistent identity: range2197237
display id: range2197237
version: 1
direction: direction/forward
top level: BBa_K864400
start: 7
end: 12
orientation: inline

Ptac
type: SequenceAnnotation
persistent identity: annotation2197236
display id: annotation2197236
version: 1
top level: BBa_K864400
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K864400/annotation2197236/range2197236/1

https://synbiohub.org/public/igem/BBa_K864400/annotation2197236/range2197236/1
type: Range
persistent identity: range2197236
display id: range2197236
version: 1
direction: direction/forward
top level: BBa_K864400
start: 1
end: 40
orientation: inline

BBa_K1585101
type: ComponentDefinition
persistent identity: BBa K1585101
display id: BBa_K1585101
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Anderson Promoter with lacI binding site
creator: Tobias Schwanemann
created: 2015-08-23T11:00:00Z
modified: 2015-09-01T03:27:19Z
discontinued: false
dominant: false
experience: experience/Fails
group u list: _2002_
m user id: 24474
owner id: 24474
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Promoters of the Anderson Promoter Collection with lacI binding site derived from [http://parts.igem.org/Part:BBa_R0010 R_0010] to make them [http://openwetware.org/wiki/IPTG IPTG] iducible.
mutable notes: The "lacI binding site" of Part [http://parts.igem.org/Part:BBa_R0010 R_0010] differs from lacO from Part [http://parts.igem.org/Part:BBa_J33207 BBa_J33207] although they should be same. We choosed part BBa_R0010 because we assumed older parts would be reliable.
mutable provenance: Assambly of [http://parts.igem.org/Part:BBa_J23100 J23100] and lacI-binding-site of [http://parts.igem.org/Part:BBa_R0010 R_0010]
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
component: BBa_J23101
component: BBa_K1585999
sequence annotation: BBa_J23101
sequence annotation: BBa_K1585999
sequence: https://synbiohub.org/public/igem/BBa_K1585101_sequence/1

BBa_J23101
type: Component
persistent identity: component2436906
display id: component2436906
version: 1
top level: BBa_K1585101
access: public
definition: https://synbiohub.org/public/igem/BBa_J23101/1

BBa_K1585999
type: Component
persistent identity: component2436907
display id: component2436907
version: 1
top level: BBa_K1585101
access: public
definition: https://synbiohub.org/public/igem/BBa_K1585999/1

BBa_J23101
type: SequenceAnnotation
persistent identity: annotation2436906
display id: annotation2436906
version: 1
top level: BBa_K1585101
location: https://synbiohub.org/public/igem/BBa_K1585101/annotation2436906/range2436906/1
component: BBa_J23101

https://synbiohub.org/public/igem/BBa_K1585101/annotation2436906/range2436906/1
type: Range
persistent identity: range2436906
display id: range2436906
version: 1
direction: direction/reverse
top level: BBa_K1585101
start: 1
end: 35
orientation: inline

BBa_K1585999
type: SequenceAnnotation
persistent identity: annotation2436907
display id: annotation2436907
version: 1
top level: BBa_K1585101
location: https://synbiohub.org/public/igem/BBa_K1585101/annotation2436907/range2436907/1
component: BBa_K1585999

https://synbiohub.org/public/igem/BBa_K1585101/annotation2436907/range2436907/1
type: Range
persistent identity: range2436907
display id: range2436907
version: 1
direction: direction/reverse
top level: BBa_K1585101
start: 44
end: 78
orientation: inline

hybB
type: ComponentDefinition
persistent identity: BBa J45503
display id: BBa_J45503
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: hybB Cold Shock Promoter
creator: Stephen Payne
created: 2006-06-20T11:00:00Z
modified: 2015-08-31T04:08:49Z
discontinued: false
dominant: false
experience: experience/None
group u list: _84_
m user id: 0
owner id: 642
owning group id: 84
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This cold shock promoter is only active at temperatures lower than 30 degree Celsius.
mutable notes: None.
mutable provenance: Chris Voigt Lab at UCSF
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J45503_sequence/1

HSP
type: ComponentDefinition
persistent identity: BBa K338001
display id: BBa_K338001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Heat Shock Promoter (HSP)
creator: 2010 Caltech iGEM Team
created: 2010-10-23T11:00:00Z
modified: 2015-05-08T01:12:07Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _454_
m user id: 0
owner id: 6189
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This is a modified K112400 heat shock promoter in BBa standard.
mutable notes: None
mutable provenance: Modified BBa_K112400
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K338001_sequence/1

BBa_J61119
type: ComponentDefinition
persistent identity: BBa J61119
display id: BBa_J61119
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Ribosome Binding Site Family Member
creator: John Anderson
created: 2007-04-24T11:00:00Z
modified: 2015-08-31T01:59:49Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: false
mutable description: {{JCA_Arkin_RBSFamily}}
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J61119_sequence/1

NAND2616
type: ComponentDefinition
persistent identity: BBa I732301
display id: BBa_I732301
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: NAND Candidate (U073O26D001O16)
creator: Ma Rui
created: 2007-10-02T11:00:00Z
modified: 2015-08-31T04:07:57Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _156_
m user id: 0
owner id: 1592
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: U97NUL D001O11
mutable notes: N/A
mutable provenance: LacIq
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732301_sequence/1

BBa_B1002
type: ComponentDefinition
persistent identity: BBa B1002
display id: BBa_B1002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Terminator (artificial, small, %T~=85%)
creator: Haiyao Huang
created: 2006-08-29T11:00:00Z
modified: 2015-08-31T04:07:21Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _41_
m user id: 0
owner id: 745
owning group id: 41
sample status: In stock
status: status/Available
bookmark: false
mutable description: Artifical terminator, estimated %T~=85
mutable notes: Bidirectional, with the reverse estimated to be less effective than the forward. Has a polyA tail of 6 residues.
mutable provenance: antiquity
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: B1002
sequence annotation: Poly A tail
sequence annotation: stem loop
sequence: https://synbiohub.org/public/igem/BBa_B1002_sequence/1

B1002
type: SequenceAnnotation
persistent identity: annotation1898412
display id: annotation1898412
version: 1
top level: BBa_B1002
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_B1002/annotation1898412/range1898412/1

https://synbiohub.org/public/igem/BBa_B1002/annotation1898412/range1898412/1
type: Range
persistent identity: range1898412
display id: range1898412
version: 1
direction: direction/forward
top level: BBa_B1002
start: 1
end: 34
orientation: inline

Poly A tail
type: SequenceAnnotation
persistent identity: annotation1898414
display id: annotation1898414
version: 1
top level: BBa_B1002
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_B1002/annotation1898414/range1898414/1

https://synbiohub.org/public/igem/BBa_B1002/annotation1898414/range1898414/1
type: Range
persistent identity: range1898414
display id: range1898414
version: 1
direction: direction/forward
top level: BBa_B1002
start: 25
end: 31
orientation: inline

Poly A tail
type: SequenceAnnotation
persistent identity: annotation1898415
display id: annotation1898415
version: 1
top level: BBa_B1002
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_B1002/annotation1898415/range1898415/1

https://synbiohub.org/public/igem/BBa_B1002/annotation1898415/range1898415/1
type: Range
persistent identity: range1898415
display id: range1898415
version: 1
direction: direction/reverse
top level: BBa_B1002
start: 4
end: 9
orientation: inline

stem loop
type: SequenceAnnotation
persistent identity: annotation1898413
display id: annotation1898413
version: 1
top level: BBa_B1002
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_B1002/annotation1898413/range1898413/1

https://synbiohub.org/public/igem/BBa_B1002/annotation1898413/range1898413/1
type: Range
persistent identity: range1898413
display id: range1898413
version: 1
direction: direction/forward
top level: BBa_B1002
start: 10
end: 25
orientation: inline

NANDbeta
type: ComponentDefinition
persistent identity: BBa I732306
display id: BBa_I732306
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: NAND Candidate (U073O11D002O22)
creator: Zhan Jian
created: 2007-10-25T11:00:00Z
modified: 2015-08-31T04:07:58Z
discontinued: false
dominant: false
experience: experience/Issues
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: {| border="1" | |align="center"| |align="center"| |}
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732306_sequence/1

BBa_K338042
type: ComponentDefinition
persistent identity: BBa K338042
display id: BBa_K338042
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: GFP Reporter Device, Heat-Shock Induced
creator: Lucas Hartsough
created: 2010-10-26T11:00:00Z
modified: 2015-05-08T01:12:07Z
discontinued: false
dominant: false
group u list: _454_
m user id: 0
owner id: 6013
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Construct assembled to allow the quantitative analysis of the heat shock promoter, K338001 . Produces GFP in response to low-temperature heat shock.
mutable notes: The type of GFP used in this construct happens to have a very long half life. If growing cultures for fluorescence measurements, grow at 30°C - any higher will activate the promoter, causing GFP to be produced.
mutable provenance: The Registry of Standard Biological Parts
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0015
component: BBa_K338001
component: BBa_B0034
component: BBa_E0040
sequence annotation: BBa_E0040
sequence annotation: BBa_B0034
sequence annotation: BBa_K338001
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_K338042_sequence/1

BBa_B0015
type: Component
persistent identity: component2104994
display id: component2104994
version: 1
top level: BBa_K338042
access: public
definition: BBa_B0015

BBa_K338001
type: Component
persistent identity: component2104983
display id: component2104983
version: 1
top level: BBa_K338042
access: public
definition: HSP

BBa_B0034
type: Component
persistent identity: component2104985
display id: component2104985
version: 1
top level: BBa_K338042
access: public
definition: BBa_B0034

BBa_E0040
type: Component
persistent identity: component2104987
display id: component2104987
version: 1
top level: BBa_K338042
access: public
definition: https://synbiohub.org/public/igem/BBa_E0040/1

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation2104987
display id: annotation2104987
version: 1
top level: BBa_K338042
location: https://synbiohub.org/public/igem/BBa_K338042/annotation2104987/range2104987/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_K338042/annotation2104987/range2104987/1
type: Range
persistent identity: range2104987
display id: range2104987
version: 1
direction: direction/reverse
top level: BBa_K338042
start: 125
end: 844
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2104985
display id: annotation2104985
version: 1
top level: BBa_K338042
location: https://synbiohub.org/public/igem/BBa_K338042/annotation2104985/range2104985/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K338042/annotation2104985/range2104985/1
type: Range
persistent identity: range2104985
display id: range2104985
version: 1
direction: direction/reverse
top level: BBa_K338042
start: 107
end: 118
orientation: inline

BBa_K338001
type: SequenceAnnotation
persistent identity: annotation2104983
display id: annotation2104983
version: 1
top level: BBa_K338042
location: https://synbiohub.org/public/igem/BBa_K338042/annotation2104983/range2104983/1
component: BBa_K338001

https://synbiohub.org/public/igem/BBa_K338042/annotation2104983/range2104983/1
type: Range
persistent identity: range2104983
display id: range2104983
version: 1
direction: direction/reverse
top level: BBa_K338042
start: 1
end: 98
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2104994
display id: annotation2104994
version: 1
top level: BBa_K338042
location: https://synbiohub.org/public/igem/BBa_K338042/annotation2104994/range2104994/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K338042/annotation2104994/range2104994/1
type: Range
persistent identity: range2104994
display id: range2104994
version: 1
direction: direction/reverse
top level: BBa_K338042
start: 853
end: 981
orientation: inline

BBa_K1486039
type: ComponentDefinition
persistent identity: BBa K1486039
display id: BBa_K1486039
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: sfGFPC
creator: EPFL iGem team 2014
created: 2014-09-26T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: To edit
mutable notes: To edit
mutable provenance: To edit
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence: https://synbiohub.org/public/igem/BBa_K1486039_sequence/1

DMKP-P6
type: ComponentDefinition
persistent identity: BBa K678000
display id: BBa_K678000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: DMKP-P6, Aspergillus nidulans promoter
creator: DTU-Denmark-2
created: 2011-09-01T11:00:00Z
modified: 2015-05-08T01:13:04Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _882_
m user id: 0
owner id: 8341
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: Constitutive Aspergillus nidulans promoter
mutable notes: Cloned into shipping plasmid pSB1C3 with USER tails
mutable provenance: Aspergillus nidulans genomic DNA (AN1122)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: DMKP-P6
sequence annotation: Linker 1
sequence: https://synbiohub.org/public/igem/BBa_K678000_sequence/1

DMKP-P6
type: SequenceAnnotation
persistent identity: annotation2127343
display id: annotation2127343
version: 1
top level: DMKP-P6
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K678000/annotation2127343/range2127343/1

https://synbiohub.org/public/igem/BBa_K678000/annotation2127343/range2127343/1
type: Range
persistent identity: range2127343
display id: range2127343
version: 1
direction: direction/forward
top level: DMKP-P6
start: 9
end: 548
orientation: inline

Linker 1
type: SequenceAnnotation
persistent identity: annotation2127342
display id: annotation2127342
version: 1
top level: DMKP-P6
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K678000/annotation2127342/range2127342/1

https://synbiohub.org/public/igem/BBa_K678000/annotation2127342/range2127342/1
type: Range
persistent identity: range2127342
display id: range2127342
version: 1
direction: direction/forward
top level: DMKP-P6
start: 1
end: 8
orientation: inline

BBa_B0055
type: ComponentDefinition
persistent identity: BBa B0055
display id: BBa_B0055
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: -- No description --
creator: Reshma Shetty
created: 2005-11-16T12:00:00Z
modified: 2015-08-31T04:07:20Z
discontinued: false
dominant: false
experience: experience/None
group u list: _41_6_
m user id: 0
owner id: 126
owning group id: 44
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Terminator to be placed upstream of multiple cloning site in new pSB series plasmids.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: stem loop from bacteriophage T3
sequence annotation: identical to bacteriophage T3
sequence: https://synbiohub.org/public/igem/BBa_B0055_sequence/1

stem loop from bacteriophage T3
type: SequenceAnnotation
persistent identity: annotation1783301
display id: annotation1783301
version: 1
top level: BBa_B0055
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_B0055/annotation1783301/range1783301/1

https://synbiohub.org/public/igem/BBa_B0055/annotation1783301/range1783301/1
type: Range
persistent identity: range1783301
display id: range1783301
version: 1
direction: direction/reverse
top level: BBa_B0055
start: 36
end: 57
orientation: inline

identical to bacteriophage T3
type: SequenceAnnotation
persistent identity: annotation1783311
display id: annotation1783311
version: 1
top level: BBa_B0055
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_B0055/annotation1783311/range1783311/1

https://synbiohub.org/public/igem/BBa_B0055/annotation1783311/range1783311/1
type: Range
persistent identity: range1783311
display id: range1783311
version: 1
direction: direction/reverse
top level: BBa_B0055
start: 28
end: 66
orientation: inline

Las CIO
type: ComponentDefinition
persistent identity: BBa I14016
display id: BBa_I14016
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: P(Las) CIO
creator: Vikram Vijayan, Allen Hsu, Lawrence Fomundam
created: 2004-07-29T11:00:00Z
modified: 2015-08-31T04:07:37Z
discontinued: false
dominant: false
experience: experience/None
group u list: _4_
m user id: 0
owner id: 171
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: LasR and 3OC12HSL regulated promoter with CI Operator 1 (binding site for lambda cI repressor)
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -10
sequence annotation: R0079
sequence annotation: CI lambda O1
sequence annotation: -35
sequence: https://synbiohub.org/public/igem/BBa_I14016_sequence/1

-10
type: SequenceAnnotation
persistent identity: annotation1026125
display id: annotation1026125
version: 1
top level: Las CIO
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I14016/annotation1026125/range1026125/1

https://synbiohub.org/public/igem/BBa_I14016/annotation1026125/range1026125/1
type: Range
persistent identity: range1026125
display id: range1026125
version: 1
direction: direction/forward
top level: Las CIO
start: 140
end: 145
orientation: inline

R0079
type: SequenceAnnotation
persistent identity: annotation1017859
display id: annotation1017859
version: 1
top level: Las CIO
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_I14016/annotation1017859/range1017859/1

https://synbiohub.org/public/igem/BBa_I14016/annotation1017859/range1017859/1
type: Range
persistent identity: range1017859
display id: range1017859
version: 1
direction: direction/forward
top level: Las CIO
start: 1
end: 151
orientation: inline

CI lambda O1
type: SequenceAnnotation
persistent identity: annotation1017878
display id: annotation1017878
version: 1
top level: Las CIO
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I14016/annotation1017878/range1017878/1

https://synbiohub.org/public/igem/BBa_I14016/annotation1017878/range1017878/1
type: Range
persistent identity: range1017878
display id: range1017878
version: 1
direction: direction/forward
top level: Las CIO
start: 152
end: 168
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1026124
display id: annotation1026124
version: 1
top level: Las CIO
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I14016/annotation1026124/range1026124/1

https://synbiohub.org/public/igem/BBa_I14016/annotation1026124/range1026124/1
type: Range
persistent identity: range1026124
display id: range1026124
version: 1
direction: direction/forward
top level: Las CIO
start: 117
end: 122
orientation: inline

cinR
type: ComponentDefinition
persistent identity: BBa R0078
display id: BBa_R0078
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter (cinR and HSL regulated)
creator: Drew Endy
created: 2004-01-29T12:00:00Z
modified: 2015-05-08T01:14:15Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable provenance: Rhizobium leguminosarum
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: stem_loop
sequence annotation: unidentified/uncharacterized CinR dependent
sequence: https://synbiohub.org/public/igem/BBa_R0078_sequence/1

stem_loop
type: SequenceAnnotation
persistent identity: annotation318250
display id: annotation318250
version: 1
top level: cinR
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_R0078/annotation318250/range318250/1

https://synbiohub.org/public/igem/BBa_R0078/annotation318250/range318250/1
type: Range
persistent identity: range318250
display id: range318250
version: 1
direction: direction/forward
top level: cinR
start: 91
end: 136
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation318244
display id: annotation318244
version: 1
top level: cinR
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_R0078/annotation318244/range318244/1

https://synbiohub.org/public/igem/BBa_R0078/annotation318244/range318244/1
type: Range
persistent identity: range318244
display id: range318244
version: 1
direction: direction/forward
top level: cinR
start: 35
end: 74
orientation: inline

unidentified/uncharacterized CinR dependent
type: SequenceAnnotation
persistent identity: annotation318234
display id: annotation318234
version: 1
top level: cinR
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_R0078/annotation318234/range318234/1

https://synbiohub.org/public/igem/BBa_R0078/annotation318234/range318234/1
type: Range
persistent identity: range318234
display id: range318234
version: 1
direction: direction/forward
top level: cinR
start: 1
end: 220
orientation: inline

BBa_K112320
type: ComponentDefinition
persistent identity: BBa K112320
display id: BBa_K112320
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: {< ftsAZ promoter >} in BBb format
creator: Aron Lau
created: 2008-10-21T11:00:00Z
modified: 2015-05-08T01:09:16Z
discontinued: false
dominant: false
group u list: _224_
m user id: 0
owner id: 2999
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: ftsAZ promoter is a promoter with levels of expression are inversely proportional to the growth rate. This part is actually in BBb format. ---- This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at: [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
mutable notes: PCR al0021/al0022 on MG1655 (804bp, EcoRI/BamHI/DpnI) Sub into pBca1256 (EcoRI/BamHI, 2472+910, L) Product is pBca1256-K112320 ----------------------------------------------- al0021 Forward Biobricking of ftsAZ promoter CCATAGAATTCatgAGATCTaacgacagtgttggtgagcg al0022 Reverse Biobricking of ftsAZ promoter CGttaGGATCCgtaccaatctccagtcctactaccag
mutable provenance: MG1655
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K112320_sequence/1

BBa_I723019
type: ComponentDefinition
persistent identity: BBa I723019
display id: BBa_I723019
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS for XylR
creator: Scott Ramsay
created: 2007-10-24T11:00:00Z
modified: 2015-08-31T04:07:54Z
discontinued: false
dominant: false
experience: experience/None
group u list: _126_
m user id: 0
owner id: 2140
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is the naturally-occurring RBS found in the regulatory region of the DntR gene. gataaaaacaagaggaaaacaa
mutable notes: [Chistine/Maia]
mutable provenance: [Christine/Maia]
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_I723019_sequence/1

RBS
type: SequenceAnnotation
persistent identity: annotation1955263
display id: annotation1955263
version: 1
top level: BBa_I723019
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_I723019/annotation1955263/range1955263/1

https://synbiohub.org/public/igem/BBa_I723019/annotation1955263/range1955263/1
type: Range
persistent identity: range1955263
display id: range1955263
version: 1
direction: direction/forward
top level: BBa_I723019
start: 1
end: 20
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS (Elowitz 1999) -- defines RBS efficiency
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: RBS based on Elowitz repressilator.
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
direction: direction/forward
top level: BBa_B0034
start: 5
end: 8
orientation: inline

BBa_K1486028
type: ComponentDefinition
persistent identity: BBa K1486028
display id: BBa_K1486028
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Yeast optimized sfGFP N-terminus (1-214)
creator: EPFL iGem team 2014
created: 2014-09-26T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: split N term of sfGFP To edit
mutable notes: To edit
mutable provenance: To edit
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000324
role: partType/Tag
sequence annotation: yeast optimized sfGFP N-terminus
sequence: https://synbiohub.org/public/igem/BBa_K1486028_sequence/1

yeast optimized sfGFP N-terminus
type: SequenceAnnotation
persistent identity: annotation2417081
display id: annotation2417081
version: 1
top level: BBa_K1486028
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1486028/annotation2417081/range2417081/1

https://synbiohub.org/public/igem/BBa_K1486028/annotation2417081/range2417081/1
type: Range
persistent identity: range2417081
display id: range2417081
version: 1
direction: direction/forward
top level: BBa_K1486028
start: 1
end: 642
orientation: inline

BBa_J23116
type: ComponentDefinition
persistent identity: BBa J23116
display id: BBa_J23116
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: constitutive promoter family member
creator: John Anderson
created: 2006-08-16T11:00:00Z
modified: 2015-08-31T04:08:40Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: true
mutable description: Later
mutable notes: N/A
mutable provenance: Later
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J23116_sequence/1

BBa_K1660005
type: ComponentDefinition
persistent identity: BBa K1660005
display id: BBa_K1660005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RFP controlled by the PompR promoter
creator: Dai Yuanyi
created: 2015-09-07T11:00:00Z
modified: 2015-09-08T12:26:12Z
discontinued: false
dominant: false
group u list: _2078_
m user id: 26933
owner id: 26933
owning group id: 9
status: status/Planning
bookmark: false
mutable description: PompR is a OmpR-controlled promoter which can be positively regulated by phosphorylated OmpR. This promoter is taken from the upstream region of ompC. Phosphorylated OmpR binds to the three operator sites and activates transcription. We insert PompR(BBa_R0082) sequences into the upstream region of rfp gene, which together is connected to vector PSB1C3 afterwards, and therefore we are able to observe the OmpR promoter efficiency through the control of light.
mutable notes: We constructed this biobrick through pcr.
mutable provenance: This part comes from the stock.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_R0082
component: BBa_K516032
sequence annotation: BBa_R0082
sequence annotation: BBa_K516032
sequence: https://synbiohub.org/public/igem/BBa_K1660005_sequence/1

BBa_R0082
type: Component
persistent identity: component2446043
display id: component2446043
version: 1
top level: BBa_K1660005
access: public
definition: OmpR

BBa_K516032
type: Component
persistent identity: component2446057
display id: component2446057
version: 1
top level: BBa_K1660005
access: public
definition: https://synbiohub.org/public/igem/BBa_K516032/1

BBa_R0082
type: SequenceAnnotation
persistent identity: annotation2446043
display id: annotation2446043
version: 1
top level: BBa_K1660005
location: https://synbiohub.org/public/igem/BBa_K1660005/annotation2446043/range2446043/1
component: BBa_R0082

https://synbiohub.org/public/igem/BBa_K1660005/annotation2446043/range2446043/1
type: Range
persistent identity: range2446043
display id: range2446043
version: 1
direction: direction/reverse
top level: BBa_K1660005
start: 1
end: 108
orientation: inline

BBa_K516032
type: SequenceAnnotation
persistent identity: annotation2446057
display id: annotation2446057
version: 1
top level: BBa_K1660005
location: https://synbiohub.org/public/igem/BBa_K1660005/annotation2446057/range2446057/1
component: BBa_K516032

https://synbiohub.org/public/igem/BBa_K1660005/annotation2446057/range2446057/1
type: Range
persistent identity: range2446057
display id: range2446057
version: 1
direction: direction/reverse
top level: BBa_K1660005
start: 117
end: 978
orientation: inline

BBa_J61128
type: ComponentDefinition
persistent identity: BBa J61128
display id: BBa_J61128
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Ribosome Binding Site Family Member
creator: John Anderson
created: 2007-04-24T11:00:00Z
modified: 2015-08-31T01:59:49Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: false
mutable description: {{JCA_Arkin_RBSFamily}}
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J61128_sequence/1

BBa_S03385
type: ComponentDefinition
persistent identity: BBa S03385
display id: BBa_S03385
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Cold-sensing promoter (hybB)
creator: Elizabeth Clarke
created: 2005-11-03T12:00:00Z
modified: 2015-05-08T01:14:22Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _9_
m user id: 0
owner id: 473
owning group id: 9
sample status: No part sequence
status: status/Planning
bookmark: false
mutable description: This is the hybB promoter, which normally controlls hydrogenase regulation at low temperatures. It turns ON at 30oC and is active as low as 20oC (lower not tested). It is OFF at 37 and higher temperatures. In the literature, it is also active in anaerobic environments. This hasn't been tested.
mutable provenance: lizjaneclarke@gmail.com Univerity of California - San Francisco
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory

BBa_I756014
type: ComponentDefinition
persistent identity: BBa I756014
display id: BBa_I756014
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LexAoperator-MajorLatePromoter
creator: Johannes Hugo Decker
created: 2007-08-15T11:00:00Z
modified: 2015-08-31T04:08:06Z
discontinued: false
dominant: false
group u list: _108_
m user id: 0
owner id: 2071
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This part contains the LexAoperator and MajorLatePromoter genes with restriction site KdeI at the 5 prime end and AscI at the 3' end. Will use it as both a regulatory part for certain signals as well as a promoter.
mutable notes: Had to make sure the restriction sites used were not in the sequence or in the sequence of any of the genes downstream of the promoter.
mutable provenance: genbank
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I756014_sequence/1

BBa_K108016
type: ComponentDefinition
persistent identity: BBa K108016
display id: BBa_K108016
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PP
creator: Qi Liu
created: 2008-10-22T11:00:00Z
modified: 2015-05-08T01:09:04Z
discontinued: false
dominant: false
experience: experience/None
group u list: _222_
m user id: 0
owner id: 2671
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: promoter of Alcaligenes eutrophus PhaP gene
mutable notes: PCR using primers with biobrick prefix and surfix, and then cloned into pSB1AC3.
mutable provenance: Alcaligenes eutrophus H16 genome
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: PP
sequence: https://synbiohub.org/public/igem/BBa_K108016_sequence/1

PP
type: SequenceAnnotation
persistent identity: annotation1986961
display id: annotation1986961
version: 1
top level: BBa_K108016
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K108016/annotation1986961/range1986961/1

https://synbiohub.org/public/igem/BBa_K108016/annotation1986961/range1986961/1
type: Range
persistent identity: range1986961
display id: range1986961
version: 1
direction: direction/forward
top level: BBa_K108016
start: 1
end: 406
orientation: inline

NAND59
type: ComponentDefinition
persistent identity: BBa I732305
display id: BBa_I732305
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: NAND Candidate (U073O22D059O46)
creator: Ma Rui
created: 2007-10-02T11:00:00Z
modified: 2015-08-31T04:07:58Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _156_
m user id: 0
owner id: 1592
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: U97NUL D001O55
mutable notes: .
mutable provenance: .
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732305_sequence/1

BBa_B2040
type: ComponentDefinition
persistent identity: BBa B2040
display id: BBa_B2040
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: 8 RBS from T7
creator: Bartholomew Canton
created: 2007-05-22T11:00:00Z
modified: 2015-08-31T04:07:21Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_
m user id: 0
owner id: 135
owning group id: 84
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: to be added automatically later
mutable notes: to be added automatically later
mutable provenance: to be added automatically later
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_B2040_sequence/1

Pveg
type: ComponentDefinition
persistent identity: BBa K143012
display id: BBa_K143012
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter veg a constitutive promoter for B. subtilis
creator: James Chappell
created: 2008-09-10T11:00:00Z
modified: 2015-05-08T01:10:23Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _199_
m user id: 0
owner id: 2090
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Pveg is a constitutive promoter that constitutively expresses the P43 protein in ''B.subtilis''. Pveg contains binding sites for the ''B.sutbilis'' major sigma factor#1. Pveg in ''B.subtilis'' utilises two binding sites to cause high expression of genes#2, however our Pveg is lacking the upstream site to give a medium level of gene expression. It has been noted that the sporulation master regulatoion factor spoOA interacts with Pveg though it is not known how#3. The context with which we used the promoter Pveg is as a '''Polymerase Per Second''' (PoPS) generator.
mutable notes: The biobrick part was designed to include a single binding site for the ''B.subtilis major sigma factor. In addition the biobrick standard was applied to the promoter Pveg sequence.
mutable provenance: The Pveg promoter was suggested to us by Dr. Jan-Willem Veening of Newcastle University. This sequence supplied was compared to that of the DBTBS database#3 then a section containing the binding site synthesised by Geneart.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: Sigma A-35
sequence annotation: Sigma A -10
sequence: https://synbiohub.org/public/igem/BBa_K143012_sequence/1

Sigma A-35
type: SequenceAnnotation
persistent identity: annotation1975704
display id: annotation1975704
version: 1
top level: Pveg
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K143012/annotation1975704/range1975704/1

https://synbiohub.org/public/igem/BBa_K143012/annotation1975704/range1975704/1
type: Range
persistent identity: range1975704
display id: range1975704
version: 1
direction: direction/forward
top level: Pveg
start: 63
end: 68
orientation: inline

Sigma A -10
type: SequenceAnnotation
persistent identity: annotation1975705
display id: annotation1975705
version: 1
top level: Pveg
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K143012/annotation1975705/range1975705/1

https://synbiohub.org/public/igem/BBa_K143012/annotation1975705/range1975705/1
type: Range
persistent identity: range1975705
display id: range1975705
version: 1
direction: direction/forward
top level: Pveg
start: 86
end: 91
orientation: inline

U097NUL+D0
type: ComponentDefinition
persistent identity: BBa I732410
display id: BBa_I732410
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Family Member (U097NUL+D038O22)
creator: Zhan Jian
created: 2007-09-22T11:00:00Z
modified: 2015-08-31T04:07:58Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1572
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: To be later edited if time permits.
mutable notes: To be later edited if time permits.
mutable provenance: To be later edited if time permits.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: O2
sequence annotation: consensus elements
sequence annotation: +1
sequence: https://synbiohub.org/public/igem/BBa_I732410_sequence/1

O2
type: SequenceAnnotation
persistent identity: annotation1944559
display id: annotation1944559
version: 1
top level: U097NUL+D0
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_I732410/annotation1944559/range1944559/1

https://synbiohub.org/public/igem/BBa_I732410/annotation1944559/range1944559/1
type: Range
persistent identity: range1944559
display id: range1944559
version: 1
direction: direction/forward
top level: U097NUL+D0
start: 134
end: 153
orientation: inline

consensus elements
type: SequenceAnnotation
persistent identity: annotation1944558
display id: annotation1944558
version: 1
top level: U097NUL+D0
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I732410/annotation1944558/range1944558/1

https://synbiohub.org/public/igem/BBa_I732410/annotation1944558/range1944558/1
type: Range
persistent identity: range1944558
display id: range1944558
version: 1
direction: direction/forward
top level: U097NUL+D0
start: 67
end: 137
orientation: inline

+1
type: SequenceAnnotation
persistent identity: annotation1944560
display id: annotation1944560
version: 1
top level: U097NUL+D0
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_I732410/annotation1944560/range1944560/1

https://synbiohub.org/public/igem/BBa_I732410/annotation1944560/range1944560/1
type: Range
persistent identity: range1944560
display id: range1944560
version: 1
direction: direction/forward
top level: U097NUL+D0
start: 137
end: 137
orientation: inline

BBa_K180016
type: ComponentDefinition
persistent identity: BBa K180016
display id: BBa_K180016
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Rock-paper-scissors - Paper secondary plasmid [lux pR, LacI generator]
creator: Richard Wilson, Stephanie Chambers
created: 2009-10-18T11:00:00Z
modified: 2015-05-08T01:11:06Z
discontinued: false
dominant: false
experience: experience/None
group u list: _278_
m user id: 0
owner id: 4082
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: iGEM2009 Team:Southampton rock-paper-scissors 'Rock' secondary plasmid.
mutable notes: Regulated expression of LacI (C0012) by Lux pR (R0062).
mutable provenance: See relevant basic biobrick parts (R0062, B0034, C0012, B0015).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0015
component: BBa_B0034
component: BBa_C0012
component: BBa_R0062
sequence annotation: BBa_B0015
sequence annotation: BBa_B0034
sequence annotation: BBa_C0012
sequence annotation: BBa_R0062
sequence: https://synbiohub.org/public/igem/BBa_K180016_sequence/1

BBa_B0015
type: Component
persistent identity: component2256451
display id: component2256451
version: 1
top level: BBa_K180016
access: public
definition: BBa_B0015

BBa_B0034
type: Component
persistent identity: component2256440
display id: component2256440
version: 1
top level: BBa_K180016
access: public
definition: BBa_B0034

BBa_C0012
type: Component
persistent identity: component2256442
display id: component2256442
version: 1
top level: BBa_K180016
access: public
definition: https://synbiohub.org/public/igem/BBa_C0012/1

BBa_R0062
type: Component
persistent identity: component2256434
display id: component2256434
version: 1
top level: BBa_K180016
access: public
definition: lux pR

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2256451
display id: annotation2256451
version: 1
top level: BBa_K180016
location: https://synbiohub.org/public/igem/BBa_K180016/annotation2256451/range2256451/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K180016/annotation2256451/range2256451/1
type: Range
persistent identity: range2256451
display id: range2256451
version: 1
direction: direction/reverse
top level: BBa_K180016
start: 1243
end: 1371
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2256440
display id: annotation2256440
version: 1
top level: BBa_K180016
location: https://synbiohub.org/public/igem/BBa_K180016/annotation2256440/range2256440/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K180016/annotation2256440/range2256440/1
type: Range
persistent identity: range2256440
display id: range2256440
version: 1
direction: direction/reverse
top level: BBa_K180016
start: 64
end: 75
orientation: inline

BBa_C0012
type: SequenceAnnotation
persistent identity: annotation2256442
display id: annotation2256442
version: 1
top level: BBa_K180016
location: https://synbiohub.org/public/igem/BBa_K180016/annotation2256442/range2256442/1
component: BBa_C0012

https://synbiohub.org/public/igem/BBa_K180016/annotation2256442/range2256442/1
type: Range
persistent identity: range2256442
display id: range2256442
version: 1
direction: direction/forward
top level: BBa_K180016
start: 82
end: 1209
orientation: inline

BBa_R0062
type: SequenceAnnotation
persistent identity: annotation2256434
display id: annotation2256434
version: 1
top level: BBa_K180016
location: https://synbiohub.org/public/igem/BBa_K180016/annotation2256434/range2256434/1
component: BBa_R0062

https://synbiohub.org/public/igem/BBa_K180016/annotation2256434/range2256434/1
type: Range
persistent identity: range2256434
display id: range2256434
version: 1
direction: direction/forward
top level: BBa_K180016
start: 1
end: 55
orientation: inline

BBa_K1895001
type: ComponentDefinition
persistent identity: BBa K1895001
display id: BBa_K1895001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: rpoH
creator: Lauren Mills
created: 2016-07-07T11:00:00Z
modified: 2016-08-03T07:04:22Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2361_
m user id: 31559
owner id: 31475
owning group id: 9
status: status/Planning
bookmark: false
mutable description: wefWD
mutable notes: awefWv
mutable provenance: AWwvf
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_K1895001_sequence/1

BBa_I746363
type: ComponentDefinition
persistent identity: BBa I746363
display id: BBa_I746363
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PV promoter from P2 phage
creator: Stefan Milde
created: 2007-09-10T11:00:00Z
modified: 2015-08-31T04:08:04Z
discontinued: false
dominant: false
group u list: _116_
m user id: 0
owner id: 2122
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is the PF promoter taken from the P2 phage genome. It is an inducible promoter that is activated by a class of activators, including P2 ogr (I746350), PSP3 pag (I746351) and phiR73 delta (I746352). These different activators should cause different levels of activity of the PF promoter.
mutable notes: no special considerations
mutable provenance: P2 phage genome
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: PV
sequence: https://synbiohub.org/public/igem/BBa_I746363_sequence/1

PV
type: SequenceAnnotation
persistent identity: annotation1943786
display id: annotation1943786
version: 1
top level: BBa_I746363
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I746363/annotation1943786/range1943786/1

https://synbiohub.org/public/igem/BBa_I746363/annotation1943786/range1943786/1
type: Range
persistent identity: range1943786
display id: range1943786
version: 1
direction: direction/forward
top level: BBa_I746363
start: 1
end: 91
orientation: inline

cII p22
type: ComponentDefinition
persistent identity: BBa R1053
display id: BBa_R1053
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter, Standard (p22 cII regulated)
creator: Maia Mahoney, Drew Endy
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:15Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: [Note: This is the same part as R0053 except that the -10 and -35 sites and spacing have been changed to comply with BBa_S0001]. The p22 cII regulatory region sequence is a 97 base-pair sequence with the standard BioBrick prefix and suffix sections on its ends. p22 cII repressor protein, BBa_C0053, binds to it. This segment contains O-R1, O-R2, a fragment of O-R3, the -35 of P-RM, and P-R (-10 and -35 from Tom Knight)
mutable notes: Modified from wild-type to comply with BBa_S0001: TTGACA-17N-GATACT
mutable provenance: Bacteriophage p22.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -10
sequence annotation: OR2
sequence annotation: OR3
sequence annotation: -35
sequence annotation: OR1
sequence annotation: BBa_R1053
sequence: https://synbiohub.org/public/igem/BBa_R1053_sequence/1

-10
type: SequenceAnnotation
persistent identity: annotation2095
display id: annotation2095
version: 1
top level: cII p22
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R1053/annotation2095/range2095/1

https://synbiohub.org/public/igem/BBa_R1053/annotation2095/range2095/1
type: Range
persistent identity: range2095
display id: range2095
version: 1
direction: direction/forward
top level: cII p22
start: 31
end: 36
orientation: inline

OR2
type: SequenceAnnotation
persistent identity: annotation2089
display id: annotation2089
version: 1
top level: cII p22
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R1053/annotation2089/range2089/1

https://synbiohub.org/public/igem/BBa_R1053/annotation2089/range2089/1
type: Range
persistent identity: range2089
display id: range2089
version: 1
direction: direction/forward
top level: cII p22
start: 11
end: 29
orientation: inline

OR3
type: SequenceAnnotation
persistent identity: annotation2088
display id: annotation2088
version: 1
top level: cII p22
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R1053/annotation2088/range2088/1

https://synbiohub.org/public/igem/BBa_R1053/annotation2088/range2088/1
type: Range
persistent identity: range2088
display id: range2088
version: 1
direction: direction/forward
top level: cII p22
start: 1
end: 3
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2094
display id: annotation2094
version: 1
top level: cII p22
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R1053/annotation2094/range2094/1

https://synbiohub.org/public/igem/BBa_R1053/annotation2094/range2094/1
type: Range
persistent identity: range2094
display id: range2094
version: 1
direction: direction/forward
top level: cII p22
start: 8
end: 14
orientation: inline

OR1
type: SequenceAnnotation
persistent identity: annotation2090
display id: annotation2090
version: 1
top level: cII p22
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R1053/annotation2090/range2090/1

https://synbiohub.org/public/igem/BBa_R1053/annotation2090/range2090/1
type: Range
persistent identity: range2090
display id: range2090
version: 1
direction: direction/forward
top level: cII p22
start: 35
end: 52
orientation: inline

BBa_R1053
type: SequenceAnnotation
persistent identity: annotation7076
display id: annotation7076
version: 1
top level: cII p22
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_R1053/annotation7076/range7076/1

https://synbiohub.org/public/igem/BBa_R1053/annotation7076/range7076/1
type: Range
persistent identity: range7076
display id: range7076
version: 1
direction: direction/forward
top level: cII p22
start: 1
end: 55
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2091
display id: annotation2091
version: 1
top level: cII p22
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R1053/annotation2091/range2091/1

https://synbiohub.org/public/igem/BBa_R1053/annotation2091/range2091/1
type: Range
persistent identity: range2091
display id: range2091
version: 1
direction: direction/forward
top level: cII p22
start: 19
end: 24
orientation: inline

BBa_I751500
type: ComponentDefinition
persistent identity: BBa I751500
display id: BBa_I751500
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pcI (for positive control of pcI-lux hybrid promoter)
creator: Kenichiro Iwasaki
created: 2007-10-24T11:00:00Z
modified: 2015-08-31T04:08:06Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _151_
m user id: 0
owner id: 2188
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: pcI
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -10
sequence annotation: -35
sequence annotation: pcI
sequence annotation: OR1
sequence annotation: OR3
sequence annotation: OR2
sequence: https://synbiohub.org/public/igem/BBa_I751500_sequence/1

-10
type: SequenceAnnotation
persistent identity: annotation1958480
display id: annotation1958480
version: 1
top level: BBa_I751500
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I751500/annotation1958480/range1958480/1

https://synbiohub.org/public/igem/BBa_I751500/annotation1958480/range1958480/1
type: Range
persistent identity: range1958480
display id: range1958480
version: 1
direction: direction/forward
top level: BBa_I751500
start: 47
end: 50
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1958479
display id: annotation1958479
version: 1
top level: BBa_I751500
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I751500/annotation1958479/range1958479/1

https://synbiohub.org/public/igem/BBa_I751500/annotation1958479/range1958479/1
type: Range
persistent identity: range1958479
display id: range1958479
version: 1
direction: direction/forward
top level: BBa_I751500
start: 21
end: 26
orientation: inline

pcI
type: SequenceAnnotation
persistent identity: annotation1955476
display id: annotation1955476
version: 1
top level: BBa_I751500
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I751500/annotation1955476/range1955476/1

https://synbiohub.org/public/igem/BBa_I751500/annotation1955476/range1955476/1
type: Range
persistent identity: range1955476
display id: range1955476
version: 1
direction: direction/forward
top level: BBa_I751500
start: 1
end: 77
orientation: inline

OR1
type: SequenceAnnotation
persistent identity: annotation1958484
display id: annotation1958484
version: 1
top level: BBa_I751500
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I751500/annotation1958484/range1958484/1

https://synbiohub.org/public/igem/BBa_I751500/annotation1958484/range1958484/1
type: Range
persistent identity: range1958484
display id: range1958484
version: 1
direction: direction/forward
top level: BBa_I751500
start: 51
end: 77
orientation: inline

OR3
type: SequenceAnnotation
persistent identity: annotation1958482
display id: annotation1958482
version: 1
top level: BBa_I751500
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I751500/annotation1958482/range1958482/1

https://synbiohub.org/public/igem/BBa_I751500/annotation1958482/range1958482/1
type: Range
persistent identity: range1958482
display id: range1958482
version: 1
direction: direction/forward
top level: BBa_I751500
start: 1
end: 20
orientation: inline

OR2
type: SequenceAnnotation
persistent identity: annotation1958483
display id: annotation1958483
version: 1
top level: BBa_I751500
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I751500/annotation1958483/range1958483/1

https://synbiohub.org/public/igem/BBa_I751500/annotation1958483/range1958483/1
type: Range
persistent identity: range1958483
display id: range1958483
version: 1
direction: direction/forward
top level: BBa_I751500
start: 27
end: 46
orientation: inline

BBa_K678012
type: ComponentDefinition
persistent identity: BBa K678012
display id: BBa_K678012
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: SV40 poly A, terminator for mammalian cells
creator: DTU-Denmark-2
created: 2011-09-03T11:00:00Z
modified: 2015-05-08T01:13:04Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _882_
m user id: 0
owner id: 8341
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: SV40 poly A, terminator for use in mammalian cells
mutable notes: Compatible with the Plug'n'Play assembly standard
mutable provenance: From plasmid pcDNA3.1 from Invitrogen
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: SV40
sequence annotation: Linker 4
sequence: https://synbiohub.org/public/igem/BBa_K678012_sequence/1

SV40
type: SequenceAnnotation
persistent identity: annotation2127355
display id: annotation2127355
version: 1
top level: BBa_K678012
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_K678012/annotation2127355/range2127355/1

https://synbiohub.org/public/igem/BBa_K678012/annotation2127355/range2127355/1
type: Range
persistent identity: range2127355
display id: range2127355
version: 1
direction: direction/forward
top level: BBa_K678012
start: 9
end: 139
orientation: inline

Linker 4
type: SequenceAnnotation
persistent identity: annotation2127354
display id: annotation2127354
version: 1
top level: BBa_K678012
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K678012/annotation2127354/range2127354/1

https://synbiohub.org/public/igem/BBa_K678012/annotation2127354/range2127354/1
type: Range
persistent identity: range2127354
display id: range2127354
version: 1
direction: direction/forward
top level: BBa_K678012
start: 1
end: 8
orientation: inline

BBa_J61108
type: ComponentDefinition
persistent identity: BBa J61108
display id: BBa_J61108
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Ribosome Binding Site Family Member
creator: John Anderson
created: 2007-04-23T11:00:00Z
modified: 2015-08-31T02:03:00Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: false
mutable description: {{JCA_Arkin_RBSFamily}}
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J61108_sequence/1

BBa_J61133
type: ComponentDefinition
persistent identity: BBa J61133
display id: BBa_J61133
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Ribosome Binding Site Family Member
creator: John Anderson
created: 2007-04-24T11:00:00Z
modified: 2015-08-31T01:59:49Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: false
mutable description: {{JCA_Arkin_RBSFamily}}
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J61133_sequence/1

BBa_I746362
type: ComponentDefinition
persistent identity: BBa I746362
display id: BBa_I746362
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PP promoter from P2 phage
creator: Stefan Milde
created: 2007-09-10T11:00:00Z
modified: 2015-08-31T04:08:04Z
discontinued: false
dominant: false
experience: experience/None
group u list: _116_
m user id: 0
owner id: 2122
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This is the PP promoter taken from the P2 phage genome. It is an inducible promoter that is activated by a class of activators, including P2 ogr (I746350), PSP3 pag (I746351) and phiR73 delta (I746352). These different activators should cause different levels of activity of the PP promoter.
mutable notes: no special considerations
mutable provenance: P2 phage genome
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: PP
sequence: https://synbiohub.org/public/igem/BBa_I746362_sequence/1

PP
type: SequenceAnnotation
persistent identity: annotation1943785
display id: annotation1943785
version: 1
top level: BBa_I746362
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I746362/annotation1943785/range1943785/1

https://synbiohub.org/public/igem/BBa_I746362/annotation1943785/range1943785/1
type: Range
persistent identity: range1943785
display id: range1943785
version: 1
direction: direction/forward
top level: BBa_I746362
start: 1
end: 92
orientation: inline

pRE
type: ComponentDefinition
persistent identity: BBa K116603
display id: BBa_K116603
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pRE promoter from λ phage
creator: Jesse Wu
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:09:33Z
discontinued: false
dominant: false
experience: experience/None
group u list: _210_
m user id: 0
owner id: 2749
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: The pRE coding region from λ phage. It is able to be induced by CII (BBa_K116602).
mutable notes: none.
mutable provenance: λ phage
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K116603_sequence/1

BBa_K112406
type: ComponentDefinition
persistent identity: BBa K112406
display id: BBa_K112406
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: cadC promoter
creator: Bing Xia
created: 2008-10-21T11:00:00Z
modified: 2015-05-08T01:09:17Z
discontinued: false
dominant: false
group u list: _224_
m user id: 0
owner id: 2998
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: ---- This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at: [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
mutable provenance: Biobricking of cadC promoter and cadC gene PCR cadC-F and cadC-R on MG1655 (2336 bp, EcoR1/BamH1) Sub into pBca1256 (EcoR1/BamH1 910+2472bp, L) Product is pBca1256-K112406 ---------------------------- cb10013-cadC-F cgataGAATTCatgAGATCTcaccagcaatttgcgggcatatc cb10012-cad-R cgtagGGATCCtgaccagaccaaactggcg
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K112406_sequence/1

BBa_J61118
type: ComponentDefinition
persistent identity: BBa J61118
display id: BBa_J61118
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Ribosome Binding Site Family Member
creator: John Anderson
created: 2007-04-23T11:00:00Z
modified: 2015-08-31T01:59:49Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: false
mutable description: {{JCA_Arkin_RBSFamily}}
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J61118_sequence/1

BBa_J61101
type: ComponentDefinition
persistent identity: BBa J61101
display id: BBa_J61101
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Ribosome Binding Site Family Member
creator: John Anderson
created: 2007-01-28T12:00:00Z
modified: 2015-08-31T02:03:00Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: false
mutable description: fix
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J61101_sequence/1

U073O11+D0
type: ComponentDefinition
persistent identity: BBa I732426
display id: BBa_I732426
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Family Member (U073O11+D014O22)
creator: Zhan Jian
created: 2007-10-13T11:00:00Z
modified: 2015-08-31T04:07:58Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732426_sequence/1

eGFP-TS
type: ComponentDefinition
persistent identity: BBa K678008
display id: BBa_K678008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: eGFP-TS
creator: DTU-Danmark-2
created: 2011-09-12T11:00:00Z
modified: 2015-05-08T01:13:04Z
discontinued: false
dominant: false
experience: experience/None
group u list: _882_
m user id: 0
owner id: 10556
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part was designed for the Plug'n'Play with DNA assembly standard (RFC 80), therefore this part contains tails at the 5' and 3' ends as shown on the figure below.
mutable notes: No
mutable provenance: The Danish Cancer Society
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: Kozak
sequence annotation: Linker 3
sequence annotation: eGFP-TS
sequence: https://synbiohub.org/public/igem/BBa_K678008_sequence/1

Kozak
type: SequenceAnnotation
persistent identity: annotation2127769
display id: annotation2127769
version: 1
top level: eGFP-TS
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K678008/annotation2127769/range2127769/1

https://synbiohub.org/public/igem/BBa_K678008/annotation2127769/range2127769/1
type: Range
persistent identity: range2127769
display id: range2127769
version: 1
direction: direction/forward
top level: eGFP-TS
start: 9
end: 19
orientation: inline

Linker 3
type: SequenceAnnotation
persistent identity: annotation2127768
display id: annotation2127768
version: 1
top level: eGFP-TS
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K678008/annotation2127768/range2127768/1

https://synbiohub.org/public/igem/BBa_K678008/annotation2127768/range2127768/1
type: Range
persistent identity: range2127768
display id: range2127768
version: 1
direction: direction/forward
top level: eGFP-TS
start: 1
end: 9
orientation: inline

eGFP-TS
type: SequenceAnnotation
persistent identity: annotation2127770
display id: annotation2127770
version: 1
top level: eGFP-TS
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K678008/annotation2127770/range2127770/1

https://synbiohub.org/public/igem/BBa_K678008/annotation2127770/range2127770/1
type: Range
persistent identity: range2127770
display id: range2127770
version: 1
direction: direction/forward
top level: eGFP-TS
start: 16
end: 736
orientation: inline

BBa_K1486027
type: ComponentDefinition
persistent identity: BBa K1486027
display id: BBa_K1486027
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: R.reniformis luciferase + ADH1 terminator + Kanamycin resistance
creator: EPFL iGem team 2014
created: 2014-09-26T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: To edit
mutable notes: To edit
mutable provenance: To edit
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000324
role: partType/Tag
component: BBa_K1486019
component: BBa_K1486020
component: BBa_K1486037
component: BBa_K801012
component: BBa_K1486024
sequence annotation: BBa_K1486019
sequence annotation: BBa_K1486020
sequence annotation: BBa_K1486024
sequence annotation: BBa_K801012
sequence annotation: BBa_K1486037
sequence: https://synbiohub.org/public/igem/BBa_K1486027_sequence/1

BBa_K1486019
type: Component
persistent identity: component2417707
display id: component2417707
version: 1
top level: BBa_K1486027
access: public
definition: BBa_K1486019

BBa_K1486020
type: Component
persistent identity: component2417708
display id: component2417708
version: 1
top level: BBa_K1486027
access: public
definition: BBa_K1486020

BBa_K1486037
type: Component
persistent identity: component2417706
display id: component2417706
version: 1
top level: BBa_K1486027
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486037/1

BBa_K801012
type: Component
persistent identity: component2417710
display id: component2417710
version: 1
top level: BBa_K1486027
access: public
definition: https://synbiohub.org/public/igem/BBa_K801012/1

BBa_K1486024
type: Component
persistent identity: component2417714
display id: component2417714
version: 1
top level: BBa_K1486027
access: public
definition: BBa_K1486024

BBa_K1486019
type: SequenceAnnotation
persistent identity: annotation2417707
display id: annotation2417707
version: 1
top level: BBa_K1486027
location: https://synbiohub.org/public/igem/BBa_K1486027/annotation2417707/range2417707/1
component: BBa_K1486019

https://synbiohub.org/public/igem/BBa_K1486027/annotation2417707/range2417707/1
type: Range
persistent identity: range2417707
display id: range2417707
version: 1
direction: direction/reverse
top level: BBa_K1486027
start: 40
end: 369
orientation: inline

BBa_K1486020
type: SequenceAnnotation
persistent identity: annotation2417708
display id: annotation2417708
version: 1
top level: BBa_K1486027
location: https://synbiohub.org/public/igem/BBa_K1486027/annotation2417708/range2417708/1
component: BBa_K1486020

https://synbiohub.org/public/igem/BBa_K1486027/annotation2417708/range2417708/1
type: Range
persistent identity: range2417708
display id: range2417708
version: 1
direction: direction/reverse
top level: BBa_K1486027
start: 370
end: 975
orientation: inline

BBa_K1486024
type: SequenceAnnotation
persistent identity: annotation2417714
display id: annotation2417714
version: 1
top level: BBa_K1486027
location: https://synbiohub.org/public/igem/BBa_K1486027/annotation2417714/range2417714/1
component: BBa_K1486024

https://synbiohub.org/public/igem/BBa_K1486027/annotation2417714/range2417714/1
type: Range
persistent identity: range2417714
display id: range2417714
version: 1
direction: direction/reverse
top level: BBa_K1486027
start: 1325
end: 2681
orientation: inline

BBa_K801012
type: SequenceAnnotation
persistent identity: annotation2417710
display id: annotation2417710
version: 1
top level: BBa_K1486027
location: https://synbiohub.org/public/igem/BBa_K1486027/annotation2417710/range2417710/1
component: BBa_K801012

https://synbiohub.org/public/igem/BBa_K1486027/annotation2417710/range2417710/1
type: Range
persistent identity: range2417710
display id: range2417710
version: 1
direction: direction/reverse
top level: BBa_K1486027
start: 976
end: 1324
orientation: inline

BBa_K1486037
type: SequenceAnnotation
persistent identity: annotation2417706
display id: annotation2417706
version: 1
top level: BBa_K1486027
location: https://synbiohub.org/public/igem/BBa_K1486027/annotation2417706/range2417706/1
component: BBa_K1486037

https://synbiohub.org/public/igem/BBa_K1486027/annotation2417706/range2417706/1
type: Range
persistent identity: range2417706
display id: range2417706
version: 1
direction: direction/reverse
top level: BBa_K1486027
start: 1
end: 39
orientation: inline

BBa_K1486007
type: ComponentDefinition
persistent identity: BBa K1486007
display id: BBa_K1486007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: IFP[2]
creator: iGem EPFL 2014
created: 2014-08-24T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is the second part of the split infrared fluorescent protein (IFP1.4). To be used with IFP[1].
mutable notes: When fused to another coding sequence, insert a flexible linker.
mutable provenance: From Michnick Article. To Edit
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: IFP[2]
sequence annotation: START
sequence annotation: STOP
sequence: https://synbiohub.org/public/igem/BBa_K1486007_sequence/1

IFP[2]
type: SequenceAnnotation
persistent identity: annotation2382164
display id: annotation2382164
version: 1
top level: BBa_K1486007
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1486007/annotation2382164/range2382164/1

https://synbiohub.org/public/igem/BBa_K1486007/annotation2382164/range2382164/1
type: Range
persistent identity: range2382164
display id: range2382164
version: 1
direction: direction/forward
top level: BBa_K1486007
start: 1
end: 579
orientation: inline

START
type: SequenceAnnotation
persistent identity: annotation2382128
display id: annotation2382128
version: 1
top level: BBa_K1486007
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1486007/annotation2382128/range2382128/1

https://synbiohub.org/public/igem/BBa_K1486007/annotation2382128/range2382128/1
type: Range
persistent identity: range2382128
display id: range2382128
version: 1
direction: direction/forward
top level: BBa_K1486007
start: 1
end: 3
orientation: inline

STOP
type: SequenceAnnotation
persistent identity: annotation2382163
display id: annotation2382163
version: 1
top level: BBa_K1486007
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1486007/annotation2382163/range2382163/1

https://synbiohub.org/public/igem/BBa_K1486007/annotation2382163/range2382163/1
type: Range
persistent identity: range2382163
display id: range2382163
version: 1
direction: direction/forward
top level: BBa_K1486007
start: 577
end: 579
orientation: inline

BBa_K389052
type: ComponentDefinition
persistent identity: BBa K389052
display id: BBa_K389052
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Tightly regulated lacO + mRFP
creator: Jonas Aretz
created: 2010-10-07T11:00:00Z
modified: 2015-05-08T01:12:19Z
discontinued: false
dominant: false
experience: experience/Fails
group u list: _517_
m user id: 0
owner id: 6670
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is the tightly regulated lac operon BBa_K389050 with a mRFP gene as readout.
mutable notes: lacIq integrated in promoter so good regulation, mRFP as readout
mutable provenance: partsregistry
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0034
component: BBa_K389050
component: BBa_E1010
sequence annotation: BBa_K389050
sequence annotation: BBa_B0034
sequence annotation: BBa_E1010
sequence: https://synbiohub.org/public/igem/BBa_K389052_sequence/1

BBa_B0034
type: Component
persistent identity: component2273042
display id: component2273042
version: 1
top level: BBa_K389052
access: public
definition: BBa_B0034

BBa_K389050
type: Component
persistent identity: component2273040
display id: component2273040
version: 1
top level: BBa_K389052
access: public
definition: BBa_K389050

BBa_E1010
type: Component
persistent identity: component2273045
display id: component2273045
version: 1
top level: BBa_K389052
access: public
definition: https://synbiohub.org/public/igem/BBa_E1010/1

BBa_K389050
type: SequenceAnnotation
persistent identity: annotation2273040
display id: annotation2273040
version: 1
top level: BBa_K389052
location: https://synbiohub.org/public/igem/BBa_K389052/annotation2273040/range2273040/1
component: BBa_K389050

https://synbiohub.org/public/igem/BBa_K389052/annotation2273040/range2273040/1
type: Range
persistent identity: range2273040
display id: range2273040
version: 1
direction: direction/reverse
top level: BBa_K389052
start: 1
end: 1600
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2273042
display id: annotation2273042
version: 1
top level: BBa_K389052
location: https://synbiohub.org/public/igem/BBa_K389052/annotation2273042/range2273042/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K389052/annotation2273042/range2273042/1
type: Range
persistent identity: range2273042
display id: range2273042
version: 1
direction: direction/reverse
top level: BBa_K389052
start: 1609
end: 1620
orientation: inline

BBa_E1010
type: SequenceAnnotation
persistent identity: annotation2273045
display id: annotation2273045
version: 1
top level: BBa_K389052
location: https://synbiohub.org/public/igem/BBa_K389052/annotation2273045/range2273045/1
component: BBa_E1010

https://synbiohub.org/public/igem/BBa_K389052/annotation2273045/range2273045/1
type: Range
persistent identity: range2273045
display id: range2273045
version: 1
direction: direction/reverse
top level: BBa_K389052
start: 1627
end: 2332
orientation: inline

BBa_B0036
type: ComponentDefinition
persistent identity: BBa B0036
display id: BBa_B0036
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Specialized RBS
creator: Barry Canton
created: 2004-06-07T11:00:00Z
modified: 2015-08-31T04:07:20Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_6_
m user id: 0
owner id: 135
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This RBS is recognized by ribosomes carrying the modified anti-Shine Dalgarno sequence as found in Brink et al. (see below)
mutable provenance: Brink MF, Verbeet MP, de Boer HA (1995). Specialized ribosomes: highly specific translation in vivo of a single targetted mRNA species. Gene, 156, pp. 215-222
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_B0036_sequence/1

BBa_J61137
type: ComponentDefinition
persistent identity: BBa J61137
display id: BBa_J61137
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Ribosome Binding Site Family Member
creator: John Anderson
created: 2007-04-24T11:00:00Z
modified: 2015-08-31T01:59:49Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: false
mutable description: {{JCA_Arkin_RBSFamily}}
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J61137_sequence/1

pFun
type: ComponentDefinition
persistent identity: BBa K678046
display id: BBa_K678046
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Plasmid-Fun for Plug
creator: DTU-Denmark-2
created: 2011-09-04T11:00:00Z
modified: 2015-05-08T01:13:04Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _882_
m user id: 0
owner id: 8341
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Plasmid for Plug'n'Play. Contains an Ampicillin resistance marker.
mutable notes: Compatible with the Plug'n'Play assembly standard.
mutable provenance: p71
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: pFun
sequence annotation: Linker 6
sequence: https://synbiohub.org/public/igem/BBa_K678046_sequence/1

pFun
type: SequenceAnnotation
persistent identity: annotation2127424
display id: annotation2127424
version: 1
top level: pFun
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K678046/annotation2127424/range2127424/1

https://synbiohub.org/public/igem/BBa_K678046/annotation2127424/range2127424/1
type: Range
persistent identity: range2127424
display id: range2127424
version: 1
direction: direction/forward
top level: pFun
start: 9
end: 2878
orientation: inline

Linker 6
type: SequenceAnnotation
persistent identity: annotation2127423
display id: annotation2127423
version: 1
top level: pFun
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K678046/annotation2127423/range2127423/1

https://synbiohub.org/public/igem/BBa_K678046/annotation2127423/range2127423/1
type: Range
persistent identity: range2127423
display id: range2127423
version: 1
direction: direction/forward
top level: pFun
start: 1
end: 8
orientation: inline

BBa_J64020
type: ComponentDefinition
persistent identity: BBa J64020
display id: BBa_J64020
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: invI RBS
creator: Dan Widmaier
created: 2008-01-16T12:00:00Z
modified: 2015-08-31T01:56:26Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _98_
m user id: 0
owner id: 2407
owning group id: 98
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This part is the natural invI RBS from Salmonella Typhimurium LT2.
mutable notes: This part was not designed with consideration for biobrick standard assembly in mind. This RBS has not been explicitly tested for expression level in a fluorescence or B-Gal assay.
mutable provenance: Salmonella Typhimurium LT2 Genomic DNA
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: invI RBS
sequence: https://synbiohub.org/public/igem/BBa_J64020_sequence/1

invI RBS
type: SequenceAnnotation
persistent identity: annotation1959619
display id: annotation1959619
version: 1
top level: BBa_J64020
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_J64020/annotation1959619/range1959619/1

https://synbiohub.org/public/igem/BBa_J64020/annotation1959619/range1959619/1
type: Range
persistent identity: range1959619
display id: range1959619
version: 1
direction: direction/forward
top level: BBa_J64020
start: 1
end: 20
orientation: inline

R38
type: ComponentDefinition
persistent identity: BBa K780001
display id: BBa_K780001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Strong RBS for Bacillus Subtilis
creator: Krzysztof Szczepaniak
created: 2012-09-25T11:00:00Z
modified: 2015-05-08T01:13:18Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1034_
m user id: 0
owner id: 10271
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Natural RBS sequence for Bacillus Subtilis, described in the literature as a strong RBS for this bacteria.
mutable notes: -
mutable provenance: From genomic sequence Bacillus Subtilis.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_K780001_sequence/1

BBa_K112318
type: ComponentDefinition
persistent identity: BBa K112318
display id: BBa_K112318
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: {< bolA promoter>} in BBb format
creator: Aron Lau
created: 2008-10-20T11:00:00Z
modified: 2015-05-08T01:09:16Z
discontinued: false
dominant: false
group u list: _224_
m user id: 0
owner id: 2999
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: bolA promoter is a promoter where levels of expression are inversely proportional to the growth rate. To read more, go to following link [http://www.pubmedcentral.nih.gov/picrender.fcgi?artid=402084&blobtype=pdf] This part is actually in BBb format. ---- This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at: [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
mutable notes: PCR al0017/al0018 on MG1655 (467bp, EcoRI/BamHI/DpnI) Sub into pBca1256 (EcoRI/BamHI, 2472+910, L) Product is pBca1256-K112318 ----------------------------------------------- al0017 Forward Biobricking of bolA promoter CCATAGAATTCatgAGATCTtgctgtggcagtgtaatcgtc al0018 Reverse Biobricking of bolA promoter CGttaGGATCCcttctatccgctcacgtatc
mutable provenance: MG1655
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K112318_sequence/1

BBa_K112322
type: ComponentDefinition
persistent identity: BBa K112322
display id: BBa_K112322
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: {Pdps} in BBb format
creator: Aron Lau
created: 2008-10-21T11:00:00Z
modified: 2015-05-08T01:09:16Z
discontinued: false
dominant: false
group u list: _224_
m user id: 0
owner id: 2999
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Pdps promoter. This part is actually in BBb format. ---- This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at: [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
mutable notes: PCR JH089F/JH089R on Salmonella L2. (379 bp, EcoRI/BamHI/DpnI) Sub into pBjh1601CK (EcoRI/BamHI, 3134+910, L) Product is pBjh1601CK-K112322 ----------------------------------------------- JH089F Forward biobricking of {Pdps} cgataGAATTCatgAGATCTaacgattcgacgcgaaccgc JH089R Reverse biobricking of {Pdps} cgttaGGATCCaatctcatatcctcttgatg
mutable provenance: Salmonella L2
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K112322_sequence/1

Prm +
type: ComponentDefinition
persistent identity: BBa I12006
display id: BBa_I12006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Modified lamdba Prm promoter (repressed by 434 cI)
creator: mcnamara
created: 2004-07-13T11:00:00Z
modified: 2015-08-31T04:07:31Z
discontinued: false
dominant: false
experience: experience/None
group u list: _3_
m user id: 0
owner id: 147
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Lamdba Prm promoter modified to be activated by lamda repressor (cI) and repressed by 434 repressor (cI)
mutable notes: The O-R1 region of 434 contained 14 base pairs as opposed to the 17 base pairs of the O-R3 site of lambda. Also, it was noticed that the O-R3 site of the lambda included part of the -10 site. Hence, to preserve the spacing and the -10 site, the three nucleotides that were in both the -10 site and the lambda O-R3 site were retained. The 14 nucleotides that were in the O-R3 site and not in the -10 site were replaced with the O-R1 site of the 434.
mutable provenance: Bushman(1993), Shih & Gussin (1983)
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -10
sequence annotation: OR2 lambda
sequence annotation: OR1 lambda
sequence annotation: OR1 434
sequence annotation: -35
sequence: https://synbiohub.org/public/igem/BBa_I12006_sequence/1

-10
type: SequenceAnnotation
persistent identity: annotation837228
display id: annotation837228
version: 1
top level: Prm +
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I12006/annotation837228/range837228/1

https://synbiohub.org/public/igem/BBa_I12006/annotation837228/range837228/1
type: Range
persistent identity: range837228
display id: range837228
version: 1
direction: direction/forward
top level: Prm +
start: 71
end: 76
orientation: inline

OR2 lambda
type: SequenceAnnotation
persistent identity: annotation786518
display id: annotation786518
version: 1
top level: Prm +
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I12006/annotation786518/range786518/1

https://synbiohub.org/public/igem/BBa_I12006/annotation786518/range786518/1
type: Range
persistent identity: range786518
display id: range786518
version: 1
direction: direction/forward
top level: Prm +
start: 33
end: 49
orientation: inline

OR1 lambda
type: SequenceAnnotation
persistent identity: annotation786500
display id: annotation786500
version: 1
top level: Prm +
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I12006/annotation786500/range786500/1

https://synbiohub.org/public/igem/BBa_I12006/annotation786500/range786500/1
type: Range
persistent identity: range786500
display id: range786500
version: 1
direction: direction/forward
top level: Prm +
start: 9
end: 25
orientation: inline

OR1 434
type: SequenceAnnotation
persistent identity: annotation786365
display id: annotation786365
version: 1
top level: Prm +
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I12006/annotation786365/range786365/1

https://synbiohub.org/public/igem/BBa_I12006/annotation786365/range786365/1
type: Range
persistent identity: range786365
display id: range786365
version: 1
direction: direction/forward
top level: Prm +
start: 56
end: 69
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation837284
display id: annotation837284
version: 1
top level: Prm +
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I12006/annotation837284/range837284/1

https://synbiohub.org/public/igem/BBa_I12006/annotation837284/range837284/1
type: Range
persistent identity: range837284
display id: range837284
version: 1
direction: direction/forward
top level: Prm +
start: 48
end: 53
orientation: inline

cmR
type: ComponentDefinition
persistent identity: BBa P1000
display id: BBa_P1000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: chloramphenicol resistance cassette
creator: Austin Che
created: 2005-12-19T12:00:00Z
modified: 2015-05-08T01:14:11Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _41_
m user id: 0
owner id: 22
owning group id: 41
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This provides the entire chloramphenicol resistance operon and will express CmR.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: CmR
sequence: https://synbiohub.org/public/igem/BBa_P1000_sequence/1

CmR
type: SequenceAnnotation
persistent identity: annotation1771290
display id: annotation1771290
version: 1
top level: cmR
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_P1000/annotation1771290/range1771290/1

https://synbiohub.org/public/igem/BBa_P1000/annotation1771290/range1771290/1
type: Range
persistent identity: range1771290
display id: range1771290
version: 1
direction: direction/forward
top level: cmR
start: 107
end: 766
orientation: inline

BBa_J64987
type: ComponentDefinition
persistent identity: BBa J64987
display id: BBa_J64987
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LacI Consensus Binding Site in sigma 70 binding region
creator: Sai Duriseti
created: 2007-03-25T11:00:00Z
modified: 2015-05-08T01:08:18Z
discontinued: false
dominant: false
experience: experience/None
group u list: _98_
m user id: 0
owner id: 1428
owning group id: 98
sample status: Not in stock
status: status/Unavailable
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: LacI
sequence annotation: -35
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_J64987_sequence/1

LacI
type: SequenceAnnotation
persistent identity: annotation1921610
display id: annotation1921610
version: 1
top level: BBa_J64987
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_J64987/annotation1921610/range1921610/1

https://synbiohub.org/public/igem/BBa_J64987/annotation1921610/range1921610/1
type: Range
persistent identity: range1921610
display id: range1921610
version: 1
direction: direction/forward
top level: BBa_J64987
start: 7
end: 26
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1921608
display id: annotation1921608
version: 1
top level: BBa_J64987
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J64987/annotation1921608/range1921608/1

https://synbiohub.org/public/igem/BBa_J64987/annotation1921608/range1921608/1
type: Range
persistent identity: range1921608
display id: range1921608
version: 1
direction: direction/forward
top level: BBa_J64987
start: 1
end: 6
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1921609
display id: annotation1921609
version: 1
top level: BBa_J64987
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J64987/annotation1921609/range1921609/1

https://synbiohub.org/public/igem/BBa_J64987/annotation1921609/range1921609/1
type: Range
persistent identity: range1921609
display id: range1921609
version: 1
direction: direction/forward
top level: BBa_J64987
start: 27
end: 32
orientation: inline

BBa_K090504
type: ComponentDefinition
persistent identity: BBa K090504
display id: BBa_K090504
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Gram-Positive Strong Constitutive Promoter
creator: Daniel Goodman
created: 2008-10-27T12:00:00Z
modified: 2015-05-08T01:08:37Z
discontinued: false
dominant: false
group u list: _188_
m user id: 0
owner id: 3501
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This is a strong constitutive (always-on) promoter for use with gram-positive organisms. It requires a downstream gram-positive ribosomal binding site and coding sequence to function. We only have experience using this part in B. subtilis, but it should function in most gram-positive organisms. It has not been tested and will most likely not work in E. coli. The promoter comes from the cis-regulatory region of the uracil phosphatase in Bacillus cereus. We functionally annotated the promoter ourselves using the DBTBS database motif search feature. (dbtbs.hgc.jp)
mutable notes: This part generates high expression in gram-positive organisms, and it may be possible to generate deleterious effects on the cell if used to express certain protein products.
mutable provenance: We PCRed this part out of the pAD43-25 gram positive vector, acquired from the Bacillus Genetic Stock Center (bgsc.org), catalog number ECE166.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: putative SigA -10
sequence annotation: putative ComK binding region
sequence annotation: putative SigA -35
sequence: https://synbiohub.org/public/igem/BBa_K090504_sequence/1

putative SigA -10
type: SequenceAnnotation
persistent identity: annotation1991957
display id: annotation1991957
version: 1
top level: BBa_K090504
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K090504/annotation1991957/range1991957/1

https://synbiohub.org/public/igem/BBa_K090504/annotation1991957/range1991957/1
type: Range
persistent identity: range1991957
display id: range1991957
version: 1
direction: direction/forward
top level: BBa_K090504
start: 125
end: 130
orientation: inline

putative ComK binding region
type: SequenceAnnotation
persistent identity: annotation1991955
display id: annotation1991955
version: 1
top level: BBa_K090504
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K090504/annotation1991955/range1991955/1

https://synbiohub.org/public/igem/BBa_K090504/annotation1991955/range1991955/1
type: Range
persistent identity: range1991955
display id: range1991955
version: 1
direction: direction/forward
top level: BBa_K090504
start: 76
end: 88
orientation: inline

putative SigA -35
type: SequenceAnnotation
persistent identity: annotation1991956
display id: annotation1991956
version: 1
top level: BBa_K090504
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K090504/annotation1991956/range1991956/1

https://synbiohub.org/public/igem/BBa_K090504/annotation1991956/range1991956/1
type: Range
persistent identity: range1991956
display id: range1991956
version: 1
direction: direction/forward
top level: BBa_K090504
start: 102
end: 108
orientation: inline

gmR
type: ComponentDefinition
persistent identity: BBa P1014
display id: BBa_P1014
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: gentamicin resistance cassette aph(2)
creator: Tom Knight
created: 2006-06-10T11:00:00Z
modified: 2015-05-08T01:14:12Z
discontinued: false
dominant: false
experience: experience/None
group u list: _41_48_1_
m user id: 0
owner id: 6
owning group id: 48
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Novel gentamicin resistance gene. Derived from Genbank DQ208936, pTEX5500ts, kind gift of Barbara E. Murray. This, in turn was derived from Genbank AF016483. See pmid=16391062, pmid=9593155
mutable notes: XbaI site at 660 removed by mutating c->t which also improves codon usage. gatgatttttacagggatattttagagaacgaaatctac,P1014-XF gtagatttcgttctctaaaatatccctgtaaaaatcatc,P1014-XR
mutable provenance: DQ208936
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: stem_loop
sequence annotation: aph(2)
sequence: https://synbiohub.org/public/igem/BBa_P1014_sequence/1

stem_loop
type: SequenceAnnotation
persistent identity: annotation2073661
display id: annotation2073661
version: 1
top level: gmR
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_P1014/annotation2073661/range2073661/1

https://synbiohub.org/public/igem/BBa_P1014/annotation2073661/range2073661/1
type: Range
persistent identity: range2073661
display id: range2073661
version: 1
direction: direction/forward
top level: gmR
start: 1040
end: 1070
orientation: inline

aph(2)
type: SequenceAnnotation
persistent identity: annotation2073660
display id: annotation2073660
version: 1
top level: gmR
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_P1014/annotation2073660/range2073660/1

https://synbiohub.org/public/igem/BBa_P1014/annotation2073660/range2073660/1
type: Range
persistent identity: range2073660
display id: range2073660
version: 1
direction: direction/forward
top level: gmR
start: 120
end: 1025
orientation: inline

BBa_R2110
type: ComponentDefinition
persistent identity: BBa R2110
display id: BBa_R2110
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter with operator site for C2003
creator: Reshma Shetty
created: 2006-10-20T11:00:00Z
modified: 2015-05-08T01:14:16Z
discontinued: false
dominant: false
group u list: _41_
m user id: 0
owner id: 126
owning group id: 70
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This promoter is designed to bind to C2003.
mutable notes: The placement of the operator site was designed to be near optimal for steric interference between C2003 and RNA polymerase.
mutable provenance: This promoter was designed rationally.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: transcription start site
sequence annotation: -35 site
sequence annotation: C2003 operator site
sequence annotation: -10 site
sequence: https://synbiohub.org/public/igem/BBa_R2110_sequence/1

transcription start site
type: SequenceAnnotation
persistent identity: annotation1903918
display id: annotation1903918
version: 1
top level: BBa_R2110
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_R2110/annotation1903918/range1903918/1

https://synbiohub.org/public/igem/BBa_R2110/annotation1903918/range1903918/1
type: Range
persistent identity: range1903918
display id: range1903918
version: 1
direction: direction/forward
top level: BBa_R2110
start: 53
end: 53
orientation: inline

-35 site
type: SequenceAnnotation
persistent identity: annotation1903916
display id: annotation1903916
version: 1
top level: BBa_R2110
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R2110/annotation1903916/range1903916/1

https://synbiohub.org/public/igem/BBa_R2110/annotation1903916/range1903916/1
type: Range
persistent identity: range1903916
display id: range1903916
version: 1
direction: direction/forward
top level: BBa_R2110
start: 17
end: 22
orientation: inline

C2003 operator site
type: SequenceAnnotation
persistent identity: annotation1903919
display id: annotation1903919
version: 1
top level: BBa_R2110
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_R2110/annotation1903919/range1903919/1

https://synbiohub.org/public/igem/BBa_R2110/annotation1903919/range1903919/1
type: Range
persistent identity: range1903919
display id: range1903919
version: 1
direction: direction/forward
top level: BBa_R2110
start: 33
end: 44
orientation: inline

-10 site
type: SequenceAnnotation
persistent identity: annotation1903917
display id: annotation1903917
version: 1
top level: BBa_R2110
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R2110/annotation1903917/range1903917/1

https://synbiohub.org/public/igem/BBa_R2110/annotation1903917/range1903917/1
type: Range
persistent identity: range1903917
display id: range1903917
version: 1
direction: direction/forward
top level: BBa_R2110
start: 40
end: 45
orientation: inline

BBa_B0035
type: ComponentDefinition
persistent identity: BBa B0035
display id: BBa_B0035
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS (B0030 derivative)
creator: Jason Kelly
created: 2004-01-27T12:00:00Z
modified: 2015-08-31T04:07:20Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_B0035_sequence/1

U037O11+D0
type: ComponentDefinition
persistent identity: BBa I732447
display id: BBa_I732447
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Family Member (U037O11+D050O22)
creator: Zhan Jian
created: 2007-10-13T11:00:00Z
modified: 2015-08-31T04:07:58Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732447_sequence/1

BBa_J61124
type: ComponentDefinition
persistent identity: BBa J61124
display id: BBa_J61124
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Ribosome Binding Site Family Member
creator: John Anderson
created: 2007-04-24T11:00:00Z
modified: 2015-08-31T01:59:49Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: false
mutable description: {{JCA_Arkin_RBSFamily}}
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J61124_sequence/1

BBa_K165042
type: ComponentDefinition
persistent identity: BBa K165042
display id: BBa_K165042
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Gli1 binding sites + MET25 inducible yeast promoter
creator: John Szymanski
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:10:56Z
discontinued: false
dominant: false
group u list: _267_
m user id: 0
owner id: 2512
owning group id: 58
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: -
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
component: BBa_K165000
component: BBa_K165012
sequence annotation: BBa_K165012
sequence annotation: BBa_K165000
sequence: https://synbiohub.org/public/igem/BBa_K165042_sequence/1

BBa_K165000
type: Component
persistent identity: component1996563
display id: component1996563
version: 1
top level: BBa_K165042
access: public
definition: BBa_K165000

BBa_K165012
type: Component
persistent identity: component1996562
display id: component1996562
version: 1
top level: BBa_K165042
access: public
definition: https://synbiohub.org/public/igem/BBa_K165012/1

BBa_K165012
type: SequenceAnnotation
persistent identity: annotation1996562
display id: annotation1996562
version: 1
top level: BBa_K165042
location: https://synbiohub.org/public/igem/BBa_K165042/annotation1996562/range1996562/1
component: BBa_K165012

https://synbiohub.org/public/igem/BBa_K165042/annotation1996562/range1996562/1
type: Range
persistent identity: range1996562
display id: range1996562
version: 1
direction: direction/reverse
top level: BBa_K165042
start: 1
end: 127
orientation: inline

BBa_K165000
type: SequenceAnnotation
persistent identity: annotation1996563
display id: annotation1996563
version: 1
top level: BBa_K165042
location: https://synbiohub.org/public/igem/BBa_K165042/annotation1996563/range1996563/1
component: BBa_K165000

https://synbiohub.org/public/igem/BBa_K165042/annotation1996563/range1996563/1
type: Range
persistent identity: range1996563
display id: range1996563
version: 1
direction: direction/reverse
top level: BBa_K165042
start: 136
end: 522
orientation: inline

T22
type: ComponentDefinition
persistent identity: BBa K864501
display id: BBa_K864501
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: T22, P22 late terminator
creator: Erik Gullberg
created: 2012-09-24T11:00:00Z
modified: 2015-05-08T01:13:37Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1124_
m user id: 0
owner id: 10137
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Strong transcriptional terminator consisting of a 20 bp stem-loop from Salmonella phage P22.
mutable notes: Design based on McDowell et al 1994.
mutable provenance: Salmonella phage P22
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_K864501_sequence/1

stem_loop
type: SequenceAnnotation
persistent identity: annotation2197326
display id: annotation2197326
version: 1
top level: T22
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K864501/annotation2197326/range2197326/1

https://synbiohub.org/public/igem/BBa_K864501/annotation2197326/range2197326/1
type: Range
persistent identity: range2197326
display id: range2197326
version: 1
direction: direction/forward
top level: T22
start: 5
end: 34
orientation: inline

U085O11+D0
type: ComponentDefinition
persistent identity: BBa I732421
display id: BBa_I732421
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Family Member (U085O11+D026O22)
creator: Zhan Jian
created: 2007-10-02T11:00:00Z
modified: 2015-08-31T04:07:58Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1592
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Our first NOR logical gate
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732421_sequence/1

BBa_K1585119
type: ComponentDefinition
persistent identity: BBa K1585119
display id: BBa_K1585119
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Anderson Promoter with lacI binding site
creator: Tobias Schwanemann
created: 2015-08-31T11:00:00Z
modified: 2015-09-01T02:33:36Z
discontinued: false
dominant: false
experience: experience/Fails
group u list: _2002_
m user id: 24474
owner id: 24474
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Promoters of the Anderson Promoter Collection with lacI binding site derived from [http://parts.igem.org/Part:BBa_R0010 R_0010] to make them [http://openwetware.org/wiki/IPTG IPTG] iducible.
mutable notes: We choosed lacI-binding-site of part BBa_R0010 because we assumed older parts would be more reliable. The part is shipped in backbone [http://parts.igem.org/Part:BBa_J61002 J61002] because the functionality is directly visible by adding IPTG. RFP ([http://parts.igem.org/Part:BBa_E1010 E1010]) should get expressed then.
mutable provenance: Assambly of [http://parts.igem.org/Part:BBa_J23119 J23119] and lacI-binding-site of [http://parts.igem.org/Part:BBa_R0010 R_0010] The "lacI binding site" of Part [http://parts.igem.org/Part:BBa_R0010 R_0010] differs from lacO from Part [http://parts.igem.org/Part:BBa_J33207 BBa_J33207].
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
component: BBa_J23119
component: BBa_K1585999
sequence annotation: BBa_K1585999
sequence annotation: BBa_J23119
sequence: https://synbiohub.org/public/igem/BBa_K1585119_sequence/1

BBa_J23119
type: Component
persistent identity: component2441719
display id: component2441719
version: 1
top level: BBa_K1585119
access: public
definition: BBa_J23119

BBa_K1585999
type: Component
persistent identity: component2441721
display id: component2441721
version: 1
top level: BBa_K1585119
access: public
definition: https://synbiohub.org/public/igem/BBa_K1585999/1

BBa_K1585999
type: SequenceAnnotation
persistent identity: annotation2441721
display id: annotation2441721
version: 1
top level: BBa_K1585119
location: https://synbiohub.org/public/igem/BBa_K1585119/annotation2441721/range2441721/1
component: BBa_K1585999

https://synbiohub.org/public/igem/BBa_K1585119/annotation2441721/range2441721/1
type: Range
persistent identity: range2441721
display id: range2441721
version: 1
direction: direction/reverse
top level: BBa_K1585119
start: 44
end: 78
orientation: inline

BBa_J23119
type: SequenceAnnotation
persistent identity: annotation2441719
display id: annotation2441719
version: 1
top level: BBa_K1585119
location: https://synbiohub.org/public/igem/BBa_K1585119/annotation2441719/range2441719/1
component: BBa_J23119

https://synbiohub.org/public/igem/BBa_K1585119/annotation2441719/range2441719/1
type: Range
persistent identity: range2441719
display id: range2441719
version: 1
direction: direction/reverse
top level: BBa_K1585119
start: 1
end: 35
orientation: inline

BBa_J61127
type: ComponentDefinition
persistent identity: BBa J61127
display id: BBa_J61127
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Ribosome Binding Site Family Member
creator: John Anderson
created: 2007-04-24T11:00:00Z
modified: 2015-08-31T01:59:49Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: false
mutable description: {{JCA_Arkin_RBSFamily}}
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J61127_sequence/1

BBa_R0181
type: ComponentDefinition
persistent identity: BBa R0181
display id: BBa_R0181
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: T7 RNAP promoter
creator: Barry Canton
created: 2005-09-25T11:00:00Z
modified: 2015-05-08T01:14:15Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_6_
m user id: 0
owner id: 135
owning group id: 6
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: T7 promoter with an T->G mutation at the -17 position of the consensus sequence
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_R0181_sequence/1

BBa_K284003
type: ComponentDefinition
persistent identity: BBa K284003
display id: BBa_K284003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Partial DLD Promoter from Kluyveromyces lactis
creator: Tais Sineiro Herig, Raissa Estrela Curado and Gleidson Silva Teixeira
created: 2009-09-25T11:00:00Z
modified: 2015-05-08T01:11:48Z
discontinued: false
dominant: false
experience: experience/None
group u list: _386_
m user id: 0
owner id: 4968
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: .
mutable notes: .
mutable provenance: .
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: primer_binding
sequence annotation: DLD Promoter
sequence annotation: TATA-Like
sequence: https://synbiohub.org/public/igem/BBa_K284003_sequence/1

primer_binding
type: SequenceAnnotation
persistent identity: annotation2034973
display id: annotation2034973
version: 1
top level: BBa_K284003
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K284003/annotation2034973/range2034973/1

https://synbiohub.org/public/igem/BBa_K284003/annotation2034973/range2034973/1
type: Range
persistent identity: range2034973
display id: range2034973
version: 1
direction: direction/forward
top level: BBa_K284003
start: 1
end: 22
orientation: inline

primer_binding
type: SequenceAnnotation
persistent identity: annotation2034976
display id: annotation2034976
version: 1
top level: BBa_K284003
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K284003/annotation2034976/range2034976/1

https://synbiohub.org/public/igem/BBa_K284003/annotation2034976/range2034976/1
type: Range
persistent identity: range2034976
display id: range2034976
version: 1
direction: direction/reverse
top level: BBa_K284003
start: 442
end: 463
orientation: inline

DLD Promoter
type: SequenceAnnotation
persistent identity: annotation2034972
display id: annotation2034972
version: 1
top level: BBa_K284003
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K284003/annotation2034972/range2034972/1

https://synbiohub.org/public/igem/BBa_K284003/annotation2034972/range2034972/1
type: Range
persistent identity: range2034972
display id: range2034972
version: 1
direction: direction/forward
top level: BBa_K284003
start: 1
end: 463
orientation: inline

TATA-Like
type: SequenceAnnotation
persistent identity: annotation2034975
display id: annotation2034975
version: 1
top level: BBa_K284003
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K284003/annotation2034975/range2034975/1

https://synbiohub.org/public/igem/BBa_K284003/annotation2034975/range2034975/1
type: Range
persistent identity: range2034975
display id: range2034975
version: 1
direction: direction/forward
top level: BBa_K284003
start: 355
end: 372
orientation: inline

TATA-Like
type: SequenceAnnotation
persistent identity: annotation2034974
display id: annotation2034974
version: 1
top level: BBa_K284003
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K284003/annotation2034974/range2034974/1

https://synbiohub.org/public/igem/BBa_K284003/annotation2034974/range2034974/1
type: Range
persistent identity: range2034974
display id: range2034974
version: 1
direction: direction/forward
top level: BBa_K284003
start: 300
end: 330
orientation: inline

cmR
type: ComponentDefinition
persistent identity: BBa P1004
display id: BBa_P1004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: chloramphenicol resistance cassette
creator: Reshma Shetty
created: 2006-02-03T12:00:00Z
modified: 2015-05-08T01:14:11Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _41_6_
m user id: 0
owner id: 126
owning group id: 45
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: same part as BBa_P1002 except in reverse orientation.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: cmR
sequence: https://synbiohub.org/public/igem/BBa_P1004_sequence/1

cmR
type: SequenceAnnotation
persistent identity: annotation1897114
display id: annotation1897114
version: 1
top level: cmR
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_P1004/annotation1897114/range1897114/1

https://synbiohub.org/public/igem/BBa_P1004/annotation1897114/range1897114/1
type: Range
persistent identity: range1897114
display id: range1897114
version: 1
direction: direction/forward
top level: cmR
start: 107
end: 769
orientation: inline

BBa_J23104
type: ComponentDefinition
persistent identity: BBa J23104
display id: BBa_J23104
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: constitutive promoter family member
creator: John Anderson
created: 2006-08-03T11:00:00Z
modified: 2015-08-31T04:08:40Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: true
mutable description: replace later
mutable notes: N/A
mutable provenance: isolated from library of promoters
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J23104_sequence/1

cII p22
type: ComponentDefinition
persistent identity: BBa R0053
display id: BBa_R0053
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter (p22 cII regulated)
creator: Maia Mahoney
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: The p22 cII regulatory region sequence is a 97 base-pair sequence with the standard BioBrick prefix and suffix sections on its ends. p22 cII repressor protein, BBa_C0053, binds to it. This segment contains O-R1, O-R2, a fragment of O-R3, the -35 of P-RM, and P-R (-10 and -35 from Tom Knight)
mutable provenance: Bacteriophage p22.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: OR1
sequence annotation: OR3
sequence annotation: BBa_R0053
sequence annotation: OR2
sequence annotation: -10
sequence annotation: -35
sequence: https://synbiohub.org/public/igem/BBa_R0053_sequence/1

OR1
type: SequenceAnnotation
persistent identity: annotation2037
display id: annotation2037
version: 1
top level: cII p22
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0053/annotation2037/range2037/1

https://synbiohub.org/public/igem/BBa_R0053/annotation2037/range2037/1
type: Range
persistent identity: range2037
display id: range2037
version: 1
direction: direction/forward
top level: cII p22
start: 34
end: 51
orientation: inline

OR3
type: SequenceAnnotation
persistent identity: annotation2035
display id: annotation2035
version: 1
top level: cII p22
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0053/annotation2035/range2035/1

https://synbiohub.org/public/igem/BBa_R0053/annotation2035/range2035/1
type: Range
persistent identity: range2035
display id: range2035
version: 1
direction: direction/forward
top level: cII p22
start: 1
end: 3
orientation: inline

BBa_R0053
type: SequenceAnnotation
persistent identity: annotation7069
display id: annotation7069
version: 1
top level: cII p22
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_R0053/annotation7069/range7069/1

https://synbiohub.org/public/igem/BBa_R0053/annotation7069/range7069/1
type: Range
persistent identity: range7069
display id: range7069
version: 1
direction: direction/forward
top level: cII p22
start: 1
end: 54
orientation: inline

OR2
type: SequenceAnnotation
persistent identity: annotation2036
display id: annotation2036
version: 1
top level: cII p22
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0053/annotation2036/range2036/1

https://synbiohub.org/public/igem/BBa_R0053/annotation2036/range2036/1
type: Range
persistent identity: range2036
display id: range2036
version: 1
direction: direction/forward
top level: cII p22
start: 11
end: 28
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2042
display id: annotation2042
version: 1
top level: cII p22
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0053/annotation2042/range2042/1

https://synbiohub.org/public/igem/BBa_R0053/annotation2042/range2042/1
type: Range
persistent identity: range2042
display id: range2042
version: 1
direction: direction/forward
top level: cII p22
start: 30
end: 35
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2038
display id: annotation2038
version: 1
top level: cII p22
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0053/annotation2038/range2038/1

https://synbiohub.org/public/igem/BBa_R0053/annotation2038/range2038/1
type: Range
persistent identity: range2038
display id: range2038
version: 1
direction: direction/forward
top level: cII p22
start: 18
end: 23
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2041
display id: annotation2041
version: 1
top level: cII p22
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0053/annotation2041/range2041/1

https://synbiohub.org/public/igem/BBa_R0053/annotation2041/range2041/1
type: Range
persistent identity: range2041
display id: range2041
version: 1
direction: direction/forward
top level: cII p22
start: 8
end: 13
orientation: inline

cI 434
type: ComponentDefinition
persistent identity: BBa R0052
display id: BBa_R0052
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter (434 cI regulated)
creator: Maia Mahoney
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
discontinued: false
dominant: false
experience: experience/Issues
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: The 434 cI regulatory region sequence is a 89 base-pair sequence with the standard BioBrick prefix and suffix sections on its ends. 434 cI repressor protein, <bb_part>BBa_C0052<bb_part>, binds to it. This segment contains O-R1, O-R2, P-R, and P-RM -35.
mutable provenance: Bacteriophage 434 right operator
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -35
sequence annotation: OR2
sequence annotation: start
sequence annotation: OR1
sequence annotation: -10
sequence annotation: BBa_R0052
sequence: https://synbiohub.org/public/igem/BBa_R0052_sequence/1

-35
type: SequenceAnnotation
persistent identity: annotation2029
display id: annotation2029
version: 1
top level: cI 434
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0052/annotation2029/range2029/1

https://synbiohub.org/public/igem/BBa_R0052/annotation2029/range2029/1
type: Range
persistent identity: range2029
display id: range2029
version: 1
direction: direction/forward
top level: cI 434
start: 1
end: 6
orientation: inline

OR2
type: SequenceAnnotation
persistent identity: annotation2027
display id: annotation2027
version: 1
top level: cI 434
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0052/annotation2027/range2027/1

https://synbiohub.org/public/igem/BBa_R0052/annotation2027/range2027/1
type: Range
persistent identity: range2027
display id: range2027
version: 1
direction: direction/forward
top level: cI 434
start: 8
end: 21
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2032
display id: annotation2032
version: 1
top level: cI 434
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_R0052/annotation2032/range2032/1

https://synbiohub.org/public/igem/BBa_R0052/annotation2032/range2032/1
type: Range
persistent identity: range2032
display id: range2032
version: 1
direction: direction/forward
top level: cI 434
start: 35
end: 37
orientation: inline

OR2
type: SequenceAnnotation
persistent identity: annotation2026
display id: annotation2026
version: 1
top level: cI 434
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0052/annotation2026/range2026/1

https://synbiohub.org/public/igem/BBa_R0052/annotation2026/range2026/1
type: Range
persistent identity: range2026
display id: range2026
version: 1
direction: direction/forward
top level: cI 434
start: 1
end: 43
orientation: inline

OR1
type: SequenceAnnotation
persistent identity: annotation2028
display id: annotation2028
version: 1
top level: cI 434
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0052/annotation2028/range2028/1

https://synbiohub.org/public/igem/BBa_R0052/annotation2028/range2028/1
type: Range
persistent identity: range2028
display id: range2028
version: 1
direction: direction/forward
top level: cI 434
start: 30
end: 43
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2031
display id: annotation2031
version: 1
top level: cI 434
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0052/annotation2031/range2031/1

https://synbiohub.org/public/igem/BBa_R0052/annotation2031/range2031/1
type: Range
persistent identity: range2031
display id: range2031
version: 1
direction: direction/forward
top level: cI 434
start: 2
end: 7
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2030
display id: annotation2030
version: 1
top level: cI 434
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0052/annotation2030/range2030/1

https://synbiohub.org/public/igem/BBa_R0052/annotation2030/range2030/1
type: Range
persistent identity: range2030
display id: range2030
version: 1
direction: direction/forward
top level: cI 434
start: 24
end: 29
orientation: inline

BBa_R0052
type: SequenceAnnotation
persistent identity: annotation7068
display id: annotation7068
version: 1
top level: cI 434
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_R0052/annotation7068/range7068/1

https://synbiohub.org/public/igem/BBa_R0052/annotation7068/range7068/1
type: Range
persistent identity: range7068
display id: range7068
version: 1
direction: direction/forward
top level: cI 434
start: 1
end: 46
orientation: inline

BBa_I51003
type: ComponentDefinition
persistent identity: BBa I51003
display id: BBa_I51003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: -- No description --
creator: Drew Endy
created: 2005-12-01T12:00:00Z
modified: 2015-08-31T04:07:42Z
discontinued: false
dominant: false
group u list: _47_
m user id: 0
owner id: 52
owning group id: 47
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Super duper part!
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: foo binding site
sequence: https://synbiohub.org/public/igem/BBa_I51003_sequence/1

foo binding site
type: SequenceAnnotation
persistent identity: annotation1756359
display id: annotation1756359
version: 1
top level: BBa_I51003
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I51003/annotation1756359/range1756359/1

https://synbiohub.org/public/igem/BBa_I51003/annotation1756359/range1756359/1
type: Range
persistent identity: range1756359
display id: range1756359
version: 1
direction: direction/forward
top level: BBa_I51003
start: 10
end: 50
orientation: inline

mCherry-TS
type: ComponentDefinition
persistent identity: BBa K678014
display id: BBa_K678014
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: mCherry-TS
creator: DTU-Danmark-2
created: 2011-09-12T11:00:00Z
modified: 2015-05-08T01:13:04Z
discontinued: false
dominant: false
experience: experience/None
group u list: _882_
m user id: 0
owner id: 10556
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part was designed for the Plug'n'Play with DNA assembly standard (RFC 80), therefore this part contains tails at the 5' and 3' ends as shown on the figure below.
mutable notes: No
mutable provenance: The Danish Canser Society
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: Linker 3
sequence annotation: mCherry-TS
sequence annotation: misc
sequence: https://synbiohub.org/public/igem/BBa_K678014_sequence/1

Linker 3
type: SequenceAnnotation
persistent identity: annotation2127756
display id: annotation2127756
version: 1
top level: mCherry-TS
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K678014/annotation2127756/range2127756/1

https://synbiohub.org/public/igem/BBa_K678014/annotation2127756/range2127756/1
type: Range
persistent identity: range2127756
display id: range2127756
version: 1
direction: direction/forward
top level: mCherry-TS
start: 1
end: 9
orientation: inline

mCherry-TS
type: SequenceAnnotation
persistent identity: annotation2127757
display id: annotation2127757
version: 1
top level: mCherry-TS
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K678014/annotation2127757/range2127757/1

https://synbiohub.org/public/igem/BBa_K678014/annotation2127757/range2127757/1
type: Range
persistent identity: range2127757
display id: range2127757
version: 1
direction: direction/forward
top level: mCherry-TS
start: 17
end: 727
orientation: inline

misc
type: SequenceAnnotation
persistent identity: annotation2127780
display id: annotation2127780
version: 1
top level: mCherry-TS
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K678014/annotation2127780/range2127780/1

https://synbiohub.org/public/igem/BBa_K678014/annotation2127780/range2127780/1
type: Range
persistent identity: range2127780
display id: range2127780
version: 1
direction: direction/forward
top level: mCherry-TS
start: 10
end: 20
orientation: inline

BBa_K1585105
type: ComponentDefinition
persistent identity: BBa K1585105
display id: BBa_K1585105
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Anderson Promoter with lacI binding site
creator: Tobias Schwanemann
created: 2015-08-31T11:00:00Z
modified: 2015-09-01T02:03:04Z
discontinued: false
dominant: false
experience: experience/Fails
group u list: _2002_
m user id: 24474
owner id: 24474
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Promoters of the Anderson Promoter Collection with lacI binding site derived from [http://parts.igem.org/Part:BBa_R0010 R_0010] to make them [http://openwetware.org/wiki/IPTG IPTG] iducible.
mutable notes: We choosed lacI-binding-site of part BBa_R0010 because we assumed older parts would be more reliable. The part is shipped in backbone [http://parts.igem.org/Part:BBa_J61002 J61002] because the functionality is directly visible by adding IPTG. RFP ([http://parts.igem.org/Part:BBa_E1010 E1010]) should get expressed then.
mutable provenance: Assambly of [http://parts.igem.org/Part:BBa_J23103 J23103] and lacI-binding-site of [http://parts.igem.org/Part:BBa_R0010 R_0010] The "lacI binding site" of Part [http://parts.igem.org/Part:BBa_R0010 R_0010] differs from lacO from Part [http://parts.igem.org/Part:BBa_J33207 BBa_J33207].
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
component: BBa_K1585999
component: BBa_J23105
sequence annotation: BBa_J23105
sequence annotation: BBa_K1585999
sequence: https://synbiohub.org/public/igem/BBa_K1585105_sequence/1

BBa_K1585999
type: Component
persistent identity: component2441416
display id: component2441416
version: 1
top level: BBa_K1585105
access: public
definition: https://synbiohub.org/public/igem/BBa_K1585999/1

BBa_J23105
type: Component
persistent identity: component2441414
display id: component2441414
version: 1
top level: BBa_K1585105
access: public
definition: BBa_J23105

BBa_J23105
type: SequenceAnnotation
persistent identity: annotation2441414
display id: annotation2441414
version: 1
top level: BBa_K1585105
location: https://synbiohub.org/public/igem/BBa_K1585105/annotation2441414/range2441414/1
component: BBa_J23105

https://synbiohub.org/public/igem/BBa_K1585105/annotation2441414/range2441414/1
type: Range
persistent identity: range2441414
display id: range2441414
version: 1
direction: direction/reverse
top level: BBa_K1585105
start: 1
end: 35
orientation: inline

BBa_K1585999
type: SequenceAnnotation
persistent identity: annotation2441416
display id: annotation2441416
version: 1
top level: BBa_K1585105
location: https://synbiohub.org/public/igem/BBa_K1585105/annotation2441416/range2441416/1
component: BBa_K1585999

https://synbiohub.org/public/igem/BBa_K1585105/annotation2441416/range2441416/1
type: Range
persistent identity: range2441416
display id: range2441416
version: 1
direction: direction/reverse
top level: BBa_K1585105
start: 44
end: 78
orientation: inline

BBa_J23111
type: ComponentDefinition
persistent identity: BBa J23111
display id: BBa_J23111
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: constitutive promoter family member
creator: John Anderson
created: 2006-08-16T11:00:00Z
modified: 2015-08-31T04:08:40Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: true
mutable description: Later
mutable notes: N/A
mutable provenance: Later
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J23111_sequence/1

pMam
type: ComponentDefinition
persistent identity: BBa K678023
display id: BBa_K678023
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Plasmid-Mam for Plug
creator: DTU-Denmark-2
created: 2011-09-04T11:00:00Z
modified: 2015-05-08T01:13:04Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _882_
m user id: 0
owner id: 8341
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Plasmid-Mam for Plug'n'Play
mutable notes: Compatible with the Plug'n'Play assembly standard
mutable provenance: PU0020
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: Linker
sequence annotation: Plasmid Mam
sequence: https://synbiohub.org/public/igem/BBa_K678023_sequence/1

Linker
type: SequenceAnnotation
persistent identity: annotation2127360
display id: annotation2127360
version: 1
top level: pMam
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K678023/annotation2127360/range2127360/1

https://synbiohub.org/public/igem/BBa_K678023/annotation2127360/range2127360/1
type: Range
persistent identity: range2127360
display id: range2127360
version: 1
direction: direction/forward
top level: pMam
start: 1
end: 8
orientation: inline

Plasmid Mam
type: SequenceAnnotation
persistent identity: annotation2127361
display id: annotation2127361
version: 1
top level: pMam
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K678023/annotation2127361/range2127361/1

https://synbiohub.org/public/igem/BBa_K678023/annotation2127361/range2127361/1
type: Range
persistent identity: range2127361
display id: range2127361
version: 1
direction: direction/forward
top level: pMam
start: 9
end: 2711
orientation: inline

BBa_M13110
type: ComponentDefinition
persistent identity: BBa M13110
display id: BBa_M13110
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: M13110
creator: Natalie Kuldell
created: 2006-12-15T12:00:00Z
modified: 2015-05-08T01:13:55Z
discontinued: false
dominant: false
group u list: _45_
m user id: 0
owner id: 314
owning group id: 1
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This part is from the bacteriophage M13 genome, bp 381-428 in M13K07. It directs transcription of M13 gene X (BBa_M13510, BBa_M13010). Its identity as a promoter was described in Gene (1980) 11:129-148 based on -10,-35 homology and fragment of genome able to bind RNAP.
mutable notes: none
mutable provenance: New England Biolabs M13K07. Sequenced in 2002 by L. Panganaban.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_M13110_sequence/1

R37
type: ComponentDefinition
persistent identity: BBa K780002
display id: BBa_K780002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Strong RBS for Bacillus Subtilis
creator: Krzysztof Szczepaniak
created: 2012-09-25T11:00:00Z
modified: 2015-05-08T01:13:18Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1034_
m user id: 0
owner id: 10113
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Strong RBS for Bacillus Subtilis
mutable notes: -
mutable provenance: 'host-takover module' from B.subtilis Bacteriophage SPO1
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_K780002_sequence/1

BBa_K112400
type: ComponentDefinition
persistent identity: BBa K112400
display id: BBa_K112400
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter for grpE gene - Heat Shock and Ultrasound Sensitive
creator: Christina Brown
created: 2008-10-19T11:00:00Z
modified: 2015-05-08T01:09:17Z
discontinued: false
dominant: true
group u list: _224_
m user id: 0
owner id: 3241
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: According to Vollmer, et. al in their 1998 Applied and Enviormental Microbiology paper, "Bacterial Stress Responses to 1-Megahertz Pulsed Ultrasound in the Presence of Microbubble", the promoter for the grpE gene, a known heat shock promoter, is upregulated 24 times when cells are exposed to high frequency ultrasound and microbubble cavitation.
mutable notes: none
mutable provenance: cloned from E. coli MG1655 genome
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K112400_sequence/1

BBa_I739103
type: ComponentDefinition
persistent identity: BBa I739103
display id: BBa_I739103
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Double Promoter (lacI, negative / P22 cII, negative)
creator: Stefan Luzi
created: 2007-10-14T11:00:00Z
modified: 2015-08-31T04:08:01Z
discontinued: false
dominant: false
group u list: _124_
m user id: 0
owner id: 2183
owning group id: 62
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: asdf
mutable notes: asdf
mutable provenance: asf
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: OR1
sequence annotation: R0011
sequence annotation: R0053 (2x OR1)
sequence: https://synbiohub.org/public/igem/BBa_I739103_sequence/1

OR1
type: SequenceAnnotation
persistent identity: annotation1947822
display id: annotation1947822
version: 1
top level: BBa_I739103
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I739103/annotation1947822/range1947822/1

https://synbiohub.org/public/igem/BBa_I739103/annotation1947822/range1947822/1
type: Range
persistent identity: range1947822
display id: range1947822
version: 1
direction: direction/forward
top level: BBa_I739103
start: 69
end: 87
orientation: inline

OR1
type: SequenceAnnotation
persistent identity: annotation1947821
display id: annotation1947821
version: 1
top level: BBa_I739103
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I739103/annotation1947821/range1947821/1

https://synbiohub.org/public/igem/BBa_I739103/annotation1947821/range1947821/1
type: Range
persistent identity: range1947821
display id: range1947821
version: 1
direction: direction/forward
top level: BBa_I739103
start: 50
end: 68
orientation: inline

R0011
type: SequenceAnnotation
persistent identity: annotation1947820
display id: annotation1947820
version: 1
top level: BBa_I739103
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I739103/annotation1947820/range1947820/1

https://synbiohub.org/public/igem/BBa_I739103/annotation1947820/range1947820/1
type: Range
persistent identity: range1947820
display id: range1947820
version: 1
direction: direction/forward
top level: BBa_I739103
start: 1
end: 48
orientation: inline

R0053 (2x OR1)
type: SequenceAnnotation
persistent identity: annotation1947823
display id: annotation1947823
version: 1
top level: BBa_I739103
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_I739103/annotation1947823/range1947823/1

https://synbiohub.org/public/igem/BBa_I739103/annotation1947823/range1947823/1
type: Range
persistent identity: range1947823
display id: range1947823
version: 1
direction: direction/forward
top level: BBa_I739103
start: 50
end: 87
orientation: inline

MTS
type: ComponentDefinition
persistent identity: BBa K678025
display id: BBa_K678025
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Mitochondrial targeting sequence for Aspergillus nidulans
creator: DTU-Denmark-2
created: 2011-09-03T11:00:00Z
modified: 2015-05-08T01:13:04Z
discontinued: false
dominant: false
experience: experience/None
group u list: _882_
m user id: 0
owner id: 8341
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Mitochondrial targeting sequence (MTS) for Aspergillus nidulans. A MTS is usually required for the entry of proteins and intermediary metabolites into mitochondria. The MTS is a DNA fragment encoding the N-terminal 60 amino acids of the AcuJ.
mutable notes: Compatible with the Plug'n'Play assembly standard. This MTS should be placed at the N-terminal of the gene of interest.
mutable provenance: Aspergillus nidulans genomic DNA (AN6279)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Other
role: SO:0000110
sequence annotation: Linker 2
sequence annotation: MTS
sequence: https://synbiohub.org/public/igem/BBa_K678025_sequence/1

Linker 2
type: SequenceAnnotation
persistent identity: annotation2127379
display id: annotation2127379
version: 1
top level: MTS
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K678025/annotation2127379/range2127379/1

https://synbiohub.org/public/igem/BBa_K678025/annotation2127379/range2127379/1
type: Range
persistent identity: range2127379
display id: range2127379
version: 1
direction: direction/forward
top level: MTS
start: 1
end: 8
orientation: inline

MTS
type: SequenceAnnotation
persistent identity: annotation2125850
display id: annotation2125850
version: 1
top level: MTS
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K678025/annotation2125850/range2125850/1

https://synbiohub.org/public/igem/BBa_K678025/annotation2125850/range2125850/1
type: Range
persistent identity: range2125850
display id: range2125850
version: 1
direction: direction/forward
top level: MTS
start: 9
end: 188
orientation: inline

RBS
type: ComponentDefinition
persistent identity: BBa J29048
display id: BBa_J29048
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS
creator: Maiko Furubayashi
created: 2006-10-19T11:00:00Z
modified: 2015-08-31T04:08:45Z
discontinued: false
dominant: false
experience: experience/None
group u list: _59_
m user id: 0
owner id: 846
owning group id: 59
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: strong rbs
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J29048_sequence/1

BBa_J23115
type: ComponentDefinition
persistent identity: BBa J23115
display id: BBa_J23115
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: constitutive promoter family member
creator: John Anderson
created: 2006-08-16T11:00:00Z
modified: 2015-08-31T04:08:40Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: true
mutable description: Later
mutable notes: N/A
mutable provenance: Later
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J23115_sequence/1

U073O11+D0
type: ComponentDefinition
persistent identity: BBa I732425
display id: BBa_I732425
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Family Member (U073O11+D002O22)
creator: Zhan Jian
created: 2007-10-13T11:00:00Z
modified: 2015-08-31T04:07:58Z
discontinued: false
dominant: true
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732425_sequence/1

BBa_J23151
type: ComponentDefinition
persistent identity: BBa J23151
display id: BBa_J23151
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: 1bp mutant from J23114
creator: Jason Kelly
created: 2008-02-11T12:00:00Z
modified: 2015-08-31T04:08:41Z
discontinued: false
dominant: false
group u list: _11_
m user id: 0
owner id: 2
owning group id: 84
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: .
mutable notes: .
mutable provenance: .
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J23151_sequence/1

BBa_K1585102
type: ComponentDefinition
persistent identity: BBa K1585102
display id: BBa_K1585102
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Anderson Promoter with lacI binding site
creator: Tobias Schwanemann
created: 2015-08-31T11:00:00Z
modified: 2015-09-01T02:03:57Z
discontinued: false
dominant: false
experience: experience/Fails
group u list: _2002_
m user id: 24474
owner id: 24474
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Promoters of the Anderson Promoter Collection with lacI binding site derived from [http://parts.igem.org/Part:BBa_R0010 R_0010] to make them [http://openwetware.org/wiki/IPTG IPTG] iducible.
mutable notes: We choosed lacI-binding-site of part BBa_R0010 because we assumed older parts would be more reliable. The part is shipped in backbone [http://parts.igem.org/Part:BBa_J61002 J61002] because the functionality is directly visible by adding IPTG. RFP ([http://parts.igem.org/Part:BBa_E1010 E1010]) should get expressed then.
mutable provenance: Assambly of [http://parts.igem.org/Part:BBa_J23102 J23102] and lacI-binding-site of [http://parts.igem.org/Part:BBa_R0010 R_0010] The "lacI binding site" of Part [http://parts.igem.org/Part:BBa_R0010 R_0010] differs from lacO from Part [http://parts.igem.org/Part:BBa_J33207 BBa_J33207].
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
component: BBa_J23102
component: BBa_K1585999
sequence annotation: BBa_J23102
sequence annotation: BBa_K1585999
sequence: https://synbiohub.org/public/igem/BBa_K1585102_sequence/1

BBa_J23102
type: Component
persistent identity: component2441417
display id: component2441417
version: 1
top level: BBa_K1585102
access: public
definition: BBa_J23102

BBa_K1585999
type: Component
persistent identity: component2441419
display id: component2441419
version: 1
top level: BBa_K1585102
access: public
definition: https://synbiohub.org/public/igem/BBa_K1585999/1

BBa_J23102
type: SequenceAnnotation
persistent identity: annotation2441417
display id: annotation2441417
version: 1
top level: BBa_K1585102
location: https://synbiohub.org/public/igem/BBa_K1585102/annotation2441417/range2441417/1
component: BBa_J23102

https://synbiohub.org/public/igem/BBa_K1585102/annotation2441417/range2441417/1
type: Range
persistent identity: range2441417
display id: range2441417
version: 1
direction: direction/reverse
top level: BBa_K1585102
start: 1
end: 35
orientation: inline

BBa_K1585999
type: SequenceAnnotation
persistent identity: annotation2441419
display id: annotation2441419
version: 1
top level: BBa_K1585102
location: https://synbiohub.org/public/igem/BBa_K1585102/annotation2441419/range2441419/1
component: BBa_K1585999

https://synbiohub.org/public/igem/BBa_K1585102/annotation2441419/range2441419/1
type: Range
persistent identity: range2441419
display id: range2441419
version: 1
direction: direction/reverse
top level: BBa_K1585102
start: 44
end: 78
orientation: inline

lux pR-3
type: ComponentDefinition
persistent identity: BBa K658006
display id: BBa_K658006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: position 3 mutated promoter lux pR-3 (luxR & HSL regulated)
creator: Yidan Hu
created: 2011-09-30T11:00:00Z
modified: 2015-05-08T01:13:02Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _835_
m user id: 0
owner id: 10207
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: The position 3 mutated promoter lux pR-3 is a product of site-directed mutagenesis of promoter lux pR (BBa_R0062). The promoter lux pR is based on the Vibrio fischeri quorum sensing gene promoters. The position 3 mutated promoter lux pR-3 gives a higher expression of downstream genes in an autoinduction system. In another word, the lux pR-3 has a higher efficiency than the lux pR. Similar to promoter lux pR, the expression is up-regulated by the action of LuxR protein combined with the signal molecule HSL.
mutable notes: In the promoter lux pR's sequence, the C residue of position 3 was changed to a T using PCR method.
mutable provenance: The position 3 mutated promoter lux pR-3 is based on promoter lux pR (BBa_R0062).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -35
sequence annotation: -10
sequence annotation: K658006
sequence annotation: start
sequence annotation: LuxR/HSL
sequence: https://synbiohub.org/public/igem/BBa_K658006_sequence/1

-35
type: SequenceAnnotation
persistent identity: annotation2148161
display id: annotation2148161
version: 1
top level: lux pR-3
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K658006/annotation2148161/range2148161/1

https://synbiohub.org/public/igem/BBa_K658006/annotation2148161/range2148161/1
type: Range
persistent identity: range2148161
display id: range2148161
version: 1
direction: direction/forward
top level: lux pR-3
start: 20
end: 25
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2148162
display id: annotation2148162
version: 1
top level: lux pR-3
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K658006/annotation2148162/range2148162/1

https://synbiohub.org/public/igem/BBa_K658006/annotation2148162/range2148162/1
type: Range
persistent identity: range2148162
display id: range2148162
version: 1
direction: direction/forward
top level: lux pR-3
start: 42
end: 47
orientation: inline

K658006
type: SequenceAnnotation
persistent identity: annotation2148164
display id: annotation2148164
version: 1
top level: lux pR-3
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K658006/annotation2148164/range2148164/1

https://synbiohub.org/public/igem/BBa_K658006/annotation2148164/range2148164/1
type: Range
persistent identity: range2148164
display id: range2148164
version: 1
direction: direction/forward
top level: lux pR-3
start: 1
end: 55
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2148163
display id: annotation2148163
version: 1
top level: lux pR-3
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K658006/annotation2148163/range2148163/1

https://synbiohub.org/public/igem/BBa_K658006/annotation2148163/range2148163/1
type: Range
persistent identity: range2148163
display id: range2148163
version: 1
direction: direction/forward
top level: lux pR-3
start: 53
end: 53
orientation: inline

LuxR/HSL
type: SequenceAnnotation
persistent identity: annotation2148166
display id: annotation2148166
version: 1
top level: lux pR-3
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K658006/annotation2148166/range2148166/1

https://synbiohub.org/public/igem/BBa_K658006/annotation2148166/range2148166/1
type: Range
persistent identity: range2148166
display id: range2148166
version: 1
direction: direction/forward
top level: lux pR-3
start: 1
end: 20
orientation: inline

BBa_I742163
type: ComponentDefinition
persistent identity: BBa I742163
display id: BBa_I742163
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: native ftsK rbs
creator: Chris French
created: 2007-10-24T11:00:00Z
modified: 2015-08-31T04:08:03Z
discontinued: false
dominant: false
experience: experience/None
group u list: _123_
m user id: 0
owner id: 837
owning group id: 163
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is the native ribosome binding site of ftsK, a DNA-translocating motor protein involved in cell division in Escherichia coli.
mutable notes: Virtual part to be added to coding sequence BBa_I742137.
mutable provenance: Escherichia coli JM109 (a K12 strain).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: rbs
sequence annotation: SacI
sequence: https://synbiohub.org/public/igem/BBa_I742163_sequence/1

rbs
type: SequenceAnnotation
persistent identity: annotation1954641
display id: annotation1954641
version: 1
top level: BBa_I742163
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_I742163/annotation1954641/range1954641/1

https://synbiohub.org/public/igem/BBa_I742163/annotation1954641/range1954641/1
type: Range
persistent identity: range1954641
display id: range1954641
version: 1
direction: direction/forward
top level: BBa_I742163
start: 15
end: 20
orientation: inline

SacI
type: SequenceAnnotation
persistent identity: annotation1954640
display id: annotation1954640
version: 1
top level: BBa_I742163
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I742163/annotation1954640/range1954640/1

https://synbiohub.org/public/igem/BBa_I742163/annotation1954640/range1954640/1
type: Range
persistent identity: range1954640
display id: range1954640
version: 1
direction: direction/forward
top level: BBa_I742163
start: 1
end: 3
orientation: inline

BBa_K165032
type: ComponentDefinition
persistent identity: BBa K165032
display id: BBa_K165032
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: mCYC promoter plus Gli1 binding sites
creator: Aaron Glieberman
created: 2008-10-28T12:00:00Z
modified: 2015-05-08T01:10:56Z
discontinued: false
dominant: false
group u list: _267_
m user id: 0
owner id: 2510
owning group id: 58
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This is a composite part containing the minimal promoter mCYC preceded by multiple consecutive binding sites for the Gli1 binding domain. This part contains the sequences for BBa_K165012 and BBa_K165016 but also has some additional DNA.
mutable notes: It was necessary to design primers for use in PCR to remove this part from its host plasmid and transfer it to a standard Biobrick vector.
mutable provenance: Composite part that was taken via PCR from a synthetic plasmid containing the sequence. The plasmid came from Lawrence Berkeley National Laboratory.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K165032_sequence/1

creD RBS
type: ComponentDefinition
persistent identity: BBa J64609
display id: BBa_J64609
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: creD RBS
creator: Ryan Ritterson
created: 2007-03-19T12:00:00Z
modified: 2015-05-08T01:08:17Z
discontinued: false
dominant: false
experience: experience/None
group u list: _98_
m user id: 0
owner id: 1422
owning group id: 98
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: The ribosomal binding site of the creD gene in the E Coli creD operon.
mutable notes: natural sequence, no design.
mutable provenance: E Coli Strain K12
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J64609_sequence/1

Mdaa
type: ComponentDefinition
persistent identity: BBa M36782
display id: BBa_M36782
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PoPS -> Melamine deaminase
creator: Ray Paul Laureano-Mercado & Mark Kirollos
created: 2013-06-09T11:00:00Z
modified: 2015-05-08T01:14:06Z
discontinued: false
dominant: false
experience: experience/None
group u list: _848_
m user id: 0
owner id: 17944
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This actuator's purpose is to code for the output of the enzyme melamine deaminase. This enzyme eliminates an amine group from melamine to produce ammonia and ammeline and is able to remove another amine group at a rate 10 times slower than the first reaction to bring the compound to ammelide. The enzyme does not react to a measurable extent with this last compound, so it metabolizes melamine by consecutive and predictable deaminations. Its design comprises three components, which include a bicistronic ribosome binding site, the MDA gene of interest which codes from melamine deaminase, and a transcription terminator. The part needs a PoPS signal as an input in order to produce the desired enzyme and was designed to work in E. coli.
mutable notes: The TriA gene???s codons were modified from the original sequence and optimized for E. coli to a Codon Adaptation Index (CAI) of .76 (Puigb??, P., Guzm??n, E., Romeu, A. & Garcia-Vallv??, S. OPTIMIZER: A web server for optimizing the codon usage of DNA sequences. Nucleic Acids Research. 2007;35:W126???31.). After that, some palindromic and mRNA destabilizing sequences were removed to increase the stability of our DNA during synthesis. The sequence shown maintains a CAI of .76 and the same amino acid sequence as the original TriA gene.
mutable provenance: The original gene coding for the protein of interest was isolated from a species of Pseudomonas (http://www.ncbi.nlm.nih.gov/nuccore/11890744). The sequences for the RBS and Terminator were sourced from BIOFAB (http://biofab.org/)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B1002
component: BBa_M36761
component: BBa_M36236
sequence annotation: BBa_B1002
sequence annotation: BBa_M36236
sequence annotation: BBa_M36761
sequence: https://synbiohub.org/public/igem/BBa_M36782_sequence/1

BBa_B1002
type: Component
persistent identity: component2329375
display id: component2329375
version: 1
top level: Mdaa
access: public
definition: BBa_B1002

BBa_M36761
type: Component
persistent identity: component2329370
display id: component2329370
version: 1
top level: Mdaa
access: public
definition: MDA

BBa_M36236
type: Component
persistent identity: component2329369
display id: component2329369
version: 1
top level: Mdaa
access: public
definition: https://synbiohub.org/public/igem/BBa_M36236/1

BBa_B1002
type: SequenceAnnotation
persistent identity: annotation2329375
display id: annotation2329375
version: 1
top level: Mdaa
location: https://synbiohub.org/public/igem/BBa_M36782/annotation2329375/range2329375/1
component: BBa_B1002

https://synbiohub.org/public/igem/BBa_M36782/annotation2329375/range2329375/1
type: Range
persistent identity: range2329375
display id: range2329375
version: 1
direction: direction/reverse
top level: Mdaa
start: 1511
end: 1544
orientation: inline

BBa_M36236
type: SequenceAnnotation
persistent identity: annotation2329369
display id: annotation2329369
version: 1
top level: Mdaa
location: https://synbiohub.org/public/igem/BBa_M36782/annotation2329369/range2329369/1
component: BBa_M36236

https://synbiohub.org/public/igem/BBa_M36782/annotation2329369/range2329369/1
type: Range
persistent identity: range2329369
display id: range2329369
version: 1
direction: direction/reverse
top level: Mdaa
start: 1
end: 85
orientation: inline

BBa_M36761
type: SequenceAnnotation
persistent identity: annotation2329370
display id: annotation2329370
version: 1
top level: Mdaa
location: https://synbiohub.org/public/igem/BBa_M36782/annotation2329370/range2329370/1
component: BBa_M36761

https://synbiohub.org/public/igem/BBa_M36782/annotation2329370/range2329370/1
type: Range
persistent identity: range2329370
display id: range2329370
version: 1
direction: direction/reverse
top level: Mdaa
start: 86
end: 1510
orientation: inline

BBa_K1486033
type: ComponentDefinition
persistent identity: BBa K1486033
display id: BBa_K1486033
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: R.reniformis luciferase + ADH1 terminator + CaUra3
creator: EPFL iGem team 2014
created: 2014-09-26T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: To edit
mutable notes: To edit
mutable provenance: To edit
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000324
role: partType/Tag
component: BBa_K1486037
component: BBa_K1486031
component: BBa_K1486025
component: BBa_K1486019
component: BBa_K1486020
sequence annotation: BBa_K1486020
sequence annotation: BBa_K1486031
sequence annotation: BBa_K1486037
sequence annotation: BBa_K1486025
sequence annotation: BBa_K1486019
sequence: https://synbiohub.org/public/igem/BBa_K1486033_sequence/1

BBa_K1486037
type: Component
persistent identity: component2418782
display id: component2418782
version: 1
top level: BBa_K1486033
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486037/1

BBa_K1486031
type: Component
persistent identity: component2418790
display id: component2418790
version: 1
top level: BBa_K1486033
access: public
definition: BBa_K1486031

BBa_K1486025
type: Component
persistent identity: component2418786
display id: component2418786
version: 1
top level: BBa_K1486033
access: public
definition: BBa_K1486025

BBa_K1486019
type: Component
persistent identity: component2418783
display id: component2418783
version: 1
top level: BBa_K1486033
access: public
definition: BBa_K1486019

BBa_K1486020
type: Component
persistent identity: component2418784
display id: component2418784
version: 1
top level: BBa_K1486033
access: public
definition: BBa_K1486020

BBa_K1486020
type: SequenceAnnotation
persistent identity: annotation2418784
display id: annotation2418784
version: 1
top level: BBa_K1486033
location: https://synbiohub.org/public/igem/BBa_K1486033/annotation2418784/range2418784/1
component: BBa_K1486020

https://synbiohub.org/public/igem/BBa_K1486033/annotation2418784/range2418784/1
type: Range
persistent identity: range2418784
display id: range2418784
version: 1
direction: direction/reverse
top level: BBa_K1486033
start: 370
end: 975
orientation: inline

BBa_K1486031
type: SequenceAnnotation
persistent identity: annotation2418790
display id: annotation2418790
version: 1
top level: BBa_K1486033
location: https://synbiohub.org/public/igem/BBa_K1486033/annotation2418790/range2418790/1
component: BBa_K1486031

https://synbiohub.org/public/igem/BBa_K1486033/annotation2418790/range2418790/1
type: Range
persistent identity: range2418790
display id: range2418790
version: 1
direction: direction/reverse
top level: BBa_K1486033
start: 1164
end: 2523
orientation: inline

BBa_K1486037
type: SequenceAnnotation
persistent identity: annotation2418782
display id: annotation2418782
version: 1
top level: BBa_K1486033
location: https://synbiohub.org/public/igem/BBa_K1486033/annotation2418782/range2418782/1
component: BBa_K1486037

https://synbiohub.org/public/igem/BBa_K1486033/annotation2418782/range2418782/1
type: Range
persistent identity: range2418782
display id: range2418782
version: 1
direction: direction/reverse
top level: BBa_K1486033
start: 1
end: 39
orientation: inline

BBa_K1486025
type: SequenceAnnotation
persistent identity: annotation2418786
display id: annotation2418786
version: 1
top level: BBa_K1486033
location: https://synbiohub.org/public/igem/BBa_K1486033/annotation2418786/range2418786/1
component: BBa_K1486025

https://synbiohub.org/public/igem/BBa_K1486033/annotation2418786/range2418786/1
type: Range
persistent identity: range2418786
display id: range2418786
version: 1
direction: direction/reverse
top level: BBa_K1486033
start: 976
end: 1163
orientation: inline

BBa_K1486019
type: SequenceAnnotation
persistent identity: annotation2418783
display id: annotation2418783
version: 1
top level: BBa_K1486033
location: https://synbiohub.org/public/igem/BBa_K1486033/annotation2418783/range2418783/1
component: BBa_K1486019

https://synbiohub.org/public/igem/BBa_K1486033/annotation2418783/range2418783/1
type: Range
persistent identity: range2418783
display id: range2418783
version: 1
direction: direction/reverse
top level: BBa_K1486033
start: 40
end: 369
orientation: inline

BBa_K1486034
type: ComponentDefinition
persistent identity: BBa K1486034
display id: BBa_K1486034
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Yeast optimized superfolder GFP C-terminus (215-238)
creator: EPFL iGem team 2014
created: 2014-09-26T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: To edit
mutable notes: To edit
mutable provenance: To edit
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000324
role: partType/Tag
sequence annotation: Yeast optimized sfGFP C-terminus
sequence: https://synbiohub.org/public/igem/BBa_K1486034_sequence/1

Yeast optimized sfGFP C-terminus
type: SequenceAnnotation
persistent identity: annotation2417784
display id: annotation2417784
version: 1
top level: BBa_K1486034
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1486034/annotation2417784/range2417784/1

https://synbiohub.org/public/igem/BBa_K1486034/annotation2417784/range2417784/1
type: Range
persistent identity: range2417784
display id: range2417784
version: 1
direction: direction/forward
top level: BBa_K1486034
start: 1
end: 75
orientation: inline

U049O11+D0
type: ComponentDefinition
persistent identity: BBa I732440
display id: BBa_I732440
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Family Member (U049O11+D038O22)
creator: Zhan Jian
created: 2007-10-13T11:00:00Z
modified: 2015-08-31T04:07:58Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732440_sequence/1

BBa_K137087
type: ComponentDefinition
persistent identity: BBa K137087
display id: BBa_K137087
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: optimized (TA) repeat constitutive promoter with 17 bp between -10 and -35 elements
creator: Allen Lin
created: 2008-08-20T11:00:00Z
modified: 2015-05-08T01:10:10Z
discontinued: false
dominant: false
experience: experience/None
group u list: _187_
m user id: 0
owner id: 3112
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: optimized (TA) repeat constitutive promoter with 17 bp between -10 and -35 elements
mutable notes: None
mutable provenance: PCR
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K137087_sequence/1

BBa_K086031
type: ComponentDefinition
persistent identity: BBa K086031
display id: BBa_K086031
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: modified Lutz-Bujard LacO promoter,with alternative sigma factor σ38
creator: Gairik Sachdeva
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:08:37Z
discontinued: false
dominant: false
group u list: _197_
m user id: 0
owner id: 2578
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: modified Lutz-Bujard LacO promoter,with alternative sigma factor σ38
mutable notes: Cleaned
mutable provenance: Lac O and sigma 38 binding sites conserved sequences.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: P38_2
sequence: https://synbiohub.org/public/igem/BBa_K086031_sequence/1

P38_2
type: SequenceAnnotation
persistent identity: annotation1995765
display id: annotation1995765
version: 1
top level: BBa_K086031
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K086031/annotation1995765/range1995765/1

https://synbiohub.org/public/igem/BBa_K086031/annotation1995765/range1995765/1
type: Range
persistent identity: range1995765
display id: range1995765
version: 1
direction: direction/forward
top level: BBa_K086031
start: 1
end: 55
orientation: inline

BBa_K1486044
type: ComponentDefinition
persistent identity: BBa K1486044
display id: BBa_K1486044
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: IFP[1] Mutated
creator: EPFL iGem team 2014
created: 2014-10-04T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This part is the twin of the non-compatible biobrick BBa_K1486008 rendered compatible by mutating the two illegal restriction sites. However, all experiments were run on the aforementioned biobrick.
mutable notes: Site-directed mutagenesis was used to remove the restriction sites in the sequence.
mutable provenance: http://www.addgene.org/browse/article/8177/ which was then mutated by us.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: IFP1_Mut
sequence: https://synbiohub.org/public/igem/BBa_K1486044_sequence/1

IFP1_Mut
type: SequenceAnnotation
persistent identity: annotation2420792
display id: annotation2420792
version: 1
top level: BBa_K1486044
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1486044/annotation2420792/range2420792/1

https://synbiohub.org/public/igem/BBa_K1486044/annotation2420792/range2420792/1
type: Range
persistent identity: range2420792
display id: range2420792
version: 1
direction: direction/forward
top level: BBa_K1486044
start: 1
end: 402
orientation: inline

BBa_K137085
type: ComponentDefinition
persistent identity: BBa K137085
display id: BBa_K137085
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: optimized (TA) repeat constitutive promoter with 13 bp between -10 and -35 elements
creator: Allen Lin
created: 2008-08-20T11:00:00Z
modified: 2015-05-08T01:10:10Z
discontinued: false
dominant: false
experience: experience/None
group u list: _187_
m user id: 0
owner id: 3112
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: optimized (TA) repeat constitutive promoter with 13 bp between -10 and -35 elements
mutable notes: None
mutable provenance: PCR
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K137085_sequence/1

BBa_B0029
type: ComponentDefinition
persistent identity: BBa B0029
display id: BBa_B0029
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: 1 bp deletion from B0031
creator: Jason Kelly
created: 2008-02-07T12:00:00Z
modified: 2015-08-31T04:07:20Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_
m user id: 0
owner id: 2
owning group id: 84
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: .
mutable notes: .
mutable provenance: .
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_B0029_sequence/1

BBa_J61103
type: ComponentDefinition
persistent identity: BBa J61103
display id: BBa_J61103
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Ribosome Binding Site Family Member
creator: John Anderson
created: 2007-04-23T11:00:00Z
modified: 2015-08-31T02:03:00Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: false
mutable description: {{JCA_Arkin_RBSFamily}}
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J61103_sequence/1

BBa_J34810
type: ComponentDefinition
persistent identity: BBa J34810
display id: BBa_J34810
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: ribosome binding site
creator: Alexandra Choutko
created: 2006-10-25T11:00:00Z
modified: 2015-08-31T04:08:47Z
discontinued: false
dominant: false
experience: experience/None
group u list: _62_
m user id: 0
owner id: 1085
owning group id: 62
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: a
mutable notes: a
mutable provenance: a
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J34810_sequence/1

BBa_K1585100
type: ComponentDefinition
persistent identity: BBa K1585100
display id: BBa_K1585100
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Anderson Promoter with lacI binding site
creator: Tobias Schwanemann
created: 2015-08-23T11:00:00Z
modified: 2015-08-26T11:28:17Z
discontinued: false
dominant: false
experience: experience/Fails
group u list: _2002_
m user id: 24474
owner id: 24474
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Promoters of the Anderson Promoter Collection with lacI binding site derived from [http://parts.igem.org/Part:BBa_R0010 R_0010] to make them [http://openwetware.org/wiki/IPTG IPTG] iducible.
mutable notes: The "lacI binding site" of Part [http://parts.igem.org/Part:BBa_R0010 R_0010] differs from lacO from Part [http://parts.igem.org/Part:BBa_J33207 BBa_J33207] although they should be same. We choosed part BBa_R0010 because we assumed older parts would be reliable.
mutable provenance: Assambly of [http://parts.igem.org/Part:BBa_J23100 J23100] and lacI-binding-site of [http://parts.igem.org/Part:BBa_R0010 R_0010]
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
component: BBa_K1585999
component: BBa_J23100
sequence annotation: BBa_K1585999
sequence annotation: BBa_J23100
sequence: https://synbiohub.org/public/igem/BBa_K1585100_sequence/1

BBa_K1585999
type: Component
persistent identity: component2436913
display id: component2436913
version: 1
top level: BBa_K1585100
access: public
definition: https://synbiohub.org/public/igem/BBa_K1585999/1

BBa_J23100
type: Component
persistent identity: component2436912
display id: component2436912
version: 1
top level: BBa_K1585100
access: public
definition: BBa_J23100

BBa_K1585999
type: SequenceAnnotation
persistent identity: annotation2436913
display id: annotation2436913
version: 1
top level: BBa_K1585100
location: https://synbiohub.org/public/igem/BBa_K1585100/annotation2436913/range2436913/1
component: BBa_K1585999

https://synbiohub.org/public/igem/BBa_K1585100/annotation2436913/range2436913/1
type: Range
persistent identity: range2436913
display id: range2436913
version: 1
direction: direction/reverse
top level: BBa_K1585100
start: 44
end: 78
orientation: inline

BBa_J23100
type: SequenceAnnotation
persistent identity: annotation2436912
display id: annotation2436912
version: 1
top level: BBa_K1585100
location: https://synbiohub.org/public/igem/BBa_K1585100/annotation2436912/range2436912/1
component: BBa_J23100

https://synbiohub.org/public/igem/BBa_K1585100/annotation2436912/range2436912/1
type: Range
persistent identity: range2436912
display id: range2436912
version: 1
direction: direction/reverse
top level: BBa_K1585100
start: 1
end: 35
orientation: inline

U097NUL+D0
type: ComponentDefinition
persistent identity: BBa I732412
display id: BBa_I732412
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Family Member (U097NUL+D062O22)
creator: Zhan Jian
created: 2007-09-22T11:00:00Z
modified: 2015-08-31T04:07:58Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1572
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: To be later edited if time permits.
mutable notes: To be later edited if time permits.
mutable provenance: To be later edited if time permits.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: consensus elements
sequence annotation: +1
sequence annotation: O2
sequence: https://synbiohub.org/public/igem/BBa_I732412_sequence/1

consensus elements
type: SequenceAnnotation
persistent identity: annotation1944564
display id: annotation1944564
version: 1
top level: U097NUL+D0
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I732412/annotation1944564/range1944564/1

https://synbiohub.org/public/igem/BBa_I732412/annotation1944564/range1944564/1
type: Range
persistent identity: range1944564
display id: range1944564
version: 1
direction: direction/forward
top level: U097NUL+D0
start: 61
end: 137
orientation: inline

+1
type: SequenceAnnotation
persistent identity: annotation1944566
display id: annotation1944566
version: 1
top level: U097NUL+D0
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_I732412/annotation1944566/range1944566/1

https://synbiohub.org/public/igem/BBa_I732412/annotation1944566/range1944566/1
type: Range
persistent identity: range1944566
display id: range1944566
version: 1
direction: direction/forward
top level: U097NUL+D0
start: 137
end: 137
orientation: inline

O2
type: SequenceAnnotation
persistent identity: annotation1944565
display id: annotation1944565
version: 1
top level: U097NUL+D0
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_I732412/annotation1944565/range1944565/1

https://synbiohub.org/public/igem/BBa_I732412/annotation1944565/range1944565/1
type: Range
persistent identity: range1944565
display id: range1944565
version: 1
direction: direction/forward
top level: U097NUL+D0
start: 158
end: 177
orientation: inline

BBa_K113009
type: ComponentDefinition
persistent identity: BBa K113009
display id: BBa_K113009
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pBad/araC
creator: Zheng Wei, Wang Jinyu
created: 2008-10-25T11:00:00Z
modified: 2015-05-08T01:09:20Z
discontinued: false
dominant: false
experience: experience/None
group u list: _254_
m user id: 0
owner id: 2711
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: It is the same as BBa_I0050. But the part seems not working so we make a new one, which the template is from iHKU team.
mutable notes: later
mutable provenance: iHKU team
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K113009_sequence/1

pBAD
type: ComponentDefinition
persistent identity: BBa K206000
display id: BBa_K206000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pBAD strong
creator: Amelia Hardjasa
created: 2009-10-13T11:00:00Z
modified: 2015-05-08T01:11:23Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _307_
m user id: 0
owner id: 4172
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: pBAD is an E.coli promoter that is induced by L-arabinose. K206000 is a mutagenized pBAD promoter that is responsive to lower concentrations of arabinose than wild type ( I13453 ) and, additionally, exhibits a higher maximum of protein expression as measured by coupling to a fluorescent reporter.
mutable notes: There were no special design considerations.
mutable provenance: The sequence was obtained by applying all mutations that upregulated AraC binding and subsequent promoter activity listed in reference [1].
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: promoter
sequence annotation: AraI1
sequence annotation: AraI2
sequence: https://synbiohub.org/public/igem/BBa_K206000_sequence/1

promoter
type: SequenceAnnotation
persistent identity: annotation2049252
display id: annotation2049252
version: 1
top level: pBAD
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K206000/annotation2049252/range2049252/1

https://synbiohub.org/public/igem/BBa_K206000/annotation2049252/range2049252/1
type: Range
persistent identity: range2049252
display id: range2049252
version: 1
direction: direction/forward
top level: pBAD
start: 1
end: 131
orientation: inline

AraI1
type: SequenceAnnotation
persistent identity: annotation2049253
display id: annotation2049253
version: 1
top level: pBAD
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K206000/annotation2049253/range2049253/1

https://synbiohub.org/public/igem/BBa_K206000/annotation2049253/range2049253/1
type: Range
persistent identity: range2049253
display id: range2049253
version: 1
direction: direction/forward
top level: pBAD
start: 40
end: 57
orientation: inline

AraI2
type: SequenceAnnotation
persistent identity: annotation2049254
display id: annotation2049254
version: 1
top level: pBAD
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K206000/annotation2049254/range2049254/1

https://synbiohub.org/public/igem/BBa_K206000/annotation2049254/range2049254/1
type: Range
persistent identity: range2049254
display id: range2049254
version: 1
direction: direction/forward
top level: pBAD
start: 61
end: 78
orientation: inline

BBa_I766214
type: ComponentDefinition
persistent identity: BBa I766214
display id: BBa_I766214
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pGal1
creator: Nili Sommovilla
created: 2007-10-21T11:00:00Z
modified: 2015-08-31T04:08:09Z
discontinued: false
dominant: false
group u list: _155_
m user id: 0
owner id: 1931
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: pGAl1 promoter region
mutable notes: none
mutable provenance: yeast
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I766214_sequence/1

BBa_K598000
type: ComponentDefinition
persistent identity: BBa K598000
display id: BBa_K598000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TPP Down-regulated Hammerhead Ribozyme 2.5 with Native RBS+E0040+B0015
creator: Yangyang ZHAO, Yan GONG, Zhenrun ZHANG, Qingyang XIAO
created: 2011-09-17T11:00:00Z
modified: 2015-05-08T01:12:50Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _770_
m user id: 0
owner id: 4411
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: This part is TPP up-regulated hammerhead ribozyme, consisting of 131 nucleotides.
mutable notes: This part contains a native ribosomal binding site
mutable provenance: This part was an engineered RNA controller composed of a TPP aptamer from B.subtilis and a hammerhead motif.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: polya
sequence annotation: native RBS
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence annotation: TPP Ribozyme 2.5
sequence annotation: T7 TE
sequence annotation: stop
sequence annotation: BBa_E0040
sequence annotation: eGFP
sequence annotation: BBa_B0012
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_K598000_sequence/1

polya
type: SequenceAnnotation
persistent identity: annotation2152870
display id: annotation2152870
version: 1
top level: BBa_K598000
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_K598000/annotation2152870/range2152870/1

https://synbiohub.org/public/igem/BBa_K598000/annotation2152870/range2152870/1
type: Range
persistent identity: range2152870
display id: range2152870
version: 1
direction: direction/forward
top level: BBa_K598000
start: 994
end: 1007
orientation: inline

native RBS
type: SequenceAnnotation
persistent identity: annotation2131876
display id: annotation2131876
version: 1
top level: BBa_K598000
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K598000/annotation2131876/range2131876/1

https://synbiohub.org/public/igem/BBa_K598000/annotation2131876/range2131876/1
type: Range
persistent identity: range2131876
display id: range2131876
version: 1
direction: direction/forward
top level: BBa_K598000
start: 138
end: 145
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2152836
display id: annotation2152836
version: 1
top level: BBa_K598000
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K598000/annotation2152836/range2152836/1

https://synbiohub.org/public/igem/BBa_K598000/annotation2152836/range2152836/1
type: Range
persistent identity: range2152836
display id: range2152836
version: 1
direction: direction/forward
top level: BBa_K598000
start: 879
end: 958
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation2152857
display id: annotation2152857
version: 1
top level: BBa_K598000
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K598000/annotation2152857/range2152857/1

https://synbiohub.org/public/igem/BBa_K598000/annotation2152857/range2152857/1
type: Range
persistent identity: range2152857
display id: range2152857
version: 1
direction: direction/forward
top level: BBa_K598000
start: 890
end: 933
orientation: inline

TPP Ribozyme 2.5
type: SequenceAnnotation
persistent identity: annotation2152875
display id: annotation2152875
version: 1
top level: BBa_K598000
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K598000/annotation2152875/range2152875/1

https://synbiohub.org/public/igem/BBa_K598000/annotation2152875/range2152875/1
type: Range
persistent identity: range2152875
display id: range2152875
version: 1
direction: direction/forward
top level: BBa_K598000
start: 1
end: 150
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation2152868
display id: annotation2152868
version: 1
top level: BBa_K598000
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K598000/annotation2152868/range2152868/1

https://synbiohub.org/public/igem/BBa_K598000/annotation2152868/range2152868/1
type: Range
persistent identity: range2152868
display id: range2152868
version: 1
direction: direction/forward
top level: BBa_K598000
start: 974
end: 993
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2152872
display id: annotation2152872
version: 1
top level: BBa_K598000
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K598000/annotation2152872/range2152872/1

https://synbiohub.org/public/igem/BBa_K598000/annotation2152872/range2152872/1
type: Range
persistent identity: range2152872
display id: range2152872
version: 1
direction: direction/forward
top level: BBa_K598000
start: 1000
end: 1000
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation2152880
display id: annotation2152880
version: 1
top level: BBa_K598000
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K598000/annotation2152880/range2152880/1

https://synbiohub.org/public/igem/BBa_K598000/annotation2152880/range2152880/1
type: Range
persistent identity: range2152880
display id: range2152880
version: 1
direction: direction/forward
top level: BBa_K598000
start: 151
end: 870
orientation: inline

eGFP
type: SequenceAnnotation
persistent identity: annotation2152835
display id: annotation2152835
version: 1
top level: BBa_K598000
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K598000/annotation2152835/range2152835/1

https://synbiohub.org/public/igem/BBa_K598000/annotation2152835/range2152835/1
type: Range
persistent identity: range2152835
display id: range2152835
version: 1
direction: direction/forward
top level: BBa_K598000
start: 151
end: 870
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2152867
display id: annotation2152867
version: 1
top level: BBa_K598000
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K598000/annotation2152867/range2152867/1

https://synbiohub.org/public/igem/BBa_K598000/annotation2152867/range2152867/1
type: Range
persistent identity: range2152867
display id: range2152867
version: 1
direction: direction/forward
top level: BBa_K598000
start: 967
end: 1007
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2153033
display id: annotation2153033
version: 1
top level: BBa_K598000
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K598000/annotation2153033/range2153033/1

https://synbiohub.org/public/igem/BBa_K598000/annotation2153033/range2153033/1
type: Range
persistent identity: range2153033
display id: range2153033
version: 1
direction: direction/forward
top level: BBa_K598000
start: 879
end: 1007
orientation: inline

BBa_K1315004
type: ComponentDefinition
persistent identity: BBa K1315004
display id: BBa_K1315004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: ydeH (dgcZ)
creator: Dimitrios Michailidis
created: 2014-09-24T11:00:00Z
modified: 2015-05-08T01:09:49Z
discontinued: true
dominant: false
experience: experience/None
group u list: _1690_
m user id: 0
owner id: 21812
owning group id: 9
sample status: Discontinued
status: status/Deleted
bookmark: false
mutable description: Diguanylate cyclases produce cyclic diGMP (c-di-GMP), a secondary messenger. Diguanylate Cyclase (DgcZ), also called YdeH, is present in Escherichia coli and the stimulus that controls it is Zinc.
mutable notes: The sequence did not have any restriction sites to remove so it was immediately PCRed out of the genome and had the registry prefix and suffix added to it. It was then ligated into the pSB1C3 vector. YdeH however, is toxic to E. coli cells.
mutable provenance: The source of this part is Escherichia coli MC40100 genomic DNA which was provided by the Tracy Palmer lab in the Molecular Microbiology department of the University of Dundee, UK.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: YdeH
sequence: https://synbiohub.org/public/igem/BBa_K1315004_sequence/1

YdeH
type: SequenceAnnotation
persistent identity: annotation2386526
display id: annotation2386526
version: 1
top level: BBa_K1315004
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1315004/annotation2386526/range2386526/1

https://synbiohub.org/public/igem/BBa_K1315004/annotation2386526/range2386526/1
type: Range
persistent identity: range2386526
display id: range2386526
version: 1
direction: direction/forward
top level: BBa_K1315004
start: 1
end: 894
orientation: inline

PrFe
type: ComponentDefinition
persistent identity: BBa J3902
display id: BBa_J3902
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PrFe (PI + PII rus operon)
creator: Latin American iGEM team
created: 2006-10-28T11:00:00Z
modified: 2015-08-31T04:08:48Z
discontinued: false
dominant: false
experience: experience/None
group u list: _67_
m user id: 0
owner id: 1000
owning group id: 67
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Promoter region from Acidithiobacillus ferrooxidans rus operon
mutable notes: None
mutable provenance: Sequence found at NCBI (AJ006456)
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: promoter
sequence: https://synbiohub.org/public/igem/BBa_J3902_sequence/1

promoter
type: SequenceAnnotation
persistent identity: annotation1908375
display id: annotation1908375
version: 1
top level: PrFe
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_J3902/annotation1908375/range1908375/1

https://synbiohub.org/public/igem/BBa_J3902/annotation1908375/range1908375/1
type: Range
persistent identity: range1908375
display id: range1908375
version: 1
direction: direction/forward
top level: PrFe
start: 7
end: 266
orientation: inline

MioC
type: ComponentDefinition
persistent identity: BBa K101017
display id: BBa_K101017
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: MioC Promoter (DNAa-Repressed Promoter)
creator: Karl Petersen
created: 2008-10-27T12:00:00Z
modified: 2015-05-08T01:08:42Z
discontinued: false
dominant: false
experience: experience/None
group u list: _205_
m user id: 0
owner id: 2728
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: The MioC promoter is a cell-cycle dependent promoter that is repressed before initiation of replication and depressed shortly after. The regulation of the MioC gene is overseen by several DNAa proteins, which bind along the promoter. This particular MioC promoter was isolated from K-12 (JM109). This promoter can be used in series with a reporter to signal only during a given part of the cell cycle.
mutable notes: Site directed mutagenesis was used to create a Spe1 restriction site.
mutable provenance: The source of this part is from the genomic dna of E.coli (JM109).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: DNAa Binding Box
sequence annotation: MioC Promoter
sequence: https://synbiohub.org/public/igem/BBa_K101017_sequence/1

DNAa Binding Box
type: SequenceAnnotation
persistent identity: annotation1992605
display id: annotation1992605
version: 1
top level: MioC
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K101017/annotation1992605/range1992605/1

https://synbiohub.org/public/igem/BBa_K101017/annotation1992605/range1992605/1
type: Range
persistent identity: range1992605
display id: range1992605
version: 1
direction: direction/forward
top level: MioC
start: 283
end: 292
orientation: inline

DNAa Binding Box
type: SequenceAnnotation
persistent identity: annotation1992601
display id: annotation1992601
version: 1
top level: MioC
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K101017/annotation1992601/range1992601/1

https://synbiohub.org/public/igem/BBa_K101017/annotation1992601/range1992601/1
type: Range
persistent identity: range1992601
display id: range1992601
version: 1
direction: direction/forward
top level: MioC
start: 295
end: 303
orientation: inline

DNAa Binding Box
type: SequenceAnnotation
persistent identity: annotation1992609
display id: annotation1992609
version: 1
top level: MioC
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K101017/annotation1992609/range1992609/1

https://synbiohub.org/public/igem/BBa_K101017/annotation1992609/range1992609/1
type: Range
persistent identity: range1992609
display id: range1992609
version: 1
direction: direction/forward
top level: MioC
start: 159
end: 167
orientation: inline

MioC Promoter
type: SequenceAnnotation
persistent identity: annotation1992615
display id: annotation1992615
version: 1
top level: MioC
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K101017/annotation1992615/range1992615/1

https://synbiohub.org/public/igem/BBa_K101017/annotation1992615/range1992615/1
type: Range
persistent identity: range1992615
display id: range1992615
version: 1
direction: direction/forward
top level: MioC
start: 131
end: 319
orientation: inline

DNAa Binding Box
type: SequenceAnnotation
persistent identity: annotation1992607
display id: annotation1992607
version: 1
top level: MioC
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K101017/annotation1992607/range1992607/1

https://synbiohub.org/public/igem/BBa_K101017/annotation1992607/range1992607/1
type: Range
persistent identity: range1992607
display id: range1992607
version: 1
direction: direction/forward
top level: MioC
start: 273
end: 279
orientation: inline

BBa_K1072022
type: ComponentDefinition
persistent identity: BBa K1072022
display id: BBa_K1072022
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Lux pI+RBS+LuxR+2TM+Lux pR+RBS+LuxI+2TM+Lux pR+RBS+GFP+2TM+Lux pR+RBS+Ndh+2TM
creator: Yichong wang, Ruichen and Junjie zhang
created: 2013-09-07T11:00:00Z
modified: 2015-05-08T01:09:01Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1381_
m user id: 0
owner id: 16002
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: -
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0012
component: BBa_B0010
component: BBa_C0061
component: BBa_R0062
component: BBa_B0034
component: BBa_R0063
component: BBa_C0062
component: BBa_K1072001
component: BBa_E0040
sequence annotation: BBa_B0034
sequence annotation: BBa_B0012
sequence annotation: BBa_R0062
sequence annotation: BBa_K1072001
sequence annotation: BBa_C0061
sequence annotation: BBa_R0063
sequence annotation: BBa_B0010
sequence annotation: BBa_E0040
sequence annotation: BBa_C0062
sequence: https://synbiohub.org/public/igem/BBa_K1072022_sequence/1

BBa_B0012
type: Component
persistent identity: component2336216
display id: component2336216
version: 1
top level: BBa_K1072022
access: public
definition: BBa_B0012

BBa_B0010
type: Component
persistent identity: component2336214
display id: component2336214
version: 1
top level: BBa_K1072022
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_C0061
type: Component
persistent identity: component2336211
display id: component2336211
version: 1
top level: BBa_K1072022
access: public
definition: https://synbiohub.org/public/igem/BBa_C0061/1

BBa_B0012
type: Component
persistent identity: component2336231
display id: component2336231
version: 1
top level: BBa_K1072022
access: public
definition: BBa_B0012

BBa_B0012
type: Component
persistent identity: component2336199
display id: component2336199
version: 1
top level: BBa_K1072022
access: public
definition: BBa_B0012

BBa_B0010
type: Component
persistent identity: component2336244
display id: component2336244
version: 1
top level: BBa_K1072022
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_R0062
type: Component
persistent identity: component2336221
display id: component2336221
version: 1
top level: BBa_K1072022
access: public
definition: lux pR

BBa_B0034
type: Component
persistent identity: component2336241
display id: component2336241
version: 1
top level: BBa_K1072022
access: public
definition: BBa_B0034

BBa_R0063
type: Component
persistent identity: component2336184
display id: component2336184
version: 1
top level: BBa_K1072022
access: public
definition: lux pL

BBa_C0062
type: Component
persistent identity: component2336193
display id: component2336193
version: 1
top level: BBa_K1072022
access: public
definition: https://synbiohub.org/public/igem/BBa_C0062/1

BBa_R0062
type: Component
persistent identity: component2336204
display id: component2336204
version: 1
top level: BBa_K1072022
access: public
definition: lux pR

BBa_B0034
type: Component
persistent identity: component2336209
display id: component2336209
version: 1
top level: BBa_K1072022
access: public
definition: BBa_B0034

BBa_B0010
type: Component
persistent identity: component2336229
display id: component2336229
version: 1
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BBa_B0034
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BBa_C0061
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BBa_R0063
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BBa_B0012
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BBa_E0040
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BBa_B0010
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BBa_B0010
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BBa_B0034
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persistent identity: range2336190
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BBa_B0012
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BBa_C0062
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BBa_B0010
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BBa_B0034
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display id: annotation2336226
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BBa_K256018
type: ComponentDefinition
persistent identity: BBa K256018
display id: BBa_K256018
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: J23119:IFP
creator: iGEM09_NTU-Singapore
created: 2009-10-16T11:00:00Z
modified: 2015-05-08T01:11:41Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _359_
m user id: 0
owner id: 4864
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: IFP Generator
mutable notes: --
mutable provenance: --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_J23119
component: BBa_B0012
component: BBa_K256008
component: BBa_B0034
component: BBa_B0010
sequence annotation: BBa_B0034
sequence annotation: BBa_B0012
sequence annotation: BBa_J23119
sequence annotation: BBa_K256008
sequence annotation: BBa_B0010
sequence: https://synbiohub.org/public/igem/BBa_K256018_sequence/1

BBa_J23119
type: Component
persistent identity: component2048508
display id: component2048508
version: 1
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access: public
definition: BBa_J23119

BBa_B0012
type: Component
persistent identity: component2048514
display id: component2048514
version: 1
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access: public
definition: BBa_B0012

BBa_K256008
type: Component
persistent identity: component2048511
display id: component2048511
version: 1
top level: BBa_K256018
access: public
definition: https://synbiohub.org/public/igem/BBa_K256008/1

BBa_B0034
type: Component
persistent identity: component2048510
display id: component2048510
version: 1
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access: public
definition: BBa_B0034

BBa_B0010
type: Component
persistent identity: component2048512
display id: component2048512
version: 1
top level: BBa_K256018
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2048510
display id: annotation2048510
version: 1
top level: BBa_K256018
location: https://synbiohub.org/public/igem/BBa_K256018/annotation2048510/range2048510/1
component: BBa_B0034

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persistent identity: range2048510
display id: range2048510
version: 1
direction: direction/reverse
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start: 44
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orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2048514
display id: annotation2048514
version: 1
top level: BBa_K256018
location: https://synbiohub.org/public/igem/BBa_K256018/annotation2048514/range2048514/1
component: BBa_B0012

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type: Range
persistent identity: range2048514
display id: range2048514
version: 1
direction: direction/forward
top level: BBa_K256018
start: 1127
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orientation: inline

BBa_J23119
type: SequenceAnnotation
persistent identity: annotation2048508
display id: annotation2048508
version: 1
top level: BBa_K256018
location: https://synbiohub.org/public/igem/BBa_K256018/annotation2048508/range2048508/1
component: BBa_J23119

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persistent identity: range2048508
display id: range2048508
version: 1
direction: direction/reverse
top level: BBa_K256018
start: 1
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orientation: inline

BBa_K256008
type: SequenceAnnotation
persistent identity: annotation2048511
display id: annotation2048511
version: 1
top level: BBa_K256018
location: https://synbiohub.org/public/igem/BBa_K256018/annotation2048511/range2048511/1
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type: Range
persistent identity: range2048511
display id: range2048511
version: 1
direction: direction/reverse
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start: 62
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orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2048512
display id: annotation2048512
version: 1
top level: BBa_K256018
location: https://synbiohub.org/public/igem/BBa_K256018/annotation2048512/range2048512/1
component: BBa_B0010

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type: Range
persistent identity: range2048512
display id: range2048512
version: 1
direction: direction/forward
top level: BBa_K256018
start: 1039
end: 1118
orientation: inline

BBa_S05324
type: ComponentDefinition
persistent identity: BBa S05324
display id: BBa_S05324
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: J23101:K592024
creator: Trevor Y.H. Ho
created: 2016-04-11T11:00:00Z
modified: 2016-04-21T12:54:00Z
discontinued: true
dominant: false
experience: experience/None
group u list: _9_
m user id: 8758
owner id: 8758
owning group id: 9
status: status/Deleted
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_J23101
component: BBa_K592024
sequence annotation: BBa_J23101
sequence annotation: BBa_K592024
sequence: https://synbiohub.org/public/igem/BBa_S05324_sequence/1

BBa_J23101
type: Component
persistent identity: component2478621
display id: component2478621
version: 1
top level: BBa_S05324
access: public
definition: https://synbiohub.org/public/igem/BBa_J23101/1

BBa_K592024
type: Component
persistent identity: component2478626
display id: component2478626
version: 1
top level: BBa_S05324
access: public
definition: https://synbiohub.org/public/igem/BBa_K592024/1

BBa_J23101
type: SequenceAnnotation
persistent identity: annotation2478621
display id: annotation2478621
version: 1
top level: BBa_S05324
location: https://synbiohub.org/public/igem/BBa_S05324/annotation2478621/range2478621/1
component: BBa_J23101

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type: Range
persistent identity: range2478621
display id: range2478621
version: 1
direction: direction/reverse
top level: BBa_S05324
start: 1
end: 35
orientation: inline

BBa_K592024
type: SequenceAnnotation
persistent identity: annotation2478626
display id: annotation2478626
version: 1
top level: BBa_S05324
location: https://synbiohub.org/public/igem/BBa_S05324/annotation2478626/range2478626/1
component: BBa_K592024

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type: Range
persistent identity: range2478626
display id: range2478626
version: 1
direction: direction/reverse
top level: BBa_S05324
start: 44
end: 766
orientation: inline

U097O11+D0
type: ComponentDefinition
persistent identity: BBa I732417
display id: BBa_I732417
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Family Member (U097O11+D050O22)
creator: Zhan Jian
created: 2007-10-13T11:00:00Z
modified: 2015-08-31T04:07:58Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732417_sequence/1

p(osmY)
type: ComponentDefinition
persistent identity: BBa J45992
display id: BBa_J45992
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Full-length stationary phase osmY promoter
creator: Stephen Payne
created: 2006-07-10T11:00:00Z
modified: 2015-08-31T04:08:50Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _84_
m user id: 0
owner id: 642
owning group id: 84
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: osmY's promoter is active in stationary phase and under high osmotic pressure conditions.
mutable notes: Truncated Forward Primer: 5'- GTT TCT TCG AAT TCG CGG CCG CTT CTA GGCT TAT GTT TTC GCT GAT ATC - 3' Total Length: 49 bp Annealing Length: 21 bp GC Content: 38.1% Melting Temperature: 49.5 degrees C hairpin deltaG: -2.66 kcal/mol self dimer deltaG: -99.97 kcal/mol Full Forward Primer: 5'- GTT TCT TCG AAT TCG CGG CCG CTT CTA GCTG GCA CAG GAA CGT TAT C - 3' Total Length: 47 bp Annealing Length: 19 bp GC Content: 52.6% Melting Temperature: 53.4 degrees C hairpin deltaG: -3.99 kcal/mol self dimer deltaG: -98.3 kcal/mol Reverse Primer: 5'-GTT TCT TCC TGC AGC GGC CGC TAC TAG TAT TGT TAA ATA TAG ATC ACA ATT TTG- 3' Total Length: 54 bp Annealing Length: 25 bp GC Content: 20.0% Melting Temperature: 46.4 degrees C hairpin deltaG: -2.86 kcal/mol self dimer deltaG: -99.44 kcal/mol heterodimer deltaG with Forward Primer: -99.44 kcal/mol heterodimer deltaG with Conserved Forward Primer: -99.97 kcal/mol
mutable provenance: This part was PCRed out of E. coli's genome.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -35
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_J45992_sequence/1

-35
type: SequenceAnnotation
persistent identity: annotation1883646
display id: annotation1883646
version: 1
top level: p(osmY)
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_J45992/annotation1883646/range1883646/1

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type: Range
persistent identity: range1883646
display id: range1883646
version: 1
direction: direction/forward
top level: p(osmY)
start: 164
end: 169
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1883645
display id: annotation1883645
version: 1
top level: p(osmY)
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_J45992/annotation1883645/range1883645/1

https://synbiohub.org/public/igem/BBa_J45992/annotation1883645/range1883645/1
type: Range
persistent identity: range1883645
display id: range1883645
version: 1
direction: direction/forward
top level: p(osmY)
start: 188
end: 193
orientation: inline

mRFP1
type: ComponentDefinition
persistent identity: BBa K678030
display id: BBa_K678030
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: mRFP1-module
creator: DTU-Denmark-2
created: 2011-09-08T11:00:00Z
modified: 2015-05-08T01:13:04Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _882_
m user id: 0
owner id: 8341
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Monomeric red fluorescent protein (mRFP1) is a derivative of the red fluorescent protein cloned from Discosoma coral (DsRed) (1). The successful use of mRFP1 in Aspergillus nidulans has previously been described (2).
mutable notes: This part was designed for the Plug'n'Play with DNA assembly standard (RFC 80), therefore this part contains tails at the 5' and 3' ends as shown on the figure below. Please notice that mRFP1-module is not the same as BBa_K678031 or BBa_K678032.
mutable provenance: From p71-mRFP1 of the Center for Microbial Biotechnology plasmid collection at the Technical University of Denmark
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: Linker 2
sequence annotation: RFP
sequence: https://synbiohub.org/public/igem/BBa_K678030_sequence/1

Linker 2
type: SequenceAnnotation
persistent identity: annotation2127388
display id: annotation2127388
version: 1
top level: mRFP1
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K678030/annotation2127388/range2127388/1

https://synbiohub.org/public/igem/BBa_K678030/annotation2127388/range2127388/1
type: Range
persistent identity: range2127388
display id: range2127388
version: 1
direction: direction/forward
top level: mRFP1
start: 1
end: 8
orientation: inline

RFP
type: SequenceAnnotation
persistent identity: annotation2127389
display id: annotation2127389
version: 1
top level: mRFP1
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K678030/annotation2127389/range2127389/1

https://synbiohub.org/public/igem/BBa_K678030/annotation2127389/range2127389/1
type: Range
persistent identity: range2127389
display id: range2127389
version: 1
direction: direction/forward
top level: mRFP1
start: 9
end: 686
orientation: inline

U085O11+D0
type: ComponentDefinition
persistent identity: BBa I732422
display id: BBa_I732422
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Family Member (U085O11+D038O22)
creator: Zhan Jian
created: 2007-10-13T11:00:00Z
modified: 2015-08-31T04:07:58Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732422_sequence/1

BBa_M13103
type: ComponentDefinition
persistent identity: BBa M13103
display id: BBa_M13103
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: M13K07 gene III promoter
creator: Natalie Kuldell
created: 2006-12-15T12:00:00Z
modified: 2015-05-08T01:13:55Z
discontinued: false
dominant: true
group u list: _45_
m user id: 0
owner id: 314
owning group id: 1
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This part is from the bacteriophage M13 genome, bp 1500-1547 in M13K07. It directs transcription of M13 gene III (BBa_M13503, BBa_M13003). Its identity as a promoter was described in Gene (1980) 11:129-148 based on -10,-35 homology and fragment of genome able to bind RNAP.
mutable notes: none
mutable provenance: New England Biolabs M13K07. Sequenced in 2002 by L. Panganaban.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_M13103_sequence/1

lux pR
type: ComponentDefinition
persistent identity: BBa R1062
display id: BBa_R1062
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter, Standard (luxR and HSL regulated -- lux pR)
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr Drew Endy
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:15Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: [Note: This is the same part as R0062 except that the -10 and -35 sites and spacing have been changed to comply with BBa_S0001]. Promoter activated by LuxR in concert with HSL The lux cassette of V. fischeri contains a left and a right promoter. The right promoter gives weak constitutive expression of downstream genes.This expression is up-regulated by the action of the LuxR activator protein complexed with the autoinducer, 3-oxo-hexanoyl-HSL. Two molecules of LuxR protein form a complex with two molecules of the signalling compound homoserine lactone (HSL). This complex binds to a palindromic site on the promoter, increasing the rate of transcription.
mutable notes: This promoter is based on the Vibrio fischeri quorum sensing gene promoters. Two genes LuxI and LuxR and transcribed in opposite directions as shown below. The original sequence from which the parts <bb_part>BBa_R0062</bb_part> and <bb_part>BBa_R0063</bb_part> were derived is shown in the picture below. <img src="<bb_file part=BBa_R0062>Image1.gif</bb_file>" width="614" height="362"> Modified to comply with BBa_S0001: TTGACA-17N-GATACT
mutable provenance: V. fischeri
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: BBa_R1062
sequence annotation: start
sequence annotation: -35
sequence annotation: LuxR/HSL
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R1062_sequence/1

BBa_R1062
type: SequenceAnnotation
persistent identity: annotation7077
display id: annotation7077
version: 1
top level: lux pR
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_R1062/annotation7077/range7077/1

https://synbiohub.org/public/igem/BBa_R1062/annotation7077/range7077/1
type: Range
persistent identity: range7077
display id: range7077
version: 1
direction: direction/forward
top level: lux pR
start: 1
end: 56
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2100
display id: annotation2100
version: 1
top level: lux pR
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_R1062/annotation2100/range2100/1

https://synbiohub.org/public/igem/BBa_R1062/annotation2100/range2100/1
type: Range
persistent identity: range2100
display id: range2100
version: 1
direction: direction/forward
top level: lux pR
start: 54
end: 54
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2101
display id: annotation2101
version: 1
top level: lux pR
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R1062/annotation2101/range2101/1

https://synbiohub.org/public/igem/BBa_R1062/annotation2101/range2101/1
type: Range
persistent identity: range2101
display id: range2101
version: 1
direction: direction/forward
top level: lux pR
start: 20
end: 25
orientation: inline

LuxR/HSL
type: SequenceAnnotation
persistent identity: annotation2098
display id: annotation2098
version: 1
top level: lux pR
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R1062/annotation2098/range2098/1

https://synbiohub.org/public/igem/BBa_R1062/annotation2098/range2098/1
type: Range
persistent identity: range2098
display id: range2098
version: 1
direction: direction/forward
top level: lux pR
start: 1
end: 20
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2099
display id: annotation2099
version: 1
top level: lux pR
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R1062/annotation2099/range2099/1

https://synbiohub.org/public/igem/BBa_R1062/annotation2099/range2099/1
type: Range
persistent identity: range2099
display id: range2099
version: 1
direction: direction/forward
top level: lux pR
start: 42
end: 48
orientation: inline

BBa_K1390019
type: ComponentDefinition
persistent identity: BBa K1390019
display id: BBa_K1390019
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Complete sMMO of Methylococcus capsulatus
creator: Torsten Meyer
created: 2014-09-03T11:00:00Z
modified: 2015-05-08T01:10:14Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1767_
m user id: 0
owner id: 17096
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: The part consists of the six subunits of methane monooxygenase (MMO). Gene order: mmoX, mmoY, mmoB, mmoZ, mmoD, mmoC The expression is under control of lac+pl. MMO converts methane to methanol.
mutable notes: Part is designed considering the RFC10 standard.
mutable provenance: Methylococcus capsulatus strain Bath
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0032
component: BBa_K1390004
component: BBa_K1390003
component: BBa_K1390001
component: BBa_R0011
component: BBa_K1390002
component: BBa_B0015
component: BBa_K1390006
component: BBa_K1390005
sequence annotation: BBa_B0032
sequence annotation: BBa_K1390006
sequence annotation: BBa_R0011
sequence annotation: BBa_B0015
sequence annotation: BBa_K1390001
sequence annotation: BBa_K1390005
sequence annotation: BBa_K1390002
sequence annotation: BBa_K1390004
sequence annotation: BBa_K1390003
sequence: https://synbiohub.org/public/igem/BBa_K1390019_sequence/1

BBa_B0032
type: Component
persistent identity: component2412367
display id: component2412367
version: 1
top level: BBa_K1390019
access: public
definition: BBa_B0032

BBa_K1390004
type: Component
persistent identity: component2412357
display id: component2412357
version: 1
top level: BBa_K1390019
access: public
definition: https://synbiohub.org/public/igem/BBa_K1390004/1

BBa_B0032
type: Component
persistent identity: component2412355
display id: component2412355
version: 1
top level: BBa_K1390019
access: public
definition: BBa_B0032

BBa_K1390003
type: Component
persistent identity: component2412373
display id: component2412373
version: 1
top level: BBa_K1390019
access: public
definition: https://synbiohub.org/public/igem/BBa_K1390003/1

BBa_K1390001
type: Component
persistent identity: component2412365
display id: component2412365
version: 1
top level: BBa_K1390019
access: public
definition: https://synbiohub.org/public/igem/BBa_K1390001/1

BBa_B0032
type: Component
persistent identity: component2412359
display id: component2412359
version: 1
top level: BBa_K1390019
access: public
definition: BBa_B0032

BBa_R0011
type: Component
persistent identity: component2412349
display id: component2412349
version: 1
top level: BBa_K1390019
access: public
definition: lacI+pL

BBa_K1390002
type: Component
persistent identity: component2412377
display id: component2412377
version: 1
top level: BBa_K1390019
access: public
definition: https://synbiohub.org/public/igem/BBa_K1390002/1

BBa_B0015
type: Component
persistent identity: component2412384
display id: component2412384
version: 1
top level: BBa_K1390019
access: public
definition: BBa_B0015

BBa_B0032
type: Component
persistent identity: component2412363
display id: component2412363
version: 1
top level: BBa_K1390019
access: public
definition: BBa_B0032

BBa_K1390006
type: Component
persistent identity: component2412369
display id: component2412369
version: 1
top level: BBa_K1390019
access: public
definition: https://synbiohub.org/public/igem/BBa_K1390006/1

BBa_K1390005
type: Component
persistent identity: component2412361
display id: component2412361
version: 1
top level: BBa_K1390019
access: public
definition: https://synbiohub.org/public/igem/BBa_K1390005/1

BBa_B0032
type: Component
persistent identity: component2412371
display id: component2412371
version: 1
top level: BBa_K1390019
access: public
definition: BBa_B0032

BBa_B0032
type: Component
persistent identity: component2412375
display id: component2412375
version: 1
top level: BBa_K1390019
access: public
definition: BBa_B0032

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation2412367
display id: annotation2412367
version: 1
top level: BBa_K1390019
location: https://synbiohub.org/public/igem/BBa_K1390019/annotation2412367/range2412367/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_K1390019/annotation2412367/range2412367/1
type: Range
persistent identity: range2412367
display id: range2412367
version: 1
direction: direction/forward
top level: BBa_K1390019
start: 3325
end: 3337
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation2412371
display id: annotation2412371
version: 1
top level: BBa_K1390019
location: https://synbiohub.org/public/igem/BBa_K1390019/annotation2412371/range2412371/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_K1390019/annotation2412371/range2412371/1
type: Range
persistent identity: range2412371
display id: range2412371
version: 1
direction: direction/forward
top level: BBa_K1390019
start: 3862
end: 3874
orientation: inline

BBa_K1390006
type: SequenceAnnotation
persistent identity: annotation2412369
display id: annotation2412369
version: 1
top level: BBa_K1390019
location: https://synbiohub.org/public/igem/BBa_K1390019/annotation2412369/range2412369/1
component: BBa_K1390006

https://synbiohub.org/public/igem/BBa_K1390019/annotation2412369/range2412369/1
type: Range
persistent identity: range2412369
display id: range2412369
version: 1
direction: direction/reverse
top level: BBa_K1390019
start: 3344
end: 3853
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation2412375
display id: annotation2412375
version: 1
top level: BBa_K1390019
location: https://synbiohub.org/public/igem/BBa_K1390019/annotation2412375/range2412375/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_K1390019/annotation2412375/range2412375/1
type: Range
persistent identity: range2412375
display id: range2412375
version: 1
direction: direction/forward
top level: BBa_K1390019
start: 4201
end: 4213
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation2412355
display id: annotation2412355
version: 1
top level: BBa_K1390019
location: https://synbiohub.org/public/igem/BBa_K1390019/annotation2412355/range2412355/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_K1390019/annotation2412355/range2412355/1
type: Range
persistent identity: range2412355
display id: range2412355
version: 1
direction: direction/forward
top level: BBa_K1390019
start: 64
end: 76
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation2412363
display id: annotation2412363
version: 1
top level: BBa_K1390019
location: https://synbiohub.org/public/igem/BBa_K1390019/annotation2412363/range2412363/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_K1390019/annotation2412363/range2412363/1
type: Range
persistent identity: range2412363
display id: range2412363
version: 1
direction: direction/forward
top level: BBa_K1390019
start: 2872
end: 2884
orientation: inline

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation2412349
display id: annotation2412349
version: 1
top level: BBa_K1390019
location: https://synbiohub.org/public/igem/BBa_K1390019/annotation2412349/range2412349/1
component: BBa_R0011

https://synbiohub.org/public/igem/BBa_K1390019/annotation2412349/range2412349/1
type: Range
persistent identity: range2412349
display id: range2412349
version: 1
direction: direction/forward
top level: BBa_K1390019
start: 1
end: 54
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation2412359
display id: annotation2412359
version: 1
top level: BBa_K1390019
location: https://synbiohub.org/public/igem/BBa_K1390019/annotation2412359/range2412359/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_K1390019/annotation2412359/range2412359/1
type: Range
persistent identity: range2412359
display id: range2412359
version: 1
direction: direction/forward
top level: BBa_K1390019
start: 1675
end: 1687
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2412384
display id: annotation2412384
version: 1
top level: BBa_K1390019
location: https://synbiohub.org/public/igem/BBa_K1390019/annotation2412384/range2412384/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K1390019/annotation2412384/range2412384/1
type: Range
persistent identity: range2412384
display id: range2412384
version: 1
direction: direction/reverse
top level: BBa_K1390019
start: 5275
end: 5403
orientation: inline

BBa_K1390001
type: SequenceAnnotation
persistent identity: annotation2412365
display id: annotation2412365
version: 1
top level: BBa_K1390019
location: https://synbiohub.org/public/igem/BBa_K1390019/annotation2412365/range2412365/1
component: BBa_K1390001

https://synbiohub.org/public/igem/BBa_K1390019/annotation2412365/range2412365/1
type: Range
persistent identity: range2412365
display id: range2412365
version: 1
direction: direction/reverse
top level: BBa_K1390019
start: 2891
end: 3316
orientation: inline

BBa_K1390005
type: SequenceAnnotation
persistent identity: annotation2412361
display id: annotation2412361
version: 1
top level: BBa_K1390019
location: https://synbiohub.org/public/igem/BBa_K1390019/annotation2412361/range2412361/1
component: BBa_K1390005

https://synbiohub.org/public/igem/BBa_K1390019/annotation2412361/range2412361/1
type: Range
persistent identity: range2412361
display id: range2412361
version: 1
direction: direction/reverse
top level: BBa_K1390019
start: 1694
end: 2863
orientation: inline

BBa_K1390002
type: SequenceAnnotation
persistent identity: annotation2412377
display id: annotation2412377
version: 1
top level: BBa_K1390019
location: https://synbiohub.org/public/igem/BBa_K1390019/annotation2412377/range2412377/1
component: BBa_K1390002

https://synbiohub.org/public/igem/BBa_K1390019/annotation2412377/range2412377/1
type: Range
persistent identity: range2412377
display id: range2412377
version: 1
direction: direction/reverse
top level: BBa_K1390019
start: 4220
end: 5266
orientation: inline

BBa_K1390004
type: SequenceAnnotation
persistent identity: annotation2412357
display id: annotation2412357
version: 1
top level: BBa_K1390019
location: https://synbiohub.org/public/igem/BBa_K1390019/annotation2412357/range2412357/1
component: BBa_K1390004

https://synbiohub.org/public/igem/BBa_K1390019/annotation2412357/range2412357/1
type: Range
persistent identity: range2412357
display id: range2412357
version: 1
direction: direction/reverse
top level: BBa_K1390019
start: 83
end: 1666
orientation: inline

BBa_K1390003
type: SequenceAnnotation
persistent identity: annotation2412373
display id: annotation2412373
version: 1
top level: BBa_K1390019
location: https://synbiohub.org/public/igem/BBa_K1390019/annotation2412373/range2412373/1
component: BBa_K1390003

https://synbiohub.org/public/igem/BBa_K1390019/annotation2412373/range2412373/1
type: Range
persistent identity: range2412373
display id: range2412373
version: 1
direction: direction/reverse
top level: BBa_K1390019
start: 3881
end: 4192
orientation: inline

BBa_K112401
type: ComponentDefinition
persistent identity: BBa K112401
display id: BBa_K112401
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter for recA gene - SOS and Ultrasound Sensitive
creator: Christina Brown
created: 2008-10-20T11:00:00Z
modified: 2015-05-08T01:09:17Z
discontinued: false
dominant: false
group u list: _224_
m user id: 0
owner id: 3241
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: According to Vollmer, et. al in their 1998 Applied and Enviormental Microbiology paper, "Bacterial Stress Responses to 1-Megahertz Pulsed Ultrasound in the Presence of Microbubble", the promoter for the recA gene, a known SOS response gene, is upregulated 4 fold when cells are exposed to high frequency ultrasound and microbubble cavitation.
mutable notes: We did not see the desired response from ultrasound exposure that we expected from this promoter. However, we did not induce the same acoustic cavitation that Vollmer et. al. used to elicit a response from this promoter.
mutable provenance: Cloned from E. coli MG1655 genome.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K112401_sequence/1

BBa_K090506
type: ComponentDefinition
persistent identity: BBa K090506
display id: BBa_K090506
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: ''Bacillus subtilis'' weak RBS
creator: Daniel Goodman
created: 2008-10-27T12:00:00Z
modified: 2015-05-08T01:08:37Z
discontinued: false
dominant: false
experience: experience/None
group u list: _188_
m user id: 0
owner id: 3501
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is the weak version of the consensus RBS for Bacillus subtilis. The sequence is present in front of some B. subtilis operons, but is expected to give weaker expression than the consensus sequence. The consensus sequence AAAGGAGG (BBa_K090505) was changed to AGAGGTGG for this part.
mutable notes: The part must be 8 nucleotides away from the first methionine of the coding sequence, and so we added 'TGT' downstream of the actual binding sequence: AGAGGTGG. This, combined with the truncated non-CDS BioBrick prefix scar, gives exactly 8 'spacer bases' between the 3' end of the RBS and the start of the coding sequence.
mutable provenance: This part was synthesized directly.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: B. subtilis weak RBS
sequence: https://synbiohub.org/public/igem/BBa_K090506_sequence/1

B. subtilis weak RBS
type: SequenceAnnotation
persistent identity: annotation1992815
display id: annotation1992815
version: 1
top level: BBa_K090506
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K090506/annotation1992815/range1992815/1

https://synbiohub.org/public/igem/BBa_K090506/annotation1992815/range1992815/1
type: Range
persistent identity: range1992815
display id: range1992815
version: 1
direction: direction/forward
top level: BBa_K090506
start: 1
end: 8
orientation: inline

BBa_K112405
type: ComponentDefinition
persistent identity: BBa K112405
display id: BBa_K112405
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter for CadA and CadB genes
creator: Christina Brown
created: 2008-10-20T11:00:00Z
modified: 2015-05-08T01:09:17Z
discontinued: false
dominant: false
group u list: _224_
m user id: 0
owner id: 3241
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Our microarray experiment showed that this gene was responsive to sound and ultrasound. Therefore, we cloned the promoter region located approximately 250 base pairs before the start codon for the cadB gene.
mutable notes: There was a relatively high basal level of expression of this gene that made this part difficult to incorporate into our lysis device design.
mutable provenance: cloned from E. coli MG1655 genome located upstream of CadA and CadB genes
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K112405_sequence/1

BBa_I732021
type: ComponentDefinition
persistent identity: BBa I732021
display id: BBa_I732021
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Template for Building Primer Family Member
creator: Zhan Jian
created: 2007-07-26T11:00:00Z
modified: 2015-08-31T04:07:56Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1572
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: +1
sequence annotation: -35
sequence annotation: -10
sequence annotation: consensus elements
sequence: https://synbiohub.org/public/igem/BBa_I732021_sequence/1

+1
type: SequenceAnnotation
persistent identity: annotation1939929
display id: annotation1939929
version: 1
top level: BBa_I732021
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I732021/annotation1939929/range1939929/1

https://synbiohub.org/public/igem/BBa_I732021/annotation1939929/range1939929/1
type: Range
persistent identity: range1939929
display id: range1939929
version: 1
direction: direction/forward
top level: BBa_I732021
start: 98
end: 98
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1939927
display id: annotation1939927
version: 1
top level: BBa_I732021
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I732021/annotation1939927/range1939927/1

https://synbiohub.org/public/igem/BBa_I732021/annotation1939927/range1939927/1
type: Range
persistent identity: range1939927
display id: range1939927
version: 1
direction: direction/forward
top level: BBa_I732021
start: 62
end: 67
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1939928
display id: annotation1939928
version: 1
top level: BBa_I732021
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I732021/annotation1939928/range1939928/1

https://synbiohub.org/public/igem/BBa_I732021/annotation1939928/range1939928/1
type: Range
persistent identity: range1939928
display id: range1939928
version: 1
direction: direction/forward
top level: BBa_I732021
start: 86
end: 91
orientation: inline

consensus elements
type: SequenceAnnotation
persistent identity: annotation1939930
display id: annotation1939930
version: 1
top level: BBa_I732021
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_I732021/annotation1939930/range1939930/1

https://synbiohub.org/public/igem/BBa_I732021/annotation1939930/range1939930/1
type: Range
persistent identity: range1939930
display id: range1939930
version: 1
direction: direction/forward
top level: BBa_I732021
start: 62
end: 98
orientation: inline

BBa_K165031
type: ComponentDefinition
persistent identity: BBa K165031
display id: BBa_K165031
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: mCYC promoter plus LexA binding sites
creator: Aaron Glieberman
created: 2008-10-28T12:00:00Z
modified: 2015-05-08T01:10:56Z
discontinued: false
dominant: false
group u list: _267_
m user id: 0
owner id: 2510
owning group id: 58
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This is a composite part containing the minimal promoter mCYC preceded by multiple consecutive binding sites for the LexA binding domain.
mutable notes: It was necessary to design primers for use in PCR to remove this part from its host plasmid and transfer it to a standard Biobrick vector
mutable provenance: Composite part was taken via PCR from a synthetic plasmid containing the sequence. The plasmid came from Lawrence Berkeley National Laboratory.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K165031_sequence/1

BBa_K091104
type: ComponentDefinition
persistent identity: BBa K091104
display id: BBa_K091104
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pLac/Mnt Hybrid Promoter
creator: Robert Cool
created: 2008-06-18T11:00:00Z
modified: 2015-05-08T01:08:37Z
discontinued: false
dominant: false
experience: experience/None
group u list: _191_
m user id: 0
owner id: 3080
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This promoter is a modified version of the Mnt promoter that is also responsive to LacI. The promoter should be repressed by Mnt repressor. It should also be repressed by LacI, and in the absence of Mnt repressor, should be induced by IPTG.
mutable notes: Mnt repressor binds as a tetramer to two half-operator sites. Introduction of a LacI binding site between the Mnt promoter -10 sequence and the start site for transcription should allow for repression by LacI. Accordingly, the hybrid promoter was designed by 1) Remove all bases of mnt promoter 3??? to -10: gagtcgtattaattt will be replaced by tgtgtggaattgtga, and 2) Position lacI binding site (composed of an inverted repeat) from the lacI regulated promoter (R0010) immediately 3??? to truncated mnt promoter.
mutable provenance: This part comes from the Mnt promoter and the LacI promoter
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: LacI
sequence annotation: Mnt
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_K091104_sequence/1

LacI
type: SequenceAnnotation
persistent identity: annotation1963738
display id: annotation1963738
version: 1
top level: BBa_K091104
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K091104/annotation1963738/range1963738/1

https://synbiohub.org/public/igem/BBa_K091104/annotation1963738/range1963738/1
type: Range
persistent identity: range1963738
display id: range1963738
version: 1
direction: direction/forward
top level: BBa_K091104
start: 53
end: 87
orientation: inline

Mnt
type: SequenceAnnotation
persistent identity: annotation1963743
display id: annotation1963743
version: 1
top level: BBa_K091104
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K091104/annotation1963743/range1963743/1

https://synbiohub.org/public/igem/BBa_K091104/annotation1963743/range1963743/1
type: Range
persistent identity: range1963743
display id: range1963743
version: 1
direction: direction/forward
top level: BBa_K091104
start: 1
end: 52
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1963737
display id: annotation1963737
version: 1
top level: BBa_K091104
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K091104/annotation1963737/range1963737/1

https://synbiohub.org/public/igem/BBa_K091104/annotation1963737/range1963737/1
type: Range
persistent identity: range1963737
display id: range1963737
version: 1
direction: direction/forward
top level: BBa_K091104
start: 47
end: 52
orientation: inline

BBa_K086026
type: ComponentDefinition
persistent identity: BBa K086026
display id: BBa_K086026
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: modified Lutz-Bujard LacO promoter,with alternative sigma factor σ32
creator: Gairik Sachdeva
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:08:37Z
discontinued: false
dominant: false
group u list: _197_
m user id: 0
owner id: 2578
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: modified Lutz-Bujard LacO promoter,with alternative sigma factor σ32
mutable notes: cleaned
mutable provenance: Lac O and sigma 32 binding sites conserved sequences
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: P32_1
sequence: https://synbiohub.org/public/igem/BBa_K086026_sequence/1

P32_1
type: SequenceAnnotation
persistent identity: annotation1995760
display id: annotation1995760
version: 1
top level: BBa_K086026
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K086026/annotation1995760/range1995760/1

https://synbiohub.org/public/igem/BBa_K086026/annotation1995760/range1995760/1
type: Range
persistent identity: range1995760
display id: range1995760
version: 1
direction: direction/forward
top level: BBa_K086026
start: 1
end: 55
orientation: inline

BBa_K086029
type: ComponentDefinition
persistent identity: BBa K086029
display id: BBa_K086029
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: modified Lutz-Bujard LacO promoter,with alternative sigma factor σ32
creator: Gairik Sachdeva
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:08:37Z
discontinued: false
dominant: false
group u list: _197_
m user id: 0
owner id: 2578
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: modified Lutz-Bujard LacO promoter,with alternative sigma factor σ32
mutable notes: cleaned
mutable provenance: Lac O and sigma 32 binding sites conserved sequences
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: P32_4
sequence: https://synbiohub.org/public/igem/BBa_K086029_sequence/1

P32_4
type: SequenceAnnotation
persistent identity: annotation1995763
display id: annotation1995763
version: 1
top level: BBa_K086029
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K086029/annotation1995763/range1995763/1

https://synbiohub.org/public/igem/BBa_K086029/annotation1995763/range1995763/1
type: Range
persistent identity: range1995763
display id: range1995763
version: 1
direction: direction/forward
top level: BBa_K086029
start: 1
end: 55
orientation: inline

BBa_K314000
type: ComponentDefinition
persistent identity: BBa K314000
display id: BBa_K314000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: testing, 123
creator: Michal Galdzicki, Jeff Johnson
created: 2010-06-20T11:00:00Z
modified: 2015-05-08T01:11:54Z
discontinued: true
dominant: false
experience: experience/None
group u list: _496_
m user id: 0
owner id: 5533
owning group id: 9
sample status: Discontinued
status: status/Deleted
bookmark: false
mutable description: test1
mutable notes: test
mutable provenance: testing
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000110
role: partType/Temporary
sequence annotation: yy
sequence annotation: tester feature
sequence annotation: BBa_K314000
sequence: https://synbiohub.org/public/igem/BBa_K314000_sequence/1

yy
type: SequenceAnnotation
persistent identity: annotation2073213
display id: annotation2073213
version: 1
top level: BBa_K314000
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K314000/annotation2073213/range2073213/1

https://synbiohub.org/public/igem/BBa_K314000/annotation2073213/range2073213/1
type: Range
persistent identity: range2073213
display id: range2073213
version: 1
direction: direction/forward
top level: BBa_K314000
start: 5
end: 6
orientation: inline

tester feature
type: SequenceAnnotation
persistent identity: annotation2073210
display id: annotation2073210
version: 1
top level: BBa_K314000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K314000/annotation2073210/range2073210/1

https://synbiohub.org/public/igem/BBa_K314000/annotation2073210/range2073210/1
type: Range
persistent identity: range2073210
display id: range2073210
version: 1
direction: direction/forward
top level: BBa_K314000
start: 1
end: 6
orientation: inline

BBa_K314000
type: SequenceAnnotation
persistent identity: annotation2073214
display id: annotation2073214
version: 1
top level: BBa_K314000
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K314000/annotation2073214/range2073214/1

https://synbiohub.org/public/igem/BBa_K314000/annotation2073214/range2073214/1
type: Range
persistent identity: range2073214
display id: range2073214
version: 1
direction: direction/reverse
top level: BBa_K314000
start: 1
end: 76
orientation: inline

BBa_K1486041
type: ComponentDefinition
persistent identity: BBa K1486041
display id: BBa_K1486041
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: sfGFPC + CpxR
creator: Ione Pla Puigsubir??
created: 2014-09-26T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: To edit
mutable notes: To edit
mutable provenance: To edit
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0034
component: BBa_K1486037
component: BBa_K1486039
component: BBa_K1486000
component: BBa_I0500
sequence annotation: BBa_K1486039
sequence annotation: BBa_B0034
sequence annotation: BBa_K1486000
sequence annotation: BBa_K1486037
sequence annotation: BBa_I0500
sequence: https://synbiohub.org/public/igem/BBa_K1486041_sequence/1

BBa_B0034
type: Component
persistent identity: component2387211
display id: component2387211
version: 1
top level: BBa_K1486041
access: public
definition: BBa_B0034

BBa_K1486037
type: Component
persistent identity: component2387213
display id: component2387213
version: 1
top level: BBa_K1486041
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486037/1

BBa_K1486039
type: Component
persistent identity: component2387212
display id: component2387212
version: 1
top level: BBa_K1486041
access: public
definition: BBa_K1486039

BBa_K1486000
type: Component
persistent identity: component2387217
display id: component2387217
version: 1
top level: BBa_K1486041
access: public
definition: BBa_K1486000

BBa_I0500
type: Component
persistent identity: component2387209
display id: component2387209
version: 1
top level: BBa_K1486041
access: public
definition: pBad/araC

BBa_K1486039
type: SequenceAnnotation
persistent identity: annotation2387212
display id: annotation2387212
version: 1
top level: BBa_K1486041
location: https://synbiohub.org/public/igem/BBa_K1486041/annotation2387212/range2387212/1
component: BBa_K1486039

https://synbiohub.org/public/igem/BBa_K1486041/annotation2387212/range2387212/1
type: Range
persistent identity: range2387212
display id: range2387212
version: 1
direction: direction/reverse
top level: BBa_K1486041
start: 1223
end: 1294
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2387211
display id: annotation2387211
version: 1
top level: BBa_K1486041
location: https://synbiohub.org/public/igem/BBa_K1486041/annotation2387211/range2387211/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1486041/annotation2387211/range2387211/1
type: Range
persistent identity: range2387211
display id: range2387211
version: 1
direction: direction/reverse
top level: BBa_K1486041
start: 1211
end: 1222
orientation: inline

BBa_K1486000
type: SequenceAnnotation
persistent identity: annotation2387217
display id: annotation2387217
version: 1
top level: BBa_K1486041
location: https://synbiohub.org/public/igem/BBa_K1486041/annotation2387217/range2387217/1
component: BBa_K1486000

https://synbiohub.org/public/igem/BBa_K1486041/annotation2387217/range2387217/1
type: Range
persistent identity: range2387217
display id: range2387217
version: 1
direction: direction/reverse
top level: BBa_K1486041
start: 1334
end: 2035
orientation: inline

BBa_K1486037
type: SequenceAnnotation
persistent identity: annotation2387213
display id: annotation2387213
version: 1
top level: BBa_K1486041
location: https://synbiohub.org/public/igem/BBa_K1486041/annotation2387213/range2387213/1
component: BBa_K1486037

https://synbiohub.org/public/igem/BBa_K1486041/annotation2387213/range2387213/1
type: Range
persistent identity: range2387213
display id: range2387213
version: 1
direction: direction/reverse
top level: BBa_K1486041
start: 1295
end: 1333
orientation: inline

BBa_I0500
type: SequenceAnnotation
persistent identity: annotation2387209
display id: annotation2387209
version: 1
top level: BBa_K1486041
location: https://synbiohub.org/public/igem/BBa_K1486041/annotation2387209/range2387209/1
component: BBa_I0500

https://synbiohub.org/public/igem/BBa_K1486041/annotation2387209/range2387209/1
type: Range
persistent identity: range2387209
display id: range2387209
version: 1
direction: direction/reverse
top level: BBa_K1486041
start: 1
end: 1210
orientation: inline

BBa_K091107
type: ComponentDefinition
persistent identity: BBa K091107
display id: BBa_K091107
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pLux/cI Hybrid Promoter
creator: Xiao Zhu
created: 2008-06-18T11:00:00Z
modified: 2015-05-08T01:08:37Z
discontinued: false
dominant: false
experience: experience/None
group u list: _191_
m user id: 0
owner id: 3130
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This hybrid promoter is designed to respond to activation by LuxR when it is bound to AHL and to repression by the phage lambda cI repressor. The repression needs to win over activation.
mutable notes: This part is based on the idea of replacing the region of the Lux promoter downstream of the -35 with the OR2 cI half-operator, added LuxR-10 region. Based on what we have found up to now, promoters which are constructed just by inserting OR1/2 downstream of Lux are all very leaky.
mutable provenance: this part comes from LuxR promoter and cI lambda promoter.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: Lux -35
sequence annotation: Lux Box
sequence annotation: Lux -10
sequence annotation: cI OR2
sequence: https://synbiohub.org/public/igem/BBa_K091107_sequence/1

Lux -35
type: SequenceAnnotation
persistent identity: annotation1963751
display id: annotation1963751
version: 1
top level: BBa_K091107
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K091107/annotation1963751/range1963751/1

https://synbiohub.org/public/igem/BBa_K091107/annotation1963751/range1963751/1
type: Range
persistent identity: range1963751
display id: range1963751
version: 1
direction: direction/forward
top level: BBa_K091107
start: 20
end: 25
orientation: inline

Lux Box
type: SequenceAnnotation
persistent identity: annotation1963750
display id: annotation1963750
version: 1
top level: BBa_K091107
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K091107/annotation1963750/range1963750/1

https://synbiohub.org/public/igem/BBa_K091107/annotation1963750/range1963750/1
type: Range
persistent identity: range1963750
display id: range1963750
version: 1
direction: direction/forward
top level: BBa_K091107
start: 1
end: 20
orientation: inline

Lux -10
type: SequenceAnnotation
persistent identity: annotation1963753
display id: annotation1963753
version: 1
top level: BBa_K091107
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K091107/annotation1963753/range1963753/1

https://synbiohub.org/public/igem/BBa_K091107/annotation1963753/range1963753/1
type: Range
persistent identity: range1963753
display id: range1963753
version: 1
direction: direction/forward
top level: BBa_K091107
start: 44
end: 49
orientation: inline

cI OR2
type: SequenceAnnotation
persistent identity: annotation1963752
display id: annotation1963752
version: 1
top level: BBa_K091107
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K091107/annotation1963752/range1963752/1

https://synbiohub.org/public/igem/BBa_K091107/annotation1963752/range1963752/1
type: Range
persistent identity: range1963752
display id: range1963752
version: 1
direction: direction/forward
top level: BBa_K091107
start: 26
end: 43
orientation: inline

BBa_K149001
type: ComponentDefinition
persistent identity: BBa K149001
display id: BBa_K149001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Prp22 promoter
creator: Chris Euler
created: 2008-08-14T11:00:00Z
modified: 2015-05-08T01:10:44Z
discontinued: false
dominant: false
group u list: _243_
m user id: 0
owner id: 3441
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Ubiquitous strong promoter.
mutable notes: None
mutable provenance: Simon St. Pierre extracted from S. Cervisiae genome.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K149001_sequence/1

BBa_I742159
type: ComponentDefinition
persistent identity: BBa I742159
display id: BBa_I742159
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: crtI native rbs
creator: Chris French
created: 2007-10-23T11:00:00Z
modified: 2015-08-31T04:08:03Z
discontinued: false
dominant: false
experience: experience/None
group u list: _123_
m user id: 0
owner id: 837
owning group id: 163
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Native rbs from crtI (phytoene dehydrogenase) of Pantoea ananatis (formerly Erwinia uredovora) DSMZ 30080 (ATCC 19321). Accession: D90087.
mutable notes: Virtual part to be added to the coding sequence.
mutable provenance: Pantoea ananatis (formerly Erwinia uredovora) DSMZ 30080 (ATCC 19321). Accession: D90087.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: rbs
sequence: https://synbiohub.org/public/igem/BBa_I742159_sequence/1

rbs
type: SequenceAnnotation
persistent identity: annotation1953816
display id: annotation1953816
version: 1
top level: BBa_I742159
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_I742159/annotation1953816/range1953816/1

https://synbiohub.org/public/igem/BBa_I742159/annotation1953816/range1953816/1
type: Range
persistent identity: range1953816
display id: range1953816
version: 1
direction: direction/forward
top level: BBa_I742159
start: 10
end: 15
orientation: inline

BBa_K086030
type: ComponentDefinition
persistent identity: BBa K086030
display id: BBa_K086030
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: modified Lutz-Bujard LacO promoter,with alternative sigma factor σ38
creator: Gairik Sachdeva
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:08:37Z
discontinued: false
dominant: false
group u list: _197_
m user id: 0
owner id: 2578
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: modified Lutz-Bujard LacO promoter,with alternative sigma factor σ38
mutable notes: cleaned
mutable provenance: Lac O and sigma 38 binding sites conserved sequences
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: P38_1
sequence: https://synbiohub.org/public/igem/BBa_K086030_sequence/1

P38_1
type: SequenceAnnotation
persistent identity: annotation1995764
display id: annotation1995764
version: 1
top level: BBa_K086030
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K086030/annotation1995764/range1995764/1

https://synbiohub.org/public/igem/BBa_K086030/annotation1995764/range1995764/1
type: Range
persistent identity: range1995764
display id: range1995764
version: 1
direction: direction/forward
top level: BBa_K086030
start: 1
end: 55
orientation: inline

U085O11+D0
type: ComponentDefinition
persistent identity: BBa I732420
display id: BBa_I732420
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Family Member (U085O11+D014O22)
creator: Zhan Jian
created: 2007-10-13T11:00:00Z
modified: 2015-08-31T04:07:58Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732420_sequence/1

BBa_K206001
type: ComponentDefinition
persistent identity: BBa K206001
display id: BBa_K206001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pBAD weak
creator: Amelia Hardjasa
created: 2009-10-16T11:00:00Z
modified: 2015-05-08T01:11:23Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _307_
m user id: 0
owner id: 4172
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: Weaker version of wild type pBAD ( I13453 ).
mutable notes: No special considerations
mutable provenance: Site-directed mutagenesis on I13453 with the following primers: Forward: TAATCTTATGGATAAAAAAGCTATGGCATAGC Reverse: GCGGATCCTACCTGACGCTTTTTATC
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: promoter
sequence annotation: AraI2
sequence annotation: AraI1
sequence: https://synbiohub.org/public/igem/BBa_K206001_sequence/1

promoter
type: SequenceAnnotation
persistent identity: annotation2049255
display id: annotation2049255
version: 1
top level: BBa_K206001
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K206001/annotation2049255/range2049255/1

https://synbiohub.org/public/igem/BBa_K206001/annotation2049255/range2049255/1
type: Range
persistent identity: range2049255
display id: range2049255
version: 1
direction: direction/forward
top level: BBa_K206001
start: 1
end: 131
orientation: inline

AraI2
type: SequenceAnnotation
persistent identity: annotation2049257
display id: annotation2049257
version: 1
top level: BBa_K206001
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K206001/annotation2049257/range2049257/1

https://synbiohub.org/public/igem/BBa_K206001/annotation2049257/range2049257/1
type: Range
persistent identity: range2049257
display id: range2049257
version: 1
direction: direction/forward
top level: BBa_K206001
start: 61
end: 78
orientation: inline

AraI1
type: SequenceAnnotation
persistent identity: annotation2049256
display id: annotation2049256
version: 1
top level: BBa_K206001
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K206001/annotation2049256/range2049256/1

https://synbiohub.org/public/igem/BBa_K206001/annotation2049256/range2049256/1
type: Range
persistent identity: range2049256
display id: range2049256
version: 1
direction: direction/forward
top level: BBa_K206001
start: 40
end: 57
orientation: inline

BBa_K292002
type: ComponentDefinition
persistent identity: BBa K292002
display id: BBa_K292002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pLac (LacI regulated) + Strong RBS
creator: David Charoy
created: 2009-10-18T11:00:00Z
modified: 2015-05-08T01:11:49Z
discontinued: false
dominant: false
group u list: _394_
m user id: 0
owner id: 5212
owning group id: 9
sample status: In stock
sample status: It's complicated
status: status/Available
status: status/Planning
bookmark: false
mutable description: This part contains the pLac promoter and a ribosome binding site (BBa_R0010 + BBa_B0030). The Lac promoter is constitutively activated, but it can be repressed by the Lac I repressor from E.coli. This promoter is really useful to control a gene expression by inactivating it with LacI repressor. When LacI is absent the gene should be expressed, and in the presence of LacI, pLac is inhibited. So this part is really suitable for gene expression control by inactivation of pLac with LacI. We can use this part to control a failed feedback mechanism which induces the Lambda phage lytic cycle. In E.Coli, pLac is a part of the lactose operon. LacI is constantly expressed and represses the pLac. In the presence of Lactose, LacI is inhibited and pLac is active and allows the transcription of beta-galactosidase. Lactose can be substituted by IPTG (Isopropyl β-D-1-thiogalactopyranoside). This part already contains a strong RBS to begin directly a gene transcription.
mutable notes: N/A
mutable provenance: Part:BBa_R0010 Part:BBa_B0030, Designed by Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. Group: NCBS, Prather Lab.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_B0030
component: BBa_R0010
sequence annotation: BBa_R0010
sequence annotation: BBa_B0030
sequence: https://synbiohub.org/public/igem/BBa_K292002_sequence/1

BBa_B0030
type: Component
persistent identity: component2054553
display id: component2054553
version: 1
top level: BBa_K292002
access: public
definition: BBa_B0030

BBa_R0010
type: Component
persistent identity: component2054545
display id: component2054545
version: 1
top level: BBa_K292002
access: public
definition: LacI

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation2054545
display id: annotation2054545
version: 1
top level: BBa_K292002
location: https://synbiohub.org/public/igem/BBa_K292002/annotation2054545/range2054545/1
component: BBa_R0010

https://synbiohub.org/public/igem/BBa_K292002/annotation2054545/range2054545/1
type: Range
persistent identity: range2054545
display id: range2054545
version: 1
direction: direction/forward
top level: BBa_K292002
start: 1
end: 200
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation2054553
display id: annotation2054553
version: 1
top level: BBa_K292002
location: https://synbiohub.org/public/igem/BBa_K292002/annotation2054553/range2054553/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_K292002/annotation2054553/range2054553/1
type: Range
persistent identity: range2054553
display id: range2054553
version: 1
direction: direction/forward
top level: BBa_K292002
start: 209
end: 223
orientation: inline

BBa_B1003
type: ComponentDefinition
persistent identity: BBa B1003
display id: BBa_B1003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Terminator (artificial, small, %T~=80)
creator: Haiyao Huang
created: 2006-08-30T11:00:00Z
modified: 2015-08-31T04:07:21Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _41_
m user id: 0
owner id: 745
owning group id: 41
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Artifical terminator, estimated %T~=80 6bp stem, 4nt loop Bidirectional, estimated reverse %T=40
mutable notes: Bidirectional, with the reverse estimated to be less effective than the forward. Has a polyA tail of 5 residues.
mutable provenance: antiquity
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: PolyA
sequence annotation: stem loop
sequence annotation: B1003
sequence: https://synbiohub.org/public/igem/BBa_B1003_sequence/1

PolyA
type: SequenceAnnotation
persistent identity: annotation1898418
display id: annotation1898418
version: 1
top level: BBa_B1003
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_B1003/annotation1898418/range1898418/1

https://synbiohub.org/public/igem/BBa_B1003/annotation1898418/range1898418/1
type: Range
persistent identity: range1898418
display id: range1898418
version: 1
direction: direction/reverse
top level: BBa_B1003
start: 5
end: 9
orientation: inline

PolyA
type: SequenceAnnotation
persistent identity: annotation1898419
display id: annotation1898419
version: 1
top level: BBa_B1003
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_B1003/annotation1898419/range1898419/1

https://synbiohub.org/public/igem/BBa_B1003/annotation1898419/range1898419/1
type: Range
persistent identity: range1898419
display id: range1898419
version: 1
direction: direction/forward
top level: BBa_B1003
start: 26
end: 30
orientation: inline

stem loop
type: SequenceAnnotation
persistent identity: annotation1898417
display id: annotation1898417
version: 1
top level: BBa_B1003
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_B1003/annotation1898417/range1898417/1

https://synbiohub.org/public/igem/BBa_B1003/annotation1898417/range1898417/1
type: Range
persistent identity: range1898417
display id: range1898417
version: 1
direction: direction/forward
top level: BBa_B1003
start: 10
end: 25
orientation: inline

B1003
type: SequenceAnnotation
persistent identity: annotation1898416
display id: annotation1898416
version: 1
top level: BBa_B1003
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_B1003/annotation1898416/range1898416/1

https://synbiohub.org/public/igem/BBa_B1003/annotation1898416/range1898416/1
type: Range
persistent identity: range1898416
display id: range1898416
version: 1
direction: direction/forward
top level: BBa_B1003
start: 1
end: 34
orientation: inline

BBa_K180020
type: ComponentDefinition
persistent identity: BBa K180020
display id: BBa_K180020
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Rock-paper-scissors - Scissors secondary plasmid [las pR, LacI generator]
creator: Richard Wilson, Stephanie Chambers
created: 2009-10-18T11:00:00Z
modified: 2015-05-08T01:11:06Z
discontinued: false
dominant: false
experience: experience/None
group u list: _278_
m user id: 0
owner id: 4082
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: iGEM2009 Team:Southampton rock-paper-scissors 'scissors' secondary plasmid.
mutable notes: Regulated expression of LacI (C0012) by Las pR (R0079).
mutable provenance: See relevant basic biobrick parts (R0070, B0034, C0012, B0015).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0015
component: BBa_C0012
component: BBa_R0079
component: BBa_B0034
sequence annotation: BBa_R0079
sequence annotation: BBa_B0015
sequence annotation: BBa_C0012
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K180020_sequence/1

BBa_B0015
type: Component
persistent identity: component2256469
display id: component2256469
version: 1
top level: BBa_K180020
access: public
definition: BBa_B0015

BBa_C0012
type: Component
persistent identity: component2256460
display id: component2256460
version: 1
top level: BBa_K180020
access: public
definition: https://synbiohub.org/public/igem/BBa_C0012/1

BBa_R0079
type: Component
persistent identity: component2256456
display id: component2256456
version: 1
top level: BBa_K180020
access: public
definition: LasR+PAI

BBa_B0034
type: Component
persistent identity: component2256458
display id: component2256458
version: 1
top level: BBa_K180020
access: public
definition: BBa_B0034

BBa_R0079
type: SequenceAnnotation
persistent identity: annotation2256456
display id: annotation2256456
version: 1
top level: BBa_K180020
location: https://synbiohub.org/public/igem/BBa_K180020/annotation2256456/range2256456/1
component: BBa_R0079

https://synbiohub.org/public/igem/BBa_K180020/annotation2256456/range2256456/1
type: Range
persistent identity: range2256456
display id: range2256456
version: 1
direction: direction/reverse
top level: BBa_K180020
start: 1
end: 157
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2256469
display id: annotation2256469
version: 1
top level: BBa_K180020
location: https://synbiohub.org/public/igem/BBa_K180020/annotation2256469/range2256469/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K180020/annotation2256469/range2256469/1
type: Range
persistent identity: range2256469
display id: range2256469
version: 1
direction: direction/reverse
top level: BBa_K180020
start: 1345
end: 1473
orientation: inline

BBa_C0012
type: SequenceAnnotation
persistent identity: annotation2256460
display id: annotation2256460
version: 1
top level: BBa_K180020
location: https://synbiohub.org/public/igem/BBa_K180020/annotation2256460/range2256460/1
component: BBa_C0012

https://synbiohub.org/public/igem/BBa_K180020/annotation2256460/range2256460/1
type: Range
persistent identity: range2256460
display id: range2256460
version: 1
direction: direction/forward
top level: BBa_K180020
start: 184
end: 1311
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2256458
display id: annotation2256458
version: 1
top level: BBa_K180020
location: https://synbiohub.org/public/igem/BBa_K180020/annotation2256458/range2256458/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K180020/annotation2256458/range2256458/1
type: Range
persistent identity: range2256458
display id: range2256458
version: 1
direction: direction/reverse
top level: BBa_K180020
start: 166
end: 177
orientation: inline

BBa_K165001
type: ComponentDefinition
persistent identity: BBa K165001
display id: BBa_K165001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pGAL1+ w/XhoI sites
creator: John Szymanski
created: 2008-10-25T11:00:00Z
modified: 2015-05-08T01:10:55Z
discontinued: false
dominant: false
group u list: _267_
m user id: 0
owner id: 2512
owning group id: 58
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This is the GAL1 inducible promoter for use in Saccharomyces cerevisiae. It is repressed in the presence of glucose and induced in the presence of galactose. Raffinose is a neutral sugar source the neither induces nor represses the promoter. pGAL1 normally has an "all-or-nothing" response to galactose, but Christina Smolke has shown that knockout of the Gal2 gene gives pGAL1 a tunable, linear response to galactose.
mutable notes: none
mutable provenance: From the Saccharomyces cerevisiae genome, via Caroline Ajo-Franklin.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K165001_sequence/1

BBa_K1585110
type: ComponentDefinition
persistent identity: BBa K1585110
display id: BBa_K1585110
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Anderson Promoter with lacI binding site
creator: Tobias Schwanemann
created: 2015-08-31T11:00:00Z
modified: 2015-09-01T02:09:56Z
discontinued: false
dominant: false
experience: experience/Fails
group u list: _2002_
m user id: 24474
owner id: 24474
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Promoters of the Anderson Promoter Collection with lacI binding site derived from [http://parts.igem.org/Part:BBa_R0010 R_0010] to make them [http://openwetware.org/wiki/IPTG IPTG] iducible.
mutable notes: We choosed lacI-binding-site of part BBa_R0010 because we assumed older parts would be more reliable. The part is shipped in backbone [http://parts.igem.org/Part:BBa_J61002 J61002] because the functionality is directly visible by adding IPTG. RFP ([http://parts.igem.org/Part:BBa_E1010 E1010]) should get expressed then.
mutable provenance: Assambly of [http://parts.igem.org/Part:BBa_J23106 J23106] and lacI-binding-site of [http://parts.igem.org/Part:BBa_R0010 R_0010] The "lacI binding site" of Part [http://parts.igem.org/Part:BBa_R0010 R_0010] differs from lacO from Part [http://parts.igem.org/Part:BBa_J33207 BBa_J33207].
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
component: BBa_J23110
component: BBa_K1585999
sequence annotation: BBa_J23110
sequence annotation: BBa_K1585999
sequence: https://synbiohub.org/public/igem/BBa_K1585110_sequence/1

BBa_J23110
type: Component
persistent identity: component2441699
display id: component2441699
version: 1
top level: BBa_K1585110
access: public
definition: BBa_J23110

BBa_K1585999
type: Component
persistent identity: component2441701
display id: component2441701
version: 1
top level: BBa_K1585110
access: public
definition: https://synbiohub.org/public/igem/BBa_K1585999/1

BBa_J23110
type: SequenceAnnotation
persistent identity: annotation2441699
display id: annotation2441699
version: 1
top level: BBa_K1585110
location: https://synbiohub.org/public/igem/BBa_K1585110/annotation2441699/range2441699/1
component: BBa_J23110

https://synbiohub.org/public/igem/BBa_K1585110/annotation2441699/range2441699/1
type: Range
persistent identity: range2441699
display id: range2441699
version: 1
direction: direction/reverse
top level: BBa_K1585110
start: 1
end: 35
orientation: inline

BBa_K1585999
type: SequenceAnnotation
persistent identity: annotation2441701
display id: annotation2441701
version: 1
top level: BBa_K1585110
location: https://synbiohub.org/public/igem/BBa_K1585110/annotation2441701/range2441701/1
component: BBa_K1585999

https://synbiohub.org/public/igem/BBa_K1585110/annotation2441701/range2441701/1
type: Range
persistent identity: range2441701
display id: range2441701
version: 1
direction: direction/reverse
top level: BBa_K1585110
start: 44
end: 78
orientation: inline

LasR+PAI
type: ComponentDefinition
persistent identity: BBa R0079
display id: BBa_R0079
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter (LasR & PAI regulated)
creator: Alvin Carter Powers (Fighting Darwins)
created: 2004-01-27T12:00:00Z
modified: 2015-05-08T01:14:15Z
discontinued: false
dominant: true
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Binding region for LasR protein (positive regulation)
mutable provenance: "Analysis of the Pseudomonas aeruginosa Elastase (lasB) Regulatory Region". Lynn Rust, Everett Pesci, and Barbara Iglewski
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: OP2
sequence annotation: OP1
sequence annotation: -35
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R0079_sequence/1

OP2
type: SequenceAnnotation
persistent identity: annotation300985
display id: annotation300985
version: 1
top level: LasR+PAI
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0079/annotation300985/range300985/1

https://synbiohub.org/public/igem/BBa_R0079/annotation300985/range300985/1
type: Range
persistent identity: range300985
display id: range300985
version: 1
direction: direction/forward
top level: LasR+PAI
start: 46
end: 63
orientation: inline

OP1
type: SequenceAnnotation
persistent identity: annotation300992
display id: annotation300992
version: 1
top level: LasR+PAI
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0079/annotation300992/range300992/1

https://synbiohub.org/public/igem/BBa_R0079/annotation300992/range300992/1
type: Range
persistent identity: range300992
display id: range300992
version: 1
direction: direction/forward
top level: LasR+PAI
start: 106
end: 125
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation318495
display id: annotation318495
version: 1
top level: LasR+PAI
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0079/annotation318495/range318495/1

https://synbiohub.org/public/igem/BBa_R0079/annotation318495/range318495/1
type: Range
persistent identity: range318495
display id: range318495
version: 1
direction: direction/forward
top level: LasR+PAI
start: 117
end: 122
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation318496
display id: annotation318496
version: 1
top level: LasR+PAI
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0079/annotation318496/range318496/1

https://synbiohub.org/public/igem/BBa_R0079/annotation318496/range318496/1
type: Range
persistent identity: range318496
display id: range318496
version: 1
direction: direction/forward
top level: LasR+PAI
start: 140
end: 145
orientation: inline

const.
type: ComponentDefinition
persistent identity: BBa R1075
display id: BBa_R1075
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Constitutive Promoter II
creator: Chris Walsh (Fighting Darwins)
created: 2004-01-27T12:00:00Z
modified: 2015-05-08T01:14:15Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: constitutive promoter which outputs .4 TIPs point mutants of wild type rrnB promoter P1
mutable provenance: Gaal, Gourse, et al.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -10
sequence annotation: -35
sequence: https://synbiohub.org/public/igem/BBa_R1075_sequence/1

-10
type: SequenceAnnotation
persistent identity: annotation309020
display id: annotation309020
version: 1
top level: const.
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R1075/annotation309020/range309020/1

https://synbiohub.org/public/igem/BBa_R1075/annotation309020/range309020/1
type: Range
persistent identity: range309020
display id: range309020
version: 1
direction: direction/forward
top level: const.
start: 35
end: 40
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation309024
display id: annotation309024
version: 1
top level: const.
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R1075/annotation309024/range309024/1

https://synbiohub.org/public/igem/BBa_R1075/annotation309024/range309024/1
type: Range
persistent identity: range309024
display id: range309024
version: 1
direction: direction/forward
top level: const.
start: 15
end: 20
orientation: inline

T7 0.4
type: ComponentDefinition
persistent identity: BBa B2002
display id: BBa_B2002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: T7 RBS 0.4
creator: Heather Keller
created: 2006-09-27T11:00:00Z
modified: 2015-08-31T04:07:21Z
discontinued: false
dominant: true
experience: experience/None
group u list: _11_
m user id: 0
owner id: 571
owning group id: 10
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is the -20 to -1 region of T7 gp0.7, which contains the native RBS region for this gene. The physical instantiation of this part does not exist. This part is not compatible with Standard Biobricks assembly methods, as the scar created during assembly would interrupt the spacing between the RBS and the start codon of the CDS. Individuals wishing to use this RBS sequence in their assembles should refer to part XXXXX. (Information forthcoming)
mutable notes: The -20 to -1 region was chosen to be representative of the RBS region of the T7 genes for the following reasons: 1) The Shine-Delgarno region is contained within this defined region for all T7 genes. 2) Current literature suggests that when bound to the Translation Start Site (0), the Ribosome covers positions -20 to +13.
mutable provenance: Genome of wild-type bacteriophage T7, positions 2001-2020. Includes the -20 to -1 region of gp0.7.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: SD region
sequence: https://synbiohub.org/public/igem/BBa_B2002_sequence/1

SD region
type: SequenceAnnotation
persistent identity: annotation1902419
display id: annotation1902419
version: 1
top level: T7 0.4
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_B2002/annotation1902419/range1902419/1

https://synbiohub.org/public/igem/BBa_B2002/annotation1902419/range1902419/1
type: Range
persistent identity: range1902419
display id: range1902419
version: 1
direction: direction/forward
top level: T7 0.4
start: 4
end: 10
orientation: inline

lux pR
type: ComponentDefinition
persistent identity: BBa R0062
display id: BBa_R0062
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter (luxR & HSL regulated -- lux pR)
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:15Z
discontinued: false
dominant: true
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Promoter activated by LuxR in concert with HSL The lux cassette of V. fischeri contains a left and a right promoter. The right promoter gives weak constitutive expression of downstream genes.This expression is up-regulated by the action of the LuxR activator protein complexed with the autoinducer, 3-oxo-hexanoyl-HSL. Two molecules of LuxR protein form a complex with two molecules of the signalling compound homoserine lactone (HSL). This complex binds to a palindromic site on the promoter, increasing the rate of transcription.
mutable notes: This promoter is based on the Vibrio fischeri quorum sensing gene promoters. Two genes LuxI and LuxR and transcribed in opposite directions as shown below. The original sequence from which the parts <bb_part>BBa_R0062</bb_part> and <bb_part>BBa_R0063</bb_part> were derived is shown in the picture below. <img src="<bb_file>Image1.gif</bb_file>" width="614" height="362">
mutable provenance: V. fischeri
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: start
sequence annotation: -10
sequence annotation: -35
sequence annotation: BBa_R0062
sequence annotation: LuxR/HSL
sequence: https://synbiohub.org/public/igem/BBa_R0062_sequence/1

start
type: SequenceAnnotation
persistent identity: annotation2048
display id: annotation2048
version: 1
top level: lux pR
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_R0062/annotation2048/range2048/1

https://synbiohub.org/public/igem/BBa_R0062/annotation2048/range2048/1
type: Range
persistent identity: range2048
display id: range2048
version: 1
direction: direction/forward
top level: lux pR
start: 53
end: 53
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2047
display id: annotation2047
version: 1
top level: lux pR
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0062/annotation2047/range2047/1

https://synbiohub.org/public/igem/BBa_R0062/annotation2047/range2047/1
type: Range
persistent identity: range2047
display id: range2047
version: 1
direction: direction/forward
top level: lux pR
start: 42
end: 47
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2046
display id: annotation2046
version: 1
top level: lux pR
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0062/annotation2046/range2046/1

https://synbiohub.org/public/igem/BBa_R0062/annotation2046/range2046/1
type: Range
persistent identity: range2046
display id: range2046
version: 1
direction: direction/forward
top level: lux pR
start: 20
end: 25
orientation: inline

BBa_R0062
type: SequenceAnnotation
persistent identity: annotation7070
display id: annotation7070
version: 1
top level: lux pR
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_R0062/annotation7070/range7070/1

https://synbiohub.org/public/igem/BBa_R0062/annotation7070/range7070/1
type: Range
persistent identity: range7070
display id: range7070
version: 1
direction: direction/forward
top level: lux pR
start: 1
end: 55
orientation: inline

LuxR/HSL
type: SequenceAnnotation
persistent identity: annotation2045
display id: annotation2045
version: 1
top level: lux pR
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0062/annotation2045/range2045/1

https://synbiohub.org/public/igem/BBa_R0062/annotation2045/range2045/1
type: Range
persistent identity: range2045
display id: range2045
version: 1
direction: direction/forward
top level: lux pR
start: 1
end: 20
orientation: inline

Pnot(O21)
type: ComponentDefinition
persistent identity: BBa I732273
display id: BBa_I732273
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Family Member with Hybrid Operator (D001O21)
creator: Zhan Jian
created: 2007-10-13T11:00:00Z
modified: 2015-08-31T04:07:57Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732273_sequence/1

BBa_K259005
type: ComponentDefinition
persistent identity: BBa K259005
display id: BBa_K259005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: AraC Rheostat Promoter
creator: Petros Mina
created: 2009-09-02T11:00:00Z
modified: 2015-05-08T01:11:42Z
discontinued: false
dominant: false
group u list: _357_
m user id: 0
owner id: 5318
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: The AraC/pBAD promoter can be used with prokaryotic chassis to regulate expresion of proteins. When the AraC/pBAD promoter is placed upstream of your canditate translational unit (protein to be expressed) it allows control of the expression of the said protein. This is done via controlling the concentration of arabinose sugar in the environment/solution of bacterial growth. This composite part does not have an LVA degradation tag at its end, hence can be used with all prokaryotic assemblies.
mutable notes: The part was designed and assembled from the iGEM2009 kit with standard RFC10 assembly.
mutable provenance: The AraC promoter is found naturally in E.Coli at 1.52 min/centisome in the clockwise orientation. More information can be found at [http://ecogene.org/geneinfo.php?eg_id=EG10054 EcoGene].
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_R0080
component: BBa_R0081
sequence annotation: BBa_R0080
sequence annotation: BBa_R0081
sequence: https://synbiohub.org/public/igem/BBa_K259005_sequence/1

BBa_R0080
type: Component
persistent identity: component2018294
display id: component2018294
version: 1
top level: BBa_K259005
access: public
definition: https://synbiohub.org/public/igem/BBa_R0080/1

BBa_R0081
type: Component
persistent identity: component2018287
display id: component2018287
version: 1
top level: BBa_K259005
access: public
definition: https://synbiohub.org/public/igem/BBa_R0081/1

BBa_R0080
type: SequenceAnnotation
persistent identity: annotation2018294
display id: annotation2018294
version: 1
top level: BBa_K259005
location: https://synbiohub.org/public/igem/BBa_K259005/annotation2018294/range2018294/1
component: BBa_R0080

https://synbiohub.org/public/igem/BBa_K259005/annotation2018294/range2018294/1
type: Range
persistent identity: range2018294
display id: range2018294
version: 1
direction: direction/reverse
top level: BBa_K259005
start: 192
end: 340
orientation: inline

BBa_R0081
type: SequenceAnnotation
persistent identity: annotation2018287
display id: annotation2018287
version: 1
top level: BBa_K259005
location: https://synbiohub.org/public/igem/BBa_K259005/annotation2018287/range2018287/1
component: BBa_R0081

https://synbiohub.org/public/igem/BBa_K259005/annotation2018287/range2018287/1
type: Range
persistent identity: range2018287
display id: range2018287
version: 1
direction: direction/reverse
top level: BBa_K259005
start: 1
end: 183
orientation: inline

BBa_J64010
type: ComponentDefinition
persistent identity: BBa J64010
display id: BBa_J64010
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: lasI promoter
creator: Jeffrey J Tabor
created: 2007-01-15T12:00:00Z
modified: 2015-08-31T01:56:26Z
discontinued: false
dominant: false
experience: experience/None
group u list: _98_
m user id: 0
owner id: 88
owning group id: 98
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Promoter driving the lasI gene in Pseudomonas aeruginosa. Activated by LasR+3OC12HSL and not RhlR+C4HSL (Seed et al., 1995) ~4x stronger than the lasB promoter (BBa_R0079; Seed et al., 1995). Switch point ~0.1nM 3OC12HSL (Seed et al., 1995)
mutable notes: Promoter sequence cropped to 4nt upstream of Las box according to example of Whiteley and Greenberg, J. Bac, 2001
mutable provenance: Genomic sequence of Pseudomonas aeruginosa
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: +1
sequence annotation: Las box
sequence: https://synbiohub.org/public/igem/BBa_J64010_sequence/1

+1
type: SequenceAnnotation
persistent identity: annotation1910616
display id: annotation1910616
version: 1
top level: BBa_J64010
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_J64010/annotation1910616/range1910616/1

https://synbiohub.org/public/igem/BBa_J64010/annotation1910616/range1910616/1
type: Range
persistent identity: range1910616
display id: range1910616
version: 1
direction: direction/forward
top level: BBa_J64010
start: 53
end: 53
orientation: inline

Las box
type: SequenceAnnotation
persistent identity: annotation1910615
display id: annotation1910615
version: 1
top level: BBa_J64010
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_J64010/annotation1910615/range1910615/1

https://synbiohub.org/public/igem/BBa_J64010/annotation1910615/range1910615/1
type: Range
persistent identity: range1910615
display id: range1910615
version: 1
direction: direction/forward
top level: BBa_J64010
start: 5
end: 20
orientation: inline

RBS-GsiB
type: ComponentDefinition
persistent identity: BBa K143020
display id: BBa_K143020
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: GsiB ribosome binding site (RBS) for B. subtilis
creator: James Chappell
created: 2008-09-14T11:00:00Z
modified: 2015-05-08T01:10:23Z
discontinued: false
dominant: false
experience: experience/None
group u list: _199_
m user id: 0
owner id: 2090
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: GsiB is an endogenous ribosome binding site from ''B.subtilis''. The sequence of the gsiB ribosome binding site is '''AAAGGAGG''' which is complementary to the sequence '''UUUCCUCC''' from the 3' region of the 16s rRNA from ''B.subtilis''. GsiB is an endogenous ribosome binding site (RBS) from ''B.subtilis''. The sequence of the gsiB ribosome binding site is '''AAAGGAGG''' which is complementary to the sequence '''UUUCCUCC''' from the 3' region of the 16s rRNA from ''B.subtilis''. Previous research showed that the predicted binding energy of the 16s rRNA to the RBS is -9.3kcal.
mutable notes: In order to ensure that the RBS is functional the actual ribosome binding site was maintained and the distance between the RBS and the start codon maintained. In order to conform to the biobrick standard the sequence flanking the RBS had to be changed but the distance between the promoter and RBS, and start codon and RBS was maintained.
mutable provenance: The sequence was taken from a previous research paper [1] and was constructed by Geneart
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: rbs
sequence: https://synbiohub.org/public/igem/BBa_K143020_sequence/1

rbs
type: SequenceAnnotation
persistent identity: annotation1975872
display id: annotation1975872
version: 1
top level: RBS-GsiB
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K143020/annotation1975872/range1975872/1

https://synbiohub.org/public/igem/BBa_K143020/annotation1975872/range1975872/1
type: Range
persistent identity: range1975872
display id: range1975872
version: 1
direction: direction/forward
top level: RBS-GsiB
start: 2
end: 8
orientation: inline

BBa_K598020
type: ComponentDefinition
persistent identity: BBa K598020
display id: BBa_K598020
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: B0015+pBAD+vioAB+B0015+pBAD+TPP Up-regulated Hammerhead Ribozyme 1.20+vioD+B0015+pBAD+vioE
creator: Zhenrun ZHANG
created: 2011-09-27T11:00:00Z
modified: 2015-05-08T01:12:50Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _770_
m user id: 0
owner id: 8606
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: B0015+pBAD+vioAB+B0015+pBAD+TPP ribozyme 1.20+vioD+B0015+pBAD+vioE
mutable notes: The Ribosomal Binding Site of vioD is contained in TPP ribozyme 1.20.
mutable provenance: The hammerhead ribozyme comes from E. coli, and the TPP aptamer comes from B. subtilis.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
sequence annotation: B0015
sequence annotation: vioE
sequence annotation: rbs
sequence annotation: pBAD
sequence annotation: vioB
sequence annotation: TPP ribozyme 1.20
sequence annotation: vioA
sequence annotation: vioD
sequence: https://synbiohub.org/public/igem/BBa_K598020_sequence/1

B0015
type: SequenceAnnotation
persistent identity: annotation2154514
display id: annotation2154514
version: 1
top level: BBa_K598020
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K598020/annotation2154514/range2154514/1

https://synbiohub.org/public/igem/BBa_K598020/annotation2154514/range2154514/1
type: Range
persistent identity: range2154514
display id: range2154514
version: 1
direction: direction/forward
top level: BBa_K598020
start: 1
end: 129
orientation: inline

vioE
type: SequenceAnnotation
persistent identity: annotation2154586
display id: annotation2154586
version: 1
top level: BBa_K598020
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K598020/annotation2154586/range2154586/1

https://synbiohub.org/public/igem/BBa_K598020/annotation2154586/range2154586/1
type: Range
persistent identity: range2154586
display id: range2154586
version: 1
direction: direction/forward
top level: BBa_K598020
start: 6489
end: 7064
orientation: inline

B0015
type: SequenceAnnotation
persistent identity: annotation2154583
display id: annotation2154583
version: 1
top level: BBa_K598020
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K598020/annotation2154583/range2154583/1

https://synbiohub.org/public/igem/BBa_K598020/annotation2154583/range2154583/1
type: Range
persistent identity: range2154583
display id: range2154583
version: 1
direction: direction/forward
top level: BBa_K598020
start: 6205
end: 6333
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation2154575
display id: annotation2154575
version: 1
top level: BBa_K598020
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K598020/annotation2154575/range2154575/1

https://synbiohub.org/public/igem/BBa_K598020/annotation2154575/range2154575/1
type: Range
persistent identity: range2154575
display id: range2154575
version: 1
direction: direction/forward
top level: BBa_K598020
start: 4976
end: 4982
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation2154588
display id: annotation2154588
version: 1
top level: BBa_K598020
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K598020/annotation2154588/range2154588/1

https://synbiohub.org/public/igem/BBa_K598020/annotation2154588/range2154588/1
type: Range
persistent identity: range2154588
display id: range2154588
version: 1
direction: direction/forward
top level: BBa_K598020
start: 6477
end: 6483
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation2154518
display id: annotation2154518
version: 1
top level: BBa_K598020
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K598020/annotation2154518/range2154518/1

https://synbiohub.org/public/igem/BBa_K598020/annotation2154518/range2154518/1
type: Range
persistent identity: range2154518
display id: range2154518
version: 1
direction: direction/forward
top level: BBa_K598020
start: 1545
end: 1551
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation2154516
display id: annotation2154516
version: 1
top level: BBa_K598020
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K598020/annotation2154516/range2154516/1

https://synbiohub.org/public/igem/BBa_K598020/annotation2154516/range2154516/1
type: Range
persistent identity: range2154516
display id: range2154516
version: 1
direction: direction/forward
top level: BBa_K598020
start: 273
end: 279
orientation: inline

pBAD
type: SequenceAnnotation
persistent identity: annotation2154515
display id: annotation2154515
version: 1
top level: BBa_K598020
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K598020/annotation2154515/range2154515/1

https://synbiohub.org/public/igem/BBa_K598020/annotation2154515/range2154515/1
type: Range
persistent identity: range2154515
display id: range2154515
version: 1
direction: direction/forward
top level: BBa_K598020
start: 138
end: 267
orientation: inline

pBAD
type: SequenceAnnotation
persistent identity: annotation2154548
display id: annotation2154548
version: 1
top level: BBa_K598020
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K598020/annotation2154548/range2154548/1

https://synbiohub.org/public/igem/BBa_K598020/annotation2154548/range2154548/1
type: Range
persistent identity: range2154548
display id: range2154548
version: 1
direction: direction/forward
top level: BBa_K598020
start: 4702
end: 4831
orientation: inline

B0015
type: SequenceAnnotation
persistent identity: annotation2154545
display id: annotation2154545
version: 1
top level: BBa_K598020
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K598020/annotation2154545/range2154545/1

https://synbiohub.org/public/igem/BBa_K598020/annotation2154545/range2154545/1
type: Range
persistent identity: range2154545
display id: range2154545
version: 1
direction: direction/forward
top level: BBa_K598020
start: 4565
end: 4693
orientation: inline

pBAD
type: SequenceAnnotation
persistent identity: annotation2154577
display id: annotation2154577
version: 1
top level: BBa_K598020
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K598020/annotation2154577/range2154577/1

https://synbiohub.org/public/igem/BBa_K598020/annotation2154577/range2154577/1
type: Range
persistent identity: range2154577
display id: range2154577
version: 1
direction: direction/forward
top level: BBa_K598020
start: 6342
end: 6471
orientation: inline

vioB
type: SequenceAnnotation
persistent identity: annotation2154542
display id: annotation2154542
version: 1
top level: BBa_K598020
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K598020/annotation2154542/range2154542/1

https://synbiohub.org/public/igem/BBa_K598020/annotation2154542/range2154542/1
type: Range
persistent identity: range2154542
display id: range2154542
version: 1
direction: direction/forward
top level: BBa_K598020
start: 1559
end: 4556
orientation: inline

TPP ribozyme 1.20
type: SequenceAnnotation
persistent identity: annotation2154556
display id: annotation2154556
version: 1
top level: BBa_K598020
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K598020/annotation2154556/range2154556/1

https://synbiohub.org/public/igem/BBa_K598020/annotation2154556/range2154556/1
type: Range
persistent identity: range2154556
display id: range2154556
version: 1
direction: direction/forward
top level: BBa_K598020
start: 4838
end: 4987
orientation: inline

vioA
type: SequenceAnnotation
persistent identity: annotation2154517
display id: annotation2154517
version: 1
top level: BBa_K598020
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K598020/annotation2154517/range2154517/1

https://synbiohub.org/public/igem/BBa_K598020/annotation2154517/range2154517/1
type: Range
persistent identity: range2154517
display id: range2154517
version: 1
direction: direction/forward
top level: BBa_K598020
start: 285
end: 1542
orientation: inline

vioD
type: SequenceAnnotation
persistent identity: annotation2154576
display id: annotation2154576
version: 1
top level: BBa_K598020
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K598020/annotation2154576/range2154576/1

https://synbiohub.org/public/igem/BBa_K598020/annotation2154576/range2154576/1
type: Range
persistent identity: range2154576
display id: range2154576
version: 1
direction: direction/forward
top level: BBa_K598020
start: 4988
end: 6112
orientation: inline

BBa_K389051
type: ComponentDefinition
persistent identity: BBa K389051
display id: BBa_K389051
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Tightly regulated lacO + luc
creator: Jonas Aretz
created: 2010-10-07T11:00:00Z
modified: 2015-05-08T01:12:19Z
discontinued: false
dominant: false
experience: experience/Fails
group u list: _517_
m user id: 0
owner id: 6670
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is the tightly regulated lac operon K389050 with a luciferase gene as readout.
mutable notes: lacIq integrated in promoter so good regulation, luciferase as sensitive readout
mutable provenance: partsregistry, pGL4.10luc2
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K389004
component: BBa_K389050
component: BBa_B0034
sequence annotation: BBa_B0034
sequence annotation: BBa_K389050
sequence annotation: BBa_K389004
sequence: https://synbiohub.org/public/igem/BBa_K389051_sequence/1

BBa_K389004
type: Component
persistent identity: component2273073
display id: component2273073
version: 1
top level: BBa_K389051
access: public
definition: https://synbiohub.org/public/igem/BBa_K389004/1

BBa_K389050
type: Component
persistent identity: component2273068
display id: component2273068
version: 1
top level: BBa_K389051
access: public
definition: BBa_K389050

BBa_B0034
type: Component
persistent identity: component2273070
display id: component2273070
version: 1
top level: BBa_K389051
access: public
definition: BBa_B0034

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2273070
display id: annotation2273070
version: 1
top level: BBa_K389051
location: https://synbiohub.org/public/igem/BBa_K389051/annotation2273070/range2273070/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K389051/annotation2273070/range2273070/1
type: Range
persistent identity: range2273070
display id: range2273070
version: 1
direction: direction/reverse
top level: BBa_K389051
start: 1609
end: 1620
orientation: inline

BBa_K389050
type: SequenceAnnotation
persistent identity: annotation2273068
display id: annotation2273068
version: 1
top level: BBa_K389051
location: https://synbiohub.org/public/igem/BBa_K389051/annotation2273068/range2273068/1
component: BBa_K389050

https://synbiohub.org/public/igem/BBa_K389051/annotation2273068/range2273068/1
type: Range
persistent identity: range2273068
display id: range2273068
version: 1
direction: direction/reverse
top level: BBa_K389051
start: 1
end: 1600
orientation: inline

BBa_K389004
type: SequenceAnnotation
persistent identity: annotation2273073
display id: annotation2273073
version: 1
top level: BBa_K389051
location: https://synbiohub.org/public/igem/BBa_K389051/annotation2273073/range2273073/1
component: BBa_K389004

https://synbiohub.org/public/igem/BBa_K389051/annotation2273073/range2273073/1
type: Range
persistent identity: range2273073
display id: range2273073
version: 1
direction: direction/reverse
top level: BBa_K389051
start: 1627
end: 3282
orientation: inline

BBa_K586000
type: ComponentDefinition
persistent identity: BBa K586000
display id: BBa_K586000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Cup-1 Heavy metal sensor
creator: Chris Thompson
created: 2011-06-21T11:00:00Z
modified: 2015-05-08T01:12:47Z
discontinued: false
dominant: false
experience: experience/None
group u list: _757_
m user id: 0
owner id: 9336
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Cup-1 is a regulatory promoter that senses heavy metals such as copper, arsenic, cadmium, antimony, mercury, chromium, and selenium.
mutable notes: None
mutable provenance: Comes from genomic DNA of saccharomyces cerevisiae.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: Cup-1
sequence: https://synbiohub.org/public/igem/BBa_K586000_sequence/1

Cup-1
type: SequenceAnnotation
persistent identity: annotation2122521
display id: annotation2122521
version: 1
top level: BBa_K586000
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K586000/annotation2122521/range2122521/1

https://synbiohub.org/public/igem/BBa_K586000/annotation2122521/range2122521/1
type: Range
persistent identity: range2122521
display id: range2122521
version: 1
direction: direction/forward
top level: BBa_K586000
start: 1
end: 186
orientation: inline

BBa_M13508
type: ComponentDefinition
persistent identity: BBa M13508
display id: BBa_M13508
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: M13K07 gene VIII RBS
creator: Natalie Kuldell
created: 2006-12-14T12:00:00Z
modified: 2015-05-08T01:13:56Z
discontinued: false
dominant: true
experience: experience/None
group u list: _45_
m user id: 0
owner id: 314
owning group id: 1
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This RBS is part of the bacteriophage M13 genome, initiating translation of the gene VIII message (BBa_M13008). The part aligns with base pairs 1285-1300 in M13K07 genome. It was identified as the RBS for gene VIII message by Wezenbeek et al in Gene (1980) 11: 129-148
mutable notes: none
mutable provenance: New England Biolabs M13K07. Sequenced in 2002 by L. Panganaban
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_M13508_sequence/1

BBa_K259013
type: ComponentDefinition
persistent identity: BBa K259013
display id: BBa_K259013
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: AraC-RBS-GFP-Terminator
creator: Petros Mina
created: 2009-10-14T11:00:00Z
modified: 2015-05-08T01:11:42Z
discontinued: false
dominant: false
group u list: _357_
m user id: 0
owner id: 5318
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: A part used to quantify the AraC-RBS construct K259007 created by the BCCS-Bristol iGEM'09 team.
mutable notes: RFC10 assembly
mutable provenance: This part was designed with the specified biobricks from the parts registry. AraC occurs naturally in E.coli. GFP occurs in jellyfish species, RBS designed by the Anderson family and Terminator by Resma Shetty.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_R0081
component: BBa_R0080
component: BBa_B0012
component: BBa_E0040
component: BBa_B0011
component: BBa_J61100
sequence annotation: BBa_J61100
sequence annotation: BBa_B0012
sequence annotation: BBa_R0080
sequence annotation: BBa_R0081
sequence annotation: BBa_B0011
sequence annotation: BBa_E0040
sequence: https://synbiohub.org/public/igem/BBa_K259013_sequence/1

BBa_R0081
type: Component
persistent identity: component2041436
display id: component2041436
version: 1
top level: BBa_K259013
access: public
definition: https://synbiohub.org/public/igem/BBa_R0081/1

BBa_R0080
type: Component
persistent identity: component2041443
display id: component2041443
version: 1
top level: BBa_K259013
access: public
definition: https://synbiohub.org/public/igem/BBa_R0080/1

BBa_B0012
type: Component
persistent identity: component2041447
display id: component2041447
version: 1
top level: BBa_K259013
access: public
definition: BBa_B0012

BBa_E0040
type: Component
persistent identity: component2041446
display id: component2041446
version: 1
top level: BBa_K259013
access: public
definition: https://synbiohub.org/public/igem/BBa_E0040/1

BBa_B0011
type: Component
persistent identity: component2041451
display id: component2041451
version: 1
top level: BBa_K259013
access: public
definition: https://synbiohub.org/public/igem/BBa_B0011/1

BBa_J61100
type: Component
persistent identity: component2041444
display id: component2041444
version: 1
top level: BBa_K259013
access: public
definition: BBa_J61100

BBa_J61100
type: SequenceAnnotation
persistent identity: annotation2041444
display id: annotation2041444
version: 1
top level: BBa_K259013
location: https://synbiohub.org/public/igem/BBa_K259013/annotation2041444/range2041444/1
component: BBa_J61100

https://synbiohub.org/public/igem/BBa_K259013/annotation2041444/range2041444/1
type: Range
persistent identity: range2041444
display id: range2041444
version: 1
direction: direction/reverse
top level: BBa_K259013
start: 349
end: 360
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2041447
display id: annotation2041447
version: 1
top level: BBa_K259013
location: https://synbiohub.org/public/igem/BBa_K259013/annotation2041447/range2041447/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K259013/annotation2041447/range2041447/1
type: Range
persistent identity: range2041447
display id: range2041447
version: 1
direction: direction/forward
top level: BBa_K259013
start: 1095
end: 1135
orientation: inline

BBa_R0080
type: SequenceAnnotation
persistent identity: annotation2041443
display id: annotation2041443
version: 1
top level: BBa_K259013
location: https://synbiohub.org/public/igem/BBa_K259013/annotation2041443/range2041443/1
component: BBa_R0080

https://synbiohub.org/public/igem/BBa_K259013/annotation2041443/range2041443/1
type: Range
persistent identity: range2041443
display id: range2041443
version: 1
direction: direction/reverse
top level: BBa_K259013
start: 192
end: 340
orientation: inline

BBa_R0081
type: SequenceAnnotation
persistent identity: annotation2041436
display id: annotation2041436
version: 1
top level: BBa_K259013
location: https://synbiohub.org/public/igem/BBa_K259013/annotation2041436/range2041436/1
component: BBa_R0081

https://synbiohub.org/public/igem/BBa_K259013/annotation2041436/range2041436/1
type: Range
persistent identity: range2041436
display id: range2041436
version: 1
direction: direction/reverse
top level: BBa_K259013
start: 1
end: 183
orientation: inline

BBa_B0011
type: SequenceAnnotation
persistent identity: annotation2041451
display id: annotation2041451
version: 1
top level: BBa_K259013
location: https://synbiohub.org/public/igem/BBa_K259013/annotation2041451/range2041451/1
component: BBa_B0011

https://synbiohub.org/public/igem/BBa_K259013/annotation2041451/range2041451/1
type: Range
persistent identity: range2041451
display id: range2041451
version: 1
direction: direction/forward
top level: BBa_K259013
start: 1144
end: 1189
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation2041446
display id: annotation2041446
version: 1
top level: BBa_K259013
location: https://synbiohub.org/public/igem/BBa_K259013/annotation2041446/range2041446/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_K259013/annotation2041446/range2041446/1
type: Range
persistent identity: range2041446
display id: range2041446
version: 1
direction: direction/reverse
top level: BBa_K259013
start: 367
end: 1086
orientation: inline

BBa_K109200
type: ComponentDefinition
persistent identity: BBa K109200
display id: BBa_K109200
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: AraC and TetR promoter (hybrid)
creator: Faifan Tantakitti
created: 2008-07-27T11:00:00Z
modified: 2015-05-08T01:09:07Z
discontinued: false
dominant: false
experience: experience/None
group u list: _250_
m user id: 0
owner id: 3345
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This is lacI. trial version.
mutable notes: ......blank2....
mutable provenance: .....blank1...
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: TetR
sequence annotation: -35
sequence annotation: -10
sequence annotation: AraC
sequence: https://synbiohub.org/public/igem/BBa_K109200_sequence/1

TetR
type: SequenceAnnotation
persistent identity: annotation1969066
display id: annotation1969066
version: 1
top level: BBa_K109200
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K109200/annotation1969066/range1969066/1

https://synbiohub.org/public/igem/BBa_K109200/annotation1969066/range1969066/1
type: Range
persistent identity: range1969066
display id: range1969066
version: 1
direction: direction/forward
top level: BBa_K109200
start: 81
end: 99
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1980470
display id: annotation1980470
version: 1
top level: BBa_K109200
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K109200/annotation1980470/range1980470/1

https://synbiohub.org/public/igem/BBa_K109200/annotation1980470/range1980470/1
type: Range
persistent identity: range1980470
display id: range1980470
version: 1
direction: direction/forward
top level: BBa_K109200
start: 49
end: 54
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1980471
display id: annotation1980471
version: 1
top level: BBa_K109200
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K109200/annotation1980471/range1980471/1

https://synbiohub.org/public/igem/BBa_K109200/annotation1980471/range1980471/1
type: Range
persistent identity: range1980471
display id: range1980471
version: 1
direction: direction/forward
top level: BBa_K109200
start: 73
end: 78
orientation: inline

AraC
type: SequenceAnnotation
persistent identity: annotation1969061
display id: annotation1969061
version: 1
top level: BBa_K109200
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K109200/annotation1969061/range1969061/1

https://synbiohub.org/public/igem/BBa_K109200/annotation1969061/range1969061/1
type: Range
persistent identity: range1969061
display id: range1969061
version: 1
direction: direction/forward
top level: BBa_K109200
start: 34
end: 50
orientation: inline

BBa_B0053
type: ComponentDefinition
persistent identity: BBa B0053
display id: BBa_B0053
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Terminator (His)
creator: Caitlin Conboy
created: 2004-01-29T12:00:00Z
modified: 2015-08-31T04:07:20Z
discontinued: false
dominant: true
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Terminator from the E.coli His operon
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0053_sequence/1

stem_loop
type: SequenceAnnotation
persistent identity: annotation318602
display id: annotation318602
version: 1
top level: BBa_B0053
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_B0053/annotation318602/range318602/1

https://synbiohub.org/public/igem/BBa_B0053/annotation318602/range318602/1
type: Range
persistent identity: range318602
display id: range318602
version: 1
direction: direction/forward
top level: BBa_B0053
start: 9
end: 43
orientation: inline

U097O11+D0
type: ComponentDefinition
persistent identity: BBa I732414
display id: BBa_I732414
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Family Member (U097O11+D014O22)
creator: Zhan Jian
created: 2007-10-13T11:00:00Z
modified: 2015-08-31T04:07:58Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732414_sequence/1

BBa_K256028
type: ComponentDefinition
persistent identity: BBa K256028
display id: BBa_K256028
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: placI:CHE
creator: iGEM09_NTU-Singapore
created: 2009-10-18T11:00:00Z
modified: 2015-05-08T01:11:41Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _359_
m user id: 0
owner id: 4864
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: Generator of (CHE)Cholesterol esterase. Attached to placI, the part is inducible by IPTG and Lactose. It works better in Lactose.
mutable notes: --
mutable provenance: --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0010
component: BBa_K256023
component: BBa_B0012
component: BBa_R0010
component: BBa_B0034
sequence annotation: BBa_B0012
sequence annotation: BBa_K256023
sequence annotation: BBa_B0010
sequence annotation: BBa_R0010
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K256028_sequence/1

BBa_B0010
type: Component
persistent identity: component2052739
display id: component2052739
version: 1
top level: BBa_K256028
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_K256023
type: Component
persistent identity: component2052738
display id: component2052738
version: 1
top level: BBa_K256028
access: public
definition: https://synbiohub.org/public/igem/BBa_K256023/1

BBa_B0012
type: Component
persistent identity: component2052741
display id: component2052741
version: 1
top level: BBa_K256028
access: public
definition: BBa_B0012

BBa_R0010
type: Component
persistent identity: component2052729
display id: component2052729
version: 1
top level: BBa_K256028
access: public
definition: LacI

BBa_B0034
type: Component
persistent identity: component2052737
display id: component2052737
version: 1
top level: BBa_K256028
access: public
definition: BBa_B0034

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2052741
display id: annotation2052741
version: 1
top level: BBa_K256028
location: https://synbiohub.org/public/igem/BBa_K256028/annotation2052741/range2052741/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K256028/annotation2052741/range2052741/1
type: Range
persistent identity: range2052741
display id: range2052741
version: 1
direction: direction/forward
top level: BBa_K256028
start: 1265
end: 1305
orientation: inline

BBa_K256023
type: SequenceAnnotation
persistent identity: annotation2052738
display id: annotation2052738
version: 1
top level: BBa_K256028
location: https://synbiohub.org/public/igem/BBa_K256028/annotation2052738/range2052738/1
component: BBa_K256023

https://synbiohub.org/public/igem/BBa_K256028/annotation2052738/range2052738/1
type: Range
persistent identity: range2052738
display id: range2052738
version: 1
direction: direction/reverse
top level: BBa_K256028
start: 227
end: 1168
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2052739
display id: annotation2052739
version: 1
top level: BBa_K256028
location: https://synbiohub.org/public/igem/BBa_K256028/annotation2052739/range2052739/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K256028/annotation2052739/range2052739/1
type: Range
persistent identity: range2052739
display id: range2052739
version: 1
direction: direction/forward
top level: BBa_K256028
start: 1177
end: 1256
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation2052729
display id: annotation2052729
version: 1
top level: BBa_K256028
location: https://synbiohub.org/public/igem/BBa_K256028/annotation2052729/range2052729/1
component: BBa_R0010

https://synbiohub.org/public/igem/BBa_K256028/annotation2052729/range2052729/1
type: Range
persistent identity: range2052729
display id: range2052729
version: 1
direction: direction/forward
top level: BBa_K256028
start: 1
end: 200
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2052737
display id: annotation2052737
version: 1
top level: BBa_K256028
location: https://synbiohub.org/public/igem/BBa_K256028/annotation2052737/range2052737/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K256028/annotation2052737/range2052737/1
type: Range
persistent identity: range2052737
display id: range2052737
version: 1
direction: direction/reverse
top level: BBa_K256028
start: 209
end: 220
orientation: inline

BBa_K1709003
type: ComponentDefinition
persistent identity: BBa K1709003
display id: BBa_K1709003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CheZ-GFP
creator: Astrid Deryckere
created: 2015-09-15T11:00:00Z
modified: 2015-09-26T08:47:17Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2129_
m user id: 25389
owner id: 25389
owning group id: 9
status: status/Planning
bookmark: true
mutable description: Upon recognition of a small molecule (e.g. nutrients or toxins), chemoreceptors transduce a signal through a set of Che proteins. This group of methylesterases and phosphatases regulates the rotation of the flagella. At the end of the chain of Che proteins is CheY. In phosphorylated state, CheY binds the flagella and causes the cells to tumble. The phosphatase CheZ dephosphorylates CheY, inducing dissociation of CheY from the flagella enabling the cells to swim. Cells lacking the CheZ protein are unable to swim and will tumble excessively and incessantly.
mutable notes: 1 bp in BBa_K629003 was adapted to create an original restriciton site in the plasmid. CheZ was fused to GFP (BBa_K082003) and its LVA tag was codon-optimized to avoid introducing artificial DNA repetitions.
mutable provenance: DNA sequences originate from the E. Coli genome and previous biobrick sequences. The ribosome binding site was chosen from the Anderson RBS family.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_J61101
component: BBa_K1709002
sequence annotation: BBa_J61101
sequence annotation: BBa_K1709002
sequence: https://synbiohub.org/public/igem/BBa_K1709003_sequence/1

BBa_J61101
type: Component
persistent identity: component2472546
display id: component2472546
version: 1
top level: BBa_K1709003
access: public
definition: BBa_J61101

BBa_K1709002
type: Component
persistent identity: component2472552
display id: component2472552
version: 1
top level: BBa_K1709003
access: public
definition: CheZ-GFP

BBa_J61101
type: SequenceAnnotation
persistent identity: annotation2472546
display id: annotation2472546
version: 1
top level: BBa_K1709003
location: https://synbiohub.org/public/igem/BBa_K1709003/annotation2472546/range2472546/1
component: BBa_J61101

https://synbiohub.org/public/igem/BBa_K1709003/annotation2472546/range2472546/1
type: Range
persistent identity: range2472546
display id: range2472546
version: 1
direction: direction/reverse
top level: BBa_K1709003
start: 1
end: 12
orientation: inline

BBa_K1709002
type: SequenceAnnotation
persistent identity: annotation2472552
display id: annotation2472552
version: 1
top level: BBa_K1709003
location: https://synbiohub.org/public/igem/BBa_K1709003/annotation2472552/range2472552/1
component: BBa_K1709002

https://synbiohub.org/public/igem/BBa_K1709003/annotation2472552/range2472552/1
type: Range
persistent identity: range2472552
display id: range2472552
version: 1
direction: direction/reverse
top level: BBa_K1709003
start: 19
end: 1419
orientation: inline

BBa_I766556
type: ComponentDefinition
persistent identity: BBa I766556
display id: BBa_I766556
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pAdh (Strong) Promoter
creator: Nili Sommovilla
created: 2007-08-15T11:00:00Z
modified: 2015-08-31T04:08:10Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _155_
m user id: 0
owner id: 1931
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Strong Expression level Constitutive promoter in yeast
mutable notes: none
mutable provenance: Endogenous promoter for Alcohol Dehydrogenase
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I766556_sequence/1

OmpR
type: ComponentDefinition
persistent identity: BBa R0084
display id: BBa_R0084
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter (OmpR, positive)
creator: Stephen Lee, Roshan Kumar, Joe Levine, Ziyan Chu (Polkadorks, IAP 2004)
created: 2004-01-27T12:00:00Z
modified: 2015-05-08T01:14:15Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Positively regulated, OmpR-controlled promoter. This promoter is taken from the upstream region of ompF. Phosphorylated OmpR binds to the three operator sites and activates transcription.
mutable notes: The promoter includes three OmpR binding sites: F1, F2, and F3. The promoter starts at -107 and goes up to the transcriptional start site at +1. A distant fourth binding site, F4, has been deleted. It is involved in negative regulation of ompF (Head, Tardy and Kenney, 1998), so its deletion is intended to make the promoter solely activating. The efficacy of this promoter versus the ompC upstream region (BBa_R0082) or the truncated ompC upstream region (BBa_R0083) is currently unknown.
mutable provenance: NC_000913 E. coli K12
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -10
sequence annotation: F1 OmpR
sequence annotation: F2 OmpR
sequence annotation: F3 OmpR
sequence annotation: -35
sequence: https://synbiohub.org/public/igem/BBa_R0084_sequence/1

-10
type: SequenceAnnotation
persistent identity: annotation301318
display id: annotation301318
version: 1
top level: OmpR
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0084/annotation301318/range301318/1

https://synbiohub.org/public/igem/BBa_R0084/annotation301318/range301318/1
type: Range
persistent identity: range301318
display id: range301318
version: 1
direction: direction/forward
top level: OmpR
start: 88
end: 93
orientation: inline

F1 OmpR
type: SequenceAnnotation
persistent identity: annotation301308
display id: annotation301308
version: 1
top level: OmpR
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0084/annotation301308/range301308/1

https://synbiohub.org/public/igem/BBa_R0084/annotation301308/range301308/1
type: Range
persistent identity: range301308
display id: range301308
version: 1
direction: direction/forward
top level: OmpR
start: 11
end: 28
orientation: inline

F2 OmpR
type: SequenceAnnotation
persistent identity: annotation301309
display id: annotation301309
version: 1
top level: OmpR
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0084/annotation301309/range301309/1

https://synbiohub.org/public/igem/BBa_R0084/annotation301309/range301309/1
type: Range
persistent identity: range301309
display id: range301309
version: 1
direction: direction/forward
top level: OmpR
start: 31
end: 48
orientation: inline

F3 OmpR
type: SequenceAnnotation
persistent identity: annotation301313
display id: annotation301313
version: 1
top level: OmpR
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0084/annotation301313/range301313/1

https://synbiohub.org/public/igem/BBa_R0084/annotation301313/range301313/1
type: Range
persistent identity: range301313
display id: range301313
version: 1
direction: direction/forward
top level: OmpR
start: 51
end: 68
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation301317
display id: annotation301317
version: 1
top level: OmpR
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0084/annotation301317/range301317/1

https://synbiohub.org/public/igem/BBa_R0084/annotation301317/range301317/1
type: Range
persistent identity: range301317
display id: range301317
version: 1
direction: direction/forward
top level: OmpR
start: 73
end: 78
orientation: inline

BBa_K1486049
type: ComponentDefinition
persistent identity: BBa K1486049
display id: BBa_K1486049
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CpxR promoter FW + RFP
creator: EPFL iGem team 2014
created: 2014-10-06T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: to edit
mutable notes: to edit
mutable provenance: to edit
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_I13507
component: BBa_K1486046
sequence annotation: BBa_K1486046
sequence annotation: BBa_I13507
sequence: https://synbiohub.org/public/igem/BBa_K1486049_sequence/1

BBa_I13507
type: Component
persistent identity: component2398436
display id: component2398436
version: 1
top level: BBa_K1486049
access: public
definition: https://synbiohub.org/public/igem/BBa_I13507/1

BBa_K1486046
type: Component
persistent identity: component2398424
display id: component2398424
version: 1
top level: BBa_K1486049
access: public
definition: BBa_K1486046

BBa_K1486046
type: SequenceAnnotation
persistent identity: annotation2398424
display id: annotation2398424
version: 1
top level: BBa_K1486049
location: https://synbiohub.org/public/igem/BBa_K1486049/annotation2398424/range2398424/1
component: BBa_K1486046

https://synbiohub.org/public/igem/BBa_K1486049/annotation2398424/range2398424/1
type: Range
persistent identity: range2398424
display id: range2398424
version: 1
direction: direction/reverse
top level: BBa_K1486049
start: 1
end: 146
orientation: inline

BBa_I13507
type: SequenceAnnotation
persistent identity: annotation2398436
display id: annotation2398436
version: 1
top level: BBa_K1486049
location: https://synbiohub.org/public/igem/BBa_K1486049/annotation2398436/range2398436/1
component: BBa_I13507

https://synbiohub.org/public/igem/BBa_K1486049/annotation2398436/range2398436/1
type: Range
persistent identity: range2398436
display id: range2398436
version: 1
direction: direction/reverse
top level: BBa_K1486049
start: 155
end: 1015
orientation: inline

BBa_B0017
type: ComponentDefinition
persistent identity: BBa B0017
display id: BBa_B0017
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: double terminator (B0010-B0010)
creator: Austin Che
created: 2004-01-08T12:00:00Z
modified: 2015-08-31T04:07:20Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Double terminator with two copies of <bb_part>BBa_B0010</bb_part>
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000141
role: partType/Terminator
component: BBa_B0010
sequence annotation: BBa_B0010
sequence: https://synbiohub.org/public/igem/BBa_B0017_sequence/1

BBa_B0010
type: Component
persistent identity: component939331
display id: component939331
version: 1
top level: BBa_B0017
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0010
type: Component
persistent identity: component939337
display id: component939337
version: 1
top level: BBa_B0017
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation939331
display id: annotation939331
version: 1
top level: BBa_B0017
location: https://synbiohub.org/public/igem/BBa_B0017/annotation939331/range939331/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_B0017/annotation939331/range939331/1
type: Range
persistent identity: range939331
display id: range939331
version: 1
direction: direction/forward
top level: BBa_B0017
start: 1
end: 80
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation939337
display id: annotation939337
version: 1
top level: BBa_B0017
location: https://synbiohub.org/public/igem/BBa_B0017/annotation939337/range939337/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_B0017/annotation939337/range939337/1
type: Range
persistent identity: range939337
display id: range939337
version: 1
direction: direction/forward
top level: BBa_B0017
start: 89
end: 168
orientation: inline

BBa_K1486048
type: ComponentDefinition
persistent identity: BBa K1486048
display id: BBa_K1486048
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CpxR reporter
creator: EPFL iGem team 2014
created: 2014-10-06T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: Calgary's BBa_K339007 repaired (part didn't have the sequence they said they had.
mutable notes: to edit
mutable provenance: to edit
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_K135000
component: BBa_I13507
sequence annotation: BBa_K135000
sequence annotation: BBa_I13507
sequence: https://synbiohub.org/public/igem/BBa_K1486048_sequence/1

BBa_K135000
type: Component
persistent identity: component2398411
display id: component2398411
version: 1
top level: BBa_K1486048
access: public
definition: https://synbiohub.org/public/igem/BBa_K135000/1

BBa_I13507
type: Component
persistent identity: component2398423
display id: component2398423
version: 1
top level: BBa_K1486048
access: public
definition: https://synbiohub.org/public/igem/BBa_I13507/1

BBa_K135000
type: SequenceAnnotation
persistent identity: annotation2398411
display id: annotation2398411
version: 1
top level: BBa_K1486048
location: https://synbiohub.org/public/igem/BBa_K1486048/annotation2398411/range2398411/1
component: BBa_K135000

https://synbiohub.org/public/igem/BBa_K1486048/annotation2398411/range2398411/1
type: Range
persistent identity: range2398411
display id: range2398411
version: 1
direction: direction/reverse
top level: BBa_K1486048
start: 1
end: 55
orientation: inline

BBa_I13507
type: SequenceAnnotation
persistent identity: annotation2398423
display id: annotation2398423
version: 1
top level: BBa_K1486048
location: https://synbiohub.org/public/igem/BBa_K1486048/annotation2398423/range2398423/1
component: BBa_I13507

https://synbiohub.org/public/igem/BBa_K1486048/annotation2398423/range2398423/1
type: Range
persistent identity: range2398423
display id: range2398423
version: 1
direction: direction/reverse
top level: BBa_K1486048
start: 64
end: 924
orientation: inline

BBa_I732450
type: ComponentDefinition
persistent identity: BBa I732450
display id: BBa_I732450
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Family Member (U073O26+D062NUL)
creator: Zhan Jian
created: 2007-10-16T11:00:00Z
modified: 2015-08-31T04:07:58Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732450_sequence/1

pMalT
type: ComponentDefinition
persistent identity: BBa J03007
display id: BBa_J03007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Maltose specific promotor
creator: James Godman
created: 2005-11-03T12:00:00Z
modified: 2015-08-31T04:08:13Z
discontinued: false
dominant: false
experience: experience/Issues
group u list: _15_
m user id: 0
owner id: 405
owning group id: 15
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Binds Maltose and stimulates transcription
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J03007_sequence/1

BBa_J64804
type: ComponentDefinition
persistent identity: BBa J64804
display id: BBa_J64804
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: The promoter region (inclusive of regulator binding sites) of the B. subtilis RocDEF operon
creator: Sai Duriseti
created: 2007-03-19T12:00:00Z
modified: 2015-05-08T01:08:17Z
discontinued: false
dominant: false
group u list: _98_
m user id: 0
owner id: 1428
owning group id: 98
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is the reported promoter region from the Bacillus subtilis RocDEF operon as taken from the DBTBS website (http://dbtbs.hgc.jp/). Base 1 to base 21 contains the binding site for the binding factor, RocR. The sequence, TATGCAAAAGAATTTTGCACT, reportedly contains the consensus sequence for binding of this factor. Base 42 to base 62 contains another binding site for the RocR binding factor, with ATATCAGAATGTTTTTGCACC as the binding consensus for this sequence. Bases 113-135 outline the binding site of the binding factor AhrC (CTTGCATTTATATAAAGGGAAAG). Bases 96 to 135 outline the promoter region for this operon (CTTGATTTGGCACAGAACTTGCATTTATATAAAGGGAAAG).
mutable notes: This sequence only outlines the reported promoter region as ascertained from the online resource described above, and may lack nucleotides not described by the operon analysis used.
mutable provenance: Information was obtained from: http://dbtbs.hgc.jp/COG/prom/rocDEF.html.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: RocR Binding Site
sequence annotation: Promoter
sequence annotation: AhrC
sequence: https://synbiohub.org/public/igem/BBa_J64804_sequence/1

RocR Binding Site
type: SequenceAnnotation
persistent identity: annotation1920952
display id: annotation1920952
version: 1
top level: BBa_J64804
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_J64804/annotation1920952/range1920952/1

https://synbiohub.org/public/igem/BBa_J64804/annotation1920952/range1920952/1
type: Range
persistent identity: range1920952
display id: range1920952
version: 1
direction: direction/forward
top level: BBa_J64804
start: 42
end: 62
orientation: inline

Promoter
type: SequenceAnnotation
persistent identity: annotation1920953
display id: annotation1920953
version: 1
top level: BBa_J64804
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_J64804/annotation1920953/range1920953/1

https://synbiohub.org/public/igem/BBa_J64804/annotation1920953/range1920953/1
type: Range
persistent identity: range1920953
display id: range1920953
version: 1
direction: direction/forward
top level: BBa_J64804
start: 96
end: 135
orientation: inline

RocR Binding Site
type: SequenceAnnotation
persistent identity: annotation1920951
display id: annotation1920951
version: 1
top level: BBa_J64804
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_J64804/annotation1920951/range1920951/1

https://synbiohub.org/public/igem/BBa_J64804/annotation1920951/range1920951/1
type: Range
persistent identity: range1920951
display id: range1920951
version: 1
direction: direction/forward
top level: BBa_J64804
start: 1
end: 21
orientation: inline

AhrC
type: SequenceAnnotation
persistent identity: annotation1920955
display id: annotation1920955
version: 1
top level: BBa_J64804
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_J64804/annotation1920955/range1920955/1

https://synbiohub.org/public/igem/BBa_J64804/annotation1920955/range1920955/1
type: Range
persistent identity: range1920955
display id: range1920955
version: 1
direction: direction/forward
top level: BBa_J64804
start: 113
end: 131
orientation: inline

Zif23
type: ComponentDefinition
persistent identity: BBa R2002
display id: BBa_R2002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter, Zif23 regulated, test: between and after
creator: Ronny Krashinsky
created: 2004-01-29T12:00:00Z
modified: 2015-05-08T01:14:16Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Promoter with standard -10 and -35 sites. A symmetric Zif23 dimer target site is between the -10 and -35 sites, and a symmetric Zif23 dimer target site is after the -10 site.
mutable notes: consensus sequences based on [Jensen98].
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -35
sequence annotation: Zif23
sequence annotation: consensus
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R2002_sequence/1

-35
type: SequenceAnnotation
persistent identity: annotation318160
display id: annotation318160
version: 1
top level: Zif23
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R2002/annotation318160/range318160/1

https://synbiohub.org/public/igem/BBa_R2002/annotation318160/range318160/1
type: Range
persistent identity: range318160
display id: range318160
version: 1
direction: direction/forward
top level: Zif23
start: 10
end: 15
orientation: inline

Zif23
type: SequenceAnnotation
persistent identity: annotation318171
display id: annotation318171
version: 1
top level: Zif23
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R2002/annotation318171/range318171/1

https://synbiohub.org/public/igem/BBa_R2002/annotation318171/range318171/1
type: Range
persistent identity: range318171
display id: range318171
version: 1
direction: direction/forward
top level: Zif23
start: 20
end: 33
orientation: inline

Zif23
type: SequenceAnnotation
persistent identity: annotation318164
display id: annotation318164
version: 1
top level: Zif23
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R2002/annotation318164/range318164/1

https://synbiohub.org/public/igem/BBa_R2002/annotation318164/range318164/1
type: Range
persistent identity: range318164
display id: range318164
version: 1
direction: direction/forward
top level: Zif23
start: 39
end: 52
orientation: inline

consensus
type: SequenceAnnotation
persistent identity: annotation318174
display id: annotation318174
version: 1
top level: Zif23
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_R2002/annotation318174/range318174/1

https://synbiohub.org/public/igem/BBa_R2002/annotation318174/range318174/1
type: Range
persistent identity: range318174
display id: range318174
version: 1
direction: direction/forward
top level: Zif23
start: 1
end: 9
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation318163
display id: annotation318163
version: 1
top level: Zif23
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R2002/annotation318163/range318163/1

https://synbiohub.org/public/igem/BBa_R2002/annotation318163/range318163/1
type: Range
persistent identity: range318163
display id: range318163
version: 1
direction: direction/forward
top level: Zif23
start: 33
end: 38
orientation: inline

BBa_K165040
type: ComponentDefinition
persistent identity: BBa K165040
display id: BBa_K165040
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Gli1 binding sites + TEF constitutive yeast promoter
creator: John Szymanski
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:10:56Z
discontinued: false
dominant: false
group u list: _267_
m user id: 0
owner id: 2512
owning group id: 58
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: The Gli1 transcription factor binding sites on the TEF2 constitutive promoter. Transcription regulation should be constitutive plus regulated by Gli1-binding transcription factors.
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
component: BBa_K165037
component: BBa_K165012
sequence annotation: BBa_K165012
sequence annotation: BBa_K165037
sequence: https://synbiohub.org/public/igem/BBa_K165040_sequence/1

BBa_K165037
type: Component
persistent identity: component1996556
display id: component1996556
version: 1
top level: BBa_K165040
access: public
definition: pTEF

BBa_K165012
type: Component
persistent identity: component1996555
display id: component1996555
version: 1
top level: BBa_K165040
access: public
definition: https://synbiohub.org/public/igem/BBa_K165012/1

BBa_K165012
type: SequenceAnnotation
persistent identity: annotation1996555
display id: annotation1996555
version: 1
top level: BBa_K165040
location: https://synbiohub.org/public/igem/BBa_K165040/annotation1996555/range1996555/1
component: BBa_K165012

https://synbiohub.org/public/igem/BBa_K165040/annotation1996555/range1996555/1
type: Range
persistent identity: range1996555
display id: range1996555
version: 1
direction: direction/reverse
top level: BBa_K165040
start: 1
end: 127
orientation: inline

BBa_K165037
type: SequenceAnnotation
persistent identity: annotation1996556
display id: annotation1996556
version: 1
top level: BBa_K165040
location: https://synbiohub.org/public/igem/BBa_K165040/annotation1996556/range1996556/1
component: BBa_K165037

https://synbiohub.org/public/igem/BBa_K165040/annotation1996556/range1996556/1
type: Range
persistent identity: range1996556
display id: range1996556
version: 1
direction: direction/reverse
top level: BBa_K165040
start: 136
end: 538
orientation: inline

BBa_K136010
type: ComponentDefinition
persistent identity: BBa K136010
display id: BBa_K136010
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: fliA promoter
creator: Kok-Phen YAN
created: 2008-10-24T11:00:00Z
modified: 2015-05-08T01:10:02Z
discontinued: false
dominant: false
group u list: _211_
m user id: 0
owner id: 3262
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: FliA is a transcription factor involved in the formation of the flagella in E. coli. The promotor of fliA is induced by FlhDC. FliA seems to have a positive autoregulation on its promotor.
mutable notes: primers used
mutable provenance: This part comes from the genome of E. coli K12 strain MG1655.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: A mutated to G
sequence annotation: ATG
sequence annotation: transcription initiation site
sequence annotation: natural fliA RBS
sequence annotation: FlhDC binding site 1
sequence annotation: FlhDC binding site 2
sequence: https://synbiohub.org/public/igem/BBa_K136010_sequence/1

A mutated to G
type: SequenceAnnotation
persistent identity: annotation1987043
display id: annotation1987043
version: 1
top level: BBa_K136010
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K136010/annotation1987043/range1987043/1

https://synbiohub.org/public/igem/BBa_K136010/annotation1987043/range1987043/1
type: Range
persistent identity: range1987043
display id: range1987043
version: 1
direction: direction/forward
top level: BBa_K136010
start: 316
end: 316
orientation: inline

ATG
type: SequenceAnnotation
persistent identity: annotation1987044
display id: annotation1987044
version: 1
top level: BBa_K136010
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K136010/annotation1987044/range1987044/1

https://synbiohub.org/public/igem/BBa_K136010/annotation1987044/range1987044/1
type: Range
persistent identity: range1987044
display id: range1987044
version: 1
direction: direction/forward
top level: BBa_K136010
start: 342
end: 344
orientation: inline

transcription initiation site
type: SequenceAnnotation
persistent identity: annotation1987046
display id: annotation1987046
version: 1
top level: BBa_K136010
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K136010/annotation1987046/range1987046/1

https://synbiohub.org/public/igem/BBa_K136010/annotation1987046/range1987046/1
type: Range
persistent identity: range1987046
display id: range1987046
version: 1
direction: direction/forward
top level: BBa_K136010
start: 293
end: 293
orientation: inline

transcription initiation site
type: SequenceAnnotation
persistent identity: annotation1987045
display id: annotation1987045
version: 1
top level: BBa_K136010
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K136010/annotation1987045/range1987045/1

https://synbiohub.org/public/igem/BBa_K136010/annotation1987045/range1987045/1
type: Range
persistent identity: range1987045
display id: range1987045
version: 1
direction: direction/forward
top level: BBa_K136010
start: 282
end: 282
orientation: inline

natural fliA RBS
type: SequenceAnnotation
persistent identity: annotation1999348
display id: annotation1999348
version: 1
top level: BBa_K136010
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K136010/annotation1999348/range1999348/1

https://synbiohub.org/public/igem/BBa_K136010/annotation1999348/range1999348/1
type: Range
persistent identity: range1999348
display id: range1999348
version: 1
direction: direction/forward
top level: BBa_K136010
start: 330
end: 337
orientation: inline

FlhDC binding site 1
type: SequenceAnnotation
persistent identity: annotation1987040
display id: annotation1987040
version: 1
top level: BBa_K136010
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K136010/annotation1987040/range1987040/1

https://synbiohub.org/public/igem/BBa_K136010/annotation1987040/range1987040/1
type: Range
persistent identity: range1987040
display id: range1987040
version: 1
direction: direction/forward
top level: BBa_K136010
start: 198
end: 225
orientation: inline

FlhDC binding site 2
type: SequenceAnnotation
persistent identity: annotation1987041
display id: annotation1987041
version: 1
top level: BBa_K136010
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K136010/annotation1987041/range1987041/1

https://synbiohub.org/public/igem/BBa_K136010/annotation1987041/range1987041/1
type: Range
persistent identity: range1987041
display id: range1987041
version: 1
direction: direction/forward
top level: BBa_K136010
start: 226
end: 261
orientation: inline

A mutated to G
type: SequenceAnnotation
persistent identity: annotation1987042
display id: annotation1987042
version: 1
top level: BBa_K136010
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K136010/annotation1987042/range1987042/1

https://synbiohub.org/public/igem/BBa_K136010/annotation1987042/range1987042/1
type: Range
persistent identity: range1987042
display id: range1987042
version: 1
direction: direction/forward
top level: BBa_K136010
start: 263
end: 263
orientation: inline

BBa_K108025
type: ComponentDefinition
persistent identity: BBa K108025
display id: BBa_K108025
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Pu
creator: Qi Liu
created: 2008-10-22T11:00:00Z
modified: 2015-05-08T01:09:04Z
discontinued: false
dominant: false
experience: experience/None
group u list: _222_
m user id: 0
owner id: 2671
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: coming...
mutable notes: PCR using primers with biobrick prefix and surfix, and then cloned into pSB1AC3.
mutable provenance: pWW0
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: Pu
sequence: https://synbiohub.org/public/igem/BBa_K108025_sequence/1

Pu
type: SequenceAnnotation
persistent identity: annotation1986980
display id: annotation1986980
version: 1
top level: BBa_K108025
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K108025/annotation1986980/range1986980/1

https://synbiohub.org/public/igem/BBa_K108025/annotation1986980/range1986980/1
type: Range
persistent identity: range1986980
display id: range1986980
version: 1
direction: direction/forward
top level: BBa_K108025
start: 1
end: 200
orientation: inline

BBa_J56016
type: ComponentDefinition
persistent identity: BBa J56016
display id: BBa_J56016
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Rbs-orig - ribosome binding site
creator: Matt Eames
created: 2006-10-31T12:00:00Z
modified: 2015-08-31T03:22:59Z
discontinued: false
dominant: false
experience: experience/None
group u list: _79_98_
m user id: 0
owner id: 1132
owning group id: 98
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Ribosome binding site for transcription initiation
mutable notes: None
mutable provenance: Synthesized by DNA 2.0
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J56016_sequence/1

P3
type: ComponentDefinition
persistent identity: BBa K1781000
display id: BBa_K1781000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: P3 from M13
creator: Marvin Prein
created: 2015-09-06T11:00:00Z
modified: 2015-09-11T01:17:28Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2206_
m user id: 25533
owner id: 25533
owning group id: 9
sample status: No part sequence
status: status/Planning
bookmark: false
mutable description: will be edited
mutable notes: will be edited
mutable provenance: will be edited
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: P3
sequence: https://synbiohub.org/public/igem/BBa_K1781000_sequence/1

P3
type: SequenceAnnotation
persistent identity: annotation2448988
display id: annotation2448988
version: 1
top level: P3
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1781000/annotation2448988/range2448988/1

https://synbiohub.org/public/igem/BBa_K1781000/annotation2448988/range2448988/1
type: Range
persistent identity: range2448988
display id: range2448988
version: 1
direction: direction/forward
top level: P3
start: 1
end: 1302
orientation: inline

BBa_I766557
type: ComponentDefinition
persistent identity: BBa I766557
display id: BBa_I766557
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pSte5 (Weak) Promoter
creator: Nili Sommovilla
created: 2007-08-15T11:00:00Z
modified: 2015-08-31T04:08:10Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _155_
m user id: 0
owner id: 1931
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: low expression, constitutive promoter in yeast
mutable notes: none
mutable provenance: endogenous promoter for Sterile 5
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I766557_sequence/1

BBa_J61147
type: ComponentDefinition
persistent identity: BBa J61147
display id: BBa_J61147
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: {rbs1} Library Member in BBb
creator: John Anderson
created: 2007-09-03T11:00:00Z
modified: 2015-08-31T01:56:25Z
discontinued: false
dominant: false
experience: experience/None
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: {{BBbRBS1Family}}
mutable notes: {{BerkiGEM2007-Biobrick2.0Intro}}
mutable provenance: Synthetic
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J61147_sequence/1

BBa_J06403
type: ComponentDefinition
persistent identity: BBa J06403
display id: BBa_J06403
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RhIR promoter repressible by CI
creator: pbradley
created: 2005-06-23T11:00:00Z
modified: 2015-08-31T04:08:17Z
discontinued: false
dominant: false
experience: experience/None
group u list: _20_
m user id: 0
owner id: 358
owning group id: 20
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: THIS PART DOES NOT WORK. Need to add the CI operator sites.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: modified P(Rhl)
sequence annotation: RhlR
sequence annotation: -35
sequence annotation: BBa_I14017
sequence: https://synbiohub.org/public/igem/BBa_J06403_sequence/1

modified P(Rhl)
type: SequenceAnnotation
persistent identity: annotation1538365
display id: annotation1538365
version: 1
top level: BBa_J06403
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_J06403/annotation1538365/range1538365/1

https://synbiohub.org/public/igem/BBa_J06403/annotation1538365/range1538365/1
type: Range
persistent identity: range1538365
display id: range1538365
version: 1
direction: direction/forward
top level: BBa_J06403
start: 1
end: 51
orientation: inline

RhlR
type: SequenceAnnotation
persistent identity: annotation1538366
display id: annotation1538366
version: 1
top level: BBa_J06403
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_J06403/annotation1538366/range1538366/1

https://synbiohub.org/public/igem/BBa_J06403/annotation1538366/range1538366/1
type: Range
persistent identity: range1538366
display id: range1538366
version: 1
direction: direction/forward
top level: BBa_J06403
start: 1
end: 20
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1538367
display id: annotation1538367
version: 1
top level: BBa_J06403
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_J06403/annotation1538367/range1538367/1

https://synbiohub.org/public/igem/BBa_J06403/annotation1538367/range1538367/1
type: Range
persistent identity: range1538367
display id: range1538367
version: 1
direction: direction/forward
top level: BBa_J06403
start: 21
end: 50
orientation: inline

BBa_I14017
type: SequenceAnnotation
persistent identity: annotation1538368
display id: annotation1538368
version: 1
top level: BBa_J06403
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_J06403/annotation1538368/range1538368/1

https://synbiohub.org/public/igem/BBa_J06403/annotation1538368/range1538368/1
type: Range
persistent identity: range1538368
display id: range1538368
version: 1
direction: direction/forward
top level: BBa_J06403
start: 1
end: 51
orientation: inline

Bla
type: ComponentDefinition
persistent identity: BBa I14018
display id: BBa_I14018
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: P(Bla)
creator: Vikram Vijayan, Allen Hsu, Lawrence Fomundam
created: 2004-08-01T11:00:00Z
modified: 2015-08-31T04:07:37Z
discontinued: false
dominant: false
experience: experience/None
group u list: _4_
m user id: 0
owner id: 171
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: P(Bla), Medium Transcription
mutable provenance: Plasmid pUC19
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -10
sequence annotation: -35
sequence annotation: P(Bla)
sequence: https://synbiohub.org/public/igem/BBa_I14018_sequence/1

-10
type: SequenceAnnotation
persistent identity: annotation1027016
display id: annotation1027016
version: 1
top level: Bla
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I14018/annotation1027016/range1027016/1

https://synbiohub.org/public/igem/BBa_I14018/annotation1027016/range1027016/1
type: Range
persistent identity: range1027016
display id: range1027016
version: 1
direction: direction/forward
top level: Bla
start: 25
end: 30
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1027020
display id: annotation1027020
version: 1
top level: Bla
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I14018/annotation1027020/range1027020/1

https://synbiohub.org/public/igem/BBa_I14018/annotation1027020/range1027020/1
type: Range
persistent identity: range1027020
display id: range1027020
version: 1
direction: direction/forward
top level: Bla
start: 4
end: 9
orientation: inline

P(Bla)
type: SequenceAnnotation
persistent identity: annotation1018027
display id: annotation1018027
version: 1
top level: Bla
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_I14018/annotation1018027/range1018027/1

https://synbiohub.org/public/igem/BBa_I14018/annotation1018027/range1018027/1
type: Range
persistent identity: range1018027
display id: range1018027
version: 1
direction: direction/forward
top level: Bla
start: 1
end: 35
orientation: inline

BBa_K112148
type: ComponentDefinition
persistent identity: BBa K112148
display id: BBa_K112148
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: phoPp1 magnesium promoter
creator: Madhvi Venkatesh
created: 2008-11-01T12:00:00Z
modified: 2015-05-08T01:09:14Z
discontinued: false
dominant: false
group u list: _224_
m user id: 0
owner id: 3023
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: phoPp1 magnesium promoter
mutable notes: This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at: BBb Standard Description Page
mutable provenance: genome
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K112148_sequence/1

BBa_J61117
type: ComponentDefinition
persistent identity: BBa J61117
display id: BBa_J61117
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Ribosome Binding Site Family Member
creator: John Anderson
created: 2007-04-23T11:00:00Z
modified: 2015-08-31T01:59:49Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: false
mutable description: {{JCA_Arkin_RBSFamily}}
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J61117_sequence/1

BBa_K086033
type: ComponentDefinition
persistent identity: BBa K086033
display id: BBa_K086033
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: modified Lutz-Bujard LacO promoter,with alternative sigma factor σ38
creator: Gairik Sachdeva
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:08:37Z
discontinued: false
dominant: false
group u list: _197_
m user id: 0
owner id: 2578
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: modified Lutz-Bujard LacO promoter,with alternative sigma factor σ38
mutable notes: cleaned
mutable provenance: Lac O and sigma 38 binding sites conserved sequences
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: P38_4
sequence: https://synbiohub.org/public/igem/BBa_K086033_sequence/1

P38_4
type: SequenceAnnotation
persistent identity: annotation1995775
display id: annotation1995775
version: 1
top level: BBa_K086033
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K086033/annotation1995775/range1995775/1

https://synbiohub.org/public/igem/BBa_K086033/annotation1995775/range1995775/1
type: Range
persistent identity: range1995775
display id: range1995775
version: 1
direction: direction/forward
top level: BBa_K086033
start: 1
end: 55
orientation: inline

BBa_K1486024
type: ComponentDefinition
persistent identity: BBa K1486024
display id: BBa_K1486024
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Yeast kanamycin resistance
creator: EPFL iGem team 2014
created: 2014-09-25T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: To edit
mutable notes: To edit
mutable provenance: To edit
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: KanR
sequence annotation: TEF terminator
sequence annotation: TEF promoter
sequence: https://synbiohub.org/public/igem/BBa_K1486024_sequence/1

KanR
type: SequenceAnnotation
persistent identity: annotation2416943
display id: annotation2416943
version: 1
top level: BBa_K1486024
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1486024/annotation2416943/range2416943/1

https://synbiohub.org/public/igem/BBa_K1486024/annotation2416943/range2416943/1
type: Range
persistent identity: range2416943
display id: range2416943
version: 1
direction: direction/forward
top level: BBa_K1486024
start: 345
end: 1154
orientation: inline

TEF terminator
type: SequenceAnnotation
persistent identity: annotation2416944
display id: annotation2416944
version: 1
top level: BBa_K1486024
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1486024/annotation2416944/range2416944/1

https://synbiohub.org/public/igem/BBa_K1486024/annotation2416944/range2416944/1
type: Range
persistent identity: range2416944
display id: range2416944
version: 1
direction: direction/forward
top level: BBa_K1486024
start: 1159
end: 1357
orientation: inline

TEF promoter
type: SequenceAnnotation
persistent identity: annotation2416942
display id: annotation2416942
version: 1
top level: BBa_K1486024
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1486024/annotation2416942/range2416942/1

https://synbiohub.org/public/igem/BBa_K1486024/annotation2416942/range2416942/1
type: Range
persistent identity: range2416942
display id: range2416942
version: 1
direction: direction/forward
top level: BBa_K1486024
start: 1
end: 344
orientation: inline

U049O11+D0
type: ComponentDefinition
persistent identity: BBa I732441
display id: BBa_I732441
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Family Member (U049O11+D050O22)
creator: Zhan Jian
created: 2007-10-13T11:00:00Z
modified: 2015-08-31T04:07:58Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732441_sequence/1

U061O11+D0
type: ComponentDefinition
persistent identity: BBa I732431
display id: BBa_I732431
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Family Member (U061O11+D002O22)
creator: Zhan Jian
created: 2007-10-13T11:00:00Z
modified: 2015-08-31T04:07:58Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732431_sequence/1

pTEF
type: ComponentDefinition
persistent identity: BBa K124001
display id: BBa_K124001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Yeast TEF2 Constitutive Promoter
creator: John Szymanski
created: 2008-06-30T11:00:00Z
modified: 2015-05-08T01:09:44Z
discontinued: true
dominant: false
experience: experience/None
group u list: _267_
m user id: 0
owner id: 2512
owning group id: 58
part status: Released HQ 2013
sample status: Discontinued
status: status/Deleted
bookmark: false
mutable description: This is a mid-high level constitutive promoter from the yeast gene TEF2.
mutable notes: none
mutable provenance: Mumberg, Muller, and Funk. p413 pTEF http://dx.doi.org/10.1016/0378-1119(95)00037-7
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K124001_sequence/1

BBa_K180018
type: ComponentDefinition
persistent identity: BBa K180018
display id: BBa_K180018
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Scissors - Primary plasmid (part 1) [LasR generator]
creator: Richard Wilson, Stephanie Chambers
created: 2009-10-18T11:00:00Z
modified: 2015-05-08T01:11:06Z
discontinued: false
dominant: false
experience: experience/None
group u list: _278_
m user id: 0
owner id: 4082
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Part of the iGEM2009 Team:Southampton rock-paper-scissors 'scissors' primary plasmid.
mutable notes: Constitutive promoter selected to allow for continuous expression of C0179.
mutable provenance: See relevant basic biobrick parts (J23119, B0034, C0179, B0015)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_J23119
component: BBa_B0034
component: BBa_B0010
component: BBa_C0179
component: BBa_B0012
sequence annotation: BBa_C0179
sequence annotation: BBa_B0034
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_J23119
sequence: https://synbiohub.org/public/igem/BBa_K180018_sequence/1

BBa_J23119
type: Component
persistent identity: component2053912
display id: component2053912
version: 1
top level: BBa_K180018
access: public
definition: BBa_J23119

BBa_B0034
type: Component
persistent identity: component2053914
display id: component2053914
version: 1
top level: BBa_K180018
access: public
definition: BBa_B0034

BBa_B0010
type: Component
persistent identity: component2053916
display id: component2053916
version: 1
top level: BBa_K180018
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_C0179
type: Component
persistent identity: component2053915
display id: component2053915
version: 1
top level: BBa_K180018
access: public
definition: https://synbiohub.org/public/igem/BBa_C0179/1

BBa_B0012
type: Component
persistent identity: component2053918
display id: component2053918
version: 1
top level: BBa_K180018
access: public
definition: BBa_B0012

BBa_C0179
type: SequenceAnnotation
persistent identity: annotation2053915
display id: annotation2053915
version: 1
top level: BBa_K180018
location: https://synbiohub.org/public/igem/BBa_K180018/annotation2053915/range2053915/1
component: BBa_C0179

https://synbiohub.org/public/igem/BBa_K180018/annotation2053915/range2053915/1
type: Range
persistent identity: range2053915
display id: range2053915
version: 1
direction: direction/reverse
top level: BBa_K180018
start: 62
end: 784
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2053914
display id: annotation2053914
version: 1
top level: BBa_K180018
location: https://synbiohub.org/public/igem/BBa_K180018/annotation2053914/range2053914/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K180018/annotation2053914/range2053914/1
type: Range
persistent identity: range2053914
display id: range2053914
version: 1
direction: direction/reverse
top level: BBa_K180018
start: 44
end: 55
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2053916
display id: annotation2053916
version: 1
top level: BBa_K180018
location: https://synbiohub.org/public/igem/BBa_K180018/annotation2053916/range2053916/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K180018/annotation2053916/range2053916/1
type: Range
persistent identity: range2053916
display id: range2053916
version: 1
direction: direction/forward
top level: BBa_K180018
start: 793
end: 872
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2053918
display id: annotation2053918
version: 1
top level: BBa_K180018
location: https://synbiohub.org/public/igem/BBa_K180018/annotation2053918/range2053918/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K180018/annotation2053918/range2053918/1
type: Range
persistent identity: range2053918
display id: range2053918
version: 1
direction: direction/forward
top level: BBa_K180018
start: 881
end: 921
orientation: inline

BBa_J23119
type: SequenceAnnotation
persistent identity: annotation2053912
display id: annotation2053912
version: 1
top level: BBa_K180018
location: https://synbiohub.org/public/igem/BBa_K180018/annotation2053912/range2053912/1
component: BBa_J23119

https://synbiohub.org/public/igem/BBa_K180018/annotation2053912/range2053912/1
type: Range
persistent identity: range2053912
display id: range2053912
version: 1
direction: direction/reverse
top level: BBa_K180018
start: 1
end: 35
orientation: inline

BBa_I756020
type: ComponentDefinition
persistent identity: BBa I756020
display id: BBa_I756020
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Tet Operator in SV-40 intron
creator: Jack
created: 2007-08-15T11:00:00Z
modified: 2015-08-31T04:08:06Z
discontinued: false
dominant: false
group u list: _108_
m user id: 0
owner id: 2067
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Tet Operator in SV-40 intron
mutable notes: Tet Operator in SV-40 intron
mutable provenance: Tet Operator in SV-40 intron
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I756020_sequence/1

lacI+pL
type: ComponentDefinition
persistent identity: BBa R0011
display id: BBa_R0011
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter (lacI regulated, lambda pL hybrid)
creator: Neelaksh Varshney, Grace Kenney, Daniel Shen, Samantha Sutton
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) The PLlac 0-1 promoter is a hybrid regulatory region consisting of the promoter P(L) of phage lambda with the cI binding sites replaced with lacO1. The hybrid design allows for strong promotion that can nevertheless be tightly repressed by LacI, the Lac inhibitor (i.e. repressor) (<bb_part>BBa_C0010</bb_part>) ([LUTZ97]). The activity of the promoter can be regulated over a >600-fold range by IPTG in E.Coli DH5-alpha-Z1 (same paper reference).
mutable notes: hybrid promoter design to create strong promoter that is, at the same time, highly repressible. note that the upstream operator installed in this hybrid is slightly different than the one in the original source (Lutz and Bujard, 1997). the most upstream operator region is slightly truncated in the represillator version, so that both operators in the hybrid are the same sequence. see references for details. also, the sequence has been truncated after the transcriptional start site. LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and increase transcription. This part is incompatible with environments containing lactose or lactose analogs.
mutable provenance: represillator of Elowitz and Leibler (2000)
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: lac O1
sequence annotation: -35
sequence annotation: -10
sequence annotation: BBa_R0011
sequence: https://synbiohub.org/public/igem/BBa_R0011_sequence/1

lac O1
type: SequenceAnnotation
persistent identity: annotation1999
display id: annotation1999
version: 1
top level: lacI+pL
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_R0011/annotation1999/range1999/1

https://synbiohub.org/public/igem/BBa_R0011/annotation1999/range1999/1
type: Range
persistent identity: range1999
display id: range1999
version: 1
direction: direction/forward
top level: lacI+pL
start: 3
end: 19
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2000
display id: annotation2000
version: 1
top level: lacI+pL
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0011/annotation2000/range2000/1

https://synbiohub.org/public/igem/BBa_R0011/annotation2000/range2000/1
type: Range
persistent identity: range2000
display id: range2000
version: 1
direction: direction/forward
top level: lacI+pL
start: 20
end: 25
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2002
display id: annotation2002
version: 1
top level: lacI+pL
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0011/annotation2002/range2002/1

https://synbiohub.org/public/igem/BBa_R0011/annotation2002/range2002/1
type: Range
persistent identity: range2002
display id: range2002
version: 1
direction: direction/forward
top level: lacI+pL
start: 43
end: 48
orientation: inline

lac O1
type: SequenceAnnotation
persistent identity: annotation2001
display id: annotation2001
version: 1
top level: lacI+pL
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_R0011/annotation2001/range2001/1

https://synbiohub.org/public/igem/BBa_R0011/annotation2001/range2001/1
type: Range
persistent identity: range2001
display id: range2001
version: 1
direction: direction/forward
top level: lacI+pL
start: 26
end: 42
orientation: inline

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation7064
display id: annotation7064
version: 1
top level: lacI+pL
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_R0011/annotation7064/range7064/1

https://synbiohub.org/public/igem/BBa_R0011/annotation7064/range7064/1
type: Range
persistent identity: range7064
display id: range7064
version: 1
direction: direction/forward
top level: lacI+pL
start: 1
end: 54
orientation: inline

Kozak & st
type: ComponentDefinition
persistent identity: BBa J63003
display id: BBa_J63003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: designed yeast Kozak sequence
creator: Caroline Ajo-Franklin
created: 2006-10-10T11:00:00Z
modified: 2015-08-31T01:56:26Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _97_
m user id: 0
owner id: 545
owning group id: 97
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: consensus Kozak sequence and start codon
mutable notes: designed such that fusion using fusion bricks assembly method leads to in frame translation
mutable provenance: consensus Kozak and start built from oligonucleotides
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: start codon
sequence: https://synbiohub.org/public/igem/BBa_J63003_sequence/1

start codon
type: SequenceAnnotation
persistent identity: annotation1902852
display id: annotation1902852
version: 1
top level: Kozak & st
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_J63003/annotation1902852/range1902852/1

https://synbiohub.org/public/igem/BBa_J63003/annotation1902852/range1902852/1
type: Range
persistent identity: range1902852
display id: range1902852
version: 1
direction: direction/forward
top level: Kozak & st
start: 13
end: 15
orientation: inline

BBa_K678022
type: ComponentDefinition
persistent identity: BBa K678022
display id: BBa_K678022
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Neomycin cassette
creator: DTU-Denmark-2
created: 2011-09-03T11:00:00Z
modified: 2015-05-08T01:13:04Z
discontinued: false
dominant: false
experience: experience/None
group u list: _882_
m user id: 0
owner id: 8341
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Resistance cassette for selection of mammalian cells. The cassette contains a SV40 promoter, the neomycin resistance gene, and the SV40 poly A as a terminator.
mutable notes: Compatible with the Plug'n'Play assembly standard
mutable provenance: From plasmid pcDNA3.1 from Invitrogen
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Other
role: SO:0000110
sequence annotation: Neomycin cassette
sequence annotation: Linker 5
sequence: https://synbiohub.org/public/igem/BBa_K678022_sequence/1

Neomycin cassette
type: SequenceAnnotation
persistent identity: annotation2127359
display id: annotation2127359
version: 1
top level: BBa_K678022
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K678022/annotation2127359/range2127359/1

https://synbiohub.org/public/igem/BBa_K678022/annotation2127359/range2127359/1
type: Range
persistent identity: range2127359
display id: range2127359
version: 1
direction: direction/forward
top level: BBa_K678022
start: 9
end: 1512
orientation: inline

Linker 5
type: SequenceAnnotation
persistent identity: annotation2127358
display id: annotation2127358
version: 1
top level: BBa_K678022
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K678022/annotation2127358/range2127358/1

https://synbiohub.org/public/igem/BBa_K678022/annotation2127358/range2127358/1
type: Range
persistent identity: range2127358
display id: range2127358
version: 1
direction: direction/forward
top level: BBa_K678022
start: 1
end: 8
orientation: inline

BBa_B0033
type: ComponentDefinition
persistent identity: BBa B0033
display id: BBa_B0033
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS.4 (weaker) -- derivative of BBa_0030
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _41_44_48_46_1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Weaker RBS based on Ron Weiss thesis. Strengths relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>.
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("RBS-3" in figure 4-14 of thesis). No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: RBS-4\Weaker
sequence annotation: BBa_B0033
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_B0033_sequence/1

RBS-4\Weaker
type: SequenceAnnotation
persistent identity: annotation1713
display id: annotation1713
version: 1
top level: BBa_B0033
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_B0033/annotation1713/range1713/1

https://synbiohub.org/public/igem/BBa_B0033/annotation1713/range1713/1
type: Range
persistent identity: range1713
display id: range1713
version: 1
direction: direction/forward
top level: BBa_B0033
start: 1
end: 11
orientation: inline

BBa_B0033
type: SequenceAnnotation
persistent identity: annotation7028
display id: annotation7028
version: 1
top level: BBa_B0033
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_B0033/annotation7028/range7028/1

https://synbiohub.org/public/igem/BBa_B0033/annotation7028/range7028/1
type: Range
persistent identity: range7028
display id: range7028
version: 1
direction: direction/forward
top level: BBa_B0033
start: 1
end: 11
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation1714
display id: annotation1714
version: 1
top level: BBa_B0033
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_B0033/annotation1714/range1714/1

https://synbiohub.org/public/igem/BBa_B0033/annotation1714/range1714/1
type: Range
persistent identity: range1714
display id: range1714
version: 1
direction: direction/forward
top level: BBa_B0033
start: 7
end: 10
orientation: inline

BBa_K086021
type: ComponentDefinition
persistent identity: BBa K086021
display id: BBa_K086021
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: modified Lutz-Bujard LacO promoter,with alternative sigma factor σ24
creator: Gairik Sachdeva
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:08:36Z
discontinued: false
dominant: false
group u list: _197_
m user id: 0
owner id: 2578
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: modified Lutz-Bujard LacO promoter,with alternative sigma factor σ24
mutable notes: Cleaned
mutable provenance: Lac O and sigma 24 binding conserved sequences
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: P24_4
sequence: https://synbiohub.org/public/igem/BBa_K086021_sequence/1

P24_4
type: SequenceAnnotation
persistent identity: annotation1995755
display id: annotation1995755
version: 1
top level: BBa_K086021
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K086021/annotation1995755/range1995755/1

https://synbiohub.org/public/igem/BBa_K086021/annotation1995755/range1995755/1
type: Range
persistent identity: range1995755
display id: range1995755
version: 1
direction: direction/forward
top level: BBa_K086021
start: 1
end: 55
orientation: inline

BBa_J61116
type: ComponentDefinition
persistent identity: BBa J61116
display id: BBa_J61116
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Ribosome Binding Site Family Member
creator: John Anderson
created: 2007-04-23T11:00:00Z
modified: 2015-08-31T02:03:00Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: false
mutable description: {{JCA_Arkin_RBSFamily}}
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J61116_sequence/1

BBa_J61048
type: ComponentDefinition
persistent identity: BBa J61048
display id: BBa_J61048
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: [rnpB-T1] Terminator
creator: John Anderson
created: 2007-02-20T12:00:00Z
modified: 2015-08-31T02:03:00Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: false
mutable description: bob
mutable notes: bob
mutable provenance: bob
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence: https://synbiohub.org/public/igem/BBa_J61048_sequence/1

BBa_I12040
type: ComponentDefinition
persistent identity: BBa I12040
display id: BBa_I12040
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Modified lambda P(RM) promoter: -10 region from P(L) and cooperatively repressed by 434 cI
creator: ryhsiao
created: 2004-08-18T11:00:00Z
modified: 2015-08-31T04:07:31Z
discontinued: false
dominant: false
experience: experience/None
group u list: _3_
m user id: 0
owner id: 148
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Lamdba P(RM) promoter modified to be activated by lamda repressor (cI) and repressed by 434 repressor (cI). Two operator sites are used for both activation and repression to enhance cooperativity. Additionally, the -10 region from lambda P(L) was used instead of that from P(RM).
mutable notes: The O-R3 site of lambda (17 nt) was replaced by the O-R1 site of 434 (14 nt) to facilitate repression by 434 cI. The new 434 O-R1 is 5'-justified with the lambda O-R3 it replaced, leaving the -10 sequence intact. The 434 O-R2 site replaces the wildtype labmda promoter starting at -5, thereby preserving the 8 nt between 434 O-R1 and O-R2 in the wildtype. The mRNA transcript will contain an additional 9 nt compared to wildtype lambda phage. This part differs from BBa_I12036 because the -10 region from P(L) was used instead of that from P(RM).
mutable provenance: Bushman, F. D. The bacteriophage 434 right operator roles of O-R1, O-R2, and O-R3. J. Mol. Biol. (1993) 230, 28-40. ; Lutz, R & Bujard, H. "Independent and tight regulation of transcriptional units in Escherichia coli via the lacR/O, the TetR/O and AraC/I1-I2 regulatory elements, Nucleic Acids Research, 1997, Vol.25, No.6 (1203-1210)
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -35
sequence annotation: OR1 lambda
sequence annotation: OR2 lambda
sequence annotation: OR2 434
sequence annotation: -10
sequence annotation: OR1 434
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_I12040_sequence/1

-35
type: SequenceAnnotation
persistent identity: annotation1059300
display id: annotation1059300
version: 1
top level: BBa_I12040
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I12040/annotation1059300/range1059300/1

https://synbiohub.org/public/igem/BBa_I12040/annotation1059300/range1059300/1
type: Range
persistent identity: range1059300
display id: range1059300
version: 1
direction: direction/forward
top level: BBa_I12040
start: 48
end: 53
orientation: inline

OR1 lambda
type: SequenceAnnotation
persistent identity: annotation1059298
display id: annotation1059298
version: 1
top level: BBa_I12040
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I12040/annotation1059298/range1059298/1

https://synbiohub.org/public/igem/BBa_I12040/annotation1059298/range1059298/1
type: Range
persistent identity: range1059298
display id: range1059298
version: 1
direction: direction/forward
top level: BBa_I12040
start: 9
end: 25
orientation: inline

OR2 lambda
type: SequenceAnnotation
persistent identity: annotation1059299
display id: annotation1059299
version: 1
top level: BBa_I12040
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I12040/annotation1059299/range1059299/1

https://synbiohub.org/public/igem/BBa_I12040/annotation1059299/range1059299/1
type: Range
persistent identity: range1059299
display id: range1059299
version: 1
direction: direction/forward
top level: BBa_I12040
start: 33
end: 49
orientation: inline

OR2 434
type: SequenceAnnotation
persistent identity: annotation1059303
display id: annotation1059303
version: 1
top level: BBa_I12040
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I12040/annotation1059303/range1059303/1

https://synbiohub.org/public/igem/BBa_I12040/annotation1059303/range1059303/1
type: Range
persistent identity: range1059303
display id: range1059303
version: 1
direction: direction/forward
top level: BBa_I12040
start: 78
end: 91
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1059302
display id: annotation1059302
version: 1
top level: BBa_I12040
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I12040/annotation1059302/range1059302/1

https://synbiohub.org/public/igem/BBa_I12040/annotation1059302/range1059302/1
type: Range
persistent identity: range1059302
display id: range1059302
version: 1
direction: direction/forward
top level: BBa_I12040
start: 71
end: 76
orientation: inline

OR1 434
type: SequenceAnnotation
persistent identity: annotation1059301
display id: annotation1059301
version: 1
top level: BBa_I12040
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I12040/annotation1059301/range1059301/1

https://synbiohub.org/public/igem/BBa_I12040/annotation1059301/range1059301/1
type: Range
persistent identity: range1059301
display id: range1059301
version: 1
direction: direction/forward
top level: BBa_I12040
start: 56
end: 69
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation1062263
display id: annotation1062263
version: 1
top level: BBa_I12040
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_I12040/annotation1062263/range1062263/1

https://synbiohub.org/public/igem/BBa_I12040/annotation1062263/range1062263/1
type: Range
persistent identity: range1062263
display id: range1062263
version: 1
direction: direction/forward
top level: BBa_I12040
start: 83
end: 83
orientation: inline

BBa_M13108
type: ComponentDefinition
persistent identity: BBa M13108
display id: BBa_M13108
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: M13K07 gene VIII promoter
creator: Natalie Kuldell
created: 2006-12-15T12:00:00Z
modified: 2015-05-08T01:13:55Z
discontinued: false
dominant: true
group u list: _45_
m user id: 0
owner id: 314
owning group id: 1
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This part is from the bacteriophage M13 genome, bp 1155-1201 in M13K07. It directs transcription of M13 gene VIII (BBa_M13508, BBa_M13008). Its identity as a promoter was described in Gene (1980) 11:129-148 based on -10,-35 homology and fragment of genome able to bind RNAP.
mutable notes: none
mutable provenance: New England Biolabs M13K07. Sequenced in 2002 by L. Panganaban.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_M13108_sequence/1

BBa_K091110
type: ComponentDefinition
persistent identity: BBa K091110
display id: BBa_K091110
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LacI Promoter
creator: Robert Cool
created: 2008-06-19T11:00:00Z
modified: 2015-05-08T01:08:37Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _191_
m user id: 0
owner id: 3080
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This is a modified version of the LacI promoter that should bind LacI tighter than the original.
mutable notes: no special considerations
mutable provenance: This promoter is a modified version of the LacI promoter
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -10
sequence annotation: -35
sequence: https://synbiohub.org/public/igem/BBa_K091110_sequence/1

-10
type: SequenceAnnotation
persistent identity: annotation1964082
display id: annotation1964082
version: 1
top level: BBa_K091110
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K091110/annotation1964082/range1964082/1

https://synbiohub.org/public/igem/BBa_K091110/annotation1964082/range1964082/1
type: Range
persistent identity: range1964082
display id: range1964082
version: 1
direction: direction/forward
top level: BBa_K091110
start: 44
end: 49
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1964081
display id: annotation1964081
version: 1
top level: BBa_K091110
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K091110/annotation1964081/range1964081/1

https://synbiohub.org/public/igem/BBa_K091110/annotation1964081/range1964081/1
type: Range
persistent identity: range1964081
display id: range1964081
version: 1
direction: direction/forward
top level: BBa_K091110
start: 17
end: 22
orientation: inline

BBa_K1486008
type: ComponentDefinition
persistent identity: BBa K1486008
display id: BBa_K1486008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CxpR & Split IFP1.4 [Cterm + Cterm][1]
creator: iGem EPFL 2014
created: 2014-08-25T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part has two fused proteins downstream of an arabinose-inducible promoter: CpxR-IFP[1] and CpxR-IFP[2]. This construct was made in all orientations: - IFP[1]-CpxR + IFP[2]-CpxR (BBa_K1286009) - IFP[1]-CpxR + CpxR-IFP[2] (BBa_K1486010) - CpxR-IFP[1] + IFP[2]-CpxR (BBa_K1486011)
mutable notes: All orientations were made, so all dimerizations could be tested (see main page for biobrick numbers). Flexible linkers were used for each of the fused proteins, and an RBS was put before each one of them.
mutable provenance: From Michnick Article. To Edit
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0034
component: BBa_K1486000
component: BBa_K1486004
component: BBa_K1486052
component: BBa_K1486007
component: BBa_K1486006
component: BBa_I0500
sequence annotation: BBa_K1486052
sequence annotation: BBa_K1486007
sequence annotation: BBa_K1486000
sequence annotation: BBa_K1486004
sequence annotation: BBa_B0034
sequence annotation: BBa_I0500
sequence annotation: BBa_K1486006
sequence: https://synbiohub.org/public/igem/BBa_K1486008_sequence/1

BBa_B0034
type: Component
persistent identity: component2418048
display id: component2418048
version: 1
top level: BBa_K1486008
access: public
definition: BBa_B0034

BBa_K1486000
type: Component
persistent identity: component2418052
display id: component2418052
version: 1
top level: BBa_K1486008
access: public
definition: BBa_K1486000

BBa_K1486004
type: Component
persistent identity: component2418053
display id: component2418053
version: 1
top level: BBa_K1486008
access: public
definition: BBa_K1486004

BBa_K1486052
type: Component
persistent identity: component2418046
display id: component2418046
version: 1
top level: BBa_K1486008
access: public
definition: BBa_K1486052

BBa_B0034
type: Component
persistent identity: component2418036
display id: component2418036
version: 1
top level: BBa_K1486008
access: public
definition: BBa_B0034

BBa_K1486007
type: Component
persistent identity: component2418057
display id: component2418057
version: 1
top level: BBa_K1486008
access: public
definition: BBa_K1486007

BBa_K1486004
type: Component
persistent identity: component2418041
display id: component2418041
version: 1
top level: BBa_K1486008
access: public
definition: BBa_K1486004

BBa_K1486000
type: Component
persistent identity: component2418040
display id: component2418040
version: 1
top level: BBa_K1486008
access: public
definition: BBa_K1486000

BBa_K1486006
type: Component
persistent identity: component2418045
display id: component2418045
version: 1
top level: BBa_K1486008
access: public
definition: BBa_K1486006

BBa_I0500
type: Component
persistent identity: component2418034
display id: component2418034
version: 1
top level: BBa_K1486008
access: public
definition: pBad/araC

BBa_K1486052
type: SequenceAnnotation
persistent identity: annotation2418046
display id: annotation2418046
version: 1
top level: BBa_K1486008
location: https://synbiohub.org/public/igem/BBa_K1486008/annotation2418046/range2418046/1
component: BBa_K1486052

https://synbiohub.org/public/igem/BBa_K1486008/annotation2418046/range2418046/1
type: Range
persistent identity: range2418046
display id: range2418046
version: 1
direction: direction/reverse
top level: BBa_K1486008
start: 2360
end: 2397
orientation: inline

BBa_K1486007
type: SequenceAnnotation
persistent identity: annotation2418057
display id: annotation2418057
version: 1
top level: BBa_K1486008
location: https://synbiohub.org/public/igem/BBa_K1486008/annotation2418057/range2418057/1
component: BBa_K1486007

https://synbiohub.org/public/igem/BBa_K1486008/annotation2418057/range2418057/1
type: Range
persistent identity: range2418057
display id: range2418057
version: 1
direction: direction/reverse
top level: BBa_K1486008
start: 3142
end: 3726
orientation: inline

BBa_K1486000
type: SequenceAnnotation
persistent identity: annotation2418052
display id: annotation2418052
version: 1
top level: BBa_K1486008
location: https://synbiohub.org/public/igem/BBa_K1486008/annotation2418052/range2418052/1
component: BBa_K1486000

https://synbiohub.org/public/igem/BBa_K1486008/annotation2418052/range2418052/1
type: Range
persistent identity: range2418052
display id: range2418052
version: 1
direction: direction/reverse
top level: BBa_K1486008
start: 2410
end: 3111
orientation: inline

BBa_K1486004
type: SequenceAnnotation
persistent identity: annotation2418053
display id: annotation2418053
version: 1
top level: BBa_K1486008
location: https://synbiohub.org/public/igem/BBa_K1486008/annotation2418053/range2418053/1
component: BBa_K1486004

https://synbiohub.org/public/igem/BBa_K1486008/annotation2418053/range2418053/1
type: Range
persistent identity: range2418053
display id: range2418053
version: 1
direction: direction/reverse
top level: BBa_K1486008
start: 3112
end: 3141
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2418036
display id: annotation2418036
version: 1
top level: BBa_K1486008
location: https://synbiohub.org/public/igem/BBa_K1486008/annotation2418036/range2418036/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1486008/annotation2418036/range2418036/1
type: Range
persistent identity: range2418036
display id: range2418036
version: 1
direction: direction/reverse
top level: BBa_K1486008
start: 1211
end: 1222
orientation: inline

BBa_K1486004
type: SequenceAnnotation
persistent identity: annotation2418041
display id: annotation2418041
version: 1
top level: BBa_K1486008
location: https://synbiohub.org/public/igem/BBa_K1486008/annotation2418041/range2418041/1
component: BBa_K1486004

https://synbiohub.org/public/igem/BBa_K1486008/annotation2418041/range2418041/1
type: Range
persistent identity: range2418041
display id: range2418041
version: 1
direction: direction/reverse
top level: BBa_K1486008
start: 1925
end: 1954
orientation: inline

BBa_I0500
type: SequenceAnnotation
persistent identity: annotation2418034
display id: annotation2418034
version: 1
top level: BBa_K1486008
location: https://synbiohub.org/public/igem/BBa_K1486008/annotation2418034/range2418034/1
component: BBa_I0500

https://synbiohub.org/public/igem/BBa_K1486008/annotation2418034/range2418034/1
type: Range
persistent identity: range2418034
display id: range2418034
version: 1
direction: direction/reverse
top level: BBa_K1486008
start: 1
end: 1210
orientation: inline

BBa_K1486000
type: SequenceAnnotation
persistent identity: annotation2418040
display id: annotation2418040
version: 1
top level: BBa_K1486008
location: https://synbiohub.org/public/igem/BBa_K1486008/annotation2418040/range2418040/1
component: BBa_K1486000

https://synbiohub.org/public/igem/BBa_K1486008/annotation2418040/range2418040/1
type: Range
persistent identity: range2418040
display id: range2418040
version: 1
direction: direction/reverse
top level: BBa_K1486008
start: 1223
end: 1924
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2418048
display id: annotation2418048
version: 1
top level: BBa_K1486008
location: https://synbiohub.org/public/igem/BBa_K1486008/annotation2418048/range2418048/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1486008/annotation2418048/range2418048/1
type: Range
persistent identity: range2418048
display id: range2418048
version: 1
direction: direction/reverse
top level: BBa_K1486008
start: 2398
end: 2409
orientation: inline

BBa_K1486006
type: SequenceAnnotation
persistent identity: annotation2418045
display id: annotation2418045
version: 1
top level: BBa_K1486008
location: https://synbiohub.org/public/igem/BBa_K1486008/annotation2418045/range2418045/1
component: BBa_K1486006

https://synbiohub.org/public/igem/BBa_K1486008/annotation2418045/range2418045/1
type: Range
persistent identity: range2418045
display id: range2418045
version: 1
direction: direction/reverse
top level: BBa_K1486008
start: 1955
end: 2359
orientation: inline

BBa_J64030
type: ComponentDefinition
persistent identity: BBa J64030
display id: BBa_J64030
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: sopD RBS
creator: Dan Widmaier
created: 2008-01-16T12:00:00Z
modified: 2015-08-31T01:56:26Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _98_
m user id: 0
owner id: 2407
owning group id: 98
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is the natural RBS from the sopD gene in Salmonella Type III Secretion.
mutable notes: This part was designed without consideration for biobrick standard assembly. This RBS has not been explicitly tested for expression rate.
mutable provenance: Salmonella LT2 Genomic DNA
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: sopD RBS
sequence: https://synbiohub.org/public/igem/BBa_J64030_sequence/1

sopD RBS
type: SequenceAnnotation
persistent identity: annotation1959634
display id: annotation1959634
version: 1
top level: BBa_J64030
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_J64030/annotation1959634/range1959634/1

https://synbiohub.org/public/igem/BBa_J64030/annotation1959634/range1959634/1
type: Range
persistent identity: range1959634
display id: range1959634
version: 1
direction: direction/forward
top level: BBa_J64030
start: 1
end: 17
orientation: inline

BBa_K137088
type: ComponentDefinition
persistent identity: BBa K137088
display id: BBa_K137088
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: optimized (TA) repeat constitutive promoter with 19 bp between -10 and -35 elements
creator: Allen Lin
created: 2008-08-20T11:00:00Z
modified: 2015-05-08T01:10:10Z
discontinued: false
dominant: false
experience: experience/None
group u list: _187_
m user id: 0
owner id: 3112
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: optimized (TA) repeat constitutive promoter with 19 bp between -10 and -35 elements
mutable notes: None
mutable provenance: PCR
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K137088_sequence/1

BBa_J61100
type: ComponentDefinition
persistent identity: BBa J61100
display id: BBa_J61100
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Ribosome Binding Site Family Member
creator: John Anderson
created: 2007-01-28T12:00:00Z
modified: 2015-08-31T02:03:00Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: false
mutable description: {{JCA_Arkin_RBSFamily}}
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J61100_sequence/1

BBa_K1330002
type: ComponentDefinition
persistent identity: BBa K1330002
display id: BBa_K1330002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Constitutive promoter (J23105)
creator: Kristian Jensen
created: 2014-09-29T11:00:00Z
modified: 2015-05-08T01:09:55Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1705_
m user id: 0
owner id: 20126
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: t
mutable notes: t
mutable provenance: t
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K1330002_sequence/1

BBa_K091117
type: ComponentDefinition
persistent identity: BBa K091117
display id: BBa_K091117
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pLas promoter
creator: Erin Feeney
created: 2008-06-25T11:00:00Z
modified: 2015-05-08T01:08:37Z
discontinued: false
dominant: true
experience: experience/None
group u list: _191_
m user id: 0
owner id: 3067
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This pLas' promoter contains the regulatory region upstream of the LasI gene in Pseudomonas aeruginosa. K091117 includes a LasR binding site as well as a -10 region modified from CATAAA to TATAAA to reflect our pLasXOR promoter design. Activation is expected to occur in the presence of the autoinducer PAI-1 and the LasR protein. The pLas' promoter serves as a control for the evaluation of the pLasXOR promoter, part BBa_K091118.
mutable notes: To minimize leaky transcription, a secondary transcriptional start site was excluded from the design.
mutable provenance: The sequence of the LasI upstream regulatory region is available at http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=176640
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -10 Region
sequence annotation: Primary Transcriptional Start
sequence annotation: -35 Region
sequence annotation: LasR Binding Site
sequence: https://synbiohub.org/public/igem/BBa_K091117_sequence/1

-10 Region
type: SequenceAnnotation
persistent identity: annotation1964074
display id: annotation1964074
version: 1
top level: BBa_K091117
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K091117/annotation1964074/range1964074/1

https://synbiohub.org/public/igem/BBa_K091117/annotation1964074/range1964074/1
type: Range
persistent identity: range1964074
display id: range1964074
version: 1
direction: direction/forward
top level: BBa_K091117
start: 113
end: 118
orientation: inline

Primary Transcriptional Start
type: SequenceAnnotation
persistent identity: annotation1964075
display id: annotation1964075
version: 1
top level: BBa_K091117
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K091117/annotation1964075/range1964075/1

https://synbiohub.org/public/igem/BBa_K091117/annotation1964075/range1964075/1
type: Range
persistent identity: range1964075
display id: range1964075
version: 1
direction: direction/reverse
top level: BBa_K091117
start: 125
end: 125
orientation: inline

-35 Region
type: SequenceAnnotation
persistent identity: annotation1964072
display id: annotation1964072
version: 1
top level: BBa_K091117
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K091117/annotation1964072/range1964072/1

https://synbiohub.org/public/igem/BBa_K091117/annotation1964072/range1964072/1
type: Range
persistent identity: range1964072
display id: range1964072
version: 1
direction: direction/forward
top level: BBa_K091117
start: 86
end: 91
orientation: inline

LasR Binding Site
type: SequenceAnnotation
persistent identity: annotation1964073
display id: annotation1964073
version: 1
top level: BBa_K091117
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K091117/annotation1964073/range1964073/1

https://synbiohub.org/public/igem/BBa_K091117/annotation1964073/range1964073/1
type: Range
persistent identity: range1964073
display id: range1964073
version: 1
direction: direction/forward
top level: BBa_K091117
start: 75
end: 93
orientation: inline

BBa_K165034
type: ComponentDefinition
persistent identity: BBa K165034
display id: BBa_K165034
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Zif268-HIV bs + LexA bs + mCYC promoter
creator: John Szymanski
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:10:56Z
discontinued: false
dominant: false
group u list: _267_
m user id: 0
owner id: 2512
owning group id: 58
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: The Zif268-HIV and LexA transcription factor binding sites on the mCYC minimal promoter (in that order.) Transcription regulation comes from Zif268-HIV and LexA-binding transcription factors.
mutable notes: -
mutable provenance: Constructed in our lab with parts from Caroline Ajo-Franklin and David Drubin.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
component: BBa_K165011
component: BBa_K165031
sequence annotation: BBa_K165011
sequence annotation: BBa_K165031
sequence: https://synbiohub.org/public/igem/BBa_K165034_sequence/1

BBa_K165011
type: Component
persistent identity: component1995464
display id: component1995464
version: 1
top level: BBa_K165034
access: public
definition: https://synbiohub.org/public/igem/BBa_K165011/1

BBa_K165031
type: Component
persistent identity: component1995465
display id: component1995465
version: 1
top level: BBa_K165034
access: public
definition: BBa_K165031

BBa_K165011
type: SequenceAnnotation
persistent identity: annotation1995464
display id: annotation1995464
version: 1
top level: BBa_K165034
location: https://synbiohub.org/public/igem/BBa_K165034/annotation1995464/range1995464/1
component: BBa_K165011

https://synbiohub.org/public/igem/BBa_K165034/annotation1995464/range1995464/1
type: Range
persistent identity: range1995464
display id: range1995464
version: 1
direction: direction/reverse
top level: BBa_K165034
start: 1
end: 46
orientation: inline

BBa_K165031
type: SequenceAnnotation
persistent identity: annotation1995465
display id: annotation1995465
version: 1
top level: BBa_K165034
location: https://synbiohub.org/public/igem/BBa_K165034/annotation1995465/range1995465/1
component: BBa_K165031

https://synbiohub.org/public/igem/BBa_K165034/annotation1995465/range1995465/1
type: Range
persistent identity: range1995465
display id: range1995465
version: 1
direction: direction/reverse
top level: BBa_K165034
start: 55
end: 457
orientation: inline

BBa_K136003
type: ComponentDefinition
persistent identity: BBa K136003
display id: BBa_K136003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter of flhB gene from E. coli flagella
creator: Cyprien Maisonnier
created: 2008-08-11T11:00:00Z
modified: 2015-05-08T01:10:02Z
discontinued: true
dominant: false
experience: experience/None
group u list: _211_
m user id: 0
owner id: 3012
owning group id: 9
sample status: Discontinued
status: status/Deleted
bookmark: false
mutable description: ''flhB'' is a class 2 gene that is part of the construction of the flagella ( ''Ordering Genes in a Flagella Pathway by Analysis of Expression Kinetics from Living Bacteria'' - S. Kalir, J. McClure, K. Pabbaraju, C. Southward, M. Ronen, S. Leibler, M. G. Surette, U. Alon ). Its promoter is consitutively repressed. It is activated by FlhD4C2 and maybe by FliA with different strength ( ''Using a Quantitative Blueprint to Reprogram the Dynamics of the Flagella Gene Network'' - Shiraz Kalir and Uri Alon ). '''Work in progress :''' Deeper quantitative characterization
mutable notes: The primers used to amplify this sequence are : *Forward : GTTTCTTCGAATTCGCGGCCGCTTCTAGAGCCACGTCATATCAGGCGGTCTGATAAGG *Reverse : GTTTCTTCCTGCAGCGGCCGCTACTAGTAGTTTTGTCGTCGCTCTCGTCAGACACGTC
mutable provenance: This part comes from the genome of ''E.coli'' K12 strain MG1655.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: Initiation for Transcription
sequence annotation: FlhDC Binding site 2
sequence annotation: FlhDC Binding site 1
sequence: https://synbiohub.org/public/igem/BBa_K136003_sequence/1

Initiation for Transcription
type: SequenceAnnotation
persistent identity: annotation1971576
display id: annotation1971576
version: 1
top level: BBa_K136003
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K136003/annotation1971576/range1971576/1

https://synbiohub.org/public/igem/BBa_K136003/annotation1971576/range1971576/1
type: Range
persistent identity: range1971576
display id: range1971576
version: 1
direction: direction/forward
top level: BBa_K136003
start: 153
end: 153
orientation: inline

FlhDC Binding site 2
type: SequenceAnnotation
persistent identity: annotation1971575
display id: annotation1971575
version: 1
top level: BBa_K136003
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K136003/annotation1971575/range1971575/1

https://synbiohub.org/public/igem/BBa_K136003/annotation1971575/range1971575/1
type: Range
persistent identity: range1971575
display id: range1971575
version: 1
direction: direction/forward
top level: BBa_K136003
start: 106
end: 121
orientation: inline

FlhDC Binding site 1
type: SequenceAnnotation
persistent identity: annotation1971574
display id: annotation1971574
version: 1
top level: BBa_K136003
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K136003/annotation1971574/range1971574/1

https://synbiohub.org/public/igem/BBa_K136003/annotation1971574/range1971574/1
type: Range
persistent identity: range1971574
display id: range1971574
version: 1
direction: direction/forward
top level: BBa_K136003
start: 79
end: 94
orientation: inline

BBa_J61134
type: ComponentDefinition
persistent identity: BBa J61134
display id: BBa_J61134
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Ribosome Binding Site Family Member
creator: John Anderson
created: 2007-04-24T11:00:00Z
modified: 2015-08-31T01:59:49Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: false
mutable description: {{JCA_Arkin_RBSFamily}}
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J61134_sequence/1

BBa_K180003
type: ComponentDefinition
persistent identity: BBa K180003
display id: BBa_K180003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PTAC testing plasmid (GFP) - basic
creator: Richard Wilson, Stephanie Chambers
created: 2009-10-15T11:00:00Z
modified: 2015-05-08T01:11:06Z
discontinued: false
dominant: false
experience: experience/None
group u list: _278_
m user id: 0
owner id: 4082
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: As per BBa_K180002, omitting the RBS and STOP biobrick parts (B0034 and B0015) for speed and ease of construction.
mutable notes: Assess if the RBS (B0034) sequence is needed prior to GFP (E0040) whilst still able to test function of part K180000.
mutable provenance: See parts K180000, E0040
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_E0040
component: BBa_K180000
sequence annotation: BBa_K180000
sequence annotation: BBa_E0040
sequence: https://synbiohub.org/public/igem/BBa_K180003_sequence/1

BBa_E0040
type: Component
persistent identity: component2044074
display id: component2044074
version: 1
top level: BBa_K180003
access: public
definition: https://synbiohub.org/public/igem/BBa_E0040/1

BBa_K180000
type: Component
persistent identity: component2044072
display id: component2044072
version: 1
top level: BBa_K180003
access: public
definition: BBa_K180000

BBa_K180000
type: SequenceAnnotation
persistent identity: annotation2044072
display id: annotation2044072
version: 1
top level: BBa_K180003
location: https://synbiohub.org/public/igem/BBa_K180003/annotation2044072/range2044072/1
component: BBa_K180000

https://synbiohub.org/public/igem/BBa_K180003/annotation2044072/range2044072/1
type: Range
persistent identity: range2044072
display id: range2044072
version: 1
direction: direction/reverse
top level: BBa_K180003
start: 1
end: 1447
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation2044074
display id: annotation2044074
version: 1
top level: BBa_K180003
location: https://synbiohub.org/public/igem/BBa_K180003/annotation2044074/range2044074/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_K180003/annotation2044074/range2044074/1
type: Range
persistent identity: range2044074
display id: range2044074
version: 1
direction: direction/reverse
top level: BBa_K180003
start: 1454
end: 2173
orientation: inline

yA
type: ComponentDefinition
persistent identity: BBa K678035
display id: BBa_K678035
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: yA (green pigment) from Aspergillus nidulans
creator: DTU-Denmark-2
created: 2011-09-03T11:00:00Z
modified: 2015-05-08T01:13:04Z
discontinued: false
dominant: false
experience: experience/None
group u list: _882_
m user id: 0
owner id: 8341
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: The gene yA from Aspergillus nidulans encodes a p-diphenol oxidase (laccase) that converts a yellow pigment precursor to the mature green form. The green pigment is located in the walls of the conidia and protects the spores from damage by ultraviolet light. A yA mutant produces yellow spores.
mutable notes: Compatible with the Plug'n'Play assembly standard
mutable provenance: Aspergillus nidulans genomic DNA
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Linker 2
sequence annotation: yA
sequence: https://synbiohub.org/public/igem/BBa_K678035_sequence/1

Linker 2
type: SequenceAnnotation
persistent identity: annotation2127401
display id: annotation2127401
version: 1
top level: yA
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K678035/annotation2127401/range2127401/1

https://synbiohub.org/public/igem/BBa_K678035/annotation2127401/range2127401/1
type: Range
persistent identity: range2127401
display id: range2127401
version: 1
direction: direction/forward
top level: yA
start: 1
end: 8
orientation: inline

yA
type: SequenceAnnotation
persistent identity: annotation2127402
display id: annotation2127402
version: 1
top level: yA
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K678035/annotation2127402/range2127402/1

https://synbiohub.org/public/igem/BBa_K678035/annotation2127402/range2127402/1
type: Range
persistent identity: range2127402
display id: range2127402
version: 1
direction: direction/forward
top level: yA
start: 9
end: 2156
orientation: inline

BBa_I739107
type: ComponentDefinition
persistent identity: BBa I739107
display id: BBa_I739107
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Double Promoter (cI, negative / LacI, negative)
creator: Stefan Luzi
created: 2007-10-16T11:00:00Z
modified: 2015-08-31T04:08:01Z
discontinued: false
dominant: false
group u list: _124_
m user id: 0
owner id: 2183
owning group id: 62
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: asdf
mutable notes: sadf
mutable provenance: Synthetic DNA
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: LacI binding site
sequence annotation: R0010
sequence annotation: R0051
sequence: https://synbiohub.org/public/igem/BBa_I739107_sequence/1

LacI binding site
type: SequenceAnnotation
persistent identity: annotation1948511
display id: annotation1948511
version: 1
top level: BBa_I739107
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I739107/annotation1948511/range1948511/1

https://synbiohub.org/public/igem/BBa_I739107/annotation1948511/range1948511/1
type: Range
persistent identity: range1948511
display id: range1948511
version: 1
direction: direction/forward
top level: BBa_I739107
start: 44
end: 78
orientation: inline

R0010
type: SequenceAnnotation
persistent identity: annotation1948512
display id: annotation1948512
version: 1
top level: BBa_I739107
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_I739107/annotation1948512/range1948512/1

https://synbiohub.org/public/igem/BBa_I739107/annotation1948512/range1948512/1
type: Range
persistent identity: range1948512
display id: range1948512
version: 1
direction: direction/forward
top level: BBa_I739107
start: 44
end: 78
orientation: inline

R0051
type: SequenceAnnotation
persistent identity: annotation1948510
display id: annotation1948510
version: 1
top level: BBa_I739107
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I739107/annotation1948510/range1948510/1

https://synbiohub.org/public/igem/BBa_I739107/annotation1948510/range1948510/1
type: Range
persistent identity: range1948510
display id: range1948510
version: 1
direction: direction/forward
top level: BBa_I739107
start: 1
end: 43
orientation: inline

BBa_K1486031
type: ComponentDefinition
persistent identity: BBa K1486031
display id: BBa_K1486031
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CaUra3 selection marker
creator: EPFL iGem team 2014
created: 2014-09-26T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: To edit
mutable notes: To edit
mutable provenance: To edit
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: TEF promoter
sequence annotation: TEF terminator
sequence annotation: Ura3
sequence: https://synbiohub.org/public/igem/BBa_K1486031_sequence/1

TEF promoter
type: SequenceAnnotation
persistent identity: annotation2417785
display id: annotation2417785
version: 1
top level: BBa_K1486031
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1486031/annotation2417785/range2417785/1

https://synbiohub.org/public/igem/BBa_K1486031/annotation2417785/range2417785/1
type: Range
persistent identity: range2417785
display id: range2417785
version: 1
direction: direction/forward
top level: BBa_K1486031
start: 1
end: 344
orientation: inline

TEF terminator
type: SequenceAnnotation
persistent identity: annotation2417787
display id: annotation2417787
version: 1
top level: BBa_K1486031
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1486031/annotation2417787/range2417787/1

https://synbiohub.org/public/igem/BBa_K1486031/annotation2417787/range2417787/1
type: Range
persistent identity: range2417787
display id: range2417787
version: 1
direction: direction/forward
top level: BBa_K1486031
start: 1162
end: 1360
orientation: inline

Ura3
type: SequenceAnnotation
persistent identity: annotation2417786
display id: annotation2417786
version: 1
top level: BBa_K1486031
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1486031/annotation2417786/range2417786/1

https://synbiohub.org/public/igem/BBa_K1486031/annotation2417786/range2417786/1
type: Range
persistent identity: range2417786
display id: range2417786
version: 1
direction: direction/forward
top level: BBa_K1486031
start: 345
end: 1157
orientation: inline

BBa_K089005
type: ComponentDefinition
persistent identity: BBa K089005
display id: BBa_K089005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: -35 to Tc start site of phaC
creator: Trent Mortensen
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:08:37Z
discontinued: false
dominant: false
group u list: _245_
m user id: 0
owner id: 3156
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: -35 to Tc start site of phaC
mutable notes: very small PCR porduct
mutable provenance: Ralstonia eutropha genomic DNA
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -10 site
sequence annotation: +1 site
sequence annotation: -35 site
sequence: https://synbiohub.org/public/igem/BBa_K089005_sequence/1

-10 site
type: SequenceAnnotation
persistent identity: annotation1996707
display id: annotation1996707
version: 1
top level: BBa_K089005
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K089005/annotation1996707/range1996707/1

https://synbiohub.org/public/igem/BBa_K089005/annotation1996707/range1996707/1
type: Range
persistent identity: range1996707
display id: range1996707
version: 1
direction: direction/forward
top level: BBa_K089005
start: 34
end: 39
orientation: inline

+1 site
type: SequenceAnnotation
persistent identity: annotation1996714
display id: annotation1996714
version: 1
top level: BBa_K089005
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K089005/annotation1996714/range1996714/1

https://synbiohub.org/public/igem/BBa_K089005/annotation1996714/range1996714/1
type: Range
persistent identity: range1996714
display id: range1996714
version: 1
direction: direction/forward
top level: BBa_K089005
start: 45
end: 45
orientation: inline

-35 site
type: SequenceAnnotation
persistent identity: annotation1996684
display id: annotation1996684
version: 1
top level: BBa_K089005
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K089005/annotation1996684/range1996684/1

https://synbiohub.org/public/igem/BBa_K089005/annotation1996684/range1996684/1
type: Range
persistent identity: range1996684
display id: range1996684
version: 1
direction: direction/forward
top level: BBa_K089005
start: 9
end: 14
orientation: inline

mRFP1
type: ComponentDefinition
persistent identity: BBa K678032
display id: BBa_K678032
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: mRFP1-TS
creator: DTU-Denmark-2
created: 2011-09-08T11:00:00Z
modified: 2015-05-08T01:13:04Z
discontinued: false
dominant: false
experience: experience/None
group u list: _882_
m user id: 0
owner id: 8341
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Monomeric red fluorescent protein (mRFP1) is a derivative of the red fluorescent protein cloned from Discosoma coral (DsRed) (1). The successful use of mRFP1 in Aspergillus nidulans has previously been described (2).
mutable notes: This part was designed for the Plug'n'Play with DNA assembly standard (RFC 80), therefore this part contains tails at the 5' and 3' ends as shown on the figure below. Please notice that mRFP1-TS is not the same as BBa_K678030 or BBa_K678031.
mutable provenance: From p71-mRFP1 of the Center for Microbial Biotechnology plasmid collection at the Technical University of Denmark
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: Linker 3
sequence annotation: RFP
sequence: https://synbiohub.org/public/igem/BBa_K678032_sequence/1

Linker 3
type: SequenceAnnotation
persistent identity: annotation2127393
display id: annotation2127393
version: 1
top level: mRFP1
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K678032/annotation2127393/range2127393/1

https://synbiohub.org/public/igem/BBa_K678032/annotation2127393/range2127393/1
type: Range
persistent identity: range2127393
display id: range2127393
version: 1
direction: direction/forward
top level: mRFP1
start: 1
end: 9
orientation: inline

RFP
type: SequenceAnnotation
persistent identity: annotation2127394
display id: annotation2127394
version: 1
top level: mRFP1
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K678032/annotation2127394/range2127394/1

https://synbiohub.org/public/igem/BBa_K678032/annotation2127394/range2127394/1
type: Range
persistent identity: range2127394
display id: range2127394
version: 1
direction: direction/forward
top level: mRFP1
start: 10
end: 687
orientation: inline

P43
type: ComponentDefinition
persistent identity: BBa K143013
display id: BBa_K143013
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter 43 a constitutive promoter for B. subtilis
creator: James Chappell
created: 2008-09-10T11:00:00Z
modified: 2015-05-08T01:10:23Z
discontinued: false
dominant: false
experience: experience/None
group u list: _199_
m user id: 0
owner id: 2090
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Promoter 43 is a constitutive promoter that constitutively expresses the P43 protein in ''B.subtilis''. This promoter has been shown to be recognized and active during the exponential and lag phases of growth. It has been hypothesized that the ability to recognize the promoter in exponential and lag phase of growth is due to the recognition of the promoter by both sigma factor 55 (the major sigma factor) and sigma factor 37 (the lag phase sigma factor) 1. The P43 promoter has been previously used for constitutive expression of exogenous genes within ''B.subtilis'' vectors 2. The context with which we used the promoter P43 is as a '''Polymerase Per Second''' (PoPS) generator.
mutable notes: The biobrick part was designed to include the binding sites for both the sigma factor A and B. In addition the biobrick standard was applied to the promoter P43 sequence.
mutable provenance: The part was designed using the sequence from the ''B.subtilis'' genome and from previously published papers 2. This sequence was then synthesised by Geneart.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: Sigma B -35
sequence annotation: Sigma A -35
sequence annotation: Sigma B -10
sequence annotation: Sigma A -10
sequence: https://synbiohub.org/public/igem/BBa_K143013_sequence/1

Sigma B -35
type: SequenceAnnotation
persistent identity: annotation1975706
display id: annotation1975706
version: 1
top level: P43
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K143013/annotation1975706/range1975706/1

https://synbiohub.org/public/igem/BBa_K143013/annotation1975706/range1975706/1
type: Range
persistent identity: range1975706
display id: range1975706
version: 1
direction: direction/forward
top level: P43
start: 14
end: 22
orientation: inline

Sigma A -35
type: SequenceAnnotation
persistent identity: annotation1975708
display id: annotation1975708
version: 1
top level: P43
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K143013/annotation1975708/range1975708/1

https://synbiohub.org/public/igem/BBa_K143013/annotation1975708/range1975708/1
type: Range
persistent identity: range1975708
display id: range1975708
version: 1
direction: direction/forward
top level: P43
start: 24
end: 29
orientation: inline

Sigma B -10
type: SequenceAnnotation
persistent identity: annotation1975707
display id: annotation1975707
version: 1
top level: P43
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K143013/annotation1975707/range1975707/1

https://synbiohub.org/public/igem/BBa_K143013/annotation1975707/range1975707/1
type: Range
persistent identity: range1975707
display id: range1975707
version: 1
direction: direction/forward
top level: P43
start: 38
end: 47
orientation: inline

Sigma A -10
type: SequenceAnnotation
persistent identity: annotation1975709
display id: annotation1975709
version: 1
top level: P43
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K143013/annotation1975709/range1975709/1

https://synbiohub.org/public/igem/BBa_K143013/annotation1975709/range1975709/1
type: Range
persistent identity: range1975709
display id: range1975709
version: 1
direction: direction/forward
top level: P43
start: 47
end: 52
orientation: inline

BBa_K678041
type: ComponentDefinition
persistent identity: BBa K678041
display id: BBa_K678041
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pyrG-DR cassette, for selection of Aspergillus nidulans
creator: DTU-Denmark-2
created: 2011-09-03T11:00:00Z
modified: 2015-05-08T01:13:04Z
discontinued: false
dominant: false
experience: experience/None
group u list: _882_
m user id: 0
owner id: 8341
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: pyrG-DR cassette with directed repeats, for selection of Aspergillus nidulans
mutable notes: Compatible with the Plug'n'Play assembly standard
mutable provenance: pDel2
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Linker 5
sequence annotation: pyrG-DR cassette
sequence: https://synbiohub.org/public/igem/BBa_K678041_sequence/1

Linker 5
type: SequenceAnnotation
persistent identity: annotation2127413
display id: annotation2127413
version: 1
top level: BBa_K678041
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K678041/annotation2127413/range2127413/1

https://synbiohub.org/public/igem/BBa_K678041/annotation2127413/range2127413/1
type: Range
persistent identity: range2127413
display id: range2127413
version: 1
direction: direction/forward
top level: BBa_K678041
start: 1
end: 8
orientation: inline

pyrG-DR cassette
type: SequenceAnnotation
persistent identity: annotation2127414
display id: annotation2127414
version: 1
top level: BBa_K678041
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K678041/annotation2127414/range2127414/1

https://synbiohub.org/public/igem/BBa_K678041/annotation2127414/range2127414/1
type: Range
persistent identity: range2127414
display id: range2127414
version: 1
direction: direction/forward
top level: BBa_K678041
start: 9
end: 2168
orientation: inline

BBa_K1486014
type: ComponentDefinition
persistent identity: BBa K1486014
display id: BBa_K1486014
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: IFP[1]-CpxR under pBAD
creator: iGem EPFL 2014
created: 2014-08-31T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part has a fused protein downstream of an arabinose-inducible promoter: IFP[1]-CpxR. Biobrick BBa_K1486015 has IFP[2]-CpxR with an arabinose promoter. This construct was made in both orientations: BBa_K1486012 is CpxR-IFP[1] (with an arabinose promoter).
mutable notes: Both orientations were made, as well as for IFP[2] so all dimerizations could be tested (see main page for biobrick numbers). Flexible linkers were used to fuse both proteins, and an RBS was put between the promoter and the cds.
mutable provenance: to edit
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0034
component: BBa_K1486000
component: BBa_K1486004
component: BBa_K1486006
component: BBa_I0500
sequence annotation: BBa_I0500
sequence annotation: BBa_K1486004
sequence annotation: BBa_B0034
sequence annotation: BBa_K1486006
sequence annotation: BBa_K1486000
sequence: https://synbiohub.org/public/igem/BBa_K1486014_sequence/1

BBa_B0034
type: Component
persistent identity: component2424738
display id: component2424738
version: 1
top level: BBa_K1486014
access: public
definition: BBa_B0034

BBa_K1486000
type: Component
persistent identity: component2424747
display id: component2424747
version: 1
top level: BBa_K1486014
access: public
definition: BBa_K1486000

BBa_K1486004
type: Component
persistent identity: component2424743
display id: component2424743
version: 1
top level: BBa_K1486014
access: public
definition: BBa_K1486004

BBa_K1486006
type: Component
persistent identity: component2424742
display id: component2424742
version: 1
top level: BBa_K1486014
access: public
definition: BBa_K1486006

BBa_I0500
type: Component
persistent identity: component2424736
display id: component2424736
version: 1
top level: BBa_K1486014
access: public
definition: pBad/araC

BBa_I0500
type: SequenceAnnotation
persistent identity: annotation2424736
display id: annotation2424736
version: 1
top level: BBa_K1486014
location: https://synbiohub.org/public/igem/BBa_K1486014/annotation2424736/range2424736/1
component: BBa_I0500

https://synbiohub.org/public/igem/BBa_K1486014/annotation2424736/range2424736/1
type: Range
persistent identity: range2424736
display id: range2424736
version: 1
direction: direction/reverse
top level: BBa_K1486014
start: 1
end: 1210
orientation: inline

BBa_K1486004
type: SequenceAnnotation
persistent identity: annotation2424743
display id: annotation2424743
version: 1
top level: BBa_K1486014
location: https://synbiohub.org/public/igem/BBa_K1486014/annotation2424743/range2424743/1
component: BBa_K1486004

https://synbiohub.org/public/igem/BBa_K1486014/annotation2424743/range2424743/1
type: Range
persistent identity: range2424743
display id: range2424743
version: 1
direction: direction/reverse
top level: BBa_K1486014
start: 1628
end: 1657
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2424738
display id: annotation2424738
version: 1
top level: BBa_K1486014
location: https://synbiohub.org/public/igem/BBa_K1486014/annotation2424738/range2424738/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1486014/annotation2424738/range2424738/1
type: Range
persistent identity: range2424738
display id: range2424738
version: 1
direction: direction/reverse
top level: BBa_K1486014
start: 1211
end: 1222
orientation: inline

BBa_K1486006
type: SequenceAnnotation
persistent identity: annotation2424742
display id: annotation2424742
version: 1
top level: BBa_K1486014
location: https://synbiohub.org/public/igem/BBa_K1486014/annotation2424742/range2424742/1
component: BBa_K1486006

https://synbiohub.org/public/igem/BBa_K1486014/annotation2424742/range2424742/1
type: Range
persistent identity: range2424742
display id: range2424742
version: 1
direction: direction/reverse
top level: BBa_K1486014
start: 1223
end: 1627
orientation: inline

BBa_K1486000
type: SequenceAnnotation
persistent identity: annotation2424747
display id: annotation2424747
version: 1
top level: BBa_K1486014
location: https://synbiohub.org/public/igem/BBa_K1486014/annotation2424747/range2424747/1
component: BBa_K1486000

https://synbiohub.org/public/igem/BBa_K1486014/annotation2424747/range2424747/1
type: Range
persistent identity: range2424747
display id: range2424747
version: 1
direction: direction/reverse
top level: BBa_K1486014
start: 1658
end: 2359
orientation: inline

BBa_I741018
type: ComponentDefinition
persistent identity: BBa I741018
display id: BBa_I741018
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Right facing promoter (for xylF) controlled by xylR and CRP-cAMP
creator: Lucien Weiss
created: 2007-06-20T11:00:00Z
modified: 2015-08-31T04:08:01Z
discontinued: false
dominant: false
experience: experience/Issues
group u list: _143_
m user id: 0
owner id: 1763
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: potential looping feature possibly removed
mutable notes: removed RBS, left promoter, and several xylR binding sites
mutable provenance: ecoli k12 chromosome
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: xylR activator binding site
sequence annotation: Promoter: xylFp
sequence annotation: CRP-cAMP duel regulator binding site
sequence annotation: +1
sequence: https://synbiohub.org/public/igem/BBa_I741018_sequence/1

xylR activator binding site
type: SequenceAnnotation
persistent identity: annotation1935201
display id: annotation1935201
version: 1
top level: BBa_I741018
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I741018/annotation1935201/range1935201/1

https://synbiohub.org/public/igem/BBa_I741018/annotation1935201/range1935201/1
type: Range
persistent identity: range1935201
display id: range1935201
version: 1
direction: direction/forward
top level: BBa_I741018
start: 146
end: 162
orientation: inline

xylR activator binding site
type: SequenceAnnotation
persistent identity: annotation1935199
display id: annotation1935199
version: 1
top level: BBa_I741018
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I741018/annotation1935199/range1935199/1

https://synbiohub.org/public/igem/BBa_I741018/annotation1935199/range1935199/1
type: Range
persistent identity: range1935199
display id: range1935199
version: 1
direction: direction/forward
top level: BBa_I741018
start: 125
end: 142
orientation: inline

Promoter: xylFp
type: SequenceAnnotation
persistent identity: annotation1935200
display id: annotation1935200
version: 1
top level: BBa_I741018
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I741018/annotation1935200/range1935200/1

https://synbiohub.org/public/igem/BBa_I741018/annotation1935200/range1935200/1
type: Range
persistent identity: range1935200
display id: range1935200
version: 1
direction: direction/forward
top level: BBa_I741018
start: 138
end: 215
orientation: inline

CRP-cAMP duel regulator binding site
type: SequenceAnnotation
persistent identity: annotation1935203
display id: annotation1935203
version: 1
top level: BBa_I741018
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I741018/annotation1935203/range1935203/1

https://synbiohub.org/public/igem/BBa_I741018/annotation1935203/range1935203/1
type: Range
persistent identity: range1935203
display id: range1935203
version: 1
direction: direction/forward
top level: BBa_I741018
start: 4
end: 25
orientation: inline

+1
type: SequenceAnnotation
persistent identity: annotation1935202
display id: annotation1935202
version: 1
top level: BBa_I741018
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I741018/annotation1935202/range1935202/1

https://synbiohub.org/public/igem/BBa_I741018/annotation1935202/range1935202/1
type: Range
persistent identity: range1935202
display id: range1935202
version: 1
direction: direction/forward
top level: BBa_I741018
start: 195
end: 195
orientation: inline

BBa_J23112
type: ComponentDefinition
persistent identity: BBa J23112
display id: BBa_J23112
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: constitutive promoter family member
creator: John Anderson
created: 2006-08-16T11:00:00Z
modified: 2015-08-31T04:08:40Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: Later
mutable notes: N/A
mutable provenance: Later
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J23112_sequence/1

p_qrr4
type: ComponentDefinition
persistent identity: BBa K131017
display id: BBa_K131017
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: p_qrr4 from Vibrio harveyi
creator: Sonja Georgijevic
created: 2008-10-27T12:00:00Z
modified: 2015-05-08T01:09:47Z
discontinued: false
dominant: true
experience: experience/None
group u list: _259_
m user id: 0
owner id: 1653
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: qrr4 promoter from Vibrio harveyi
mutable notes: 5' upstream sequence of qrr4 containing -12 and -24 regions (specific for Sigma factor 54)
mutable provenance: >gi|156527546:c1833759-1833376 Vibrio harveyi ATCC BAA-1116 chromosome II, complete sequence
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -24 region
sequence annotation: -12 region
sequence: https://synbiohub.org/public/igem/BBa_K131017_sequence/1

-24 region
type: SequenceAnnotation
persistent identity: annotation1990436
display id: annotation1990436
version: 1
top level: p_qrr4
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K131017/annotation1990436/range1990436/1

https://synbiohub.org/public/igem/BBa_K131017/annotation1990436/range1990436/1
type: Range
persistent identity: range1990436
display id: range1990436
version: 1
direction: direction/forward
top level: p_qrr4
start: 250
end: 254
orientation: inline

-12 region
type: SequenceAnnotation
persistent identity: annotation1990440
display id: annotation1990440
version: 1
top level: p_qrr4
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K131017/annotation1990440/range1990440/1

https://synbiohub.org/public/igem/BBa_K131017/annotation1990440/range1990440/1
type: Range
persistent identity: range1990440
display id: range1990440
version: 1
direction: direction/forward
top level: p_qrr4
start: 262
end: 264
orientation: inline

BBa_K112402
type: ComponentDefinition
persistent identity: BBa K112402
display id: BBa_K112402
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: promoter for FabA gene - Membrane Damage and Ultrasound Senstitive
creator: Christina Brown
created: 2008-10-20T11:00:00Z
modified: 2015-05-08T01:09:17Z
discontinued: false
dominant: false
group u list: _224_
m user id: 0
owner id: 3241
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: According to Vollmer, et. al in their 1998 Applied and Enviormental Microbiology paper, "Bacterial Stress Responses to 1-Megahertz Pulsed Ultrasound in the Presence of Microbubble", the promoter for the fabA gene, a known membrane damage response gene, is upregulated seven fold when cells are exposed to high frequency ultrasound and microbubble cavitation.
mutable notes: none
mutable provenance: cloned from E. coli MG1655 genome
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K112402_sequence/1

BBa_K1486015
type: ComponentDefinition
persistent identity: BBa K1486015
display id: BBa_K1486015
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: IFP[2]-CpxR under pBAD
creator: EPFL iGem team 2014
created: 2014-09-09T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part has a fused protein downstream of an arabinose-inducible promoter: IFP[2]-CpxR. Biobrick BBa_K1486014 has IFP[1]-CpxR with an arabinose promoter. This construct was made in both orientations: BBa_K1486013 is CpxR-IFP[2] (with an arabinose promoter).
mutable notes: Both orientations were made, as well as for IFP[1] so all dimerizations could be tested (see main page for biobrick numbers). Flexible linkers were used to fuse both proteins, and an RBS was put before each one of them.
mutable provenance: To edit.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K1486000
component: BBa_B0034
component: BBa_K1486004
component: BBa_K1486007
component: BBa_I0500
sequence annotation: BBa_K1486007
sequence annotation: BBa_I0500
sequence annotation: BBa_K1486000
sequence annotation: BBa_K1486004
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K1486015_sequence/1

BBa_K1486000
type: Component
persistent identity: component2382859
display id: component2382859
version: 1
top level: BBa_K1486015
access: public
definition: BBa_K1486000

BBa_B0034
type: Component
persistent identity: component2382850
display id: component2382850
version: 1
top level: BBa_K1486015
access: public
definition: BBa_B0034

BBa_K1486004
type: Component
persistent identity: component2382855
display id: component2382855
version: 1
top level: BBa_K1486015
access: public
definition: BBa_K1486004

BBa_K1486007
type: Component
persistent identity: component2382854
display id: component2382854
version: 1
top level: BBa_K1486015
access: public
definition: BBa_K1486007

BBa_I0500
type: Component
persistent identity: component2382848
display id: component2382848
version: 1
top level: BBa_K1486015
access: public
definition: pBad/araC

BBa_K1486007
type: SequenceAnnotation
persistent identity: annotation2382854
display id: annotation2382854
version: 1
top level: BBa_K1486015
location: https://synbiohub.org/public/igem/BBa_K1486015/annotation2382854/range2382854/1
component: BBa_K1486007

https://synbiohub.org/public/igem/BBa_K1486015/annotation2382854/range2382854/1
type: Range
persistent identity: range2382854
display id: range2382854
version: 1
direction: direction/reverse
top level: BBa_K1486015
start: 1237
end: 1815
orientation: inline

BBa_I0500
type: SequenceAnnotation
persistent identity: annotation2382848
display id: annotation2382848
version: 1
top level: BBa_K1486015
location: https://synbiohub.org/public/igem/BBa_K1486015/annotation2382848/range2382848/1
component: BBa_I0500

https://synbiohub.org/public/igem/BBa_K1486015/annotation2382848/range2382848/1
type: Range
persistent identity: range2382848
display id: range2382848
version: 1
direction: direction/reverse
top level: BBa_K1486015
start: 1
end: 1210
orientation: inline

BBa_K1486000
type: SequenceAnnotation
persistent identity: annotation2382859
display id: annotation2382859
version: 1
top level: BBa_K1486015
location: https://synbiohub.org/public/igem/BBa_K1486015/annotation2382859/range2382859/1
component: BBa_K1486000

https://synbiohub.org/public/igem/BBa_K1486015/annotation2382859/range2382859/1
type: Range
persistent identity: range2382859
display id: range2382859
version: 1
direction: direction/reverse
top level: BBa_K1486015
start: 1860
end: 2561
orientation: inline

BBa_K1486004
type: SequenceAnnotation
persistent identity: annotation2382855
display id: annotation2382855
version: 1
top level: BBa_K1486015
location: https://synbiohub.org/public/igem/BBa_K1486015/annotation2382855/range2382855/1
component: BBa_K1486004

https://synbiohub.org/public/igem/BBa_K1486015/annotation2382855/range2382855/1
type: Range
persistent identity: range2382855
display id: range2382855
version: 1
direction: direction/reverse
top level: BBa_K1486015
start: 1824
end: 1853
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2382850
display id: annotation2382850
version: 1
top level: BBa_K1486015
location: https://synbiohub.org/public/igem/BBa_K1486015/annotation2382850/range2382850/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1486015/annotation2382850/range2382850/1
type: Range
persistent identity: range2382850
display id: range2382850
version: 1
direction: direction/reverse
top level: BBa_K1486015
start: 1219
end: 1230
orientation: inline

BBa_K137091
type: ComponentDefinition
persistent identity: BBa K137091
display id: BBa_K137091
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: optimized (A) repeat constitutive promoter with 18 bp between -10 and -35 elements
creator: Allen Lin
created: 2008-08-20T11:00:00Z
modified: 2015-05-08T01:10:10Z
discontinued: false
dominant: false
experience: experience/None
group u list: _187_
m user id: 0
owner id: 3112
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: optimized (A) repeat constitutive promoter with 18 bp between -10 and -35 elements
mutable notes: None
mutable provenance: PCR
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K137091_sequence/1

BBa_J23110
type: ComponentDefinition
persistent identity: BBa J23110
display id: BBa_J23110
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: constitutive promoter family member
creator: John Anderson
created: 2006-08-16T11:00:00Z
modified: 2015-08-31T04:08:40Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: true
mutable description: Later
mutable notes: N/A
mutable provenance: Later
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J23110_sequence/1

BBa_M13511
type: ComponentDefinition
persistent identity: BBa M13511
display id: BBa_M13511
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: M13K07 gene XI RBS
creator: Natalie Kuldell
created: 2006-12-14T12:00:00Z
modified: 2015-05-08T01:13:56Z
discontinued: false
dominant: false
experience: experience/None
group u list: _45_
m user id: 0
owner id: 314
owning group id: 1
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This RBS is part of the bacteriophage M13 genome, initiating translation of the gene XI message (BBa_M13011). The part aligns with base pairs 3900-3915 in M13K07 genome. It was NOT identified as the RBS for gene XI message by Wezenbeek et al in Gene (1980) 11: 129-148
mutable notes: none
mutable provenance: New England Biolabs M13K07. Sequenced in 2002 by L. Panganaban
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_M13511_sequence/1

BBa_J64800
type: ComponentDefinition
persistent identity: BBa J64800
display id: BBa_J64800
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RHLR/RHLI Inducible & LasR/LasI repressible Promoter
creator: laura lavery
created: 2007-03-24T12:00:00Z
modified: 2015-05-08T01:08:17Z
discontinued: false
dominant: false
experience: experience/None
group u list: _98_
m user id: 0
owner id: 1431
owning group id: 98
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This part is similar to BBa_R0079 but has an added binding site for RHLR/RHLI in between the -35 and -10 region. Thus this promoter is induced by LasR/LasI but repressed by RHLR/RHLi binding.
mutable notes: There is a 17bp spacer between the -35 and -10 regions and the binding site for RHLR/RHLI is 20bp long. I was able to fit the whole binding site in the spacer region because the last three base pairs of the -35 region and the first three base pairs of the RHLR/RHLI binding site are the same.
mutable provenance: Rust L. et. al., "Analysis of the Pseudomonas aeruginosa Elastase (lasB) Regulatory Region". Journal of Bacteriology, 1996.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: LasR/LasI
sequence annotation: -35
sequence annotation: -10
sequence annotation: RHLR/RHLI
sequence: https://synbiohub.org/public/igem/BBa_J64800_sequence/1

LasR/LasI
type: SequenceAnnotation
persistent identity: annotation1921061
display id: annotation1921061
version: 1
top level: BBa_J64800
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_J64800/annotation1921061/range1921061/1

https://synbiohub.org/public/igem/BBa_J64800/annotation1921061/range1921061/1
type: Range
persistent identity: range1921061
display id: range1921061
version: 1
direction: direction/forward
top level: BBa_J64800
start: 23
end: 40
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1921062
display id: annotation1921062
version: 1
top level: BBa_J64800
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J64800/annotation1921062/range1921062/1

https://synbiohub.org/public/igem/BBa_J64800/annotation1921062/range1921062/1
type: Range
persistent identity: range1921062
display id: range1921062
version: 1
direction: direction/forward
top level: BBa_J64800
start: 19
end: 24
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1921063
display id: annotation1921063
version: 1
top level: BBa_J64800
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J64800/annotation1921063/range1921063/1

https://synbiohub.org/public/igem/BBa_J64800/annotation1921063/range1921063/1
type: Range
persistent identity: range1921063
display id: range1921063
version: 1
direction: direction/forward
top level: BBa_J64800
start: 42
end: 47
orientation: inline

RHLR/RHLI
type: SequenceAnnotation
persistent identity: annotation1921060
display id: annotation1921060
version: 1
top level: BBa_J64800
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_J64800/annotation1921060/range1921060/1

https://synbiohub.org/public/igem/BBa_J64800/annotation1921060/range1921060/1
type: Range
persistent identity: range1921060
display id: range1921060
version: 1
direction: direction/forward
top level: BBa_J64800
start: 1
end: 23
orientation: inline

pJEJAM18
type: ComponentDefinition
persistent identity: BBa K678066
display id: BBa_K678066
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pJEJAM18
creator: DTU-Denmark-2
created: 2011-09-12T11:00:00Z
modified: 2015-05-08T01:13:05Z
discontinued: false
dominant: false
experience: experience/None
group u list: _882_
m user id: 0
owner id: 8341
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: To be announced
mutable notes: To be announced
mutable provenance: To be announced
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000155
role: partType/Plasmid
component: BBa_K678044
component: BBa_K678032
component: BBa_K678000
component: BBa_K678046
component: BBa_K678038
component: BBa_K678028
sequence annotation: BBa_K678044
sequence annotation: BBa_K678028
sequence annotation: BBa_K678046
sequence annotation: BBa_K678000
sequence annotation: BBa_K678032
sequence annotation: BBa_K678038
sequence: https://synbiohub.org/public/igem/BBa_K678066_sequence/1

BBa_K678044
type: Component
persistent identity: component2127602
display id: component2127602
version: 1
top level: pJEJAM18
access: public
definition: bleR

BBa_K678032
type: Component
persistent identity: component2127596
display id: component2127596
version: 1
top level: pJEJAM18
access: public
definition: mRFP1

BBa_K678000
type: Component
persistent identity: component2127590
display id: component2127590
version: 1
top level: pJEJAM18
access: public
definition: DMKP-P6

BBa_K678046
type: Component
persistent identity: component2127605
display id: component2127605
version: 1
top level: pJEJAM18
access: public
definition: pFun

BBa_K678038
type: Component
persistent identity: component2127599
display id: component2127599
version: 1
top level: pJEJAM18
access: public
definition: BBa_K678038

BBa_K678028
type: Component
persistent identity: component2127593
display id: component2127593
version: 1
top level: pJEJAM18
access: public
definition: GFP-GOI

BBa_K678044
type: SequenceAnnotation
persistent identity: annotation2127602
display id: annotation2127602
version: 1
top level: pJEJAM18
location: https://synbiohub.org/public/igem/BBa_K678066/annotation2127602/range2127602/1
component: BBa_K678044

https://synbiohub.org/public/igem/BBa_K678066/annotation2127602/range2127602/1
type: Range
persistent identity: range2127602
display id: range2127602
version: 1
direction: direction/reverse
top level: pJEJAM18
start: 2983
end: 4645
orientation: inline

BBa_K678028
type: SequenceAnnotation
persistent identity: annotation2127593
display id: annotation2127593
version: 1
top level: pJEJAM18
location: https://synbiohub.org/public/igem/BBa_K678066/annotation2127593/range2127593/1
component: BBa_K678028

https://synbiohub.org/public/igem/BBa_K678066/annotation2127593/range2127593/1
type: Range
persistent identity: range2127593
display id: range2127593
version: 1
direction: direction/reverse
top level: pJEJAM18
start: 557
end: 1281
orientation: inline

BBa_K678046
type: SequenceAnnotation
persistent identity: annotation2127605
display id: annotation2127605
version: 1
top level: pJEJAM18
location: https://synbiohub.org/public/igem/BBa_K678066/annotation2127605/range2127605/1
component: BBa_K678046

https://synbiohub.org/public/igem/BBa_K678066/annotation2127605/range2127605/1
type: Range
persistent identity: range2127605
display id: range2127605
version: 1
direction: direction/reverse
top level: pJEJAM18
start: 4654
end: 7531
orientation: inline

BBa_K678000
type: SequenceAnnotation
persistent identity: annotation2127590
display id: annotation2127590
version: 1
top level: pJEJAM18
location: https://synbiohub.org/public/igem/BBa_K678066/annotation2127590/range2127590/1
component: BBa_K678000

https://synbiohub.org/public/igem/BBa_K678066/annotation2127590/range2127590/1
type: Range
persistent identity: range2127590
display id: range2127590
version: 1
direction: direction/reverse
top level: pJEJAM18
start: 1
end: 548
orientation: inline

BBa_K678032
type: SequenceAnnotation
persistent identity: annotation2127596
display id: annotation2127596
version: 1
top level: pJEJAM18
location: https://synbiohub.org/public/igem/BBa_K678066/annotation2127596/range2127596/1
component: BBa_K678032

https://synbiohub.org/public/igem/BBa_K678066/annotation2127596/range2127596/1
type: Range
persistent identity: range2127596
display id: range2127596
version: 1
direction: direction/reverse
top level: pJEJAM18
start: 1290
end: 1976
orientation: inline

BBa_K678038
type: SequenceAnnotation
persistent identity: annotation2127599
display id: annotation2127599
version: 1
top level: pJEJAM18
location: https://synbiohub.org/public/igem/BBa_K678066/annotation2127599/range2127599/1
component: BBa_K678038

https://synbiohub.org/public/igem/BBa_K678066/annotation2127599/range2127599/1
type: Range
persistent identity: range2127599
display id: range2127599
version: 1
direction: direction/reverse
top level: pJEJAM18
start: 1985
end: 2974
orientation: inline

BBa_K678037
type: ComponentDefinition
persistent identity: BBa K678037
display id: BBa_K678037
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: T1-motni, terminator for Aspergillus niger
creator: DTU-Denmark-2
created: 2011-09-03T11:00:00Z
modified: 2015-05-08T01:13:04Z
discontinued: false
dominant: false
experience: experience/None
group u list: _882_
m user id: 0
owner id: 8341
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: T1-motni is terminator that works in the fungus Aspergillus niger
mutable notes: Compatible with the Plug'n'Play assembly standard
mutable provenance: Aspergillus niger genomic DNA
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: Linker 4
sequence annotation: T1-motni terminator
sequence: https://synbiohub.org/public/igem/BBa_K678037_sequence/1

Linker 4
type: SequenceAnnotation
persistent identity: annotation2127406
display id: annotation2127406
version: 1
top level: BBa_K678037
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K678037/annotation2127406/range2127406/1

https://synbiohub.org/public/igem/BBa_K678037/annotation2127406/range2127406/1
type: Range
persistent identity: range2127406
display id: range2127406
version: 1
direction: direction/forward
top level: BBa_K678037
start: 1
end: 8
orientation: inline

T1-motni terminator
type: SequenceAnnotation
persistent identity: annotation2127405
display id: annotation2127405
version: 1
top level: BBa_K678037
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K678037/annotation2127405/range2127405/1

https://synbiohub.org/public/igem/BBa_K678037/annotation2127405/range2127405/1
type: Range
persistent identity: range2127405
display id: range2127405
version: 1
direction: direction/forward
top level: BBa_K678037
start: 9
end: 1006
orientation: inline

LacZ promo
type: ComponentDefinition
persistent identity: BBa K119000
display id: BBa_K119000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Constitutive weak promoter of lacZ
creator: Miguel Angel Ramirez-Romero
created: 2008-09-27T11:00:00Z
modified: 2015-05-08T01:09:38Z
discontinued: false
dominant: false
experience: experience/None
group u list: _235_
m user id: 0
owner id: 3054
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: LacZ constitutive promoter, without including operators.
mutable notes: We needed two versions of it.
mutable provenance: Lac operon from E. coli.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: LacZ promoter
sequence: https://synbiohub.org/public/igem/BBa_K119000_sequence/1

LacZ promoter
type: SequenceAnnotation
persistent identity: annotation1976843
display id: annotation1976843
version: 1
top level: LacZ promo
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K119000/annotation1976843/range1976843/1

https://synbiohub.org/public/igem/BBa_K119000/annotation1976843/range1976843/1
type: Range
persistent identity: range1976843
display id: range1976843
version: 1
direction: direction/forward
top level: LacZ promo
start: 1
end: 38
orientation: inline

BBa_K110012
type: ComponentDefinition
persistent identity: BBa K110012
display id: BBa_K110012
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: STE2 terminator
creator: James DiCarlo
created: 2008-08-26T11:00:00Z
modified: 2015-05-08T01:09:09Z
discontinued: false
dominant: false
experience: experience/Issues
group u list: _201_
m user id: 0
owner id: 2669
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is the exact region between the end of the ORF of STE2-W and the beginning of BST1-C
mutable notes: This sequence was taken from the yeast genome
mutable provenance: SGD
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: Terminator
sequence: https://synbiohub.org/public/igem/BBa_K110012_sequence/1

Terminator
type: SequenceAnnotation
persistent identity: annotation1994877
display id: annotation1994877
version: 1
top level: BBa_K110012
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K110012/annotation1994877/range1994877/1

https://synbiohub.org/public/igem/BBa_K110012/annotation1994877/range1994877/1
type: Range
persistent identity: range1994877
display id: range1994877
version: 1
direction: direction/forward
top level: BBa_K110012
start: 1
end: 123
orientation: inline

U097NUL+D0
type: ComponentDefinition
persistent identity: BBa I732408
display id: BBa_I732408
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Family Member (U097NUL+D014O22)
creator: Zhan Jian
created: 2007-09-19T11:00:00Z
modified: 2015-08-31T04:07:58Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1572
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: A Double Reporter (LacZ-alpha and RFP) loaded on pSB3C5. For more information please refer to BBa_I732402.
mutable notes: Might be later edited.
mutable provenance: Might be later edited.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: BBa_I732006
sequence annotation: polya
sequence annotation: mrfp1
sequence annotation: stem_loop
sequence annotation: BBa_E1010
sequence annotation: BglII
sequence annotation: START
sequence annotation: BBa_I732007
sequence annotation: BBa_B0034
sequence annotation: lacZ
sequence annotation: barcode
sequence annotation: BamHI
sequence annotation: T7 TE
sequence annotation: conserved
sequence annotation: stop
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: STOP
sequence: https://synbiohub.org/public/igem/BBa_I732408_sequence/1

BBa_I732006
type: SequenceAnnotation
persistent identity: annotation1944505
display id: annotation1944505
version: 1
top level: U097NUL+D0
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_I732408/annotation1944505/range1944505/1

https://synbiohub.org/public/igem/BBa_I732408/annotation1944505/range1944505/1
type: Range
persistent identity: range1944505
display id: range1944505
version: 1
direction: direction/reverse
top level: U097NUL+D0
start: 46
end: 279
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1944515
display id: annotation1944515
version: 1
top level: U097NUL+D0
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_I732408/annotation1944515/range1944515/1

https://synbiohub.org/public/igem/BBa_I732408/annotation1944515/range1944515/1
type: Range
persistent identity: range1944515
display id: range1944515
version: 1
direction: direction/forward
top level: U097NUL+D0
start: 1135
end: 1148
orientation: inline

mrfp1
type: SequenceAnnotation
persistent identity: annotation1944508
display id: annotation1944508
version: 1
top level: U097NUL+D0
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_I732408/annotation1944508/range1944508/1

https://synbiohub.org/public/igem/BBa_I732408/annotation1944508/range1944508/1
type: Range
persistent identity: range1944508
display id: range1944508
version: 1
direction: direction/forward
top level: U097NUL+D0
start: 306
end: 980
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation1944512
display id: annotation1944512
version: 1
top level: U097NUL+D0
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_I732408/annotation1944512/range1944512/1

https://synbiohub.org/public/igem/BBa_I732408/annotation1944512/range1944512/1
type: Range
persistent identity: range1944512
display id: range1944512
version: 1
direction: direction/forward
top level: U097NUL+D0
start: 1031
end: 1074
orientation: inline

BBa_E1010
type: SequenceAnnotation
persistent identity: annotation1944509
display id: annotation1944509
version: 1
top level: U097NUL+D0
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_I732408/annotation1944509/range1944509/1

https://synbiohub.org/public/igem/BBa_I732408/annotation1944509/range1944509/1
type: Range
persistent identity: range1944509
display id: range1944509
version: 1
direction: direction/reverse
top level: U097NUL+D0
start: 306
end: 986
orientation: inline

BglII
type: SequenceAnnotation
persistent identity: annotation1944497
display id: annotation1944497
version: 1
top level: U097NUL+D0
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I732408/annotation1944497/range1944497/1

https://synbiohub.org/public/igem/BBa_I732408/annotation1944497/range1944497/1
type: Range
persistent identity: range1944497
display id: range1944497
version: 1
direction: direction/forward
top level: U097NUL+D0
start: 3
end: 8
orientation: inline

START
type: SequenceAnnotation
persistent identity: annotation1944502
display id: annotation1944502
version: 1
top level: U097NUL+D0
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_I732408/annotation1944502/range1944502/1

https://synbiohub.org/public/igem/BBa_I732408/annotation1944502/range1944502/1
type: Range
persistent identity: range1944502
display id: range1944502
version: 1
direction: direction/forward
top level: U097NUL+D0
start: 46
end: 48
orientation: inline

BBa_I732007
type: SequenceAnnotation
persistent identity: annotation1944499
display id: annotation1944499
version: 1
top level: U097NUL+D0
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_I732408/annotation1944499/range1944499/1

https://synbiohub.org/public/igem/BBa_I732408/annotation1944499/range1944499/1
type: Range
persistent identity: range1944499
display id: range1944499
version: 1
direction: direction/reverse
top level: U097NUL+D0
start: 1
end: 19
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1944501
display id: annotation1944501
version: 1
top level: U097NUL+D0
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_I732408/annotation1944501/range1944501/1

https://synbiohub.org/public/igem/BBa_I732408/annotation1944501/range1944501/1
type: Range
persistent identity: range1944501
display id: range1944501
version: 1
direction: direction/reverse
top level: U097NUL+D0
start: 28
end: 39
orientation: inline

lacZ
type: SequenceAnnotation
persistent identity: annotation1944503
display id: annotation1944503
version: 1
top level: U097NUL+D0
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_I732408/annotation1944503/range1944503/1

https://synbiohub.org/public/igem/BBa_I732408/annotation1944503/range1944503/1
type: Range
persistent identity: range1944503
display id: range1944503
version: 1
direction: direction/forward
top level: U097NUL+D0
start: 46
end: 279
orientation: inline

barcode
type: SequenceAnnotation
persistent identity: annotation1944510
display id: annotation1944510
version: 1
top level: U097NUL+D0
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_I732408/annotation1944510/range1944510/1

https://synbiohub.org/public/igem/BBa_I732408/annotation1944510/range1944510/1
type: Range
persistent identity: range1944510
display id: range1944510
version: 1
direction: direction/reverse
top level: U097NUL+D0
start: 987
end: 1011
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1944507
display id: annotation1944507
version: 1
top level: U097NUL+D0
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_I732408/annotation1944507/range1944507/1

https://synbiohub.org/public/igem/BBa_I732408/annotation1944507/range1944507/1
type: Range
persistent identity: range1944507
display id: range1944507
version: 1
direction: direction/reverse
top level: U097NUL+D0
start: 288
end: 299
orientation: inline

BamHI
type: SequenceAnnotation
persistent identity: annotation1944498
display id: annotation1944498
version: 1
top level: U097NUL+D0
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I732408/annotation1944498/range1944498/1

https://synbiohub.org/public/igem/BBa_I732408/annotation1944498/range1944498/1
type: Range
persistent identity: range1944498
display id: range1944498
version: 1
direction: direction/forward
top level: U097NUL+D0
start: 12
end: 17
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1944514
display id: annotation1944514
version: 1
top level: U097NUL+D0
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_I732408/annotation1944514/range1944514/1

https://synbiohub.org/public/igem/BBa_I732408/annotation1944514/range1944514/1
type: Range
persistent identity: range1944514
display id: range1944514
version: 1
direction: direction/forward
top level: U097NUL+D0
start: 1115
end: 1134
orientation: inline

conserved
type: SequenceAnnotation
persistent identity: annotation1944506
display id: annotation1944506
version: 1
top level: U097NUL+D0
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_I732408/annotation1944506/range1944506/1

https://synbiohub.org/public/igem/BBa_I732408/annotation1944506/range1944506/1
type: Range
persistent identity: range1944506
display id: range1944506
version: 1
direction: direction/forward
top level: U097NUL+D0
start: 292
end: 295
orientation: inline

conserved
type: SequenceAnnotation
persistent identity: annotation1944500
display id: annotation1944500
version: 1
top level: U097NUL+D0
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_I732408/annotation1944500/range1944500/1

https://synbiohub.org/public/igem/BBa_I732408/annotation1944500/range1944500/1
type: Range
persistent identity: range1944500
display id: range1944500
version: 1
direction: direction/forward
top level: U097NUL+D0
start: 32
end: 35
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1944516
display id: annotation1944516
version: 1
top level: U097NUL+D0
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_I732408/annotation1944516/range1944516/1

https://synbiohub.org/public/igem/BBa_I732408/annotation1944516/range1944516/1
type: Range
persistent identity: range1944516
display id: range1944516
version: 1
direction: direction/forward
top level: U097NUL+D0
start: 1141
end: 1141
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1944511
display id: annotation1944511
version: 1
top level: U097NUL+D0
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_I732408/annotation1944511/range1944511/1

https://synbiohub.org/public/igem/BBa_I732408/annotation1944511/range1944511/1
type: Range
persistent identity: range1944511
display id: range1944511
version: 1
direction: direction/forward
top level: U097NUL+D0
start: 1020
end: 1099
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1944513
display id: annotation1944513
version: 1
top level: U097NUL+D0
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_I732408/annotation1944513/range1944513/1

https://synbiohub.org/public/igem/BBa_I732408/annotation1944513/range1944513/1
type: Range
persistent identity: range1944513
display id: range1944513
version: 1
direction: direction/forward
top level: U097NUL+D0
start: 1108
end: 1148
orientation: inline

STOP
type: SequenceAnnotation
persistent identity: annotation1944504
display id: annotation1944504
version: 1
top level: U097NUL+D0
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_I732408/annotation1944504/range1944504/1

https://synbiohub.org/public/igem/BBa_I732408/annotation1944504/range1944504/1
type: Range
persistent identity: range1944504
display id: range1944504
version: 1
direction: direction/forward
top level: U097NUL+D0
start: 274
end: 279
orientation: inline

GmR
type: ComponentDefinition
persistent identity: BBa P1012
display id: BBa_P1012
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: gentamycin/kanamycin resistance cassette
creator: Tom Knight
created: 2006-03-15T12:00:00Z
modified: 2015-05-08T01:14:12Z
discontinued: false
dominant: false
experience: experience/None
group u list: _41_48_1_
m user id: 0
owner id: 6
owning group id: 48
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: The aacA-aphD gene from the transposon Tn4001 conveys resistance to both Gentamycin and Kanamycin with a single two-domain resistance protein.
mutable notes: ;primers to make Biobrick version of GmR gene cassette (P1012) gtttcttcgaattcgcggccgcttctagagTGGACTTGACTGAGTTTATGGAA, P3aph-F gtttcttcctgcagcggccgctactagtaTCTGGACTTGACTCACTTCCTTT, P3aph-R ;P3aph-R (3' - 5') ;aaaggaagtgagtcaagtccagatactagtagcggccgctgcaggaagaaac
mutable provenance: Originally from Tn4001 transposon; sequence M18086. Created from plasmid pISM2062 from Chris Minion, Iowa State University.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: acetyl-transferase
sequence annotation: aacA-aphD (GmR, KanR)
sequence: https://synbiohub.org/public/igem/BBa_P1012_sequence/1

acetyl-transferase
type: SequenceAnnotation
persistent identity: annotation1825004
display id: annotation1825004
version: 1
top level: GmR
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_P1012/annotation1825004/range1825004/1

https://synbiohub.org/public/igem/BBa_P1012/annotation1825004/range1825004/1
type: Range
persistent identity: range1825004
display id: range1825004
version: 1
direction: direction/forward
top level: GmR
start: 81
end: 411
orientation: inline

aacA-aphD (GmR, KanR)
type: SequenceAnnotation
persistent identity: annotation1825005
display id: annotation1825005
version: 1
top level: GmR
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_P1012/annotation1825005/range1825005/1

https://synbiohub.org/public/igem/BBa_P1012/annotation1825005/range1825005/1
type: Range
persistent identity: range1825005
display id: range1825005
version: 1
direction: direction/forward
top level: GmR
start: 412
end: 1851
orientation: inline

BBa_I731004
type: ComponentDefinition
persistent identity: BBa I731004
display id: BBa_I731004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: FecA promoter
creator: Shaunak Vankudre
created: 2007-06-25T11:00:00Z
modified: 2015-08-31T04:07:55Z
discontinued: false
dominant: false
experience: experience/None
group u list: _127_
m user id: 0
owner id: 1889
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: derived from Enz et al, 2003, J Bacteriol. 2003 April; 185(7): 2387???2391. doi: 10.1128/JB.185.7.2387-2391.2003.
mutable provenance: genomic sequence from E. Coli K12
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I731004_sequence/1

BBa_J24813
type: ComponentDefinition
persistent identity: BBa J24813
display id: BBa_J24813
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: URA3 Promoter from S. cerevisiae
creator: John Szymanski
created: 2008-05-01T11:00:00Z
modified: 2015-08-31T04:08:43Z
discontinued: false
dominant: false
group u list: _58_
m user id: 0
owner id: 2512
owning group id: 58
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is the whole regulatory region from the URA3 gene coding for OMP decarboxylase in S. cerevisiae budding yeast.
mutable notes: None
mutable provenance: NCBI Sequence database: http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=Nucleotide&dopt=GenBank&val=172061
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J24813_sequence/1

BBa_I746361
type: ComponentDefinition
persistent identity: BBa I746361
display id: BBa_I746361
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PO promoter from P2 phage
creator: Stefan Milde
created: 2007-09-10T11:00:00Z
modified: 2015-08-31T04:08:04Z
discontinued: false
dominant: false
group u list: _116_
m user id: 0
owner id: 2122
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This is the PO promoter taken from the P2 phage genome. It is an inducible promoter that is activated by a class of activators, including P2 ogr (I746350), PSP3 pag (I746351) and phiR73 delta (I746352). These different activators should cause different levels of activity of the PO promoter.
mutable notes: no special considerations
mutable provenance: The source of the DNA is the P2 phage genome.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: PO
sequence: https://synbiohub.org/public/igem/BBa_I746361_sequence/1

PO
type: SequenceAnnotation
persistent identity: annotation1943784
display id: annotation1943784
version: 1
top level: BBa_I746361
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I746361/annotation1943784/range1943784/1

https://synbiohub.org/public/igem/BBa_I746361/annotation1943784/range1943784/1
type: Range
persistent identity: range1943784
display id: range1943784
version: 1
direction: direction/forward
top level: BBa_I746361
start: 1
end: 92
orientation: inline

BBa_K110007
type: ComponentDefinition
persistent identity: BBa K110007
display id: BBa_K110007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: A-Cell Promoter MFA2
creator: James DiCarlo
created: 2008-05-29T11:00:00Z
modified: 2015-05-08T01:09:09Z
discontinued: false
dominant: false
group u list: _201_
m user id: 0
owner id: 2669
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This promoter was isolated by taking 500bp upstream of the MFA2 gene of S. cerevisiae. This promoter will be activated when A factor is expressed in an A haploid yeast cell.
mutable notes: This gene is on the Watson strand of the entered sequence.
mutable provenance: SGD
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K110007_sequence/1

BBa_B1006
type: ComponentDefinition
persistent identity: BBa B1006
display id: BBa_B1006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Terminator (artificial, large, %T~>90)
creator: Haiyao Huang
created: 2006-08-30T11:00:00Z
modified: 2015-08-31T04:07:21Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _41_
m user id: 0
owner id: 745
owning group id: 41
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Artificial terminator, estimated %T~>90% *8bp stem, 6nt loop *Bidirectional, estimated reverse %T~>90%
mutable notes: Bidirectional, with the reverse estimated to be less effective than the forward. Has a polyA tail of 9 residues.
mutable provenance: modified E. coli thr terminator, replaced all A-T pairs in stem with C-G pairs
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: PolyA
sequence annotation: modified thr terminator
sequence annotation: B1006
sequence: https://synbiohub.org/public/igem/BBa_B1006_sequence/1

PolyA
type: SequenceAnnotation
persistent identity: annotation1898431
display id: annotation1898431
version: 1
top level: BBa_B1006
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_B1006/annotation1898431/range1898431/1

https://synbiohub.org/public/igem/BBa_B1006/annotation1898431/range1898431/1
type: Range
persistent identity: range1898431
display id: range1898431
version: 1
direction: direction/reverse
top level: BBa_B1006
start: 1
end: 9
orientation: inline

PolyA
type: SequenceAnnotation
persistent identity: annotation1898430
display id: annotation1898430
version: 1
top level: BBa_B1006
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_B1006/annotation1898430/range1898430/1

https://synbiohub.org/public/igem/BBa_B1006/annotation1898430/range1898430/1
type: Range
persistent identity: range1898430
display id: range1898430
version: 1
direction: direction/forward
top level: BBa_B1006
start: 32
end: 39
orientation: inline

modified thr terminator
type: SequenceAnnotation
persistent identity: annotation1898429
display id: annotation1898429
version: 1
top level: BBa_B1006
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_B1006/annotation1898429/range1898429/1

https://synbiohub.org/public/igem/BBa_B1006/annotation1898429/range1898429/1
type: Range
persistent identity: range1898429
display id: range1898429
version: 1
direction: direction/forward
top level: BBa_B1006
start: 10
end: 31
orientation: inline

B1006
type: SequenceAnnotation
persistent identity: annotation1898428
display id: annotation1898428
version: 1
top level: BBa_B1006
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_B1006/annotation1898428/range1898428/1

https://synbiohub.org/public/igem/BBa_B1006/annotation1898428/range1898428/1
type: Range
persistent identity: range1898428
display id: range1898428
version: 1
direction: direction/forward
top level: BBa_B1006
start: 1
end: 39
orientation: inline

KP3
type: ComponentDefinition
persistent identity: BBa J01006
display id: BBa_J01006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Key Promoter absorbs 3
creator: Golden Bear
created: 2005-10-31T12:00:00Z
modified: 2015-08-31T04:08:11Z
discontinued: false
dominant: false
group u list: _13_
m user id: 0
owner id: 395
owning group id: 13
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Promoter for transcribing keys to act on locks based on Isaacs, Collins, et. al: "Engineered riboregulators enable post-transcriptional control of gene expression" KP3 absorbs three nucleotides from mixed site ("TACTAGAG"), so that the key has a 5 nucleotide spacer region (i.e. "TAGAG") between the transcription start site and the first nucleotide of the key. We are still looking into what the optimal size for the spacer region is. See also KP2 (BBa_J01007), and Key1 (BBa_J01008), Key2 (BBa_J01009), Lock1 (BBa_J01010), and Lock2 (BBa_J01011).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J01006_sequence/1

BBa_J23100
type: ComponentDefinition
persistent identity: BBa J23100
display id: BBa_J23100
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: constitutive promoter family member
creator: John Anderson
created: 2006-08-03T11:00:00Z
modified: 2015-08-31T04:08:40Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: Replace later
mutable notes: N/A
mutable provenance: Isolated from library of promoters
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J23100_sequence/1

BBa_K1315000
type: ComponentDefinition
persistent identity: BBa K1315000
display id: BBa_K1315000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pqsR
creator: Dimitrios Michailidis
created: 2014-09-19T11:00:00Z
modified: 2015-05-08T01:09:48Z
discontinued: true
dominant: false
experience: experience/None
group u list: _1690_
m user id: 0
owner id: 21812
owning group id: 9
sample status: Discontinued
status: status/Deleted
bookmark: false
mutable description: The Pseydomonas quinolone signal (PQS) as well as its precursor 2-heptyl-4-quinolone (HHQ)are very important in mechanisms controlling virulence in Pseudomonas aeruginosa. P. aeruginosa has a key role in Cystic Fibrosis (CF). PQS can initiate signalling for various processes like virulence, motility, and biofilm formation. A good number of these processes are facilitated by PqsR, which is a transcriptional factor. An activated PqsR will go to the nucleus of the cells and activate the respective genes. http://www.ncbi.nlm.nih.gov/pubmed/21539583 http://www.ncbi.nlm.nih.gov/pubmed/?term=pqsr-dependent+and+pqsr-independent+regulation+of+motility+and+biofilm+formation+by+pqs http://www.ncbi.nlm.nih.gov/pubmed/12393198
mutable notes: The sequence did not have any restriction sites to remove so it was immediately PCRed out of the genome and had the registry prefix and suffix added to it. It was then ligated into the pSB1C3 vector.
mutable provenance: The source of this part is the genome of Pseudomonas aeruginosa PAO1 provided by the Robert Ryan lab in the Molecular Microbiology department of the University of Dundee, UK.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: PqsR
sequence: https://synbiohub.org/public/igem/BBa_K1315000_sequence/1

PqsR
type: SequenceAnnotation
persistent identity: annotation2384759
display id: annotation2384759
version: 1
top level: BBa_K1315000
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1315000/annotation2384759/range2384759/1

https://synbiohub.org/public/igem/BBa_K1315000/annotation2384759/range2384759/1
type: Range
persistent identity: range2384759
display id: range2384759
version: 1
direction: direction/forward
top level: BBa_K1315000
start: 1
end: 999
orientation: inline

BBa_J61102
type: ComponentDefinition
persistent identity: BBa J61102
display id: BBa_J61102
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Ribosome Binding Site Family Member
creator: John Anderson
created: 2007-01-28T12:00:00Z
modified: 2015-08-31T02:03:00Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: false
mutable description: {{JCA_Arkin_RBSFamily}}
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J61102_sequence/1

pJEJAM16
type: ComponentDefinition
persistent identity: BBa K678064
display id: BBa_K678064
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pJEJAM16
creator: DTU-Denmark-2
created: 2011-09-12T11:00:00Z
modified: 2015-05-08T01:13:05Z
discontinued: false
dominant: false
experience: experience/None
group u list: _882_
m user id: 0
owner id: 8341
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: To be announced
mutable notes: To be announced
mutable provenance: To be announced
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000155
role: partType/Plasmid
component: BBa_K678046
component: BBa_K678036
component: BBa_K678024
component: BBa_K678029
component: BBa_K678040
component: BBa_K678025
sequence annotation: BBa_K678040
sequence annotation: BBa_K678024
sequence annotation: BBa_K678029
sequence annotation: BBa_K678025
sequence annotation: BBa_K678036
sequence annotation: BBa_K678046
sequence: https://synbiohub.org/public/igem/BBa_K678064_sequence/1

BBa_K678046
type: Component
persistent identity: component2127569
display id: component2127569
version: 1
top level: pJEJAM16
access: public
definition: pFun

BBa_K678036
type: Component
persistent identity: component2127563
display id: component2127563
version: 1
top level: pJEJAM16
access: public
definition: TtrpC

BBa_K678024
type: Component
persistent identity: component2127554
display id: component2127554
version: 1
top level: pJEJAM16
access: public
definition: PgpdA

BBa_K678029
type: Component
persistent identity: component2127560
display id: component2127560
version: 1
top level: pJEJAM16
access: public
definition: GFP-TS

BBa_K678040
type: Component
persistent identity: component2127566
display id: component2127566
version: 1
top level: pJEJAM16
access: public
definition: pyrG casse

BBa_K678025
type: Component
persistent identity: component2127557
display id: component2127557
version: 1
top level: pJEJAM16
access: public
definition: MTS

BBa_K678040
type: SequenceAnnotation
persistent identity: annotation2127566
display id: annotation2127566
version: 1
top level: pJEJAM16
location: https://synbiohub.org/public/igem/BBa_K678064/annotation2127566/range2127566/1
component: BBa_K678040

https://synbiohub.org/public/igem/BBa_K678064/annotation2127566/range2127566/1
type: Range
persistent identity: range2127566
display id: range2127566
version: 1
direction: direction/reverse
top level: pJEJAM16
start: 4021
end: 5476
orientation: inline

BBa_K678024
type: SequenceAnnotation
persistent identity: annotation2127554
display id: annotation2127554
version: 1
top level: pJEJAM16
location: https://synbiohub.org/public/igem/BBa_K678064/annotation2127554/range2127554/1
component: BBa_K678024

https://synbiohub.org/public/igem/BBa_K678064/annotation2127554/range2127554/1
type: Range
persistent identity: range2127554
display id: range2127554
version: 1
direction: direction/reverse
top level: pJEJAM16
start: 1
end: 2312
orientation: inline

BBa_K678029
type: SequenceAnnotation
persistent identity: annotation2127560
display id: annotation2127560
version: 1
top level: pJEJAM16
location: https://synbiohub.org/public/igem/BBa_K678064/annotation2127560/range2127560/1
component: BBa_K678029

https://synbiohub.org/public/igem/BBa_K678064/annotation2127560/range2127560/1
type: Range
persistent identity: range2127560
display id: range2127560
version: 1
direction: direction/reverse
top level: pJEJAM16
start: 2517
end: 3245
orientation: inline

BBa_K678025
type: SequenceAnnotation
persistent identity: annotation2127557
display id: annotation2127557
version: 1
top level: pJEJAM16
location: https://synbiohub.org/public/igem/BBa_K678064/annotation2127557/range2127557/1
component: BBa_K678025

https://synbiohub.org/public/igem/BBa_K678064/annotation2127557/range2127557/1
type: Range
persistent identity: range2127557
display id: range2127557
version: 1
direction: direction/reverse
top level: pJEJAM16
start: 2321
end: 2508
orientation: inline

BBa_K678036
type: SequenceAnnotation
persistent identity: annotation2127563
display id: annotation2127563
version: 1
top level: pJEJAM16
location: https://synbiohub.org/public/igem/BBa_K678064/annotation2127563/range2127563/1
component: BBa_K678036

https://synbiohub.org/public/igem/BBa_K678064/annotation2127563/range2127563/1
type: Range
persistent identity: range2127563
display id: range2127563
version: 1
direction: direction/reverse
top level: pJEJAM16
start: 3254
end: 4012
orientation: inline

BBa_K678046
type: SequenceAnnotation
persistent identity: annotation2127569
display id: annotation2127569
version: 1
top level: pJEJAM16
location: https://synbiohub.org/public/igem/BBa_K678064/annotation2127569/range2127569/1
component: BBa_K678046

https://synbiohub.org/public/igem/BBa_K678064/annotation2127569/range2127569/1
type: Range
persistent identity: range2127569
display id: range2127569
version: 1
direction: direction/reverse
top level: pJEJAM16
start: 5485
end: 8362
orientation: inline

BBa_K136002
type: ComponentDefinition
persistent identity: BBa K136002
display id: BBa_K136002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter of flgB gene from E. coli flagella
creator: Cyprien Maisonnier
created: 2008-08-11T11:00:00Z
modified: 2015-05-08T01:10:02Z
discontinued: true
dominant: false
experience: experience/None
group u list: _211_
m user id: 0
owner id: 3012
owning group id: 9
sample status: Discontinued
status: status/Deleted
bookmark: false
mutable description: ''flgB'' is a class 2 gene that is part of the construction of the flagella ( ''Ordering Genes in a Flagella Pathway by Analysis of Expression Kinetics from Living Bacteria'' - S. Kalir, J. McClure, K. Pabbaraju, C. Southward, M. Ronen, S. Leibler, M. G. Surette, U. Alon ). Its promoter is consitutively repressed. It is activated by FlhD4C2 and maybe by FliA with different strength ( ''Using a Quantitative Blueprint to Reprogram the Dynamics of the Flagella Gene Network'' - Shiraz Kalir and Uri Alon ). '''Work in progress :''' Deeper quantitative characterization
mutable notes: The primers used to amplify this sequence are : *Forward : GTTTCTTCGAATTCGCGGCCGCTTCTAGAGACAGTATCGCGATGATCGCCACGCTACG *Reverse : GTTTCTTCCTGCAGCGGCCGCTACTAGTAAGCATATCTCCTCCGCAGGTATCAAAATT
mutable provenance: This part comes from the genome of ''E.coli'' K12 strain MG1655.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: Initiation of Transcription
sequence annotation: FlhDC Binding site 1
sequence annotation: FlhDC Binding site 2
sequence: https://synbiohub.org/public/igem/BBa_K136002_sequence/1

Initiation of Transcription
type: SequenceAnnotation
persistent identity: annotation1971573
display id: annotation1971573
version: 1
top level: BBa_K136002
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K136002/annotation1971573/range1971573/1

https://synbiohub.org/public/igem/BBa_K136002/annotation1971573/range1971573/1
type: Range
persistent identity: range1971573
display id: range1971573
version: 1
direction: direction/forward
top level: BBa_K136002
start: 173
end: 173
orientation: inline

FlhDC Binding site 1
type: SequenceAnnotation
persistent identity: annotation1971571
display id: annotation1971571
version: 1
top level: BBa_K136002
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K136002/annotation1971571/range1971571/1

https://synbiohub.org/public/igem/BBa_K136002/annotation1971571/range1971571/1
type: Range
persistent identity: range1971571
display id: range1971571
version: 1
direction: direction/forward
top level: BBa_K136002
start: 98
end: 113
orientation: inline

FlhDC Binding site 2
type: SequenceAnnotation
persistent identity: annotation1971572
display id: annotation1971572
version: 1
top level: BBa_K136002
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K136002/annotation1971572/range1971572/1

https://synbiohub.org/public/igem/BBa_K136002/annotation1971572/range1971572/1
type: Range
persistent identity: range1971572
display id: range1971572
version: 1
direction: direction/forward
top level: BBa_K136002
start: 124
end: 139
orientation: inline

BBa_J61125
type: ComponentDefinition
persistent identity: BBa J61125
display id: BBa_J61125
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Ribosome Binding Site Family Member
creator: John Anderson
created: 2007-04-24T11:00:00Z
modified: 2015-08-31T01:59:49Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: false
mutable description: {{JCA_Arkin_RBSFamily}}
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J61125_sequence/1

BBa_K1486001
type: ComponentDefinition
persistent identity: BBa K1486001
display id: BBa_K1486001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Arabinose promoter + CpxR
creator: iGem EPFL 2014
created: 2014-08-11T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: The part contains CpxR with an upstream arabinose-inducible promoter.
mutable notes: The arabinose had to be upstream of the CpxR. Also, the sequence had to be tested because of a mutation, and it works perfectly.
mutable provenance: The CpxR comes from the genome of E coli, and the arabinose promoter comes from a biobrick.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_I0500
component: BBa_K1486000
component: BBa_B0034
sequence annotation: BBa_K1486000
sequence annotation: BBa_B0034
sequence annotation: BBa_I0500
sequence: https://synbiohub.org/public/igem/BBa_K1486001_sequence/1

BBa_I0500
type: Component
persistent identity: component2382072
display id: component2382072
version: 1
top level: BBa_K1486001
access: public
definition: pBad/araC

BBa_K1486000
type: Component
persistent identity: component2382078
display id: component2382078
version: 1
top level: BBa_K1486001
access: public
definition: BBa_K1486000

BBa_B0034
type: Component
persistent identity: component2382074
display id: component2382074
version: 1
top level: BBa_K1486001
access: public
definition: BBa_B0034

BBa_K1486000
type: SequenceAnnotation
persistent identity: annotation2382078
display id: annotation2382078
version: 1
top level: BBa_K1486001
location: https://synbiohub.org/public/igem/BBa_K1486001/annotation2382078/range2382078/1
component: BBa_K1486000

https://synbiohub.org/public/igem/BBa_K1486001/annotation2382078/range2382078/1
type: Range
persistent identity: range2382078
display id: range2382078
version: 1
direction: direction/reverse
top level: BBa_K1486001
start: 1223
end: 1924
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2382074
display id: annotation2382074
version: 1
top level: BBa_K1486001
location: https://synbiohub.org/public/igem/BBa_K1486001/annotation2382074/range2382074/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1486001/annotation2382074/range2382074/1
type: Range
persistent identity: range2382074
display id: range2382074
version: 1
direction: direction/reverse
top level: BBa_K1486001
start: 1211
end: 1222
orientation: inline

BBa_I0500
type: SequenceAnnotation
persistent identity: annotation2382072
display id: annotation2382072
version: 1
top level: BBa_K1486001
location: https://synbiohub.org/public/igem/BBa_K1486001/annotation2382072/range2382072/1
component: BBa_I0500

https://synbiohub.org/public/igem/BBa_K1486001/annotation2382072/range2382072/1
type: Range
persistent identity: range2382072
display id: range2382072
version: 1
direction: direction/reverse
top level: BBa_K1486001
start: 1
end: 1210
orientation: inline

BBa_I718018
type: ComponentDefinition
persistent identity: BBa I718018
display id: BBa_I718018
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: dapAp promoter
creator: Eimad Shotar
created: 2007-10-25T11:00:00Z
modified: 2015-08-31T04:07:53Z
discontinued: false
dominant: false
group u list: _141_
m user id: 0
owner id: 1568
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: dapAp is the promter of dapA gene in E.coli. DapA enzyme is part of diaminopimelate (DAP)biosynthesis pathway. DAP is necessary for peptidoglycan and lysine biosynthesis. dapAp prmoter has been previously studied: Acord04: Acord J, Masters M (2004). "Expression from the Escherichia coli dapA promoter is regulated by intracellular levels of diaminopimelic acid." FEMS Microbiol Lett 235(1);131-7. PMID: 15158272 It has been shown that dapAp driven expression is inhibited at hight DAP. We have tried to confirm this by
mutable notes: None
mutable provenance: none yet
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I718018_sequence/1

BBa_K259007
type: ComponentDefinition
persistent identity: BBa K259007
display id: BBa_K259007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: AraC Promoter fused with RBS
creator: Petros Mina
created: 2009-09-08T11:00:00Z
modified: 2015-05-08T01:11:42Z
discontinued: false
dominant: false
group u list: _357_
m user id: 0
owner id: 5318
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part has the arabinose regulated promoter (AraC/pBAD) upstream of a ribosome binding site. This is ideal for inserting a protein to be expressed downstream of this part as it's expression can be regulated by the presence of the concentration of arabinose in the growing environment/media of the cells. ==What this part actually does== This composite part includes the AraC/pBAD promoter and a strong ribosome binding site designed from the Anderson family of RBS. The AraC/pBAD promoter can be used with prokaryotic chassis to regulate expresion of proteins. When the AraC/pBAD promoter is placed upstream of your canditate translational unit (protein to be expressed) it allows control of the trancription of the said protein. This is done via controlling the concentration of arabinose sugar in the environment/solution of bacterial growth. Controlling the transcription will not ensure translation. The ribosome binding site is ideally placed a small distance away from the promoter site to ensure that the ribosome will bind and start translating the protein coding sequence found downstream.
mutable notes: The part was designed with RFC10 assembly from supplied biobricks of the iGEM2009 distribution.
mutable provenance: The AraC promoter can be found naturally at 1.52min of the E.coli genome ([http://ecogene.org/geneinfo.php?eg_id=EG10054 EcoGene number EG10054]. The RBS was taken from the designed sequences of RBS by the Anderson family. For more information visit the original part page [http://partsregistry.org/Part:BBa_J61100 here].
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
component: BBa_R0081
component: BBa_R0080
component: BBa_J61100
sequence annotation: BBa_R0080
sequence annotation: BBa_R0081
sequence annotation: BBa_J61100
sequence: https://synbiohub.org/public/igem/BBa_K259007_sequence/1

BBa_R0081
type: Component
persistent identity: component2020539
display id: component2020539
version: 1
top level: BBa_K259007
access: public
definition: https://synbiohub.org/public/igem/BBa_R0081/1

BBa_R0080
type: Component
persistent identity: component2020546
display id: component2020546
version: 1
top level: BBa_K259007
access: public
definition: https://synbiohub.org/public/igem/BBa_R0080/1

BBa_J61100
type: Component
persistent identity: component2020547
display id: component2020547
version: 1
top level: BBa_K259007
access: public
definition: BBa_J61100

BBa_R0080
type: SequenceAnnotation
persistent identity: annotation2020546
display id: annotation2020546
version: 1
top level: BBa_K259007
location: https://synbiohub.org/public/igem/BBa_K259007/annotation2020546/range2020546/1
component: BBa_R0080

https://synbiohub.org/public/igem/BBa_K259007/annotation2020546/range2020546/1
type: Range
persistent identity: range2020546
display id: range2020546
version: 1
direction: direction/reverse
top level: BBa_K259007
start: 192
end: 340
orientation: inline

BBa_R0081
type: SequenceAnnotation
persistent identity: annotation2020539
display id: annotation2020539
version: 1
top level: BBa_K259007
location: https://synbiohub.org/public/igem/BBa_K259007/annotation2020539/range2020539/1
component: BBa_R0081

https://synbiohub.org/public/igem/BBa_K259007/annotation2020539/range2020539/1
type: Range
persistent identity: range2020539
display id: range2020539
version: 1
direction: direction/reverse
top level: BBa_K259007
start: 1
end: 183
orientation: inline

BBa_J61100
type: SequenceAnnotation
persistent identity: annotation2020547
display id: annotation2020547
version: 1
top level: BBa_K259007
location: https://synbiohub.org/public/igem/BBa_K259007/annotation2020547/range2020547/1
component: BBa_J61100

https://synbiohub.org/public/igem/BBa_K259007/annotation2020547/range2020547/1
type: Range
persistent identity: range2020547
display id: range2020547
version: 1
direction: direction/reverse
top level: BBa_K259007
start: 349
end: 360
orientation: inline

Pxyl
type: ComponentDefinition
persistent identity: BBa K143014
display id: BBa_K143014
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Xyl for B.subtilis
creator: James Chappell
created: 2008-09-14T11:00:00Z
modified: 2015-05-08T01:10:23Z
discontinued: false
dominant: false
experience: experience/None
group u list: _199_
m user id: 0
owner id: 2090
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Promoter Xylose is an inducible promoter that has been designed for high expression in ''B.subtilis''. Gene expression under the promoter xylose can be induced by addition of xylose. The context with which we used the promoter xylose, was to take an input of xylose and give '''Polymerase Per Second'''(PoPS) as an output. Promoter xylose is an inducible promoter that has been designed for high expression in ''B.subtilis''. Gene expression under the promoter xylose can be induced by addition of xylose. The context with which we used the promoter xylose, was to take an input of xylose and give '''Polymerase Per Second'''(PoPS) as an output. Xylose does not induce the promoter xylose directly, but requires the transcriptional regulator '''XylR''', ( BBa_K143036 ) This means that XylR must be constitutively expressed in ''B.subtilis'' in order to use the promoter xylose.
mutable notes: Biobrick standard was applied to the promoter xylose sequence.
mutable provenance: The part was designed using the sequence from the ''B.subtilis'' genome and from previously published papers 12. This sequence was then synthesised by Geneart.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: Sigma A -35
sequence annotation: Sigma A -10
sequence annotation: XylR Operator
sequence: https://synbiohub.org/public/igem/BBa_K143014_sequence/1

Sigma A -35
type: SequenceAnnotation
persistent identity: annotation1976428
display id: annotation1976428
version: 1
top level: Pxyl
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K143014/annotation1976428/range1976428/1

https://synbiohub.org/public/igem/BBa_K143014/annotation1976428/range1976428/1
type: Range
persistent identity: range1976428
display id: range1976428
version: 1
direction: direction/forward
top level: Pxyl
start: 13
end: 18
orientation: inline

Sigma A -10
type: SequenceAnnotation
persistent identity: annotation1976429
display id: annotation1976429
version: 1
top level: Pxyl
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K143014/annotation1976429/range1976429/1

https://synbiohub.org/public/igem/BBa_K143014/annotation1976429/range1976429/1
type: Range
persistent identity: range1976429
display id: range1976429
version: 1
direction: direction/forward
top level: Pxyl
start: 36
end: 41
orientation: inline

XylR Operator
type: SequenceAnnotation
persistent identity: annotation1975868
display id: annotation1975868
version: 1
top level: Pxyl
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K143014/annotation1975868/range1975868/1

https://synbiohub.org/public/igem/BBa_K143014/annotation1975868/range1975868/1
type: Range
persistent identity: range1975868
display id: range1975868
version: 1
direction: direction/forward
top level: Pxyl
start: 51
end: 61
orientation: inline

XylR Operator
type: SequenceAnnotation
persistent identity: annotation1975869
display id: annotation1975869
version: 1
top level: Pxyl
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K143014/annotation1975869/range1975869/1

https://synbiohub.org/public/igem/BBa_K143014/annotation1975869/range1975869/1
type: Range
persistent identity: range1975869
display id: range1975869
version: 1
direction: direction/forward
top level: Pxyl
start: 65
end: 75
orientation: inline

BBa_J61130
type: ComponentDefinition
persistent identity: BBa J61130
display id: BBa_J61130
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Ribosome Binding Site Family Member
creator: John Anderson
created: 2007-04-24T11:00:00Z
modified: 2015-08-31T01:59:49Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: false
mutable description: {{JCA_Arkin_RBSFamily}}
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J61130_sequence/1

cI HK022
type: ComponentDefinition
persistent identity: BBa R0050
display id: BBa_R0050
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter (HK022 cI regulated)
creator: Reshma Shetty
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: The pR regulatory region is a 98 base-pair sequence with standard BioBrick prefix and suffix sections on its ends. It contains two operator sites at which HK022 cI protein can bind cooperatively. The -35 and -10 regions from pR are included along with some flanking sequences. The cutoff for the 5' end of the regulatory region was placed just after the pM -10 region which promotes transcription of a gene upstream (thus the pM -10 region is not included). The cutoff for the 3' end of the regulatory region was placed immediately prior to the RBS.
mutable notes: Derived from STHK022N . Literature claims that there is no cross-talk between HK022 cI regulatory region, Lambda cI, 434 cI and P22 cI, but this has not been verified experimentally by the synthetic biology group at MIT.
mutable provenance: Bacteriophage HK022.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -35
sequence annotation: OR1
sequence annotation: BBa_R0050
sequence annotation: -10
sequence annotation: OR2
sequence annotation: putative
sequence: https://synbiohub.org/public/igem/BBa_R0050_sequence/1

-35
type: SequenceAnnotation
persistent identity: annotation2012
display id: annotation2012
version: 1
top level: cI HK022
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0050/annotation2012/range2012/1

https://synbiohub.org/public/igem/BBa_R0050/annotation2012/range2012/1
type: Range
persistent identity: range2012
display id: range2012
version: 1
direction: direction/forward
top level: cI HK022
start: 8
end: 13
orientation: inline

OR1
type: SequenceAnnotation
persistent identity: annotation2015
display id: annotation2015
version: 1
top level: cI HK022
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_R0050/annotation2015/range2015/1

https://synbiohub.org/public/igem/BBa_R0050/annotation2015/range2015/1
type: Range
persistent identity: range2015
display id: range2015
version: 1
direction: direction/forward
top level: cI HK022
start: 37
end: 51
orientation: inline

BBa_R0050
type: SequenceAnnotation
persistent identity: annotation7066
display id: annotation7066
version: 1
top level: cI HK022
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_R0050/annotation7066/range7066/1

https://synbiohub.org/public/igem/BBa_R0050/annotation7066/range7066/1
type: Range
persistent identity: range7066
display id: range7066
version: 1
direction: direction/forward
top level: cI HK022
start: 1
end: 55
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2014
display id: annotation2014
version: 1
top level: cI HK022
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0050/annotation2014/range2014/1

https://synbiohub.org/public/igem/BBa_R0050/annotation2014/range2014/1
type: Range
persistent identity: range2014
display id: range2014
version: 1
direction: direction/forward
top level: cI HK022
start: 30
end: 35
orientation: inline

OR2
type: SequenceAnnotation
persistent identity: annotation2013
display id: annotation2013
version: 1
top level: cI HK022
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_R0050/annotation2013/range2013/1

https://synbiohub.org/public/igem/BBa_R0050/annotation2013/range2013/1
type: Range
persistent identity: range2013
display id: range2013
version: 1
direction: direction/forward
top level: cI HK022
start: 13
end: 27
orientation: inline

putative
type: SequenceAnnotation
persistent identity: annotation2018
display id: annotation2018
version: 1
top level: cI HK022
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_R0050/annotation2018/range2018/1

https://synbiohub.org/public/igem/BBa_R0050/annotation2018/range2018/1
type: Range
persistent identity: range2018
display id: range2018
version: 1
direction: direction/forward
top level: cI HK022
start: 43
end: 43
orientation: inline

BBa_J61136
type: ComponentDefinition
persistent identity: BBa J61136
display id: BBa_J61136
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Ribosome Binding Site Family Member
creator: John Anderson
created: 2007-04-24T11:00:00Z
modified: 2015-08-31T01:59:49Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: false
mutable description: {{JCA_Arkin_RBSFamily}}
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J61136_sequence/1

BBa_B0032
type: ComponentDefinition
persistent identity: BBa B0032
display id: BBa_B0032
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS.3 (medium) -- derivative of BBa_0030
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _41_44_48_46_1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Weak1 RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0033</bb_part>.
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("RBS-2" in figure 4-14 of thesis). Contact info for this part: Brian Chow
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: BBa_B0032
sequence annotation: RBS
sequence annotation: RBS-3\Weak
sequence: https://synbiohub.org/public/igem/BBa_B0032_sequence/1

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation7027
display id: annotation7027
version: 1
top level: BBa_B0032
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_B0032/annotation7027/range7027/1

https://synbiohub.org/public/igem/BBa_B0032/annotation7027/range7027/1
type: Range
persistent identity: range7027
display id: range7027
version: 1
direction: direction/forward
top level: BBa_B0032
start: 1
end: 13
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation1710
display id: annotation1710
version: 1
top level: BBa_B0032
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_B0032/annotation1710/range1710/1

https://synbiohub.org/public/igem/BBa_B0032/annotation1710/range1710/1
type: Range
persistent identity: range1710
display id: range1710
version: 1
direction: direction/forward
top level: BBa_B0032
start: 7
end: 10
orientation: inline

RBS-3\Weak
type: SequenceAnnotation
persistent identity: annotation1709
display id: annotation1709
version: 1
top level: BBa_B0032
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_B0032/annotation1709/range1709/1

https://synbiohub.org/public/igem/BBa_B0032/annotation1709/range1709/1
type: Range
persistent identity: range1709
display id: range1709
version: 1
direction: direction/forward
top level: BBa_B0032
start: 1
end: 13
orientation: inline

BBa_R2109
type: ComponentDefinition
persistent identity: BBa R2109
display id: BBa_R2109
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter with operator site for C2003
creator: Reshma Shetty
created: 2006-10-20T11:00:00Z
modified: 2015-05-08T01:14:16Z
discontinued: false
dominant: false
group u list: _41_
m user id: 0
owner id: 126
owning group id: 70
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This promoter is designed to bind to C2003.
mutable notes: Placement of the operator site was determined to optimize steric interference between C2003 and RNA polymerase.
mutable provenance: This promoter was rationally designed and synthesized.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -10 site
sequence annotation: -35 site
sequence annotation: transcription start site
sequence annotation: operator site for C2003
sequence: https://synbiohub.org/public/igem/BBa_R2109_sequence/1

-10 site
type: SequenceAnnotation
persistent identity: annotation1903913
display id: annotation1903913
version: 1
top level: BBa_R2109
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R2109/annotation1903913/range1903913/1

https://synbiohub.org/public/igem/BBa_R2109/annotation1903913/range1903913/1
type: Range
persistent identity: range1903913
display id: range1903913
version: 1
direction: direction/forward
top level: BBa_R2109
start: 40
end: 45
orientation: inline

-35 site
type: SequenceAnnotation
persistent identity: annotation1903912
display id: annotation1903912
version: 1
top level: BBa_R2109
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R2109/annotation1903912/range1903912/1

https://synbiohub.org/public/igem/BBa_R2109/annotation1903912/range1903912/1
type: Range
persistent identity: range1903912
display id: range1903912
version: 1
direction: direction/forward
top level: BBa_R2109
start: 17
end: 22
orientation: inline

transcription start site
type: SequenceAnnotation
persistent identity: annotation1903914
display id: annotation1903914
version: 1
top level: BBa_R2109
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_R2109/annotation1903914/range1903914/1

https://synbiohub.org/public/igem/BBa_R2109/annotation1903914/range1903914/1
type: Range
persistent identity: range1903914
display id: range1903914
version: 1
direction: direction/forward
top level: BBa_R2109
start: 53
end: 53
orientation: inline

operator site for C2003
type: SequenceAnnotation
persistent identity: annotation1903915
display id: annotation1903915
version: 1
top level: BBa_R2109
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_R2109/annotation1903915/range1903915/1

https://synbiohub.org/public/igem/BBa_R2109/annotation1903915/range1903915/1
type: Range
persistent identity: range1903915
display id: range1903915
version: 1
direction: direction/forward
top level: BBa_R2109
start: 22
end: 33
orientation: inline

BBa_K094002
type: ComponentDefinition
persistent identity: BBa K094002
display id: BBa_K094002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: plambda P(O-R12)
creator: Shi Lei
created: 2008-07-01T11:00:00Z
modified: 2015-05-08T01:08:40Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _255_
m user id: 0
owner id: 2053
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: just for testing
mutable notes: just for testing
mutable provenance: just for testing
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: promoter
sequence: https://synbiohub.org/public/igem/BBa_K094002_sequence/1

promoter
type: SequenceAnnotation
persistent identity: annotation1985278
display id: annotation1985278
version: 1
top level: BBa_K094002
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K094002/annotation1985278/range1985278/1

https://synbiohub.org/public/igem/BBa_K094002/annotation1985278/range1985278/1
type: Range
persistent identity: range1985278
display id: range1985278
version: 1
direction: direction/forward
top level: BBa_K094002
start: 1
end: 100
orientation: inline

BBa_K678005
type: ComponentDefinition
persistent identity: BBa K678005
display id: BBa_K678005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: SV40, mammalian promoter
creator: DTU-Denmark-2
created: 2011-09-03T11:00:00Z
modified: 2015-05-08T01:13:04Z
discontinued: false
dominant: false
experience: experience/None
group u list: _882_
m user id: 0
owner id: 8341
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Promoter for expression in mammalian cells. The SV40 promoter contains an ori.
mutable notes: Compatible with the Plug'n'Play assembly standard
mutable provenance: Plasmid pcDNA3.1 from Invitrogen
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: SV40 + ori
sequence annotation: Linker 1
sequence: https://synbiohub.org/public/igem/BBa_K678005_sequence/1

SV40 + ori
type: SequenceAnnotation
persistent identity: annotation2127346
display id: annotation2127346
version: 1
top level: BBa_K678005
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K678005/annotation2127346/range2127346/1

https://synbiohub.org/public/igem/BBa_K678005/annotation2127346/range2127346/1
type: Range
persistent identity: range2127346
display id: range2127346
version: 1
direction: direction/forward
top level: BBa_K678005
start: 9
end: 352
orientation: inline

Linker 1
type: SequenceAnnotation
persistent identity: annotation2127347
display id: annotation2127347
version: 1
top level: BBa_K678005
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K678005/annotation2127347/range2127347/1

https://synbiohub.org/public/igem/BBa_K678005/annotation2127347/range2127347/1
type: Range
persistent identity: range2127347
display id: range2127347
version: 1
direction: direction/forward
top level: BBa_K678005
start: 1
end: 8
orientation: inline

BBa_K1486022
type: ComponentDefinition
persistent identity: BBa K1486022
display id: BBa_K1486022
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Renilla Luciferase
creator: EPFL iGem team 2014
created: 2014-09-14T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This part is the full renilla luciferase. The split protein has also been submitted as biobricks: BBa_K1486021.
mutable notes: to edit
mutable provenance: To edit
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0034
component: BBa_K1486019
component: BBa_K1486020
component: BBa_I0500
sequence annotation: BBa_I0500
sequence annotation: BBa_B0034
sequence annotation: BBa_K1486020
sequence annotation: BBa_K1486019
sequence: https://synbiohub.org/public/igem/BBa_K1486022_sequence/1

BBa_B0034
type: Component
persistent identity: component2383473
display id: component2383473
version: 1
top level: BBa_K1486022
access: public
definition: BBa_B0034

BBa_K1486019
type: Component
persistent identity: component2383474
display id: component2383474
version: 1
top level: BBa_K1486022
access: public
definition: BBa_K1486019

BBa_K1486020
type: Component
persistent identity: component2383475
display id: component2383475
version: 1
top level: BBa_K1486022
access: public
definition: BBa_K1486020

BBa_I0500
type: Component
persistent identity: component2383471
display id: component2383471
version: 1
top level: BBa_K1486022
access: public
definition: pBad/araC

BBa_I0500
type: SequenceAnnotation
persistent identity: annotation2383471
display id: annotation2383471
version: 1
top level: BBa_K1486022
location: https://synbiohub.org/public/igem/BBa_K1486022/annotation2383471/range2383471/1
component: BBa_I0500

https://synbiohub.org/public/igem/BBa_K1486022/annotation2383471/range2383471/1
type: Range
persistent identity: range2383471
display id: range2383471
version: 1
direction: direction/reverse
top level: BBa_K1486022
start: 1
end: 1210
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2383473
display id: annotation2383473
version: 1
top level: BBa_K1486022
location: https://synbiohub.org/public/igem/BBa_K1486022/annotation2383473/range2383473/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1486022/annotation2383473/range2383473/1
type: Range
persistent identity: range2383473
display id: range2383473
version: 1
direction: direction/reverse
top level: BBa_K1486022
start: 1211
end: 1222
orientation: inline

BBa_K1486020
type: SequenceAnnotation
persistent identity: annotation2383475
display id: annotation2383475
version: 1
top level: BBa_K1486022
location: https://synbiohub.org/public/igem/BBa_K1486022/annotation2383475/range2383475/1
component: BBa_K1486020

https://synbiohub.org/public/igem/BBa_K1486022/annotation2383475/range2383475/1
type: Range
persistent identity: range2383475
display id: range2383475
version: 1
direction: direction/reverse
top level: BBa_K1486022
start: 1553
end: 2158
orientation: inline

BBa_K1486019
type: SequenceAnnotation
persistent identity: annotation2383474
display id: annotation2383474
version: 1
top level: BBa_K1486022
location: https://synbiohub.org/public/igem/BBa_K1486022/annotation2383474/range2383474/1
component: BBa_K1486019

https://synbiohub.org/public/igem/BBa_K1486022/annotation2383474/range2383474/1
type: Range
persistent identity: range2383474
display id: range2383474
version: 1
direction: direction/reverse
top level: BBa_K1486022
start: 1223
end: 1552
orientation: inline

BBa_M13504
type: ComponentDefinition
persistent identity: BBa M13504
display id: BBa_M13504
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: M13K07 gene IV RBS
creator: Natalie Kuldell
created: 2006-12-14T12:00:00Z
modified: 2015-05-08T01:13:56Z
discontinued: false
dominant: false
experience: experience/None
group u list: _45_
m user id: 0
owner id: 314
owning group id: 1
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This RBS is part of the bacteriophage M13 genome, initiating translation of the gene IV message (BBa_M13004). The part aligns with base pairs 4204-4219 in M13K07 genome. It was identified as the RBS for gene IV message by Wezenbeek et al in Gene (1980) 11: 129-148
mutable notes: none
mutable provenance: New England Biolabs M13K07. Sequenced in 2002 by L. Panganaban
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_M13504_sequence/1

BBa_I739104
type: ComponentDefinition
persistent identity: BBa I739104
display id: BBa_I739104
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Double Promoter (LuxR/HSL, positive / P22 cII, negative)
creator: Stefan Luzi
created: 2007-10-14T11:00:00Z
modified: 2015-08-31T04:08:01Z
discontinued: false
dominant: false
group u list: _124_
m user id: 0
owner id: 2183
owning group id: 62
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: asdf
mutable notes: asdf
mutable provenance: Synthetic DNA
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: R0053 (2x OR1)
sequence annotation: Scar
sequence annotation: OR1
sequence annotation: R0062
sequence: https://synbiohub.org/public/igem/BBa_I739104_sequence/1

R0053 (2x OR1)
type: SequenceAnnotation
persistent identity: annotation1947895
display id: annotation1947895
version: 1
top level: BBa_I739104
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_I739104/annotation1947895/range1947895/1

https://synbiohub.org/public/igem/BBa_I739104/annotation1947895/range1947895/1
type: Range
persistent identity: range1947895
display id: range1947895
version: 1
direction: direction/forward
top level: BBa_I739104
start: 64
end: 101
orientation: inline

Scar
type: SequenceAnnotation
persistent identity: annotation1947892
display id: annotation1947892
version: 1
top level: BBa_I739104
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I739104/annotation1947892/range1947892/1

https://synbiohub.org/public/igem/BBa_I739104/annotation1947892/range1947892/1
type: Range
persistent identity: range1947892
display id: range1947892
version: 1
direction: direction/forward
top level: BBa_I739104
start: 56
end: 63
orientation: inline

OR1
type: SequenceAnnotation
persistent identity: annotation1947894
display id: annotation1947894
version: 1
top level: BBa_I739104
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I739104/annotation1947894/range1947894/1

https://synbiohub.org/public/igem/BBa_I739104/annotation1947894/range1947894/1
type: Range
persistent identity: range1947894
display id: range1947894
version: 1
direction: direction/forward
top level: BBa_I739104
start: 83
end: 101
orientation: inline

OR1
type: SequenceAnnotation
persistent identity: annotation1947893
display id: annotation1947893
version: 1
top level: BBa_I739104
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I739104/annotation1947893/range1947893/1

https://synbiohub.org/public/igem/BBa_I739104/annotation1947893/range1947893/1
type: Range
persistent identity: range1947893
display id: range1947893
version: 1
direction: direction/forward
top level: BBa_I739104
start: 64
end: 82
orientation: inline

R0062
type: SequenceAnnotation
persistent identity: annotation1947891
display id: annotation1947891
version: 1
top level: BBa_I739104
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I739104/annotation1947891/range1947891/1

https://synbiohub.org/public/igem/BBa_I739104/annotation1947891/range1947891/1
type: Range
persistent identity: range1947891
display id: range1947891
version: 1
direction: direction/forward
top level: BBa_I739104
start: 1
end: 55
orientation: inline

BBa_J34800
type: ComponentDefinition
persistent identity: BBa J34800
display id: BBa_J34800
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter tetracyclin inducible
creator: Alexandra Choutko
created: 2006-10-25T11:00:00Z
modified: 2015-08-31T04:08:46Z
discontinued: false
dominant: false
group u list: _62_
m user id: 0
owner id: 1085
owning group id: 62
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: promoter
mutable notes: no
mutable provenance: no
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J34800_sequence/1

Pmra
type: ComponentDefinition
persistent identity: BBa S03331
display id: BBa_S03331
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: --Specify Parts List--
creator: Simone Macmil
created: 2005-09-01T11:00:00Z
modified: 2015-05-08T01:14:21Z
discontinued: false
dominant: false
group u list: _39_
m user id: 0
owner id: 318
owning group id: 39
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Promoter for cell division genes ftsI and ftsW
mutable notes: BBa_S03331is based on the reguatory region preceding cell division genes in the mra cluster and includes the biobrick head and tail restriction sites.
mutable provenance: A promoter for the first nine genes of the Escherichia coli mra cluster of cell division and cell envelope biosynthesis genes, including ftsI and ftsW. Hara H, Yasuda S, Horiuchi K and Park JT. Journal of Bacteriology (1997) 179: 5802-5811
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_S03331_sequence/1

BBa_J61146
type: ComponentDefinition
persistent identity: BBa J61146
display id: BBa_J61146
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: {rbs1} Library Member in BBb
creator: John Anderson
created: 2007-09-03T11:00:00Z
modified: 2015-08-31T01:56:25Z
discontinued: false
dominant: false
experience: experience/None
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: {{BBbRBS1Family}}
mutable notes: {{BerkiGEM2007-Biobrick2.0Intro}}
mutable provenance: Synthetic
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J61146_sequence/1

U037O11+D0
type: ComponentDefinition
persistent identity: BBa I732448
display id: BBa_I732448
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Family Member (U037O11+D062O22)
creator: Zhan Jian
created: 2007-10-13T11:00:00Z
modified: 2015-08-31T04:07:58Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732448_sequence/1

BBa_K094120
type: ComponentDefinition
persistent identity: BBa K094120
display id: BBa_K094120
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pLacI/ara-1
creator: Shi Lei
created: 2008-10-12T11:00:00Z
modified: 2015-05-08T01:08:40Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _255_
m user id: 0
owner id: 2589
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This promoter can be repressed by lacI protein and induced by either IPTG or arabinose.
mutable notes: IPTG has better effect on this promoter than arabinose
mutable provenance: Standard pLacI/ara-1 promoter
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: pLac/ara-1
sequence: https://synbiohub.org/public/igem/BBa_K094120_sequence/1

pLac/ara-1
type: SequenceAnnotation
persistent identity: annotation1980631
display id: annotation1980631
version: 1
top level: BBa_K094120
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K094120/annotation1980631/range1980631/1

https://synbiohub.org/public/igem/BBa_K094120/annotation1980631/range1980631/1
type: Range
persistent identity: range1980631
display id: range1980631
version: 1
direction: direction/forward
top level: BBa_K094120
start: 1
end: 103
orientation: inline

cI+luxR
type: ComponentDefinition
persistent identity: BBa R0065
display id: BBa_R0065
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter (lambda cI and luxR regulated -- hybrid)
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:15Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: cI repressor negatively regulates this promoter and LuxR activates its transcription.The effect of cI is dominant over LuxR. This part is based on the LuxR and cI repressor regulated hybrid promoter designed and tested by Ron Weiss. It requires the binding of two cI repressor dimers for maximal repression and contains two cI repressor binding sites namely, OR1 and OR2. This promoter is leaky in the sense that 'some' transcription is seen in the absence of both cI and LuxR. LuxI cI activity of promoter + + zero + - maximum - + zero - - leaky (no quantitative information)
mutable notes: This part was designed based on the LuxR and cI repressor regulated hybrid promoter tested by Ron Weiss and the LuxR-LuxICDABE sequence annotated by Tom Knight AF170104 .
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -35
sequence annotation: OR2 cI
sequence annotation: -10
sequence annotation: BBa_R0065
sequence annotation: OR1 cI
sequence annotation: lux p(R) start
sequence annotation: Lux Box
sequence: https://synbiohub.org/public/igem/BBa_R0065_sequence/1

-35
type: SequenceAnnotation
persistent identity: annotation1986779
display id: annotation1986779
version: 1
top level: cI+luxR
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0065/annotation1986779/range1986779/1

https://synbiohub.org/public/igem/BBa_R0065/annotation1986779/range1986779/1
type: Range
persistent identity: range1986779
display id: range1986779
version: 1
direction: direction/forward
top level: cI+luxR
start: 71
end: 76
orientation: inline

OR2 cI
type: SequenceAnnotation
persistent identity: annotation1986777
display id: annotation1986777
version: 1
top level: cI+luxR
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0065/annotation1986777/range1986777/1

https://synbiohub.org/public/igem/BBa_R0065/annotation1986777/range1986777/1
type: Range
persistent identity: range1986777
display id: range1986777
version: 1
direction: direction/forward
top level: cI+luxR
start: 57
end: 73
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1986776
display id: annotation1986776
version: 1
top level: cI+luxR
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0065/annotation1986776/range1986776/1

https://synbiohub.org/public/igem/BBa_R0065/annotation1986776/range1986776/1
type: Range
persistent identity: range1986776
display id: range1986776
version: 1
direction: direction/forward
top level: cI+luxR
start: 47
end: 52
orientation: inline

BBa_R0065
type: SequenceAnnotation
persistent identity: annotation1986774
display id: annotation1986774
version: 1
top level: cI+luxR
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_R0065/annotation1986774/range1986774/1

https://synbiohub.org/public/igem/BBa_R0065/annotation1986774/range1986774/1
type: Range
persistent identity: range1986774
display id: range1986774
version: 1
direction: direction/forward
top level: cI+luxR
start: 1
end: 97
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1986781
display id: annotation1986781
version: 1
top level: cI+luxR
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0065/annotation1986781/range1986781/1

https://synbiohub.org/public/igem/BBa_R0065/annotation1986781/range1986781/1
type: Range
persistent identity: range1986781
display id: range1986781
version: 1
direction: direction/forward
top level: cI+luxR
start: 94
end: 97
orientation: inline

OR1 cI
type: SequenceAnnotation
persistent identity: annotation1986780
display id: annotation1986780
version: 1
top level: cI+luxR
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0065/annotation1986780/range1986780/1

https://synbiohub.org/public/igem/BBa_R0065/annotation1986780/range1986780/1
type: Range
persistent identity: range1986780
display id: range1986780
version: 1
direction: direction/forward
top level: cI+luxR
start: 81
end: 97
orientation: inline

lux p(R) start
type: SequenceAnnotation
persistent identity: annotation1986778
display id: annotation1986778
version: 1
top level: cI+luxR
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0065/annotation1986778/range1986778/1

https://synbiohub.org/public/igem/BBa_R0065/annotation1986778/range1986778/1
type: Range
persistent identity: range1986778
display id: range1986778
version: 1
direction: direction/forward
top level: cI+luxR
start: 58
end: 58
orientation: inline

Lux Box
type: SequenceAnnotation
persistent identity: annotation1986775
display id: annotation1986775
version: 1
top level: cI+luxR
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0065/annotation1986775/range1986775/1

https://synbiohub.org/public/igem/BBa_R0065/annotation1986775/range1986775/1
type: Range
persistent identity: range1986775
display id: range1986775
version: 1
direction: direction/forward
top level: cI+luxR
start: 6
end: 25
orientation: inline

BBa_K091100
type: ComponentDefinition
persistent identity: BBa K091100
display id: BBa_K091100
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pLac_lux hybrid promoter
creator: Pallavi Penumetcha
created: 2008-05-28T11:00:00Z
modified: 2015-05-08T01:08:37Z
discontinued: false
dominant: false
experience: experience/None
group u list: _191_
m user id: 0
owner id: 2993
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This promoter is repressed by lacI and luxR with HSL. It contains a lux box and a lacI binding site.
mutable notes: Deleted the CAP binding site in R0010.
mutable provenance: R0010 modified with the insertion of a lux box in between -35 and -10.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: lux box
sequence annotation: -10
sequence annotation: lacI binding site
sequence annotation: -35
sequence: https://synbiohub.org/public/igem/BBa_K091100_sequence/1

lux box
type: SequenceAnnotation
persistent identity: annotation1963335
display id: annotation1963335
version: 1
top level: BBa_K091100
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K091100/annotation1963335/range1963335/1

https://synbiohub.org/public/igem/BBa_K091100/annotation1963335/range1963335/1
type: Range
persistent identity: range1963335
display id: range1963335
version: 1
direction: direction/forward
top level: BBa_K091100
start: 16
end: 33
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1963334
display id: annotation1963334
version: 1
top level: BBa_K091100
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K091100/annotation1963334/range1963334/1

https://synbiohub.org/public/igem/BBa_K091100/annotation1963334/range1963334/1
type: Range
persistent identity: range1963334
display id: range1963334
version: 1
direction: direction/forward
top level: BBa_K091100
start: 34
end: 39
orientation: inline

lacI binding site
type: SequenceAnnotation
persistent identity: annotation1963336
display id: annotation1963336
version: 1
top level: BBa_K091100
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K091100/annotation1963336/range1963336/1

https://synbiohub.org/public/igem/BBa_K091100/annotation1963336/range1963336/1
type: Range
persistent identity: range1963336
display id: range1963336
version: 1
direction: direction/forward
top level: BBa_K091100
start: 40
end: 74
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1963333
display id: annotation1963333
version: 1
top level: BBa_K091100
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K091100/annotation1963333/range1963333/1

https://synbiohub.org/public/igem/BBa_K091100/annotation1963333/range1963333/1
type: Range
persistent identity: range1963333
display id: range1963333
version: 1
direction: direction/forward
top level: BBa_K091100
start: 10
end: 15
orientation: inline

BBa_J61121
type: ComponentDefinition
persistent identity: BBa J61121
display id: BBa_J61121
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Ribosome Binding Site Family Member
creator: John Anderson
created: 2007-04-24T11:00:00Z
modified: 2015-08-31T01:59:49Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: false
mutable description: {{JCA_Arkin_RBSFamily}}
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J61121_sequence/1

BBa_J64067
type: ComponentDefinition
persistent identity: BBa J64067
display id: BBa_J64067
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LuxR+3OC6HSL independent R0065
creator: Jeffrey J Tabor
created: 2007-12-12T12:00:00Z
modified: 2015-05-08T01:08:17Z
discontinued: false
dominant: false
experience: experience/None
group u list: _98_
m user id: 0
owner id: 88
owning group id: 98
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: The -35 box of R0065 has been modified to promote constitutive (LuxR+3OC6HSL-independent) gene expression. An extended -10 sequence has also been added to increase absolute transcription. The promoter still contains the operators OR1 and OR2 such that it retains its sensitivity to CI.
mutable notes: see above
mutable provenance: modified from R0065
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: extended -10
sequence annotation: OR2
sequence annotation: +1
sequence annotation: OR1
sequence annotation: -10
sequence annotation: -35
sequence annotation: lux box
sequence: https://synbiohub.org/public/igem/BBa_J64067_sequence/1

extended -10
type: SequenceAnnotation
persistent identity: annotation1959019
display id: annotation1959019
version: 1
top level: BBa_J64067
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_J64067/annotation1959019/range1959019/1

https://synbiohub.org/public/igem/BBa_J64067/annotation1959019/range1959019/1
type: Range
persistent identity: range1959019
display id: range1959019
version: 1
direction: direction/forward
top level: BBa_J64067
start: 43
end: 46
orientation: inline

OR2
type: SequenceAnnotation
persistent identity: annotation1959023
display id: annotation1959023
version: 1
top level: BBa_J64067
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_J64067/annotation1959023/range1959023/1

https://synbiohub.org/public/igem/BBa_J64067/annotation1959023/range1959023/1
type: Range
persistent identity: range1959023
display id: range1959023
version: 1
direction: direction/forward
top level: BBa_J64067
start: 58
end: 75
orientation: inline

+1
type: SequenceAnnotation
persistent identity: annotation1959022
display id: annotation1959022
version: 1
top level: BBa_J64067
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J64067/annotation1959022/range1959022/1

https://synbiohub.org/public/igem/BBa_J64067/annotation1959022/range1959022/1
type: Range
persistent identity: range1959022
display id: range1959022
version: 1
direction: direction/forward
top level: BBa_J64067
start: 59
end: 59
orientation: inline

OR1
type: SequenceAnnotation
persistent identity: annotation1959024
display id: annotation1959024
version: 1
top level: BBa_J64067
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J64067/annotation1959024/range1959024/1

https://synbiohub.org/public/igem/BBa_J64067/annotation1959024/range1959024/1
type: Range
persistent identity: range1959024
display id: range1959024
version: 1
direction: direction/forward
top level: BBa_J64067
start: 82
end: 98
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1959020
display id: annotation1959020
version: 1
top level: BBa_J64067
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_J64067/annotation1959020/range1959020/1

https://synbiohub.org/public/igem/BBa_J64067/annotation1959020/range1959020/1
type: Range
persistent identity: range1959020
display id: range1959020
version: 1
direction: direction/forward
top level: BBa_J64067
start: 48
end: 53
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1959018
display id: annotation1959018
version: 1
top level: BBa_J64067
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_J64067/annotation1959018/range1959018/1

https://synbiohub.org/public/igem/BBa_J64067/annotation1959018/range1959018/1
type: Range
persistent identity: range1959018
display id: range1959018
version: 1
direction: direction/forward
top level: BBa_J64067
start: 25
end: 30
orientation: inline

lux box
type: SequenceAnnotation
persistent identity: annotation1959021
display id: annotation1959021
version: 1
top level: BBa_J64067
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_J64067/annotation1959021/range1959021/1

https://synbiohub.org/public/igem/BBa_J64067/annotation1959021/range1959021/1
type: Range
persistent identity: range1959021
display id: range1959021
version: 1
direction: direction/forward
top level: BBa_J64067
start: 6
end: 26
orientation: inline

eGFP-PTS
type: ComponentDefinition
persistent identity: BBa K678017
display id: BBa_K678017
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: eGFP-PTS-module
creator: DTU-Denmark-2
created: 2011-09-12T11:00:00Z
modified: 2015-05-08T01:13:04Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _882_
m user id: 0
owner id: 10556
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: to be announced
mutable notes: This part was designed for the Plug'n'Play with DNA assembly standard (RFC 80), therefore this part contains tails at the 5' and 3' ends as shown on the figure below.
mutable provenance: The Danish Cancer society
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: Kozak
sequence annotation: eGFP
sequence annotation: Linker 2
sequence annotation: PTS
sequence: https://synbiohub.org/public/igem/BBa_K678017_sequence/1

Kozak
type: SequenceAnnotation
persistent identity: annotation2127795
display id: annotation2127795
version: 1
top level: eGFP-PTS
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K678017/annotation2127795/range2127795/1

https://synbiohub.org/public/igem/BBa_K678017/annotation2127795/range2127795/1
type: Range
persistent identity: range2127795
display id: range2127795
version: 1
direction: direction/forward
top level: eGFP-PTS
start: 9
end: 19
orientation: inline

eGFP
type: SequenceAnnotation
persistent identity: annotation2127650
display id: annotation2127650
version: 1
top level: eGFP-PTS
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K678017/annotation2127650/range2127650/1

https://synbiohub.org/public/igem/BBa_K678017/annotation2127650/range2127650/1
type: Range
persistent identity: range2127650
display id: range2127650
version: 1
direction: direction/forward
top level: eGFP-PTS
start: 16
end: 717
orientation: inline

Linker 2
type: SequenceAnnotation
persistent identity: annotation2127652
display id: annotation2127652
version: 1
top level: eGFP-PTS
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K678017/annotation2127652/range2127652/1

https://synbiohub.org/public/igem/BBa_K678017/annotation2127652/range2127652/1
type: Range
persistent identity: range2127652
display id: range2127652
version: 1
direction: direction/forward
top level: eGFP-PTS
start: 1
end: 8
orientation: inline

PTS
type: SequenceAnnotation
persistent identity: annotation2127651
display id: annotation2127651
version: 1
top level: eGFP-PTS
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K678017/annotation2127651/range2127651/1

https://synbiohub.org/public/igem/BBa_K678017/annotation2127651/range2127651/1
type: Range
persistent identity: range2127651
display id: range2127651
version: 1
direction: direction/forward
top level: eGFP-PTS
start: 712
end: 744
orientation: inline

BBa_B0039
type: ComponentDefinition
persistent identity: BBa B0039
display id: BBa_B0039
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS 2
creator: Josh Michener
created: 2005-12-20T12:00:00Z
modified: 2015-08-31T04:07:20Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_
m user id: 0
owner id: 253
owning group id: 6
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Modified version of B0031 from the Voigt Lab. Not currently available as an independent part (only in a tet inverter).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_B0039_sequence/1

BBa_J61140
type: ComponentDefinition
persistent identity: BBa J61140
display id: BBa_J61140
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: {rbs1} Library Member in BBb
creator: John Anderson
created: 2007-09-03T11:00:00Z
modified: 2015-08-31T01:59:49Z
discontinued: false
dominant: false
experience: experience/None
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: {{BBbRBS1Family}}
mutable notes: {{BerkiGEM2007-Biobrick2.0Intro}}
mutable provenance: Synthetic
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J61140_sequence/1

Pnot(O1wt1
type: ComponentDefinition
persistent identity: BBa I732200
display id: BBa_I732200
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: NOT Gate Promoter Family Member (D001O1wt1)
creator: Zhan Jian
created: 2007-10-13T11:00:00Z
modified: 2015-08-31T04:07:57Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732200_sequence/1

Lock 1
type: ComponentDefinition
persistent identity: BBa J01010
display id: BBa_J01010
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Riboregulator Lock 1
creator: Golden Bear
created: 2005-11-05T12:00:00Z
modified: 2015-08-31T04:08:12Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _13_
m user id: 0
owner id: 395
owning group id: 13
sample status: In stock
status: status/Available
bookmark: false
mutable description: Biobricked version of Isaacs' riboregulator cis repressed lock, crR12.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000834
role: partType/RNA
sequence: https://synbiohub.org/public/igem/BBa_J01010_sequence/1

BBa_I739101
type: ComponentDefinition
persistent identity: BBa I739101
display id: BBa_I739101
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Double Promoter (constitutive / TetR, negative)
creator: Stefan Luzi
created: 2007-10-11T11:00:00Z
modified: 2015-08-31T04:08:01Z
discontinued: false
dominant: false
experience: experience/None
group u list: _124_
m user id: 0
owner id: 2183
owning group id: 62
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: sdfasdf
mutable notes: asdf
mutable provenance: Synthetic DNA
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: R0040 (2x TetR 1)
sequence annotation: TetR 1
sequence annotation: J23100
sequence annotation: Scar
sequence: https://synbiohub.org/public/igem/BBa_I739101_sequence/1

R0040 (2x TetR 1)
type: SequenceAnnotation
persistent identity: annotation1946257
display id: annotation1946257
version: 1
top level: BBa_I739101
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_I739101/annotation1946257/range1946257/1

https://synbiohub.org/public/igem/BBa_I739101/annotation1946257/range1946257/1
type: Range
persistent identity: range1946257
display id: range1946257
version: 1
direction: direction/forward
top level: BBa_I739101
start: 44
end: 83
orientation: inline

TetR 1
type: SequenceAnnotation
persistent identity: annotation1946101
display id: annotation1946101
version: 1
top level: BBa_I739101
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I739101/annotation1946101/range1946101/1

https://synbiohub.org/public/igem/BBa_I739101/annotation1946101/range1946101/1
type: Range
persistent identity: range1946101
display id: range1946101
version: 1
direction: direction/forward
top level: BBa_I739101
start: 64
end: 83
orientation: inline

J23100
type: SequenceAnnotation
persistent identity: annotation1946100
display id: annotation1946100
version: 1
top level: BBa_I739101
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I739101/annotation1946100/range1946100/1

https://synbiohub.org/public/igem/BBa_I739101/annotation1946100/range1946100/1
type: Range
persistent identity: range1946100
display id: range1946100
version: 1
direction: direction/forward
top level: BBa_I739101
start: 1
end: 35
orientation: inline

Scar
type: SequenceAnnotation
persistent identity: annotation1946099
display id: annotation1946099
version: 1
top level: BBa_I739101
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I739101/annotation1946099/range1946099/1

https://synbiohub.org/public/igem/BBa_I739101/annotation1946099/range1946099/1
type: Range
persistent identity: range1946099
display id: range1946099
version: 1
direction: direction/forward
top level: BBa_I739101
start: 36
end: 43
orientation: inline

TetR 1
type: SequenceAnnotation
persistent identity: annotation1946102
display id: annotation1946102
version: 1
top level: BBa_I739101
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I739101/annotation1946102/range1946102/1

https://synbiohub.org/public/igem/BBa_I739101/annotation1946102/range1946102/1
type: Range
persistent identity: range1946102
display id: range1946102
version: 1
direction: direction/forward
top level: BBa_I739101
start: 44
end: 63
orientation: inline

BBa_B0078
type: ComponentDefinition
persistent identity: BBa B0078
display id: BBa_B0078
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Specialized RBS
creator: Bartholomew Canton
created: 2006-05-01T11:00:00Z
modified: 2015-08-31T04:07:21Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_6_
m user id: 0
owner id: 135
owning group id: 6
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_B0078_sequence/1

BBa_J23114
type: ComponentDefinition
persistent identity: BBa J23114
display id: BBa_J23114
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: constitutive promoter family member
creator: John Anderson
created: 2006-08-16T11:00:00Z
modified: 2015-08-31T04:08:40Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: Later
mutable notes: N/A
mutable provenance: Later
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J23114_sequence/1

BBa_M31519
type: ComponentDefinition
persistent identity: BBa M31519
display id: BBa_M31519
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Modified promoter sequence of g3.
creator: Han Zhu
created: 2007-02-27T12:00:00Z
modified: 2015-05-08T01:14:00Z
discontinued: false
dominant: false
group u list: _102_
m user id: 0
owner id: 1377
owning group id: 102
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is the promoter sequence of g3 which has been separated from g8. It also includes the restriction enzyme EcoRV in front.
mutable notes: This part used in conjunction with BBa_M131520 allows g8 and promoter of g3 to be separate from each other, therefore allowing free regulation of g3 with no consequences on g8.
mutable provenance: GATATCaattcacctcgaaagcaagctgataaaccgatacaattaaaggctcct
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: BmtI
sequence annotation: promoter-g3
sequence annotation: EcoRV
sequence: https://synbiohub.org/public/igem/BBa_M31519_sequence/1

BmtI
type: SequenceAnnotation
persistent identity: annotation1919691
display id: annotation1919691
version: 1
top level: BBa_M31519
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_M31519/annotation1919691/range1919691/1

https://synbiohub.org/public/igem/BBa_M31519/annotation1919691/range1919691/1
type: Range
persistent identity: range1919691
display id: range1919691
version: 1
direction: direction/forward
top level: BBa_M31519
start: 54
end: 60
orientation: inline

promoter-g3
type: SequenceAnnotation
persistent identity: annotation1915216
display id: annotation1915216
version: 1
top level: BBa_M31519
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_M31519/annotation1915216/range1915216/1

https://synbiohub.org/public/igem/BBa_M31519/annotation1915216/range1915216/1
type: Range
persistent identity: range1915216
display id: range1915216
version: 1
direction: direction/forward
top level: BBa_M31519
start: 7
end: 54
orientation: inline

EcoRV
type: SequenceAnnotation
persistent identity: annotation1915217
display id: annotation1915217
version: 1
top level: BBa_M31519
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_M31519/annotation1915217/range1915217/1

https://synbiohub.org/public/igem/BBa_M31519/annotation1915217/range1915217/1
type: Range
persistent identity: range1915217
display id: range1915217
version: 1
direction: direction/forward
top level: BBa_M31519
start: 1
end: 6
orientation: inline

BBa_K1585113
type: ComponentDefinition
persistent identity: BBa K1585113
display id: BBa_K1585113
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Anderson Promoter with lacI binding site
creator: Tobias Schwanemann
created: 2015-08-31T11:00:00Z
modified: 2015-09-01T02:09:36Z
discontinued: false
dominant: false
experience: experience/Fails
group u list: _2002_
m user id: 24474
owner id: 24474
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Promoters of the Anderson Promoter Collection with lacI binding site derived from [http://parts.igem.org/Part:BBa_R0010 R_0010] to make them [http://openwetware.org/wiki/IPTG IPTG] iducible.
mutable notes: We choosed lacI-binding-site of part BBa_R0010 because we assumed older parts would be more reliable. The part is shipped in backbone [http://parts.igem.org/Part:BBa_J61002 J61002] because the functionality is directly visible by adding IPTG. RFP ([http://parts.igem.org/Part:BBa_E1010 E1010]) should get expressed then.
mutable provenance: Assambly of [http://parts.igem.org/Part:BBa_J23110 J23110] and lacI-binding-site of [http://parts.igem.org/Part:BBa_R0010 R_0010] The "lacI binding site" of Part [http://parts.igem.org/Part:BBa_R0010 R_0010] differs from lacO from Part [http://parts.igem.org/Part:BBa_J33207 BBa_J33207].
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
component: BBa_J23113
component: BBa_K1585999
sequence annotation: BBa_J23113
sequence annotation: BBa_K1585999
sequence: https://synbiohub.org/public/igem/BBa_K1585113_sequence/1

BBa_J23113
type: Component
persistent identity: component2441702
display id: component2441702
version: 1
top level: BBa_K1585113
access: public
definition: BBa_J23113

BBa_K1585999
type: Component
persistent identity: component2441704
display id: component2441704
version: 1
top level: BBa_K1585113
access: public
definition: https://synbiohub.org/public/igem/BBa_K1585999/1

BBa_J23113
type: SequenceAnnotation
persistent identity: annotation2441702
display id: annotation2441702
version: 1
top level: BBa_K1585113
location: https://synbiohub.org/public/igem/BBa_K1585113/annotation2441702/range2441702/1
component: BBa_J23113

https://synbiohub.org/public/igem/BBa_K1585113/annotation2441702/range2441702/1
type: Range
persistent identity: range2441702
display id: range2441702
version: 1
direction: direction/reverse
top level: BBa_K1585113
start: 1
end: 35
orientation: inline

BBa_K1585999
type: SequenceAnnotation
persistent identity: annotation2441704
display id: annotation2441704
version: 1
top level: BBa_K1585113
location: https://synbiohub.org/public/igem/BBa_K1585113/annotation2441704/range2441704/1
component: BBa_K1585999

https://synbiohub.org/public/igem/BBa_K1585113/annotation2441704/range2441704/1
type: Range
persistent identity: range2441704
display id: range2441704
version: 1
direction: direction/reverse
top level: BBa_K1585113
start: 44
end: 78
orientation: inline

BBa_K116500
type: ComponentDefinition
persistent identity: BBa K116500
display id: BBa_K116500
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: OmpF promoter that is activated or repressesed by OmpR according to osmolarity.
creator: Chun-Ju Yang
created: 2008-10-28T12:00:00Z
modified: 2015-05-08T01:09:33Z
discontinued: false
dominant: false
group u list: _210_
m user id: 0
owner id: 2083
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Promoter OmpF is activated by phosphorylated OmpR at low osmolarity, and is repressesed by phosphorylated OmpR at high osmolarity.In Escherichia coli, osmoregulation is mediated in part by the actions of such a two-component system consisting of EnvZ and OmpR. These proteins act to control the relative levels of the outer membrane porin genes, ompF and ompC. At low osmo larity, OmpF predominates in the outer membrane, while at high osmolarity the OmpF porin is replaced by OmpC.OmpF has a larger pore and a faster ??ow rate than OmpC. Reference: K. Mattison, R. Oropeza, N. Byers, L. J. Kenney, J Mol Biol 315, 497 (Jan 25, 2002).
mutable notes: The osmolarity range of OmpF sensing still have to be confirmed
mutable provenance: Promoter OmpF is activated by phosphorylated OmpR at low osmolarity, and is repressesed by phosphorylated OmpR at high osmolarity.In Escherichia coli, osmoregulation is mediated in part by the actions of such a two-component system consisting of EnvZ and OmpR. These proteins act to control the relative levels of the outer membrane porin genes, ompF and ompC. At low osmo larity, OmpF predominates in the outer membrane, while at high osmolarity the OmpF porin is replaced by OmpC.OmpF has a larger pore and a faster ??ow rate than OmpC.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: OmpR binding
sequence: https://synbiohub.org/public/igem/BBa_K116500_sequence/1

OmpR binding
type: SequenceAnnotation
persistent identity: annotation1994040
display id: annotation1994040
version: 1
top level: BBa_K116500
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K116500/annotation1994040/range1994040/1

https://synbiohub.org/public/igem/BBa_K116500/annotation1994040/range1994040/1
type: Range
persistent identity: range1994040
display id: range1994040
version: 1
direction: direction/forward
top level: BBa_K116500
start: 27
end: 88
orientation: inline

BBa_K086024
type: ComponentDefinition
persistent identity: BBa K086024
display id: BBa_K086024
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: modified Lutz-Bujard LacO promoter,with alternative sigma factor σ28
creator: Gairik Sachdeva
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:08:36Z
discontinued: false
dominant: false
group u list: _197_
m user id: 0
owner id: 2578
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: modified Lutz-Bujard LacO promoter,with alternative sigma factor σ28
mutable notes: cleaned
mutable provenance: Lac O and sigma 28 binding sites' conserved sequences
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: P24_3
sequence: https://synbiohub.org/public/igem/BBa_K086024_sequence/1

P24_3
type: SequenceAnnotation
persistent identity: annotation1995758
display id: annotation1995758
version: 1
top level: BBa_K086024
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K086024/annotation1995758/range1995758/1

https://synbiohub.org/public/igem/BBa_K086024/annotation1995758/range1995758/1
type: Range
persistent identity: range1995758
display id: range1995758
version: 1
direction: direction/forward
top level: BBa_K086024
start: 1
end: 55
orientation: inline

BBa_K1486021
type: ComponentDefinition
persistent identity: BBa K1486021
display id: BBa_K1486021
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Arabinose promoter + rLuc[1] + rLuc[2]
creator: EPFL iGem team 2014
created: 2014-09-14T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This part contains both fragments of the split renilla luciferase, each of them preceded by an Elowitz RBS.
mutable notes: to edit
mutable provenance: to edit
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0034
component: BBa_K1486020
component: BBa_K1486019
component: BBa_I0500
sequence annotation: BBa_I0500
sequence annotation: BBa_K1486020
sequence annotation: BBa_B0034
sequence annotation: BBa_K1486019
sequence: https://synbiohub.org/public/igem/BBa_K1486021_sequence/1

BBa_B0034
type: Component
persistent identity: component2383466
display id: component2383466
version: 1
top level: BBa_K1486021
access: public
definition: BBa_B0034

BBa_K1486020
type: Component
persistent identity: component2383467
display id: component2383467
version: 1
top level: BBa_K1486021
access: public
definition: BBa_K1486020

BBa_B0034
type: Component
persistent identity: component2383463
display id: component2383463
version: 1
top level: BBa_K1486021
access: public
definition: BBa_B0034

BBa_K1486019
type: Component
persistent identity: component2383464
display id: component2383464
version: 1
top level: BBa_K1486021
access: public
definition: BBa_K1486019

BBa_I0500
type: Component
persistent identity: component2383461
display id: component2383461
version: 1
top level: BBa_K1486021
access: public
definition: pBad/araC

BBa_I0500
type: SequenceAnnotation
persistent identity: annotation2383461
display id: annotation2383461
version: 1
top level: BBa_K1486021
location: https://synbiohub.org/public/igem/BBa_K1486021/annotation2383461/range2383461/1
component: BBa_I0500

https://synbiohub.org/public/igem/BBa_K1486021/annotation2383461/range2383461/1
type: Range
persistent identity: range2383461
display id: range2383461
version: 1
direction: direction/reverse
top level: BBa_K1486021
start: 1
end: 1210
orientation: inline

BBa_K1486020
type: SequenceAnnotation
persistent identity: annotation2383467
display id: annotation2383467
version: 1
top level: BBa_K1486021
location: https://synbiohub.org/public/igem/BBa_K1486021/annotation2383467/range2383467/1
component: BBa_K1486020

https://synbiohub.org/public/igem/BBa_K1486021/annotation2383467/range2383467/1
type: Range
persistent identity: range2383467
display id: range2383467
version: 1
direction: direction/reverse
top level: BBa_K1486021
start: 1565
end: 2170
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2383466
display id: annotation2383466
version: 1
top level: BBa_K1486021
location: https://synbiohub.org/public/igem/BBa_K1486021/annotation2383466/range2383466/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1486021/annotation2383466/range2383466/1
type: Range
persistent identity: range2383466
display id: range2383466
version: 1
direction: direction/reverse
top level: BBa_K1486021
start: 1553
end: 1564
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2383463
display id: annotation2383463
version: 1
top level: BBa_K1486021
location: https://synbiohub.org/public/igem/BBa_K1486021/annotation2383463/range2383463/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1486021/annotation2383463/range2383463/1
type: Range
persistent identity: range2383463
display id: range2383463
version: 1
direction: direction/reverse
top level: BBa_K1486021
start: 1211
end: 1222
orientation: inline

BBa_K1486019
type: SequenceAnnotation
persistent identity: annotation2383464
display id: annotation2383464
version: 1
top level: BBa_K1486021
location: https://synbiohub.org/public/igem/BBa_K1486021/annotation2383464/range2383464/1
component: BBa_K1486019

https://synbiohub.org/public/igem/BBa_K1486021/annotation2383464/range2383464/1
type: Range
persistent identity: range2383464
display id: range2383464
version: 1
direction: direction/reverse
top level: BBa_K1486021
start: 1223
end: 1552
orientation: inline

BBa_B0064
type: ComponentDefinition
persistent identity: BBa B0064
display id: BBa_B0064
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Tuned RBS for Q04401
creator: Jason Kelly
created: 2008-02-23T12:00:00Z
modified: 2015-08-31T04:07:21Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_
m user id: 0
owner id: 2
owning group id: 84
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is a single bp change from B0034. It's strength is approximately 35% that of B0034.
mutable notes: .
mutable provenance: .
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_B0064_sequence/1

BBa_B0037
type: ComponentDefinition
persistent identity: BBa B0037
display id: BBa_B0037
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Specialized RBS
creator: Barry Canton
created: 2005-12-06T12:00:00Z
modified: 2015-08-31T04:07:20Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_6_
m user id: 0
owner id: 135
owning group id: 6
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: RBS specific to dedicated ribosomes. This RBS is a modified version of B0036. The spacing between the SD sequence and the start codon has been increased by 5 bases to give the same spacing as used by Brink et al. The 5 bases are the same as those used by Brink. That means they form the first five bases of an XbaI site and no negative affects of using these bases are known at present.
mutable provenance: Brink MF, Verbeet MP, de Boer HA (1995). Specialized ribosomes: highly specific translation in vivo of a single targetted mRNA species. Gene, 156, pp. 215-222
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_B0037_sequence/1

BBa_I741020
type: ComponentDefinition
persistent identity: BBa I741020
display id: BBa_I741020
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: promoter to xylF without CRP and several binding sites for xylR
creator: Lucien Weiss
created: 2007-06-20T11:00:00Z
modified: 2015-08-31T04:08:02Z
discontinued: false
dominant: false
experience: experience/Issues
group u list: _143_
m user id: 0
owner id: 1763
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: removed RBS crp binding and xylR binding sites
mutable provenance: ecoli k12 chromosome
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: XylR-Xylose transcriptional activator activates transcription
sequence annotation: Transcription start base
sequence annotation: xylFp
sequence: https://synbiohub.org/public/igem/BBa_I741020_sequence/1

XylR-Xylose transcriptional activator activates transcription
type: SequenceAnnotation
persistent identity: annotation1935180
display id: annotation1935180
version: 1
top level: BBa_I741020
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I741020/annotation1935180/range1935180/1

https://synbiohub.org/public/igem/BBa_I741020/annotation1935180/range1935180/1
type: Range
persistent identity: range1935180
display id: range1935180
version: 1
direction: direction/forward
top level: BBa_I741020
start: 95
end: 112
orientation: inline

XylR-Xylose transcriptional activator activates transcription
type: SequenceAnnotation
persistent identity: annotation1935182
display id: annotation1935182
version: 1
top level: BBa_I741020
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I741020/annotation1935182/range1935182/1

https://synbiohub.org/public/igem/BBa_I741020/annotation1935182/range1935182/1
type: Range
persistent identity: range1935182
display id: range1935182
version: 1
direction: direction/forward
top level: BBa_I741020
start: 116
end: 132
orientation: inline

Transcription start base
type: SequenceAnnotation
persistent identity: annotation1935183
display id: annotation1935183
version: 1
top level: BBa_I741020
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I741020/annotation1935183/range1935183/1

https://synbiohub.org/public/igem/BBa_I741020/annotation1935183/range1935183/1
type: Range
persistent identity: range1935183
display id: range1935183
version: 1
direction: direction/forward
top level: BBa_I741020
start: 165
end: 165
orientation: inline

xylFp
type: SequenceAnnotation
persistent identity: annotation1935181
display id: annotation1935181
version: 1
top level: BBa_I741020
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I741020/annotation1935181/range1935181/1

https://synbiohub.org/public/igem/BBa_I741020/annotation1935181/range1935181/1
type: Range
persistent identity: range1935181
display id: range1935181
version: 1
direction: direction/forward
top level: BBa_I741020
start: 105
end: 185
orientation: inline

BBa_K2042004
type: ComponentDefinition
persistent identity: BBa K2042004
display id: BBa_K2042004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: IPTG inducible promoter
creator: Robert Ram??rez Garc??a
created: 2016-09-01T11:00:00Z
modified: 2016-10-21T04:59:40Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2509_
m user id: 31392
owner id: 32514
owning group id: 9
status: status/Planning
bookmark: false
mutable description: A
mutable notes: A
mutable provenance: A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: LacO
sequence annotation: Ptrc regulatory
sequence annotation: -35
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_K2042004_sequence/1

LacO
type: SequenceAnnotation
persistent identity: annotation2489029
display id: annotation2489029
version: 1
top level: BBa_K2042004
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K2042004/annotation2489029/range2489029/1

https://synbiohub.org/public/igem/BBa_K2042004/annotation2489029/range2489029/1
type: Range
persistent identity: range2489029
display id: range2489029
version: 1
direction: direction/forward
top level: BBa_K2042004
start: 40
end: 60
orientation: inline

Ptrc regulatory
type: SequenceAnnotation
persistent identity: annotation2489024
display id: annotation2489024
version: 1
top level: BBa_K2042004
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K2042004/annotation2489024/range2489024/1

https://synbiohub.org/public/igem/BBa_K2042004/annotation2489024/range2489024/1
type: Range
persistent identity: range2489024
display id: range2489024
version: 1
direction: direction/forward
top level: BBa_K2042004
start: 4
end: 34
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2494919
display id: annotation2494919
version: 1
top level: BBa_K2042004
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K2042004/annotation2494919/range2494919/1

https://synbiohub.org/public/igem/BBa_K2042004/annotation2494919/range2494919/1
type: Range
persistent identity: range2494919
display id: range2494919
version: 1
direction: direction/forward
top level: BBa_K2042004
start: 4
end: 9
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2494920
display id: annotation2494920
version: 1
top level: BBa_K2042004
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K2042004/annotation2494920/range2494920/1

https://synbiohub.org/public/igem/BBa_K2042004/annotation2494920/range2494920/1
type: Range
persistent identity: range2494920
display id: range2494920
version: 1
direction: direction/forward
top level: BBa_K2042004
start: 27
end: 33
orientation: inline

BBa_I739106
type: ComponentDefinition
persistent identity: BBa I739106
display id: BBa_I739106
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Double Promoter (TetR, negative / P22 cII, negative)
creator: Stefan Luzi
created: 2007-10-15T11:00:00Z
modified: 2015-08-31T04:08:01Z
discontinued: false
dominant: false
group u list: _124_
m user id: 0
owner id: 2183
owning group id: 62
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: asdf
mutable notes: asdf
mutable provenance: Synthetic DNA
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: OR1
sequence annotation: R0040
sequence annotation: R0053 (2x OR1)
sequence: https://synbiohub.org/public/igem/BBa_I739106_sequence/1

OR1
type: SequenceAnnotation
persistent identity: annotation1947940
display id: annotation1947940
version: 1
top level: BBa_I739106
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I739106/annotation1947940/range1947940/1

https://synbiohub.org/public/igem/BBa_I739106/annotation1947940/range1947940/1
type: Range
persistent identity: range1947940
display id: range1947940
version: 1
direction: direction/forward
top level: BBa_I739106
start: 67
end: 84
orientation: inline

R0040
type: SequenceAnnotation
persistent identity: annotation1947938
display id: annotation1947938
version: 1
top level: BBa_I739106
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I739106/annotation1947938/range1947938/1

https://synbiohub.org/public/igem/BBa_I739106/annotation1947938/range1947938/1
type: Range
persistent identity: range1947938
display id: range1947938
version: 1
direction: direction/forward
top level: BBa_I739106
start: 1
end: 48
orientation: inline

OR1
type: SequenceAnnotation
persistent identity: annotation1947939
display id: annotation1947939
version: 1
top level: BBa_I739106
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I739106/annotation1947939/range1947939/1

https://synbiohub.org/public/igem/BBa_I739106/annotation1947939/range1947939/1
type: Range
persistent identity: range1947939
display id: range1947939
version: 1
direction: direction/forward
top level: BBa_I739106
start: 49
end: 66
orientation: inline

R0053 (2x OR1)
type: SequenceAnnotation
persistent identity: annotation1947941
display id: annotation1947941
version: 1
top level: BBa_I739106
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_I739106/annotation1947941/range1947941/1

https://synbiohub.org/public/igem/BBa_I739106/annotation1947941/range1947941/1
type: Range
persistent identity: range1947941
display id: range1947941
version: 1
direction: direction/forward
top level: BBa_I739106
start: 49
end: 84
orientation: inline

BBa_I751502
type: ComponentDefinition
persistent identity: BBa I751502
display id: BBa_I751502
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: plux-lac hybrid promoter
creator: Kenichiro Iwasaki
created: 2007-10-24T11:00:00Z
modified: 2015-08-31T04:08:06Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _151_
m user id: 0
owner id: 2188
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: sense TWO INPUTS, activation by AHL and repression by LacI.
mutable notes: Compared with existing BioBrick hybrid promoter R0065, this promoter is different in that its sequence is much shorter with lux box and -35 region overlapping, and that it is repressed by LacI, not cI. Also, the output is clearly detected as shown in Fig. 3.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: luxR binding site
sequence annotation: -35
sequence annotation: O1
sequence annotation: plac
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_I751502_sequence/1

luxR binding site
type: SequenceAnnotation
persistent identity: annotation1955564
display id: annotation1955564
version: 1
top level: BBa_I751502
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I751502/annotation1955564/range1955564/1

https://synbiohub.org/public/igem/BBa_I751502/annotation1955564/range1955564/1
type: Range
persistent identity: range1955564
display id: range1955564
version: 1
direction: direction/forward
top level: BBa_I751502
start: 1
end: 19
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1955560
display id: annotation1955560
version: 1
top level: BBa_I751502
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I751502/annotation1955560/range1955560/1

https://synbiohub.org/public/igem/BBa_I751502/annotation1955560/range1955560/1
type: Range
persistent identity: range1955560
display id: range1955560
version: 1
direction: direction/forward
top level: BBa_I751502
start: 20
end: 26
orientation: inline

O1
type: SequenceAnnotation
persistent identity: annotation1955562
display id: annotation1955562
version: 1
top level: BBa_I751502
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I751502/annotation1955562/range1955562/1

https://synbiohub.org/public/igem/BBa_I751502/annotation1955562/range1955562/1
type: Range
persistent identity: range1955562
display id: range1955562
version: 1
direction: direction/forward
top level: BBa_I751502
start: 27
end: 41
orientation: inline

O1
type: SequenceAnnotation
persistent identity: annotation1955563
display id: annotation1955563
version: 1
top level: BBa_I751502
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I751502/annotation1955563/range1955563/1

https://synbiohub.org/public/igem/BBa_I751502/annotation1955563/range1955563/1
type: Range
persistent identity: range1955563
display id: range1955563
version: 1
direction: direction/forward
top level: BBa_I751502
start: 49
end: 74
orientation: inline

plac
type: SequenceAnnotation
persistent identity: annotation1955559
display id: annotation1955559
version: 1
top level: BBa_I751502
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I751502/annotation1955559/range1955559/1

https://synbiohub.org/public/igem/BBa_I751502/annotation1955559/range1955559/1
type: Range
persistent identity: range1955559
display id: range1955559
version: 1
direction: direction/forward
top level: BBa_I751502
start: 20
end: 74
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1955561
display id: annotation1955561
version: 1
top level: BBa_I751502
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I751502/annotation1955561/range1955561/1

https://synbiohub.org/public/igem/BBa_I751502/annotation1955561/range1955561/1
type: Range
persistent identity: range1955561
display id: range1955561
version: 1
direction: direction/forward
top level: BBa_I751502
start: 42
end: 48
orientation: inline

BBa_K338029
type: ComponentDefinition
persistent identity: BBa K338029
display id: BBa_K338029
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: +OmpR, +(CinR-HSL) Double Promoter
creator: Lucas Hartsough
created: 2010-10-26T11:00:00Z
modified: 2015-05-08T01:12:07Z
discontinued: false
dominant: false
group u list: _454_
m user id: 0
owner id: 6013
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: An intermediate part designed to be used with the AND gate designed by Caltech iGEM '10: [http://2010.igem.org/Team:Caltech/Project Project Details].
mutable notes: Designed to be placed upstream of positive feedback mechanisms and desired output genes.
mutable provenance: The Registry of Standard Biological Parts
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_R0077
component: BBa_R0082
sequence annotation: BBa_R0077
sequence annotation: BBa_R0082
sequence: https://synbiohub.org/public/igem/BBa_K338029_sequence/1

BBa_R0077
type: Component
persistent identity: component2104982
display id: component2104982
version: 1
top level: BBa_K338029
access: public
definition: https://synbiohub.org/public/igem/BBa_R0077/1

BBa_R0082
type: Component
persistent identity: component2104977
display id: component2104977
version: 1
top level: BBa_K338029
access: public
definition: OmpR

BBa_R0077
type: SequenceAnnotation
persistent identity: annotation2104982
display id: annotation2104982
version: 1
top level: BBa_K338029
location: https://synbiohub.org/public/igem/BBa_K338029/annotation2104982/range2104982/1
component: BBa_R0077

https://synbiohub.org/public/igem/BBa_K338029/annotation2104982/range2104982/1
type: Range
persistent identity: range2104982
display id: range2104982
version: 1
direction: direction/reverse
top level: BBa_K338029
start: 117
end: 347
orientation: inline

BBa_R0082
type: SequenceAnnotation
persistent identity: annotation2104977
display id: annotation2104977
version: 1
top level: BBa_K338029
location: https://synbiohub.org/public/igem/BBa_K338029/annotation2104977/range2104977/1
component: BBa_R0082

https://synbiohub.org/public/igem/BBa_K338029/annotation2104977/range2104977/1
type: Range
persistent identity: range2104977
display id: range2104977
version: 1
direction: direction/reverse
top level: BBa_K338029
start: 1
end: 108
orientation: inline

BBa_I756015
type: ComponentDefinition
persistent identity: BBa I756015
display id: BBa_I756015
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CMV Promoter with lac operator sites
creator: Jack
created: 2007-08-15T11:00:00Z
modified: 2015-08-31T04:08:06Z
discontinued: false
dominant: false
experience: experience/None
group u list: _108_
m user id: 0
owner id: 2067
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: CMV Promoter with lac operator sites.
mutable notes: CMV promoter with inserted lac operator sites. Lac operator from POPI3Cat
mutable provenance: Promoter
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: Lac operator
sequence: https://synbiohub.org/public/igem/BBa_I756015_sequence/1

Lac operator
type: SequenceAnnotation
persistent identity: annotation1941580
display id: annotation1941580
version: 1
top level: BBa_I756015
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I756015/annotation1941580/range1941580/1

https://synbiohub.org/public/igem/BBa_I756015/annotation1941580/range1941580/1
type: Range
persistent identity: range1941580
display id: range1941580
version: 1
direction: direction/forward
top level: BBa_I756015
start: 513
end: 619
orientation: inline

BBa_J23150
type: ComponentDefinition
persistent identity: BBa J23150
display id: BBa_J23150
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: 1bp mutant from J23107
creator: Jason Kelly
created: 2008-02-11T12:00:00Z
modified: 2015-08-31T04:08:41Z
discontinued: false
dominant: true
group u list: _11_
m user id: 0
owner id: 2
owning group id: 84
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: .
mutable notes: .
mutable provenance: ..
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J23150_sequence/1

BBa_K076017
type: ComponentDefinition
persistent identity: BBa K076017
display id: BBa_K076017
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Ubc Promoter
creator: Available
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:08:32Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _215_
m user id: 0
owner id: 3466
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is a stable constitutive promoter.
mutable notes: Using this promoter allows for the constant expression of a reporter gene or a selectable antibiotic.
mutable provenance: This part was obtained from members of the Weiss Lab.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K076017_sequence/1

creC RBS
type: ComponentDefinition
persistent identity: BBa J64908
display id: BBa_J64908
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS for creC in e coli operon
creator: Ryan Ritterson
created: 2007-03-19T12:00:00Z
modified: 2015-05-08T01:08:17Z
discontinued: false
dominant: false
experience: experience/None
group u list: _98_
m user id: 0
owner id: 1422
owning group id: 98
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: The ribosomal binding site for gene creC in the E Coli cre operon.
mutable notes: natural sequence, no design
mutable provenance: E Coli K12 strain.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J64908_sequence/1

BBa_J72005
type: ComponentDefinition
persistent identity: BBa J72005
display id: BBa_J72005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: {Ptet} promoter in BBb
creator: John Anderson
created: 2008-07-15T11:00:00Z
modified: 2015-05-08T01:08:26Z
discontinued: false
dominant: false
group u list: _171_
m user id: 0
owner id: 483
owning group id: 171
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Equivalent part to r0040, but in BBb format. In house, part is referred to as pBca9145-Bca1091 {Ptet}. ---- This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at: [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
mutable notes: N/A
mutable provenance: PCR ca1091F/R on pSB1A2-r0040 (~100 bp, EcoRI/BamHI) Sub into pBca9145-Bca1089 (EcoRI/BamHI) Product is pBca9145-Bca1091 ---- ca1091F Forward EcoRI/BglII for Ptet part ttctggaattcatgAGATCTtccctatcagtgatagag ca1091R Reverse BamHI for Ptet part GtttGGATCCgtgctcagtatctctatc
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J72005_sequence/1

BBa_Z0251
type: ComponentDefinition
persistent identity: BBa Z0251
display id: BBa_Z0251
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: T7 strong promoter
creator: T7.2
created: 2005-04-04T11:00:00Z
modified: 2015-05-08T01:14:55Z
discontinued: false
dominant: false
experience: experience/None
group u list: _10_
m user id: 0
owner id: 250
owning group id: 10
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Promoter for T7 RNA polymerase
mutable provenance: bacteriophage T7
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: consensus sequence
sequence: https://synbiohub.org/public/igem/BBa_Z0251_sequence/1

consensus sequence
type: SequenceAnnotation
persistent identity: annotation1476333
display id: annotation1476333
version: 1
top level: BBa_Z0251
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_Z0251/annotation1476333/range1476333/1

https://synbiohub.org/public/igem/BBa_Z0251/annotation1476333/range1476333/1
type: Range
persistent identity: range1476333
display id: range1476333
version: 1
direction: direction/forward
top level: BBa_Z0251
start: 6
end: 28
orientation: inline

BBa_K1824891
type: ComponentDefinition
persistent identity: BBa K1824891
display id: BBa_K1824891
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: J23105+RBS
creator: Zixu Wang
created: 2015-09-09T11:00:00Z
modified: 2015-09-11T06:09:57Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2250_
m user id: 26000
owner id: 26000
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: J23105 plus B0034 is a regulatory part that can be used for both efficient transcription and translation. Just add any functional protein you want after it.
mutable notes: no special concern
mutable provenance: J23105 comes from constitutive promoter family from a small combinatorial library, B0034 is an RBS based on Elowitz repressilator.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1824890
component: BBa_B0034
component: BBa_J23105
component: BBa_K1824889
sequence annotation: BBa_J23105
sequence annotation: BBa_B0034
sequence annotation: BBa_K1824890
sequence annotation: BBa_K1824889
sequence: https://synbiohub.org/public/igem/BBa_K1824891_sequence/1

BBa_K1824890
type: Component
persistent identity: component2449106
display id: component2449106
version: 1
top level: BBa_K1824891
access: public
definition: https://synbiohub.org/public/igem/BBa_K1824890/1

BBa_B0034
type: Component
persistent identity: component2449105
display id: component2449105
version: 1
top level: BBa_K1824891
access: public
definition: BBa_B0034

BBa_J23105
type: Component
persistent identity: component2449102
display id: component2449102
version: 1
top level: BBa_K1824891
access: public
definition: BBa_J23105

BBa_K1824889
type: Component
persistent identity: component2449103
display id: component2449103
version: 1
top level: BBa_K1824891
access: public
definition: https://synbiohub.org/public/igem/BBa_K1824889/1

BBa_J23105
type: SequenceAnnotation
persistent identity: annotation2449102
display id: annotation2449102
version: 1
top level: BBa_K1824891
location: https://synbiohub.org/public/igem/BBa_K1824891/annotation2449102/range2449102/1
component: BBa_J23105

https://synbiohub.org/public/igem/BBa_K1824891/annotation2449102/range2449102/1
type: Range
persistent identity: range2449102
display id: range2449102
version: 1
direction: direction/reverse
top level: BBa_K1824891
start: 1
end: 35
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2449105
display id: annotation2449105
version: 1
top level: BBa_K1824891
location: https://synbiohub.org/public/igem/BBa_K1824891/annotation2449105/range2449105/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1824891/annotation2449105/range2449105/1
type: Range
persistent identity: range2449105
display id: range2449105
version: 1
direction: direction/reverse
top level: BBa_K1824891
start: 44
end: 55
orientation: inline

BBa_K1824890
type: SequenceAnnotation
persistent identity: annotation2449106
display id: annotation2449106
version: 1
top level: BBa_K1824891
location: https://synbiohub.org/public/igem/BBa_K1824891/annotation2449106/range2449106/1
component: BBa_K1824890

https://synbiohub.org/public/igem/BBa_K1824891/annotation2449106/range2449106/1
type: Range
persistent identity: range2449106
display id: range2449106
version: 1
direction: direction/reverse
top level: BBa_K1824891
start: 56
end: 61
orientation: inline

BBa_K1824889
type: SequenceAnnotation
persistent identity: annotation2449103
display id: annotation2449103
version: 1
top level: BBa_K1824891
location: https://synbiohub.org/public/igem/BBa_K1824891/annotation2449103/range2449103/1
component: BBa_K1824889

https://synbiohub.org/public/igem/BBa_K1824891/annotation2449103/range2449103/1
type: Range
persistent identity: range2449103
display id: range2449103
version: 1
direction: direction/reverse
top level: BBa_K1824891
start: 36
end: 43
orientation: inline

Zif23
type: ComponentDefinition
persistent identity: BBa R2001
display id: BBa_R2001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter, Zif23 regulated, test: after
creator: Ronny Krashinsky
created: 2004-01-29T12:00:00Z
modified: 2015-05-08T01:14:15Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Promoter with standard -10 and -35 sites. A symmetric Zif23 dimer target site is after the -10 site.
mutable notes: consensus sequences based on [Jensen98].
mutable provenance: synthetic
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: consensus
sequence annotation: -10
sequence annotation: Zif23
sequence annotation: (+1)
sequence annotation: -35
sequence: https://synbiohub.org/public/igem/BBa_R2001_sequence/1

consensus
type: SequenceAnnotation
persistent identity: annotation318135
display id: annotation318135
version: 1
top level: Zif23
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_R2001/annotation318135/range318135/1

https://synbiohub.org/public/igem/BBa_R2001/annotation318135/range318135/1
type: Range
persistent identity: range318135
display id: range318135
version: 1
direction: direction/forward
top level: Zif23
start: 1
end: 9
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation318133
display id: annotation318133
version: 1
top level: Zif23
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R2001/annotation318133/range318133/1

https://synbiohub.org/public/igem/BBa_R2001/annotation318133/range318133/1
type: Range
persistent identity: range318133
display id: range318133
version: 1
direction: direction/forward
top level: Zif23
start: 33
end: 38
orientation: inline

consensus
type: SequenceAnnotation
persistent identity: annotation318142
display id: annotation318142
version: 1
top level: Zif23
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_R2001/annotation318142/range318142/1

https://synbiohub.org/public/igem/BBa_R2001/annotation318142/range318142/1
type: Range
persistent identity: range318142
display id: range318142
version: 1
direction: direction/forward
top level: Zif23
start: 30
end: 32
orientation: inline

Zif23
type: SequenceAnnotation
persistent identity: annotation318136
display id: annotation318136
version: 1
top level: Zif23
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R2001/annotation318136/range318136/1

https://synbiohub.org/public/igem/BBa_R2001/annotation318136/range318136/1
type: Range
persistent identity: range318136
display id: range318136
version: 1
direction: direction/forward
top level: Zif23
start: 39
end: 52
orientation: inline

(+1)
type: SequenceAnnotation
persistent identity: annotation318134
display id: annotation318134
version: 1
top level: Zif23
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_R2001/annotation318134/range318134/1

https://synbiohub.org/public/igem/BBa_R2001/annotation318134/range318134/1
type: Range
persistent identity: range318134
display id: range318134
version: 1
direction: direction/forward
top level: Zif23
start: 45
end: 45
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation318132
display id: annotation318132
version: 1
top level: Zif23
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R2001/annotation318132/range318132/1

https://synbiohub.org/public/igem/BBa_R2001/annotation318132/range318132/1
type: Range
persistent identity: range318132
display id: range318132
version: 1
direction: direction/forward
top level: Zif23
start: 10
end: 15
orientation: inline

eGFP
type: ComponentDefinition
persistent identity: BBa K678006
display id: BBa_K678006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: eGFP for mammalian cells
creator: DTU-Denmark-2
created: 2011-09-03T11:00:00Z
modified: 2015-05-08T01:13:04Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _882_
m user id: 0
owner id: 8341
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: enhanced green fluorescent protein for use in mammalian cells
mutable notes: Compatible with the Plug'n'Play assembly standard
mutable provenance: plasmid pEGFP-1 from Clontech
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: eGFP
sequence annotation: Linker 2
sequence annotation: Kozak
sequence: https://synbiohub.org/public/igem/BBa_K678006_sequence/1

eGFP
type: SequenceAnnotation
persistent identity: annotation2127349
display id: annotation2127349
version: 1
top level: eGFP
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K678006/annotation2127349/range2127349/1

https://synbiohub.org/public/igem/BBa_K678006/annotation2127349/range2127349/1
type: Range
persistent identity: range2127349
display id: range2127349
version: 1
direction: direction/forward
top level: eGFP
start: 16
end: 735
orientation: inline

Linker 2
type: SequenceAnnotation
persistent identity: annotation2127348
display id: annotation2127348
version: 1
top level: eGFP
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K678006/annotation2127348/range2127348/1

https://synbiohub.org/public/igem/BBa_K678006/annotation2127348/range2127348/1
type: Range
persistent identity: range2127348
display id: range2127348
version: 1
direction: direction/forward
top level: eGFP
start: 1
end: 8
orientation: inline

Kozak
type: SequenceAnnotation
persistent identity: annotation2127774
display id: annotation2127774
version: 1
top level: eGFP
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K678006/annotation2127774/range2127774/1

https://synbiohub.org/public/igem/BBa_K678006/annotation2127774/range2127774/1
type: Range
persistent identity: range2127774
display id: range2127774
version: 1
direction: direction/forward
top level: eGFP
start: 9
end: 19
orientation: inline

BBa_B0013
type: ComponentDefinition
persistent identity: BBa B0013
display id: BBa_B0013
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TE from coliphage T7 (+/-)
creator: Reshma Shetty
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Strong transcriptional terminator consisting of a 20 bp stem-loop that has been engineered to be bidirectional.
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Subsequently mutations were introduced to make the terminator bi-directional (i.e., AAAAAA insertion on 5' side of the stem loop). Additional mutations were introduced on the 3' side of the stem loop to increase the number of T's and eliminate any promoter -10 site that might be present to avoid initiation of transcription of whatever is downstream. Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 V01146 .
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0013
sequence annotation: polya
sequence annotation: T7 TE
sequence annotation: ''
sequence annotation: C
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0013_sequence/1

BBa_B0013
type: SequenceAnnotation
persistent identity: annotation7021
display id: annotation7021
version: 1
top level: BBa_B0013
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_B0013/annotation7021/range7021/1

https://synbiohub.org/public/igem/BBa_B0013/annotation7021/range7021/1
type: Range
persistent identity: range7021
display id: range7021
version: 1
direction: direction/forward
top level: BBa_B0013
start: 1
end: 47
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1698
display id: annotation1698
version: 1
top level: BBa_B0013
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_B0013/annotation1698/range1698/1

https://synbiohub.org/public/igem/BBa_B0013/annotation1698/range1698/1
type: Range
persistent identity: range1698
display id: range1698
version: 1
direction: direction/forward
top level: BBa_B0013
start: 34
end: 47
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1691
display id: annotation1691
version: 1
top level: BBa_B0013
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_B0013/annotation1691/range1691/1

https://synbiohub.org/public/igem/BBa_B0013/annotation1691/range1691/1
type: Range
persistent identity: range1691
display id: range1691
version: 1
direction: direction/forward
top level: BBa_B0013
start: 14
end: 33
orientation: inline

''
type: SequenceAnnotation
persistent identity: annotation1697
display id: annotation1697
version: 1
top level: BBa_B0013
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_B0013/annotation1697/range1697/1

https://synbiohub.org/public/igem/BBa_B0013/annotation1697/range1697/1
type: Range
persistent identity: range1697
display id: range1697
version: 1
direction: direction/forward
top level: BBa_B0013
start: 1
end: 6
orientation: inline

C
type: SequenceAnnotation
persistent identity: annotation1696
display id: annotation1696
version: 1
top level: BBa_B0013
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_B0013/annotation1696/range1696/1

https://synbiohub.org/public/igem/BBa_B0013/annotation1696/range1696/1
type: Range
persistent identity: range1696
display id: range1696
version: 1
direction: direction/forward
top level: BBa_B0013
start: 10
end: 10
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1692
display id: annotation1692
version: 1
top level: BBa_B0013
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_B0013/annotation1692/range1692/1

https://synbiohub.org/public/igem/BBa_B0013/annotation1692/range1692/1
type: Range
persistent identity: range1692
display id: range1692
version: 1
direction: direction/forward
top level: BBa_B0013
start: 40
end: 40
orientation: inline

C
type: SequenceAnnotation
persistent identity: annotation1695
display id: annotation1695
version: 1
top level: BBa_B0013
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_B0013/annotation1695/range1695/1

https://synbiohub.org/public/igem/BBa_B0013/annotation1695/range1695/1
type: Range
persistent identity: range1695
display id: range1695
version: 1
direction: direction/forward
top level: BBa_B0013
start: 37
end: 37
orientation: inline

BBa_K1486003
type: ComponentDefinition
persistent identity: BBa K1486003
display id: BBa_K1486003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Flexible linker 2x (GGGS)
creator: iGem EPFL 2014
created: 2014-08-11T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Flexible linker used to fuse two proteins.
mutable notes: Designed considering the flexibility and separation needed between our fused proteins.
mutable provenance: Synthetic.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence: https://synbiohub.org/public/igem/BBa_K1486003_sequence/1

BBa_J05217
type: ComponentDefinition
persistent identity: BBa J05217
display id: BBa_J05217
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Regulator for R2-YAP7
creator: Alexander Roth
created: 2005-09-07T11:00:00Z
modified: 2015-08-31T04:08:15Z
discontinued: false
dominant: false
group u list: _34_
m user id: 0
owner id: 412
owning group id: 34
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: binding sites for R3-ATF6 and R4-cMaf
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J05217_sequence/1

BBa_K165041
type: ComponentDefinition
persistent identity: BBa K165041
display id: BBa_K165041
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Zif268-HIV binding sites + TEF constitutive yeast promoter
creator: John Szymanski
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:10:56Z
discontinued: false
dominant: false
experience: experience/None
group u list: _267_
m user id: 0
owner id: 2512
owning group id: 58
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: -
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
component: BBa_K165011
component: BBa_K165037
sequence annotation: BBa_K165037
sequence annotation: BBa_K165011
sequence: https://synbiohub.org/public/igem/BBa_K165041_sequence/1

BBa_K165011
type: Component
persistent identity: component1996557
display id: component1996557
version: 1
top level: BBa_K165041
access: public
definition: https://synbiohub.org/public/igem/BBa_K165011/1

BBa_K165037
type: Component
persistent identity: component1996558
display id: component1996558
version: 1
top level: BBa_K165041
access: public
definition: pTEF

BBa_K165037
type: SequenceAnnotation
persistent identity: annotation1996558
display id: annotation1996558
version: 1
top level: BBa_K165041
location: https://synbiohub.org/public/igem/BBa_K165041/annotation1996558/range1996558/1
component: BBa_K165037

https://synbiohub.org/public/igem/BBa_K165041/annotation1996558/range1996558/1
type: Range
persistent identity: range1996558
display id: range1996558
version: 1
direction: direction/reverse
top level: BBa_K165041
start: 55
end: 457
orientation: inline

BBa_K165011
type: SequenceAnnotation
persistent identity: annotation1996557
display id: annotation1996557
version: 1
top level: BBa_K165041
location: https://synbiohub.org/public/igem/BBa_K165041/annotation1996557/range1996557/1
component: BBa_K165011

https://synbiohub.org/public/igem/BBa_K165041/annotation1996557/range1996557/1
type: Range
persistent identity: range1996557
display id: range1996557
version: 1
direction: direction/reverse
top level: BBa_K165041
start: 1
end: 46
orientation: inline

BBa_K180013
type: ComponentDefinition
persistent identity: BBa K180013
display id: BBa_K180013
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Rock-paper-scissors - Rock secondary plasmid [rhl pR, LacI generator]
creator: Richard Wilson, Stephanie Chambers
created: 2009-10-18T11:00:00Z
modified: 2015-05-08T01:11:06Z
discontinued: false
dominant: false
experience: experience/None
group u list: _278_
m user id: 0
owner id: 4082
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: iGEM2009 Team:Southampton Game of Life secondary plasmid.
mutable notes: Regulated expression of LacI (C0012) by Rhl pR (R0071).
mutable provenance: See relevant basic biobrick parts (R0071, B0034, C0012, B0015)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_R0071
component: BBa_B0034
component: BBa_B0015
component: BBa_C0012
sequence annotation: BBa_B0034
sequence annotation: BBa_R0071
sequence annotation: BBa_B0015
sequence annotation: BBa_C0012
sequence: https://synbiohub.org/public/igem/BBa_K180013_sequence/1

BBa_R0071
type: Component
persistent identity: component2256419
display id: component2256419
version: 1
top level: BBa_K180013
access: public
definition: RhlR+C4

BBa_B0034
type: Component
persistent identity: component2256421
display id: component2256421
version: 1
top level: BBa_K180013
access: public
definition: BBa_B0034

BBa_B0015
type: Component
persistent identity: component2256432
display id: component2256432
version: 1
top level: BBa_K180013
access: public
definition: BBa_B0015

BBa_C0012
type: Component
persistent identity: component2256423
display id: component2256423
version: 1
top level: BBa_K180013
access: public
definition: https://synbiohub.org/public/igem/BBa_C0012/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2256421
display id: annotation2256421
version: 1
top level: BBa_K180013
location: https://synbiohub.org/public/igem/BBa_K180013/annotation2256421/range2256421/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K180013/annotation2256421/range2256421/1
type: Range
persistent identity: range2256421
display id: range2256421
version: 1
direction: direction/reverse
top level: BBa_K180013
start: 62
end: 73
orientation: inline

BBa_R0071
type: SequenceAnnotation
persistent identity: annotation2256419
display id: annotation2256419
version: 1
top level: BBa_K180013
location: https://synbiohub.org/public/igem/BBa_K180013/annotation2256419/range2256419/1
component: BBa_R0071

https://synbiohub.org/public/igem/BBa_K180013/annotation2256419/range2256419/1
type: Range
persistent identity: range2256419
display id: range2256419
version: 1
direction: direction/reverse
top level: BBa_K180013
start: 1
end: 53
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2256432
display id: annotation2256432
version: 1
top level: BBa_K180013
location: https://synbiohub.org/public/igem/BBa_K180013/annotation2256432/range2256432/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K180013/annotation2256432/range2256432/1
type: Range
persistent identity: range2256432
display id: range2256432
version: 1
direction: direction/reverse
top level: BBa_K180013
start: 1241
end: 1369
orientation: inline

BBa_C0012
type: SequenceAnnotation
persistent identity: annotation2256423
display id: annotation2256423
version: 1
top level: BBa_K180013
location: https://synbiohub.org/public/igem/BBa_K180013/annotation2256423/range2256423/1
component: BBa_C0012

https://synbiohub.org/public/igem/BBa_K180013/annotation2256423/range2256423/1
type: Range
persistent identity: range2256423
display id: range2256423
version: 1
direction: direction/forward
top level: BBa_K180013
start: 80
end: 1207
orientation: inline

BBa_I742150
type: ComponentDefinition
persistent identity: BBa I742150
display id: BBa_I742150
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: crtE native rbs
creator: Chris French
created: 2007-10-23T11:00:00Z
modified: 2015-08-31T04:08:03Z
discontinued: false
dominant: false
experience: experience/None
group u list: _123_
m user id: 0
owner id: 837
owning group id: 163
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is the native ribosome binding site of crtE (geranylgeranyl pyrophosphate synthase) from Pantoea ananatis (formerly Erwinia uredovora) DSMZ 30080 (ATCC 19321) Accession: D90087.
mutable notes: This is a virtual part to be added, blunt ended, to the coding sequence.
mutable provenance: Pantoea ananatis (formerly Erwinia uredovora) DSMZ 30080 (ATCC 19321) Accession: D90087.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_I742150_sequence/1

Pnot(O22)
type: ComponentDefinition
persistent identity: BBa I732202
display id: BBa_I732202
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: NOT Gate Promoter Family Member (D001O22)
creator: Zhan Jian
created: 2007-08-11T11:00:00Z
modified: 2015-08-31T04:07:57Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732202_sequence/1

BBa_I714924
type: ComponentDefinition
persistent identity: BBa I714924
display id: BBa_I714924
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RecA_DlexO_DLacO1
creator: Chunbo Lou
created: 2007-10-21T11:00:00Z
modified: 2015-08-31T04:07:48Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _142_
m user id: 0
owner id: 1911
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: a NOR gate
mutable notes: NOR gat
mutable provenance: synthetic
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -10
sequence annotation: SDY_EGFP
sequence annotation: -35
sequence annotation: LexA
sequence annotation: LacO1
sequence: https://synbiohub.org/public/igem/BBa_I714924_sequence/1

-10
type: SequenceAnnotation
persistent identity: annotation1951926
display id: annotation1951926
version: 1
top level: BBa_I714924
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I714924/annotation1951926/range1951926/1

https://synbiohub.org/public/igem/BBa_I714924/annotation1951926/range1951926/1
type: Range
persistent identity: range1951926
display id: range1951926
version: 1
direction: direction/forward
top level: BBa_I714924
start: 81
end: 86
orientation: inline

SDY_EGFP
type: SequenceAnnotation
persistent identity: annotation1956422
display id: annotation1956422
version: 1
top level: BBa_I714924
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_I714924/annotation1956422/range1956422/1

https://synbiohub.org/public/igem/BBa_I714924/annotation1956422/range1956422/1
type: Range
persistent identity: range1956422
display id: range1956422
version: 1
direction: direction/forward
top level: BBa_I714924
start: 121
end: 862
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1951925
display id: annotation1951925
version: 1
top level: BBa_I714924
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I714924/annotation1951925/range1951925/1

https://synbiohub.org/public/igem/BBa_I714924/annotation1951925/range1951925/1
type: Range
persistent identity: range1951925
display id: range1951925
version: 1
direction: direction/forward
top level: BBa_I714924
start: 59
end: 64
orientation: inline

LexA
type: SequenceAnnotation
persistent identity: annotation1951922
display id: annotation1951922
version: 1
top level: BBa_I714924
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I714924/annotation1951922/range1951922/1

https://synbiohub.org/public/igem/BBa_I714924/annotation1951922/range1951922/1
type: Range
persistent identity: range1951922
display id: range1951922
version: 1
direction: direction/forward
top level: BBa_I714924
start: 65
end: 80
orientation: inline

LacO1
type: SequenceAnnotation
persistent identity: annotation1951924
display id: annotation1951924
version: 1
top level: BBa_I714924
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I714924/annotation1951924/range1951924/1

https://synbiohub.org/public/igem/BBa_I714924/annotation1951924/range1951924/1
type: Range
persistent identity: range1951924
display id: range1951924
version: 1
direction: direction/forward
top level: BBa_I714924
start: 93
end: 112
orientation: inline

LacO1
type: SequenceAnnotation
persistent identity: annotation1951921
display id: annotation1951921
version: 1
top level: BBa_I714924
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I714924/annotation1951921/range1951921/1

https://synbiohub.org/public/igem/BBa_I714924/annotation1951921/range1951921/1
type: Range
persistent identity: range1951921
display id: range1951921
version: 1
direction: direction/forward
top level: BBa_I714924
start: 1
end: 21
orientation: inline

LexA
type: SequenceAnnotation
persistent identity: annotation1956155
display id: annotation1956155
version: 1
top level: BBa_I714924
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I714924/annotation1956155/range1956155/1

https://synbiohub.org/public/igem/BBa_I714924/annotation1956155/range1956155/1
type: Range
persistent identity: range1956155
display id: range1956155
version: 1
direction: direction/forward
top level: BBa_I714924
start: 35
end: 55
orientation: inline

BBa_J23108
type: ComponentDefinition
persistent identity: BBa J23108
display id: BBa_J23108
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: constitutive promoter family member
creator: John Anderson
created: 2006-08-16T11:00:00Z
modified: 2015-08-31T04:08:40Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: true
mutable description: Later
mutable notes: N/A
mutable provenance: Later
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J23108_sequence/1

BBa_K1895007
type: ComponentDefinition
persistent identity: BBa K1895007
display id: BBa_K1895007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Sequence for sigma 32 transcription factor
creator: Jake Burton
created: 2016-10-17T11:00:00Z
modified: 2016-10-18T02:13:40Z
discontinued: false
dominant: false
group u list: _2361_
m user id: 29859
owner id: 29859
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This part is a correction to [http://parts.igem.org/Part:BBa_M36888 BBa_M36888] which has an illegal PstI site which makes it incompatible with BioBricks / RFC10. iGEM Teams can use this alternative sequence which contains none of the BioBrick restriction enzyme sites. Amino acid No. 202, Glysine has been mutated from CAG to CAA to remove the PstI site. The remainder of the sequence remains unchanged.
mutable notes: This part was checked for BioBrick restriction enzyme sites which would otherwise make it unsuitable for use by iGEM teams.
mutable provenance: This part is a correction to [http://parts.igem.org/Part:BBa_M36888 BBa_M36888].
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1895007_sequence/1

BBa_I732452
type: ComponentDefinition
persistent identity: BBa I732452
display id: BBa_I732452
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Family Member (U073O26+D062O61)
creator: Zhan Jian
created: 2007-10-16T11:00:00Z
modified: 2015-08-31T04:07:58Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732452_sequence/1

P0310
type: ComponentDefinition
persistent identity: BBa J63005
display id: BBa_J63005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: yeast ADH1 promoter
creator: Caroline Ajo-Franklin
created: 2006-10-10T11:00:00Z
modified: 2015-08-31T01:56:26Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _97_
m user id: 0
owner id: 545
owning group id: 97
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: ADH1 promoter from S. cerevisiae
mutable notes: no changes to genomic sequence
mutable provenance: genomic DNA of ADH1 from S. cerevisiae
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: Constitutive promoter
sequence annotation: TATA Box
sequence: https://synbiohub.org/public/igem/BBa_J63005_sequence/1

Constitutive promoter
type: SequenceAnnotation
persistent identity: annotation1902744
display id: annotation1902744
version: 1
top level: P0310
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_J63005/annotation1902744/range1902744/1

https://synbiohub.org/public/igem/BBa_J63005/annotation1902744/range1902744/1
type: Range
persistent identity: range1902744
display id: range1902744
version: 1
direction: direction/forward
top level: P0310
start: 1
end: 1445
orientation: inline

TATA Box
type: SequenceAnnotation
persistent identity: annotation1902745
display id: annotation1902745
version: 1
top level: P0310
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_J63005/annotation1902745/range1902745/1

https://synbiohub.org/public/igem/BBa_J63005/annotation1902745/range1902745/1
type: Range
persistent identity: range1902745
display id: range1902745
version: 1
direction: direction/forward
top level: P0310
start: 1348
end: 1354
orientation: inline

pRecA
type: ComponentDefinition
persistent identity: BBa K093000
display id: BBa_K093000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pRecA with LexA binding site
creator: Julian Wiegelmann, Danielle Nash
created: 2008-10-25T11:00:00Z
modified: 2015-05-08T01:08:40Z
discontinued: false
dominant: false
experience: experience/None
group u list: _247_
m user id: 0
owner id: 3630
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This is a pRecA promoter with LexA binding sites. LexA is cleaved by RecA when RecA is upregulated due to induction of the SOS response triggered by single stranded DNA. For this part to work it must be in a RecA+ strain of E. coli such as TG1
mutable notes: How far past the start site of transcription do you need to go in order for the promoter to drive transcription? The promoter has been confirmed to drive transcription in a regular DH5alpha strain, so it the answer to the question is, 3 bp before the suffix, maybe less. The LexA binding site is in the core region of the promoter (between the -35 and -10 sigma 70 binding sites). According to Robert Sidney Cox III and colleagues in their paper "Programming ene expression with combinatorial promoters, the core region is the most effective region for binding of repressor molecules.
mutable provenance: synthetic. Oligonucleotides were designed to form the double stranded molecule with sticky ends for direct ligation into a biobrick vector.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -35
sequence annotation: LexA binding
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_K093000_sequence/1

-35
type: SequenceAnnotation
persistent identity: annotation1989749
display id: annotation1989749
version: 1
top level: pRecA
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K093000/annotation1989749/range1989749/1

https://synbiohub.org/public/igem/BBa_K093000/annotation1989749/range1989749/1
type: Range
persistent identity: range1989749
display id: range1989749
version: 1
direction: direction/forward
top level: pRecA
start: 11
end: 17
orientation: inline

LexA binding
type: SequenceAnnotation
persistent identity: annotation1989779
display id: annotation1989779
version: 1
top level: pRecA
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K093000/annotation1989779/range1989779/1

https://synbiohub.org/public/igem/BBa_K093000/annotation1989779/range1989779/1
type: Range
persistent identity: range1989779
display id: range1989779
version: 1
direction: direction/forward
top level: pRecA
start: 15
end: 35
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1989775
display id: annotation1989775
version: 1
top level: pRecA
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K093000/annotation1989775/range1989775/1

https://synbiohub.org/public/igem/BBa_K093000/annotation1989775/range1989775/1
type: Range
persistent identity: range1989775
display id: range1989775
version: 1
direction: direction/forward
top level: pRecA
start: 30
end: 39
orientation: inline

BBa_K112118
type: ComponentDefinition
persistent identity: BBa K112118
display id: BBa_K112118
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: rrnB P1 promoter
creator: Bing Xia
created: 2008-10-20T11:00:00Z
modified: 2015-05-08T01:09:14Z
discontinued: false
dominant: false
group u list: _224_
m user id: 0
owner id: 2998
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: ---- This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at: [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
mutable provenance: PCR mv028/mv029 on rrnB native sequence (721bp, EcoRI/BamHI/DpnI) Sub into pBca1256 (EcoRI/BamHI, 2472+910, L) Product is pBca1256-K112118 ----------------------------------------------- mv028 Forward Biobricking of rrnB P1 promoter cgataGAATTCatgAGATCTatgcactaaaacgtatcctacgcccg mv029 Reverse Biobricking of rrnB P1 promoter cgttaGGATCCcgccttcccgctacagagtcaagc
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K112118_sequence/1

eGFP-PTS1
type: ComponentDefinition
persistent identity: BBa K678002
display id: BBa_K678002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: eGFP-PTS1 for mammalian cells
creator: DTU-Denmark-2
created: 2011-09-01T11:00:00Z
modified: 2015-05-08T01:13:04Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _882_
m user id: 0
owner id: 8341
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: Vector expressing eGFP from the CMV promoter with the BGH polyA tail and with a hygromycin resistance cassette
mutable notes: Compatible with the Plug'n'Play with DNA assembly system.
mutable provenance: Source to be announced
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
sequence annotation: PTS1
sequence annotation: Hygromycin cassette
sequence annotation: CMV
sequence annotation: BGH pA
sequence annotation: Linker 6
sequence annotation: Linker 5
sequence annotation: Linker 4
sequence annotation: Linker 2
sequence annotation: eGFP
sequence annotation: Linker 1
sequence annotation: Kozak
sequence: https://synbiohub.org/public/igem/BBa_K678002_sequence/1

PTS1
type: SequenceAnnotation
persistent identity: annotation2126936
display id: annotation2126936
version: 1
top level: eGFP-PTS1
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K678002/annotation2126936/range2126936/1

https://synbiohub.org/public/igem/BBa_K678002/annotation2126936/range2126936/1
type: Range
persistent identity: range2126936
display id: range2126936
version: 1
direction: direction/forward
top level: eGFP-PTS1
start: 1395
end: 1406
orientation: inline

Hygromycin cassette
type: SequenceAnnotation
persistent identity: annotation2126940
display id: annotation2126940
version: 1
top level: eGFP-PTS1
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K678002/annotation2126940/range2126940/1

https://synbiohub.org/public/igem/BBa_K678002/annotation2126940/range2126940/1
type: Range
persistent identity: range2126940
display id: range2126940
version: 1
direction: direction/forward
top level: eGFP-PTS1
start: 1648
end: 3398
orientation: inline

CMV
type: SequenceAnnotation
persistent identity: annotation2126913
display id: annotation2126913
version: 1
top level: eGFP-PTS1
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K678002/annotation2126913/range2126913/1

https://synbiohub.org/public/igem/BBa_K678002/annotation2126913/range2126913/1
type: Range
persistent identity: range2126913
display id: range2126913
version: 1
direction: direction/forward
top level: eGFP-PTS1
start: 9
end: 662
orientation: inline

BGH pA
type: SequenceAnnotation
persistent identity: annotation2126938
display id: annotation2126938
version: 1
top level: eGFP-PTS1
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_K678002/annotation2126938/range2126938/1

https://synbiohub.org/public/igem/BBa_K678002/annotation2126938/range2126938/1
type: Range
persistent identity: range2126938
display id: range2126938
version: 1
direction: direction/forward
top level: eGFP-PTS1
start: 1415
end: 1639
orientation: inline

Linker 6
type: SequenceAnnotation
persistent identity: annotation2126941
display id: annotation2126941
version: 1
top level: eGFP-PTS1
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K678002/annotation2126941/range2126941/1

https://synbiohub.org/public/igem/BBa_K678002/annotation2126941/range2126941/1
type: Range
persistent identity: range2126941
display id: range2126941
version: 1
direction: direction/forward
top level: eGFP-PTS1
start: 3399
end: 3406
orientation: inline

Linker 5
type: SequenceAnnotation
persistent identity: annotation2126939
display id: annotation2126939
version: 1
top level: eGFP-PTS1
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K678002/annotation2126939/range2126939/1

https://synbiohub.org/public/igem/BBa_K678002/annotation2126939/range2126939/1
type: Range
persistent identity: range2126939
display id: range2126939
version: 1
direction: direction/forward
top level: eGFP-PTS1
start: 1640
end: 1647
orientation: inline

Linker 4
type: SequenceAnnotation
persistent identity: annotation2126937
display id: annotation2126937
version: 1
top level: eGFP-PTS1
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K678002/annotation2126937/range2126937/1

https://synbiohub.org/public/igem/BBa_K678002/annotation2126937/range2126937/1
type: Range
persistent identity: range2126937
display id: range2126937
version: 1
direction: direction/forward
top level: eGFP-PTS1
start: 1407
end: 1414
orientation: inline

Linker 2
type: SequenceAnnotation
persistent identity: annotation2126914
display id: annotation2126914
version: 1
top level: eGFP-PTS1
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K678002/annotation2126914/range2126914/1

https://synbiohub.org/public/igem/BBa_K678002/annotation2126914/range2126914/1
type: Range
persistent identity: range2126914
display id: range2126914
version: 1
direction: direction/forward
top level: eGFP-PTS1
start: 663
end: 671
orientation: inline

eGFP
type: SequenceAnnotation
persistent identity: annotation2126934
display id: annotation2126934
version: 1
top level: eGFP-PTS1
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K678002/annotation2126934/range2126934/1

https://synbiohub.org/public/igem/BBa_K678002/annotation2126934/range2126934/1
type: Range
persistent identity: range2126934
display id: range2126934
version: 1
direction: direction/forward
top level: eGFP-PTS1
start: 679
end: 1394
orientation: inline

Linker 1
type: SequenceAnnotation
persistent identity: annotation2126912
display id: annotation2126912
version: 1
top level: eGFP-PTS1
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K678002/annotation2126912/range2126912/1

https://synbiohub.org/public/igem/BBa_K678002/annotation2126912/range2126912/1
type: Range
persistent identity: range2126912
display id: range2126912
version: 1
direction: direction/forward
top level: eGFP-PTS1
start: 1
end: 8
orientation: inline

Kozak
type: SequenceAnnotation
persistent identity: annotation2126935
display id: annotation2126935
version: 1
top level: eGFP-PTS1
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K678002/annotation2126935/range2126935/1

https://synbiohub.org/public/igem/BBa_K678002/annotation2126935/range2126935/1
type: Range
persistent identity: range2126935
display id: range2126935
version: 1
direction: direction/forward
top level: eGFP-PTS1
start: 672
end: 682
orientation: inline

BBa_J61110
type: ComponentDefinition
persistent identity: BBa J61110
display id: BBa_J61110
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Ribosome Binding Site Family Member
creator: John Anderson
created: 2007-04-23T11:00:00Z
modified: 2015-08-31T02:03:00Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: false
mutable description: {{JCA_Arkin_RBSFamily}}
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J61110_sequence/1

BBa_K256020
type: ComponentDefinition
persistent identity: BBa K256020
display id: BBa_K256020
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: J23119:HO1
creator: iGEM09_NTU-Singapore
created: 2009-10-18T11:00:00Z
modified: 2015-05-08T01:11:41Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _359_
m user id: 0
owner id: 4864
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: Generator of Heme Oxygenase (HO1)
mutable notes: --
mutable provenance: --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_J23119
component: BBa_B0015
component: BBa_I15008
component: BBa_B0034
sequence annotation: BBa_B0034
sequence annotation: BBa_B0015
sequence annotation: BBa_I15008
sequence annotation: BBa_J23119
sequence: https://synbiohub.org/public/igem/BBa_K256020_sequence/1

BBa_J23119
type: Component
persistent identity: component2256388
display id: component2256388
version: 1
top level: BBa_K256020
access: public
definition: BBa_J23119

BBa_B0015
type: Component
persistent identity: component2256399
display id: component2256399
version: 1
top level: BBa_K256020
access: public
definition: BBa_B0015

BBa_I15008
type: Component
persistent identity: component2256392
display id: component2256392
version: 1
top level: BBa_K256020
access: public
definition: https://synbiohub.org/public/igem/BBa_I15008/1

BBa_B0034
type: Component
persistent identity: component2256390
display id: component2256390
version: 1
top level: BBa_K256020
access: public
definition: BBa_B0034

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2256390
display id: annotation2256390
version: 1
top level: BBa_K256020
location: https://synbiohub.org/public/igem/BBa_K256020/annotation2256390/range2256390/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K256020/annotation2256390/range2256390/1
type: Range
persistent identity: range2256390
display id: range2256390
version: 1
direction: direction/reverse
top level: BBa_K256020
start: 44
end: 55
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2256399
display id: annotation2256399
version: 1
top level: BBa_K256020
location: https://synbiohub.org/public/igem/BBa_K256020/annotation2256399/range2256399/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K256020/annotation2256399/range2256399/1
type: Range
persistent identity: range2256399
display id: range2256399
version: 1
direction: direction/reverse
top level: BBa_K256020
start: 821
end: 949
orientation: inline

BBa_I15008
type: SequenceAnnotation
persistent identity: annotation2256392
display id: annotation2256392
version: 1
top level: BBa_K256020
location: https://synbiohub.org/public/igem/BBa_K256020/annotation2256392/range2256392/1
component: BBa_I15008

https://synbiohub.org/public/igem/BBa_K256020/annotation2256392/range2256392/1
type: Range
persistent identity: range2256392
display id: range2256392
version: 1
direction: direction/reverse
top level: BBa_K256020
start: 62
end: 812
orientation: inline

BBa_J23119
type: SequenceAnnotation
persistent identity: annotation2256388
display id: annotation2256388
version: 1
top level: BBa_K256020
location: https://synbiohub.org/public/igem/BBa_K256020/annotation2256388/range2256388/1
component: BBa_J23119

https://synbiohub.org/public/igem/BBa_K256020/annotation2256388/range2256388/1
type: Range
persistent identity: range2256388
display id: range2256388
version: 1
direction: direction/reverse
top level: BBa_K256020
start: 1
end: 35
orientation: inline

BBa_J23103
type: ComponentDefinition
persistent identity: BBa J23103
display id: BBa_J23103
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: constitutive promoter family member
creator: John Anderson
created: 2006-08-03T11:00:00Z
modified: 2015-08-31T04:08:40Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: replace later
mutable notes: N/A
mutable provenance: isolated from library of promoters
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J23103_sequence/1

BBa_K1480002
type: ComponentDefinition
persistent identity: BBa K1480002
display id: BBa_K1480002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pTet + RBS + mRFP1 + Transcriptional terminator
creator: Jesus Gilberto Rodriguez Ceja
created: 2014-10-07T11:00:00Z
modified: 2015-05-08T01:10:40Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1860_
m user id: 0
owner id: 12878
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part codifies for a red fluorescent protein (BBa_K081014). It??s regulated by a TetR repressible promoter (R0040).
mutable notes: Assembled using assembly 10.
mutable provenance: mRFP: Monomeric red fluorescent protein engineered to enhance photoresistance (BBa_E1010). It was used the part BBa_K081014. ptet: Based in the TetR repressible promoter of operon for resistance against tetracycline (BBa_R0040).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_E1010
component: BBa_R0040
component: BBa_B1006
component: BBa_B0030
sequence annotation: BBa_E1010
sequence annotation: BBa_B0030
sequence annotation: BBa_R0040
sequence annotation: BBa_B1006
sequence: https://synbiohub.org/public/igem/BBa_K1480002_sequence/1

BBa_E1010
type: Component
persistent identity: component2406226
display id: component2406226
version: 1
top level: BBa_K1480002
access: public
definition: https://synbiohub.org/public/igem/BBa_E1010/1

BBa_R0040
type: Component
persistent identity: component2406216
display id: component2406216
version: 1
top level: BBa_K1480002
access: public
definition: p(tetR)

BBa_B1006
type: Component
persistent identity: component2406231
display id: component2406231
version: 1
top level: BBa_K1480002
access: public
definition: BBa_B1006

BBa_B0030
type: Component
persistent identity: component2406222
display id: component2406222
version: 1
top level: BBa_K1480002
access: public
definition: BBa_B0030

BBa_E1010
type: SequenceAnnotation
persistent identity: annotation2406226
display id: annotation2406226
version: 1
top level: BBa_K1480002
location: https://synbiohub.org/public/igem/BBa_K1480002/annotation2406226/range2406226/1
component: BBa_E1010

https://synbiohub.org/public/igem/BBa_K1480002/annotation2406226/range2406226/1
type: Range
persistent identity: range2406226
display id: range2406226
version: 1
direction: direction/reverse
top level: BBa_K1480002
start: 84
end: 789
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation2406222
display id: annotation2406222
version: 1
top level: BBa_K1480002
location: https://synbiohub.org/public/igem/BBa_K1480002/annotation2406222/range2406222/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_K1480002/annotation2406222/range2406222/1
type: Range
persistent identity: range2406222
display id: range2406222
version: 1
direction: direction/forward
top level: BBa_K1480002
start: 63
end: 77
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation2406216
display id: annotation2406216
version: 1
top level: BBa_K1480002
location: https://synbiohub.org/public/igem/BBa_K1480002/annotation2406216/range2406216/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_K1480002/annotation2406216/range2406216/1
type: Range
persistent identity: range2406216
display id: range2406216
version: 1
direction: direction/forward
top level: BBa_K1480002
start: 1
end: 54
orientation: inline

BBa_B1006
type: SequenceAnnotation
persistent identity: annotation2406231
display id: annotation2406231
version: 1
top level: BBa_K1480002
location: https://synbiohub.org/public/igem/BBa_K1480002/annotation2406231/range2406231/1
component: BBa_B1006

https://synbiohub.org/public/igem/BBa_K1480002/annotation2406231/range2406231/1
type: Range
persistent identity: range2406231
display id: range2406231
version: 1
direction: direction/reverse
top level: BBa_K1480002
start: 798
end: 836
orientation: inline

U085O11+D0
type: ComponentDefinition
persistent identity: BBa I732424
display id: BBa_I732424
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Family Member (U085O11+D062O22)
creator: Zhan Jian
created: 2007-10-13T11:00:00Z
modified: 2015-08-31T04:07:58Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732424_sequence/1

BBa_K1486040
type: ComponentDefinition
persistent identity: BBa K1486040
display id: BBa_K1486040
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: sfGFPN + CpxR
creator: EPFL iGem team 2014
created: 2014-09-26T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: To edit
mutable notes: To edit
mutable provenance: To edit
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0034
component: BBa_K1486038
component: BBa_K1486037
component: BBa_K1486000
component: BBa_I0500
sequence annotation: BBa_K1486038
sequence annotation: BBa_K1486000
sequence annotation: BBa_K1486037
sequence annotation: BBa_I0500
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K1486040_sequence/1

BBa_B0034
type: Component
persistent identity: component2387187
display id: component2387187
version: 1
top level: BBa_K1486040
access: public
definition: BBa_B0034

BBa_K1486038
type: Component
persistent identity: component2387188
display id: component2387188
version: 1
top level: BBa_K1486040
access: public
definition: BBa_K1486038

BBa_K1486037
type: Component
persistent identity: component2387189
display id: component2387189
version: 1
top level: BBa_K1486040
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486037/1

BBa_K1486000
type: Component
persistent identity: component2387193
display id: component2387193
version: 1
top level: BBa_K1486040
access: public
definition: BBa_K1486000

BBa_I0500
type: Component
persistent identity: component2387185
display id: component2387185
version: 1
top level: BBa_K1486040
access: public
definition: pBad/araC

BBa_K1486038
type: SequenceAnnotation
persistent identity: annotation2387188
display id: annotation2387188
version: 1
top level: BBa_K1486040
location: https://synbiohub.org/public/igem/BBa_K1486040/annotation2387188/range2387188/1
component: BBa_K1486038

https://synbiohub.org/public/igem/BBa_K1486040/annotation2387188/range2387188/1
type: Range
persistent identity: range2387188
display id: range2387188
version: 1
direction: direction/reverse
top level: BBa_K1486040
start: 1223
end: 1864
orientation: inline

BBa_K1486000
type: SequenceAnnotation
persistent identity: annotation2387193
display id: annotation2387193
version: 1
top level: BBa_K1486040
location: https://synbiohub.org/public/igem/BBa_K1486040/annotation2387193/range2387193/1
component: BBa_K1486000

https://synbiohub.org/public/igem/BBa_K1486040/annotation2387193/range2387193/1
type: Range
persistent identity: range2387193
display id: range2387193
version: 1
direction: direction/reverse
top level: BBa_K1486040
start: 1904
end: 2605
orientation: inline

BBa_K1486037
type: SequenceAnnotation
persistent identity: annotation2387189
display id: annotation2387189
version: 1
top level: BBa_K1486040
location: https://synbiohub.org/public/igem/BBa_K1486040/annotation2387189/range2387189/1
component: BBa_K1486037

https://synbiohub.org/public/igem/BBa_K1486040/annotation2387189/range2387189/1
type: Range
persistent identity: range2387189
display id: range2387189
version: 1
direction: direction/reverse
top level: BBa_K1486040
start: 1865
end: 1903
orientation: inline

BBa_I0500
type: SequenceAnnotation
persistent identity: annotation2387185
display id: annotation2387185
version: 1
top level: BBa_K1486040
location: https://synbiohub.org/public/igem/BBa_K1486040/annotation2387185/range2387185/1
component: BBa_I0500

https://synbiohub.org/public/igem/BBa_K1486040/annotation2387185/range2387185/1
type: Range
persistent identity: range2387185
display id: range2387185
version: 1
direction: direction/reverse
top level: BBa_K1486040
start: 1
end: 1210
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2387187
display id: annotation2387187
version: 1
top level: BBa_K1486040
location: https://synbiohub.org/public/igem/BBa_K1486040/annotation2387187/range2387187/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1486040/annotation2387187/range2387187/1
type: Range
persistent identity: range2387187
display id: range2387187
version: 1
direction: direction/reverse
top level: BBa_K1486040
start: 1211
end: 1222
orientation: inline

BBa_K1895003
type: ComponentDefinition
persistent identity: BBa K1895003
display id: BBa_K1895003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: htpG Promoter
creator: iGEM16_Newcastle
created: 2016-09-05T11:00:00Z
modified: 2016-09-06T04:50:25Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2361_
m user id: 29859
owner id: 29859
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This is the promoter for molecular chaperone htpG. It is active during the heat shock response.
mutable notes: [TODO]
mutable provenance: This is a correction to part [http://parts.igem.org/Part:BBa_J45504 BBa_J45504], also the htpG Heat Shock Promoter. The original part contains a fragment of the coding sequence for molecular chaperone htpG (base pairs >287) which can be confirmed by [http://blast.ncbi.nlm.nih.gov/ BLASTing the sequence]. This part contains only the first 287 bases of the original part which consist solely of the htpG promoter. You can see the difference between the two sequences in the image below.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K1895003_sequence/1

BBa_I756018
type: ComponentDefinition
persistent identity: BBa I756018
display id: BBa_I756018
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Lambda Operator in SV-40 intron
creator: Jack
created: 2007-08-15T11:00:00Z
modified: 2015-08-31T04:08:06Z
discontinued: false
dominant: false
group u list: _108_
m user id: 0
owner id: 2067
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Lambda Operator in SV-40 intron
mutable notes: Lambda Operator in SV-40 intron
mutable provenance: Lambda Operator in SV-40 intron
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I756018_sequence/1

BBa_K116201
type: ComponentDefinition
persistent identity: BBa K116201
display id: BBa_K116201
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: ureD promoter from P mirabilis
creator: Yuan-Hao Lee
created: 2008-10-30T12:00:00Z
modified: 2015-05-08T01:09:32Z
discontinued: false
dominant: false
experience: experience/None
group u list: _210_
m user id: 0
owner id: 2716
owning group id: 9
sample status: No part sequence
status: status/Planning
bookmark: false
mutable description: An 328 bp promoter region, which can be assembled with urea binding proteins to activate the downstream gene expression.
mutable notes: pUreD is a urea responsive element that should be turned on by the complex of UreR and urea.
mutable provenance: This is a component of urea sensor cloned from Proteus mirabilis.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory

U073O11+D0
type: ComponentDefinition
persistent identity: BBa I732429
display id: BBa_I732429
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Family Member (U073O11+D050O22)
creator: Zhan Jian
created: 2007-10-13T11:00:00Z
modified: 2015-08-31T04:07:58Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732429_sequence/1

Pnot(O47)
type: ComponentDefinition
persistent identity: BBa I732278
display id: BBa_I732278
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Family Member with Hybrid Operator (D001O47)
creator: Ma Rui
created: 2007-10-13T11:00:00Z
modified: 2015-08-31T04:07:57Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732278_sequence/1

BBa_K1486000
type: ComponentDefinition
persistent identity: BBa K1486000
display id: BBa_K1486000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CpxR coding region
creator: iGem EPFL 2014
created: 2014-08-11T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: CpxR is a transciption factor that dimerizes and becomes active when phosphorylated by the transmembrane histidine kinase CpxA. CpxA in turn becomes active when the bacterial cell is submitted to different kinds of shock (pH, temperature, pressure, protein misfolding in the inter-membrane space...).
mutable notes: When making composite parts with CpxR that are supposed to dimerize, we had to take care about the positioning of the other elements regarding the parallel dimerization of CpxR.
mutable provenance: The part has been extracted from the E coli genome.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: START
sequence annotation: STOP
sequence annotation: CpxR
sequence: https://synbiohub.org/public/igem/BBa_K1486000_sequence/1

START
type: SequenceAnnotation
persistent identity: annotation2382022
display id: annotation2382022
version: 1
top level: BBa_K1486000
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1486000/annotation2382022/range2382022/1

https://synbiohub.org/public/igem/BBa_K1486000/annotation2382022/range2382022/1
type: Range
persistent identity: range2382022
display id: range2382022
version: 1
direction: direction/forward
top level: BBa_K1486000
start: 1
end: 3
orientation: inline

STOP
type: SequenceAnnotation
persistent identity: annotation2382023
display id: annotation2382023
version: 1
top level: BBa_K1486000
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1486000/annotation2382023/range2382023/1

https://synbiohub.org/public/igem/BBa_K1486000/annotation2382023/range2382023/1
type: Range
persistent identity: range2382023
display id: range2382023
version: 1
direction: direction/forward
top level: BBa_K1486000
start: 699
end: 702
orientation: inline

CpxR
type: SequenceAnnotation
persistent identity: annotation2382021
display id: annotation2382021
version: 1
top level: BBa_K1486000
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1486000/annotation2382021/range2382021/1

https://synbiohub.org/public/igem/BBa_K1486000/annotation2382021/range2382021/1
type: Range
persistent identity: range2382021
display id: range2382021
version: 1
direction: direction/forward
top level: BBa_K1486000
start: 1
end: 702
orientation: inline

BBa_I761011
type: ComponentDefinition
persistent identity: BBa I761011
display id: BBa_I761011
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CinR, CinL and glucose controlled promotor
creator: 2007 NYMU_Taiwan iGEM Team
created: 2007-10-18T11:00:00Z
modified: 2015-08-31T04:08:08Z
discontinued: false
dominant: false
experience: experience/None
group u list: _140_
m user id: 0
owner id: 2031
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This promotor is controlled by cinr, cinl and extrcellular glucose concentration. High extracellular glucose concentration will inhibit this promotor through the action of phosphorylated OmpR. Phosphorylated OmpR will bind the OmpR binding site of the promotor and block the movement of RNA polymerase. In contrast, the combined action of CinR and CinL will turn on the promotor. But only when the extracellular glucose level is low so OmpR dephosphorylate and dissociate from its binding site will this promotor become active. In essence, this promotor function as an AND gate, it will turn on if and only if the extracellular glucose level is low and there are some functional CinR and CinL protein inside the cell.
mutable notes: The key to the function of the promotor is the inhibitory ability of OmpR binding site. To ensure maximum affinity with phosphorylated OmpR, we use all three OmpR binding site.s.
mutable provenance: *BBa_R0078 *BBa_I761001
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
component: BBa_I761001
component: BBa_R0078
sequence annotation: BBa_R0078
sequence annotation: BBa_I761001
sequence: https://synbiohub.org/public/igem/BBa_I761011_sequence/1

BBa_I761001
type: Component
persistent identity: component1950122
display id: component1950122
version: 1
top level: BBa_I761011
access: public
definition: https://synbiohub.org/public/igem/BBa_I761001/1

BBa_R0078
type: Component
persistent identity: component1950118
display id: component1950118
version: 1
top level: BBa_I761011
access: public
definition: cinR

BBa_R0078
type: SequenceAnnotation
persistent identity: annotation1950118
display id: annotation1950118
version: 1
top level: BBa_I761011
location: https://synbiohub.org/public/igem/BBa_I761011/annotation1950118/range1950118/1
component: BBa_R0078

https://synbiohub.org/public/igem/BBa_I761011/annotation1950118/range1950118/1
type: Range
persistent identity: range1950118
display id: range1950118
version: 1
direction: direction/reverse
top level: BBa_I761011
start: 1
end: 225
orientation: inline

BBa_I761001
type: SequenceAnnotation
persistent identity: annotation1950122
display id: annotation1950122
version: 1
top level: BBa_I761011
location: https://synbiohub.org/public/igem/BBa_I761011/annotation1950122/range1950122/1
component: BBa_I761001

https://synbiohub.org/public/igem/BBa_I761011/annotation1950122/range1950122/1
type: Range
persistent identity: range1950122
display id: range1950122
version: 1
direction: direction/reverse
top level: BBa_I761011
start: 234
end: 295
orientation: inline

BBa_K091105
type: ComponentDefinition
persistent identity: BBa K091105
display id: BBa_K091105
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pTet/Mnt Hybrid Promoter
creator: Robert Cool
created: 2008-06-18T11:00:00Z
modified: 2015-05-08T01:08:37Z
discontinued: false
dominant: false
experience: experience/None
group u list: _191_
m user id: 0
owner id: 3080
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This promoter is a modified version of the Mnt promoter that is also responsive to TetR. The promoter should be repressed by Mnt repressor. It should also be repressed by TetR, and in the absence of Mnt repressor, should be induced by aTc.
mutable notes: Mnt repressor binds as a tetramer to two half-operator sites. Introduction of TetR binding sites between the Mnt promoter -10 sequence and the start site for transcription should allow for repression by TetR. Accordingly, the hybrid promoter was designed by 1) Remove all bases of mnt promoter 3??? to -10: gagtcgtattaattt will be replaced by tccctatcagtgata, and 2) truncate the second half of ptet -10 and mutate -35 so that it function to bind tetR (replace ttgaca with actgta), but is not a promoter, and 3) place the tetR1 and tetR2 binding sites 3??? to truncated mnt -10 sequence.
mutable provenance: This part comes from the Mnt promoter and the TetR promoter
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: Mnt
sequence annotation: tetR1
sequence annotation: -10
sequence annotation: tetR2
sequence: https://synbiohub.org/public/igem/BBa_K091105_sequence/1

Mnt
type: SequenceAnnotation
persistent identity: annotation1963742
display id: annotation1963742
version: 1
top level: BBa_K091105
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K091105/annotation1963742/range1963742/1

https://synbiohub.org/public/igem/BBa_K091105/annotation1963742/range1963742/1
type: Range
persistent identity: range1963742
display id: range1963742
version: 1
direction: direction/forward
top level: BBa_K091105
start: 1
end: 52
orientation: inline

tetR1
type: SequenceAnnotation
persistent identity: annotation1963740
display id: annotation1963740
version: 1
top level: BBa_K091105
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K091105/annotation1963740/range1963740/1

https://synbiohub.org/public/igem/BBa_K091105/annotation1963740/range1963740/1
type: Range
persistent identity: range1963740
display id: range1963740
version: 1
direction: direction/forward
top level: BBa_K091105
start: 53
end: 71
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1963739
display id: annotation1963739
version: 1
top level: BBa_K091105
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K091105/annotation1963739/range1963739/1

https://synbiohub.org/public/igem/BBa_K091105/annotation1963739/range1963739/1
type: Range
persistent identity: range1963739
display id: range1963739
version: 1
direction: direction/forward
top level: BBa_K091105
start: 47
end: 52
orientation: inline

tetR2
type: SequenceAnnotation
persistent identity: annotation1963741
display id: annotation1963741
version: 1
top level: BBa_K091105
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K091105/annotation1963741/range1963741/1

https://synbiohub.org/public/igem/BBa_K091105/annotation1963741/range1963741/1
type: Range
persistent identity: range1963741
display id: range1963741
version: 1
direction: direction/forward
top level: BBa_K091105
start: 78
end: 98
orientation: inline

BBa_R2114
type: ComponentDefinition
persistent identity: BBa R2114
display id: BBa_R2114
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter with operator site for C2003
creator: Reshma Shetty
created: 2006-10-22T11:00:00Z
modified: 2015-05-08T01:14:16Z
discontinued: false
dominant: false
group u list: _41_
m user id: 0
owner id: 126
owning group id: 70
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This promoter is designed to bind to C2003.
mutable notes: The placement of the operator site was designed to be near optimal for steric interference between C2003 and RNA polymerase.
mutable provenance: This part was inadvertently made while trying to make BBa_R2111. It contains a single base pair mutation in the -10 site of BBa_R2111.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: C2003 operator site
sequence annotation: transcription start site
sequence annotation: -10 site
sequence annotation: -35 site
sequence: https://synbiohub.org/public/igem/BBa_R2114_sequence/1

C2003 operator site
type: SequenceAnnotation
persistent identity: annotation1904662
display id: annotation1904662
version: 1
top level: BBa_R2114
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_R2114/annotation1904662/range1904662/1

https://synbiohub.org/public/igem/BBa_R2114/annotation1904662/range1904662/1
type: Range
persistent identity: range1904662
display id: range1904662
version: 1
direction: direction/forward
top level: BBa_R2114
start: 23
end: 34
orientation: inline

transcription start site
type: SequenceAnnotation
persistent identity: annotation1904661
display id: annotation1904661
version: 1
top level: BBa_R2114
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_R2114/annotation1904661/range1904661/1

https://synbiohub.org/public/igem/BBa_R2114/annotation1904661/range1904661/1
type: Range
persistent identity: range1904661
display id: range1904661
version: 1
direction: direction/forward
top level: BBa_R2114
start: 53
end: 53
orientation: inline

-10 site
type: SequenceAnnotation
persistent identity: annotation1904659
display id: annotation1904659
version: 1
top level: BBa_R2114
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R2114/annotation1904659/range1904659/1

https://synbiohub.org/public/igem/BBa_R2114/annotation1904659/range1904659/1
type: Range
persistent identity: range1904659
display id: range1904659
version: 1
direction: direction/forward
top level: BBa_R2114
start: 40
end: 45
orientation: inline

-35 site
type: SequenceAnnotation
persistent identity: annotation1904660
display id: annotation1904660
version: 1
top level: BBa_R2114
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R2114/annotation1904660/range1904660/1

https://synbiohub.org/public/igem/BBa_R2114/annotation1904660/range1904660/1
type: Range
persistent identity: range1904660
display id: range1904660
version: 1
direction: direction/forward
top level: BBa_R2114
start: 17
end: 22
orientation: inline

tADH1
type: ComponentDefinition
persistent identity: BBa J63002
display id: BBa_J63002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: ADH1 terminator from S. cerevisiae
creator: Caroline Ajo-Franklin
created: 2006-10-10T11:00:00Z
modified: 2015-08-31T01:56:26Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _97_
m user id: 0
owner id: 545
owning group id: 97
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: ADH1 terminator from S. cerevisiae
mutable notes: starts with stop codon
mutable provenance: genomic DNA of S. cerevisiae
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: ADH1 terminator
sequence: https://synbiohub.org/public/igem/BBa_J63002_sequence/1

ADH1 terminator
type: SequenceAnnotation
persistent identity: annotation1902851
display id: annotation1902851
version: 1
top level: tADH1
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_J63002/annotation1902851/range1902851/1

https://synbiohub.org/public/igem/BBa_J63002/annotation1902851/range1902851/1
type: Range
persistent identity: range1902851
display id: range1902851
version: 1
direction: direction/forward
top level: tADH1
start: 1
end: 225
orientation: inline

BBa_K259010
type: ComponentDefinition
persistent identity: BBa K259010
display id: BBa_K259010
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: AraC-RBS-FhuA
creator: Petros Mina
created: 2009-10-12T11:00:00Z
modified: 2015-05-08T01:11:42Z
discontinued: false
dominant: false
group u list: _357_
m user id: 0
owner id: 5318
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: ==What this part gives upon expression== This part encodes for FhuA directly as it is already in frame with a pBAD/Arabinose regulated promoter. Promoter Induction can occur with 0.1% Arabinose in the culture environment. FhuA is an E.coli Outer Membrane (OM) protein used for scavenging iron from the nutrient medium. For more information on FhuA visit the FhuA main page ( K259000 ). ==Potential uses of this Device== This part can be used also to create protein fusions by adding other proteins to it's 3' end. Protein fusion can be accomplished straightforwardly using the Bioscaffold-Linker biobricks K259003 . Protein fusions with FhuA may allow delivery of proteins to the OM of E.coli cells or inclusion into Outer Membrane Vesicles (see iGEM'09 Bristol-BCCS project).
mutable notes: The part was assembled using standard RFC10 assembly.
mutable provenance: The arabinose promoter occurs naturally in E.coli. The RBS is designed from the Anderson family. FhuA also occurs naturally in E.coli.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_R0081
component: BBa_J61100
component: BBa_R0080
component: BBa_K259000
sequence annotation: BBa_J61100
sequence annotation: BBa_R0080
sequence annotation: BBa_K259000
sequence annotation: BBa_R0081
sequence: https://synbiohub.org/public/igem/BBa_K259010_sequence/1

BBa_R0081
type: Component
persistent identity: component2037536
display id: component2037536
version: 1
top level: BBa_K259010
access: public
definition: https://synbiohub.org/public/igem/BBa_R0081/1

BBa_J61100
type: Component
persistent identity: component2037544
display id: component2037544
version: 1
top level: BBa_K259010
access: public
definition: BBa_J61100

BBa_R0080
type: Component
persistent identity: component2037543
display id: component2037543
version: 1
top level: BBa_K259010
access: public
definition: https://synbiohub.org/public/igem/BBa_R0080/1

BBa_K259000
type: Component
persistent identity: component2037549
display id: component2037549
version: 1
top level: BBa_K259010
access: public
definition: fhuA

BBa_J61100
type: SequenceAnnotation
persistent identity: annotation2037544
display id: annotation2037544
version: 1
top level: BBa_K259010
location: https://synbiohub.org/public/igem/BBa_K259010/annotation2037544/range2037544/1
component: BBa_J61100

https://synbiohub.org/public/igem/BBa_K259010/annotation2037544/range2037544/1
type: Range
persistent identity: range2037544
display id: range2037544
version: 1
direction: direction/reverse
top level: BBa_K259010
start: 349
end: 360
orientation: inline

BBa_R0080
type: SequenceAnnotation
persistent identity: annotation2037543
display id: annotation2037543
version: 1
top level: BBa_K259010
location: https://synbiohub.org/public/igem/BBa_K259010/annotation2037543/range2037543/1
component: BBa_R0080

https://synbiohub.org/public/igem/BBa_K259010/annotation2037543/range2037543/1
type: Range
persistent identity: range2037543
display id: range2037543
version: 1
direction: direction/reverse
top level: BBa_K259010
start: 192
end: 340
orientation: inline

BBa_K259000
type: SequenceAnnotation
persistent identity: annotation2037549
display id: annotation2037549
version: 1
top level: BBa_K259010
location: https://synbiohub.org/public/igem/BBa_K259010/annotation2037549/range2037549/1
component: BBa_K259000

https://synbiohub.org/public/igem/BBa_K259010/annotation2037549/range2037549/1
type: Range
persistent identity: range2037549
display id: range2037549
version: 1
direction: direction/reverse
top level: BBa_K259010
start: 367
end: 2613
orientation: inline

BBa_R0081
type: SequenceAnnotation
persistent identity: annotation2037536
display id: annotation2037536
version: 1
top level: BBa_K259010
location: https://synbiohub.org/public/igem/BBa_K259010/annotation2037536/range2037536/1
component: BBa_R0081

https://synbiohub.org/public/igem/BBa_K259010/annotation2037536/range2037536/1
type: Range
persistent identity: range2037536
display id: range2037536
version: 1
direction: direction/reverse
top level: BBa_K259010
start: 1
end: 183
orientation: inline

Pnot(O16)
type: ComponentDefinition
persistent identity: BBa I732271
display id: BBa_I732271
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Family Member with Hybrid Operator (D001O16)
creator: Ma Rui
created: 2007-10-13T11:00:00Z
modified: 2015-08-31T04:07:57Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732271_sequence/1

BBa_K137030
type: ComponentDefinition
persistent identity: BBa K137030
display id: BBa_K137030
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: constitutive promoter with (TA)9 between -10 and -35 elements
creator: Allen Lin
created: 2008-07-20T11:00:00Z
modified: 2015-05-08T01:10:09Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _187_
m user id: 0
owner id: 3112
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: constitutive promoter with (TA)9 between -10 and -35 elements
mutable notes: With 9 TA repeats between the -10 and -35 elements, the distance between the two elements is not optimal for the sigma factor to bind to the promoter. Thus, the promoter should be in the 'off' state.
mutable provenance: This part was PCR amplified from two synthesized primers that bound to each other.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: https://synbiohub.org/public/igem/BBa_K137030/annotation1967767/1
sequence annotation: https://synbiohub.org/public/igem/BBa_K137030/annotation1967766/1
sequence: https://synbiohub.org/public/igem/BBa_K137030_sequence/1