//function/biosynthesis/isoprenoid
type: Collection
persistent identity: function biosynthesis isoprenoid
display id: function_biosynthesis_isoprenoid
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
owned by: james
owned by: myers
member: BBa_K152005
member: rbs+crtY
member: BBa_K115050
member: BBa_K118003
member: BBa_K849001
member: BBa_K118005
member: BBa_K118002
member: BBa_K152001
member: HMG-CoA-R
member: BBa_K115057
member: BBa_K849004
member: BBa_K118006
member: BBa_K849003
member: rbs+crtIB
member: BBa_K118014
member: BBa_K152003
member: BBa_K152002
member: rbs+crtZ
member: rbs+crtE
member: BBa_K115056
member: BBa_K118013
member: ninaB
member: dxs
member: B-carotene
member: BBa_K118004
member: BBa_K118008
member: BBa_K118025

rbs+crtZ
type: ComponentDefinition
persistent identity: BBa I742158
display id: BBa_I742158
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: crtZ (beta-carotene hydroxylase) with native rbs
creator: Chris French
created: 2007-10-23T11:00:00Z
modified: 2015-08-31T04:08:03Z
discontinued: false
dominant: false
experience: experience/None
group u list: _123_
m user id: 0
owner id: 837
owning group id: 163
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: crtZ (beta-carotene hydroxylase) from Pantoea ananatis (formerly Erwinia uredovora) DSMZ 30080 (ATCC 19321). Accession: D90087. Part of the carotenoid biosynthesis pathway. Converts beta-carotene to the yellow pigment zeaxnathin.
mutable notes: This part was made as a single biobrick rather than separate rbs and coding sequence, hence the use of blunt combination to generate the final sequence.
mutable provenance: Pantoea ananatis (formerly Erwinia uredovora) DSMZ 30080 (ATCC 19321). Accession: D90087.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_I742157
component: BBa_I742156
sequence annotation: BBa_I742156
sequence annotation: BBa_I742157
sequence: https://synbiohub.org/public/igem/BBa_I742158_sequence/1

BBa_I742157
type: Component
persistent identity: component1953815
display id: component1953815
version: 1
top level: rbs+crtZ
access: public
definition: https://synbiohub.org/public/igem/BBa_I742157/1

BBa_I742156
type: Component
persistent identity: component1953813
display id: component1953813
version: 1
top level: rbs+crtZ
access: public
definition: https://synbiohub.org/public/igem/BBa_I742156/1

BBa_I742156
type: SequenceAnnotation
persistent identity: annotation1953813
display id: annotation1953813
version: 1
top level: rbs+crtZ
location: https://synbiohub.org/public/igem/BBa_I742158/annotation1953813/range1953813/1
component: BBa_I742156

https://synbiohub.org/public/igem/BBa_I742158/annotation1953813/range1953813/1
type: Range
persistent identity: range1953813
display id: range1953813
version: 1
direction: direction/reverse
top level: rbs+crtZ
start: 1
end: 16
orientation: inline

BBa_I742157
type: SequenceAnnotation
persistent identity: annotation1953815
display id: annotation1953815
version: 1
top level: rbs+crtZ
location: https://synbiohub.org/public/igem/BBa_I742158/annotation1953815/range1953815/1
component: BBa_I742157

https://synbiohub.org/public/igem/BBa_I742158/annotation1953815/range1953815/1
type: Range
persistent identity: range1953815
display id: range1953815
version: 1
direction: direction/reverse
top level: rbs+crtZ
start: 17
end: 547
orientation: inline

BBa_K118005
type: ComponentDefinition
persistent identity: BBa K118005
display id: BBa_K118005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: rbs+crtI (phytoene dehydrogenase)
creator: Andrew Hall
created: 2008-08-07T11:00:00Z
modified: 2015-05-08T01:09:36Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _192_
m user id: 0
owner id: 3282
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: This is the coding sequence of crtI from Pantoea ananatis (formerly Erwinia uredovora) (Accession number D90087) preceded by an E. coli synthetic ribosome binding site (rbs). It encodes phytoene dehydrogenase, part of the carotenoid biosynthesis pathway, which converts Phytoene to Lycopene.
mutable notes: The native crtI sequence contains two internal PstI sites. Primers crtImutf1 and crtImutf2 were ordered so that the MABEL protocol (invented for this purpose) could be used to remove these sites. The mutagenic primers were as follows: * crtImutf1: gtt tac agt aag gtt gcc * crtImutf2: agt aac tct ctg ttt gtg c In both cases, the PstI site CTGCAG was converted to CTGCAA, a silent mutation.
mutable provenance: rbs: Escherichia coli JM109 genomic DNA. crtI: Pantoea ananatis genomic DNA
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_J15001
component: BBa_K118003
sequence annotation: BBa_J15001
sequence annotation: BBa_K118003
sequence: https://synbiohub.org/public/igem/BBa_K118005_sequence/1

BBa_J15001
type: Component
persistent identity: component1970357
display id: component1970357
version: 1
top level: BBa_K118005
access: public
definition: https://synbiohub.org/public/igem/BBa_J15001/1

BBa_K118003
type: Component
persistent identity: component1970360
display id: component1970360
version: 1
top level: BBa_K118005
access: public
definition: BBa_K118003

BBa_J15001
type: SequenceAnnotation
persistent identity: annotation1970357
display id: annotation1970357
version: 1
top level: BBa_K118005
location: https://synbiohub.org/public/igem/BBa_K118005/annotation1970357/range1970357/1
component: BBa_J15001

https://synbiohub.org/public/igem/BBa_K118005/annotation1970357/range1970357/1
type: Range
persistent identity: range1970357
display id: range1970357
version: 1
direction: direction/reverse
top level: BBa_K118005
start: 1
end: 10
orientation: inline

BBa_K118003
type: SequenceAnnotation
persistent identity: annotation1970360
display id: annotation1970360
version: 1
top level: BBa_K118005
location: https://synbiohub.org/public/igem/BBa_K118005/annotation1970360/range1970360/1
component: BBa_K118003

https://synbiohub.org/public/igem/BBa_K118005/annotation1970360/range1970360/1
type: Range
persistent identity: range1970360
display id: range1970360
version: 1
direction: direction/reverse
top level: BBa_K118005
start: 17
end: 1498
orientation: inline

rbs+crtIB
type: ComponentDefinition
persistent identity: BBa I742161
display id: BBa_I742161
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: crtI (phytoene dehydrogenase) and crtB (phytoene synthase)
creator: Chris French
created: 2007-10-23T11:00:00Z
modified: 2015-08-31T04:08:03Z
discontinued: false
dominant: false
experience: experience/None
group u list: _123_
m user id: 0
owner id: 837
owning group id: 163
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: phytoene dehydrogenase and phytoene synthase from Pantoea ananatis (formerly Erwinia uredovora) DSMZ 30080 (ATCC 19321). Accession: D90087. Part of the carotenoid biosynthesis pathway. Note: the crtI coding sequence contains 2 PstI sites. In a later version of this part, these have been removed by site-directed mutagenesis but the functionality of the nutated gene has not been confirmed. This version still contains the two PstI sites. Reference: Misawa,N., Nakagawa,M., Kobayashi,K., Yamano,S., Izawa,Y., Nakamura,K. and Harashima,K. Elucidation of the Erwinia uredovora carotenoid biosynthetic pathway by functional analysis of gene products expressed in Escherichia coli. J. Bacteriol. 172 (12), 6704-6712 (1990)
mutable notes: Note: the crtI coding sequence contains 2 PstI sites. In a later version of this part, these have been removed by site-directed mutagenesis but the functionality of the nutated gene has not been confirmed. This version still contains the two PstI sites. Note also that the crtB coding sequence overlaps the crtI coding sequence; for simplicity, in this version, they have not been separated.
mutable provenance: Pantoea ananatis (formerly Erwinia uredovora) DSMZ 30080 (ATCC 19321). Accession: D90087.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_I742159
component: BBa_I742160
sequence annotation: BBa_I742160
sequence annotation: BBa_I742159
sequence: https://synbiohub.org/public/igem/BBa_I742161_sequence/1

BBa_I742159
type: Component
persistent identity: component1953826
display id: component1953826
version: 1
top level: rbs+crtIB
access: public
definition: https://synbiohub.org/public/igem/BBa_I742159/1

BBa_I742160
type: Component
persistent identity: component1953831
display id: component1953831
version: 1
top level: rbs+crtIB
access: public
definition: https://synbiohub.org/public/igem/BBa_I742160/1

BBa_I742160
type: SequenceAnnotation
persistent identity: annotation1953831
display id: annotation1953831
version: 1
top level: rbs+crtIB
location: https://synbiohub.org/public/igem/BBa_I742161/annotation1953831/range1953831/1
component: BBa_I742160

https://synbiohub.org/public/igem/BBa_I742161/annotation1953831/range1953831/1
type: Range
persistent identity: range1953831
display id: range1953831
version: 1
direction: direction/reverse
top level: rbs+crtIB
start: 23
end: 2430
orientation: inline

BBa_I742159
type: SequenceAnnotation
persistent identity: annotation1953826
display id: annotation1953826
version: 1
top level: rbs+crtIB
location: https://synbiohub.org/public/igem/BBa_I742161/annotation1953826/range1953826/1
component: BBa_I742159

https://synbiohub.org/public/igem/BBa_I742161/annotation1953826/range1953826/1
type: Range
persistent identity: range1953826
display id: range1953826
version: 1
direction: direction/reverse
top level: rbs+crtIB
start: 1
end: 22
orientation: inline

HMG-CoA-R
type: ComponentDefinition
persistent identity: BBa K849000
display id: BBa_K849000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: 3-Hydroxy-3-Methyl-Glutaryl-CoA-Reductase from Yeast
creator: Leonard Sebastian Fresenborg
created: 2012-09-19T11:00:00Z
modified: 2015-05-08T01:13:34Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1109_
m user id: 0
owner id: 12733
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: This is a truncated version of the 3-Hydroxy-3-Methyl-Glutaryl-Coenzyme-A-Reductase (HMG-CoA-R) from ''Saccharomyces cerevisiae''. It catalyses the reaction of 3-Hydroxy-3-Methyl-Glutaryle-Coenzyme-A to Mevalonate which occurs in the Mevalonate Pathway to Isopentylpyrophosphate which is the Precurser of all Isoprenoides. The enzyme is naturally a membrane bound enzyme. The membrane anchor is situated N-terminal. This region is also essential for the degradational control of the enzyme. By deleting the N-terminal part to Aminoacid (inclusive) the enzyme is expected to be water soluble and deregulated.
mutable notes: The truncation was done according to the following publication: Rico, Pardo, Orejas: 'Enhanced Production of a Plant Monoterpene by Overexpression of the 3-Hydroxy-3-Methylglutaryl Conenzyme A Reductase Catalytic Domain in Saccharomyces cerevisiae'; Applied and Enviromental Microbiology; Vol 76 p. 6449-6454; 2010
mutable provenance: The original source of this part is the genome of Saccharomyces cerevisiae. We obtained the sequence by de novo synthesis. For that we used data from the Saccharomyes Genome Database. We inserted some calm mutations to avoid conflicts with the Cloning Standard 10.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: cds
sequence: https://synbiohub.org/public/igem/BBa_K849000_sequence/1

cds
type: SequenceAnnotation
persistent identity: annotation2202427
display id: annotation2202427
version: 1
top level: HMG-CoA-R
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K849000/annotation2202427/range2202427/1

https://synbiohub.org/public/igem/BBa_K849000/annotation2202427/range2202427/1
type: Range
persistent identity: range2202427
display id: range2202427
version: 1
direction: direction/forward
top level: HMG-CoA-R
start: 1
end: 1584
orientation: inline

BBa_K118004
type: ComponentDefinition
persistent identity: BBa K118004
display id: BBa_K118004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: rbs+dxs (1-deoxyxylulose-5-phosphate synthase)
creator: Andrew Hall
created: 2008-08-07T11:00:00Z
modified: 2015-05-08T01:09:36Z
discontinued: false
dominant: false
experience: experience/None
group u list: _192_
m user id: 0
owner id: 3282
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: This is the coding sequence of dxs from Escherichia coli JM109 with added ribosome binding site (rbs). It encodes 1-deoxyxylulose-5-phosphate synthase, thiamine-requiring, which catalyses the first step in the biosynthesis of terpenoids. Overexpression of dxs has been reported to increase yields of carotenoids and other terpenoids [add reference here].
mutable notes: No special considerations.
mutable provenance: Escherichia coli JM109 genomic DNA.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_J15001
component: BBa_K118000
sequence annotation: BBa_K118000
sequence annotation: BBa_J15001
sequence: https://synbiohub.org/public/igem/BBa_K118004_sequence/1

BBa_J15001
type: Component
persistent identity: component1970353
display id: component1970353
version: 1
top level: BBa_K118004
access: public
definition: https://synbiohub.org/public/igem/BBa_J15001/1

BBa_K118000
type: Component
persistent identity: component1970354
display id: component1970354
version: 1
top level: BBa_K118004
access: public
definition: dxs

BBa_K118000
type: SequenceAnnotation
persistent identity: annotation1970354
display id: annotation1970354
version: 1
top level: BBa_K118004
location: https://synbiohub.org/public/igem/BBa_K118004/annotation1970354/range1970354/1
component: BBa_K118000

https://synbiohub.org/public/igem/BBa_K118004/annotation1970354/range1970354/1
type: Range
persistent identity: range1970354
display id: range1970354
version: 1
direction: direction/reverse
top level: BBa_K118004
start: 17
end: 1882
orientation: inline

BBa_J15001
type: SequenceAnnotation
persistent identity: annotation1970353
display id: annotation1970353
version: 1
top level: BBa_K118004
location: https://synbiohub.org/public/igem/BBa_K118004/annotation1970353/range1970353/1
component: BBa_J15001

https://synbiohub.org/public/igem/BBa_K118004/annotation1970353/range1970353/1
type: Range
persistent identity: range1970353
display id: range1970353
version: 1
direction: direction/reverse
top level: BBa_K118004
start: 1
end: 10
orientation: inline

BBa_K152005
type: ComponentDefinition
persistent identity: BBa K152005
display id: BBa_K152005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoterless Synthetic Operon of CrtE,B,I,Y and GFP
creator: David Johnston Monje
created: 2008-10-30T12:00:00Z
modified: 2015-05-08T01:10:49Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _233_
m user id: 0
owner id: 2886
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: This operon provides the full set of bacterial open reading frames necessary for beta carotene production with a GFP and termination stop signals tacked on at the end for good measure! crtE,B,I,Y coding regions from Erwinia uredovora each with a rbs preceeding the open reading frame. Likewise, the RBS-GFP-Term from biobrick BBa_E0240 is included on the end to provide a visual signal affirming transcription of preceeding 4 genes. There is no promoter on this construct, and after this GFP biobrick, no more genes can be added.
mutable notes: The synthetic operon needed a transcriptional termination signal, as well as GFP to visualize complete transcript production, so BBa_E0240 was used. No more genes can be added to this operon with this GFP on the end.
mutable provenance: This operon is made from biobricks BBa_K152001 - BBa_K152003, the biobrick BBa_K118005 which was sent to us by iGEM Edinburg, and BBa_E0240.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0010
component: BBa_B0032
component: BBa_K152003
component: BBa_K118003
component: BBa_K152002
component: BBa_B0012
component: BBa_E0040
component: BBa_K152001
component: BBa_J15001
sequence annotation: BBa_B0010
sequence annotation: BBa_K118003
sequence annotation: BBa_K152003
sequence annotation: BBa_J15001
sequence annotation: BBa_B0032
sequence annotation: BBa_K152002
sequence annotation: BBa_K152001
sequence annotation: BBa_E0040
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_K152005_sequence/1

BBa_B0010
type: Component
persistent identity: component2013168
display id: component2013168
version: 1
top level: BBa_K152005
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0032
type: Component
persistent identity: component2013164
display id: component2013164
version: 1
top level: BBa_K152005
access: public
definition: https://synbiohub.org/public/igem/BBa_B0032/1

BBa_K152003
type: Component
persistent identity: component2013162
display id: component2013162
version: 1
top level: BBa_K152005
access: public
definition: BBa_K152003

BBa_K118003
type: Component
persistent identity: component2013159
display id: component2013159
version: 1
top level: BBa_K152005
access: public
definition: BBa_K118003

BBa_K152002
type: Component
persistent identity: component2013152
display id: component2013152
version: 1
top level: BBa_K152005
access: public
definition: BBa_K152002

BBa_B0012
type: Component
persistent identity: component2013170
display id: component2013170
version: 1
top level: BBa_K152005
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_E0040
type: Component
persistent identity: component2013167
display id: component2013167
version: 1
top level: BBa_K152005
access: public
definition: https://synbiohub.org/public/igem/BBa_E0040/1

BBa_K152001
type: Component
persistent identity: component2013149
display id: component2013149
version: 1
top level: BBa_K152005
access: public
definition: BBa_K152001

BBa_J15001
type: Component
persistent identity: component2013155
display id: component2013155
version: 1
top level: BBa_K152005
access: public
definition: https://synbiohub.org/public/igem/BBa_J15001/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2013168
display id: annotation2013168
version: 1
top level: BBa_K152005
location: https://synbiohub.org/public/igem/BBa_K152005/annotation2013168/range2013168/1
component: BBa_B0010

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type: Range
persistent identity: range2013168
display id: range2013168
version: 1
direction: direction/forward
top level: BBa_K152005
start: 5284
end: 5363
orientation: inline

BBa_K118003
type: SequenceAnnotation
persistent identity: annotation2013159
display id: annotation2013159
version: 1
top level: BBa_K152005
location: https://synbiohub.org/public/igem/BBa_K152005/annotation2013159/range2013159/1
component: BBa_K118003

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type: Range
persistent identity: range2013159
display id: range2013159
version: 1
direction: direction/reverse
top level: BBa_K152005
start: 1871
end: 3352
orientation: inline

BBa_K152003
type: SequenceAnnotation
persistent identity: annotation2013162
display id: annotation2013162
version: 1
top level: BBa_K152005
location: https://synbiohub.org/public/igem/BBa_K152005/annotation2013162/range2013162/1
component: BBa_K152003

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type: Range
persistent identity: range2013162
display id: range2013162
version: 1
direction: direction/reverse
top level: BBa_K152005
start: 3361
end: 4528
orientation: inline

BBa_J15001
type: SequenceAnnotation
persistent identity: annotation2013155
display id: annotation2013155
version: 1
top level: BBa_K152005
location: https://synbiohub.org/public/igem/BBa_K152005/annotation2013155/range2013155/1
component: BBa_J15001

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type: Range
persistent identity: range2013155
display id: range2013155
version: 1
direction: direction/reverse
top level: BBa_K152005
start: 1855
end: 1864
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation2013164
display id: annotation2013164
version: 1
top level: BBa_K152005
location: https://synbiohub.org/public/igem/BBa_K152005/annotation2013164/range2013164/1
component: BBa_B0032

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type: Range
persistent identity: range2013164
display id: range2013164
version: 1
direction: direction/forward
top level: BBa_K152005
start: 4537
end: 4549
orientation: inline

BBa_K152002
type: SequenceAnnotation
persistent identity: annotation2013152
display id: annotation2013152
version: 1
top level: BBa_K152005
location: https://synbiohub.org/public/igem/BBa_K152005/annotation2013152/range2013152/1
component: BBa_K152002

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type: Range
persistent identity: range2013152
display id: range2013152
version: 1
direction: direction/reverse
top level: BBa_K152005
start: 937
end: 1846
orientation: inline

BBa_K152001
type: SequenceAnnotation
persistent identity: annotation2013149
display id: annotation2013149
version: 1
top level: BBa_K152005
location: https://synbiohub.org/public/igem/BBa_K152005/annotation2013149/range2013149/1
component: BBa_K152001

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type: Range
persistent identity: range2013149
display id: range2013149
version: 1
direction: direction/reverse
top level: BBa_K152005
start: 1
end: 928
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation2013167
display id: annotation2013167
version: 1
top level: BBa_K152005
location: https://synbiohub.org/public/igem/BBa_K152005/annotation2013167/range2013167/1
component: BBa_E0040

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type: Range
persistent identity: range2013167
display id: range2013167
version: 1
direction: direction/reverse
top level: BBa_K152005
start: 4556
end: 5275
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2013170
display id: annotation2013170
version: 1
top level: BBa_K152005
location: https://synbiohub.org/public/igem/BBa_K152005/annotation2013170/range2013170/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K152005/annotation2013170/range2013170/1
type: Range
persistent identity: range2013170
display id: range2013170
version: 1
direction: direction/forward
top level: BBa_K152005
start: 5372
end: 5412
orientation: inline

BBa_K115056
type: ComponentDefinition
persistent identity: BBa K115056
display id: BBa_K115056
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: IPP -> OPP or DMAPP -> OPP
creator: Ruud Jorna
created: 2008-07-14T11:00:00Z
modified: 2015-05-08T01:09:28Z
discontinued: false
dominant: false
experience: experience/None
group u list: _223_
m user id: 0
owner id: 3234
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This E. Coli enzyme (isopentenyl diphosphate isomerase or idi) is catalyzes the reaction from IPP (or DMAPP) to OPP, a precursor for the synthesis of FPP (farnesyl diphosphate).
mutable notes: None as of yet...
mutable provenance: E. Coli
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K115056_sequence/1

BBa_K115057
type: ComponentDefinition
persistent identity: BBa K115057
display id: BBa_K115057
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: OPP -> FPP
creator: Ruud Jorna
created: 2008-07-14T11:00:00Z
modified: 2015-05-08T01:09:28Z
discontinued: false
dominant: false
experience: experience/None
group u list: _223_
m user id: 0
owner id: 3234
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: The last enzyme required for FPP synthesis. A geranyltranstransferase from E. Coli (ACB01549).
mutable notes: None as of yet...
mutable provenance: E. Coli
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K115057_sequence/1

ninaB
type: ComponentDefinition
persistent identity: BBa K343001
display id: BBa_K343001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: B-carotene monooxygenase, Produces retinal from B-carotene. ninaB gene from Drosophila.
creator: Christian Kurtzhals
created: 2010-07-01T11:00:00Z
modified: 2015-05-08T01:12:08Z
discontinued: false
dominant: false
experience: experience/None
group u list: _465_
m user id: 0
owner id: 6083
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This coding region encodes a beta-carotene deoxygenase, that splits beta-carotene into two retinal molecules.
mutable notes: No considerations have been made so far concerning design
mutable provenance: Sequence taken from cDNA
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Coding sequence
sequence annotation: met
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_K343001_sequence/1

Coding sequence
type: SequenceAnnotation
persistent identity: annotation2072319
display id: annotation2072319
version: 1
top level: ninaB
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K343001/annotation2072319/range2072319/1

https://synbiohub.org/public/igem/BBa_K343001/annotation2072319/range2072319/1
type: Range
persistent identity: range2072319
display id: range2072319
version: 1
direction: direction/forward
top level: ninaB
start: 1
end: 1860
orientation: inline

met
type: SequenceAnnotation
persistent identity: annotation2072320
display id: annotation2072320
version: 1
top level: ninaB
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K343001/annotation2072320/range2072320/1

https://synbiohub.org/public/igem/BBa_K343001/annotation2072320/range2072320/1
type: Range
persistent identity: range2072320
display id: range2072320
version: 1
direction: direction/forward
top level: ninaB
start: 1
end: 3
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2072321
display id: annotation2072321
version: 1
top level: ninaB
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K343001/annotation2072321/range2072321/1

https://synbiohub.org/public/igem/BBa_K343001/annotation2072321/range2072321/1
type: Range
persistent identity: range2072321
display id: range2072321
version: 1
direction: direction/forward
top level: ninaB
start: 1861
end: 1863
orientation: inline

BBa_K849001
type: ComponentDefinition
persistent identity: BBa K849001
display id: BBa_K849001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Farnesyl-Pyrophosphate-Synthase from Yeast
creator: Leonard Sebastian Fresenborg
created: 2012-09-19T11:00:00Z
modified: 2015-05-08T01:13:34Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1109_
m user id: 0
owner id: 12733
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This is the Farnesyl-Pyrophosphate-Synthase from ''Saccharomyces cerevisiae'' which produces Farnesyl-Pyrophosphate from Isoprenyl Pyrophosphate and Dimethylallyl Pyrophosphate. It is encoded by the gen ERG20. We inserted some calm mutations to avoid conflicts with the Cloning Standard 10.
mutable notes: We inserted some calm mutations to avoid conflicts with the Cloning Standard 10.
mutable provenance: The source of this part is ''Saccharomyces cerevisiae''. We amplified the sequence from genomic DNA of CEN-PK-2 using the following PCR primers:
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: cds
sequence: https://synbiohub.org/public/igem/BBa_K849001_sequence/1

cds
type: SequenceAnnotation
persistent identity: annotation2202582
display id: annotation2202582
version: 1
top level: BBa_K849001
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K849001/annotation2202582/range2202582/1

https://synbiohub.org/public/igem/BBa_K849001/annotation2202582/range2202582/1
type: Range
persistent identity: range2202582
display id: range2202582
version: 1
direction: direction/forward
top level: BBa_K849001
start: 1
end: 1059
orientation: inline

BBa_K152002
type: ComponentDefinition
persistent identity: BBa K152002
display id: BBa_K152002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS + crtB
creator: David Johnston Monje
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:10:49Z
discontinued: false
dominant: false
experience: experience/None
group u list: _233_
m user id: 0
owner id: 2886
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This is the crtB gene from Erwinia uredovora with a ribosome binding site in front. This codes for the enzyme phytoene synthase and is the second step in beta-carotene production.
mutable notes: No special considerations.
mutable provenance: We got this from the plasmid pAC-3.10.10.A sent to us by Ethan Johnson of iGEM Minnesota team timebomb.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
sequence annotation: rbs
sequence annotation: cds
sequence: https://synbiohub.org/public/igem/BBa_K152002_sequence/1

rbs
type: SequenceAnnotation
persistent identity: annotation1999565
display id: annotation1999565
version: 1
top level: BBa_K152002
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K152002/annotation1999565/range1999565/1

https://synbiohub.org/public/igem/BBa_K152002/annotation1999565/range1999565/1
type: Range
persistent identity: range1999565
display id: range1999565
version: 1
direction: direction/forward
top level: BBa_K152002
start: 3
end: 10
orientation: inline

cds
type: SequenceAnnotation
persistent identity: annotation1999566
display id: annotation1999566
version: 1
top level: BBa_K152002
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K152002/annotation1999566/range1999566/1

https://synbiohub.org/public/igem/BBa_K152002/annotation1999566/range1999566/1
type: Range
persistent identity: range1999566
display id: range1999566
version: 1
direction: direction/forward
top level: BBa_K152002
start: 18
end: 908
orientation: inline

rbs+crtY
type: ComponentDefinition
persistent identity: BBa I742155
display id: BBa_I742155
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: crtY (lycopene cyclase) with native rbs
creator: Chris French
created: 2007-10-23T11:00:00Z
modified: 2015-08-31T04:08:03Z
discontinued: false
dominant: false
experience: experience/None
group u list: _123_
m user id: 0
owner id: 837
owning group id: 163
sample status: In stock
status: status/Available
bookmark: true
mutable description: crtY (lycopene cyclase) from Pantoea ananatis (formerly Erwinia uredovora) DSMZ 30080 (ATCC 19321). Accession: D90087. The native ribosome binding site is included. This is part of the carotenoid biosynthesis pathway, converting lycopene to beta-carotene.
mutable notes: This part was made as a single biobrick rather than separate rbs and coding sequence, hence the use of blunt ends in generating the final sequence.
mutable provenance: Pantoea ananatis (formerly Erwinia uredovora) DSMZ 30080 (ATCC 19321). Accession: D90087.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_I742154
component: BBa_I742153
sequence annotation: BBa_I742153
sequence annotation: BBa_I742154
sequence: https://synbiohub.org/public/igem/BBa_I742155_sequence/1

BBa_I742154
type: Component
persistent identity: component1953808
display id: component1953808
version: 1
top level: rbs+crtY
access: public
definition: https://synbiohub.org/public/igem/BBa_I742154/1

BBa_I742153
type: Component
persistent identity: component1953807
display id: component1953807
version: 1
top level: rbs+crtY
access: public
definition: https://synbiohub.org/public/igem/BBa_I742153/1

BBa_I742153
type: SequenceAnnotation
persistent identity: annotation1953807
display id: annotation1953807
version: 1
top level: rbs+crtY
location: https://synbiohub.org/public/igem/BBa_I742155/annotation1953807/range1953807/1
component: BBa_I742153

https://synbiohub.org/public/igem/BBa_I742155/annotation1953807/range1953807/1
type: Range
persistent identity: range1953807
display id: range1953807
version: 1
direction: direction/reverse
top level: rbs+crtY
start: 1
end: 19
orientation: inline

BBa_I742154
type: SequenceAnnotation
persistent identity: annotation1953808
display id: annotation1953808
version: 1
top level: rbs+crtY
location: https://synbiohub.org/public/igem/BBa_I742155/annotation1953808/range1953808/1
component: BBa_I742154

https://synbiohub.org/public/igem/BBa_I742155/annotation1953808/range1953808/1
type: Range
persistent identity: range1953808
display id: range1953808
version: 1
direction: direction/reverse
top level: rbs+crtY
start: 20
end: 1171
orientation: inline

dxs
type: ComponentDefinition
persistent identity: BBa K118000
display id: BBa_K118000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: dxs coding sequence encoding 1-deoxyxylulose-5-phosphate synthase
creator: Chris French
created: 2008-07-08T11:00:00Z
modified: 2015-05-08T01:09:36Z
discontinued: false
dominant: false
experience: experience/None
group u list: _192_
m user id: 0
owner id: 837
owning group id: 163
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: This is the coding sequence of dxs from Escherichia coli JM109. It encodes 1-deoxyxylulose-5-phosphate synthase, thiamine-requiring, which catalyses teh first step in the biosynthesis of terpenoids. Overexpression of dxs has been reported to increase yields of carotenoids and other terpenoids [add reference here].
mutable notes: No special considerations.
mutable provenance: Escherichia coli JM109 genomic DNA.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: dxs coding sequence
sequence: https://synbiohub.org/public/igem/BBa_K118000_sequence/1

dxs coding sequence
type: SequenceAnnotation
persistent identity: annotation1995699
display id: annotation1995699
version: 1
top level: dxs
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K118000/annotation1995699/range1995699/1

https://synbiohub.org/public/igem/BBa_K118000/annotation1995699/range1995699/1
type: Range
persistent identity: range1995699
display id: range1995699
version: 1
direction: direction/forward
top level: dxs
start: 1
end: 1863
orientation: inline

BBa_K118014
type: ComponentDefinition
persistent identity: BBa K118014
display id: BBa_K118014
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: rbs+crtE (geranylgeranyl pyrophosphate synthase)
creator: Andrew Hall
created: 2008-08-18T11:00:00Z
modified: 2015-05-08T01:09:37Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _192_
m user id: 0
owner id: 3282
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: This is the coding sequence of ''crtE'' from ''Pantoea ananatis'' (formerly ''Erwinia uredovora'') (Accession number D90087). It encodes geranylgeranyl pyrophosphate synthase, part of the carotenoid biosynthesis pathway, which converts farnesyl diphosphate to geranylgeranyl diphosphate.
mutable notes: The ''crtE'' BioBrick (BBa_I742151) had to be transferred from the non-compliant BABEL vector to a compliant pSB1A2 vector.
mutable provenance: The synthetic ribosome binding site was added by fusion PCR to the ''crtE'' gene, which was amplified from ''Pantoea ananatis'' DSMZ 30080 (ATCC 19321) genomic DNA.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_K118012
component: BBa_I742151
sequence annotation: BBa_I742151
sequence annotation: BBa_K118012
sequence: https://synbiohub.org/public/igem/BBa_K118014_sequence/1

BBa_K118012
type: Component
persistent identity: component1972439
display id: component1972439
version: 1
top level: BBa_K118014
access: public
definition: https://synbiohub.org/public/igem/BBa_K118012/1

BBa_I742151
type: Component
persistent identity: component1972440
display id: component1972440
version: 1
top level: BBa_K118014
access: public
definition: https://synbiohub.org/public/igem/BBa_I742151/1

BBa_I742151
type: SequenceAnnotation
persistent identity: annotation1972440
display id: annotation1972440
version: 1
top level: BBa_K118014
location: https://synbiohub.org/public/igem/BBa_K118014/annotation1972440/range1972440/1
component: BBa_I742151

https://synbiohub.org/public/igem/BBa_K118014/annotation1972440/range1972440/1
type: Range
persistent identity: range1972440
display id: range1972440
version: 1
direction: direction/reverse
top level: BBa_K118014
start: 11
end: 922
orientation: inline

BBa_K118012
type: SequenceAnnotation
persistent identity: annotation1972439
display id: annotation1972439
version: 1
top level: BBa_K118014
location: https://synbiohub.org/public/igem/BBa_K118014/annotation1972439/range1972439/1
component: BBa_K118012

https://synbiohub.org/public/igem/BBa_K118014/annotation1972439/range1972439/1
type: Range
persistent identity: range1972439
display id: range1972439
version: 1
direction: direction/reverse
top level: BBa_K118014
start: 1
end: 4
orientation: inline

B-carotene
type: ComponentDefinition
persistent identity: BBa K343006
display id: BBa_K343006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Expresses B-carotene monooxygenase on a constitutive promotor
creator: Christian Kurtzhals
created: 2010-10-13T11:00:00Z
modified: 2015-05-08T01:12:08Z
discontinued: false
dominant: false
experience: experience/None
group u list: _465_
m user id: 0
owner id: 6083
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This generator expresses a beta-carotene 15,15'-monooxygenase,on a constitutive promotor, that catalyses retinal production from beta-carotene via the following reaction: beta-carotene + O2 = 2 retinal
mutable notes: The brick requires B-carotene supply to function. This can either be supplied externally, or alternatively the BBa_K274210 B-carotene generator will induce full B-carotene synthesis in E. coli.
mutable provenance: The ninaB gene was originally cloned from drosophila melanogaster, and cDNA was aquired for production of the biobrick from the Drosophila Genome Ressource Center. Promotor+rbs brick BBa_J13002 and terminator BBa_B0015 were used.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0034
component: BBa_B0010
component: BBa_R0040
component: BBa_K343001
component: BBa_B0012
sequence annotation: BBa_R0040
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_B0034
sequence annotation: BBa_K343001
sequence: https://synbiohub.org/public/igem/BBa_K343006_sequence/1

BBa_B0034
type: Component
persistent identity: component2085984
display id: component2085984
version: 1
top level: B-carotene
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0010
type: Component
persistent identity: component2085989
display id: component2085989
version: 1
top level: B-carotene
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_R0040
type: Component
persistent identity: component2085978
display id: component2085978
version: 1
top level: B-carotene
access: public
definition: https://synbiohub.org/public/igem/BBa_R0040/1

BBa_K343001
type: Component
persistent identity: component2085988
display id: component2085988
version: 1
top level: B-carotene
access: public
definition: ninaB

BBa_B0012
type: Component
persistent identity: component2085991
display id: component2085991
version: 1
top level: B-carotene
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation2085978
display id: annotation2085978
version: 1
top level: B-carotene
location: https://synbiohub.org/public/igem/BBa_K343006/annotation2085978/range2085978/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_K343006/annotation2085978/range2085978/1
type: Range
persistent identity: range2085978
display id: range2085978
version: 1
direction: direction/forward
top level: B-carotene
start: 1
end: 54
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2085989
display id: annotation2085989
version: 1
top level: B-carotene
location: https://synbiohub.org/public/igem/BBa_K343006/annotation2085989/range2085989/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K343006/annotation2085989/range2085989/1
type: Range
persistent identity: range2085989
display id: range2085989
version: 1
direction: direction/forward
top level: B-carotene
start: 1952
end: 2031
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2085991
display id: annotation2085991
version: 1
top level: B-carotene
location: https://synbiohub.org/public/igem/BBa_K343006/annotation2085991/range2085991/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K343006/annotation2085991/range2085991/1
type: Range
persistent identity: range2085991
display id: range2085991
version: 1
direction: direction/forward
top level: B-carotene
start: 2040
end: 2080
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2085984
display id: annotation2085984
version: 1
top level: B-carotene
location: https://synbiohub.org/public/igem/BBa_K343006/annotation2085984/range2085984/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K343006/annotation2085984/range2085984/1
type: Range
persistent identity: range2085984
display id: range2085984
version: 1
direction: direction/reverse
top level: B-carotene
start: 63
end: 74
orientation: inline

BBa_K343001
type: SequenceAnnotation
persistent identity: annotation2085988
display id: annotation2085988
version: 1
top level: B-carotene
location: https://synbiohub.org/public/igem/BBa_K343006/annotation2085988/range2085988/1
component: BBa_K343001

https://synbiohub.org/public/igem/BBa_K343006/annotation2085988/range2085988/1
type: Range
persistent identity: range2085988
display id: range2085988
version: 1
direction: direction/reverse
top level: B-carotene
start: 81
end: 1943
orientation: inline

BBa_K115050
type: ComponentDefinition
persistent identity: BBa K115050
display id: BBa_K115050
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: A-coA -> AA-coA
creator: Ruud Jorna
created: 2008-07-14T11:00:00Z
modified: 2015-05-08T01:09:28Z
discontinued: false
dominant: false
experience: experience/None
group u list: _223_
m user id: 0
owner id: 3234
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This is the first enzyme in the mevalonate pathway we want to introduce into E. coli. It is needed to turn acetyl-coA into acetoacetyl-coA. The enzyme itself originates from E. coli and is called AtoB (AE014075). An alternative to this would be ERG10 from S. Cerevisiae.
mutable notes: None as of yet...
mutable provenance: Escherichia Coli
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K115050_sequence/1

rbs+crtE
type: ComponentDefinition
persistent identity: BBa I742152
display id: BBa_I742152
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: crtE (geranylgeranyl pyrophosphate synthase) with native rbs.
creator: Chris French
created: 2007-10-23T11:00:00Z
modified: 2015-08-31T04:08:03Z
discontinued: false
dominant: false
experience: experience/None
group u list: _123_
m user id: 0
owner id: 837
owning group id: 163
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: crtE (geranylgeranyl pyrophosphate synthase) from Pantoea ananatis (formerly Erwinia uredovora) DSMZ 30080 (ATCC 19321). Accession: D90087. The native ribosome bindind site is included. This gene is part of the carotenoid biosynthesis pathway.
mutable notes: This part was made as a single biobrick rather than separate rbs and coding sequence, hence the use of blunt ligation in generating the final sequence.
mutable provenance: Pantoea ananatis (formerly Erwinia uredovora) DSMZ 30080 (ATCC 19321). Accession: D90087.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_I742151
component: BBa_I742150
sequence annotation: BBa_I742150
sequence annotation: BBa_I742151
sequence: https://synbiohub.org/public/igem/BBa_I742152_sequence/1

BBa_I742151
type: Component
persistent identity: component1953806
display id: component1953806
version: 1
top level: rbs+crtE
access: public
definition: https://synbiohub.org/public/igem/BBa_I742151/1

BBa_I742150
type: Component
persistent identity: component1953805
display id: component1953805
version: 1
top level: rbs+crtE
access: public
definition: https://synbiohub.org/public/igem/BBa_I742150/1

BBa_I742150
type: SequenceAnnotation
persistent identity: annotation1953805
display id: annotation1953805
version: 1
top level: rbs+crtE
location: https://synbiohub.org/public/igem/BBa_I742152/annotation1953805/range1953805/1
component: BBa_I742150

https://synbiohub.org/public/igem/BBa_I742152/annotation1953805/range1953805/1
type: Range
persistent identity: range1953805
display id: range1953805
version: 1
direction: direction/reverse
top level: rbs+crtE
start: 1
end: 42
orientation: inline

BBa_I742151
type: SequenceAnnotation
persistent identity: annotation1953806
display id: annotation1953806
version: 1
top level: rbs+crtE
location: https://synbiohub.org/public/igem/BBa_I742152/annotation1953806/range1953806/1
component: BBa_I742151

https://synbiohub.org/public/igem/BBa_I742152/annotation1953806/range1953806/1
type: Range
persistent identity: range1953806
display id: range1953806
version: 1
direction: direction/reverse
top level: rbs+crtE
start: 43
end: 954
orientation: inline

BBa_K118025
type: ComponentDefinition
persistent identity: BBa K118025
display id: BBa_K118025
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: limonene generator with lac promoter
creator: Chris French
created: 2008-10-22T11:00:00Z
modified: 2015-05-08T01:09:37Z
discontinued: false
dominant: false
experience: experience/None
group u list: _192_
m user id: 0
owner id: 837
owning group id: 163
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: This part consists of a lac promoter (with lacZ' tag for blue-white selection) coupled to the limonene generation system consisting of dxs (deoxyxylulose phosphate synthase, rate controlling step of terpenoid biosynthesis in bacteria) + LIMS1 (limonene synthase 1 from lemon) + appY (a transcription factor known to increase terpenoid biosynthesis).
mutable notes: No special considerations.
mutable provenance: The lac promoter, dxs and appY are from E. coli; LIMS1 is from lemon.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_J15001
component: BBa_K118001
component: BBa_I742110
component: BBa_J33207
component: BBa_K118000
sequence annotation: BBa_J15001
sequence annotation: BBa_I742110
sequence annotation: BBa_J33207
sequence annotation: BBa_K118000
sequence annotation: BBa_K118001
sequence: https://synbiohub.org/public/igem/BBa_K118025_sequence/1

BBa_J15001
type: Component
persistent identity: component1985162
display id: component1985162
version: 1
top level: BBa_K118025
access: public
definition: https://synbiohub.org/public/igem/BBa_J15001/1

BBa_J15001
type: Component
persistent identity: component1985166
display id: component1985166
version: 1
top level: BBa_K118025
access: public
definition: https://synbiohub.org/public/igem/BBa_J15001/1

BBa_K118001
type: Component
persistent identity: component1985174
display id: component1985174
version: 1
top level: BBa_K118025
access: public
definition: https://synbiohub.org/public/igem/BBa_K118001/1

BBa_I742110
type: Component
persistent identity: component1985170
display id: component1985170
version: 1
top level: BBa_K118025
access: public
definition: https://synbiohub.org/public/igem/BBa_I742110/1

BBa_J33207
type: Component
persistent identity: component1985159
display id: component1985159
version: 1
top level: BBa_K118025
access: public
definition: https://synbiohub.org/public/igem/BBa_J33207/1

BBa_J15001
type: Component
persistent identity: component1985173
display id: component1985173
version: 1
top level: BBa_K118025
access: public
definition: https://synbiohub.org/public/igem/BBa_J15001/1

BBa_K118000
type: Component
persistent identity: component1985163
display id: component1985163
version: 1
top level: BBa_K118025
access: public
definition: dxs

BBa_J15001
type: SequenceAnnotation
persistent identity: annotation1985166
display id: annotation1985166
version: 1
top level: BBa_K118025
location: https://synbiohub.org/public/igem/BBa_K118025/annotation1985166/range1985166/1
component: BBa_J15001

https://synbiohub.org/public/igem/BBa_K118025/annotation1985166/range1985166/1
type: Range
persistent identity: range1985166
display id: range1985166
version: 1
direction: direction/reverse
top level: BBa_K118025
start: 2499
end: 2508
orientation: inline

BBa_I742110
type: SequenceAnnotation
persistent identity: annotation1985170
display id: annotation1985170
version: 1
top level: BBa_K118025
location: https://synbiohub.org/public/igem/BBa_K118025/annotation1985170/range1985170/1
component: BBa_I742110

https://synbiohub.org/public/igem/BBa_K118025/annotation1985170/range1985170/1
type: Range
persistent identity: range1985170
display id: range1985170
version: 1
direction: direction/reverse
top level: BBa_K118025
start: 2515
end: 4185
orientation: inline

BBa_J15001
type: SequenceAnnotation
persistent identity: annotation1985162
display id: annotation1985162
version: 1
top level: BBa_K118025
location: https://synbiohub.org/public/igem/BBa_K118025/annotation1985162/range1985162/1
component: BBa_J15001

https://synbiohub.org/public/igem/BBa_K118025/annotation1985162/range1985162/1
type: Range
persistent identity: range1985162
display id: range1985162
version: 1
direction: direction/reverse
top level: BBa_K118025
start: 609
end: 618
orientation: inline

BBa_J33207
type: SequenceAnnotation
persistent identity: annotation1985159
display id: annotation1985159
version: 1
top level: BBa_K118025
location: https://synbiohub.org/public/igem/BBa_K118025/annotation1985159/range1985159/1
component: BBa_J33207

https://synbiohub.org/public/igem/BBa_K118025/annotation1985159/range1985159/1
type: Range
persistent identity: range1985159
display id: range1985159
version: 1
direction: direction/reverse
top level: BBa_K118025
start: 1
end: 600
orientation: inline

BBa_K118000
type: SequenceAnnotation
persistent identity: annotation1985163
display id: annotation1985163
version: 1
top level: BBa_K118025
location: https://synbiohub.org/public/igem/BBa_K118025/annotation1985163/range1985163/1
component: BBa_K118000

https://synbiohub.org/public/igem/BBa_K118025/annotation1985163/range1985163/1
type: Range
persistent identity: range1985163
display id: range1985163
version: 1
direction: direction/reverse
top level: BBa_K118025
start: 625
end: 2490
orientation: inline

BBa_K118001
type: SequenceAnnotation
persistent identity: annotation1985174
display id: annotation1985174
version: 1
top level: BBa_K118025
location: https://synbiohub.org/public/igem/BBa_K118025/annotation1985174/range1985174/1
component: BBa_K118001

https://synbiohub.org/public/igem/BBa_K118025/annotation1985174/range1985174/1
type: Range
persistent identity: range1985174
display id: range1985174
version: 1
direction: direction/reverse
top level: BBa_K118025
start: 4210
end: 4962
orientation: inline

BBa_J15001
type: SequenceAnnotation
persistent identity: annotation1985173
display id: annotation1985173
version: 1
top level: BBa_K118025
location: https://synbiohub.org/public/igem/BBa_K118025/annotation1985173/range1985173/1
component: BBa_J15001

https://synbiohub.org/public/igem/BBa_K118025/annotation1985173/range1985173/1
type: Range
persistent identity: range1985173
display id: range1985173
version: 1
direction: direction/reverse
top level: BBa_K118025
start: 4194
end: 4203
orientation: inline

BBa_K849004
type: ComponentDefinition
persistent identity: BBa K849004
display id: BBa_K849004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: ent-Kaurene Oxidase from Gibberella fujikuroi
creator: Martin Schneider
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:34Z
discontinued: false
dominant: false
group u list: _1109_
m user id: 0
owner id: 12313
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: KO
mutable notes: no
mutable provenance: Genomic Sequence from Gibberella fujikuroi
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: cds
sequence: https://synbiohub.org/public/igem/BBa_K849004_sequence/1

cds
type: SequenceAnnotation
persistent identity: annotation2202568
display id: annotation2202568
version: 1
top level: BBa_K849004
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K849004/annotation2202568/range2202568/1

https://synbiohub.org/public/igem/BBa_K849004/annotation2202568/range2202568/1
type: Range
persistent identity: range2202568
display id: range2202568
version: 1
direction: direction/forward
top level: BBa_K849004
start: 1
end: 1578
orientation: inline

BBa_K118002
type: ComponentDefinition
persistent identity: BBa K118002
display id: BBa_K118002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: crtB coding sequence encoding phytoene synthase
creator: Andrew Hall
created: 2008-07-23T11:00:00Z
modified: 2015-05-08T01:09:36Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _192_
m user id: 0
owner id: 3282
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: This is the coding sequence of crtB from Pantoea ananatis (formerly Erwinia uredovora) (Accession number D90087). It encodes phytoene synthase, part of the carotenoid biosynthesis pathway, which converts geranylgeranyl diphosphate to phytoene. Reference: Misawa,N., Nakagawa,M., Kobayashi,K., Yamano,S., Izawa,Y., Nakamura,K. and Harashima,K. Elucidation of the Erwinia uredovora carotenoid biosynthetic pathway by functional analysis of gene products expressed in Escherichia coli. J. Bacteriol. 172 (12), 6704-6712 (1990)
mutable notes: No special considerations.
mutable provenance: Pantoea ananatis genomic DNA
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: crtB coding sequence
sequence: https://synbiohub.org/public/igem/BBa_K118002_sequence/1

crtB coding sequence
type: SequenceAnnotation
persistent identity: annotation1995701
display id: annotation1995701
version: 1
top level: BBa_K118002
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K118002/annotation1995701/range1995701/1

https://synbiohub.org/public/igem/BBa_K118002/annotation1995701/range1995701/1
type: Range
persistent identity: range1995701
display id: range1995701
version: 1
direction: direction/forward
top level: BBa_K118002
start: 1
end: 930
orientation: inline

BBa_K118013
type: ComponentDefinition
persistent identity: BBa K118013
display id: BBa_K118013
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: rbs+crtY (lycopene B-cyclase)
creator: Andrew Hall
created: 2008-08-18T11:00:00Z
modified: 2015-05-08T01:09:36Z
discontinued: false
dominant: false
experience: experience/None
group u list: _192_
m user id: 0
owner id: 3282
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: This is the coding sequence of ''crtY'' from ''Pantoea ananatis'' (formerly ''Erwinia uredovora'') (Accession number D90087). It encodes lycopene B-cyclase, part of the carotenoid biosynthesis pathway, which converts lycopene to B-carotene.
mutable notes: No special considerations
mutable provenance: The synthetic ribosome binding site was added by fusion PCR to the ''crtY'' gene, which was amplified from ''P. ananatis'' genomic DNA.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_K118008
component: BBa_K118012
sequence annotation: BBa_K118012
sequence annotation: BBa_K118008
sequence: https://synbiohub.org/public/igem/BBa_K118013_sequence/1

BBa_K118008
type: Component
persistent identity: component1972438
display id: component1972438
version: 1
top level: BBa_K118013
access: public
definition: BBa_K118008

BBa_K118012
type: Component
persistent identity: component1972437
display id: component1972437
version: 1
top level: BBa_K118013
access: public
definition: https://synbiohub.org/public/igem/BBa_K118012/1

BBa_K118012
type: SequenceAnnotation
persistent identity: annotation1972437
display id: annotation1972437
version: 1
top level: BBa_K118013
location: https://synbiohub.org/public/igem/BBa_K118013/annotation1972437/range1972437/1
component: BBa_K118012

https://synbiohub.org/public/igem/BBa_K118013/annotation1972437/range1972437/1
type: Range
persistent identity: range1972437
display id: range1972437
version: 1
direction: direction/reverse
top level: BBa_K118013
start: 1
end: 4
orientation: inline

BBa_K118008
type: SequenceAnnotation
persistent identity: annotation1972438
display id: annotation1972438
version: 1
top level: BBa_K118013
location: https://synbiohub.org/public/igem/BBa_K118013/annotation1972438/range1972438/1
component: BBa_K118008

https://synbiohub.org/public/igem/BBa_K118013/annotation1972438/range1972438/1
type: Range
persistent identity: range1972438
display id: range1972438
version: 1
direction: direction/reverse
top level: BBa_K118013
start: 11
end: 1162
orientation: inline

BBa_K118008
type: ComponentDefinition
persistent identity: BBa K118008
display id: BBa_K118008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: crtY coding sequence encoding lycopene B-cyclase
creator: Andrew Hall
created: 2008-08-18T11:00:00Z
modified: 2015-05-08T01:09:36Z
discontinued: false
dominant: false
experience: experience/None
group u list: _192_
m user id: 0
owner id: 3282
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: This is the coding sequence of crtY from Pantoea ananatis (formerly Erwinia uredovora) (Accession number D90087). It encodes lycopene B-cyclase, part of the carotenoid biosynthesis pathway, which converts lycopene to B-carotene.
mutable notes: No special considerations
mutable provenance: Pantoea ananatis genomic DNA
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: crtY coding sequence
sequence: https://synbiohub.org/public/igem/BBa_K118008_sequence/1

crtY coding sequence
type: SequenceAnnotation
persistent identity: annotation1995716
display id: annotation1995716
version: 1
top level: BBa_K118008
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K118008/annotation1995716/range1995716/1

https://synbiohub.org/public/igem/BBa_K118008/annotation1995716/range1995716/1
type: Range
persistent identity: range1995716
display id: range1995716
version: 1
direction: direction/forward
top level: BBa_K118008
start: 1
end: 1149
orientation: inline

BBa_K152003
type: ComponentDefinition
persistent identity: BBa K152003
display id: BBa_K152003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CrtY + RBS
creator: David Johnston Monje
created: 2008-10-30T12:00:00Z
modified: 2015-05-08T01:10:49Z
discontinued: false
dominant: false
experience: experience/None
group u list: _233_
m user id: 0
owner id: 2886
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: crtE from Erwinia uredovora with a rbs preceeding it. This gene codes for lycopene cyclase which is the third step in the beta carotene synthesis pathway. In combination with CrtE and B, this works to produce lycopene, a red, antioxidant, carotenoid.
mutable notes: We needed to use site directed mutagenesis to add the SpeI site after the stop codon.
mutable provenance: The plasmid pUC-EUY which was sent to us by Ethan Johnson on iGEM Minnesota team timebomb.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
sequence annotation: cds
sequence annotation: rbs
sequence: https://synbiohub.org/public/igem/BBa_K152003_sequence/1

cds
type: SequenceAnnotation
persistent identity: annotation1999564
display id: annotation1999564
version: 1
top level: BBa_K152003
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K152003/annotation1999564/range1999564/1

https://synbiohub.org/public/igem/BBa_K152003/annotation1999564/range1999564/1
type: Range
persistent identity: range1999564
display id: range1999564
version: 1
direction: direction/forward
top level: BBa_K152003
start: 18
end: 1166
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation1999563
display id: annotation1999563
version: 1
top level: BBa_K152003
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K152003/annotation1999563/range1999563/1

https://synbiohub.org/public/igem/BBa_K152003/annotation1999563/range1999563/1
type: Range
persistent identity: range1999563
display id: range1999563
version: 1
direction: direction/forward
top level: BBa_K152003
start: 4
end: 10
orientation: inline

BBa_K152001
type: ComponentDefinition
persistent identity: BBa K152001
display id: BBa_K152001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: rbs + crtE
creator: David Johnston Monje
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:10:49Z
discontinued: false
dominant: false
experience: experience/None
group u list: _233_
m user id: 0
owner id: 2886
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: crtE from Erwinia uredovora with a rbs preceeding it. This gene codes for geranylgeranyl diphosphate synthase which is the first committed step in the beta carotene synthesis pathway.
mutable notes: We wanted to use this in synthetic operon construction, and as the first gene in the transcript, so we included a NdeI restriction site at the start codon.
mutable provenance: The plasmid pAC-3.10.10.A which was sent to us by Ethan Johnson on iGEM Minnesota team timebomb.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
sequence annotation: cds
sequence annotation: rbs
sequence: https://synbiohub.org/public/igem/BBa_K152001_sequence/1

cds
type: SequenceAnnotation
persistent identity: annotation1999568
display id: annotation1999568
version: 1
top level: BBa_K152001
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K152001/annotation1999568/range1999568/1

https://synbiohub.org/public/igem/BBa_K152001/annotation1999568/range1999568/1
type: Range
persistent identity: range1999568
display id: range1999568
version: 1
direction: direction/forward
top level: BBa_K152001
start: 18
end: 926
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation1999567
display id: annotation1999567
version: 1
top level: BBa_K152001
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K152001/annotation1999567/range1999567/1

https://synbiohub.org/public/igem/BBa_K152001/annotation1999567/range1999567/1
type: Range
persistent identity: range1999567
display id: range1999567
version: 1
direction: direction/forward
top level: BBa_K152001
start: 3
end: 10
orientation: inline

BBa_K849003
type: ComponentDefinition
persistent identity: BBa K849003
display id: BBa_K849003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: bifunctional Cyclase from Gibberella fujikuroi
creator: Martin Schneider, Sebastian Roy
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:34Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1109_
m user id: 0
owner id: 12313
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: CPS/KS
mutable notes: no
mutable provenance: Genomic sequence of Gibberella fujikuroi.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: cds
sequence: https://synbiohub.org/public/igem/BBa_K849003_sequence/1

cds
type: SequenceAnnotation
persistent identity: annotation2202327
display id: annotation2202327
version: 1
top level: BBa_K849003
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K849003/annotation2202327/range2202327/1

https://synbiohub.org/public/igem/BBa_K849003/annotation2202327/range2202327/1
type: Range
persistent identity: range2202327
display id: range2202327
version: 1
direction: direction/forward
top level: BBa_K849003
start: 1
end: 2859
orientation: inline

BBa_K118003
type: ComponentDefinition
persistent identity: BBa K118003
display id: BBa_K118003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: crtI coding sequence encoding phytoene dehydrogenase
creator: Andrew Hall
created: 2008-07-23T11:00:00Z
modified: 2015-05-08T01:09:36Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _192_
m user id: 0
owner id: 3282
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: This is the coding sequence of crtI from Pantoea ananatis (formerly Erwinia uredovora) (Accession number D90087). It encodes phytoene dehydrogenase, part of the carotenoid biosynthesis pathway, which converts Phytoene to Lycopene.
mutable notes: The native crtI sequence contains two internal PstI sites. Primers crtImutf1 and crtImutf2 were ordered so that the MABEL protocol (invented for this purpose) could be used to remove these sites. The mutagenic primers were as follows: * crtImutf1: gtt tac agt aag gtt gcc * crtImutf2: agt aac tct ctg ttt gtg c In both cases, the PstI site CTGCAG was converted to CTGCAA, a silent mutation.
mutable provenance: Pantoea ananatis genomic DNA
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: PstI site removed
sequence annotation: crtI coding sequence
sequence: https://synbiohub.org/public/igem/BBa_K118003_sequence/1

PstI site removed
type: SequenceAnnotation
persistent identity: annotation1968245
display id: annotation1968245
version: 1
top level: BBa_K118003
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_K118003/annotation1968245/range1968245/1

https://synbiohub.org/public/igem/BBa_K118003/annotation1968245/range1968245/1
type: Range
persistent identity: range1968245
display id: range1968245
version: 1
direction: direction/forward
top level: BBa_K118003
start: 889
end: 894
orientation: inline

crtI coding sequence
type: SequenceAnnotation
persistent identity: annotation1995702
display id: annotation1995702
version: 1
top level: BBa_K118003
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K118003/annotation1995702/range1995702/1

https://synbiohub.org/public/igem/BBa_K118003/annotation1995702/range1995702/1
type: Range
persistent identity: range1995702
display id: range1995702
version: 1
direction: direction/forward
top level: BBa_K118003
start: 1
end: 1479
orientation: inline

PstI site removed
type: SequenceAnnotation
persistent identity: annotation1968244
display id: annotation1968244
version: 1
top level: BBa_K118003
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_K118003/annotation1968244/range1968244/1

https://synbiohub.org/public/igem/BBa_K118003/annotation1968244/range1968244/1
type: Range
persistent identity: range1968244
display id: range1968244
version: 1
direction: direction/forward
top level: BBa_K118003
start: 478
end: 483
orientation: inline

BBa_K118006
type: ComponentDefinition
persistent identity: BBa K118006
display id: BBa_K118006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: rbs+crtB (phytoene synthase)
creator: Andrew Hall
created: 2008-08-07T11:00:00Z
modified: 2015-05-08T01:09:36Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _192_
m user id: 0
owner id: 3282
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: This is the coding sequence of crtB from Pantoea ananatis (formerly Erwinia uredovora) (Accession number D90087) preceded by an E. coli synthetic ribosome binding site (rbs). It encodes phytoene synthase, part of the carotenoid biosynthesis pathway, which converts geranylgeranyl diphosphate to phytoene.
mutable notes: No special considerations.
mutable provenance: rbs: Escherichia coli JM109 genomic DNA. crtB: Pantoea ananatis genomic DNA
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_K118002
component: BBa_J15001
sequence annotation: BBa_K118002
sequence annotation: BBa_J15001
sequence: https://synbiohub.org/public/igem/BBa_K118006_sequence/1

BBa_K118002
type: Component
persistent identity: component1970364
display id: component1970364
version: 1
top level: BBa_K118006
access: public
definition: BBa_K118002

BBa_J15001
type: Component
persistent identity: component1970363
display id: component1970363
version: 1
top level: BBa_K118006
access: public
definition: https://synbiohub.org/public/igem/BBa_J15001/1

BBa_K118002
type: SequenceAnnotation
persistent identity: annotation1970364
display id: annotation1970364
version: 1
top level: BBa_K118006
location: https://synbiohub.org/public/igem/BBa_K118006/annotation1970364/range1970364/1
component: BBa_K118002

https://synbiohub.org/public/igem/BBa_K118006/annotation1970364/range1970364/1
type: Range
persistent identity: range1970364
display id: range1970364
version: 1
direction: direction/reverse
top level: BBa_K118006
start: 17
end: 949
orientation: inline

BBa_J15001
type: SequenceAnnotation
persistent identity: annotation1970363
display id: annotation1970363
version: 1
top level: BBa_K118006
location: https://synbiohub.org/public/igem/BBa_K118006/annotation1970363/range1970363/1
component: BBa_J15001

https://synbiohub.org/public/igem/BBa_K118006/annotation1970363/range1970363/1
type: Range
persistent identity: range1970363
display id: range1970363
version: 1
direction: direction/reverse
top level: BBa_K118006
start: 1
end: 10
orientation: inline