//function/immunology
type: Collection
persistent identity: function immunology
display id: function_immunology
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
owned by: james
owned by: myers
member: BBa_J52019
member: BBa_I712021
member: BBa_I712017
member: BBa_I712002
member: BBa_I712012
member: BBa_K258002
member: BBa_K133057
member: BBa_J52021
member: BBa_K133046
member: BBa_I712010
member: BBa_K133016
member: BBa_J52026
member: scFv
member: BBa_K133010
member: BBa_J52013
member: BBa_J52011
member: BBa_I712024
member: BBa_J52035

BBa_I712021
type: ComponentDefinition
persistent identity: BBa I712021
display id: BBa_I712021
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Interferon beta
creator: Anja Korenčič
created: 2007-10-21T11:00:00Z
modified: 2015-08-31T04:07:46Z
discontinued: false
dominant: false
group u list: _130_
m user id: 0
owner id: 1846
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Homo sapiens interferon beta.
mutable notes: PstI restriction site was mutated. Includes start and stop codon.
mutable provenance: Part comes from Origene human cDNA clone (NM_002176.2).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_I712021_sequence/1

BBa_J52013
type: ComponentDefinition
persistent identity: BBa J52013
display id: BBa_J52013
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: dnMyD88-linker-Rluc-linker-PEST191
creator: Monika Ciglic
created: 2006-10-13T11:00:00Z
modified: 2015-08-31T03:54:04Z
discontinued: false
dominant: false
group u list: _80_
m user id: 0
owner id: 800
owning group id: 80
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: dnMyD88-linker-Rluc-linker-PEST191 is a part where dnMyD88 (very important protein in signaling cascade through TLR4 receptor) is linked to Rluc (reporter) and PEST191 (degradation tag). Both linkers are made of six aminoacids.
mutable notes: dnMyD88-linker-Rluc-linker-PEST191 was made by using PCR ligation technique. It was difficult since we had some problems with primers. This part is cloned in BioBrick vector pSB1AK3.
mutable provenance: -
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Rluc
sequence annotation: dnMyD88
sequence annotation: PEST191
sequence annotation: linker
sequence annotation: dnMyD88-linker-Rluc-linker-PEST191
sequence: https://synbiohub.org/public/igem/BBa_J52013_sequence/1

Rluc
type: SequenceAnnotation
persistent identity: annotation1902842
display id: annotation1902842
version: 1
top level: BBa_J52013
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J52013/annotation1902842/range1902842/1

https://synbiohub.org/public/igem/BBa_J52013/annotation1902842/range1902842/1
type: Range
persistent identity: range1902842
display id: range1902842
version: 1
direction: direction/forward
top level: BBa_J52013
start: 436
end: 1368
orientation: inline

dnMyD88
type: SequenceAnnotation
persistent identity: annotation1902840
display id: annotation1902840
version: 1
top level: BBa_J52013
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J52013/annotation1902840/range1902840/1

https://synbiohub.org/public/igem/BBa_J52013/annotation1902840/range1902840/1
type: Range
persistent identity: range1902840
display id: range1902840
version: 1
direction: direction/forward
top level: BBa_J52013
start: 1
end: 417
orientation: inline

PEST191
type: SequenceAnnotation
persistent identity: annotation1902844
display id: annotation1902844
version: 1
top level: BBa_J52013
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_J52013/annotation1902844/range1902844/1

https://synbiohub.org/public/igem/BBa_J52013/annotation1902844/range1902844/1
type: Range
persistent identity: range1902844
display id: range1902844
version: 1
direction: direction/forward
top level: BBa_J52013
start: 1387
end: 1872
orientation: inline

linker
type: SequenceAnnotation
persistent identity: annotation1902843
display id: annotation1902843
version: 1
top level: BBa_J52013
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_J52013/annotation1902843/range1902843/1

https://synbiohub.org/public/igem/BBa_J52013/annotation1902843/range1902843/1
type: Range
persistent identity: range1902843
display id: range1902843
version: 1
direction: direction/forward
top level: BBa_J52013
start: 1369
end: 1386
orientation: inline

dnMyD88-linker-Rluc-linker-PEST191
type: SequenceAnnotation
persistent identity: annotation1902848
display id: annotation1902848
version: 1
top level: BBa_J52013
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_J52013/annotation1902848/range1902848/1

https://synbiohub.org/public/igem/BBa_J52013/annotation1902848/range1902848/1
type: Range
persistent identity: range1902848
display id: range1902848
version: 1
direction: direction/forward
top level: BBa_J52013
start: 1
end: 1872
orientation: inline

linker
type: SequenceAnnotation
persistent identity: annotation1902841
display id: annotation1902841
version: 1
top level: BBa_J52013
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_J52013/annotation1902841/range1902841/1

https://synbiohub.org/public/igem/BBa_J52013/annotation1902841/range1902841/1
type: Range
persistent identity: range1902841
display id: range1902841
version: 1
direction: direction/forward
top level: BBa_J52013
start: 418
end: 435
orientation: inline

BBa_K258002
type: ComponentDefinition
persistent identity: BBa K258002
display id: BBa_K258002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Granulysin, a T Cell Product,Kills Bacteria by Altering Membrane Permeability
creator: Ozkan IS
created: 2009-10-02T11:00:00Z
modified: 2016-02-01T12:59:26Z
discontinued: false
dominant: true
experience: experience/None
group u list: _358_
m user id: 4206
owner id: 4260
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: Granulysin, a protein located in the acidic granules of human NK cells and cytotoxic T cells, has microbial activity against a broad spectrum of microbial pathogens. The substance released by cytotoxic T cells (CD8) when attached to infected body cells. It creates holes in the target cell membrane and destroy it. That is,Granulysin induces apoptosis in target cells and have antimicrobial action. It is broadly antimicrobial, killing microbes that cause, for example, tuberculosis and malaria, and can destroy some tumors. A series of peptides generated from the amino acid sequence of granulysin are potential antibiotics. Granulysin increased the permeability of bacterial membranes, as judged by its ability to allow access of cytosolic ??-galactosidase to its impermeant substrate.Granulysin functions to create holes in the target cell membrane and destroy it. Granulysin is able to induce apoptosis in target cells and also has antimicrobial action.
mutable notes: Granulysin can be purified via nickel affinity chromatography.
mutable provenance: Granulysin is a member of the saposin-like protein (SAPLIP) family. The gene was synthesized at GENEART in pMA vector
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K258002_sequence/1

BBa_J52019
type: ComponentDefinition
persistent identity: BBa J52019
display id: BBa_J52019
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: dnTraf6
creator: Matej Skocaj
created: 2006-10-13T11:00:00Z
modified: 2015-08-31T03:54:04Z
discontinued: false
dominant: false
group u list: _80_
m user id: 0
owner id: 857
owning group id: 80
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: It is an imortant protein in signaling cascade through TLr receptors (MyD88 dependent pathway).
mutable notes: None.
mutable provenance: pDeNy
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: dnTraf6
sequence: https://synbiohub.org/public/igem/BBa_J52019_sequence/1

dnTraf6
type: SequenceAnnotation
persistent identity: annotation1903258
display id: annotation1903258
version: 1
top level: BBa_J52019
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_J52019/annotation1903258/range1903258/1

https://synbiohub.org/public/igem/BBa_J52019/annotation1903258/range1903258/1
type: Range
persistent identity: range1903258
display id: range1903258
version: 1
direction: direction/forward
top level: BBa_J52019
start: 1
end: 714
orientation: inline

dnTraf6
type: SequenceAnnotation
persistent identity: annotation1903257
display id: annotation1903257
version: 1
top level: BBa_J52019
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J52019/annotation1903257/range1903257/1

https://synbiohub.org/public/igem/BBa_J52019/annotation1903257/range1903257/1
type: Range
persistent identity: range1903257
display id: range1903257
version: 1
direction: direction/forward
top level: BBa_J52019
start: 1
end: 714
orientation: inline

BBa_K133016
type: ComponentDefinition
persistent identity: BBa K133016
display id: BBa_K133016
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CF
creator: Vid Kocar
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:09:55Z
discontinued: false
dominant: false
experience: experience/None
group u list: _231_
m user id: 0
owner id: 3351
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Whole chimeric protein
mutable notes: None
mutable provenance: Genomic DNA
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K133016_sequence/1

BBa_J52026
type: ComponentDefinition
persistent identity: BBa J52026
display id: BBa_J52026
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: dnMyD88-linker-GFP
creator: Jelka Pohar
created: 2006-10-14T11:00:00Z
modified: 2016-01-26T09:39:38Z
discontinued: false
dominant: false
group u list: _80_
m user id: 4206
owner id: 855
owning group id: 80
sample status: In stock
status: status/Available
bookmark: false
mutable description: dnMyD88 is a dominant negative form of protein MyD88 (an important protein in signaling cascade through TLR4 receptor). dnMyD88 is linked to GFP (acts as a reporter) through six aminoacid long protein linker.
mutable notes: dnMyD88-linker-GFP was made by using PCR overlap extension technique. This part is cloned in BioBrick vector pSB1AK3. Original sequence has PstI restriction site so we have to introduce a mutation without affect on amino acid sequence.
mutable provenance: dnMyD88- GFP- more to come
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: dnMyD88-linker-GFP
sequence annotation: GFP
sequence annotation: dnMyD88
sequence annotation: linker
sequence: https://synbiohub.org/public/igem/BBa_J52026_sequence/1

dnMyD88-linker-GFP
type: SequenceAnnotation
persistent identity: annotation1903011
display id: annotation1903011
version: 1
top level: BBa_J52026
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_J52026/annotation1903011/range1903011/1

https://synbiohub.org/public/igem/BBa_J52026/annotation1903011/range1903011/1
type: Range
persistent identity: range1903011
display id: range1903011
version: 1
direction: direction/forward
top level: BBa_J52026
start: 1
end: 1155
orientation: inline

GFP
type: SequenceAnnotation
persistent identity: annotation1903010
display id: annotation1903010
version: 1
top level: BBa_J52026
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J52026/annotation1903010/range1903010/1

https://synbiohub.org/public/igem/BBa_J52026/annotation1903010/range1903010/1
type: Range
persistent identity: range1903010
display id: range1903010
version: 1
direction: direction/forward
top level: BBa_J52026
start: 436
end: 1155
orientation: inline

dnMyD88
type: SequenceAnnotation
persistent identity: annotation1903008
display id: annotation1903008
version: 1
top level: BBa_J52026
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J52026/annotation1903008/range1903008/1

https://synbiohub.org/public/igem/BBa_J52026/annotation1903008/range1903008/1
type: Range
persistent identity: range1903008
display id: range1903008
version: 1
direction: direction/forward
top level: BBa_J52026
start: 1
end: 417
orientation: inline

linker
type: SequenceAnnotation
persistent identity: annotation1903009
display id: annotation1903009
version: 1
top level: BBa_J52026
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_J52026/annotation1903009/range1903009/1

https://synbiohub.org/public/igem/BBa_J52026/annotation1903009/range1903009/1
type: Range
persistent identity: range1903009
display id: range1903009
version: 1
direction: direction/forward
top level: BBa_J52026
start: 418
end: 435
orientation: inline

BBa_K133057
type: ComponentDefinition
persistent identity: BBa K133057
display id: BBa_K133057
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: HpaA
creator: Jerneja Mori
created: 2008-10-25T11:00:00Z
modified: 2015-05-08T01:09:56Z
discontinued: false
dominant: false
experience: experience/None
group u list: _231_
m user id: 0
owner id: 3350
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: A putative neuraminyllactose-binding hemagglutinin from Helicobacter pylori.
mutable notes: Cloned into pSB1AK3 vector.
mutable provenance: Genomic sequence from H. pylori.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K133057_sequence/1

BBa_K133046
type: ComponentDefinition
persistent identity: BBa K133046
display id: BBa_K133046
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CF215
creator: Ana Lasic
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:09:55Z
discontinued: false
dominant: false
experience: experience/None
group u list: _231_
m user id: 0
owner id: 3352
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This is a part that is composed of C-terminal end of chimeric flagellin (BBa_K133016). We used aminoacids from 215th aminoacid on. Chimeric flagellin is composed of C- and N-terminal part of E.coli flagellin and of variable region of H.pylori. The E.coli parts are responsible for binding and activation of cell membrane receptor TLR5 to initiate immune response, while H.pylori variable region was used to array the immune response against H.pylori. This part was used in combination with CF213 - chimeric flagellin (BBa_K133010). Between the CF213 and CF215 we have put H.pylori antigens in order to array the immune response against H.pylori.
mutable notes: It was important to split the chimeric flagellin in the variable region to assure that the antigen will be in the right place and not to interrupt the 3d structure of the protein.
mutable provenance: We used polimerase chain reaction from chimeric flagellin (BBa_K133016).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K133046_sequence/1

BBa_I712010
type: ComponentDefinition
persistent identity: BBa I712010
display id: BBa_I712010
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CD4 sequence without signal peptide
creator: peter cimermancic
created: 2007-10-22T11:00:00Z
modified: 2015-08-31T04:07:45Z
discontinued: false
dominant: false
group u list: _130_
m user id: 0
owner id: 1850
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: It`s a chemokine receptor protein.
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_I712010_sequence/1

BBa_J52035
type: ComponentDefinition
persistent identity: BBa J52035
display id: BBa_J52035
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: dnMyD88
creator: Ota Fekonja
created: 2006-10-14T11:00:00Z
modified: 2015-08-31T03:54:04Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _80_
m user id: 0
owner id: 799
owning group id: 80
sample status: In stock
status: status/Available
bookmark: true
mutable description: dnMyD88 is a dominant negative form of MyD88, which has an important role in TLR signalling pathway. dnMyD88 contains only TIR domain of MyD88.
mutable notes: /
mutable provenance: more to come...
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: dnMyD88
sequence: https://synbiohub.org/public/igem/BBa_J52035_sequence/1

dnMyD88
type: SequenceAnnotation
persistent identity: annotation2114917
display id: annotation2114917
version: 1
top level: BBa_J52035
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J52035/annotation2114917/range2114917/1

https://synbiohub.org/public/igem/BBa_J52035/annotation2114917/range2114917/1
type: Range
persistent identity: range2114917
display id: range2114917
version: 1
direction: direction/forward
top level: BBa_J52035
start: 1
end: 420
orientation: inline

BBa_J52011
type: ComponentDefinition
persistent identity: BBa J52011
display id: BBa_J52011
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: dnMyD88-linker-Rluc
creator: Monika Ciglic
created: 2006-10-13T11:00:00Z
modified: 2015-08-31T03:54:04Z
discontinued: false
dominant: false
group u list: _80_
m user id: 0
owner id: 800
owning group id: 80
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: dnMyD88 is a dominant negative form of protein MyD88 (an important protein in signaling cascade through TLR4 receptor), which was our main target for modifying the signaling path. dnMyD88 is linked to Rluc (which acts as a reporter) through six aminoacid long protein linker.
mutable notes: dnMyD88-linker-Rluc was made by using PCR ligation technique. This part is cloned in BioBrick vector pSB1AK3.
mutable provenance: -
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: dnMyD88-linker-Rluc
sequence annotation: dnMyD88
sequence annotation: linker
sequence annotation: Rluc
sequence: https://synbiohub.org/public/igem/BBa_J52011_sequence/1

dnMyD88-linker-Rluc
type: SequenceAnnotation
persistent identity: annotation1902846
display id: annotation1902846
version: 1
top level: BBa_J52011
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_J52011/annotation1902846/range1902846/1

https://synbiohub.org/public/igem/BBa_J52011/annotation1902846/range1902846/1
type: Range
persistent identity: range1902846
display id: range1902846
version: 1
direction: direction/forward
top level: BBa_J52011
start: 1
end: 1371
orientation: inline

dnMyD88
type: SequenceAnnotation
persistent identity: annotation1902834
display id: annotation1902834
version: 1
top level: BBa_J52011
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J52011/annotation1902834/range1902834/1

https://synbiohub.org/public/igem/BBa_J52011/annotation1902834/range1902834/1
type: Range
persistent identity: range1902834
display id: range1902834
version: 1
direction: direction/forward
top level: BBa_J52011
start: 1
end: 417
orientation: inline

linker
type: SequenceAnnotation
persistent identity: annotation1902835
display id: annotation1902835
version: 1
top level: BBa_J52011
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_J52011/annotation1902835/range1902835/1

https://synbiohub.org/public/igem/BBa_J52011/annotation1902835/range1902835/1
type: Range
persistent identity: range1902835
display id: range1902835
version: 1
direction: direction/forward
top level: BBa_J52011
start: 418
end: 435
orientation: inline

Rluc
type: SequenceAnnotation
persistent identity: annotation1902836
display id: annotation1902836
version: 1
top level: BBa_J52011
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_J52011/annotation1902836/range1902836/1

https://synbiohub.org/public/igem/BBa_J52011/annotation1902836/range1902836/1
type: Range
persistent identity: range1902836
display id: range1902836
version: 1
direction: direction/forward
top level: BBa_J52011
start: 436
end: 1371
orientation: inline

BBa_I712017
type: ComponentDefinition
persistent identity: BBa I712017
display id: BBa_I712017
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Chemokine (CXC motif) receptor 4, fused to N-terminal half of ubiquitin.
creator: Anja Korenčič
created: 2007-10-20T11:00:00Z
modified: 2015-08-31T04:07:46Z
discontinued: false
dominant: false
group u list: _130_
m user id: 0
owner id: 1846
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Chemokine (CXC motif) receptor 4, fused to N-terminal half of ubiquitin.
mutable notes: CXCR4 and NUb fragments were fused with PCR overlap extension method without any linker added. The part is cloned into BBa_J52017 (a vector with eukaryontic terminator).
mutable provenance: CXCR4 gene obtained from Origene, NUb gene obtained from Dualsystems.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: CXCR4
sequence annotation: NUb
sequence: https://synbiohub.org/public/igem/BBa_I712017_sequence/1

CXCR4
type: SequenceAnnotation
persistent identity: annotation1951574
display id: annotation1951574
version: 1
top level: BBa_I712017
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_I712017/annotation1951574/range1951574/1

https://synbiohub.org/public/igem/BBa_I712017/annotation1951574/range1951574/1
type: Range
persistent identity: range1951574
display id: range1951574
version: 1
direction: direction/forward
top level: BBa_I712017
start: 1
end: 1056
orientation: inline

NUb
type: SequenceAnnotation
persistent identity: annotation1951575
display id: annotation1951575
version: 1
top level: BBa_I712017
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_I712017/annotation1951575/range1951575/1

https://synbiohub.org/public/igem/BBa_I712017/annotation1951575/range1951575/1
type: Range
persistent identity: range1951575
display id: range1951575
version: 1
direction: direction/forward
top level: BBa_I712017
start: 1057
end: 1191
orientation: inline

BBa_K133010
type: ComponentDefinition
persistent identity: BBa K133010
display id: BBa_K133010
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CF213
creator: Vid Kocar
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:09:55Z
discontinued: false
dominant: false
experience: experience/None
group u list: _231_
m user id: 0
owner id: 3351
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Chimeric flagellin
mutable notes: None
mutable provenance: Genomic DNA
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K133010_sequence/1

BBa_I712012
type: ComponentDefinition
persistent identity: BBa I712012
display id: BBa_I712012
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TIR domain of TLR3
creator: Andrej Ondracka
created: 2007-10-20T11:00:00Z
modified: 2015-08-31T04:07:45Z
discontinued: false
dominant: false
group u list: _130_
m user id: 0
owner id: 1896
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: Toll-like receptor 3 cytoplasmic domain.
mutable notes: Cloned into pSBAK3 vector with terminator. Start codon is NOT included.
mutable provenance: amplified from TLR3 gene, obtained from
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: TIR domain
sequence: https://synbiohub.org/public/igem/BBa_I712012_sequence/1

TIR domain
type: SequenceAnnotation
persistent identity: annotation1951817
display id: annotation1951817
version: 1
top level: BBa_I712012
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_I712012/annotation1951817/range1951817/1

https://synbiohub.org/public/igem/BBa_I712012/annotation1951817/range1951817/1
type: Range
persistent identity: range1951817
display id: range1951817
version: 1
direction: direction/forward
top level: BBa_I712012
start: 1
end: 456
orientation: inline

scFv
type: ComponentDefinition
persistent identity: BBa K1933002
display id: BBa_K1933002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: anti-Norovirus GII.4 scFv
creator: Tomoki Uchino
created: 2016-10-09T11:00:00Z
modified: 2016-10-18T10:28:59Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2400_
m user id: 23701
owner id: 29998
owning group id: 9
status: status/Planning
bookmark: true
mutable description: This part codes anti-Norovirus GII.4 scFv (single-chain variable fragment) which derives from human 12A2 antibody. This scFv is suggested to prevent binding of human norovirus to Hist-Blood Group Antigens. [1] This coding sequence was fused to INPNC-His (part number: ) or BclA-His (part number: ) in our project. For more information, please visit our Wiki page.
mutable notes: silent mutation was made to remove PstI: G255C
mutable provenance: We have been given given the scFv-encoding plasmid (NoV GII.4 strain-specific antibody 12A2 (vector: pSCCA5-E8d)) from Dr. Kyoko Moriguchi of the Fujita Health University Department of Virology Parasitology.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: VH
sequence annotation: linker
sequence annotation: G255C
sequence annotation: start codon
sequence annotation: VL
sequence annotation: stop codon
sequence: https://synbiohub.org/public/igem/BBa_K1933002_sequence/1

VH
type: SequenceAnnotation
persistent identity: annotation2490183
display id: annotation2490183
version: 1
top level: scFv
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1933002/annotation2490183/range2490183/1

https://synbiohub.org/public/igem/BBa_K1933002/annotation2490183/range2490183/1
type: Range
persistent identity: range2490183
display id: range2490183
version: 1
direction: direction/forward
top level: scFv
start: 4
end: 392
orientation: inline

linker
type: SequenceAnnotation
persistent identity: annotation2490184
display id: annotation2490184
version: 1
top level: scFv
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1933002/annotation2490184/range2490184/1

https://synbiohub.org/public/igem/BBa_K1933002/annotation2490184/range2490184/1
type: Range
persistent identity: range2490184
display id: range2490184
version: 1
direction: direction/forward
top level: scFv
start: 393
end: 438
orientation: inline

G255C
type: SequenceAnnotation
persistent identity: annotation2490197
display id: annotation2490197
version: 1
top level: scFv
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_K1933002/annotation2490197/range2490197/1

https://synbiohub.org/public/igem/BBa_K1933002/annotation2490197/range2490197/1
type: Range
persistent identity: range2490197
display id: range2490197
version: 1
direction: direction/forward
top level: scFv
start: 255
end: 255
orientation: inline

start codon
type: SequenceAnnotation
persistent identity: annotation2490182
display id: annotation2490182
version: 1
top level: scFv
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1933002/annotation2490182/range2490182/1

https://synbiohub.org/public/igem/BBa_K1933002/annotation2490182/range2490182/1
type: Range
persistent identity: range2490182
display id: range2490182
version: 1
direction: direction/forward
top level: scFv
start: 1
end: 3
orientation: inline

VL
type: SequenceAnnotation
persistent identity: annotation2490185
display id: annotation2490185
version: 1
top level: scFv
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1933002/annotation2490185/range2490185/1

https://synbiohub.org/public/igem/BBa_K1933002/annotation2490185/range2490185/1
type: Range
persistent identity: range2490185
display id: range2490185
version: 1
direction: direction/forward
top level: scFv
start: 439
end: 777
orientation: inline

stop codon
type: SequenceAnnotation
persistent identity: annotation2490186
display id: annotation2490186
version: 1
top level: scFv
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1933002/annotation2490186/range2490186/1

https://synbiohub.org/public/igem/BBa_K1933002/annotation2490186/range2490186/1
type: Range
persistent identity: range2490186
display id: range2490186
version: 1
direction: direction/forward
top level: scFv
start: 778
end: 780
orientation: inline

BBa_J52021
type: ComponentDefinition
persistent identity: BBa J52021
display id: BBa_J52021
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: dnTraf6-linker-GFP
creator: Matej Skocaj
created: 2006-10-13T11:00:00Z
modified: 2016-01-26T09:38:59Z
discontinued: false
dominant: false
experience: experience/None
group u list: _80_
m user id: 0
m user id: 4206
owner id: 857
owning group id: 80
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Part is composed of TIR domain of Traf6 and GFP linked with 6 aminoacids long linker. GFP serves as reporter. For usage in eukaryotes.
mutable notes: None.
mutable provenance: dnTraf6- pDeNy GFP-
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: GFP
sequence annotation: linker
sequence annotation: dnTraf6-linker-GFP
sequence annotation: dnTraf6
sequence: https://synbiohub.org/public/igem/BBa_J52021_sequence/1

GFP
type: SequenceAnnotation
persistent identity: annotation1903261
display id: annotation1903261
version: 1
top level: BBa_J52021
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J52021/annotation1903261/range1903261/1

https://synbiohub.org/public/igem/BBa_J52021/annotation1903261/range1903261/1
type: Range
persistent identity: range1903261
display id: range1903261
version: 1
direction: direction/forward
top level: BBa_J52021
start: 733
end: 1446
orientation: inline

linker
type: SequenceAnnotation
persistent identity: annotation1903260
display id: annotation1903260
version: 1
top level: BBa_J52021
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_J52021/annotation1903260/range1903260/1

https://synbiohub.org/public/igem/BBa_J52021/annotation1903260/range1903260/1
type: Range
persistent identity: range1903260
display id: range1903260
version: 1
direction: direction/forward
top level: BBa_J52021
start: 715
end: 732
orientation: inline

dnTraf6-linker-GFP
type: SequenceAnnotation
persistent identity: annotation1903262
display id: annotation1903262
version: 1
top level: BBa_J52021
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_J52021/annotation1903262/range1903262/1

https://synbiohub.org/public/igem/BBa_J52021/annotation1903262/range1903262/1
type: Range
persistent identity: range1903262
display id: range1903262
version: 1
direction: direction/forward
top level: BBa_J52021
start: 1
end: 1446
orientation: inline

dnTraf6
type: SequenceAnnotation
persistent identity: annotation1903259
display id: annotation1903259
version: 1
top level: BBa_J52021
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J52021/annotation1903259/range1903259/1

https://synbiohub.org/public/igem/BBa_J52021/annotation1903259/range1903259/1
type: Range
persistent identity: range1903259
display id: range1903259
version: 1
direction: direction/forward
top level: BBa_J52021
start: 1
end: 714
orientation: inline

BBa_I712024
type: ComponentDefinition
persistent identity: BBa I712024
display id: BBa_I712024
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CD4
creator: Katja Kolar
created: 2007-10-22T11:00:00Z
modified: 2015-08-31T04:07:46Z
discontinued: false
dominant: false
group u list: _130_
m user id: 0
owner id: 1883
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Sequence for chemokine receptor CD4, including signal peptide.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_I712024_sequence/1

BBa_I712002
type: ComponentDefinition
persistent identity: BBa I712002
display id: BBa_I712002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CCR5
creator: Andrej Ondracka
created: 2007-10-20T11:00:00Z
modified: 2015-08-31T04:07:45Z
discontinued: false
dominant: false
group u list: _130_
m user id: 0
owner id: 1896
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Chemokine (C-C motif) receptor 5, a HIV-1 co-receptor.
mutable notes: The part is cloned into BBa_J52017, a vector with eukaryontic terminator. PstI and EcoRI restriction sites inside CCR5 gene were mutated.
mutable provenance: CCR5 gene was obtained from Origene.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: CCR5
sequence: https://synbiohub.org/public/igem/BBa_I712002_sequence/1

CCR5
type: SequenceAnnotation
persistent identity: annotation1951768
display id: annotation1951768
version: 1
top level: BBa_I712002
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_I712002/annotation1951768/range1951768/1

https://synbiohub.org/public/igem/BBa_I712002/annotation1951768/range1951768/1
type: Range
persistent identity: range1951768
display id: range1951768
version: 1
direction: direction/forward
top level: BBa_I712002
start: 1
end: 1059
orientation: inline