//function/biosynthesis/plastic
type: Collection
persistent identity: function biosynthesis plastic
display id: function_biosynthesis_plastic
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
owned by: james
owned by: myers
member: BBa_K125504
member: BBa_K125801
member: BBa_K338024
member: BBa_K759004
member: BBa_K156016
member: BBa_K759009
member: BBa_K156033
member: Bioplastic
member: BBa_K759011
member: BBa_K338023
member: BBa_K156021
member: BBa_K156031
member: BBa_K759007
member: BBa_K125501
member: BBa_K338022
member: BBa_K125802
member: BBa_K156022
member: BBa_K759006
member: BBa_K156018
member: BBa_K125502
member: BBa_K759010
member: BBa_K125803
member: BBa_K338004
member: BBa_K759005
member: BBa_K156015
member: BBa_K759008
member: BBa_K125804
member: BBa_K125503
member: BBa_K338003

BBa_K338022
type: ComponentDefinition
persistent identity: BBa K338022
display id: BBa_K338022
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Plastic Intermediate: phaA + RBS
creator: Lucas Hartsough
created: 2010-10-26T11:00:00Z
modified: 2015-05-08T01:12:07Z
discontinued: false
dominant: false
group u list: _454_
m user id: 0
owner id: 6013
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: A construction intermediate used to produce <partinfo>BBa_K338003</partinfo>. Contains the first of three genes required to synthesize polyhydroxybutyrate (PHB).
mutable notes: Designed to be ligated upstream of <partinfo>BBa_K338023</partinfo> and <partinfo>BBa_K338024</partinfo>.
mutable provenance: The Registry of Standard Biological Parts
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K156012
component: BBa_B0034
sequence annotation: BBa_K156012
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K338022_sequence/1

BBa_K156012
type: Component
persistent identity: component2104851
display id: component2104851
version: 1
top level: BBa_K338022
access: public
definition: Bioplastic

BBa_B0034
type: Component
persistent identity: component2104853
display id: component2104853
version: 1
top level: BBa_K338022
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K156012
type: SequenceAnnotation
persistent identity: annotation2104851
display id: annotation2104851
version: 1
top level: BBa_K338022
location: https://synbiohub.org/public/igem/BBa_K338022/annotation2104851/range2104851/1
component: BBa_K156012

https://synbiohub.org/public/igem/BBa_K338022/annotation2104851/range2104851/1
type: Range
persistent identity: range2104851
display id: range2104851
version: 1
direction: direction/reverse
top level: BBa_K338022
start: 1
end: 1182
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2104853
display id: annotation2104853
version: 1
top level: BBa_K338022
location: https://synbiohub.org/public/igem/BBa_K338022/annotation2104853/range2104853/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K338022/annotation2104853/range2104853/1
type: Range
persistent identity: range2104853
display id: range2104853
version: 1
direction: direction/reverse
top level: BBa_K338022
start: 1191
end: 1202
orientation: inline

BBa_K338004
type: ComponentDefinition
persistent identity: BBa K338004
display id: BBa_K338004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PHA Synthase Composite, Part 2/2
creator: Lucas Hartsough
created: 2010-10-25T11:00:00Z
modified: 2015-05-08T01:12:07Z
discontinued: false
dominant: false
experience: experience/None
group u list: _454_
m user id: 0
owner id: 6013
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This is half of a planned part which would contain all three PHA synthase genes required to produce [http://en.wikipedia.org/wiki/Polyhydroxybutyrate polyhydroxybutyrate] (PHB) in cells: ''phaA'', ''phaB1'', ''phaC1''. This half contains ''phaB1'' and ''phaC1'': <partinfo>BBa_K156013</partinfo> & <partinfo>BBa_K156014</partinfo>. It was designed to be ligated downstream of part BBa_K338003.
mutable notes: When ligated downstream of BBa_K338003, the completed construct was designed to express all three PHA synthase genes required to make PHB oligomers from soybean oil. The three genes would be transcribed polycistronically on a single mRNA transcript under the IPTG-inducible control of the <partinfo>BBa_K215000</partinfo> promoter. Naturally, each gene is preceded by a standard RBS (<partinfo>B0034</partinfo>) and the transcript finishes with a strong terminator (<partinfo>B0015</partinfo>), for a total size of about 3500bp. Note that these three genes should only cause the production of PHB ''oligomers'' in cells, not hardened plastic. A crosslinking agent is required to link the oligomers and form the final plastic product. Over-expression of the ''phaC1'' gene could cause some crosslinking, but this has not been experimentally verified.
mutable provenance: Registry of Standard Biological Parts
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K156014
component: BBa_B0015
component: BBa_K156013
component: BBa_B0034
sequence annotation: BBa_K156013
sequence annotation: BBa_B0015
sequence annotation: BBa_K156014
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K338004_sequence/1

BBa_K156014
type: Component
persistent identity: component2101499
display id: component2101499
version: 1
top level: BBa_K338004
access: public
definition: Bioplastic

BBa_B0015
type: Component
persistent identity: component2101506
display id: component2101506
version: 1
top level: BBa_K338004
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_K156013
type: Component
persistent identity: component2101496
display id: component2101496
version: 1
top level: BBa_K338004
access: public
definition: Bioplastic

BBa_B0034
type: Component
persistent identity: component2101498
display id: component2101498
version: 1
top level: BBa_K338004
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K156013
type: SequenceAnnotation
persistent identity: annotation2101496
display id: annotation2101496
version: 1
top level: BBa_K338004
location: https://synbiohub.org/public/igem/BBa_K338004/annotation2101496/range2101496/1
component: BBa_K156013

https://synbiohub.org/public/igem/BBa_K338004/annotation2101496/range2101496/1
type: Range
persistent identity: range2101496
display id: range2101496
version: 1
direction: direction/reverse
top level: BBa_K338004
start: 1
end: 741
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2101506
display id: annotation2101506
version: 1
top level: BBa_K338004
location: https://synbiohub.org/public/igem/BBa_K338004/annotation2101506/range2101506/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K338004/annotation2101506/range2101506/1
type: Range
persistent identity: range2101506
display id: range2101506
version: 1
direction: direction/reverse
top level: BBa_K338004
start: 2125
end: 2253
orientation: inline

BBa_K156014
type: SequenceAnnotation
persistent identity: annotation2101499
display id: annotation2101499
version: 1
top level: BBa_K338004
location: https://synbiohub.org/public/igem/BBa_K338004/annotation2101499/range2101499/1
component: BBa_K156014

https://synbiohub.org/public/igem/BBa_K338004/annotation2101499/range2101499/1
type: Range
persistent identity: range2101499
display id: range2101499
version: 1
direction: direction/reverse
top level: BBa_K338004
start: 768
end: 2116
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2101498
display id: annotation2101498
version: 1
top level: BBa_K338004
location: https://synbiohub.org/public/igem/BBa_K338004/annotation2101498/range2101498/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K338004/annotation2101498/range2101498/1
type: Range
persistent identity: range2101498
display id: range2101498
version: 1
direction: direction/reverse
top level: BBa_K338004
start: 750
end: 761
orientation: inline

BBa_K125802
type: ComponentDefinition
persistent identity: BBa K125802
display id: BBa_K125802
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS-phaB
creator: Norman Wang
created: 2008-09-02T11:00:00Z
modified: 2015-05-08T01:09:45Z
discontinued: false
dominant: false
experience: experience/None
group u list: _179_
m user id: 0
owner id: 2680
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: RBS 1.0 + phaB gene
mutable notes: composite part
mutable provenance: composite part
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_K125502
component: BBa_B0034
sequence annotation: BBa_K125502
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K125802_sequence/1

BBa_K125502
type: Component
persistent identity: component1974232
display id: component1974232
version: 1
top level: BBa_K125802
access: public
definition: BBa_K125502

BBa_B0034
type: Component
persistent identity: component1974228
display id: component1974228
version: 1
top level: BBa_K125802
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K125502
type: SequenceAnnotation
persistent identity: annotation1974232
display id: annotation1974232
version: 1
top level: BBa_K125802
location: https://synbiohub.org/public/igem/BBa_K125802/annotation1974232/range1974232/1
component: BBa_K125502

https://synbiohub.org/public/igem/BBa_K125802/annotation1974232/range1974232/1
type: Range
persistent identity: range1974232
display id: range1974232
version: 1
direction: direction/reverse
top level: BBa_K125802
start: 19
end: 744
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1974228
display id: annotation1974228
version: 1
top level: BBa_K125802
location: https://synbiohub.org/public/igem/BBa_K125802/annotation1974228/range1974228/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K125802/annotation1974228/range1974228/1
type: Range
persistent identity: range1974228
display id: range1974228
version: 1
direction: direction/reverse
top level: BBa_K125802
start: 1
end: 12
orientation: inline

BBa_K125803
type: ComponentDefinition
persistent identity: BBa K125803
display id: BBa_K125803
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS-phaC
creator: Norman Wang
created: 2008-09-02T11:00:00Z
modified: 2015-05-08T01:09:45Z
discontinued: false
dominant: false
experience: experience/None
group u list: _179_
m user id: 0
owner id: 2680
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: RBS 1.0 + phaC gene
mutable notes: composite part
mutable provenance: composite part
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_B0034
component: BBa_K125503
sequence annotation: BBa_K125503
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K125803_sequence/1

BBa_B0034
type: Component
persistent identity: component1974234
display id: component1974234
version: 1
top level: BBa_K125803
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K125503
type: Component
persistent identity: component1974238
display id: component1974238
version: 1
top level: BBa_K125803
access: public
definition: BBa_K125503

BBa_K125503
type: SequenceAnnotation
persistent identity: annotation1974238
display id: annotation1974238
version: 1
top level: BBa_K125803
location: https://synbiohub.org/public/igem/BBa_K125803/annotation1974238/range1974238/1
component: BBa_K125503

https://synbiohub.org/public/igem/BBa_K125803/annotation1974238/range1974238/1
type: Range
persistent identity: range1974238
display id: range1974238
version: 1
direction: direction/reverse
top level: BBa_K125803
start: 19
end: 1158
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1974234
display id: annotation1974234
version: 1
top level: BBa_K125803
location: https://synbiohub.org/public/igem/BBa_K125803/annotation1974234/range1974234/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K125803/annotation1974234/range1974234/1
type: Range
persistent identity: range1974234
display id: range1974234
version: 1
direction: direction/reverse
top level: BBa_K125803
start: 1
end: 12
orientation: inline

BBa_K759011
type: ComponentDefinition
persistent identity: BBa K759011
display id: BBa_K759011
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: P3HB producing construct
creator: Ami Kawahata
created: 2012-09-19T11:00:00Z
modified: 2015-05-08T01:13:13Z
discontinued: false
dominant: false
group u list: _1010_
m user id: 0
owner id: 11723
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This is an construct for producing P3HB. A constitutive promoter(BBa_R0040) locates on the upstream of the whole construct and by adding 2 percent gulcose into the media, the bacteria would produce bioplastics.
mutable notes: Nothing to remark.
mutable provenance: phaA and phaB was cloned from pGEM phbCAB which carries the gene of Ralstonia Eutropha.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K759004
component: BBa_B0034
component: BBa_K759005
component: BBa_K342001
component: BBa_B0015
component: BBa_R0040
sequence annotation: BBa_K759005
sequence annotation: BBa_K342001
sequence annotation: BBa_K759004
sequence annotation: BBa_B0034
sequence annotation: BBa_R0040
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_K759011_sequence/1

BBa_K759004
type: Component
persistent identity: component2188478
display id: component2188478
version: 1
top level: BBa_K759011
access: public
definition: BBa_K759004

BBa_B0034
type: Component
persistent identity: component2188477
display id: component2188477
version: 1
top level: BBa_K759011
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K759005
type: Component
persistent identity: component2188481
display id: component2188481
version: 1
top level: BBa_K759011
access: public
definition: BBa_K759005

BBa_B0034
type: Component
persistent identity: component2188474
display id: component2188474
version: 1
top level: BBa_K759011
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K342001
type: Component
persistent identity: component2188475
display id: component2188475
version: 1
top level: BBa_K759011
access: public
definition: https://synbiohub.org/public/igem/BBa_K342001/1

BBa_B0015
type: Component
persistent identity: component2188488
display id: component2188488
version: 1
top level: BBa_K759011
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_R0040
type: Component
persistent identity: component2188468
display id: component2188468
version: 1
top level: BBa_K759011
access: public
definition: https://synbiohub.org/public/igem/BBa_R0040/1

BBa_B0034
type: Component
persistent identity: component2188480
display id: component2188480
version: 1
top level: BBa_K759011
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K759005
type: SequenceAnnotation
persistent identity: annotation2188481
display id: annotation2188481
version: 1
top level: BBa_K759011
location: https://synbiohub.org/public/igem/BBa_K759011/annotation2188481/range2188481/1
component: BBa_K759005

https://synbiohub.org/public/igem/BBa_K759011/annotation2188481/range2188481/1
type: Range
persistent identity: range2188481
display id: range2188481
version: 1
direction: direction/reverse
top level: BBa_K759011
start: 3106
end: 3843
orientation: inline

BBa_K342001
type: SequenceAnnotation
persistent identity: annotation2188475
display id: annotation2188475
version: 1
top level: BBa_K759011
location: https://synbiohub.org/public/igem/BBa_K759011/annotation2188475/range2188475/1
component: BBa_K342001

https://synbiohub.org/public/igem/BBa_K759011/annotation2188475/range2188475/1
type: Range
persistent identity: range2188475
display id: range2188475
version: 1
direction: direction/reverse
top level: BBa_K759011
start: 83
end: 1871
orientation: inline

BBa_K759004
type: SequenceAnnotation
persistent identity: annotation2188478
display id: annotation2188478
version: 1
top level: BBa_K759011
location: https://synbiohub.org/public/igem/BBa_K759011/annotation2188478/range2188478/1
component: BBa_K759004

https://synbiohub.org/public/igem/BBa_K759011/annotation2188478/range2188478/1
type: Range
persistent identity: range2188478
display id: range2188478
version: 1
direction: direction/reverse
top level: BBa_K759011
start: 1898
end: 3079
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2188480
display id: annotation2188480
version: 1
top level: BBa_K759011
location: https://synbiohub.org/public/igem/BBa_K759011/annotation2188480/range2188480/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K759011/annotation2188480/range2188480/1
type: Range
persistent identity: range2188480
display id: range2188480
version: 1
direction: direction/reverse
top level: BBa_K759011
start: 3088
end: 3099
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation2188468
display id: annotation2188468
version: 1
top level: BBa_K759011
location: https://synbiohub.org/public/igem/BBa_K759011/annotation2188468/range2188468/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_K759011/annotation2188468/range2188468/1
type: Range
persistent identity: range2188468
display id: range2188468
version: 1
direction: direction/forward
top level: BBa_K759011
start: 1
end: 54
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2188474
display id: annotation2188474
version: 1
top level: BBa_K759011
location: https://synbiohub.org/public/igem/BBa_K759011/annotation2188474/range2188474/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K759011/annotation2188474/range2188474/1
type: Range
persistent identity: range2188474
display id: range2188474
version: 1
direction: direction/reverse
top level: BBa_K759011
start: 63
end: 74
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2188488
display id: annotation2188488
version: 1
top level: BBa_K759011
location: https://synbiohub.org/public/igem/BBa_K759011/annotation2188488/range2188488/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K759011/annotation2188488/range2188488/1
type: Range
persistent identity: range2188488
display id: range2188488
version: 1
direction: direction/reverse
top level: BBa_K759011
start: 3852
end: 3980
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2188477
display id: annotation2188477
version: 1
top level: BBa_K759011
location: https://synbiohub.org/public/igem/BBa_K759011/annotation2188477/range2188477/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K759011/annotation2188477/range2188477/1
type: Range
persistent identity: range2188477
display id: range2188477
version: 1
direction: direction/reverse
top level: BBa_K759011
start: 1880
end: 1891
orientation: inline

BBa_K338023
type: ComponentDefinition
persistent identity: BBa K338023
display id: BBa_K338023
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Plastic Intermediate: phaB1 + RBS
creator: Lucas Hartsough
created: 2010-10-26T11:00:00Z
modified: 2015-05-08T01:12:07Z
discontinued: false
dominant: false
group u list: _454_
m user id: 0
owner id: 6013
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: A construction intermediate used to produce <partinfo>K338004</partinfo>. Contains the second of three genes required to synthesize polyhydroxybutyrate (PHB).
mutable notes: This part was designed to be ligated between <partinfo>BBa_K3380022</partinfo> and <partinfo>BBa_K338024</partinfo>.
mutable provenance: The Registry of Standard Biological Parts
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K156013
component: BBa_B0034
sequence annotation: BBa_B0034
sequence annotation: BBa_K156013
sequence: https://synbiohub.org/public/igem/BBa_K338023_sequence/1

BBa_K156013
type: Component
persistent identity: component2104807
display id: component2104807
version: 1
top level: BBa_K338023
access: public
definition: Bioplastic

BBa_B0034
type: Component
persistent identity: component2104809
display id: component2104809
version: 1
top level: BBa_K338023
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2104809
display id: annotation2104809
version: 1
top level: BBa_K338023
location: https://synbiohub.org/public/igem/BBa_K338023/annotation2104809/range2104809/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K338023/annotation2104809/range2104809/1
type: Range
persistent identity: range2104809
display id: range2104809
version: 1
direction: direction/reverse
top level: BBa_K338023
start: 750
end: 761
orientation: inline

BBa_K156013
type: SequenceAnnotation
persistent identity: annotation2104807
display id: annotation2104807
version: 1
top level: BBa_K338023
location: https://synbiohub.org/public/igem/BBa_K338023/annotation2104807/range2104807/1
component: BBa_K156013

https://synbiohub.org/public/igem/BBa_K338023/annotation2104807/range2104807/1
type: Range
persistent identity: range2104807
display id: range2104807
version: 1
direction: direction/reverse
top level: BBa_K338023
start: 1
end: 741
orientation: inline

BBa_K759008
type: ComponentDefinition
persistent identity: BBa K759008
display id: BBa_K759008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: B0034 and phaC (BBa_K342001)
creator: Ami Kawahata
created: 2012-09-19T11:00:00Z
modified: 2015-05-08T01:13:13Z
discontinued: false
dominant: false
group u list: _1010_
m user id: 0
owner id: 11723
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: A composite of RBS(b0034) and phaC(BBa_K342001).
mutable notes: Nothing to remark.
mutable provenance: phaC is a Bio brick registered by 10_ISNA Lyon.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_K342001
component: BBa_B0034
sequence annotation: BBa_K342001
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K759008_sequence/1

BBa_K342001
type: Component
persistent identity: component2187531
display id: component2187531
version: 1
top level: BBa_K759008
access: public
definition: https://synbiohub.org/public/igem/BBa_K342001/1

BBa_B0034
type: Component
persistent identity: component2187530
display id: component2187530
version: 1
top level: BBa_K759008
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K342001
type: SequenceAnnotation
persistent identity: annotation2187531
display id: annotation2187531
version: 1
top level: BBa_K759008
location: https://synbiohub.org/public/igem/BBa_K759008/annotation2187531/range2187531/1
component: BBa_K342001

https://synbiohub.org/public/igem/BBa_K759008/annotation2187531/range2187531/1
type: Range
persistent identity: range2187531
display id: range2187531
version: 1
direction: direction/reverse
top level: BBa_K759008
start: 21
end: 1809
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2187530
display id: annotation2187530
version: 1
top level: BBa_K759008
location: https://synbiohub.org/public/igem/BBa_K759008/annotation2187530/range2187530/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K759008/annotation2187530/range2187530/1
type: Range
persistent identity: range2187530
display id: range2187530
version: 1
direction: direction/reverse
top level: BBa_K759008
start: 1
end: 12
orientation: inline

BBa_K125801
type: ComponentDefinition
persistent identity: BBa K125801
display id: BBa_K125801
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS-phaA
creator: Norman Wang
created: 2008-09-02T11:00:00Z
modified: 2015-05-08T01:09:45Z
discontinued: false
dominant: false
experience: experience/None
group u list: _179_
m user id: 0
owner id: 2680
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: RBS 1.0 + phaA gene
mutable notes: composite part
mutable provenance: composite part
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_K125501
component: BBa_B0034
sequence annotation: BBa_B0034
sequence annotation: BBa_K125501
sequence: https://synbiohub.org/public/igem/BBa_K125801_sequence/1

BBa_K125501
type: Component
persistent identity: component1974226
display id: component1974226
version: 1
top level: BBa_K125801
access: public
definition: BBa_K125501

BBa_B0034
type: Component
persistent identity: component1974222
display id: component1974222
version: 1
top level: BBa_K125801
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1974222
display id: annotation1974222
version: 1
top level: BBa_K125801
location: https://synbiohub.org/public/igem/BBa_K125801/annotation1974222/range1974222/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K125801/annotation1974222/range1974222/1
type: Range
persistent identity: range1974222
display id: range1974222
version: 1
direction: direction/reverse
top level: BBa_K125801
start: 1
end: 12
orientation: inline

BBa_K125501
type: SequenceAnnotation
persistent identity: annotation1974226
display id: annotation1974226
version: 1
top level: BBa_K125801
location: https://synbiohub.org/public/igem/BBa_K125801/annotation1974226/range1974226/1
component: BBa_K125501

https://synbiohub.org/public/igem/BBa_K125801/annotation1974226/range1974226/1
type: Range
persistent identity: range1974226
display id: range1974226
version: 1
direction: direction/reverse
top level: BBa_K125801
start: 19
end: 1251
orientation: inline

BBa_K125502
type: ComponentDefinition
persistent identity: BBa K125502
display id: BBa_K125502
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: phaB BioPlastic polyhydroxybutyrate synthesis pathway (origin PCC6803 slr1993)
creator: Norman Wang
created: 2008-08-09T11:00:00Z
modified: 2015-05-08T01:09:45Z
discontinued: false
dominant: false
group u list: _179_
m user id: 0
owner id: 2680
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: phaB
mutable notes: PHB biosynthesis pathway
mutable provenance: Synechocystis sp. PCC6803
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: inserted second stop
sequence annotation: start
sequence annotation: original stop codon
sequence: https://synbiohub.org/public/igem/BBa_K125502_sequence/1

inserted second stop
type: SequenceAnnotation
persistent identity: annotation1971710
display id: annotation1971710
version: 1
top level: BBa_K125502
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K125502/annotation1971710/range1971710/1

https://synbiohub.org/public/igem/BBa_K125502/annotation1971710/range1971710/1
type: Range
persistent identity: range1971710
display id: range1971710
version: 1
direction: direction/forward
top level: BBa_K125502
start: 724
end: 726
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation1971708
display id: annotation1971708
version: 1
top level: BBa_K125502
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K125502/annotation1971708/range1971708/1

https://synbiohub.org/public/igem/BBa_K125502/annotation1971708/range1971708/1
type: Range
persistent identity: range1971708
display id: range1971708
version: 1
direction: direction/forward
top level: BBa_K125502
start: 1
end: 3
orientation: inline

original stop codon
type: SequenceAnnotation
persistent identity: annotation1971709
display id: annotation1971709
version: 1
top level: BBa_K125502
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K125502/annotation1971709/range1971709/1

https://synbiohub.org/public/igem/BBa_K125502/annotation1971709/range1971709/1
type: Range
persistent identity: range1971709
display id: range1971709
version: 1
direction: direction/forward
top level: BBa_K125502
start: 721
end: 723
orientation: inline

BBa_K156021
type: ComponentDefinition
persistent identity: BBa K156021
display id: BBa_K156021
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter + RBS + phaA + double terminator
creator: George McArthur IV
created: 2008-10-28T12:00:00Z
modified: 2015-05-08T01:10:54Z
discontinued: false
dominant: false
experience: experience/None
group u list: _246_
m user id: 0
owner id: 1475
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This device is a synthetic gene that generates PhaA, an enzyme that catalyzes the synthesis of acetoacetyl-coenzyme A from two molecules of acetyl-coenzyme A. The enzyme can also act as a thiolase, catalyzing the reverse reaction and generating two-carbon units from the four-carbon product of fatty acid oxidation.
mutable notes: No special design considerations.
mutable provenance: This is a composite part.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_R0040
component: BBa_B0010
component: BBa_B0032
component: BBa_B0012
component: BBa_K156012
sequence annotation: BBa_B0010
sequence annotation: BBa_R0040
sequence annotation: BBa_B0032
sequence annotation: BBa_K156012
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_K156021_sequence/1

BBa_R0040
type: Component
persistent identity: component1993795
display id: component1993795
version: 1
top level: BBa_K156021
access: public
definition: https://synbiohub.org/public/igem/BBa_R0040/1

BBa_B0010
type: Component
persistent identity: component1993804
display id: component1993804
version: 1
top level: BBa_K156021
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0032
type: Component
persistent identity: component1993801
display id: component1993801
version: 1
top level: BBa_K156021
access: public
definition: https://synbiohub.org/public/igem/BBa_B0032/1

BBa_B0012
type: Component
persistent identity: component1993806
display id: component1993806
version: 1
top level: BBa_K156021
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_K156012
type: Component
persistent identity: component1993803
display id: component1993803
version: 1
top level: BBa_K156021
access: public
definition: Bioplastic

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1993804
display id: annotation1993804
version: 1
top level: BBa_K156021
location: https://synbiohub.org/public/igem/BBa_K156021/annotation1993804/range1993804/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K156021/annotation1993804/range1993804/1
type: Range
persistent identity: range1993804
display id: range1993804
version: 1
direction: direction/forward
top level: BBa_K156021
start: 1272
end: 1351
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1993795
display id: annotation1993795
version: 1
top level: BBa_K156021
location: https://synbiohub.org/public/igem/BBa_K156021/annotation1993795/range1993795/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_K156021/annotation1993795/range1993795/1
type: Range
persistent identity: range1993795
display id: range1993795
version: 1
direction: direction/forward
top level: BBa_K156021
start: 1
end: 54
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation1993801
display id: annotation1993801
version: 1
top level: BBa_K156021
location: https://synbiohub.org/public/igem/BBa_K156021/annotation1993801/range1993801/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_K156021/annotation1993801/range1993801/1
type: Range
persistent identity: range1993801
display id: range1993801
version: 1
direction: direction/forward
top level: BBa_K156021
start: 63
end: 75
orientation: inline

BBa_K156012
type: SequenceAnnotation
persistent identity: annotation1993803
display id: annotation1993803
version: 1
top level: BBa_K156021
location: https://synbiohub.org/public/igem/BBa_K156021/annotation1993803/range1993803/1
component: BBa_K156012

https://synbiohub.org/public/igem/BBa_K156021/annotation1993803/range1993803/1
type: Range
persistent identity: range1993803
display id: range1993803
version: 1
direction: direction/reverse
top level: BBa_K156021
start: 82
end: 1263
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1993806
display id: annotation1993806
version: 1
top level: BBa_K156021
location: https://synbiohub.org/public/igem/BBa_K156021/annotation1993806/range1993806/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K156021/annotation1993806/range1993806/1
type: Range
persistent identity: range1993806
display id: range1993806
version: 1
direction: direction/forward
top level: BBa_K156021
start: 1360
end: 1400
orientation: inline

Bioplastic
type: ComponentDefinition
persistent identity: BBa K156013
display id: BBa_K156013
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: phaB1 (acetyacetyl-CoA reductase)
creator: George McArthur IV
created: 2008-10-28T12:00:00Z
modified: 2015-05-08T01:10:54Z
discontinued: false
dominant: false
experience: experience/None
group u list: _246_
m user id: 0
owner id: 1475
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part codes for an enzyme that catalyzes the formation of 3-hydroxybutyryl-CoA from acetoacetyl-CoA in polyhydroxyalkanoate synthesis.
mutable notes: This part was codon-optimized for expression in E. coli. The following restriction enzyme sites were also removed: EcoRI, NotI, XbaI, SpeI, PstI, NheI, AvrII, ApoI, MfeI, NsiI, SbfI.
mutable provenance: This coding region is native to Ralstonia eutropha H16. The original sequence was obtained from GenBank (VERSION NC_008313.1, GI:113866031).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K156013_sequence/1

BBa_K156031
type: ComponentDefinition
persistent identity: BBa K156031
display id: BBa_K156031
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS + phaA + double terminator
creator: Daniel R Tarjan
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:10:54Z
discontinued: false
dominant: false
experience: experience/None
group u list: _246_
m user id: 0
owner id: 2922
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This part is a composite part made of a standard ribosomal binding site, a part that codes for an acetyl-CoA acetyltransferase (PhaA), and a terminator (B0015).
mutable notes: None.
mutable provenance: This is a composite part.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0010
component: BBa_K156012
component: BBa_B0032
component: BBa_B0012
sequence annotation: BBa_B0032
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_K156012
sequence: https://synbiohub.org/public/igem/BBa_K156031_sequence/1

BBa_B0010
type: Component
persistent identity: component1997062
display id: component1997062
version: 1
top level: BBa_K156031
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_K156012
type: Component
persistent identity: component1997061
display id: component1997061
version: 1
top level: BBa_K156031
access: public
definition: Bioplastic

BBa_B0032
type: Component
persistent identity: component1997059
display id: component1997059
version: 1
top level: BBa_K156031
access: public
definition: https://synbiohub.org/public/igem/BBa_B0032/1

BBa_B0012
type: Component
persistent identity: component1997064
display id: component1997064
version: 1
top level: BBa_K156031
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation1997059
display id: annotation1997059
version: 1
top level: BBa_K156031
location: https://synbiohub.org/public/igem/BBa_K156031/annotation1997059/range1997059/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_K156031/annotation1997059/range1997059/1
type: Range
persistent identity: range1997059
display id: range1997059
version: 1
direction: direction/forward
top level: BBa_K156031
start: 1
end: 13
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1997062
display id: annotation1997062
version: 1
top level: BBa_K156031
location: https://synbiohub.org/public/igem/BBa_K156031/annotation1997062/range1997062/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K156031/annotation1997062/range1997062/1
type: Range
persistent identity: range1997062
display id: range1997062
version: 1
direction: direction/forward
top level: BBa_K156031
start: 1210
end: 1289
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1997064
display id: annotation1997064
version: 1
top level: BBa_K156031
location: https://synbiohub.org/public/igem/BBa_K156031/annotation1997064/range1997064/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K156031/annotation1997064/range1997064/1
type: Range
persistent identity: range1997064
display id: range1997064
version: 1
direction: direction/forward
top level: BBa_K156031
start: 1298
end: 1338
orientation: inline

BBa_K156012
type: SequenceAnnotation
persistent identity: annotation1997061
display id: annotation1997061
version: 1
top level: BBa_K156031
location: https://synbiohub.org/public/igem/BBa_K156031/annotation1997061/range1997061/1
component: BBa_K156012

https://synbiohub.org/public/igem/BBa_K156031/annotation1997061/range1997061/1
type: Range
persistent identity: range1997061
display id: range1997061
version: 1
direction: direction/reverse
top level: BBa_K156031
start: 20
end: 1201
orientation: inline

Bioplastic
type: ComponentDefinition
persistent identity: BBa K156014
display id: BBa_K156014
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: phaC1 (Poly(3-hydroxybutyrate) polymerase)
creator: George McArthur IV
created: 2008-10-28T12:00:00Z
modified: 2015-05-08T01:10:54Z
discontinued: false
dominant: false
experience: experience/None
group u list: _246_
m user id: 0
owner id: 1475
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part codes for a polyhydroxybutyrate (PHB) synthase, which catalyzes the polymerization of hydroxybutyryl-CoA.
mutable notes: This part was codon-optimized for expression in E. coli. The following restriction enzyme sites were also removed: EcoRI, NotI, XbaI, SpeI, PstI, NheI, AvrII, ApoI, MfeI, NsiI, SbfI.
mutable provenance: This coding region is native to Ralstonia eutropha H16. The original sequence was obtained from GenBank (VERSION NC_008313.1, GI:113866031).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K156014_sequence/1

BBa_K759006
type: ComponentDefinition
persistent identity: BBa K759006
display id: BBa_K759006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: B0034 and phaA (beta-ketothiolase orgin from Ralstonia eutropha)
creator: Ami Kawahata
created: 2012-09-18T11:00:00Z
modified: 2015-05-08T01:13:13Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1010_
m user id: 0
owner id: 11723
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This is a construct of RBS and phaA.
mutable notes: Nothing to remark.
mutable provenance: PhaA was cloned from pGEM phbCAB which carries the synthetic operon genes (phaA,phaB,phaC) of Ralstonia eutropha.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_K759004
component: BBa_B0034
sequence annotation: BBa_K759004
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K759006_sequence/1

BBa_K759004
type: Component
persistent identity: component2187329
display id: component2187329
version: 1
top level: BBa_K759006
access: public
definition: BBa_K759004

BBa_B0034
type: Component
persistent identity: component2187328
display id: component2187328
version: 1
top level: BBa_K759006
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K759004
type: SequenceAnnotation
persistent identity: annotation2187329
display id: annotation2187329
version: 1
top level: BBa_K759006
location: https://synbiohub.org/public/igem/BBa_K759006/annotation2187329/range2187329/1
component: BBa_K759004

https://synbiohub.org/public/igem/BBa_K759006/annotation2187329/range2187329/1
type: Range
persistent identity: range2187329
display id: range2187329
version: 1
direction: direction/reverse
top level: BBa_K759006
start: 19
end: 1200
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2187328
display id: annotation2187328
version: 1
top level: BBa_K759006
location: https://synbiohub.org/public/igem/BBa_K759006/annotation2187328/range2187328/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K759006/annotation2187328/range2187328/1
type: Range
persistent identity: range2187328
display id: range2187328
version: 1
direction: direction/reverse
top level: BBa_K759006
start: 1
end: 12
orientation: inline

BBa_K125504
type: ComponentDefinition
persistent identity: BBa K125504
display id: BBa_K125504
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: phaE BioPlastic polyhydroxybutyrate synthesis pathway (origin PCC6803 slr1829)
creator: Norman Wang
created: 2008-08-09T11:00:00Z
modified: 2015-05-08T01:09:45Z
discontinued: false
dominant: false
group u list: _179_
m user id: 0
owner id: 2680
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: phaE
mutable notes: PHB biosynthesis pathway
mutable provenance: Synechocystis sp. PCC6803
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: start
sequence annotation: original stop codon
sequence annotation: E. coli codon optimized, Ile ATT -> ATC, avoided rare Ile ATA
sequence annotation: inserted second stop
sequence: https://synbiohub.org/public/igem/BBa_K125504_sequence/1

start
type: SequenceAnnotation
persistent identity: annotation1971714
display id: annotation1971714
version: 1
top level: BBa_K125504
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K125504/annotation1971714/range1971714/1

https://synbiohub.org/public/igem/BBa_K125504/annotation1971714/range1971714/1
type: Range
persistent identity: range1971714
display id: range1971714
version: 1
direction: direction/forward
top level: BBa_K125504
start: 1
end: 3
orientation: inline

original stop codon
type: SequenceAnnotation
persistent identity: annotation1971715
display id: annotation1971715
version: 1
top level: BBa_K125504
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K125504/annotation1971715/range1971715/1

https://synbiohub.org/public/igem/BBa_K125504/annotation1971715/range1971715/1
type: Range
persistent identity: range1971715
display id: range1971715
version: 1
direction: direction/forward
top level: BBa_K125504
start: 991
end: 993
orientation: inline

E. coli codon optimized, Ile ATT -> ATC, avoided rare Ile ATA
type: SequenceAnnotation
persistent identity: annotation1971717
display id: annotation1971717
version: 1
top level: BBa_K125504
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_K125504/annotation1971717/range1971717/1

https://synbiohub.org/public/igem/BBa_K125504/annotation1971717/range1971717/1
type: Range
persistent identity: range1971717
display id: range1971717
version: 1
direction: direction/forward
top level: BBa_K125504
start: 855
end: 855
orientation: inline

inserted second stop
type: SequenceAnnotation
persistent identity: annotation1971716
display id: annotation1971716
version: 1
top level: BBa_K125504
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K125504/annotation1971716/range1971716/1

https://synbiohub.org/public/igem/BBa_K125504/annotation1971716/range1971716/1
type: Range
persistent identity: range1971716
display id: range1971716
version: 1
direction: direction/forward
top level: BBa_K125504
start: 994
end: 996
orientation: inline

BBa_K156033
type: ComponentDefinition
persistent identity: BBa K156033
display id: BBa_K156033
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS + phaB1 + double terminator
creator: Daniel R Tarjan
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:10:54Z
discontinued: false
dominant: true
experience: experience/None
group u list: _246_
m user id: 0
owner id: 2922
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This composite part is made of a standard ribosomal binding site and a part that codes for an enzyme that catalyzes the formation of 3-hydroxybutyryl-CoA from acetoacetyl-CoA in polyhydroxyalkanoate synthesis, and a terminator.
mutable notes: This is a composite part.
mutable provenance: None
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0010
component: BBa_K156013
component: BBa_B0012
component: BBa_B0032
sequence annotation: BBa_B0032
sequence annotation: BBa_K156013
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_K156033_sequence/1

BBa_B0010
type: Component
persistent identity: component1997088
display id: component1997088
version: 1
top level: BBa_K156033
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_K156013
type: Component
persistent identity: component1997087
display id: component1997087
version: 1
top level: BBa_K156033
access: public
definition: Bioplastic

BBa_B0012
type: Component
persistent identity: component1997090
display id: component1997090
version: 1
top level: BBa_K156033
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0032
type: Component
persistent identity: component1997085
display id: component1997085
version: 1
top level: BBa_K156033
access: public
definition: https://synbiohub.org/public/igem/BBa_B0032/1

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation1997085
display id: annotation1997085
version: 1
top level: BBa_K156033
location: https://synbiohub.org/public/igem/BBa_K156033/annotation1997085/range1997085/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_K156033/annotation1997085/range1997085/1
type: Range
persistent identity: range1997085
display id: range1997085
version: 1
direction: direction/forward
top level: BBa_K156033
start: 1
end: 13
orientation: inline

BBa_K156013
type: SequenceAnnotation
persistent identity: annotation1997087
display id: annotation1997087
version: 1
top level: BBa_K156033
location: https://synbiohub.org/public/igem/BBa_K156033/annotation1997087/range1997087/1
component: BBa_K156013

https://synbiohub.org/public/igem/BBa_K156033/annotation1997087/range1997087/1
type: Range
persistent identity: range1997087
display id: range1997087
version: 1
direction: direction/reverse
top level: BBa_K156033
start: 20
end: 760
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1997088
display id: annotation1997088
version: 1
top level: BBa_K156033
location: https://synbiohub.org/public/igem/BBa_K156033/annotation1997088/range1997088/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K156033/annotation1997088/range1997088/1
type: Range
persistent identity: range1997088
display id: range1997088
version: 1
direction: direction/forward
top level: BBa_K156033
start: 769
end: 848
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1997090
display id: annotation1997090
version: 1
top level: BBa_K156033
location: https://synbiohub.org/public/igem/BBa_K156033/annotation1997090/range1997090/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K156033/annotation1997090/range1997090/1
type: Range
persistent identity: range1997090
display id: range1997090
version: 1
direction: direction/forward
top level: BBa_K156033
start: 857
end: 897
orientation: inline

BBa_K125804
type: ComponentDefinition
persistent identity: BBa K125804
display id: BBa_K125804
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS-phaE
creator: Norman Wang
created: 2008-09-02T11:00:00Z
modified: 2015-05-08T01:09:45Z
discontinued: false
dominant: false
experience: experience/None
group u list: _179_
m user id: 0
owner id: 2680
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: RBS 1.0 + phaE gene
mutable notes: composite part
mutable provenance: composite part
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_B0034
component: BBa_K125504
sequence annotation: BBa_B0034
sequence annotation: BBa_K125504
sequence: https://synbiohub.org/public/igem/BBa_K125804_sequence/1

BBa_B0034
type: Component
persistent identity: component1974240
display id: component1974240
version: 1
top level: BBa_K125804
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K125504
type: Component
persistent identity: component1974245
display id: component1974245
version: 1
top level: BBa_K125804
access: public
definition: BBa_K125504

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1974240
display id: annotation1974240
version: 1
top level: BBa_K125804
location: https://synbiohub.org/public/igem/BBa_K125804/annotation1974240/range1974240/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K125804/annotation1974240/range1974240/1
type: Range
persistent identity: range1974240
display id: range1974240
version: 1
direction: direction/reverse
top level: BBa_K125804
start: 1
end: 12
orientation: inline

BBa_K125504
type: SequenceAnnotation
persistent identity: annotation1974245
display id: annotation1974245
version: 1
top level: BBa_K125804
location: https://synbiohub.org/public/igem/BBa_K125804/annotation1974245/range1974245/1
component: BBa_K125504

https://synbiohub.org/public/igem/BBa_K125804/annotation1974245/range1974245/1
type: Range
persistent identity: range1974245
display id: range1974245
version: 1
direction: direction/reverse
top level: BBa_K125804
start: 19
end: 1014
orientation: inline

BBa_K125503
type: ComponentDefinition
persistent identity: BBa K125503
display id: BBa_K125503
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: phaC BioPlastic polyhydroxybutyrate synthesis pathway (origin PCC6803 slr1830)
creator: Norman Wang
created: 2008-08-09T11:00:00Z
modified: 2015-05-08T01:09:45Z
discontinued: false
dominant: false
group u list: _179_
m user id: 0
owner id: 2680
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: phaC
mutable notes: PHB biosynthesis pathway
mutable provenance: Synechocystis sp. PCC6803
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: inserted second stop
sequence annotation: mt tGa to tAa
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_K125503_sequence/1

inserted second stop
type: SequenceAnnotation
persistent identity: annotation1971713
display id: annotation1971713
version: 1
top level: BBa_K125503
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K125503/annotation1971713/range1971713/1

https://synbiohub.org/public/igem/BBa_K125503/annotation1971713/range1971713/1
type: Range
persistent identity: range1971713
display id: range1971713
version: 1
direction: direction/forward
top level: BBa_K125503
start: 1138
end: 1140
orientation: inline

mt tGa to tAa
type: SequenceAnnotation
persistent identity: annotation1971712
display id: annotation1971712
version: 1
top level: BBa_K125503
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K125503/annotation1971712/range1971712/1

https://synbiohub.org/public/igem/BBa_K125503/annotation1971712/range1971712/1
type: Range
persistent identity: range1971712
display id: range1971712
version: 1
direction: direction/forward
top level: BBa_K125503
start: 1135
end: 1137
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation1971711
display id: annotation1971711
version: 1
top level: BBa_K125503
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K125503/annotation1971711/range1971711/1

https://synbiohub.org/public/igem/BBa_K125503/annotation1971711/range1971711/1
type: Range
persistent identity: range1971711
display id: range1971711
version: 1
direction: direction/forward
top level: BBa_K125503
start: 1
end: 3
orientation: inline

BBa_K156022
type: ComponentDefinition
persistent identity: BBa K156022
display id: BBa_K156022
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter + RBS + phaB1 + double terminator
creator: George McArthur IV
created: 2008-10-28T12:00:00Z
modified: 2015-05-08T01:10:54Z
discontinued: false
dominant: false
experience: experience/None
group u list: _246_
m user id: 0
owner id: 1475
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This device is a synthetic gene that generates PhaA, an enzyme that catalyzes the formation of 3-hydroxybutyryl-CoA from acetoacetyl-CoA in polyhydroxyalkanoate synthesis.
mutable notes: No special design considerations.
mutable provenance: This is a composite part.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0012
component: BBa_R0040
component: BBa_B0010
component: BBa_K156013
component: BBa_B0032
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_K156013
sequence annotation: BBa_B0032
sequence annotation: BBa_R0040
sequence: https://synbiohub.org/public/igem/BBa_K156022_sequence/1

BBa_B0012
type: Component
persistent identity: component1993821
display id: component1993821
version: 1
top level: BBa_K156022
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_R0040
type: Component
persistent identity: component1993810
display id: component1993810
version: 1
top level: BBa_K156022
access: public
definition: https://synbiohub.org/public/igem/BBa_R0040/1

BBa_B0010
type: Component
persistent identity: component1993819
display id: component1993819
version: 1
top level: BBa_K156022
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_K156013
type: Component
persistent identity: component1993818
display id: component1993818
version: 1
top level: BBa_K156022
access: public
definition: Bioplastic

BBa_B0032
type: Component
persistent identity: component1993816
display id: component1993816
version: 1
top level: BBa_K156022
access: public
definition: https://synbiohub.org/public/igem/BBa_B0032/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1993819
display id: annotation1993819
version: 1
top level: BBa_K156022
location: https://synbiohub.org/public/igem/BBa_K156022/annotation1993819/range1993819/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K156022/annotation1993819/range1993819/1
type: Range
persistent identity: range1993819
display id: range1993819
version: 1
direction: direction/forward
top level: BBa_K156022
start: 831
end: 910
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1993821
display id: annotation1993821
version: 1
top level: BBa_K156022
location: https://synbiohub.org/public/igem/BBa_K156022/annotation1993821/range1993821/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K156022/annotation1993821/range1993821/1
type: Range
persistent identity: range1993821
display id: range1993821
version: 1
direction: direction/forward
top level: BBa_K156022
start: 919
end: 959
orientation: inline

BBa_K156013
type: SequenceAnnotation
persistent identity: annotation1993818
display id: annotation1993818
version: 1
top level: BBa_K156022
location: https://synbiohub.org/public/igem/BBa_K156022/annotation1993818/range1993818/1
component: BBa_K156013

https://synbiohub.org/public/igem/BBa_K156022/annotation1993818/range1993818/1
type: Range
persistent identity: range1993818
display id: range1993818
version: 1
direction: direction/reverse
top level: BBa_K156022
start: 82
end: 822
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation1993816
display id: annotation1993816
version: 1
top level: BBa_K156022
location: https://synbiohub.org/public/igem/BBa_K156022/annotation1993816/range1993816/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_K156022/annotation1993816/range1993816/1
type: Range
persistent identity: range1993816
display id: range1993816
version: 1
direction: direction/forward
top level: BBa_K156022
start: 63
end: 75
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1993810
display id: annotation1993810
version: 1
top level: BBa_K156022
location: https://synbiohub.org/public/igem/BBa_K156022/annotation1993810/range1993810/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_K156022/annotation1993810/range1993810/1
type: Range
persistent identity: range1993810
display id: range1993810
version: 1
direction: direction/forward
top level: BBa_K156022
start: 1
end: 54
orientation: inline

BBa_K759009
type: ComponentDefinition
persistent identity: BBa K759009
display id: BBa_K759009
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS-phaC-RBS-phaA-RBS-phaB
creator: Ami Kawahata
created: 2012-09-19T11:00:00Z
modified: 2015-05-08T01:13:13Z
discontinued: false
dominant: false
group u list: _1010_
m user id: 0
owner id: 11723
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This is a construct of an P3HB (bio plastic) producing operon. RBS(BBa_b0034) is assembled on each phaC, phaA, phaB gene.
mutable notes: Nothing to remark.
mutable provenance: phaA and phaB gene is cloned from pGEM phbCAB operon which carries Ralstronia Eutropha gene.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_B0034
component: BBa_K759005
component: BBa_K342001
component: BBa_K759004
sequence annotation: BBa_K342001
sequence annotation: BBa_B0034
sequence annotation: BBa_K759004
sequence annotation: BBa_K759005
sequence: https://synbiohub.org/public/igem/BBa_K759009_sequence/1

BBa_B0034
type: Component
persistent identity: component2188341
display id: component2188341
version: 1
top level: BBa_K759009
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0034
type: Component
persistent identity: component2188344
display id: component2188344
version: 1
top level: BBa_K759009
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0034
type: Component
persistent identity: component2188347
display id: component2188347
version: 1
top level: BBa_K759009
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K759005
type: Component
persistent identity: component2188348
display id: component2188348
version: 1
top level: BBa_K759009
access: public
definition: BBa_K759005

BBa_K342001
type: Component
persistent identity: component2188342
display id: component2188342
version: 1
top level: BBa_K759009
access: public
definition: https://synbiohub.org/public/igem/BBa_K342001/1

BBa_K759004
type: Component
persistent identity: component2188345
display id: component2188345
version: 1
top level: BBa_K759009
access: public
definition: BBa_K759004

BBa_K342001
type: SequenceAnnotation
persistent identity: annotation2188342
display id: annotation2188342
version: 1
top level: BBa_K759009
location: https://synbiohub.org/public/igem/BBa_K759009/annotation2188342/range2188342/1
component: BBa_K342001

https://synbiohub.org/public/igem/BBa_K759009/annotation2188342/range2188342/1
type: Range
persistent identity: range2188342
display id: range2188342
version: 1
direction: direction/reverse
top level: BBa_K759009
start: 21
end: 1809
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2188341
display id: annotation2188341
version: 1
top level: BBa_K759009
location: https://synbiohub.org/public/igem/BBa_K759009/annotation2188341/range2188341/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K759009/annotation2188341/range2188341/1
type: Range
persistent identity: range2188341
display id: range2188341
version: 1
direction: direction/reverse
top level: BBa_K759009
start: 1
end: 12
orientation: inline

BBa_K759004
type: SequenceAnnotation
persistent identity: annotation2188345
display id: annotation2188345
version: 1
top level: BBa_K759009
location: https://synbiohub.org/public/igem/BBa_K759009/annotation2188345/range2188345/1
component: BBa_K759004

https://synbiohub.org/public/igem/BBa_K759009/annotation2188345/range2188345/1
type: Range
persistent identity: range2188345
display id: range2188345
version: 1
direction: direction/reverse
top level: BBa_K759009
start: 1836
end: 3017
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2188344
display id: annotation2188344
version: 1
top level: BBa_K759009
location: https://synbiohub.org/public/igem/BBa_K759009/annotation2188344/range2188344/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K759009/annotation2188344/range2188344/1
type: Range
persistent identity: range2188344
display id: range2188344
version: 1
direction: direction/reverse
top level: BBa_K759009
start: 1818
end: 1829
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2188347
display id: annotation2188347
version: 1
top level: BBa_K759009
location: https://synbiohub.org/public/igem/BBa_K759009/annotation2188347/range2188347/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K759009/annotation2188347/range2188347/1
type: Range
persistent identity: range2188347
display id: range2188347
version: 1
direction: direction/reverse
top level: BBa_K759009
start: 3026
end: 3037
orientation: inline

BBa_K759005
type: SequenceAnnotation
persistent identity: annotation2188348
display id: annotation2188348
version: 1
top level: BBa_K759009
location: https://synbiohub.org/public/igem/BBa_K759009/annotation2188348/range2188348/1
component: BBa_K759005

https://synbiohub.org/public/igem/BBa_K759009/annotation2188348/range2188348/1
type: Range
persistent identity: range2188348
display id: range2188348
version: 1
direction: direction/reverse
top level: BBa_K759009
start: 3044
end: 3781
orientation: inline

BBa_K156018
type: ComponentDefinition
persistent identity: BBa K156018
display id: BBa_K156018
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter + RBS + phaA
creator: George McArthur IV
created: 2008-10-28T12:00:00Z
modified: 2015-05-08T01:10:54Z
discontinued: false
dominant: false
experience: experience/None
group u list: _246_
m user id: 0
owner id: 1475
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This device (a synthetic gene) generates PhaA.
mutable notes: None.
mutable provenance: This is a composite part.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0032
component: BBa_K156012
component: BBa_R0040
sequence annotation: BBa_B0032
sequence annotation: BBa_R0040
sequence annotation: BBa_K156012
sequence: https://synbiohub.org/public/igem/BBa_K156018_sequence/1

BBa_B0032
type: Component
persistent identity: component1993645
display id: component1993645
version: 1
top level: BBa_K156018
access: public
definition: https://synbiohub.org/public/igem/BBa_B0032/1

BBa_K156012
type: Component
persistent identity: component1993647
display id: component1993647
version: 1
top level: BBa_K156018
access: public
definition: Bioplastic

BBa_R0040
type: Component
persistent identity: component1993639
display id: component1993639
version: 1
top level: BBa_K156018
access: public
definition: https://synbiohub.org/public/igem/BBa_R0040/1

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation1993645
display id: annotation1993645
version: 1
top level: BBa_K156018
location: https://synbiohub.org/public/igem/BBa_K156018/annotation1993645/range1993645/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_K156018/annotation1993645/range1993645/1
type: Range
persistent identity: range1993645
display id: range1993645
version: 1
direction: direction/forward
top level: BBa_K156018
start: 63
end: 75
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1993639
display id: annotation1993639
version: 1
top level: BBa_K156018
location: https://synbiohub.org/public/igem/BBa_K156018/annotation1993639/range1993639/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_K156018/annotation1993639/range1993639/1
type: Range
persistent identity: range1993639
display id: range1993639
version: 1
direction: direction/forward
top level: BBa_K156018
start: 1
end: 54
orientation: inline

BBa_K156012
type: SequenceAnnotation
persistent identity: annotation1993647
display id: annotation1993647
version: 1
top level: BBa_K156018
location: https://synbiohub.org/public/igem/BBa_K156018/annotation1993647/range1993647/1
component: BBa_K156012

https://synbiohub.org/public/igem/BBa_K156018/annotation1993647/range1993647/1
type: Range
persistent identity: range1993647
display id: range1993647
version: 1
direction: direction/reverse
top level: BBa_K156018
start: 82
end: 1263
orientation: inline

BBa_K759010
type: ComponentDefinition
persistent identity: BBa K759010
display id: BBa_K759010
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: P3HB producing construct (without a promoter)
creator: Ami Kawahata
created: 2012-09-19T11:00:00Z
modified: 2015-05-08T01:13:13Z
discontinued: false
dominant: false
group u list: _1010_
m user id: 0
owner id: 11723
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This is an construct for P3HB (bio plastic). It lacks the promoter for the users that wants to try other kinds of promoters.
mutable notes: Nothing to remark.
mutable provenance: phaA and phaB was cloned from pGEM phbCAB which carries the gene of Ralstonia Eutropha.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K759005
component: BBa_B0015
component: BBa_B0034
component: BBa_K342001
component: BBa_K759004
sequence annotation: BBa_B0034
sequence annotation: BBa_K759005
sequence annotation: BBa_K759004
sequence annotation: BBa_K342001
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_K759010_sequence/1

BBa_K759005
type: Component
persistent identity: component2188428
display id: component2188428
version: 1
top level: BBa_K759010
access: public
definition: BBa_K759005

BBa_B0015
type: Component
persistent identity: component2188435
display id: component2188435
version: 1
top level: BBa_K759010
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_B0034
type: Component
persistent identity: component2188427
display id: component2188427
version: 1
top level: BBa_K759010
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0034
type: Component
persistent identity: component2188421
display id: component2188421
version: 1
top level: BBa_K759010
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0034
type: Component
persistent identity: component2188424
display id: component2188424
version: 1
top level: BBa_K759010
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K342001
type: Component
persistent identity: component2188422
display id: component2188422
version: 1
top level: BBa_K759010
access: public
definition: https://synbiohub.org/public/igem/BBa_K342001/1

BBa_K759004
type: Component
persistent identity: component2188425
display id: component2188425
version: 1
top level: BBa_K759010
access: public
definition: BBa_K759004

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2188424
display id: annotation2188424
version: 1
top level: BBa_K759010
location: https://synbiohub.org/public/igem/BBa_K759010/annotation2188424/range2188424/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K759010/annotation2188424/range2188424/1
type: Range
persistent identity: range2188424
display id: range2188424
version: 1
direction: direction/reverse
top level: BBa_K759010
start: 1818
end: 1829
orientation: inline

BBa_K759005
type: SequenceAnnotation
persistent identity: annotation2188428
display id: annotation2188428
version: 1
top level: BBa_K759010
location: https://synbiohub.org/public/igem/BBa_K759010/annotation2188428/range2188428/1
component: BBa_K759005

https://synbiohub.org/public/igem/BBa_K759010/annotation2188428/range2188428/1
type: Range
persistent identity: range2188428
display id: range2188428
version: 1
direction: direction/reverse
top level: BBa_K759010
start: 3044
end: 3781
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2188427
display id: annotation2188427
version: 1
top level: BBa_K759010
location: https://synbiohub.org/public/igem/BBa_K759010/annotation2188427/range2188427/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K759010/annotation2188427/range2188427/1
type: Range
persistent identity: range2188427
display id: range2188427
version: 1
direction: direction/reverse
top level: BBa_K759010
start: 3026
end: 3037
orientation: inline

BBa_K759004
type: SequenceAnnotation
persistent identity: annotation2188425
display id: annotation2188425
version: 1
top level: BBa_K759010
location: https://synbiohub.org/public/igem/BBa_K759010/annotation2188425/range2188425/1
component: BBa_K759004

https://synbiohub.org/public/igem/BBa_K759010/annotation2188425/range2188425/1
type: Range
persistent identity: range2188425
display id: range2188425
version: 1
direction: direction/reverse
top level: BBa_K759010
start: 1836
end: 3017
orientation: inline

BBa_K342001
type: SequenceAnnotation
persistent identity: annotation2188422
display id: annotation2188422
version: 1
top level: BBa_K759010
location: https://synbiohub.org/public/igem/BBa_K759010/annotation2188422/range2188422/1
component: BBa_K342001

https://synbiohub.org/public/igem/BBa_K759010/annotation2188422/range2188422/1
type: Range
persistent identity: range2188422
display id: range2188422
version: 1
direction: direction/reverse
top level: BBa_K759010
start: 21
end: 1809
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2188435
display id: annotation2188435
version: 1
top level: BBa_K759010
location: https://synbiohub.org/public/igem/BBa_K759010/annotation2188435/range2188435/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K759010/annotation2188435/range2188435/1
type: Range
persistent identity: range2188435
display id: range2188435
version: 1
direction: direction/reverse
top level: BBa_K759010
start: 3790
end: 3918
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2188421
display id: annotation2188421
version: 1
top level: BBa_K759010
location: https://synbiohub.org/public/igem/BBa_K759010/annotation2188421/range2188421/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K759010/annotation2188421/range2188421/1
type: Range
persistent identity: range2188421
display id: range2188421
version: 1
direction: direction/reverse
top level: BBa_K759010
start: 1
end: 12
orientation: inline

BBa_K759005
type: ComponentDefinition
persistent identity: BBa K759005
display id: BBa_K759005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: phaB (acetoacetyl-CoA reductase orgin from Ralstonia eutropha)
creator: Ami Kawahata
created: 2012-09-18T11:00:00Z
modified: 2015-05-08T01:13:13Z
discontinued: false
dominant: false
group u list: _1010_
m user id: 0
owner id: 11723
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: PhaB codes ???acetoacetyl-CoA reductase??? which catalyzes the reduction of acetoacetyl-CoA into (R)-3-hydroxybutyryl-CoA (3HB-CoA). This is an enzyme that catalyzes the second step of P3(HB) producing pathway.
mutable notes: We cloned the gene by constructing primers.
mutable provenance: This gene was cloned from pGEM phbCAB. The pGEM phbCAB carries the synthetic operon genes (phaA,phaB,phaC) of Ralstonia eutropha.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K759005_sequence/1

BBa_K125501
type: ComponentDefinition
persistent identity: BBa K125501
display id: BBa_K125501
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: phaA BioPlastic polyhydroxybutyrate synthesis pathway (origin PCC6803 slr1994)
creator: Norman Wang
created: 2008-08-09T11:00:00Z
modified: 2015-05-08T01:09:45Z
discontinued: false
dominant: false
experience: experience/None
group u list: _179_
m user id: 0
owner id: 2680
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: phaA
mutable notes: PHB synthesis pathway
mutable provenance: Synechocystis sp. PCC6803
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: start
sequence annotation: mt tGa to tAa
sequence annotation: inserted second stop
sequence: https://synbiohub.org/public/igem/BBa_K125501_sequence/1

start
type: SequenceAnnotation
persistent identity: annotation1971707
display id: annotation1971707
version: 1
top level: BBa_K125501
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K125501/annotation1971707/range1971707/1

https://synbiohub.org/public/igem/BBa_K125501/annotation1971707/range1971707/1
type: Range
persistent identity: range1971707
display id: range1971707
version: 1
direction: direction/forward
top level: BBa_K125501
start: 1
end: 3
orientation: inline

mt tGa to tAa
type: SequenceAnnotation
persistent identity: annotation1971705
display id: annotation1971705
version: 1
top level: BBa_K125501
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K125501/annotation1971705/range1971705/1

https://synbiohub.org/public/igem/BBa_K125501/annotation1971705/range1971705/1
type: Range
persistent identity: range1971705
display id: range1971705
version: 1
direction: direction/forward
top level: BBa_K125501
start: 1228
end: 1230
orientation: inline

inserted second stop
type: SequenceAnnotation
persistent identity: annotation1971706
display id: annotation1971706
version: 1
top level: BBa_K125501
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K125501/annotation1971706/range1971706/1

https://synbiohub.org/public/igem/BBa_K125501/annotation1971706/range1971706/1
type: Range
persistent identity: range1971706
display id: range1971706
version: 1
direction: direction/forward
top level: BBa_K125501
start: 1231
end: 1233
orientation: inline

BBa_K338024
type: ComponentDefinition
persistent identity: BBa K338024
display id: BBa_K338024
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Plastic Intermediate: phaC1 + Terminator
creator: Lucas Hartsough
created: 2010-10-26T11:00:00Z
modified: 2015-05-08T01:12:07Z
discontinued: false
dominant: false
group u list: _454_
m user id: 0
owner id: 6013
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: A construction intermediate used to produce <partinfo>BBa_K338004</partinfo>. Contains the third of three genes required to synthesize polyhydroxybutyrate (PHB).
mutable notes: Designed to be ligated downstream of <partinfo>K338022</partinfo> and <partinfo>K338023</partinfo>.
mutable provenance: The Registry of Standard Biological Parts
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K156014
component: BBa_B0015
sequence annotation: BBa_K156014
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_K338024_sequence/1

BBa_K156014
type: Component
persistent identity: component2104854
display id: component2104854
version: 1
top level: BBa_K338024
access: public
definition: Bioplastic

BBa_B0015
type: Component
persistent identity: component2104861
display id: component2104861
version: 1
top level: BBa_K338024
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_K156014
type: SequenceAnnotation
persistent identity: annotation2104854
display id: annotation2104854
version: 1
top level: BBa_K338024
location: https://synbiohub.org/public/igem/BBa_K338024/annotation2104854/range2104854/1
component: BBa_K156014

https://synbiohub.org/public/igem/BBa_K338024/annotation2104854/range2104854/1
type: Range
persistent identity: range2104854
display id: range2104854
version: 1
direction: direction/reverse
top level: BBa_K338024
start: 1
end: 1349
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2104861
display id: annotation2104861
version: 1
top level: BBa_K338024
location: https://synbiohub.org/public/igem/BBa_K338024/annotation2104861/range2104861/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K338024/annotation2104861/range2104861/1
type: Range
persistent identity: range2104861
display id: range2104861
version: 1
direction: direction/reverse
top level: BBa_K338024
start: 1358
end: 1486
orientation: inline

BBa_K338003
type: ComponentDefinition
persistent identity: BBa K338003
display id: BBa_K338003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PHA Synthase Composite, Part 1/2
creator: Caltech iGEM 2010
created: 2010-10-25T11:00:00Z
modified: 2015-05-08T01:12:07Z
discontinued: false
dominant: false
experience: experience/None
group u list: _454_
m user id: 0
owner id: 6013
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This is half of a planned part which would contain all three PHA synthase genes required to produce [http://en.wikipedia.org/wiki/Polyhydroxybutyrate polyhydroxybutyrate] (PHB) in cells: ''phaA'', ''phaB1'', ''phaC1''. This half contains only ''phaA'': <partinfo>BBa_K156012</partinfo>. It was designed to be ligated upstream of part BBa_K338004.
mutable notes: When ligated upstream of BBa_K338004, the completed construct was designed to express all three PHA synthase genes required to make PHB oligomers from soybean oil. The three genes would be transcribed polycistronically on a single mRNA transcript under the IPTG-inducible control of the <partinfo>BBa_K215000</partinfo> promoter. Naturally, each gene is preceded by a standard RBS (<partinfo>B0034</partinfo>) and the transcript finishes with a strong terminator (<partinfo>B0015</partinfo>), for a total size of about 3500bp. Note that these three genes should only cause the production of PHB ''oligomers'' in cells, not hardened plastic. A crosslinking agent is required to link the oligomers and form the final plastic product. Over-expression of the ''phaC1'' gene could cause some crosslinking, but this has not been experimentally verified.
mutable provenance: Registry of Standard Biological Parts
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K156012
component: BBa_B0034
component: BBa_K215000
sequence annotation: BBa_B0034
sequence annotation: BBa_K156012
sequence annotation: BBa_K215000
sequence: https://synbiohub.org/public/igem/BBa_K338003_sequence/1

BBa_K156012
type: Component
persistent identity: component2101446
display id: component2101446
version: 1
top level: BBa_K338003
access: public
definition: Bioplastic

BBa_B0034
type: Component
persistent identity: component2101445
display id: component2101445
version: 1
top level: BBa_K338003
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0034
type: Component
persistent identity: component2101448
display id: component2101448
version: 1
top level: BBa_K338003
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K215000
type: Component
persistent identity: component2101443
display id: component2101443
version: 1
top level: BBa_K338003
access: public
definition: https://synbiohub.org/public/igem/BBa_K215000/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2101448
display id: annotation2101448
version: 1
top level: BBa_K338003
location: https://synbiohub.org/public/igem/BBa_K338003/annotation2101448/range2101448/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K338003/annotation2101448/range2101448/1
type: Range
persistent identity: range2101448
display id: range2101448
version: 1
direction: direction/reverse
top level: BBa_K338003
start: 1292
end: 1303
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2101445
display id: annotation2101445
version: 1
top level: BBa_K338003
location: https://synbiohub.org/public/igem/BBa_K338003/annotation2101445/range2101445/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K338003/annotation2101445/range2101445/1
type: Range
persistent identity: range2101445
display id: range2101445
version: 1
direction: direction/reverse
top level: BBa_K338003
start: 84
end: 95
orientation: inline

BBa_K156012
type: SequenceAnnotation
persistent identity: annotation2101446
display id: annotation2101446
version: 1
top level: BBa_K338003
location: https://synbiohub.org/public/igem/BBa_K338003/annotation2101446/range2101446/1
component: BBa_K156012

https://synbiohub.org/public/igem/BBa_K338003/annotation2101446/range2101446/1
type: Range
persistent identity: range2101446
display id: range2101446
version: 1
direction: direction/reverse
top level: BBa_K338003
start: 102
end: 1283
orientation: inline

BBa_K215000
type: SequenceAnnotation
persistent identity: annotation2101443
display id: annotation2101443
version: 1
top level: BBa_K338003
location: https://synbiohub.org/public/igem/BBa_K338003/annotation2101443/range2101443/1
component: BBa_K215000

https://synbiohub.org/public/igem/BBa_K338003/annotation2101443/range2101443/1
type: Range
persistent identity: range2101443
display id: range2101443
version: 1
direction: direction/reverse
top level: BBa_K338003
start: 1
end: 75
orientation: inline

Bioplastic
type: ComponentDefinition
persistent identity: BBa K156012
display id: BBa_K156012
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: phaA (acetyl-CoA acetyltransferase)
creator: George McArthur IV
created: 2008-10-28T12:00:00Z
modified: 2015-05-08T01:10:54Z
discontinued: false
dominant: false
experience: experience/None
group u list: _246_
m user id: 0
owner id: 1475
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part codes for an enzyme that catalyzes the synthesis of acetoacetyl-coenzyme A from two molecules of acetyl-coenzyme A. The enzyme can also act as a thiolase, catalyzing the reverse reaction and generating two-carbon units from the four-carbon product of fatty acid oxidation.
mutable notes: This part was codon-optimized for expression in E. coli. The following restriction enzyme sites were also removed: EcoRI, NotI, XbaI, SpeI, PstI, NheI, AvrII, ApoI, MfeI, NsiI, SbfI.
mutable provenance: This coding region is native to ''Ralstonia eutropha'' H16. The original sequence was obtained from GenBank (VERSION NC_008313.1, GI:113866031).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K156012_sequence/1

BBa_K156016
type: ComponentDefinition
persistent identity: BBa K156016
display id: BBa_K156016
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS + phaB1
creator: George McArthur IV
created: 2008-10-28T12:00:00Z
modified: 2015-05-08T01:10:54Z
discontinued: false
dominant: false
experience: experience/None
group u list: _246_
m user id: 0
owner id: 1475
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This composite part is made of a standard ribosomal binding site and a part that codes for an enzyme that catalyzes the formation of 3-hydroxybutyryl-CoA from acetoacetyl-CoA in polyhydroxyalkanoate synthesis.
mutable notes: None.
mutable provenance: This is a composite part.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_B0032
component: BBa_K156013
sequence annotation: BBa_B0032
sequence annotation: BBa_K156013
sequence: https://synbiohub.org/public/igem/BBa_K156016_sequence/1

BBa_B0032
type: Component
persistent identity: component1993477
display id: component1993477
version: 1
top level: BBa_K156016
access: public
definition: https://synbiohub.org/public/igem/BBa_B0032/1

BBa_K156013
type: Component
persistent identity: component1993479
display id: component1993479
version: 1
top level: BBa_K156016
access: public
definition: Bioplastic

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation1993477
display id: annotation1993477
version: 1
top level: BBa_K156016
location: https://synbiohub.org/public/igem/BBa_K156016/annotation1993477/range1993477/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_K156016/annotation1993477/range1993477/1
type: Range
persistent identity: range1993477
display id: range1993477
version: 1
direction: direction/forward
top level: BBa_K156016
start: 1
end: 13
orientation: inline

BBa_K156013
type: SequenceAnnotation
persistent identity: annotation1993479
display id: annotation1993479
version: 1
top level: BBa_K156016
location: https://synbiohub.org/public/igem/BBa_K156016/annotation1993479/range1993479/1
component: BBa_K156013

https://synbiohub.org/public/igem/BBa_K156016/annotation1993479/range1993479/1
type: Range
persistent identity: range1993479
display id: range1993479
version: 1
direction: direction/reverse
top level: BBa_K156016
start: 20
end: 760
orientation: inline

BBa_K759007
type: ComponentDefinition
persistent identity: BBa K759007
display id: BBa_K759007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS(B0034)and phaB (acetoacetyl-CoA reductase orgin from Ralstonia eutropha)
creator: Ami Kawahata
created: 2012-09-19T11:00:00Z
modified: 2015-05-08T01:13:13Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1010_
m user id: 0
owner id: 11723
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: A composite of RBS(BBa_B0034) and phaB (acetoacetyl-CoA reductase orgin from Ralstonia eutropha) BBa_K759005.
mutable notes: Nothing to remark.
mutable provenance: phAB(BBa_K750005) was cloned from pGEM phbCAB which carries the synthetic operon genes (phaA,phaB,phaC) of Ralstonia eutropha.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_B0034
component: BBa_K759005
sequence annotation: BBa_K759005
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K759007_sequence/1

BBa_B0034
type: Component
persistent identity: component2187522
display id: component2187522
version: 1
top level: BBa_K759007
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K759005
type: Component
persistent identity: component2187523
display id: component2187523
version: 1
top level: BBa_K759007
access: public
definition: BBa_K759005

BBa_K759005
type: SequenceAnnotation
persistent identity: annotation2187523
display id: annotation2187523
version: 1
top level: BBa_K759007
location: https://synbiohub.org/public/igem/BBa_K759007/annotation2187523/range2187523/1
component: BBa_K759005

https://synbiohub.org/public/igem/BBa_K759007/annotation2187523/range2187523/1
type: Range
persistent identity: range2187523
display id: range2187523
version: 1
direction: direction/reverse
top level: BBa_K759007
start: 19
end: 756
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2187522
display id: annotation2187522
version: 1
top level: BBa_K759007
location: https://synbiohub.org/public/igem/BBa_K759007/annotation2187522/range2187522/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K759007/annotation2187522/range2187522/1
type: Range
persistent identity: range2187522
display id: range2187522
version: 1
direction: direction/reverse
top level: BBa_K759007
start: 1
end: 12
orientation: inline

BBa_K156015
type: ComponentDefinition
persistent identity: BBa K156015
display id: BBa_K156015
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS + phaA
creator: George McArthur IV
created: 2008-10-28T12:00:00Z
modified: 2015-05-08T01:10:54Z
discontinued: false
dominant: false
experience: experience/None
group u list: _246_
m user id: 0
owner id: 1475
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This part is a composite part made of a standard ribosomal binding site and a second part that codes for an acetyl-CoA acetyltransferase (PhaA).
mutable notes: None.
mutable provenance: This is a composite part made from existing BioBricks.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_B0032
component: BBa_K156012
sequence annotation: BBa_B0032
sequence annotation: BBa_K156012
sequence: https://synbiohub.org/public/igem/BBa_K156015_sequence/1

BBa_B0032
type: Component
persistent identity: component1993440
display id: component1993440
version: 1
top level: BBa_K156015
access: public
definition: https://synbiohub.org/public/igem/BBa_B0032/1

BBa_K156012
type: Component
persistent identity: component1993442
display id: component1993442
version: 1
top level: BBa_K156015
access: public
definition: Bioplastic

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation1993440
display id: annotation1993440
version: 1
top level: BBa_K156015
location: https://synbiohub.org/public/igem/BBa_K156015/annotation1993440/range1993440/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_K156015/annotation1993440/range1993440/1
type: Range
persistent identity: range1993440
display id: range1993440
version: 1
direction: direction/forward
top level: BBa_K156015
start: 1
end: 13
orientation: inline

BBa_K156012
type: SequenceAnnotation
persistent identity: annotation1993442
display id: annotation1993442
version: 1
top level: BBa_K156015
location: https://synbiohub.org/public/igem/BBa_K156015/annotation1993442/range1993442/1
component: BBa_K156012

https://synbiohub.org/public/igem/BBa_K156015/annotation1993442/range1993442/1
type: Range
persistent identity: range1993442
display id: range1993442
version: 1
direction: direction/reverse
top level: BBa_K156015
start: 20
end: 1201
orientation: inline

BBa_K759004
type: ComponentDefinition
persistent identity: BBa K759004
display id: BBa_K759004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: phaA (beta-ketothiolase orgin from Ralstonia eutropha)
creator: Ami Kawahata
created: 2012-09-18T11:00:00Z
modified: 2015-05-08T01:13:13Z
discontinued: false
dominant: false
group u list: _1010_
m user id: 0
owner id: 11723
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: PhaA codes ???Beta- ketothiolase (acetyl-CoA acetyltransferase)???which catalyzes the conversion of two acetyl-CoA molecules into acetoacetyl-CoA. It is an enzyme that catalyzes the first step of P3(HB) producing pathway.
mutable notes: In the pGEM phbCAB phaA, we found a mutation in the 00th base according to the data base of NCBI. However, it is confirmed that pGEM phbCAB transformed E.Coli produces P3HB.
mutable provenance: This gene was cloned from pGEM phbCAB. The pGEM phbCAB carries the synthetic operon genes (phaA,phaB,phaC) of Ralstonia eutropha.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K759004_sequence/1