//function/dna
type: Collection
persistent identity: function dna
display id: function_dna
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
owned by: james
owned by: myers
member: BBa_K332033
member: occupyDNAP
member: BBa_I716462
member: BBa_K112120
member: ThiE
member: mt ori7
member: message
member: key
member: BBa_K112121
member: N4 gp8
member: BBa_K364101
member: BBa_K332032
member: BBa_K283017
member: BBa_K332031

BBa_K112121
type: ComponentDefinition
persistent identity: BBa K112121
display id: BBa_K112121
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: BglII endonuclease with ribosome binding site
creator: Madhvi Venkatesh
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:09:14Z
discontinued: false
dominant: false
experience: experience/None
group u list: _224_
m user id: 0
owner id: 3023
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: BglII nuclease with ribosome binding site and stop codon
mutable notes: This part is built in Biobrick version 2.0. In this format, parts are flanked by BglII and BamHI restriction sites. --- PCR mv032/mv010 on pBca9145-Bca1147 (722bp, EcoRI/BamHI/DpnI) Sub into pBca1256 (EcoRI/BamHI, 2472+910, L) Product is pBca1256-K112121 ----------------------------------------------- mv032 Forward Biobricking of rbs.r.BglII! cgataGAATTCatgAGATCTtggaaggagacactcataatgaagattgatataacggactataaccatgc mv010 Reverse Biobricking of r.BglII! cgttaGGATCCttaatatgtcacgattgttcctc
mutable provenance: PCR mv032/mv010 on pBca9145-Bca1147 (722bp, EcoRI/BamHI/DpnI) Sub into pBca1256 (EcoRI/BamHI, 2472+910, L) Product is pBca1256-K112121
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
sequence: https://synbiohub.org/public/igem/BBa_K112121_sequence/1

BBa_K364101
type: ComponentDefinition
persistent identity: BBa K364101
display id: BBa_K364101
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Zinc Finger Protein AAA
creator: Ophir Keret
created: 2010-07-26T11:00:00Z
modified: 2015-05-08T01:12:13Z
discontinued: false
dominant: false
experience: experience/None
group u list: _486_
m user id: 0
owner id: 6746
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This part codes for a Cys2His2 zinc finger protein to be used in assembly of zinc finger polymers (to be continued).
mutable notes: Special RFC needs to be constructed for this purpose
mutable provenance: Completely artificial
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000110
role: partType/Temporary
sequence annotation: Recognition Alpha Helix
sequence: https://synbiohub.org/public/igem/BBa_K364101_sequence/1

Recognition Alpha Helix
type: SequenceAnnotation
persistent identity: annotation2077781
display id: annotation2077781
version: 1
top level: BBa_K364101
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K364101/annotation2077781/range2077781/1

https://synbiohub.org/public/igem/BBa_K364101/annotation2077781/range2077781/1
type: Range
persistent identity: range2077781
display id: range2077781
version: 1
direction: direction/forward
top level: BBa_K364101
start: 34
end: 54
orientation: inline

BBa_K112120
type: ComponentDefinition
persistent identity: BBa K112120
display id: BBa_K112120
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: rbs.BamHI
creator: Bing Xia
created: 2008-10-20T11:00:00Z
modified: 2015-05-08T01:09:14Z
discontinued: false
dominant: false
experience: experience/None
group u list: _224_
m user id: 0
owner id: 2998
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: ---- This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at: [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
mutable provenance: PCR mv031/mv006 on pBca9145-I716210 (692bp, EcoRI/BamHI/DpnI) Sub into pBca1256 (EcoRI/BamHI, 2472+910, L) Product is pBca1256-K112120 ----------------------------------------------- mv031 Forward Biobricking of rbs.r.BamH1! cgataGAATTCatgAGATCTtaagtgggggcaaattagaatggaagtagaaaaagagtttattactgatgaagc mv006 Reverse Biobricking of r.BamH1! cgttaGGATCCctatttgttttcaactttatctttcc
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
sequence: https://synbiohub.org/public/igem/BBa_K112120_sequence/1

BBa_K332031
type: ComponentDefinition
persistent identity: BBa K332031
display id: BBa_K332031
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Constitutive promoter + 37℃ induced RBS + tetR + double terminator
creator: KUO, CHIA-CHUN
created: 2010-10-21T11:00:00Z
modified: 2015-05-08T01:12:05Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _452_
m user id: 0
owner id: 7059
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: we took advantage of the interaction between 37?? C induced RBS, tetR gene and pTet promoter to create the temperature regulating circuit. The following is a description of our prescribed strategy. First, we use the constitutive promoter, 37?? C induced RBS (K115002), the tetR gene and terminators. We will call this part the ???Regulator???. We speculate that when the ???Regulator??? and ???Production??? sections are combined, we can achieve a high bacteria growth rate and close to no target protein producing rate at T>37??C, and low bacteria growth rate and high target protein producing rate at T<37??C.
mutable notes: To regulate following gene by temperature
mutable provenance: iGEM08_TUDelft
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_J23101
component: BBa_K115002
component: BBa_C0040
component: BBa_J61048
sequence annotation: BBa_C0040
sequence annotation: BBa_J23101
sequence annotation: BBa_J61048
sequence annotation: BBa_K115002
sequence: https://synbiohub.org/public/igem/BBa_K332031_sequence/1

BBa_J23101
type: Component
persistent identity: component2250397
display id: component2250397
version: 1
top level: BBa_K332031
access: public
definition: https://synbiohub.org/public/igem/BBa_J23101/1

BBa_K115002
type: Component
persistent identity: component2250402
display id: component2250402
version: 1
top level: BBa_K332031
access: public
definition: https://synbiohub.org/public/igem/BBa_K115002/1

BBa_C0040
type: Component
persistent identity: component2250406
display id: component2250406
version: 1
top level: BBa_K332031
access: public
definition: https://synbiohub.org/public/igem/BBa_C0040/1

BBa_J61048
type: Component
persistent identity: component2250407
display id: component2250407
version: 1
top level: BBa_K332031
access: public
definition: https://synbiohub.org/public/igem/BBa_J61048/1

BBa_C0040
type: SequenceAnnotation
persistent identity: annotation2250406
display id: annotation2250406
version: 1
top level: BBa_K332031
location: https://synbiohub.org/public/igem/BBa_K332031/annotation2250406/range2250406/1
component: BBa_C0040

https://synbiohub.org/public/igem/BBa_K332031/annotation2250406/range2250406/1
type: Range
persistent identity: range2250406
display id: range2250406
version: 1
direction: direction/reverse
top level: BBa_K332031
start: 102
end: 786
orientation: inline

BBa_J23101
type: SequenceAnnotation
persistent identity: annotation2250397
display id: annotation2250397
version: 1
top level: BBa_K332031
location: https://synbiohub.org/public/igem/BBa_K332031/annotation2250397/range2250397/1
component: BBa_J23101

https://synbiohub.org/public/igem/BBa_K332031/annotation2250397/range2250397/1
type: Range
persistent identity: range2250397
display id: range2250397
version: 1
direction: direction/reverse
top level: BBa_K332031
start: 1
end: 35
orientation: inline

BBa_J61048
type: SequenceAnnotation
persistent identity: annotation2250407
display id: annotation2250407
version: 1
top level: BBa_K332031
location: https://synbiohub.org/public/igem/BBa_K332031/annotation2250407/range2250407/1
component: BBa_J61048

https://synbiohub.org/public/igem/BBa_K332031/annotation2250407/range2250407/1
type: Range
persistent identity: range2250407
display id: range2250407
version: 1
direction: direction/reverse
top level: BBa_K332031
start: 795
end: 907
orientation: inline

BBa_K115002
type: SequenceAnnotation
persistent identity: annotation2250402
display id: annotation2250402
version: 1
top level: BBa_K332031
location: https://synbiohub.org/public/igem/BBa_K332031/annotation2250402/range2250402/1
component: BBa_K115002

https://synbiohub.org/public/igem/BBa_K332031/annotation2250402/range2250402/1
type: Range
persistent identity: range2250402
display id: range2250402
version: 1
direction: direction/reverse
top level: BBa_K332031
start: 44
end: 95
orientation: inline

message
type: ComponentDefinition
persistent identity: BBa K1930001
display id: BBa_K1930001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Encrypted message sequence
creator: Barbora Waclawikova
created: 2016-10-15T11:00:00Z
modified: 2016-10-16T04:40:07Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2397_
m user id: 29748
owner id: 29748
owning group id: 9
status: status/Planning
bookmark: false
mutable description: The iGEM team Groningen 2016 worked on bioencryption in order to safely store data in DNA. In our project we were mainly working with two sequences of DNA which were the encrypted message sequence and the encryption key sequence. The message sequence was created by our software. It is therefore artificial DNA. We ordered the encrypted message sequence as gBlock from IDT (Integrated DNA technologies).
mutable notes: The iGEM team Groningen 2016 worked on bioencryption in order to safely store data in DNA. In our project we were mainly working with two sequences of DNA which were the encrypted message sequence and the encryption key sequence. The message sequence was created by our software. It is therefore artificial DNA. We ordered the encrypted message sequence as gBlock from IDT (Integrated DNA technologies).
mutable provenance: Created by our CryptoGErM software. http://2016.igem.org/Team:Groningen/Encoding
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Other
role: SO:0000110
sequence annotation: message sequence
sequence: https://synbiohub.org/public/igem/BBa_K1930001_sequence/1

message sequence
type: SequenceAnnotation
persistent identity: annotation2513401
display id: annotation2513401
version: 1
top level: message
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1930001/annotation2513401/range2513401/1

https://synbiohub.org/public/igem/BBa_K1930001/annotation2513401/range2513401/1
type: Range
persistent identity: range2513401
display id: range2513401
version: 1
direction: direction/forward
top level: message
start: 1
end: 555
orientation: inline

key
type: ComponentDefinition
persistent identity: BBa K1930000
display id: BBa_K1930000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Encryption key sequence
creator: Eike Mahlandt
created: 2016-10-06T11:00:00Z
modified: 2016-10-16T04:40:29Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2397_
m user id: 29748
owner id: 29748
owning group id: 9
status: status/Planning
bookmark: true
mutable description: key
mutable notes: key
mutable provenance: key
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Other
role: SO:0000110
sequence annotation: key sequence
sequence: https://synbiohub.org/public/igem/BBa_K1930000_sequence/1

key sequence
type: SequenceAnnotation
persistent identity: annotation2513258
display id: annotation2513258
version: 1
top level: key
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1930000/annotation2513258/range2513258/1

https://synbiohub.org/public/igem/BBa_K1930000/annotation2513258/range2513258/1
type: Range
persistent identity: range2513258
display id: range2513258
version: 1
direction: direction/forward
top level: key
start: 1
end: 143
orientation: inline

mt ori7
type: ComponentDefinition
persistent identity: BBa K809002
display id: BBa_K809002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: ori7 region of mtDNA
creator: Qiwu XU
created: 2012-09-19T11:00:00Z
modified: 2015-05-08T01:13:26Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1066_
m user id: 0
owner id: 11943
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: ori 7 region of Saccharomyces cerevisiae mtDNA, works as replication initial site.
mutable notes: none
mutable provenance: NCBI database of Saccharomyces cerevisiae's genome
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Other
role: SO:0000110
sequence annotation: ori7
sequence: https://synbiohub.org/public/igem/BBa_K809002_sequence/1

ori7
type: SequenceAnnotation
persistent identity: annotation2188522
display id: annotation2188522
version: 1
top level: mt ori7
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K809002/annotation2188522/range2188522/1

https://synbiohub.org/public/igem/BBa_K809002/annotation2188522/range2188522/1
type: Range
persistent identity: range2188522
display id: range2188522
version: 1
direction: direction/forward
top level: mt ori7
start: 1
end: 1
orientation: inline

ori7
type: SequenceAnnotation
persistent identity: annotation2188523
display id: annotation2188523
version: 1
top level: mt ori7
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K809002/annotation2188523/range2188523/1

https://synbiohub.org/public/igem/BBa_K809002/annotation2188523/range2188523/1
type: Range
persistent identity: range2188523
display id: range2188523
version: 1
direction: direction/forward
top level: mt ori7
start: 378
end: 378
orientation: inline

BBa_K283017
type: ComponentDefinition
persistent identity: BBa K283017
display id: BBa_K283017
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: prosseA-rbs-T7 RNAP (Backbone: pSB1A2)
creator: Shi Lei, Nelson Chu, W Ling
created: 2009-10-12T11:00:00Z
modified: 2015-05-08T01:11:47Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _383_
m user id: 0
owner id: 5494
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: T7polymerase coding region under a prosseA promoter
mutable notes: BBa_K283017
mutable provenance: The T7polymerase, prosseA promoter is self-desgined The rbs is from the registry
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K283038
component: BBa_B0034
sequence annotation: BBa_K283038
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K283017_sequence/1

BBa_K283038
type: Component
persistent identity: component2047783
display id: component2047783
version: 1
top level: BBa_K283017
access: public
definition: https://synbiohub.org/public/igem/BBa_K283038/1

BBa_B0034
type: Component
persistent identity: component2047785
display id: component2047785
version: 1
top level: BBa_K283017
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K283038
type: SequenceAnnotation
persistent identity: annotation2047783
display id: annotation2047783
version: 1
top level: BBa_K283017
location: https://synbiohub.org/public/igem/BBa_K283017/annotation2047783/range2047783/1
component: BBa_K283038

https://synbiohub.org/public/igem/BBa_K283017/annotation2047783/range2047783/1
type: Range
persistent identity: range2047783
display id: range2047783
version: 1
direction: direction/reverse
top level: BBa_K283017
start: 1
end: 500
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2047785
display id: annotation2047785
version: 1
top level: BBa_K283017
location: https://synbiohub.org/public/igem/BBa_K283017/annotation2047785/range2047785/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K283017/annotation2047785/range2047785/1
type: Range
persistent identity: range2047785
display id: range2047785
version: 1
direction: direction/reverse
top level: BBa_K283017
start: 509
end: 520
orientation: inline

BBa_I716462
type: ComponentDefinition
persistent identity: BBa I716462
display id: BBa_I716462
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: {AraC-Pbad}{rbs1-A}{ATG}{BamHI no ATG}
creator: Samantha Liang
created: 2007-10-22T11:00:00Z
modified: 2015-08-31T04:07:52Z
discontinued: false
dominant: false
group u list: _110_
m user id: 0
owner id: 933
owning group id: 52
sample status: In stock
status: status/Available
bookmark: false
mutable description: {AraC-Pbad}{rbs1-A}{ATG}{BamHI no ATG}
mutable notes: This part is in biobricks 2.0 with BamHI and BglII sites instead of PstI and SpeI sites
mutable provenance: I716450 + I716210
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Other
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_I716462_sequence/1

BBa_K332032
type: ComponentDefinition
persistent identity: BBa K332032
display id: BBa_K332032
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Constitutive promoter+37℃ induced RBS+tetR+double terminator+Ptet+RBS+GFP+double terminator
creator: KUO, CHIA-CHUN
created: 2010-10-21T11:00:00Z
modified: 2015-05-08T01:12:05Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _452_
m user id: 0
owner id: 7059
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: We want to utilize a temperature-regulated mechanism to control the expression period of a crystal protein. Therefore, we took advantage of the interaction between 37?? C induced RBS, tetR gene and pTet promoter to create the temperature regulating circuit. The following is a description of our prescribed strategy. First, we use the constitutive promoter, 37?? C induced RBS (K115002), the tetR gene and terminators. We will call this part the ???Regulator???.
mutable notes: none
mutable provenance: none
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0034
component: BBa_E0040
component: BBa_J61048
component: BBa_K332031
component: BBa_R0040
sequence annotation: BBa_R0040
sequence annotation: BBa_E0040
sequence annotation: BBa_B0034
sequence annotation: BBa_K332031
sequence annotation: BBa_J61048
sequence: https://synbiohub.org/public/igem/BBa_K332032_sequence/1

BBa_B0034
type: Component
persistent identity: component2273253
display id: component2273253
version: 1
top level: BBa_K332032
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_E0040
type: Component
persistent identity: component2273255
display id: component2273255
version: 1
top level: BBa_K332032
access: public
definition: https://synbiohub.org/public/igem/BBa_E0040/1

BBa_J61048
type: Component
persistent identity: component2273256
display id: component2273256
version: 1
top level: BBa_K332032
access: public
definition: https://synbiohub.org/public/igem/BBa_J61048/1

BBa_K332031
type: Component
persistent identity: component2273246
display id: component2273246
version: 1
top level: BBa_K332032
access: public
definition: BBa_K332031

BBa_R0040
type: Component
persistent identity: component2273247
display id: component2273247
version: 1
top level: BBa_K332032
access: public
definition: https://synbiohub.org/public/igem/BBa_R0040/1

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation2273247
display id: annotation2273247
version: 1
top level: BBa_K332032
location: https://synbiohub.org/public/igem/BBa_K332032/annotation2273247/range2273247/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_K332032/annotation2273247/range2273247/1
type: Range
persistent identity: range2273247
display id: range2273247
version: 1
direction: direction/forward
top level: BBa_K332032
start: 916
end: 969
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation2273255
display id: annotation2273255
version: 1
top level: BBa_K332032
location: https://synbiohub.org/public/igem/BBa_K332032/annotation2273255/range2273255/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_K332032/annotation2273255/range2273255/1
type: Range
persistent identity: range2273255
display id: range2273255
version: 1
direction: direction/reverse
top level: BBa_K332032
start: 996
end: 1715
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2273253
display id: annotation2273253
version: 1
top level: BBa_K332032
location: https://synbiohub.org/public/igem/BBa_K332032/annotation2273253/range2273253/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K332032/annotation2273253/range2273253/1
type: Range
persistent identity: range2273253
display id: range2273253
version: 1
direction: direction/reverse
top level: BBa_K332032
start: 978
end: 989
orientation: inline

BBa_K332031
type: SequenceAnnotation
persistent identity: annotation2273246
display id: annotation2273246
version: 1
top level: BBa_K332032
location: https://synbiohub.org/public/igem/BBa_K332032/annotation2273246/range2273246/1
component: BBa_K332031

https://synbiohub.org/public/igem/BBa_K332032/annotation2273246/range2273246/1
type: Range
persistent identity: range2273246
display id: range2273246
version: 1
direction: direction/reverse
top level: BBa_K332032
start: 1
end: 907
orientation: inline

BBa_J61048
type: SequenceAnnotation
persistent identity: annotation2273256
display id: annotation2273256
version: 1
top level: BBa_K332032
location: https://synbiohub.org/public/igem/BBa_K332032/annotation2273256/range2273256/1
component: BBa_J61048

https://synbiohub.org/public/igem/BBa_K332032/annotation2273256/range2273256/1
type: Range
persistent identity: range2273256
display id: range2273256
version: 1
direction: direction/reverse
top level: BBa_K332032
start: 1724
end: 1836
orientation: inline

ThiE
type: ComponentDefinition
persistent identity: BBa K558009
display id: BBa_K558009
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Thiamin monophosphate pyrophosphorylase
creator: Laura Colgin, Megan Tabor
created: 2011-09-22T11:00:00Z
modified: 2015-05-08T01:12:41Z
discontinued: false
dominant: false
experience: experience/None
group u list: _726_
m user id: 0
owner id: 8801
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Coding region of Thiamine E from synechocystis
mutable notes: coming soon
mutable provenance: Genomic DNA
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: start
sequence annotation: ThiE coding region
sequence: https://synbiohub.org/public/igem/BBa_K558009_sequence/1

start
type: SequenceAnnotation
persistent identity: annotation2144392
display id: annotation2144392
version: 1
top level: ThiE
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K558009/annotation2144392/range2144392/1

https://synbiohub.org/public/igem/BBa_K558009/annotation2144392/range2144392/1
type: Range
persistent identity: range2144392
display id: range2144392
version: 1
direction: direction/forward
top level: ThiE
start: 1
end: 3
orientation: inline

ThiE coding region
type: SequenceAnnotation
persistent identity: annotation2144393
display id: annotation2144393
version: 1
top level: ThiE
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K558009/annotation2144393/range2144393/1

https://synbiohub.org/public/igem/BBa_K558009/annotation2144393/range2144393/1
type: Range
persistent identity: range2144393
display id: range2144393
version: 1
direction: direction/forward
top level: ThiE
start: 1
end: 1032
orientation: inline

BBa_K332033
type: ComponentDefinition
persistent identity: BBa K332033
display id: BBa_K332033
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: 37℃ induced RBS + tetR + double terminator
creator: KUO, CHIA-CHUN
created: 2010-10-23T11:00:00Z
modified: 2015-05-08T01:12:05Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _452_
m user id: 0
owner id: 7059
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: It is a transcriptional unit including 37℃ induced RBS + tetR + double terminator. It can be added various promoter to adapt your conditions.
mutable notes: To regulate following genes by temperature. To maintain cells at 37?? C ↑ : the tetR protein be generated and repress Ptet's circuit. To release cells at 37?? C ↓ : the tetR protein not be generated and do not repress Ptet's circuit.
mutable provenance: the 37℃ induced RBS cames from iGEM08_TUDelft
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_C0040
component: BBa_K115002
component: BBa_J61048
sequence annotation: BBa_C0040
sequence annotation: BBa_K115002
sequence annotation: BBa_J61048
sequence: https://synbiohub.org/public/igem/BBa_K332033_sequence/1

BBa_C0040
type: Component
persistent identity: component2247615
display id: component2247615
version: 1
top level: BBa_K332033
access: public
definition: https://synbiohub.org/public/igem/BBa_C0040/1

BBa_K115002
type: Component
persistent identity: component2247611
display id: component2247611
version: 1
top level: BBa_K332033
access: public
definition: https://synbiohub.org/public/igem/BBa_K115002/1

BBa_J61048
type: Component
persistent identity: component2247616
display id: component2247616
version: 1
top level: BBa_K332033
access: public
definition: https://synbiohub.org/public/igem/BBa_J61048/1

BBa_C0040
type: SequenceAnnotation
persistent identity: annotation2247615
display id: annotation2247615
version: 1
top level: BBa_K332033
location: https://synbiohub.org/public/igem/BBa_K332033/annotation2247615/range2247615/1
component: BBa_C0040

https://synbiohub.org/public/igem/BBa_K332033/annotation2247615/range2247615/1
type: Range
persistent identity: range2247615
display id: range2247615
version: 1
direction: direction/reverse
top level: BBa_K332033
start: 59
end: 743
orientation: inline

BBa_K115002
type: SequenceAnnotation
persistent identity: annotation2247611
display id: annotation2247611
version: 1
top level: BBa_K332033
location: https://synbiohub.org/public/igem/BBa_K332033/annotation2247611/range2247611/1
component: BBa_K115002

https://synbiohub.org/public/igem/BBa_K332033/annotation2247611/range2247611/1
type: Range
persistent identity: range2247611
display id: range2247611
version: 1
direction: direction/reverse
top level: BBa_K332033
start: 1
end: 52
orientation: inline

BBa_J61048
type: SequenceAnnotation
persistent identity: annotation2247616
display id: annotation2247616
version: 1
top level: BBa_K332033
location: https://synbiohub.org/public/igem/BBa_K332033/annotation2247616/range2247616/1
component: BBa_J61048

https://synbiohub.org/public/igem/BBa_K332033/annotation2247616/range2247616/1
type: Range
persistent identity: range2247616
display id: range2247616
version: 1
direction: direction/reverse
top level: BBa_K332033
start: 752
end: 864
orientation: inline

occupyDNAP
type: ComponentDefinition
persistent identity: BBa M36549
display id: BBa_M36549
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PoPS --> N4 gp8 (DNA replication inhibitor)
creator: Rashmi Sharma, Bryce Bajar, Kendrick Wang
created: 2011-12-08T12:00:00Z
modified: 2015-05-08T01:14:05Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _848_
m user id: 0
owner id: 11054
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: to be added
mutable notes: to be added
mutable provenance: to be added
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0032
component: BBa_M36548
component: BBa_M36292
sequence annotation: BBa_M36548
sequence annotation: BBa_M36292
sequence annotation: BBa_B0032
sequence: https://synbiohub.org/public/igem/BBa_M36549_sequence/1

BBa_B0032
type: Component
persistent identity: component2166548
display id: component2166548
version: 1
top level: occupyDNAP
access: public
definition: https://synbiohub.org/public/igem/BBa_B0032/1

BBa_M36548
type: Component
persistent identity: component2166551
display id: component2166551
version: 1
top level: occupyDNAP
access: public
definition: N4 gp8

BBa_M36292
type: Component
persistent identity: component2166552
display id: component2166552
version: 1
top level: occupyDNAP
access: public
definition: https://synbiohub.org/public/igem/BBa_M36292/1

BBa_M36548
type: SequenceAnnotation
persistent identity: annotation2166551
display id: annotation2166551
version: 1
top level: occupyDNAP
location: https://synbiohub.org/public/igem/BBa_M36549/annotation2166551/range2166551/1
component: BBa_M36548

https://synbiohub.org/public/igem/BBa_M36549/annotation2166551/range2166551/1
type: Range
persistent identity: range2166551
display id: range2166551
version: 1
direction: direction/reverse
top level: occupyDNAP
start: 20
end: 235
orientation: inline

BBa_M36292
type: SequenceAnnotation
persistent identity: annotation2166552
display id: annotation2166552
version: 1
top level: occupyDNAP
location: https://synbiohub.org/public/igem/BBa_M36549/annotation2166552/range2166552/1
component: BBa_M36292

https://synbiohub.org/public/igem/BBa_M36549/annotation2166552/range2166552/1
type: Range
persistent identity: range2166552
display id: range2166552
version: 1
direction: direction/reverse
top level: occupyDNAP
start: 244
end: 325
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation2166548
display id: annotation2166548
version: 1
top level: occupyDNAP
location: https://synbiohub.org/public/igem/BBa_M36549/annotation2166548/range2166548/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_M36549/annotation2166548/range2166548/1
type: Range
persistent identity: range2166548
display id: range2166548
version: 1
direction: direction/forward
top level: occupyDNAP
start: 1
end: 13
orientation: inline

N4 gp8
type: ComponentDefinition
persistent identity: BBa M36548
display id: BBa_M36548
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: DNA Polymerase Inhibitor (via N4 coliphage gp8)
creator: Rashmi Sharma, Bryce Bajar, Kendrick Wang
created: 2011-12-07T12:00:00Z
modified: 2015-05-08T01:14:05Z
discontinued: false
dominant: false
experience: experience/None
group u list: _848_
m user id: 0
owner id: 11092
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: to be added
mutable notes: to be added
mutable provenance: to be added
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: N4 gp8
sequence: https://synbiohub.org/public/igem/BBa_M36548_sequence/1

N4 gp8
type: SequenceAnnotation
persistent identity: annotation2166525
display id: annotation2166525
version: 1
top level: N4 gp8
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_M36548/annotation2166525/range2166525/1

https://synbiohub.org/public/igem/BBa_M36548/annotation2166525/range2166525/1
type: Range
persistent identity: range2166525
display id: range2166525
version: 1
direction: direction/forward
top level: N4 gp8
start: 1
end: 216
orientation: inline