alcohol acetyltransferase I; converts isoamyl alcohol to isoamyl acetate (banana odor)
This part is the ATF sequence (BBa_45J006) with a single point mutation at base pair 1176 (T -> C).
It has a single point mutation at base pair 1176 (T -> C).
This is an intermediate with BSMT hooked up to a terminator. It can be hooked up to a variety of RBS and promoters. This is an early step in creating a "wintergreen generating machine".
We made (cut) a B0015 backbone for this part.
It comes from the previous biobrick parts J45004 and B0015.
SAM:benzoic acid carboxyl methyltransferase; converts benzoic acid to methyl benzoate (floral odor)
BAMT catalyzes the transfer of a methyl group from SAM (S-adenosyl methyltransferase) to benzoic acid, creating methyl benzoate. Methyl benzoate produces a "pleasant" smell.
Genbank accession number(s): AF198492 This part is from A. majus.
This part is an intermediate to the protein generator BBa_45300.
SAM:salicylic acid carboxyl methyltransferase; converts salicylic acid to methyl salicylate (winter
This part can express d-limonene synthase with a constitutive promoter J23100, can it can catalyze GPPs into d-limonenen. We use this part to produce d-limonene which is said can attract nematode and some other pests well.
We searched the cDNA sequence in NCBI, and optimized the sequence for E. coli. Meanwhile, we inserted a Xho I restriction site between RBS and d-limonene synthase coding sequence, so that we can change promoter easily.
It comes from Citrus unshiu CitMTSE1 d-limonene synthase.
branched-chain amino acid transaminase (BAT2); converts leucine to alpha-ketoisocaproate
isochorismate pyruvate-lyase and isochorismate synthase (pchBA); converts chorismate to salicylate
pchBA codes for proteins used to generate salicylic acid. It doesn't make a fusion protein, even though the coding regions overlap; the RBS for pchA is located at the end of the coding region of pchB.
pchBA is found in pseudomonas. The plasmid was kindly sent to the MIT iGEM team by Dr. Cornelia Riemmann from the Universite de Lausanne.
COMT coding sequence Encodes caffeic acid-O-methyl transferase (methylates -OH on C4 of the aromatic ring) from Medicago sativa (alfalfa), synthesised by Geneart
Used cDNA as coding gene in Alfalfa genome contains introns.
Alfalfa cDNA Genes and Enzymes Involved in Caffeic Acid Biosynthesis in the Actinomycete Saccharothrix esanaensis, Berner M, et al, Journal of Bacteriology 188(7): 2666, (2006) Gowri G, et al, Molecular cloning and expression of alfalfa S-adenosyl-L-methionine: caffeic acid 3-0-methyltransferase, a key enzyme of lignin biosynthesis, Plant Physiology 97(1):7 (1991) Downregulation of Caffeic Acid 3-O-Methyltransferase and Caffeoyl CoA 3-O-Methyltransferase in Transgenic Alfalfa Impacts on Lignin Structure and Implications for the Biosynthesis of G and S Lignin, Guo D, et al, Plant Cell. January; 13(1): 73 (2001)
SAM:benzoic acid/salicylic acid carboxyl methyltransferase I; converts salicylic acid to methyl sali
Codes for BSMT enzyme to convert Benzoic acid/salicylic acid to methyl benzoate and methyl salicylate. This part is phBSMT1 from Petunia x hybrida.
Species: Petunia x hybrida from Genbank; (also available: A. thaliana and N. suaveolens)