//function/recombination/hin
type: Collection
persistent identity: function recombination hin
display id: function_recombination_hin
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
owned by: james
owned by: myers
member: BBa_J44013
member: BBa_I715042
member: BBa_I715049
member: Hin
member: BBa_J3104
member: BBa_I715047
member: BBa_J3106
member: BBa_I715044
member: BBa_J44009
member: BBa_J44003
member: BBa_J44010
member: BBa_I715051
member: BBa_J44012
member: BBa_I715043
member: Hin LVA
member: BBa_J3105
member: BBa_S03639
member: BBa_J3107
member: BBa_J3108
member: BBa_J3103
member: BBa_J44011
member: BBa_I715037
member: RE
member: hixC

BBa_I715049
type: ComponentDefinition
persistent identity: BBa I715049
display id: BBa_I715049
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: HPP-A1 + Hin
creator: Amanda Valencia
created: 2007-10-23T11:00:00Z
modified: 2015-08-31T04:07:50Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _120_
m user id: 0
owner id: 1858
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: Part is composed of a Hin recombinase expression casette driven by pLac and series of flippable units that include halves of GFP and RFP: T7prom-RBS-RFP1-hixC-RFP2-RBS-GFP1-hixC-RFP2-TT-hixC-GFP2-TT-hixC . The corresponding part without Hin glows redas a result of the expression of the hix-interrupted RFP. With Hin, most colonies are red, but green colonies appear. Evidence suggests that this is a result of Hin-mediated recombination.
mutable notes: None
mutable provenance: Ligation of existing part
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_J44000
component: BBa_I715020
component: BBa_B0034
component: BBa_I715022
component: BBa_I715023
component: BBa_B0030
component: BBa_B0012
component: BBa_B0010
component: BBa_R0010
component: BBa_I715019
component: BBa_J31001
component: BBa_R0085
sequence annotation: BBa_B0012
sequence annotation: BBa_B0034
sequence annotation: BBa_J44000
sequence annotation: BBa_J31001
sequence annotation: BBa_R0085
sequence annotation: BBa_B0010
sequence annotation: BBa_I715019
sequence annotation: BBa_I715022
sequence annotation: BBa_I715020
sequence annotation: BBa_B0030
sequence annotation: BBa_I715023
sequence annotation: BBa_R0010
sequence: https://synbiohub.org/public/igem/BBa_I715049_sequence/1

BBa_J44000
type: Component
persistent identity: component1954191
display id: component1954191
version: 1
top level: BBa_I715049
access: public
definition: hixC

BBa_I715020
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display id: component1954213
version: 1
top level: BBa_I715049
access: public
definition: https://synbiohub.org/public/igem/BBa_I715020/1

BBa_B0034
type: Component
persistent identity: component1954196
display id: component1954196
version: 1
top level: BBa_I715049
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_I715022
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persistent identity: component1954190
display id: component1954190
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access: public
definition: https://synbiohub.org/public/igem/BBa_I715022/1

BBa_I715023
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persistent identity: component1954203
display id: component1954203
version: 1
top level: BBa_I715049
access: public
definition: https://synbiohub.org/public/igem/BBa_I715023/1

BBa_B0030
type: Component
persistent identity: component1954168
display id: component1954168
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access: public
definition: https://synbiohub.org/public/igem/BBa_B0030/1

BBa_B0012
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persistent identity: component1954216
display id: component1954216
version: 1
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access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0012
type: Component
persistent identity: component1954178
display id: component1954178
version: 1
top level: BBa_I715049
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_J44000
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display id: component1954210
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definition: hixC

BBa_B0010
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display id: component1954204
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definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0012
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persistent identity: component1954206
display id: component1954206
version: 1
top level: BBa_I715049
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_R0010
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display id: component1954160
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access: public
definition: https://synbiohub.org/public/igem/BBa_R0010/1

BBa_J44000
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display id: component1954200
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top level: BBa_I715049
access: public
definition: hixC

BBa_I715019
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display id: component1954199
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access: public
definition: https://synbiohub.org/public/igem/BBa_I715019/1

BBa_I715023
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display id: component1954194
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top level: BBa_I715049
access: public
definition: https://synbiohub.org/public/igem/BBa_I715023/1

BBa_J44000
type: Component
persistent identity: component1954220
display id: component1954220
version: 1
top level: BBa_I715049
access: public
definition: hixC

BBa_J31001
type: Component
persistent identity: component1954175
display id: component1954175
version: 1
top level: BBa_I715049
access: public
definition: Hin LVA

BBa_B0010
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persistent identity: component1954176
display id: component1954176
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top level: BBa_I715049
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_R0085
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persistent identity: component1954185
display id: component1954185
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access: public
definition: https://synbiohub.org/public/igem/BBa_R0085/1

BBa_B0010
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display id: component1954214
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access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0034
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display id: component1954187
version: 1
top level: BBa_I715049
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0012
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component: BBa_B0012

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BBa_B0034
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BBa_J31001
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BBa_R0085
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BBa_B0010
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BBa_I715019
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display id: annotation1954199
version: 1
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BBa_B0034
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BBa_I715022
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BBa_I715020
type: SequenceAnnotation
persistent identity: annotation1954213
display id: annotation1954213
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top level: BBa_I715049
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persistent identity: range1954213
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BBa_B0012
type: SequenceAnnotation
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display id: annotation1954178
version: 1
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component: BBa_B0012

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BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1954204
display id: annotation1954204
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component: BBa_B0010

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persistent identity: range1954204
display id: range1954204
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direction: direction/forward
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BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1954214
display id: annotation1954214
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component: BBa_B0010

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persistent identity: range1954214
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BBa_J44000
type: SequenceAnnotation
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persistent identity: range1954191
display id: range1954191
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BBa_B0012
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display id: annotation1954206
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persistent identity: range1954206
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BBa_B0030
type: SequenceAnnotation
persistent identity: annotation1954168
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BBa_I715023
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BBa_I715023
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BBa_R0010
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BBa_J44000
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BBa_J44000
type: SequenceAnnotation
persistent identity: annotation1954220
display id: annotation1954220
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BBa_J3107
type: ComponentDefinition
persistent identity: BBa J3107
display id: BBa_J3107
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: AraC-PC, pLac-Hin
creator: Karmella Haynes
created: 2006-10-11T11:00:00Z
modified: 2015-08-31T04:08:45Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _61_
m user id: 0
owner id: 1144
owning group id: 61
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: AraC-PC (part BBa_I13458) should constitutively express the AraC protein (inhibitor of the pBad promoter). Expression of Hin invertase is under control of the pLac promoter. Therefore, Hin should be expressed in the presence of IPTG. This part was designed for two reasons: 1. To regulate DNA inversion (via controlled Hin invertase expression) on a second plasmid 2. To regulate the transcription of a pBad driven reporter (via the addition of arabinose) on the second plasmid
mutable notes: A double forward terminator is placed upstream of "pLac-Hin" to block forward read-through transcription from the pSB1AK3 vector. This should allow tighter control over Hin expression.
mutable provenance: AraC-PC-TT - part BBa_S03591, a composite of BBa_I13458 and part BBa_B0015 pLac-RBS-Hin-TT - part BBa_S03537
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0012
component: BBa_B0030
component: BBa_I13458
component: BBa_B0010
component: BBa_R0010
component: BBa_J31000
sequence annotation: BBa_J31000
sequence annotation: BBa_B0030
sequence annotation: BBa_B0012
sequence annotation: BBa_R0010
sequence annotation: BBa_I13458
sequence annotation: BBa_B0010
sequence: https://synbiohub.org/public/igem/BBa_J3107_sequence/1

BBa_B0012
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display id: component1902760
version: 1
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access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0030
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access: public
definition: https://synbiohub.org/public/igem/BBa_B0030/1

BBa_I13458
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display id: component1902757
version: 1
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access: public
definition: https://synbiohub.org/public/igem/BBa_I13458/1

BBa_B0010
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definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0010
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access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_R0010
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persistent identity: component1902764
display id: component1902764
version: 1
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access: public
definition: https://synbiohub.org/public/igem/BBa_R0010/1

BBa_B0012
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access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_J31000
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persistent identity: component1902777
display id: component1902777
version: 1
top level: BBa_J3107
access: public
definition: Hin

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BBa_B0012
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version: 1
top level: BBa_J3107
location: https://synbiohub.org/public/igem/BBa_J3107/annotation1902764/range1902764/1
component: BBa_R0010

https://synbiohub.org/public/igem/BBa_J3107/annotation1902764/range1902764/1
type: Range
persistent identity: range1902764
display id: range1902764
version: 1
direction: direction/forward
top level: BBa_J3107
start: 1260
end: 1459
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1902780
display id: annotation1902780
version: 1
top level: BBa_J3107
location: https://synbiohub.org/public/igem/BBa_J3107/annotation1902780/range1902780/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_J3107/annotation1902780/range1902780/1
type: Range
persistent identity: range1902780
display id: range1902780
version: 1
direction: direction/forward
top level: BBa_J3107
start: 2158
end: 2198
orientation: inline

BBa_I13458
type: SequenceAnnotation
persistent identity: annotation1902757
display id: annotation1902757
version: 1
top level: BBa_J3107
location: https://synbiohub.org/public/igem/BBa_J3107/annotation1902757/range1902757/1
component: BBa_I13458

https://synbiohub.org/public/igem/BBa_J3107/annotation1902757/range1902757/1
type: Range
persistent identity: range1902757
display id: range1902757
version: 1
direction: direction/reverse
top level: BBa_J3107
start: 1
end: 1114
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1902778
display id: annotation1902778
version: 1
top level: BBa_J3107
location: https://synbiohub.org/public/igem/BBa_J3107/annotation1902778/range1902778/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_J3107/annotation1902778/range1902778/1
type: Range
persistent identity: range1902778
display id: range1902778
version: 1
direction: direction/forward
top level: BBa_J3107
start: 2070
end: 2149
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1902758
display id: annotation1902758
version: 1
top level: BBa_J3107
location: https://synbiohub.org/public/igem/BBa_J3107/annotation1902758/range1902758/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_J3107/annotation1902758/range1902758/1
type: Range
persistent identity: range1902758
display id: range1902758
version: 1
direction: direction/forward
top level: BBa_J3107
start: 1123
end: 1202
orientation: inline

RE
type: ComponentDefinition
persistent identity: BBa J3101
display id: BBa_J3101
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Recombinational Enhancer (RE) for Hin/Hix inverting
creator: Erin Zwack, Sabriya Rosemond
created: 2006-06-01T11:00:00Z
modified: 2015-08-31T04:08:45Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _61_
m user id: 0
owner id: 918
owning group id: 61
sample status: In stock
status: status/Available
bookmark: true
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: Originally a C
sequence annotation: Mutation of SpeI site
sequence annotation: Insertion right before biobrick ends
sequence annotation: Proximal Fis Binding Site
sequence annotation: Distal Fis Binding Site
sequence annotation: RE Sequence
sequence annotation: Former SpeI site
sequence: https://synbiohub.org/public/igem/BBa_J3101_sequence/1

Originally a C
type: SequenceAnnotation
persistent identity: annotation1884989
display id: annotation1884989
version: 1
top level: RE
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J3101/annotation1884989/range1884989/1

https://synbiohub.org/public/igem/BBa_J3101/annotation1884989/range1884989/1
type: Range
persistent identity: range1884989
display id: range1884989
version: 1
direction: direction/forward
top level: RE
start: 29
end: 29
orientation: inline

Mutation of SpeI site
type: SequenceAnnotation
persistent identity: annotation1880472
display id: annotation1880472
version: 1
top level: RE
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J3101/annotation1880472/range1880472/1

https://synbiohub.org/public/igem/BBa_J3101/annotation1880472/range1880472/1
type: Range
persistent identity: range1880472
display id: range1880472
version: 1
direction: direction/forward
top level: RE
start: 51
end: 51
orientation: inline

Insertion right before biobrick ends
type: SequenceAnnotation
persistent identity: annotation1884990
display id: annotation1884990
version: 1
top level: RE
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J3101/annotation1884990/range1884990/1

https://synbiohub.org/public/igem/BBa_J3101/annotation1884990/range1884990/1
type: Range
persistent identity: range1884990
display id: range1884990
version: 1
direction: direction/forward
top level: RE
start: 77
end: 77
orientation: inline

Proximal Fis Binding Site
type: SequenceAnnotation
persistent identity: annotation1884991
display id: annotation1884991
version: 1
top level: RE
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_J3101/annotation1884991/range1884991/1

https://synbiohub.org/public/igem/BBa_J3101/annotation1884991/range1884991/1
type: Range
persistent identity: range1884991
display id: range1884991
version: 1
direction: direction/forward
top level: RE
start: 6
end: 21
orientation: inline

Distal Fis Binding Site
type: SequenceAnnotation
persistent identity: annotation1884992
display id: annotation1884992
version: 1
top level: RE
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_J3101/annotation1884992/range1884992/1

https://synbiohub.org/public/igem/BBa_J3101/annotation1884992/range1884992/1
type: Range
persistent identity: range1884992
display id: range1884992
version: 1
direction: direction/forward
top level: RE
start: 55
end: 69
orientation: inline

RE Sequence
type: SequenceAnnotation
persistent identity: annotation1884988
display id: annotation1884988
version: 1
top level: RE
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J3101/annotation1884988/range1884988/1

https://synbiohub.org/public/igem/BBa_J3101/annotation1884988/range1884988/1
type: Range
persistent identity: range1884988
display id: range1884988
version: 1
direction: direction/forward
top level: RE
start: 1
end: 77
orientation: inline

Former SpeI site
type: SequenceAnnotation
persistent identity: annotation1880471
display id: annotation1880471
version: 1
top level: RE
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J3101/annotation1880471/range1880471/1

https://synbiohub.org/public/igem/BBa_J3101/annotation1880471/range1880471/1
type: Range
persistent identity: range1880471
display id: range1880471
version: 1
direction: direction/forward
top level: RE
start: 51
end: 56
orientation: inline

BBa_J3103
type: ComponentDefinition
persistent identity: BBa J3103
display id: BBa_J3103
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pBad-HixC-RBS-TetF-HixC-TT-RE
creator: Davidson and Missouri Western
created: 2006-07-31T11:00:00Z
modified: 2015-08-31T04:08:45Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _61_
m user id: 0
owner id: 919
owning group id: 61
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part contains the coding region for tetracylcine resistance flanked by Hix C sites. This part is going to be used to test the optimal location of the RBS. If the Hin(BBa_J31000) or Hin LVA (BBa_J31001) genes are placed upstream of this part there is a possibility that the coding region between the Hix C sites is inverted.
mutable notes: This part is going to be used to test where the RBS needs to be placed.
mutable provenance: None
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_J31007
component: BBa_B0012
component: BBa_J44000
component: BBa_B0010
component: BBa_I13453
component: BBa_B0030
component: BBa_J3101
sequence annotation: BBa_B0012
sequence annotation: BBa_B0030
sequence annotation: BBa_J44000
sequence annotation: BBa_J3101
sequence annotation: BBa_J31007
sequence annotation: BBa_I13453
sequence annotation: BBa_B0010
sequence: https://synbiohub.org/public/igem/BBa_J3103_sequence/1

BBa_J31007
type: Component
persistent identity: component1892401
display id: component1892401
version: 1
top level: BBa_J3103
access: public
definition: https://synbiohub.org/public/igem/BBa_J31007/1

BBa_B0012
type: Component
persistent identity: component1892405
display id: component1892405
version: 1
top level: BBa_J3103
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_J44000
type: Component
persistent identity: component1892396
display id: component1892396
version: 1
top level: BBa_J3103
access: public
definition: hixC

BBa_B0010
type: Component
persistent identity: component1892403
display id: component1892403
version: 1
top level: BBa_J3103
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_I13453
type: Component
persistent identity: component1892395
display id: component1892395
version: 1
top level: BBa_J3103
access: public
definition: https://synbiohub.org/public/igem/BBa_I13453/1

BBa_B0030
type: Component
persistent identity: component1892398
display id: component1892398
version: 1
top level: BBa_J3103
access: public
definition: https://synbiohub.org/public/igem/BBa_B0030/1

BBa_J44000
type: Component
persistent identity: component1892402
display id: component1892402
version: 1
top level: BBa_J3103
access: public
definition: hixC

BBa_J3101
type: Component
persistent identity: component1892415
display id: component1892415
version: 1
top level: BBa_J3103
access: public
definition: RE

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1892405
display id: annotation1892405
version: 1
top level: BBa_J3103
location: https://synbiohub.org/public/igem/BBa_J3103/annotation1892405/range1892405/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_J3103/annotation1892405/range1892405/1
type: Range
persistent identity: range1892405
display id: range1892405
version: 1
direction: direction/forward
top level: BBa_J3103
start: 1515
end: 1555
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation1892398
display id: annotation1892398
version: 1
top level: BBa_J3103
location: https://synbiohub.org/public/igem/BBa_J3103/annotation1892398/range1892398/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_J3103/annotation1892398/range1892398/1
type: Range
persistent identity: range1892398
display id: range1892398
version: 1
direction: direction/forward
top level: BBa_J3103
start: 173
end: 187
orientation: inline

BBa_J44000
type: SequenceAnnotation
persistent identity: annotation1892402
display id: annotation1892402
version: 1
top level: BBa_J3103
location: https://synbiohub.org/public/igem/BBa_J3103/annotation1892402/range1892402/1
component: BBa_J44000

https://synbiohub.org/public/igem/BBa_J3103/annotation1892402/range1892402/1
type: Range
persistent identity: range1892402
display id: range1892402
version: 1
direction: direction/reverse
top level: BBa_J3103
start: 1393
end: 1418
orientation: inline

BBa_J3101
type: SequenceAnnotation
persistent identity: annotation1892415
display id: annotation1892415
version: 1
top level: BBa_J3103
location: https://synbiohub.org/public/igem/BBa_J3103/annotation1892415/range1892415/1
component: BBa_J3101

https://synbiohub.org/public/igem/BBa_J3103/annotation1892415/range1892415/1
type: Range
persistent identity: range1892415
display id: range1892415
version: 1
direction: direction/reverse
top level: BBa_J3103
start: 1564
end: 1640
orientation: inline

BBa_J31007
type: SequenceAnnotation
persistent identity: annotation1892401
display id: annotation1892401
version: 1
top level: BBa_J3103
location: https://synbiohub.org/public/igem/BBa_J3103/annotation1892401/range1892401/1
component: BBa_J31007

https://synbiohub.org/public/igem/BBa_J3103/annotation1892401/range1892401/1
type: Range
persistent identity: range1892401
display id: range1892401
version: 1
direction: direction/reverse
top level: BBa_J3103
start: 194
end: 1384
orientation: inline

BBa_J44000
type: SequenceAnnotation
persistent identity: annotation1892396
display id: annotation1892396
version: 1
top level: BBa_J3103
location: https://synbiohub.org/public/igem/BBa_J3103/annotation1892396/range1892396/1
component: BBa_J44000

https://synbiohub.org/public/igem/BBa_J3103/annotation1892396/range1892396/1
type: Range
persistent identity: range1892396
display id: range1892396
version: 1
direction: direction/reverse
top level: BBa_J3103
start: 139
end: 164
orientation: inline

BBa_I13453
type: SequenceAnnotation
persistent identity: annotation1892395
display id: annotation1892395
version: 1
top level: BBa_J3103
location: https://synbiohub.org/public/igem/BBa_J3103/annotation1892395/range1892395/1
component: BBa_I13453

https://synbiohub.org/public/igem/BBa_J3103/annotation1892395/range1892395/1
type: Range
persistent identity: range1892395
display id: range1892395
version: 1
direction: direction/reverse
top level: BBa_J3103
start: 1
end: 130
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1892403
display id: annotation1892403
version: 1
top level: BBa_J3103
location: https://synbiohub.org/public/igem/BBa_J3103/annotation1892403/range1892403/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_J3103/annotation1892403/range1892403/1
type: Range
persistent identity: range1892403
display id: range1892403
version: 1
direction: direction/forward
top level: BBa_J3103
start: 1427
end: 1506
orientation: inline

BBa_I715044
type: ComponentDefinition
persistent identity: BBa I715044
display id: BBa_I715044
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: HPP-A2
creator: Tom Crowley
created: 2007-08-22T11:00:00Z
modified: 2015-08-31T04:07:49Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _120_
m user id: 0
owner id: 1803
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: T7prom-RBS-RFP1-hixC-GFP2-TT-hixC-RFP2-RBS-GFP1-hixC-RFP2-TT-hixC
mutable notes: None
mutable provenance: Built from construction intermediates
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_I715023
component: BBa_J44000
component: BBa_B0012
component: BBa_I715020
component: BBa_R0085
component: BBa_I715022
component: BBa_B0034
component: BBa_B0010
component: BBa_I715019
sequence annotation: BBa_I715022
sequence annotation: BBa_R0085
sequence annotation: BBa_I715019
sequence annotation: BBa_B0012
sequence annotation: BBa_B0010
sequence annotation: BBa_J44000
sequence annotation: BBa_B0034
sequence annotation: BBa_I715023
sequence annotation: BBa_I715020
sequence: https://synbiohub.org/public/igem/BBa_I715044_sequence/1

BBa_I715023
type: Component
persistent identity: component1942436
display id: component1942436
version: 1
top level: BBa_I715044
access: public
definition: https://synbiohub.org/public/igem/BBa_I715023/1

BBa_J44000
type: Component
persistent identity: component1942414
display id: component1942414
version: 1
top level: BBa_I715044
access: public
definition: hixC

BBa_I715023
type: Component
persistent identity: component1942427
display id: component1942427
version: 1
top level: BBa_I715044
access: public
definition: https://synbiohub.org/public/igem/BBa_I715023/1

BBa_B0012
type: Component
persistent identity: component1942420
display id: component1942420
version: 1
top level: BBa_I715044
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_I715020
type: Component
persistent identity: component1942417
display id: component1942417
version: 1
top level: BBa_I715044
access: public
definition: https://synbiohub.org/public/igem/BBa_I715020/1

BBa_R0085
type: Component
persistent identity: component1942408
display id: component1942408
version: 1
top level: BBa_I715044
access: public
definition: https://synbiohub.org/public/igem/BBa_R0085/1

BBa_J44000
type: Component
persistent identity: component1942443
display id: component1942443
version: 1
top level: BBa_I715044
access: public
definition: hixC

BBa_I715022
type: Component
persistent identity: component1942413
display id: component1942413
version: 1
top level: BBa_I715044
access: public
definition: https://synbiohub.org/public/igem/BBa_I715022/1

BBa_B0034
type: Component
persistent identity: component1942410
display id: component1942410
version: 1
top level: BBa_I715044
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0010
type: Component
persistent identity: component1942437
display id: component1942437
version: 1
top level: BBa_I715044
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_J44000
type: Component
persistent identity: component1942433
display id: component1942433
version: 1
top level: BBa_I715044
access: public
definition: hixC

BBa_B0012
type: Component
persistent identity: component1942439
display id: component1942439
version: 1
top level: BBa_I715044
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_I715019
type: Component
persistent identity: component1942432
display id: component1942432
version: 1
top level: BBa_I715044
access: public
definition: https://synbiohub.org/public/igem/BBa_I715019/1

BBa_B0034
type: Component
persistent identity: component1942429
display id: component1942429
version: 1
top level: BBa_I715044
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_J44000
type: Component
persistent identity: component1942424
display id: component1942424
version: 1
top level: BBa_I715044
access: public
definition: hixC

BBa_B0010
type: Component
persistent identity: component1942418
display id: component1942418
version: 1
top level: BBa_I715044
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_I715022
type: SequenceAnnotation
persistent identity: annotation1942413
display id: annotation1942413
version: 1
top level: BBa_I715044
location: https://synbiohub.org/public/igem/BBa_I715044/annotation1942413/range1942413/1
component: BBa_I715022

https://synbiohub.org/public/igem/BBa_I715044/annotation1942413/range1942413/1
type: Range
persistent identity: range1942413
display id: range1942413
version: 1
direction: direction/reverse
top level: BBa_I715044
start: 50
end: 511
orientation: inline

BBa_R0085
type: SequenceAnnotation
persistent identity: annotation1942408
display id: annotation1942408
version: 1
top level: BBa_I715044
location: https://synbiohub.org/public/igem/BBa_I715044/annotation1942408/range1942408/1
component: BBa_R0085

https://synbiohub.org/public/igem/BBa_I715044/annotation1942408/range1942408/1
type: Range
persistent identity: range1942408
display id: range1942408
version: 1
direction: direction/reverse
top level: BBa_I715044
start: 1
end: 23
orientation: inline

BBa_I715019
type: SequenceAnnotation
persistent identity: annotation1942432
display id: annotation1942432
version: 1
top level: BBa_I715044
location: https://synbiohub.org/public/igem/BBa_I715044/annotation1942432/range1942432/1
component: BBa_I715019

https://synbiohub.org/public/igem/BBa_I715044/annotation1942432/range1942432/1
type: Range
persistent identity: range1942432
display id: range1942432
version: 1
direction: direction/reverse
top level: BBa_I715044
start: 1229
end: 1699
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1942420
display id: annotation1942420
version: 1
top level: BBa_I715044
location: https://synbiohub.org/public/igem/BBa_I715044/annotation1942420/range1942420/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I715044/annotation1942420/range1942420/1
type: Range
persistent identity: range1942420
display id: range1942420
version: 1
direction: direction/forward
top level: BBa_I715044
start: 900
end: 940
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1942437
display id: annotation1942437
version: 1
top level: BBa_I715044
location: https://synbiohub.org/public/igem/BBa_I715044/annotation1942437/range1942437/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I715044/annotation1942437/range1942437/1
type: Range
persistent identity: range1942437
display id: range1942437
version: 1
direction: direction/forward
top level: BBa_I715044
start: 1970
end: 2049
orientation: inline

BBa_J44000
type: SequenceAnnotation
persistent identity: annotation1942424
display id: annotation1942424
version: 1
top level: BBa_I715044
location: https://synbiohub.org/public/igem/BBa_I715044/annotation1942424/range1942424/1
component: BBa_J44000

https://synbiohub.org/public/igem/BBa_I715044/annotation1942424/range1942424/1
type: Range
persistent identity: range1942424
display id: range1942424
version: 1
direction: direction/reverse
top level: BBa_I715044
start: 949
end: 974
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1942439
display id: annotation1942439
version: 1
top level: BBa_I715044
location: https://synbiohub.org/public/igem/BBa_I715044/annotation1942439/range1942439/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I715044/annotation1942439/range1942439/1
type: Range
persistent identity: range1942439
display id: range1942439
version: 1
direction: direction/forward
top level: BBa_I715044
start: 2058
end: 2098
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1942418
display id: annotation1942418
version: 1
top level: BBa_I715044
location: https://synbiohub.org/public/igem/BBa_I715044/annotation1942418/range1942418/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I715044/annotation1942418/range1942418/1
type: Range
persistent identity: range1942418
display id: range1942418
version: 1
direction: direction/forward
top level: BBa_I715044
start: 812
end: 891
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1942429
display id: annotation1942429
version: 1
top level: BBa_I715044
location: https://synbiohub.org/public/igem/BBa_I715044/annotation1942429/range1942429/1
component: BBa_B0034

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type: Range
persistent identity: range1942429
display id: range1942429
version: 1
direction: direction/reverse
top level: BBa_I715044
start: 1211
end: 1222
orientation: inline

BBa_I715023
type: SequenceAnnotation
persistent identity: annotation1942436
display id: annotation1942436
version: 1
top level: BBa_I715044
location: https://synbiohub.org/public/igem/BBa_I715044/annotation1942436/range1942436/1
component: BBa_I715023

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type: Range
persistent identity: range1942436
display id: range1942436
version: 1
direction: direction/reverse
top level: BBa_I715044
start: 1742
end: 1961
orientation: inline

BBa_J44000
type: SequenceAnnotation
persistent identity: annotation1942443
display id: annotation1942443
version: 1
top level: BBa_I715044
location: https://synbiohub.org/public/igem/BBa_I715044/annotation1942443/range1942443/1
component: BBa_J44000

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type: Range
persistent identity: range1942443
display id: range1942443
version: 1
direction: direction/reverse
top level: BBa_I715044
start: 2107
end: 2132
orientation: inline

BBa_I715023
type: SequenceAnnotation
persistent identity: annotation1942427
display id: annotation1942427
version: 1
top level: BBa_I715044
location: https://synbiohub.org/public/igem/BBa_I715044/annotation1942427/range1942427/1
component: BBa_I715023

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type: Range
persistent identity: range1942427
display id: range1942427
version: 1
direction: direction/reverse
top level: BBa_I715044
start: 983
end: 1202
orientation: inline

BBa_J44000
type: SequenceAnnotation
persistent identity: annotation1942433
display id: annotation1942433
version: 1
top level: BBa_I715044
location: https://synbiohub.org/public/igem/BBa_I715044/annotation1942433/range1942433/1
component: BBa_J44000

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type: Range
persistent identity: range1942433
display id: range1942433
version: 1
direction: direction/reverse
top level: BBa_I715044
start: 1708
end: 1733
orientation: inline

BBa_J44000
type: SequenceAnnotation
persistent identity: annotation1942414
display id: annotation1942414
version: 1
top level: BBa_I715044
location: https://synbiohub.org/public/igem/BBa_I715044/annotation1942414/range1942414/1
component: BBa_J44000

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type: Range
persistent identity: range1942414
display id: range1942414
version: 1
direction: direction/reverse
top level: BBa_I715044
start: 520
end: 545
orientation: inline

BBa_I715020
type: SequenceAnnotation
persistent identity: annotation1942417
display id: annotation1942417
version: 1
top level: BBa_I715044
location: https://synbiohub.org/public/igem/BBa_I715044/annotation1942417/range1942417/1
component: BBa_I715020

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type: Range
persistent identity: range1942417
display id: range1942417
version: 1
direction: direction/reverse
top level: BBa_I715044
start: 554
end: 803
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1942410
display id: annotation1942410
version: 1
top level: BBa_I715044
location: https://synbiohub.org/public/igem/BBa_I715044/annotation1942410/range1942410/1
component: BBa_B0034

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type: Range
persistent identity: range1942410
display id: range1942410
version: 1
direction: direction/reverse
top level: BBa_I715044
start: 32
end: 43
orientation: inline

BBa_J3108
type: ComponentDefinition
persistent identity: BBa J3108
display id: BBa_J3108
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: AraC-PC, pLac-HinLVA
creator: Karmella Haynes
created: 2006-10-11T11:00:00Z
modified: 2015-08-31T04:08:45Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _61_
m user id: 0
owner id: 1144
owning group id: 61
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Should constitutively express the AraC protein (pBad inhibitor). HinLVA is under the control of pLac. Therefore, HinLVA should be expressed in the presence of IPTG.
mutable notes: The double forward terminator is placed uptream of pLac-HinLVA to prevent forward read-through transcription from the pSB1AK3 vector.
mutable provenance: AraC-PC-TT - part BBa_S03591 pLac-HinLVA - part BBa_S03536
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0010
component: BBa_B0012
component: BBa_I13458
component: BBa_R0010
component: BBa_J31001
component: BBa_B0030
sequence annotation: BBa_R0010
sequence annotation: BBa_B0012
sequence annotation: BBa_B0030
sequence annotation: BBa_J31001
sequence annotation: BBa_I13458
sequence annotation: BBa_B0010
sequence: https://synbiohub.org/public/igem/BBa_J3108_sequence/1

BBa_B0010
type: Component
persistent identity: component1902785
display id: component1902785
version: 1
top level: BBa_J3108
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0012
type: Component
persistent identity: component1902787
display id: component1902787
version: 1
top level: BBa_J3108
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_I13458
type: Component
persistent identity: component1902784
display id: component1902784
version: 1
top level: BBa_J3108
access: public
definition: https://synbiohub.org/public/igem/BBa_I13458/1

BBa_B0012
type: Component
persistent identity: component1902807
display id: component1902807
version: 1
top level: BBa_J3108
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_R0010
type: Component
persistent identity: component1902791
display id: component1902791
version: 1
top level: BBa_J3108
access: public
definition: https://synbiohub.org/public/igem/BBa_R0010/1

BBa_J31001
type: Component
persistent identity: component1902804
display id: component1902804
version: 1
top level: BBa_J3108
access: public
definition: Hin LVA

BBa_B0010
type: Component
persistent identity: component1902805
display id: component1902805
version: 1
top level: BBa_J3108
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0030
type: Component
persistent identity: component1902799
display id: component1902799
version: 1
top level: BBa_J3108
access: public
definition: https://synbiohub.org/public/igem/BBa_B0030/1

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation1902791
display id: annotation1902791
version: 1
top level: BBa_J3108
location: https://synbiohub.org/public/igem/BBa_J3108/annotation1902791/range1902791/1
component: BBa_R0010

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type: Range
persistent identity: range1902791
display id: range1902791
version: 1
direction: direction/forward
top level: BBa_J3108
start: 1260
end: 1459
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1902787
display id: annotation1902787
version: 1
top level: BBa_J3108
location: https://synbiohub.org/public/igem/BBa_J3108/annotation1902787/range1902787/1
component: BBa_B0012

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type: Range
persistent identity: range1902787
display id: range1902787
version: 1
direction: direction/forward
top level: BBa_J3108
start: 1211
end: 1251
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1902807
display id: annotation1902807
version: 1
top level: BBa_J3108
location: https://synbiohub.org/public/igem/BBa_J3108/annotation1902807/range1902807/1
component: BBa_B0012

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type: Range
persistent identity: range1902807
display id: range1902807
version: 1
direction: direction/forward
top level: BBa_J3108
start: 2197
end: 2237
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation1902799
display id: annotation1902799
version: 1
top level: BBa_J3108
location: https://synbiohub.org/public/igem/BBa_J3108/annotation1902799/range1902799/1
component: BBa_B0030

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type: Range
persistent identity: range1902799
display id: range1902799
version: 1
direction: direction/forward
top level: BBa_J3108
start: 1468
end: 1482
orientation: inline

BBa_J31001
type: SequenceAnnotation
persistent identity: annotation1902804
display id: annotation1902804
version: 1
top level: BBa_J3108
location: https://synbiohub.org/public/igem/BBa_J3108/annotation1902804/range1902804/1
component: BBa_J31001

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type: Range
persistent identity: range1902804
display id: range1902804
version: 1
direction: direction/reverse
top level: BBa_J3108
start: 1489
end: 2100
orientation: inline

BBa_I13458
type: SequenceAnnotation
persistent identity: annotation1902784
display id: annotation1902784
version: 1
top level: BBa_J3108
location: https://synbiohub.org/public/igem/BBa_J3108/annotation1902784/range1902784/1
component: BBa_I13458

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type: Range
persistent identity: range1902784
display id: range1902784
version: 1
direction: direction/reverse
top level: BBa_J3108
start: 1
end: 1114
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1902805
display id: annotation1902805
version: 1
top level: BBa_J3108
location: https://synbiohub.org/public/igem/BBa_J3108/annotation1902805/range1902805/1
component: BBa_B0010

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type: Range
persistent identity: range1902805
display id: range1902805
version: 1
direction: direction/forward
top level: BBa_J3108
start: 2109
end: 2188
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1902785
display id: annotation1902785
version: 1
top level: BBa_J3108
location: https://synbiohub.org/public/igem/BBa_J3108/annotation1902785/range1902785/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_J3108/annotation1902785/range1902785/1
type: Range
persistent identity: range1902785
display id: range1902785
version: 1
direction: direction/forward
top level: BBa_J3108
start: 1123
end: 1202
orientation: inline

Hin LVA
type: ComponentDefinition
persistent identity: BBa J31001
display id: BBa_J31001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: DNA invertase Hin tagged with LVA
creator: Erin Zwack, Sabriya Rosemond
created: 2006-07-11T11:00:00Z
modified: 2015-08-31T04:08:45Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _61_
m user id: 0
owner id: 918
owning group id: 61
sample status: In stock
status: status/Available
bookmark: true
mutable description: This part produces the Hin protein with an LVA degredation tag. We are currently looking into this as a way to ensure no Hin is floating around once we turn off the Hin promoter.
mutable notes: None really
mutable provenance: Salmonella typhimurium and the the Hin part without LVA.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Hin
sequence annotation: Rev primer J31001
sequence annotation: LVA
sequence annotation: Fwd primer J31001
sequence annotation: Stop codon
sequence: https://synbiohub.org/public/igem/BBa_J31001_sequence/1

Hin
type: SequenceAnnotation
persistent identity: annotation1910564
display id: annotation1910564
version: 1
top level: Hin LVA
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J31001/annotation1910564/range1910564/1

https://synbiohub.org/public/igem/BBa_J31001/annotation1910564/range1910564/1
type: Range
persistent identity: range1910564
display id: range1910564
version: 1
direction: direction/forward
top level: Hin LVA
start: 1
end: 570
orientation: inline

Rev primer J31001
type: SequenceAnnotation
persistent identity: annotation1910570
display id: annotation1910570
version: 1
top level: Hin LVA
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_J31001/annotation1910570/range1910570/1

https://synbiohub.org/public/igem/BBa_J31001/annotation1910570/range1910570/1
type: Range
persistent identity: range1910570
display id: range1910570
version: 1
direction: direction/reverse
top level: Hin LVA
start: 551
end: 612
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation1910566
display id: annotation1910566
version: 1
top level: Hin LVA
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_J31001/annotation1910566/range1910566/1

https://synbiohub.org/public/igem/BBa_J31001/annotation1910566/range1910566/1
type: Range
persistent identity: range1910566
display id: range1910566
version: 1
direction: direction/forward
top level: Hin LVA
start: 571
end: 609
orientation: inline

Fwd primer J31001
type: SequenceAnnotation
persistent identity: annotation1910565
display id: annotation1910565
version: 1
top level: Hin LVA
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_J31001/annotation1910565/range1910565/1

https://synbiohub.org/public/igem/BBa_J31001/annotation1910565/range1910565/1
type: Range
persistent identity: range1910565
display id: range1910565
version: 1
direction: direction/forward
top level: Hin LVA
start: 1
end: 3
orientation: inline

Stop codon
type: SequenceAnnotation
persistent identity: annotation1910567
display id: annotation1910567
version: 1
top level: Hin LVA
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_J31001/annotation1910567/range1910567/1

https://synbiohub.org/public/igem/BBa_J31001/annotation1910567/range1910567/1
type: Range
persistent identity: range1910567
display id: range1910567
version: 1
direction: direction/forward
top level: Hin LVA
start: 610
end: 612
orientation: inline

BBa_J3104
type: ComponentDefinition
persistent identity: BBa J3104
display id: BBa_J3104
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pBad-RBS-HixC-TetF-HixC-TT-RE
creator: Davidson and Missouri Western
created: 2006-07-31T11:00:00Z
modified: 2015-08-31T04:08:45Z
discontinued: false
dominant: false
experience: experience/Fails
group u list: _61_
m user id: 0
owner id: 919
owning group id: 61
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part contains the gene for tetracycline resistance flanked by HixC regions. This part will be used to test if the ribosome will be able to read through the Hix site.
mutable notes: This part as well as part BBa_J3103 are going to be used to find the optimal placement of the RBS.
mutable provenance: None
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_J3101
component: BBa_J44000
component: BBa_B0030
component: BBa_I13453
component: BBa_B0010
component: BBa_J31007
component: BBa_B0012
sequence annotation: BBa_J44000
sequence annotation: BBa_J3101
sequence annotation: BBa_B0012
sequence annotation: BBa_B0030
sequence annotation: BBa_J31007
sequence annotation: BBa_B0010
sequence annotation: BBa_I13453
sequence: https://synbiohub.org/public/igem/BBa_J3104_sequence/1

BBa_J3101
type: Component
persistent identity: component1892436
display id: component1892436
version: 1
top level: BBa_J3104
access: public
definition: RE

BBa_J44000
type: Component
persistent identity: component1892420
display id: component1892420
version: 1
top level: BBa_J3104
access: public
definition: hixC

BBa_B0030
type: Component
persistent identity: component1892418
display id: component1892418
version: 1
top level: BBa_J3104
access: public
definition: https://synbiohub.org/public/igem/BBa_B0030/1

BBa_I13453
type: Component
persistent identity: component1892416
display id: component1892416
version: 1
top level: BBa_J3104
access: public
definition: https://synbiohub.org/public/igem/BBa_I13453/1

BBa_J44000
type: Component
persistent identity: component1892423
display id: component1892423
version: 1
top level: BBa_J3104
access: public
definition: hixC

BBa_B0010
type: Component
persistent identity: component1892424
display id: component1892424
version: 1
top level: BBa_J3104
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_J31007
type: Component
persistent identity: component1892422
display id: component1892422
version: 1
top level: BBa_J3104
access: public
definition: https://synbiohub.org/public/igem/BBa_J31007/1

BBa_B0012
type: Component
persistent identity: component1892426
display id: component1892426
version: 1
top level: BBa_J3104
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_J44000
type: SequenceAnnotation
persistent identity: annotation1892420
display id: annotation1892420
version: 1
top level: BBa_J3104
location: https://synbiohub.org/public/igem/BBa_J3104/annotation1892420/range1892420/1
component: BBa_J44000

https://synbiohub.org/public/igem/BBa_J3104/annotation1892420/range1892420/1
type: Range
persistent identity: range1892420
display id: range1892420
version: 1
direction: direction/reverse
top level: BBa_J3104
start: 162
end: 187
orientation: inline

BBa_J3101
type: SequenceAnnotation
persistent identity: annotation1892436
display id: annotation1892436
version: 1
top level: BBa_J3104
location: https://synbiohub.org/public/igem/BBa_J3104/annotation1892436/range1892436/1
component: BBa_J3101

https://synbiohub.org/public/igem/BBa_J3104/annotation1892436/range1892436/1
type: Range
persistent identity: range1892436
display id: range1892436
version: 1
direction: direction/reverse
top level: BBa_J3104
start: 1564
end: 1640
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1892426
display id: annotation1892426
version: 1
top level: BBa_J3104
location: https://synbiohub.org/public/igem/BBa_J3104/annotation1892426/range1892426/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_J3104/annotation1892426/range1892426/1
type: Range
persistent identity: range1892426
display id: range1892426
version: 1
direction: direction/forward
top level: BBa_J3104
start: 1515
end: 1555
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation1892418
display id: annotation1892418
version: 1
top level: BBa_J3104
location: https://synbiohub.org/public/igem/BBa_J3104/annotation1892418/range1892418/1
component: BBa_B0030

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type: Range
persistent identity: range1892418
display id: range1892418
version: 1
direction: direction/forward
top level: BBa_J3104
start: 139
end: 153
orientation: inline

BBa_J31007
type: SequenceAnnotation
persistent identity: annotation1892422
display id: annotation1892422
version: 1
top level: BBa_J3104
location: https://synbiohub.org/public/igem/BBa_J3104/annotation1892422/range1892422/1
component: BBa_J31007

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type: Range
persistent identity: range1892422
display id: range1892422
version: 1
direction: direction/reverse
top level: BBa_J3104
start: 194
end: 1384
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1892424
display id: annotation1892424
version: 1
top level: BBa_J3104
location: https://synbiohub.org/public/igem/BBa_J3104/annotation1892424/range1892424/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_J3104/annotation1892424/range1892424/1
type: Range
persistent identity: range1892424
display id: range1892424
version: 1
direction: direction/forward
top level: BBa_J3104
start: 1427
end: 1506
orientation: inline

BBa_I13453
type: SequenceAnnotation
persistent identity: annotation1892416
display id: annotation1892416
version: 1
top level: BBa_J3104
location: https://synbiohub.org/public/igem/BBa_J3104/annotation1892416/range1892416/1
component: BBa_I13453

https://synbiohub.org/public/igem/BBa_J3104/annotation1892416/range1892416/1
type: Range
persistent identity: range1892416
display id: range1892416
version: 1
direction: direction/reverse
top level: BBa_J3104
start: 1
end: 130
orientation: inline

BBa_J44000
type: SequenceAnnotation
persistent identity: annotation1892423
display id: annotation1892423
version: 1
top level: BBa_J3104
location: https://synbiohub.org/public/igem/BBa_J3104/annotation1892423/range1892423/1
component: BBa_J44000

https://synbiohub.org/public/igem/BBa_J3104/annotation1892423/range1892423/1
type: Range
persistent identity: range1892423
display id: range1892423
version: 1
direction: direction/reverse
top level: BBa_J3104
start: 1393
end: 1418
orientation: inline

BBa_J3106
type: ComponentDefinition
persistent identity: BBa J3106
display id: BBa_J3106
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pBad-HixC-TetB-RBSrev-HixC-TT-RE
creator: Davidson and Missouri Western
created: 2006-08-01T11:00:00Z
modified: 2015-08-31T04:08:45Z
discontinued: false
dominant: false
experience: experience/Issues
group u list: _61_
m user id: 0
owner id: 919
owning group id: 61
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part contains the tetracycline resistance in the backwards orientation as well as a backwards RBS. These parts are flanked by HixC sites so when this part is placed downstream of the Hin or HinLVA cassettes the RBS and the tetracycline resistance will be in the correct orientation and the cell will express tetracyline resistance.
mutable notes: We needed to create a backwards RBS.
mutable provenance: none
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_S03533
component: BBa_S03516
sequence annotation: BBa_S03516
sequence annotation: BBa_S03533
sequence: https://synbiohub.org/public/igem/BBa_J3106_sequence/1

BBa_S03533
type: Component
persistent identity: component2224989
display id: component2224989
version: 1
top level: BBa_J3106
access: public
definition: https://synbiohub.org/public/igem/BBa_S03533/1

BBa_S03516
type: Component
persistent identity: component2225003
display id: component2225003
version: 1
top level: BBa_J3106
access: public
definition: https://synbiohub.org/public/igem/BBa_S03516/1

BBa_S03516
type: SequenceAnnotation
persistent identity: annotation2225003
display id: annotation2225003
version: 1
top level: BBa_J3106
location: https://synbiohub.org/public/igem/BBa_J3106/annotation2225003/range2225003/1
component: BBa_S03516

https://synbiohub.org/public/igem/BBa_J3106/annotation2225003/range2225003/1
type: Range
persistent identity: range2225003
display id: range2225003
version: 1
direction: direction/reverse
top level: BBa_J3106
start: 1395
end: 1608
orientation: inline

BBa_S03533
type: SequenceAnnotation
persistent identity: annotation2224989
display id: annotation2224989
version: 1
top level: BBa_J3106
location: https://synbiohub.org/public/igem/BBa_J3106/annotation2224989/range2224989/1
component: BBa_S03533

https://synbiohub.org/public/igem/BBa_J3106/annotation2224989/range2224989/1
type: Range
persistent identity: range2224989
display id: range2224989
version: 1
direction: direction/reverse
top level: BBa_J3106
start: 1
end: 1386
orientation: inline

BBa_J3105
type: ComponentDefinition
persistent identity: BBa J3105
display id: BBa_J3105
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pBad-RBS-HixC-TetB-HixC-TT-RE
creator: Davidson and Missouri Western
created: 2006-07-31T11:00:00Z
modified: 2015-08-31T04:08:45Z
discontinued: false
dominant: false
experience: experience/Issues
group u list: _61_
m user id: 0
owner id: 919
owning group id: 61
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part contains the backwards coding region for the tetracycline resistance gene flanked by Hix C sites. If the Hin( BBaJ31000) ot the Hin-LVA (BBa_J31001) gene with a promoter and terminator, the backwards TetR gene will be inverted so that tetracyline resistance will be expressed.
mutable notes: None
mutable provenance: None
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_J3102
component: BBa_S03528
sequence annotation: BBa_J3102
sequence annotation: BBa_S03528
sequence: https://synbiohub.org/public/igem/BBa_J3105_sequence/1

BBa_J3102
type: Component
persistent identity: component2226272
display id: component2226272
version: 1
top level: BBa_J3105
access: public
definition: https://synbiohub.org/public/igem/BBa_J3102/1

BBa_S03528
type: Component
persistent identity: component2226296
display id: component2226296
version: 1
top level: BBa_J3105
access: public
definition: https://synbiohub.org/public/igem/BBa_S03528/1

BBa_J3102
type: SequenceAnnotation
persistent identity: annotation2226272
display id: annotation2226272
version: 1
top level: BBa_J3105
location: https://synbiohub.org/public/igem/BBa_J3105/annotation2226272/range2226272/1
component: BBa_J3102

https://synbiohub.org/public/igem/BBa_J3105/annotation2226272/range2226272/1
type: Range
persistent identity: range2226272
display id: range2226272
version: 1
direction: direction/reverse
top level: BBa_J3105
start: 1
end: 153
orientation: inline

BBa_S03528
type: SequenceAnnotation
persistent identity: annotation2226296
display id: annotation2226296
version: 1
top level: BBa_J3105
location: https://synbiohub.org/public/igem/BBa_J3105/annotation2226296/range2226296/1
component: BBa_S03528

https://synbiohub.org/public/igem/BBa_J3105/annotation2226296/range2226296/1
type: Range
persistent identity: range2226296
display id: range2226296
version: 1
direction: direction/reverse
top level: BBa_J3105
start: 162
end: 1642
orientation: inline

Hin
type: ComponentDefinition
persistent identity: BBa J31000
display id: BBa_J31000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: DNA-invertase Hin from Salmonella typhimurium
creator: Erin Zwack, Sabriya Rosemond
created: 2006-06-04T11:00:00Z
modified: 2015-08-31T04:08:45Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _61_
m user id: 0
owner id: 918
owning group id: 61
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Generates the Salmonella typhimurium Hin protein. This protein is used to invert DNA that is flanked by Hix siteds.
mutable notes: none really
mutable provenance: Salmonella typhimurium
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Former SpeI site
sequence annotation: Rev primer J31001
sequence annotation: Fwd primer J31001
sequence annotation: Mutation to delete the SpeI site
sequence annotation: Hin sequence
sequence: https://synbiohub.org/public/igem/BBa_J31000_sequence/1

Former SpeI site
type: SequenceAnnotation
persistent identity: annotation1880459
display id: annotation1880459
version: 1
top level: Hin
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_J31000/annotation1880459/range1880459/1

https://synbiohub.org/public/igem/BBa_J31000/annotation1880459/range1880459/1
type: Range
persistent identity: range1880459
display id: range1880459
version: 1
direction: direction/forward
top level: Hin
start: 70
end: 75
orientation: inline

Rev primer J31001
type: SequenceAnnotation
persistent identity: annotation1910569
display id: annotation1910569
version: 1
top level: Hin
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_J31000/annotation1910569/range1910569/1

https://synbiohub.org/public/igem/BBa_J31000/annotation1910569/range1910569/1
type: Range
persistent identity: range1910569
display id: range1910569
version: 1
direction: direction/reverse
top level: Hin
start: 551
end: 570
orientation: inline

Fwd primer J31001
type: SequenceAnnotation
persistent identity: annotation1910568
display id: annotation1910568
version: 1
top level: Hin
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_J31000/annotation1910568/range1910568/1

https://synbiohub.org/public/igem/BBa_J31000/annotation1910568/range1910568/1
type: Range
persistent identity: range1910568
display id: range1910568
version: 1
direction: direction/forward
top level: Hin
start: 1
end: 3
orientation: inline

Mutation to delete the SpeI site
type: SequenceAnnotation
persistent identity: annotation1880460
display id: annotation1880460
version: 1
top level: Hin
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_J31000/annotation1880460/range1880460/1

https://synbiohub.org/public/igem/BBa_J31000/annotation1880460/range1880460/1
type: Range
persistent identity: range1880460
display id: range1880460
version: 1
direction: direction/forward
top level: Hin
start: 72
end: 72
orientation: inline

Hin sequence
type: SequenceAnnotation
persistent identity: annotation1884987
display id: annotation1884987
version: 1
top level: Hin
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J31000/annotation1884987/range1884987/1

https://synbiohub.org/public/igem/BBa_J31000/annotation1884987/range1884987/1
type: Range
persistent identity: range1884987
display id: range1884987
version: 1
direction: direction/forward
top level: Hin
start: 1
end: 573
orientation: inline

BBa_I715037
type: ComponentDefinition
persistent identity: BBa I715037
display id: BBa_I715037
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: HPP-B1
creator: Andrew Martens
created: 2007-07-28T11:00:00Z
modified: 2015-08-31T04:07:49Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _120_
m user id: 0
owner id: 1620
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This is the first computer that we will build. In its starting orientation there are no phenotypes expressed. There is the possibility of a false positive. There is only one true positive.
mutable notes: Alternatively, we may try building it in fewer steps doing a triple ligation.
mutable provenance: Combine parts BBa_S03763 and BBa_S03764.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_J44000
component: BBa_B0012
component: BBa_B0034
component: BBa_I715022
component: BBa_I715020
component: BBa_I715019
component: BBa_I715023
component: BBa_B0010
component: BBa_R0085
sequence annotation: BBa_J44000
sequence annotation: BBa_B0034
sequence annotation: BBa_I715020
sequence annotation: BBa_I715023
sequence annotation: BBa_B0012
sequence annotation: BBa_I715022
sequence annotation: BBa_R0085
sequence annotation: BBa_B0010
sequence annotation: BBa_I715019
sequence: https://synbiohub.org/public/igem/BBa_I715037_sequence/1

BBa_J44000
type: Component
persistent identity: component1940151
display id: component1940151
version: 1
top level: BBa_I715037
access: public
definition: hixC

BBa_B0012
type: Component
persistent identity: component1940147
display id: component1940147
version: 1
top level: BBa_I715037
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0034
type: Component
persistent identity: component1940156
display id: component1940156
version: 1
top level: BBa_I715037
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_I715022
type: Component
persistent identity: component1940140
display id: component1940140
version: 1
top level: BBa_I715037
access: public
definition: https://synbiohub.org/public/igem/BBa_I715022/1

BBa_I715020
type: Component
persistent identity: component1940135
display id: component1940135
version: 1
top level: BBa_I715037
access: public
definition: https://synbiohub.org/public/igem/BBa_I715020/1

BBa_I715019
type: Component
persistent identity: component1940159
display id: component1940159
version: 1
top level: BBa_I715037
access: public
definition: https://synbiohub.org/public/igem/BBa_I715019/1

BBa_I715023
type: Component
persistent identity: component1940154
display id: component1940154
version: 1
top level: BBa_I715037
access: public
definition: https://synbiohub.org/public/igem/BBa_I715023/1

BBa_J44000
type: Component
persistent identity: component1940141
display id: component1940141
version: 1
top level: BBa_I715037
access: public
definition: hixC

BBa_I715020
type: Component
persistent identity: component1940144
display id: component1940144
version: 1
top level: BBa_I715037
access: public
definition: https://synbiohub.org/public/igem/BBa_I715020/1

BBa_B0034
type: Component
persistent identity: component1940128
display id: component1940128
version: 1
top level: BBa_I715037
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_J44000
type: Component
persistent identity: component1940132
display id: component1940132
version: 1
top level: BBa_I715037
access: public
definition: hixC

BBa_B0010
type: Component
persistent identity: component1940145
display id: component1940145
version: 1
top level: BBa_I715037
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_R0085
type: Component
persistent identity: component1940126
display id: component1940126
version: 1
top level: BBa_I715037
access: public
definition: https://synbiohub.org/public/igem/BBa_R0085/1

BBa_B0034
type: Component
persistent identity: component1940137
display id: component1940137
version: 1
top level: BBa_I715037
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_J44000
type: Component
persistent identity: component1940160
display id: component1940160
version: 1
top level: BBa_I715037
access: public
definition: hixC

BBa_I715022
type: Component
persistent identity: component1940131
display id: component1940131
version: 1
top level: BBa_I715037
access: public
definition: https://synbiohub.org/public/igem/BBa_I715022/1

BBa_J44000
type: SequenceAnnotation
persistent identity: annotation1940160
display id: annotation1940160
version: 1
top level: BBa_I715037
location: https://synbiohub.org/public/igem/BBa_I715037/annotation1940160/range1940160/1
component: BBa_J44000

https://synbiohub.org/public/igem/BBa_I715037/annotation1940160/range1940160/1
type: Range
persistent identity: range1940160
display id: range1940160
version: 1
direction: direction/reverse
top level: BBa_I715037
start: 2488
end: 2513
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1940156
display id: annotation1940156
version: 1
top level: BBa_I715037
location: https://synbiohub.org/public/igem/BBa_I715037/annotation1940156/range1940156/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I715037/annotation1940156/range1940156/1
type: Range
persistent identity: range1940156
display id: range1940156
version: 1
direction: direction/reverse
top level: BBa_I715037
start: 1991
end: 2002
orientation: inline

BBa_I715020
type: SequenceAnnotation
persistent identity: annotation1940144
display id: annotation1940144
version: 1
top level: BBa_I715037
location: https://synbiohub.org/public/igem/BBa_I715037/annotation1940144/range1940144/1
component: BBa_I715020

https://synbiohub.org/public/igem/BBa_I715037/annotation1940144/range1940144/1
type: Range
persistent identity: range1940144
display id: range1940144
version: 1
direction: direction/reverse
top level: BBa_I715037
start: 1334
end: 1583
orientation: inline

BBa_I715023
type: SequenceAnnotation
persistent identity: annotation1940154
display id: annotation1940154
version: 1
top level: BBa_I715037
location: https://synbiohub.org/public/igem/BBa_I715037/annotation1940154/range1940154/1
component: BBa_I715023

https://synbiohub.org/public/igem/BBa_I715037/annotation1940154/range1940154/1
type: Range
persistent identity: range1940154
display id: range1940154
version: 1
direction: direction/reverse
top level: BBa_I715037
start: 1763
end: 1982
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1940147
display id: annotation1940147
version: 1
top level: BBa_I715037
location: https://synbiohub.org/public/igem/BBa_I715037/annotation1940147/range1940147/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I715037/annotation1940147/range1940147/1
type: Range
persistent identity: range1940147
display id: range1940147
version: 1
direction: direction/forward
top level: BBa_I715037
start: 1680
end: 1720
orientation: inline

BBa_J44000
type: SequenceAnnotation
persistent identity: annotation1940132
display id: annotation1940132
version: 1
top level: BBa_I715037
location: https://synbiohub.org/public/igem/BBa_I715037/annotation1940132/range1940132/1
component: BBa_J44000

https://synbiohub.org/public/igem/BBa_I715037/annotation1940132/range1940132/1
type: Range
persistent identity: range1940132
display id: range1940132
version: 1
direction: direction/reverse
top level: BBa_I715037
start: 520
end: 545
orientation: inline

BBa_I715022
type: SequenceAnnotation
persistent identity: annotation1940140
display id: annotation1940140
version: 1
top level: BBa_I715037
location: https://synbiohub.org/public/igem/BBa_I715037/annotation1940140/range1940140/1
component: BBa_I715022

https://synbiohub.org/public/igem/BBa_I715037/annotation1940140/range1940140/1
type: Range
persistent identity: range1940140
display id: range1940140
version: 1
direction: direction/reverse
top level: BBa_I715037
start: 830
end: 1291
orientation: inline

BBa_I715020
type: SequenceAnnotation
persistent identity: annotation1940135
display id: annotation1940135
version: 1
top level: BBa_I715037
location: https://synbiohub.org/public/igem/BBa_I715037/annotation1940135/range1940135/1
component: BBa_I715020

https://synbiohub.org/public/igem/BBa_I715037/annotation1940135/range1940135/1
type: Range
persistent identity: range1940135
display id: range1940135
version: 1
direction: direction/reverse
top level: BBa_I715037
start: 554
end: 803
orientation: inline

BBa_R0085
type: SequenceAnnotation
persistent identity: annotation1940126
display id: annotation1940126
version: 1
top level: BBa_I715037
location: https://synbiohub.org/public/igem/BBa_I715037/annotation1940126/range1940126/1
component: BBa_R0085

https://synbiohub.org/public/igem/BBa_I715037/annotation1940126/range1940126/1
type: Range
persistent identity: range1940126
display id: range1940126
version: 1
direction: direction/reverse
top level: BBa_I715037
start: 1
end: 23
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1940145
display id: annotation1940145
version: 1
top level: BBa_I715037
location: https://synbiohub.org/public/igem/BBa_I715037/annotation1940145/range1940145/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I715037/annotation1940145/range1940145/1
type: Range
persistent identity: range1940145
display id: range1940145
version: 1
direction: direction/forward
top level: BBa_I715037
start: 1592
end: 1671
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1940137
display id: annotation1940137
version: 1
top level: BBa_I715037
location: https://synbiohub.org/public/igem/BBa_I715037/annotation1940137/range1940137/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I715037/annotation1940137/range1940137/1
type: Range
persistent identity: range1940137
display id: range1940137
version: 1
direction: direction/reverse
top level: BBa_I715037
start: 812
end: 823
orientation: inline

BBa_J44000
type: SequenceAnnotation
persistent identity: annotation1940151
display id: annotation1940151
version: 1
top level: BBa_I715037
location: https://synbiohub.org/public/igem/BBa_I715037/annotation1940151/range1940151/1
component: BBa_J44000

https://synbiohub.org/public/igem/BBa_I715037/annotation1940151/range1940151/1
type: Range
persistent identity: range1940151
display id: range1940151
version: 1
direction: direction/reverse
top level: BBa_I715037
start: 1729
end: 1754
orientation: inline

BBa_I715022
type: SequenceAnnotation
persistent identity: annotation1940131
display id: annotation1940131
version: 1
top level: BBa_I715037
location: https://synbiohub.org/public/igem/BBa_I715037/annotation1940131/range1940131/1
component: BBa_I715022

https://synbiohub.org/public/igem/BBa_I715037/annotation1940131/range1940131/1
type: Range
persistent identity: range1940131
display id: range1940131
version: 1
direction: direction/reverse
top level: BBa_I715037
start: 50
end: 511
orientation: inline

BBa_I715019
type: SequenceAnnotation
persistent identity: annotation1940159
display id: annotation1940159
version: 1
top level: BBa_I715037
location: https://synbiohub.org/public/igem/BBa_I715037/annotation1940159/range1940159/1
component: BBa_I715019

https://synbiohub.org/public/igem/BBa_I715037/annotation1940159/range1940159/1
type: Range
persistent identity: range1940159
display id: range1940159
version: 1
direction: direction/reverse
top level: BBa_I715037
start: 2009
end: 2479
orientation: inline

BBa_J44000
type: SequenceAnnotation
persistent identity: annotation1940141
display id: annotation1940141
version: 1
top level: BBa_I715037
location: https://synbiohub.org/public/igem/BBa_I715037/annotation1940141/range1940141/1
component: BBa_J44000

https://synbiohub.org/public/igem/BBa_I715037/annotation1940141/range1940141/1
type: Range
persistent identity: range1940141
display id: range1940141
version: 1
direction: direction/reverse
top level: BBa_I715037
start: 1300
end: 1325
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1940128
display id: annotation1940128
version: 1
top level: BBa_I715037
location: https://synbiohub.org/public/igem/BBa_I715037/annotation1940128/range1940128/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I715037/annotation1940128/range1940128/1
type: Range
persistent identity: range1940128
display id: range1940128
version: 1
direction: direction/reverse
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start: 32
end: 43
orientation: inline

BBa_I715043
type: ComponentDefinition
persistent identity: BBa I715043
display id: BBa_I715043
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: HPP-A1
creator: Tom Crowley
created: 2007-08-22T11:00:00Z
modified: 2015-08-31T04:07:49Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _120_
m user id: 0
owner id: 1803
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: T7prom-RBS-RFP1-hixC-RFP2-RBS-GFP1-hixC-RFP2-TT-hixC-GFP2-TT-hixC
mutable notes: None
mutable provenance: Built from construction intermediates
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0012
component: BBa_J44000
component: BBa_I715023
component: BBa_I715019
component: BBa_B0034
component: BBa_I715020
component: BBa_R0085
component: BBa_B0010
component: BBa_I715022
sequence annotation: BBa_J44000
sequence annotation: BBa_B0010
sequence annotation: BBa_I715023
sequence annotation: BBa_I715020
sequence annotation: BBa_B0012
sequence annotation: BBa_I715022
sequence annotation: BBa_B0034
sequence annotation: BBa_R0085
sequence annotation: BBa_I715019
sequence: https://synbiohub.org/public/igem/BBa_I715043_sequence/1

BBa_B0012
type: Component
persistent identity: component1942400
display id: component1942400
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access: public
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BBa_J44000
type: Component
persistent identity: component1942384
display id: component1942384
version: 1
top level: BBa_I715043
access: public
definition: hixC

BBa_B0012
type: Component
persistent identity: component1942390
display id: component1942390
version: 1
top level: BBa_I715043
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_J44000
type: Component
persistent identity: component1942375
display id: component1942375
version: 1
top level: BBa_I715043
access: public
definition: hixC

BBa_I715023
type: Component
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display id: component1942387
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access: public
definition: https://synbiohub.org/public/igem/BBa_I715023/1

BBa_I715019
type: Component
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display id: component1942383
version: 1
top level: BBa_I715043
access: public
definition: https://synbiohub.org/public/igem/BBa_I715019/1

BBa_B0034
type: Component
persistent identity: component1942380
display id: component1942380
version: 1
top level: BBa_I715043
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_J44000
type: Component
persistent identity: component1942394
display id: component1942394
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top level: BBa_I715043
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definition: hixC

BBa_I715020
type: Component
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definition: https://synbiohub.org/public/igem/BBa_I715020/1

BBa_B0034
type: Component
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display id: component1942371
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definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_I715023
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definition: https://synbiohub.org/public/igem/BBa_I715023/1

BBa_R0085
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BBa_B0010
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definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0010
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definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_J44000
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display id: component1942404
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definition: hixC

BBa_I715022
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definition: https://synbiohub.org/public/igem/BBa_I715022/1

BBa_J44000
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BBa_B0010
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BBa_I715023
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BBa_J44000
type: SequenceAnnotation
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BBa_J44000
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BBa_I715020
type: SequenceAnnotation
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persistent identity: range1942397
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direction: direction/reverse
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BBa_B0012
type: SequenceAnnotation
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BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1942400
display id: annotation1942400
version: 1
top level: BBa_I715043
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display id: range1942400
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BBa_J44000
type: SequenceAnnotation
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BBa_I715022
type: SequenceAnnotation
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display id: annotation1942374
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BBa_B0034
type: SequenceAnnotation
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BBa_I715023
type: SequenceAnnotation
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type: Range
persistent identity: range1942378
display id: range1942378
version: 1
direction: direction/reverse
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start: 554
end: 773
orientation: inline

BBa_R0085
type: SequenceAnnotation
persistent identity: annotation1942369
display id: annotation1942369
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location: https://synbiohub.org/public/igem/BBa_I715043/annotation1942369/range1942369/1
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type: Range
persistent identity: range1942369
display id: range1942369
version: 1
direction: direction/reverse
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start: 1
end: 23
orientation: inline

BBa_I715019
type: SequenceAnnotation
persistent identity: annotation1942383
display id: annotation1942383
version: 1
top level: BBa_I715043
location: https://synbiohub.org/public/igem/BBa_I715043/annotation1942383/range1942383/1
component: BBa_I715019

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persistent identity: range1942383
display id: range1942383
version: 1
direction: direction/reverse
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end: 1270
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1942398
display id: annotation1942398
version: 1
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type: Range
persistent identity: range1942398
display id: range1942398
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end: 2049
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1942380
display id: annotation1942380
version: 1
top level: BBa_I715043
location: https://synbiohub.org/public/igem/BBa_I715043/annotation1942380/range1942380/1
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type: Range
persistent identity: range1942380
display id: range1942380
version: 1
direction: direction/reverse
top level: BBa_I715043
start: 782
end: 793
orientation: inline

BBa_J44010
type: ComponentDefinition
persistent identity: BBa J44010
display id: BBa_J44010
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: HixC-pBAD-HixC-RBS-TetF-HixC
creator: Todd Eckdahl
created: 2006-10-25T11:00:00Z
modified: 2015-08-31T04:08:49Z
discontinued: false
dominant: true
experience: experience/None
group u list: _71_
m user id: 0
owner id: 606
owning group id: 61
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Construct for use in detecting the activity of Hin recombinase system. pBAD promoter is flanked by HixC recombination sites and may be flipped from the right to the left orientation. Tet resistance gene with upsteam RBS are also flanked by HixC sites and may be flipped from forward to reverse orientation.
mutable notes: None
mutable provenance: HixC is synthetic DNA, rest of construct is part J44003.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_I13453
component: BBa_B0030
component: BBa_J44000
component: BBa_J31007
sequence annotation: BBa_I13453
sequence annotation: BBa_J44000
sequence annotation: BBa_B0030
sequence annotation: BBa_J31007
sequence: https://synbiohub.org/public/igem/BBa_J44010_sequence/1

BBa_I13453
type: Component
persistent identity: component1905171
display id: component1905171
version: 1
top level: BBa_J44010
access: public
definition: https://synbiohub.org/public/igem/BBa_I13453/1

BBa_B0030
type: Component
persistent identity: component1905174
display id: component1905174
version: 1
top level: BBa_J44010
access: public
definition: https://synbiohub.org/public/igem/BBa_B0030/1

BBa_J44000
type: Component
persistent identity: component1905178
display id: component1905178
version: 1
top level: BBa_J44010
access: public
definition: hixC

BBa_J44000
type: Component
persistent identity: component1905172
display id: component1905172
version: 1
top level: BBa_J44010
access: public
definition: hixC

BBa_J44000
type: Component
persistent identity: component1905170
display id: component1905170
version: 1
top level: BBa_J44010
access: public
definition: hixC

BBa_J31007
type: Component
persistent identity: component1905177
display id: component1905177
version: 1
top level: BBa_J44010
access: public
definition: https://synbiohub.org/public/igem/BBa_J31007/1

BBa_I13453
type: SequenceAnnotation
persistent identity: annotation1905171
display id: annotation1905171
version: 1
top level: BBa_J44010
location: https://synbiohub.org/public/igem/BBa_J44010/annotation1905171/range1905171/1
component: BBa_I13453

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type: Range
persistent identity: range1905171
display id: range1905171
version: 1
direction: direction/reverse
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start: 35
end: 164
orientation: inline

BBa_J44000
type: SequenceAnnotation
persistent identity: annotation1905170
display id: annotation1905170
version: 1
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location: https://synbiohub.org/public/igem/BBa_J44010/annotation1905170/range1905170/1
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persistent identity: range1905170
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direction: direction/reverse
top level: BBa_J44010
start: 1
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orientation: inline

BBa_B0030
type: SequenceAnnotation
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display id: annotation1905174
version: 1
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display id: range1905174
version: 1
direction: direction/forward
top level: BBa_J44010
start: 207
end: 221
orientation: inline

BBa_J31007
type: SequenceAnnotation
persistent identity: annotation1905177
display id: annotation1905177
version: 1
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location: https://synbiohub.org/public/igem/BBa_J44010/annotation1905177/range1905177/1
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persistent identity: range1905177
display id: range1905177
version: 1
direction: direction/reverse
top level: BBa_J44010
start: 228
end: 1418
orientation: inline

BBa_J44000
type: SequenceAnnotation
persistent identity: annotation1905172
display id: annotation1905172
version: 1
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location: https://synbiohub.org/public/igem/BBa_J44010/annotation1905172/range1905172/1
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persistent identity: range1905172
display id: range1905172
version: 1
direction: direction/reverse
top level: BBa_J44010
start: 173
end: 198
orientation: inline

BBa_J44000
type: SequenceAnnotation
persistent identity: annotation1905178
display id: annotation1905178
version: 1
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persistent identity: range1905178
display id: range1905178
version: 1
direction: direction/reverse
top level: BBa_J44010
start: 1427
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orientation: inline

BBa_I715042
type: ComponentDefinition
persistent identity: BBa I715042
display id: BBa_I715042
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: HPP-A0
creator: Tom Crowley
created: 2007-08-22T11:00:00Z
modified: 2015-08-31T04:07:49Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _120_
m user id: 0
owner id: 1803
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: T7prom-RBS-RFP1-hixC-RFP2-RBS-GFP1-hixC-GFP2-TT-hixC-RFP2-TT-hixC
mutable notes: None
mutable provenance: Built from construction intermediates.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0010
component: BBa_B0012
component: BBa_J44000
component: BBa_I715019
component: BBa_I715023
component: BBa_B0034
component: BBa_I715020
component: BBa_R0085
component: BBa_I715022
sequence annotation: BBa_J44000
sequence annotation: BBa_I715023
sequence annotation: BBa_B0010
sequence annotation: BBa_B0034
sequence annotation: BBa_B0012
sequence annotation: BBa_I715020
sequence annotation: BBa_R0085
sequence annotation: BBa_I715019
sequence annotation: BBa_I715022
sequence: https://synbiohub.org/public/igem/BBa_I715042_sequence/1

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persistent identity: component1942349
display id: component1942349
version: 1
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access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0012
type: Component
persistent identity: component1942361
display id: component1942361
version: 1
top level: BBa_I715042
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_J44000
type: Component
persistent identity: component1942355
display id: component1942355
version: 1
top level: BBa_I715042
access: public
definition: hixC

BBa_I715019
type: Component
persistent identity: component1942344
display id: component1942344
version: 1
top level: BBa_I715042
access: public
definition: https://synbiohub.org/public/igem/BBa_I715019/1

BBa_I715023
type: Component
persistent identity: component1942339
display id: component1942339
version: 1
top level: BBa_I715042
access: public
definition: https://synbiohub.org/public/igem/BBa_I715023/1

BBa_B0034
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persistent identity: component1942332
display id: component1942332
version: 1
top level: BBa_I715042
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_I715020
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persistent identity: component1942348
display id: component1942348
version: 1
top level: BBa_I715042
access: public
definition: https://synbiohub.org/public/igem/BBa_I715020/1

BBa_B0010
type: Component
persistent identity: component1942359
display id: component1942359
version: 1
top level: BBa_I715042
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_J44000
type: Component
persistent identity: component1942336
display id: component1942336
version: 1
top level: BBa_I715042
access: public
definition: hixC

BBa_R0085
type: Component
persistent identity: component1942330
display id: component1942330
version: 1
top level: BBa_I715042
access: public
definition: https://synbiohub.org/public/igem/BBa_R0085/1

BBa_J44000
type: Component
persistent identity: component1942365
display id: component1942365
version: 1
top level: BBa_I715042
access: public
definition: hixC

BBa_I715022
type: Component
persistent identity: component1942335
display id: component1942335
version: 1
top level: BBa_I715042
access: public
definition: https://synbiohub.org/public/igem/BBa_I715022/1

BBa_J44000
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display id: component1942345
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definition: hixC

BBa_B0034
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definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_I715023
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BBa_B0012
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BBa_B0010
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BBa_J44000
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BBa_B0034
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BBa_J44000
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BBa_B0034
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persistent identity: annotation1942341
display id: annotation1942341
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top level: BBa_I715042
location: https://synbiohub.org/public/igem/BBa_I715042/annotation1942341/range1942341/1
component: BBa_B0034

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persistent identity: range1942341
display id: range1942341
version: 1
direction: direction/reverse
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start: 782
end: 793
orientation: inline

BBa_I715023
type: SequenceAnnotation
persistent identity: annotation1942358
display id: annotation1942358
version: 1
top level: BBa_I715042
location: https://synbiohub.org/public/igem/BBa_I715042/annotation1942358/range1942358/1
component: BBa_I715023

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type: Range
persistent identity: range1942358
display id: range1942358
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direction: direction/reverse
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BBa_B0010
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persistent identity: annotation1942349
display id: annotation1942349
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display id: range1942349
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BBa_B0012
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display id: annotation1942361
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top level: BBa_I715042
location: https://synbiohub.org/public/igem/BBa_I715042/annotation1942361/range1942361/1
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display id: range1942361
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BBa_J44000
type: SequenceAnnotation
persistent identity: annotation1942345
display id: annotation1942345
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location: https://synbiohub.org/public/igem/BBa_I715042/annotation1942345/range1942345/1
component: BBa_J44000

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persistent identity: range1942345
display id: range1942345
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BBa_I715020
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display id: annotation1942348
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top level: BBa_I715042
location: https://synbiohub.org/public/igem/BBa_I715042/annotation1942348/range1942348/1
component: BBa_I715020

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type: Range
persistent identity: range1942348
display id: range1942348
version: 1
direction: direction/reverse
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start: 1313
end: 1562
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1942351
display id: annotation1942351
version: 1
top level: BBa_I715042
location: https://synbiohub.org/public/igem/BBa_I715042/annotation1942351/range1942351/1
component: BBa_B0012

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type: Range
persistent identity: range1942351
display id: range1942351
version: 1
direction: direction/forward
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start: 1659
end: 1699
orientation: inline

BBa_R0085
type: SequenceAnnotation
persistent identity: annotation1942330
display id: annotation1942330
version: 1
top level: BBa_I715042
location: https://synbiohub.org/public/igem/BBa_I715042/annotation1942330/range1942330/1
component: BBa_R0085

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type: Range
persistent identity: range1942330
display id: range1942330
version: 1
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start: 1
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BBa_I715019
type: SequenceAnnotation
persistent identity: annotation1942344
display id: annotation1942344
version: 1
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location: https://synbiohub.org/public/igem/BBa_I715042/annotation1942344/range1942344/1
component: BBa_I715019

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type: Range
persistent identity: range1942344
display id: range1942344
version: 1
direction: direction/reverse
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end: 1270
orientation: inline

BBa_I715022
type: SequenceAnnotation
persistent identity: annotation1942335
display id: annotation1942335
version: 1
top level: BBa_I715042
location: https://synbiohub.org/public/igem/BBa_I715042/annotation1942335/range1942335/1
component: BBa_I715022

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type: Range
persistent identity: range1942335
display id: range1942335
version: 1
direction: direction/reverse
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start: 50
end: 511
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BBa_J44012
type: ComponentDefinition
persistent identity: BBa J44012
display id: BBa_J44012
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: HixC-pBAD-HixC-RBS-TetF-HixC
creator: Todd Eckdahl
created: 2006-10-25T11:00:00Z
modified: 2015-08-31T04:08:49Z
discontinued: false
dominant: true
group u list: _71_
m user id: 0
owner id: 606
owning group id: 61
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Construct for use in detecting the activity of Hin recombinase system. pBAD promoter is flanked by HixC recombination sites and may be flipped from the right to the left orientation. Tet resistance gene with upsteam RBS are also flanked by HixC sites and may be flipped from forward to reverse orientation. Cloned in pSB1A7 - readthrough transcription not detected.
mutable notes: Trascription readthrough into part appears to have been blocked by the introduction of double forward terminators before and after the cloning site.
mutable provenance: Cloned from BBa_J44010
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_J31007
component: BBa_I13453
component: BBa_J44000
component: BBa_B0030
sequence annotation: BBa_J44000
sequence annotation: BBa_I13453
sequence annotation: BBa_J31007
sequence annotation: BBa_B0030
sequence: https://synbiohub.org/public/igem/BBa_J44012_sequence/1

BBa_J31007
type: Component
persistent identity: component1905203
display id: component1905203
version: 1
top level: BBa_J44012
access: public
definition: https://synbiohub.org/public/igem/BBa_J31007/1

BBa_I13453
type: Component
persistent identity: component1905197
display id: component1905197
version: 1
top level: BBa_J44012
access: public
definition: https://synbiohub.org/public/igem/BBa_I13453/1

BBa_J44000
type: Component
persistent identity: component1905196
display id: component1905196
version: 1
top level: BBa_J44012
access: public
definition: hixC

BBa_J44000
type: Component
persistent identity: component1905204
display id: component1905204
version: 1
top level: BBa_J44012
access: public
definition: hixC

BBa_B0030
type: Component
persistent identity: component1905200
display id: component1905200
version: 1
top level: BBa_J44012
access: public
definition: https://synbiohub.org/public/igem/BBa_B0030/1

BBa_J44000
type: Component
persistent identity: component1905198
display id: component1905198
version: 1
top level: BBa_J44012
access: public
definition: hixC

BBa_J44000
type: SequenceAnnotation
persistent identity: annotation1905196
display id: annotation1905196
version: 1
top level: BBa_J44012
location: https://synbiohub.org/public/igem/BBa_J44012/annotation1905196/range1905196/1
component: BBa_J44000

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persistent identity: range1905196
display id: range1905196
version: 1
direction: direction/reverse
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start: 1
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orientation: inline

BBa_I13453
type: SequenceAnnotation
persistent identity: annotation1905197
display id: annotation1905197
version: 1
top level: BBa_J44012
location: https://synbiohub.org/public/igem/BBa_J44012/annotation1905197/range1905197/1
component: BBa_I13453

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persistent identity: range1905197
display id: range1905197
version: 1
direction: direction/reverse
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start: 35
end: 164
orientation: inline

BBa_J31007
type: SequenceAnnotation
persistent identity: annotation1905203
display id: annotation1905203
version: 1
top level: BBa_J44012
location: https://synbiohub.org/public/igem/BBa_J44012/annotation1905203/range1905203/1
component: BBa_J31007

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persistent identity: range1905203
display id: range1905203
version: 1
direction: direction/reverse
top level: BBa_J44012
start: 228
end: 1418
orientation: inline

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type: SequenceAnnotation
persistent identity: annotation1905204
display id: annotation1905204
version: 1
top level: BBa_J44012
location: https://synbiohub.org/public/igem/BBa_J44012/annotation1905204/range1905204/1
component: BBa_J44000

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type: Range
persistent identity: range1905204
display id: range1905204
version: 1
direction: direction/reverse
top level: BBa_J44012
start: 1427
end: 1452
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation1905200
display id: annotation1905200
version: 1
top level: BBa_J44012
location: https://synbiohub.org/public/igem/BBa_J44012/annotation1905200/range1905200/1
component: BBa_B0030

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persistent identity: range1905200
display id: range1905200
version: 1
direction: direction/forward
top level: BBa_J44012
start: 207
end: 221
orientation: inline

BBa_J44000
type: SequenceAnnotation
persistent identity: annotation1905198
display id: annotation1905198
version: 1
top level: BBa_J44012
location: https://synbiohub.org/public/igem/BBa_J44012/annotation1905198/range1905198/1
component: BBa_J44000

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type: Range
persistent identity: range1905198
display id: range1905198
version: 1
direction: direction/reverse
top level: BBa_J44012
start: 173
end: 198
orientation: inline

BBa_I715047
type: ComponentDefinition
persistent identity: BBa I715047
display id: BBa_I715047
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: HPP-A0 + Hin
creator: Amanda Valencia
created: 2007-10-23T11:00:00Z
modified: 2015-08-31T04:07:50Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _120_
m user id: 0
owner id: 1858
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: Part is composed of a Hin recombinase expression casette driven by pLac and series of flippable units that include halves of GFP and RFP: pLac-RBS-Hin-LVA-T7prom-RBS-RFP1-hixC-RFP2-RBS-GFP1-hixC-GFP2-TT-hixC-RFP2-TT-hixC. The corresponding part without Hin glows yellow as a result of the expression of the hix-interrupted GFP and RFP. With Hin, most colonies are yellow, but red and green colonies appear. Evidence suggests that this is a result of Hin-mediated recombination.
mutable notes: None
mutable provenance: Ligation of exisiting interemediates
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0012
component: BBa_J44000
component: BBa_B0010
component: BBa_B0030
component: BBa_I715019
component: BBa_B0034
component: BBa_I715023
component: BBa_R0010
component: BBa_I715022
component: BBa_R0085
component: BBa_J31001
component: BBa_I715020
sequence annotation: BBa_J31001
sequence annotation: BBa_J44000
sequence annotation: BBa_I715020
sequence annotation: BBa_R0010
sequence annotation: BBa_B0012
sequence annotation: BBa_R0085
sequence annotation: BBa_I715023
sequence annotation: BBa_B0030
sequence annotation: BBa_B0034
sequence annotation: BBa_I715019
sequence annotation: BBa_B0010
sequence annotation: BBa_I715022
sequence: https://synbiohub.org/public/igem/BBa_I715047_sequence/1

BBa_B0012
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persistent identity: component1954093
display id: component1954093
version: 1
top level: BBa_I715047
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_J44000
type: Component
persistent identity: component1954087
display id: component1954087
version: 1
top level: BBa_I715047
access: public
definition: hixC

BBa_B0010
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persistent identity: component1954091
display id: component1954091
version: 1
top level: BBa_I715047
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0010
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persistent identity: component1954053
display id: component1954053
version: 1
top level: BBa_I715047
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0012
type: Component
persistent identity: component1954055
display id: component1954055
version: 1
top level: BBa_I715047
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0030
type: Component
persistent identity: component1954045
display id: component1954045
version: 1
top level: BBa_I715047
access: public
definition: https://synbiohub.org/public/igem/BBa_B0030/1

BBa_I715019
type: Component
persistent identity: component1954076
display id: component1954076
version: 1
top level: BBa_I715047
access: public
definition: https://synbiohub.org/public/igem/BBa_I715019/1

BBa_B0034
type: Component
persistent identity: component1954073
display id: component1954073
version: 1
top level: BBa_I715047
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_J44000
type: Component
persistent identity: component1954097
display id: component1954097
version: 1
top level: BBa_I715047
access: public
definition: hixC

BBa_I715023
type: Component
persistent identity: component1954090
display id: component1954090
version: 1
top level: BBa_I715047
access: public
definition: https://synbiohub.org/public/igem/BBa_I715023/1

BBa_B0034
type: Component
persistent identity: component1954064
display id: component1954064
version: 1
top level: BBa_I715047
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_R0010
type: Component
persistent identity: component1954037
display id: component1954037
version: 1
top level: BBa_I715047
access: public
definition: https://synbiohub.org/public/igem/BBa_R0010/1

BBa_I715022
type: Component
persistent identity: component1954067
display id: component1954067
version: 1
top level: BBa_I715047
access: public
definition: https://synbiohub.org/public/igem/BBa_I715022/1

BBa_J44000
type: Component
persistent identity: component1954068
display id: component1954068
version: 1
top level: BBa_I715047
access: public
definition: hixC

BBa_J44000
type: Component
persistent identity: component1954077
display id: component1954077
version: 1
top level: BBa_I715047
access: public
definition: hixC

BBa_R0085
type: Component
persistent identity: component1954062
display id: component1954062
version: 1
top level: BBa_I715047
access: public
definition: https://synbiohub.org/public/igem/BBa_R0085/1

BBa_B0010
type: Component
persistent identity: component1954081
display id: component1954081
version: 1
top level: BBa_I715047
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_J31001
type: Component
persistent identity: component1954052
display id: component1954052
version: 1
top level: BBa_I715047
access: public
definition: Hin LVA

BBa_I715023
type: Component
persistent identity: component1954071
display id: component1954071
version: 1
top level: BBa_I715047
access: public
definition: https://synbiohub.org/public/igem/BBa_I715023/1

BBa_B0012
type: Component
persistent identity: component1954083
display id: component1954083
version: 1
top level: BBa_I715047
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_I715020
type: Component
persistent identity: component1954080
display id: component1954080
version: 1
top level: BBa_I715047
access: public
definition: https://synbiohub.org/public/igem/BBa_I715020/1

BBa_J31001
type: SequenceAnnotation
persistent identity: annotation1954052
display id: annotation1954052
version: 1
top level: BBa_I715047
location: https://synbiohub.org/public/igem/BBa_I715047/annotation1954052/range1954052/1
component: BBa_J31001

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type: Range
persistent identity: range1954052
display id: range1954052
version: 1
direction: direction/reverse
top level: BBa_I715047
start: 230
end: 841
orientation: inline

BBa_J44000
type: SequenceAnnotation
persistent identity: annotation1954068
display id: annotation1954068
version: 1
top level: BBa_I715047
location: https://synbiohub.org/public/igem/BBa_I715047/annotation1954068/range1954068/1
component: BBa_J44000

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type: Range
persistent identity: range1954068
display id: range1954068
version: 1
direction: direction/reverse
top level: BBa_I715047
start: 1506
end: 1531
orientation: inline

BBa_I715020
type: SequenceAnnotation
persistent identity: annotation1954080
display id: annotation1954080
version: 1
top level: BBa_I715047
location: https://synbiohub.org/public/igem/BBa_I715047/annotation1954080/range1954080/1
component: BBa_I715020

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type: Range
persistent identity: range1954080
display id: range1954080
version: 1
direction: direction/reverse
top level: BBa_I715047
start: 2299
end: 2548
orientation: inline

BBa_R0010
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BBa_I715051
type: ComponentDefinition
persistent identity: BBa I715051
display id: BBa_I715051
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: HPP-A2 + Hin
creator: Amanda Valencia
created: 2007-10-23T11:00:00Z
modified: 2015-08-31T04:07:50Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _120_
m user id: 0
owner id: 1858
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: Part is composed of a Hin recombinase expression casette driven by pLac and series of flippable units that include halves of GFP and RFP: T7prom-RBS-RFP1-hixC-GFP2-TT-hixC-RFP2-RBS-GFP1-hixC-RFP2-TT-hixC. The corresponding part without Hin does not glow. With Hin, most colonies do not glow, but green and red colonies appear. Evidence suggests that this is a result of Hin-mediated recombination.
mutable notes: None
mutable provenance: Ligation of existing parts
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_I715022
component: BBa_B0010
component: BBa_J31001
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component: BBa_I715019
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sequence annotation: BBa_B0030
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BBa_I715022
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BBa_J31001
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definition: Hin LVA

BBa_I715020
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BBa_J44000
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display id: component1954340
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definition: hixC

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display id: annotation1954334
version: 1
top level: BBa_I715051
location: https://synbiohub.org/public/igem/BBa_I715051/annotation1954334/range1954334/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I715051/annotation1954334/range1954334/1
type: Range
persistent identity: range1954334
display id: range1954334
version: 1
direction: direction/forward
top level: BBa_I715051
start: 1798
end: 1877
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1954353
display id: annotation1954353
version: 1
top level: BBa_I715051
location: https://synbiohub.org/public/igem/BBa_I715051/annotation1954353/range1954353/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I715051/annotation1954353/range1954353/1
type: Range
persistent identity: range1954353
display id: range1954353
version: 1
direction: direction/forward
top level: BBa_I715051
start: 2956
end: 3035
orientation: inline

BBa_J44000
type: SequenceAnnotation
persistent identity: annotation1954359
display id: annotation1954359
version: 1
top level: BBa_I715051
location: https://synbiohub.org/public/igem/BBa_I715051/annotation1954359/range1954359/1
component: BBa_J44000

https://synbiohub.org/public/igem/BBa_I715051/annotation1954359/range1954359/1
type: Range
persistent identity: range1954359
display id: range1954359
version: 1
direction: direction/reverse
top level: BBa_I715051
start: 3093
end: 3118
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1954315
display id: annotation1954315
version: 1
top level: BBa_I715051
location: https://synbiohub.org/public/igem/BBa_I715051/annotation1954315/range1954315/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I715051/annotation1954315/range1954315/1
type: Range
persistent identity: range1954315
display id: range1954315
version: 1
direction: direction/forward
top level: BBa_I715051
start: 850
end: 929
orientation: inline

BBa_R0085
type: SequenceAnnotation
persistent identity: annotation1954324
display id: annotation1954324
version: 1
top level: BBa_I715051
location: https://synbiohub.org/public/igem/BBa_I715051/annotation1954324/range1954324/1
component: BBa_R0085

https://synbiohub.org/public/igem/BBa_I715051/annotation1954324/range1954324/1
type: Range
persistent identity: range1954324
display id: range1954324
version: 1
direction: direction/reverse
top level: BBa_I715051
start: 987
end: 1009
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1954326
display id: annotation1954326
version: 1
top level: BBa_I715051
location: https://synbiohub.org/public/igem/BBa_I715051/annotation1954326/range1954326/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I715051/annotation1954326/range1954326/1
type: Range
persistent identity: range1954326
display id: range1954326
version: 1
direction: direction/reverse
top level: BBa_I715051
start: 1018
end: 1029
orientation: inline

BBa_J44000
type: SequenceAnnotation
persistent identity: annotation1954340
display id: annotation1954340
version: 1
top level: BBa_I715051
location: https://synbiohub.org/public/igem/BBa_I715051/annotation1954340/range1954340/1
component: BBa_J44000

https://synbiohub.org/public/igem/BBa_I715051/annotation1954340/range1954340/1
type: Range
persistent identity: range1954340
display id: range1954340
version: 1
direction: direction/reverse
top level: BBa_I715051
start: 1935
end: 1960
orientation: inline

BBa_J44003
type: ComponentDefinition
persistent identity: BBa J44003
display id: BBa_J44003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: HixC-pBad-HixC-RBS-TetF
creator: Todd Eckdahl
created: 2006-08-15T11:00:00Z
modified: 2015-08-31T04:08:49Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _61_71_
m user id: 0
owner id: 606
owning group id: 61
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This is an expression cassette for Tet resistence. The pBAD promoter is between two HixC sites, allowing for Hin recombinase to flip the pBAD promoter. This can turn on and off Tet resistence.
mutable notes: The pBAD is able to be flipped by the Hin recombinase.
mutable provenance: pBAD (I13453), HixC (J44000), RBS (B0030), TetF (J31007)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_J44000
component: BBa_B0030
component: BBa_J31007
component: BBa_I13453
sequence annotation: BBa_J44000
sequence annotation: BBa_J31007
sequence annotation: BBa_I13453
sequence annotation: BBa_B0030
sequence: https://synbiohub.org/public/igem/BBa_J44003_sequence/1

BBa_J44000
type: Component
persistent identity: component1897020
display id: component1897020
version: 1
top level: BBa_J44003
access: public
definition: hixC

BBa_B0030
type: Component
persistent identity: component1897022
display id: component1897022
version: 1
top level: BBa_J44003
access: public
definition: https://synbiohub.org/public/igem/BBa_B0030/1

BBa_J44000
type: Component
persistent identity: component1897018
display id: component1897018
version: 1
top level: BBa_J44003
access: public
definition: hixC

BBa_J31007
type: Component
persistent identity: component1897025
display id: component1897025
version: 1
top level: BBa_J44003
access: public
definition: https://synbiohub.org/public/igem/BBa_J31007/1

BBa_I13453
type: Component
persistent identity: component1897019
display id: component1897019
version: 1
top level: BBa_J44003
access: public
definition: https://synbiohub.org/public/igem/BBa_I13453/1

BBa_J44000
type: SequenceAnnotation
persistent identity: annotation1897020
display id: annotation1897020
version: 1
top level: BBa_J44003
location: https://synbiohub.org/public/igem/BBa_J44003/annotation1897020/range1897020/1
component: BBa_J44000

https://synbiohub.org/public/igem/BBa_J44003/annotation1897020/range1897020/1
type: Range
persistent identity: range1897020
display id: range1897020
version: 1
direction: direction/reverse
top level: BBa_J44003
start: 173
end: 198
orientation: inline

BBa_J44000
type: SequenceAnnotation
persistent identity: annotation1897018
display id: annotation1897018
version: 1
top level: BBa_J44003
location: https://synbiohub.org/public/igem/BBa_J44003/annotation1897018/range1897018/1
component: BBa_J44000

https://synbiohub.org/public/igem/BBa_J44003/annotation1897018/range1897018/1
type: Range
persistent identity: range1897018
display id: range1897018
version: 1
direction: direction/reverse
top level: BBa_J44003
start: 1
end: 26
orientation: inline

BBa_J31007
type: SequenceAnnotation
persistent identity: annotation1897025
display id: annotation1897025
version: 1
top level: BBa_J44003
location: https://synbiohub.org/public/igem/BBa_J44003/annotation1897025/range1897025/1
component: BBa_J31007

https://synbiohub.org/public/igem/BBa_J44003/annotation1897025/range1897025/1
type: Range
persistent identity: range1897025
display id: range1897025
version: 1
direction: direction/reverse
top level: BBa_J44003
start: 228
end: 1418
orientation: inline

BBa_I13453
type: SequenceAnnotation
persistent identity: annotation1897019
display id: annotation1897019
version: 1
top level: BBa_J44003
location: https://synbiohub.org/public/igem/BBa_J44003/annotation1897019/range1897019/1
component: BBa_I13453

https://synbiohub.org/public/igem/BBa_J44003/annotation1897019/range1897019/1
type: Range
persistent identity: range1897019
display id: range1897019
version: 1
direction: direction/reverse
top level: BBa_J44003
start: 35
end: 164
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation1897022
display id: annotation1897022
version: 1
top level: BBa_J44003
location: https://synbiohub.org/public/igem/BBa_J44003/annotation1897022/range1897022/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_J44003/annotation1897022/range1897022/1
type: Range
persistent identity: range1897022
display id: range1897022
version: 1
direction: direction/forward
top level: BBa_J44003
start: 207
end: 221
orientation: inline

BBa_S03639
type: ComponentDefinition
persistent identity: BBa S03639
display id: BBa_S03639
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: (-1,-2) HixC-pBadrev-HixC : TetB-RBSrev-HixC
creator: Karmella Haynes
created: 2006-10-30T12:00:00Z
modified: 2015-05-08T01:14:26Z
discontinued: false
dominant: false
group u list: _61_
m user id: 0
owner id: 1144
owning group id: 61
sample status: It's complicated
status: status/Planning
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_S03637
component: BBa_S03636
sequence annotation: BBa_S03637
sequence annotation: BBa_S03636
sequence: https://synbiohub.org/public/igem/BBa_S03639_sequence/1

BBa_S03637
type: Component
persistent identity: component2223914
display id: component2223914
version: 1
top level: BBa_S03639
access: public
definition: https://synbiohub.org/public/igem/BBa_S03637/1

BBa_S03636
type: Component
persistent identity: component2223924
display id: component2223924
version: 1
top level: BBa_S03639
access: public
definition: https://synbiohub.org/public/igem/BBa_S03636/1

BBa_S03637
type: SequenceAnnotation
persistent identity: annotation2223914
display id: annotation2223914
version: 1
top level: BBa_S03639
location: https://synbiohub.org/public/igem/BBa_S03639/annotation2223914/range2223914/1
component: BBa_S03637

https://synbiohub.org/public/igem/BBa_S03639/annotation2223914/range2223914/1
type: Range
persistent identity: range2223914
display id: range2223914
version: 1
direction: direction/reverse
top level: BBa_S03639
start: 1
end: 198
orientation: inline

BBa_S03636
type: SequenceAnnotation
persistent identity: annotation2223924
display id: annotation2223924
version: 1
top level: BBa_S03639
location: https://synbiohub.org/public/igem/BBa_S03639/annotation2223924/range2223924/1
component: BBa_S03636

https://synbiohub.org/public/igem/BBa_S03639/annotation2223924/range2223924/1
type: Range
persistent identity: range2223924
display id: range2223924
version: 1
direction: direction/reverse
top level: BBa_S03639
start: 207
end: 1454
orientation: inline

BBa_J44009
type: ComponentDefinition
persistent identity: BBa J44009
display id: BBa_J44009
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pBAD-hixC-RBS-TetF-TT-RE
creator: Brad Ogden
created: 2006-08-23T11:00:00Z
modified: 2015-08-31T04:08:49Z
discontinued: false
dominant: false
group u list: _71_
m user id: 0
owner id: 811
owning group id: 71
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This construct was designed as a control for testing the effect of the Hin recombinase binding site hixC on transcription from the pBAD promoter.
mutable notes: None.
mutable provenance: Constructed from existing registry parts and parts created by the Davidson team.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_J44000
component: BBa_J3101
component: BBa_I13453
component: BBa_B0030
component: BBa_B0012
component: BBa_B0010
component: BBa_J31007
sequence annotation: BBa_B0030
sequence annotation: BBa_B0012
sequence annotation: BBa_J3101
sequence annotation: BBa_J44000
sequence annotation: BBa_I13453
sequence annotation: BBa_B0010
sequence annotation: BBa_J31007
sequence: https://synbiohub.org/public/igem/BBa_J44009_sequence/1

BBa_J44000
type: Component
persistent identity: component1898095
display id: component1898095
version: 1
top level: BBa_J44009
access: public
definition: hixC

BBa_J3101
type: Component
persistent identity: component1898113
display id: component1898113
version: 1
top level: BBa_J44009
access: public
definition: RE

BBa_I13453
type: Component
persistent identity: component1898094
display id: component1898094
version: 1
top level: BBa_J44009
access: public
definition: https://synbiohub.org/public/igem/BBa_I13453/1

BBa_B0030
type: Component
persistent identity: component1898097
display id: component1898097
version: 1
top level: BBa_J44009
access: public
definition: https://synbiohub.org/public/igem/BBa_B0030/1

BBa_B0012
type: Component
persistent identity: component1898103
display id: component1898103
version: 1
top level: BBa_J44009
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0010
type: Component
persistent identity: component1898101
display id: component1898101
version: 1
top level: BBa_J44009
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_J31007
type: Component
persistent identity: component1898100
display id: component1898100
version: 1
top level: BBa_J44009
access: public
definition: https://synbiohub.org/public/igem/BBa_J31007/1

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation1898097
display id: annotation1898097
version: 1
top level: BBa_J44009
location: https://synbiohub.org/public/igem/BBa_J44009/annotation1898097/range1898097/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_J44009/annotation1898097/range1898097/1
type: Range
persistent identity: range1898097
display id: range1898097
version: 1
direction: direction/forward
top level: BBa_J44009
start: 173
end: 187
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1898103
display id: annotation1898103
version: 1
top level: BBa_J44009
location: https://synbiohub.org/public/igem/BBa_J44009/annotation1898103/range1898103/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_J44009/annotation1898103/range1898103/1
type: Range
persistent identity: range1898103
display id: range1898103
version: 1
direction: direction/forward
top level: BBa_J44009
start: 1481
end: 1521
orientation: inline

BBa_J3101
type: SequenceAnnotation
persistent identity: annotation1898113
display id: annotation1898113
version: 1
top level: BBa_J44009
location: https://synbiohub.org/public/igem/BBa_J44009/annotation1898113/range1898113/1
component: BBa_J3101

https://synbiohub.org/public/igem/BBa_J44009/annotation1898113/range1898113/1
type: Range
persistent identity: range1898113
display id: range1898113
version: 1
direction: direction/reverse
top level: BBa_J44009
start: 1530
end: 1606
orientation: inline

BBa_J44000
type: SequenceAnnotation
persistent identity: annotation1898095
display id: annotation1898095
version: 1
top level: BBa_J44009
location: https://synbiohub.org/public/igem/BBa_J44009/annotation1898095/range1898095/1
component: BBa_J44000

https://synbiohub.org/public/igem/BBa_J44009/annotation1898095/range1898095/1
type: Range
persistent identity: range1898095
display id: range1898095
version: 1
direction: direction/reverse
top level: BBa_J44009
start: 139
end: 164
orientation: inline

BBa_I13453
type: SequenceAnnotation
persistent identity: annotation1898094
display id: annotation1898094
version: 1
top level: BBa_J44009
location: https://synbiohub.org/public/igem/BBa_J44009/annotation1898094/range1898094/1
component: BBa_I13453

https://synbiohub.org/public/igem/BBa_J44009/annotation1898094/range1898094/1
type: Range
persistent identity: range1898094
display id: range1898094
version: 1
direction: direction/reverse
top level: BBa_J44009
start: 1
end: 130
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1898101
display id: annotation1898101
version: 1
top level: BBa_J44009
location: https://synbiohub.org/public/igem/BBa_J44009/annotation1898101/range1898101/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_J44009/annotation1898101/range1898101/1
type: Range
persistent identity: range1898101
display id: range1898101
version: 1
direction: direction/forward
top level: BBa_J44009
start: 1393
end: 1472
orientation: inline

BBa_J31007
type: SequenceAnnotation
persistent identity: annotation1898100
display id: annotation1898100
version: 1
top level: BBa_J44009
location: https://synbiohub.org/public/igem/BBa_J44009/annotation1898100/range1898100/1
component: BBa_J31007

https://synbiohub.org/public/igem/BBa_J44009/annotation1898100/range1898100/1
type: Range
persistent identity: range1898100
display id: range1898100
version: 1
direction: direction/reverse
top level: BBa_J44009
start: 194
end: 1384
orientation: inline

BBa_J44013
type: ComponentDefinition
persistent identity: BBa J44013
display id: BBa_J44013
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: HixC-pBAD-HixC-Tetrev-RBSrev-HixC
creator: Todd Eckdahl
created: 2006-10-25T11:00:00Z
modified: 2015-08-31T04:08:49Z
discontinued: false
dominant: true
experience: experience/None
group u list: _71_
m user id: 0
owner id: 606
owning group id: 61
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Construct for use in detecting the activity of Hin recombinase system. pBAD promoter is flanked by HixC recombination sites and may be flipped from the right to the left orientation. Tet resistance gene with upsteam RBS are also flanked by HixC sites and may be flipped from reverse to forward orientation. Cloned in pSB1A7 - no readthrough transcription detected.
mutable notes: Trascription readthrough into part appears to have been blocked by the introduction of double forward terminators before and after the cloning site.
mutable provenance: Cloned from J44011.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_J44011
sequence annotation: BBa_J44011
sequence: https://synbiohub.org/public/igem/BBa_J44013_sequence/1

BBa_J44011
type: Component
persistent identity: component2223909
display id: component2223909
version: 1
top level: BBa_J44013
access: public
definition: BBa_J44011

BBa_J44011
type: SequenceAnnotation
persistent identity: annotation2223909
display id: annotation2223909
version: 1
top level: BBa_J44013
location: https://synbiohub.org/public/igem/BBa_J44013/annotation2223909/range2223909/1
component: BBa_J44011

https://synbiohub.org/public/igem/BBa_J44013/annotation2223909/range2223909/1
type: Range
persistent identity: range2223909
display id: range2223909
version: 1
direction: direction/reverse
top level: BBa_J44013
start: 1
end: 1454
orientation: inline

BBa_J44011
type: ComponentDefinition
persistent identity: BBa J44011
display id: BBa_J44011
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: HixC-pBAD-HixC-Tetrev-RBSrev-HixC
creator: Todd Eckdahl
created: 2006-10-25T11:00:00Z
modified: 2015-08-31T04:08:49Z
discontinued: false
dominant: true
experience: experience/None
group u list: _71_
m user id: 0
owner id: 606
owning group id: 61
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Construct for use in detecting the activity of Hin recombinase system. pBAD promoter is flanked by HixC recombination sites and may be flipped from the right to the left orientation. Tet resistance gene with upsteam RBS are also flanked by HixC sites and may be flipped from reverse to forward orientation.
mutable notes: None.
mutable provenance: HixC is synthetic, rest is from part BBa_S03626.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_J44000
component: BBa_S03624
sequence annotation: BBa_J44000
sequence annotation: BBa_S03624
sequence: https://synbiohub.org/public/igem/BBa_J44011_sequence/1

BBa_J44000
type: Component
persistent identity: component2223878
display id: component2223878
version: 1
top level: BBa_J44011
access: public
definition: hixC

BBa_S03624
type: Component
persistent identity: component2223892
display id: component2223892
version: 1
top level: BBa_J44011
access: public
definition: https://synbiohub.org/public/igem/BBa_S03624/1

BBa_J44000
type: SequenceAnnotation
persistent identity: annotation2223878
display id: annotation2223878
version: 1
top level: BBa_J44011
location: https://synbiohub.org/public/igem/BBa_J44011/annotation2223878/range2223878/1
component: BBa_J44000

https://synbiohub.org/public/igem/BBa_J44011/annotation2223878/range2223878/1
type: Range
persistent identity: range2223878
display id: range2223878
version: 1
direction: direction/reverse
top level: BBa_J44011
start: 1
end: 26
orientation: inline

BBa_S03624
type: SequenceAnnotation
persistent identity: annotation2223892
display id: annotation2223892
version: 1
top level: BBa_J44011
location: https://synbiohub.org/public/igem/BBa_J44011/annotation2223892/range2223892/1
component: BBa_S03624

https://synbiohub.org/public/igem/BBa_J44011/annotation2223892/range2223892/1
type: Range
persistent identity: range2223892
display id: range2223892
version: 1
direction: direction/reverse
top level: BBa_J44011
start: 35
end: 1454
orientation: inline

hixC
type: ComponentDefinition
persistent identity: BBa J44000
display id: BBa_J44000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: hixC binding site for Salmonella typhimurium Hin recombinase
creator: Missouri Western and Davidson Groups, Todd Eckdahl
created: 2006-06-05T11:00:00Z
modified: 2015-08-31T04:08:48Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _71_
m user id: 0
owner id: 606
owning group id: 61
sample status: In stock
status: status/Available
bookmark: true
mutable description: A 26 bp sequence of DNA composed of 12 bp inverted repeats and a 2 bp core that operates in Salmonella paired with a hixR binding site to recombine DNA. A second hix site is required for recombination to occur. The two sites bind Hin recombinase in the formation of an invertasome.
mutable notes: Standard BioBrick prefix and suffix were added to the 26 bp sequence.
mutable provenance: Nanassy and Hughes. 1998. In Vivo Identification of Intermediate Stages of the DNA Inversion Reaction Catlyzed by the Salmonella Hin Recombinase [http://www.genetics.org/cgi/content/abstract/149/4/1649]
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_J44000_sequence/1