//dna/spacer
type: Collection
persistent identity: dna spacer
display id: dna_spacer
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
member: spacer
member: BioScaffol
member: BBa_K259008
member: BBa_K259009

//dna/conjugation
type: Collection
persistent identity: dna conjugation
display id: dna_conjugation
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
member: OriT-RP4
member: OriTR
member: oriT/LacZ
member: BBa_K175000
member: BBa_I714031
member: BBa_I714030
member: BBa_K175001
member: OriTF
member: BBa_I714032
member: oriTr/LacZ

//dna/nucleotide
type: Collection
persistent identity: dna nucleotide
display id: dna_nucleotide
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
member: C
member: T
member: G
member: A
member: BBa_K649204
member: BBa_M34014
member: Message

//dna/origami
type: Collection
persistent identity: dna origami
display id: dna_origami
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
member: 4-fingers
member: BBa_J35000
member: 7-fingers
member: B-Z DNA sw
member: dumbbell
member: BBa_K1479005
member: BBa_J35001
member: 3-fingers
member: 5-fingers
member: 6-fingers

//dna/bioscaffold
type: Collection
persistent identity: dna bioscaffold
display id: dna_bioscaffold
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
member: BioScaffol
member: BBa_J70030
member: BBa_J70034
member: BBa_J70032
member: BBa_K259009
member: BBa_J70012
member: BBa_J70052
member: BBa_J70050
member: tetO array
member: BBa_J70036
member: BBa_K259008
member: BBa_J70040
member: BBa_J70010

//dna
type: Collection
persistent identity: dna
display id: dna
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
member: O3
member: Orai1 V102
member: O4
member: IscA1
member: //dna/nucleotide
member: //dna/chromosomalintegration
member: BBa_K1614009
member: //dna/cloningsite
member: BBa_K1680005
member: //dna/spacer
member: Tetrahedro
member: //dna/restrictionsite
member: O5
member: BBa_K1614013
member: BBa_K1680014
member: IscA2
member: //dna/transposon
member: BBa_K1680015
member: MDA
member: //dna/origami
member: O1
member: //dna/conjugation
member: //dna/transposome
member: BBa_K1614007
member: Active Com
member: BBa_K1680016
member: O2
member: //dna/origin_of_replication
member: //dna/aptamer
member: //dna/primerbindingsite
member: //dna/bioscaffold
member: //dna/scar

//dna/cloningsite
type: Collection
persistent identity: dna cloningsite
display id: dna_cloningsite
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
member: Adapter
member: BBa_I13452
member: BBa_J56011
member: BBa_I13456
member: BBa_I13455
member: BBa_I13457
member: Suffix
member: Prefix
member: BBa_I13450

//dna/primerbindingsite
type: Collection
persistent identity: dna primerbindingsite
display id: dna_primerbindingsite
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
member: VR
member: Suffix
member: VF2
member: Prefix

//dna/transposome/tn5
type: Collection
persistent identity: dna transposome tn5
display id: dna_transposome_tn5
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
member: BBa_M1001
member: BBa_M1000

//dna/scar
type: Collection
persistent identity: dna scar
display id: dna_scar
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
member: A scar
member: SX scar
member: Scar 25
member: RFC 37
member: AvrII/XbaI
member: scar
member: Scar25 TAA
member: BBa_K106016
member: Scar25 ATG
member: BBa_K106017

//dna/transposon
type: Collection
persistent identity: dna transposon
display id: dna_transposon
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
member: BBa_K510000
member: Tn5F
member: Tn5R
member: BBa_K1973006

//dna/transposome
type: Collection
persistent identity: dna transposome
display id: dna_transposome
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
member: //dna/transposome/tn5

//dna/origin_of_replication
type: Collection
persistent identity: dna origin of replication
display id: dna_origin_of_replication
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
member: mt ori7

//dna/restrictionsite
type: Collection
persistent identity: dna restrictionsite
display id: dna_restrictionsite
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
member: BBa_I13451
member: AatII
member: NotI
member: SapI
member: NsiI
member: BbvCI
member: BclI
member: BsiWI
member: XbaI
member: I-SceI RS
member: PvuI
member: PspOMI
member: BfuAI
member: Acc651
member: SpeI
member: SacII
member: AclI
member: ApaI
member: BglII
member: SapI (reve
member: EagI
member: MluI
member: BioScaffol
member: KpnI
member: AfeI
member: AscI
member: BamHI
member: BsrG1
member: BBa_K259009
member: Acc65I
member: XmaI
member: EcoRI
member: BccI
member: NheI
member: BfaI
member: BBa_K175002
member: AseI
member: BssHII
member: BamHI rs
member: SalI
member: HpaI
member: AvrII
member: HindIII
member: MfeI
member: BBa_K259008
member: BciVI
member: BmtI

//dna/chromosomalintegration
type: Collection
persistent identity: dna chromosomalintegration
display id: dna_chromosomalintegration
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
member: Down
member: amyE 3 IS
member: K592200
member: BBa_K510000
member: BBa_K1930003
member: BBa_K1973006
member: K592202
member: Up
member: amyE 5 IS
member: BBa_K1930002

//dna/aptamer
type: Collection
persistent identity: dna aptamer
display id: dna_aptamer
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
member: BBa_K1330000
member: BBa_K1636000
member: BBa_J36854
member: BBa_J36856
member: BBa_K598001
member: BBa_J36859
member: BBa_K2027049
member: BBa_K1614009
member: BBa_J36857
member: BBa_J36855
member: BBa_J36858
member: BBa_J36853
member: BBa_K1614013
member: BBa_K2027050

BBa_I13450
type: ComponentDefinition
persistent identity: BBa I13450
display id: BBa_I13450
version: 1
was generated by: iGEM to SBOL conversion
description: Removes BioBrick End
created: 2005-05-24T11:00:00Z
modified: 2015-08-31T04:07:34Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Contains both an early MfeI site and a late NsiI site. Cutting with Mfe and Spe and ligation to a BB part cut EX will form two mixed sites, neither of which can be recut. Cutting with Xba and Nsi and ligation to a BB part cut SP will again form two mixed sites, neither of which can be recut.
discontinued: false
dominant: false
experience: experience/Works
group u list: _11_
m user id: 0
owner id: 253
owning group id: 6
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Should be grown in pSB1A2, as Nsi cuts both pSB2K3 and pSB1AK3 and Mfe cuts pSB4A3. 70 bp from Xba to Nsi or Mfe to Spe, the minimum for gel extraction. The middle is largely noise, to raise the size of the part.
star: false
creator: jkm
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: MfeI
sequence annotation: NsiI
sequence: https://synbiohub.org/public/igem/BBa_I13450_sequence/1

MfeI
type: SequenceAnnotation
persistent identity: annotation1494720
display id: annotation1494720
version: 1
top level: BBa_I13450
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I13450/annotation1494720/range1494720/1

https://synbiohub.org/public/igem/BBa_I13450/annotation1494720/range1494720/1
type: Range
persistent identity: range1494720
display id: range1494720
version: 1
top level: BBa_I13450
direction: direction/forward
start: 2
end: 7
orientation: inline

NsiI
type: SequenceAnnotation
persistent identity: annotation1494630
display id: annotation1494630
version: 1
top level: BBa_I13450
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I13450/annotation1494630/range1494630/1

https://synbiohub.org/public/igem/BBa_I13450/annotation1494630/range1494630/1
type: Range
persistent identity: range1494630
display id: range1494630
version: 1
top level: BBa_I13450
direction: direction/forward
start: 68
end: 73
orientation: inline

BBa_K1973000
type: ComponentDefinition
persistent identity: BBa K1973000
display id: BBa_K1973000
version: 1
was generated by: iGEM to SBOL conversion
description: lapG
created: 2016-10-08T11:00:00Z
modified: 2016-10-12T05:16:10Z
mutable provenance: Obtained by PCR from the genome of Pseudomonas putida KT2442.
owned by: james
owned by: myers
mutable description: Codifies the LapG protein from Pseudomonas putida KT2442, a periplasmic protease that participates in the biofilm dispersion process by cleaving adhesion proteins such as LapA.
discontinued: false
dominant: false
experience: experience/None
group u list: _2440_
m user id: 30017
owner id: 30003
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: We have inlcuded the sequence corresponding to RBS BBa_B0034 with the PCR primers used for its amplification, as well as the scar it should have been produced by the clonning of both parts
star: false
creator: Laura Claret Fern??ndez
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: BBa_B0034
sequence annotation: lapG
sequence annotation: scar
sequence: https://synbiohub.org/public/igem/BBa_K1973000_sequence/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2489352
display id: annotation2489352
version: 1
top level: BBa_K1973000
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1973000/annotation2489352/range2489352/1

https://synbiohub.org/public/igem/BBa_K1973000/annotation2489352/range2489352/1
type: Range
persistent identity: range2489352
display id: range2489352
version: 1
top level: BBa_K1973000
direction: direction/forward
start: 1
end: 12
orientation: inline

lapG
type: SequenceAnnotation
persistent identity: annotation2489353
display id: annotation2489353
version: 1
top level: BBa_K1973000
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1973000/annotation2489353/range2489353/1

https://synbiohub.org/public/igem/BBa_K1973000/annotation2489353/range2489353/1
type: Range
persistent identity: range2489353
display id: range2489353
version: 1
top level: BBa_K1973000
direction: direction/forward
start: 19
end: 663
orientation: inline

scar
type: SequenceAnnotation
persistent identity: annotation2489354
display id: annotation2489354
version: 1
top level: BBa_K1973000
role: feature/scar
location: https://synbiohub.org/public/igem/BBa_K1973000/annotation2489354/range2489354/1

https://synbiohub.org/public/igem/BBa_K1973000/annotation2489354/range2489354/1
type: Range
persistent identity: range2489354
display id: range2489354
version: 1
top level: BBa_K1973000
direction: direction/forward
start: 13
end: 18
orientation: inline

OriTR
type: ComponentDefinition
persistent identity: BBa J01003
display id: BBa_J01003
version: 1
was generated by: iGEM to SBOL conversion
description: OriT-R (Origin of transfer for the R-plasmid nic region)
created: 2005-10-17T11:00:00Z
modified: 2015-08-31T04:08:11Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: OriTR, the R plasmid nic region, is where the relaxosome nicks the plasmid and conjugative transfer by R plasmid machinery begins.
discontinued: false
dominant: false
experience: experience/None
group u list: _13_
m user id: 0
owner id: 395
owning group id: 13
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Golden Bear
type: DnaRegion
role: partType/Conjugation
role: SO:0000724
sequence: https://synbiohub.org/public/igem/BBa_J01003_sequence/1

BBa_I13456
type: ComponentDefinition
persistent identity: BBa I13456
display id: BBa_I13456
version: 1
was generated by: iGEM to SBOL conversion
description: Insertion site plus RNAse E
created: 2005-07-18T11:00:00Z
modified: 2015-08-31T04:07:34Z
owned by: james
owned by: myers
mutable description: Shortened RNAse E site (Nilsson et al. 1996) plus shortened insertion site (I13452)
discontinued: false
dominant: false
experience: experience/None
group u list: _11_
m user id: 0
owner id: 253
owning group id: 6
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: jkm
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: RNAse E cut site (between the bases)
sequence annotation: Insertion site
sequence annotation: RNAse E site
sequence: https://synbiohub.org/public/igem/BBa_I13456_sequence/1

RNAse E cut site (between the bases)
type: SequenceAnnotation
persistent identity: annotation1588505
display id: annotation1588505
version: 1
top level: BBa_I13456
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I13456/annotation1588505/range1588505/1

https://synbiohub.org/public/igem/BBa_I13456/annotation1588505/range1588505/1
type: Range
persistent identity: range1588505
display id: range1588505
version: 1
top level: BBa_I13456
direction: direction/forward
start: 9
end: 10
orientation: inline

Insertion site
type: SequenceAnnotation
persistent identity: annotation1588506
display id: annotation1588506
version: 1
top level: BBa_I13456
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I13456/annotation1588506/range1588506/1

https://synbiohub.org/public/igem/BBa_I13456/annotation1588506/range1588506/1
type: Range
persistent identity: range1588506
display id: range1588506
version: 1
top level: BBa_I13456
direction: direction/forward
start: 22
end: 55
orientation: inline

RNAse E site
type: SequenceAnnotation
persistent identity: annotation1588495
display id: annotation1588495
version: 1
top level: BBa_I13456
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I13456/annotation1588495/range1588495/1

https://synbiohub.org/public/igem/BBa_I13456/annotation1588495/range1588495/1
type: Range
persistent identity: range1588495
display id: range1588495
version: 1
top level: BBa_I13456
direction: direction/forward
start: 1
end: 21
orientation: inline

BBa_K2027050
type: ComponentDefinition
persistent identity: BBa K2027050
display id: BBa_K2027050
version: 1
was generated by: iGEM to SBOL conversion
description: PQQ Aptamer #2 (15ADb11)
created: 2016-10-23T11:00:00Z
modified: 2016-10-27T10:46:11Z
mutable provenance: Emahi et al. 2014 (http://www.rsc.org/suppdata/ra/c4/c4ra11052h/c4ra11052h1.pdf)
owned by: james
owned by: myers
mutable description: This is a functional DNA sequence that encodes the pyrilloquinone quinone binding aptamer. It can be used in both binding and sensing applications.
discontinued: false
dominant: false
experience: experience/None
group u list: _2494_
m user id: 29123
owner id: 29123
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: This was one of the best two DNA aptamer candidates from SELEX (also see BBa_K2027050).
star: false
creator: Michael Becich
type: DnaRegion
role: partType/Other
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_K2027050_sequence/1

BBa_I13455
type: ComponentDefinition
persistent identity: BBa I13455
display id: BBa_I13455
version: 1
was generated by: iGEM to SBOL conversion
description: Insertion site plus RNAse E cut site
created: 2005-07-17T11:00:00Z
modified: 2015-08-31T04:07:34Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RNAse E site (Smolke et al., 2002) followed by insertion site (shortened I13452)
discontinued: false
dominant: false
experience: experience/None
group u list: _11_
m user id: 0
owner id: 253
owning group id: 6
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: jkm
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: Insertion site
sequence annotation: RNAse E cut site (between the bases)
sequence annotation: RNAse E site
sequence: https://synbiohub.org/public/igem/BBa_I13455_sequence/1

Insertion site
type: SequenceAnnotation
persistent identity: annotation1588486
display id: annotation1588486
version: 1
top level: BBa_I13455
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I13455/annotation1588486/range1588486/1

https://synbiohub.org/public/igem/BBa_I13455/annotation1588486/range1588486/1
type: Range
persistent identity: range1588486
display id: range1588486
version: 1
top level: BBa_I13455
direction: direction/forward
start: 45
end: 78
orientation: inline

RNAse E cut site (between the bases)
type: SequenceAnnotation
persistent identity: annotation1588485
display id: annotation1588485
version: 1
top level: BBa_I13455
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I13455/annotation1588485/range1588485/1

https://synbiohub.org/public/igem/BBa_I13455/annotation1588485/range1588485/1
type: Range
persistent identity: range1588485
display id: range1588485
version: 1
top level: BBa_I13455
direction: direction/forward
start: 29
end: 30
orientation: inline

RNAse E site
type: SequenceAnnotation
persistent identity: annotation1588484
display id: annotation1588484
version: 1
top level: BBa_I13455
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I13455/annotation1588484/range1588484/1

https://synbiohub.org/public/igem/BBa_I13455/annotation1588484/range1588484/1
type: Range
persistent identity: range1588484
display id: range1588484
version: 1
top level: BBa_I13455
direction: direction/forward
start: 1
end: 44
orientation: inline

KpnI
type: ComponentDefinition
persistent identity: BBa I11071
display id: BBa_I11071
version: 1
was generated by: iGEM to SBOL conversion
description: KpnI restriction enzyme site with extra flanking nucleotides
created: 2004-09-06T11:00:00Z
modified: 2015-08-31T04:07:30Z
mutable provenance: NEB catalogue
owned by: james
owned by: myers
mutable description: KpnI restriction site with 3bp flanking each side
discontinued: false
dominant: false
experience: experience/None
group u list: _2_
m user id: 0
owner id: 102
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: bu
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: KpnI
sequence: https://synbiohub.org/public/igem/BBa_I11071_sequence/1

KpnI
type: SequenceAnnotation
persistent identity: annotation1147967
display id: annotation1147967
version: 1
top level: KpnI
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I11071/annotation1147967/range1147967/1

https://synbiohub.org/public/igem/BBa_I11071/annotation1147967/range1147967/1
type: Range
persistent identity: range1147967
display id: range1147967
version: 1
top level: KpnI
direction: direction/forward
start: 4
end: 9
orientation: inline

G
type: ComponentDefinition
persistent identity: BBa G0011
display id: BBa_G0011
version: 1
was generated by: iGEM to SBOL conversion
description: The nucleotide G
created: 2007-07-22T11:00:00Z
modified: 2015-08-31T04:07:28Z
mutable provenance: None.
owned by: james
owned by: myers
mutable description: This is a part consisting of only a single nucleotide ... G. It can act as a spacer between restriction sites and parts.
discontinued: false
dominant: false
experience: experience/None
group u list: _41_
m user id: 0
owner id: 126
owning group id: 162
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: None.
star: false
creator: Reshma Shetty
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_G0011_sequence/1

ApaI
type: ComponentDefinition
persistent identity: BBa I11080
display id: BBa_I11080
version: 1
was generated by: iGEM to SBOL conversion
description: ApaI restriction site with extra flanking nucleotides
created: 2004-09-06T11:00:00Z
modified: 2015-08-31T04:07:30Z
mutable provenance: NEB catalog
owned by: james
owned by: myers
mutable description: ApaI restriction site
discontinued: false
dominant: false
experience: experience/None
group u list: _2_
m user id: 0
owner id: 102
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: bu
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_I11080_sequence/1

O3
type: ComponentDefinition
persistent identity: BBa K2054003
display id: BBa_K2054003
version: 1
was generated by: iGEM to SBOL conversion
description: Oligo 3 of DNA Tetrahedral Nanostructure
creator: LAI HEI WAI
created: 2016-10-13T11:00:00Z
modified: 2016-10-27T11:56:35Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2522_
m user id: 0
m user id: 30316
owner id: 30316
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This will be used to build our desired nanostructure that target micro RNA biomarkers. This plasmid encodes the second ssDNA that will make the tetrahedral nanostructure, together with oligo 1, 2, 4 and 5.
mutable notes: The assembly of the structure.
mutable provenance: Elbaz, J., Yin, P., & Voigt, C. A. (2016). Genetic encoding of DNA nanostructures and their self-assembly in living bacteria. Nature communications, 7.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_K2054003_sequence/1

BBa_J35001
type: ComponentDefinition
persistent identity: BBa J35001
display id: BBa_J35001
version: 1
was generated by: iGEM to SBOL conversion
description: fork hairpin
created: 2006-10-17T11:00:00Z
modified: 2015-08-31T04:08:47Z
mutable provenance: Artificial sequence.
owned by: james
owned by: myers
mutable description: We use this sequence to insert it into staples as a level 2 pixel.
discontinued: false
dominant: false
experience: experience/None
group u list: _64_
m user id: 0
owner id: 807
owning group id: 64
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable notes: OligoAnalyzer 3.0 http://www.idtdna.com/analyzer/Applications/OligoAnalyzer/
star: false
creator: Andrey Kuznetsov
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_J35001_sequence/1

ApaI
type: ComponentDefinition
persistent identity: BBa M31955
display id: BBa_M31955
version: 1
was generated by: iGEM to SBOL conversion
description: ApaI restriction enzyme site
created: 2007-02-27T12:00:00Z
modified: 2015-05-08T01:14:01Z
mutable provenance: Known cut site
owned by: james
owned by: myers
mutable description: Restriction site
discontinued: false
dominant: true
experience: experience/None
group u list: _102_
m user id: 0
owner id: 1381
owning group id: 102
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: none
star: false
creator: Rebecca Adams
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_M31955_sequence/1

BBa_K259008
type: ComponentDefinition
persistent identity: BBa K259008
display id: BBa_K259008
version: 1
was generated by: iGEM to SBOL conversion
description: BioScaffold Linker - Removes Stop Codons & scars & replaces with a Gly-Ser linker
created: 2009-10-12T11:00:00Z
modified: 2015-05-08T01:11:42Z
mutable provenance: This part is completely designed de novo. This sequence does not occur naturally.
owned by: james
owned by: myers
mutable description: This part is part of a family of Bioscaffold - Linkers. This part will help users interested in protein-fusion. The usage of the part will allow in frame assembly of 2(+) proteins by: *Removing the stop codons from the upstream protein. *Removing the scar created by the Assembly Standard 10 (RFC10) construction of 2(+) protein coding sequences. *Maintaining the start codon of the downstream protein. *Adding a flexible (Gly-Ser-Gly) linker between the two protein coding sequences. *Acting as a translational buffer if ligation is incorrect. ===Assembly Instructions=== *Create an Assembly Standard 10 fusion as below ; **Upstream Protein Part - Bioscaffold Linker - Downstream Protein Part** *Use the BioScaffold Specific Enzymes (i)Restrict with BpuEI to remove upstream scar (created between Upstream Protein - Bioscaffold) and stop codons from upstream protein part. (ii)Ligate and you will get the intermediate product; **Upstream Protein (w/out Stop codons) - Tyr - BioScaffold Linker - Downstream Protein** *Restrict with CspCI to remove downstream scar. *Ligate to get the final product; **Upstream Protein - Tyr-Gly-Ser-Gly- Downstream Protein** ====N.B.==== CspCI cuts with a 1 b.p discrepancy depending on the sequence. However this should not affect the restriction/ligation process as this has been taken into account and incorporated into the design. Even with the discrepancy involved the restriction/ligation process will still give the same product and in-frame fusion. The other restriction variants of CspCI have also been taken into account and relevant BioScaffolds are being produced to be tested. Updates will soon follow on this. This version accounts for cleaving DNA 12/10bp (top/bottom) on the 5' of the recognition sequence and 10…
discontinued: false
dominant: false
group u list: _357_
m user id: 0
owner id: 5318
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: CspCI enzyme has different variations in the distance of restricting DNA from the recognition site.This biobrick is almost identical to part BBa_K259004 but modified to account for this discrepancy.
star: false
creator: Petros Mina
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: Gly-Ser-Gly Linker
sequence annotation: 5' CspCI recognition site
sequence annotation: Translational buffer
sequence annotation: Tyr involved in Restriction/Ligation
sequence annotation: Met involved in Restriction/Ligation
sequence annotation: 3' CspCI Recognition site
sequence annotation: BpuEI (rev.comp)
sequence annotation: BpuEI
sequence annotation: Translational Buffer
sequence: https://synbiohub.org/public/igem/BBa_K259008_sequence/1

Gly-Ser-Gly Linker
type: SequenceAnnotation
persistent identity: annotation2037353
display id: annotation2037353
version: 1
top level: BBa_K259008
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K259008/annotation2037353/range2037353/1

https://synbiohub.org/public/igem/BBa_K259008/annotation2037353/range2037353/1
type: Range
persistent identity: range2037353
display id: range2037353
version: 1
top level: BBa_K259008
direction: direction/forward
start: 44
end: 52
orientation: inline

5' CspCI recognition site
type: SequenceAnnotation
persistent identity: annotation2037356
display id: annotation2037356
version: 1
top level: BBa_K259008
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K259008/annotation2037356/range2037356/1

https://synbiohub.org/public/igem/BBa_K259008/annotation2037356/range2037356/1
type: Range
persistent identity: range2037356
display id: range2037356
version: 1
top level: BBa_K259008
direction: direction/forward
start: 65
end: 67
orientation: inline

Translational buffer
type: SequenceAnnotation
persistent identity: annotation2037358
display id: annotation2037358
version: 1
top level: BBa_K259008
role: feature/stop
role: SO:0000319
location: https://synbiohub.org/public/igem/BBa_K259008/annotation2037358/range2037358/1

https://synbiohub.org/public/igem/BBa_K259008/annotation2037358/range2037358/1
type: Range
persistent identity: range2037358
display id: range2037358
version: 1
top level: BBa_K259008
direction: direction/forward
start: 68
end: 72
orientation: inline

Tyr involved in Restriction/Ligation
type: SequenceAnnotation
persistent identity: annotation2037351
display id: annotation2037351
version: 1
top level: BBa_K259008
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K259008/annotation2037351/range2037351/1

https://synbiohub.org/public/igem/BBa_K259008/annotation2037351/range2037351/1
type: Range
persistent identity: range2037351
display id: range2037351
version: 1
top level: BBa_K259008
direction: direction/forward
start: 41
end: 43
orientation: inline

Met involved in Restriction/Ligation
type: SequenceAnnotation
persistent identity: annotation2037354
display id: annotation2037354
version: 1
top level: BBa_K259008
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K259008/annotation2037354/range2037354/1

https://synbiohub.org/public/igem/BBa_K259008/annotation2037354/range2037354/1
type: Range
persistent identity: range2037354
display id: range2037354
version: 1
top level: BBa_K259008
direction: direction/forward
start: 53
end: 55
orientation: inline

3' CspCI Recognition site
type: SequenceAnnotation
persistent identity: annotation2037357
display id: annotation2037357
version: 1
top level: BBa_K259008
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K259008/annotation2037357/range2037357/1

https://synbiohub.org/public/igem/BBa_K259008/annotation2037357/range2037357/1
type: Range
persistent identity: range2037357
display id: range2037357
version: 1
top level: BBa_K259008
direction: direction/forward
start: 73
end: 76
orientation: inline

BpuEI (rev.comp)
type: SequenceAnnotation
persistent identity: annotation2037350
display id: annotation2037350
version: 1
top level: BBa_K259008
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K259008/annotation2037350/range2037350/1

https://synbiohub.org/public/igem/BBa_K259008/annotation2037350/range2037350/1
type: Range
persistent identity: range2037350
display id: range2037350
version: 1
top level: BBa_K259008
direction: direction/reverse
start: 3
end: 8
orientation: inline

BpuEI
type: SequenceAnnotation
persistent identity: annotation2037352
display id: annotation2037352
version: 1
top level: BBa_K259008
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K259008/annotation2037352/range2037352/1

https://synbiohub.org/public/igem/BBa_K259008/annotation2037352/range2037352/1
type: Range
persistent identity: range2037352
display id: range2037352
version: 1
top level: BBa_K259008
direction: direction/forward
start: 21
end: 26
orientation: inline

Translational Buffer
type: SequenceAnnotation
persistent identity: annotation2037355
display id: annotation2037355
version: 1
top level: BBa_K259008
role: feature/stop
role: SO:0000319
location: https://synbiohub.org/public/igem/BBa_K259008/annotation2037355/range2037355/1

https://synbiohub.org/public/igem/BBa_K259008/annotation2037355/range2037355/1
type: Range
persistent identity: range2037355
display id: range2037355
version: 1
top level: BBa_K259008
direction: direction/forward
start: 56
end: 64
orientation: inline

Orai1 V102
type: ComponentDefinition
persistent identity: BBa K2028000
display id: BBa_K2028000
version: 1
was generated by: iGEM to SBOL conversion
description: This part is the cDNA of a mutated calcium ion channel protein.
creator: Zhongxiu Hu
created: 2016-10-13T11:00:00Z
modified: 2016-10-29T08:21:59Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2495_
m user id: 30292
owner id: 30292
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This part encodes the gene of Calcium release-activated calcium channel protein 1 (Orai1), which is a part of calcium selective ion channel in humans. Orai1 calcium channels in the plasma membrane are activated by stromal interaction molecule-1 (STIM1), an endoplasmic reticulum calcium sensor, to mediate store-operated calcium entry (SOCE).Store-operated calcium (Ca2+) entry (SOCE) is activated in response to cellular stimuli that deplete the sarco/endoplasmic reticulum (ER) lumen of Ca2+, a prerequisite event that opens exquisitely Ca2+ selective plasma membrane (PM) channels, augmenting cytosolic Ca2+ levels and refilling ER stores. The principal molecular components of SOCE in many cell types include the ER-inserted stromal interaction molecule-1 (STIM1)3,4 and the Orai1 PM channel subunits5,6,7,8, which physically interact at ER???PM junctions in a functional Ca2+ release-activated Ca2+ (CRAC) channel complex. SOCE through CRAC channels is integral to myriad signalling pathways in electrically excitable and non-excitable cells, the most prominent being immune cells in which inheritable mutations in STIM1 and Orai1 have been linked to devastating immunodeficiency diseases. Therefore, we use this part to help with eliminating Ca2+ in hard water.
mutable notes: Usually, the protein Orai1 works only when the STIM1 channel is activated. However, we select the mutated protein that did not require the presistence of STIM1, which changed the 102th amino acid valine to alanine.
mutable provenance: This part comes from the cDNA of mutated Orai1 protein (V102A).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
sequence: https://synbiohub.org/public/igem/BBa_K2028000_sequence/1

BBa_I714031
type: ComponentDefinition
persistent identity: BBa I714031
display id: BBa_I714031
version: 1
was generated by: iGEM to SBOL conversion
description: OriT-R (Origin of Transfer for the R751-plasmid nic region)
created: 2007-10-20T11:00:00Z
modified: 2015-08-31T04:07:47Z
mutable provenance: Enterobacter aerogenes plasmid R751 NCBI:NC_001735
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: OriT-R751 (Origin of Transfer for the R751-type conjugative plasmid) is the nic region, where the relaxosome nicks the plasmid and conjugative transfer by R751 plasmid machinery begins via rolling circle replication.
discontinued: false
dominant: false
experience: experience/Works
group u list: _142_
m user id: 0
owner id: 2248
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Mingzhi Qu
type: DnaRegion
role: partType/Conjugation
role: SO:0000724
sequence: https://synbiohub.org/public/igem/BBa_I714031_sequence/1

BBa_J36853
type: ComponentDefinition
persistent identity: BBa J36853
display id: BBa_J36853
version: 1
was generated by: iGEM to SBOL conversion
description: T50 linkaptamer
created: 2006-10-28T11:00:00Z
modified: 2015-08-31T04:08:47Z
mutable provenance: synthetic
owned by: james
owned by: myers
mutable description: T50 is the thrombin-binding component of the A50 adaptamer which can physically link together the proteins thrombin and streptavidin.
discontinued: false
dominant: false
experience: experience/None
group u list: _65_
m user id: 0
owner id: 1245
owning group id: 65
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Linking region had to avoid base-pairing with aptamer sequences.
star: false
creator: Lewis Hahn
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_J36853_sequence/1

BBa_J70036
type: ComponentDefinition
persistent identity: BBa J70036
display id: BBa_J70036
version: 1
was generated by: iGEM to SBOL conversion
description: A BioScaffold Part (Uses AarI) for Library Insert Preparation (see Part Design page)
created: 2008-12-27T12:00:00Z
modified: 2015-05-08T01:08:20Z
mutable provenance: Designed
owned by: james
owned by: myers
mutable description: When placed in a BioBrick assembly and cut with the restriction enzyme AarI, this part will direct the restriction enzyme to cut within the BioBrick scar still in testing, uses AarI (2) XmaI (1) See part design page or BBFRFC15, for details about use
discontinued: false
dominant: false
experience: experience/None
group u list: _41_
m user id: 0
owner id: 1201
owning group id: 41
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: TBA
star: false
creator: Julie Norville
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: XmaI
sequence annotation: AarI
sequence: https://synbiohub.org/public/igem/BBa_J70036_sequence/1

XmaI
type: SequenceAnnotation
persistent identity: annotation2000052
display id: annotation2000052
version: 1
top level: BBa_J70036
role: feature/dna
role: SO:0000110
location: https://synbiohub.org/public/igem/BBa_J70036/annotation2000052/range2000052/1

https://synbiohub.org/public/igem/BBa_J70036/annotation2000052/range2000052/1
type: Range
persistent identity: range2000052
display id: range2000052
version: 1
top level: BBa_J70036
direction: direction/forward
start: 23
end: 28
orientation: inline

AarI
type: SequenceAnnotation
persistent identity: annotation2000051
display id: annotation2000051
version: 1
top level: BBa_J70036
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J70036/annotation2000051/range2000051/1

https://synbiohub.org/public/igem/BBa_J70036/annotation2000051/range2000051/1
type: Range
persistent identity: range2000051
display id: range2000051
version: 1
top level: BBa_J70036
direction: direction/forward
start: 41
end: 47
orientation: inline

AarI
type: SequenceAnnotation
persistent identity: annotation2000050
display id: annotation2000050
version: 1
top level: BBa_J70036
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J70036/annotation2000050/range2000050/1

https://synbiohub.org/public/igem/BBa_J70036/annotation2000050/range2000050/1
type: Range
persistent identity: range2000050
display id: range2000050
version: 1
top level: BBa_J70036
direction: direction/forward
start: 4
end: 10
orientation: inline

Acc65I
type: ComponentDefinition
persistent identity: BBa M31953
display id: BBa_M31953
version: 1
was generated by: iGEM to SBOL conversion
description: Acc65I restriction enzyme site
created: 2007-02-27T12:00:00Z
modified: 2015-05-08T01:14:01Z
mutable provenance: known sequence
owned by: james
owned by: myers
mutable description: Restriction site
discontinued: false
dominant: true
experience: experience/None
group u list: _102_
m user id: 0
owner id: 1381
owning group id: 102
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: none
star: false
creator: Rebecca Adams
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_M31953_sequence/1

BioScaffol
type: ComponentDefinition
persistent identity: BBa K259002
display id: BBa_K259002
version: 1
was generated by: iGEM to SBOL conversion
description: BioScaffold Linker - Removes Stop Codons & scars & replaces with a Gly-Ser linker
created: 2009-07-31T11:00:00Z
modified: 2015-05-08T01:11:42Z
mutable provenance: This part was designed ''de novo''. The sequence does not occur naturally.
owned by: james
owned by: myers
mutable description: This part is part of a family of Bioscaffold - Linkers. This part will help users interested in protein-fusion. The usage of the part will allow in frame assembly of 2(+) proteins by: *Removing the stop codons from the upstream protein. *Removing the scar created by the Assembly Standard 10 (RFC10) construction of 2(+) protein coding sequences. *Maintaining the start codon of the downstream protein. *Adding a flexible (Gly-Ser-Gly) linker between the two protein coding sequences. *Acting as a translational buffer if ligation is incorrect. ===Assembly Instructions==== *Create an Assembly Standard 10 fusion giving ; **Upstream Protein Part - Bioscaffold Linker - Downstream Protein Part *Use the BioScaffold Specific Enzymes **Restrict with BpuEI to remove upstream scar (created between Upstream Protein - Bioscaffold) and stop codons from upstream protein part. **Ligate and you will get the intermediate product; Upstream Protein (w/out Stop codons) - Tyr - BioScaffold Linker - Downstream Protein **Restrict with BseRI to remove downstream scar. **Ligate to get the final product; Upstream Protein - Tyr-Gly-Ser-Gly- Downstream Protein
discontinued: false
dominant: false
experience: experience/None
group u list: _357_
m user id: 0
owner id: 5318
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: Part was designed taking into account RFC10 assembly standard and as an attempt to be upwards compatible with RFC10.
star: false
creator: Petros Mina
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: Met involved in Restriction/Ligation
sequence annotation: BpuEI Site
sequence annotation: Translational Buffer
sequence annotation: BseRI Site
sequence annotation: Gly-Ser-Gly Linker
sequence annotation: Tyr Residue involved in Restriction/Ligation
sequence: https://synbiohub.org/public/igem/BBa_K259002_sequence/1

Met involved in Restriction/Ligation
type: SequenceAnnotation
persistent identity: annotation2014593
display id: annotation2014593
version: 1
top level: BioScaffol
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K259002/annotation2014593/range2014593/1

https://synbiohub.org/public/igem/BBa_K259002/annotation2014593/range2014593/1
type: Range
persistent identity: range2014593
display id: range2014593
version: 1
top level: BioScaffol
direction: direction/forward
start: 53
end: 55
orientation: inline

BpuEI Site
type: SequenceAnnotation
persistent identity: annotation2014590
display id: annotation2014590
version: 1
top level: BioScaffol
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K259002/annotation2014590/range2014590/1

https://synbiohub.org/public/igem/BBa_K259002/annotation2014590/range2014590/1
type: Range
persistent identity: range2014590
display id: range2014590
version: 1
top level: BioScaffol
direction: direction/reverse
start: 3
end: 8
orientation: inline

Translational Buffer
type: SequenceAnnotation
persistent identity: annotation2014594
display id: annotation2014594
version: 1
top level: BioScaffol
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K259002/annotation2014594/range2014594/1

https://synbiohub.org/public/igem/BBa_K259002/annotation2014594/range2014594/1
type: Range
persistent identity: range2014594
display id: range2014594
version: 1
top level: BioScaffol
direction: direction/forward
start: 56
end: 62
orientation: inline

BseRI Site
type: SequenceAnnotation
persistent identity: annotation2014596
display id: annotation2014596
version: 1
top level: BioScaffol
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K259002/annotation2014596/range2014596/1

https://synbiohub.org/public/igem/BBa_K259002/annotation2014596/range2014596/1
type: Range
persistent identity: range2014596
display id: range2014596
version: 1
top level: BioScaffol
direction: direction/forward
start: 81
end: 86
orientation: inline

Gly-Ser-Gly Linker
type: SequenceAnnotation
persistent identity: annotation2014592
display id: annotation2014592
version: 1
top level: BioScaffol
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K259002/annotation2014592/range2014592/1

https://synbiohub.org/public/igem/BBa_K259002/annotation2014592/range2014592/1
type: Range
persistent identity: range2014592
display id: range2014592
version: 1
top level: BioScaffol
direction: direction/forward
start: 44
end: 52
orientation: inline

Tyr Residue involved in Restriction/Ligation
type: SequenceAnnotation
persistent identity: annotation2014589
display id: annotation2014589
version: 1
top level: BioScaffol
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K259002/annotation2014589/range2014589/1

https://synbiohub.org/public/igem/BBa_K259002/annotation2014589/range2014589/1
type: Range
persistent identity: range2014589
display id: range2014589
version: 1
top level: BioScaffol
direction: direction/forward
start: 41
end: 43
orientation: inline

BseRI Site
type: SequenceAnnotation
persistent identity: annotation2014595
display id: annotation2014595
version: 1
top level: BioScaffol
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K259002/annotation2014595/range2014595/1

https://synbiohub.org/public/igem/BBa_K259002/annotation2014595/range2014595/1
type: Range
persistent identity: range2014595
display id: range2014595
version: 1
top level: BioScaffol
direction: direction/reverse
start: 63
end: 68
orientation: inline

BpuEI Site
type: SequenceAnnotation
persistent identity: annotation2014591
display id: annotation2014591
version: 1
top level: BioScaffol
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K259002/annotation2014591/range2014591/1

https://synbiohub.org/public/igem/BBa_K259002/annotation2014591/range2014591/1
type: Range
persistent identity: range2014591
display id: range2014591
version: 1
top level: BioScaffol
direction: direction/forward
start: 21
end: 26
orientation: inline

RFC 37
type: ComponentDefinition
persistent identity: BBa K245009
display id: BBa_K245009
version: 1
was generated by: iGEM to SBOL conversion
description: RFC 37 Scar Sequence
created: 2009-09-27T11:00:00Z
modified: 2015-05-08T01:11:38Z
mutable provenance: Of synthetic origin.
owned by: james
owned by: myers
mutable description: The sequence codes the scar, that is introduced between two parts, ligated together after being cut with BspEI/NgoMIV.
discontinued: false
dominant: false
experience: experience/Works
group u list: _337_
m user id: 0
owner id: 4969
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: This sequence is introduced as scar as consequence of ligation of two parts, that were previously cut with BspEI/NgoMIV.
star: false
creator: ??pela Miklavič
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: scar SG
sequence: https://synbiohub.org/public/igem/BBa_K245009_sequence/1

scar SG
type: SequenceAnnotation
persistent identity: annotation2027273
display id: annotation2027273
version: 1
top level: RFC 37
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K245009/annotation2027273/range2027273/1

https://synbiohub.org/public/igem/BBa_K245009/annotation2027273/range2027273/1
type: Range
persistent identity: range2027273
display id: range2027273
version: 1
top level: RFC 37
direction: direction/forward
start: 1
end: 6
orientation: inline

6-fingers
type: ComponentDefinition
persistent identity: BBa J35006
display id: BBa_J35006
version: 1
was generated by: iGEM to SBOL conversion
description: 6-fingers arm
created: 2006-10-22T11:00:00Z
modified: 2015-08-31T04:08:47Z
mutable provenance: artificial sequence
owned by: james
owned by: myers
mutable description: DNA ORIGAMI BioBrick
discontinued: false
dominant: false
experience: experience/None
group u list: _64_
m user id: 0
owner id: 807
owning group id: 64
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: OligoAnalyzer 3.0 http://www.idtdna.com/analyzer/Applications/OligoAnalyzer/
star: false
creator: Andrey Kuznetsov
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_J35006_sequence/1

A
type: ComponentDefinition
persistent identity: BBa G0009
display id: BBa_G0009
version: 1
was generated by: iGEM to SBOL conversion
description: The nucleotide A
created: 2007-07-22T11:00:00Z
modified: 2015-08-31T04:07:27Z
mutable provenance: None.
owned by: james
owned by: myers
mutable description: This is a part consisting of only a single nucleotide ... A. It can act as a spacer between restriction sites and parts.
discontinued: false
dominant: false
experience: experience/None
group u list: _41_
m user id: 0
owner id: 126
owning group id: 162
sample status: In stock
status: status/Available
bookmark: false
mutable notes: None.
star: false
creator: Reshma Shetty
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_G0009_sequence/1

BBa_J36859
type: ComponentDefinition
persistent identity: BBa J36859
display id: BBa_J36859
version: 1
was generated by: iGEM to SBOL conversion
description: A15 adaptamer
created: 2006-10-28T11:00:00Z
modified: 2015-08-31T04:08:47Z
mutable provenance: synthetic
owned by: james
owned by: myers
mutable description: A15 is a single-piece adaptamer which which can physically link together the proteins thrombin and streptavidin.
discontinued: false
dominant: false
experience: experience/None
group u list: _65_
m user id: 0
owner id: 1245
owning group id: 65
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable notes: Linking region had to avoid disruption of aptamer secondary structure.
star: false
creator: Lewis Hahn
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_J36859_sequence/1

BBa_J36855
type: ComponentDefinition
persistent identity: BBa J36855
display id: BBa_J36855
version: 1
was generated by: iGEM to SBOL conversion
description: T20 linkaptamer
created: 2006-10-28T11:00:00Z
modified: 2015-08-31T04:08:47Z
mutable provenance: synthetic
owned by: james
owned by: myers
mutable description: T20 is the thrombin-binding component of the A35 adaptamer which can physically link together the proteins thrombin and streptavidin. A20 contains a 15 bp double-stranded linking region between thrombin and streptavidin aptamer sequences.
discontinued: false
dominant: false
experience: experience/None
group u list: _65_
m user id: 0
owner id: 1245
owning group id: 65
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable notes: Linking region had to avoid disruption of aptamer secondary structure.
star: false
creator: Lewis Hahn
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_J36855_sequence/1

IscA1
type: ComponentDefinition
persistent identity: BBa M36907
display id: BBa_M36907
version: 1
was generated by: iGEM to SBOL conversion
description: Coding Sequence of IscA1 pigeon homologue (codon-optimized for humans)
creator: Michael Becich, Thomas Lau, Sreyas Misra
created: 2015-10-24T11:00:00Z
modified: 2015-12-04T07:45:15Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _848_
m user id: 29123
owner id: 29123
owning group id: 9
status: status/Planning
bookmark: true
mutable description: The gene Iron-Sulfur cluster assembly 1 (IscA1) is a mitochondrial protein involved in the biogenesis and assembly of iron-sulfur clusters, which play a role in electron-transfer reactions and is theorized to be associated with certain organisms' magnetic perception. The homing pigeon is one such organism with this ability, and here we have codon-optimized the Columba Livia homologue for homo sapiens. The sequence can be inserted into plasmids for expression of the magnetoreceptor IscA gene, which hopefully will create magnetogenetic biosensors in the form of iron-sulfur cluster, for downstream output and applications.
mutable notes: While the plasmid design for this insert can be complicated, the coding sequence itself is quite simple. We only needed to remove the start codon in order to co-express a fluorescent protein, and modify two BbsII restriction enzyme sites by altering the third base pair of codons (wobble effect) at bp locations 75 (C->G) and 309 (A->G).
mutable provenance: The predicted IscA amino acid sequence was obtained from NCBI (http://www.ncbi.nlm.nih.gov/protein/XP_005508102.1), and codon-optimized for humans with IDT's tools.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_M36907_sequence/1

BBa_K1680014
type: ComponentDefinition
persistent identity: BBa K1680014
display id: BBa_K1680014
version: 1
was generated by: iGEM to SBOL conversion
description: pRS313 yeast shuttle vector with Biobrick MCS
creator: Katharina Sporbeck
created: 2015-09-16T11:00:00Z
modified: 2015-10-27T01:47:18Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2098_
m user id: 15693
owner id: 26460
owning group id: 9
status: status/Planning
bookmark: false
mutable description: A shuttle vector for yeast. The vector is compatible with RFC10 standard and contains an ADH terminator behind the RFC10 cloning site. Ampicilin resistance for bacteria and URA3 gene for yeast selection.
mutable notes: contains an ADH Terminator and restrictions sites for the RFC10 standard only in the multiple cloning site, no restrictions sites for RCF10 enzymes in the backbone.
mutable provenance: yeast shuttle vector
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: RFC10 MCS
sequence annotation: HIS3
sequence annotation: T3 promotor
sequence annotation: ampR promotor
sequence annotation: CEN/ARS yeast centromere
sequence annotation: pUC origin
sequence annotation: T7 promotor
sequence annotation: lac promotor
sequence annotation: lacZalpha
sequence annotation: ampR
sequence annotation: HIS3 promotor
sequence annotation: f1 ori
sequence: https://synbiohub.org/public/igem/BBa_K1680014_sequence/1

RFC10 MCS
type: SequenceAnnotation
persistent identity: annotation2466135
display id: annotation2466135
version: 1
top level: BBa_K1680014
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1680014/annotation2466135/range2466135/1

https://synbiohub.org/public/igem/BBa_K1680014/annotation2466135/range2466135/1
type: Range
persistent identity: range2466135
display id: range2466135
version: 1
direction: direction/forward
top level: BBa_K1680014
start: 2083
end: 2125
orientation: inline

HIS3
type: SequenceAnnotation
persistent identity: annotation2466098
display id: annotation2466098
version: 1
top level: BBa_K1680014
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1680014/annotation2466098/range2466098/1

https://synbiohub.org/public/igem/BBa_K1680014/annotation2466098/range2466098/1
type: Range
persistent identity: range2466098
display id: range2466098
version: 1
direction: direction/forward
top level: BBa_K1680014
start: 503
end: 1162
orientation: inline

T3 promotor
type: SequenceAnnotation
persistent identity: annotation2466125
display id: annotation2466125
version: 1
top level: BBa_K1680014
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1680014/annotation2466125/range2466125/1

https://synbiohub.org/public/igem/BBa_K1680014/annotation2466125/range2466125/1
type: Range
persistent identity: range2466125
display id: range2466125
version: 1
direction: direction/reverse
top level: BBa_K1680014
start: 2153
end: 2172
orientation: inline

ampR promotor
type: SequenceAnnotation
persistent identity: annotation2466130
display id: annotation2466130
version: 1
top level: BBa_K1680014
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1680014/annotation2466130/range2466130/1

https://synbiohub.org/public/igem/BBa_K1680014/annotation2466130/range2466130/1
type: Range
persistent identity: range2466130
display id: range2466130
version: 1
direction: direction/reverse
top level: BBa_K1680014
start: 4215
end: 4319
orientation: inline

CEN/ARS yeast centromere
type: SequenceAnnotation
persistent identity: annotation2466131
display id: annotation2466131
version: 1
top level: BBa_K1680014
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1680014/annotation2466131/range2466131/1

https://synbiohub.org/public/igem/BBa_K1680014/annotation2466131/range2466131/1
type: Range
persistent identity: range2466131
display id: range2466131
version: 1
direction: direction/forward
top level: BBa_K1680014
start: 4356
end: 4859
orientation: inline

pUC origin
type: SequenceAnnotation
persistent identity: annotation2466128
display id: annotation2466128
version: 1
top level: BBa_K1680014
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1680014/annotation2466128/range2466128/1

https://synbiohub.org/public/igem/BBa_K1680014/annotation2466128/range2466128/1
type: Range
persistent identity: range2466128
display id: range2466128
version: 1
direction: direction/reverse
top level: BBa_K1680014
start: 2595
end: 3183
orientation: inline

T7 promotor
type: SequenceAnnotation
persistent identity: annotation2466133
display id: annotation2466133
version: 1
top level: BBa_K1680014
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1680014/annotation2466133/range2466133/1

https://synbiohub.org/public/igem/BBa_K1680014/annotation2466133/range2466133/1
type: Range
persistent identity: range2466133
display id: range2466133
version: 1
direction: direction/forward
top level: BBa_K1680014
start: 2046
end: 2065
orientation: inline

lac promotor
type: SequenceAnnotation
persistent identity: annotation2466127
display id: annotation2466127
version: 1
top level: BBa_K1680014
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1680014/annotation2466127/range2466127/1

https://synbiohub.org/public/igem/BBa_K1680014/annotation2466127/range2466127/1
type: Range
persistent identity: range2466127
display id: range2466127
version: 1
direction: direction/reverse
top level: BBa_K1680014
start: 2241
end: 2271
orientation: inline

lacZalpha
type: SequenceAnnotation
persistent identity: annotation2466100
display id: annotation2466100
version: 1
top level: BBa_K1680014
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1680014/annotation2466100/range2466100/1

https://synbiohub.org/public/igem/BBa_K1680014/annotation2466100/range2466100/1
type: Range
persistent identity: range2466100
display id: range2466100
version: 1
direction: direction/forward
top level: BBa_K1680014
start: 1661
end: 2077
orientation: inline

ampR
type: SequenceAnnotation
persistent identity: annotation2466129
display id: annotation2466129
version: 1
top level: BBa_K1680014
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1680014/annotation2466129/range2466129/1

https://synbiohub.org/public/igem/BBa_K1680014/annotation2466129/range2466129/1
type: Range
persistent identity: range2466129
display id: range2466129
version: 1
direction: direction/reverse
top level: BBa_K1680014
start: 3354
end: 4214
orientation: inline

HIS3 promotor
type: SequenceAnnotation
persistent identity: annotation2466090
display id: annotation2466090
version: 1
top level: BBa_K1680014
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1680014/annotation2466090/range2466090/1

https://synbiohub.org/public/igem/BBa_K1680014/annotation2466090/range2466090/1
type: Range
persistent identity: range2466090
display id: range2466090
version: 1
direction: direction/forward
top level: BBa_K1680014
start: 315
end: 502
orientation: inline

f1 ori
type: SequenceAnnotation
persistent identity: annotation2466099
display id: annotation2466099
version: 1
top level: BBa_K1680014
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1680014/annotation2466099/range2466099/1

https://synbiohub.org/public/igem/BBa_K1680014/annotation2466099/range2466099/1
type: Range
persistent identity: range2466099
display id: range2466099
version: 1
direction: direction/reverse
top level: BBa_K1680014
start: 1423
end: 1878
orientation: inline

BclI
type: ComponentDefinition
persistent identity: BBa M31965
display id: BBa_M31965
version: 1
was generated by: iGEM to SBOL conversion
description: BclI restriction enzyme site
created: 2007-02-27T12:00:00Z
modified: 2015-05-08T01:14:01Z
mutable provenance: known sequence
owned by: james
owned by: myers
mutable description: restriction site
discontinued: false
dominant: true
experience: experience/None
group u list: _102_
m user id: 0
owner id: 1381
owning group id: 102
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: none
star: false
creator: Rebecca Adams
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_M31965_sequence/1

NotI
type: ComponentDefinition
persistent identity: BBa M31982
display id: BBa_M31982
version: 1
was generated by: iGEM to SBOL conversion
description: NotI restriction enzyme site
created: 2007-02-28T12:00:00Z
modified: 2015-05-08T01:14:01Z
mutable provenance: none
owned by: james
owned by: myers
mutable description: NotI restriction enzyme site
discontinued: false
dominant: false
experience: experience/None
group u list: _102_
m user id: 0
owner id: 1373
owning group id: 102
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: none
star: false
creator: Tiffany Guo
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_M31982_sequence/1

SalI
type: ComponentDefinition
persistent identity: BBa I11077
display id: BBa_I11077
version: 1
was generated by: iGEM to SBOL conversion
description: SalI restriction enzyme site with extra flanking nucleotides
created: 2004-09-06T11:00:00Z
modified: 2015-08-31T04:07:30Z
mutable provenance: NEB catalog
owned by: james
owned by: myers
mutable description: SalI restriction site
discontinued: false
dominant: false
experience: experience/None
group u list: _2_
m user id: 0
owner id: 102
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: bu
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_I11077_sequence/1

PspOMI
type: ComponentDefinition
persistent identity: BBa M31984
display id: BBa_M31984
version: 1
was generated by: iGEM to SBOL conversion
description: PspOMI restriction enzyme site
created: 2007-02-28T12:00:00Z
modified: 2015-05-08T01:14:01Z
mutable provenance: none
owned by: james
owned by: myers
mutable description: PspOMI restriction enzyme site
discontinued: false
dominant: false
experience: experience/None
group u list: _102_
m user id: 0
owner id: 1373
owning group id: 102
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: none
star: false
creator: Tiffany Guo
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_M31984_sequence/1

BBa_K1930002
type: ComponentDefinition
persistent identity: BBa K1930002
display id: BBa_K1930002
version: 1
was generated by: iGEM to SBOL conversion
description: Encryption key sequence in integration plasmid (BBa_K823023)
created: 2016-10-15T11:00:00Z
modified: 2016-10-17T09:00:28Z
mutable provenance: Key sequence: Created by our CryptoGErM software. http://2016.igem.org/Team:Groningen/Encoding BBa_K823023: Created by iGEM Munich 2012. http://parts.igem.org/Part:BBa_K823023
owned by: james
owned by: myers
mutable description: The iGEM team Groningen 2016 worked on bioencryption in order to safely store data in DNA. In our project we were mainly working with two sequences of DNA which were the encrypted message sequence and the encryption key sequence. The key sequence was created by our software. It is therefore artificial DNA. We ordered the encrypted key sequence as gBlock from IDT (Integrated DNA technologies). This part was created in the interest of having a genomic integration plasmid with our key sequence. We used an available BioBrick BBa_K823023 from igem Munich 2012. It is an integration plasmid for Bacillus subtilis, which can be used for cloning in E. coli as well. An RFP is inserted in BBa_K823023 for more efficient screening after transformation.
discontinued: true
dominant: false
experience: experience/Works
group u list: _2397_
m user id: 29748
owner id: 29748
owning group id: 9
status: status/Deleted
bookmark: false
mutable notes: The iGEM team Groningen 2016 worked on bioencryption in order to safely store data in DNA. In our project we were mainly working with two sequences of DNA which were the encrypted message sequence and the encryption key sequence. The key sequence was created by our software. It is therefore artificial DNA. We ordered the encrypted key sequence as gBlock from IDT (Integrated DNA technologies). This part was created in the interest of having a genomic integration plasmid with our key sequence. We used an available BioBrick BBa_K823023 from igem Munich 2012. It is an integration plasmid for Bacillus subtilis, which can be used for cloning in E. coli as well. An RFP is inserted in BBa_K823023 for more efficient screening after transformation.
star: false
creator: Eike Mahlandt
type: DnaRegion
role: SO:0000155
role: partType/Plasmid
sequence annotation: amyE
sequence annotation: bla
sequence annotation: key sequence
sequence annotation: BBa_K823023
sequence annotation: BBa_K1930000
sequence annotation: cat
sequence: https://synbiohub.org/public/igem/BBa_K1930002_sequence/1

amyE
type: SequenceAnnotation
persistent identity: annotation2514194
display id: annotation2514194
version: 1
top level: BBa_K1930002
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1930002/annotation2514194/range2514194/1

https://synbiohub.org/public/igem/BBa_K1930002/annotation2514194/range2514194/1
type: Range
persistent identity: range2514194
display id: range2514194
version: 1
top level: BBa_K1930002
direction: direction/forward
start: 5525
end: 6045
orientation: inline

bla
type: SequenceAnnotation
persistent identity: annotation2514193
display id: annotation2514193
version: 1
top level: BBa_K1930002
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1930002/annotation2514193/range2514193/1

https://synbiohub.org/public/igem/BBa_K1930002/annotation2514193/range2514193/1
type: Range
persistent identity: range2514193
display id: range2514193
version: 1
top level: BBa_K1930002
direction: direction/forward
start: 4340
end: 5200
orientation: inline

key sequence
type: SequenceAnnotation
persistent identity: annotation2514195
display id: annotation2514195
version: 1
top level: BBa_K1930002
role: feature/dna
role: SO:0000110
location: https://synbiohub.org/public/igem/BBa_K1930002/annotation2514195/range2514195/1

https://synbiohub.org/public/igem/BBa_K1930002/annotation2514195/range2514195/1
type: Range
persistent identity: range2514195
display id: range2514195
version: 1
top level: BBa_K1930002
direction: direction/forward
start: 6063
end: 6206
orientation: inline

amyE
type: SequenceAnnotation
persistent identity: annotation2514192
display id: annotation2514192
version: 1
top level: BBa_K1930002
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1930002/annotation2514192/range2514192/1

https://synbiohub.org/public/igem/BBa_K1930002/annotation2514192/range2514192/1
type: Range
persistent identity: range2514192
display id: range2514192
version: 1
top level: BBa_K1930002
direction: direction/forward
start: 1358
end: 2386
orientation: inline

BBa_K823023
type: SequenceAnnotation
persistent identity: annotation2514190
display id: annotation2514190
version: 1
top level: BBa_K1930002
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1930002/annotation2514190/range2514190/1

https://synbiohub.org/public/igem/BBa_K1930002/annotation2514190/range2514190/1
type: Range
persistent identity: range2514190
display id: range2514190
version: 1
top level: BBa_K1930002
direction: direction/forward
start: 1
end: 6062
orientation: inline

BBa_K1930000
type: SequenceAnnotation
persistent identity: annotation2514196
display id: annotation2514196
version: 1
top level: BBa_K1930002
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1930002/annotation2514196/range2514196/1

https://synbiohub.org/public/igem/BBa_K1930002/annotation2514196/range2514196/1
type: Range
persistent identity: range2514196
display id: range2514196
version: 1
top level: BBa_K1930002
direction: direction/forward
start: 6063
end: 6206
orientation: inline

cat
type: SequenceAnnotation
persistent identity: annotation2514191
display id: annotation2514191
version: 1
top level: BBa_K1930002
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1930002/annotation2514191/range2514191/1

https://synbiohub.org/public/igem/BBa_K1930002/annotation2514191/range2514191/1
type: Range
persistent identity: range2514191
display id: range2514191
version: 1
top level: BBa_K1930002
direction: direction/forward
start: 337
end: 987
orientation: inline

BBa_M34014
type: ComponentDefinition
persistent identity: BBa M34014
display id: BBa_M34014
version: 1
was generated by: iGEM to SBOL conversion
description: Nucleotide sequence of a cDNA clone of saxiphilin from the liver of Rana Catesbeiana
created: 2011-05-04T11:00:00Z
modified: 2015-05-08T01:14:02Z
mutable provenance: It comes from the cDNA corresponding to the RNA of the bullfrog (Rana Catesbeiana) liver.
owned by: myers
owned by: james
mutable description: This is the nucleotide sequence of a cDNA clone of saxiphilin from the liver of the American Bullfrog, Rana Catesbeiana. Saxiphilin is a protein that binds a neurotoxin called saxitoxin. It is a non-membrane bound binding site and is considered to be a member of the transferrin family of plasma proteins.
discontinued: false
dominant: false
group u list: _838_
m user id: 0
owner id: 9446
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Since it is a copy of a cDNA derived from RNA that is already spliced, the sequence only reflects the exon parts, not the introns.
star: false
creator: Kristine Kim
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_M34014_sequence/1

BBa_K175001
type: ComponentDefinition
persistent identity: BBa K175001
display id: BBa_K175001
version: 1
was generated by: iGEM to SBOL conversion
description: trbK (entry exclusion) of IncP beta R751 plasmid
created: 2009-07-05T11:00:00Z
modified: 2015-05-08T01:10:59Z
mutable provenance: This sequence can be found at http://www.ncbi.nlm.nih.gov/protein/10955220
owned by: james
owned by: myers
mutable description: This part encodes for trbK, a small 75 amino acid cytoplasmic membrane lipoprotein which handles the entry exclusion in the R751 plasmid. Expression of this part in a cell without R751 will significantly reduce the transfer frequency from R751 donors. This is designed to prevent redundant transfers from occurring. Note that the presence of entry exclusion has been linked to the prevention of lethal zygosis. If the entry exclusion (Eex) index is defined as the transfer frequency for receiver cells not containing the trbK protein divided by the transfer frequency for receiver cells containing the trbK protein than for R751 Eex index = 44,000 (See Table 5 of Haase, J., Kalkum, M. & Lanka, E. TrbK, a small cytoplasmic membrane lipoprotein, functions in entry exclusion of the IncP alpha plasmid RP4. J. Bacteriol. 178, 6720???6729 (1996).[http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=178567])
discontinued: false
dominant: false
experience: experience/Works
group u list: _280_
m user id: 0
owner id: 4286
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: None
star: false
creator: Calin Plesa
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: trbK
sequence: https://synbiohub.org/public/igem/BBa_K175001_sequence/1

trbK
type: SequenceAnnotation
persistent identity: annotation2009448
display id: annotation2009448
version: 1
top level: BBa_K175001
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K175001/annotation2009448/range2009448/1

https://synbiohub.org/public/igem/BBa_K175001/annotation2009448/range2009448/1
type: Range
persistent identity: range2009448
display id: range2009448
version: 1
top level: BBa_K175001
direction: direction/forward
start: 1
end: 231
orientation: inline

scar
type: ComponentDefinition
persistent identity: BBa G0000
display id: BBa_G0000
version: 1
was generated by: iGEM to SBOL conversion
description: SpeI/XbaI scar for RBS-CDS junctions
created: 2007-07-22T11:00:00Z
modified: 2015-08-31T04:07:27Z
mutable provenance: SpeI/XbaI scar
owned by: james
owned by: myers
mutable description: This is the sequence of the SpeI/XbaI scar for RBS-CDS junctions in BioBricks standard assembly.
discontinued: false
dominant: true
experience: experience/None
group u list: _41_
m user id: 0
owner id: 126
owning group id: 162
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: This is a shorter scar to ensure proper spacing between the RBS and CDS.
star: false
creator: Reshma Shetty
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_G0000_sequence/1

Acc651
type: ComponentDefinition
persistent identity: BBa M31997
display id: BBa_M31997
version: 1
was generated by: iGEM to SBOL conversion
description: Acc65I restriction enzyme site
created: 2007-02-27T12:00:00Z
modified: 2015-05-08T01:14:01Z
mutable provenance: An E. coli strain that carries the cloned Acc65I gene from Acinetobacter calcoaceticus 65
owned by: james
owned by: myers
mutable description: Acc65I restriction enzyme site
discontinued: false
dominant: true
experience: experience/None
group u list: _102_
m user id: 0
owner id: 1373
owning group id: 102
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: none
star: false
creator: Tiffany Guo
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_M31997_sequence/1

OriT-RP4
type: ComponentDefinition
persistent identity: BBa K125320
display id: BBa_K125320
version: 1
was generated by: iGEM to SBOL conversion
description: OriT-RP4 (Origin of transfer for the RP4 plasmid)
created: 2008-08-28T11:00:00Z
modified: 2015-05-08T01:09:45Z
mutable provenance: RP4 NCBI accession number NC_001621
owned by: james
owned by: myers
mutable description: Relaxation region of self-transmissible plasmid RP4. When this part is added to a plasmid, it is transferred by RP4 through conjugation.
discontinued: false
dominant: false
experience: experience/None
group u list: _179_
m user id: 0
owner id: 3138
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: This part is similar to J01003, which has no experience and is much longer. In the process of designing and testing this part, it will be compared to J01003.
star: false
creator: Margaret Ruzicka
type: DnaRegion
role: partType/Conjugation
role: SO:0000724
sequence: https://synbiohub.org/public/igem/BBa_K125320_sequence/1

BBa_J36854
type: ComponentDefinition
persistent identity: BBa J36854
display id: BBa_J36854
version: 1
was generated by: iGEM to SBOL conversion
description: T35 linkaptamer
created: 2006-10-28T11:00:00Z
modified: 2015-08-31T04:08:47Z
mutable provenance: synthetic
owned by: james
owned by: myers
mutable description: T35 is the thrombin-binding component of the A35 adaptamer which can physically link together the proteins thrombin and streptavidin.
discontinued: false
dominant: false
experience: experience/None
group u list: _65_
m user id: 0
owner id: 1245
owning group id: 65
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Linking region had to avoid disruption of aptamer secondary structure.
star: false
creator: Lewis Hahn
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_J36854_sequence/1

IscA2
type: ComponentDefinition
persistent identity: BBa M36908
display id: BBa_M36908
version: 1
was generated by: iGEM to SBOL conversion
description: Coding Sequence of IscA2 pigeon homologue (codon-optimized for humans)
creator: Michael Becich
created: 2015-10-24T11:00:00Z
modified: 2015-12-04T08:06:13Z
discontinued: false
dominant: false
experience: experience/Fails
group u list: _848_
m user id: 29123
owner id: 29123
owning group id: 9
status: status/Planning
bookmark: false
mutable description: The gene Iron-Sulfur cluster assembly 2 (IscA2) is a mitochondrial protein involved in the biogenesis and assembly of iron-sulfur clusters, which play a role in electron-transfer reactions and is theorized to be associated with certain organisms' magnetic perception. The homing pigeon is one such organism with this ability, and here we have codon-optimized the Columba Livia homologue for homo sapiens. The sequence can be inserted into plasmids for expression of the magnetoreceptor IscA gene, which hopefully will create magnetogenetic biosensors in the form of iron-sulfur cluster, for downstream output and applications.
mutable notes: While the plasmid design for this insert can be complicated, the coding sequence itself is quite simple. We only needed to add the start codon since this second isoform did not have methionine built in.
mutable provenance: The predicted IscA amino acid sequence was obtained from NCBI (http://www.ncbi.nlm.nih.gov/protein/XP_005502393.1), and codon-optimized for humans with IDT's tools.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316

O1
type: ComponentDefinition
persistent identity: BBa K2054001
display id: BBa_K2054001
version: 1
was generated by: iGEM to SBOL conversion
description: Oligo 1
creator: LAI HEI WAI
created: 2016-10-13T11:00:00Z
modified: 2016-10-27T11:55:37Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2522_
m user id: 30316
owner id: 30316
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This will be use to build our desired nanostructure. This ssDNA contains also split G-quadruplex sequences that allow the oligo itself to function as an DNAzyme under specific conditions.
mutable notes: Strand Displacement
mutable provenance: Plasmid Construction: Elbaz, J., Yin, P., & Voigt, C. A. (2016). Genetic encoding of DNA nanostructures and their self-assembly in living bacteria. Nature communications, 7. Oligo Construction: Nakatsuka, K., Shigeto, H., Kuroda, A., & Funabashi, H. (2015). A split G-quadruplex-based DNA nano-tweezers structure as a signal-transducing molecule for the homogeneous detection of specific nucleic acids. Biosensors and Bioelectronics, 74, 222-226.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_K2054001_sequence/1

BBa_M1000
type: ComponentDefinition
persistent identity: BBa M1000
display id: BBa_M1000
version: 1
was generated by: iGEM to SBOL conversion
description: Left mosaic end for Tn5 transpososome
creator: Tom Knight
created: 2006-03-16T12:00:00Z
modified: 2015-05-08T01:13:51Z
discontinued: true
dominant: false
experience: experience/None
group u list: _41_48_1_
m user id: 0
owner id: 6
owning group id: 48
sample status: Discontinued
status: status/Deleted
bookmark: false
mutable description: Left end of a linear transpososome sequence containing the 19 bp mosaic end in the forward orientation. The sequence also contains a PvuII cut site which releases the transpososome when cut with PvuII. Constructed compound parts intended for transposition must not contain other PvuII end, or be PCR amplified with blunt end producing enzymes (Pfu, Phusion) at the PvuII cut site between CAG and CTG. This part, and the companion part M1001 provide Biobrick tools to construct Tn5 transpososomes: linear DNA sequences which can be cut in vitro from a plasmid backbone, or created by PCR, combined in vitro with purified Tn5 trasposase protein, electroportated into cells, and finally randomly inserting into the bacterial chromosome. The parts assembled between the two mosaic ends can be delivered, including antibiotic resistance markers or other functional chromosomal insertions.
mutable notes: ;biobrick left end, TA cloning overhang GTT TCT TCG AAT TCG CGG CCG CTT CTA GAG ;biobrick right end, TA cloning overhang GTT TCT TCC TGC AGC GGC CGC TAC TAG TA ;mosaic end with cag upstream to insert PvuII cut site cagCTGTCTCTTATACACATCT ;mosaic end reverse complement with ctg upstream to insert PvuII cut site AGATGTGTATAAGAGACAGctg ;mosaic left end in biobrick form construction primer F GTT TCT TCG AAT TCG CGG CCG CTT CTA GAG cagCTGTCTCTTATACACATCT, MEL-F ;mosaic left end in biobrick form construction primer R GTT TCT TCC TGC AGC GGC CGC TAC TAG TA AGATGTGTATAAGAGACAGctg, MEL-R ;mosaic right end in biobrick form construction primer F GTT TCT TCG AAT TCG CGG CCG CTT CTA GAG AGATGTGTATAAGAGACAGctg, MER-F ;mosaic right end in biobrick form construction primer R GTT TCT TCC TGC AGC GGC CGC TAC TAG TA cagCTGTCTCTTATACACATCT, MER-R
mutable provenance: Synthesis from overlapping oligos
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: Mosaic End (forward)
sequence annotation: PvuII cut site
sequence: https://synbiohub.org/public/igem/BBa_M1000_sequence/1

Mosaic End (forward)
type: SequenceAnnotation
persistent identity: annotation1825569
display id: annotation1825569
version: 1
top level: BBa_M1000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_M1000/annotation1825569/range1825569/1

https://synbiohub.org/public/igem/BBa_M1000/annotation1825569/range1825569/1
type: Range
persistent identity: range1825569
display id: range1825569
version: 1
direction: direction/forward
top level: BBa_M1000
start: 4
end: 22
orientation: inline

PvuII cut site
type: SequenceAnnotation
persistent identity: annotation1825568
display id: annotation1825568
version: 1
top level: BBa_M1000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_M1000/annotation1825568/range1825568/1

https://synbiohub.org/public/igem/BBa_M1000/annotation1825568/range1825568/1
type: Range
persistent identity: range1825568
display id: range1825568
version: 1
direction: direction/forward
top level: BBa_M1000
start: 1
end: 6
orientation: inline

EcoRI
type: ComponentDefinition
persistent identity: BBa M31983
display id: BBa_M31983
version: 1
was generated by: iGEM to SBOL conversion
description: EcoRI restriction enzyme site
created: 2007-02-28T12:00:00Z
modified: 2015-05-08T01:14:01Z
mutable provenance: none
owned by: james
owned by: myers
mutable description: EcoRI restriction enzyme site
discontinued: false
dominant: true
experience: experience/None
group u list: _102_
m user id: 0
owner id: 1373
owning group id: 102
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: none
star: false
creator: Tiffany Guo
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_M31983_sequence/1

BBa_J70010
type: ComponentDefinition
persistent identity: BBa J70010
display id: BBa_J70010
version: 1
was generated by: iGEM to SBOL conversion
description: A BioScaffold Part (Uses PpiI), for offset see Part Design page
created: 2008-05-08T11:00:00Z
modified: 2015-05-08T01:08:20Z
mutable provenance: designed
owned by: james
owned by: myers
mutable description: still in testing
discontinued: false
dominant: false
experience: experience/None
group u list: _41_
m user id: 0
owner id: 1201
owning group id: 41
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: still in testing
star: false
creator: Julie Norville
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: PpiI2
sequence annotation: PpiI 1
sequence annotation: PacI 2
sequence annotation: MabI 1
sequence annotation: MabI 2
sequence annotation: PacI 1
sequence annotation: PpiI 2
sequence annotation: PacI3
sequence: https://synbiohub.org/public/igem/BBa_J70010_sequence/1

PpiI2
type: SequenceAnnotation
persistent identity: annotation1963029
display id: annotation1963029
version: 1
top level: BBa_J70010
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J70010/annotation1963029/range1963029/1

https://synbiohub.org/public/igem/BBa_J70010/annotation1963029/range1963029/1
type: Range
persistent identity: range1963029
display id: range1963029
version: 1
top level: BBa_J70010
direction: direction/forward
start: 51
end: 53
orientation: inline

PpiI 1
type: SequenceAnnotation
persistent identity: annotation1963026
display id: annotation1963026
version: 1
top level: BBa_J70010
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J70010/annotation1963026/range1963026/1

https://synbiohub.org/public/igem/BBa_J70010/annotation1963026/range1963026/1
type: Range
persistent identity: range1963026
display id: range1963026
version: 1
top level: BBa_J70010
direction: direction/forward
start: 1
end: 3
orientation: inline

PacI 2
type: SequenceAnnotation
persistent identity: annotation1963033
display id: annotation1963033
version: 1
top level: BBa_J70010
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_J70010/annotation1963033/range1963033/1

https://synbiohub.org/public/igem/BBa_J70010/annotation1963033/range1963033/1
type: Range
persistent identity: range1963033
display id: range1963033
version: 1
top level: BBa_J70010
direction: direction/forward
start: 27
end: 34
orientation: inline

MabI 1
type: SequenceAnnotation
persistent identity: annotation1963030
display id: annotation1963030
version: 1
top level: BBa_J70010
role: feature/dna
role: SO:0000110
location: https://synbiohub.org/public/igem/BBa_J70010/annotation1963030/range1963030/1

https://synbiohub.org/public/igem/BBa_J70010/annotation1963030/range1963030/1
type: Range
persistent identity: range1963030
display id: range1963030
version: 1
top level: BBa_J70010
direction: direction/forward
start: 13
end: 19
orientation: inline

MabI 2
type: SequenceAnnotation
persistent identity: annotation1963031
display id: annotation1963031
version: 1
top level: BBa_J70010
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_J70010/annotation1963031/range1963031/1

https://synbiohub.org/public/igem/BBa_J70010/annotation1963031/range1963031/1
type: Range
persistent identity: range1963031
display id: range1963031
version: 1
top level: BBa_J70010
direction: direction/forward
start: 35
end: 41
orientation: inline

PpiI 1
type: SequenceAnnotation
persistent identity: annotation1963027
display id: annotation1963027
version: 1
top level: BBa_J70010
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J70010/annotation1963027/range1963027/1

https://synbiohub.org/public/igem/BBa_J70010/annotation1963027/range1963027/1
type: Range
persistent identity: range1963027
display id: range1963027
version: 1
top level: BBa_J70010
direction: direction/forward
start: 9
end: 12
orientation: inline

PacI 1
type: SequenceAnnotation
persistent identity: annotation1963032
display id: annotation1963032
version: 1
top level: BBa_J70010
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_J70010/annotation1963032/range1963032/1

https://synbiohub.org/public/igem/BBa_J70010/annotation1963032/range1963032/1
type: Range
persistent identity: range1963032
display id: range1963032
version: 1
top level: BBa_J70010
direction: direction/forward
start: 19
end: 24
orientation: inline

PpiI 2
type: SequenceAnnotation
persistent identity: annotation1963028
display id: annotation1963028
version: 1
top level: BBa_J70010
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J70010/annotation1963028/range1963028/1

https://synbiohub.org/public/igem/BBa_J70010/annotation1963028/range1963028/1
type: Range
persistent identity: range1963028
display id: range1963028
version: 1
top level: BBa_J70010
direction: direction/forward
start: 42
end: 45
orientation: inline

PacI3
type: SequenceAnnotation
persistent identity: annotation1963034
display id: annotation1963034
version: 1
top level: BBa_J70010
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_J70010/annotation1963034/range1963034/1

https://synbiohub.org/public/igem/BBa_J70010/annotation1963034/range1963034/1
type: Range
persistent identity: range1963034
display id: range1963034
version: 1
top level: BBa_J70010
direction: direction/forward
start: 23
end: 30
orientation: inline

scar
type: ComponentDefinition
persistent identity: BBa G0004
display id: BBa_G0004
version: 1
was generated by: iGEM to SBOL conversion
description: SpeI/NheI mixed site
created: 2007-02-26T12:00:00Z
modified: 2015-08-31T04:07:27Z
mutable provenance: NheI and SpeI
owned by: james
owned by: myers
mutable description: NheI/SpeI mixed site. Used to help enter parts into the registry.
discontinued: false
dominant: false
experience: experience/None
group u list: _41_
m user id: 0
owner id: 126
owning group id: 70
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: None.
star: false
creator: Reshma Shetty
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_G0004_sequence/1

BBa_K1930003
type: ComponentDefinition
persistent identity: BBa K1930003
display id: BBa_K1930003
version: 1
was generated by: iGEM to SBOL conversion
description: Encrypted message sequence in integration plasmid (BBa_K823023)
created: 2016-10-15T11:00:00Z
modified: 2016-10-17T09:01:43Z
mutable provenance: Message sequence: Created by our CryptoGErM software. http://2016.igem.org/Team:Groningen/Encoding BBa_K823023: Created by iGEM Munich 2012. http://parts.igem.org/Part:BBa_K823023
owned by: james
owned by: myers
mutable description: The iGEM team Groningen 2016 worked on bioencryption in order to safely store data in DNA. In our project we were mainly working with two sequences of DNA which were the encrypted message sequence and the encryption key sequence. The message sequence was created by our software. It is therefore artificial DNA. We ordered the encrypted message sequence as gBlock from IDT (Integrated DNA technologies). This part was created in the interest of having a genomic integration plasmid with our message sequence. We used an available BioBrick BBa_K823023 from igem Munich 2012. It is an integration plasmid for Bacillus subtilis, which can be used for cloning in E. coli as well. An RFP is inserted in BBa_K823023 for more efficient screening after transformation.
discontinued: true
dominant: false
experience: experience/Works
group u list: _2397_
m user id: 29748
owner id: 29748
owning group id: 9
status: status/Deleted
bookmark: false
mutable notes: The iGEM team Groningen 2016 worked on bioencryption in order to safely store data in DNA. In our project we were mainly working with two sequences of DNA which were the encrypted message sequence and the encryption key sequence. The message sequence was created by our software. It is therefore artificial DNA. We ordered the encrypted message sequence as gBlock from IDT (Integrated DNA technologies). This part was created in the interest of having a genomic integration plasmid with our message sequence. We used an available BioBrick BBa_K823023 from igem Munich 2012. It is an integration plasmid for Bacillus subtilis, which can be used for cloning in E. coli as well. An RFP is inserted in BBa_K823023 for more efficient screening after transformation. For more information see plasmid construction under our lab journal: http://2016.igem.org/Team:Groningen/Experiments
star: false
creator: Barbora Waclawikova
type: DnaRegion
role: SO:0000155
role: partType/Plasmid
sequence annotation: cat
sequence annotation: amyE
sequence annotation: BBa_K1930001
sequence annotation: BBa_K823023
sequence annotation: bla
sequence annotation: message sequence
sequence: https://synbiohub.org/public/igem/BBa_K1930003_sequence/1

cat
type: SequenceAnnotation
persistent identity: annotation2513446
display id: annotation2513446
version: 1
top level: BBa_K1930003
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1930003/annotation2513446/range2513446/1

https://synbiohub.org/public/igem/BBa_K1930003/annotation2513446/range2513446/1
type: Range
persistent identity: range2513446
display id: range2513446
version: 1
top level: BBa_K1930003
direction: direction/forward
start: 893
end: 1543
orientation: inline

amyE
type: SequenceAnnotation
persistent identity: annotation2513447
display id: annotation2513447
version: 1
top level: BBa_K1930003
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1930003/annotation2513447/range2513447/1

https://synbiohub.org/public/igem/BBa_K1930003/annotation2513447/range2513447/1
type: Range
persistent identity: range2513447
display id: range2513447
version: 1
top level: BBa_K1930003
direction: direction/forward
start: 1914
end: 2942
orientation: inline

BBa_K1930001
type: SequenceAnnotation
persistent identity: annotation2514197
display id: annotation2514197
version: 1
top level: BBa_K1930003
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1930003/annotation2514197/range2514197/1

https://synbiohub.org/public/igem/BBa_K1930003/annotation2514197/range2514197/1
type: Range
persistent identity: range2514197
display id: range2514197
version: 1
top level: BBa_K1930003
direction: direction/forward
start: 1
end: 556
orientation: inline

BBa_K823023
type: SequenceAnnotation
persistent identity: annotation2513444
display id: annotation2513444
version: 1
top level: BBa_K1930003
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1930003/annotation2513444/range2513444/1

https://synbiohub.org/public/igem/BBa_K1930003/annotation2513444/range2513444/1
type: Range
persistent identity: range2513444
display id: range2513444
version: 1
top level: BBa_K1930003
direction: direction/forward
start: 557
end: 6618
orientation: inline

bla
type: SequenceAnnotation
persistent identity: annotation2513448
display id: annotation2513448
version: 1
top level: BBa_K1930003
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1930003/annotation2513448/range2513448/1

https://synbiohub.org/public/igem/BBa_K1930003/annotation2513448/range2513448/1
type: Range
persistent identity: range2513448
display id: range2513448
version: 1
top level: BBa_K1930003
direction: direction/forward
start: 4896
end: 5756
orientation: inline

amyE
type: SequenceAnnotation
persistent identity: annotation2513449
display id: annotation2513449
version: 1
top level: BBa_K1930003
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1930003/annotation2513449/range2513449/1

https://synbiohub.org/public/igem/BBa_K1930003/annotation2513449/range2513449/1
type: Range
persistent identity: range2513449
display id: range2513449
version: 1
top level: BBa_K1930003
direction: direction/forward
start: 6081
end: 6601
orientation: inline

message sequence
type: SequenceAnnotation
persistent identity: annotation2513445
display id: annotation2513445
version: 1
top level: BBa_K1930003
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1930003/annotation2513445/range2513445/1

https://synbiohub.org/public/igem/BBa_K1930003/annotation2513445/range2513445/1
type: Range
persistent identity: range2513445
display id: range2513445
version: 1
top level: BBa_K1930003
direction: direction/forward
start: 1
end: 556
orientation: inline

BamHI rs
type: ComponentDefinition
persistent identity: BBa K792013
display id: BBa_K792013
version: 1
was generated by: iGEM to SBOL conversion
description: BamHI restriction site (standalone)
created: 2012-09-24T11:00:00Z
modified: 2015-05-08T01:13:23Z
mutable provenance: None
owned by: james
owned by: myers
mutable description: This is a '''BamHI'''restriction site. This part might not be useful as a physical biobrick. It has been created to follow modularization in parts X
discontinued: false
dominant: false
experience: experience/None
group u list: _1047_
m user id: 0
owner id: 11811
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: None
star: false
creator: Manuel Gim??nez
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: BamHI restriction site
sequence: https://synbiohub.org/public/igem/BBa_K792013_sequence/1

BamHI restriction site
type: SequenceAnnotation
persistent identity: annotation2197287
display id: annotation2197287
version: 1
top level: BamHI rs
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K792013/annotation2197287/range2197287/1

https://synbiohub.org/public/igem/BBa_K792013/annotation2197287/range2197287/1
type: Range
persistent identity: range2197287
display id: range2197287
version: 1
top level: BamHI rs
direction: direction/forward
start: 1
end: 6
orientation: inline

AatII
type: ComponentDefinition
persistent identity: BBa M31952
display id: BBa_M31952
version: 1
was generated by: iGEM to SBOL conversion
description: AatII restriction enzyme site
created: 2007-02-27T12:00:00Z
modified: 2015-05-08T01:14:01Z
mutable provenance: known it sequence
owned by: james
owned by: myers
mutable description: restriction site
discontinued: false
dominant: false
experience: experience/None
group u list: _102_
m user id: 0
owner id: 1381
owning group id: 102
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: none
star: false
creator: Rebecca Adams
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_M31952_sequence/1

AvrII
type: ComponentDefinition
persistent identity: BBa B0048
display id: BBa_B0048
version: 1
was generated by: iGEM to SBOL conversion
description: AvrII restriction enzyme site (XbaI, SpeI compatible overhang)
created: 2006-03-13T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: AvrII recognition site. Digestion with AvrII generates a compatible cohesive end to XbaI and SpeI.
discontinued: false
dominant: true
experience: experience/None
group u list: _41_6_
m user id: 0
owner id: 126
owning group id: 45
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Reshma Shetty
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: AvrII site
sequence: https://synbiohub.org/public/igem/BBa_B0048_sequence/1

AvrII site
type: SequenceAnnotation
persistent identity: annotation1822744
display id: annotation1822744
version: 1
top level: AvrII
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_B0048/annotation1822744/range1822744/1

https://synbiohub.org/public/igem/BBa_B0048/annotation1822744/range1822744/1
type: Range
persistent identity: range1822744
display id: range1822744
version: 1
top level: AvrII
direction: direction/forward
start: 1
end: 6
orientation: inline

BBa_J33207
type: ComponentDefinition
persistent identity: BBa J33207
display id: BBa_J33207
version: 1
was generated by: iGEM to SBOL conversion
description: lac promoter and lacZ
created: 2006-10-26T11:00:00Z
modified: 2015-08-31T04:08:46Z
mutable provenance: The DNA was amplified from E. coli BL21 genomic DNA using primers based on published sequence (Genbank accession J01636, gi:146575). The annotation shown here is based on that associated with this Genbank entry. The sequence shown here is derived by sequencing the construct.
owned by: james
owned by: myers
mutable description: This part (submitted in pSB1A2) consists of the lac promoter and lacZ' gene, encoding the N-terminal 76 amino acid residues of LacZ, sufficient to complement the lacZ-delta-M15 mutation for blue-white selection on Xgal plates. A SacI site has been introduced at the 3' end, overlapping the XbaI site of the Biobrick prefix. This is designed to be used as a cloning vector for making new biobricks. PCR primers can be designed with a SacI site in one primer and an SpeI site in the other. This removes the necessity for an excessively long non-complementary tail on one primer bearing either the full biobrick prefix or suffix. The PCR product can then be digested with SacI and SpeI for insertion into this plasmid, replacing the Plac-lacZ' cassette. Recombinant plasmids will then be white on IPTG/Xgal plates, whereas any that still contain the original insert will be blue. We have used this strategy to prepare several biobricks, including BBa_J33204, which contains the xylE gene encoding catechol-2,3-dioxygenase. (For making biobricks that contain lacZ', BBa_J33204 can be used in the same way; in this case, clones with plasmids that still contain xylE will turn yellow on addition of a drop of 10 mM catechol.)
discontinued: false
dominant: false
experience: experience/Works
group u list: _63_
m user id: 0
owner id: 837
owning group id: 63
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: Note that the SacI site overlaps the SpeI site. The Biobrick prefix ends ...TCTAGAG. When this is added to the CTC at the start of the sequence shown here, the SacI site, GAGCTC, is generated.
star: false
creator: Chris French
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: rbs
sequence annotation: SacI
sequence annotation: lacZ'
sequence annotation: -35
sequence annotation: CAP binding site
sequence annotation: -10
sequence annotation: LacI binding site
sequence: https://synbiohub.org/public/igem/BBa_J33207_sequence/1

rbs
type: SequenceAnnotation
persistent identity: annotation1907857
display id: annotation1907857
version: 1
top level: BBa_J33207
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_J33207/annotation1907857/range1907857/1

https://synbiohub.org/public/igem/BBa_J33207/annotation1907857/range1907857/1
type: Range
persistent identity: range1907857
display id: range1907857
version: 1
top level: BBa_J33207
direction: direction/forward
start: 359
end: 362
orientation: inline

SacI
type: SequenceAnnotation
persistent identity: annotation1907854
display id: annotation1907854
version: 1
top level: BBa_J33207
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J33207/annotation1907854/range1907854/1

https://synbiohub.org/public/igem/BBa_J33207/annotation1907854/range1907854/1
type: Range
persistent identity: range1907854
display id: range1907854
version: 1
top level: BBa_J33207
direction: direction/forward
start: 1
end: 3
orientation: inline

lacZ'
type: SequenceAnnotation
persistent identity: annotation1907858
display id: annotation1907858
version: 1
top level: BBa_J33207
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J33207/annotation1907858/range1907858/1

https://synbiohub.org/public/igem/BBa_J33207/annotation1907858/range1907858/1
type: Range
persistent identity: range1907858
display id: range1907858
version: 1
top level: BBa_J33207
direction: direction/forward
start: 370
end: 600
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1907860
display id: annotation1907860
version: 1
top level: BBa_J33207
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_J33207/annotation1907860/range1907860/1

https://synbiohub.org/public/igem/BBa_J33207/annotation1907860/range1907860/1
type: Range
persistent identity: range1907860
display id: range1907860
version: 1
top level: BBa_J33207
direction: direction/forward
start: 297
end: 302
orientation: inline

CAP binding site
type: SequenceAnnotation
persistent identity: annotation1907855
display id: annotation1907855
version: 1
top level: BBa_J33207
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_J33207/annotation1907855/range1907855/1

https://synbiohub.org/public/igem/BBa_J33207/annotation1907855/range1907855/1
type: Range
persistent identity: range1907855
display id: range1907855
version: 1
top level: BBa_J33207
direction: direction/forward
start: 248
end: 285
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1907859
display id: annotation1907859
version: 1
top level: BBa_J33207
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_J33207/annotation1907859/range1907859/1

https://synbiohub.org/public/igem/BBa_J33207/annotation1907859/range1907859/1
type: Range
persistent identity: range1907859
display id: range1907859
version: 1
top level: BBa_J33207
direction: direction/forward
start: 320
end: 325
orientation: inline

LacI binding site
type: SequenceAnnotation
persistent identity: annotation1907856
display id: annotation1907856
version: 1
top level: BBa_J33207
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_J33207/annotation1907856/range1907856/1

https://synbiohub.org/public/igem/BBa_J33207/annotation1907856/range1907856/1
type: Range
persistent identity: range1907856
display id: range1907856
version: 1
top level: BBa_J33207
direction: direction/forward
start: 332
end: 352
orientation: inline

BBa_I714030
type: ComponentDefinition
persistent identity: BBa I714030
display id: BBa_I714030
version: 1
was generated by: iGEM to SBOL conversion
description: OriT-F (Origin of Transfer for the F-plasmid nic region)
created: 2007-10-19T11:00:00Z
modified: 2015-08-31T04:07:47Z
mutable provenance: Origin-of-transfer nic region excised from Plasmid F genomic DNA.(NCBI:AP001918)
owned by: james
owned by: myers
mutable description: OriT-F (Origin of Transfer for the F-type conjugative plasmid) is the nic region, where the relaxosome nicks the plasmid and conjugative transfer by F plasmid machinery begins via rolling circle replication.
discontinued: false
dominant: false
experience: experience/Works
group u list: _142_
m user id: 0
owner id: 2248
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: This OriT-F is the same as the host cell's native Origin of Transfer, Please compare the sequence with J01002.
star: true
creator: Mingzhi Qu
type: DnaRegion
role: partType/Conjugation
role: SO:0000724
sequence: https://synbiohub.org/public/igem/BBa_I714030_sequence/1

AscI
type: ComponentDefinition
persistent identity: BBa M31990
display id: BBa_M31990
version: 1
was generated by: iGEM to SBOL conversion
description: AscI restriction enzyme site
created: 2007-02-28T12:00:00Z
modified: 2015-05-08T01:14:01Z
mutable provenance: none
owned by: james
owned by: myers
mutable description: AscI restriction enzyme site
discontinued: false
dominant: false
experience: experience/None
group u list: _102_
m user id: 0
owner id: 1373
owning group id: 102
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: none
star: false
creator: Tiffany Guo
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_M31990_sequence/1

AvrII/XbaI
type: ComponentDefinition
persistent identity: BBa B0104
display id: BBa_B0104
version: 1
was generated by: iGEM to SBOL conversion
description: AvrII/XbaI mixed site
created: 2007-03-06T12:00:00Z
modified: 2015-08-31T04:07:21Z
mutable provenance: n/a
owned by: myers
owned by: james
mutable description: This is the 6 bp mixed site that results when ligating the compatible ends (CTAG 5' overhang) that result from AvrII cleavage (CCTAGA) of the upstream part and XbaI (TCTAGA) cleavage of the downstream part.
discontinued: false
dominant: false
experience: experience/None
group u list: _11_
m user id: 0
owner id: 571
owning group id: 10
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: n/a
star: false
creator: Heather Keller
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: AvrII/XbaI mixed site
sequence: https://synbiohub.org/public/igem/BBa_B0104_sequence/1

AvrII/XbaI mixed site
type: SequenceAnnotation
persistent identity: annotation1920669
display id: annotation1920669
version: 1
top level: AvrII/XbaI
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_B0104/annotation1920669/range1920669/1

https://synbiohub.org/public/igem/BBa_B0104/annotation1920669/range1920669/1
type: Range
persistent identity: range1920669
display id: range1920669
version: 1
top level: AvrII/XbaI
direction: direction/forward
start: 1
end: 6
orientation: inline

BBa_J70012
type: ComponentDefinition
persistent identity: BBa J70012
display id: BBa_J70012
version: 1
was generated by: iGEM to SBOL conversion
description: A BioScaffold Part (Uses PsrI), Protein Head Remover see Part Design page
created: 2008-05-08T11:00:00Z
modified: 2015-05-08T01:08:20Z
mutable provenance: designed
owned by: james
owned by: myers
mutable description: still in testing
discontinued: false
dominant: false
experience: experience/None
group u list: _41_
m user id: 0
owner id: 1201
owning group id: 41
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: still in testing
star: false
creator: Julie Norville
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: Mab 2
sequence annotation: AscI 1
sequence annotation: Mab 1
sequence annotation: PsrI 1
sequence annotation: PsrI 2
sequence annotation: AscI 2
sequence: https://synbiohub.org/public/igem/BBa_J70012_sequence/1

Mab 2
type: SequenceAnnotation
persistent identity: annotation1963020
display id: annotation1963020
version: 1
top level: BBa_J70012
role: feature/dna
role: SO:0000110
location: https://synbiohub.org/public/igem/BBa_J70012/annotation1963020/range1963020/1

https://synbiohub.org/public/igem/BBa_J70012/annotation1963020/range1963020/1
type: Range
persistent identity: range1963020
display id: range1963020
version: 1
top level: BBa_J70012
direction: direction/forward
start: 47
end: 52
orientation: inline

AscI 1
type: SequenceAnnotation
persistent identity: annotation1963021
display id: annotation1963021
version: 1
top level: BBa_J70012
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_J70012/annotation1963021/range1963021/1

https://synbiohub.org/public/igem/BBa_J70012/annotation1963021/range1963021/1
type: Range
persistent identity: range1963021
display id: range1963021
version: 1
top level: BBa_J70012
direction: direction/forward
start: 20
end: 27
orientation: inline

Mab 1
type: SequenceAnnotation
persistent identity: annotation1963018
display id: annotation1963018
version: 1
top level: BBa_J70012
role: feature/dna
role: SO:0000110
location: https://synbiohub.org/public/igem/BBa_J70012/annotation1963018/range1963018/1

https://synbiohub.org/public/igem/BBa_J70012/annotation1963018/range1963018/1
type: Range
persistent identity: range1963018
display id: range1963018
version: 1
top level: BBa_J70012
direction: direction/forward
start: 4
end: 10
orientation: inline

PsrI 1
type: SequenceAnnotation
persistent identity: annotation1963015
display id: annotation1963015
version: 1
top level: BBa_J70012
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J70012/annotation1963015/range1963015/1

https://synbiohub.org/public/igem/BBa_J70012/annotation1963015/range1963015/1
type: Range
persistent identity: range1963015
display id: range1963015
version: 1
top level: BBa_J70012
direction: direction/forward
start: 1
end: 3
orientation: inline

PsrI 2
type: SequenceAnnotation
persistent identity: annotation1963017
display id: annotation1963017
version: 1
top level: BBa_J70012
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J70012/annotation1963017/range1963017/1

https://synbiohub.org/public/igem/BBa_J70012/annotation1963017/range1963017/1
type: Range
persistent identity: range1963017
display id: range1963017
version: 1
top level: BBa_J70012
direction: direction/forward
start: 43
end: 46
orientation: inline

AscI 2
type: SequenceAnnotation
persistent identity: annotation1963022
display id: annotation1963022
version: 1
top level: BBa_J70012
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_J70012/annotation1963022/range1963022/1

https://synbiohub.org/public/igem/BBa_J70012/annotation1963022/range1963022/1
type: Range
persistent identity: range1963022
display id: range1963022
version: 1
top level: BBa_J70012
direction: direction/forward
start: 28
end: 34
orientation: inline

PsrI 1
type: SequenceAnnotation
persistent identity: annotation1963016
display id: annotation1963016
version: 1
top level: BBa_J70012
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J70012/annotation1963016/range1963016/1

https://synbiohub.org/public/igem/BBa_J70012/annotation1963016/range1963016/1
type: Range
persistent identity: range1963016
display id: range1963016
version: 1
top level: BBa_J70012
direction: direction/forward
start: 10
end: 12
orientation: inline

BBa_K649204
type: ComponentDefinition
persistent identity: BBa K649204
display id: BBa_K649204
version: 1
was generated by: iGEM to SBOL conversion
description: lox2272
created: 2011-09-24T11:00:00Z
modified: 2015-05-08T01:13:00Z
mutable provenance: lox2272
owned by: james
owned by: myers
mutable description: lox2272
discontinued: false
dominant: false
experience: experience/Works
group u list: _826_
m user id: 0
owner id: 8230
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: lox2272
star: false
creator: SeoHyun Kim
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: lox2272
sequence: https://synbiohub.org/public/igem/BBa_K649204_sequence/1

lox2272
type: SequenceAnnotation
persistent identity: annotation2139901
display id: annotation2139901
version: 1
top level: BBa_K649204
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K649204/annotation2139901/range2139901/1

https://synbiohub.org/public/igem/BBa_K649204/annotation2139901/range2139901/1
type: Range
persistent identity: range2139901
display id: range2139901
version: 1
top level: BBa_K649204
direction: direction/forward
start: 1
end: 34
orientation: inline

BBa_K1479005
type: ComponentDefinition
persistent identity: BBa K1479005
display id: BBa_K1479005
version: 1
was generated by: iGEM to SBOL conversion
created: 2014-10-05T11:00:00Z
modified: 2015-05-08T01:10:40Z
mutable provenance: NULL
owned by: james
owned by: myers
mutable description: This part is just for upload test. It is meaningless
discontinued: false
dominant: false
experience: experience/None
group u list: _1859_
m user id: 0
owner id: 21515
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: NULL
star: false
type: DnaRegion
role: partType/Promoter
sequence annotation: cds
sequence annotation: 1
sequence annotation: st
sequence: https://synbiohub.org/public/igem/BBa_K1479005_sequence/1

cds
type: SequenceAnnotation
persistent identity: annotation2417782
display id: annotation2417782
version: 1
top level: BBa_K1479005
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1479005/annotation2417782/range2417782/1

https://synbiohub.org/public/igem/BBa_K1479005/annotation2417782/range2417782/1
type: Range
persistent identity: range2417782
display id: range2417782
version: 1
top level: BBa_K1479005
direction: direction/forward
start: 3
end: 5
orientation: inline

1
type: SequenceAnnotation
persistent identity: annotation2417781
display id: annotation2417781
version: 1
top level: BBa_K1479005
role: feature/start
role: SO:0000318
location: https://synbiohub.org/public/igem/BBa_K1479005/annotation2417781/range2417781/1

https://synbiohub.org/public/igem/BBa_K1479005/annotation2417781/range2417781/1
type: Range
persistent identity: range2417781
display id: range2417781
version: 1
top level: BBa_K1479005
direction: direction/forward
start: 1
end: 2
orientation: inline

st
type: SequenceAnnotation
persistent identity: annotation2417783
display id: annotation2417783
version: 1
top level: BBa_K1479005
role: feature/stop
role: SO:0000319
location: https://synbiohub.org/public/igem/BBa_K1479005/annotation2417783/range2417783/1

https://synbiohub.org/public/igem/BBa_K1479005/annotation2417783/range2417783/1
type: Range
persistent identity: range2417783
display id: range2417783
version: 1
top level: BBa_K1479005
direction: direction/reverse
start: 6
end: 7
orientation: inline

XbaI
type: ComponentDefinition
persistent identity: BBa I11082
display id: BBa_I11082
version: 1
was generated by: iGEM to SBOL conversion
description: XbaI restriction site with extra flanking nucleotides
created: 2004-09-06T11:00:00Z
modified: 2015-08-31T04:07:30Z
mutable provenance: NEB catalogue
owned by: james
owned by: myers
mutable description: XbaI restriction site
discontinued: false
dominant: false
experience: experience/None
group u list: _2_
m user id: 0
owner id: 102
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: bu
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_I11082_sequence/1

MfeI
type: ComponentDefinition
persistent identity: BBa B0047
display id: BBa_B0047
version: 1
was generated by: iGEM to SBOL conversion
description: MfeI restriction enzyme site (EcoRI compatible overhang)
created: 2006-03-13T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: MfeI recognition site. Digestion with MfeI yields a compatible cohesive end to EcoRI.
discontinued: false
dominant: true
experience: experience/None
group u list: _41_6_
m user id: 0
owner id: 126
owning group id: 45
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Reshma Shetty
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: MfeI site
sequence: https://synbiohub.org/public/igem/BBa_B0047_sequence/1

MfeI site
type: SequenceAnnotation
persistent identity: annotation1822740
display id: annotation1822740
version: 1
top level: MfeI
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_B0047/annotation1822740/range1822740/1

https://synbiohub.org/public/igem/BBa_B0047/annotation1822740/range1822740/1
type: Range
persistent identity: range1822740
display id: range1822740
version: 1
top level: MfeI
direction: direction/forward
start: 1
end: 6
orientation: inline

PvuI
type: ComponentDefinition
persistent identity: BBa I11073
display id: BBa_I11073
version: 1
was generated by: iGEM to SBOL conversion
description: PvuI restriction enzyme site with extra flanking nucleotides
created: 2004-09-06T11:00:00Z
modified: 2015-08-31T04:07:30Z
mutable provenance: NEB catalogue
owned by: james
owned by: myers
mutable description: PvuI restriction site with 3 flanking base pairs
discontinued: false
dominant: false
experience: experience/None
group u list: _2_
m user id: 0
owner id: 102
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: bu
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: PvuI
sequence: https://synbiohub.org/public/igem/BBa_I11073_sequence/1

PvuI
type: SequenceAnnotation
persistent identity: annotation1148272
display id: annotation1148272
version: 1
top level: PvuI
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I11073/annotation1148272/range1148272/1

https://synbiohub.org/public/igem/BBa_I11073/annotation1148272/range1148272/1
type: Range
persistent identity: range1148272
display id: range1148272
version: 1
top level: PvuI
direction: direction/forward
start: 4
end: 9
orientation: inline

BssHII
type: ComponentDefinition
persistent identity: BBa M31989
display id: BBa_M31989
version: 1
was generated by: iGEM to SBOL conversion
description: BssHII restriction enzyme site
created: 2007-02-28T12:00:00Z
modified: 2015-05-08T01:14:01Z
mutable provenance: none
owned by: james
owned by: myers
mutable description: BssHII restriction enzyme site
discontinued: false
dominant: true
experience: experience/None
group u list: _102_
m user id: 0
owner id: 1373
owning group id: 102
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: none
star: false
creator: Tiffany Guo
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_M31989_sequence/1

BfuAI
type: ComponentDefinition
persistent identity: BBa M31968
display id: BBa_M31968
version: 1
was generated by: iGEM to SBOL conversion
description: BfuAI restriction enzyme site
created: 2007-02-27T12:00:00Z
modified: 2015-05-08T01:14:01Z
mutable provenance: known sequence
owned by: james
owned by: myers
mutable description: restriction site
discontinued: false
dominant: false
experience: experience/None
group u list: _102_
m user id: 0
owner id: 1381
owning group id: 102
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: none
star: false
creator: Rebecca Adams
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_M31968_sequence/1

BBa_J70031
type: ComponentDefinition
persistent identity: BBa J70031
display id: BBa_J70031
version: 1
was generated by: iGEM to SBOL conversion
description: Linker for BioScaffold Parts, example of Gamma BioScaffold part
created: 2008-12-12T12:00:00Z
modified: 2015-05-08T01:08:20Z
mutable provenance: designed
owned by: james
owned by: myers
mutable description: Will use as a linker when fusing two BioScaffold parts, can be created as an oligo
discontinued: false
dominant: false
experience: experience/None
group u list: _41_
m user id: 0
owner id: 1201
owning group id: 41
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: needs to be longer than 28 bp
star: false
creator: Julie Norville
type: DnaRegion
role: SO:0000110
role: partType/Temporary
sequence annotation: BBa Scar
sequence annotation: MabI
sequence: https://synbiohub.org/public/igem/BBa_J70031_sequence/1

BBa Scar
type: SequenceAnnotation
persistent identity: annotation2000306
display id: annotation2000306
version: 1
top level: BBa_J70031
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_J70031/annotation2000306/range2000306/1

https://synbiohub.org/public/igem/BBa_J70031/annotation2000306/range2000306/1
type: Range
persistent identity: range2000306
display id: range2000306
version: 1
top level: BBa_J70031
direction: direction/forward
start: 8
end: 15
orientation: inline

MabI
type: SequenceAnnotation
persistent identity: annotation2000305
display id: annotation2000305
version: 1
top level: BBa_J70031
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_J70031/annotation2000305/range2000305/1

https://synbiohub.org/public/igem/BBa_J70031/annotation2000305/range2000305/1
type: Range
persistent identity: range2000305
display id: range2000305
version: 1
top level: BBa_J70031
direction: direction/forward
start: 16
end: 22
orientation: inline

MabI
type: SequenceAnnotation
persistent identity: annotation2000304
display id: annotation2000304
version: 1
top level: BBa_J70031
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_J70031/annotation2000304/range2000304/1

https://synbiohub.org/public/igem/BBa_J70031/annotation2000304/range2000304/1
type: Range
persistent identity: range2000304
display id: range2000304
version: 1
top level: BBa_J70031
direction: direction/forward
start: 1
end: 7
orientation: inline

BBa_K510000
type: ComponentDefinition
persistent identity: BBa K510000
display id: BBa_K510000
version: 1
was generated by: iGEM to SBOL conversion
description: pUC18Sfi-miniTn7BB-Gm
created: 2011-09-15T11:00:00Z
modified: 2015-05-08T01:12:30Z
mutable provenance: The pUC18-SfiI cloning vector was kindly provided by the Microbiology laboratory of Centro Andaluz de Biolog??a del Desarrollo (CABD) in Sevilla. The miniTn7BB-Gm synthetic transposon was designed by the iGEM 2011 team UPO-Sevilla based on the set of miniTn7 derivatives developed by Choi et al. (2005) and commercially synthesized by Mr. Gene (Regensburg, Germany). K.-H. Choi, J. B. Gaynor, K. G. White, C. Lopez, C. M. Bosio, R. R. Karkhoff-Schweizer & H. P. Schweizer (2005). A Tn7-based broad-range bacterial cloning and expression system. Nature Methods, vol.2 NO.6, June 2005, 443.
owned by: james
owned by: myers
mutable description: The miniTn7BB-Gm synthetic minitransposon was digested at the flanking SfiI sites and cloned into SfiI-digested pUC18-Sfi. This results in the elimination of the complete pUC18-SfiI multi-cloning site, except for the duplicated SfiI sites that remain on both sides of the transposon, thus facilitating its transfer to other vectors. This plasmid can be used to keep the mini-Tn7-Gm in E. coli and other enterics, and may be used for transposition into the genomes of non-enteric bacteria, in which it is non-replicative. The structure of the mini-Tn7BB-Gm transposon is shown in the figure below. Tn7R and Tn7L are the right and left ends of Tn7 transposon, respectively, required for recognition of the transposase machinery and transposition. FRT is the Flp recombinase target site for excision of the resistance once the transposon is inserted in the genome of a living organism. The gentamycin resistance cassette (Gm) is flanked by restriction sites in order to facilitate the generation of variants with different antibiotic resistance markers. The BioBrick cloning site (BCS) includes the prefix and suffix restrictions sites used in assembly standard 10, and it is the place where BioBricks are inserted for genome integration. The complete construction is flanked by SfiI restriction sites to facilitate cloning.
discontinued: false
dominant: false
experience: experience/Works
group u list: _673_
m user id: 0
owner id: 6157
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: In order to make this vector compatible with assembly standard 10, several restriction sites were removed from the synthetic sequence.
star: false
creator: David Caballero, Fernando Govantes
type: DnaRegion
role: SO:0000155
role: partType/Plasmid
sequence annotation: ColEI (pUC18) Replication origin
sequence annotation: Prefix
sequence annotation: SfiI restriction site
sequence annotation: SphI restriction site
sequence annotation: Tn7-L end
sequence annotation: Flipase recombination sequence (FRT)
sequence annotation: NcoI restriction site
sequence annotation: Ampicillin resistance (bla)
sequence annotation: NcoI restrictino site
sequence annotation: Terminator
sequence annotation: VR (BBa_G00101) primer binding site
sequence annotation: Tn7-R end
sequence annotation: Suffix
sequence annotation: double terminator (BBa_B0014)
sequence annotation: Gentamycin resistance
sequence annotation: VF2 (G00100) primer binding site
sequence annotation: E. coli his operon terminator
sequence: https://synbiohub.org/public/igem/BBa_K510000_sequence/1

ColEI (pUC18) Replication origin
type: SequenceAnnotation
persistent identity: annotation2137417
display id: annotation2137417
version: 1
top level: BBa_K510000
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K510000/annotation2137417/range2137417/1

https://synbiohub.org/public/igem/BBa_K510000/annotation2137417/range2137417/1
type: Range
persistent identity: range2137417
display id: range2137417
version: 1
top level: BBa_K510000
direction: direction/forward
start: 1965
end: 2644
orientation: inline

Prefix
type: SequenceAnnotation
persistent identity: annotation2137423
display id: annotation2137423
version: 1
top level: BBa_K510000
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K510000/annotation2137423/range2137423/1

https://synbiohub.org/public/igem/BBa_K510000/annotation2137423/range2137423/1
type: Range
persistent identity: range2137423
display id: range2137423
version: 1
top level: BBa_K510000
direction: direction/forward
start: 4367
end: 4387
orientation: inline

SfiI restriction site
type: SequenceAnnotation
persistent identity: annotation2137415
display id: annotation2137415
version: 1
top level: BBa_K510000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K510000/annotation2137415/range2137415/1

https://synbiohub.org/public/igem/BBa_K510000/annotation2137415/range2137415/1
type: Range
persistent identity: range2137415
display id: range2137415
version: 1
top level: BBa_K510000
direction: direction/forward
start: 348
end: 360
orientation: inline

SphI restriction site
type: SequenceAnnotation
persistent identity: annotation2137425
display id: annotation2137425
version: 1
top level: BBa_K510000
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K510000/annotation2137425/range2137425/1

https://synbiohub.org/public/igem/BBa_K510000/annotation2137425/range2137425/1
type: Range
persistent identity: range2137425
display id: range2137425
version: 1
top level: BBa_K510000
direction: direction/forward
start: 3381
end: 3386
orientation: inline

SfiI restriction site
type: SequenceAnnotation
persistent identity: annotation2137418
display id: annotation2137418
version: 1
top level: BBa_K510000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K510000/annotation2137418/range2137418/1

https://synbiohub.org/public/igem/BBa_K510000/annotation2137418/range2137418/1
type: Range
persistent identity: range2137418
display id: range2137418
version: 1
top level: BBa_K510000
direction: direction/forward
start: 3002
end: 3014
orientation: inline

Tn7-L end
type: SequenceAnnotation
persistent identity: annotation2137414
display id: annotation2137414
version: 1
top level: BBa_K510000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K510000/annotation2137414/range2137414/1

https://synbiohub.org/public/igem/BBa_K510000/annotation2137414/range2137414/1
type: Range
persistent identity: range2137414
display id: range2137414
version: 1
top level: BBa_K510000
direction: direction/forward
start: 182
end: 347
orientation: inline

SphI restriction site
type: SequenceAnnotation
persistent identity: annotation2137440
display id: annotation2137440
version: 1
top level: BBa_K510000
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K510000/annotation2137440/range2137440/1

https://synbiohub.org/public/igem/BBa_K510000/annotation2137440/range2137440/1
type: Range
persistent identity: range2137440
display id: range2137440
version: 1
top level: BBa_K510000
direction: direction/forward
start: 4190
end: 4195
orientation: inline

Flipase recombination sequence (FRT)
type: SequenceAnnotation
persistent identity: annotation2137422
display id: annotation2137422
version: 1
top level: BBa_K510000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K510000/annotation2137422/range2137422/1

https://synbiohub.org/public/igem/BBa_K510000/annotation2137422/range2137422/1
type: Range
persistent identity: range2137422
display id: range2137422
version: 1
top level: BBa_K510000
direction: direction/forward
start: 4196
end: 4243
orientation: inline

NcoI restriction site
type: SequenceAnnotation
persistent identity: annotation2137439
display id: annotation2137439
version: 1
top level: BBa_K510000
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K510000/annotation2137439/range2137439/1

https://synbiohub.org/public/igem/BBa_K510000/annotation2137439/range2137439/1
type: Range
persistent identity: range2137439
display id: range2137439
version: 1
top level: BBa_K510000
direction: direction/forward
start: 4184
end: 4189
orientation: inline

Ampicillin resistance (bla)
type: SequenceAnnotation
persistent identity: annotation2137416
display id: annotation2137416
version: 1
top level: BBa_K510000
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K510000/annotation2137416/range2137416/1

https://synbiohub.org/public/igem/BBa_K510000/annotation2137416/range2137416/1
type: Range
persistent identity: range2137416
display id: range2137416
version: 1
top level: BBa_K510000
direction: direction/forward
start: 966
end: 1826
orientation: inline

Flipase recombination sequence (FRT)
type: SequenceAnnotation
persistent identity: annotation2137420
display id: annotation2137420
version: 1
top level: BBa_K510000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K510000/annotation2137420/range2137420/1

https://synbiohub.org/public/igem/BBa_K510000/annotation2137420/range2137420/1
type: Range
persistent identity: range2137420
display id: range2137420
version: 1
top level: BBa_K510000
direction: direction/forward
start: 3329
end: 3376
orientation: inline

NcoI restrictino site
type: SequenceAnnotation
persistent identity: annotation2137436
display id: annotation2137436
version: 1
top level: BBa_K510000
role: feature/dna
role: SO:0000110
location: https://synbiohub.org/public/igem/BBa_K510000/annotation2137436/range2137436/1

https://synbiohub.org/public/igem/BBa_K510000/annotation2137436/range2137436/1
type: Range
persistent identity: range2137436
display id: range2137436
version: 1
top level: BBa_K510000
direction: direction/forward
start: 3387
end: 3392
orientation: inline

Terminator
type: SequenceAnnotation
persistent identity: annotation2137449
display id: annotation2137449
version: 1
top level: BBa_K510000
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K510000/annotation2137449/range2137449/1

https://synbiohub.org/public/igem/BBa_K510000/annotation2137449/range2137449/1
type: Range
persistent identity: range2137449
display id: range2137449
version: 1
top level: BBa_K510000
direction: direction/forward
start: 4326
end: 4359
orientation: inline

VR (BBa_G00101) primer binding site
type: SequenceAnnotation
persistent identity: annotation2137413
display id: annotation2137413
version: 1
top level: BBa_K510000
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K510000/annotation2137413/range2137413/1

https://synbiohub.org/public/igem/BBa_K510000/annotation2137413/range2137413/1
type: Range
persistent identity: range2137413
display id: range2137413
version: 1
top level: BBa_K510000
direction: direction/forward
start: 157
end: 176
orientation: inline

Tn7-R end
type: SequenceAnnotation
persistent identity: annotation2137419
display id: annotation2137419
version: 1
top level: BBa_K510000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K510000/annotation2137419/range2137419/1

https://synbiohub.org/public/igem/BBa_K510000/annotation2137419/range2137419/1
type: Range
persistent identity: range2137419
display id: range2137419
version: 1
top level: BBa_K510000
direction: direction/forward
start: 3014
end: 3213
orientation: inline

Suffix
type: SequenceAnnotation
persistent identity: annotation2137411
display id: annotation2137411
version: 1
top level: BBa_K510000
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K510000/annotation2137411/range2137411/1

https://synbiohub.org/public/igem/BBa_K510000/annotation2137411/range2137411/1
type: Range
persistent identity: range2137411
display id: range2137411
version: 1
top level: BBa_K510000
direction: direction/forward
start: 2
end: 21
orientation: inline

double terminator (BBa_B0014)
type: SequenceAnnotation
persistent identity: annotation2137424
display id: annotation2137424
version: 1
top level: BBa_K510000
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K510000/annotation2137424/range2137424/1

https://synbiohub.org/public/igem/BBa_K510000/annotation2137424/range2137424/1
type: Range
persistent identity: range2137424
display id: range2137424
version: 1
top level: BBa_K510000
direction: direction/forward
start: 3224
end: 3318
orientation: inline

Gentamycin resistance
type: SequenceAnnotation
persistent identity: annotation2137421
display id: annotation2137421
version: 1
top level: BBa_K510000
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K510000/annotation2137421/range2137421/1

https://synbiohub.org/public/igem/BBa_K510000/annotation2137421/range2137421/1
type: Range
persistent identity: range2137421
display id: range2137421
version: 1
top level: BBa_K510000
direction: direction/reverse
start: 3393
end: 3926
orientation: inline

VF2 (G00100) primer binding site
type: SequenceAnnotation
persistent identity: annotation2137442
display id: annotation2137442
version: 1
top level: BBa_K510000
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K510000/annotation2137442/range2137442/1

https://synbiohub.org/public/igem/BBa_K510000/annotation2137442/range2137442/1
type: Range
persistent identity: range2137442
display id: range2137442
version: 1
top level: BBa_K510000
direction: direction/forward
start: 4249
end: 4268
orientation: inline

E. coli his operon terminator
type: SequenceAnnotation
persistent identity: annotation2137412
display id: annotation2137412
version: 1
top level: BBa_K510000
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K510000/annotation2137412/range2137412/1

https://synbiohub.org/public/igem/BBa_K510000/annotation2137412/range2137412/1
type: Range
persistent identity: range2137412
display id: range2137412
version: 1
top level: BBa_K510000
direction: direction/forward
start: 22
end: 93
orientation: inline

C
type: ComponentDefinition
persistent identity: BBa G0010
display id: BBa_G0010
version: 1
was generated by: iGEM to SBOL conversion
description: The nucleotide C
created: 2007-07-22T11:00:00Z
modified: 2015-08-31T04:07:28Z
mutable provenance: None.
owned by: james
owned by: myers
mutable description: This is a part consisting of only a single nucleotide ... C. It can act as a spacer between restriction sites and parts.
discontinued: false
dominant: false
experience: experience/None
group u list: _41_
m user id: 0
owner id: 126
owning group id: 162
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: None.
star: false
creator: Reshma Shetty
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_G0010_sequence/1

scar
type: ComponentDefinition
persistent identity: BBa K112999
display id: BBa_K112999
version: 1
was generated by: iGEM to SBOL conversion
description: BBb1 scar sequence
created: 2008-10-20T11:00:00Z
modified: 2015-05-08T01:09:20Z
owned by: james
owned by: myers
mutable description: This is the scar sequence of the BBb1 assembly scheme. For more information, refer to [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page].
discontinued: false
dominant: false
experience: experience/None
group u list: _224_
m user id: 0
owner id: 2998
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Bing Xia
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_K112999_sequence/1

AfeI
type: ComponentDefinition
persistent identity: BBa M31956
display id: BBa_M31956
version: 1
was generated by: iGEM to SBOL conversion
description: AfeI restriction enzyme site
created: 2007-02-27T12:00:00Z
modified: 2015-05-08T01:14:01Z
mutable provenance: known sequence
owned by: james
owned by: myers
mutable description: Restriction site
discontinued: false
dominant: false
experience: experience/None
group u list: _102_
m user id: 0
owner id: 1381
owning group id: 102
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: none
star: false
creator: Rebecca Adams
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_M31956_sequence/1

SapI
type: ComponentDefinition
persistent identity: BBa B0102
display id: BBa_B0102
version: 1
was generated by: iGEM to SBOL conversion
description: SapI restriction enzyme site (offset cutter)
created: 2007-03-05T12:00:00Z
modified: 2015-08-31T04:07:21Z
mutable provenance: NEB Catalog, SapI is part #R0569S.
owned by: james
owned by: myers
mutable description: SapI is an offsite cutter restriction site. The recognition sequence for this enzyme is the 8 bp sequence GCTCTTCN (on the 5' strand). Cleavage at this site results in a 3 bp, 3' overhang (right side, bottom strand) directly downstream of this sequence. This specific part has a T in the 8th position.
discontinued: false
dominant: false
experience: experience/None
group u list: _11_
m user id: 0
owner id: 571
owning group id: 10
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: This part was designed for use with Heather Keller's alternative assembly method for blunt cloning RBS sequences upstream of a coding sequence.
star: false
creator: Heather Keller
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: SapI recognition
sequence: https://synbiohub.org/public/igem/BBa_B0102_sequence/1

SapI recognition
type: SequenceAnnotation
persistent identity: annotation1920042
display id: annotation1920042
version: 1
top level: SapI
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_B0102/annotation1920042/range1920042/1

https://synbiohub.org/public/igem/BBa_B0102/annotation1920042/range1920042/1
type: Range
persistent identity: range1920042
display id: range1920042
version: 1
top level: SapI
direction: direction/forward
start: 1
end: 7
orientation: inline

NheI
type: ComponentDefinition
persistent identity: BBa B0045
display id: BBa_B0045
version: 1
was generated by: iGEM to SBOL conversion
description: NheI restriction enzyme site (XbaI, SpeI compatible overhang)
created: 2006-03-13T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: NheI recognition site. Digestion with NheI generates a compatible cohesive end to XbaI and SpeI.
discontinued: false
dominant: true
experience: experience/None
group u list: _41_6_
m user id: 0
owner id: 126
owning group id: 45
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Reshma Shetty
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: NheI site
sequence: https://synbiohub.org/public/igem/BBa_B0045_sequence/1

NheI site
type: SequenceAnnotation
persistent identity: annotation1822724
display id: annotation1822724
version: 1
top level: NheI
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_B0045/annotation1822724/range1822724/1

https://synbiohub.org/public/igem/BBa_B0045/annotation1822724/range1822724/1
type: Range
persistent identity: range1822724
display id: range1822724
version: 1
top level: NheI
direction: direction/forward
start: 1
end: 6
orientation: inline

BBa_K1973033
type: ComponentDefinition
persistent identity: BBa K1973033
display id: BBa_K1973033
version: 1
was generated by: iGEM to SBOL conversion
description: nahR-Psal (inducible promoter)
created: 2016-10-08T11:00:00Z
modified: 2016-10-09T11:17:26Z
mutable provenance: It was obtained by PCR from a plasmid containing this sequence. The PCR primers added the prefix and suffix needed.
owned by: james
owned by: myers
mutable description: nahR (Pseudomonas putida, see BBa_K1031610) is expressed under the constitutive Pr promoter. It encodes a regulatory protein that activates the Psal promoter (see BBa_J61051) in the presence of salicylate.
discontinued: false
dominant: false
group u list: _2440_
m user id: 30003
owner id: 30003
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: We had included the RBS BBa_B0034 in the PCR primers, as well as the scar it should have appeared in the cloning of the DNA piece.
star: false
creator: Laura Claret Fern??ndez
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: Psal
sequence annotation: nahR
sequence: https://synbiohub.org/public/igem/BBa_K1973033_sequence/1

Psal
type: SequenceAnnotation
persistent identity: annotation2489301
display id: annotation2489301
version: 1
top level: BBa_K1973033
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1973033/annotation2489301/range2489301/1

https://synbiohub.org/public/igem/BBa_K1973033/annotation2489301/range2489301/1
type: Range
persistent identity: range2489301
display id: range2489301
version: 1
top level: BBa_K1973033
direction: direction/forward
start: 901
end: 1039
orientation: inline

nahR
type: SequenceAnnotation
persistent identity: annotation2489302
display id: annotation2489302
version: 1
top level: BBa_K1973033
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1973033/annotation2489302/range2489302/1

https://synbiohub.org/public/igem/BBa_K1973033/annotation2489302/range2489302/1
type: Range
persistent identity: range2489302
display id: range2489302
version: 1
top level: BBa_K1973033
direction: direction/reverse
start: 1
end: 900
orientation: inline

MluI
type: ComponentDefinition
persistent identity: BBa M31988
display id: BBa_M31988
version: 1
was generated by: iGEM to SBOL conversion
description: MluI restriction enzyme site
created: 2007-02-28T12:00:00Z
modified: 2015-05-08T01:14:01Z
mutable provenance: none
owned by: james
owned by: myers
mutable description: MluI restriction enzyme site
discontinued: false
dominant: false
experience: experience/None
group u list: _102_
m user id: 0
owner id: 1373
owning group id: 102
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: none
star: false
creator: Tiffany Guo
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_M31988_sequence/1

5-fingers
type: ComponentDefinition
persistent identity: BBa J35005
display id: BBa_J35005
version: 1
was generated by: iGEM to SBOL conversion
description: 5-fingers arm
created: 2006-10-22T11:00:00Z
modified: 2015-08-31T04:08:47Z
mutable provenance: artificial sequence
owned by: james
owned by: myers
mutable description: DNA ORIGAMI BioBrick
discontinued: false
dominant: false
experience: experience/None
group u list: _64_
m user id: 0
owner id: 807
owning group id: 64
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: OligoAnalyzer 3.0 http://www.idtdna.com/analyzer/Applications/OligoAnalyzer/
star: false
creator: Andrey Kuznetsov
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_J35005_sequence/1

SpeI
type: ComponentDefinition
persistent identity: BBa M31992
display id: BBa_M31992
version: 1
was generated by: iGEM to SBOL conversion
description: SpeI restriction enzyme site
created: 2007-02-28T12:00:00Z
modified: 2015-05-08T01:14:01Z
mutable provenance: none
owned by: myers
owned by: james
mutable description: SpeI restriction enzyme site
discontinued: false
dominant: false
experience: experience/None
group u list: _102_
m user id: 0
owner id: 1373
owning group id: 102
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: none
star: false
creator: Tiffany Guo
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_M31992_sequence/1

AclI
type: ComponentDefinition
persistent identity: BBa I11072
display id: BBa_I11072
version: 1
was generated by: iGEM to SBOL conversion
description: AclI restriction enzyme site with extra nucleotides
created: 2004-09-06T11:00:00Z
modified: 2015-08-31T04:07:30Z
owned by: james
owned by: myers
mutable description: AclI restriction site with 3 flanking bp
discontinued: false
dominant: false
experience: experience/None
group u list: _2_
m user id: 0
owner id: 102
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: bu
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: AclI
sequence: https://synbiohub.org/public/igem/BBa_I11072_sequence/1

AclI
type: SequenceAnnotation
persistent identity: annotation1148109
display id: annotation1148109
version: 1
top level: AclI
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I11072/annotation1148109/range1148109/1

https://synbiohub.org/public/igem/BBa_I11072/annotation1148109/range1148109/1
type: Range
persistent identity: range1148109
display id: range1148109
version: 1
top level: AclI
direction: direction/forward
start: 4
end: 9
orientation: inline

BBa_J70034
type: ComponentDefinition
persistent identity: BBa J70034
display id: BBa_J70034
version: 1
was generated by: iGEM to SBOL conversion
description: A BioScaffold Part (Uses AarI) for Library Vector Preparation (see Part Design page)
created: 2008-12-27T12:00:00Z
modified: 2015-05-08T01:08:20Z
mutable provenance: Designed
owned by: james
owned by: myers
mutable description: When placed in a BioBrick assembly and cut with the restriction enzyme AarI, this part will direct the restriction enzyme to cut within the BioBrick scar still in testing, uses AarI (2) XmaI (1) See part design page or BBFRFC15, for details about use
discontinued: false
dominant: false
experience: experience/None
group u list: _41_
m user id: 0
owner id: 1201
owning group id: 41
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: TBA
star: false
creator: Julie Norville
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: XmaI
sequence annotation: AarI
sequence: https://synbiohub.org/public/igem/BBa_J70034_sequence/1

XmaI
type: SequenceAnnotation
persistent identity: annotation2000049
display id: annotation2000049
version: 1
top level: BBa_J70034
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_J70034/annotation2000049/range2000049/1

https://synbiohub.org/public/igem/BBa_J70034/annotation2000049/range2000049/1
type: Range
persistent identity: range2000049
display id: range2000049
version: 1
top level: BBa_J70034
direction: direction/forward
start: 20
end: 25
orientation: inline

AarI
type: SequenceAnnotation
persistent identity: annotation2000048
display id: annotation2000048
version: 1
top level: BBa_J70034
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J70034/annotation2000048/range2000048/1

https://synbiohub.org/public/igem/BBa_J70034/annotation2000048/range2000048/1
type: Range
persistent identity: range2000048
display id: range2000048
version: 1
top level: BBa_J70034
direction: direction/forward
start: 38
end: 44
orientation: inline

AarI
type: SequenceAnnotation
persistent identity: annotation2000047
display id: annotation2000047
version: 1
top level: BBa_J70034
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J70034/annotation2000047/range2000047/1

https://synbiohub.org/public/igem/BBa_J70034/annotation2000047/range2000047/1
type: Range
persistent identity: range2000047
display id: range2000047
version: 1
top level: BBa_J70034
direction: direction/forward
start: 1
end: 7
orientation: inline

BBa_K1614007
type: ComponentDefinition
persistent identity: BBa K1614007
display id: BBa_K1614007
version: 1
was generated by: iGEM to SBOL conversion
description: HRP-mimicking DNAzyme
creator: Frieda Anna Sorgenfrei
created: 2015-09-15T11:00:00Z
modified: 2015-09-16T06:57:25Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2031_
m user id: 12380
owner id: 24664
owning group id: 9
status: status/Planning
bookmark: true
mutable description: A DNAzyme with peroxidas acivity.
mutable provenance: Synthetic
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: HRP-mimicking DNAzyme
sequence: https://synbiohub.org/public/igem/BBa_K1614007_sequence/1

HRP-mimicking DNAzyme
type: SequenceAnnotation
persistent identity: annotation2458146
display id: annotation2458146
version: 1
top level: BBa_K1614007
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1614007/annotation2458146/range2458146/1

https://synbiohub.org/public/igem/BBa_K1614007/annotation2458146/range2458146/1
type: Range
persistent identity: range2458146
display id: range2458146
version: 1
direction: direction/forward
top level: BBa_K1614007
start: 1
end: 17
orientation: inline

Up
type: ComponentDefinition
persistent identity: BBa K1642005
display id: BBa_K1642005
version: 1
was generated by: iGEM to SBOL conversion
description: Upstream sequence from slr0168
created: 2015-08-14T11:00:00Z
modified: 2015-09-16T07:27:55Z
mutable provenance: Slr
owned by: james
owned by: myers
mutable description: Slr
discontinued: false
dominant: false
experience: experience/None
group u list: _2059_
m user id: 24405
owner id: 24405
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: Slr
star: false
creator: Can Yin
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_K1642005_sequence/1

BBa_I714032
type: ComponentDefinition
persistent identity: BBa I714032
display id: BBa_I714032
version: 1
was generated by: iGEM to SBOL conversion
description: OriT-S (Origin of Transfer for the pSC101-plasmid nic region)
created: 2007-10-20T11:00:00Z
modified: 2015-08-31T04:07:47Z
mutable provenance: pSC101 NCBI:NC_002056
owned by: james
owned by: myers
mutable description: OriT-S (Origin of Transfer for the pSC101-type conjugative plasmid) is the nic region, where the relaxosome nicks the plasmid and conjugative transfer by pSC101 plasmid machinery begins via rolling circle replication.
discontinued: false
dominant: false
experience: experience/Works
group u list: _142_
m user id: 0
owner id: 2248
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
star: false
creator: Mingzhi Qu
type: DnaRegion
role: partType/Conjugation
role: SO:0000724
sequence: https://synbiohub.org/public/igem/BBa_I714032_sequence/1

BccI
type: ComponentDefinition
persistent identity: BBa M31962
display id: BBa_M31962
version: 1
was generated by: iGEM to SBOL conversion
description: BccI restriction enzyme site
created: 2007-02-27T12:00:00Z
modified: 2015-05-08T01:14:01Z
mutable provenance: known sequence
owned by: james
owned by: myers
mutable description: restriction site
discontinued: false
dominant: false
experience: experience/None
group u list: _102_
m user id: 0
owner id: 1381
owning group id: 102
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: none
star: false
creator: Rebecca Adams
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_M31962_sequence/1

BBa_J36857
type: ComponentDefinition
persistent identity: BBa J36857
display id: BBa_J36857
version: 1
was generated by: iGEM to SBOL conversion
description: S35 linkaptamer
created: 2006-10-28T11:00:00Z
modified: 2015-08-31T04:08:47Z
mutable provenance: synthetic
owned by: james
owned by: myers
mutable description: S35 is the streptavidin-binding component of the A35 adaptamer which can physically link together the proteins thrombin and streptavidin. A35 contains a 30 bp double-stranded linking region between thrombin and streptavidin aptamer sequences.
discontinued: false
dominant: false
experience: experience/None
group u list: _65_
m user id: 0
owner id: 1245
owning group id: 65
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Linking region had to avoid disruption of aptamer secondary structure.
star: false
creator: Lewis Hahn
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_J36857_sequence/1

BmtI
type: ComponentDefinition
persistent identity: BBa I11075
display id: BBa_I11075
version: 1
was generated by: iGEM to SBOL conversion
description: BmtI restriction enzyme site with extra flanking nucleotides
created: 2004-09-06T11:00:00Z
modified: 2015-08-31T04:07:30Z
mutable provenance: NEB catalog
owned by: james
owned by: myers
mutable description: BmtI restriction site with three base pairs before site
discontinued: false
dominant: false
experience: experience/None
group u list: _2_
m user id: 0
owner id: 102
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: bu
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: BmtI
sequence: https://synbiohub.org/public/igem/BBa_I11075_sequence/1

BmtI
type: SequenceAnnotation
persistent identity: annotation1148641
display id: annotation1148641
version: 1
top level: BmtI
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I11075/annotation1148641/range1148641/1

https://synbiohub.org/public/igem/BBa_I11075/annotation1148641/range1148641/1
type: Range
persistent identity: range1148641
display id: range1148641
version: 1
top level: BmtI
direction: direction/forward
start: 4
end: 9
orientation: inline

scar
type: ComponentDefinition
persistent identity: BBa G0005
display id: BBa_G0005
version: 1
was generated by: iGEM to SBOL conversion
description: NheI/SpeI mixed site
created: 2007-02-26T12:00:00Z
modified: 2015-08-31T04:07:27Z
mutable provenance: NheI and SpeI
owned by: james
owned by: myers
mutable description: NheI/SpeI mixed site
discontinued: false
dominant: false
experience: experience/None
group u list: _41_
m user id: 0
owner id: 126
owning group id: 70
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: None.
star: false
creator: Reshma Shetty
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_G0005_sequence/1

BBa_J70040
type: ComponentDefinition
persistent identity: BBa J70040
display id: BBa_J70040
version: 1
was generated by: iGEM to SBOL conversion
description: A BioScaffold Part (Uses AarI, MabI) for Library Vector Preparation (see Part Design page)
created: 2009-01-22T12:00:00Z
modified: 2015-05-08T01:08:20Z
mutable provenance: Designed
owned by: james
owned by: myers
mutable description: When placed in a BioBrick assembly and cut with the restriction enzyme AarI, this part will direct the restriction enzyme to cut within the BioBrick scar still in testing, uses AarI (2), MabI(2), XmaI (1) * XmaI does not work in vector for BioScaffold part manipulations See part design page or BBFRFC15, for details about use
discontinued: false
dominant: false
experience: experience/None
group u list: _41_
m user id: 0
owner id: 1201
owning group id: 41
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: See BBa_J70034, this is an analogous part except it uses MabI
star: false
creator: Julie Norville
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: AarI
sequence annotation: MabI
sequence annotation: XmaI
sequence: https://synbiohub.org/public/igem/BBa_J70040_sequence/1

AarI
type: SequenceAnnotation
persistent identity: annotation2000126
display id: annotation2000126
version: 1
top level: BBa_J70040
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J70040/annotation2000126/range2000126/1

https://synbiohub.org/public/igem/BBa_J70040/annotation2000126/range2000126/1
type: Range
persistent identity: range2000126
display id: range2000126
version: 1
top level: BBa_J70040
direction: direction/forward
start: 1
end: 7
orientation: inline

AarI
type: SequenceAnnotation
persistent identity: annotation2000127
display id: annotation2000127
version: 1
top level: BBa_J70040
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J70040/annotation2000127/range2000127/1

https://synbiohub.org/public/igem/BBa_J70040/annotation2000127/range2000127/1
type: Range
persistent identity: range2000127
display id: range2000127
version: 1
top level: BBa_J70040
direction: direction/forward
start: 38
end: 44
orientation: inline

MabI
type: SequenceAnnotation
persistent identity: annotation2000129
display id: annotation2000129
version: 1
top level: BBa_J70040
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_J70040/annotation2000129/range2000129/1

https://synbiohub.org/public/igem/BBa_J70040/annotation2000129/range2000129/1
type: Range
persistent identity: range2000129
display id: range2000129
version: 1
top level: BBa_J70040
direction: direction/forward
start: 11
end: 17
orientation: inline

XmaI
type: SequenceAnnotation
persistent identity: annotation2000128
display id: annotation2000128
version: 1
top level: BBa_J70040
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_J70040/annotation2000128/range2000128/1

https://synbiohub.org/public/igem/BBa_J70040/annotation2000128/range2000128/1
type: Range
persistent identity: range2000128
display id: range2000128
version: 1
top level: BBa_J70040
direction: direction/forward
start: 20
end: 25
orientation: inline

MabI
type: SequenceAnnotation
persistent identity: annotation2000130
display id: annotation2000130
version: 1
top level: BBa_J70040
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_J70040/annotation2000130/range2000130/1

https://synbiohub.org/public/igem/BBa_J70040/annotation2000130/range2000130/1
type: Range
persistent identity: range2000130
display id: range2000130
version: 1
top level: BBa_J70040
direction: direction/forward
start: 29
end: 34
orientation: inline

K592202
type: ComponentDefinition
persistent identity: BBa K592202
display id: BBa_K592202
version: 1
was generated by: iGEM to SBOL conversion
description: Low to medium copy Lambda Red recombineering compatible plasmid
created: 2011-09-20T11:00:00Z
modified: 2015-05-08T01:12:49Z
mutable provenance: The P22 late terminator come from the genome of the Salmonella phage P22 (GenBank: AF217253.1, 19662..19702), and the kanamycin resistance marker flanked by FRT sites comes from the pKD4 plasmid (Datsenko and Wanner, 2000).
owned by: james
owned by: myers
mutable description: BBa_K592202 is a BioBrick friendly vector with low to medium copy p15A replication origin (BBa_I50032) and a kanamycin antibiotic resistance marker, usable for Lambda Red recombineering in E coli. The E coli His operon terminator BBa_B0053 has been replaced with the late terminator of the Salmonella phage P22, and the kanamycin resistance marker is flanked by FRT sites so that the resistance marker can be removed after chromosomal integration using the FLP recombinase.
discontinued: false
dominant: false
experience: experience/Works
group u list: _763_
m user id: 0
owner id: 10133
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: To make the plasmid usable for Lambda Red recombineering, all sequences with homologies to the E coli chromosome had to be removed. To accomplish this, the E coli His operon terminator BBa_B0053 has been replaced with the late terminator of the Salmonella phage P22. To be able to remove the antibiotic resistance marker after chromosomal integration, the kanamycin marker from the pKD4 plasmid (Datsenko and Wanner, 2000) was used. The resistance marker can be removed after chromosomal integration using the FLP recombinase, leaving only the plasmid insert and a scar behind.
star: false
creator: Antonio Ascue Avalos
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: Terminator BBa_B0062
sequence annotation: Translational stop BBa_B0042
sequence annotation: P22 late terminator
sequence annotation: T3 terminator BBa_B0055
sequence annotation: L-red_F_primer
sequence annotation: FRT site
sequence annotation: p15A ori BBa_I50032
sequence annotation: Terminator BBa_B0054
sequence annotation: Kanamycin resistance marker
sequence annotation: VR site BBa_G00102
sequence annotation: VF2 site BBa_G00100
sequence annotation: L-red_R_primer
sequence: https://synbiohub.org/public/igem/BBa_K592202_sequence/1

Terminator BBa_B0062
type: SequenceAnnotation
persistent identity: annotation2137822
display id: annotation2137822
version: 1
top level: K592202
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K592202/annotation2137822/range2137822/1

https://synbiohub.org/public/igem/BBa_K592202/annotation2137822/range2137822/1
type: Range
persistent identity: range2137822
display id: range2137822
version: 1
top level: K592202
direction: direction/forward
start: 136
end: 176
orientation: inline

Translational stop BBa_B0042
type: SequenceAnnotation
persistent identity: annotation2137804
display id: annotation2137804
version: 1
top level: K592202
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K592202/annotation2137804/range2137804/1

https://synbiohub.org/public/igem/BBa_K592202/annotation2137804/range2137804/1
type: Range
persistent identity: range2137804
display id: range2137804
version: 1
top level: K592202
direction: direction/forward
start: 35
end: 46
orientation: inline

P22 late terminator
type: SequenceAnnotation
persistent identity: annotation2137828
display id: annotation2137828
version: 1
top level: K592202
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K592202/annotation2137828/range2137828/1

https://synbiohub.org/public/igem/BBa_K592202/annotation2137828/range2137828/1
type: Range
persistent identity: range2137828
display id: range2137828
version: 1
top level: K592202
direction: direction/forward
start: 3245
end: 3285
orientation: inline

T3 terminator BBa_B0055
type: SequenceAnnotation
persistent identity: annotation2137830
display id: annotation2137830
version: 1
top level: K592202
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K592202/annotation2137830/range2137830/1

https://synbiohub.org/public/igem/BBa_K592202/annotation2137830/range2137830/1
type: Range
persistent identity: range2137830
display id: range2137830
version: 1
top level: K592202
direction: direction/forward
start: 3306
end: 3383
orientation: inline

L-red_F_primer
type: SequenceAnnotation
persistent identity: annotation2137824
display id: annotation2137824
version: 1
top level: K592202
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K592202/annotation2137824/range2137824/1

https://synbiohub.org/public/igem/BBa_K592202/annotation2137824/range2137824/1
type: Range
persistent identity: range2137824
display id: range2137824
version: 1
top level: K592202
direction: direction/forward
start: 1726
end: 1745
orientation: inline

FRT site
type: SequenceAnnotation
persistent identity: annotation2137825
display id: annotation2137825
version: 1
top level: K592202
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K592202/annotation2137825/range2137825/1

https://synbiohub.org/public/igem/BBa_K592202/annotation2137825/range2137825/1
type: Range
persistent identity: range2137825
display id: range2137825
version: 1
top level: K592202
direction: direction/forward
start: 1781
end: 1826
orientation: inline

Translational stop BBa_B0042
type: SequenceAnnotation
persistent identity: annotation2137846
display id: annotation2137846
version: 1
top level: K592202
role: feature/stop
role: SO:0000319
location: https://synbiohub.org/public/igem/BBa_K592202/annotation2137846/range2137846/1

https://synbiohub.org/public/igem/BBa_K592202/annotation2137846/range2137846/1
type: Range
persistent identity: range2137846
display id: range2137846
version: 1
top level: K592202
direction: direction/forward
start: 3384
end: 3395
orientation: inline

p15A ori BBa_I50032
type: SequenceAnnotation
persistent identity: annotation2137823
display id: annotation2137823
version: 1
top level: K592202
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K592202/annotation2137823/range2137823/1

https://synbiohub.org/public/igem/BBa_K592202/annotation2137823/range2137823/1
type: Range
persistent identity: range2137823
display id: range2137823
version: 1
top level: K592202
direction: direction/forward
start: 184
end: 1748
orientation: inline

Terminator BBa_B0054
type: SequenceAnnotation
persistent identity: annotation2137820
display id: annotation2137820
version: 1
top level: K592202
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K592202/annotation2137820/range2137820/1

https://synbiohub.org/public/igem/BBa_K592202/annotation2137820/range2137820/1
type: Range
persistent identity: range2137820
display id: range2137820
version: 1
top level: K592202
direction: direction/forward
start: 47
end: 115
orientation: inline

Kanamycin resistance marker
type: SequenceAnnotation
persistent identity: annotation2137826
display id: annotation2137826
version: 1
top level: K592202
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K592202/annotation2137826/range2137826/1

https://synbiohub.org/public/igem/BBa_K592202/annotation2137826/range2137826/1
type: Range
persistent identity: range2137826
display id: range2137826
version: 1
top level: K592202
direction: direction/reverse
start: 2017
end: 2811
orientation: inline

VR site BBa_G00102
type: SequenceAnnotation
persistent identity: annotation2137821
display id: annotation2137821
version: 1
top level: K592202
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K592202/annotation2137821/range2137821/1

https://synbiohub.org/public/igem/BBa_K592202/annotation2137821/range2137821/1
type: Range
persistent identity: range2137821
display id: range2137821
version: 1
top level: K592202
direction: direction/reverse
start: 116
end: 135
orientation: inline

FRT site
type: SequenceAnnotation
persistent identity: annotation2137827
display id: annotation2137827
version: 1
top level: K592202
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K592202/annotation2137827/range2137827/1

https://synbiohub.org/public/igem/BBa_K592202/annotation2137827/range2137827/1
type: Range
persistent identity: range2137827
display id: range2137827
version: 1
top level: K592202
direction: direction/forward
start: 3172
end: 3219
orientation: inline

VF2 site BBa_G00100
type: SequenceAnnotation
persistent identity: annotation2137829
display id: annotation2137829
version: 1
top level: K592202
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K592202/annotation2137829/range2137829/1

https://synbiohub.org/public/igem/BBa_K592202/annotation2137829/range2137829/1
type: Range
persistent identity: range2137829
display id: range2137829
version: 1
top level: K592202
direction: direction/forward
start: 3286
end: 3305
orientation: inline

L-red_R_primer
type: SequenceAnnotation
persistent identity: annotation2137792
display id: annotation2137792
version: 1
top level: K592202
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K592202/annotation2137792/range2137792/1

https://synbiohub.org/public/igem/BBa_K592202/annotation2137792/range2137792/1
type: Range
persistent identity: range2137792
display id: range2137792
version: 1
top level: K592202
direction: direction/reverse
start: 20
end: 41
orientation: inline

MluI
type: ComponentDefinition
persistent identity: BBa I11079
display id: BBa_I11079
version: 1
was generated by: iGEM to SBOL conversion
description: MluI restriction enzyme site with flanking nucleotides
created: 2004-09-06T11:00:00Z
modified: 2015-08-31T04:07:30Z
mutable provenance: NEB catalog
owned by: james
owned by: myers
mutable description: MluI restriction site
discontinued: false
dominant: false
experience: experience/None
group u list: _2_
m user id: 0
owner id: 102
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: bu
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_I11079_sequence/1

BBa_I13451
type: ComponentDefinition
persistent identity: BBa I13451
display id: BBa_I13451
version: 1
was generated by: iGEM to SBOL conversion
description: Removes left Biobrick end
created: 2005-05-24T11:00:00Z
modified: 2015-08-31T04:07:34Z
owned by: james
owned by: myers
mutable description: Contains an MfeI restriction site. Cut this part with MfeI+Spe and the other part with Eco+Xba. Ligation will form two mixed sites, neither of which can be recut. This effectively fixes the left side of the Biobrick part
discontinued: true
dominant: false
experience: experience/None
group u list: _11_
m user id: 0
owner id: 253
owning group id: 6
sample status: Discontinued
status: status/Deleted
bookmark: false
star: false
creator: jkm
type: DnaRegion
role: partType/DNA
role: SO:0000110

oriT/LacZ
type: ComponentDefinition
persistent identity: BBa K125360
display id: BBa_K125360
version: 1
was generated by: iGEM to SBOL conversion
description: oriT with lac promoter and lacZ
created: 2008-10-27T12:00:00Z
modified: 2015-05-08T01:09:45Z
mutable provenance: The oriT sequence was taken from the self-transmissible plasmid RP4. LacZ is available as a part in the registry.
owned by: james
owned by: myers
mutable description: This part was constructed to compare the oriTr, which is currently available in the registry to oriT which was recently designed to perform the same function. The oriTr was added to LacZ part so that conjugation could be monitored by blue/white selection.
discontinued: false
dominant: false
experience: experience/None
group u list: _179_
m user id: 0
owner id: 3138
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: The oriT was synthesized and J33207 was extracted from the registry.
star: false
creator: Margaret Ruzicka
type: DnaRegion
role: partType/Conjugation
role: SO:0000724
component: BBa_K125320
component: BBa_J33207
sequence annotation: BBa_K125320
sequence annotation: BBa_J33207
sequence: https://synbiohub.org/public/igem/BBa_K125360_sequence/1

BBa_K125320
type: Component
persistent identity: component1992572
display id: component1992572
version: 1
top level: oriT/LacZ
access: public
definition: OriT-RP4

BBa_J33207
type: Component
persistent identity: component1992571
display id: component1992571
version: 1
top level: oriT/LacZ
access: public
definition: BBa_J33207

BBa_K125320
type: SequenceAnnotation
persistent identity: annotation1992572
display id: annotation1992572
version: 1
top level: oriT/LacZ
location: https://synbiohub.org/public/igem/BBa_K125360/annotation1992572/range1992572/1
component: BBa_K125320

https://synbiohub.org/public/igem/BBa_K125360/annotation1992572/range1992572/1
type: Range
persistent identity: range1992572
display id: range1992572
version: 1
top level: oriT/LacZ
direction: direction/reverse
start: 609
end: 707
orientation: inline

BBa_J33207
type: SequenceAnnotation
persistent identity: annotation1992571
display id: annotation1992571
version: 1
top level: oriT/LacZ
location: https://synbiohub.org/public/igem/BBa_K125360/annotation1992571/range1992571/1
component: BBa_J33207

https://synbiohub.org/public/igem/BBa_K125360/annotation1992571/range1992571/1
type: Range
persistent identity: range1992571
display id: range1992571
version: 1
top level: oriT/LacZ
direction: direction/reverse
start: 1
end: 600
orientation: inline

O2
type: ComponentDefinition
persistent identity: BBa K2054002
display id: BBa_K2054002
version: 1
was generated by: iGEM to SBOL conversion
description: Oligo 2 of DNA Tetrahedral Nanostructure
creator: LAI HEI WAI
created: 2016-10-13T11:00:00Z
modified: 2016-10-27T11:56:17Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2522_
m user id: 30316
owner id: 30316
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This will be used to build our desired nanostructure. This plasmid encodes the second ssDNA that will make the tetrahedral nanostructure, together with oligo 1, 3, 4 and 5.
mutable notes: The assembly of the structure.
mutable provenance: Elbaz, J., Yin, P., & Voigt, C. A. (2016). Genetic encoding of DNA nanostructures and their self-assembly in living bacteria. Nature communications, 7.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_K2054002_sequence/1

BBa_J70030
type: ComponentDefinition
persistent identity: BBa J70030
display id: BBa_J70030
version: 1
was generated by: iGEM to SBOL conversion
description: A BioScaffold Part (Uses PpiI), Protein Tail Remover see Part Design Page
created: 2008-12-07T12:00:00Z
modified: 2015-05-08T01:08:20Z
mutable provenance: this part is designed
owned by: james
owned by: myers
mutable description: When placed in a BioBrick assembly and cut with the restriction enzyme PpiI, this part will direct the restriction enzymes to cut outside the BioBrick scar still in testing, uses restriction enzyme sites PpiI (2) PacI (2) MabI (2), will place in pSB1AK3 vector
discontinued: false
dominant: false
experience: experience/None
group u list: _41_
m user id: 0
owner id: 1201
owning group id: 41
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Can be used to removed protein tails for protein fusions, see BBF RFC 15 XXXXX^ XXX TACTAGAG BioScaffold Part BBa_J70030 TACTAGAT XXXXX^ ^XXXXX XXX ATGATCAC ATGATCTA^XXXXX XXXXX^ TAA TACTAGAG BioScaffold Part BBa_J70030 TACTAGAT XXXXX^ ^XXXXX ATT ATGATCAC ATGATCTA^XXXXX
star: false
creator: Julie Norville
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: PpiI 2
sequence annotation: PacI 2
sequence annotation: PacI3
sequence annotation: MabI 2
sequence annotation: PpiI 1
sequence annotation: PacI 1
sequence annotation: MabI 1
sequence: https://synbiohub.org/public/igem/BBa_J70030_sequence/1

PpiI 2
type: SequenceAnnotation
persistent identity: annotation2000014
display id: annotation2000014
version: 1
top level: BBa_J70030
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J70030/annotation2000014/range2000014/1

https://synbiohub.org/public/igem/BBa_J70030/annotation2000014/range2000014/1
type: Range
persistent identity: range2000014
display id: range2000014
version: 1
top level: BBa_J70030
direction: direction/forward
start: 37
end: 40
orientation: inline

PacI 2
type: SequenceAnnotation
persistent identity: annotation2000012
display id: annotation2000012
version: 1
top level: BBa_J70030
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_J70030/annotation2000012/range2000012/1

https://synbiohub.org/public/igem/BBa_J70030/annotation2000012/range2000012/1
type: Range
persistent identity: range2000012
display id: range2000012
version: 1
top level: BBa_J70030
direction: direction/forward
start: 22
end: 29
orientation: inline

PacI3
type: SequenceAnnotation
persistent identity: annotation2000011
display id: annotation2000011
version: 1
top level: BBa_J70030
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_J70030/annotation2000011/range2000011/1

https://synbiohub.org/public/igem/BBa_J70030/annotation2000011/range2000011/1
type: Range
persistent identity: range2000011
display id: range2000011
version: 1
top level: BBa_J70030
direction: direction/forward
start: 18
end: 24
orientation: inline

MabI 2
type: SequenceAnnotation
persistent identity: annotation2000013
display id: annotation2000013
version: 1
top level: BBa_J70030
role: feature/dna
role: SO:0000110
location: https://synbiohub.org/public/igem/BBa_J70030/annotation2000013/range2000013/1

https://synbiohub.org/public/igem/BBa_J70030/annotation2000013/range2000013/1
type: Range
persistent identity: range2000013
display id: range2000013
version: 1
top level: BBa_J70030
direction: direction/forward
start: 30
end: 36
orientation: inline

PpiI 1
type: SequenceAnnotation
persistent identity: annotation2000008
display id: annotation2000008
version: 1
top level: BBa_J70030
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J70030/annotation2000008/range2000008/1

https://synbiohub.org/public/igem/BBa_J70030/annotation2000008/range2000008/1
type: Range
persistent identity: range2000008
display id: range2000008
version: 1
top level: BBa_J70030
direction: direction/forward
start: 4
end: 7
orientation: inline

PpiI 2
type: SequenceAnnotation
persistent identity: annotation2000015
display id: annotation2000015
version: 1
top level: BBa_J70030
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J70030/annotation2000015/range2000015/1

https://synbiohub.org/public/igem/BBa_J70030/annotation2000015/range2000015/1
type: Range
persistent identity: range2000015
display id: range2000015
version: 1
top level: BBa_J70030
direction: direction/forward
start: 46
end: 48
orientation: inline

PacI 1
type: SequenceAnnotation
persistent identity: annotation2000010
display id: annotation2000010
version: 1
top level: BBa_J70030
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_J70030/annotation2000010/range2000010/1

https://synbiohub.org/public/igem/BBa_J70030/annotation2000010/range2000010/1
type: Range
persistent identity: range2000010
display id: range2000010
version: 1
top level: BBa_J70030
direction: direction/forward
start: 14
end: 20
orientation: inline

MabI 1
type: SequenceAnnotation
persistent identity: annotation2000009
display id: annotation2000009
version: 1
top level: BBa_J70030
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_J70030/annotation2000009/range2000009/1

https://synbiohub.org/public/igem/BBa_J70030/annotation2000009/range2000009/1
type: Range
persistent identity: range2000009
display id: range2000009
version: 1
top level: BBa_J70030
direction: direction/forward
start: 8
end: 14
orientation: inline

NsiI
type: ComponentDefinition
persistent identity: BBa B0046
display id: BBa_B0046
version: 1
was generated by: iGEM to SBOL conversion
description: NsiI restriction enzyme site (PstI compatible overhang)
created: 2006-03-13T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: NsiI recognition site. Digestion with NsiI generates a compatible cohesive end to PstI.
discontinued: false
dominant: false
experience: experience/None
group u list: _41_6_
m user id: 0
owner id: 126
owning group id: 45
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Reshma Shetty
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: NsiI site
sequence: https://synbiohub.org/public/igem/BBa_B0046_sequence/1

NsiI site
type: SequenceAnnotation
persistent identity: annotation1822730
display id: annotation1822730
version: 1
top level: NsiI
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_B0046/annotation1822730/range1822730/1

https://synbiohub.org/public/igem/BBa_B0046/annotation1822730/range1822730/1
type: Range
persistent identity: range1822730
display id: range1822730
version: 1
top level: NsiI
direction: direction/forward
start: 1
end: 6
orientation: inline

B-Z DNA sw
type: ComponentDefinition
persistent identity: BBa J35120
display id: BBa_J35120
version: 1
was generated by: iGEM to SBOL conversion
description: B-Z DNA switcher
created: 2006-10-23T11:00:00Z
modified: 2015-08-31T04:08:47Z
mutable provenance: artificial sequence
owned by: james
owned by: myers
mutable description: This DNA will be used as a part of DNA-origami device. The mechanical movement will be performed by B???Z DNA transition in the presence/absence of Co(NH3)6Cl3.
discontinued: false
dominant: false
experience: experience/Works
group u list: _
m user id: 0
owner id: 807
owning group id: 64
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Mao C, Sun W, Shen Z, Seeman NC. A nanomechanical device based on the B-Z transition of DNA. Nature. 1999 Jan 14;397(6715):144-6.
star: false
creator: Andrey Kuznetsov
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_J35120_sequence/1

BglII
type: ComponentDefinition
persistent identity: BBa M31985
display id: BBa_M31985
version: 1
was generated by: iGEM to SBOL conversion
description: BglII restriction enzyme site
created: 2007-02-28T12:00:00Z
modified: 2015-05-08T01:14:01Z
mutable provenance: none
owned by: james
owned by: myers
mutable description: BglII restriction enzyme site
discontinued: false
dominant: false
experience: experience/None
group u list: _102_
m user id: 0
owner id: 1373
owning group id: 102
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: none
star: false
creator: Tiffany Guo
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_M31985_sequence/1

Prefix
type: ComponentDefinition
persistent identity: BBa G00000
display id: BBa_G00000
version: 1
was generated by: iGEM to SBOL conversion
description: BioBrick cloning site prefix
created: 2006-03-13T12:00:00Z
modified: 2015-08-31T04:07:27Z
owned by: james
owned by: myers
mutable description: This part is the standard BioBricks prefix.
discontinued: false
dominant: true
experience: experience/Works
group u list: _41_6_
m user id: 0
owner id: 126
owning group id: 45
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Reshma Shetty
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: XbaI site
sequence annotation: NotI site
sequence annotation: Prefix
sequence annotation: EcoRI site
sequence: https://synbiohub.org/public/igem/BBa_G00000_sequence/1

XbaI site
type: SequenceAnnotation
persistent identity: annotation1934224
display id: annotation1934224
version: 1
top level: Prefix
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_G00000/annotation1934224/range1934224/1

https://synbiohub.org/public/igem/BBa_G00000/annotation1934224/range1934224/1
type: Range
persistent identity: range1934224
display id: range1934224
version: 1
top level: Prefix
direction: direction/forward
start: 16
end: 21
orientation: inline

NotI site
type: SequenceAnnotation
persistent identity: annotation1961228
display id: annotation1961228
version: 1
top level: Prefix
role: feature/dna
role: SO:0000110
location: https://synbiohub.org/public/igem/BBa_G00000/annotation1961228/range1961228/1

https://synbiohub.org/public/igem/BBa_G00000/annotation1961228/range1961228/1
type: Range
persistent identity: range1961228
display id: range1961228
version: 1
top level: Prefix
direction: direction/forward
start: 7
end: 14
orientation: inline

Prefix
type: SequenceAnnotation
persistent identity: annotation1934225
display id: annotation1934225
version: 1
top level: Prefix
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_G00000/annotation1934225/range1934225/1

https://synbiohub.org/public/igem/BBa_G00000/annotation1934225/range1934225/1
type: Range
persistent identity: range1934225
display id: range1934225
version: 1
top level: Prefix
direction: direction/forward
start: 1
end: 22
orientation: inline

EcoRI site
type: SequenceAnnotation
persistent identity: annotation1934223
display id: annotation1934223
version: 1
top level: Prefix
role: feature/dna
role: SO:0000110
location: https://synbiohub.org/public/igem/BBa_G00000/annotation1934223/range1934223/1

https://synbiohub.org/public/igem/BBa_G00000/annotation1934223/range1934223/1
type: Range
persistent identity: range1934223
display id: range1934223
version: 1
top level: Prefix
direction: direction/forward
start: 1
end: 6
orientation: inline

AseI
type: ComponentDefinition
persistent identity: BBa M31958
display id: BBa_M31958
version: 1
was generated by: iGEM to SBOL conversion
description: AseI restriction enzyme site
created: 2007-02-27T12:00:00Z
modified: 2015-05-08T01:14:01Z
mutable provenance: known sequence
owned by: james
owned by: myers
mutable description: Restriction site
discontinued: false
dominant: false
experience: experience/None
group u list: _102_
m user id: 0
owner id: 1381
owning group id: 102
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: none
star: false
creator: Rebecca Adams
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_M31958_sequence/1

BBa_J36858
type: ComponentDefinition
persistent identity: BBa J36858
display id: BBa_J36858
version: 1
was generated by: iGEM to SBOL conversion
description: S20 linkaptamer
created: 2006-10-28T11:00:00Z
modified: 2015-08-31T04:08:47Z
mutable provenance: synthetic
owned by: james
owned by: myers
mutable description: S20 is the streptavidin-binding component of the A20 adaptamer which can physically link together the proteins thrombin and streptavidin. A20 contains a 15 bp double-stranded linking region between thrombin and streptavidin aptamer sequences.
discontinued: false
dominant: false
experience: experience/None
group u list: _65_
m user id: 0
owner id: 1245
owning group id: 65
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable notes: Linking region had to avoid disruption of aptamer secondary structure.
star: false
creator: Lewis Hahn
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_J36858_sequence/1

BBa_K1973006
type: ComponentDefinition
persistent identity: BBa K1973006
display id: BBa_K1973006
version: 1
was generated by: iGEM to SBOL conversion
description: miniTn7BB-Gm-nahR-Psal-lapG
created: 2016-10-12T11:00:00Z
modified: 2016-10-13T09:24:18Z
mutable provenance: Basic parts were cloned into the miniTn7BB-Gm Backbone
owned by: james
owned by: myers
mutable description: nahR (Pseudomonas putida, see BBa_K1031610) is expressed under the constitutive Pr promoter. It encodes a regulatory protein that activates the Psal promoter (see BBa_J61051) in the presence of salicylate. lapG (see BBa_K1973000) is expressed from the Psal promoter in the presence of this molecule. The miniTn7 tool allows the stable integration of this module in the chromosome of a wide range of bacteria (see http://parts.igem.org/Genome_Integration).
discontinued: false
dominant: false
group u list: _2440_
m user id: 29968
owner id: 29968
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: Designs are described in basic parts pages
star: false
creator: Gabriel Ruiz Romero
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K510000
component: BBa_K1973000
component: BBa_K1973033
sequence annotation: BBa_K1973033
sequence annotation: BBa_K510000
sequence annotation: BBa_K1973000
sequence: https://synbiohub.org/public/igem/BBa_K1973006_sequence/1

BBa_K510000
type: Component
persistent identity: component2498468
display id: component2498468
version: 1
top level: BBa_K1973006
access: public
definition: BBa_K510000

BBa_K1973000
type: Component
persistent identity: component2498475
display id: component2498475
version: 1
top level: BBa_K1973006
access: public
definition: BBa_K1973000

BBa_K1973033
type: Component
persistent identity: component2498471
display id: component2498471
version: 1
top level: BBa_K1973006
access: public
definition: BBa_K1973033

BBa_K1973033
type: SequenceAnnotation
persistent identity: annotation2498471
display id: annotation2498471
version: 1
top level: BBa_K1973006
location: https://synbiohub.org/public/igem/BBa_K1973006/annotation2498471/range2498471/1
component: BBa_K1973033

https://synbiohub.org/public/igem/BBa_K1973006/annotation2498471/range2498471/1
type: Range
persistent identity: range2498471
display id: range2498471
version: 1
top level: BBa_K1973006
direction: direction/reverse
start: 4397
end: 5435
orientation: inline

BBa_K510000
type: SequenceAnnotation
persistent identity: annotation2498468
display id: annotation2498468
version: 1
top level: BBa_K1973006
location: https://synbiohub.org/public/igem/BBa_K1973006/annotation2498468/range2498468/1
component: BBa_K510000

https://synbiohub.org/public/igem/BBa_K1973006/annotation2498468/range2498468/1
type: Range
persistent identity: range2498468
display id: range2498468
version: 1
top level: BBa_K1973006
direction: direction/reverse
start: 1
end: 4388
orientation: inline

BBa_K1973000
type: SequenceAnnotation
persistent identity: annotation2498475
display id: annotation2498475
version: 1
top level: BBa_K1973006
location: https://synbiohub.org/public/igem/BBa_K1973006/annotation2498475/range2498475/1
component: BBa_K1973000

https://synbiohub.org/public/igem/BBa_K1973006/annotation2498475/range2498475/1
type: Range
persistent identity: range2498475
display id: range2498475
version: 1
top level: BBa_K1973006
direction: direction/reverse
start: 5444
end: 6106
orientation: inline

BBa_K175002
type: ComponentDefinition
persistent identity: BBa K175002
display id: BBa_K175002
version: 1
was generated by: iGEM to SBOL conversion
description: I-PpoI homing endonuclease site
created: 2009-07-06T11:00:00Z
modified: 2015-05-08T01:10:59Z
mutable provenance: Muscarella,D.E., Ellison,E.L., Ruoff,B.M. and Vogt,V.M. (1990) Characterization of I-Ppo, an intron-encoded endonuclease that mediates homing of a group I intron in the ribosomal DNA of Physarum polycephalum. Mol. Cell. Biol., 10, 3386???3396.
owned by: james
owned by: myers
mutable description: Restriction site for the homing endonuclease I-PpoI.
discontinued: false
dominant: false
group u list: _280_
m user id: 0
owner id: 4286
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: None
star: false
creator: Calin Plesa
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_K175002_sequence/1

spacer
type: ComponentDefinition
persistent identity: BBa B0040
display id: BBa_B0040
version: 1
was generated by: iGEM to SBOL conversion
description: Spacer.1 (generic)
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Randomly generated and optimized for several parameters (see Design notes).
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Generic spacer for ensuring a 70 bp distance between the end of the suffix of the BioBrick part containing the double terminator and the prefix of the BioBrick part containing the promoter of the new gene. Please, use the AlignX function of Vector NT to check for homology with the components in your plasmid before using this spacer.
discontinued: false
dominant: false
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: The size of the spacer was choosed to meet the minimum length of a sequence that can be queried using the BLAST search engine. However, subsequences of it can be used to design shorter spacers. The sequence was selected from many more sequences randomly generated using the Random DNA Generator engine; the GC% parameter used as input was 50%. The sequences were selected based on the following constraints listed in their order of importance: the absence of any putative promoter regions, a low degree of homology with the Elowitz plasmid (whose components are widely used in our designs), no homology with other E.coli sequences as shown by BLASTN search results and the presence of a number of TAA stop codons. The second constraint was the most stringent leading to the elimination of most sequences. DE made the following changes to the original sequence in order to add stop codons in the -3 frame and more in the +2 frame (note, not all of these stop codons are UAA. Thus, if used in an organism that inserts an amino acid @ UGA or UAG the obvious will occur): T->A @ 85 T->A @ 42 C->T @ 79 A->T @ 64 A->T @ 31 T->A @ 34 C->A @ 37 Also, note that the above changes further reduce (the already very weak) homology to current NCBI-stored sequences. In the process of selecting the best sequence it appeared that a good alternative sequence for a spacer would be: AGGTTCTGATATGTAACTGTGCCCAATGTCGTTAGTGACGCATACCTCTTAAGAGGCCACTGTCCTAACA. The sequence contains no putative promoters and shows moderate homology with the 5' end of the Ampicillin resistance gene. However a strong promoter sequence starts 12 bp downstream of this sequence, and therefore the sequence presented above was preferred. The sequence is compatible (does not show significant homology) with the components in the Elowitz repressilator plasmid.
star: true
creator: Vinay S. Mahajan, Brian Chow, Peter Carr
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: BBa_B0040
sequence annotation: Spacer-1
sequence: https://synbiohub.org/public/igem/BBa_B0040_sequence/1

BBa_B0040
type: SequenceAnnotation
persistent identity: annotation7030
display id: annotation7030
version: 1
top level: spacer
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_B0040/annotation7030/range7030/1

https://synbiohub.org/public/igem/BBa_B0040/annotation7030/range7030/1
type: Range
persistent identity: range7030
display id: range7030
version: 1
top level: spacer
direction: direction/forward
start: 1
end: 70
orientation: inline

Spacer-1
type: SequenceAnnotation
persistent identity: annotation1721
display id: annotation1721
version: 1
top level: spacer
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_B0040/annotation1721/range1721/1

https://synbiohub.org/public/igem/BBa_B0040/annotation1721/range1721/1
type: Range
persistent identity: range1721
display id: range1721
version: 1
top level: spacer
direction: direction/forward
start: 1
end: 70
orientation: inline

oriTr/LacZ
type: ComponentDefinition
persistent identity: BBa K125350
display id: BBa_K125350
version: 1
was generated by: iGEM to SBOL conversion
description: oriTr with lac promoter and lacZ
created: 2008-10-27T12:00:00Z
modified: 2015-05-08T01:09:45Z
mutable provenance: This part was built from oriTr and LacZ.
owned by: james
owned by: myers
mutable description: This part was constructed to compare the oriTr, which is currently available in the registry to oriT which was recently designed to perform the same function. The oriTr was added to LacZ part so that conjugation could be monitored by blue/white selection.
discontinued: false
dominant: false
experience: experience/None
group u list: _179_
m user id: 0
owner id: 3138
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: This part will be tested by blue/white screening.
star: false
creator: Margaret Ruzicka
type: DnaRegion
role: partType/Conjugation
role: SO:0000724
component: BBa_J01003
component: BBa_J33207
sequence annotation: BBa_J33207
sequence annotation: BBa_J01003
sequence: https://synbiohub.org/public/igem/BBa_K125350_sequence/1

BBa_J01003
type: Component
persistent identity: component1992563
display id: component1992563
version: 1
top level: oriTr/LacZ
access: public
definition: OriTR

BBa_J33207
type: Component
persistent identity: component1992562
display id: component1992562
version: 1
top level: oriTr/LacZ
access: public
definition: BBa_J33207

BBa_J33207
type: SequenceAnnotation
persistent identity: annotation1992562
display id: annotation1992562
version: 1
top level: oriTr/LacZ
location: https://synbiohub.org/public/igem/BBa_K125350/annotation1992562/range1992562/1
component: BBa_J33207

https://synbiohub.org/public/igem/BBa_K125350/annotation1992562/range1992562/1
type: Range
persistent identity: range1992562
display id: range1992562
version: 1
top level: oriTr/LacZ
direction: direction/reverse
start: 1
end: 600
orientation: inline

BBa_J01003
type: SequenceAnnotation
persistent identity: annotation1992563
display id: annotation1992563
version: 1
top level: oriTr/LacZ
location: https://synbiohub.org/public/igem/BBa_K125350/annotation1992563/range1992563/1
component: BBa_J01003

https://synbiohub.org/public/igem/BBa_K125350/annotation1992563/range1992563/1
type: Range
persistent identity: range1992563
display id: range1992563
version: 1
top level: oriTr/LacZ
direction: direction/reverse
start: 609
end: 979
orientation: inline

Active Com
type: ComponentDefinition
persistent identity: BBa K2054006
display id: BBa_K2054006
version: 1
was generated by: iGEM to SBOL conversion
description: All Oligos for simplified Tetrahedral Nanostructure
creator: LAI HEI WAI
created: 2016-10-13T11:00:00Z
modified: 2016-10-27T11:54:39Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2522_
m user id: 30316
owner id: 30316
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This will be used to build and express our desired functional nanostructure in E. coli that target micro RNA biomarkers, but a simplified 2-strand nanostructure. This plasmid encodes the two ssDNA that will make the nanostructure, i.e. Oligos 1 and 5, from parts BBa_K2054001 and BBa_K2054005 respectively.
mutable notes: The assembly of the structure.
mutable provenance: Elbaz, J., Yin, P., & Voigt, C. A. (2016). Genetic encoding of DNA nanostructures and their self-assembly in living bacteria. Nature communications, 7.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_K2054006_sequence/1

BBa_K1614013
type: ComponentDefinition
persistent identity: BBa K1614013
display id: BBa_K1614013
version: 1
was generated by: iGEM to SBOL conversion
description: software generated kanamycin aptamer (candidate 2)
creator: Stefan Holderbach
created: 2015-09-15T11:00:00Z
modified: 2015-09-16T04:11:20Z
discontinued: false
dominant: false
group u list: _2031_
m user id: 12380
owner id: 12380
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Aptamer binding kanamycin created by the MAWS Software from iGEM Team Heidelberg
mutable notes: MAWS software from iGEM Team Heidelberg (created with the pre-alpha version)
mutable provenance: For further details on the in silico aptamer generation see:
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: Kanamycin aptamer
sequence: https://synbiohub.org/public/igem/BBa_K1614013_sequence/1

Kanamycin aptamer
type: SequenceAnnotation
persistent identity: annotation2458969
display id: annotation2458969
version: 1
top level: BBa_K1614013
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1614013/annotation2458969/range2458969/1

https://synbiohub.org/public/igem/BBa_K1614013/annotation2458969/range2458969/1
type: Range
persistent identity: range2458969
display id: range2458969
version: 1
direction: direction/forward
top level: BBa_K1614013
start: 1
end: 7
orientation: inline

BBa_K1680015
type: ComponentDefinition
persistent identity: BBa K1680015
display id: BBa_K1680015
version: 1
was generated by: iGEM to SBOL conversion
description: pRS315 yeast shuttle vector with Biobrick MCS
creator: Katharina Sporbeck
created: 2015-09-16T11:00:00Z
modified: 2015-10-28T06:36:51Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2098_
m user id: 26366
owner id: 26460
owning group id: 9
status: status/Planning
bookmark: false
mutable description: A Shuttle vector for yeast. The vector is compatible with RFC10 standard and contains an ADH terminator behind the RFC10 cloning site. Ampicilin resistance for bakteria and URA3 gene for yeast selection.
mutable notes: contains an RFC10 compatible multiple cloning site, no restrictions sites for RFC10 in the Backbone. ADH Terminator after MCS.
mutable provenance: yeast shuttle vector
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: T7 promotor
sequence annotation: RFC10
sequence annotation: ampR promotor
sequence annotation: lacZalpha
sequence annotation: pUC origin
sequence annotation: ampR
sequence annotation: LEU2 promotor
sequence annotation: f1 origin
sequence annotation: lac promotor
sequence annotation: T3 promotor
sequence annotation: CEN/ARS yeast centromere
sequence annotation: LEU2
sequence: https://synbiohub.org/public/igem/BBa_K1680015_sequence/1

T7 promotor
type: SequenceAnnotation
persistent identity: annotation2466192
display id: annotation2466192
version: 1
top level: BBa_K1680015
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1680015/annotation2466192/range2466192/1

https://synbiohub.org/public/igem/BBa_K1680015/annotation2466192/range2466192/1
type: Range
persistent identity: range2466192
display id: range2466192
version: 1
direction: direction/forward
top level: BBa_K1680015
start: 3097
end: 3115
orientation: inline

RFC10
type: SequenceAnnotation
persistent identity: annotation2466193
display id: annotation2466193
version: 1
top level: BBa_K1680015
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1680015/annotation2466193/range2466193/1

https://synbiohub.org/public/igem/BBa_K1680015/annotation2466193/range2466193/1
type: Range
persistent identity: range2466193
display id: range2466193
version: 1
direction: direction/forward
top level: BBa_K1680015
start: 3134
end: 3176
orientation: inline

ampR promotor
type: SequenceAnnotation
persistent identity: annotation2466202
display id: annotation2466202
version: 1
top level: BBa_K1680015
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1680015/annotation2466202/range2466202/1

https://synbiohub.org/public/igem/BBa_K1680015/annotation2466202/range2466202/1
type: Range
persistent identity: range2466202
display id: range2466202
version: 1
direction: direction/reverse
top level: BBa_K1680015
start: 5266
end: 5370
orientation: inline

lacZalpha
type: SequenceAnnotation
persistent identity: annotation2466196
display id: annotation2466196
version: 1
top level: BBa_K1680015
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1680015/annotation2466196/range2466196/1

https://synbiohub.org/public/igem/BBa_K1680015/annotation2466196/range2466196/1
type: Range
persistent identity: range2466196
display id: range2466196
version: 1
direction: direction/reverse
top level: BBa_K1680015
start: 3186
end: 3248
orientation: inline

pUC origin
type: SequenceAnnotation
persistent identity: annotation2466200
display id: annotation2466200
version: 1
top level: BBa_K1680015
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1680015/annotation2466200/range2466200/1

https://synbiohub.org/public/igem/BBa_K1680015/annotation2466200/range2466200/1
type: Range
persistent identity: range2466200
display id: range2466200
version: 1
direction: direction/reverse
top level: BBa_K1680015
start: 3646
end: 4234
orientation: inline

ampR
type: SequenceAnnotation
persistent identity: annotation2466201
display id: annotation2466201
version: 1
top level: BBa_K1680015
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1680015/annotation2466201/range2466201/1

https://synbiohub.org/public/igem/BBa_K1680015/annotation2466201/range2466201/1
type: Range
persistent identity: range2466201
display id: range2466201
version: 1
direction: direction/reverse
top level: BBa_K1680015
start: 4405
end: 5265
orientation: inline

LEU2 promotor
type: SequenceAnnotation
persistent identity: annotation2466189
display id: annotation2466189
version: 1
top level: BBa_K1680015
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1680015/annotation2466189/range2466189/1

https://synbiohub.org/public/igem/BBa_K1680015/annotation2466189/range2466189/1
type: Range
persistent identity: range2466189
display id: range2466189
version: 1
direction: direction/reverse
top level: BBa_K1680015
start: 1770
end: 2174
orientation: inline

f1 origin
type: SequenceAnnotation
persistent identity: annotation2466190
display id: annotation2466190
version: 1
top level: BBa_K1680015
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1680015/annotation2466190/range2466190/1

https://synbiohub.org/public/igem/BBa_K1680015/annotation2466190/range2466190/1
type: Range
persistent identity: range2466190
display id: range2466190
version: 1
direction: direction/reverse
top level: BBa_K1680015
start: 2474
end: 2929
orientation: inline

lacZalpha
type: SequenceAnnotation
persistent identity: annotation2466191
display id: annotation2466191
version: 1
top level: BBa_K1680015
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1680015/annotation2466191/range2466191/1

https://synbiohub.org/public/igem/BBa_K1680015/annotation2466191/range2466191/1
type: Range
persistent identity: range2466191
display id: range2466191
version: 1
direction: direction/reverse
top level: BBa_K1680015
start: 2712
end: 3128
orientation: inline

lac promotor
type: SequenceAnnotation
persistent identity: annotation2466199
display id: annotation2466199
version: 1
top level: BBa_K1680015
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1680015/annotation2466199/range2466199/1

https://synbiohub.org/public/igem/BBa_K1680015/annotation2466199/range2466199/1
type: Range
persistent identity: range2466199
display id: range2466199
version: 1
direction: direction/reverse
top level: BBa_K1680015
start: 3292
end: 3322
orientation: inline

T3 promotor
type: SequenceAnnotation
persistent identity: annotation2466197
display id: annotation2466197
version: 1
top level: BBa_K1680015
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1680015/annotation2466197/range2466197/1

https://synbiohub.org/public/igem/BBa_K1680015/annotation2466197/range2466197/1
type: Range
persistent identity: range2466197
display id: range2466197
version: 1
direction: direction/reverse
top level: BBa_K1680015
start: 3205
end: 3223
orientation: inline

CEN/ARS yeast centromere
type: SequenceAnnotation
persistent identity: annotation2466204
display id: annotation2466204
version: 1
top level: BBa_K1680015
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1680015/annotation2466204/range2466204/1

https://synbiohub.org/public/igem/BBa_K1680015/annotation2466204/range2466204/1
type: Range
persistent identity: range2466204
display id: range2466204
version: 1
direction: direction/forward
top level: BBa_K1680015
start: 5407
end: 5910
orientation: inline

LEU2
type: SequenceAnnotation
persistent identity: annotation2466188
display id: annotation2466188
version: 1
top level: BBa_K1680015
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1680015/annotation2466188/range2466188/1

https://synbiohub.org/public/igem/BBa_K1680015/annotation2466188/range2466188/1
type: Range
persistent identity: range2466188
display id: range2466188
version: 1
direction: direction/reverse
top level: BBa_K1680015
start: 663
end: 1757
orientation: inline

BioScaffol
type: ComponentDefinition
persistent identity: BBa K259004
display id: BBa_K259004
version: 1
was generated by: iGEM to SBOL conversion
description: BioScaffold Linker - Removes Stop Codons & scars & replaces with a Gly-Ser linker
created: 2009-07-31T11:00:00Z
modified: 2015-05-08T01:11:42Z
mutable provenance: This part is completely designed ''de novo''. This sequence does not occur naturally.
owned by: james
owned by: myers
mutable description: This part is part of a family of Bioscaffold - Linkers. This part will help users interested in protein-fusion. The usage of the part will allow in frame assembly of 2(+) proteins by: *Removing the stop codons from the upstream protein. *Removing the scar created by the Assembly Standard 10 (RFC10) construction of 2(+) protein coding sequences. *Maintaining the start codon of the downstream protein. *Adding a flexible (Gly-Ser-Gly) linker between the two protein coding sequences. *Acting as a translational buffer if ligation is incorrect. ===Assembly Instructions=== *Create an Assembly Standard 10 fusion as below ; **Upstream Protein Part - Bioscaffold Linker - Downstream Protein Part** *Use the BioScaffold Specific Enzymes (i)Restrict with BpuEI to remove upstream scar (created between Upstream Protein - Bioscaffold) and stop codons from upstream protein part. (ii)Ligate and you will get the intermediate product; **Upstream Protein (w/out Stop codons) - Tyr - BioScaffold Linker - Downstream Protein** *Restrict with CspCI to remove downstream scar. *Ligate to get the final product; **Upstream Protein - Tyr-Gly-Ser-Gly- Downstream Protein**
discontinued: false
dominant: false
experience: experience/None
group u list: _357_
m user id: 0
owner id: 5318
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: This part was designed taking into consideration the requirements by Assembly Standard 10 (RFC10).
star: false
creator: Petros Mina
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: BpuEI
sequence annotation: Translational Buffer
sequence annotation: CspCI (5' Recognition Seq)
sequence annotation: Gly-Ser-Gly Linker
sequence annotation: Tyr Involved in Restriction/Ligation
sequence annotation: Met Involved in Restriction/Ligation
sequence annotation: CspCI (3' Recognition Seq)
sequence: https://synbiohub.org/public/igem/BBa_K259004_sequence/1

BpuEI
type: SequenceAnnotation
persistent identity: annotation2014598
display id: annotation2014598
version: 1
top level: BioScaffol
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K259004/annotation2014598/range2014598/1

https://synbiohub.org/public/igem/BBa_K259004/annotation2014598/range2014598/1
type: Range
persistent identity: range2014598
display id: range2014598
version: 1
top level: BioScaffol
direction: direction/forward
start: 21
end: 26
orientation: inline

Translational Buffer
type: SequenceAnnotation
persistent identity: annotation2014604
display id: annotation2014604
version: 1
top level: BioScaffol
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K259004/annotation2014604/range2014604/1

https://synbiohub.org/public/igem/BBa_K259004/annotation2014604/range2014604/1
type: Range
persistent identity: range2014604
display id: range2014604
version: 1
top level: BioScaffol
direction: direction/forward
start: 70
end: 72
orientation: inline

Translational Buffer
type: SequenceAnnotation
persistent identity: annotation2014602
display id: annotation2014602
version: 1
top level: BioScaffol
role: feature/stop
role: SO:0000319
location: https://synbiohub.org/public/igem/BBa_K259004/annotation2014602/range2014602/1

https://synbiohub.org/public/igem/BBa_K259004/annotation2014602/range2014602/1
type: Range
persistent identity: range2014602
display id: range2014602
version: 1
top level: BioScaffol
direction: direction/forward
start: 56
end: 65
orientation: inline

CspCI (5' Recognition Seq)
type: SequenceAnnotation
persistent identity: annotation2014603
display id: annotation2014603
version: 1
top level: BioScaffol
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K259004/annotation2014603/range2014603/1

https://synbiohub.org/public/igem/BBa_K259004/annotation2014603/range2014603/1
type: Range
persistent identity: range2014603
display id: range2014603
version: 1
top level: BioScaffol
direction: direction/forward
start: 66
end: 68
orientation: inline

Gly-Ser-Gly Linker
type: SequenceAnnotation
persistent identity: annotation2014600
display id: annotation2014600
version: 1
top level: BioScaffol
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K259004/annotation2014600/range2014600/1

https://synbiohub.org/public/igem/BBa_K259004/annotation2014600/range2014600/1
type: Range
persistent identity: range2014600
display id: range2014600
version: 1
top level: BioScaffol
direction: direction/forward
start: 44
end: 52
orientation: inline

Tyr Involved in Restriction/Ligation
type: SequenceAnnotation
persistent identity: annotation2014599
display id: annotation2014599
version: 1
top level: BioScaffol
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K259004/annotation2014599/range2014599/1

https://synbiohub.org/public/igem/BBa_K259004/annotation2014599/range2014599/1
type: Range
persistent identity: range2014599
display id: range2014599
version: 1
top level: BioScaffol
direction: direction/forward
start: 41
end: 43
orientation: inline

Met Involved in Restriction/Ligation
type: SequenceAnnotation
persistent identity: annotation2014601
display id: annotation2014601
version: 1
top level: BioScaffol
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K259004/annotation2014601/range2014601/1

https://synbiohub.org/public/igem/BBa_K259004/annotation2014601/range2014601/1
type: Range
persistent identity: range2014601
display id: range2014601
version: 1
top level: BioScaffol
direction: direction/forward
start: 53
end: 55
orientation: inline

CspCI (3' Recognition Seq)
type: SequenceAnnotation
persistent identity: annotation2014605
display id: annotation2014605
version: 1
top level: BioScaffol
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K259004/annotation2014605/range2014605/1

https://synbiohub.org/public/igem/BBa_K259004/annotation2014605/range2014605/1
type: Range
persistent identity: range2014605
display id: range2014605
version: 1
top level: BioScaffol
direction: direction/forward
start: 74
end: 77
orientation: inline

BpuEI
type: SequenceAnnotation
persistent identity: annotation2014597
display id: annotation2014597
version: 1
top level: BioScaffol
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K259004/annotation2014597/range2014597/1

https://synbiohub.org/public/igem/BBa_K259004/annotation2014597/range2014597/1
type: Range
persistent identity: range2014597
display id: range2014597
version: 1
top level: BioScaffol
direction: direction/reverse
start: 3
end: 8
orientation: inline

I-SceI RS
type: ComponentDefinition
persistent identity: BBa K175027
display id: BBa_K175027
version: 1
was generated by: iGEM to SBOL conversion
description: I-SceI restriction site
created: 2009-07-31T11:00:00Z
modified: 2015-05-08T01:10:59Z
mutable provenance: synthesised
owned by: james
owned by: myers
mutable description: I-SceI homing endonuclease site Restriction site for the homing endonuclease I-SceI. Sequence will be cut as follows: 5' A G T T A C G C T A G G G A T A A^C A G G G T A A T A T A G 3' 3' T C A A T G C G A T C C C^T A T T G T C C C A T T A T A T C 5'
discontinued: false
dominant: false
experience: experience/Works
group u list: _280_
m user id: 0
owner id: 4302
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: Design was very simple. The recognition site was copied from NEB and synthesised
star: false
creator: Tim Weenink
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: I-SceI homing endonuclease restriction site
sequence: https://synbiohub.org/public/igem/BBa_K175027_sequence/1

I-SceI homing endonuclease restriction site
type: SequenceAnnotation
persistent identity: annotation2014585
display id: annotation2014585
version: 1
top level: I-SceI RS
role: feature/dna
role: SO:0000110
location: https://synbiohub.org/public/igem/BBa_K175027/annotation2014585/range2014585/1

https://synbiohub.org/public/igem/BBa_K175027/annotation2014585/range2014585/1
type: Range
persistent identity: range2014585
display id: range2014585
version: 1
top level: I-SceI RS
direction: direction/forward
start: 1
end: 30
orientation: inline

VF2
type: ComponentDefinition
persistent identity: BBa G00100
display id: BBa_G00100
version: 1
was generated by: iGEM to SBOL conversion
description: Forward primer for sequencing/amplifying BioBrick parts (VF2)
created: 2004-05-24T11:00:00Z
modified: 2015-08-31T04:07:28Z
owned by: james
owned by: myers
mutable description: Originally named VF2, primer binds upstream of the standard BBa_MCS on biobrick vectors.
discontinued: false
dominant: false
experience: experience/Works
group u list: _11_1_
m user id: 0
owner id: 60
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Tom Knight
type: DnaRegion
role: SO:0000112
role: partType/Primer
sequence annotation: VF2
sequence: https://synbiohub.org/public/igem/BBa_G00100_sequence/1

VF2
type: SequenceAnnotation
persistent identity: annotation1934505
display id: annotation1934505
version: 1
top level: VF2
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_G00100/annotation1934505/range1934505/1

https://synbiohub.org/public/igem/BBa_G00100/annotation1934505/range1934505/1
type: Range
persistent identity: range1934505
display id: range1934505
version: 1
top level: VF2
direction: direction/forward
start: 1
end: 20
orientation: inline

BsiWI
type: ComponentDefinition
persistent identity: BBa M31996
display id: BBa_M31996
version: 1
was generated by: iGEM to SBOL conversion
description: BsiWI restriction enzyme site
created: 2007-02-27T12:00:00Z
modified: 2015-05-08T01:14:01Z
mutable provenance: A E. coli strain that carries the BsiWI gene from Bacillus species (D. Clark).
owned by: james
owned by: myers
mutable description: BsiWI restriction enzyme site
discontinued: false
dominant: false
experience: experience/None
group u list: _102_
m user id: 0
owner id: 1373
owning group id: 102
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: none
star: false
creator: Tiffany Guo
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_M31996_sequence/1

tetO array
type: ComponentDefinition
persistent identity: BBa K606026
display id: BBa_K606026
version: 1
was generated by: iGEM to SBOL conversion
description: tetO Array
created: 2011-07-26T11:00:00Z
modified: 2015-05-08T01:12:51Z
mutable provenance: Andrew Wright, Dave Lane, Fran??ois-Xavier Barre, pDAG479 plasmid.
owned by: james
owned by: myers
mutable description: tetO Array Contains lots of tetO where bind YFP:tetR (<partinfo>BBa_K606025</partinfo>).
discontinued: false
dominant: false
experience: experience/Works
group u list: _778_
m user id: 0
owner id: 8972
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: Reduce sequence to avoid restriction sites
star: false
creator: K??vin Yauy
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: tetO array
sequence: https://synbiohub.org/public/igem/BBa_K606026_sequence/1

tetO array
type: SequenceAnnotation
persistent identity: annotation2124086
display id: annotation2124086
version: 1
top level: tetO array
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K606026/annotation2124086/range2124086/1

https://synbiohub.org/public/igem/BBa_K606026/annotation2124086/range2124086/1
type: Range
persistent identity: range2124086
display id: range2124086
version: 1
top level: tetO array
direction: direction/forward
start: 1
end: 3628
orientation: inline

K592200
type: ComponentDefinition
persistent identity: BBa K592200
display id: BBa_K592200
version: 1
was generated by: iGEM to SBOL conversion
description: Low to medium copy BioBrick standard vector
created: 2011-09-20T11:00:00Z
modified: 2015-05-08T01:12:49Z
mutable provenance: Salmonella phage P22 late terminator.
owned by: james
owned by: myers
mutable description: BBa_K592200 is a BioBrick standard vector with low to medium copy p15A replication origin (BBa_I50032) and spectinomycin antibiotic resistance marker, usable for Lambda Red recombineering in E coli. The E coli His operon terminator BBa_B0053 has been replaced with the late terminator of the Salmonella phage P22.
discontinued: false
dominant: false
experience: experience/Works
group u list: _763_
m user id: 0
owner id: 10133
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: To make the plasmid usable for Lambda Red recombineering, all sequences with homologies to the E coli chromosome had to be removed. To accomplish this, the E coli His operon terminator BBa_B0053 has been replaced with the late terminator of the Salmonella phage P22.
star: false
creator: Erik Gullberg, Antonio Ascue Avalos, Erik Lundin
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: SpcR promoter
sequence annotation: Spectinomycin resistance marker
sequence annotation: Translational stop BBa_B0042
sequence annotation: VF2 site BBa_G00100
sequence annotation: Terminator BBa_B0062
sequence annotation: VR site BBa_G00102
sequence annotation: T3 terminator BBa_B0055
sequence annotation: Lambda Red R primer
sequence annotation: p15A low to medium copy replication origin BBa_I50032
sequence annotation: P22 late terminator
sequence annotation: Lambda red F primer
sequence annotation: Terminator BBa_B0054
sequence: https://synbiohub.org/public/igem/BBa_K592200_sequence/1

SpcR promoter
type: SequenceAnnotation
persistent identity: annotation2136376
display id: annotation2136376
version: 1
top level: K592200
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K592200/annotation2136376/range2136376/1

https://synbiohub.org/public/igem/BBa_K592200/annotation2136376/range2136376/1
type: Range
persistent identity: range2136376
display id: range2136376
version: 1
top level: K592200
direction: direction/reverse
start: 2907
end: 2933
orientation: inline

Spectinomycin resistance marker
type: SequenceAnnotation
persistent identity: annotation2136375
display id: annotation2136375
version: 1
top level: K592200
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K592200/annotation2136375/range2136375/1

https://synbiohub.org/public/igem/BBa_K592200/annotation2136375/range2136375/1
type: Range
persistent identity: range2136375
display id: range2136375
version: 1
top level: K592200
direction: direction/reverse
start: 1841
end: 2840
orientation: inline

Translational stop BBa_B0042
type: SequenceAnnotation
persistent identity: annotation2136380
display id: annotation2136380
version: 1
top level: K592200
role: feature/stop
role: SO:0000319
location: https://synbiohub.org/public/igem/BBa_K592200/annotation2136380/range2136380/1

https://synbiohub.org/public/igem/BBa_K592200/annotation2136380/range2136380/1
type: Range
persistent identity: range2136380
display id: range2136380
version: 1
top level: K592200
direction: direction/forward
start: 3091
end: 3102
orientation: inline

VF2 site BBa_G00100
type: SequenceAnnotation
persistent identity: annotation2136378
display id: annotation2136378
version: 1
top level: K592200
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K592200/annotation2136378/range2136378/1

https://synbiohub.org/public/igem/BBa_K592200/annotation2136378/range2136378/1
type: Range
persistent identity: range2136378
display id: range2136378
version: 1
top level: K592200
direction: direction/forward
start: 2993
end: 3012
orientation: inline

Terminator BBa_B0062
type: SequenceAnnotation
persistent identity: annotation2136372
display id: annotation2136372
version: 1
top level: K592200
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K592200/annotation2136372/range2136372/1

https://synbiohub.org/public/igem/BBa_K592200/annotation2136372/range2136372/1
type: Range
persistent identity: range2136372
display id: range2136372
version: 1
top level: K592200
direction: direction/forward
start: 136
end: 176
orientation: inline

VR site BBa_G00102
type: SequenceAnnotation
persistent identity: annotation2136371
display id: annotation2136371
version: 1
top level: K592200
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K592200/annotation2136371/range2136371/1

https://synbiohub.org/public/igem/BBa_K592200/annotation2136371/range2136371/1
type: Range
persistent identity: range2136371
display id: range2136371
version: 1
top level: K592200
direction: direction/reverse
start: 116
end: 135
orientation: inline

T3 terminator BBa_B0055
type: SequenceAnnotation
persistent identity: annotation2136379
display id: annotation2136379
version: 1
top level: K592200
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K592200/annotation2136379/range2136379/1

https://synbiohub.org/public/igem/BBa_K592200/annotation2136379/range2136379/1
type: Range
persistent identity: range2136379
display id: range2136379
version: 1
top level: K592200
direction: direction/forward
start: 3013
end: 3090
orientation: inline

Lambda Red R primer
type: SequenceAnnotation
persistent identity: annotation2136398
display id: annotation2136398
version: 1
top level: K592200
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K592200/annotation2136398/range2136398/1

https://synbiohub.org/public/igem/BBa_K592200/annotation2136398/range2136398/1
type: Range
persistent identity: range2136398
display id: range2136398
version: 1
top level: K592200
direction: direction/reverse
start: 20
end: 41
orientation: inline

Translational stop BBa_B0042
type: SequenceAnnotation
persistent identity: annotation2136369
display id: annotation2136369
version: 1
top level: K592200
role: feature/stop
role: SO:0000319
location: https://synbiohub.org/public/igem/BBa_K592200/annotation2136369/range2136369/1

https://synbiohub.org/public/igem/BBa_K592200/annotation2136369/range2136369/1
type: Range
persistent identity: range2136369
display id: range2136369
version: 1
top level: K592200
direction: direction/forward
start: 35
end: 46
orientation: inline

p15A low to medium copy replication origin BBa_I50032
type: SequenceAnnotation
persistent identity: annotation2136373
display id: annotation2136373
version: 1
top level: K592200
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K592200/annotation2136373/range2136373/1

https://synbiohub.org/public/igem/BBa_K592200/annotation2136373/range2136373/1
type: Range
persistent identity: range2136373
display id: range2136373
version: 1
top level: K592200
direction: direction/forward
start: 184
end: 1748
orientation: inline

P22 late terminator
type: SequenceAnnotation
persistent identity: annotation2136377
display id: annotation2136377
version: 1
top level: K592200
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K592200/annotation2136377/range2136377/1

https://synbiohub.org/public/igem/BBa_K592200/annotation2136377/range2136377/1
type: Range
persistent identity: range2136377
display id: range2136377
version: 1
top level: K592200
direction: direction/forward
start: 2952
end: 2992
orientation: inline

Lambda red F primer
type: SequenceAnnotation
persistent identity: annotation2136374
display id: annotation2136374
version: 1
top level: K592200
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K592200/annotation2136374/range2136374/1

https://synbiohub.org/public/igem/BBa_K592200/annotation2136374/range2136374/1
type: Range
persistent identity: range2136374
display id: range2136374
version: 1
top level: K592200
direction: direction/forward
start: 1726
end: 1745
orientation: inline

Terminator BBa_B0054
type: SequenceAnnotation
persistent identity: annotation2136370
display id: annotation2136370
version: 1
top level: K592200
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K592200/annotation2136370/range2136370/1

https://synbiohub.org/public/igem/BBa_K592200/annotation2136370/range2136370/1
type: Range
persistent identity: range2136370
display id: range2136370
version: 1
top level: K592200
direction: direction/forward
start: 47
end: 115
orientation: inline

4-fingers
type: ComponentDefinition
persistent identity: BBa J35004
display id: BBa_J35004
version: 1
was generated by: iGEM to SBOL conversion
description: 4-fingers arm
created: 2006-10-22T11:00:00Z
modified: 2015-08-31T04:08:47Z
mutable provenance: artificial sequence
owned by: james
owned by: myers
mutable description: DNA ORIGAMI BioBrick
discontinued: false
dominant: false
experience: experience/None
group u list: _64_
m user id: 0
owner id: 807
owning group id: 64
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: OligoAnalyzer 3.0 http://www.idtdna.com/analyzer/Applications/OligoAnalyzer/
star: false
creator: Andrey Kuznetsov
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_J35004_sequence/1

SacII
type: ComponentDefinition
persistent identity: BBa I11078
display id: BBa_I11078
version: 1
was generated by: iGEM to SBOL conversion
description: SacII restriction enzyme site with extra flanking nucleotides
created: 2004-09-06T11:00:00Z
modified: 2015-08-31T04:07:30Z
mutable provenance: NEB catalog
owned by: james
owned by: myers
mutable description: SacII restriction site
discontinued: false
dominant: false
experience: experience/None
group u list: _2_
m user id: 0
owner id: 102
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: bu
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_I11078_sequence/1

BBa_J56011
type: ComponentDefinition
persistent identity: BBa J56011
display id: BBa_J56011
version: 1
was generated by: iGEM to SBOL conversion
description: MCSII
created: 2006-10-31T12:00:00Z
modified: 2015-08-31T03:22:59Z
mutable provenance: Synthesized by DNA 2.0
owned by: james
owned by: myers
mutable description: MCSII
discontinued: false
dominant: false
experience: experience/None
group u list: _79_98_
m user id: 0
owner id: 1132
owning group id: 98
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: None
star: false
creator: Matt Eames
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_J56011_sequence/1

Scar 25
type: ComponentDefinition
persistent identity: BBa B0105
display id: BBa_B0105
version: 1
was generated by: iGEM to SBOL conversion
description: RFC 25 Scar Sequence
created: 2009-10-14T11:00:00Z
modified: 2015-08-31T04:07:21Z
mutable provenance: BBF RFC 25
owned by: james
owned by: myers
mutable description: This is the scar produced by assembly using RFC 25. If you are assembling a composite part using RFC 25, you can insert this part and specify blunt assembly to get the desired sequence.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 25
owning group id: 397
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Simple DNA sequence
star: false
creator: Randy Rettberg
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: Scar 25
sequence: https://synbiohub.org/public/igem/BBa_B0105_sequence/1

Scar 25
type: SequenceAnnotation
persistent identity: annotation2041621
display id: annotation2041621
version: 1
top level: Scar 25
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_B0105/annotation2041621/range2041621/1

https://synbiohub.org/public/igem/BBa_B0105/annotation2041621/range2041621/1
type: Range
persistent identity: range2041621
display id: range2041621
version: 1
top level: Scar 25
direction: direction/forward
start: 1
end: 6
orientation: inline

BBa_K1680016
type: ComponentDefinition
persistent identity: BBa K1680016
display id: BBa_K1680016
version: 1
was generated by: iGEM to SBOL conversion
description: pRS316 yeast shuttle vector with Biobrick MCS
creator: Katharina Sporbeck
created: 2015-09-16T11:00:00Z
modified: 2015-10-28T06:37:03Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2098_
m user id: 26366
owner id: 26460
owning group id: 9
status: status/Planning
bookmark: false
mutable description: A Shuttle vector for yeast. The vector is compatible with RFC10 standard and contains an ADH terminator behind the RFC10 cloning site. Ampicilin resistance for bacteria and URA3 gene for yeast selection.
mutable notes: Has an RFC10 compatible MCS, with ADH Terminator. No further RFC10 restrictions sites in the backbone.
mutable provenance: yeast shuttle vector
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: CEN/ARS yeast centromere
sequence annotation: URA3 promotor
sequence annotation: ampR
sequence annotation: URA3
sequence annotation: pUC origin
sequence annotation: f1 origin
sequence annotation: T7 promotor
sequence annotation: lacZalpha
sequence annotation: ampR promotor
sequence annotation: T3 promotor
sequence annotation: lac promotor
sequence annotation: RFC10 MCS
sequence: https://synbiohub.org/public/igem/BBa_K1680016_sequence/1

CEN/ARS yeast centromere
type: SequenceAnnotation
persistent identity: annotation2466237
display id: annotation2466237
version: 1
top level: BBa_K1680016
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1680016/annotation2466237/range2466237/1

https://synbiohub.org/public/igem/BBa_K1680016/annotation2466237/range2466237/1
type: Range
persistent identity: range2466237
display id: range2466237
version: 1
direction: direction/forward
top level: BBa_K1680016
start: 4276
end: 4779
orientation: inline

URA3 promotor
type: SequenceAnnotation
persistent identity: annotation2466206
display id: annotation2466206
version: 1
top level: BBa_K1680016
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1680016/annotation2466206/range2466206/1

https://synbiohub.org/public/igem/BBa_K1680016/annotation2466206/range2466206/1
type: Range
persistent identity: range2466206
display id: range2466206
version: 1
direction: direction/forward
top level: BBa_K1680016
start: 195
end: 410
orientation: inline

ampR
type: SequenceAnnotation
persistent identity: annotation2466235
display id: annotation2466235
version: 1
top level: BBa_K1680016
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1680016/annotation2466235/range2466235/1

https://synbiohub.org/public/igem/BBa_K1680016/annotation2466235/range2466235/1
type: Range
persistent identity: range2466235
display id: range2466235
version: 1
direction: direction/reverse
top level: BBa_K1680016
start: 3274
end: 4134
orientation: inline

URA3
type: SequenceAnnotation
persistent identity: annotation2466209
display id: annotation2466209
version: 1
top level: BBa_K1680016
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1680016/annotation2466209/range2466209/1

https://synbiohub.org/public/igem/BBa_K1680016/annotation2466209/range2466209/1
type: Range
persistent identity: range2466209
display id: range2466209
version: 1
direction: direction/forward
top level: BBa_K1680016
start: 411
end: 1214
orientation: inline

pUC origin
type: SequenceAnnotation
persistent identity: annotation2466222
display id: annotation2466222
version: 1
top level: BBa_K1680016
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1680016/annotation2466222/range2466222/1

https://synbiohub.org/public/igem/BBa_K1680016/annotation2466222/range2466222/1
type: Range
persistent identity: range2466222
display id: range2466222
version: 1
direction: direction/reverse
top level: BBa_K1680016
start: 2515
end: 3103
orientation: inline

f1 origin
type: SequenceAnnotation
persistent identity: annotation2466211
display id: annotation2466211
version: 1
top level: BBa_K1680016
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1680016/annotation2466211/range2466211/1

https://synbiohub.org/public/igem/BBa_K1680016/annotation2466211/range2466211/1
type: Range
persistent identity: range2466211
display id: range2466211
version: 1
direction: direction/reverse
top level: BBa_K1680016
start: 1343
end: 1798
orientation: inline

T7 promotor
type: SequenceAnnotation
persistent identity: annotation2466214
display id: annotation2466214
version: 1
top level: BBa_K1680016
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1680016/annotation2466214/range2466214/1

https://synbiohub.org/public/igem/BBa_K1680016/annotation2466214/range2466214/1
type: Range
persistent identity: range2466214
display id: range2466214
version: 1
direction: direction/forward
top level: BBa_K1680016
start: 1966
end: 1985
orientation: inline

lacZalpha
type: SequenceAnnotation
persistent identity: annotation2466217
display id: annotation2466217
version: 1
top level: BBa_K1680016
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1680016/annotation2466217/range2466217/1

https://synbiohub.org/public/igem/BBa_K1680016/annotation2466217/range2466217/1
type: Range
persistent identity: range2466217
display id: range2466217
version: 1
direction: direction/reverse
top level: BBa_K1680016
start: 2055
end: 2117
orientation: inline

ampR promotor
type: SequenceAnnotation
persistent identity: annotation2466236
display id: annotation2466236
version: 1
top level: BBa_K1680016
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1680016/annotation2466236/range2466236/1

https://synbiohub.org/public/igem/BBa_K1680016/annotation2466236/range2466236/1
type: Range
persistent identity: range2466236
display id: range2466236
version: 1
direction: direction/reverse
top level: BBa_K1680016
start: 4135
end: 4239
orientation: inline

T3 promotor
type: SequenceAnnotation
persistent identity: annotation2466219
display id: annotation2466219
version: 1
top level: BBa_K1680016
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1680016/annotation2466219/range2466219/1

https://synbiohub.org/public/igem/BBa_K1680016/annotation2466219/range2466219/1
type: Range
persistent identity: range2466219
display id: range2466219
version: 1
direction: direction/reverse
top level: BBa_K1680016
start: 2073
end: 2092
orientation: inline

lac promotor
type: SequenceAnnotation
persistent identity: annotation2466221
display id: annotation2466221
version: 1
top level: BBa_K1680016
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1680016/annotation2466221/range2466221/1

https://synbiohub.org/public/igem/BBa_K1680016/annotation2466221/range2466221/1
type: Range
persistent identity: range2466221
display id: range2466221
version: 1
direction: direction/reverse
top level: BBa_K1680016
start: 2161
end: 2191
orientation: inline

lacZalpha
type: SequenceAnnotation
persistent identity: annotation2466213
display id: annotation2466213
version: 1
top level: BBa_K1680016
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1680016/annotation2466213/range2466213/1

https://synbiohub.org/public/igem/BBa_K1680016/annotation2466213/range2466213/1
type: Range
persistent identity: range2466213
display id: range2466213
version: 1
direction: direction/reverse
top level: BBa_K1680016
start: 1581
end: 1997
orientation: inline

RFC10 MCS
type: SequenceAnnotation
persistent identity: annotation2466215
display id: annotation2466215
version: 1
top level: BBa_K1680016
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1680016/annotation2466215/range2466215/1

https://synbiohub.org/public/igem/BBa_K1680016/annotation2466215/range2466215/1
type: Range
persistent identity: range2466215
display id: range2466215
version: 1
direction: direction/reverse
top level: BBa_K1680016
start: 2003
end: 2045
orientation: inline

MDA
type: ComponentDefinition
persistent identity: BBa M36761
display id: BBa_M36761
version: 1
was generated by: iGEM to SBOL conversion
description: Melamine deaminase coding sequence
creator: Ray Paul Laureano-Mercado & Mark Kirollos
created: 2013-06-09T11:00:00Z
modified: 2015-05-08T01:14:06Z
discontinued: false
dominant: false
experience: experience/None
group u list: _848_
m user id: 0
owner id: 17944
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This part is the coding sequence for the enzyme melamine deaminase, optimized for usage in E. coli. This enzyme eliminates an amine group from melamine to produce ammonia and ammeline and is able to remove another amine group at a rate 10 times slower than the first reaction to bring the compound to ammelide. The enzyme does not react to a measurable extent with this last compound, so it metabolizes melamine by consecutive and predictable deaminations.
mutable notes: The TriA gene???s codons were modified from the original and optimized for E. coli to a Codon Adaptation Index (CAI) of .76. After that, some palindromic and mRNA destabilizing sequences were removed to increase the stability of our DNA during synthesis.
mutable provenance: The original gene coding for the protein of interest was isolated from a species of Pseudomonas (http://www.ncbi.nlm.nih.gov/nuccore/11890744).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Melamine Deaminase
sequence annotation: Stop (TAG)
sequence annotation: Start (ATG)
sequence: https://synbiohub.org/public/igem/BBa_M36761_sequence/1

Melamine Deaminase
type: SequenceAnnotation
persistent identity: annotation2329380
display id: annotation2329380
version: 1
top level: MDA
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_M36761/annotation2329380/range2329380/1

https://synbiohub.org/public/igem/BBa_M36761/annotation2329380/range2329380/1
type: Range
persistent identity: range2329380
display id: range2329380
version: 1
direction: direction/forward
top level: MDA
start: 1
end: 1422
orientation: inline

Stop (TAG)
type: SequenceAnnotation
persistent identity: annotation2329382
display id: annotation2329382
version: 1
top level: MDA
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_M36761/annotation2329382/range2329382/1

https://synbiohub.org/public/igem/BBa_M36761/annotation2329382/range2329382/1
type: Range
persistent identity: range2329382
display id: range2329382
version: 1
direction: direction/forward
top level: MDA
start: 1423
end: 1425
orientation: inline

Start (ATG)
type: SequenceAnnotation
persistent identity: annotation2329381
display id: annotation2329381
version: 1
top level: MDA
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_M36761/annotation2329381/range2329381/1

https://synbiohub.org/public/igem/BBa_M36761/annotation2329381/range2329381/1
type: Range
persistent identity: range2329381
display id: range2329381
version: 1
direction: direction/forward
top level: MDA
start: 1
end: 3
orientation: inline

BBa_K598001
type: ComponentDefinition
persistent identity: BBa K598001
display id: BBa_K598001
version: 1
was generated by: iGEM to SBOL conversion
description: Theophylline Responsive Riboswitch 1G1 with Engineered RBS+GFP generator
created: 2011-09-26T11:00:00Z
modified: 2015-05-08T01:12:50Z
mutable provenance: Theophylline riboswich is composed of a synthesized theophylline aptamer and a regulatory platform.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: This is a GFP generator with a theophylline responsive riboswitch sequence. The mRNA with a theophylline riboswitch in it would respond to theophylline concerntration, producing different amount of GFP. Under the control of different promoters, it could be used to demonstrate the theophylline responsive curve, providing data for modeling. The theophylline riboswitch sequence could also be used to regulate the translation of other genes.
discontinued: false
dominant: false
experience: experience/Works
group u list: _770_
m user id: 0
owner id: 8713
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable notes: The RBS of this device is altered into AGGAGGU. When changing the RBS, the anti-RBS sequence should be changed as well.
star: false
creator: Siyang HAO, Tong MU, Zheng LUO, Mengqi ZHANG
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: Engineered RBS
sequence annotation: BBa_B0010
sequence annotation: BBa_E0040
sequence annotation: stop
sequence annotation: Theophylline responsive riboswitch Theo 1G1
sequence annotation: BBa_B0012
sequence annotation: polya
sequence annotation: T7 TE
sequence annotation: eGFP
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_K598001_sequence/1

Engineered RBS
type: SequenceAnnotation
persistent identity: annotation2152702
display id: annotation2152702
version: 1
top level: BBa_K598001
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K598001/annotation2152702/range2152702/1

https://synbiohub.org/public/igem/BBa_K598001/annotation2152702/range2152702/1
type: Range
persistent identity: range2152702
display id: range2152702
version: 1
top level: BBa_K598001
direction: direction/forward
start: 75
end: 81
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2152882
display id: annotation2152882
version: 1
top level: BBa_K598001
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K598001/annotation2152882/range2152882/1

https://synbiohub.org/public/igem/BBa_K598001/annotation2152882/range2152882/1
type: Range
persistent identity: range2152882
display id: range2152882
version: 1
top level: BBa_K598001
direction: direction/forward
start: 815
end: 894
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation2152703
display id: annotation2152703
version: 1
top level: BBa_K598001
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K598001/annotation2152703/range2152703/1

https://synbiohub.org/public/igem/BBa_K598001/annotation2152703/range2152703/1
type: Range
persistent identity: range2152703
display id: range2152703
version: 1
top level: BBa_K598001
direction: direction/forward
start: 87
end: 806
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2152892
display id: annotation2152892
version: 1
top level: BBa_K598001
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K598001/annotation2152892/range2152892/1

https://synbiohub.org/public/igem/BBa_K598001/annotation2152892/range2152892/1
type: Range
persistent identity: range2152892
display id: range2152892
version: 1
top level: BBa_K598001
direction: direction/forward
start: 936
end: 936
orientation: inline

Theophylline responsive riboswitch Theo 1G1
type: SequenceAnnotation
persistent identity: annotation2152701
display id: annotation2152701
version: 1
top level: BBa_K598001
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K598001/annotation2152701/range2152701/1

https://synbiohub.org/public/igem/BBa_K598001/annotation2152701/range2152701/1
type: Range
persistent identity: range2152701
display id: range2152701
version: 1
top level: BBa_K598001
direction: direction/forward
start: 1
end: 86
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2152705
display id: annotation2152705
version: 1
top level: BBa_K598001
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K598001/annotation2152705/range2152705/1

https://synbiohub.org/public/igem/BBa_K598001/annotation2152705/range2152705/1
type: Range
persistent identity: range2152705
display id: range2152705
version: 1
top level: BBa_K598001
direction: direction/forward
start: 903
end: 943
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation2152887
display id: annotation2152887
version: 1
top level: BBa_K598001
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_K598001/annotation2152887/range2152887/1

https://synbiohub.org/public/igem/BBa_K598001/annotation2152887/range2152887/1
type: Range
persistent identity: range2152887
display id: range2152887
version: 1
top level: BBa_K598001
direction: direction/forward
start: 930
end: 943
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation2152901
display id: annotation2152901
version: 1
top level: BBa_K598001
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K598001/annotation2152901/range2152901/1

https://synbiohub.org/public/igem/BBa_K598001/annotation2152901/range2152901/1
type: Range
persistent identity: range2152901
display id: range2152901
version: 1
top level: BBa_K598001
direction: direction/forward
start: 910
end: 929
orientation: inline

eGFP
type: SequenceAnnotation
persistent identity: annotation2152704
display id: annotation2152704
version: 1
top level: BBa_K598001
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K598001/annotation2152704/range2152704/1

https://synbiohub.org/public/igem/BBa_K598001/annotation2152704/range2152704/1
type: Range
persistent identity: range2152704
display id: range2152704
version: 1
top level: BBa_K598001
direction: direction/forward
start: 87
end: 806
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation2152895
display id: annotation2152895
version: 1
top level: BBa_K598001
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K598001/annotation2152895/range2152895/1

https://synbiohub.org/public/igem/BBa_K598001/annotation2152895/range2152895/1
type: Range
persistent identity: range2152895
display id: range2152895
version: 1
top level: BBa_K598001
direction: direction/forward
start: 826
end: 869
orientation: inline

BBa_K259009
type: ComponentDefinition
persistent identity: BBa K259009
display id: BBa_K259009
version: 1
was generated by: iGEM to SBOL conversion
description: BioScaffold Linker - Removes Stop Codons & scars & replaces with a Gly-Ser linker
created: 2009-10-12T11:00:00Z
modified: 2015-05-08T01:11:42Z
mutable provenance: This part is completely designed ''de novo''. This sequence does not occur naturally.
owned by: james
owned by: myers
mutable description: This part is part of a family of Bioscaffold - Linkers. This part will help users interested in protein-fusion. The usage of the part will allow in frame assembly of 2(+) proteins by: *Removing the stop codons from the upstream protein. *Removing the scar created by the Assembly Standard 10 (RFC10) construction of 2(+) protein coding sequences. *Maintaining the start codon of the downstream protein. *Adding a flexible (Gly-Ser-Gly) linker between the two protein coding sequences. *Acting as a translational buffer if ligation is incorrect. ===Assembly Instructions=== *Create an Assembly Standard 10 fusion as below ; **Upstream Protein Part - Bioscaffold Linker - Downstream Protein Part** *Use the BioScaffold Specific Enzymes (i)Restrict with BpuEI to remove upstream scar (created between Upstream Protein - Bioscaffold) and stop codons from upstream protein part. (ii)Ligate and you will get the intermediate product; **Upstream Protein (w/out Stop codons) - Tyr - BioScaffold Linker - Downstream Protein** *Restrict with CspCI to remove downstream scar. *Ligate to get the final product; **Upstream Protein - Tyr-Gly-Ser-Gly- Downstream Protein** ====N.B.==== CspCI cuts with a 1 b.p discrepancy depending on the sequence. However this should not affect the restriction/ligation process as this has been taken into account and incorporated into the design. Even with the discrepancy involved the restriction/ligation process will still give the same product and in-frame fusion. The other restriction variants of CspCI have also been taken into account and relevant BioScaffolds are being produced to be tested. This version is for when CspCI cuts 13/10b.p. (top/bottom) on the 5' of the recognition sequence and 10/13bp (top/bottom) on the 3' of the…
discontinued: false
dominant: false
group u list: _357_
m user id: 0
owner id: 5318
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: The CspCI discrepancy had to be taken into account
star: false
creator: Petros Mina
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: Translational Buffer
sequence annotation: Tyr involved in restriction/ligation
sequence annotation: BpuEI
sequence annotation: BpuEI (rev. complement)
sequence annotation: Gly-Ser-Gly linker
sequence annotation: Met involved in restriction/ligation
sequence annotation: 5' CspCI Recognition sequence
sequence annotation: 3' CspCI recognition sequence
sequence: https://synbiohub.org/public/igem/BBa_K259009_sequence/1

Translational Buffer
type: SequenceAnnotation
persistent identity: annotation2037405
display id: annotation2037405
version: 1
top level: BBa_K259009
role: feature/stop
role: SO:0000319
location: https://synbiohub.org/public/igem/BBa_K259009/annotation2037405/range2037405/1

https://synbiohub.org/public/igem/BBa_K259009/annotation2037405/range2037405/1
type: Range
persistent identity: range2037405
display id: range2037405
version: 1
top level: BBa_K259009
direction: direction/forward
start: 56
end: 65
orientation: inline

Tyr involved in restriction/ligation
type: SequenceAnnotation
persistent identity: annotation2037402
display id: annotation2037402
version: 1
top level: BBa_K259009
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K259009/annotation2037402/range2037402/1

https://synbiohub.org/public/igem/BBa_K259009/annotation2037402/range2037402/1
type: Range
persistent identity: range2037402
display id: range2037402
version: 1
top level: BBa_K259009
direction: direction/forward
start: 41
end: 43
orientation: inline

BpuEI
type: SequenceAnnotation
persistent identity: annotation2037401
display id: annotation2037401
version: 1
top level: BBa_K259009
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K259009/annotation2037401/range2037401/1

https://synbiohub.org/public/igem/BBa_K259009/annotation2037401/range2037401/1
type: Range
persistent identity: range2037401
display id: range2037401
version: 1
top level: BBa_K259009
direction: direction/forward
start: 21
end: 26
orientation: inline

BpuEI (rev. complement)
type: SequenceAnnotation
persistent identity: annotation2037400
display id: annotation2037400
version: 1
top level: BBa_K259009
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K259009/annotation2037400/range2037400/1

https://synbiohub.org/public/igem/BBa_K259009/annotation2037400/range2037400/1
type: Range
persistent identity: range2037400
display id: range2037400
version: 1
top level: BBa_K259009
direction: direction/reverse
start: 3
end: 8
orientation: inline

Gly-Ser-Gly linker
type: SequenceAnnotation
persistent identity: annotation2037403
display id: annotation2037403
version: 1
top level: BBa_K259009
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K259009/annotation2037403/range2037403/1

https://synbiohub.org/public/igem/BBa_K259009/annotation2037403/range2037403/1
type: Range
persistent identity: range2037403
display id: range2037403
version: 1
top level: BBa_K259009
direction: direction/forward
start: 44
end: 52
orientation: inline

Met involved in restriction/ligation
type: SequenceAnnotation
persistent identity: annotation2037404
display id: annotation2037404
version: 1
top level: BBa_K259009
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K259009/annotation2037404/range2037404/1

https://synbiohub.org/public/igem/BBa_K259009/annotation2037404/range2037404/1
type: Range
persistent identity: range2037404
display id: range2037404
version: 1
top level: BBa_K259009
direction: direction/forward
start: 53
end: 55
orientation: inline

5' CspCI Recognition sequence
type: SequenceAnnotation
persistent identity: annotation2037437
display id: annotation2037437
version: 1
top level: BBa_K259009
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K259009/annotation2037437/range2037437/1

https://synbiohub.org/public/igem/BBa_K259009/annotation2037437/range2037437/1
type: Range
persistent identity: range2037437
display id: range2037437
version: 1
top level: BBa_K259009
direction: direction/forward
start: 66
end: 68
orientation: inline

Translational Buffer
type: SequenceAnnotation
persistent identity: annotation2037439
display id: annotation2037439
version: 1
top level: BBa_K259009
role: feature/stop
role: SO:0000319
location: https://synbiohub.org/public/igem/BBa_K259009/annotation2037439/range2037439/1

https://synbiohub.org/public/igem/BBa_K259009/annotation2037439/range2037439/1
type: Range
persistent identity: range2037439
display id: range2037439
version: 1
top level: BBa_K259009
direction: direction/forward
start: 69
end: 73
orientation: inline

3' CspCI recognition sequence
type: SequenceAnnotation
persistent identity: annotation2037438
display id: annotation2037438
version: 1
top level: BBa_K259009
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K259009/annotation2037438/range2037438/1

https://synbiohub.org/public/igem/BBa_K259009/annotation2037438/range2037438/1
type: Range
persistent identity: range2037438
display id: range2037438
version: 1
top level: BBa_K259009
direction: direction/forward
start: 74
end: 77
orientation: inline

HindIII
type: ComponentDefinition
persistent identity: BBa I11076
display id: BBa_I11076
version: 1
was generated by: iGEM to SBOL conversion
description: HindIII restriction enzyme site with extra flanking nucleotides
created: 2004-09-06T11:00:00Z
modified: 2015-08-31T04:07:30Z
mutable provenance: NEB catalog
owned by: james
owned by: myers
mutable description: HindIII restriction site with three flanking base pairs
discontinued: false
dominant: false
experience: experience/None
group u list: _2_
m user id: 0
owner id: 102
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: bu
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: HindIII
sequence: https://synbiohub.org/public/igem/BBa_I11076_sequence/1

HindIII
type: SequenceAnnotation
persistent identity: annotation1148736
display id: annotation1148736
version: 1
top level: HindIII
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I11076/annotation1148736/range1148736/1

https://synbiohub.org/public/igem/BBa_I11076/annotation1148736/range1148736/1
type: Range
persistent identity: range1148736
display id: range1148736
version: 1
top level: HindIII
direction: direction/forward
start: 4
end: 9
orientation: inline

mt ori7
type: ComponentDefinition
persistent identity: BBa K809002
display id: BBa_K809002
version: 1
was generated by: iGEM to SBOL conversion
description: ori7 region of mtDNA
created: 2012-09-19T11:00:00Z
modified: 2015-05-08T01:13:26Z
mutable provenance: NCBI database of Saccharomyces cerevisiae's genome
owned by: james
owned by: myers
mutable description: ori 7 region of Saccharomyces cerevisiae mtDNA, works as replication initial site.
discontinued: false
dominant: false
experience: experience/Works
group u list: _1066_
m user id: 0
owner id: 11943
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: none
star: false
creator: Qiwu XU
type: DnaRegion
role: partType/Other
role: SO:0000110
sequence annotation: ori7
sequence: https://synbiohub.org/public/igem/BBa_K809002_sequence/1

ori7
type: SequenceAnnotation
persistent identity: annotation2188522
display id: annotation2188522
version: 1
top level: mt ori7
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K809002/annotation2188522/range2188522/1

https://synbiohub.org/public/igem/BBa_K809002/annotation2188522/range2188522/1
type: Range
persistent identity: range2188522
display id: range2188522
version: 1
top level: mt ori7
direction: direction/forward
start: 1
end: 1
orientation: inline

ori7
type: SequenceAnnotation
persistent identity: annotation2188523
display id: annotation2188523
version: 1
top level: mt ori7
role: feature/stop
role: SO:0000319
location: https://synbiohub.org/public/igem/BBa_K809002/annotation2188523/range2188523/1

https://synbiohub.org/public/igem/BBa_K809002/annotation2188523/range2188523/1
type: Range
persistent identity: range2188523
display id: range2188523
version: 1
top level: mt ori7
direction: direction/forward
start: 378
end: 378
orientation: inline

BbvCI
type: ComponentDefinition
persistent identity: BBa M31961
display id: BBa_M31961
version: 1
was generated by: iGEM to SBOL conversion
description: BbvCI restriction enzyme site
created: 2007-02-27T12:00:00Z
modified: 2015-05-08T01:14:01Z
mutable provenance: known sequence
owned by: james
owned by: myers
mutable description: restriction site
discontinued: false
dominant: false
experience: experience/None
group u list: _102_
m user id: 0
owner id: 1381
owning group id: 102
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: none
star: false
creator: Rebecca Adams
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_M31961_sequence/1

T
type: ComponentDefinition
persistent identity: BBa G0012
display id: BBa_G0012
version: 1
was generated by: iGEM to SBOL conversion
description: The nucleotide T
created: 2007-07-22T11:00:00Z
modified: 2015-08-31T04:07:28Z
mutable provenance: None.
owned by: james
owned by: myers
mutable description: This is a part consisting of only a single nucleotide ... T. It can act as a spacer between restriction sites and parts.
discontinued: false
dominant: false
experience: experience/None
group u list: _41_
m user id: 0
owner id: 126
owning group id: 162
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: None.
star: false
creator: Reshma Shetty
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_G0012_sequence/1

Tn5R
type: ComponentDefinition
persistent identity: BBa J61022
display id: BBa_J61022
version: 1
was generated by: iGEM to SBOL conversion
description: [Tn5-rev]
created: 2006-09-20T11:00:00Z
modified: 2015-08-31T02:02:59Z
mutable provenance: Overlap extension of synthetic oligonucleotides
owned by: james
owned by: myers
mutable description: DNA substrate for Tn5 transposase. Flanking a sequence with J61021 on the 5' end and J61022 on the 3' end makes it a functional transposon. pSB1A2-Bca1013
discontinued: false
dominant: false
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: N/A
star: false
creator: John Anderson
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_J61022_sequence/1

BamHI
type: ComponentDefinition
persistent identity: BBa I11081
display id: BBa_I11081
version: 1
was generated by: iGEM to SBOL conversion
description: BamHI restriction site with extra flanking nucleotides
created: 2004-09-06T11:00:00Z
modified: 2015-08-31T04:07:30Z
mutable provenance: NEB catalogue
owned by: james
owned by: myers
mutable description: BamHI restriction site
discontinued: false
dominant: false
experience: experience/None
group u list: _2_
m user id: 0
owner id: 102
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: bu
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_I11081_sequence/1

BBa_K1330000
type: ComponentDefinition
persistent identity: BBa K1330000
display id: BBa_K1330000
version: 1
was generated by: iGEM to SBOL conversion
description: Spinach2.1 flanked by tRNALys3
created: 2014-09-23T11:00:00Z
modified: 2015-05-08T01:09:55Z
mutable provenance: Test
owned by: james
owned by: myers
mutable description: Test
discontinued: false
dominant: false
experience: experience/None
group u list: _1705_
m user id: 0
owner id: 20187
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Test
star: false
creator: Rasmus Bech
type: DnaRegion
role: partType/Measurement
role: SO:0000804
sequence: https://synbiohub.org/public/igem/BBa_K1330000_sequence/1

SX scar
type: ComponentDefinition
persistent identity: BBa G0002
display id: BBa_G0002
version: 1
was generated by: iGEM to SBOL conversion
description: SpeI/XbaI mixed site
created: 2007-02-26T12:00:00Z
modified: 2015-08-31T04:07:27Z
mutable provenance: XbaI and SpeI sites
owned by: james
owned by: myers
mutable description: XbaI/SpeI mixed site. Simply used to aid in entry of parts into the registry.
discontinued: false
dominant: true
experience: experience/None
group u list: _41_
m user id: 0
owner id: 126
owning group id: 70
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: None.
star: false
creator: Reshma Shetty
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_G0002_sequence/1

BfaI
type: ComponentDefinition
persistent identity: BBa M31967
display id: BBa_M31967
version: 1
was generated by: iGEM to SBOL conversion
description: BfaI restriction enzyme site
created: 2007-02-27T12:00:00Z
modified: 2015-05-08T01:14:01Z
mutable provenance: known sequence
owned by: james
owned by: myers
mutable description: restriction site for BfaI
discontinued: false
dominant: false
experience: experience/None
group u list: _102_
m user id: 0
owner id: 1381
owning group id: 102
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: none
star: false
creator: Rebecca Adams
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_M31967_sequence/1

amyE 3 IS
type: ComponentDefinition
persistent identity: BBa K143002
display id: BBa_K143002
version: 1
was generated by: iGEM to SBOL conversion
description: 3??? Integration Sequence for the amyE locus of B. subtilis
created: 2008-08-27T11:00:00Z
modified: 2015-05-08T01:10:23Z
mutable provenance: The 3??? integration sequence was taken from the shuttle vector pDR111 which has been used in many studies on B.subtilis, in particular in the studies of transcriptional control[1,2,3] References 1.Shimotsu H and Henner DJ. Construction of a single-copy integration vector and its use in analysis of regulation of the trp operon of Bacillus subtilis. Gene 1986; 43(1-2) 85-94. pmid:3019840. 2.Erwin KN, Nakano S, and Zuber P. Sulfate-dependent repression of genes that function in organosulfur metabolism in Bacillus subtilis requires Spx. J Bacteriol 2005 Jun; 187(12) 4042-9. doi:10.1128/JB.187.12.4042-4049.2005 pmid:15937167 3.Britton RA, Eichenberger P, Gonzalez-Pastor JE, Fawcett P, Monson R, Losick R, and Grossman AD. Genome-wide analysis of the stationary-phase sigma factor (sigma-H) regulon of Bacillus subtilis. J Bacteriol 2002 Sep; 184(17) 4881-90. pmid:12169614
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Integration sequences allow DNA to be incorporated into the chromosome of a host cell at a specific locus using leading (5') and trailing (3') DNA sequences that are the same as those at a specific locus of the chromosome. The 5' integration sequence can be added to the front of a Biobrick construct and the 3' integration sequence specific for this locus (Part BBa_K143002) to the rear of the Biobrick construct to allow integration of the Biobrick construct into the chromosome of the gram positive bacterium B.subtilis. The AmyE locus was the first locus used for integration into B.subtilis by Shimotsu and Henner[1] and is still commonly used in vectors such as pDR111[2], pDL[3] and their derivatives. Integration at the AmyE locus removes the ability of B.subtilis to break down starch, which can be assayed with iodine as described by Cutting and Vander-horn[4]. The 5' and 3' integration sequences for the AmyE locus were used to integrate the Imperial 2008 iGEM project primary construct into the B.sutbilis chromosome. References 1. Shimotsu H and Henner DJ. Construction of a single-copy integration vector and its use in analysis of regulation of the trp operon of Bacillus subtilis. Gene 1986; 43(1-2) 85-94. pmid:3019840 2.Nakano S, Kster-Schck E, Grossman AD, and Zuber P. Spx-dependent global transcriptional control is induced by thiol-specific oxidative stress in Bacillus subtilis. Proc Natl Acad Sci U S A 2003 Nov 11; 100(23) 13603-8. doi:10.1073/pnas.2235180100 pmid:14597697 3.Bacillus Genetic Stock Center; www.bgsc.org 4.Cutting, S M.; Vander-Horn, P B. Genetic analysis. In: Harwood C R, Cutting S M. , editors. Molecular biological methods for Bacillus. Chichester, England: John Wiley & Sons, Ltd.; 1990. pp. 27???74.
discontinued: false
dominant: false
experience: experience/Works
group u list: _199_
m user id: 0
owner id: 3475
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable notes: The AmyE integration sequence was taken from the vector after comparison by BLAST to the B.subtilis chromosome to identify the homologous sequences. The sequence present in both the host chromosome and the plasmid at the 3' end of the gene is the 3' sequence required for integration
star: true
creator: Chris Hirst
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: 3' AmyE homologous sequence
sequence: https://synbiohub.org/public/igem/BBa_K143002_sequence/1

3' AmyE homologous sequence
type: SequenceAnnotation
persistent identity: annotation1974146
display id: annotation1974146
version: 1
top level: amyE 3 IS
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K143002/annotation1974146/range1974146/1

https://synbiohub.org/public/igem/BBa_K143002/annotation1974146/range1974146/1
type: Range
persistent identity: range1974146
display id: range1974146
version: 1
top level: amyE 3 IS
direction: direction/forward
start: 1
end: 1005
orientation: inline

dumbbell
type: ComponentDefinition
persistent identity: BBa J35002
display id: BBa_J35002
version: 1
was generated by: iGEM to SBOL conversion
description: dumbbell hairpin
created: 2006-10-19T11:00:00Z
modified: 2015-08-31T04:08:47Z
mutable provenance: artificial sequence
owned by: james
owned by: myers
mutable description: DNA origami
discontinued: false
dominant: false
experience: experience/None
group u list: _64_
m user id: 0
owner id: 807
owning group id: 64
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable notes: OligoAnalyzer 3.0 http://www.idtdna.com/analyzer/Applications/OligoAnalyzer/
star: false
creator: Andrey Kuznetsov
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_J35002_sequence/1

BBa_M1001
type: ComponentDefinition
persistent identity: BBa M1001
display id: BBa_M1001
version: 1
was generated by: iGEM to SBOL conversion
description: Right mosaic end for Tn5 transpososome
creator: Tom Knight
created: 2006-03-16T12:00:00Z
modified: 2015-05-08T01:13:51Z
discontinued: false
dominant: false
experience: experience/None
group u list: _12_41_48_1_
m user id: 0
owner id: 6
owning group id: 48
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Right end of a linear transpososome sequence containing the 19 bp mosaic end in the reverse orientation. The sequence also contains a PvuII cut site which releases the transpososome when cut with PvuII. Constructed compound parts intended for transposition must not contain other PvuII end, or be PCR amplified with blunt end producing enzymes (Pfu, Phusion) at the PvuII cut site between CAG and CTG. This part, and the companion part M1000 provide Biobrick tools to construct Tn5 transpososomes: linear DNA sequences which can be cut in vitro from a plasmid backbone, or created by PCR, combined in vitro with purified Tn5 trasposase protein, electroportated into cells, and finally randomly inserting into the bacterial chromosome. The parts assembled between the two mosaic ends can be delivered, including antibiotic resistance markers or other functional chromosomal insertions.
mutable notes: ;biobrick left end, TA cloning overhang GTT TCT TCG AAT TCG CGG CCG CTT CTA GAG ;biobrick right end, TA cloning overhang GTT TCT TCC TGC AGC GGC CGC TAC TAG TA ;mosaic end with cag upstream to insert PvuII cut site cagCTGTCTCTTATACACATCT ;mosaic end reverse complement with ctg upstream to insert PvuII cut site AGATGTGTATAAGAGACAGctg ;mosaic left end in biobrick form construction primer F GTT TCT TCG AAT TCG CGG CCG CTT CTA GAG cagCTGTCTCTTATACACATCT, MEL-F ;mosaic left end in biobrick form construction primer R GTT TCT TCC TGC AGC GGC CGC TAC TAG TA AGATGTGTATAAGAGACAGctg, MEL-R ;mosaic right end in biobrick form construction primer F GTT TCT TCG AAT TCG CGG CCG CTT CTA GAG AGATGTGTATAAGAGACAGctg, MER-F ;mosaic right end in biobrick form construction primer R GTT TCT TCC TGC AGC GGC CGC TAC TAG TA cagCTGTCTCTTATACACATCT, MER-R
mutable provenance: Synthesis from overlapped oligos with PCR.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: Mosaic end (inverted)
sequence annotation: PvuII cut site
sequence: https://synbiohub.org/public/igem/BBa_M1001_sequence/1

Mosaic end (inverted)
type: SequenceAnnotation
persistent identity: annotation1825561
display id: annotation1825561
version: 1
top level: BBa_M1001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_M1001/annotation1825561/range1825561/1

https://synbiohub.org/public/igem/BBa_M1001/annotation1825561/range1825561/1
type: Range
persistent identity: range1825561
display id: range1825561
version: 1
direction: direction/forward
top level: BBa_M1001
start: 1
end: 19
orientation: inline

PvuII cut site
type: SequenceAnnotation
persistent identity: annotation1825560
display id: annotation1825560
version: 1
top level: BBa_M1001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_M1001/annotation1825560/range1825560/1

https://synbiohub.org/public/igem/BBa_M1001/annotation1825560/range1825560/1
type: Range
persistent identity: range1825560
display id: range1825560
version: 1
direction: direction/forward
top level: BBa_M1001
start: 17
end: 22
orientation: inline

BBa_I13452
type: ComponentDefinition
persistent identity: BBa I13452
display id: BBa_I13452
version: 1
was generated by: iGEM to SBOL conversion
description: Adds another BioBrick site
created: 2005-05-24T11:00:00Z
modified: 2015-08-31T04:07:34Z
owned by: myers
owned by: james
part status: Released HQ 2013
mutable description: Contains both a BbsI and a BstXI restriction site. When cut with both enzymes, it leaves an EcoRI and a PstI site.
discontinued: false
dominant: false
experience: experience/None
group u list: _11_
m user id: 0
owner id: 253
owning group id: 6
sample status: In stock
status: status/Available
bookmark: false
mutable notes: 70 bp from Eco to Spe or Xba to Pst, to allow for gel extraction. The middle portion is largely noise, intended as filler to raise the size of the part.
star: true
creator: jkm
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: BbsI site
sequence annotation: Future Pst site
sequence annotation: Future Eco site
sequence annotation: BstXI site
sequence: https://synbiohub.org/public/igem/BBa_I13452_sequence/1

BbsI site
type: SequenceAnnotation
persistent identity: annotation1891620
display id: annotation1891620
version: 1
top level: BBa_I13452
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I13452/annotation1891620/range1891620/1

https://synbiohub.org/public/igem/BBa_I13452/annotation1891620/range1891620/1
type: Range
persistent identity: range1891620
display id: range1891620
version: 1
top level: BBa_I13452
direction: direction/forward
start: 8
end: 13
orientation: inline

Future Pst site
type: SequenceAnnotation
persistent identity: annotation1891622
display id: annotation1891622
version: 1
top level: BBa_I13452
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I13452/annotation1891622/range1891622/1

https://synbiohub.org/public/igem/BBa_I13452/annotation1891622/range1891622/1
type: Range
persistent identity: range1891622
display id: range1891622
version: 1
top level: BBa_I13452
direction: direction/forward
start: 44
end: 49
orientation: inline

Future Eco site
type: SequenceAnnotation
persistent identity: annotation1891619
display id: annotation1891619
version: 1
top level: BBa_I13452
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I13452/annotation1891619/range1891619/1

https://synbiohub.org/public/igem/BBa_I13452/annotation1891619/range1891619/1
type: Range
persistent identity: range1891619
display id: range1891619
version: 1
top level: BBa_I13452
direction: direction/forward
start: 1
end: 6
orientation: inline

BstXI site
type: SequenceAnnotation
persistent identity: annotation1891621
display id: annotation1891621
version: 1
top level: BBa_I13452
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I13452/annotation1891621/range1891621/1

https://synbiohub.org/public/igem/BBa_I13452/annotation1891621/range1891621/1
type: Range
persistent identity: range1891621
display id: range1891621
version: 1
top level: BBa_I13452
direction: direction/forward
start: 41
end: 52
orientation: inline

BciVI
type: ComponentDefinition
persistent identity: BBa M31964
display id: BBa_M31964
version: 1
was generated by: iGEM to SBOL conversion
description: BciVI restriction enzyme site
created: 2007-02-27T12:00:00Z
modified: 2015-05-08T01:14:01Z
mutable provenance: known sequence
owned by: james
owned by: myers
mutable description: restriction site
discontinued: false
dominant: false
experience: experience/None
group u list: _102_
m user id: 0
owner id: 1381
owning group id: 102
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: none
star: false
creator: Rebecca Adams
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_M31964_sequence/1

EagI
type: ComponentDefinition
persistent identity: BBa M31986
display id: BBa_M31986
version: 1
was generated by: iGEM to SBOL conversion
description: EagI restriction enzyme site
created: 2007-02-28T12:00:00Z
modified: 2015-05-08T01:14:01Z
mutable provenance: none
owned by: james
owned by: myers
mutable description: EagI restriction enzyme site
discontinued: false
dominant: false
experience: experience/None
group u list: _102_
m user id: 0
owner id: 1373
owning group id: 102
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: none
star: false
creator: Tiffany Guo
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_M31986_sequence/1

SapI (reve
type: ComponentDefinition
persistent identity: BBa B0103
display id: BBa_B0103
version: 1
was generated by: iGEM to SBOL conversion
description: SapI restriction enzyme site, reversed (offset cutter)
created: 2007-03-05T12:00:00Z
modified: 2015-08-31T04:07:21Z
mutable provenance: NEB catalog.
owned by: james
owned by: myers
mutable description: SapI restriction site in the reverse orientation (recognition sequence on the 3' strand). SapI is an offsite cutter. From left to right this part includes the designed 3 bp 5' overhang (ATG), a single bp spacer, the 7 bp recognition sequence (5'-GCTCTTCT-3'), and two bp spacer. Cleavage at this site results in a 3 bp, 5' overhang (left side, top strand), which makes it compatible with Heather Keller's RBS assembly scheme for creating RBS/CDS junctions without a mixed site. This part is specific for CDSs with an ATG start site and is not compatible with GTG start sites (via HKs assembly scheme). The single bp spacer on the 3' end allows RBSes construct with this part to be used in standard biobricks assembly and is necessary to maintain the reading frame of a downstream CDS. Note that Standard Biobricks Assembly with this site results in the creation of a new ATG start site, and a 7 amino acid fusion at the N terminus of the downstream protein.
discontinued: false
dominant: false
experience: experience/None
group u list: _11_
m user id: 0
owner id: 571
owning group id: 10
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: This part only exists in composite parts within the registry. This restriction site is used by Heather Keller for creating blunt junctions between RBS sequences and coding regions (i.e. no biobricks scar in between).
star: false
creator: Heather Keller
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: 5' overhang
sequence annotation: SapI
sequence: https://synbiohub.org/public/igem/BBa_B0103_sequence/1

5' overhang
type: SequenceAnnotation
persistent identity: annotation1920081
display id: annotation1920081
version: 1
top level: SapI (reve
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_B0103/annotation1920081/range1920081/1

https://synbiohub.org/public/igem/BBa_B0103/annotation1920081/range1920081/1
type: Range
persistent identity: range1920081
display id: range1920081
version: 1
top level: SapI (reve
direction: direction/forward
start: 1
end: 3
orientation: inline

SapI
type: SequenceAnnotation
persistent identity: annotation1920082
display id: annotation1920082
version: 1
top level: SapI (reve
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_B0103/annotation1920082/range1920082/1

https://synbiohub.org/public/igem/BBa_B0103/annotation1920082/range1920082/1
type: Range
persistent identity: range1920082
display id: range1920082
version: 1
top level: SapI (reve
direction: direction/reverse
start: 5
end: 11
orientation: inline

BBa_K2027049
type: ComponentDefinition
persistent identity: BBa K2027049
display id: BBa_K2027049
version: 1
was generated by: iGEM to SBOL conversion
description: PQQ Aptamer #1 (15ADa9)
created: 2016-10-23T11:00:00Z
modified: 2016-10-27T10:45:34Z
mutable provenance: Emahi et al. 2014 (http://www.rsc.org/suppdata/ra/c4/c4ra11052h/c4ra11052h1.pdf)
owned by: james
owned by: myers
mutable description: This is a non-coding RNA that encodes the pyrroloquinoline quinone binding aptamer. It can be used in both binding and sensing applications.
discontinued: false
dominant: false
experience: experience/None
group u list: _2494_
m user id: 29123
owner id: 29123
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: This was one of the best two DNA aptamer candidates from SELEX (also see BBa_K2027049).
star: false
creator: Michael Becich
type: DnaRegion
role: partType/Other
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_K2027049_sequence/1

BBa_K106016
type: ComponentDefinition
persistent identity: BBa K106016
display id: BBa_K106016
version: 1
was generated by: iGEM to SBOL conversion
description: AarI "B" site scar sequence
created: 2008-10-23T11:00:00Z
modified: 2015-05-08T01:08:58Z
mutable provenance: For registry assembly only.
owned by: james
owned by: myers
mutable description: For registry assembly only.
discontinued: false
dominant: false
experience: experience/None
group u list: _226_
m user id: 0
owner id: 2659
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: For registry assembly only.
star: false
creator: Andrew Horwitz
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_K106016_sequence/1

A scar
type: ComponentDefinition
persistent identity: BBa K106015
display id: BBa_K106015
version: 1
was generated by: iGEM to SBOL conversion
description: AarI "A" site scar sequence
created: 2008-10-23T11:00:00Z
modified: 2015-05-08T01:08:58Z
mutable provenance: For registry assembly only.
owned by: james
owned by: myers
mutable description: Scar site created by A part ligation. Used for entering composite parts on the Registry.
discontinued: false
dominant: false
experience: experience/None
group u list: _226_
m user id: 0
owner id: 2659
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: For registry assembly only.
star: false
creator: Andrew Horwitz
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_K106015_sequence/1

BamHI
type: ComponentDefinition
persistent identity: BBa K150008
display id: BBa_K150008
version: 1
was generated by: iGEM to SBOL conversion
description: BamHI restriction enzyme site
created: 2008-10-20T11:00:00Z
modified: 2015-05-08T01:10:46Z
mutable provenance: -
owned by: james
owned by: myers
mutable description: BamHI restriction site
discontinued: false
dominant: true
experience: experience/None
group u list: _234_
m user id: 0
owner id: 3299
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: -
star: false
creator: Pascal Kraemer
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_K150008_sequence/1

Suffix
type: ComponentDefinition
persistent identity: BBa G00001
display id: BBa_G00001
version: 1
was generated by: iGEM to SBOL conversion
description: BioBrick cloning site suffix
created: 2006-03-13T12:00:00Z
modified: 2015-08-31T04:07:27Z
owned by: james
owned by: myers
mutable description: This part is the standard BioBricks suffix.
discontinued: false
dominant: true
experience: experience/Works
group u list: _41_6_
m user id: 0
owner id: 126
owning group id: 45
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Reshma Shetty
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: PstI
sequence annotation: NotI
sequence annotation: Suffix
sequence annotation: SpeI
sequence: https://synbiohub.org/public/igem/BBa_G00001_sequence/1

PstI
type: SequenceAnnotation
persistent identity: annotation1934227
display id: annotation1934227
version: 1
top level: Suffix
role: feature/dna
role: SO:0000110
location: https://synbiohub.org/public/igem/BBa_G00001/annotation1934227/range1934227/1

https://synbiohub.org/public/igem/BBa_G00001/annotation1934227/range1934227/1
type: Range
persistent identity: range1934227
display id: range1934227
version: 1
top level: Suffix
direction: direction/forward
start: 16
end: 21
orientation: inline

NotI
type: SequenceAnnotation
persistent identity: annotation1934228
display id: annotation1934228
version: 1
top level: Suffix
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_G00001/annotation1934228/range1934228/1

https://synbiohub.org/public/igem/BBa_G00001/annotation1934228/range1934228/1
type: Range
persistent identity: range1934228
display id: range1934228
version: 1
top level: Suffix
direction: direction/forward
start: 9
end: 16
orientation: inline

Suffix
type: SequenceAnnotation
persistent identity: annotation1821544
display id: annotation1821544
version: 1
top level: Suffix
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_G00001/annotation1821544/range1821544/1

https://synbiohub.org/public/igem/BBa_G00001/annotation1821544/range1821544/1
type: Range
persistent identity: range1821544
display id: range1821544
version: 1
top level: Suffix
direction: direction/forward
start: 1
end: 21
orientation: inline

SpeI
type: SequenceAnnotation
persistent identity: annotation1934226
display id: annotation1934226
version: 1
top level: Suffix
role: feature/dna
role: SO:0000110
location: https://synbiohub.org/public/igem/BBa_G00001/annotation1934226/range1934226/1

https://synbiohub.org/public/igem/BBa_G00001/annotation1934226/range1934226/1
type: Range
persistent identity: range1934226
display id: range1934226
version: 1
top level: Suffix
direction: direction/forward
start: 2
end: 7
orientation: inline

XbaI
type: ComponentDefinition
persistent identity: BBa M31991
display id: BBa_M31991
version: 1
was generated by: iGEM to SBOL conversion
description: XbaI restriction enzyme site
created: 2007-02-28T12:00:00Z
modified: 2015-05-08T01:14:01Z
mutable provenance: none
owned by: james
owned by: myers
mutable description: XbaI restriction enzyme site
discontinued: false
dominant: false
experience: experience/None
group u list: _102_
m user id: 0
owner id: 1373
owning group id: 102
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: none
star: false
creator: Tiffany Guo
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_M31991_sequence/1

Tetrahedro
type: ComponentDefinition
persistent identity: BBa K2054000
display id: BBa_K2054000
version: 1
was generated by: iGEM to SBOL conversion
description: All Oligos for Tetrahedral Nanostructure
creator: LAI HEI WAI
created: 2016-10-13T11:00:00Z
modified: 2016-10-27T11:55:05Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2522_
m user id: 0
m user id: 30316
owner id: 30316
owning group id: 9
sample status: Not in stock
status: status/Planning
status: status/Unavailable
bookmark: false
mutable description: This will be used to build and express our desired functional nanostructure in E. coli that target micro RNA biomarkers. This plasmid encodes all the ssDNA that will make the tetrahedral nanostructure, i.e. Oligos 1, 2, 3, 4 and 5.
mutable notes: The assembly of the structure.
mutable provenance: Elbaz, J., Yin, P., & Voigt, C. A. (2016). Genetic encoding of DNA nanostructures and their self-assembly in living bacteria. Nature communications, 7.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_K2054000_sequence/1

BclI
type: ComponentDefinition
persistent identity: BBa M31987
display id: BBa_M31987
version: 1
was generated by: iGEM to SBOL conversion
description: BclI restriction enzyme site
created: 2007-02-28T12:00:00Z
modified: 2015-05-08T01:14:01Z
mutable provenance: none
owned by: james
owned by: myers
mutable description: BclI restriction enzyme site
discontinued: false
dominant: false
experience: experience/None
group u list: _102_
m user id: 0
owner id: 1373
owning group id: 102
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: none
star: false
creator: Tiffany Guo
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_M31987_sequence/1

VR
type: ComponentDefinition
persistent identity: BBa G00102
display id: BBa_G00102
version: 1
was generated by: iGEM to SBOL conversion
description: Reverse BioBrick primer annealing site (VR binding site)
created: 2006-02-03T12:00:00Z
modified: 2015-08-31T04:07:28Z
owned by: james
owned by: myers
mutable description: This is the VR sequence to be used in construction of plasmids which include the VR primer site. It is the reverse complement of BBa_G00101.
discontinued: false
dominant: false
experience: experience/Works
group u list: _41_6_
m user id: 0
owner id: 126
owning group id: 45
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Reshma Shetty
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: VR
sequence: https://synbiohub.org/public/igem/BBa_G00102_sequence/1

VR
type: SequenceAnnotation
persistent identity: annotation1961229
display id: annotation1961229
version: 1
top level: VR
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_G00102/annotation1961229/range1961229/1

https://synbiohub.org/public/igem/BBa_G00102/annotation1961229/range1961229/1
type: Range
persistent identity: range1961229
display id: range1961229
version: 1
top level: VR
direction: direction/reverse
start: 1
end: 20
orientation: inline

O5
type: ComponentDefinition
persistent identity: BBa K2054005
display id: BBa_K2054005
version: 1
was generated by: iGEM to SBOL conversion
description: Oligo 5 of DNA Tetrahedral Nanostructure
creator: LAI HEI WAI
created: 2016-10-13T11:00:00Z
modified: 2016-10-27T11:57:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2522_
m user id: 0
m user id: 30316
owner id: 30316
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This will be used to build our desired nanostructure that target micro RNA biomarkers. This plasmid encodes the second ssDNA that will make the tetrahedral nanostructure, together with oligo 1, 2, 3 and 4.
mutable notes: The assembly of the structure.
mutable provenance: Elbaz, J., Yin, P., & Voigt, C. A. (2016). Genetic encoding of DNA nanostructures and their self-assembly in living bacteria. Nature communications, 7.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_K2054005_sequence/1

XmaI
type: ComponentDefinition
persistent identity: BBa I11074
display id: BBa_I11074
version: 1
was generated by: iGEM to SBOL conversion
description: XmaI restriction enzyme site with extra flanking nucleotides
created: 2004-09-06T11:00:00Z
modified: 2015-08-31T04:07:30Z
mutable provenance: NEB catalog
owned by: james
owned by: myers
mutable description: XmaI restriction site with 3 flanking base pairs
discontinued: false
dominant: false
experience: experience/None
group u list: _2_
m user id: 0
owner id: 102
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: bu
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: XmaI
sequence: https://synbiohub.org/public/igem/BBa_I11074_sequence/1

XmaI
type: SequenceAnnotation
persistent identity: annotation1148485
display id: annotation1148485
version: 1
top level: XmaI
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I11074/annotation1148485/range1148485/1

https://synbiohub.org/public/igem/BBa_I11074/annotation1148485/range1148485/1
type: Range
persistent identity: range1148485
display id: range1148485
version: 1
top level: XmaI
direction: direction/forward
start: 4
end: 9
orientation: inline

Scar25 TAA
type: ComponentDefinition
persistent identity: BBa K1319107
display id: BBa_K1319107
version: 1
was generated by: iGEM to SBOL conversion
description: RFC 25 Translation Stop Scar Sequence
created: 2014-10-02T11:00:00Z
modified: 2015-05-08T01:09:51Z
mutable provenance: This sequence is produced by an assembly method.
owned by: james
owned by: myers
mutable description: This sequence is produced by RFC10-assembly of a RFC25-CDS with a RFC10-prefixed part.
discontinued: false
dominant: false
experience: experience/None
group u list: _1694_
m user id: 0
owner id: 19522
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: The resulting sequence is a combination of 9 nucleotides from the RFC25 prefix (acc ggt taa) and the RFC10 scar (tactagag).
star: false
creator: Michael Osthege
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_K1319107_sequence/1

stop
type: SequenceAnnotation
persistent identity: annotation2393318
display id: annotation2393318
version: 1
top level: Scar25 TAA
role: feature/stop
role: SO:0000319
location: https://synbiohub.org/public/igem/BBa_K1319107/annotation2393318/range2393318/1

https://synbiohub.org/public/igem/BBa_K1319107/annotation2393318/range2393318/1
type: Range
persistent identity: range2393318
display id: range2393318
version: 1
top level: Scar25 TAA
direction: direction/forward
start: 7
end: 9
orientation: inline

Tn5F
type: ComponentDefinition
persistent identity: BBa J61021
display id: BBa_J61021
version: 1
was generated by: iGEM to SBOL conversion
description: [Tn5-for]
created: 2006-09-20T11:00:00Z
modified: 2015-08-31T02:02:59Z
mutable provenance: Overlap extension of synthetic oligonucleotides
owned by: james
owned by: myers
mutable description: DNA substrate for Tn5 transposase. Flanking a sequence with J61021 on the 5' end and J61022 on the 3' end makes it a functional transposon.
discontinued: false
dominant: false
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: N/A
star: false
creator: John Anderson
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_J61021_sequence/1

BBa_J70032
type: ComponentDefinition
persistent identity: BBa J70032
display id: BBa_J70032
version: 1
was generated by: iGEM to SBOL conversion
description: A Composite BioScaffold Part (PpiI and PsrI) for Protein Fusions (see Design Page)
created: 2008-12-12T12:00:00Z
modified: 2015-05-08T01:08:20Z
mutable provenance: Designed
owned by: myers
owned by: james
mutable description: Brings together a BioScaffold part that removes protein tails with one that removes protein heads in order to create a BioScaffold part that can help create protein fusions using standard assembly
discontinued: false
dominant: false
experience: experience/None
group u list: _41_
m user id: 0
owner id: 1201
owning group id: 41
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: The part that links the two BioScaffold parts can be altered as desired--here I just wanted to leave at least 7 bp beyond the internal scars.
star: false
creator: Julie Norville
type: DnaRegion
role: partType/DNA
role: SO:0000110
component: BBa_J70031
component: BBa_J70012
component: BBa_J70030
sequence annotation: BBa_J70031
sequence annotation: BBa_J70030
sequence annotation: BBa_J70012
sequence: https://synbiohub.org/public/igem/BBa_J70032_sequence/1

BBa_J70031
type: Component
persistent identity: component2000357
display id: component2000357
version: 1
top level: BBa_J70032
access: public
definition: BBa_J70031

BBa_J70012
type: Component
persistent identity: component2000365
display id: component2000365
version: 1
top level: BBa_J70032
access: public
definition: BBa_J70012

BBa_J70030
type: Component
persistent identity: component2000353
display id: component2000353
version: 1
top level: BBa_J70032
access: public
definition: BBa_J70030

BBa_J70031
type: SequenceAnnotation
persistent identity: annotation2000357
display id: annotation2000357
version: 1
top level: BBa_J70032
location: https://synbiohub.org/public/igem/BBa_J70032/annotation2000357/range2000357/1
component: BBa_J70031

https://synbiohub.org/public/igem/BBa_J70032/annotation2000357/range2000357/1
type: Range
persistent identity: range2000357
display id: range2000357
version: 1
top level: BBa_J70032
direction: direction/reverse
start: 57
end: 78
orientation: inline

BBa_J70030
type: SequenceAnnotation
persistent identity: annotation2000353
display id: annotation2000353
version: 1
top level: BBa_J70032
location: https://synbiohub.org/public/igem/BBa_J70032/annotation2000353/range2000353/1
component: BBa_J70030

https://synbiohub.org/public/igem/BBa_J70032/annotation2000353/range2000353/1
type: Range
persistent identity: range2000353
display id: range2000353
version: 1
top level: BBa_J70032
direction: direction/reverse
start: 1
end: 48
orientation: inline

BBa_J70012
type: SequenceAnnotation
persistent identity: annotation2000365
display id: annotation2000365
version: 1
top level: BBa_J70032
location: https://synbiohub.org/public/igem/BBa_J70032/annotation2000365/range2000365/1
component: BBa_J70012

https://synbiohub.org/public/igem/BBa_J70032/annotation2000365/range2000365/1
type: Range
persistent identity: range2000365
display id: range2000365
version: 1
top level: BBa_J70032
direction: direction/reverse
start: 87
end: 138
orientation: inline

BBa_K175000
type: ComponentDefinition
persistent identity: BBa K175000
display id: BBa_K175000
version: 1
was generated by: iGEM to SBOL conversion
description: trbC (pilin) of IncP beta R751 plasmid
created: 2009-07-04T11:00:00Z
modified: 2015-05-08T01:10:58Z
mutable provenance: This part comes from http://www.ncbi.nlm.nih.gov/nuccore/70907607?from=48797&to=51040&report=gbwithparts and corresponds to range 48797..51040 of the R751 sequence.
owned by: james
owned by: myers
mutable description: The relaxase / helicase TraI of the IncP beta plasmid R751. Essential of conjugative transfer. This part nicks oriT. It should be placed on a message plasmid along with oriT, and will enable transfer of the message plasmid in the presence of a modified R751 helper plasmid such as BBa_I714037.
discontinued: false
dominant: false
group u list: _280_
m user id: 0
owner id: 4286
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: None
star: false
creator: Calin Plesa
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: trbC
sequence: https://synbiohub.org/public/igem/BBa_K175000_sequence/1

trbC
type: SequenceAnnotation
persistent identity: annotation2008011
display id: annotation2008011
version: 1
top level: BBa_K175000
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K175000/annotation2008011/range2008011/1

https://synbiohub.org/public/igem/BBa_K175000/annotation2008011/range2008011/1
type: Range
persistent identity: range2008011
display id: range2008011
version: 1
top level: BBa_K175000
direction: direction/forward
start: 1
end: 468
orientation: inline

scar
type: ComponentDefinition
persistent identity: BBa G0003
display id: BBa_G0003
version: 1
was generated by: iGEM to SBOL conversion
description: NheI/XbaI mixed site
created: 2007-02-26T12:00:00Z
modified: 2015-08-31T04:07:27Z
mutable provenance: NheI and XbaI
owned by: james
owned by: myers
mutable description: NheI/XbaI mixed site
discontinued: false
dominant: false
experience: experience/None
group u list: _41_
m user id: 0
owner id: 126
owning group id: 70
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: None.
star: false
creator: Reshma Shetty
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_G0003_sequence/1

BBa_J70052
type: ComponentDefinition
persistent identity: BBa J70052
display id: BBa_J70052
version: 1
was generated by: iGEM to SBOL conversion
description: Right Library Insert Preparation Part (To use with J70050, and J70040)
created: 2009-01-22T12:00:00Z
modified: 2015-05-08T01:08:20Z
mutable provenance: Designed
owned by: james
owned by: myers
mutable description: Still in Testing Surround a part with J70050 and J70052, digest with AarI Insert into a vector that contained J70040 and had J70040 removed by AarI and MabI
discontinued: false
dominant: false
experience: experience/None
group u list: _41_
m user id: 0
owner id: 1201
owning group id: 41
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Not sure if one AarI site on each site will be enough.
star: false
creator: Julie Norville
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: AarI
sequence annotation: Spacer
sequence: https://synbiohub.org/public/igem/BBa_J70052_sequence/1

AarI
type: SequenceAnnotation
persistent identity: annotation2000147
display id: annotation2000147
version: 1
top level: BBa_J70052
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J70052/annotation2000147/range2000147/1

https://synbiohub.org/public/igem/BBa_J70052/annotation2000147/range2000147/1
type: Range
persistent identity: range2000147
display id: range2000147
version: 1
top level: BBa_J70052
direction: direction/forward
start: 4
end: 10
orientation: inline

Spacer
type: SequenceAnnotation
persistent identity: annotation2000146
display id: annotation2000146
version: 1
top level: BBa_J70052
role: feature/dna
role: SO:0000110
location: https://synbiohub.org/public/igem/BBa_J70052/annotation2000146/range2000146/1

https://synbiohub.org/public/igem/BBa_J70052/annotation2000146/range2000146/1
type: Range
persistent identity: range2000146
display id: range2000146
version: 1
top level: BBa_J70052
direction: direction/forward
start: 1
end: 3
orientation: inline

BBa_J35000
type: ComponentDefinition
persistent identity: BBa J35000
display id: BBa_J35000
version: 1
was generated by: iGEM to SBOL conversion
description: Rothemund DNA origami part
created: 2006-10-17T11:00:00Z
modified: 2015-08-31T04:08:47Z
mutable provenance: Artificial sequence.
owned by: james
owned by: myers
mutable description: We use this sequence to insert into staples as a level 1 pixel.
discontinued: false
dominant: false
experience: experience/Works
group u list: _64_
m user id: 0
owner id: 807
owning group id: 64
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Rothemund PW. Folding DNA to create nanoscale shapes and patterns. Nature. 2006 Mar 16;440(7082):297-302.
star: false
creator: Andrey Kuznetsov
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_J35000_sequence/1

3-fingers
type: ComponentDefinition
persistent identity: BBa J35003
display id: BBa_J35003
version: 1
was generated by: iGEM to SBOL conversion
description: 3-fingers arm
created: 2006-10-22T11:00:00Z
modified: 2015-08-31T04:08:47Z
mutable provenance: artificial sequence
owned by: james
owned by: myers
mutable description: DNA ORIGAMI BioBrick
discontinued: false
dominant: false
experience: experience/None
group u list: _64_
m user id: 0
owner id: 807
owning group id: 64
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: OligoAnalyzer 3.0 http://www.idtdna.com/analyzer/Applications/OligoAnalyzer/
star: false
creator: Andrey Kuznetsov
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_J35003_sequence/1

BBa_J36856
type: ComponentDefinition
persistent identity: BBa J36856
display id: BBa_J36856
version: 1
was generated by: iGEM to SBOL conversion
description: S50 linkaptamer
created: 2006-10-28T11:00:00Z
modified: 2015-08-31T04:08:47Z
mutable provenance: synthetic
owned by: james
owned by: myers
mutable description: S50 is the streptavidin-binding component of the A50 adaptamer which can physically link together the proteins thrombin and streptavidin. A50 contains a 45 bp double-stranded linking region between thrombin and streptavidin aptamer sequences.
discontinued: false
dominant: false
experience: experience/None
group u list: _65_
m user id: 0
owner id: 1245
owning group id: 65
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Linking region had to avoid disruption of aptamer secondary structure.
star: false
creator: Lewis Hahn
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_J36856_sequence/1

Down
type: ComponentDefinition
persistent identity: BBa K1642006
display id: BBa_K1642006
version: 1
was generated by: iGEM to SBOL conversion
description: Downstream sequence from slr0168
created: 2015-08-14T11:00:00Z
modified: 2015-09-16T01:32:30Z
mutable provenance: Slr
owned by: james
owned by: myers
mutable description: Slr
discontinued: false
dominant: false
experience: experience/None
group u list: _2059_
m user id: 24423
owner id: 24405
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: Slr
star: false
creator: Can Yin
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_K1642006_sequence/1

Adapter
type: ComponentDefinition
persistent identity: BBa I732007
display id: BBa_I732007
version: 1
was generated by: iGEM to SBOL conversion
description: BglII + BamHI
created: 2007-07-06T11:00:00Z
modified: 2015-08-31T04:07:56Z
mutable provenance: http://openwetware.org/wiki/Silver:_Oligonucleotide_Inserts Oligonucleotide Inserts
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: BglII and BamHI restriction sites with some flanking
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: None
star: true
creator: Zhan Jian
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: BglII
sequence annotation: BamHI
sequence: https://synbiohub.org/public/igem/BBa_I732007_sequence/1

BglII
type: SequenceAnnotation
persistent identity: annotation1936907
display id: annotation1936907
version: 1
top level: Adapter
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I732007/annotation1936907/range1936907/1

https://synbiohub.org/public/igem/BBa_I732007/annotation1936907/range1936907/1
type: Range
persistent identity: range1936907
display id: range1936907
version: 1
top level: Adapter
direction: direction/forward
start: 3
end: 8
orientation: inline

BamHI
type: SequenceAnnotation
persistent identity: annotation1936908
display id: annotation1936908
version: 1
top level: Adapter
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I732007/annotation1936908/range1936908/1

https://synbiohub.org/public/igem/BBa_I732007/annotation1936908/range1936908/1
type: Range
persistent identity: range1936908
display id: range1936908
version: 1
top level: Adapter
direction: direction/forward
start: 12
end: 17
orientation: inline

amyE 5 IS
type: ComponentDefinition
persistent identity: BBa K143001
display id: BBa_K143001
version: 1
was generated by: iGEM to SBOL conversion
description: 5??? Integration Sequence for the amyE locus of B. subtilis
created: 2008-08-26T11:00:00Z
modified: 2015-05-08T01:10:23Z
mutable provenance: The 5??? integration sequence was taken from the shuttle vector pDR111 which has been used in many studies on ''B.subtilis'', in particular in the studies of transcriptional control#1 #2 #3 <biblio> #1 pmid=14597697 #2 pmid=15937167 #3 pmid=12169614 </biblio>
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: The 5' integration sequence can be added to the front of a Biobrick construct and the 3' integration sequence specific for this locus (Part BBa_K143002) to the rear of the Biobrick construct to allow integration of the Biobrick construct into the chromosome of the gram positive bacterium B.subtilis. The AmyE locus was the first locus used for integration into ''B.subtilis'' by Shimotsu and Henner#1 and is still commonly used in vectors such as pDR111#2, pDL#3 and their derivatives. Integration at the AmyE locus removes the ability of ''B.subtilis'' to break down starch, which can be assayed with iodine as described by Cutting and Vander-horn#4. The 5' and 3' integration sequences for the AmyE locus were used to integrate the Imperial 2008 iGEM project primary construct into the ''B.sutbilis'' chromosome. <biblio> #1 pmid=3019840 #2 pmid=14597697 #3 ''Bacillus'' Genetic Stock Center [www.bgsc.org] #4 Cutting, S M.; Vander-Horn, P B. Genetic analysis. In: Harwood C R, Cutting S M. , editors. Molecular biological methods for Bacillus. Chichester, England: John Wiley & Sons, Ltd.; 1990. pp. 27???74. </biblio>
discontinued: false
dominant: false
experience: experience/Works
group u list: _199_
m user id: 0
owner id: 3475
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable notes: The AmyE integration sequence was taken from the vector after comparison by BLAST to the ''B.subtilis'' chromosome to identify the homologous sequences. The sequence present in both the host chromosome and the plasmid at the 5' end of the gene is the 5' sequence required for integration.
star: true
creator: Chris Hirst
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: 5' AmyE homologous sequence
sequence: https://synbiohub.org/public/igem/BBa_K143001_sequence/1

5' AmyE homologous sequence
type: SequenceAnnotation
persistent identity: annotation1974145
display id: annotation1974145
version: 1
top level: amyE 5 IS
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K143001/annotation1974145/range1974145/1

https://synbiohub.org/public/igem/BBa_K143001/annotation1974145/range1974145/1
type: Range
persistent identity: range1974145
display id: range1974145
version: 1
top level: amyE 5 IS
direction: direction/forward
start: 1
end: 522
orientation: inline

scar
type: ComponentDefinition
persistent identity: BBa G0006
display id: BBa_G0006
version: 1
was generated by: iGEM to SBOL conversion
description: XbaI/NheI mixed site
created: 2007-02-26T12:00:00Z
modified: 2015-08-31T04:07:27Z
mutable provenance: XbaI and NheI
owned by: james
owned by: myers
mutable description: XbaI/NheI mixed site
discontinued: false
dominant: false
experience: experience/None
group u list: _41_
m user id: 0
owner id: 126
owning group id: 70
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: None.
star: false
creator: Reshma Shetty
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_G0006_sequence/1

HpaI
type: ComponentDefinition
persistent identity: BBa M31950
display id: BBa_M31950
version: 1
was generated by: iGEM to SBOL conversion
description: HpaI restriction enzyme site
created: 2007-02-27T12:00:00Z
modified: 2015-05-08T01:14:01Z
mutable provenance: standard, restriction site
owned by: james
owned by: myers
mutable description: This is a restriction site.
discontinued: false
dominant: false
experience: experience/None
group u list: _102_
m user id: 0
owner id: 1381
owning group id: 102
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: none
star: false
creator: Rebecca Adams
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_M31950_sequence/1

BBa_K1614009
type: ComponentDefinition
persistent identity: BBa K1614009
display id: BBa_K1614009
version: 1
was generated by: iGEM to SBOL conversion
description: software generated kanamycin aptamer (candidate 1)
creator: Stefan Holderbach
created: 2015-09-15T11:00:00Z
modified: 2015-09-16T03:19:18Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2031_
m user id: 12380
owner id: 12380
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Aptamer binding kanamycin created by the MAWS Software from iGEM Team Heidelberg
mutable notes: For further details on the in silico aptamer generation see:
mutable provenance: MAWS software from iGEM Team Heidelberg (created with the pre-alpha version)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: Kanamycin aptamer
sequence: https://synbiohub.org/public/igem/BBa_K1614009_sequence/1

Kanamycin aptamer
type: SequenceAnnotation
persistent identity: annotation2458458
display id: annotation2458458
version: 1
top level: BBa_K1614009
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1614009/annotation2458458/range2458458/1

https://synbiohub.org/public/igem/BBa_K1614009/annotation2458458/range2458458/1
type: Range
persistent identity: range2458458
display id: range2458458
version: 1
direction: direction/forward
top level: BBa_K1614009
start: 1
end: 7
orientation: inline

BsrG1
type: ComponentDefinition
persistent identity: BBa M31995
display id: BBa_M31995
version: 1
was generated by: iGEM to SBOL conversion
description: BsrGI restriction enzyme site
created: 2007-02-27T12:00:00Z
modified: 2015-05-08T01:14:01Z
mutable provenance: Bacillus stearothermophilus GR75 (Z. Chen)
owned by: james
owned by: myers
mutable description: BsrGI restriction enzyme site
discontinued: false
dominant: false
experience: experience/None
group u list: _102_
m user id: 0
owner id: 1373
owning group id: 102
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: none
star: false
creator: Tiffany Guo
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_M31995_sequence/1

Scar25 ATG
type: ComponentDefinition
persistent identity: BBa K1319106
display id: BBa_K1319106
version: 1
was generated by: iGEM to SBOL conversion
description: RFC 25 Translation Start Scar Sequence
created: 2014-10-02T11:00:00Z
modified: 2015-05-08T01:09:51Z
mutable provenance: This sequence is produced by an assembly method.
owned by: james
owned by: myers
mutable description: This sequence is produced by RFC10-assembly of a RFC10-RBS with a RFC25-prefixed part.
discontinued: false
dominant: false
experience: experience/None
group u list: _1694_
m user id: 0
owner id: 19522
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: The resulting sequence is a combination of the RFC10 RBS-CDS scar (tactag) and 9 nucleotides from the RFC25 prefix (atg gcc ggc).
star: false
creator: Michael Osthege
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_K1319106_sequence/1

start
type: SequenceAnnotation
persistent identity: annotation2393317
display id: annotation2393317
version: 1
top level: Scar25 ATG
role: feature/start
role: SO:0000318
location: https://synbiohub.org/public/igem/BBa_K1319106/annotation2393317/range2393317/1

https://synbiohub.org/public/igem/BBa_K1319106/annotation2393317/range2393317/1
type: Range
persistent identity: range2393317
display id: range2393317
version: 1
top level: Scar25 ATG
direction: direction/forward
start: 7
end: 9
orientation: inline

scar
type: ComponentDefinition
persistent identity: BBa G0001
display id: BBa_G0001
version: 1
was generated by: iGEM to SBOL conversion
description: XbaI/SpeI mixed site, reverse
created: 2007-02-26T12:00:00Z
modified: 2015-08-31T04:07:27Z
mutable provenance: XbaI and SpeI
owned by: james
owned by: myers
mutable description: XbaI/SpeI mixed site, reverse
discontinued: false
dominant: false
experience: experience/None
group u list: _41_
m user id: 0
owner id: 126
owning group id: 70
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: None.
star: false
creator: Reshma Shetty
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_G0001_sequence/1

O4
type: ComponentDefinition
persistent identity: BBa K2054004
display id: BBa_K2054004
version: 1
was generated by: iGEM to SBOL conversion
description: Oligo 4 of DNA Tetrahedral Nanostructure
creator: LAI HEI WAI
created: 2016-10-13T11:00:00Z
modified: 2016-10-27T11:57:02Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2522_
m user id: 0
m user id: 30316
owner id: 30316
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This will be used to build our desired nanostructure that target micro RNA biomarkers. This plasmid encodes the second ssDNA that will make the tetrahedral nanostructure, together with oligo 1, 2, 3 and 5.
mutable notes: The assembly of the structure.
mutable provenance: Elbaz, J., Yin, P., & Voigt, C. A. (2016). Genetic encoding of DNA nanostructures and their self-assembly in living bacteria. Nature communications, 7.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_K2054004_sequence/1

BBa_K1636000
type: ComponentDefinition
persistent identity: BBa K1636000
display id: BBa_K1636000
version: 1
was generated by: iGEM to SBOL conversion
description: Lead-II-ions-specific aptamer
created: 2015-09-04T11:00:00Z
modified: 2015-09-05T06:51:22Z
mutable provenance: This sequence does not come from a genomic sequence. First reported by Li et al 2010, it is comprised by 16 nucleotides with a high percentage of G which are the ones capable of stabilising and forming the quadruplex with the lead ion.
owned by: james
owned by: myers
mutable description: This aptameric sequence is capable of recognising and binding specifically to lead II ions. This sequence, which works as a single stranded nucleic acid molecule, is capable of forming a G-quadruplex by interacting with lead ions (Pb2+) while disrupting a partially complementary duplex.
discontinued: false
dominant: false
experience: experience/None
group u list: _2053_
m user id: 25405
owner id: 25405
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: For standardisation purposes and its usage in iGEM, this sequence was cloned as a duplex on pSB1C3 thereby adding the prefix and suffix sequences to it. Given the fact that the sequence is need as a single-strand molecule, a primer was designed in order to carry out a PCR with a single primer for the production of this molecule.
star: false
creator: Roger Milton Rubio Sanchez, Ana Laura Torres
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_K1636000_sequence/1

BBa_K1680005
type: ComponentDefinition
persistent identity: BBa K1680005
display id: BBa_K1680005
version: 1
was generated by: iGEM to SBOL conversion
description: loxP Site
creator: Katharina Sporbeck
created: 2015-09-16T11:00:00Z
modified: 2015-10-28T06:30:39Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2098_
m user id: 26366
owner id: 26460
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This part contains the WT loxP site, which is recognised by Cre recombinase (Part BBa_K1680007), in forward orientation.
mutable notes: Part is shipped in RFC10.
mutable provenance: gBlock designed by Team Tuebingen
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_K1680005_sequence/1

BBa_I13457
type: ComponentDefinition
persistent identity: BBa I13457
display id: BBa_I13457
version: 1
was generated by: iGEM to SBOL conversion
description: Improved insertion site with two RNAse E cut sites
created: 2005-07-18T11:00:00Z
modified: 2015-08-31T04:07:34Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Shortened RNAse E site, insertion site, duplicate RNAse E site, and a trailing 5' hairpin (HP16 from Smolke et al., 2002).
discontinued: false
dominant: false
experience: experience/None
group u list: _11_
m user id: 0
owner id: 253
owning group id: 6
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: jkm
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: RNAse E cut site (between the bases)
sequence annotation: Hairpin
sequence annotation: RNAse E site
sequence annotation: Insertion site
sequence: https://synbiohub.org/public/igem/BBa_I13457_sequence/1

RNAse E cut site (between the bases)
type: SequenceAnnotation
persistent identity: annotation1588511
display id: annotation1588511
version: 1
top level: BBa_I13457
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I13457/annotation1588511/range1588511/1

https://synbiohub.org/public/igem/BBa_I13457/annotation1588511/range1588511/1
type: Range
persistent identity: range1588511
display id: range1588511
version: 1
top level: BBa_I13457
direction: direction/forward
start: 9
end: 10
orientation: inline

Hairpin
type: SequenceAnnotation
persistent identity: annotation1588515
display id: annotation1588515
version: 1
top level: BBa_I13457
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_I13457/annotation1588515/range1588515/1

https://synbiohub.org/public/igem/BBa_I13457/annotation1588515/range1588515/1
type: Range
persistent identity: range1588515
display id: range1588515
version: 1
top level: BBa_I13457
direction: direction/forward
start: 77
end: 111
orientation: inline

RNAse E site
type: SequenceAnnotation
persistent identity: annotation1588510
display id: annotation1588510
version: 1
top level: BBa_I13457
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I13457/annotation1588510/range1588510/1

https://synbiohub.org/public/igem/BBa_I13457/annotation1588510/range1588510/1
type: Range
persistent identity: range1588510
display id: range1588510
version: 1
top level: BBa_I13457
direction: direction/forward
start: 1
end: 21
orientation: inline

RNAse E cut site (between the bases)
type: SequenceAnnotation
persistent identity: annotation1588514
display id: annotation1588514
version: 1
top level: BBa_I13457
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I13457/annotation1588514/range1588514/1

https://synbiohub.org/public/igem/BBa_I13457/annotation1588514/range1588514/1
type: Range
persistent identity: range1588514
display id: range1588514
version: 1
top level: BBa_I13457
direction: direction/forward
start: 64
end: 65
orientation: inline

Insertion site
type: SequenceAnnotation
persistent identity: annotation1588512
display id: annotation1588512
version: 1
top level: BBa_I13457
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I13457/annotation1588512/range1588512/1

https://synbiohub.org/public/igem/BBa_I13457/annotation1588512/range1588512/1
type: Range
persistent identity: range1588512
display id: range1588512
version: 1
top level: BBa_I13457
direction: direction/forward
start: 22
end: 55
orientation: inline

RNAse E site
type: SequenceAnnotation
persistent identity: annotation1588513
display id: annotation1588513
version: 1
top level: BBa_I13457
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I13457/annotation1588513/range1588513/1

https://synbiohub.org/public/igem/BBa_I13457/annotation1588513/range1588513/1
type: Range
persistent identity: range1588513
display id: range1588513
version: 1
top level: BBa_I13457
direction: direction/forward
start: 56
end: 76
orientation: inline

Message
type: ComponentDefinition
persistent identity: BBa K361001
display id: BBa_K361001
version: 1
was generated by: iGEM to SBOL conversion
description: Message gene template
created: 2010-10-23T11:00:00Z
modified: 2015-05-08T01:12:12Z
mutable provenance: This artificial DNA sequence is converted from text with the use of ASCII system. The specific repeat sequence "repeat 1" and "repeat 2" are come from transfer region in R64 plasmid, with genomic assession no.:AB027308.1
owned by: james
owned by: myers
mutable description: This message system consists of a unique designated message sequence, being inserted with specific repeat sequence for recognition site by Rci site-specific recombinase.
discontinued: false
dominant: false
experience: experience/None
group u list: _476_
m user id: 0
owner id: 6123
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: This DNA sequence is acting as a template, to guide the make of a message inserted with specific repeat sequence for recognition by Rci recombinase in order to accomplish random rearrangement of message in encryption process.
star: false
creator: Jacky, Fong Chuen, Loo
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: repeat 1
sequence annotation: Footer
sequence annotation: repeat 2
sequence annotation: Header
sequence: https://synbiohub.org/public/igem/BBa_K361001_sequence/1

repeat 1
type: SequenceAnnotation
persistent identity: annotation2095198
display id: annotation2095198
version: 1
top level: Message
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K361001/annotation2095198/range2095198/1

https://synbiohub.org/public/igem/BBa_K361001/annotation2095198/range2095198/1
type: Range
persistent identity: range2095198
display id: range2095198
version: 1
top level: Message
direction: direction/forward
start: 21
end: 51
orientation: inline

Footer
type: SequenceAnnotation
persistent identity: annotation2095201
display id: annotation2095201
version: 1
top level: Message
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K361001/annotation2095201/range2095201/1

https://synbiohub.org/public/igem/BBa_K361001/annotation2095201/range2095201/1
type: Range
persistent identity: range2095201
display id: range2095201
version: 1
top level: Message
direction: direction/forward
start: 363
end: 426
orientation: inline

repeat 2
type: SequenceAnnotation
persistent identity: annotation2095200
display id: annotation2095200
version: 1
top level: Message
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K361001/annotation2095200/range2095200/1

https://synbiohub.org/public/igem/BBa_K361001/annotation2095200/range2095200/1
type: Range
persistent identity: range2095200
display id: range2095200
version: 1
top level: Message
direction: direction/forward
start: 332
end: 362
orientation: inline

Header
type: SequenceAnnotation
persistent identity: annotation2095196
display id: annotation2095196
version: 1
top level: Message
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K361001/annotation2095196/range2095196/1

https://synbiohub.org/public/igem/BBa_K361001/annotation2095196/range2095196/1
type: Range
persistent identity: range2095196
display id: range2095196
version: 1
top level: Message
direction: direction/forward
start: 13
end: 20
orientation: inline

BBa_J70050
type: ComponentDefinition
persistent identity: BBa J70050
display id: BBa_J70050
version: 1
was generated by: iGEM to SBOL conversion
description: Left Library Insert Preparation Part (To use with J70052, and J70040)
created: 2009-01-22T12:00:00Z
modified: 2015-05-08T01:08:20Z
mutable provenance: Designed
owned by: james
owned by: myers
mutable description: Still in Testing Surround a part with J70050 and J70052, digest with AarI Insert into a vector that contained J70040, and had J70040 removed by AarI and MabI
discontinued: false
dominant: false
experience: experience/None
group u list: _41_
m user id: 0
owner id: 1201
owning group id: 41
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Not sure if single AarI site will be good enough
star: false
creator: Julie Norville
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: Spacer
sequence annotation: AarI
sequence: https://synbiohub.org/public/igem/BBa_J70050_sequence/1

Spacer
type: SequenceAnnotation
persistent identity: annotation2000145
display id: annotation2000145
version: 1
top level: BBa_J70050
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_J70050/annotation2000145/range2000145/1

https://synbiohub.org/public/igem/BBa_J70050/annotation2000145/range2000145/1
type: Range
persistent identity: range2000145
display id: range2000145
version: 1
top level: BBa_J70050
direction: direction/forward
start: 8
end: 11
orientation: inline

AarI
type: SequenceAnnotation
persistent identity: annotation2000144
display id: annotation2000144
version: 1
top level: BBa_J70050
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J70050/annotation2000144/range2000144/1

https://synbiohub.org/public/igem/BBa_J70050/annotation2000144/range2000144/1
type: Range
persistent identity: range2000144
display id: range2000144
version: 1
top level: BBa_J70050
direction: direction/forward
start: 1
end: 7
orientation: inline

BBa_K106017
type: ComponentDefinition
persistent identity: BBa K106017
display id: BBa_K106017
version: 1
was generated by: iGEM to SBOL conversion
description: AarI "D" site scar sequence
created: 2008-10-23T11:00:00Z
modified: 2015-05-08T01:08:58Z
mutable provenance: For registry assembly only.
owned by: james
owned by: myers
mutable description: For registry assembly only.
discontinued: false
dominant: false
experience: experience/None
group u list: _226_
m user id: 0
owner id: 2659
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: For registry assembly only.
star: false
creator: Andrew Horwitz
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_K106017_sequence/1

OriTF
type: ComponentDefinition
persistent identity: BBa J01002
display id: BBa_J01002
version: 1
was generated by: iGEM to SBOL conversion
description: OriT-F (Origin of Transfer for the F-plasmid nic region)
created: 2005-10-17T11:00:00Z
modified: 2015-08-31T04:08:11Z
owned by: james
owned by: myers
mutable description: OriTf (Origin of Transfer for the F-type conjugative plasmid) is the nic region, is where the relaxosome nicks the plasmid and conjugative transfer by F plasmid machinery begins via rolling circle replication. This particular plasmid has been derived from a F-type plasmid donated by Professor Laura Frost University of Alberta. NOTE: OriTf is NOT the same as the host cell's native Origin of Transfer (this is a separate site within the bacterial host's main genome).
discontinued: false
dominant: false
experience: experience/Works
group u list: _13_
m user id: 0
owner id: 395
owning group id: 13
sample status: It's complicated
status: status/Planning
bookmark: false
star: true
creator: Golden Bear
type: DnaRegion
role: partType/Conjugation
role: SO:0000724
sequence: https://synbiohub.org/public/igem/BBa_J01002_sequence/1

7-fingers
type: ComponentDefinition
persistent identity: BBa J35007
display id: BBa_J35007
version: 1
was generated by: iGEM to SBOL conversion
description: 7-fingers arm
created: 2006-10-22T11:00:00Z
modified: 2015-08-31T04:08:47Z
mutable provenance: artificial sequence
owned by: james
owned by: myers
mutable description: DNA ORIGAMI BioBrick
discontinued: false
dominant: false
experience: experience/None
group u list: _64_
m user id: 0
owner id: 807
owning group id: 64
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: OligoAnalyzer 3.0 http://www.idtdna.com/analyzer/Applications/OligoAnalyzer/
star: false
creator: Andrey Kuznetsov
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_J35007_sequence/1

https://synbiohub.org/public/igem/BBa_I13450_sequence/1
type: Sequence
persistent identity: BBa I13450 sequence
display id: BBa_I13450_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: myers
owned by: james
elements: tcaattgaggtagaggtacacacgcgaactccgatagccaattcagagtaataaactgtgataatcaatgcatg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M31950_sequence/1
type: Sequence
persistent identity: BBa M31950 sequence
display id: BBa_M31950_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gtt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J35005_sequence/1
type: Sequence
persistent identity: BBa J35005 sequence
display id: BBa_J35005_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cgccgtgcagttttctgctctcgttttcgagtcgcgttttcgcgtggagttttctcctctggttttccagtcggcg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K592202_sequence/1
type: Sequence
persistent identity: BBa K592202 sequence
display id: BBa_K592202_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tactagtagcggccgctgcaggagtcactaagggttagttagttagattagcagaaagtcaaaagcctccgaccggaggcttttgactaaaacttcccttggggttatcattggggctcactcaaaggcggtaatcagataaaaaaaatccttagctttcgctaaggatgatttctgctagagatggaatagactggatggaggcggataaagttgcaggaccacttctgcgctcggcccttccggctggctggtttattgctgataaatctggagccggtgagcgtgggtctcgcggtatcattgcagcactggggccagatggtaagccctcccgtatcgtagttatctacacgacggggagtcaggcaactatggatgaacgaaatagacagatcgctgagataggtgcctcactgattaagcattggtaactgtcagaccaagtttactcatatatactttagattgatttaaaacttcatttttaatttaaaaggatctaggtgaagatcctttttgataatctcatgaccaaaatcccttaacgtgagttttcgttccactgagcgtcagaccccttaataagatgatcttcttgagatcgttttggtctgcgcgtaatctcttgctctgaaaacgaaaaaaccgccttgcagggcggtttttcgaaggttctctgagctaccaactctttgaaccgaggtaactggcttggaggagcgcagtcaccaaaacttgtcctttcagtttagccttaaccggcgcatgacttcaagactaactcctctaaatcaattaccagtggctgctgccagtggtgcttttgcatgtctttccgggttggactcaagacgatagttaccggataaggcgcagcggtcggactgaacggggggttcgtgcatacagtccagcttggagcgaactgcctacccggaactgagtgtcaggcgtggaatgagacaaacgcggccataacagcggaatgacaccggtaaaccgaaaggcaggaacaggagagcgcacgagggagccgccaggggaaacgcctggtatctttatagtcctgtcgggtttcgccaccactgatttgagcgtcagatttcgtgatgcttgtcaggggggcggagcctatggaaaaacggctttgccgcggccctctcacttccctgttaagtatcttcctggcatcttccaggaaatctccgccccgttcgtaagccatttccgctcgccgcagtcgaacgaccgagcgtagcgagtcagtgagcgaggaagcggaatatatcctgtatcacatattctgctgacgcaccggtgcagccttttttctcctgccacatgaagcacttcactgacaccctcatcagtgccaacatagtaagccagtatacactccgctagcgctgaggtctgcctcgtgaagaaggtgttgctgactcataccaggcctgaatcgccccatcatccagccagaaagtgagggagccacggttgatgagagctttgttgtaggtggaccagttggtgattttgaacttttgctttgccacggaacggtctgcgttgtcgggaagatgcgtgatctgatccttcaactcagcaaaagttcgatttattcaacaaagccacgttgtgtctcaaaatctctgatgttacattgcacaagataaaaatatatcatcatgaacaataaaactgtctgcttacataaacagtaatacaaggggtgtttactagaggcgtcgacgccatggtccatatgaatatcctccttagttcctattccgaagttcctattctctagaaagtataggaacttcagagcgcttttgaagctggggtgggcgaagaactccagcatgagatccccgcgctggaggatcatccagccggcgtcccggaaaacgattccgaagcccaacctttcatagaaggcggcggtggaatcgaaatctcgtgatggcaggttgggcgtcgcttggtcggtcatttcg…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M31962_sequence/1
type: Sequence
persistent identity: BBa M31962 sequence
display id: BBa_M31962_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccatc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M31988_sequence/1
type: Sequence
persistent identity: BBa M31988 sequence
display id: BBa_M31988_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: myers
owned by: james
elements: acgcgt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I714032_sequence/1
type: Sequence
persistent identity: BBa I714032 sequence
display id: BBa_I714032_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tttacgctgagatgataggatgccatcgtgttttatcccgctgaagggcgcacgtttctgaacgaagtgaagaaagtctaagtgcgccctgataaataaaagagttatcagggattgtagtgggatttgac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_G0006_sequence/1
type: Sequence
persistent identity: BBa G0006 sequence
display id: BBa_G0006_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ctctagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M31955_sequence/1
type: Sequence
persistent identity: BBa M31955 sequence
display id: BBa_M31955_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gggccc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I13455_sequence/1
type: Sequence
persistent identity: BBa I13455 sequence
display id: BBa_I13455_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccgcggtgaaaatacgagaatattatttgtattgatctcctagggaattaggtcttcgaatccgatccattgcagtgg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M31967_sequence/1
type: Sequence
persistent identity: BBa M31967 sequence
display id: BBa_M31967_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ctag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K245009_sequence/1
type: Sequence
persistent identity: BBa K245009 sequence
display id: BBa_K245009_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccggc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M31964_sequence/1
type: Sequence
persistent identity: BBa M31964 sequence
display id: BBa_M31964_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gtatcc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M31956_sequence/1
type: Sequence
persistent identity: BBa M31956 sequence
display id: BBa_M31956_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: agcgct
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J01003_sequence/1
type: Sequence
persistent identity: BBa J01003 sequence
display id: BBa_J01003_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gccgccttttcctcaatcgctcttcgttcgtctggaaggcagtacaccttgataggtgggctgcccttcctggttggcttggtttcatcagccatccgcttgccctcatctgttacgccggcggtagccggccagcctcgcagagcaggattcccgttgagcaccgccaggtgcgaataagggacagtgaagaaggaacacccgctcgcgggtgggcctacttcacctatcctgcccggctgacgccgttggatacaccaaggaaagtctacacgaaccctttggcaaaatcctgtatatcgtgcgaaaaaggatggatataccgaaaaaatcgctataatgaccccgaagcagggttatgcagcggaaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I11080_sequence/1
type: Sequence
persistent identity: BBa I11080 sequence
display id: BBa_I11080_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cgtgggccctca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M31984_sequence/1
type: Sequence
persistent identity: BBa M31984 sequence
display id: BBa_M31984_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gggccc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I11079_sequence/1
type: Sequence
persistent identity: BBa I11079 sequence
display id: BBa_I11079_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: acaacgcgtgta
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I13457_sequence/1
type: Sequence
persistent identity: BBa I13457 sequence
display id: BBa_I13457_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tattatttgtattgatctggtgaattaggtcttcgaatccgatccattgcagtggtattatttgtattgatctccttactatctctcgagagattagtacctttggagatc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K150008_sequence/1
type: Sequence
persistent identity: BBa K150008 sequence
display id: BBa_K150008_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ggatcc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J70036_sequence/1
type: Sequence
persistent identity: BBa J70036 sequence
display id: BBa_J70036_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atagcaggtggacaagaggagtcccgggagctggaactcccacctgcatat
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I13452_sequence/1
type: Sequence
persistent identity: BBa I13452 sequence
display id: BBa_I13452_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gaattaggtcttcgaatcacaaagattagtcacacgcgatccattgcagtgg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I732007_sequence/1
type: Sequence
persistent identity: BBa I732007 sequence
display id: BBa_I732007_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gaagatcttccggatccgg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0103_sequence/1
type: Sequence
persistent identity: BBa B0103 sequence
display id: BBa_B0103_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgtgaagagctg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0105_sequence/1
type: Sequence
persistent identity: BBa B0105 sequence
display id: BBa_B0105_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: accggc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1319107_sequence/1
type: Sequence
persistent identity: BBa K1319107 sequence
display id: BBa_K1319107_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: accggttaatactagag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I11081_sequence/1
type: Sequence
persistent identity: BBa I11081 sequence
display id: BBa_I11081_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: caaggatcc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0045_sequence/1
type: Sequence
persistent identity: BBa B0045 sequence
display id: BBa_B0045_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gctagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K259002_sequence/1
type: Sequence
persistent identity: BBa K259002 sequence
display id: BBa_K259002_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atctcaagagtggcagcggtcttgagtggcagcggcggtatacggcagcggtatgtaactagctcctcagtggcagcggtgaggaggc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J70040_sequence/1
type: Sequence
persistent identity: BBa J70040 sequence
display id: BBa_J70040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gcaggtggacacctagggtcccgggagcaccaggtcccacctgca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K259004_sequence/1
type: Sequence
persistent identity: BBa K259004 sequence
display id: BBa_K259004_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atctcaagagtggcagcggtcttgagtggcagcggcggtatacggcagcggtatgtaagtagtaacaagtagcgtggggca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J35120_sequence/1
type: Sequence
persistent identity: BBa J35120 sequence
display id: BBa_J35120_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tggacactaagctattcatc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M31995_sequence/1
type: Sequence
persistent identity: BBa M31995 sequence
display id: BBa_M31995_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: myers
owned by: james
elements: tgtaca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1973000_sequence/1
type: Sequence
persistent identity: BBa K1973000 sequence
display id: BBa_K1973000_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatgctgctgggcagtcttctgctgggcggcttgcacgcggattgggatttctcccagatcagccgcaagtcgcaggctctgtacggcccactaggtgccgggcagggtcgcatcgatgcctggcagagcctgatggccacgcagaagcagggtaccgagcttgaaaggttgcaagtggtcaaccgcttcttcaaccagcaattgcggtatgtcgaggatatcgatctgtggcatgaggtcgactactgggccacacctgtgcaggcactgatcaaaggtgcaggggactgcgaggattacgccatcgccaaatatttcagtctacggcgcatgggaatccccagcgaaaaactgcgcatcacctacgtcaaggcgctgcggcagaaccgggcacacatggtcctgacctattattcaagcccacaggcccagcctttggtgctggacagtctgatggatgccataaagccggcaagccagcgtaccgacctgctgccggtgtacgccttcaatggcgaggggttgtggctgacgggcgcggcagggaacaagaaggttggtgataccaagcggctgtcacgctggcaggatttgttgaagaaaatgcaggccgaagggttcccggccgagccggtttactaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0040_sequence/1
type: Sequence
persistent identity: BBa B0040 sequence
display id: BBa_B0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aggttctgttaagtaactgaacccaatgtcgttagtgacgcttacctcttaagaggtcactgacctaaca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I11071_sequence/1
type: Sequence
persistent identity: BBa I11071 sequence
display id: BBa_I11071_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atcggtaccgca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J70052_sequence/1
type: Sequence
persistent identity: BBa J70052 sequence
display id: BBa_J70052_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atagcaggtg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_G00000_sequence/1
type: Sequence
persistent identity: BBa G00000 sequence
display id: BBa_G00000_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gaattcgcggccgcttctagag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K809002_sequence/1
type: Sequence
persistent identity: BBa K809002 sequence
display id: BBa_K809002_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gtaatagggggagggggtgggtgataataaccagaatattcaataaatacagagcacacattacataaattttataatataaccaatataaaataaaattaaaataattaatatatatatataaatataataaattattatatataaatatatataatttttataataaatattataatattatataaataaataattataatatataataaatatataataataataaaaatattaacaatataataaaaatttataatataaatataaattataaataagttaaattaataaaataataaatgttaacaagaagatatctggggtcccattaataattattattttcaataataattgggaccccccaccattata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J70031_sequence/1
type: Sequence
persistent identity: BBa J70031 sequence
display id: BBa_J70031_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: accaggttactagagacctggt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I714030_sequence/1
type: Sequence
persistent identity: BBa I714030 sequence
display id: BBa_I714030_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cctctggtgactttatccgtaaataatttaacccactccacaaaaaggctcaacaggttggtggttctcaccaccaaaagcaccacaccccacgcaaaaacaagtttttgctgatttttctttataaatagagtgttatgaaaaattagtttctcttactctctttatgatatttaaaaaagcggtgtcggcgcggctacaacaacgcgccgacaccgttttgtaggggtggtactgactatttttataaaaaacattattttatattaggggtgctgctagcggcgcggtgtgtttttttataggataccgctaggggcgctgctagcggtgcgtccctgtttgcattatgaat
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1930003_sequence/1
type: Sequence
persistent identity: BBa K1930003 sequence
display id: BBa_K1930003_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gaccaagcctgcaaaaacaaagtgcaaagatatcagtaaggtcttaaaggccgaagcggtggcctaagataaaactgggcgccctggcgttagttccgcagaagataaaactgggctaaggcatcaaagaaggtatctaaatccgaagtagttagctaagtgattggcgggcgaaggaatttgctaagctatgaaggattggactaagccattcccgaagctatcacataagcgatgacatgcaccgcagcagacaggtacaaataagaaaactttgaagtctttaagaattagtattaagaagtgatgaagggctatgccaccctcctagagacagctataagctcttaaggagtgaagctgttgaataaggcgtagtagaagcaattaagtcctgaagttctgaagctgttgacgaagataaaactctgaataataagacctactataagtgctgaagcgttgaagagataagacagagaagtcttgaagtgcttaaagaagccttcaactaagtgattacactgctaagagatgaagcattgcacccaccgactccggcaaaaaagggcaaggtgtcaattctcatgtttgacagcttatcatcggcaatagttacccttattatcaagataagaaagaaaaggatttttcgctacgctcaaatcctttaaaaaaacacaaaagaccacattttttaatgtggtctttattcttcaactaaagcacccattagttcaacaaacgaaaattggataaagtgggatatttttaaaatatatatttatgttacagtaatattgacttttaaaaaaggattgattctaatgaagaaagcagacaagtaagcctcctaaattcactttagataaaaatttaggaggcatatcaaatgaactttaataaaattgatttagacaattggaagagaaaagagatatttaatcattatttgaaccaacaaacgacttttagtataaccacagaaattgatattagtgttttataccgaaacataaaacaagaaggatataaattttaccctgcatttattttcttagtgacaagggtgataaactcaaatacagcttttagaactggttacaatagcgacggagagttaggttattgggataagttagagccactttatacaatttttgatggtgtatctaaaacattctctggtatttggactcctgtaaagaatgacttcaaagagttttatgatttatacctttctgatgtagagaaatataatggttcggggaaattgtttcccaaaacacctatacctgaaaatgctttttctctttctattattccatggacttcatttactgggtttaacttaaatatcaataataatagtaattaccttctacccattattacagcaggaaaattcattaataaaggtaattcaatatatttaccgctatctttacaggtacatcattctgtttgtgatggttatcatgcaggattgtttatgaactctattcaggaattgtcagataggcctaatgactggcttttataatatgagataatgccgactgtactttttacagtcggttttctaatgtcactaacctgccccgttagttgaagaaggtttttatattacagctccagatcctctacgccggacgcatcgtggccggcatcaccggcgccacaggtgcggttgctggcgcctatatcgccgacatcaccgatggggaagatcgggctcgccacttcgggctcatgagcgcttgtttcggcgtgggtatggtggcaggccccgtggccgggggactgttgggcgccatctccttgcatgcaccattccttgcggcggcggtgctcaacggcctcaacctactactgggctgcttcctaatgcaggagtcgcataagggagagcgtcgacatggatgagcgatgatgatatccgtttaggctgggcggtgatagcttctcgttcaggcagtacgcctcttttcttttccaga…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_G00102_sequence/1
type: Sequence
persistent identity: BBa G00102 sequence
display id: BBa_G00102_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gctcactcaaaggcggtaat
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I11082_sequence/1
type: Sequence
persistent identity: BBa I11082 sequence
display id: BBa_I11082_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ggatctagacc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K106016_sequence/1
type: Sequence
persistent identity: BBa K106016 sequence
display id: BBa_K106016_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ggtagttcccta
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I11075_sequence/1
type: Sequence
persistent identity: BBa I11075 sequence
display id: BBa_I11075_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cttgctagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I11078_sequence/1
type: Sequence
persistent identity: BBa I11078 sequence
display id: BBa_I11078_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gcaccgcggcga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J61021_sequence/1
type: Sequence
persistent identity: BBa J61021 sequence
display id: BBa_J61021_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ctgtctcttatacacatct
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K361001_sequence/1
type: Sequence
persistent identity: BBa K361001 sequence
display id: BBa_K361001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gggcccagatctagaaagatggcaatactttcgtgccaatccggtacgtggtttgtcttacaatcgttgtttgagtgtaacaatcgatctttcattgactcgcacaatgtatcggacaatcgatccttgtctcttacaatgtatcggtctgtctttgtatccatctttgacacaatcattgagacaatcactgactcggtgtatccatctttgactgagacaatcggtgacacaatgaatctttgactccttgagtccaacaatgtatcggtctgtctttgtatccatctttgacacaatcattgagacaatgtatcggtcggtcgatgagctgctggcctacgtgccaatccggtacgtggagtgagaataagagagagagagaaagacagcaagtgagtaagctagcaagatagactaatagtcggatccctcgag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M31982_sequence/1
type: Sequence
persistent identity: BBa M31982 sequence
display id: BBa_M31982_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: myers
owned by: james
elements: gcggccgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0104_sequence/1
type: Sequence
persistent identity: BBa B0104 sequence
display id: BBa_B0104_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cctaga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K510000_sequence/1
type: Sequence
persistent identity: BBa K510000 sequence
display id: BBa_K510000_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tactagtagcggccgctgcagtccggcaaaaaaacgggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaagattacttcgcgttatgcaggcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaataggccaaccagataagtgaaatctagttccaaactattttgtcatttttaattttcgtattagcttacgacgctacacccagttcccatctattttgtcactcttccctaaataatccttaaaaactccatttccacccctcccagttcccaactattttgtccgcccacaggccgcctaggccgtagcttggcactggccgtcgttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcgccttgcagcacatccccctttcgccagctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacagttgcgcagcctgaatggcgaatggcgcctgatgcggtattttctccttacgcatctgtgcggtatttcacaccgcatatggtgcactctcagtacaatctgctctgatgccgcatagttaagccagccccgacacccgccaacacccgctgacgcgccctgacgggcttgtctgctcccggcatccgcttacagacaagctgtgaccgtctccgggagctgcatgtgtcagaggttttcaccgtcatcaccgaaacgcgcgagacgaaagggcctcgtgatacgcctatttttataggttaatgtcatgataataatggtttcttagacgtcaggtggcacttttcggggaaatgtgcgcggaacccctatttgtttatttttctaaatacattcaaatatgtatccgctcatgagacaataaccctgataaatgcttcaataatattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattcccttttttgcggcattttgccttcctgtttttgctcacccagaaacgctggtgaaagtaaaagatgctgaagatcagttgggtgcacgagtgggttacatcgaactggatctcaacagcggtaagatccttgagagttttcgccccgaagaacgttttccaatgatgagcacttttaaagttctgctatgtggcgcggtattatcccgtattgacgccgggcaagagcaactcggtcgccgcatacactattctcagaatgacttggttgagtactcaccagtcacagaaaagcatcttacggatggcatgacagtaagagaattatgcagtgctgccataaccatgagtgataacactgcggccaacttacttctgacaacgatcggaggaccgaaggagctaaccgcttttttgcacaacatgggggatcatgtaactcgccttgatcgttgggaaccggagctgaatgaagccataccaaacgacgagcgtgacaccacgatgcctgtagcaatggcaacaacgttgcgcaaactattaactggcgaactacttactctagcttcccggcaacaattaatagactggatggaggcggataaagttgcaggaccacttctgcgctcggcccttccggctggctggtttattgctgataaatctggagccggtgagcgtgggtctcgcggtatcattgcagcactggggccagatggtaagccctcccgtatcgtagttatctacacgacggggagtcaggcaactatggatgaacgaaatagacagatcgctgagataggtgcctcactgattaagcattggtaactgtcagaccaagtttactcatatatactttagattgatttaaaacttcatttttaatttaaaaggatctaggtgaagatcctttttgataatctcatgaccaaaatcccttaacgtgagttttcgttccactgagcgtcagaccccgtagaaaagatcaaaggatcttcttga…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K175027_sequence/1
type: Sequence
persistent identity: BBa K175027 sequence
display id: BBa_K175027_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: agttacgctagggataacagggtaatatag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1319106_sequence/1
type: Sequence
persistent identity: BBa K1319106 sequence
display id: BBa_K1319106_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tactagatggccggc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J36856_sequence/1
type: Sequence
persistent identity: BBa J36856 sequence
display id: BBa_J36856_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tctgtgagacgacgcaccggtcgcaggttttgtctcacagttctttccgagccgtataaatatgcctatcattgctaatcctttccatac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M31990_sequence/1
type: Sequence
persistent identity: BBa M31990 sequence
display id: BBa_M31990_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ggcgcgcc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M31968_sequence/1
type: Sequence
persistent identity: BBa M31968 sequence
display id: BBa_M31968_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: acctgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0046_sequence/1
type: Sequence
persistent identity: BBa B0046 sequence
display id: BBa_B0046_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgcat
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K259009_sequence/1
type: Sequence
persistent identity: BBa K259009 sequence
display id: BBa_K259009_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atctcaagagtggcagcggtcttgagtggcagcggcggtatacggcagcggtatgtaagtagtaacaagtagcgtggggcag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J35002_sequence/1
type: Sequence
persistent identity: BBa J35002 sequence
display id: BBa_J35002_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gcctcttttgaggcgcaagttttcttgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J36857_sequence/1
type: Sequence
persistent identity: BBa J36857 sequence
display id: BBa_J36857_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tctgtgagacgacgcaccggtcgcaggttttgtctcacagttaaatatgcctatcattgctaatcctttccatac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1973033_sequence/1
type: Sequence
persistent identity: BBa K1973033 sequence
display id: BBa_K1973033_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcaatccgtaaacaggtcaaacatcagttgccgcaaccaaatattggctaggtccttgtggtacttcgcatgccagaacatgttgatggctatttcaggcaagacgactgggtgcggcaaggcgcttaggccgaagggctccacgcagcagtcggctaaacgtatcggcacagtggcgagcagatcggtgcgctggaggatgtggccaacggcggcgaagtgcggcacttccagacggatgtcgcgccggatgccgacccgtgtcatgtacgtgtccacctcgccgtggccggtgccagcggcgatgacacgcacgtggccgtaggaacagaagcgctccagagtcaggggttcgcgggtgactggatggtccttgcgacataggcacacgtagtgattctggagcagccggcgctgaaagaagccagtttgcagattgggaagcaggcccacggccaagtccacggttccgttctgcaaggcctgcatcaggctcatcgaactgtcgcgcaccgtactgatcacgcaattgggggcctggtgagccagcacatccatcagccgcggcatgaagtagatctcgccaatgtcggtcatggccagggtgaaggtacgctcgctggtcagcggatcgaagctttcatggtgctgtagggcgttgcgcagtgcgtgcatggccgaagtgacgggctcggccagatgcgcggcatagggtgtgggttccattccctgatgtgtgcgcacgaagagtgggtcctgtagcgaggtgcgcaggcgtttcagcgcattgctcacggcaggctgggtcaggcccaggttctccgcagtgatagagacgcgtctgtcgaccagcaactggttgaacaccaccagcaggtttaaatccaggtcacgcagttccatggggcctcgcttgggttattgctggtgcccggccgggcgcaatattcatgttgatgatttattatatatcgagtggtgtatttatcaatattgtttgctccgttatcgttattaacaagtcatcaataaagccatc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M31958_sequence/1
type: Sequence
persistent identity: BBa M31958 sequence
display id: BBa_M31958_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: attaat
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I11076_sequence/1
type: Sequence
persistent identity: BBa I11076 sequence
display id: BBa_I11076_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tacaagcttct
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K649204_sequence/1
type: Sequence
persistent identity: BBa K649204 sequence
display id: BBa_K649204_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ataacttcgtataggatactttatacgaagttat
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_G0001_sequence/1
type: Sequence
persistent identity: BBa G0001 sequence
display id: BBa_G0001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ctctagta
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J61022_sequence/1
type: Sequence
persistent identity: BBa J61022 sequence
display id: BBa_J61022_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: agatgtgtataagagacag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K106017_sequence/1
type: Sequence
persistent identity: BBa K106017 sequence
display id: BBa_K106017_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tgcgggatcctgagtaaataagcggccgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J70032_sequence/1
type: Sequence
persistent identity: BBa J70032 sequence
display id: BBa_J70032_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ctggttcacctggttaattaattaattaaaccaggtgaacgtggtctctactagagaccaggttactagagacctggttactagagaacacctggtacagcaggtggcgcgccggcgcgccagctggtgaacaccagg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1636000_sequence/1
type: Sequence
persistent identity: BBa K1636000 sequence
display id: BBa_K1636000_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gggtgggtgggtgggt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I11072_sequence/1
type: Sequence
persistent identity: BBa I11072 sequence
display id: BBa_I11072_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gcaaacgttcga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_G0004_sequence/1
type: Sequence
persistent identity: BBa G0004 sequence
display id: BBa_G0004_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tactagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J35004_sequence/1
type: Sequence
persistent identity: BBa J35004 sequence
display id: BBa_J35004_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cgccgtgcagttttctgctctcgttttcgagtggagttttctcctctggttttccagtcggcg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J70030_sequence/1
type: Sequence
persistent identity: BBa J70030 sequence
display id: BBa_J70030_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ctggttcacctggttaattaattaattaaaccaggtgaacgtggtctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0048_sequence/1
type: Sequence
persistent identity: BBa B0048 sequence
display id: BBa_B0048_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cctagg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M31952_sequence/1
type: Sequence
persistent identity: BBa M31952 sequence
display id: BBa_M31952_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gacgtc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K2027050_sequence/1
type: Sequence
persistent identity: BBa K2027050 sequence
display id: BBa_K2027050_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gaactagatcgcagccccacagtcgagaagcagtaagacgacatgggaacaaacatggggccaagagatctagggcacgctgtgtggatcgaggtaatcc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J70034_sequence/1
type: Sequence
persistent identity: BBa J70034 sequence
display id: BBa_J70034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gcaggtggacaagaggagtcccgggagctggaactcccacctgca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M31965_sequence/1
type: Sequence
persistent identity: BBa M31965 sequence
display id: BBa_M31965_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tgatca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J36855_sequence/1
type: Sequence
persistent identity: BBa J36855 sequence
display id: BBa_J36855_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ggttggtgtggttggaagtagtatggaaaggatta
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M31987_sequence/1
type: Sequence
persistent identity: BBa M31987 sequence
display id: BBa_M31987_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tgatca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I714031_sequence/1
type: Sequence
persistent identity: BBa I714031 sequence
display id: BBa_I714031_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gatctcgtctttctcttcggggaacaccggcacccgcaggtgctgccgccgccggcggccgtgttccttttcgtcgttctccatgcctcgcctcgtctctcatgccggcggtagccggctgcctcgcagagcaggatgacccgttgagcgcccccggcgcgaataagggacagtgaagatagataaccggctcgccggttagctaacttcacacatcctgcccgccttacggcgttaataacaccaaggaaagtctacaccagccattacgatttatc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_G00001_sequence/1
type: Sequence
persistent identity: BBa G00001 sequence
display id: BBa_G00001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tactagtagcggccgctgcag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M31989_sequence/1
type: Sequence
persistent identity: BBa M31989 sequence
display id: BBa_M31989_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gcgcgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J33207_sequence/1
type: Sequence
persistent identity: BBa J33207 sequence
display id: BBa_J33207_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtgaaattgtgagcggataacaatttcacacaggaaacagctatgaccatgattacggattcactggccgtcgttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcgccttgcagcacatccccctttcgccagctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacagttgcgcagcctgaatggcgaatggcgctttgcctggtttccggcaccagaagcggtgccggaaagctggctggagtga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K112999_sequence/1
type: Sequence
persistent identity: BBa K112999 sequence
display id: BBa_K112999_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ggatct
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1973006_sequence/1
type: Sequence
persistent identity: BBa K1973006 sequence
display id: BBa_K1973006_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tactagtagcggccgctgcagtccggcaaaaaaacgggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaagattacttcgcgttatgcaggcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaataggccaaccagataagtgaaatctagttccaaactattttgtcatttttaattttcgtattagcttacgacgctacacccagttcccatctattttgtcactcttccctaaataatccttaaaaactccatttccacccctcccagttcccaactattttgtccgcccacaggccgcctaggccgtagcttggcactggccgtcgttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcgccttgcagcacatccccctttcgccagctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacagttgcgcagcctgaatggcgaatggcgcctgatgcggtattttctccttacgcatctgtgcggtatttcacaccgcatatggtgcactctcagtacaatctgctctgatgccgcatagttaagccagccccgacacccgccaacacccgctgacgcgccctgacgggcttgtctgctcccggcatccgcttacagacaagctgtgaccgtctccgggagctgcatgtgtcagaggttttcaccgtcatcaccgaaacgcgcgagacgaaagggcctcgtgatacgcctatttttataggttaatgtcatgataataatggtttcttagacgtcaggtggcacttttcggggaaatgtgcgcggaacccctatttgtttatttttctaaatacattcaaatatgtatccgctcatgagacaataaccctgataaatgcttcaataatattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattcccttttttgcggcattttgccttcctgtttttgctcacccagaaacgctggtgaaagtaaaagatgctgaagatcagttgggtgcacgagtgggttacatcgaactggatctcaacagcggtaagatccttgagagttttcgccccgaagaacgttttccaatgatgagcacttttaaagttctgctatgtggcgcggtattatcccgtattgacgccgggcaagagcaactcggtcgccgcatacactattctcagaatgacttggttgagtactcaccagtcacagaaaagcatcttacggatggcatgacagtaagagaattatgcagtgctgccataaccatgagtgataacactgcggccaacttacttctgacaacgatcggaggaccgaaggagctaaccgcttttttgcacaacatgggggatcatgtaactcgccttgatcgttgggaaccggagctgaatgaagccataccaaacgacgagcgtgacaccacgatgcctgtagcaatggcaacaacgttgcgcaaactattaactggcgaactacttactctagcttcccggcaacaattaatagactggatggaggcggataaagttgcaggaccacttctgcgctcggcccttccggctggctggtttattgctgataaatctggagccggtgagcgtgggtctcgcggtatcattgcagcactggggccagatggtaagccctcccgtatcgtagttatctacacgacggggagtcaggcaactatggatgaacgaaatagacagatcgctgagataggtgcctcactgattaagcattggtaactgtcagaccaagtttactcatatatactttagattgatttaaaacttcatttttaatttaaaaggatctaggtgaagatcctttttgataatctcatgaccaaaatcccttaacgtgagttttcgttccactgagcgtcagaccccgtagaaaagatcaaaggatcttcttga…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J35007_sequence/1
type: Sequence
persistent identity: BBa J35007 sequence
display id: BBa_J35007_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cgccgtgcagttttctgctctcgttttcgagtcgcatttttgcgtcgccttttggcgtcgctttttagcgtggagttttctcctctggttttccagtcggcg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J36858_sequence/1
type: Sequence
persistent identity: BBa J36858 sequence
display id: BBa_J36858_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tctgtgagacgacgcaccggtcgcaggttttgtctcacagtttgctaatcctttccatac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J35003_sequence/1
type: Sequence
persistent identity: BBa J35003 sequence
display id: BBa_J35003_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gcccgcagttttctgcctcgttttcgagggagttttctccgggc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K598001_sequence/1
type: Sequence
persistent identity: BBa K598001 sequence
display id: BBa_K598001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccatgtatgttgatacttaatttaaagattaaacaaaagatgataccagccgaaaggcccttggcagctctcgaggaggtactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K175000_sequence/1
type: Sequence
persistent identity: BBa K175000 sequence
display id: BBa_K175000_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgcaggccactttcccggcgttccgcctgaaccgaaatgcactattttacatgggtctattcgctctgctggccttttttctgctggcacctcaacacgcgtttgcatcggaaggtacaggaggaagcttaccgtacgagtcgtggctgaccaacctccgcaactctgtcaccggaccagttgcgtttgcgctaagcatcatagggattgtagtggccggcggcattctaatttttgggggagagctgaatggttttcttcgcacgcttattttcatcgttcttgttatggggctcctggtcggggcgcagaacatgatgagcactttttttggccggggggcggagatcgccgcgctcacagacggtgccttacatcaggtcaaagtagcagctttggacgtagccggttttcctggcgaagctgtgatgcgctgggcagaacgcgggcatattgcaggctaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_G0005_sequence/1
type: Sequence
persistent identity: BBa G0005 sequence
display id: BBa_G0005_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gctagta
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J36859_sequence/1
type: Sequence
persistent identity: BBa J36859 sequence
display id: BBa_J36859_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tctgtgagacgacgcaccggtcgcaggttttgtctcacagttgctttccattctacatatgaaggttggtgtggttggaagtaatgtagaatggaaag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0102_sequence/1
type: Sequence
persistent identity: BBa B0102 sequence
display id: BBa_B0102_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gctcttct
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M31986_sequence/1
type: Sequence
persistent identity: BBa M31986 sequence
display id: BBa_M31986_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cggccg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M31983_sequence/1
type: Sequence
persistent identity: BBa M31983 sequence
display id: BBa_M31983_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gaattc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K143001_sequence/1
type: Sequence
persistent identity: BBa K143001 sequence
display id: BBa_K143001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgtttgcaaaacgattcaaaacctctttactgccgttattcgctggatttttattgctgtttcatttggttctggcaggaccggcggctgcgagtgctgaaacggcgaacaaatcgaatgagcttacagcaccgtcgatcaaaagcggaaccattcttcatgcatggaattggtcgttcaatacgttaaaacacaatatgaaggatattcatgatgcaggatatacagccattcagacatctccgattaaccaagtaaaggaagggaatcaaggagataaaagcatgtcgaactggtactggctgtatcagccgacatcgtatcaaattggcaaccgttacttaggtactgaacaagaatttaaagaaatgtgtgcagccgctgaagaatatggcataaaggtcattgttgacgcggtcatcaatcataccaccagtgattatgccgcgatttccaatgaggttaagagtattccaaactggacacatggaaacacacaaattaaaaactggtctgatcga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J70050_sequence/1
type: Sequence
persistent identity: BBa J70050 sequence
display id: BBa_J70050_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cacctgcatat
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K2027049_sequence/1
type: Sequence
persistent identity: BBa K2027049 sequence
display id: BBa_K2027049_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gaactagatcgcagcccaccggcggacagcataggacgactggctcgagcgctctactactgcggcatttctaccctgatttgtaggatcgaggtaatcc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I11073_sequence/1
type: Sequence
persistent identity: BBa I11073 sequence
display id: BBa_I11073_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: acacgatcggta
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M31997_sequence/1
type: Sequence
persistent identity: BBa M31997 sequence
display id: BBa_M31997_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ggtacc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M31953_sequence/1
type: Sequence
persistent identity: BBa M31953 sequence
display id: BBa_M31953_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ggtacc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1642006_sequence/1
type: Sequence
persistent identity: BBa K1642006 sequence
display id: BBa_K1642006_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: myers
owned by: james
elements: ccacaggtcatgaaatttatcagtacactgacccagatgggaaggtattttatgcttcctttgctgccgctgatgaccaagccacggatttaaccacggcgatcgccaatcccacggccatcgatttaattaacgccaggggatttacggcgggtagttccgtcaccgtatcgggttcctacagtcgggaagccttttttgatggatccatgggtttttatcgacttctggacgataacggtgcagtgctagatcccttaacaggtggtgtaatcaacccaggacaggtaggttatcaagaagcagctttggcagatagcaatcgtttgcaagccactggctccaccctaacggcagaagacctagaaaccagagcattttccttcaatattttgggtggcgagttgtatgcgccatttttaacggttaatgacagtctttccggtattaatcagacttattttgcctttgggtcggccaacccagatggcatcagccacagcacaaacttgggacccaacgtgattggttttgaagattttctcggcgg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K106015_sequence/1
type: Sequence
persistent identity: BBa K106015 sequence
display id: BBa_K106015_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgctcgagggagct
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_G0002_sequence/1
type: Sequence
persistent identity: BBa G0002 sequence
display id: BBa_G0002_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tactagag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M31991_sequence/1
type: Sequence
persistent identity: BBa M31991 sequence
display id: BBa_M31991_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tctaga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J70010_sequence/1
type: Sequence
persistent identity: BBa J70010 sequence
display id: BBa_J70010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gagagctggttcaccaggttaattaattaattaaacctggtgaacgtggtctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J35006_sequence/1
type: Sequence
persistent identity: BBa J35006 sequence
display id: BBa_J35006_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cgccgtgcagttttctgctctcgttttcgagtcgcatttttgcgtcgctttttagcgtggagttttctcctctggttttccagtcggcg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M31996_sequence/1
type: Sequence
persistent identity: BBa M31996 sequence
display id: BBa_M31996_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cgtacg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K259008_sequence/1
type: Sequence
persistent identity: BBa K259008 sequence
display id: BBa_K259008_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atctcaagagtggcagcggtcttgagtggcagcggcggtatacggcagcggtatgtaagtagtacaagtagcgtggggc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J56011_sequence/1
type: Sequence
persistent identity: BBa J56011 sequence
display id: BBa_J56011_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttaattaaggaaggaaactagt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K125350_sequence/1
type: Sequence
persistent identity: BBa K125350 sequence
display id: BBa_K125350_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtgaaattgtgagcggataacaatttcacacaggaaacagctatgaccatgattacggattcactggccgtcgttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcgccttgcagcacatccccctttcgccagctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacagttgcgcagcctgaatggcgaatggcgctttgcctggtttccggcaccagaagcggtgccggaaagctggctggagtgatactagaggccgccttttcctcaatcgctcttcgttcgtctggaaggcagtacaccttgataggtgggctgcccttcctggttggcttggtttcatcagccatccgcttgccctcatctgttacgccggcggtagccggccagcctcgcagagcaggattcccgttgagcaccgccaggtgcgaataagggacagtgaagaaggaacacccgctcgcgggtgggcctacttcacctatcctgcccggctgacgccgttggatacaccaaggaaagtctacacgaaccctttggcaaaatcctgtatatcgtgcgaaaaaggatggatataccgaaaaaatcgctataatgaccccgaagcagggttatgcagcggaaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K175002_sequence/1
type: Sequence
persistent identity: BBa K175002 sequence
display id: BBa_K175002_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ctctcttaaggtagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K125320_sequence/1
type: Sequence
persistent identity: BBa K125320 sequence
display id: BBa_K125320_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gaataagggacagtgaagaaggaacacccgctcgcgggtgggcctacttcacctatcctgcccggctgacgccgttggatacaccaaggaaagtctaca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K606026_sequence/1
type: Sequence
persistent identity: BBa K606026 sequence
display id: BBa_K606026_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cgtgctagaggggcggtgcttctctatcactgatagggactttcactcttctctatcactgatagggatgatcaacgttctctatcactgatagggatccccactcttgctagagtggtctcacctctctatcactgacagggacctctcttcctctctatcactgatagggacccttccctctctctatcactgatagggaccccacccactgctagagtttggctccgtctctatcactgatagggatctttcgtcatctctatcactgatagggagcctcgcctttctctatcactgatagggataatatgcattgctagagcacctgcgtttctctatcactgatagggaaccgcgactctctctatcactgatagggatcgtccccagtctctatcactgatagggactgcctccgctgctagagacccccttgctctctatcacagatagggatttcgtgttctctctatcactgatagggaccgctcaccttctctataactgatagggaacccgggccttgctagagccacttcttctcctttcactgatagggaggcgctgcattctccgtgctgtctctatcactgatagggatcccggcctttgctagagttggtatatctctctatcactgatagggacttctattcttctctatcactgatagggatggttccgcttctctatcactgatagggacaactgcttctgctagagcttcttgcggtctctatcactgatagggagatgcacagctctctatcactgatagggacgaccttgggtctctatcactgatagggacctgtcattctgctagagctccctgcattctctatcactgatagggacagcgctgagtctctatcactgatagggatctcaccttttctctatcactgatagggattgatcttcttgctagaggacccgtgtttctctatcactgatagggacctctgattctctctatcactgatagggaggctcggggttctctatcactgatagggacttgtcacgatgctagagtcgggacctgtctctatcactgatagggacccctgcctctctctatcactgatagggtctgcgtgctctctatcactgatagggataccatgacgtgctagagccctgcgcgctctctatcactgatagggatccatgctcttctctatcactgatagggattacgatccctctctatcactgatagggaccagggtttgtgctagagtgctgtagcgtctctatcactgatagggattttcctgtctctctatcactgatagggaatctcccccctctctatcactgatagggagcttctgctatgctagagtttagacccatctctatcactgatagggaacgatcgatttctctatcactgatagggacttccttgcctctctatcactgatagggagcccgcggagtgctagaggcaccccctctctctatcactgatagggagcgtgacggctctctatcactgatagggacctatattctctctatcactgataaggaccctcccagtgctagagttcctttttctctctatcactgatagggatggcccctactctctatcactgatagggagcccgtccgctctctatcactgatagggagcctcatgcttgctagagattgtctgtatctctatcactgatagggagtgttacgcctctctatcactgatagggatggcctgtgttctctatcactgatagggatccatcccactgctagagcgatgtcccctctctaccactgatagggactcatatcgtctctatcactgatagggacttatctcgtctctatcactcatagggattcccctcgatgctagaggcgcccacactctctatcactgatagggaacgtgaatgttctctatcactgatagggaaattctttagtctctatcactgatagggatccgcacttctgctagagttcaaacgtgtctctatcac…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1330000_sequence/1
type: Sequence
persistent identity: BBa K1330000 sequence
display id: BBa_K1330000_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gcccggatagctcagtcggtagagcagcggccggatgtatctgaatgaaatggtgaaggacgggtccagtaggctgcttcggcagcctacttgttgagtagagtgtgagctccgtaactagatacatccggccgcgggtccagggttcaagtccctgttcgggcgcca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_G0000_sequence/1
type: Sequence
persistent identity: BBa G0000 sequence
display id: BBa_G0000_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tactag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1930002_sequence/1
type: Sequence
persistent identity: BBa K1930002 sequence
display id: BBa_K1930002_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccggcaaaaaagggcaaggtgtcaattctcatgtttgacagcttatcatcggcaatagttacccttattatcaagataagaaagaaaaggatttttcgctacgctcaaatcctttaaaaaaacacaaaagaccacattttttaatgtggtctttattcttcaactaaagcacccattagttcaacaaacgaaaattggataaagtgggatatttttaaaatatatatttatgttacagtaatattgacttttaaaaaaggattgattctaatgaagaaagcagacaagtaagcctcctaaattcactttagataaaaatttaggaggcatatcaaatgaactttaataaaattgatttagacaattggaagagaaaagagatatttaatcattatttgaaccaacaaacgacttttagtataaccacagaaattgatattagtgttttataccgaaacataaaacaagaaggatataaattttaccctgcatttattttcttagtgacaagggtgataaactcaaatacagcttttagaactggttacaatagcgacggagagttaggttattgggataagttagagccactttatacaatttttgatggtgtatctaaaacattctctggtatttggactcctgtaaagaatgacttcaaagagttttatgatttatacctttctgatgtagagaaatataatggttcggggaaattgtttcccaaaacacctatacctgaaaatgctttttctctttctattattccatggacttcatttactgggtttaacttaaatatcaataataatagtaattaccttctacccattattacagcaggaaaattcattaataaaggtaattcaatatatttaccgctatctttacaggtacatcattctgtttgtgatggttatcatgcaggattgtttatgaactctattcaggaattgtcagataggcctaatgactggcttttataatatgagataatgccgactgtactttttacagtcggttttctaatgtcactaacctgccccgttagttgaagaaggtttttatattacagctccagatcctctacgccggacgcatcgtggccggcatcaccggcgccacaggtgcggttgctggcgcctatatcgccgacatcaccgatggggaagatcgggctcgccacttcgggctcatgagcgcttgtttcggcgtgggtatggtggcaggccccgtggccgggggactgttgggcgccatctccttgcatgcaccattccttgcggcggcggtgctcaacggcctcaacctactactgggctgcttcctaatgcaggagtcgcataagggagagcgtcgacatggatgagcgatgatgatatccgtttaggctgggcggtgatagcttctcgttcaggcagtacgcctcttttcttttccagacctgagggaggcggaaatggtgtgaggttcccggggaaaagccaaataggcgatcgcgggagtgctttatttgaagatcaggctatcactgcggtcaatagatttcacaatgtgatggctggacagcctgaggaactctcgaacccgaatggaaacaaccagatatttatgaatcagcgcggctcacatggcgttgtgctggcaaatgcaggttcatcctctgtctctatcaatacggcaacaaaattgcctgatggcaggtatgacaataaagctggagcgggttcatttcaagtgaacgatggtaaactgacaggcacgatcaatgccaggtctgtagctgtgctttatcctgatgatattgcaaaagcgcctcatgttttccttgagaattacaaaacaggtgtaacacattctttcaatgatcaactgacgattaccttgcgtgcagatgcgaatacaacaaaagccgtttatcaaatcaataatggaccagacgacaggcgtttaaggatggagatcaattcacaatcggaaaaggagatccaatttggca…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K125360_sequence/1
type: Sequence
persistent identity: BBa K125360 sequence
display id: BBa_K125360_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtgaaattgtgagcggataacaatttcacacaggaaacagctatgaccatgattacggattcactggccgtcgttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcgccttgcagcacatccccctttcgccagctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacagttgcgcagcctgaatggcgaatggcgctttgcctggtttccggcaccagaagcggtgccggaaagctggctggagtgatactagaggaataagggacagtgaagaaggaacacccgctcgcgggtgggcctacttcacctatcctgcccggctgacgccgttggatacaccaaggaaagtctaca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M31992_sequence/1
type: Sequence
persistent identity: BBa M31992 sequence
display id: BBa_M31992_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: actagt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_G0009_sequence/1
type: Sequence
persistent identity: BBa G0009 sequence
display id: BBa_G0009_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: a
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J36853_sequence/1
type: Sequence
persistent identity: BBa J36853 sequence
display id: BBa_J36853_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ggttggtgtggttggaagtagtatggaaaggattagcaatgataggcatatttatacggctcgga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1479005_sequence/1
type: Sequence
persistent identity: BBa K1479005 sequence
display id: BBa_K1479005_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aattccggaattccgg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0047_sequence/1
type: Sequence
persistent identity: BBa B0047 sequence
display id: BBa_B0047_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: caattg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I11077_sequence/1
type: Sequence
persistent identity: BBa I11077 sequence
display id: BBa_I11077_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atcgtcgacgca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M31985_sequence/1
type: Sequence
persistent identity: BBa M31985 sequence
display id: BBa_M31985_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: agatct
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J35000_sequence/1
type: Sequence
persistent identity: BBa J35000 sequence
display id: BBa_J35000_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcctcttttgaggaacaagttttcttgt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K792013_sequence/1
type: Sequence
persistent identity: BBa K792013 sequence
display id: BBa_K792013_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ggatcc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1642005_sequence/1
type: Sequence
persistent identity: BBa K1642005 sequence
display id: BBa_K1642005_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cccggtatggatggcaccgatgcggaatcccaacagattgcctttgacaacaatgtggcctggaataacctgggggatttgtccaccaccacccaacgggcctacacttcggctattagcacagacacagtgcagagtgtttatggcgttaatctggaaaaaaacgataacattcccattgtttttgcgtggcccatttttcccaccacccttaatcccacagattttcaggtaatgcttaacacgggggaaattgtcaccccggtgatcgcctctttgattcccaacagtgaatacaacgaacggcaaacggtagtaattacgggcaattttggtaatcgtttaaccccaggcacggagggagcgatttatcccgtttccgtaggcacagtgttggacagtactcctttggaaatggtgggacccaacggcccggtcagtgcggtgggtattaccattgatagtctcaacccctacgtggccggcaatggtcccaaaattgtcgccgctaagttagaccgcttcagtga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_G0003_sequence/1
type: Sequence
persistent identity: BBa G0003 sequence
display id: BBa_G0003_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gctagag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_G00100_sequence/1
type: Sequence
persistent identity: BBa G00100 sequence
display id: BBa_G00100_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tgccacctgacgtctaagaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M31961_sequence/1
type: Sequence
persistent identity: BBa M31961 sequence
display id: BBa_M31961_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cctcagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K143002_sequence/1
type: Sequence
persistent identity: BBa K143002 sequence
display id: BBa_K143002_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atccgtttaggctgggcggtgatagcttctcgttcaggcagtacgcctcttttcttttccagacctgagggaggcggaaatggtgtgaggttcccggggaaaagccaaataggcgatcgcgggagtgctttatttgaagatcaggctatcactgcggtcaatagatttcacaatgtgatggctggacagcctgaggaactctcgaacccgaatggaaacaaccagatatttatgaatcagcgcggctcacatggcgttgtgctggcaaatgcaggttcatcctctgtctctatcaatacggcaacaaaattgcctgatggcaggtatgacaataaagctggagcgggttcatttcaagtgaacgatggtaaactgacaggcacgatcaatgccaggtctgtagctgtgctttatcctgatgatattgcaaaagcgcctcatgttttccttgagaattacaaaacaggtgtaacacattctttcaatgatcaactgacgattaccttgcgtgcagatgcgaatacaacaaaagccgtttatcaaatcaataatggaccagagacggcgtttaaggatggagatcaattcacaatcggaaaaggagatccatttggcaaaacatacaccatcatgttaaaaggaacgaacagtgatggtgtaacgaggaccgagaaatacagttttgttaaaagagatccagcgtcggccaaaaccatcggctatcaaaatccgaatcattggagccaggtaaatgcttatatctataaacatgatgggagccgagtaattgaattgaccggatcttggcctggaaaaccaatgactaaaaatgcagacggaatttacacgctgacgctgcctgcggacacggatacaaccaacgcaaaagtgatttttaataatggcagcgcccaagtgcccggtcagaatcagcctggctttgattacgtgctaaatggtttatataatgactcgggcttaagcggttctcttccccattga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J35001_sequence/1
type: Sequence
persistent identity: BBa J35001 sequence
display id: BBa_J35001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gcccgcagttttctgcctcgttttcgaggggc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_G0011_sequence/1
type: Sequence
persistent identity: BBa G0011 sequence
display id: BBa_G0011_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: myers
owned by: james
elements: g
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K175001_sequence/1
type: Sequence
persistent identity: BBa K175001 sequence
display id: BBa_K175001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaaagcgatcaaagcgctgtctctggcgtctgcggcgctggttgcggcgctggttgcgggttgcgacaacaaaccggcgaccgcgccgatgccggaagttaacgacgaatcttgcaaaccggaaaacatcgcgaaaatcgaagacaaaggtgttcagcaggcgttctcttctctgtgcctgcgtcgtggtggtgacttcaaaccgtctccgaaacgtgaatggtaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J01002_sequence/1
type: Sequence
persistent identity: BBa J01002 sequence
display id: BBa_J01002_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaggctcaacaggttggtggttctcaccaccaaaagcaccacaccccacgcaaaaacaagtttttgctgatttttctttataaatagagtgttatgaaaaattagtttctcttactctctttatgatatttaaaaaagcggtgtcggcgcggctacaacaacgcgccgacaccgttttgtaggggtggtactgactatttttataaaaaacattattttatattaggggtgctgctagcggcgcggtgtgtttttttataggataccgctaggggcgctgctagcggtgcgtccctgtttgcattatgaattttagtgtttcgaaattaactttattttatgttcaaaaaaggtaatctctaatg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M34014_sequence/1
type: Sequence
persistent identity: BBa M34014 sequence
display id: BBa_M34014_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggctccgactttccaaacagctctgtttttcaccatcattagcttgagctttgcggcaccaaatgcaaaacaagtccggtggtgtgcgatatcagatcttgagcagaagaagtgcaatgatctagtgggatcctgcaatgtcccggacattacccttgtgtgtgtgcttagatcctccactgaggactgtatgacagctataaaggatggacaagcagatgccatgttcttagatagtggagaggtttatgaagcttcaaaggatccttataacctaaagccaattattgcagaaccatacagttccaatagagacttgcaaaaatgcttaaaggagcgtcaacaagctctggcaaagaagatgattggtcattatattccacaatgtgatgaaaagggcaattaccaaccacagcagtgccatggcagcactgggcactgctggtgtgttaacgcaatgggtgaaaagatttctggaacaaatactccacctggacaaacacgagctacatgtgaaaggcatgaattgccaaaatgcttgaaggaacgccaggtagctctgggaggagacgagaaagtgttaggtcgttttgttcctcagtgtgatgaaaagggcaattatgaaccacaacagttccatggcagcactgggtacagctggtgtgttaatgcaatcggtgaagagattgctggaacaaaaactccacctggaaaaataccagctacatgtcaaaaacacgatttggttactacatgtcattacgcagttgctatggtgaaaaagtccagcgcgttccagtttaatcagctgaaaggcaagaggtcctgtcactcaggtgtatcaaagacagatggctggaaagctcttgtgactgttcttgttgaaaagaagttgttgtcctgggatggacctgccaaagaatccattcaacgagccatgtcaaagttcttctcagtcagctgtatccctggagcaacccaaaccaacctctgcaagcaatgtaagggagaggaagggaagaactgtaagaacagccatgatgagccatactatggcaactatggagcattcagatgtttgaaagaagatatgggagatgtagcttttctgagaagcacagctttgtcagatgaacattcagaggtgtatgagcttctgtgtccagacaatactaggaagccccttaacaaatacaaggagtgtaacctaggaacagttccagcaggtacagtggtgaccagaaaaatcagtgacaagactgaggacatcaataactttctcatggaggctcagaaaaggcaatgcaaattgttcagttctgcacatgggaaagatttgatgtttgatgattctaccttacaattagctcttctctcatctgaagtggatgcgttcctctatctcggagtaaaattgttccatgccatgaaagcactgactggagatgcacatcttccatccaaaaataaagtgcggtggtgtacaataaataaactggaaaagatgaagtgtgatgattggtcagctgtaagcggtggggccattgcatgcacagaggcatcctgtccaaagggttgtgttaaacagattctgaaaggtgaagctgatgcagtgaaacttgaggtacaatacatgtatgaggctttgatgtgcggactgctgccagcagtagaagaataccacaataaagatgattttggtccctgtaaaacccctggatccccatacacagattttgggacactgcgtgctgtagccttggtaaaaaaaagcaacaaagacatcaactggaacaacattaaaggcaagaagtcctgccatactggtgttggtgatattgctggctgggttatccctgttagtcttattagaaggcagaatgacaattctgatattgattccttcttcggtgagagctgtgctcctggatcagatactaaatccaacctctgtaaactgtgcattggtgaccccaagaattccgcggccaatacaaa…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J70012_sequence/1
type: Sequence
persistent identity: BBa J70012 sequence
display id: BBa_J70012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aacacctggtacagcaggtggcgcgccggcgcgccagctggtgaacaccagg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K592200_sequence/1
type: Sequence
persistent identity: BBa K592200 sequence
display id: BBa_K592200_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tactagtagcggccgctgcaggagtcactaagggttagttagttagattagcagaaagtcaaaagcctccgaccggaggcttttgactaaaacttcccttggggttatcattggggctcactcaaaggcggtaatcagataaaaaaaatccttagctttcgctaaggatgatttctgctagagatggaatagactggatggaggcggataaagttgcaggaccacttctgcgctcggcccttccggctggctggtttattgctgataaatctggagccggtgagcgtgggtctcgcggtatcattgcagcactggggccagatggtaagccctcccgtatcgtagttatctacacgacggggagtcaggcaactatggatgaacgaaatagacagatcgctgagataggtgcctcactgattaagcattggtaactgtcagaccaagtttactcatatatactttagattgatttaaaacttcatttttaatttaaaaggatctaggtgaagatcctttttgataatctcatgaccaaaatcccttaacgtgagttttcgttccactgagcgtcagaccccttaataagatgatcttcttgagatcgttttggtctgcgcgtaatctcttgctctgaaaacgaaaaaaccgccttgcagggcggtttttcgaaggttctctgagctaccaactctttgaaccgaggtaactggcttggaggagcgcagtcaccaaaacttgtcctttcagtttagccttaaccggcgcatgacttcaagactaactcctctaaatcaattaccagtggctgctgccagtggtgcttttgcatgtctttccgggttggactcaagacgatagttaccggataaggcgcagcggtcggactgaacggggggttcgtgcatacagtccagcttggagcgaactgcctacccggaactgagtgtcaggcgtggaatgagacaaacgcggccataacagcggaatgacaccggtaaaccgaaaggcaggaacaggagagcgcacgagggagccgccaggggaaacgcctggtatctttatagtcctgtcgggtttcgccaccactgatttgagcgtcagatttcgtgatgcttgtcaggggggcggagcctatggaaaaacggctttgccgcggccctctcacttccctgttaagtatcttcctggcatcttccaggaaatctccgccccgttcgtaagccatttccgctcgccgcagtcgaacgaccgagcgtagcgagtcagtgagcgaggaagcggaatatatcctgtatcacatattctgctgacgcaccggtgcagccttttttctcctgccacatgaagcacttcactgacaccctcatcagtgccaacatagtaagccagtatacactccgctagcgctgaggtctgcctcgtgaagaaggtgttgctgactcataccaggcctgaatcgccccatcatccagccagaaagtgagggagccacggttgatgagagctttgttgtaggtggaccagttggtgattttgaacttttgctttgccacggaacggtctgcgttgtcgggaagatgcgtgatctgatccttcaactcagcaaaagttcgatttattcaacaaagccacgttgtgtctcaaaatctctgatgttacattgcacaagataaaaatatatcatcatgaacaataaaactgtctgcttacataaacagtaatacaaggggtgtttactagaggcgtcgaccattcatccgcttattatcacttattcaggcgtagcaaccaggcgtttaagggcaccaataactgccttaaaaaaattatttgccgactaccttggtgatctcgcctttcacgtagtggacaaattcttccaactgatctgcgcgcgaggccaagcgatcttcttcttgtccaagataagcctgtctagcttcaagtatgacgggctgatactgggccggcaggcgctccattgcccagtcggcagc…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J36854_sequence/1
type: Sequence
persistent identity: BBa J36854 sequence
display id: BBa_J36854_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ggttggtgtggttggaagtagtatggaaaggattagcaatgataggcata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_G0012_sequence/1
type: Sequence
persistent identity: BBa G0012 sequence
display id: BBa_G0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: t
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_G0010_sequence/1
type: Sequence
persistent identity: BBa G0010 sequence
display id: BBa_G0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: c
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I11074_sequence/1
type: Sequence
persistent identity: BBa I11074 sequence
display id: BBa_I11074_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: attcccgggtca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I13456_sequence/1
type: Sequence
persistent identity: BBa I13456 sequence
display id: BBa_I13456_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tattatttgtattgatctggtgaattaggtcttcgaatccgatccattgcagtgg
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z