//dna/restrictionsite
type: Collection
persistent identity: dna restrictionsite
display id: dna_restrictionsite
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
owned by: james
owned by: myers
member: BBa_I13451
member: AatII
member: NotI
member: SapI
member: NsiI
member: BbvCI
member: BclI
member: BsiWI
member: XbaI
member: I-SceI RS
member: PvuI
member: PspOMI
member: BfuAI
member: Acc651
member: SpeI
member: SacII
member: AclI
member: ApaI
member: BglII
member: SapI (reve
member: EagI
member: MluI
member: BioScaffol
member: KpnI
member: AfeI
member: AscI
member: BamHI
member: BsrG1
member: BBa_K259009
member: Acc65I
member: XmaI
member: EcoRI
member: BccI
member: NheI
member: BfaI
member: BBa_K175002
member: AseI
member: BssHII
member: BamHI rs
member: SalI
member: HpaI
member: AvrII
member: HindIII
member: MfeI
member: BBa_K259008
member: BciVI
member: BmtI

EcoRI
type: ComponentDefinition
persistent identity: BBa M31983
display id: BBa_M31983
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: EcoRI restriction enzyme site
creator: Tiffany Guo
created: 2007-02-28T12:00:00Z
modified: 2015-05-08T01:14:01Z
discontinued: false
dominant: true
experience: experience/None
group u list: _102_
m user id: 0
owner id: 1373
owning group id: 102
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: EcoRI restriction enzyme site
mutable notes: none
mutable provenance: none
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_M31983_sequence/1

MluI
type: ComponentDefinition
persistent identity: BBa I11079
display id: BBa_I11079
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: MluI restriction enzyme site with flanking nucleotides
creator: bu
created: 2004-09-06T11:00:00Z
modified: 2015-08-31T04:07:30Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2_
m user id: 0
owner id: 102
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: MluI restriction site
mutable provenance: NEB catalog
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_I11079_sequence/1

NsiI
type: ComponentDefinition
persistent identity: BBa B0046
display id: BBa_B0046
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: NsiI restriction enzyme site (PstI compatible overhang)
creator: Reshma Shetty
created: 2006-03-13T12:00:00Z
modified: 2015-08-31T04:07:20Z
discontinued: false
dominant: false
experience: experience/None
group u list: _41_6_
m user id: 0
owner id: 126
owning group id: 45
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: NsiI recognition site. Digestion with NsiI generates a compatible cohesive end to PstI.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: NsiI site
sequence: https://synbiohub.org/public/igem/BBa_B0046_sequence/1

NsiI site
type: SequenceAnnotation
persistent identity: annotation1822730
display id: annotation1822730
version: 1
top level: NsiI
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_B0046/annotation1822730/range1822730/1

https://synbiohub.org/public/igem/BBa_B0046/annotation1822730/range1822730/1
type: Range
persistent identity: range1822730
display id: range1822730
version: 1
direction: direction/forward
top level: NsiI
start: 1
end: 6
orientation: inline

BBa_I13451
type: ComponentDefinition
persistent identity: BBa I13451
display id: BBa_I13451
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Removes left Biobrick end
creator: jkm
created: 2005-05-24T11:00:00Z
modified: 2015-08-31T04:07:34Z
discontinued: true
dominant: false
experience: experience/None
group u list: _11_
m user id: 0
owner id: 253
owning group id: 6
sample status: Discontinued
status: status/Deleted
bookmark: false
mutable description: Contains an MfeI restriction site. Cut this part with MfeI+Spe and the other part with Eco+Xba. Ligation will form two mixed sites, neither of which can be recut. This effectively fixes the left side of the Biobrick part
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/DNA
role: SO:0000110

HindIII
type: ComponentDefinition
persistent identity: BBa I11076
display id: BBa_I11076
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: HindIII restriction enzyme site with extra flanking nucleotides
creator: bu
created: 2004-09-06T11:00:00Z
modified: 2015-08-31T04:07:30Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2_
m user id: 0
owner id: 102
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: HindIII restriction site with three flanking base pairs
mutable provenance: NEB catalog
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: HindIII
sequence: https://synbiohub.org/public/igem/BBa_I11076_sequence/1

HindIII
type: SequenceAnnotation
persistent identity: annotation1148736
display id: annotation1148736
version: 1
top level: HindIII
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I11076/annotation1148736/range1148736/1

https://synbiohub.org/public/igem/BBa_I11076/annotation1148736/range1148736/1
type: Range
persistent identity: range1148736
display id: range1148736
version: 1
direction: direction/forward
top level: HindIII
start: 4
end: 9
orientation: inline

SacII
type: ComponentDefinition
persistent identity: BBa I11078
display id: BBa_I11078
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: SacII restriction enzyme site with extra flanking nucleotides
creator: bu
created: 2004-09-06T11:00:00Z
modified: 2015-08-31T04:07:30Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2_
m user id: 0
owner id: 102
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: SacII restriction site
mutable provenance: NEB catalog
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_I11078_sequence/1

BsiWI
type: ComponentDefinition
persistent identity: BBa M31996
display id: BBa_M31996
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: BsiWI restriction enzyme site
creator: Tiffany Guo
created: 2007-02-27T12:00:00Z
modified: 2015-05-08T01:14:01Z
discontinued: false
dominant: false
experience: experience/None
group u list: _102_
m user id: 0
owner id: 1373
owning group id: 102
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: BsiWI restriction enzyme site
mutable notes: none
mutable provenance: A E. coli strain that carries the BsiWI gene from Bacillus species (D. Clark).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_M31996_sequence/1

HpaI
type: ComponentDefinition
persistent identity: BBa M31950
display id: BBa_M31950
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: HpaI restriction enzyme site
creator: Rebecca Adams
created: 2007-02-27T12:00:00Z
modified: 2015-05-08T01:14:01Z
discontinued: false
dominant: false
experience: experience/None
group u list: _102_
m user id: 0
owner id: 1381
owning group id: 102
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is a restriction site.
mutable notes: none
mutable provenance: standard, restriction site
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_M31950_sequence/1

MluI
type: ComponentDefinition
persistent identity: BBa M31988
display id: BBa_M31988
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: MluI restriction enzyme site
creator: Tiffany Guo
created: 2007-02-28T12:00:00Z
modified: 2015-05-08T01:14:01Z
discontinued: false
dominant: false
experience: experience/None
group u list: _102_
m user id: 0
owner id: 1373
owning group id: 102
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: MluI restriction enzyme site
mutable notes: none
mutable provenance: none
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_M31988_sequence/1

BfaI
type: ComponentDefinition
persistent identity: BBa M31967
display id: BBa_M31967
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: BfaI restriction enzyme site
creator: Rebecca Adams
created: 2007-02-27T12:00:00Z
modified: 2015-05-08T01:14:01Z
discontinued: false
dominant: false
experience: experience/None
group u list: _102_
m user id: 0
owner id: 1381
owning group id: 102
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: restriction site for BfaI
mutable notes: none
mutable provenance: known sequence
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_M31967_sequence/1

BamHI
type: ComponentDefinition
persistent identity: BBa K150008
display id: BBa_K150008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: BamHI restriction enzyme site
creator: Pascal Kraemer
created: 2008-10-20T11:00:00Z
modified: 2015-05-08T01:10:46Z
discontinued: false
dominant: true
experience: experience/None
group u list: _234_
m user id: 0
owner id: 3299
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: BamHI restriction site
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_K150008_sequence/1

SalI
type: ComponentDefinition
persistent identity: BBa I11077
display id: BBa_I11077
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: SalI restriction enzyme site with extra flanking nucleotides
creator: bu
created: 2004-09-06T11:00:00Z
modified: 2015-08-31T04:07:30Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2_
m user id: 0
owner id: 102
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: SalI restriction site
mutable provenance: NEB catalog
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_I11077_sequence/1

BBa_K259009
type: ComponentDefinition
persistent identity: BBa K259009
display id: BBa_K259009
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: BioScaffold Linker - Removes Stop Codons & scars & replaces with a Gly-Ser linker
creator: Petros Mina
created: 2009-10-12T11:00:00Z
modified: 2015-05-08T01:11:42Z
discontinued: false
dominant: false
group u list: _357_
m user id: 0
owner id: 5318
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part is part of a family of Bioscaffold - Linkers. This part will help users interested in protein-fusion. The usage of the part will allow in frame assembly of 2(+) proteins by: *Removing the stop codons from the upstream protein. *Removing the scar created by the Assembly Standard 10 (RFC10) construction of 2(+) protein coding sequences. *Maintaining the start codon of the downstream protein. *Adding a flexible (Gly-Ser-Gly) linker between the two protein coding sequences. *Acting as a translational buffer if ligation is incorrect. ===Assembly Instructions=== *Create an Assembly Standard 10 fusion as below ; **Upstream Protein Part - Bioscaffold Linker - Downstream Protein Part** *Use the BioScaffold Specific Enzymes (i)Restrict with BpuEI to remove upstream scar (created between Upstream Protein - Bioscaffold) and stop codons from upstream protein part. (ii)Ligate and you will get the intermediate product; **Upstream Protein (w/out Stop codons) - Tyr - BioScaffold Linker - Downstream Protein** *Restrict with CspCI to remove downstream scar. *Ligate to get the final product; **Upstream Protein - Tyr-Gly-Ser-Gly- Downstream Protein** ====N.B.==== CspCI cuts with a 1 b.p discrepancy depending on the sequence. However this should not affect the restriction/ligation process as this has been taken into account and incorporated into the design. Even with the discrepancy involved the restriction/ligation process will still give the same product and in-frame fusion. The other restriction variants of CspCI have also been taken into account and relevant BioScaffolds are being produced to be tested. This version is for when CspCI cuts 13/10b.p. (top/bottom) on the 5' of the recognition sequence and 10/13bp (top/bottom) on the 3' of the…
mutable notes: The CspCI discrepancy had to be taken into account
mutable provenance: This part is completely designed ''de novo''. This sequence does not occur naturally.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: Gly-Ser-Gly linker
sequence annotation: Tyr involved in restriction/ligation
sequence annotation: Translational Buffer
sequence annotation: 5' CspCI Recognition sequence
sequence annotation: BpuEI (rev. complement)
sequence annotation: BpuEI
sequence annotation: Met involved in restriction/ligation
sequence annotation: 3' CspCI recognition sequence
sequence: https://synbiohub.org/public/igem/BBa_K259009_sequence/1

Gly-Ser-Gly linker
type: SequenceAnnotation
persistent identity: annotation2037403
display id: annotation2037403
version: 1
top level: BBa_K259009
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K259009/annotation2037403/range2037403/1

https://synbiohub.org/public/igem/BBa_K259009/annotation2037403/range2037403/1
type: Range
persistent identity: range2037403
display id: range2037403
version: 1
direction: direction/forward
top level: BBa_K259009
start: 44
end: 52
orientation: inline

Tyr involved in restriction/ligation
type: SequenceAnnotation
persistent identity: annotation2037402
display id: annotation2037402
version: 1
top level: BBa_K259009
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K259009/annotation2037402/range2037402/1

https://synbiohub.org/public/igem/BBa_K259009/annotation2037402/range2037402/1
type: Range
persistent identity: range2037402
display id: range2037402
version: 1
direction: direction/forward
top level: BBa_K259009
start: 41
end: 43
orientation: inline

Translational Buffer
type: SequenceAnnotation
persistent identity: annotation2037439
display id: annotation2037439
version: 1
top level: BBa_K259009
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K259009/annotation2037439/range2037439/1

https://synbiohub.org/public/igem/BBa_K259009/annotation2037439/range2037439/1
type: Range
persistent identity: range2037439
display id: range2037439
version: 1
direction: direction/forward
top level: BBa_K259009
start: 69
end: 73
orientation: inline

5' CspCI Recognition sequence
type: SequenceAnnotation
persistent identity: annotation2037437
display id: annotation2037437
version: 1
top level: BBa_K259009
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K259009/annotation2037437/range2037437/1

https://synbiohub.org/public/igem/BBa_K259009/annotation2037437/range2037437/1
type: Range
persistent identity: range2037437
display id: range2037437
version: 1
direction: direction/forward
top level: BBa_K259009
start: 66
end: 68
orientation: inline

BpuEI (rev. complement)
type: SequenceAnnotation
persistent identity: annotation2037400
display id: annotation2037400
version: 1
top level: BBa_K259009
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K259009/annotation2037400/range2037400/1

https://synbiohub.org/public/igem/BBa_K259009/annotation2037400/range2037400/1
type: Range
persistent identity: range2037400
display id: range2037400
version: 1
direction: direction/reverse
top level: BBa_K259009
start: 3
end: 8
orientation: inline

BpuEI
type: SequenceAnnotation
persistent identity: annotation2037401
display id: annotation2037401
version: 1
top level: BBa_K259009
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K259009/annotation2037401/range2037401/1

https://synbiohub.org/public/igem/BBa_K259009/annotation2037401/range2037401/1
type: Range
persistent identity: range2037401
display id: range2037401
version: 1
direction: direction/forward
top level: BBa_K259009
start: 21
end: 26
orientation: inline

Translational Buffer
type: SequenceAnnotation
persistent identity: annotation2037405
display id: annotation2037405
version: 1
top level: BBa_K259009
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K259009/annotation2037405/range2037405/1

https://synbiohub.org/public/igem/BBa_K259009/annotation2037405/range2037405/1
type: Range
persistent identity: range2037405
display id: range2037405
version: 1
direction: direction/forward
top level: BBa_K259009
start: 56
end: 65
orientation: inline

Met involved in restriction/ligation
type: SequenceAnnotation
persistent identity: annotation2037404
display id: annotation2037404
version: 1
top level: BBa_K259009
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K259009/annotation2037404/range2037404/1

https://synbiohub.org/public/igem/BBa_K259009/annotation2037404/range2037404/1
type: Range
persistent identity: range2037404
display id: range2037404
version: 1
direction: direction/forward
top level: BBa_K259009
start: 53
end: 55
orientation: inline

3' CspCI recognition sequence
type: SequenceAnnotation
persistent identity: annotation2037438
display id: annotation2037438
version: 1
top level: BBa_K259009
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K259009/annotation2037438/range2037438/1

https://synbiohub.org/public/igem/BBa_K259009/annotation2037438/range2037438/1
type: Range
persistent identity: range2037438
display id: range2037438
version: 1
direction: direction/forward
top level: BBa_K259009
start: 74
end: 77
orientation: inline

SpeI
type: ComponentDefinition
persistent identity: BBa M31992
display id: BBa_M31992
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: SpeI restriction enzyme site
creator: Tiffany Guo
created: 2007-02-28T12:00:00Z
modified: 2015-05-08T01:14:01Z
discontinued: false
dominant: false
experience: experience/None
group u list: _102_
m user id: 0
owner id: 1373
owning group id: 102
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: SpeI restriction enzyme site
mutable notes: none
mutable provenance: none
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_M31992_sequence/1

BbvCI
type: ComponentDefinition
persistent identity: BBa M31961
display id: BBa_M31961
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: BbvCI restriction enzyme site
creator: Rebecca Adams
created: 2007-02-27T12:00:00Z
modified: 2015-05-08T01:14:01Z
discontinued: false
dominant: false
experience: experience/None
group u list: _102_
m user id: 0
owner id: 1381
owning group id: 102
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: restriction site
mutable notes: none
mutable provenance: known sequence
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_M31961_sequence/1

XmaI
type: ComponentDefinition
persistent identity: BBa I11074
display id: BBa_I11074
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: XmaI restriction enzyme site with extra flanking nucleotides
creator: bu
created: 2004-09-06T11:00:00Z
modified: 2015-08-31T04:07:30Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2_
m user id: 0
owner id: 102
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: XmaI restriction site with 3 flanking base pairs
mutable provenance: NEB catalog
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: XmaI
sequence: https://synbiohub.org/public/igem/BBa_I11074_sequence/1

XmaI
type: SequenceAnnotation
persistent identity: annotation1148485
display id: annotation1148485
version: 1
top level: XmaI
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I11074/annotation1148485/range1148485/1

https://synbiohub.org/public/igem/BBa_I11074/annotation1148485/range1148485/1
type: Range
persistent identity: range1148485
display id: range1148485
version: 1
direction: direction/forward
top level: XmaI
start: 4
end: 9
orientation: inline

XbaI
type: ComponentDefinition
persistent identity: BBa M31991
display id: BBa_M31991
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: XbaI restriction enzyme site
creator: Tiffany Guo
created: 2007-02-28T12:00:00Z
modified: 2015-05-08T01:14:01Z
discontinued: false
dominant: false
experience: experience/None
group u list: _102_
m user id: 0
owner id: 1373
owning group id: 102
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: XbaI restriction enzyme site
mutable notes: none
mutable provenance: none
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_M31991_sequence/1

I-SceI RS
type: ComponentDefinition
persistent identity: BBa K175027
display id: BBa_K175027
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: I-SceI restriction site
creator: Tim Weenink
created: 2009-07-31T11:00:00Z
modified: 2015-05-08T01:10:59Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _280_
m user id: 0
owner id: 4302
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: I-SceI homing endonuclease site Restriction site for the homing endonuclease I-SceI. Sequence will be cut as follows: 5' A G T T A C G C T A G G G A T A A^C A G G G T A A T A T A G 3' 3' T C A A T G C G A T C C C^T A T T G T C C C A T T A T A T C 5'
mutable notes: Design was very simple. The recognition site was copied from NEB and synthesised
mutable provenance: synthesised
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: I-SceI homing endonuclease restriction site
sequence: https://synbiohub.org/public/igem/BBa_K175027_sequence/1

I-SceI homing endonuclease restriction site
type: SequenceAnnotation
persistent identity: annotation2014585
display id: annotation2014585
version: 1
top level: I-SceI RS
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K175027/annotation2014585/range2014585/1

https://synbiohub.org/public/igem/BBa_K175027/annotation2014585/range2014585/1
type: Range
persistent identity: range2014585
display id: range2014585
version: 1
direction: direction/forward
top level: I-SceI RS
start: 1
end: 30
orientation: inline

XbaI
type: ComponentDefinition
persistent identity: BBa I11082
display id: BBa_I11082
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: XbaI restriction site with extra flanking nucleotides
creator: bu
created: 2004-09-06T11:00:00Z
modified: 2015-08-31T04:07:30Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2_
m user id: 0
owner id: 102
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: XbaI restriction site
mutable provenance: NEB catalogue
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_I11082_sequence/1

AfeI
type: ComponentDefinition
persistent identity: BBa M31956
display id: BBa_M31956
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: AfeI restriction enzyme site
creator: Rebecca Adams
created: 2007-02-27T12:00:00Z
modified: 2015-05-08T01:14:01Z
discontinued: false
dominant: false
experience: experience/None
group u list: _102_
m user id: 0
owner id: 1381
owning group id: 102
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Restriction site
mutable notes: none
mutable provenance: known sequence
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_M31956_sequence/1

BglII
type: ComponentDefinition
persistent identity: BBa M31985
display id: BBa_M31985
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: BglII restriction enzyme site
creator: Tiffany Guo
created: 2007-02-28T12:00:00Z
modified: 2015-05-08T01:14:01Z
discontinued: false
dominant: false
experience: experience/None
group u list: _102_
m user id: 0
owner id: 1373
owning group id: 102
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: BglII restriction enzyme site
mutable notes: none
mutable provenance: none
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_M31985_sequence/1

BioScaffol
type: ComponentDefinition
persistent identity: BBa K259002
display id: BBa_K259002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: BioScaffold Linker - Removes Stop Codons & scars & replaces with a Gly-Ser linker
creator: Petros Mina
created: 2009-07-31T11:00:00Z
modified: 2015-05-08T01:11:42Z
discontinued: false
dominant: false
experience: experience/None
group u list: _357_
m user id: 0
owner id: 5318
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part is part of a family of Bioscaffold - Linkers. This part will help users interested in protein-fusion. The usage of the part will allow in frame assembly of 2(+) proteins by: *Removing the stop codons from the upstream protein. *Removing the scar created by the Assembly Standard 10 (RFC10) construction of 2(+) protein coding sequences. *Maintaining the start codon of the downstream protein. *Adding a flexible (Gly-Ser-Gly) linker between the two protein coding sequences. *Acting as a translational buffer if ligation is incorrect. ===Assembly Instructions==== *Create an Assembly Standard 10 fusion giving ; **Upstream Protein Part - Bioscaffold Linker - Downstream Protein Part *Use the BioScaffold Specific Enzymes **Restrict with BpuEI to remove upstream scar (created between Upstream Protein - Bioscaffold) and stop codons from upstream protein part. **Ligate and you will get the intermediate product; Upstream Protein (w/out Stop codons) - Tyr - BioScaffold Linker - Downstream Protein **Restrict with BseRI to remove downstream scar. **Ligate to get the final product; Upstream Protein - Tyr-Gly-Ser-Gly- Downstream Protein
mutable notes: Part was designed taking into account RFC10 assembly standard and as an attempt to be upwards compatible with RFC10.
mutable provenance: This part was designed ''de novo''. The sequence does not occur naturally.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: Tyr Residue involved in Restriction/Ligation
sequence annotation: BpuEI Site
sequence annotation: Met involved in Restriction/Ligation
sequence annotation: BseRI Site
sequence annotation: Gly-Ser-Gly Linker
sequence annotation: Translational Buffer
sequence: https://synbiohub.org/public/igem/BBa_K259002_sequence/1

Tyr Residue involved in Restriction/Ligation
type: SequenceAnnotation
persistent identity: annotation2014589
display id: annotation2014589
version: 1
top level: BioScaffol
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K259002/annotation2014589/range2014589/1

https://synbiohub.org/public/igem/BBa_K259002/annotation2014589/range2014589/1
type: Range
persistent identity: range2014589
display id: range2014589
version: 1
direction: direction/forward
top level: BioScaffol
start: 41
end: 43
orientation: inline

BpuEI Site
type: SequenceAnnotation
persistent identity: annotation2014590
display id: annotation2014590
version: 1
top level: BioScaffol
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K259002/annotation2014590/range2014590/1

https://synbiohub.org/public/igem/BBa_K259002/annotation2014590/range2014590/1
type: Range
persistent identity: range2014590
display id: range2014590
version: 1
direction: direction/reverse
top level: BioScaffol
start: 3
end: 8
orientation: inline

BpuEI Site
type: SequenceAnnotation
persistent identity: annotation2014591
display id: annotation2014591
version: 1
top level: BioScaffol
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K259002/annotation2014591/range2014591/1

https://synbiohub.org/public/igem/BBa_K259002/annotation2014591/range2014591/1
type: Range
persistent identity: range2014591
display id: range2014591
version: 1
direction: direction/forward
top level: BioScaffol
start: 21
end: 26
orientation: inline

Met involved in Restriction/Ligation
type: SequenceAnnotation
persistent identity: annotation2014593
display id: annotation2014593
version: 1
top level: BioScaffol
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K259002/annotation2014593/range2014593/1

https://synbiohub.org/public/igem/BBa_K259002/annotation2014593/range2014593/1
type: Range
persistent identity: range2014593
display id: range2014593
version: 1
direction: direction/forward
top level: BioScaffol
start: 53
end: 55
orientation: inline

BseRI Site
type: SequenceAnnotation
persistent identity: annotation2014596
display id: annotation2014596
version: 1
top level: BioScaffol
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K259002/annotation2014596/range2014596/1

https://synbiohub.org/public/igem/BBa_K259002/annotation2014596/range2014596/1
type: Range
persistent identity: range2014596
display id: range2014596
version: 1
direction: direction/forward
top level: BioScaffol
start: 81
end: 86
orientation: inline

BseRI Site
type: SequenceAnnotation
persistent identity: annotation2014595
display id: annotation2014595
version: 1
top level: BioScaffol
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K259002/annotation2014595/range2014595/1

https://synbiohub.org/public/igem/BBa_K259002/annotation2014595/range2014595/1
type: Range
persistent identity: range2014595
display id: range2014595
version: 1
direction: direction/reverse
top level: BioScaffol
start: 63
end: 68
orientation: inline

Gly-Ser-Gly Linker
type: SequenceAnnotation
persistent identity: annotation2014592
display id: annotation2014592
version: 1
top level: BioScaffol
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K259002/annotation2014592/range2014592/1

https://synbiohub.org/public/igem/BBa_K259002/annotation2014592/range2014592/1
type: Range
persistent identity: range2014592
display id: range2014592
version: 1
direction: direction/forward
top level: BioScaffol
start: 44
end: 52
orientation: inline

Translational Buffer
type: SequenceAnnotation
persistent identity: annotation2014594
display id: annotation2014594
version: 1
top level: BioScaffol
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K259002/annotation2014594/range2014594/1

https://synbiohub.org/public/igem/BBa_K259002/annotation2014594/range2014594/1
type: Range
persistent identity: range2014594
display id: range2014594
version: 1
direction: direction/forward
top level: BioScaffol
start: 56
end: 62
orientation: inline

AatII
type: ComponentDefinition
persistent identity: BBa M31952
display id: BBa_M31952
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: AatII restriction enzyme site
creator: Rebecca Adams
created: 2007-02-27T12:00:00Z
modified: 2015-05-08T01:14:01Z
discontinued: false
dominant: false
experience: experience/None
group u list: _102_
m user id: 0
owner id: 1381
owning group id: 102
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: restriction site
mutable notes: none
mutable provenance: known it sequence
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_M31952_sequence/1

MfeI
type: ComponentDefinition
persistent identity: BBa B0047
display id: BBa_B0047
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: MfeI restriction enzyme site (EcoRI compatible overhang)
creator: Reshma Shetty
created: 2006-03-13T12:00:00Z
modified: 2015-08-31T04:07:20Z
discontinued: false
dominant: true
experience: experience/None
group u list: _41_6_
m user id: 0
owner id: 126
owning group id: 45
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: MfeI recognition site. Digestion with MfeI yields a compatible cohesive end to EcoRI.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: MfeI site
sequence: https://synbiohub.org/public/igem/BBa_B0047_sequence/1

MfeI site
type: SequenceAnnotation
persistent identity: annotation1822740
display id: annotation1822740
version: 1
top level: MfeI
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_B0047/annotation1822740/range1822740/1

https://synbiohub.org/public/igem/BBa_B0047/annotation1822740/range1822740/1
type: Range
persistent identity: range1822740
display id: range1822740
version: 1
direction: direction/forward
top level: MfeI
start: 1
end: 6
orientation: inline

BBa_K259008
type: ComponentDefinition
persistent identity: BBa K259008
display id: BBa_K259008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: BioScaffold Linker - Removes Stop Codons & scars & replaces with a Gly-Ser linker
creator: Petros Mina
created: 2009-10-12T11:00:00Z
modified: 2015-05-08T01:11:42Z
discontinued: false
dominant: false
group u list: _357_
m user id: 0
owner id: 5318
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part is part of a family of Bioscaffold - Linkers. This part will help users interested in protein-fusion. The usage of the part will allow in frame assembly of 2(+) proteins by: *Removing the stop codons from the upstream protein. *Removing the scar created by the Assembly Standard 10 (RFC10) construction of 2(+) protein coding sequences. *Maintaining the start codon of the downstream protein. *Adding a flexible (Gly-Ser-Gly) linker between the two protein coding sequences. *Acting as a translational buffer if ligation is incorrect. ===Assembly Instructions=== *Create an Assembly Standard 10 fusion as below ; **Upstream Protein Part - Bioscaffold Linker - Downstream Protein Part** *Use the BioScaffold Specific Enzymes (i)Restrict with BpuEI to remove upstream scar (created between Upstream Protein - Bioscaffold) and stop codons from upstream protein part. (ii)Ligate and you will get the intermediate product; **Upstream Protein (w/out Stop codons) - Tyr - BioScaffold Linker - Downstream Protein** *Restrict with CspCI to remove downstream scar. *Ligate to get the final product; **Upstream Protein - Tyr-Gly-Ser-Gly- Downstream Protein** ====N.B.==== CspCI cuts with a 1 b.p discrepancy depending on the sequence. However this should not affect the restriction/ligation process as this has been taken into account and incorporated into the design. Even with the discrepancy involved the restriction/ligation process will still give the same product and in-frame fusion. The other restriction variants of CspCI have also been taken into account and relevant BioScaffolds are being produced to be tested. Updates will soon follow on this. This version accounts for cleaving DNA 12/10bp (top/bottom) on the 5' of the recognition sequence and 10…
mutable notes: CspCI enzyme has different variations in the distance of restricting DNA from the recognition site.This biobrick is almost identical to part BBa_K259004 but modified to account for this discrepancy.
mutable provenance: This part is completely designed de novo. This sequence does not occur naturally.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: BpuEI (rev.comp)
sequence annotation: Translational Buffer
sequence annotation: Tyr involved in Restriction/Ligation
sequence annotation: Translational buffer
sequence annotation: 3' CspCI Recognition site
sequence annotation: 5' CspCI recognition site
sequence annotation: Gly-Ser-Gly Linker
sequence annotation: BpuEI
sequence annotation: Met involved in Restriction/Ligation
sequence: https://synbiohub.org/public/igem/BBa_K259008_sequence/1

BpuEI (rev.comp)
type: SequenceAnnotation
persistent identity: annotation2037350
display id: annotation2037350
version: 1
top level: BBa_K259008
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K259008/annotation2037350/range2037350/1

https://synbiohub.org/public/igem/BBa_K259008/annotation2037350/range2037350/1
type: Range
persistent identity: range2037350
display id: range2037350
version: 1
direction: direction/reverse
top level: BBa_K259008
start: 3
end: 8
orientation: inline

Translational Buffer
type: SequenceAnnotation
persistent identity: annotation2037355
display id: annotation2037355
version: 1
top level: BBa_K259008
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K259008/annotation2037355/range2037355/1

https://synbiohub.org/public/igem/BBa_K259008/annotation2037355/range2037355/1
type: Range
persistent identity: range2037355
display id: range2037355
version: 1
direction: direction/forward
top level: BBa_K259008
start: 56
end: 64
orientation: inline

Tyr involved in Restriction/Ligation
type: SequenceAnnotation
persistent identity: annotation2037351
display id: annotation2037351
version: 1
top level: BBa_K259008
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K259008/annotation2037351/range2037351/1

https://synbiohub.org/public/igem/BBa_K259008/annotation2037351/range2037351/1
type: Range
persistent identity: range2037351
display id: range2037351
version: 1
direction: direction/forward
top level: BBa_K259008
start: 41
end: 43
orientation: inline

Translational buffer
type: SequenceAnnotation
persistent identity: annotation2037358
display id: annotation2037358
version: 1
top level: BBa_K259008
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K259008/annotation2037358/range2037358/1

https://synbiohub.org/public/igem/BBa_K259008/annotation2037358/range2037358/1
type: Range
persistent identity: range2037358
display id: range2037358
version: 1
direction: direction/forward
top level: BBa_K259008
start: 68
end: 72
orientation: inline

3' CspCI Recognition site
type: SequenceAnnotation
persistent identity: annotation2037357
display id: annotation2037357
version: 1
top level: BBa_K259008
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K259008/annotation2037357/range2037357/1

https://synbiohub.org/public/igem/BBa_K259008/annotation2037357/range2037357/1
type: Range
persistent identity: range2037357
display id: range2037357
version: 1
direction: direction/forward
top level: BBa_K259008
start: 73
end: 76
orientation: inline

5' CspCI recognition site
type: SequenceAnnotation
persistent identity: annotation2037356
display id: annotation2037356
version: 1
top level: BBa_K259008
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K259008/annotation2037356/range2037356/1

https://synbiohub.org/public/igem/BBa_K259008/annotation2037356/range2037356/1
type: Range
persistent identity: range2037356
display id: range2037356
version: 1
direction: direction/forward
top level: BBa_K259008
start: 65
end: 67
orientation: inline

Gly-Ser-Gly Linker
type: SequenceAnnotation
persistent identity: annotation2037353
display id: annotation2037353
version: 1
top level: BBa_K259008
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K259008/annotation2037353/range2037353/1

https://synbiohub.org/public/igem/BBa_K259008/annotation2037353/range2037353/1
type: Range
persistent identity: range2037353
display id: range2037353
version: 1
direction: direction/forward
top level: BBa_K259008
start: 44
end: 52
orientation: inline

BpuEI
type: SequenceAnnotation
persistent identity: annotation2037352
display id: annotation2037352
version: 1
top level: BBa_K259008
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K259008/annotation2037352/range2037352/1

https://synbiohub.org/public/igem/BBa_K259008/annotation2037352/range2037352/1
type: Range
persistent identity: range2037352
display id: range2037352
version: 1
direction: direction/forward
top level: BBa_K259008
start: 21
end: 26
orientation: inline

Met involved in Restriction/Ligation
type: SequenceAnnotation
persistent identity: annotation2037354
display id: annotation2037354
version: 1
top level: BBa_K259008
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K259008/annotation2037354/range2037354/1

https://synbiohub.org/public/igem/BBa_K259008/annotation2037354/range2037354/1
type: Range
persistent identity: range2037354
display id: range2037354
version: 1
direction: direction/forward
top level: BBa_K259008
start: 53
end: 55
orientation: inline

BclI
type: ComponentDefinition
persistent identity: BBa M31987
display id: BBa_M31987
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: BclI restriction enzyme site
creator: Tiffany Guo
created: 2007-02-28T12:00:00Z
modified: 2015-05-08T01:14:01Z
discontinued: false
dominant: false
experience: experience/None
group u list: _102_
m user id: 0
owner id: 1373
owning group id: 102
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: BclI restriction enzyme site
mutable notes: none
mutable provenance: none
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_M31987_sequence/1

NotI
type: ComponentDefinition
persistent identity: BBa M31982
display id: BBa_M31982
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: NotI restriction enzyme site
creator: Tiffany Guo
created: 2007-02-28T12:00:00Z
modified: 2015-05-08T01:14:01Z
discontinued: false
dominant: false
experience: experience/None
group u list: _102_
m user id: 0
owner id: 1373
owning group id: 102
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: NotI restriction enzyme site
mutable notes: none
mutable provenance: none
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_M31982_sequence/1

AscI
type: ComponentDefinition
persistent identity: BBa M31990
display id: BBa_M31990
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: AscI restriction enzyme site
creator: Tiffany Guo
created: 2007-02-28T12:00:00Z
modified: 2015-05-08T01:14:01Z
discontinued: false
dominant: false
experience: experience/None
group u list: _102_
m user id: 0
owner id: 1373
owning group id: 102
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: AscI restriction enzyme site
mutable notes: none
mutable provenance: none
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_M31990_sequence/1

BBa_K175002
type: ComponentDefinition
persistent identity: BBa K175002
display id: BBa_K175002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: I-PpoI homing endonuclease site
creator: Calin Plesa
created: 2009-07-06T11:00:00Z
modified: 2015-05-08T01:10:59Z
discontinued: false
dominant: false
group u list: _280_
m user id: 0
owner id: 4286
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Restriction site for the homing endonuclease I-PpoI.
mutable notes: None
mutable provenance: Muscarella,D.E., Ellison,E.L., Ruoff,B.M. and Vogt,V.M. (1990) Characterization of I-Ppo, an intron-encoded endonuclease that mediates homing of a group I intron in the ribosomal DNA of Physarum polycephalum. Mol. Cell. Biol., 10, 3386???3396.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_K175002_sequence/1

BssHII
type: ComponentDefinition
persistent identity: BBa M31989
display id: BBa_M31989
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: BssHII restriction enzyme site
creator: Tiffany Guo
created: 2007-02-28T12:00:00Z
modified: 2015-05-08T01:14:01Z
discontinued: false
dominant: true
experience: experience/None
group u list: _102_
m user id: 0
owner id: 1373
owning group id: 102
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: BssHII restriction enzyme site
mutable notes: none
mutable provenance: none
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_M31989_sequence/1

EagI
type: ComponentDefinition
persistent identity: BBa M31986
display id: BBa_M31986
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: EagI restriction enzyme site
creator: Tiffany Guo
created: 2007-02-28T12:00:00Z
modified: 2015-05-08T01:14:01Z
discontinued: false
dominant: false
experience: experience/None
group u list: _102_
m user id: 0
owner id: 1373
owning group id: 102
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: EagI restriction enzyme site
mutable notes: none
mutable provenance: none
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_M31986_sequence/1

BamHI rs
type: ComponentDefinition
persistent identity: BBa K792013
display id: BBa_K792013
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: BamHI restriction site (standalone)
creator: Manuel Gim??nez
created: 2012-09-24T11:00:00Z
modified: 2015-05-08T01:13:23Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1047_
m user id: 0
owner id: 11811
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is a '''BamHI'''restriction site. This part might not be useful as a physical biobrick. It has been created to follow modularization in parts X
mutable notes: None
mutable provenance: None
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: BamHI restriction site
sequence: https://synbiohub.org/public/igem/BBa_K792013_sequence/1

BamHI restriction site
type: SequenceAnnotation
persistent identity: annotation2197287
display id: annotation2197287
version: 1
top level: BamHI rs
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K792013/annotation2197287/range2197287/1

https://synbiohub.org/public/igem/BBa_K792013/annotation2197287/range2197287/1
type: Range
persistent identity: range2197287
display id: range2197287
version: 1
direction: direction/forward
top level: BamHI rs
start: 1
end: 6
orientation: inline

ApaI
type: ComponentDefinition
persistent identity: BBa I11080
display id: BBa_I11080
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: ApaI restriction site with extra flanking nucleotides
creator: bu
created: 2004-09-06T11:00:00Z
modified: 2015-08-31T04:07:30Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2_
m user id: 0
owner id: 102
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: ApaI restriction site
mutable provenance: NEB catalog
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_I11080_sequence/1

NheI
type: ComponentDefinition
persistent identity: BBa B0045
display id: BBa_B0045
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: NheI restriction enzyme site (XbaI, SpeI compatible overhang)
creator: Reshma Shetty
created: 2006-03-13T12:00:00Z
modified: 2015-08-31T04:07:20Z
discontinued: false
dominant: true
experience: experience/None
group u list: _41_6_
m user id: 0
owner id: 126
owning group id: 45
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: NheI recognition site. Digestion with NheI generates a compatible cohesive end to XbaI and SpeI.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: NheI site
sequence: https://synbiohub.org/public/igem/BBa_B0045_sequence/1

NheI site
type: SequenceAnnotation
persistent identity: annotation1822724
display id: annotation1822724
version: 1
top level: NheI
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_B0045/annotation1822724/range1822724/1

https://synbiohub.org/public/igem/BBa_B0045/annotation1822724/range1822724/1
type: Range
persistent identity: range1822724
display id: range1822724
version: 1
direction: direction/forward
top level: NheI
start: 1
end: 6
orientation: inline

BfuAI
type: ComponentDefinition
persistent identity: BBa M31968
display id: BBa_M31968
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: BfuAI restriction enzyme site
creator: Rebecca Adams
created: 2007-02-27T12:00:00Z
modified: 2015-05-08T01:14:01Z
discontinued: false
dominant: false
experience: experience/None
group u list: _102_
m user id: 0
owner id: 1381
owning group id: 102
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: restriction site
mutable notes: none
mutable provenance: known sequence
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_M31968_sequence/1

PspOMI
type: ComponentDefinition
persistent identity: BBa M31984
display id: BBa_M31984
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PspOMI restriction enzyme site
creator: Tiffany Guo
created: 2007-02-28T12:00:00Z
modified: 2015-05-08T01:14:01Z
discontinued: false
dominant: false
experience: experience/None
group u list: _102_
m user id: 0
owner id: 1373
owning group id: 102
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: PspOMI restriction enzyme site
mutable notes: none
mutable provenance: none
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_M31984_sequence/1

BmtI
type: ComponentDefinition
persistent identity: BBa I11075
display id: BBa_I11075
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: BmtI restriction enzyme site with extra flanking nucleotides
creator: bu
created: 2004-09-06T11:00:00Z
modified: 2015-08-31T04:07:30Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2_
m user id: 0
owner id: 102
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: BmtI restriction site with three base pairs before site
mutable provenance: NEB catalog
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: BmtI
sequence: https://synbiohub.org/public/igem/BBa_I11075_sequence/1

BmtI
type: SequenceAnnotation
persistent identity: annotation1148641
display id: annotation1148641
version: 1
top level: BmtI
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I11075/annotation1148641/range1148641/1

https://synbiohub.org/public/igem/BBa_I11075/annotation1148641/range1148641/1
type: Range
persistent identity: range1148641
display id: range1148641
version: 1
direction: direction/forward
top level: BmtI
start: 4
end: 9
orientation: inline

AclI
type: ComponentDefinition
persistent identity: BBa I11072
display id: BBa_I11072
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: AclI restriction enzyme site with extra nucleotides
creator: bu
created: 2004-09-06T11:00:00Z
modified: 2015-08-31T04:07:30Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2_
m user id: 0
owner id: 102
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: AclI restriction site with 3 flanking bp
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: AclI
sequence: https://synbiohub.org/public/igem/BBa_I11072_sequence/1

AclI
type: SequenceAnnotation
persistent identity: annotation1148109
display id: annotation1148109
version: 1
top level: AclI
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I11072/annotation1148109/range1148109/1

https://synbiohub.org/public/igem/BBa_I11072/annotation1148109/range1148109/1
type: Range
persistent identity: range1148109
display id: range1148109
version: 1
direction: direction/forward
top level: AclI
start: 4
end: 9
orientation: inline

BciVI
type: ComponentDefinition
persistent identity: BBa M31964
display id: BBa_M31964
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: BciVI restriction enzyme site
creator: Rebecca Adams
created: 2007-02-27T12:00:00Z
modified: 2015-05-08T01:14:01Z
discontinued: false
dominant: false
experience: experience/None
group u list: _102_
m user id: 0
owner id: 1381
owning group id: 102
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: restriction site
mutable notes: none
mutable provenance: known sequence
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_M31964_sequence/1

BsrG1
type: ComponentDefinition
persistent identity: BBa M31995
display id: BBa_M31995
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: BsrGI restriction enzyme site
creator: Tiffany Guo
created: 2007-02-27T12:00:00Z
modified: 2015-05-08T01:14:01Z
discontinued: false
dominant: false
experience: experience/None
group u list: _102_
m user id: 0
owner id: 1373
owning group id: 102
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: BsrGI restriction enzyme site
mutable notes: none
mutable provenance: Bacillus stearothermophilus GR75 (Z. Chen)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_M31995_sequence/1

SapI
type: ComponentDefinition
persistent identity: BBa B0102
display id: BBa_B0102
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: SapI restriction enzyme site (offset cutter)
creator: Heather Keller
created: 2007-03-05T12:00:00Z
modified: 2015-08-31T04:07:21Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_
m user id: 0
owner id: 571
owning group id: 10
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: SapI is an offsite cutter restriction site. The recognition sequence for this enzyme is the 8 bp sequence GCTCTTCN (on the 5' strand). Cleavage at this site results in a 3 bp, 3' overhang (right side, bottom strand) directly downstream of this sequence. This specific part has a T in the 8th position.
mutable notes: This part was designed for use with Heather Keller's alternative assembly method for blunt cloning RBS sequences upstream of a coding sequence.
mutable provenance: NEB Catalog, SapI is part #R0569S.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: SapI recognition
sequence: https://synbiohub.org/public/igem/BBa_B0102_sequence/1

SapI recognition
type: SequenceAnnotation
persistent identity: annotation1920042
display id: annotation1920042
version: 1
top level: SapI
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_B0102/annotation1920042/range1920042/1

https://synbiohub.org/public/igem/BBa_B0102/annotation1920042/range1920042/1
type: Range
persistent identity: range1920042
display id: range1920042
version: 1
direction: direction/forward
top level: SapI
start: 1
end: 7
orientation: inline

PvuI
type: ComponentDefinition
persistent identity: BBa I11073
display id: BBa_I11073
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PvuI restriction enzyme site with extra flanking nucleotides
creator: bu
created: 2004-09-06T11:00:00Z
modified: 2015-08-31T04:07:30Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2_
m user id: 0
owner id: 102
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: PvuI restriction site with 3 flanking base pairs
mutable provenance: NEB catalogue
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: PvuI
sequence: https://synbiohub.org/public/igem/BBa_I11073_sequence/1

PvuI
type: SequenceAnnotation
persistent identity: annotation1148272
display id: annotation1148272
version: 1
top level: PvuI
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I11073/annotation1148272/range1148272/1

https://synbiohub.org/public/igem/BBa_I11073/annotation1148272/range1148272/1
type: Range
persistent identity: range1148272
display id: range1148272
version: 1
direction: direction/forward
top level: PvuI
start: 4
end: 9
orientation: inline

AvrII
type: ComponentDefinition
persistent identity: BBa B0048
display id: BBa_B0048
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: AvrII restriction enzyme site (XbaI, SpeI compatible overhang)
creator: Reshma Shetty
created: 2006-03-13T12:00:00Z
modified: 2015-08-31T04:07:20Z
discontinued: false
dominant: true
experience: experience/None
group u list: _41_6_
m user id: 0
owner id: 126
owning group id: 45
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: AvrII recognition site. Digestion with AvrII generates a compatible cohesive end to XbaI and SpeI.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: AvrII site
sequence: https://synbiohub.org/public/igem/BBa_B0048_sequence/1

AvrII site
type: SequenceAnnotation
persistent identity: annotation1822744
display id: annotation1822744
version: 1
top level: AvrII
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_B0048/annotation1822744/range1822744/1

https://synbiohub.org/public/igem/BBa_B0048/annotation1822744/range1822744/1
type: Range
persistent identity: range1822744
display id: range1822744
version: 1
direction: direction/forward
top level: AvrII
start: 1
end: 6
orientation: inline

ApaI
type: ComponentDefinition
persistent identity: BBa M31955
display id: BBa_M31955
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: ApaI restriction enzyme site
creator: Rebecca Adams
created: 2007-02-27T12:00:00Z
modified: 2015-05-08T01:14:01Z
discontinued: false
dominant: true
experience: experience/None
group u list: _102_
m user id: 0
owner id: 1381
owning group id: 102
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Restriction site
mutable notes: none
mutable provenance: Known cut site
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_M31955_sequence/1

BclI
type: ComponentDefinition
persistent identity: BBa M31965
display id: BBa_M31965
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: BclI restriction enzyme site
creator: Rebecca Adams
created: 2007-02-27T12:00:00Z
modified: 2015-05-08T01:14:01Z
discontinued: false
dominant: true
experience: experience/None
group u list: _102_
m user id: 0
owner id: 1381
owning group id: 102
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: restriction site
mutable notes: none
mutable provenance: known sequence
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_M31965_sequence/1

SapI (reve
type: ComponentDefinition
persistent identity: BBa B0103
display id: BBa_B0103
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: SapI restriction enzyme site, reversed (offset cutter)
creator: Heather Keller
created: 2007-03-05T12:00:00Z
modified: 2015-08-31T04:07:21Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_
m user id: 0
owner id: 571
owning group id: 10
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: SapI restriction site in the reverse orientation (recognition sequence on the 3' strand). SapI is an offsite cutter. From left to right this part includes the designed 3 bp 5' overhang (ATG), a single bp spacer, the 7 bp recognition sequence (5'-GCTCTTCT-3'), and two bp spacer. Cleavage at this site results in a 3 bp, 5' overhang (left side, top strand), which makes it compatible with Heather Keller's RBS assembly scheme for creating RBS/CDS junctions without a mixed site. This part is specific for CDSs with an ATG start site and is not compatible with GTG start sites (via HKs assembly scheme). The single bp spacer on the 3' end allows RBSes construct with this part to be used in standard biobricks assembly and is necessary to maintain the reading frame of a downstream CDS. Note that Standard Biobricks Assembly with this site results in the creation of a new ATG start site, and a 7 amino acid fusion at the N terminus of the downstream protein.
mutable notes: This part only exists in composite parts within the registry. This restriction site is used by Heather Keller for creating blunt junctions between RBS sequences and coding regions (i.e. no biobricks scar in between).
mutable provenance: NEB catalog.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: SapI
sequence annotation: 5' overhang
sequence: https://synbiohub.org/public/igem/BBa_B0103_sequence/1

SapI
type: SequenceAnnotation
persistent identity: annotation1920082
display id: annotation1920082
version: 1
top level: SapI (reve
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_B0103/annotation1920082/range1920082/1

https://synbiohub.org/public/igem/BBa_B0103/annotation1920082/range1920082/1
type: Range
persistent identity: range1920082
display id: range1920082
version: 1
direction: direction/reverse
top level: SapI (reve
start: 5
end: 11
orientation: inline

5' overhang
type: SequenceAnnotation
persistent identity: annotation1920081
display id: annotation1920081
version: 1
top level: SapI (reve
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_B0103/annotation1920081/range1920081/1

https://synbiohub.org/public/igem/BBa_B0103/annotation1920081/range1920081/1
type: Range
persistent identity: range1920081
display id: range1920081
version: 1
direction: direction/forward
top level: SapI (reve
start: 1
end: 3
orientation: inline

KpnI
type: ComponentDefinition
persistent identity: BBa I11071
display id: BBa_I11071
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: KpnI restriction enzyme site with extra flanking nucleotides
creator: bu
created: 2004-09-06T11:00:00Z
modified: 2015-08-31T04:07:30Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2_
m user id: 0
owner id: 102
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: KpnI restriction site with 3bp flanking each side
mutable provenance: NEB catalogue
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: KpnI
sequence: https://synbiohub.org/public/igem/BBa_I11071_sequence/1

KpnI
type: SequenceAnnotation
persistent identity: annotation1147967
display id: annotation1147967
version: 1
top level: KpnI
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I11071/annotation1147967/range1147967/1

https://synbiohub.org/public/igem/BBa_I11071/annotation1147967/range1147967/1
type: Range
persistent identity: range1147967
display id: range1147967
version: 1
direction: direction/forward
top level: KpnI
start: 4
end: 9
orientation: inline

AseI
type: ComponentDefinition
persistent identity: BBa M31958
display id: BBa_M31958
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: AseI restriction enzyme site
creator: Rebecca Adams
created: 2007-02-27T12:00:00Z
modified: 2015-05-08T01:14:01Z
discontinued: false
dominant: false
experience: experience/None
group u list: _102_
m user id: 0
owner id: 1381
owning group id: 102
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Restriction site
mutable notes: none
mutable provenance: known sequence
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_M31958_sequence/1

BioScaffol
type: ComponentDefinition
persistent identity: BBa K259004
display id: BBa_K259004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: BioScaffold Linker - Removes Stop Codons & scars & replaces with a Gly-Ser linker
creator: Petros Mina
created: 2009-07-31T11:00:00Z
modified: 2015-05-08T01:11:42Z
discontinued: false
dominant: false
experience: experience/None
group u list: _357_
m user id: 0
owner id: 5318
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part is part of a family of Bioscaffold - Linkers. This part will help users interested in protein-fusion. The usage of the part will allow in frame assembly of 2(+) proteins by: *Removing the stop codons from the upstream protein. *Removing the scar created by the Assembly Standard 10 (RFC10) construction of 2(+) protein coding sequences. *Maintaining the start codon of the downstream protein. *Adding a flexible (Gly-Ser-Gly) linker between the two protein coding sequences. *Acting as a translational buffer if ligation is incorrect. ===Assembly Instructions=== *Create an Assembly Standard 10 fusion as below ; **Upstream Protein Part - Bioscaffold Linker - Downstream Protein Part** *Use the BioScaffold Specific Enzymes (i)Restrict with BpuEI to remove upstream scar (created between Upstream Protein - Bioscaffold) and stop codons from upstream protein part. (ii)Ligate and you will get the intermediate product; **Upstream Protein (w/out Stop codons) - Tyr - BioScaffold Linker - Downstream Protein** *Restrict with CspCI to remove downstream scar. *Ligate to get the final product; **Upstream Protein - Tyr-Gly-Ser-Gly- Downstream Protein**
mutable notes: This part was designed taking into consideration the requirements by Assembly Standard 10 (RFC10).
mutable provenance: This part is completely designed ''de novo''. This sequence does not occur naturally.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: BpuEI
sequence annotation: Tyr Involved in Restriction/Ligation
sequence annotation: Translational Buffer
sequence annotation: CspCI (5' Recognition Seq)
sequence annotation: CspCI (3' Recognition Seq)
sequence annotation: Gly-Ser-Gly Linker
sequence annotation: Met Involved in Restriction/Ligation
sequence: https://synbiohub.org/public/igem/BBa_K259004_sequence/1

BpuEI
type: SequenceAnnotation
persistent identity: annotation2014597
display id: annotation2014597
version: 1
top level: BioScaffol
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K259004/annotation2014597/range2014597/1

https://synbiohub.org/public/igem/BBa_K259004/annotation2014597/range2014597/1
type: Range
persistent identity: range2014597
display id: range2014597
version: 1
direction: direction/reverse
top level: BioScaffol
start: 3
end: 8
orientation: inline

Tyr Involved in Restriction/Ligation
type: SequenceAnnotation
persistent identity: annotation2014599
display id: annotation2014599
version: 1
top level: BioScaffol
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K259004/annotation2014599/range2014599/1

https://synbiohub.org/public/igem/BBa_K259004/annotation2014599/range2014599/1
type: Range
persistent identity: range2014599
display id: range2014599
version: 1
direction: direction/forward
top level: BioScaffol
start: 41
end: 43
orientation: inline

Translational Buffer
type: SequenceAnnotation
persistent identity: annotation2014602
display id: annotation2014602
version: 1
top level: BioScaffol
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K259004/annotation2014602/range2014602/1

https://synbiohub.org/public/igem/BBa_K259004/annotation2014602/range2014602/1
type: Range
persistent identity: range2014602
display id: range2014602
version: 1
direction: direction/forward
top level: BioScaffol
start: 56
end: 65
orientation: inline

CspCI (5' Recognition Seq)
type: SequenceAnnotation
persistent identity: annotation2014603
display id: annotation2014603
version: 1
top level: BioScaffol
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K259004/annotation2014603/range2014603/1

https://synbiohub.org/public/igem/BBa_K259004/annotation2014603/range2014603/1
type: Range
persistent identity: range2014603
display id: range2014603
version: 1
direction: direction/forward
top level: BioScaffol
start: 66
end: 68
orientation: inline

CspCI (3' Recognition Seq)
type: SequenceAnnotation
persistent identity: annotation2014605
display id: annotation2014605
version: 1
top level: BioScaffol
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K259004/annotation2014605/range2014605/1

https://synbiohub.org/public/igem/BBa_K259004/annotation2014605/range2014605/1
type: Range
persistent identity: range2014605
display id: range2014605
version: 1
direction: direction/forward
top level: BioScaffol
start: 74
end: 77
orientation: inline

Translational Buffer
type: SequenceAnnotation
persistent identity: annotation2014604
display id: annotation2014604
version: 1
top level: BioScaffol
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K259004/annotation2014604/range2014604/1

https://synbiohub.org/public/igem/BBa_K259004/annotation2014604/range2014604/1
type: Range
persistent identity: range2014604
display id: range2014604
version: 1
direction: direction/forward
top level: BioScaffol
start: 70
end: 72
orientation: inline

BpuEI
type: SequenceAnnotation
persistent identity: annotation2014598
display id: annotation2014598
version: 1
top level: BioScaffol
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K259004/annotation2014598/range2014598/1

https://synbiohub.org/public/igem/BBa_K259004/annotation2014598/range2014598/1
type: Range
persistent identity: range2014598
display id: range2014598
version: 1
direction: direction/forward
top level: BioScaffol
start: 21
end: 26
orientation: inline

Gly-Ser-Gly Linker
type: SequenceAnnotation
persistent identity: annotation2014600
display id: annotation2014600
version: 1
top level: BioScaffol
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K259004/annotation2014600/range2014600/1

https://synbiohub.org/public/igem/BBa_K259004/annotation2014600/range2014600/1
type: Range
persistent identity: range2014600
display id: range2014600
version: 1
direction: direction/forward
top level: BioScaffol
start: 44
end: 52
orientation: inline

Met Involved in Restriction/Ligation
type: SequenceAnnotation
persistent identity: annotation2014601
display id: annotation2014601
version: 1
top level: BioScaffol
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K259004/annotation2014601/range2014601/1

https://synbiohub.org/public/igem/BBa_K259004/annotation2014601/range2014601/1
type: Range
persistent identity: range2014601
display id: range2014601
version: 1
direction: direction/forward
top level: BioScaffol
start: 53
end: 55
orientation: inline

BccI
type: ComponentDefinition
persistent identity: BBa M31962
display id: BBa_M31962
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: BccI restriction enzyme site
creator: Rebecca Adams
created: 2007-02-27T12:00:00Z
modified: 2015-05-08T01:14:01Z
discontinued: false
dominant: false
experience: experience/None
group u list: _102_
m user id: 0
owner id: 1381
owning group id: 102
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: restriction site
mutable notes: none
mutable provenance: known sequence
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_M31962_sequence/1

Acc651
type: ComponentDefinition
persistent identity: BBa M31997
display id: BBa_M31997
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Acc65I restriction enzyme site
creator: Tiffany Guo
created: 2007-02-27T12:00:00Z
modified: 2015-05-08T01:14:01Z
discontinued: false
dominant: true
experience: experience/None
group u list: _102_
m user id: 0
owner id: 1373
owning group id: 102
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Acc65I restriction enzyme site
mutable notes: none
mutable provenance: An E. coli strain that carries the cloned Acc65I gene from Acinetobacter calcoaceticus 65
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_M31997_sequence/1

Acc65I
type: ComponentDefinition
persistent identity: BBa M31953
display id: BBa_M31953
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Acc65I restriction enzyme site
creator: Rebecca Adams
created: 2007-02-27T12:00:00Z
modified: 2015-05-08T01:14:01Z
discontinued: false
dominant: true
experience: experience/None
group u list: _102_
m user id: 0
owner id: 1381
owning group id: 102
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Restriction site
mutable notes: none
mutable provenance: known sequence
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_M31953_sequence/1

BamHI
type: ComponentDefinition
persistent identity: BBa I11081
display id: BBa_I11081
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: BamHI restriction site with extra flanking nucleotides
creator: bu
created: 2004-09-06T11:00:00Z
modified: 2015-08-31T04:07:30Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2_
m user id: 0
owner id: 102
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: BamHI restriction site
mutable provenance: NEB catalogue
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_I11081_sequence/1