//function/recombination/flp
type: Collection
persistent identity: function recombination flp
display id: function_recombination_flp
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
owned by: james
owned by: myers
member: BBa_J61027
member: BBa_J61024
member: BBa_K551000
member: FRT
member: BBa_J61025
member: BBa_J61026
member: BBa_K112138
member: BBa_K313002
member: pINDEL
member: BBa_K1015001

BBa_K551000
type: ComponentDefinition
persistent identity: BBa K551000
display id: BBa_K551000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: FRT-CM-FRT
creator: Laurence Van Melderen
created: 2011-09-18T11:00:00Z
modified: 2015-05-08T01:12:40Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _719_
m user id: 0
owner id: 4079
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This part is composed of two Flipase Recognition Targets (FRT) flanking the Chloramphenicol resistance gene.
mutable notes: The initial part wasn't working, so we remade it from the intermediate
mutable provenance: It comes from PCR of a registry part
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_J61020
component: BBa_P1004
sequence annotation: BBa_J61020
sequence annotation: BBa_P1004
sequence: https://synbiohub.org/public/igem/BBa_K551000_sequence/1

BBa_J61020
type: Component
persistent identity: component2136845
display id: component2136845
version: 1
top level: BBa_K551000
access: public
definition: FRT

BBa_J61020
type: Component
persistent identity: component2136842
display id: component2136842
version: 1
top level: BBa_K551000
access: public
definition: FRT

BBa_P1004
type: Component
persistent identity: component2136844
display id: component2136844
version: 1
top level: BBa_K551000
access: public
definition: https://synbiohub.org/public/igem/BBa_P1004/1

BBa_J61020
type: SequenceAnnotation
persistent identity: annotation2136842
display id: annotation2136842
version: 1
top level: BBa_K551000
location: https://synbiohub.org/public/igem/BBa_K551000/annotation2136842/range2136842/1
component: BBa_J61020

https://synbiohub.org/public/igem/BBa_K551000/annotation2136842/range2136842/1
type: Range
persistent identity: range2136842
display id: range2136842
version: 1
direction: direction/reverse
top level: BBa_K551000
start: 1
end: 34
orientation: inline

BBa_J61020
type: SequenceAnnotation
persistent identity: annotation2136845
display id: annotation2136845
version: 1
top level: BBa_K551000
location: https://synbiohub.org/public/igem/BBa_K551000/annotation2136845/range2136845/1
component: BBa_J61020

https://synbiohub.org/public/igem/BBa_K551000/annotation2136845/range2136845/1
type: Range
persistent identity: range2136845
display id: range2136845
version: 1
direction: direction/reverse
top level: BBa_K551000
start: 804
end: 837
orientation: inline

BBa_P1004
type: SequenceAnnotation
persistent identity: annotation2136844
display id: annotation2136844
version: 1
top level: BBa_K551000
location: https://synbiohub.org/public/igem/BBa_K551000/annotation2136844/range2136844/1
component: BBa_P1004

https://synbiohub.org/public/igem/BBa_K551000/annotation2136844/range2136844/1
type: Range
persistent identity: range2136844
display id: range2136844
version: 1
direction: direction/reverse
top level: BBa_K551000
start: 35
end: 803
orientation: inline

BBa_J61024
type: ComponentDefinition
persistent identity: BBa J61024
display id: BBa_J61024
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: [CmR][FRT]
creator: John Anderson
created: 2006-09-20T11:00:00Z
modified: 2015-08-31T02:02:59Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Intermediate for knockin or knockout cassettes with chloramphenicol selection. The cassettes can be removed by introduction of flp recombinase expressing helper plasmid pCP20 leaving behind a single FRT site scar.
mutable notes: N/A
mutable provenance: Standard biobrick assembly
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_J61020
component: BBa_J61000
sequence annotation: BBa_J61000
sequence annotation: BBa_J61020
sequence: https://synbiohub.org/public/igem/BBa_J61024_sequence/1

BBa_J61020
type: Component
persistent identity: component1902241
display id: component1902241
version: 1
top level: BBa_J61024
access: public
definition: FRT

BBa_J61000
type: Component
persistent identity: component1902240
display id: component1902240
version: 1
top level: BBa_J61024
access: public
definition: https://synbiohub.org/public/igem/BBa_J61000/1

BBa_J61000
type: SequenceAnnotation
persistent identity: annotation1902240
display id: annotation1902240
version: 1
top level: BBa_J61024
location: https://synbiohub.org/public/igem/BBa_J61024/annotation1902240/range1902240/1
component: BBa_J61000

https://synbiohub.org/public/igem/BBa_J61024/annotation1902240/range1902240/1
type: Range
persistent identity: range1902240
display id: range1902240
version: 1
direction: direction/reverse
top level: BBa_J61024
start: 1
end: 894
orientation: inline

BBa_J61020
type: SequenceAnnotation
persistent identity: annotation1902241
display id: annotation1902241
version: 1
top level: BBa_J61024
location: https://synbiohub.org/public/igem/BBa_J61024/annotation1902241/range1902241/1
component: BBa_J61020

https://synbiohub.org/public/igem/BBa_J61024/annotation1902241/range1902241/1
type: Range
persistent identity: range1902241
display id: range1902241
version: 1
direction: direction/reverse
top level: BBa_J61024
start: 903
end: 936
orientation: inline

pINDEL
type: ComponentDefinition
persistent identity: BBa K551001
display id: BBa_K551001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: plasmid of insertion and deletion
creator: ULB-Brussels team
created: 2011-09-18T11:00:00Z
modified: 2015-05-08T01:12:40Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _719_
m user id: 0
owner id: 4079
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: Plasmid of insertion and deletion of genes
mutable notes: The thermosensitive origin of replication made it slow to grow
mutable provenance: It comes from pKD46 which was modified by adding the flipase function to it
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000155
role: partType/Plasmid
sequence annotation: OriR101
sequence annotation: RepA101ts
sequence annotation: gam
sequence annotation: CI857ts
sequence annotation: pARAb
sequence annotation: AraC
sequence annotation: Flipase
sequence annotation: URA3
sequence annotation: AmpR
sequence annotation: bet
sequence annotation: exo
sequence: https://synbiohub.org/public/igem/BBa_K551001_sequence/1

OriR101
type: SequenceAnnotation
persistent identity: annotation2136556
display id: annotation2136556
version: 1
top level: pINDEL
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K551001/annotation2136556/range2136556/1

https://synbiohub.org/public/igem/BBa_K551001/annotation2136556/range2136556/1
type: Range
persistent identity: range2136556
display id: range2136556
version: 1
direction: direction/forward
top level: pINDEL
start: 8894
end: 9634
orientation: inline

RepA101ts
type: SequenceAnnotation
persistent identity: annotation2136517
display id: annotation2136517
version: 1
top level: pINDEL
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K551001/annotation2136517/range2136517/1

https://synbiohub.org/public/igem/BBa_K551001/annotation2136517/range2136517/1
type: Range
persistent identity: range2136517
display id: range2136517
version: 1
direction: direction/reverse
top level: pINDEL
start: 7936
end: 8889
orientation: inline

gam
type: SequenceAnnotation
persistent identity: annotation2136512
display id: annotation2136512
version: 1
top level: pINDEL
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K551001/annotation2136512/range2136512/1

https://synbiohub.org/public/igem/BBa_K551001/annotation2136512/range2136512/1
type: Range
persistent identity: range2136512
display id: range2136512
version: 1
direction: direction/forward
top level: pINDEL
start: 3737
end: 4152
orientation: inline

CI857ts
type: SequenceAnnotation
persistent identity: annotation2136516
display id: annotation2136516
version: 1
top level: pINDEL
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K551001/annotation2136516/range2136516/1

https://synbiohub.org/public/igem/BBa_K551001/annotation2136516/range2136516/1
type: Range
persistent identity: range2136516
display id: range2136516
version: 1
direction: direction/forward
top level: pINDEL
start: 7076
end: 7890
orientation: inline

pARAb
type: SequenceAnnotation
persistent identity: annotation2136557
display id: annotation2136557
version: 1
top level: pINDEL
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K551001/annotation2136557/range2136557/1

https://synbiohub.org/public/igem/BBa_K551001/annotation2136557/range2136557/1
type: Range
persistent identity: range2136557
display id: range2136557
version: 1
direction: direction/forward
top level: pINDEL
start: 3658
end: 3685
orientation: inline

AraC
type: SequenceAnnotation
persistent identity: annotation2136511
display id: annotation2136511
version: 1
top level: pINDEL
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K551001/annotation2136511/range2136511/1

https://synbiohub.org/public/igem/BBa_K551001/annotation2136511/range2136511/1
type: Range
persistent identity: range2136511
display id: range2136511
version: 1
direction: direction/reverse
top level: pINDEL
start: 2504
end: 3392
orientation: inline

Flipase
type: SequenceAnnotation
persistent identity: annotation2136515
display id: annotation2136515
version: 1
top level: pINDEL
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K551001/annotation2136515/range2136515/1

https://synbiohub.org/public/igem/BBa_K551001/annotation2136515/range2136515/1
type: Range
persistent identity: range2136515
display id: range2136515
version: 1
direction: direction/reverse
top level: pINDEL
start: 5805
end: 7076
orientation: inline

URA3
type: SequenceAnnotation
persistent identity: annotation2136510
display id: annotation2136510
version: 1
top level: pINDEL
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K551001/annotation2136510/range2136510/1

https://synbiohub.org/public/igem/BBa_K551001/annotation2136510/range2136510/1
type: Range
persistent identity: range2136510
display id: range2136510
version: 1
direction: direction/reverse
top level: pINDEL
start: 707
end: 1510
orientation: inline

AmpR
type: SequenceAnnotation
persistent identity: annotation2136509
display id: annotation2136509
version: 1
top level: pINDEL
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K551001/annotation2136509/range2136509/1

https://synbiohub.org/public/igem/BBa_K551001/annotation2136509/range2136509/1
type: Range
persistent identity: range2136509
display id: range2136509
version: 1
direction: direction/reverse
top level: pINDEL
start: 9818
end: 10678
orientation: inline

bet
type: SequenceAnnotation
persistent identity: annotation2136513
display id: annotation2136513
version: 1
top level: pINDEL
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K551001/annotation2136513/range2136513/1

https://synbiohub.org/public/igem/BBa_K551001/annotation2136513/range2136513/1
type: Range
persistent identity: range2136513
display id: range2136513
version: 1
direction: direction/forward
top level: pINDEL
start: 4159
end: 4944
orientation: inline

exo
type: SequenceAnnotation
persistent identity: annotation2136514
display id: annotation2136514
version: 1
top level: pINDEL
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K551001/annotation2136514/range2136514/1

https://synbiohub.org/public/igem/BBa_K551001/annotation2136514/range2136514/1
type: Range
persistent identity: range2136514
display id: range2136514
version: 1
direction: direction/forward
top level: pINDEL
start: 4941
end: 5621
orientation: inline

FRT
type: ComponentDefinition
persistent identity: BBa J72001
display id: BBa_J72001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: {FRT} recombination site for flp recombinase in BBb
creator: John Anderson
created: 2008-07-14T11:00:00Z
modified: 2015-05-08T01:08:26Z
discontinued: false
dominant: false
experience: experience/None
group u list: _171_
m user id: 0
owner id: 483
owning group id: 171
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Later
mutable notes: N/A
mutable provenance: Later
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_J72001_sequence/1

FRT
type: ComponentDefinition
persistent identity: BBa J61020
display id: BBa_J61020
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: [FRT]
creator: John Anderson
created: 2006-09-20T11:00:00Z
modified: 2015-08-31T02:02:59Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Site for recombination by flp recombinase. Genes flanked by FRT sites (oriented in the same direction) in the genome can be excised with the introduction of flp recombinase-expressing helper plasmid pCP20. Note that the original FRT sequence contained an XbaI site that has been removed with a point mutation for compatibility with standard assembly. See Datsenko and Wanner for details of its use in markerless knockouts and knockins.
mutable notes: N/A
mutable provenance: Extend ca1010F/R (73 bp, EcoRI/SpeI) Sub into pSB1A2-I13522 (EcoRI/SpeI) Product is pSB1A2-Bca1010 ---- ca1010F Forward (universal biobrick) EcoRI for FRT GGACTgaattcgcggccgcttctagag ca1010R Reverse SpeI oligo for FRT cctatactagtagaagttcctattctctaAaaagtataggaacttcctctagaagcggccgcg
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_J61020_sequence/1

BBa_K313002
type: ComponentDefinition
persistent identity: BBa K313002
display id: BBa_K313002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: flpe DNA recombinase
creator: Kohaku So, Takashi Hiroi, Megumi Iseki, Mami Nakatake, Ryosuke Kamei, Ryo Kariyazono
created: 2010-10-13T11:00:00Z
modified: 2015-05-08T01:11:54Z
discontinued: false
dominant: false
experience: experience/None
group u list: _433_
m user id: 0
owner id: 5929
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Flpe is a site-specific DNA recombinase that that recognizes FRT sequence. Flpe was derived from yeaset site-specific DNA recombinase Flp. (Buchholz, et al.,Improved properties of FLP recombinase evolved by cycling mutagenesis. Nature. Biotechnology, 16:657-662 ( 1998 )) It's internal EcoRI and SpeI site has been removed using site-directed mutagenesis.
mutable notes: It's internal EcoRI and SpeI site has been removed using site-directed mutagenesis.
mutable provenance: E.coli EL250 strain
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K313002_sequence/1

BBa_J61025
type: ComponentDefinition
persistent identity: BBa J61025
display id: BBa_J61025
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: [FRT][CmR][FRT]
creator: John Anderson
created: 2006-09-20T11:00:00Z
modified: 2015-08-31T02:02:59Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Cassette for knockins or knockouts with chloramphenicol selection. The cassette can be removed by introduction of flp recombinase expressing helper plasmid pCP20 leaving behind a single FRT site scar.
mutable notes: N/A
mutable provenance: Standard biobrick assembly
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_J61020
component: BBa_J61000
sequence annotation: BBa_J61020
sequence annotation: BBa_J61000
sequence: https://synbiohub.org/public/igem/BBa_J61025_sequence/1

BBa_J61020
type: Component
persistent identity: component1902232
display id: component1902232
version: 1
top level: BBa_J61025
access: public
definition: FRT

BBa_J61000
type: Component
persistent identity: component1902234
display id: component1902234
version: 1
top level: BBa_J61025
access: public
definition: https://synbiohub.org/public/igem/BBa_J61000/1

BBa_J61020
type: Component
persistent identity: component1902235
display id: component1902235
version: 1
top level: BBa_J61025
access: public
definition: FRT

BBa_J61020
type: SequenceAnnotation
persistent identity: annotation1902235
display id: annotation1902235
version: 1
top level: BBa_J61025
location: https://synbiohub.org/public/igem/BBa_J61025/annotation1902235/range1902235/1
component: BBa_J61020

https://synbiohub.org/public/igem/BBa_J61025/annotation1902235/range1902235/1
type: Range
persistent identity: range1902235
display id: range1902235
version: 1
direction: direction/reverse
top level: BBa_J61025
start: 945
end: 978
orientation: inline

BBa_J61020
type: SequenceAnnotation
persistent identity: annotation1902232
display id: annotation1902232
version: 1
top level: BBa_J61025
location: https://synbiohub.org/public/igem/BBa_J61025/annotation1902232/range1902232/1
component: BBa_J61020

https://synbiohub.org/public/igem/BBa_J61025/annotation1902232/range1902232/1
type: Range
persistent identity: range1902232
display id: range1902232
version: 1
direction: direction/reverse
top level: BBa_J61025
start: 1
end: 34
orientation: inline

BBa_J61000
type: SequenceAnnotation
persistent identity: annotation1902234
display id: annotation1902234
version: 1
top level: BBa_J61025
location: https://synbiohub.org/public/igem/BBa_J61025/annotation1902234/range1902234/1
component: BBa_J61000

https://synbiohub.org/public/igem/BBa_J61025/annotation1902234/range1902234/1
type: Range
persistent identity: range1902234
display id: range1902234
version: 1
direction: direction/reverse
top level: BBa_J61025
start: 43
end: 936
orientation: inline

BBa_J61027
type: ComponentDefinition
persistent identity: BBa J61027
display id: BBa_J61027
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: [FRT][R6K][CmR][FRT]
creator: John Anderson
created: 2006-09-20T11:00:00Z
modified: 2015-08-31T02:02:59Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Cassette for knockins or knockouts with chloramphenicol selection and a silent origin of replication. The cassettes can be removed by introduction of flp recombinase expressing helper plasmid pCP20 leaving behind a single FRT site scar. Digestion of the genomic DNA containing these cassettes followed by ligation and transformation into a pir116 or pir+ strain allows capture of the cassette as a high or medium-copy plasmid. pSB1A2-Bca9008
mutable notes: N/A
mutable provenance: standard biobrick assembly
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_J61020
component: BBa_J61000
component: BBa_J61001
sequence annotation: BBa_J61020
sequence annotation: BBa_J61001
sequence annotation: BBa_J61000
sequence: https://synbiohub.org/public/igem/BBa_J61027_sequence/1

BBa_J61020
type: Component
persistent identity: component1902242
display id: component1902242
version: 1
top level: BBa_J61027
access: public
definition: FRT

BBa_J61020
type: Component
persistent identity: component1902247
display id: component1902247
version: 1
top level: BBa_J61027
access: public
definition: FRT

BBa_J61000
type: Component
persistent identity: component1902246
display id: component1902246
version: 1
top level: BBa_J61027
access: public
definition: https://synbiohub.org/public/igem/BBa_J61000/1

BBa_J61001
type: Component
persistent identity: component1902244
display id: component1902244
version: 1
top level: BBa_J61027
access: public
definition: https://synbiohub.org/public/igem/BBa_J61001/1

BBa_J61020
type: SequenceAnnotation
persistent identity: annotation1902247
display id: annotation1902247
version: 1
top level: BBa_J61027
location: https://synbiohub.org/public/igem/BBa_J61027/annotation1902247/range1902247/1
component: BBa_J61020

https://synbiohub.org/public/igem/BBa_J61027/annotation1902247/range1902247/1
type: Range
persistent identity: range1902247
display id: range1902247
version: 1
direction: direction/reverse
top level: BBa_J61027
start: 1359
end: 1392
orientation: inline

BBa_J61001
type: SequenceAnnotation
persistent identity: annotation1902244
display id: annotation1902244
version: 1
top level: BBa_J61027
location: https://synbiohub.org/public/igem/BBa_J61027/annotation1902244/range1902244/1
component: BBa_J61001

https://synbiohub.org/public/igem/BBa_J61027/annotation1902244/range1902244/1
type: Range
persistent identity: range1902244
display id: range1902244
version: 1
direction: direction/reverse
top level: BBa_J61027
start: 43
end: 448
orientation: inline

BBa_J61020
type: SequenceAnnotation
persistent identity: annotation1902242
display id: annotation1902242
version: 1
top level: BBa_J61027
location: https://synbiohub.org/public/igem/BBa_J61027/annotation1902242/range1902242/1
component: BBa_J61020

https://synbiohub.org/public/igem/BBa_J61027/annotation1902242/range1902242/1
type: Range
persistent identity: range1902242
display id: range1902242
version: 1
direction: direction/reverse
top level: BBa_J61027
start: 1
end: 34
orientation: inline

BBa_J61000
type: SequenceAnnotation
persistent identity: annotation1902246
display id: annotation1902246
version: 1
top level: BBa_J61027
location: https://synbiohub.org/public/igem/BBa_J61027/annotation1902246/range1902246/1
component: BBa_J61000

https://synbiohub.org/public/igem/BBa_J61027/annotation1902246/range1902246/1
type: Range
persistent identity: range1902246
display id: range1902246
version: 1
direction: direction/reverse
top level: BBa_J61027
start: 457
end: 1350
orientation: inline

BBa_K1015001
type: ComponentDefinition
persistent identity: BBa K1015001
display id: BBa_K1015001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Flippase(FLP)
creator: Kento Tominaga
created: 2013-09-14T11:00:00Z
modified: 2015-05-08T01:08:42Z
discontinued: false
dominant: false
group u list: _1321_
m user id: 0
owner id: 15618
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: site specific recombinase that recognize FRT site. FLP catalyze site specific recombination between two FRT site.
mutable notes: site specific recombination between two FRT site.
mutable provenance: 2??m plasmid of Saccharomyces cerevisiae.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: FLP
sequence: https://synbiohub.org/public/igem/BBa_K1015001_sequence/1

FLP
type: SequenceAnnotation
persistent identity: annotation2342868
display id: annotation2342868
version: 1
top level: BBa_K1015001
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1015001/annotation2342868/range2342868/1

https://synbiohub.org/public/igem/BBa_K1015001/annotation2342868/range2342868/1
type: Range
persistent identity: range2342868
display id: range2342868
version: 1
direction: direction/forward
top level: BBa_K1015001
start: 1
end: 1272
orientation: inline

BBa_K112138
type: ComponentDefinition
persistent identity: BBa K112138
display id: BBa_K112138
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: FRT-KanR-R6K-FRT-attP21
creator: Madhvi Venkatesh
created: 2008-10-31T12:00:00Z
modified: 2015-05-08T01:09:14Z
discontinued: false
dominant: false
group u list: _224_
m user id: 0
owner id: 3023
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: FRT-KanR-R6K-FRT-attP21
mutable notes: This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at: BBb Standard Description Page
mutable provenance: Anderson lab vectors
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Other
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_K112138_sequence/1

BBa_J61026
type: ComponentDefinition
persistent identity: BBa J61026
display id: BBa_J61026
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: [FRT][R6K]
creator: John Anderson
created: 2006-09-20T11:00:00Z
modified: 2015-08-31T02:02:59Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Intermediate for knockin or knockout cassettes with a silent origin of replication. The cassettes can be removed by introduction of flp recombinase expressing helper plasmid pCP20 leaving behind a single FRT site scar. Digestion of the genomic DNA containing these cassettes followed by ligation and transformation into a pir116 or pir+ strain allows capture of the cassette as a high or medium-copy plasmid. pSB1A2-Bca9004
mutable notes: N/A
mutable provenance: standard biobrick assembly
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_J61001
component: BBa_J61020
sequence annotation: BBa_J61001
sequence annotation: BBa_J61020
sequence: https://synbiohub.org/public/igem/BBa_J61026_sequence/1

BBa_J61001
type: Component
persistent identity: component1902238
display id: component1902238
version: 1
top level: BBa_J61026
access: public
definition: https://synbiohub.org/public/igem/BBa_J61001/1

BBa_J61020
type: Component
persistent identity: component1902236
display id: component1902236
version: 1
top level: BBa_J61026
access: public
definition: FRT

BBa_J61001
type: SequenceAnnotation
persistent identity: annotation1902238
display id: annotation1902238
version: 1
top level: BBa_J61026
location: https://synbiohub.org/public/igem/BBa_J61026/annotation1902238/range1902238/1
component: BBa_J61001

https://synbiohub.org/public/igem/BBa_J61026/annotation1902238/range1902238/1
type: Range
persistent identity: range1902238
display id: range1902238
version: 1
direction: direction/reverse
top level: BBa_J61026
start: 43
end: 448
orientation: inline

BBa_J61020
type: SequenceAnnotation
persistent identity: annotation1902236
display id: annotation1902236
version: 1
top level: BBa_J61026
location: https://synbiohub.org/public/igem/BBa_J61026/annotation1902236/range1902236/1
component: BBa_J61020

https://synbiohub.org/public/igem/BBa_J61026/annotation1902236/range1902236/1
type: Range
persistent identity: range1902236
display id: range1902236
version: 1
direction: direction/reverse
top level: BBa_J61026
start: 1
end: 34
orientation: inline