//function/celldeath
type: Collection
persistent identity: function celldeath
display id: function_celldeath
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
owned by: james
owned by: myers
member: BBa_K112306
member: Lysis
member: BBa_K404113
member: BBa_K864150
member: BBa_K112017
member: BBa_K112021
member: BBa_K112301
member: BBa_K733012
member: BBa_K112310
member: BBa_K258002
member: BBa_K1054001
member: BBa_K112000
member: BBa_K117000
member: BBa_K112807
member: BBa_K112802
member: BBa_K112005
member: BBa_K358023
member: gpS
member: ccdB
member: BBa_K112315
member: BBa_K2040122
member: BBa_K404111
member: BBa_K112014
member: BBa_K1467203
member: BBa_K1631012
member: BBa_K1631025
member: BBa_K112002
member: BBa_K112317
member: toxin ParE
member: BBa_K112304
member: BBa_K112303
member: BBa_K112316
member: BBa_K358019
member: BBa_K112015
member: BBa_K137131
member: BBa_K112016
member: Holin, T4
member: BBa_K112201
member: BBa_K1631018
member: BBa_K1631024
member: BBa_K112305
member: BBa_K748006
member: CcdB
member: BBa_K1631023
member: BBa_K404109
member: BBa_K112009
member: BBa_K112004
member: BBa_K112314
member: BBa_K772101
member: BBa_K112020
member: BBa_K515106
member: BBa_K1631022
member: BBa_K112013
member: BBa_K112307
member: BBa_K404112
member: BBa_K112302
member: BBa_K533012
member: BBa_K112022
member: BBa_K2110008
member: BBa_K1054002
member: BBa_K112018
member: BBa_K1184000
member: BBa_K112311
member: BBa_K112806
member: BBa_K112023
member: BBa_K112010
member: BBa_K2110004
member: BBa_K108001
member: BBa_K112007
member: BBa_K112006
member: BBa_K112019
member: BBa_K112308

BBa_K1631003
type: ComponentDefinition
persistent identity: BBa K1631003
display id: BBa_K1631003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Tlanslational unit of Colicin Lysis Protein (for colicin-E3)
creator: Yuto Yamanaka
created: 2015-09-12T11:00:00Z
modified: 2015-09-18T06:28:24Z
discontinued: false
dominant: false
group u list: _2048_
m user id: 23567
owner id: 23567
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Colicin lysis protein allows colicins to be released. The mechanism how the colicin lysis protein allows colicin release has not been fully elucidated, but it is sure that this protein raise membrane permiability and cause quasilysis.[1] Colicins are a cytotoxins which are released to environment and kill other related strains.
mutable notes: none
mutable provenance: pColE3-CA38
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
sequence annotation: BBa_B0034
sequence annotation: Colicin Lysis Protein
sequence: https://synbiohub.org/public/igem/BBa_K1631003_sequence/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2453045
display id: annotation2453045
version: 1
top level: BBa_K1631003
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1631003/annotation2453045/range2453045/1

https://synbiohub.org/public/igem/BBa_K1631003/annotation2453045/range2453045/1
type: Range
persistent identity: range2453045
display id: range2453045
version: 1
direction: direction/forward
top level: BBa_K1631003
start: 1
end: 12
orientation: inline

Colicin Lysis Protein
type: SequenceAnnotation
persistent identity: annotation2453046
display id: annotation2453046
version: 1
top level: BBa_K1631003
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1631003/annotation2453046/range2453046/1

https://synbiohub.org/public/igem/BBa_K1631003/annotation2453046/range2453046/1
type: Range
persistent identity: range2453046
display id: range2453046
version: 1
direction: direction/forward
top level: BBa_K1631003
start: 13
end: 156
orientation: inline

BBa_K258002
type: ComponentDefinition
persistent identity: BBa K258002
display id: BBa_K258002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Granulysin, a T Cell Product,Kills Bacteria by Altering Membrane Permeability
creator: Ozkan IS
created: 2009-10-02T11:00:00Z
modified: 2016-02-01T12:59:26Z
discontinued: false
dominant: true
experience: experience/None
group u list: _358_
m user id: 4206
owner id: 4260
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: Granulysin, a protein located in the acidic granules of human NK cells and cytotoxic T cells, has microbial activity against a broad spectrum of microbial pathogens. The substance released by cytotoxic T cells (CD8) when attached to infected body cells. It creates holes in the target cell membrane and destroy it. That is,Granulysin induces apoptosis in target cells and have antimicrobial action. It is broadly antimicrobial, killing microbes that cause, for example, tuberculosis and malaria, and can destroy some tumors. A series of peptides generated from the amino acid sequence of granulysin are potential antibiotics. Granulysin increased the permeability of bacterial membranes, as judged by its ability to allow access of cytosolic ??-galactosidase to its impermeant substrate.Granulysin functions to create holes in the target cell membrane and destroy it. Granulysin is able to induce apoptosis in target cells and also has antimicrobial action.
mutable notes: Granulysin can be purified via nickel affinity chromatography.
mutable provenance: Granulysin is a member of the saposin-like protein (SAPLIP) family. The gene was synthesized at GENEART in pMA vector
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K258002_sequence/1

Holin, T4
type: ComponentDefinition
persistent identity: BBa K112003
display id: BBa_K112003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: T4 holin, without start or stop codon, berkeley standard
creator: Bing Xia
created: 2008-09-02T11:00:00Z
modified: 2015-05-08T01:09:13Z
discontinued: false
dominant: false
experience: experience/None
group u list: _224_
m user id: 0
owner id: 2998
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: T4 holin gene without start or stop codons, in BBb format. ---- This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at: [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
mutable provenance: PCR bx3/bx4 on Bjh1301 (682 bp, EcoRI/BamHI/DpnI) Sub into pBca1256 (EcoRI/BamHI) Product is pBca1256-K112003 ---------------------------------------- bx3 Forward Biobricking of {<T4 Holin>} ccagtGAATTCatgAGATCTgcagcacctagaatatcattttcgccc bx4 Reverse Biobricking of {<T4 Holin>} cgttaGGATCCtttagcccttcctaatattctggcc
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence: https://synbiohub.org/public/igem/BBa_K112003_sequence/1

BBa_K112020
type: ComponentDefinition
persistent identity: BBa K112020
display id: BBa_K112020
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Lambda phage lysis device
creator: Bing Xia
created: 2008-10-23T11:00:00Z
modified: 2015-05-08T01:09:13Z
discontinued: false
dominant: false
group u list: _224_
m user id: 0
owner id: 2998
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Lysozyme and holin production are driven by pBad, and antiholin is driven by PconC6.
mutable provenance: Assembled using 2 antibiotic assembly from basic parts.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_K112305
component: BBa_K112999
component: BBa_K112903
component: BBa_K112317
component: BBa_K112710
component: BBa_K112311
component: BBa_K112906
component: BBa_K112900
sequence annotation: BBa_K112999
sequence annotation: BBa_K112900
sequence annotation: BBa_K112710
sequence annotation: BBa_K112906
sequence annotation: BBa_K112903
sequence annotation: BBa_K112305
sequence annotation: BBa_K112311
sequence annotation: BBa_K112317
sequence: https://synbiohub.org/public/igem/BBa_K112020_sequence/1

BBa_K112305
type: Component
persistent identity: component1986749
display id: component1986749
version: 1
top level: BBa_K112020
access: public
definition: BBa_K112305

BBa_K112999
type: Component
persistent identity: component1986754
display id: component1986754
version: 1
top level: BBa_K112020
access: public
definition: https://synbiohub.org/public/igem/BBa_K112999/1

BBa_K112903
type: Component
persistent identity: component1986753
display id: component1986753
version: 1
top level: BBa_K112020
access: public
definition: https://synbiohub.org/public/igem/BBa_K112903/1

BBa_K112317
type: Component
persistent identity: component1986757
display id: component1986757
version: 1
top level: BBa_K112020
access: public
definition: BBa_K112317

BBa_K112999
type: Component
persistent identity: component1986756
display id: component1986756
version: 1
top level: BBa_K112020
access: public
definition: https://synbiohub.org/public/igem/BBa_K112999/1

BBa_K112999
type: Component
persistent identity: component1986748
display id: component1986748
version: 1
top level: BBa_K112020
access: public
definition: https://synbiohub.org/public/igem/BBa_K112999/1

BBa_K112999
type: Component
persistent identity: component1986750
display id: component1986750
version: 1
top level: BBa_K112020
access: public
definition: https://synbiohub.org/public/igem/BBa_K112999/1

BBa_K112999
type: Component
persistent identity: component1986758
display id: component1986758
version: 1
top level: BBa_K112020
access: public
definition: https://synbiohub.org/public/igem/BBa_K112999/1

BBa_K112710
type: Component
persistent identity: component1986759
display id: component1986759
version: 1
top level: BBa_K112020
access: public
definition: https://synbiohub.org/public/igem/BBa_K112710/1

BBa_K112311
type: Component
persistent identity: component1986751
display id: component1986751
version: 1
top level: BBa_K112020
access: public
definition: BBa_K112311

BBa_K112999
type: Component
persistent identity: component1986752
display id: component1986752
version: 1
top level: BBa_K112020
access: public
definition: https://synbiohub.org/public/igem/BBa_K112999/1

BBa_K112906
type: Component
persistent identity: component1986755
display id: component1986755
version: 1
top level: BBa_K112020
access: public
definition: https://synbiohub.org/public/igem/BBa_K112906/1

BBa_K112900
type: Component
persistent identity: component1986747
display id: component1986747
version: 1
top level: BBa_K112020
access: public
definition: https://synbiohub.org/public/igem/BBa_K112900/1

BBa_K112999
type: SequenceAnnotation
persistent identity: annotation1986756
display id: annotation1986756
version: 1
top level: BBa_K112020
location: https://synbiohub.org/public/igem/BBa_K112020/annotation1986756/range1986756/1
component: BBa_K112999

https://synbiohub.org/public/igem/BBa_K112020/annotation1986756/range1986756/1
type: Range
persistent identity: range1986756
display id: range1986756
version: 1
direction: direction/reverse
top level: BBa_K112020
start: 2260
end: 2265
orientation: inline

BBa_K112900
type: SequenceAnnotation
persistent identity: annotation1986747
display id: annotation1986747
version: 1
top level: BBa_K112020
location: https://synbiohub.org/public/igem/BBa_K112020/annotation1986747/range1986747/1
component: BBa_K112900

https://synbiohub.org/public/igem/BBa_K112020/annotation1986747/range1986747/1
type: Range
persistent identity: range1986747
display id: range1986747
version: 1
direction: direction/reverse
top level: BBa_K112020
start: 1
end: 1225
orientation: inline

BBa_K112710
type: SequenceAnnotation
persistent identity: annotation1986759
display id: annotation1986759
version: 1
top level: BBa_K112020
location: https://synbiohub.org/public/igem/BBa_K112020/annotation1986759/range1986759/1
component: BBa_K112710

https://synbiohub.org/public/igem/BBa_K112020/annotation1986759/range1986759/1
type: Range
persistent identity: range1986759
display id: range1986759
version: 1
direction: direction/reverse
top level: BBa_K112020
start: 2616
end: 2730
orientation: inline

BBa_K112999
type: SequenceAnnotation
persistent identity: annotation1986748
display id: annotation1986748
version: 1
top level: BBa_K112020
location: https://synbiohub.org/public/igem/BBa_K112020/annotation1986748/range1986748/1
component: BBa_K112999

https://synbiohub.org/public/igem/BBa_K112020/annotation1986748/range1986748/1
type: Range
persistent identity: range1986748
display id: range1986748
version: 1
direction: direction/reverse
top level: BBa_K112020
start: 1226
end: 1231
orientation: inline

BBa_K112999
type: SequenceAnnotation
persistent identity: annotation1986750
display id: annotation1986750
version: 1
top level: BBa_K112020
location: https://synbiohub.org/public/igem/BBa_K112020/annotation1986750/range1986750/1
component: BBa_K112999

https://synbiohub.org/public/igem/BBa_K112020/annotation1986750/range1986750/1
type: Range
persistent identity: range1986750
display id: range1986750
version: 1
direction: direction/reverse
top level: BBa_K112020
start: 1728
end: 1733
orientation: inline

BBa_K112999
type: SequenceAnnotation
persistent identity: annotation1986758
display id: annotation1986758
version: 1
top level: BBa_K112020
location: https://synbiohub.org/public/igem/BBa_K112020/annotation1986758/range1986758/1
component: BBa_K112999

https://synbiohub.org/public/igem/BBa_K112020/annotation1986758/range1986758/1
type: Range
persistent identity: range1986758
display id: range1986758
version: 1
direction: direction/reverse
top level: BBa_K112020
start: 2610
end: 2615
orientation: inline

BBa_K112906
type: SequenceAnnotation
persistent identity: annotation1986755
display id: annotation1986755
version: 1
top level: BBa_K112020
location: https://synbiohub.org/public/igem/BBa_K112020/annotation1986755/range1986755/1
component: BBa_K112906

https://synbiohub.org/public/igem/BBa_K112020/annotation1986755/range1986755/1
type: Range
persistent identity: range1986755
display id: range1986755
version: 1
direction: direction/reverse
top level: BBa_K112020
start: 2219
end: 2259
orientation: inline

BBa_K112903
type: SequenceAnnotation
persistent identity: annotation1986753
display id: annotation1986753
version: 1
top level: BBa_K112020
location: https://synbiohub.org/public/igem/BBa_K112020/annotation1986753/range1986753/1
component: BBa_K112903

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type: Range
persistent identity: range1986753
display id: range1986753
version: 1
direction: direction/reverse
top level: BBa_K112020
start: 2078
end: 2212
orientation: inline

BBa_K112999
type: SequenceAnnotation
persistent identity: annotation1986752
display id: annotation1986752
version: 1
top level: BBa_K112020
location: https://synbiohub.org/public/igem/BBa_K112020/annotation1986752/range1986752/1
component: BBa_K112999

https://synbiohub.org/public/igem/BBa_K112020/annotation1986752/range1986752/1
type: Range
persistent identity: range1986752
display id: range1986752
version: 1
direction: direction/reverse
top level: BBa_K112020
start: 2072
end: 2077
orientation: inline

BBa_K112305
type: SequenceAnnotation
persistent identity: annotation1986749
display id: annotation1986749
version: 1
top level: BBa_K112020
location: https://synbiohub.org/public/igem/BBa_K112020/annotation1986749/range1986749/1
component: BBa_K112305

https://synbiohub.org/public/igem/BBa_K112020/annotation1986749/range1986749/1
type: Range
persistent identity: range1986749
display id: range1986749
version: 1
direction: direction/reverse
top level: BBa_K112020
start: 1232
end: 1727
orientation: inline

BBa_K112311
type: SequenceAnnotation
persistent identity: annotation1986751
display id: annotation1986751
version: 1
top level: BBa_K112020
location: https://synbiohub.org/public/igem/BBa_K112020/annotation1986751/range1986751/1
component: BBa_K112311

https://synbiohub.org/public/igem/BBa_K112020/annotation1986751/range1986751/1
type: Range
persistent identity: range1986751
display id: range1986751
version: 1
direction: direction/reverse
top level: BBa_K112020
start: 1734
end: 2071
orientation: inline

BBa_K112999
type: SequenceAnnotation
persistent identity: annotation1986754
display id: annotation1986754
version: 1
top level: BBa_K112020
location: https://synbiohub.org/public/igem/BBa_K112020/annotation1986754/range1986754/1
component: BBa_K112999

https://synbiohub.org/public/igem/BBa_K112020/annotation1986754/range1986754/1
type: Range
persistent identity: range1986754
display id: range1986754
version: 1
direction: direction/reverse
top level: BBa_K112020
start: 2213
end: 2218
orientation: inline

BBa_K112317
type: SequenceAnnotation
persistent identity: annotation1986757
display id: annotation1986757
version: 1
top level: BBa_K112020
location: https://synbiohub.org/public/igem/BBa_K112020/annotation1986757/range1986757/1
component: BBa_K112317

https://synbiohub.org/public/igem/BBa_K112020/annotation1986757/range1986757/1
type: Range
persistent identity: range1986757
display id: range1986757
version: 1
direction: direction/reverse
top level: BBa_K112020
start: 2266
end: 2609
orientation: inline

BBa_K533012
type: ComponentDefinition
persistent identity: BBa K533012
display id: BBa_K533012
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: safety version of pSB1C3 plasmid
creator: ZHANG Yunxiao
created: 2011-10-22T11:00:00Z
modified: 2015-06-15T01:18:23Z
discontinued: true
dominant: false
experience: experience/Works
group u list: _699_
m user id: 4206
owner id: 6884
owning group id: 9
sample status: It's complicated
status: status/Deleted
bookmark: false
mutable description: The plasmid has a suicide gene and a mutation-controller sequence. It can hence eliminate devious mutations and help to restrain biobricks to only our engineered organisms.
mutable notes: Do not use commonly used commercial competent cells when using this plasmid. Special strains are required.
mutable provenance: derived from pSB1C3
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: VF2
sequence annotation: mutated EcoRI
sequence annotation: -10
sequence annotation: -35
sequence annotation: E. coli his operon terminator
sequence annotation: mutated I-SceI site
sequence annotation: Chloramphenicol resistance
sequence annotation: new EcoRI site
sequence annotation: ccdB
sequence annotation: VR
sequence annotation: pMB1
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_K533012_sequence/1

VF2
type: SequenceAnnotation
persistent identity: annotation2161083
display id: annotation2161083
version: 1
top level: BBa_K533012
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K533012/annotation2161083/range2161083/1

https://synbiohub.org/public/igem/BBa_K533012/annotation2161083/range2161083/1
type: Range
persistent identity: range2161083
display id: range2161083
version: 1
direction: direction/forward
top level: BBa_K533012
start: 1933
end: 1952
orientation: inline

mutated EcoRI
type: SequenceAnnotation
persistent identity: annotation2161042
display id: annotation2161042
version: 1
top level: BBa_K533012
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K533012/annotation2161042/range2161042/1

https://synbiohub.org/public/igem/BBa_K533012/annotation2161042/range2161042/1
type: Range
persistent identity: range2161042
display id: range2161042
version: 1
direction: direction/forward
top level: BBa_K533012
start: 2049
end: 2054
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2161043
display id: annotation2161043
version: 1
top level: BBa_K533012
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K533012/annotation2161043/range2161043/1

https://synbiohub.org/public/igem/BBa_K533012/annotation2161043/range2161043/1
type: Range
persistent identity: range2161043
display id: range2161043
version: 1
direction: direction/reverse
top level: BBa_K533012
start: 2527
end: 2532
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2161044
display id: annotation2161044
version: 1
top level: BBa_K533012
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K533012/annotation2161044/range2161044/1

https://synbiohub.org/public/igem/BBa_K533012/annotation2161044/range2161044/1
type: Range
persistent identity: range2161044
display id: range2161044
version: 1
direction: direction/reverse
top level: BBa_K533012
start: 2558
end: 2563
orientation: inline

E. coli his operon terminator
type: SequenceAnnotation
persistent identity: annotation2161048
display id: annotation2161048
version: 1
top level: BBa_K533012
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K533012/annotation2161048/range2161048/1

https://synbiohub.org/public/igem/BBa_K533012/annotation2161048/range2161048/1
type: Range
persistent identity: range2161048
display id: range2161048
version: 1
direction: direction/forward
top level: BBa_K533012
start: 22
end: 93
orientation: inline

mutated I-SceI site
type: SequenceAnnotation
persistent identity: annotation2161049
display id: annotation2161049
version: 1
top level: BBa_K533012
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K533012/annotation2161049/range2161049/1

https://synbiohub.org/public/igem/BBa_K533012/annotation2161049/range2161049/1
type: Range
persistent identity: range2161049
display id: range2161049
version: 1
direction: direction/forward
top level: BBa_K533012
start: 2571
end: 2588
orientation: inline

Chloramphenicol resistance
type: SequenceAnnotation
persistent identity: annotation2161047
display id: annotation2161047
version: 1
top level: BBa_K533012
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K533012/annotation2161047/range2161047/1

https://synbiohub.org/public/igem/BBa_K533012/annotation2161047/range2161047/1
type: Range
persistent identity: range2161047
display id: range2161047
version: 1
direction: direction/reverse
top level: BBa_K533012
start: 1109
end: 1819
orientation: inline

new EcoRI site
type: SequenceAnnotation
persistent identity: annotation2161041
display id: annotation2161041
version: 1
top level: BBa_K533012
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K533012/annotation2161041/range2161041/1

https://synbiohub.org/public/igem/BBa_K533012/annotation2161041/range2161041/1
type: Range
persistent identity: range2161041
display id: range2161041
version: 1
direction: direction/forward
top level: BBa_K533012
start: 2590
end: 2595
orientation: inline

ccdB
type: SequenceAnnotation
persistent identity: annotation2161040
display id: annotation2161040
version: 1
top level: BBa_K533012
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K533012/annotation2161040/range2161040/1

https://synbiohub.org/public/igem/BBa_K533012/annotation2161040/range2161040/1
type: Range
persistent identity: range2161040
display id: range2161040
version: 1
direction: direction/reverse
top level: BBa_K533012
start: 2061
end: 2366
orientation: inline

VR
type: SequenceAnnotation
persistent identity: annotation2161082
display id: annotation2161082
version: 1
top level: BBa_K533012
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K533012/annotation2161082/range2161082/1

https://synbiohub.org/public/igem/BBa_K533012/annotation2161082/range2161082/1
type: Range
persistent identity: range2161082
display id: range2161082
version: 1
direction: direction/reverse
top level: BBa_K533012
start: 157
end: 176
orientation: inline

pMB1
type: SequenceAnnotation
persistent identity: annotation2161046
display id: annotation2161046
version: 1
top level: BBa_K533012
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K533012/annotation2161046/range2161046/1

https://synbiohub.org/public/igem/BBa_K533012/annotation2161046/range2161046/1
type: Range
persistent identity: range2161046
display id: range2161046
version: 1
direction: direction/forward
top level: BBa_K533012
start: 261
end: 874
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation2161045
display id: annotation2161045
version: 1
top level: BBa_K533012
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K533012/annotation2161045/range2161045/1

https://synbiohub.org/public/igem/BBa_K533012/annotation2161045/range2161045/1
type: Range
persistent identity: range2161045
display id: range2161045
version: 1
direction: direction/reverse
top level: BBa_K533012
start: 2022
end: 2041
orientation: inline

BBa_K112304
type: ComponentDefinition
persistent identity: BBa K112304
display id: BBa_K112304
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: {a~lambda lysozyme!} in BBb format
creator: Aron Lau
created: 2008-10-20T11:00:00Z
modified: 2015-05-08T01:09:16Z
discontinued: false
dominant: false
group u list: _224_
m user id: 0
owner id: 2999
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: lambda lysozyme ready to have an rbs attached with a stop codon More information about the BBb Format is available at: [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
mutable notes: PCR al0005/al0002 on pSB3C6-Bjh1501 (512bp, EcoRI/BamHI/DpnI) Sub into pBca1256 (EcoRI/BamHI, 2472+910, L) Product is pBca1256-K112304 ----------------------------------------------- al0005 Forward Biobricking of lambda lysozyme with spacer CCATAGAATTCatgAGATCTgaagatggtagaaatcaataatcaacgtaaggcgttcctcg al0002 Reverse Biobricking of lambda lysozyme with spacer CGttaGGATCCtcatacatcaatctctctgaccgttccgcc
mutable provenance: pSB3C6-Bjh1501
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K112304_sequence/1

BBa_K404113
type: ComponentDefinition
persistent identity: BBa K404113
display id: BBa_K404113
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Mouse guanylate kinase - thymidine kinase TK30 (Fusion protein mGMK_TK30)
creator: Freiburg Bioware 2010
created: 2010-08-27T11:00:00Z
modified: 2015-05-08T01:12:23Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 7049
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: TK30
sequence annotation: mGMK
sequence: https://synbiohub.org/public/igem/BBa_K404113_sequence/1

TK30
type: SequenceAnnotation
persistent identity: annotation2109300
display id: annotation2109300
version: 1
top level: BBa_K404113
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404113/annotation2109300/range2109300/1

https://synbiohub.org/public/igem/BBa_K404113/annotation2109300/range2109300/1
type: Range
persistent identity: range2109300
display id: range2109300
version: 1
direction: direction/forward
top level: BBa_K404113
start: 586
end: 1686
orientation: inline

mGMK
type: SequenceAnnotation
persistent identity: annotation2109299
display id: annotation2109299
version: 1
top level: BBa_K404113
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404113/annotation2109299/range2109299/1

https://synbiohub.org/public/igem/BBa_K404113/annotation2109299/range2109299/1
type: Range
persistent identity: range2109299
display id: range2109299
version: 1
direction: direction/forward
top level: BBa_K404113
start: 1
end: 585
orientation: inline

BBa_K112021
type: ComponentDefinition
persistent identity: BBa K112021
display id: BBa_K112021
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Lambda phage lysis device - no promoter
creator: Bing Xia
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:09:13Z
discontinued: false
dominant: false
group u list: _224_
m user id: 0
owner id: 2998
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This variation uses PconB5 to drive the production of antiholin. ---- This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at: [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_K112317
component: BBa_K112999
component: BBa_K112904
component: BBa_K112305
component: BBa_K112311
component: BBa_K112710
component: BBa_K112903
sequence annotation: BBa_K112999
sequence annotation: BBa_K112904
sequence annotation: BBa_K112903
sequence annotation: BBa_K112305
sequence annotation: BBa_K112311
sequence annotation: BBa_K112710
sequence annotation: BBa_K112317
sequence: https://synbiohub.org/public/igem/BBa_K112021_sequence/1

BBa_K112317
type: Component
persistent identity: component1996415
display id: component1996415
version: 1
top level: BBa_K112021
access: public
definition: BBa_K112317

BBa_K112999
type: Component
persistent identity: component1996410
display id: component1996410
version: 1
top level: BBa_K112021
access: public
definition: https://synbiohub.org/public/igem/BBa_K112999/1

BBa_K112999
type: Component
persistent identity: component1996412
display id: component1996412
version: 1
top level: BBa_K112021
access: public
definition: https://synbiohub.org/public/igem/BBa_K112999/1

BBa_K112999
type: Component
persistent identity: component1996408
display id: component1996408
version: 1
top level: BBa_K112021
access: public
definition: https://synbiohub.org/public/igem/BBa_K112999/1

BBa_K112904
type: Component
persistent identity: component1996413
display id: component1996413
version: 1
top level: BBa_K112021
access: public
definition: https://synbiohub.org/public/igem/BBa_K112904/1

BBa_K112305
type: Component
persistent identity: component1996407
display id: component1996407
version: 1
top level: BBa_K112021
access: public
definition: BBa_K112305

BBa_K112311
type: Component
persistent identity: component1996409
display id: component1996409
version: 1
top level: BBa_K112021
access: public
definition: BBa_K112311

BBa_K112710
type: Component
persistent identity: component1996417
display id: component1996417
version: 1
top level: BBa_K112021
access: public
definition: https://synbiohub.org/public/igem/BBa_K112710/1

BBa_K112999
type: Component
persistent identity: component1996416
display id: component1996416
version: 1
top level: BBa_K112021
access: public
definition: https://synbiohub.org/public/igem/BBa_K112999/1

BBa_K112999
type: Component
persistent identity: component1996414
display id: component1996414
version: 1
top level: BBa_K112021
access: public
definition: https://synbiohub.org/public/igem/BBa_K112999/1

BBa_K112903
type: Component
persistent identity: component1996411
display id: component1996411
version: 1
top level: BBa_K112021
access: public
definition: https://synbiohub.org/public/igem/BBa_K112903/1

BBa_K112999
type: SequenceAnnotation
persistent identity: annotation1996410
display id: annotation1996410
version: 1
top level: BBa_K112021
location: https://synbiohub.org/public/igem/BBa_K112021/annotation1996410/range1996410/1
component: BBa_K112999

https://synbiohub.org/public/igem/BBa_K112021/annotation1996410/range1996410/1
type: Range
persistent identity: range1996410
display id: range1996410
version: 1
direction: direction/reverse
top level: BBa_K112021
start: 841
end: 846
orientation: inline

BBa_K112999
type: SequenceAnnotation
persistent identity: annotation1996416
display id: annotation1996416
version: 1
top level: BBa_K112021
location: https://synbiohub.org/public/igem/BBa_K112021/annotation1996416/range1996416/1
component: BBa_K112999

https://synbiohub.org/public/igem/BBa_K112021/annotation1996416/range1996416/1
type: Range
persistent identity: range1996416
display id: range1996416
version: 1
direction: direction/reverse
top level: BBa_K112021
start: 1379
end: 1384
orientation: inline

BBa_K112999
type: SequenceAnnotation
persistent identity: annotation1996408
display id: annotation1996408
version: 1
top level: BBa_K112021
location: https://synbiohub.org/public/igem/BBa_K112021/annotation1996408/range1996408/1
component: BBa_K112999

https://synbiohub.org/public/igem/BBa_K112021/annotation1996408/range1996408/1
type: Range
persistent identity: range1996408
display id: range1996408
version: 1
direction: direction/reverse
top level: BBa_K112021
start: 497
end: 502
orientation: inline

BBa_K112904
type: SequenceAnnotation
persistent identity: annotation1996413
display id: annotation1996413
version: 1
top level: BBa_K112021
location: https://synbiohub.org/public/igem/BBa_K112021/annotation1996413/range1996413/1
component: BBa_K112904

https://synbiohub.org/public/igem/BBa_K112021/annotation1996413/range1996413/1
type: Range
persistent identity: range1996413
display id: range1996413
version: 1
direction: direction/reverse
top level: BBa_K112021
start: 988
end: 1028
orientation: inline

BBa_K112903
type: SequenceAnnotation
persistent identity: annotation1996411
display id: annotation1996411
version: 1
top level: BBa_K112021
location: https://synbiohub.org/public/igem/BBa_K112021/annotation1996411/range1996411/1
component: BBa_K112903

https://synbiohub.org/public/igem/BBa_K112021/annotation1996411/range1996411/1
type: Range
persistent identity: range1996411
display id: range1996411
version: 1
direction: direction/reverse
top level: BBa_K112021
start: 847
end: 981
orientation: inline

BBa_K112305
type: SequenceAnnotation
persistent identity: annotation1996407
display id: annotation1996407
version: 1
top level: BBa_K112021
location: https://synbiohub.org/public/igem/BBa_K112021/annotation1996407/range1996407/1
component: BBa_K112305

https://synbiohub.org/public/igem/BBa_K112021/annotation1996407/range1996407/1
type: Range
persistent identity: range1996407
display id: range1996407
version: 1
direction: direction/reverse
top level: BBa_K112021
start: 1
end: 496
orientation: inline

BBa_K112311
type: SequenceAnnotation
persistent identity: annotation1996409
display id: annotation1996409
version: 1
top level: BBa_K112021
location: https://synbiohub.org/public/igem/BBa_K112021/annotation1996409/range1996409/1
component: BBa_K112311

https://synbiohub.org/public/igem/BBa_K112021/annotation1996409/range1996409/1
type: Range
persistent identity: range1996409
display id: range1996409
version: 1
direction: direction/reverse
top level: BBa_K112021
start: 503
end: 840
orientation: inline

BBa_K112999
type: SequenceAnnotation
persistent identity: annotation1996412
display id: annotation1996412
version: 1
top level: BBa_K112021
location: https://synbiohub.org/public/igem/BBa_K112021/annotation1996412/range1996412/1
component: BBa_K112999

https://synbiohub.org/public/igem/BBa_K112021/annotation1996412/range1996412/1
type: Range
persistent identity: range1996412
display id: range1996412
version: 1
direction: direction/reverse
top level: BBa_K112021
start: 982
end: 987
orientation: inline

BBa_K112710
type: SequenceAnnotation
persistent identity: annotation1996417
display id: annotation1996417
version: 1
top level: BBa_K112021
location: https://synbiohub.org/public/igem/BBa_K112021/annotation1996417/range1996417/1
component: BBa_K112710

https://synbiohub.org/public/igem/BBa_K112021/annotation1996417/range1996417/1
type: Range
persistent identity: range1996417
display id: range1996417
version: 1
direction: direction/reverse
top level: BBa_K112021
start: 1385
end: 1499
orientation: inline

BBa_K112317
type: SequenceAnnotation
persistent identity: annotation1996415
display id: annotation1996415
version: 1
top level: BBa_K112021
location: https://synbiohub.org/public/igem/BBa_K112021/annotation1996415/range1996415/1
component: BBa_K112317

https://synbiohub.org/public/igem/BBa_K112021/annotation1996415/range1996415/1
type: Range
persistent identity: range1996415
display id: range1996415
version: 1
direction: direction/reverse
top level: BBa_K112021
start: 1035
end: 1378
orientation: inline

BBa_K112999
type: SequenceAnnotation
persistent identity: annotation1996414
display id: annotation1996414
version: 1
top level: BBa_K112021
location: https://synbiohub.org/public/igem/BBa_K112021/annotation1996414/range1996414/1
component: BBa_K112999

https://synbiohub.org/public/igem/BBa_K112021/annotation1996414/range1996414/1
type: Range
persistent identity: range1996414
display id: range1996414
version: 1
direction: direction/reverse
top level: BBa_K112021
start: 1029
end: 1034
orientation: inline

BBa_K112006
type: ComponentDefinition
persistent identity: BBa K112006
display id: BBa_K112006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: T4 antiholin! in BBb
creator: Bing Xia
created: 2008-09-02T11:00:00Z
modified: 2015-05-08T01:09:13Z
discontinued: false
dominant: false
experience: experience/None
group u list: _224_
m user id: 0
owner id: 2998
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: T4 antiholin gene with start and stop codons, in BBb format. ---- This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at: [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
mutable notes: N/A
mutable provenance: PCR bx7/bx8 on Bjh1303 (325 bp, EcoRI/BamHI/DpnI) Sub into pBca1256 (EcoRI/BamHI) Product is pBca1256-K112006 ---------------------------------------- bx7 Forward biobricking of {T4 Antiholin!} ccagtGAATTCatgAGATCTatggccttaaaagcaacagcac bx8 Reverse biobricking of {T4 Antiholin!} cgttaGGATCCtcattcagtctccaatttaatgttc
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K112006_sequence/1

BBa_K112018
type: ComponentDefinition
persistent identity: BBa K112018
display id: BBa_K112018
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Lambda phage lysis device
creator: Bing Xia
created: 2008-10-23T11:00:00Z
modified: 2015-05-08T01:09:13Z
discontinued: false
dominant: false
group u list: _224_
m user id: 0
owner id: 2998
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Lysozyme and holin production are driven by pBad, and antiholin is driven by PconB5.
mutable provenance: Made using 2 antibiotic assembly from basic parts.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_K112305
component: BBa_K112900
component: BBa_K112999
component: BBa_K112904
component: BBa_K112311
component: BBa_K112317
component: BBa_K112903
component: BBa_K112710
sequence annotation: BBa_K112903
sequence annotation: BBa_K112710
sequence annotation: BBa_K112999
sequence annotation: BBa_K112305
sequence annotation: BBa_K112904
sequence annotation: BBa_K112311
sequence annotation: BBa_K112900
sequence annotation: BBa_K112317
sequence: https://synbiohub.org/public/igem/BBa_K112018_sequence/1

BBa_K112305
type: Component
persistent identity: component1986710
display id: component1986710
version: 1
top level: BBa_K112018
access: public
definition: BBa_K112305

BBa_K112900
type: Component
persistent identity: component1986708
display id: component1986708
version: 1
top level: BBa_K112018
access: public
definition: https://synbiohub.org/public/igem/BBa_K112900/1

BBa_K112999
type: Component
persistent identity: component1986717
display id: component1986717
version: 1
top level: BBa_K112018
access: public
definition: https://synbiohub.org/public/igem/BBa_K112999/1

BBa_K112999
type: Component
persistent identity: component1986711
display id: component1986711
version: 1
top level: BBa_K112018
access: public
definition: https://synbiohub.org/public/igem/BBa_K112999/1

BBa_K112999
type: Component
persistent identity: component1986719
display id: component1986719
version: 1
top level: BBa_K112018
access: public
definition: https://synbiohub.org/public/igem/BBa_K112999/1

BBa_K112904
type: Component
persistent identity: component1986716
display id: component1986716
version: 1
top level: BBa_K112018
access: public
definition: https://synbiohub.org/public/igem/BBa_K112904/1

BBa_K112999
type: Component
persistent identity: component1986713
display id: component1986713
version: 1
top level: BBa_K112018
access: public
definition: https://synbiohub.org/public/igem/BBa_K112999/1

BBa_K112311
type: Component
persistent identity: component1986712
display id: component1986712
version: 1
top level: BBa_K112018
access: public
definition: BBa_K112311

BBa_K112317
type: Component
persistent identity: component1986718
display id: component1986718
version: 1
top level: BBa_K112018
access: public
definition: BBa_K112317

BBa_K112903
type: Component
persistent identity: component1986714
display id: component1986714
version: 1
top level: BBa_K112018
access: public
definition: https://synbiohub.org/public/igem/BBa_K112903/1

BBa_K112999
type: Component
persistent identity: component1986709
display id: component1986709
version: 1
top level: BBa_K112018
access: public
definition: https://synbiohub.org/public/igem/BBa_K112999/1

BBa_K112999
type: Component
persistent identity: component1986715
display id: component1986715
version: 1
top level: BBa_K112018
access: public
definition: https://synbiohub.org/public/igem/BBa_K112999/1

BBa_K112710
type: Component
persistent identity: component1986720
display id: component1986720
version: 1
top level: BBa_K112018
access: public
definition: https://synbiohub.org/public/igem/BBa_K112710/1

BBa_K112903
type: SequenceAnnotation
persistent identity: annotation1986714
display id: annotation1986714
version: 1
top level: BBa_K112018
location: https://synbiohub.org/public/igem/BBa_K112018/annotation1986714/range1986714/1
component: BBa_K112903

https://synbiohub.org/public/igem/BBa_K112018/annotation1986714/range1986714/1
type: Range
persistent identity: range1986714
display id: range1986714
version: 1
direction: direction/reverse
top level: BBa_K112018
start: 2078
end: 2212
orientation: inline

BBa_K112710
type: SequenceAnnotation
persistent identity: annotation1986720
display id: annotation1986720
version: 1
top level: BBa_K112018
location: https://synbiohub.org/public/igem/BBa_K112018/annotation1986720/range1986720/1
component: BBa_K112710

https://synbiohub.org/public/igem/BBa_K112018/annotation1986720/range1986720/1
type: Range
persistent identity: range1986720
display id: range1986720
version: 1
direction: direction/reverse
top level: BBa_K112018
start: 2616
end: 2730
orientation: inline

BBa_K112999
type: SequenceAnnotation
persistent identity: annotation1986719
display id: annotation1986719
version: 1
top level: BBa_K112018
location: https://synbiohub.org/public/igem/BBa_K112018/annotation1986719/range1986719/1
component: BBa_K112999

https://synbiohub.org/public/igem/BBa_K112018/annotation1986719/range1986719/1
type: Range
persistent identity: range1986719
display id: range1986719
version: 1
direction: direction/reverse
top level: BBa_K112018
start: 2610
end: 2615
orientation: inline

BBa_K112305
type: SequenceAnnotation
persistent identity: annotation1986710
display id: annotation1986710
version: 1
top level: BBa_K112018
location: https://synbiohub.org/public/igem/BBa_K112018/annotation1986710/range1986710/1
component: BBa_K112305

https://synbiohub.org/public/igem/BBa_K112018/annotation1986710/range1986710/1
type: Range
persistent identity: range1986710
display id: range1986710
version: 1
direction: direction/reverse
top level: BBa_K112018
start: 1232
end: 1727
orientation: inline

BBa_K112999
type: SequenceAnnotation
persistent identity: annotation1986715
display id: annotation1986715
version: 1
top level: BBa_K112018
location: https://synbiohub.org/public/igem/BBa_K112018/annotation1986715/range1986715/1
component: BBa_K112999

https://synbiohub.org/public/igem/BBa_K112018/annotation1986715/range1986715/1
type: Range
persistent identity: range1986715
display id: range1986715
version: 1
direction: direction/reverse
top level: BBa_K112018
start: 2213
end: 2218
orientation: inline

BBa_K112904
type: SequenceAnnotation
persistent identity: annotation1986716
display id: annotation1986716
version: 1
top level: BBa_K112018
location: https://synbiohub.org/public/igem/BBa_K112018/annotation1986716/range1986716/1
component: BBa_K112904

https://synbiohub.org/public/igem/BBa_K112018/annotation1986716/range1986716/1
type: Range
persistent identity: range1986716
display id: range1986716
version: 1
direction: direction/reverse
top level: BBa_K112018
start: 2219
end: 2259
orientation: inline

BBa_K112999
type: SequenceAnnotation
persistent identity: annotation1986709
display id: annotation1986709
version: 1
top level: BBa_K112018
location: https://synbiohub.org/public/igem/BBa_K112018/annotation1986709/range1986709/1
component: BBa_K112999

https://synbiohub.org/public/igem/BBa_K112018/annotation1986709/range1986709/1
type: Range
persistent identity: range1986709
display id: range1986709
version: 1
direction: direction/reverse
top level: BBa_K112018
start: 1226
end: 1231
orientation: inline

BBa_K112311
type: SequenceAnnotation
persistent identity: annotation1986712
display id: annotation1986712
version: 1
top level: BBa_K112018
location: https://synbiohub.org/public/igem/BBa_K112018/annotation1986712/range1986712/1
component: BBa_K112311

https://synbiohub.org/public/igem/BBa_K112018/annotation1986712/range1986712/1
type: Range
persistent identity: range1986712
display id: range1986712
version: 1
direction: direction/reverse
top level: BBa_K112018
start: 1734
end: 2071
orientation: inline

BBa_K112900
type: SequenceAnnotation
persistent identity: annotation1986708
display id: annotation1986708
version: 1
top level: BBa_K112018
location: https://synbiohub.org/public/igem/BBa_K112018/annotation1986708/range1986708/1
component: BBa_K112900

https://synbiohub.org/public/igem/BBa_K112018/annotation1986708/range1986708/1
type: Range
persistent identity: range1986708
display id: range1986708
version: 1
direction: direction/reverse
top level: BBa_K112018
start: 1
end: 1225
orientation: inline

BBa_K112317
type: SequenceAnnotation
persistent identity: annotation1986718
display id: annotation1986718
version: 1
top level: BBa_K112018
location: https://synbiohub.org/public/igem/BBa_K112018/annotation1986718/range1986718/1
component: BBa_K112317

https://synbiohub.org/public/igem/BBa_K112018/annotation1986718/range1986718/1
type: Range
persistent identity: range1986718
display id: range1986718
version: 1
direction: direction/reverse
top level: BBa_K112018
start: 2266
end: 2609
orientation: inline

BBa_K112999
type: SequenceAnnotation
persistent identity: annotation1986711
display id: annotation1986711
version: 1
top level: BBa_K112018
location: https://synbiohub.org/public/igem/BBa_K112018/annotation1986711/range1986711/1
component: BBa_K112999

https://synbiohub.org/public/igem/BBa_K112018/annotation1986711/range1986711/1
type: Range
persistent identity: range1986711
display id: range1986711
version: 1
direction: direction/reverse
top level: BBa_K112018
start: 1728
end: 1733
orientation: inline

BBa_K112999
type: SequenceAnnotation
persistent identity: annotation1986717
display id: annotation1986717
version: 1
top level: BBa_K112018
location: https://synbiohub.org/public/igem/BBa_K112018/annotation1986717/range1986717/1
component: BBa_K112999

https://synbiohub.org/public/igem/BBa_K112018/annotation1986717/range1986717/1
type: Range
persistent identity: range1986717
display id: range1986717
version: 1
direction: direction/reverse
top level: BBa_K112018
start: 2260
end: 2265
orientation: inline

BBa_K112999
type: SequenceAnnotation
persistent identity: annotation1986713
display id: annotation1986713
version: 1
top level: BBa_K112018
location: https://synbiohub.org/public/igem/BBa_K112018/annotation1986713/range1986713/1
component: BBa_K112999

https://synbiohub.org/public/igem/BBa_K112018/annotation1986713/range1986713/1
type: Range
persistent identity: range1986713
display id: range1986713
version: 1
direction: direction/reverse
top level: BBa_K112018
start: 2072
end: 2077
orientation: inline

BBa_K1467203
type: ComponentDefinition
persistent identity: BBa K1467203
display id: BBa_K1467203
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: GoldenGate compatible Bax - cell death inducer
creator: Cara Deal
created: 2014-09-23T11:00:00Z
modified: 2015-05-29T01:35:14Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1846_
m user id: 4206
owner id: 21550
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: BAX coding sequence (Bcl-2-associated X) protein, a pro-apoptotic member of the Bcl-2 protein family which induces cell death. The part has been made compatible with the GoldenGate MoClo Assembly Standard and is free from BsaI and BpiI recognition sequences. Codon-optimised for expression in dicotyledenous plants.
mutable notes: Domestication for GoldenGate by removal of internal BsaI and BpiI recognition sequences. Codon-optimised for expression in dicotyledenous plants.
mutable provenance: Mus musculus
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence: https://synbiohub.org/public/igem/BBa_K1467203_sequence/1

BBa_K112201
type: ComponentDefinition
persistent identity: BBa K112201
display id: BBa_K112201
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: < T4 holin! in BBb
creator: Bing Xia
created: 2008-09-02T11:00:00Z
modified: 2015-05-08T01:09:14Z
discontinued: false
dominant: false
experience: experience/None
group u list: _224_
m user id: 0
owner id: 2998
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: T4 holin gene without a start codon with a stop codon, in BBb format. ---- This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at: [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence: https://synbiohub.org/public/igem/BBa_K112201_sequence/1

BBa_K112312
type: ComponentDefinition
persistent identity: BBa K112312
display id: BBa_K112312
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: {lambda antiholin!}; adheres to Berkeley standard
creator: Aron Lau
created: 2008-10-20T11:00:00Z
modified: 2015-05-08T01:09:16Z
discontinued: false
dominant: false
experience: experience/None
group u list: _224_
m user id: 0
owner id: 2999
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: lambda antiholin with start and stop codons. This part is actually in BBb format. ---- This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at: [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
mutable notes: PCR al0013/al0008 on pSB3C6-Bjh1503 (355bp, EcoRI/BamHI/DpnI) Sub into pBca1256 (EcoRI/BamHI, 2472+910, L) Product is pBca1256-K112312 ----------------------------------------------- al0013 Forward Biobricking of lambda antiholin with start and stop codons CCATAGAATTCatgAGATCTatgaagCtgccagaaaaacatgacc al0008 Reverse Biobricking of lambda antiholin with start and stop codons CGttaGGATCCttattgatttctaccatcttctactccggc
mutable provenance: pSB3C6-Bjh1503
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K112312_sequence/1

BBa_K404112
type: ComponentDefinition
persistent identity: BBa K404112
display id: BBa_K404112
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Cytosine deaminase (CD)
creator: Freiburg Bioware 2010
created: 2010-08-27T11:00:00Z
modified: 2015-05-08T01:12:23Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 7049
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: Cytosine deaminase
sequence: https://synbiohub.org/public/igem/BBa_K404112_sequence/1

Cytosine deaminase
type: SequenceAnnotation
persistent identity: annotation2093175
display id: annotation2093175
version: 1
top level: BBa_K404112
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404112/annotation2093175/range2093175/1

https://synbiohub.org/public/igem/BBa_K404112/annotation2093175/range2093175/1
type: Range
persistent identity: range2093175
display id: range2093175
version: 1
direction: direction/forward
top level: BBa_K404112
start: 1
end: 1281
orientation: inline

BBa_K112307
type: ComponentDefinition
persistent identity: BBa K112307
display id: BBa_K112307
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: {< lambda holin!} in BBb format
creator: Aron Lau
created: 2008-10-20T11:00:00Z
modified: 2015-05-08T01:09:16Z
discontinued: false
dominant: false
experience: experience/None
group u list: _224_
m user id: 0
owner id: 2999
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: lambda holin with stop codon and no start codon in BBb format More information about the BBb Format is available at: [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
mutable notes: PCR al0009/al0008 on pSB3C6-Bjh1502 (346bp, EcoRI/BamHI/DpnI) Sub into pBca1256 (EcoRI/BamHI, 2472+910, L) Product is pBca1256-K112307 ----------------------------------------------- al0009 Forward Biobricking of lambda holin with stop codon and no start codon CCATAGAATTCatgAGATCTccagaaaaacatgacctgttggcc al0008 Reverse Biobricking of lambda holin with stop codon and no start codon CGttaGGATCCttattgatttctaccatcttctactccggc
mutable provenance: pSB3C6-Bjh1502
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence: https://synbiohub.org/public/igem/BBa_K112307_sequence/1

gpS
type: ComponentDefinition
persistent identity: BBa K836004
display id: BBa_K836004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Lysis inhibitor from Enterobacteria phage lambda (codon usage optimized for R. opacus)
creator: Ricardo Alvarado
created: 2012-09-19T11:00:00Z
modified: 2015-05-08T01:13:32Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1095_
m user id: 0
owner id: 11819
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This protein is the one in charge to avoid the formation of pores in the inner membrane of the bacterial cell. It has been optimized for expression in R. opacus. A weak RBS for Gram positive bacteria was used (http://partsregistry.org/Part:BBa_K090506:Design)in order to obtain low concentration of the protein.
mutable notes: We wanted a low concentration of this protein since medium to large concentrations could affect or completely inhibit the lysis device. Basal but constitutive expression of this protein is needed to avoid the formation of pores in the design lysis device.
mutable provenance: The amino sequence was obtained from Uniprot (http://www.uniprot.org/uniprot/P03705#section_seq lysis inhibitor isoform). The codon optimization for R. opacus (avoiding standard restriction sites EcoRI, PstI, XbaI and SpeI) was made using the free software GeneDesigner from DNA 2.0.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
sequence annotation: Lambda antiholin
sequence annotation: BBa_K090506
sequence annotation: Scar
sequence: https://synbiohub.org/public/igem/BBa_K836004_sequence/1

Lambda antiholin
type: SequenceAnnotation
persistent identity: annotation2187517
display id: annotation2187517
version: 1
top level: gpS
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K836004/annotation2187517/range2187517/1

https://synbiohub.org/public/igem/BBa_K836004/annotation2187517/range2187517/1
type: Range
persistent identity: range2187517
display id: range2187517
version: 1
direction: direction/forward
top level: gpS
start: 18
end: 338
orientation: inline

BBa_K090506
type: SequenceAnnotation
persistent identity: annotation2187515
display id: annotation2187515
version: 1
top level: gpS
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K836004/annotation2187515/range2187515/1

https://synbiohub.org/public/igem/BBa_K836004/annotation2187515/range2187515/1
type: Range
persistent identity: range2187515
display id: range2187515
version: 1
direction: direction/forward
top level: gpS
start: 1
end: 11
orientation: inline

Scar
type: SequenceAnnotation
persistent identity: annotation2187516
display id: annotation2187516
version: 1
top level: gpS
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K836004/annotation2187516/range2187516/1

https://synbiohub.org/public/igem/BBa_K836004/annotation2187516/range2187516/1
type: Range
persistent identity: range2187516
display id: range2187516
version: 1
direction: direction/forward
top level: gpS
start: 12
end: 17
orientation: inline

BBa_K1184000
type: ComponentDefinition
persistent identity: BBa K1184000
display id: BBa_K1184000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: KillerRed
creator: Eric Pederson
created: 2013-09-03T11:00:00Z
modified: 2016-01-25T02:40:37Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1497_
m user id: 4206
owner id: 12713
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: KillerRed is a red fluorescent protein that produces reactive oxygen species (ROS) in the presence of yellow-orange light (540-585 nm). KillerRed is engineered from Aequorea victoria GFP (avGFP) to be phototoxic. It has been shown that KillerRed produces superoxide radical anions by reacting with water. Superoxide reacts with the chromophore of KillerRed, causing it to become dark, which ultimately gives rise to a bleaching effect. KillerRed is spectrally similar to mRFP1 with a similar brightness. KillerRed is oligomeric and may form large aggregates. This sequence is codon optimized for mammalian cells and has 14 rare proline codons for E. coli (CCC) and one rare arginine codon (AGA). Codon optimization should be taken into consideration if large amounts of the protein are required. Expression on a high-copy plasmid has produced detectable fluorescent signal, however.
mutable notes: This sequence is not codon optimized for E. coli.
mutable provenance: Engineered from avGFP. Provided by the Bruchez lab at Carnegie Mellon
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Chromophore
sequence annotation: Phototoxic Mutation Ala(163)
sequence annotation: Phototoxic Mutation Asn(147)
sequence annotation: Double Stop
sequence annotation: cds
sequence annotation: Phototoxic Mutation Ser(121)
sequence annotation: Start
sequence annotation: Phototoxic Mutation Glu(70)
sequence: https://synbiohub.org/public/igem/BBa_K1184000_sequence/1

Chromophore
type: SequenceAnnotation
persistent identity: annotation2334958
display id: annotation2334958
version: 1
top level: BBa_K1184000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1184000/annotation2334958/range2334958/1

https://synbiohub.org/public/igem/BBa_K1184000/annotation2334958/range2334958/1
type: Range
persistent identity: range2334958
display id: range2334958
version: 1
direction: direction/forward
top level: BBa_K1184000
start: 199
end: 207
orientation: inline

Phototoxic Mutation Ala(163)
type: SequenceAnnotation
persistent identity: annotation2334965
display id: annotation2334965
version: 1
top level: BBa_K1184000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1184000/annotation2334965/range2334965/1

https://synbiohub.org/public/igem/BBa_K1184000/annotation2334965/range2334965/1
type: Range
persistent identity: range2334965
display id: range2334965
version: 1
direction: direction/forward
top level: BBa_K1184000
start: 487
end: 489
orientation: inline

Phototoxic Mutation Asn(147)
type: SequenceAnnotation
persistent identity: annotation2334960
display id: annotation2334960
version: 1
top level: BBa_K1184000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1184000/annotation2334960/range2334960/1

https://synbiohub.org/public/igem/BBa_K1184000/annotation2334960/range2334960/1
type: Range
persistent identity: range2334960
display id: range2334960
version: 1
direction: direction/forward
top level: BBa_K1184000
start: 439
end: 441
orientation: inline

Double Stop
type: SequenceAnnotation
persistent identity: annotation2334953
display id: annotation2334953
version: 1
top level: BBa_K1184000
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1184000/annotation2334953/range2334953/1

https://synbiohub.org/public/igem/BBa_K1184000/annotation2334953/range2334953/1
type: Range
persistent identity: range2334953
display id: range2334953
version: 1
direction: direction/forward
top level: BBa_K1184000
start: 718
end: 723
orientation: inline

cds
type: SequenceAnnotation
persistent identity: annotation2334955
display id: annotation2334955
version: 1
top level: BBa_K1184000
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1184000/annotation2334955/range2334955/1

https://synbiohub.org/public/igem/BBa_K1184000/annotation2334955/range2334955/1
type: Range
persistent identity: range2334955
display id: range2334955
version: 1
direction: direction/forward
top level: BBa_K1184000
start: 1
end: 717
orientation: inline

Phototoxic Mutation Ser(121)
type: SequenceAnnotation
persistent identity: annotation2334957
display id: annotation2334957
version: 1
top level: BBa_K1184000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1184000/annotation2334957/range2334957/1

https://synbiohub.org/public/igem/BBa_K1184000/annotation2334957/range2334957/1
type: Range
persistent identity: range2334957
display id: range2334957
version: 1
direction: direction/forward
top level: BBa_K1184000
start: 367
end: 369
orientation: inline

Start
type: SequenceAnnotation
persistent identity: annotation2334954
display id: annotation2334954
version: 1
top level: BBa_K1184000
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1184000/annotation2334954/range2334954/1

https://synbiohub.org/public/igem/BBa_K1184000/annotation2334954/range2334954/1
type: Range
persistent identity: range2334954
display id: range2334954
version: 1
direction: direction/forward
top level: BBa_K1184000
start: 1
end: 3
orientation: inline

Phototoxic Mutation Glu(70)
type: SequenceAnnotation
persistent identity: annotation2334956
display id: annotation2334956
version: 1
top level: BBa_K1184000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1184000/annotation2334956/range2334956/1

https://synbiohub.org/public/igem/BBa_K1184000/annotation2334956/range2334956/1
type: Range
persistent identity: range2334956
display id: range2334956
version: 1
direction: direction/forward
top level: BBa_K1184000
start: 208
end: 210
orientation: inline

BBa_K112802
type: ComponentDefinition
persistent identity: BBa K112802
display id: BBa_K112802
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: T4 phage lysis device
creator: Bing Xia
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:09:19Z
discontinued: false
dominant: false
group u list: _224_
m user id: 0
owner id: 2998
owning group id: 9
sample status: No part sequence
status: status/Unavailable
bookmark: false
mutable description: Lysis device based on T4 holin and lysozyme, driven by Pbad. ---- This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at: [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804

BBa_K112019
type: ComponentDefinition
persistent identity: BBa K112019
display id: BBa_K112019
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Lambda phage lysis device
creator: Bing Xia
created: 2008-10-23T11:00:00Z
modified: 2015-05-08T01:09:13Z
discontinued: false
dominant: false
experience: experience/None
group u list: _224_
m user id: 0
owner id: 2998
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Lysozyme and holin production are driven by pBad, and antiholin is driven by PconC5.
mutable provenance: Assembled using 2 antibiotic assembly from basic parts.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_K112317
component: BBa_K112311
component: BBa_K112999
component: BBa_K112900
component: BBa_K112905
component: BBa_K112710
component: BBa_K112903
component: BBa_K112305
sequence annotation: BBa_K112999
sequence annotation: BBa_K112903
sequence annotation: BBa_K112900
sequence annotation: BBa_K112710
sequence annotation: BBa_K112905
sequence annotation: BBa_K112317
sequence annotation: BBa_K112311
sequence annotation: BBa_K112305
sequence: https://synbiohub.org/public/igem/BBa_K112019_sequence/1

BBa_K112317
type: Component
persistent identity: component1986744
display id: component1986744
version: 1
top level: BBa_K112019
access: public
definition: BBa_K112317

BBa_K112311
type: Component
persistent identity: component1986738
display id: component1986738
version: 1
top level: BBa_K112019
access: public
definition: BBa_K112311

BBa_K112999
type: Component
persistent identity: component1986737
display id: component1986737
version: 1
top level: BBa_K112019
access: public
definition: https://synbiohub.org/public/igem/BBa_K112999/1

BBa_K112999
type: Component
persistent identity: component1986743
display id: component1986743
version: 1
top level: BBa_K112019
access: public
definition: https://synbiohub.org/public/igem/BBa_K112999/1

BBa_K112999
type: Component
persistent identity: component1986739
display id: component1986739
version: 1
top level: BBa_K112019
access: public
definition: https://synbiohub.org/public/igem/BBa_K112999/1

BBa_K112999
type: Component
persistent identity: component1986741
display id: component1986741
version: 1
top level: BBa_K112019
access: public
definition: https://synbiohub.org/public/igem/BBa_K112999/1

BBa_K112900
type: Component
persistent identity: component1986734
display id: component1986734
version: 1
top level: BBa_K112019
access: public
definition: https://synbiohub.org/public/igem/BBa_K112900/1

BBa_K112905
type: Component
persistent identity: component1986742
display id: component1986742
version: 1
top level: BBa_K112019
access: public
definition: https://synbiohub.org/public/igem/BBa_K112905/1

BBa_K112999
type: Component
persistent identity: component1986745
display id: component1986745
version: 1
top level: BBa_K112019
access: public
definition: https://synbiohub.org/public/igem/BBa_K112999/1

BBa_K112710
type: Component
persistent identity: component1986746
display id: component1986746
version: 1
top level: BBa_K112019
access: public
definition: https://synbiohub.org/public/igem/BBa_K112710/1

BBa_K112903
type: Component
persistent identity: component1986740
display id: component1986740
version: 1
top level: BBa_K112019
access: public
definition: https://synbiohub.org/public/igem/BBa_K112903/1

BBa_K112305
type: Component
persistent identity: component1986736
display id: component1986736
version: 1
top level: BBa_K112019
access: public
definition: BBa_K112305

BBa_K112999
type: Component
persistent identity: component1986735
display id: component1986735
version: 1
top level: BBa_K112019
access: public
definition: https://synbiohub.org/public/igem/BBa_K112999/1

BBa_K112999
type: SequenceAnnotation
persistent identity: annotation1986743
display id: annotation1986743
version: 1
top level: BBa_K112019
location: https://synbiohub.org/public/igem/BBa_K112019/annotation1986743/range1986743/1
component: BBa_K112999

https://synbiohub.org/public/igem/BBa_K112019/annotation1986743/range1986743/1
type: Range
persistent identity: range1986743
display id: range1986743
version: 1
direction: direction/reverse
top level: BBa_K112019
start: 2260
end: 2265
orientation: inline

BBa_K112999
type: SequenceAnnotation
persistent identity: annotation1986737
display id: annotation1986737
version: 1
top level: BBa_K112019
location: https://synbiohub.org/public/igem/BBa_K112019/annotation1986737/range1986737/1
component: BBa_K112999

https://synbiohub.org/public/igem/BBa_K112019/annotation1986737/range1986737/1
type: Range
persistent identity: range1986737
display id: range1986737
version: 1
direction: direction/reverse
top level: BBa_K112019
start: 1728
end: 1733
orientation: inline

BBa_K112999
type: SequenceAnnotation
persistent identity: annotation1986741
display id: annotation1986741
version: 1
top level: BBa_K112019
location: https://synbiohub.org/public/igem/BBa_K112019/annotation1986741/range1986741/1
component: BBa_K112999

https://synbiohub.org/public/igem/BBa_K112019/annotation1986741/range1986741/1
type: Range
persistent identity: range1986741
display id: range1986741
version: 1
direction: direction/reverse
top level: BBa_K112019
start: 2213
end: 2218
orientation: inline

BBa_K112903
type: SequenceAnnotation
persistent identity: annotation1986740
display id: annotation1986740
version: 1
top level: BBa_K112019
location: https://synbiohub.org/public/igem/BBa_K112019/annotation1986740/range1986740/1
component: BBa_K112903

https://synbiohub.org/public/igem/BBa_K112019/annotation1986740/range1986740/1
type: Range
persistent identity: range1986740
display id: range1986740
version: 1
direction: direction/reverse
top level: BBa_K112019
start: 2078
end: 2212
orientation: inline

BBa_K112900
type: SequenceAnnotation
persistent identity: annotation1986734
display id: annotation1986734
version: 1
top level: BBa_K112019
location: https://synbiohub.org/public/igem/BBa_K112019/annotation1986734/range1986734/1
component: BBa_K112900

https://synbiohub.org/public/igem/BBa_K112019/annotation1986734/range1986734/1
type: Range
persistent identity: range1986734
display id: range1986734
version: 1
direction: direction/reverse
top level: BBa_K112019
start: 1
end: 1225
orientation: inline

BBa_K112999
type: SequenceAnnotation
persistent identity: annotation1986739
display id: annotation1986739
version: 1
top level: BBa_K112019
location: https://synbiohub.org/public/igem/BBa_K112019/annotation1986739/range1986739/1
component: BBa_K112999

https://synbiohub.org/public/igem/BBa_K112019/annotation1986739/range1986739/1
type: Range
persistent identity: range1986739
display id: range1986739
version: 1
direction: direction/reverse
top level: BBa_K112019
start: 2072
end: 2077
orientation: inline

BBa_K112710
type: SequenceAnnotation
persistent identity: annotation1986746
display id: annotation1986746
version: 1
top level: BBa_K112019
location: https://synbiohub.org/public/igem/BBa_K112019/annotation1986746/range1986746/1
component: BBa_K112710

https://synbiohub.org/public/igem/BBa_K112019/annotation1986746/range1986746/1
type: Range
persistent identity: range1986746
display id: range1986746
version: 1
direction: direction/reverse
top level: BBa_K112019
start: 2616
end: 2730
orientation: inline

BBa_K112999
type: SequenceAnnotation
persistent identity: annotation1986745
display id: annotation1986745
version: 1
top level: BBa_K112019
location: https://synbiohub.org/public/igem/BBa_K112019/annotation1986745/range1986745/1
component: BBa_K112999

https://synbiohub.org/public/igem/BBa_K112019/annotation1986745/range1986745/1
type: Range
persistent identity: range1986745
display id: range1986745
version: 1
direction: direction/reverse
top level: BBa_K112019
start: 2610
end: 2615
orientation: inline

BBa_K112905
type: SequenceAnnotation
persistent identity: annotation1986742
display id: annotation1986742
version: 1
top level: BBa_K112019
location: https://synbiohub.org/public/igem/BBa_K112019/annotation1986742/range1986742/1
component: BBa_K112905

https://synbiohub.org/public/igem/BBa_K112019/annotation1986742/range1986742/1
type: Range
persistent identity: range1986742
display id: range1986742
version: 1
direction: direction/reverse
top level: BBa_K112019
start: 2219
end: 2259
orientation: inline

BBa_K112317
type: SequenceAnnotation
persistent identity: annotation1986744
display id: annotation1986744
version: 1
top level: BBa_K112019
location: https://synbiohub.org/public/igem/BBa_K112019/annotation1986744/range1986744/1
component: BBa_K112317

https://synbiohub.org/public/igem/BBa_K112019/annotation1986744/range1986744/1
type: Range
persistent identity: range1986744
display id: range1986744
version: 1
direction: direction/reverse
top level: BBa_K112019
start: 2266
end: 2609
orientation: inline

BBa_K112999
type: SequenceAnnotation
persistent identity: annotation1986735
display id: annotation1986735
version: 1
top level: BBa_K112019
location: https://synbiohub.org/public/igem/BBa_K112019/annotation1986735/range1986735/1
component: BBa_K112999

https://synbiohub.org/public/igem/BBa_K112019/annotation1986735/range1986735/1
type: Range
persistent identity: range1986735
display id: range1986735
version: 1
direction: direction/reverse
top level: BBa_K112019
start: 1226
end: 1231
orientation: inline

BBa_K112311
type: SequenceAnnotation
persistent identity: annotation1986738
display id: annotation1986738
version: 1
top level: BBa_K112019
location: https://synbiohub.org/public/igem/BBa_K112019/annotation1986738/range1986738/1
component: BBa_K112311

https://synbiohub.org/public/igem/BBa_K112019/annotation1986738/range1986738/1
type: Range
persistent identity: range1986738
display id: range1986738
version: 1
direction: direction/reverse
top level: BBa_K112019
start: 1734
end: 2071
orientation: inline

BBa_K112305
type: SequenceAnnotation
persistent identity: annotation1986736
display id: annotation1986736
version: 1
top level: BBa_K112019
location: https://synbiohub.org/public/igem/BBa_K112019/annotation1986736/range1986736/1
component: BBa_K112305

https://synbiohub.org/public/igem/BBa_K112019/annotation1986736/range1986736/1
type: Range
persistent identity: range1986736
display id: range1986736
version: 1
direction: direction/reverse
top level: BBa_K112019
start: 1232
end: 1727
orientation: inline

BBa_K112008
type: ComponentDefinition
persistent identity: BBa K112008
display id: BBa_K112008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: T4 antiholin> in BBb
creator: Bing Xia
created: 2008-09-02T11:00:00Z
modified: 2015-05-08T01:09:13Z
discontinued: false
dominant: false
experience: experience/None
group u list: _224_
m user id: 0
owner id: 2998
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: T4 antiholin gene with a start codon, without a stop codon, in BBb format. ---- This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at: [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
mutable notes: N/A
mutable provenance: PCR bx7/bx10 on Bjh1303 (322 bp, EcoRI/BamHI/DpnI) Sub into pBca1256 (EcoRI/BamHI) Product is pBca1256-K112008 ---------------------------------------- bx7 Forward biobricking of {T4 Antiholin>} ccagtGAATTCatgAGATCTatggccttaaaagcaacagcac bx10 Reverse biobricking of {T4 Antiholin>} cgttaGGATCCttcagtctccaatttaatgttc
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence: https://synbiohub.org/public/igem/BBa_K112008_sequence/1

ccdB
type: ComponentDefinition
persistent identity: BBa P1010
display id: BBa_P1010
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: ccdB cell death gene
creator: Leon Chan
created: 2004-07-27T11:00:00Z
modified: 2015-05-08T01:14:11Z
discontinued: true
dominant: true
experience: experience/Works
group u list: _11_1_
m user id: 0
owner id: 60
owning group id: 7
part status: Deleted
sample status: Discontinued
status: status/Deleted
bookmark: false
mutable description: The ccd operon having a constitutive promoter and the ccdB coding region. ccdB protein is lethal in normal cloning strains. This part is used to aid the process of moving a BioBrick part to a new plasmid. A target plasmid carrying P1010 is cut and mixed with the cut insert. Plasmids that recombine are selected against because they kill the cell that picks them up. P1010 is only provided as plasmid or in DB3.1 which is ccdB resistant.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: T to C mutation
sequence annotation: -35 (rev)
sequence annotation: ccdB (rev)
sequence annotation: ccdA- (rev)
sequence annotation: -10 (rev)
sequence: https://synbiohub.org/public/igem/BBa_P1010_sequence/1

T to C mutation
type: SequenceAnnotation
persistent identity: annotation1747334
display id: annotation1747334
version: 1
top level: ccdB
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_P1010/annotation1747334/range1747334/1

https://synbiohub.org/public/igem/BBa_P1010/annotation1747334/range1747334/1
type: Range
persistent identity: range1747334
display id: range1747334
version: 1
direction: direction/forward
top level: ccdB
start: 467
end: 467
orientation: inline

-35 (rev)
type: SequenceAnnotation
persistent identity: annotation1747336
display id: annotation1747336
version: 1
top level: ccdB
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_P1010/annotation1747336/range1747336/1

https://synbiohub.org/public/igem/BBa_P1010/annotation1747336/range1747336/1
type: Range
persistent identity: range1747336
display id: range1747336
version: 1
direction: direction/reverse
top level: ccdB
start: 618
end: 623
orientation: inline

ccdB (rev)
type: SequenceAnnotation
persistent identity: annotation1747332
display id: annotation1747332
version: 1
top level: ccdB
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_P1010/annotation1747332/range1747332/1

https://synbiohub.org/public/igem/BBa_P1010/annotation1747332/range1747332/1
type: Range
persistent identity: range1747332
display id: range1747332
version: 1
direction: direction/reverse
top level: ccdB
start: 29
end: 334
orientation: inline

ccdA- (rev)
type: SequenceAnnotation
persistent identity: annotation1747333
display id: annotation1747333
version: 1
top level: ccdB
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_P1010/annotation1747333/range1747333/1

https://synbiohub.org/public/igem/BBa_P1010/annotation1747333/range1747333/1
type: Range
persistent identity: range1747333
display id: range1747333
version: 1
direction: direction/reverse
top level: ccdB
start: 336
end: 556
orientation: inline

-10 (rev)
type: SequenceAnnotation
persistent identity: annotation1747335
display id: annotation1747335
version: 1
top level: ccdB
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_P1010/annotation1747335/range1747335/1

https://synbiohub.org/public/igem/BBa_P1010/annotation1747335/range1747335/1
type: Range
persistent identity: range1747335
display id: range1747335
version: 1
direction: direction/reverse
top level: ccdB
start: 595
end: 600
orientation: inline

BBa_K2110008
type: ComponentDefinition
persistent identity: BBa K2110008
display id: BBa_K2110008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: inclusion body induced cell lysis system
creator: Le He
created: 2016-10-05T11:00:00Z
modified: 2016-10-12T09:13:08Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2578_
m user id: 32964
owner id: 32957
owning group id: 9
status: status/Planning
bookmark: true
mutable description: Long Description: This part consists of theCpxR responsive promoter (Part:BBa_K135000), a RBS (Part:BBa_B0034), ddpX gene (Part: BBa_K2110004), two terminators (Part:BBa_B0010, Part:BBa_B0012). The CpxR responsive promoter can response to the inclusion body and misfold protein in the periplasm. When this system coexpress with an exogenous gene like the PETase gene, the forming inclusion body will induce the expression of downstream ddpX gene and cause the cell lysis, which provides much convenience to the purification steps followed.
mutable notes: /
mutable provenance: BBa_K135000 BBa_B0034 BBa_K2110004
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
component: BBa_B0034
component: BBa_K135000
component: BBa_K2110004
sequence annotation: BBa_K135000
sequence annotation: BBa_B0034
sequence annotation: BBa_K2110004
sequence: https://synbiohub.org/public/igem/BBa_K2110008_sequence/1

BBa_B0034
type: Component
persistent identity: component2486553
display id: component2486553
version: 1
top level: BBa_K2110008
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K135000
type: Component
persistent identity: component2486551
display id: component2486551
version: 1
top level: BBa_K2110008
access: public
definition: https://synbiohub.org/public/igem/BBa_K135000/1

BBa_K2110004
type: Component
persistent identity: component2486555
display id: component2486555
version: 1
top level: BBa_K2110008
access: public
definition: BBa_K2110004

BBa_K135000
type: SequenceAnnotation
persistent identity: annotation2486551
display id: annotation2486551
version: 1
top level: BBa_K2110008
location: https://synbiohub.org/public/igem/BBa_K2110008/annotation2486551/range2486551/1
component: BBa_K135000

https://synbiohub.org/public/igem/BBa_K2110008/annotation2486551/range2486551/1
type: Range
persistent identity: range2486551
display id: range2486551
version: 1
direction: direction/reverse
top level: BBa_K2110008
start: 1
end: 55
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2486553
display id: annotation2486553
version: 1
top level: BBa_K2110008
location: https://synbiohub.org/public/igem/BBa_K2110008/annotation2486553/range2486553/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K2110008/annotation2486553/range2486553/1
type: Range
persistent identity: range2486553
display id: range2486553
version: 1
direction: direction/reverse
top level: BBa_K2110008
start: 64
end: 75
orientation: inline

BBa_K2110004
type: SequenceAnnotation
persistent identity: annotation2486555
display id: annotation2486555
version: 1
top level: BBa_K2110008
location: https://synbiohub.org/public/igem/BBa_K2110008/annotation2486555/range2486555/1
component: BBa_K2110004

https://synbiohub.org/public/igem/BBa_K2110008/annotation2486555/range2486555/1
type: Range
persistent identity: range2486555
display id: range2486555
version: 1
direction: direction/reverse
top level: BBa_K2110008
start: 84
end: 665
orientation: inline

BBa_K112317
type: ComponentDefinition
persistent identity: BBa K112317
display id: BBa_K112317
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: {rbs.lambda antiholin!} in BBb format
creator: Aron Lau
created: 2008-10-20T11:00:00Z
modified: 2015-05-08T01:09:16Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _224_
m user id: 0
owner id: 2999
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: lambda antiholin with native rbs site and stop codon. This part is actually in BBb format. ---- This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at: [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
mutable notes: PCR al0016/al0008 on pSB3C6-Bjh1503 (375bp, EcoRI/BamHI/DpnI) Sub into pBca1256 (EcoRI/BamHI, 2472+910, L) Product is pBca1256-K112317 ----------------------------------------------- al0016 Forward Biobricking of lambda antiholin with rbs site CCATAGAATTCatgAGATCTccccttattgggggtaagacatg al0008 Reverse Biobricking of lambda antiholin with rbs site CGttaGGATCCttattgatttctaccatcttctactccggc
mutable provenance: pSB3C6-Bjh1503
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
sequence: https://synbiohub.org/public/igem/BBa_K112317_sequence/1

BBa_K112004
type: ComponentDefinition
persistent identity: BBa K112004
display id: BBa_K112004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: a~T4 holin! in BBb
creator: Bing Xia
created: 2008-09-02T11:00:00Z
modified: 2015-05-08T01:09:13Z
discontinued: false
dominant: false
experience: experience/None
group u list: _224_
m user id: 0
owner id: 2998
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: T4 holin gene ready to have an rbs attached with a stop codon, in BBb format. ---- This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at: [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
mutable notes: N/A
mutable provenance: PCR bx5/bx2 on Bjh1301 (692 bp, EcoRI/BamHI/DpnI) Sub into pBca1256 (EcoRI/BamHI) Product is pBca1256-K112004 ---------------------------------------- bx5 Forward Biobricking of {a~T4 Holin!} ccagtGAATTCatgAGATCTgtctatggcagcacctagaatatc bx2 Reverse Biobricking of {a~T4 Holin!} cgttaGGATCCttatttagcccttcctaatattctggccgcc
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K112004_sequence/1

BBa_K112300
type: ComponentDefinition
persistent identity: BBa K112300
display id: BBa_K112300
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: {lambda lysozyme!} in BBb format
creator: Aron Lau
created: 2008-10-17T11:00:00Z
modified: 2015-05-08T01:09:15Z
discontinued: false
dominant: false
experience: experience/None
group u list: _224_
m user id: 0
owner id: 2999
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: lambda lysozyme with start and stop codons in Bbb format
mutable provenance: pSB3C6-Bjh1501
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K112300_sequence/1

BBa_K2150125
type: ComponentDefinition
persistent identity: BBa K2150125
display id: BBa_K2150125
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Ptet + RBS + RiboJ + toxin 5980 CDS
creator: Zepeng Mu
created: 2016-10-18T11:00:00Z
modified: 2016-10-19T11:25:14Z
discontinued: false
dominant: false
group u list: _2620_
m user id: 29734
owner id: 29734
owning group id: 9
status: status/Planning
bookmark: false
mutable description: a
mutable notes: a
mutable provenance: a
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_C0040
component: BBa_K2150122
component: BBa_B0033
component: BBa_K1679038
component: BBa_R0040
sequence annotation: BBa_K1679038
sequence annotation: BBa_R0040
sequence annotation: BBa_K2150122
sequence annotation: BBa_B0033
sequence annotation: BBa_C0040
sequence: https://synbiohub.org/public/igem/BBa_K2150125_sequence/1

BBa_C0040
type: Component
persistent identity: component2524550
display id: component2524550
version: 1
top level: BBa_K2150125
access: public
definition: https://synbiohub.org/public/igem/BBa_C0040/1

BBa_K2150122
type: Component
persistent identity: component2524561
display id: component2524561
version: 1
top level: BBa_K2150125
access: public
definition: https://synbiohub.org/public/igem/BBa_K2150122/1

BBa_B0033
type: Component
persistent identity: component2524558
display id: component2524558
version: 1
top level: BBa_K2150125
access: public
definition: https://synbiohub.org/public/igem/BBa_B0033/1

BBa_K1679038
type: Component
persistent identity: component2524556
display id: component2524556
version: 1
top level: BBa_K2150125
access: public
definition: https://synbiohub.org/public/igem/BBa_K1679038/1

BBa_R0040
type: Component
persistent identity: component2524551
display id: component2524551
version: 1
top level: BBa_K2150125
access: public
definition: https://synbiohub.org/public/igem/BBa_R0040/1

BBa_K1679038
type: SequenceAnnotation
persistent identity: annotation2524556
display id: annotation2524556
version: 1
top level: BBa_K2150125
location: https://synbiohub.org/public/igem/BBa_K2150125/annotation2524556/range2524556/1
component: BBa_K1679038

https://synbiohub.org/public/igem/BBa_K2150125/annotation2524556/range2524556/1
type: Range
persistent identity: range2524556
display id: range2524556
version: 1
direction: direction/reverse
top level: BBa_K2150125
start: 756
end: 830
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation2524551
display id: annotation2524551
version: 1
top level: BBa_K2150125
location: https://synbiohub.org/public/igem/BBa_K2150125/annotation2524551/range2524551/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_K2150125/annotation2524551/range2524551/1
type: Range
persistent identity: range2524551
display id: range2524551
version: 1
direction: direction/forward
top level: BBa_K2150125
start: 694
end: 747
orientation: inline

BBa_K2150122
type: SequenceAnnotation
persistent identity: annotation2524561
display id: annotation2524561
version: 1
top level: BBa_K2150125
location: https://synbiohub.org/public/igem/BBa_K2150125/annotation2524561/range2524561/1
component: BBa_K2150122

https://synbiohub.org/public/igem/BBa_K2150125/annotation2524561/range2524561/1
type: Range
persistent identity: range2524561
display id: range2524561
version: 1
direction: direction/reverse
top level: BBa_K2150125
start: 856
end: 1221
orientation: inline

BBa_B0033
type: SequenceAnnotation
persistent identity: annotation2524558
display id: annotation2524558
version: 1
top level: BBa_K2150125
location: https://synbiohub.org/public/igem/BBa_K2150125/annotation2524558/range2524558/1
component: BBa_B0033

https://synbiohub.org/public/igem/BBa_K2150125/annotation2524558/range2524558/1
type: Range
persistent identity: range2524558
display id: range2524558
version: 1
direction: direction/forward
top level: BBa_K2150125
start: 839
end: 849
orientation: inline

BBa_C0040
type: SequenceAnnotation
persistent identity: annotation2524550
display id: annotation2524550
version: 1
top level: BBa_K2150125
location: https://synbiohub.org/public/igem/BBa_K2150125/annotation2524550/range2524550/1
component: BBa_C0040

https://synbiohub.org/public/igem/BBa_K2150125/annotation2524550/range2524550/1
type: Range
persistent identity: range2524550
display id: range2524550
version: 1
direction: direction/reverse
top level: BBa_K2150125
start: 1
end: 685
orientation: inline

BBa_K112016
type: ComponentDefinition
persistent identity: BBa K112016
display id: BBa_K112016
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: a~T4 lysozyme! in BBb
creator: Bing Xia
created: 2008-09-02T11:00:00Z
modified: 2015-05-08T01:09:13Z
discontinued: false
dominant: false
group u list: _224_
m user id: 0
owner id: 2998
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: T4 lysozyme gene ready to have an rbs attached in front, with a stop codon, in BBb format. ---- This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at: [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
mutable notes: N/A
mutable provenance: PCR bx17/bx16 on Bjh1302 (530 bp, EcoRI/BamHI/DpnI) Sub into pBca1256 (EcoRI/BamHI) Product is pBca1256-K112016 ---------------------------------------- bx17 Forward biobricking of {a~T4 Endolysin!} ccagtGAATTCatgAGATCTtattatgaatatatttgaaatgttacgtatagatg bx16 Reverse biobricking of {a~T4 Endolysin!} cgttaGGATCCtagatttttatacgcgtcccaagtgcc
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K112016_sequence/1

BBa_K112310
type: ComponentDefinition
persistent identity: BBa K112310
display id: BBa_K112310
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: {a~lambda holin!}; adheres to Berkeley standard
creator: Aron Lau
created: 2008-10-20T11:00:00Z
modified: 2015-05-08T01:09:16Z
discontinued: false
dominant: false
experience: experience/None
group u list: _224_
m user id: 0
owner id: 2999
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: lambda holin ready for rbs addition and has stop codon in BBb format. More information about the BBb Format is available at: [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
mutable notes: PCR al0011/al0008 on pSB3C6-Bjh1502 (353bp, EcoRI/BamHI/DpnI) Sub into pBca1256 (EcoRI/BamHI, 2472+910, L) Product is pBca1256-K112310 ----------------------------------------------- al0011 Forward Biobricking of lambda holin with spacer CCATAGAATTCatgAGATCTgaagatgccagaaaaacatgacctg al0008 Reverse Biobricking of lambda holin with spacer CGttaGGATCCttattgatttctaccatcttctactccggc
mutable provenance: pSB3C6-Bjh1502
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K112310_sequence/1

BBa_K1631018
type: ComponentDefinition
persistent identity: BBa K1631018
display id: BBa_K1631018
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Pconst(Strong) -rbs- CheZ -d.term
creator: Yuto Yamanaka
created: 2015-09-13T11:00:00Z
modified: 2015-09-18T06:21:14Z
discontinued: false
dominant: false
group u list: _2048_
m user id: 23567
owner id: 23567
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Strong, constitutive generator of CheZ.
mutable notes: none
mutable provenance: Assembly
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0015
component: BBa_J23101
component: BBa_K629003
sequence annotation: BBa_B0015
sequence annotation: BBa_J23101
sequence annotation: BBa_K629003
sequence: https://synbiohub.org/public/igem/BBa_K1631018_sequence/1

BBa_B0015
type: Component
persistent identity: component2455610
display id: component2455610
version: 1
top level: BBa_K1631018
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_J23101
type: Component
persistent identity: component2455596
display id: component2455596
version: 1
top level: BBa_K1631018
access: public
definition: https://synbiohub.org/public/igem/BBa_J23101/1

BBa_K629003
type: Component
persistent identity: component2455603
display id: component2455603
version: 1
top level: BBa_K1631018
access: public
definition: https://synbiohub.org/public/igem/BBa_K629003/1

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2455610
display id: annotation2455610
version: 1
top level: BBa_K1631018
location: https://synbiohub.org/public/igem/BBa_K1631018/annotation2455610/range2455610/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K1631018/annotation2455610/range2455610/1
type: Range
persistent identity: range2455610
display id: range2455610
version: 1
direction: direction/reverse
top level: BBa_K1631018
start: 695
end: 823
orientation: inline

BBa_J23101
type: SequenceAnnotation
persistent identity: annotation2455596
display id: annotation2455596
version: 1
top level: BBa_K1631018
location: https://synbiohub.org/public/igem/BBa_K1631018/annotation2455596/range2455596/1
component: BBa_J23101

https://synbiohub.org/public/igem/BBa_K1631018/annotation2455596/range2455596/1
type: Range
persistent identity: range2455596
display id: range2455596
version: 1
direction: direction/reverse
top level: BBa_K1631018
start: 1
end: 35
orientation: inline

BBa_K629003
type: SequenceAnnotation
persistent identity: annotation2455603
display id: annotation2455603
version: 1
top level: BBa_K1631018
location: https://synbiohub.org/public/igem/BBa_K1631018/annotation2455603/range2455603/1
component: BBa_K629003

https://synbiohub.org/public/igem/BBa_K1631018/annotation2455603/range2455603/1
type: Range
persistent identity: range2455603
display id: range2455603
version: 1
direction: direction/reverse
top level: BBa_K1631018
start: 42
end: 686
orientation: inline

BBa_K404315
type: ComponentDefinition
persistent identity: BBa K404315
display id: BBa_K404315
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Mouse guanylate kinase - tymidine kinase SR39 (Fusion protein mGMK_SR39)
creator: Freiburg Bioware 2010
created: 2010-10-11T11:00:00Z
modified: 2015-05-08T01:12:25Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 6733
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: mGMK
sequence annotation: sr39
sequence: https://synbiohub.org/public/igem/BBa_K404315_sequence/1

mGMK
type: SequenceAnnotation
persistent identity: annotation2094854
display id: annotation2094854
version: 1
top level: BBa_K404315
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404315/annotation2094854/range2094854/1

https://synbiohub.org/public/igem/BBa_K404315/annotation2094854/range2094854/1
type: Range
persistent identity: range2094854
display id: range2094854
version: 1
direction: direction/forward
top level: BBa_K404315
start: 1
end: 585
orientation: inline

sr39
type: SequenceAnnotation
persistent identity: annotation2094840
display id: annotation2094840
version: 1
top level: BBa_K404315
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404315/annotation2094840/range2094840/1

https://synbiohub.org/public/igem/BBa_K404315/annotation2094840/range2094840/1
type: Range
persistent identity: range2094840
display id: range2094840
version: 1
direction: direction/forward
top level: BBa_K404315
start: 586
end: 1686
orientation: inline

BBa_K112009
type: ComponentDefinition
persistent identity: BBa K112009
display id: BBa_K112009
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: <T4 antiholin> in BBb
creator: Bing Xia
created: 2008-09-02T11:00:00Z
modified: 2015-05-08T01:09:13Z
discontinued: false
dominant: false
experience: experience/None
group u list: _224_
m user id: 0
owner id: 2998
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: T4 antiholin gene without start and stop codons, in BBb format. ---- This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at: [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
mutable notes: N/A
mutable provenance: PCR bx9/bx10 on Bjh1303 (319 bp, EcoRI/BamHI/DpnI) Sub into pBca1256 (EcoRI/BamHI) Product is pBca1256-K112009 ---------------------------------------- bx9 Forward biobricking of {<T4 Antiholin>} ccagtGAATTCatgAGATCTgccttaaaagcaacagcac bx10 Reverse biobricking of {<T4 Antiholin>} cgttaGGATCCttcagtctccaatttaatgttc
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K112009_sequence/1

toxin ParE
type: ComponentDefinition
persistent identity: BBa K2150104
display id: BBa_K2150104
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: 6249 toxin CDS
creator: Zepeng Mu
created: 2016-10-11T11:00:00Z
modified: 2016-10-13T11:15:01Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2620_
m user id: 29734
owner id: 29734
owning group id: 9
status: status/Planning
bookmark: false
mutable description: a
mutable notes: a
mutable provenance: a
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: ParD2
sequence annotation: misc
sequence: https://synbiohub.org/public/igem/BBa_K2150104_sequence/1

ParD2
type: SequenceAnnotation
persistent identity: annotation2499113
display id: annotation2499113
version: 1
top level: toxin ParE
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K2150104/annotation2499113/range2499113/1

https://synbiohub.org/public/igem/BBa_K2150104/annotation2499113/range2499113/1
type: Range
persistent identity: range2499113
display id: range2499113
version: 1
direction: direction/forward
top level: toxin ParE
start: 1
end: 316
orientation: inline

misc
type: SequenceAnnotation
persistent identity: annotation2499115
display id: annotation2499115
version: 1
top level: toxin ParE
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K2150104/annotation2499115/range2499115/1

https://synbiohub.org/public/igem/BBa_K2150104/annotation2499115/range2499115/1
type: Range
persistent identity: range2499115
display id: range2499115
version: 1
direction: direction/forward
top level: toxin ParE
start: 317
end: 319
orientation: inline

BBa_K864150
type: ComponentDefinition
persistent identity: BBa K864150
display id: BBa_K864150
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Cat-SacB cassette for scarless chromosomal knockout
creator: Erik Lundin
created: 2012-09-24T11:00:00Z
modified: 2015-05-08T01:13:37Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1124_
m user id: 0
owner id: 9827
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Cat-SacB Consists of chloramphenicol resistance marker BBa_P1004 and the Bacillus subtilis levansucrase SacB BBa_K322921
mutable notes: -
mutable provenance: Partsregistry
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_P1004
component: BBa_K322921
sequence annotation: BBa_P1004
sequence annotation: BBa_K322921
sequence: https://synbiohub.org/public/igem/BBa_K864150_sequence/1

BBa_P1004
type: Component
persistent identity: component2197679
display id: component2197679
version: 1
top level: BBa_K864150
access: public
definition: https://synbiohub.org/public/igem/BBa_P1004/1

BBa_K322921
type: Component
persistent identity: component2197683
display id: component2197683
version: 1
top level: BBa_K864150
access: public
definition: https://synbiohub.org/public/igem/BBa_K322921/1

BBa_P1004
type: SequenceAnnotation
persistent identity: annotation2197679
display id: annotation2197679
version: 1
top level: BBa_K864150
location: https://synbiohub.org/public/igem/BBa_K864150/annotation2197679/range2197679/1
component: BBa_P1004

https://synbiohub.org/public/igem/BBa_K864150/annotation2197679/range2197679/1
type: Range
persistent identity: range2197679
display id: range2197679
version: 1
direction: direction/reverse
top level: BBa_K864150
start: 1
end: 769
orientation: inline

BBa_K322921
type: SequenceAnnotation
persistent identity: annotation2197683
display id: annotation2197683
version: 1
top level: BBa_K864150
location: https://synbiohub.org/public/igem/BBa_K864150/annotation2197683/range2197683/1
component: BBa_K322921

https://synbiohub.org/public/igem/BBa_K864150/annotation2197683/range2197683/1
type: Range
persistent identity: range2197683
display id: range2197683
version: 1
direction: direction/reverse
top level: BBa_K864150
start: 778
end: 2214
orientation: inline

BBa_K1631022
type: ComponentDefinition
persistent identity: BBa K1631022
display id: BBa_K1631022
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Plux -rbs- Barnase -rbs- GFP -d.term
creator: Yuto Yamanaka
created: 2015-09-13T11:00:00Z
modified: 2015-09-18T06:26:39Z
discontinued: false
dominant: false
group u list: _2048_
m user id: 23567
owner id: 23567
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Barnase and GFP is co-expressed by luxR/AHL complex.
mutable notes: none
mutable provenance: Assembly
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_R0062
component: BBa_K1631001
sequence annotation: BBa_K1631001
sequence annotation: BBa_R0062
sequence: https://synbiohub.org/public/igem/BBa_K1631022_sequence/1

BBa_R0062
type: Component
persistent identity: component2455723
display id: component2455723
version: 1
top level: BBa_K1631022
access: public
definition: https://synbiohub.org/public/igem/BBa_R0062/1

BBa_K1631001
type: Component
persistent identity: component2455743
display id: component2455743
version: 1
top level: BBa_K1631022
access: public
definition: https://synbiohub.org/public/igem/BBa_K1631001/1

BBa_K1631001
type: SequenceAnnotation
persistent identity: annotation2455743
display id: annotation2455743
version: 1
top level: BBa_K1631022
location: https://synbiohub.org/public/igem/BBa_K1631022/annotation2455743/range2455743/1
component: BBa_K1631001

https://synbiohub.org/public/igem/BBa_K1631022/annotation2455743/range2455743/1
type: Range
persistent identity: range2455743
display id: range2455743
version: 1
direction: direction/reverse
top level: BBa_K1631022
start: 64
end: 1330
orientation: inline

BBa_R0062
type: SequenceAnnotation
persistent identity: annotation2455723
display id: annotation2455723
version: 1
top level: BBa_K1631022
location: https://synbiohub.org/public/igem/BBa_K1631022/annotation2455723/range2455723/1
component: BBa_R0062

https://synbiohub.org/public/igem/BBa_K1631022/annotation2455723/range2455723/1
type: Range
persistent identity: range2455723
display id: range2455723
version: 1
direction: direction/forward
top level: BBa_K1631022
start: 1
end: 55
orientation: inline

BBa_K2040122
type: ComponentDefinition
persistent identity: BBa K2040122
display id: BBa_K2040122
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: KillerRed + NLS
creator: Chilam Poon
created: 2016-10-13T11:00:00Z
modified: 2016-10-19T01:35:29Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2507_
m user id: 30360
owner id: 30360
owning group id: 9
status: status/Planning
bookmark: false
mutable description: A SV40 nuclear localization signal was added to the phototoxic protein KillerRed.
mutable notes: NLS
mutable provenance: BBa_K1184000
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: KillerRed
sequence annotation: SV40 NLS
sequence: https://synbiohub.org/public/igem/BBa_K2040122_sequence/1

KillerRed
type: SequenceAnnotation
persistent identity: annotation2518117
display id: annotation2518117
version: 1
top level: BBa_K2040122
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K2040122/annotation2518117/range2518117/1

https://synbiohub.org/public/igem/BBa_K2040122/annotation2518117/range2518117/1
type: Range
persistent identity: range2518117
display id: range2518117
version: 1
direction: direction/forward
top level: BBa_K2040122
start: 1
end: 717
orientation: inline

SV40 NLS
type: SequenceAnnotation
persistent identity: annotation2502105
display id: annotation2502105
version: 1
top level: BBa_K2040122
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K2040122/annotation2502105/range2502105/1

https://synbiohub.org/public/igem/BBa_K2040122/annotation2502105/range2502105/1
type: Range
persistent identity: range2502105
display id: range2502105
version: 1
direction: direction/forward
top level: BBa_K2040122
start: 718
end: 741
orientation: inline

BBa_K112013
type: ComponentDefinition
persistent identity: BBa K112013
display id: BBa_K112013
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: < T4 lysozyme! in BBb
creator: Bing Xia
created: 2008-09-02T11:00:00Z
modified: 2015-05-08T01:09:13Z
discontinued: false
dominant: false
experience: experience/None
group u list: _224_
m user id: 0
owner id: 2998
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: T4 lysozyme gene without a start codon, with a stop codon, in BBb format. ---- This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at: [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
mutable notes: N/A
mutable provenance: PCR bx15/bx14 on Bjh1302 (523 bp, EcoRI/BamHI/DpnI) Sub into pBca1256 (EcoRI/BamHI) Product is pBca1256-K112013 ---------------------------------------- bx15 Forward biobricking of {<T4 Endolysin!} ccagtGAATTCatgAGATCTaatatatttgaaatgttacgtatagatg bx14 Reverse biobricking of {<T4 Endolysin!} cgttaGGATCCttatagatttttatacgcgtcccaagtgcc
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence: https://synbiohub.org/public/igem/BBa_K112013_sequence/1

BBa_K112022
type: ComponentDefinition
persistent identity: BBa K112022
display id: BBa_K112022
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Lambda phage lysis device - no promoter
creator: Bing Xia
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:09:13Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _224_
m user id: 0
owner id: 2998
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This variation uses PconC5 to drive the production of antiholin. ---- This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at: [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_K112311
component: BBa_K112905
component: BBa_K112903
component: BBa_K112999
component: BBa_K112710
component: BBa_K112305
component: BBa_K112317
sequence annotation: BBa_K112305
sequence annotation: BBa_K112905
sequence annotation: BBa_K112999
sequence annotation: BBa_K112317
sequence annotation: BBa_K112311
sequence annotation: BBa_K112710
sequence annotation: BBa_K112903
sequence: https://synbiohub.org/public/igem/BBa_K112022_sequence/1

BBa_K112311
type: Component
persistent identity: component1996532
display id: component1996532
version: 1
top level: BBa_K112022
access: public
definition: BBa_K112311

BBa_K112905
type: Component
persistent identity: component1996536
display id: component1996536
version: 1
top level: BBa_K112022
access: public
definition: https://synbiohub.org/public/igem/BBa_K112905/1

BBa_K112903
type: Component
persistent identity: component1996534
display id: component1996534
version: 1
top level: BBa_K112022
access: public
definition: https://synbiohub.org/public/igem/BBa_K112903/1

BBa_K112999
type: Component
persistent identity: component1996537
display id: component1996537
version: 1
top level: BBa_K112022
access: public
definition: https://synbiohub.org/public/igem/BBa_K112999/1

BBa_K112710
type: Component
persistent identity: component1996540
display id: component1996540
version: 1
top level: BBa_K112022
access: public
definition: https://synbiohub.org/public/igem/BBa_K112710/1

BBa_K112999
type: Component
persistent identity: component1996535
display id: component1996535
version: 1
top level: BBa_K112022
access: public
definition: https://synbiohub.org/public/igem/BBa_K112999/1

BBa_K112305
type: Component
persistent identity: component1996530
display id: component1996530
version: 1
top level: BBa_K112022
access: public
definition: BBa_K112305

BBa_K112317
type: Component
persistent identity: component1996538
display id: component1996538
version: 1
top level: BBa_K112022
access: public
definition: BBa_K112317

BBa_K112999
type: Component
persistent identity: component1996533
display id: component1996533
version: 1
top level: BBa_K112022
access: public
definition: https://synbiohub.org/public/igem/BBa_K112999/1

BBa_K112999
type: Component
persistent identity: component1996539
display id: component1996539
version: 1
top level: BBa_K112022
access: public
definition: https://synbiohub.org/public/igem/BBa_K112999/1

BBa_K112999
type: Component
persistent identity: component1996531
display id: component1996531
version: 1
top level: BBa_K112022
access: public
definition: https://synbiohub.org/public/igem/BBa_K112999/1

BBa_K112305
type: SequenceAnnotation
persistent identity: annotation1996530
display id: annotation1996530
version: 1
top level: BBa_K112022
location: https://synbiohub.org/public/igem/BBa_K112022/annotation1996530/range1996530/1
component: BBa_K112305

https://synbiohub.org/public/igem/BBa_K112022/annotation1996530/range1996530/1
type: Range
persistent identity: range1996530
display id: range1996530
version: 1
direction: direction/reverse
top level: BBa_K112022
start: 1
end: 496
orientation: inline

BBa_K112905
type: SequenceAnnotation
persistent identity: annotation1996536
display id: annotation1996536
version: 1
top level: BBa_K112022
location: https://synbiohub.org/public/igem/BBa_K112022/annotation1996536/range1996536/1
component: BBa_K112905

https://synbiohub.org/public/igem/BBa_K112022/annotation1996536/range1996536/1
type: Range
persistent identity: range1996536
display id: range1996536
version: 1
direction: direction/reverse
top level: BBa_K112022
start: 988
end: 1028
orientation: inline

BBa_K112999
type: SequenceAnnotation
persistent identity: annotation1996531
display id: annotation1996531
version: 1
top level: BBa_K112022
location: https://synbiohub.org/public/igem/BBa_K112022/annotation1996531/range1996531/1
component: BBa_K112999

https://synbiohub.org/public/igem/BBa_K112022/annotation1996531/range1996531/1
type: Range
persistent identity: range1996531
display id: range1996531
version: 1
direction: direction/reverse
top level: BBa_K112022
start: 497
end: 502
orientation: inline

BBa_K112999
type: SequenceAnnotation
persistent identity: annotation1996533
display id: annotation1996533
version: 1
top level: BBa_K112022
location: https://synbiohub.org/public/igem/BBa_K112022/annotation1996533/range1996533/1
component: BBa_K112999

https://synbiohub.org/public/igem/BBa_K112022/annotation1996533/range1996533/1
type: Range
persistent identity: range1996533
display id: range1996533
version: 1
direction: direction/reverse
top level: BBa_K112022
start: 841
end: 846
orientation: inline

BBa_K112999
type: SequenceAnnotation
persistent identity: annotation1996535
display id: annotation1996535
version: 1
top level: BBa_K112022
location: https://synbiohub.org/public/igem/BBa_K112022/annotation1996535/range1996535/1
component: BBa_K112999

https://synbiohub.org/public/igem/BBa_K112022/annotation1996535/range1996535/1
type: Range
persistent identity: range1996535
display id: range1996535
version: 1
direction: direction/reverse
top level: BBa_K112022
start: 982
end: 987
orientation: inline

BBa_K112317
type: SequenceAnnotation
persistent identity: annotation1996538
display id: annotation1996538
version: 1
top level: BBa_K112022
location: https://synbiohub.org/public/igem/BBa_K112022/annotation1996538/range1996538/1
component: BBa_K112317

https://synbiohub.org/public/igem/BBa_K112022/annotation1996538/range1996538/1
type: Range
persistent identity: range1996538
display id: range1996538
version: 1
direction: direction/reverse
top level: BBa_K112022
start: 1035
end: 1378
orientation: inline

BBa_K112999
type: SequenceAnnotation
persistent identity: annotation1996537
display id: annotation1996537
version: 1
top level: BBa_K112022
location: https://synbiohub.org/public/igem/BBa_K112022/annotation1996537/range1996537/1
component: BBa_K112999

https://synbiohub.org/public/igem/BBa_K112022/annotation1996537/range1996537/1
type: Range
persistent identity: range1996537
display id: range1996537
version: 1
direction: direction/reverse
top level: BBa_K112022
start: 1029
end: 1034
orientation: inline

BBa_K112311
type: SequenceAnnotation
persistent identity: annotation1996532
display id: annotation1996532
version: 1
top level: BBa_K112022
location: https://synbiohub.org/public/igem/BBa_K112022/annotation1996532/range1996532/1
component: BBa_K112311

https://synbiohub.org/public/igem/BBa_K112022/annotation1996532/range1996532/1
type: Range
persistent identity: range1996532
display id: range1996532
version: 1
direction: direction/reverse
top level: BBa_K112022
start: 503
end: 840
orientation: inline

BBa_K112999
type: SequenceAnnotation
persistent identity: annotation1996539
display id: annotation1996539
version: 1
top level: BBa_K112022
location: https://synbiohub.org/public/igem/BBa_K112022/annotation1996539/range1996539/1
component: BBa_K112999

https://synbiohub.org/public/igem/BBa_K112022/annotation1996539/range1996539/1
type: Range
persistent identity: range1996539
display id: range1996539
version: 1
direction: direction/reverse
top level: BBa_K112022
start: 1379
end: 1384
orientation: inline

BBa_K112710
type: SequenceAnnotation
persistent identity: annotation1996540
display id: annotation1996540
version: 1
top level: BBa_K112022
location: https://synbiohub.org/public/igem/BBa_K112022/annotation1996540/range1996540/1
component: BBa_K112710

https://synbiohub.org/public/igem/BBa_K112022/annotation1996540/range1996540/1
type: Range
persistent identity: range1996540
display id: range1996540
version: 1
direction: direction/reverse
top level: BBa_K112022
start: 1385
end: 1499
orientation: inline

BBa_K112903
type: SequenceAnnotation
persistent identity: annotation1996534
display id: annotation1996534
version: 1
top level: BBa_K112022
location: https://synbiohub.org/public/igem/BBa_K112022/annotation1996534/range1996534/1
component: BBa_K112903

https://synbiohub.org/public/igem/BBa_K112022/annotation1996534/range1996534/1
type: Range
persistent identity: range1996534
display id: range1996534
version: 1
direction: direction/reverse
top level: BBa_K112022
start: 847
end: 981
orientation: inline

BBa_K137131
type: ComponentDefinition
persistent identity: BBa K137131
display id: BBa_K137131
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Lactose inducible lysis cassette expression
creator: Josh Michener
created: 2008-10-22T11:00:00Z
modified: 2015-05-08T01:10:11Z
discontinued: false
dominant: false
experience: experience/None
group u list: _187_
m user id: 0
owner id: 253
owning group id: 6
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Temp
mutable notes: Constitutive LacI expression ensures tight repression of lysis.
mutable provenance: Lysis cassette is cloned from Lambda Zap II genomic DNA.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_S04114
component: BBa_S04106
sequence annotation: BBa_S04106
sequence annotation: BBa_S04114
sequence: https://synbiohub.org/public/igem/BBa_K137131_sequence/1

BBa_S04114
type: Component
persistent identity: component2276506
display id: component2276506
version: 1
top level: BBa_K137131
access: public
definition: https://synbiohub.org/public/igem/BBa_S04114/1

BBa_S04106
type: Component
persistent identity: component2276497
display id: component2276497
version: 1
top level: BBa_K137131
access: public
definition: https://synbiohub.org/public/igem/BBa_S04106/1

BBa_S04106
type: SequenceAnnotation
persistent identity: annotation2276497
display id: annotation2276497
version: 1
top level: BBa_K137131
location: https://synbiohub.org/public/igem/BBa_K137131/annotation2276497/range2276497/1
component: BBa_S04106

https://synbiohub.org/public/igem/BBa_K137131/annotation2276497/range2276497/1
type: Range
persistent identity: range2276497
display id: range2276497
version: 1
direction: direction/reverse
top level: BBa_K137131
start: 1
end: 1462
orientation: inline

BBa_S04114
type: SequenceAnnotation
persistent identity: annotation2276506
display id: annotation2276506
version: 1
top level: BBa_K137131
location: https://synbiohub.org/public/igem/BBa_K137131/annotation2276506/range2276506/1
component: BBa_S04114

https://synbiohub.org/public/igem/BBa_K137131/annotation2276506/range2276506/1
type: Range
persistent identity: range2276506
display id: range2276506
version: 1
direction: direction/reverse
top level: BBa_K137131
start: 1471
end: 2923
orientation: inline

BBa_K1631012
type: ComponentDefinition
persistent identity: BBa K1631012
display id: BBa_K1631012
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Plac -rbs- Barnase -rbs- GFP -d.term
creator: Yuto Yamanaka
created: 2015-09-13T11:00:00Z
modified: 2015-09-18T06:17:31Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2048_
m user id: 23567
owner id: 23567
owning group id: 9
status: status/Planning
bookmark: true
mutable description: Barnase and GFP is co-expressed by IPTG induction.
mutable notes: none
mutable provenance: Assembly
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0015
component: BBa_K082003
component: BBa_B0034
component: BBa_R0011
component: BBa_K1631000
sequence annotation: BBa_K1631000
sequence annotation: BBa_B0015
sequence annotation: BBa_R0011
sequence annotation: BBa_K082003
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K1631012_sequence/1

BBa_B0015
type: Component
persistent identity: component2473194
display id: component2473194
version: 1
top level: BBa_K1631012
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_K082003
type: Component
persistent identity: component2473187
display id: component2473187
version: 1
top level: BBa_K1631012
access: public
definition: https://synbiohub.org/public/igem/BBa_K082003/1

BBa_B0034
type: Component
persistent identity: component2473184
display id: component2473184
version: 1
top level: BBa_K1631012
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_R0011
type: Component
persistent identity: component2473174
display id: component2473174
version: 1
top level: BBa_K1631012
access: public
definition: https://synbiohub.org/public/igem/BBa_R0011/1

BBa_K1631000
type: Component
persistent identity: component2473182
display id: component2473182
version: 1
top level: BBa_K1631012
access: public
definition: https://synbiohub.org/public/igem/BBa_K1631000/1

BBa_K1631000
type: SequenceAnnotation
persistent identity: annotation2473182
display id: annotation2473182
version: 1
top level: BBa_K1631012
location: https://synbiohub.org/public/igem/BBa_K1631012/annotation2473182/range2473182/1
component: BBa_K1631000

https://synbiohub.org/public/igem/BBa_K1631012/annotation2473182/range2473182/1
type: Range
persistent identity: range2473182
display id: range2473182
version: 1
direction: direction/reverse
top level: BBa_K1631012
start: 64
end: 411
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2473194
display id: annotation2473194
version: 1
top level: BBa_K1631012
location: https://synbiohub.org/public/igem/BBa_K1631012/annotation2473194/range2473194/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K1631012/annotation2473194/range2473194/1
type: Range
persistent identity: range2473194
display id: range2473194
version: 1
direction: direction/reverse
top level: BBa_K1631012
start: 1202
end: 1330
orientation: inline

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation2473174
display id: annotation2473174
version: 1
top level: BBa_K1631012
location: https://synbiohub.org/public/igem/BBa_K1631012/annotation2473174/range2473174/1
component: BBa_R0011

https://synbiohub.org/public/igem/BBa_K1631012/annotation2473174/range2473174/1
type: Range
persistent identity: range2473174
display id: range2473174
version: 1
direction: direction/forward
top level: BBa_K1631012
start: 1
end: 54
orientation: inline

BBa_K082003
type: SequenceAnnotation
persistent identity: annotation2473187
display id: annotation2473187
version: 1
top level: BBa_K1631012
location: https://synbiohub.org/public/igem/BBa_K1631012/annotation2473187/range2473187/1
component: BBa_K082003

https://synbiohub.org/public/igem/BBa_K1631012/annotation2473187/range2473187/1
type: Range
persistent identity: range2473187
display id: range2473187
version: 1
direction: direction/reverse
top level: BBa_K1631012
start: 438
end: 1193
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2473184
display id: annotation2473184
version: 1
top level: BBa_K1631012
location: https://synbiohub.org/public/igem/BBa_K1631012/annotation2473184/range2473184/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1631012/annotation2473184/range2473184/1
type: Range
persistent identity: range2473184
display id: range2473184
version: 1
direction: direction/reverse
top level: BBa_K1631012
start: 420
end: 431
orientation: inline

BBa_K108001
type: ComponentDefinition
persistent identity: BBa K108001
display id: BBa_K108001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: SRRz
creator: Qi Liu
created: 2008-10-14T11:00:00Z
modified: 2015-05-08T01:09:03Z
discontinued: false
dominant: false
experience: experience/None
group u list: _222_
m user id: 0
owner id: 2671
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: SRRz
mutable notes: ...
mutable provenance: Alcaligenes eutrophus H16
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Rz
sequence annotation: S
sequence annotation: R
sequence: https://synbiohub.org/public/igem/BBa_K108001_sequence/1

Rz
type: SequenceAnnotation
persistent identity: annotation1986692
display id: annotation1986692
version: 1
top level: BBa_K108001
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K108001/annotation1986692/range1986692/1

https://synbiohub.org/public/igem/BBa_K108001/annotation1986692/range1986692/1
type: Range
persistent identity: range1986692
display id: range1986692
version: 1
direction: direction/forward
top level: BBa_K108001
start: 781
end: 1239
orientation: inline

S
type: SequenceAnnotation
persistent identity: annotation1986662
display id: annotation1986662
version: 1
top level: BBa_K108001
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K108001/annotation1986662/range1986662/1

https://synbiohub.org/public/igem/BBa_K108001/annotation1986662/range1986662/1
type: Range
persistent identity: range1986662
display id: range1986662
version: 1
direction: direction/forward
top level: BBa_K108001
start: 1
end: 321
orientation: inline

R
type: SequenceAnnotation
persistent identity: annotation1986663
display id: annotation1986663
version: 1
top level: BBa_K108001
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K108001/annotation1986663/range1986663/1

https://synbiohub.org/public/igem/BBa_K108001/annotation1986663/range1986663/1
type: Range
persistent identity: range1986663
display id: range1986663
version: 1
direction: direction/forward
top level: BBa_K108001
start: 308
end: 781
orientation: inline

BBa_K112313
type: ComponentDefinition
persistent identity: BBa K112313
display id: BBa_K112313
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: {< lambda antiholin!}; adheres to Berkeley standard
creator: Aron Lau
created: 2008-10-20T11:00:00Z
modified: 2015-05-08T01:09:16Z
discontinued: false
dominant: false
experience: experience/None
group u list: _224_
m user id: 0
owner id: 2999
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: lambda antiholin with stop codon and no start codon. This part is actually in BBb format. ---- This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at: [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
mutable notes: PCR al0014/al0008 on pSB3C6-Bjh1503 (352bp, EcoRI/BamHI/DpnI) Sub into pBca1256 (EcoRI/BamHI, 2472+910, L) Product is pBca1256-K112313 ----------------------------------------------- al0014 Forward Biobricking of lambda antiholin with stop codon and no start codon CCATAGAATTCatgAGATCTaagCtgccagaaaaacatgacctg al0008 Reverse Biobricking of lambda antiholin with stop codon and no start codon CGttaGGATCCttattgatttctaccatcttctactccggc
mutable provenance: pSB3C6-Bjh1503
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence: https://synbiohub.org/public/igem/BBa_K112313_sequence/1

BBa_K112316
type: ComponentDefinition
persistent identity: BBa K112316
display id: BBa_K112316
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: {a~lambda antiholin!}; adheres to Berkeley standard
creator: Aron Lau
created: 2008-10-20T11:00:00Z
modified: 2015-05-08T01:09:16Z
discontinued: false
dominant: false
experience: experience/None
group u list: _224_
m user id: 0
owner id: 2999
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: lambda antiholin ready for rbs addition and has stop codon. This part is actually in BBb format. ---- This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at: [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
mutable notes: PCR al0015/al0008 on pSB3C6-Bjh1503 (359bp, EcoRI/BamHI/DpnI) Sub into pBca1256 (EcoRI/BamHI, 2472+910, L) Product is pBca1256-K112316 ----------------------------------------------- al0015 Forward Biobricking of lambda antiholin with spacer CCATAGAATTCatgAGATCTagacatgaagCtgccagaaaaacatgacc al0008 Reverse Biobricking of lambda antiholin with spacer CGttaGGATCCttattgatttctaccatcttctactccggc
mutable provenance: pSB3C6-Bjh1503
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K112316_sequence/1

CcdB
type: ComponentDefinition
persistent identity: BBa K1318000
display id: BBa_K1318000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CcdB toxin
creator: Laurence Van Melderen
created: 2014-10-01T11:00:00Z
modified: 2015-07-09T11:28:18Z
discontinued: true
dominant: false
experience: experience/Works
group u list: _1693_
m user id: 4206
owner id: 20061
owning group id: 9
sample status: In stock
status: status/Deleted
bookmark: true
mutable description: CcdB is a toxin that poisons and kills E. coli by inhibiting the DNA gyrase and inducing double strand breaks. CcdB activity can be inhibited by the CcdA antitoxin. This gene is under the control of a galactose inducible promoter
mutable notes: CcdB is toxic so it has to be expressed in conjunction with CcdA or not on a galactose-containing medium.
mutable provenance: E. coli fertility factor (F plasmid)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: CcdB start
sequence annotation: CcdB stop
sequence annotation: CcdB
sequence: https://synbiohub.org/public/igem/BBa_K1318000_sequence/1

CcdB start
type: SequenceAnnotation
persistent identity: annotation2416917
display id: annotation2416917
version: 1
top level: CcdB
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1318000/annotation2416917/range2416917/1

https://synbiohub.org/public/igem/BBa_K1318000/annotation2416917/range2416917/1
type: Range
persistent identity: range2416917
display id: range2416917
version: 1
direction: direction/forward
top level: CcdB
start: 1
end: 3
orientation: inline

CcdB stop
type: SequenceAnnotation
persistent identity: annotation2416918
display id: annotation2416918
version: 1
top level: CcdB
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1318000/annotation2416918/range2416918/1

https://synbiohub.org/public/igem/BBa_K1318000/annotation2416918/range2416918/1
type: Range
persistent identity: range2416918
display id: range2416918
version: 1
direction: direction/forward
top level: CcdB
start: 307
end: 309
orientation: inline

CcdB
type: SequenceAnnotation
persistent identity: annotation2416919
display id: annotation2416919
version: 1
top level: CcdB
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1318000/annotation2416919/range2416919/1

https://synbiohub.org/public/igem/BBa_K1318000/annotation2416919/range2416919/1
type: Range
persistent identity: range2416919
display id: range2416919
version: 1
direction: direction/forward
top level: CcdB
start: 1
end: 309
orientation: inline

BBa_K112007
type: ComponentDefinition
persistent identity: BBa K112007
display id: BBa_K112007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: < T4 antiholin! in BBb
creator: Bing Xia
created: 2008-09-02T11:00:00Z
modified: 2015-05-08T01:09:13Z
discontinued: false
dominant: false
experience: experience/None
group u list: _224_
m user id: 0
owner id: 2998
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: T4 antiholin gene with a start codon, with a stop codon, in BBb format. ---- This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at: [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
mutable notes: N/A
mutable provenance: PCR bx9/bx8 on Bjh1303 (322 bp, EcoRI/BamHI/DpnI) Sub into pBca1256 (EcoRI/BamHI) Product is pBca1256-K112007 ---------------------------------------- bx9 Forward biobricking of {<T4 Antiholin!} ccagtGAATTCatgAGATCTgccttaaaagcaacagcac bx8 Reverse biobricking of {<T4 Antiholin!} cgttaGGATCCtcattcagtctccaatttaatgttc
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence: https://synbiohub.org/public/igem/BBa_K112007_sequence/1

BBa_K112807
type: ComponentDefinition
persistent identity: BBa K112807
display id: BBa_K112807
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: [T4 antiholin]
creator: Jin Huh
created: 2008-10-31T12:00:00Z
modified: 2015-05-08T01:09:19Z
discontinued: false
dominant: true
experience: experience/None
group u list: _224_
m user id: 0
owner id: 3025
owning group id: 171
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Antiholin binds holin and prevents holin multimers from forming pores on the inner membrane of bacteria.
mutable notes: Higher constitutive expression of antiholin compared to the expression of holin is required for the stable lysis device.
mutable provenance: Enterobacteria phage T4 http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?val=29366675&from=59202&to=59495&view=gbwithparts
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: T4 antiholin
sequence: https://synbiohub.org/public/igem/BBa_K112807_sequence/1

T4 antiholin
type: SequenceAnnotation
persistent identity: annotation1999019
display id: annotation1999019
version: 1
top level: BBa_K112807
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K112807/annotation1999019/range1999019/1

https://synbiohub.org/public/igem/BBa_K112807/annotation1999019/range1999019/1
type: Range
persistent identity: range1999019
display id: range1999019
version: 1
direction: direction/forward
top level: BBa_K112807
start: 15
end: 308
orientation: inline

BBa_K112314
type: ComponentDefinition
persistent identity: BBa K112314
display id: BBa_K112314
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: { lambda antiholin >}; adheres to Berkeley standard
creator: Aron Lau
created: 2008-10-20T11:00:00Z
modified: 2015-05-08T01:09:16Z
discontinued: false
dominant: false
experience: experience/None
group u list: _224_
m user id: 0
owner id: 2999
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: lambda antiholin with start codon and no stop codon. This part is actually in BBb format. ---- This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at: [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
mutable notes: PCR al0013/al0010 on pSB3C6-Bjh1503 (352bp, EcoRI/BamHI/DpnI) Sub into pBca1256 (EcoRI/BamHI, 2472+910, L) Product is pBca1256-K112314 ----------------------------------------------- al0013 Forward Biobricking of lambda antiholin with start codon and no stop codon CCATAGAATTCatgAGATCTatgaagCtgccagaaaaacatgacc al0010 Reverse Biobricking of lambda antiholin with start codon and no stop codon CGttaGGATCCttgatttctaccatcttctactccggc
mutable provenance: pSB3C6-Bjh1503
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence: https://synbiohub.org/public/igem/BBa_K112314_sequence/1

BBa_K112306
type: ComponentDefinition
persistent identity: BBa K112306
display id: BBa_K112306
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: {lambda holin!} in BBb format
creator: Aron Lau
created: 2008-10-20T11:00:00Z
modified: 2015-05-08T01:09:16Z
discontinued: false
dominant: true
experience: experience/None
group u list: _224_
m user id: 0
owner id: 2999
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: lambda holin with start and stop codons in BBb format. More information about the BBb Format is available at: [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
mutable notes: PCR al0007/al0008 on pSB3C6-Bjh1502 (349bp, EcoRI/BamHI/DpnI) Sub into pBca1256 (EcoRI/BamHI, 2472+910, L) Product is pBca1256-K112306 ----------------------------------------------- al0007 Forward Biobricking of lambda holin with start and stop codons CCATAGAATTCatgAGATCTatgccagaaaaacatgacctgttgg al0008 Reverse Biobricking of lambda holin with start and stop codons CGttaGGATCCttattgatttctaccatcttctactccggc
mutable provenance: pSB3C6-Bjh1502
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K112306_sequence/1

BBa_K404111
type: ComponentDefinition
persistent identity: BBa K404111
display id: BBa_K404111
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Mouse guanylate kinase (mGMK)
creator: Freiburg Bioware 2010
created: 2010-08-27T11:00:00Z
modified: 2015-05-08T01:12:23Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 7049
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: mouse guanylate kinase
sequence: https://synbiohub.org/public/igem/BBa_K404111_sequence/1

mouse guanylate kinase
type: SequenceAnnotation
persistent identity: annotation2093174
display id: annotation2093174
version: 1
top level: BBa_K404111
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404111/annotation2093174/range2093174/1

https://synbiohub.org/public/igem/BBa_K404111/annotation2093174/range2093174/1
type: Range
persistent identity: range2093174
display id: range2093174
version: 1
direction: direction/forward
top level: BBa_K404111
start: 1
end: 585
orientation: inline

BBa_K112005
type: ComponentDefinition
persistent identity: BBa K112005
display id: BBa_K112005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: rbs.T4 holin!
creator: Bing Xia
created: 2008-09-02T11:00:00Z
modified: 2015-05-08T01:09:13Z
discontinued: false
dominant: false
experience: experience/None
group u list: _224_
m user id: 0
owner id: 2998
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: T4 holin gene with a native rbs and a stop codon, in BBb format. ---- This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at: [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
mutable notes: N/A
mutable provenance: PCR bx6/bx2 on Bjh1301 (704 bp, EcoRI/BamHI/DpnI) Sub into pBca1256 (EcoRI/BamHI) Product is pBca1256-K112005 ---------------------------------------- bx6 Forward Biobricking of {rbs.T4 Holin!} ccagtGAATTCatgAGATCTcttaaaaggagggtctatggcagcacctagaatatc bx2 Reverse Biobricking of {rbs.T4 Holin!} cgttaGGATCCttatttagcccttcctaatattctggccgcc
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
sequence: https://synbiohub.org/public/igem/BBa_K112005_sequence/1

BBa_K2110004
type: ComponentDefinition
persistent identity: BBa K2110004
display id: BBa_K2110004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: D-alanyl-D-alanine dipeptidase gene
creator: Le He
created: 2016-10-04T11:00:00Z
modified: 2016-10-05T11:21:26Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2578_
m user id: 32957
owner id: 32957
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Peptidoglycan is the major constituent of cell wall of bacterial like E.coli. The D-alanyl-D-alanine dipeptidase gene (ddpX) exists in the genome of E.coli. The counterpart of ddpX, VanX, which has the same effect as ddpX, exists in many gram-positive bacterial like Enterococcus faecalis and Streptomyces toyocaensis because they need it to hydrolyze the D-Ala-D-Ala and transfer the D-Ala-D-Ala residue of peptidoglycan to D-Ala-D-Lac residue so that the antibiotic vancomycin cannot combine the peptidoglycan to cause the destruction of cell wall. However, in gram-negative bacterial like E.coli, which own the robust outer membrane that can resist the vancomycin, the hydrolase ddpX with the same effects also exists. This is strange because the gram-negative bacterial have no necessity to own this kind of seemly dangerous hydrolase. Actually the ddpX in E.coli has another vital use when they are under starvation conditions. The ddpX can hydrolyze the D-Ala-D-Ala in their cell wall to produce the D-Ala as the carbon source to maintain their life. This mechanism is only carried out when they are under starvation conditions. If the ddpX gene is overexpressed, the cell wall will be damaged and cell lysis will occur.
mutable notes: Enhanced promoter is required
mutable provenance: It comes from the genome of E.coli
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: BioBrick
sequence: https://synbiohub.org/public/igem/BBa_K2110004_sequence/1

BioBrick
type: SequenceAnnotation
persistent identity: annotation2486167
display id: annotation2486167
version: 1
top level: BBa_K2110004
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K2110004/annotation2486167/range2486167/1

https://synbiohub.org/public/igem/BBa_K2110004/annotation2486167/range2486167/1
type: Range
persistent identity: range2486167
display id: range2486167
version: 1
direction: direction/forward
top level: BBa_K2110004
start: 1
end: 582
orientation: inline

BBa_K748006
type: ComponentDefinition
persistent identity: BBa K748006
display id: BBa_K748006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: S.aureus killing device. Truncated lysostaphin coding sequence with IPTG inducible promoter.
creator: Lei Qiao
created: 2012-09-14T11:00:00Z
modified: 2015-05-08T01:13:12Z
discontinued: false
dominant: false
group u list: _999_
m user id: 0
owner id: 8564
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Lysostaphin is a zinc metalloenzyme that has a specific lytic action against S.aureus. Lysostaphin has activities of three enzymes namely, endo-β-N-acetyl glucosamidase, N-acteyl-muramyl-L-alanine amidase, glycylglycine endopeptidase. Glycylglycine endopeptidase lyses staphylococcal cells by hydrolyzing glycylglycine bonds in the poly-glycine bridges which form cross links between glycopeptide chains in cell wall peptidoglycan of S.aureus cells. The wild-type lysostaphin gene encodes a preproenzyme, and the conversion of prolysostaphin to mature lysostaphin occurs extracellularly and involves the removal of the hydrophilic tandem repeat portion of the proenzyme. In order to directly produce mature lysostaphin, we truncate the preprolysostaphin and prolysostaphin sequence. With the inducing of IPTG, the device can erradicate S.aureus through the production and release of lysostaphin.
mutable notes: In order to directly produce mature lysostaphin, we truncate the preprolysostaphin and prolysostaphin sequence.
mutable provenance: de novo synthesis
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K748002
component: BBa_B0034
component: BBa_B0015
component: BBa_R0011
sequence annotation: BBa_B0034
sequence annotation: BBa_B0015
sequence annotation: BBa_R0011
sequence annotation: BBa_K748002
sequence: https://synbiohub.org/public/igem/BBa_K748006_sequence/1

BBa_K748002
type: Component
persistent identity: component2183387
display id: component2183387
version: 1
top level: BBa_K748006
access: public
definition: https://synbiohub.org/public/igem/BBa_K748002/1

BBa_B0034
type: Component
persistent identity: component2183386
display id: component2183386
version: 1
top level: BBa_K748006
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0015
type: Component
persistent identity: component2183394
display id: component2183394
version: 1
top level: BBa_K748006
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_R0011
type: Component
persistent identity: component2183380
display id: component2183380
version: 1
top level: BBa_K748006
access: public
definition: https://synbiohub.org/public/igem/BBa_R0011/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2183386
display id: annotation2183386
version: 1
top level: BBa_K748006
location: https://synbiohub.org/public/igem/BBa_K748006/annotation2183386/range2183386/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K748006/annotation2183386/range2183386/1
type: Range
persistent identity: range2183386
display id: range2183386
version: 1
direction: direction/reverse
top level: BBa_K748006
start: 64
end: 75
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2183394
display id: annotation2183394
version: 1
top level: BBa_K748006
location: https://synbiohub.org/public/igem/BBa_K748006/annotation2183394/range2183394/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K748006/annotation2183394/range2183394/1
type: Range
persistent identity: range2183394
display id: range2183394
version: 1
direction: direction/reverse
top level: BBa_K748006
start: 831
end: 959
orientation: inline

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation2183380
display id: annotation2183380
version: 1
top level: BBa_K748006
location: https://synbiohub.org/public/igem/BBa_K748006/annotation2183380/range2183380/1
component: BBa_R0011

https://synbiohub.org/public/igem/BBa_K748006/annotation2183380/range2183380/1
type: Range
persistent identity: range2183380
display id: range2183380
version: 1
direction: direction/forward
top level: BBa_K748006
start: 1
end: 54
orientation: inline

BBa_K748002
type: SequenceAnnotation
persistent identity: annotation2183387
display id: annotation2183387
version: 1
top level: BBa_K748006
location: https://synbiohub.org/public/igem/BBa_K748006/annotation2183387/range2183387/1
component: BBa_K748002

https://synbiohub.org/public/igem/BBa_K748006/annotation2183387/range2183387/1
type: Range
persistent identity: range2183387
display id: range2183387
version: 1
direction: direction/reverse
top level: BBa_K748006
start: 82
end: 822
orientation: inline

BBa_K112311
type: ComponentDefinition
persistent identity: BBa K112311
display id: BBa_K112311
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: {rbs.lambda holin!} in BBb format
creator: Aron Lau
created: 2008-10-20T11:00:00Z
modified: 2015-05-08T01:09:16Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _224_
m user id: 0
owner id: 2999
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: lambda holin with rbs site and stop codon in BBb format. More information about the BBb Format is available at: [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
mutable notes: PCR al0012/al0008 on pSB3C6-Bjh1502 (369bp, EcoRI/BamHI/DpnI) Sub into pBca1256 (EcoRI/BamHI, 2472+910, L) Product is pBca1256-K112311 ----------------------------------------------- al0012 Forward Biobricking of lambda holin with rbs site CCATAGAATTCatgAGATCTattgggggtaagacCtgaag al0008 Reverse Biobricking of lambda holin with rbs site CGttaGGATCCttattgatttctaccatcttctactccggc
mutable provenance: pSB3C6-Bjh1502
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
sequence: https://synbiohub.org/public/igem/BBa_K112311_sequence/1

BBa_K112301
type: ComponentDefinition
persistent identity: BBa K112301
display id: BBa_K112301
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: {< lambda lysozyme!} in BBb format
creator: Aron Lau
created: 2008-10-20T11:00:00Z
modified: 2015-05-08T01:09:16Z
discontinued: false
dominant: false
experience: experience/None
group u list: _224_
m user id: 0
owner id: 2999
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: Lambda Lysozyme with stop codon and no start codon in BBb format
mutable provenance: PCR al0003/al0002 on pSB3C6-Bjh1501 (505bp, EcoRI/BamHI/DpnI) Sub into pBca1256 (EcoRI/BamHI, 2472+910, L) Product is pBca1256-K112301 ----------------------------------------------- al0003 Forward Biobricking of Lambda Lysozyme with stop codon and no start codon CCATAGAATTCatgAGATCTgtagaaatcaataatcaacgtaaggcgttcctcg al0002 Reverse Biobricking of Lambda Lysozyme with stop codon and no start codon CGttaGGATCCtcatacatcaatctctctgaccgttccgcc
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence: https://synbiohub.org/public/igem/BBa_K112301_sequence/1

Lysis
type: ComponentDefinition
persistent identity: BBa K137126
display id: BBa_K137126
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Lambda Lysis Cassette
creator: Robert Ovadia
created: 2008-10-15T11:00:00Z
modified: 2015-05-08T01:10:11Z
discontinued: false
dominant: false
experience: experience/None
group u list: _187_
m user id: 0
owner id: 2018
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Lambda Lysis Cassette genes required for cell lysis. Contains holin/anti-holin, endolysin, and rz / rz1 genes.
mutable notes: None
mutable provenance: Lambda Genomic DNA
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence: https://synbiohub.org/public/igem/BBa_K137126_sequence/1

BBa_K404110
type: ComponentDefinition
persistent identity: BBa K404110
display id: BBa_K404110
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Thyminidine kinase (mutant SR39)
creator: Freiburg Bioware 2010
created: 2010-08-27T11:00:00Z
modified: 2015-05-08T01:12:23Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 7049
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: TK30
sequence annotation: SR39
sequence: https://synbiohub.org/public/igem/BBa_K404110_sequence/1

TK30
type: SequenceAnnotation
persistent identity: annotation2097519
display id: annotation2097519
version: 1
top level: BBa_K404110
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404110/annotation2097519/range2097519/1

https://synbiohub.org/public/igem/BBa_K404110/annotation2097519/range2097519/1
type: Range
persistent identity: range2097519
display id: range2097519
version: 1
direction: direction/forward
top level: BBa_K404110
start: 1
end: 265
orientation: inline

TK30
type: SequenceAnnotation
persistent identity: annotation2094943
display id: annotation2094943
version: 1
top level: BBa_K404110
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404110/annotation2094943/range2094943/1

https://synbiohub.org/public/igem/BBa_K404110/annotation2094943/range2094943/1
type: Range
persistent identity: range2094943
display id: range2094943
version: 1
direction: direction/forward
top level: BBa_K404110
start: 501
end: 1020
orientation: inline

SR39
type: SequenceAnnotation
persistent identity: annotation2097520
display id: annotation2097520
version: 1
top level: BBa_K404110
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404110/annotation2097520/range2097520/1

https://synbiohub.org/public/igem/BBa_K404110/annotation2097520/range2097520/1
type: Range
persistent identity: range2097520
display id: range2097520
version: 1
direction: direction/forward
top level: BBa_K404110
start: 266
end: 500
orientation: inline

BBa_K112011
type: ComponentDefinition
persistent identity: BBa K112011
display id: BBa_K112011
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: rbs.T4 antiholin! in BBb
creator: Bing Xia
created: 2008-09-02T11:00:00Z
modified: 2015-05-08T01:09:13Z
discontinued: false
dominant: false
experience: experience/None
group u list: _224_
m user id: 0
owner id: 2998
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: T4 antiholin gene with a native rbs, with a stop codon, in BBb format. ---- This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at: [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
mutable notes: N/A
mutable provenance: PCR bx12/bx8 on Bjh1303 (339 bp, EcoRI/BamHI/DpnI) Sub into pBca1256 (EcoRI/BamHI) Product is pBca1256-K112011 ---------------------------------------- bx12 Forward biobricking of {rbs.T4 Antiholin!} ccagtGAATTCatgAGATCTgataggaggcctttatggccttaaaagcaacagc bx8 Reverse biobricking of {rbs.T4 Antiholin!} cgttaGGATCCtcattcagtctccaatttaatgttc
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
sequence: https://synbiohub.org/public/igem/BBa_K112011_sequence/1

BBa_K112002
type: ComponentDefinition
persistent identity: BBa K112002
display id: BBa_K112002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: T4 holin, without stop codon, berkeley standard
creator: Bing Xia
created: 2008-09-02T11:00:00Z
modified: 2015-05-08T01:09:13Z
discontinued: false
dominant: false
experience: experience/None
group u list: _224_
m user id: 0
owner id: 2998
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: T4 holin gene with a start codon, without a stop codon, in BBb format. ---- This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at: [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K112002_sequence/1

BBa_K1631025
type: ComponentDefinition
persistent identity: BBa K1631025
display id: BBa_K1631025
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Colicin-E3 cassette (Medium)
creator: Yuto Yamanaka
created: 2015-09-13T11:00:00Z
modified: 2015-09-18T09:38:28Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2048_
m user id: 23567
owner id: 23567
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Colicin-E3, its immunity protein, and colisin lysis protein is constitutively expressed.(under BBa_J23110) When this device works, Colicin-E3 is released to media, and kill E. coli strains which does not have immunity protein.
mutable notes: none
mutable provenance: Assembly
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_K1631009
component: BBa_J23110
sequence annotation: BBa_K1631009
sequence annotation: BBa_J23110
sequence: https://synbiohub.org/public/igem/BBa_K1631025_sequence/1

BBa_K1631009
type: Component
persistent identity: component2455825
display id: component2455825
version: 1
top level: BBa_K1631025
access: public
definition: https://synbiohub.org/public/igem/BBa_K1631009/1

BBa_J23110
type: Component
persistent identity: component2455808
display id: component2455808
version: 1
top level: BBa_K1631025
access: public
definition: https://synbiohub.org/public/igem/BBa_J23110/1

BBa_K1631009
type: SequenceAnnotation
persistent identity: annotation2455825
display id: annotation2455825
version: 1
top level: BBa_K1631025
location: https://synbiohub.org/public/igem/BBa_K1631025/annotation2455825/range2455825/1
component: BBa_K1631009

https://synbiohub.org/public/igem/BBa_K1631025/annotation2455825/range2455825/1
type: Range
persistent identity: range2455825
display id: range2455825
version: 1
direction: direction/reverse
top level: BBa_K1631025
start: 44
end: 2290
orientation: inline

BBa_J23110
type: SequenceAnnotation
persistent identity: annotation2455808
display id: annotation2455808
version: 1
top level: BBa_K1631025
location: https://synbiohub.org/public/igem/BBa_K1631025/annotation2455808/range2455808/1
component: BBa_J23110

https://synbiohub.org/public/igem/BBa_K1631025/annotation2455808/range2455808/1
type: Range
persistent identity: range2455808
display id: range2455808
version: 1
direction: direction/reverse
top level: BBa_K1631025
start: 1
end: 35
orientation: inline

BBa_K112305
type: ComponentDefinition
persistent identity: BBa K112305
display id: BBa_K112305
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: {rbs.lambda lysozyme!} in BBb format
creator: Aron Lau
created: 2008-10-20T11:00:00Z
modified: 2015-05-08T01:09:16Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _224_
m user id: 0
owner id: 2999
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: lambda lysozyme with native rbs site in BBb format. More information about the BBb Format is available at: [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
mutable notes: PCR al0006/al0002 on pSB3C6-Bjh1501 (527bp, EcoRI/BamHI/DpnI) Sub into pBca1256 (EcoRI/BamHI, 2472+910, L) Product is pBca1256-K112305 ----------------------------------------------- al0006 Forward Biobricking of lambda lysozyme with rbs site CCATAGAATTCatgAGATCTaaaaaagccggagtagaagatgg al0002 Reverse Biobricking of lambda lysozyme with rbs site CGttaGGATCCtcatacatcaatctctctgaccgttccgcc
mutable provenance: pSB3C6-Bjh1501
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
sequence: https://synbiohub.org/public/igem/BBa_K112305_sequence/1

BBa_K1054001
type: ComponentDefinition
persistent identity: BBa K1054001
display id: BBa_K1054001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: protein E of Phage phiX174 under pBAD
creator: Jiasheng Wang
created: 2013-09-09T11:00:00Z
modified: 2015-05-08T01:08:56Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1361_
m user id: 0
owner id: 15789
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: Prontein E is a membrane protein that inhibits MraY, an enzyme required for murein synthesis, and promotes cell lysis in a manner analogous to cell wall synthesis inhibitors like penicillin. However, it will keep the periplasmic space of Gram-negative strain intact. In this part, protein E will be expressed when L-arabinose is available.
mutable notes: No
mutable provenance: Enterobacteria phage phiX174
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_B0015
component: BBa_K1054000
component: BBa_J61107
component: BBa_K206000
sequence annotation: BBa_B0015
sequence annotation: BBa_K1054000
sequence annotation: BBa_K206000
sequence annotation: BBa_J61107
sequence: https://synbiohub.org/public/igem/BBa_K1054001_sequence/1

BBa_B0015
type: Component
persistent identity: component2338507
display id: component2338507
version: 1
top level: BBa_K1054001
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_K1054000
type: Component
persistent identity: component2338500
display id: component2338500
version: 1
top level: BBa_K1054001
access: public
definition: https://synbiohub.org/public/igem/BBa_K1054000/1

BBa_J61107
type: Component
persistent identity: component2338497
display id: component2338497
version: 1
top level: BBa_K1054001
access: public
definition: https://synbiohub.org/public/igem/BBa_J61107/1

BBa_K206000
type: Component
persistent identity: component2338496
display id: component2338496
version: 1
top level: BBa_K1054001
access: public
definition: https://synbiohub.org/public/igem/BBa_K206000/1

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2338507
display id: annotation2338507
version: 1
top level: BBa_K1054001
location: https://synbiohub.org/public/igem/BBa_K1054001/annotation2338507/range2338507/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K1054001/annotation2338507/range2338507/1
type: Range
persistent identity: range2338507
display id: range2338507
version: 1
direction: direction/reverse
top level: BBa_K1054001
start: 444
end: 572
orientation: inline

BBa_K1054000
type: SequenceAnnotation
persistent identity: annotation2338500
display id: annotation2338500
version: 1
top level: BBa_K1054001
location: https://synbiohub.org/public/igem/BBa_K1054001/annotation2338500/range2338500/1
component: BBa_K1054000

https://synbiohub.org/public/igem/BBa_K1054001/annotation2338500/range2338500/1
type: Range
persistent identity: range2338500
display id: range2338500
version: 1
direction: direction/reverse
top level: BBa_K1054001
start: 157
end: 435
orientation: inline

BBa_K206000
type: SequenceAnnotation
persistent identity: annotation2338496
display id: annotation2338496
version: 1
top level: BBa_K1054001
location: https://synbiohub.org/public/igem/BBa_K1054001/annotation2338496/range2338496/1
component: BBa_K206000

https://synbiohub.org/public/igem/BBa_K1054001/annotation2338496/range2338496/1
type: Range
persistent identity: range2338496
display id: range2338496
version: 1
direction: direction/reverse
top level: BBa_K1054001
start: 1
end: 130
orientation: inline

BBa_J61107
type: SequenceAnnotation
persistent identity: annotation2338497
display id: annotation2338497
version: 1
top level: BBa_K1054001
location: https://synbiohub.org/public/igem/BBa_K1054001/annotation2338497/range2338497/1
component: BBa_J61107

https://synbiohub.org/public/igem/BBa_K1054001/annotation2338497/range2338497/1
type: Range
persistent identity: range2338497
display id: range2338497
version: 1
direction: direction/reverse
top level: BBa_K1054001
start: 139
end: 150
orientation: inline

BBa_K112315
type: ComponentDefinition
persistent identity: BBa K112315
display id: BBa_K112315
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: {< lambda antiholin >}; adheres to Berkeley standard
creator: Aron Lau
created: 2008-10-20T11:00:00Z
modified: 2015-05-08T01:09:16Z
discontinued: false
dominant: false
experience: experience/None
group u list: _224_
m user id: 0
owner id: 2999
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: lambda antiholin with no start and stop codons. This part is actually in BBb format. ---- This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at: [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
mutable notes: PCR al0014/al0010 on pSB3C6-Bjh1503 (349bp, EcoRI/BamHI/DpnI) Sub into pBca1256 (EcoRI/BamHI, 2472+910, L) Product is pBca1256-K112315 ----------------------------------------------- al0014 Forward Biobricking of lambda antiholin with no start and stop codons CCATAGAATTCatgAGATCTaagCtgccagaaaaacatgacctg al0010 Reverse Biobricking of lambda antiholin with no start and stop codons CGttaGGATCCttgatttctaccatcttctactccggc
mutable provenance: pSB3C6-Bjh1503
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence: https://synbiohub.org/public/igem/BBa_K112315_sequence/1

BBa_K112309
type: ComponentDefinition
persistent identity: BBa K112309
display id: BBa_K112309
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: {< lambda holin >}; adheres to Berkley standard
creator: Aron Lau
created: 2008-10-20T11:00:00Z
modified: 2015-05-08T01:09:16Z
discontinued: false
dominant: false
experience: experience/None
group u list: _224_
m user id: 0
owner id: 2999
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: lambda holin with no start and stop codon in BBb format. More information about the BBb Format is available at: [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
mutable notes: PCR al0009/al0010 on pSB3C6-Bjh1502 (343bp, EcoRI/BamHI/DpnI) Sub into pBca1256 (EcoRI/BamHI, 2472+910, L) Product is pBca1256-K112309 ----------------------------------------------- al0009 Forward Biobricking of lambda holin with no start and stop codon CCATAGAATTCatgAGATCTccagaaaaacatgacctgttggcc al0010 Reverse Biobricking of lambda holin with no start and stop codon CGttaGGATCCttgatttctaccatcttctactccggc
mutable provenance: pSB3C6-Bjh1502
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence: https://synbiohub.org/public/igem/BBa_K112309_sequence/1

gpS
type: ComponentDefinition
persistent identity: BBa K836006
display id: BBa_K836006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Lysis protein S from Enterobacteria phage lambda (codon usage optimized for R. opacus)
creator: Ricardo Alvarado
created: 2012-09-19T11:00:00Z
modified: 2015-05-08T01:13:32Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1095_
m user id: 0
owner id: 11819
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This gene codifies for a protein that makes holes in the inner membrane of the bacterial cell. A Gram positive RBS (SpoVG, http://partsregistry.org/wiki/index.php?title=Part:BBa_K143021) was added at the beginning.
mutable notes: Between the RBS and the CDS a spacer consisting on a scar from a standard suffix and a preffix for CDS was inserted.
mutable provenance: The amino sequence was obtained from Uniprot (http://www.uniprot.org/uniprot/P03705#section_seq lysis protein S) and optimized for R. opacus (avoiding standard restriction sites) using the free software GeneDesigner from DNA 2.0.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
sequence annotation: SpoVG
sequence annotation: Holin
sequence annotation: Spacer
sequence: https://synbiohub.org/public/igem/BBa_K836006_sequence/1

SpoVG
type: SequenceAnnotation
persistent identity: annotation2187525
display id: annotation2187525
version: 1
top level: gpS
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K836006/annotation2187525/range2187525/1

https://synbiohub.org/public/igem/BBa_K836006/annotation2187525/range2187525/1
type: Range
persistent identity: range2187525
display id: range2187525
version: 1
direction: direction/forward
top level: gpS
start: 1
end: 12
orientation: inline

Holin
type: SequenceAnnotation
persistent identity: annotation2187528
display id: annotation2187528
version: 1
top level: gpS
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K836006/annotation2187528/range2187528/1

https://synbiohub.org/public/igem/BBa_K836006/annotation2187528/range2187528/1
type: Range
persistent identity: range2187528
display id: range2187528
version: 1
direction: direction/forward
top level: gpS
start: 19
end: 333
orientation: inline

Spacer
type: SequenceAnnotation
persistent identity: annotation2187527
display id: annotation2187527
version: 1
top level: gpS
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K836006/annotation2187527/range2187527/1

https://synbiohub.org/public/igem/BBa_K836006/annotation2187527/range2187527/1
type: Range
persistent identity: range2187527
display id: range2187527
version: 1
direction: direction/forward
top level: gpS
start: 13
end: 18
orientation: inline

BBa_K112303
type: ComponentDefinition
persistent identity: BBa K112303
display id: BBa_K112303
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: {< lambda lysozyme >} in BBb format
creator: Aron Lau
created: 2008-10-20T11:00:00Z
modified: 2015-05-08T01:09:16Z
discontinued: false
dominant: false
experience: experience/None
group u list: _224_
m user id: 0
owner id: 2999
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: lambda lysozyme with no start/stop codon in BBb format More information about the BBb Format is available at: [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
mutable notes: PCR al0003/al0004 on pSB3C6-Bjh1501 (502bp, EcoRI/BamHI/DpnI) Sub into pBca1256 (EcoRI/BamHI, 2472+910, L) Product is pBca1256-K112303 ----------------------------------------------- al0003 Forward Biobricking of lambda lysozyme with no start/stop codon CCATAGAATTCatgAGATCTgtagaaatcaataatcaacgtaaggcgttcctcg al0004 Reverse Biobricking of lambda lysozyme with no start/stop codon CGttaGGATCCtacatcaatctctctgaccgttccgc
mutable provenance: pSB3C6-Bjh1501
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence: https://synbiohub.org/public/igem/BBa_K112303_sequence/1

BBa_K112302
type: ComponentDefinition
persistent identity: BBa K112302
display id: BBa_K112302
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: {lambda lysozyme >} in BBb format
creator: Aron Lau
created: 2008-10-20T11:00:00Z
modified: 2015-05-08T01:09:16Z
discontinued: false
dominant: false
experience: experience/None
group u list: _224_
m user id: 0
owner id: 2999
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: lambda lysozyme with start codon and no stop codon in BBb format. More information about the BBb Format is available at: [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
mutable notes: PCR al0001/al0004 on pSB3C6-Bjh1501 (505bp, EcoRI/BamHI/DpnI) Sub into pBca1256 (EcoRI/BamHI, 2472+910, L) Product is pBca1256-K112302 ----------------------------------------------- al0001 Forward Biobricking of lambda lysozyme with start codon and no stop codon CCATAGAATTCatgAGATCTatggtagaaatcaataatcaacgtaaggcgttcctcg al0004 Reverse Biobricking of lambda lysozyme with start codon and no stop codon CGttaGGATCCtacatcaatctctctgaccgttccgc
mutable provenance: pSB3C6-Bjh1501
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence: https://synbiohub.org/public/igem/BBa_K112302_sequence/1

BBa_K112014
type: ComponentDefinition
persistent identity: BBa K112014
display id: BBa_K112014
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: T4 lysozyme> in BBb
creator: Bing Xia
created: 2008-09-02T11:00:00Z
modified: 2015-05-08T01:09:13Z
discontinued: false
dominant: false
experience: experience/None
group u list: _224_
m user id: 0
owner id: 2998
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: T4 lysozyme gene with a start codon, without a stop codon, in BBb format. ---- This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at: [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
mutable notes: N/A
mutable provenance: PCR bx13/bx16 on Bjh1302 (523 bp, EcoRI/BamHI/DpnI) Sub into pBca1256 (EcoRI/BamHI) Product is pBca1256-K112014 ---------------------------------------- bx13 Forward biobricking of {T4 Endolysin>} ccagtGAATTCatgAGATCTatgaatatatttgaaatgttacg bx16 Reverse biobricking of {T4 Endolysin>} cgttaGGATCCtagatttttatacgcgtcccaagtgcc
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence: https://synbiohub.org/public/igem/BBa_K112014_sequence/1

BBa_K112023
type: ComponentDefinition
persistent identity: BBa K112023
display id: BBa_K112023
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Lambda phage lysis device - no promoter
creator: Bing Xia
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:09:13Z
discontinued: false
dominant: false
group u list: _224_
m user id: 0
owner id: 2998
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This variation uses PconG6 to drive the production of antiholin. ---- This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at: [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_K112906
component: BBa_K112317
component: BBa_K112999
component: BBa_K112903
component: BBa_K112305
component: BBa_K112710
component: BBa_K112311
sequence annotation: BBa_K112999
sequence annotation: BBa_K112305
sequence annotation: BBa_K112710
sequence annotation: BBa_K112311
sequence annotation: BBa_K112317
sequence annotation: BBa_K112906
sequence annotation: BBa_K112903
sequence: https://synbiohub.org/public/igem/BBa_K112023_sequence/1

BBa_K112906
type: Component
persistent identity: component1996525
display id: component1996525
version: 1
top level: BBa_K112023
access: public
definition: https://synbiohub.org/public/igem/BBa_K112906/1

BBa_K112317
type: Component
persistent identity: component1996527
display id: component1996527
version: 1
top level: BBa_K112023
access: public
definition: BBa_K112317

BBa_K112999
type: Component
persistent identity: component1996526
display id: component1996526
version: 1
top level: BBa_K112023
access: public
definition: https://synbiohub.org/public/igem/BBa_K112999/1

BBa_K112999
type: Component
persistent identity: component1996520
display id: component1996520
version: 1
top level: BBa_K112023
access: public
definition: https://synbiohub.org/public/igem/BBa_K112999/1

BBa_K112903
type: Component
persistent identity: component1996523
display id: component1996523
version: 1
top level: BBa_K112023
access: public
definition: https://synbiohub.org/public/igem/BBa_K112903/1

BBa_K112999
type: Component
persistent identity: component1996528
display id: component1996528
version: 1
top level: BBa_K112023
access: public
definition: https://synbiohub.org/public/igem/BBa_K112999/1

BBa_K112305
type: Component
persistent identity: component1996519
display id: component1996519
version: 1
top level: BBa_K112023
access: public
definition: BBa_K112305

BBa_K112710
type: Component
persistent identity: component1996529
display id: component1996529
version: 1
top level: BBa_K112023
access: public
definition: https://synbiohub.org/public/igem/BBa_K112710/1

BBa_K112999
type: Component
persistent identity: component1996522
display id: component1996522
version: 1
top level: BBa_K112023
access: public
definition: https://synbiohub.org/public/igem/BBa_K112999/1

BBa_K112311
type: Component
persistent identity: component1996521
display id: component1996521
version: 1
top level: BBa_K112023
access: public
definition: BBa_K112311

BBa_K112999
type: Component
persistent identity: component1996524
display id: component1996524
version: 1
top level: BBa_K112023
access: public
definition: https://synbiohub.org/public/igem/BBa_K112999/1

BBa_K112999
type: SequenceAnnotation
persistent identity: annotation1996528
display id: annotation1996528
version: 1
top level: BBa_K112023
location: https://synbiohub.org/public/igem/BBa_K112023/annotation1996528/range1996528/1
component: BBa_K112999

https://synbiohub.org/public/igem/BBa_K112023/annotation1996528/range1996528/1
type: Range
persistent identity: range1996528
display id: range1996528
version: 1
direction: direction/reverse
top level: BBa_K112023
start: 1379
end: 1384
orientation: inline

BBa_K112305
type: SequenceAnnotation
persistent identity: annotation1996519
display id: annotation1996519
version: 1
top level: BBa_K112023
location: https://synbiohub.org/public/igem/BBa_K112023/annotation1996519/range1996519/1
component: BBa_K112305

https://synbiohub.org/public/igem/BBa_K112023/annotation1996519/range1996519/1
type: Range
persistent identity: range1996519
display id: range1996519
version: 1
direction: direction/reverse
top level: BBa_K112023
start: 1
end: 496
orientation: inline

BBa_K112710
type: SequenceAnnotation
persistent identity: annotation1996529
display id: annotation1996529
version: 1
top level: BBa_K112023
location: https://synbiohub.org/public/igem/BBa_K112023/annotation1996529/range1996529/1
component: BBa_K112710

https://synbiohub.org/public/igem/BBa_K112023/annotation1996529/range1996529/1
type: Range
persistent identity: range1996529
display id: range1996529
version: 1
direction: direction/reverse
top level: BBa_K112023
start: 1385
end: 1499
orientation: inline

BBa_K112999
type: SequenceAnnotation
persistent identity: annotation1996520
display id: annotation1996520
version: 1
top level: BBa_K112023
location: https://synbiohub.org/public/igem/BBa_K112023/annotation1996520/range1996520/1
component: BBa_K112999

https://synbiohub.org/public/igem/BBa_K112023/annotation1996520/range1996520/1
type: Range
persistent identity: range1996520
display id: range1996520
version: 1
direction: direction/reverse
top level: BBa_K112023
start: 497
end: 502
orientation: inline

BBa_K112311
type: SequenceAnnotation
persistent identity: annotation1996521
display id: annotation1996521
version: 1
top level: BBa_K112023
location: https://synbiohub.org/public/igem/BBa_K112023/annotation1996521/range1996521/1
component: BBa_K112311

https://synbiohub.org/public/igem/BBa_K112023/annotation1996521/range1996521/1
type: Range
persistent identity: range1996521
display id: range1996521
version: 1
direction: direction/reverse
top level: BBa_K112023
start: 503
end: 840
orientation: inline

BBa_K112999
type: SequenceAnnotation
persistent identity: annotation1996522
display id: annotation1996522
version: 1
top level: BBa_K112023
location: https://synbiohub.org/public/igem/BBa_K112023/annotation1996522/range1996522/1
component: BBa_K112999

https://synbiohub.org/public/igem/BBa_K112023/annotation1996522/range1996522/1
type: Range
persistent identity: range1996522
display id: range1996522
version: 1
direction: direction/reverse
top level: BBa_K112023
start: 841
end: 846
orientation: inline

BBa_K112317
type: SequenceAnnotation
persistent identity: annotation1996527
display id: annotation1996527
version: 1
top level: BBa_K112023
location: https://synbiohub.org/public/igem/BBa_K112023/annotation1996527/range1996527/1
component: BBa_K112317

https://synbiohub.org/public/igem/BBa_K112023/annotation1996527/range1996527/1
type: Range
persistent identity: range1996527
display id: range1996527
version: 1
direction: direction/reverse
top level: BBa_K112023
start: 1035
end: 1378
orientation: inline

BBa_K112999
type: SequenceAnnotation
persistent identity: annotation1996524
display id: annotation1996524
version: 1
top level: BBa_K112023
location: https://synbiohub.org/public/igem/BBa_K112023/annotation1996524/range1996524/1
component: BBa_K112999

https://synbiohub.org/public/igem/BBa_K112023/annotation1996524/range1996524/1
type: Range
persistent identity: range1996524
display id: range1996524
version: 1
direction: direction/reverse
top level: BBa_K112023
start: 982
end: 987
orientation: inline

BBa_K112999
type: SequenceAnnotation
persistent identity: annotation1996526
display id: annotation1996526
version: 1
top level: BBa_K112023
location: https://synbiohub.org/public/igem/BBa_K112023/annotation1996526/range1996526/1
component: BBa_K112999

https://synbiohub.org/public/igem/BBa_K112023/annotation1996526/range1996526/1
type: Range
persistent identity: range1996526
display id: range1996526
version: 1
direction: direction/reverse
top level: BBa_K112023
start: 1029
end: 1034
orientation: inline

BBa_K112906
type: SequenceAnnotation
persistent identity: annotation1996525
display id: annotation1996525
version: 1
top level: BBa_K112023
location: https://synbiohub.org/public/igem/BBa_K112023/annotation1996525/range1996525/1
component: BBa_K112906

https://synbiohub.org/public/igem/BBa_K112023/annotation1996525/range1996525/1
type: Range
persistent identity: range1996525
display id: range1996525
version: 1
direction: direction/reverse
top level: BBa_K112023
start: 988
end: 1028
orientation: inline

BBa_K112903
type: SequenceAnnotation
persistent identity: annotation1996523
display id: annotation1996523
version: 1
top level: BBa_K112023
location: https://synbiohub.org/public/igem/BBa_K112023/annotation1996523/range1996523/1
component: BBa_K112903

https://synbiohub.org/public/igem/BBa_K112023/annotation1996523/range1996523/1
type: Range
persistent identity: range1996523
display id: range1996523
version: 1
direction: direction/reverse
top level: BBa_K112023
start: 847
end: 981
orientation: inline

BBa_K358023
type: ComponentDefinition
persistent identity: BBa K358023
display id: BBa_K358023
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Lysisbox -type1- ver.2
creator: Ken Kajita
created: 2010-10-24T11:00:00Z
modified: 2015-05-08T01:12:12Z
discontinued: false
dominant: false
experience: experience/None
group u list: _455_
m user id: 0
owner id: 7170
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: This part is the same to BBa_K358020[Lysisbox], except for its constitutive promoter[BBa_J23100]. So, this plasmid induces lambda lysis cassette more than BBa_K358020. This part also contains SΔTMD1, anti-killergene, regulated by lacP. When transform this part, you must induce the SΔTMD1 highly, do not repress lacP, so that the cell lysis will be prevented. This is a long part and a lactose operon was deleted unexpectedly in our experiments. So, we recomend you that it is better to set the cultural temperature at 30C and store this part as a plasmid, not a glycerole stock.
mutable notes: We used PCR to change the constitutive promoter.
mutable provenance: BBa_K358020
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K358015
component: BBa_K358006
component: BBa_J23100
sequence annotation: BBa_K358006
sequence annotation: BBa_K358015
sequence annotation: BBa_J23100
sequence: https://synbiohub.org/public/igem/BBa_K358023_sequence/1

BBa_K358015
type: Component
persistent identity: component2230822
display id: component2230822
version: 1
top level: BBa_K358023
access: public
definition: https://synbiohub.org/public/igem/BBa_K358015/1

BBa_K358006
type: Component
persistent identity: component2230837
display id: component2230837
version: 1
top level: BBa_K358023
access: public
definition: https://synbiohub.org/public/igem/BBa_K358006/1

BBa_J23100
type: Component
persistent identity: component2230823
display id: component2230823
version: 1
top level: BBa_K358023
access: public
definition: https://synbiohub.org/public/igem/BBa_J23100/1

BBa_K358006
type: SequenceAnnotation
persistent identity: annotation2230837
display id: annotation2230837
version: 1
top level: BBa_K358023
location: https://synbiohub.org/public/igem/BBa_K358023/annotation2230837/range2230837/1
component: BBa_K358006

https://synbiohub.org/public/igem/BBa_K358023/annotation2230837/range2230837/1
type: Range
persistent identity: range2230837
display id: range2230837
version: 1
direction: direction/reverse
top level: BBa_K358023
start: 1290
end: 2699
orientation: inline

BBa_K358015
type: SequenceAnnotation
persistent identity: annotation2230822
display id: annotation2230822
version: 1
top level: BBa_K358023
location: https://synbiohub.org/public/igem/BBa_K358023/annotation2230822/range2230822/1
component: BBa_K358015

https://synbiohub.org/public/igem/BBa_K358023/annotation2230822/range2230822/1
type: Range
persistent identity: range2230822
display id: range2230822
version: 1
direction: direction/reverse
top level: BBa_K358023
start: 1
end: 1238
orientation: inline

BBa_J23100
type: SequenceAnnotation
persistent identity: annotation2230823
display id: annotation2230823
version: 1
top level: BBa_K358023
location: https://synbiohub.org/public/igem/BBa_K358023/annotation2230823/range2230823/1
component: BBa_J23100

https://synbiohub.org/public/igem/BBa_K358023/annotation2230823/range2230823/1
type: Range
persistent identity: range2230823
display id: range2230823
version: 1
direction: direction/reverse
top level: BBa_K358023
start: 1247
end: 1281
orientation: inline

BBa_K112012
type: ComponentDefinition
persistent identity: BBa K112012
display id: BBa_K112012
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: T4 lysozyme! in BBb
creator: Bing Xia
created: 2008-09-02T11:00:00Z
modified: 2015-05-08T01:09:13Z
discontinued: false
dominant: false
group u list: _224_
m user id: 0
owner id: 2998
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: T4 lysozyme gene with start and stop codons, in BBb format. ---- This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at: [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
mutable notes: N/A
mutable provenance: PCR bx13/bx14 on Bjh1302 (526 bp, EcoRI/BamHI/DpnI) Sub into pBca1256 (EcoRI/BamHI) Product is pBca1256-K112012 ---------------------------------------- bx13 Forward biobricking of {T4 Endolysin!} ccagtGAATTCatgAGATCTatgaatatatttgaaatgttacg bx14 Reverse biobricking of {T4 Endolysin!} cgttaGGATCCttatagatttttatacgcgtcccaagtgcc
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K112012_sequence/1

BBa_K112017
type: ComponentDefinition
persistent identity: BBa K112017
display id: BBa_K112017
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: rbs.T4 lysozyme! in BBb
creator: Bing Xia
created: 2008-09-02T11:00:00Z
modified: 2015-05-08T01:09:13Z
discontinued: false
dominant: false
experience: experience/None
group u list: _224_
m user id: 0
owner id: 2998
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: T4 lysozyme with a native rbs, with a stop codon, in BBb format. ---- This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at: [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
mutable notes: N/A
mutable provenance: PCR bx18/bx16 on Bjh1302 (542 bp, EcoRI/BamHI/DpnI) Sub into pBca1256 (EcoRI/BamHI) Product is pBca1256-K112017 ---------------------------------------- bx18 Forward biobricking of {rbs.T4 Endolysin!} ccagtGAATTCatgAGATCTatacttaggaggtattatgaatatatttgaaatgttac bx16 Reverse biobricking of {rbs.T4 Endolysin!} cgttaGGATCCtagatttttatacgcgtcccaagtgcc
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
sequence: https://synbiohub.org/public/igem/BBa_K112017_sequence/1

BBa_K1631023
type: ComponentDefinition
persistent identity: BBa K1631023
display id: BBa_K1631023
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Plac -rbs- Barstar -d.term
creator: Yuto Yamanaka
created: 2015-09-13T11:00:00Z
modified: 2015-09-18T06:25:53Z
discontinued: false
dominant: false
group u list: _2048_
m user id: 23567
owner id: 23567
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Barstar; Barnase immunity protein is under Plac(BBa_R0011)
mutable notes: none
mutable provenance: Assembly
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K1631007
component: BBa_R0011
sequence annotation: BBa_K1631007
sequence annotation: BBa_R0011
sequence: https://synbiohub.org/public/igem/BBa_K1631023_sequence/1

BBa_K1631007
type: Component
persistent identity: component2455759
display id: component2455759
version: 1
top level: BBa_K1631023
access: public
definition: https://synbiohub.org/public/igem/BBa_K1631007/1

BBa_R0011
type: Component
persistent identity: component2455744
display id: component2455744
version: 1
top level: BBa_K1631023
access: public
definition: https://synbiohub.org/public/igem/BBa_R0011/1

BBa_K1631007
type: SequenceAnnotation
persistent identity: annotation2455759
display id: annotation2455759
version: 1
top level: BBa_K1631023
location: https://synbiohub.org/public/igem/BBa_K1631023/annotation2455759/range2455759/1
component: BBa_K1631007

https://synbiohub.org/public/igem/BBa_K1631023/annotation2455759/range2455759/1
type: Range
persistent identity: range2455759
display id: range2455759
version: 1
direction: direction/reverse
top level: BBa_K1631023
start: 64
end: 485
orientation: inline

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation2455744
display id: annotation2455744
version: 1
top level: BBa_K1631023
location: https://synbiohub.org/public/igem/BBa_K1631023/annotation2455744/range2455744/1
component: BBa_R0011

https://synbiohub.org/public/igem/BBa_K1631023/annotation2455744/range2455744/1
type: Range
persistent identity: range2455744
display id: range2455744
version: 1
direction: direction/forward
top level: BBa_K1631023
start: 1
end: 54
orientation: inline

BBa_K112010
type: ComponentDefinition
persistent identity: BBa K112010
display id: BBa_K112010
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: a~T4 antiholin! in BBb
creator: Bing Xia
created: 2008-09-02T11:00:00Z
modified: 2015-05-08T01:09:13Z
discontinued: false
dominant: false
experience: experience/None
group u list: _224_
m user id: 0
owner id: 2998
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: T4 antiholin gene ready to have an rbs attached to the front, with a stop codon, in BBb format. ---- This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at: [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
mutable notes: N/A
mutable provenance: PCR bx11/bx8 on Bjh1303 (329 bp, EcoRI/BamHI/DpnI) Sub into pBca1256 (EcoRI/BamHI) Product is pBca1256-K112010 ---------------------------------------- bx11 Forward biobricking of {a~T4 Antiholin!} ccagtGAATTCatgAGATCTctttatggccttaaaagcaacagc bx8 Reverse biobricking of {a~T4 Antiholin!} cgttaGGATCCtcattcagtctccaatttaatgttc
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K112010_sequence/1

BBa_K112308
type: ComponentDefinition
persistent identity: BBa K112308
display id: BBa_K112308
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: {lambda holin >}; adheres to Berkeley standard
creator: Aron Lau
created: 2008-10-20T11:00:00Z
modified: 2015-05-08T01:09:16Z
discontinued: false
dominant: false
experience: experience/None
group u list: _224_
m user id: 0
owner id: 2999
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: lambda holin with start and no stop codon in BBb format. More information about the BBb Format is available at: [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
mutable notes: PCR al0007/al0010 on pSB3C6-Bjh1502 (346bp, EcoRI/BamHI/DpnI) Sub into pBca1256 (EcoRI/BamHI, 2472+910, L) Product is pBca1256-K112308 ----------------------------------------------- al0007 Forward Biobricking of lambda holin with start and no stop codon CCATAGAATTCatgAGATCTatgccagaaaaacatgacctgttgg al0010 Reverse Biobricking of lambda holin wtih start and no stop codon CGttaGGATCCttgatttctaccatcttctactccggc
mutable provenance: pSB3C6-Bjh1502
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence: https://synbiohub.org/public/igem/BBa_K112308_sequence/1

BBa_K112000
type: ComponentDefinition
persistent identity: BBa K112000
display id: BBa_K112000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: T4 holin, complete CDS, berkeley standard
creator: Bing Xia
created: 2008-09-02T11:00:00Z
modified: 2015-05-08T01:09:13Z
discontinued: false
dominant: false
experience: experience/None
group u list: _224_
m user id: 0
owner id: 2998
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: T4 holin gene, with start and stop codons. This part is actually in BBb format.
mutable provenance: PCR from existing part.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K112000_sequence/1

BBa_K733012
type: ComponentDefinition
persistent identity: BBa K733012
display id: BBa_K733012
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: xylR+PxylA+RBS+ydcE+Ptms+RBS+ydcD: Cell Growth Inhibition Device
creator: WANG, Yuqi
created: 2012-09-15T11:00:00Z
modified: 2015-05-08T01:13:06Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _979_
m user id: 0
owner id: 12444
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: Not available at this moment.
mutable notes: Not available at this moment.
mutable provenance: Not available at this moment.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K733011
component: BBa_K733010
sequence annotation: BBa_K733010
sequence annotation: BBa_K733011
sequence: https://synbiohub.org/public/igem/BBa_K733012_sequence/1

BBa_K733011
type: Component
persistent identity: component2183778
display id: component2183778
version: 1
top level: BBa_K733012
access: public
definition: https://synbiohub.org/public/igem/BBa_K733011/1

BBa_K733010
type: Component
persistent identity: component2183781
display id: component2183781
version: 1
top level: BBa_K733012
access: public
definition: https://synbiohub.org/public/igem/BBa_K733010/1

BBa_K733010
type: SequenceAnnotation
persistent identity: annotation2183781
display id: annotation2183781
version: 1
top level: BBa_K733012
location: https://synbiohub.org/public/igem/BBa_K733012/annotation2183781/range2183781/1
component: BBa_K733010

https://synbiohub.org/public/igem/BBa_K733012/annotation2183781/range2183781/1
type: Range
persistent identity: range2183781
display id: range2183781
version: 1
direction: direction/reverse
top level: BBa_K733012
start: 1773
end: 2146
orientation: inline

BBa_K733011
type: SequenceAnnotation
persistent identity: annotation2183778
display id: annotation2183778
version: 1
top level: BBa_K733012
location: https://synbiohub.org/public/igem/BBa_K733012/annotation2183778/range2183778/1
component: BBa_K733011

https://synbiohub.org/public/igem/BBa_K733012/annotation2183778/range2183778/1
type: Range
persistent identity: range2183778
display id: range2183778
version: 1
direction: direction/reverse
top level: BBa_K733012
start: 1
end: 1764
orientation: inline

BBa_K1936000
type: ComponentDefinition
persistent identity: BBa K1936000
display id: BBa_K1936000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: ePDZ-mCherry-BAX184E
creator: Marcel Dickmanns
created: 2016-10-12T11:00:00Z
modified: 2016-10-19T03:03:53Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2403_
m user id: 30478
owner id: 31678
owning group id: 9
status: status/Planning
bookmark: true
mutable description: ALERT !!!Under construction !!
mutable notes: ALERT !!!Under construction !!
mutable provenance: ALERT !!!Under construction !!
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Other
role: SO:0000110
sequence annotation: Linker
sequence annotation: ePDZ
sequence annotation: mCherry
sequence annotation: BaxS184e
sequence: https://synbiohub.org/public/igem/BBa_K1936000_sequence/1

Linker
type: SequenceAnnotation
persistent identity: annotation2521535
display id: annotation2521535
version: 1
top level: BBa_K1936000
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1936000/annotation2521535/range2521535/1

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type: Range
persistent identity: range2521535
display id: range2521535
version: 1
direction: direction/forward
top level: BBa_K1936000
start: 1318
end: 1344
orientation: inline

ePDZ
type: SequenceAnnotation
persistent identity: annotation2521533
display id: annotation2521533
version: 1
top level: BBa_K1936000
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1936000/annotation2521533/range2521533/1

https://synbiohub.org/public/igem/BBa_K1936000/annotation2521533/range2521533/1
type: Range
persistent identity: range2521533
display id: range2521533
version: 1
direction: direction/forward
top level: BBa_K1936000
start: 1
end: 582
orientation: inline

Linker
type: SequenceAnnotation
persistent identity: annotation2521532
display id: annotation2521532
version: 1
top level: BBa_K1936000
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1936000/annotation2521532/range2521532/1

https://synbiohub.org/public/igem/BBa_K1936000/annotation2521532/range2521532/1
type: Range
persistent identity: range2521532
display id: range2521532
version: 1
direction: direction/forward
top level: BBa_K1936000
start: 583
end: 609
orientation: inline

mCherry
type: SequenceAnnotation
persistent identity: annotation2521534
display id: annotation2521534
version: 1
top level: BBa_K1936000
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1936000/annotation2521534/range2521534/1

https://synbiohub.org/public/igem/BBa_K1936000/annotation2521534/range2521534/1
type: Range
persistent identity: range2521534
display id: range2521534
version: 1
direction: direction/forward
top level: BBa_K1936000
start: 610
end: 1317
orientation: inline

BaxS184e
type: SequenceAnnotation
persistent identity: annotation2521536
display id: annotation2521536
version: 1
top level: BBa_K1936000
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1936000/annotation2521536/range2521536/1

https://synbiohub.org/public/igem/BBa_K1936000/annotation2521536/range2521536/1
type: Range
persistent identity: range2521536
display id: range2521536
version: 1
direction: direction/forward
top level: BBa_K1936000
start: 1345
end: 1920
orientation: inline

BBa_K358019
type: ComponentDefinition
persistent identity: BBa K358019
display id: BBa_K358019
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Lysis cassette regulated by lacP
creator: Ken Kajita
created: 2010-10-18T11:00:00Z
modified: 2015-05-08T01:12:12Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _455_
m user id: 0
owner id: 7170
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: Lysis cassette[SRRz] is regulated by lactose promoter.
mutable notes: Because of the lysis cassette, lactose promoter must be repressed when transform it. So, we used KRX, not Top 10. This part is toxic to E.coli.
mutable provenance: biobrick, lambda phage DNA
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K358006
component: BBa_R0011
sequence annotation: BBa_K358006
sequence annotation: BBa_R0011
sequence: https://synbiohub.org/public/igem/BBa_K358019_sequence/1

BBa_K358006
type: Component
persistent identity: component2090057
display id: component2090057
version: 1
top level: BBa_K358019
access: public
definition: https://synbiohub.org/public/igem/BBa_K358006/1

BBa_R0011
type: Component
persistent identity: component2090039
display id: component2090039
version: 1
top level: BBa_K358019
access: public
definition: https://synbiohub.org/public/igem/BBa_R0011/1

BBa_K358006
type: SequenceAnnotation
persistent identity: annotation2090057
display id: annotation2090057
version: 1
top level: BBa_K358019
location: https://synbiohub.org/public/igem/BBa_K358019/annotation2090057/range2090057/1
component: BBa_K358006

https://synbiohub.org/public/igem/BBa_K358019/annotation2090057/range2090057/1
type: Range
persistent identity: range2090057
display id: range2090057
version: 1
direction: direction/reverse
top level: BBa_K358019
start: 64
end: 1473
orientation: inline

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation2090039
display id: annotation2090039
version: 1
top level: BBa_K358019
location: https://synbiohub.org/public/igem/BBa_K358019/annotation2090039/range2090039/1
component: BBa_R0011

https://synbiohub.org/public/igem/BBa_K358019/annotation2090039/range2090039/1
type: Range
persistent identity: range2090039
display id: range2090039
version: 1
direction: direction/forward
top level: BBa_K358019
start: 1
end: 54
orientation: inline

BBa_K112808
type: ComponentDefinition
persistent identity: BBa K112808
display id: BBa_K112808
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Enterobacteria phage T4 Lysis Device - no promoter
creator: Jin Huh
created: 2008-10-31T12:00:00Z
modified: 2015-05-08T01:09:19Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _224_
m user id: 0
owner id: 3025
owning group id: 171
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This is the lysis device using three genes from enterobacteria phage T4: lysozyme, holin, and antiholin. Various promoters can be installed with this device to control the lysis of bacteria. Antiholin has its own constitutive promoter to prevent the formation of holin multimers from basal expression. When the device is off, higher expression of antiholin compared to holin is required for better stability of the device.
mutable notes: [Pcon].[Antiholin].[terminator] was assembled first, and then assembled with [T4 holin].[terminator]; Constitutive expression of antiholin is required before assembling holin.
mutable provenance: Pubmed Accession Number: NC_000866 Enterobacteria phage T4
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_K112807
component: BBa_J23116
component: BBa_K112805
component: BBa_B0010
component: BBa_K112806
component: BBa_B0012
sequence annotation: BBa_K112807
sequence annotation: BBa_B0010
sequence annotation: BBa_J23116
sequence annotation: BBa_B0012
sequence annotation: BBa_K112806
sequence annotation: BBa_K112805
sequence: https://synbiohub.org/public/igem/BBa_K112808_sequence/1

BBa_K112807
type: Component
persistent identity: component1999032
display id: component1999032
version: 1
top level: BBa_K112808
access: public
definition: BBa_K112807

BBa_J23116
type: Component
persistent identity: component1999030
display id: component1999030
version: 1
top level: BBa_K112808
access: public
definition: https://synbiohub.org/public/igem/BBa_J23116/1

BBa_K112805
type: Component
persistent identity: component1999021
display id: component1999021
version: 1
top level: BBa_K112808
access: public
definition: https://synbiohub.org/public/igem/BBa_K112805/1

BBa_B0010
type: Component
persistent identity: component1999024
display id: component1999024
version: 1
top level: BBa_K112808
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_K112806
type: Component
persistent identity: component1999023
display id: component1999023
version: 1
top level: BBa_K112808
access: public
definition: BBa_K112806

BBa_B0010
type: Component
persistent identity: component1999033
display id: component1999033
version: 1
top level: BBa_K112808
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0012
type: Component
persistent identity: component1999026
display id: component1999026
version: 1
top level: BBa_K112808
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_K112807
type: SequenceAnnotation
persistent identity: annotation1999032
display id: annotation1999032
version: 1
top level: BBa_K112808
location: https://synbiohub.org/public/igem/BBa_K112808/annotation1999032/range1999032/1
component: BBa_K112807

https://synbiohub.org/public/igem/BBa_K112808/annotation1999032/range1999032/1
type: Range
persistent identity: range1999032
display id: range1999032
version: 1
direction: direction/reverse
top level: BBa_K112808
start: 1387
end: 1697
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1999024
display id: annotation1999024
version: 1
top level: BBa_K112808
location: https://synbiohub.org/public/igem/BBa_K112808/annotation1999024/range1999024/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K112808/annotation1999024/range1999024/1
type: Range
persistent identity: range1999024
display id: range1999024
version: 1
direction: direction/forward
top level: BBa_K112808
start: 1207
end: 1286
orientation: inline

BBa_J23116
type: SequenceAnnotation
persistent identity: annotation1999030
display id: annotation1999030
version: 1
top level: BBa_K112808
location: https://synbiohub.org/public/igem/BBa_K112808/annotation1999030/range1999030/1
component: BBa_J23116

https://synbiohub.org/public/igem/BBa_K112808/annotation1999030/range1999030/1
type: Range
persistent identity: range1999030
display id: range1999030
version: 1
direction: direction/reverse
top level: BBa_K112808
start: 1344
end: 1378
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1999026
display id: annotation1999026
version: 1
top level: BBa_K112808
location: https://synbiohub.org/public/igem/BBa_K112808/annotation1999026/range1999026/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K112808/annotation1999026/range1999026/1
type: Range
persistent identity: range1999026
display id: range1999026
version: 1
direction: direction/forward
top level: BBa_K112808
start: 1295
end: 1335
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1999033
display id: annotation1999033
version: 1
top level: BBa_K112808
location: https://synbiohub.org/public/igem/BBa_K112808/annotation1999033/range1999033/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K112808/annotation1999033/range1999033/1
type: Range
persistent identity: range1999033
display id: range1999033
version: 1
direction: direction/forward
top level: BBa_K112808
start: 1706
end: 1785
orientation: inline

BBa_K112806
type: SequenceAnnotation
persistent identity: annotation1999023
display id: annotation1999023
version: 1
top level: BBa_K112808
location: https://synbiohub.org/public/igem/BBa_K112808/annotation1999023/range1999023/1
component: BBa_K112806

https://synbiohub.org/public/igem/BBa_K112808/annotation1999023/range1999023/1
type: Range
persistent identity: range1999023
display id: range1999023
version: 1
direction: direction/reverse
top level: BBa_K112808
start: 685
end: 1198
orientation: inline

BBa_K112805
type: SequenceAnnotation
persistent identity: annotation1999021
display id: annotation1999021
version: 1
top level: BBa_K112808
location: https://synbiohub.org/public/igem/BBa_K112808/annotation1999021/range1999021/1
component: BBa_K112805

https://synbiohub.org/public/igem/BBa_K112808/annotation1999021/range1999021/1
type: Range
persistent identity: range1999021
display id: range1999021
version: 1
direction: direction/reverse
top level: BBa_K112808
start: 1
end: 676
orientation: inline

BBa_K112806
type: ComponentDefinition
persistent identity: BBa K112806
display id: BBa_K112806
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: [T4 endolysin]
creator: Jin Huh
created: 2008-10-31T12:00:00Z
modified: 2015-05-08T01:09:19Z
discontinued: false
dominant: true
group u list: _224_
m user id: 0
owner id: 3025
owning group id: 171
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: The lysozyme from enterobacteria phage T4 degrades peptidoglycan layer.
mutable notes: High expression of lysozyme only is toxic to bacteria.
mutable provenance: Enterobacteria phage T4 http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?val=29366675&from=66503&to=66997&view=gbwithparts
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: T4 Endolysin
sequence: https://synbiohub.org/public/igem/BBa_K112806_sequence/1

T4 Endolysin
type: SequenceAnnotation
persistent identity: annotation1999018
display id: annotation1999018
version: 1
top level: BBa_K112806
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K112806/annotation1999018/range1999018/1

https://synbiohub.org/public/igem/BBa_K112806/annotation1999018/range1999018/1
type: Range
persistent identity: range1999018
display id: range1999018
version: 1
direction: direction/forward
top level: BBa_K112806
start: 17
end: 511
orientation: inline

BBa_K1054002
type: ComponentDefinition
persistent identity: BBa K1054002
display id: BBa_K1054002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: bacteriophage PBSX holin
creator: Jiasheng Wang
created: 2013-09-09T11:00:00Z
modified: 2015-05-08T01:08:56Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1361_
m user id: 0
owner id: 15789
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: Holin is an essential protein for host lysis by bacteriophage. Its activity can by blocked by another protein called antiholin (BBa_K1054003). We can use this feature to prevent horizontal gene transfer of certain plasmid.
mutable notes: No
mutable provenance: Bacillus subtilis
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: start
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_K1054002_sequence/1

start
type: SequenceAnnotation
persistent identity: annotation2338525
display id: annotation2338525
version: 1
top level: BBa_K1054002
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1054002/annotation2338525/range2338525/1

https://synbiohub.org/public/igem/BBa_K1054002/annotation2338525/range2338525/1
type: Range
persistent identity: range2338525
display id: range2338525
version: 1
direction: direction/forward
top level: BBa_K1054002
start: 1
end: 3
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2338526
display id: annotation2338526
version: 1
top level: BBa_K1054002
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1054002/annotation2338526/range2338526/1

https://synbiohub.org/public/igem/BBa_K1054002/annotation2338526/range2338526/1
type: Range
persistent identity: range2338526
display id: range2338526
version: 1
direction: direction/forward
top level: BBa_K1054002
start: 262
end: 264
orientation: inline

BBa_K124017
type: ComponentDefinition
persistent identity: BBa K124017
display id: BBa_K124017
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Bacteriophage Lysis Cassette S105, R, and Rz
creator: Neil J. Parikh
created: 2008-10-27T12:00:00Z
modified: 2015-05-08T01:09:44Z
discontinued: false
dominant: false
experience: experience/None
group u list: _221_
m user id: 0
owner id: 3365
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: The gene cassette consists of the S105 protein (Holin), R protein (Endolysin), and Rz protein. When paired with an inducible promoter, the cassette is activated by the inducer and begins to produce the proteins which lyse the cell. This cassette contains the mutant S gene, which can be found individually as BBa_K124014. The S variation allows for faster lysis.
mutable notes: None-
mutable provenance: The DNA for this part was graciously donated to the Brown iGEM Team by Ry Young at Texas A&M University. The team then BioBricked the part for submission to the registry.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K124017_sequence/1

BBa_K1631002
type: ComponentDefinition
persistent identity: BBa K1631002
display id: BBa_K1631002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Translational unit of Colicin-E3
creator: Yuto Yamanaka
created: 2015-09-11T11:00:00Z
modified: 2015-09-14T03:43:50Z
discontinued: false
dominant: false
group u list: _2048_
m user id: 23567
owner id: 23567
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Colicin-E3 cleave 16SrRNA at a specific site. This activity is inhibited by immunity protein for Colicin-E3(BBa_). When lysis protein for Colicin-E3(BBa_) is co-expressed, Colicin-E3 is emited to environment and kill other strain. Colicin is a type of bacteriocin produced by Escherichia coli.
mutable notes: none
mutable provenance: pColE3-CA38
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
sequence annotation: BBa_B0034
sequence annotation: Colicin-E3
sequence: https://synbiohub.org/public/igem/BBa_K1631002_sequence/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2452986
display id: annotation2452986
version: 1
top level: BBa_K1631002
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1631002/annotation2452986/range2452986/1

https://synbiohub.org/public/igem/BBa_K1631002/annotation2452986/range2452986/1
type: Range
persistent identity: range2452986
display id: range2452986
version: 1
direction: direction/forward
top level: BBa_K1631002
start: 1
end: 12
orientation: inline

Colicin-E3
type: SequenceAnnotation
persistent identity: annotation2452987
display id: annotation2452987
version: 1
top level: BBa_K1631002
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1631002/annotation2452987/range2452987/1

https://synbiohub.org/public/igem/BBa_K1631002/annotation2452987/range2452987/1
type: Range
persistent identity: range2452987
display id: range2452987
version: 1
direction: direction/forward
top level: BBa_K1631002
start: 13
end: 1668
orientation: inline

BBa_K515106
type: ComponentDefinition
persistent identity: BBa K515106
display id: BBa_K515106
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: J23103 promoer - RBS B0034-RFP E1010 - Holin K112805 - endolysin K112806
creator: Atipat Patharagulpong
created: 2011-09-06T11:00:00Z
modified: 2015-05-08T01:12:32Z
discontinued: false
dominant: false
experience: experience/None
group u list: _680_
m user id: 0
owner id: 10179
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: A composite part comprising of a J23100 promotor and PA2652 translational unit encoding extracellular chemoreceptor
mutable notes: -
mutable provenance: genomic sequence
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K112805
component: BBa_E1010
component: BBa_B0034
component: BBa_K112806
component: BBa_J23103
sequence annotation: BBa_K112805
sequence annotation: BBa_B0034
sequence annotation: BBa_J23103
sequence annotation: BBa_K112806
sequence annotation: BBa_E1010
sequence: https://synbiohub.org/public/igem/BBa_K515106_sequence/1

BBa_K112805
type: Component
persistent identity: component2258432
display id: component2258432
version: 1
top level: BBa_K515106
access: public
definition: https://synbiohub.org/public/igem/BBa_K112805/1

BBa_E1010
type: Component
persistent identity: component2258430
display id: component2258430
version: 1
top level: BBa_K515106
access: public
definition: https://synbiohub.org/public/igem/BBa_E1010/1

BBa_B0034
type: Component
persistent identity: component2258427
display id: component2258427
version: 1
top level: BBa_K515106
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K112806
type: Component
persistent identity: component2258434
display id: component2258434
version: 1
top level: BBa_K515106
access: public
definition: BBa_K112806

BBa_J23103
type: Component
persistent identity: component2258425
display id: component2258425
version: 1
top level: BBa_K515106
access: public
definition: https://synbiohub.org/public/igem/BBa_J23103/1

BBa_K112805
type: SequenceAnnotation
persistent identity: annotation2258432
display id: annotation2258432
version: 1
top level: BBa_K515106
location: https://synbiohub.org/public/igem/BBa_K515106/annotation2258432/range2258432/1
component: BBa_K112805

https://synbiohub.org/public/igem/BBa_K515106/annotation2258432/range2258432/1
type: Range
persistent identity: range2258432
display id: range2258432
version: 1
direction: direction/reverse
top level: BBa_K515106
start: 776
end: 1451
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2258427
display id: annotation2258427
version: 1
top level: BBa_K515106
location: https://synbiohub.org/public/igem/BBa_K515106/annotation2258427/range2258427/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K515106/annotation2258427/range2258427/1
type: Range
persistent identity: range2258427
display id: range2258427
version: 1
direction: direction/reverse
top level: BBa_K515106
start: 44
end: 55
orientation: inline

BBa_J23103
type: SequenceAnnotation
persistent identity: annotation2258425
display id: annotation2258425
version: 1
top level: BBa_K515106
location: https://synbiohub.org/public/igem/BBa_K515106/annotation2258425/range2258425/1
component: BBa_J23103

https://synbiohub.org/public/igem/BBa_K515106/annotation2258425/range2258425/1
type: Range
persistent identity: range2258425
display id: range2258425
version: 1
direction: direction/reverse
top level: BBa_K515106
start: 1
end: 35
orientation: inline

BBa_K112806
type: SequenceAnnotation
persistent identity: annotation2258434
display id: annotation2258434
version: 1
top level: BBa_K515106
location: https://synbiohub.org/public/igem/BBa_K515106/annotation2258434/range2258434/1
component: BBa_K112806

https://synbiohub.org/public/igem/BBa_K515106/annotation2258434/range2258434/1
type: Range
persistent identity: range2258434
display id: range2258434
version: 1
direction: direction/reverse
top level: BBa_K515106
start: 1460
end: 1973
orientation: inline

BBa_E1010
type: SequenceAnnotation
persistent identity: annotation2258430
display id: annotation2258430
version: 1
top level: BBa_K515106
location: https://synbiohub.org/public/igem/BBa_K515106/annotation2258430/range2258430/1
component: BBa_E1010

https://synbiohub.org/public/igem/BBa_K515106/annotation2258430/range2258430/1
type: Range
persistent identity: range2258430
display id: range2258430
version: 1
direction: direction/reverse
top level: BBa_K515106
start: 62
end: 767
orientation: inline

BBa_K404109
type: ComponentDefinition
persistent identity: BBa K404109
display id: BBa_K404109
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Thymindine kinase (mutant TK30)
creator: Freiburg Bioware 2010
created: 2010-08-27T11:00:00Z
modified: 2015-05-08T01:12:23Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 7049
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: TK30
sequence: https://synbiohub.org/public/igem/BBa_K404109_sequence/1

TK30
type: SequenceAnnotation
persistent identity: annotation2097497
display id: annotation2097497
version: 1
top level: BBa_K404109
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404109/annotation2097497/range2097497/1

https://synbiohub.org/public/igem/BBa_K404109/annotation2097497/range2097497/1
type: Range
persistent identity: range2097497
display id: range2097497
version: 1
direction: direction/forward
top level: BBa_K404109
start: 1
end: 1020
orientation: inline

BBa_K117000
type: ComponentDefinition
persistent identity: BBa K117000
display id: BBa_K117000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Lysis gene (promotes lysis in colicin-producing bacteria strain)
creator: Nguyen Xuan Hung
created: 2008-10-06T11:00:00Z
modified: 2015-05-08T01:09:34Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _209_
m user id: 0
owner id: 2774
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: This lysis gene encodes for the lysis protein in colicin-producing strains of bacteria. Once activated, it causes the host cell to lyze. It also removes the immunity protein out of colicin, and hence, activates the endonuclease activity of the colicin.
mutable notes: to be updated
mutable provenance: to be updated
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K117000_sequence/1

BBa_K1631024
type: ComponentDefinition
persistent identity: BBa K1631024
display id: BBa_K1631024
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Colicin-E3 cassette (Strong)
creator: Yuto Yamanaka
created: 2015-09-13T11:00:00Z
modified: 2015-09-18T06:22:43Z
discontinued: false
dominant: false
group u list: _2048_
m user id: 23567
owner id: 23567
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Colicin-E3, its immunity protein, and colicin lysis protein is constitutively expressed strong.(under BBa_?J23101) When this device works, Colicin-E3 will be released to media, and E. coli strains which does not have immunity protein will be killed.
mutable notes: none
mutable provenance: Assembly
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_J23101
component: BBa_K1631009
sequence annotation: BBa_J23101
sequence annotation: BBa_K1631009
sequence: https://synbiohub.org/public/igem/BBa_K1631024_sequence/1

BBa_J23101
type: Component
persistent identity: component2455775
display id: component2455775
version: 1
top level: BBa_K1631024
access: public
definition: https://synbiohub.org/public/igem/BBa_J23101/1

BBa_K1631009
type: Component
persistent identity: component2455792
display id: component2455792
version: 1
top level: BBa_K1631024
access: public
definition: https://synbiohub.org/public/igem/BBa_K1631009/1

BBa_J23101
type: SequenceAnnotation
persistent identity: annotation2455775
display id: annotation2455775
version: 1
top level: BBa_K1631024
location: https://synbiohub.org/public/igem/BBa_K1631024/annotation2455775/range2455775/1
component: BBa_J23101

https://synbiohub.org/public/igem/BBa_K1631024/annotation2455775/range2455775/1
type: Range
persistent identity: range2455775
display id: range2455775
version: 1
direction: direction/reverse
top level: BBa_K1631024
start: 1
end: 35
orientation: inline

BBa_K1631009
type: SequenceAnnotation
persistent identity: annotation2455792
display id: annotation2455792
version: 1
top level: BBa_K1631024
location: https://synbiohub.org/public/igem/BBa_K1631024/annotation2455792/range2455792/1
component: BBa_K1631009

https://synbiohub.org/public/igem/BBa_K1631024/annotation2455792/range2455792/1
type: Range
persistent identity: range2455792
display id: range2455792
version: 1
direction: direction/reverse
top level: BBa_K1631024
start: 44
end: 2290
orientation: inline

BBa_K112015
type: ComponentDefinition
persistent identity: BBa K112015
display id: BBa_K112015
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: <T4 lysozyme> in BBb
creator: Bing Xia
created: 2008-09-02T11:00:00Z
modified: 2015-05-08T01:09:13Z
discontinued: false
dominant: false
group u list: _224_
m user id: 0
owner id: 2998
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: T4 lysozyme without start or stop codons, in BBb format. ---- This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at: [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
mutable notes: N/A
mutable provenance: PCR bx15/bx16 on Bjh1302 (520 bp, EcoRI/BamHI/DpnI) Sub into pBca1256 (EcoRI/BamHI) Product is pBca1256-K112015 ---------------------------------------- bx15 Forward biobricking of {<T4 Endolysin>} ccagtGAATTCatgAGATCTaatatatttgaaatgttacgtatagatg bx16 Reverse biobricking of {<T4 Endolysin>} cgttaGGATCCtagatttttatacgcgtcccaagtgcc
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K112015_sequence/1

BBa_K772101
type: ComponentDefinition
persistent identity: BBa K772101
display id: BBa_K772101
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Self-Destruction Kit: Endolysin + Holin
creator: Mustafa Elitok
created: 2012-09-25T11:00:00Z
modified: 2015-05-08T01:13:15Z
discontinued: false
dominant: false
group u list: _1024_
m user id: 0
owner id: 9573
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Endolysin is a lysozyme which cleaves the glycosidic bond between the C-1 of N-acetylmuramic acid (NAM) and the C-4 of N-acetylglucosamine (NAG) in the bacterial peptidoglycan (PG). This leads to the complete lysis of the cell. Holin is a protein that assembles together to form pores on inner membrane of bacteria allowing lysozyme to reach periplasm and degrade peptidoglycan layer. It is sourced from T4 bacteriophage. This composite part can be used to induce self-destruction with a regulatory device in the upstream region.
mutable notes: There is no RBS in the upstream region, so during the assemble procedure, this must be taken care of.
mutable provenance: Gene sequences of endolysin and holin are gathered from the Registry.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K112806
component: BBa_K124014
sequence annotation: BBa_K112806
sequence annotation: BBa_K124014
sequence: https://synbiohub.org/public/igem/BBa_K772101_sequence/1

BBa_K112806
type: Component
persistent identity: component2202511
display id: component2202511
version: 1
top level: BBa_K772101
access: public
definition: BBa_K112806

BBa_K124014
type: Component
persistent identity: component2202512
display id: component2202512
version: 1
top level: BBa_K772101
access: public
definition: https://synbiohub.org/public/igem/BBa_K124014/1

BBa_K112806
type: SequenceAnnotation
persistent identity: annotation2202511
display id: annotation2202511
version: 1
top level: BBa_K772101
location: https://synbiohub.org/public/igem/BBa_K772101/annotation2202511/range2202511/1
component: BBa_K112806

https://synbiohub.org/public/igem/BBa_K772101/annotation2202511/range2202511/1
type: Range
persistent identity: range2202511
display id: range2202511
version: 1
direction: direction/reverse
top level: BBa_K772101
start: 1
end: 514
orientation: inline

BBa_K124014
type: SequenceAnnotation
persistent identity: annotation2202512
display id: annotation2202512
version: 1
top level: BBa_K772101
location: https://synbiohub.org/public/igem/BBa_K772101/annotation2202512/range2202512/1
component: BBa_K124014

https://synbiohub.org/public/igem/BBa_K772101/annotation2202512/range2202512/1
type: Range
persistent identity: range2202512
display id: range2202512
version: 1
direction: direction/reverse
top level: BBa_K772101
start: 523
end: 839
orientation: inline