//function/recombination/fim
type: Collection
persistent identity: function recombination fim
display id: function_recombination_fim
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
owned by: james
owned by: myers
member: BBa_K137060
member: BBa_K1632007
member: IRR
member: BBa_K137058
member: BBa_K137007
member: BBa_K137059
member: BBa_K137057
member: BBa_K1632000
member: fimE IRL
member: BBa_K137062
member: BBa_K137061

BBa_K137060
type: ComponentDefinition
persistent identity: BBa K137060
display id: BBa_K137060
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: GFP fimE switch with 450 bp tetR promoter
creator: Allen Lin
created: 2008-07-30T11:00:00Z
modified: 2015-05-08T01:10:09Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _187_
m user id: 0
owner id: 3112
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: A promoter facing upstream is flanked by two FimE binding sites. GFP is downstream to this fimE switch. Active FimE will flip the promoter such that GFP is expresssed.
mutable notes: None.
mutable provenance: Previously constructed Biobrick parts.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_E0040
component: BBa_K137010
component: BBa_K137008
component: BBa_B0010
component: BBa_K137049
component: BBa_B0034
component: BBa_B0012
sequence annotation: BBa_B0010
sequence annotation: BBa_B0034
sequence annotation: BBa_K137010
sequence annotation: BBa_B0012
sequence annotation: BBa_E0040
sequence annotation: BBa_K137008
sequence annotation: BBa_K137049
sequence: https://synbiohub.org/public/igem/BBa_K137060_sequence/1

BBa_E0040
type: Component
persistent identity: component1969335
display id: component1969335
version: 1
top level: BBa_K137060
access: public
definition: https://synbiohub.org/public/igem/BBa_E0040/1

BBa_K137010
type: Component
persistent identity: component1969326
display id: component1969326
version: 1
top level: BBa_K137060
access: public
definition: fimE IRL

BBa_K137008
type: Component
persistent identity: component1969331
display id: component1969331
version: 1
top level: BBa_K137060
access: public
definition: IRR

BBa_B0010
type: Component
persistent identity: component1969336
display id: component1969336
version: 1
top level: BBa_K137060
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_K137049
type: Component
persistent identity: component1969328
display id: component1969328
version: 1
top level: BBa_K137060
access: public
definition: https://synbiohub.org/public/igem/BBa_K137049/1

BBa_B0034
type: Component
persistent identity: component1969333
display id: component1969333
version: 1
top level: BBa_K137060
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0012
type: Component
persistent identity: component1969338
display id: component1969338
version: 1
top level: BBa_K137060
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1969336
display id: annotation1969336
version: 1
top level: BBa_K137060
location: https://synbiohub.org/public/igem/BBa_K137060/annotation1969336/range1969336/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K137060/annotation1969336/range1969336/1
type: Range
persistent identity: range1969336
display id: range1969336
version: 1
direction: direction/forward
top level: BBa_K137060
start: 1291
end: 1370
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1969333
display id: annotation1969333
version: 1
top level: BBa_K137060
location: https://synbiohub.org/public/igem/BBa_K137060/annotation1969333/range1969333/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K137060/annotation1969333/range1969333/1
type: Range
persistent identity: range1969333
display id: range1969333
version: 1
direction: direction/reverse
top level: BBa_K137060
start: 545
end: 556
orientation: inline

BBa_K137010
type: SequenceAnnotation
persistent identity: annotation1969326
display id: annotation1969326
version: 1
top level: BBa_K137060
location: https://synbiohub.org/public/igem/BBa_K137060/annotation1969326/range1969326/1
component: BBa_K137010

https://synbiohub.org/public/igem/BBa_K137060/annotation1969326/range1969326/1
type: Range
persistent identity: range1969326
display id: range1969326
version: 1
direction: direction/reverse
top level: BBa_K137060
start: 1
end: 35
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1969338
display id: annotation1969338
version: 1
top level: BBa_K137060
location: https://synbiohub.org/public/igem/BBa_K137060/annotation1969338/range1969338/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K137060/annotation1969338/range1969338/1
type: Range
persistent identity: range1969338
display id: range1969338
version: 1
direction: direction/forward
top level: BBa_K137060
start: 1379
end: 1419
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation1969335
display id: annotation1969335
version: 1
top level: BBa_K137060
location: https://synbiohub.org/public/igem/BBa_K137060/annotation1969335/range1969335/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_K137060/annotation1969335/range1969335/1
type: Range
persistent identity: range1969335
display id: range1969335
version: 1
direction: direction/reverse
top level: BBa_K137060
start: 563
end: 1282
orientation: inline

BBa_K137008
type: SequenceAnnotation
persistent identity: annotation1969331
display id: annotation1969331
version: 1
top level: BBa_K137060
location: https://synbiohub.org/public/igem/BBa_K137060/annotation1969331/range1969331/1
component: BBa_K137008

https://synbiohub.org/public/igem/BBa_K137060/annotation1969331/range1969331/1
type: Range
persistent identity: range1969331
display id: range1969331
version: 1
direction: direction/reverse
top level: BBa_K137060
start: 502
end: 536
orientation: inline

BBa_K137049
type: SequenceAnnotation
persistent identity: annotation1969328
display id: annotation1969328
version: 1
top level: BBa_K137060
location: https://synbiohub.org/public/igem/BBa_K137060/annotation1969328/range1969328/1
component: BBa_K137049

https://synbiohub.org/public/igem/BBa_K137060/annotation1969328/range1969328/1
type: Range
persistent identity: range1969328
display id: range1969328
version: 1
direction: direction/reverse
top level: BBa_K137060
start: 44
end: 493
orientation: inline

BBa_K137061
type: ComponentDefinition
persistent identity: BBa K137061
display id: BBa_K137061
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: GFP fimE switch with 650 bp tetR promoter
creator: Allen Lin
created: 2008-07-30T11:00:00Z
modified: 2015-05-08T01:10:09Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _187_
m user id: 0
owner id: 3112
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: A promoter facing upstream is flanked by two FimE binding sites. GFP is downstream to this fimE switch. Active FimE will flip the promoter such that GFP is expresssed.
mutable notes: None.
mutable provenance: Previously constructed Biobrick parts.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_B0012
component: BBa_K137010
component: BBa_K137050
component: BBa_B0010
component: BBa_B0034
component: BBa_K137008
component: BBa_E0040
sequence annotation: BBa_E0040
sequence annotation: BBa_B0034
sequence annotation: BBa_K137010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_K137050
sequence annotation: BBa_K137008
sequence: https://synbiohub.org/public/igem/BBa_K137061_sequence/1

BBa_B0012
type: Component
persistent identity: component1969356
display id: component1969356
version: 1
top level: BBa_K137061
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_K137010
type: Component
persistent identity: component1969344
display id: component1969344
version: 1
top level: BBa_K137061
access: public
definition: fimE IRL

BBa_K137050
type: Component
persistent identity: component1969346
display id: component1969346
version: 1
top level: BBa_K137061
access: public
definition: https://synbiohub.org/public/igem/BBa_K137050/1

BBa_B0010
type: Component
persistent identity: component1969354
display id: component1969354
version: 1
top level: BBa_K137061
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0034
type: Component
persistent identity: component1969351
display id: component1969351
version: 1
top level: BBa_K137061
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K137008
type: Component
persistent identity: component1969349
display id: component1969349
version: 1
top level: BBa_K137061
access: public
definition: IRR

BBa_E0040
type: Component
persistent identity: component1969353
display id: component1969353
version: 1
top level: BBa_K137061
access: public
definition: https://synbiohub.org/public/igem/BBa_E0040/1

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation1969353
display id: annotation1969353
version: 1
top level: BBa_K137061
location: https://synbiohub.org/public/igem/BBa_K137061/annotation1969353/range1969353/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_K137061/annotation1969353/range1969353/1
type: Range
persistent identity: range1969353
display id: range1969353
version: 1
direction: direction/reverse
top level: BBa_K137061
start: 763
end: 1482
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1969351
display id: annotation1969351
version: 1
top level: BBa_K137061
location: https://synbiohub.org/public/igem/BBa_K137061/annotation1969351/range1969351/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K137061/annotation1969351/range1969351/1
type: Range
persistent identity: range1969351
display id: range1969351
version: 1
direction: direction/reverse
top level: BBa_K137061
start: 745
end: 756
orientation: inline

BBa_K137010
type: SequenceAnnotation
persistent identity: annotation1969344
display id: annotation1969344
version: 1
top level: BBa_K137061
location: https://synbiohub.org/public/igem/BBa_K137061/annotation1969344/range1969344/1
component: BBa_K137010

https://synbiohub.org/public/igem/BBa_K137061/annotation1969344/range1969344/1
type: Range
persistent identity: range1969344
display id: range1969344
version: 1
direction: direction/reverse
top level: BBa_K137061
start: 1
end: 35
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1969354
display id: annotation1969354
version: 1
top level: BBa_K137061
location: https://synbiohub.org/public/igem/BBa_K137061/annotation1969354/range1969354/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K137061/annotation1969354/range1969354/1
type: Range
persistent identity: range1969354
display id: range1969354
version: 1
direction: direction/forward
top level: BBa_K137061
start: 1491
end: 1570
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1969356
display id: annotation1969356
version: 1
top level: BBa_K137061
location: https://synbiohub.org/public/igem/BBa_K137061/annotation1969356/range1969356/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K137061/annotation1969356/range1969356/1
type: Range
persistent identity: range1969356
display id: range1969356
version: 1
direction: direction/forward
top level: BBa_K137061
start: 1579
end: 1619
orientation: inline

BBa_K137050
type: SequenceAnnotation
persistent identity: annotation1969346
display id: annotation1969346
version: 1
top level: BBa_K137061
location: https://synbiohub.org/public/igem/BBa_K137061/annotation1969346/range1969346/1
component: BBa_K137050

https://synbiohub.org/public/igem/BBa_K137061/annotation1969346/range1969346/1
type: Range
persistent identity: range1969346
display id: range1969346
version: 1
direction: direction/reverse
top level: BBa_K137061
start: 44
end: 693
orientation: inline

BBa_K137008
type: SequenceAnnotation
persistent identity: annotation1969349
display id: annotation1969349
version: 1
top level: BBa_K137061
location: https://synbiohub.org/public/igem/BBa_K137061/annotation1969349/range1969349/1
component: BBa_K137008

https://synbiohub.org/public/igem/BBa_K137061/annotation1969349/range1969349/1
type: Range
persistent identity: range1969349
display id: range1969349
version: 1
direction: direction/reverse
top level: BBa_K137061
start: 702
end: 736
orientation: inline

BBa_K137062
type: ComponentDefinition
persistent identity: BBa K137062
display id: BBa_K137062
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: GFP fimE switch with 850 bp tetR promoter
creator: Allen Lin
created: 2008-07-30T11:00:00Z
modified: 2015-05-08T01:10:09Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _187_
m user id: 0
owner id: 3112
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: A promoter facing upstream is flanked by two FimE binding sites. GFP is downstream to this fimE switch. Active FimE will flip the promoter such that GFP is expresssed.
mutable notes: None.
mutable provenance: Previously constructed Biobrick parts.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_K137051
component: BBa_E0040
component: BBa_K137008
component: BBa_B0010
component: BBa_B0034
component: BBa_B0012
component: BBa_K137010
sequence annotation: BBa_K137010
sequence annotation: BBa_B0012
sequence annotation: BBa_K137008
sequence annotation: BBa_B0034
sequence annotation: BBa_E0040
sequence annotation: BBa_B0010
sequence annotation: BBa_K137051
sequence: https://synbiohub.org/public/igem/BBa_K137062_sequence/1

BBa_K137051
type: Component
persistent identity: component1969364
display id: component1969364
version: 1
top level: BBa_K137062
access: public
definition: https://synbiohub.org/public/igem/BBa_K137051/1

BBa_E0040
type: Component
persistent identity: component1969371
display id: component1969371
version: 1
top level: BBa_K137062
access: public
definition: https://synbiohub.org/public/igem/BBa_E0040/1

BBa_K137008
type: Component
persistent identity: component1969367
display id: component1969367
version: 1
top level: BBa_K137062
access: public
definition: IRR

BBa_B0010
type: Component
persistent identity: component1969372
display id: component1969372
version: 1
top level: BBa_K137062
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0034
type: Component
persistent identity: component1969369
display id: component1969369
version: 1
top level: BBa_K137062
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0012
type: Component
persistent identity: component1969374
display id: component1969374
version: 1
top level: BBa_K137062
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_K137010
type: Component
persistent identity: component1969362
display id: component1969362
version: 1
top level: BBa_K137062
access: public
definition: fimE IRL

BBa_K137010
type: SequenceAnnotation
persistent identity: annotation1969362
display id: annotation1969362
version: 1
top level: BBa_K137062
location: https://synbiohub.org/public/igem/BBa_K137062/annotation1969362/range1969362/1
component: BBa_K137010

https://synbiohub.org/public/igem/BBa_K137062/annotation1969362/range1969362/1
type: Range
persistent identity: range1969362
display id: range1969362
version: 1
direction: direction/reverse
top level: BBa_K137062
start: 1
end: 35
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1969374
display id: annotation1969374
version: 1
top level: BBa_K137062
location: https://synbiohub.org/public/igem/BBa_K137062/annotation1969374/range1969374/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K137062/annotation1969374/range1969374/1
type: Range
persistent identity: range1969374
display id: range1969374
version: 1
direction: direction/forward
top level: BBa_K137062
start: 1779
end: 1819
orientation: inline

BBa_K137008
type: SequenceAnnotation
persistent identity: annotation1969367
display id: annotation1969367
version: 1
top level: BBa_K137062
location: https://synbiohub.org/public/igem/BBa_K137062/annotation1969367/range1969367/1
component: BBa_K137008

https://synbiohub.org/public/igem/BBa_K137062/annotation1969367/range1969367/1
type: Range
persistent identity: range1969367
display id: range1969367
version: 1
direction: direction/reverse
top level: BBa_K137062
start: 902
end: 936
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1969369
display id: annotation1969369
version: 1
top level: BBa_K137062
location: https://synbiohub.org/public/igem/BBa_K137062/annotation1969369/range1969369/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K137062/annotation1969369/range1969369/1
type: Range
persistent identity: range1969369
display id: range1969369
version: 1
direction: direction/reverse
top level: BBa_K137062
start: 945
end: 956
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation1969371
display id: annotation1969371
version: 1
top level: BBa_K137062
location: https://synbiohub.org/public/igem/BBa_K137062/annotation1969371/range1969371/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_K137062/annotation1969371/range1969371/1
type: Range
persistent identity: range1969371
display id: range1969371
version: 1
direction: direction/reverse
top level: BBa_K137062
start: 963
end: 1682
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1969372
display id: annotation1969372
version: 1
top level: BBa_K137062
location: https://synbiohub.org/public/igem/BBa_K137062/annotation1969372/range1969372/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K137062/annotation1969372/range1969372/1
type: Range
persistent identity: range1969372
display id: range1969372
version: 1
direction: direction/forward
top level: BBa_K137062
start: 1691
end: 1770
orientation: inline

BBa_K137051
type: SequenceAnnotation
persistent identity: annotation1969364
display id: annotation1969364
version: 1
top level: BBa_K137062
location: https://synbiohub.org/public/igem/BBa_K137062/annotation1969364/range1969364/1
component: BBa_K137051

https://synbiohub.org/public/igem/BBa_K137062/annotation1969364/range1969364/1
type: Range
persistent identity: range1969364
display id: range1969364
version: 1
direction: direction/reverse
top level: BBa_K137062
start: 44
end: 893
orientation: inline

BBa_K137007
type: ComponentDefinition
persistent identity: BBa K137007
display id: BBa_K137007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: fimE
creator: Allen Lin
created: 2008-06-19T11:00:00Z
modified: 2015-05-08T01:10:08Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _187_
m user id: 0
owner id: 3112
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: fimE gene
mutable notes: none
mutable provenance: pFIP plasmid
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K137007_sequence/1

fimE IRL
type: ComponentDefinition
persistent identity: BBa K137010
display id: BBa_K137010
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: fimE IRL
creator: Allen Lin
created: 2008-06-19T11:00:00Z
modified: 2015-05-08T01:10:08Z
discontinued: false
dominant: false
experience: experience/Issues
group u list: _187_
m user id: 0
owner id: 3112
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: fimE inverted repeat left recombination site
mutable notes: none
mutable provenance: pFIP plasmid
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: IRL out
sequence annotation: IRL in
sequence: https://synbiohub.org/public/igem/BBa_K137010_sequence/1

IRL out
type: SequenceAnnotation
persistent identity: annotation1963844
display id: annotation1963844
version: 1
top level: fimE IRL
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K137010/annotation1963844/range1963844/1

https://synbiohub.org/public/igem/BBa_K137010/annotation1963844/range1963844/1
type: Range
persistent identity: range1963844
display id: range1963844
version: 1
direction: direction/forward
top level: fimE IRL
start: 19
end: 35
orientation: inline

IRL in
type: SequenceAnnotation
persistent identity: annotation1963843
display id: annotation1963843
version: 1
top level: fimE IRL
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K137010/annotation1963843/range1963843/1

https://synbiohub.org/public/igem/BBa_K137010/annotation1963843/range1963843/1
type: Range
persistent identity: range1963843
display id: range1963843
version: 1
direction: direction/forward
top level: fimE IRL
start: 1
end: 17
orientation: inline

BBa_K137058
type: ComponentDefinition
persistent identity: BBa K137058
display id: BBa_K137058
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: GFP fimE switch with 250 bp tetR promoter
creator: Allen Lin
created: 2008-07-30T11:00:00Z
modified: 2015-05-08T01:10:09Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _187_
m user id: 0
owner id: 3112
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: A promoter facing upstream is flanked by two FimE binding sites. GFP is downstream to this fimE switch. Active FimE will flip the promoter such that GFP is expresssed.
mutable notes: None.
mutable provenance: Previously constructed Biobrick parts.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_K137008
component: BBa_B0012
component: BBa_K137010
component: BBa_K137047
component: BBa_B0034
component: BBa_E0040
component: BBa_B0010
sequence annotation: BBa_K137010
sequence annotation: BBa_K137008
sequence annotation: BBa_B0010
sequence annotation: BBa_E0040
sequence annotation: BBa_B0034
sequence annotation: BBa_K137047
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_K137058_sequence/1

BBa_K137008
type: Component
persistent identity: component1969295
display id: component1969295
version: 1
top level: BBa_K137058
access: public
definition: IRR

BBa_B0012
type: Component
persistent identity: component1969302
display id: component1969302
version: 1
top level: BBa_K137058
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_K137010
type: Component
persistent identity: component1969290
display id: component1969290
version: 1
top level: BBa_K137058
access: public
definition: fimE IRL

BBa_K137047
type: Component
persistent identity: component1969292
display id: component1969292
version: 1
top level: BBa_K137058
access: public
definition: https://synbiohub.org/public/igem/BBa_K137047/1

BBa_B0034
type: Component
persistent identity: component1969297
display id: component1969297
version: 1
top level: BBa_K137058
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_E0040
type: Component
persistent identity: component1969299
display id: component1969299
version: 1
top level: BBa_K137058
access: public
definition: https://synbiohub.org/public/igem/BBa_E0040/1

BBa_B0010
type: Component
persistent identity: component1969300
display id: component1969300
version: 1
top level: BBa_K137058
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_K137010
type: SequenceAnnotation
persistent identity: annotation1969290
display id: annotation1969290
version: 1
top level: BBa_K137058
location: https://synbiohub.org/public/igem/BBa_K137058/annotation1969290/range1969290/1
component: BBa_K137010

https://synbiohub.org/public/igem/BBa_K137058/annotation1969290/range1969290/1
type: Range
persistent identity: range1969290
display id: range1969290
version: 1
direction: direction/reverse
top level: BBa_K137058
start: 1
end: 35
orientation: inline

BBa_K137008
type: SequenceAnnotation
persistent identity: annotation1969295
display id: annotation1969295
version: 1
top level: BBa_K137058
location: https://synbiohub.org/public/igem/BBa_K137058/annotation1969295/range1969295/1
component: BBa_K137008

https://synbiohub.org/public/igem/BBa_K137058/annotation1969295/range1969295/1
type: Range
persistent identity: range1969295
display id: range1969295
version: 1
direction: direction/reverse
top level: BBa_K137058
start: 302
end: 336
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1969300
display id: annotation1969300
version: 1
top level: BBa_K137058
location: https://synbiohub.org/public/igem/BBa_K137058/annotation1969300/range1969300/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K137058/annotation1969300/range1969300/1
type: Range
persistent identity: range1969300
display id: range1969300
version: 1
direction: direction/forward
top level: BBa_K137058
start: 1091
end: 1170
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation1969299
display id: annotation1969299
version: 1
top level: BBa_K137058
location: https://synbiohub.org/public/igem/BBa_K137058/annotation1969299/range1969299/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_K137058/annotation1969299/range1969299/1
type: Range
persistent identity: range1969299
display id: range1969299
version: 1
direction: direction/reverse
top level: BBa_K137058
start: 363
end: 1082
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1969297
display id: annotation1969297
version: 1
top level: BBa_K137058
location: https://synbiohub.org/public/igem/BBa_K137058/annotation1969297/range1969297/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K137058/annotation1969297/range1969297/1
type: Range
persistent identity: range1969297
display id: range1969297
version: 1
direction: direction/reverse
top level: BBa_K137058
start: 345
end: 356
orientation: inline

BBa_K137047
type: SequenceAnnotation
persistent identity: annotation1969292
display id: annotation1969292
version: 1
top level: BBa_K137058
location: https://synbiohub.org/public/igem/BBa_K137058/annotation1969292/range1969292/1
component: BBa_K137047

https://synbiohub.org/public/igem/BBa_K137058/annotation1969292/range1969292/1
type: Range
persistent identity: range1969292
display id: range1969292
version: 1
direction: direction/reverse
top level: BBa_K137058
start: 44
end: 293
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1969302
display id: annotation1969302
version: 1
top level: BBa_K137058
location: https://synbiohub.org/public/igem/BBa_K137058/annotation1969302/range1969302/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K137058/annotation1969302/range1969302/1
type: Range
persistent identity: range1969302
display id: range1969302
version: 1
direction: direction/forward
top level: BBa_K137058
start: 1179
end: 1219
orientation: inline

BBa_K137059
type: ComponentDefinition
persistent identity: BBa K137059
display id: BBa_K137059
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: GFP fimE switch with 350 bp tetR promoter
creator: Allen Lin
created: 2008-07-30T11:00:00Z
modified: 2015-05-08T01:10:09Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _187_
m user id: 0
owner id: 3112
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: A promoter facing upstream is flanked by two FimE binding sites. GFP is downstream to this fimE switch. Active FimE will flip the promoter such that GFP is expresssed.
mutable notes: None.
mutable provenance: Previously constructed Biobrick parts.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_K137048
component: BBa_B0034
component: BBa_E0040
component: BBa_K137008
component: BBa_K137010
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_B0034
sequence annotation: BBa_K137008
sequence annotation: BBa_E0040
sequence annotation: BBa_K137048
sequence annotation: BBa_B0010
sequence annotation: BBa_K137010
sequence: https://synbiohub.org/public/igem/BBa_K137059_sequence/1

BBa_K137048
type: Component
persistent identity: component1969310
display id: component1969310
version: 1
top level: BBa_K137059
access: public
definition: https://synbiohub.org/public/igem/BBa_K137048/1

BBa_B0034
type: Component
persistent identity: component1969315
display id: component1969315
version: 1
top level: BBa_K137059
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_E0040
type: Component
persistent identity: component1969317
display id: component1969317
version: 1
top level: BBa_K137059
access: public
definition: https://synbiohub.org/public/igem/BBa_E0040/1

BBa_K137008
type: Component
persistent identity: component1969313
display id: component1969313
version: 1
top level: BBa_K137059
access: public
definition: IRR

BBa_K137010
type: Component
persistent identity: component1969308
display id: component1969308
version: 1
top level: BBa_K137059
access: public
definition: fimE IRL

BBa_B0012
type: Component
persistent identity: component1969320
display id: component1969320
version: 1
top level: BBa_K137059
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0010
type: Component
persistent identity: component1969318
display id: component1969318
version: 1
top level: BBa_K137059
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1969320
display id: annotation1969320
version: 1
top level: BBa_K137059
location: https://synbiohub.org/public/igem/BBa_K137059/annotation1969320/range1969320/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K137059/annotation1969320/range1969320/1
type: Range
persistent identity: range1969320
display id: range1969320
version: 1
direction: direction/forward
top level: BBa_K137059
start: 1279
end: 1319
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1969315
display id: annotation1969315
version: 1
top level: BBa_K137059
location: https://synbiohub.org/public/igem/BBa_K137059/annotation1969315/range1969315/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K137059/annotation1969315/range1969315/1
type: Range
persistent identity: range1969315
display id: range1969315
version: 1
direction: direction/reverse
top level: BBa_K137059
start: 445
end: 456
orientation: inline

BBa_K137008
type: SequenceAnnotation
persistent identity: annotation1969313
display id: annotation1969313
version: 1
top level: BBa_K137059
location: https://synbiohub.org/public/igem/BBa_K137059/annotation1969313/range1969313/1
component: BBa_K137008

https://synbiohub.org/public/igem/BBa_K137059/annotation1969313/range1969313/1
type: Range
persistent identity: range1969313
display id: range1969313
version: 1
direction: direction/reverse
top level: BBa_K137059
start: 402
end: 436
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation1969317
display id: annotation1969317
version: 1
top level: BBa_K137059
location: https://synbiohub.org/public/igem/BBa_K137059/annotation1969317/range1969317/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_K137059/annotation1969317/range1969317/1
type: Range
persistent identity: range1969317
display id: range1969317
version: 1
direction: direction/reverse
top level: BBa_K137059
start: 463
end: 1182
orientation: inline

BBa_K137048
type: SequenceAnnotation
persistent identity: annotation1969310
display id: annotation1969310
version: 1
top level: BBa_K137059
location: https://synbiohub.org/public/igem/BBa_K137059/annotation1969310/range1969310/1
component: BBa_K137048

https://synbiohub.org/public/igem/BBa_K137059/annotation1969310/range1969310/1
type: Range
persistent identity: range1969310
display id: range1969310
version: 1
direction: direction/reverse
top level: BBa_K137059
start: 44
end: 393
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1969318
display id: annotation1969318
version: 1
top level: BBa_K137059
location: https://synbiohub.org/public/igem/BBa_K137059/annotation1969318/range1969318/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K137059/annotation1969318/range1969318/1
type: Range
persistent identity: range1969318
display id: range1969318
version: 1
direction: direction/forward
top level: BBa_K137059
start: 1191
end: 1270
orientation: inline

BBa_K137010
type: SequenceAnnotation
persistent identity: annotation1969308
display id: annotation1969308
version: 1
top level: BBa_K137059
location: https://synbiohub.org/public/igem/BBa_K137059/annotation1969308/range1969308/1
component: BBa_K137010

https://synbiohub.org/public/igem/BBa_K137059/annotation1969308/range1969308/1
type: Range
persistent identity: range1969308
display id: range1969308
version: 1
direction: direction/reverse
top level: BBa_K137059
start: 1
end: 35
orientation: inline

BBa_K1632007
type: ComponentDefinition
persistent identity: BBa K1632007
display id: BBa_K1632007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: fim switch[default ON](wild-type)_rbs_gfp
creator: Riku Shinohara
created: 2015-08-29T11:00:00Z
modified: 2015-09-18T09:39:23Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2049_
m user id: 24760
owner id: 22687
owning group id: 9
status: status/Planning
bookmark: true
mutable description: a
mutable notes: a
mutable provenance: a
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_I13500
component: BBa_K1632004
sequence annotation: BBa_K1632004
sequence annotation: BBa_I13500
sequence: https://synbiohub.org/public/igem/BBa_K1632007_sequence/1

BBa_I13500
type: Component
persistent identity: component2448480
display id: component2448480
version: 1
top level: BBa_K1632007
access: public
definition: https://synbiohub.org/public/igem/BBa_I13500/1

BBa_K1632004
type: Component
persistent identity: component2448476
display id: component2448476
version: 1
top level: BBa_K1632007
access: public
definition: https://synbiohub.org/public/igem/BBa_K1632004/1

BBa_K1632004
type: SequenceAnnotation
persistent identity: annotation2448476
display id: annotation2448476
version: 1
top level: BBa_K1632007
location: https://synbiohub.org/public/igem/BBa_K1632007/annotation2448476/range2448476/1
component: BBa_K1632004

https://synbiohub.org/public/igem/BBa_K1632007/annotation2448476/range2448476/1
type: Range
persistent identity: range2448476
display id: range2448476
version: 1
direction: direction/reverse
top level: BBa_K1632007
start: 1
end: 382
orientation: inline

BBa_I13500
type: SequenceAnnotation
persistent identity: annotation2448480
display id: annotation2448480
version: 1
top level: BBa_K1632007
location: https://synbiohub.org/public/igem/BBa_K1632007/annotation2448480/range2448480/1
component: BBa_I13500

https://synbiohub.org/public/igem/BBa_K1632007/annotation2448480/range2448480/1
type: Range
persistent identity: range2448480
display id: range2448480
version: 1
direction: direction/reverse
top level: BBa_K1632007
start: 391
end: 1128
orientation: inline

BBa_K137057
type: ComponentDefinition
persistent identity: BBa K137057
display id: BBa_K137057
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: GFP fimE switch with 150 bp tetR promoter
creator: Allen Lin
created: 2008-07-30T11:00:00Z
modified: 2015-05-08T01:10:09Z
discontinued: false
dominant: false
experience: experience/Fails
group u list: _187_
m user id: 0
owner id: 3112
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: A promoter facing upstream is flanked by two FimE binding sites. GFP is downstream to this fimE switch. Active FimE will flip the promoter such that GFP is expresssed.
mutable notes: None.
mutable provenance: Previously constructed Biobrick parts.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_K137010
component: BBa_B0010
component: BBa_K137046
component: BBa_B0034
component: BBa_E0040
component: BBa_K137008
component: BBa_B0012
sequence annotation: BBa_E0040
sequence annotation: BBa_K137010
sequence annotation: BBa_K137046
sequence annotation: BBa_B0012
sequence annotation: BBa_B0010
sequence annotation: BBa_K137008
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K137057_sequence/1

BBa_K137010
type: Component
persistent identity: component1969272
display id: component1969272
version: 1
top level: BBa_K137057
access: public
definition: fimE IRL

BBa_B0010
type: Component
persistent identity: component1969282
display id: component1969282
version: 1
top level: BBa_K137057
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_K137046
type: Component
persistent identity: component1969274
display id: component1969274
version: 1
top level: BBa_K137057
access: public
definition: https://synbiohub.org/public/igem/BBa_K137046/1

BBa_B0034
type: Component
persistent identity: component1969279
display id: component1969279
version: 1
top level: BBa_K137057
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_E0040
type: Component
persistent identity: component1969281
display id: component1969281
version: 1
top level: BBa_K137057
access: public
definition: https://synbiohub.org/public/igem/BBa_E0040/1

BBa_K137008
type: Component
persistent identity: component1969277
display id: component1969277
version: 1
top level: BBa_K137057
access: public
definition: IRR

BBa_B0012
type: Component
persistent identity: component1969284
display id: component1969284
version: 1
top level: BBa_K137057
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation1969281
display id: annotation1969281
version: 1
top level: BBa_K137057
location: https://synbiohub.org/public/igem/BBa_K137057/annotation1969281/range1969281/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_K137057/annotation1969281/range1969281/1
type: Range
persistent identity: range1969281
display id: range1969281
version: 1
direction: direction/reverse
top level: BBa_K137057
start: 263
end: 982
orientation: inline

BBa_K137010
type: SequenceAnnotation
persistent identity: annotation1969272
display id: annotation1969272
version: 1
top level: BBa_K137057
location: https://synbiohub.org/public/igem/BBa_K137057/annotation1969272/range1969272/1
component: BBa_K137010

https://synbiohub.org/public/igem/BBa_K137057/annotation1969272/range1969272/1
type: Range
persistent identity: range1969272
display id: range1969272
version: 1
direction: direction/reverse
top level: BBa_K137057
start: 1
end: 35
orientation: inline

BBa_K137046
type: SequenceAnnotation
persistent identity: annotation1969274
display id: annotation1969274
version: 1
top level: BBa_K137057
location: https://synbiohub.org/public/igem/BBa_K137057/annotation1969274/range1969274/1
component: BBa_K137046

https://synbiohub.org/public/igem/BBa_K137057/annotation1969274/range1969274/1
type: Range
persistent identity: range1969274
display id: range1969274
version: 1
direction: direction/reverse
top level: BBa_K137057
start: 44
end: 193
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1969284
display id: annotation1969284
version: 1
top level: BBa_K137057
location: https://synbiohub.org/public/igem/BBa_K137057/annotation1969284/range1969284/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K137057/annotation1969284/range1969284/1
type: Range
persistent identity: range1969284
display id: range1969284
version: 1
direction: direction/forward
top level: BBa_K137057
start: 1079
end: 1119
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1969282
display id: annotation1969282
version: 1
top level: BBa_K137057
location: https://synbiohub.org/public/igem/BBa_K137057/annotation1969282/range1969282/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K137057/annotation1969282/range1969282/1
type: Range
persistent identity: range1969282
display id: range1969282
version: 1
direction: direction/forward
top level: BBa_K137057
start: 991
end: 1070
orientation: inline

BBa_K137008
type: SequenceAnnotation
persistent identity: annotation1969277
display id: annotation1969277
version: 1
top level: BBa_K137057
location: https://synbiohub.org/public/igem/BBa_K137057/annotation1969277/range1969277/1
component: BBa_K137008

https://synbiohub.org/public/igem/BBa_K137057/annotation1969277/range1969277/1
type: Range
persistent identity: range1969277
display id: range1969277
version: 1
direction: direction/reverse
top level: BBa_K137057
start: 202
end: 236
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1969279
display id: annotation1969279
version: 1
top level: BBa_K137057
location: https://synbiohub.org/public/igem/BBa_K137057/annotation1969279/range1969279/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K137057/annotation1969279/range1969279/1
type: Range
persistent identity: range1969279
display id: range1969279
version: 1
direction: direction/reverse
top level: BBa_K137057
start: 245
end: 256
orientation: inline

BBa_K1632000
type: ComponentDefinition
persistent identity: BBa K1632000
display id: BBa_K1632000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: fim switch[deault ON](Tokyo_Tech/J23119)
creator: Riku Shinohara
created: 2015-08-12T11:00:00Z
modified: 2015-09-15T12:53:44Z
discontinued: false
dominant: false
experience: experience/Issues
group u list: _2049_
m user id: 24760
owner id: 22687
owning group id: 9
status: status/Planning
bookmark: false
mutable description: a
mutable notes: a
mutable provenance: a
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: Sall
sequence annotation: J23119 promoter
sequence annotation: Ascl
sequence annotation: BamHl
sequence annotation: Bglll
sequence annotation: Mlul
sequence: https://synbiohub.org/public/igem/BBa_K1632000_sequence/1

Sall
type: SequenceAnnotation
persistent identity: annotation2435247
display id: annotation2435247
version: 1
top level: BBa_K1632000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1632000/annotation2435247/range2435247/1

https://synbiohub.org/public/igem/BBa_K1632000/annotation2435247/range2435247/1
type: Range
persistent identity: range2435247
display id: range2435247
version: 1
direction: direction/forward
top level: BBa_K1632000
start: 319
end: 324
orientation: inline

J23119 promoter
type: SequenceAnnotation
persistent identity: annotation2435250
display id: annotation2435250
version: 1
top level: BBa_K1632000
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1632000/annotation2435250/range2435250/1

https://synbiohub.org/public/igem/BBa_K1632000/annotation2435250/range2435250/1
type: Range
persistent identity: range2435250
display id: range2435250
version: 1
direction: direction/forward
top level: BBa_K1632000
start: 339
end: 373
orientation: inline

Ascl
type: SequenceAnnotation
persistent identity: annotation2435252
display id: annotation2435252
version: 1
top level: BBa_K1632000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1632000/annotation2435252/range2435252/1

https://synbiohub.org/public/igem/BBa_K1632000/annotation2435252/range2435252/1
type: Range
persistent identity: range2435252
display id: range2435252
version: 1
direction: direction/forward
top level: BBa_K1632000
start: 380
end: 387
orientation: inline

BamHl
type: SequenceAnnotation
persistent identity: annotation2435249
display id: annotation2435249
version: 1
top level: BBa_K1632000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1632000/annotation2435249/range2435249/1

https://synbiohub.org/public/igem/BBa_K1632000/annotation2435249/range2435249/1
type: Range
persistent identity: range2435249
display id: range2435249
version: 1
direction: direction/forward
top level: BBa_K1632000
start: 333
end: 338
orientation: inline

Ascl
type: SequenceAnnotation
persistent identity: annotation2435248
display id: annotation2435248
version: 1
top level: BBa_K1632000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1632000/annotation2435248/range2435248/1

https://synbiohub.org/public/igem/BBa_K1632000/annotation2435248/range2435248/1
type: Range
persistent identity: range2435248
display id: range2435248
version: 1
direction: direction/forward
top level: BBa_K1632000
start: 325
end: 332
orientation: inline

Bglll
type: SequenceAnnotation
persistent identity: annotation2435251
display id: annotation2435251
version: 1
top level: BBa_K1632000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1632000/annotation2435251/range2435251/1

https://synbiohub.org/public/igem/BBa_K1632000/annotation2435251/range2435251/1
type: Range
persistent identity: range2435251
display id: range2435251
version: 1
direction: direction/forward
top level: BBa_K1632000
start: 374
end: 379
orientation: inline

Mlul
type: SequenceAnnotation
persistent identity: annotation2435253
display id: annotation2435253
version: 1
top level: BBa_K1632000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1632000/annotation2435253/range2435253/1

https://synbiohub.org/public/igem/BBa_K1632000/annotation2435253/range2435253/1
type: Range
persistent identity: range2435253
display id: range2435253
version: 1
direction: direction/forward
top level: BBa_K1632000
start: 388
end: 392
orientation: inline

IRR
type: ComponentDefinition
persistent identity: BBa K137008
display id: BBa_K137008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: fimE IRR
creator: Allen Lin
created: 2008-06-19T11:00:00Z
modified: 2015-05-08T01:10:08Z
discontinued: false
dominant: false
experience: experience/Issues
group u list: _187_
m user id: 0
owner id: 3112
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: fimE inverted repeat right recombination site
mutable notes: none
mutable provenance: pFIP plasmid
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: IRR out
sequence annotation: IRR in
sequence: https://synbiohub.org/public/igem/BBa_K137008_sequence/1

IRR out
type: SequenceAnnotation
persistent identity: annotation1963841
display id: annotation1963841
version: 1
top level: IRR
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K137008/annotation1963841/range1963841/1

https://synbiohub.org/public/igem/BBa_K137008/annotation1963841/range1963841/1
type: Range
persistent identity: range1963841
display id: range1963841
version: 1
direction: direction/forward
top level: IRR
start: 1
end: 17
orientation: inline

IRR in
type: SequenceAnnotation
persistent identity: annotation1963842
display id: annotation1963842
version: 1
top level: IRR
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K137008/annotation1963842/range1963842/1

https://synbiohub.org/public/igem/BBa_K137008/annotation1963842/range1963842/1
type: Range
persistent identity: range1963842
display id: range1963842
version: 1
direction: direction/forward
top level: IRR
start: 19
end: 35
orientation: inline