//function/regulation/transcriptional
type: Collection
persistent identity: function regulation transcriptional
display id: function_regulation_transcriptional
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
owned by: james
owned by: myers
member: BBa_K082009
member: BBa_K165093
member: BBa_J37033
member: BBa_P0350
member: BBa_K091138
member: BBa_I5000
member: cI TP901
member: BBa_K1486046
member: BBa_I5030
member: BBa_K091203
member: Lov-tetR
member: BBa_K094101
member: BBa_K1520004
member: BBa_K142027
member: BBa_K1031510
member: BBa_P0451
member: BBa_K1031743
member: CspC
member: BBa_K142075
member: BBa_K081020
member: BBa_K1520017
member: BBa_K772004
member: BBa_K1315005
member: BBa_K1486040
member: BBa_K1031016
member: BBa_K1486010
member: BBa_K1031111
member: BBa_K1520518
member: BBa_K1520505
member: BBa_P0452
member: BBa_I1468
member: BBa_K1480005
member: pBAD:CspA
member: BBa_K165022
member: BBa_K1031444
member: BBa_K165050
member: BBa_K165080
member: TetR
member: BBa_I726031
member: BBa_K1520021
member: BBa_K142074
member: BBa_I726061
member: luxr
member: LuxR
member: BBa_J01101
member: BBa_K283032
member: BBa_P0351
member: BBa_I746350
member: BBa_K1031015
member: BBa_K1072023
member: BBa_K772009
member: BBa_K190016
member: BBa_K165082
member: lexA
member: BBa_K165076
member: BBa_K118007
member: BBa_K1520015
member: BBa_K404104
member: rhIR
member: BBa_K404117
member: BBa_K544003
member: BBa_K165089
member: BBa_I714892
member: BBa_K1031917
member: RBS.TetR
member: cI lam
member: BBa_K1520006
member: BBa_K082025
member: BBa_J07009
member: BBa_J24676
member: BBa_K091181
member: BBa_K165086

BBa_K1031011
type: ComponentDefinition
persistent identity: BBa K1031011
display id: BBa_K1031011
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: B0034-RiboJ-φR73
creator: He Shuaixin
created: 2013-09-08T11:00:00Z
modified: 2015-05-08T01:08:47Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1338_
m user id: 0
owner id: 15843
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: phiR-73
mutable notes: phiR73 phage
mutable provenance: lab made
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: phiR73
sequence: https://synbiohub.org/public/igem/BBa_K1031011_sequence/1

phiR73
type: SequenceAnnotation
persistent identity: annotation2353995
display id: annotation2353995
version: 1
top level: BBa_K1031011
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1031011/annotation2353995/range2353995/1

https://synbiohub.org/public/igem/BBa_K1031011/annotation2353995/range2353995/1
type: Range
persistent identity: range2353995
display id: range2353995
version: 1
direction: direction/forward
top level: BBa_K1031011
start: 94
end: 339
orientation: inline

BBa_P0453
type: ComponentDefinition
persistent identity: BBa P0453
display id: BBa_P0453
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PoPS -> cII (p22) [S0155]
creator: Randy Rettberg
created: 2004-04-26T11:00:00Z
modified: 2015-05-08T01:14:11Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0012
component: BBa_C0053
component: BBa_B0010
component: BBa_B0034
sequence annotation: BBa_C0053
sequence annotation: BBa_B0034
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_P0453_sequence/1

BBa_B0012
type: Component
persistent identity: component945052
display id: component945052
version: 1
top level: BBa_P0453
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_C0053
type: Component
persistent identity: component945034
display id: component945034
version: 1
top level: BBa_P0453
access: public
definition: c2 P22

BBa_B0010
type: Component
persistent identity: component945042
display id: component945042
version: 1
top level: BBa_P0453
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0034
type: Component
persistent identity: component945022
display id: component945022
version: 1
top level: BBa_P0453
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_C0053
type: SequenceAnnotation
persistent identity: annotation945034
display id: annotation945034
version: 1
top level: BBa_P0453
location: https://synbiohub.org/public/igem/BBa_P0453/annotation945034/range945034/1
component: BBa_C0053

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type: Range
persistent identity: range945034
display id: range945034
version: 1
direction: direction/forward
top level: BBa_P0453
start: 19
end: 705
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation945022
display id: annotation945022
version: 1
top level: BBa_P0453
location: https://synbiohub.org/public/igem/BBa_P0453/annotation945022/range945022/1
component: BBa_B0034

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type: Range
persistent identity: range945022
display id: range945022
version: 1
direction: direction/forward
top level: BBa_P0453
start: 1
end: 12
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation945042
display id: annotation945042
version: 1
top level: BBa_P0453
location: https://synbiohub.org/public/igem/BBa_P0453/annotation945042/range945042/1
component: BBa_B0010

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type: Range
persistent identity: range945042
display id: range945042
version: 1
direction: direction/forward
top level: BBa_P0453
start: 739
end: 818
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation945052
display id: annotation945052
version: 1
top level: BBa_P0453
location: https://synbiohub.org/public/igem/BBa_P0453/annotation945052/range945052/1
component: BBa_B0012

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type: Range
persistent identity: range945052
display id: range945052
version: 1
direction: direction/forward
top level: BBa_P0453
start: 827
end: 867
orientation: inline

BBa_K142047
type: ComponentDefinition
persistent identity: BBa K142047
display id: BBa_K142047
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: tet-controlled LacI generator with constitutive TetR expression cassette
creator: Julius Rabl
created: 2008-10-28T12:00:00Z
modified: 2015-05-08T01:10:22Z
discontinued: false
dominant: false
group u list: _194_
m user id: 0
owner id: 3254
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This BioBrick contains a tetracyclin-inducable LacI generator, which is followed by a constitutive TetR expression cassette. Since E. coli does not produce TetR, the expression cassette has to be present on the plasmid; otherwise the tet-controlled promotor will be constitutively active.
mutable notes: We decided to put the constitutive expression cassette behind the inducible LacI generator to minimze readthrough from the constitutive expression.
mutable provenance: Assembled from parts K142046 and I739001 by standard cloning techniques.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_K142046
component: BBa_I739001
sequence annotation: BBa_K142046
sequence annotation: BBa_I739001
sequence: https://synbiohub.org/public/igem/BBa_K142047_sequence/1

BBa_K142046
type: Component
persistent identity: component2282708
display id: component2282708
version: 1
top level: BBa_K142047
access: public
definition: https://synbiohub.org/public/igem/BBa_K142046/1

BBa_I739001
type: Component
persistent identity: component2282723
display id: component2282723
version: 1
top level: BBa_K142047
access: public
definition: https://synbiohub.org/public/igem/BBa_I739001/1

BBa_K142046
type: SequenceAnnotation
persistent identity: annotation2282708
display id: annotation2282708
version: 1
top level: BBa_K142047
location: https://synbiohub.org/public/igem/BBa_K142047/annotation2282708/range2282708/1
component: BBa_K142046

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type: Range
persistent identity: range2282708
display id: range2282708
version: 1
direction: direction/reverse
top level: BBa_K142047
start: 1
end: 1370
orientation: inline

BBa_I739001
type: SequenceAnnotation
persistent identity: annotation2282723
display id: annotation2282723
version: 1
top level: BBa_K142047
location: https://synbiohub.org/public/igem/BBa_K142047/annotation2282723/range2282723/1
component: BBa_I739001

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type: Range
persistent identity: range2282723
display id: range2282723
version: 1
direction: direction/reverse
top level: BBa_K142047
start: 1379
end: 2261
orientation: inline

BBa_K1315005
type: ComponentDefinition
persistent identity: BBa K1315005
display id: BBa_K1315005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: The clp gene from Xanthomonas campestris
creator: Dimitrios Michailidis
created: 2014-09-24T11:00:00Z
modified: 2015-05-08T01:09:50Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1690_
m user id: 0
owner id: 21812
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: c-di-GMP Repressed DNA Binding Protein (Clp) Quorum sensing and bacterial cell signalling are mechanisms known to be utilised by bacteria to regulate their virulence. The RpfC-RpfG sensory system has been found to activate the production of virulence factors like extracellular enzymes and polysaccharides in Xanthomonas campestris. It has been also suggested that this system is involved in linking environmental stimuli, including DSF, with pathogenicity in Xanthomonas campestris. DSF is a cis-unsaturated fatty acid from the DSF family. The RpfC-RpfG two-component system is the most well understood one and is present in all Xanthomonads. http://www.ncbi.nlm.nih.gov/pubmed/?term=r+ryan+dsf+family http://www.ncbi.nlm.nih.gov/pubmed/?term=A+two-component+system+involving+an+HD-GYP+domain+protein+links+cell%E2%80%93cell+signalling+to+pathogenicity+gene+expression+in+Xanthomonas+campestris
mutable notes: The sequence had 1 PstI site that was mutated out by site-directed mutagenesis.
mutable provenance: The source of this part is Xanthomonas campestris pv. campestris 8004 genomic DNA which was provided by the Robert Ryan lab in the Molecular Microbiology department of the University of Dundee, UK.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Clp
sequence: https://synbiohub.org/public/igem/BBa_K1315005_sequence/1

Clp
type: SequenceAnnotation
persistent identity: annotation2386527
display id: annotation2386527
version: 1
top level: BBa_K1315005
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1315005/annotation2386527/range2386527/1

https://synbiohub.org/public/igem/BBa_K1315005/annotation2386527/range2386527/1
type: Range
persistent identity: range2386527
display id: range2386527
version: 1
direction: direction/forward
top level: BBa_K1315005
start: 1
end: 696
orientation: inline

BBa_K921002
type: ComponentDefinition
persistent identity: BBa K921002
display id: BBa_K921002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: T7 RNAP + IPTG->PoPs (Mutant III)
creator: Eric Pederson
created: 2012-09-30T11:00:00Z
modified: 2015-05-08T01:13:46Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1186_
m user id: 0
owner id: 12713
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This is a mutant T7 promoter that includes a lacO sequence. This is repressible by the LacI protein and is induced in the presence of IPTG. The promoter sits in the 5' region of the gene of interest and initiates transcription when the cell expresses T7 RNA polymerase. Be sure you are using a cell strain that includes this gene (DE3).
mutable notes: There are three portions to the T7 promoter: the recognition site, the melting box (or TATA box) and the initation site. Mutations in these sequences caused differential behavior from the wild type.
mutable provenance: The part consists of a sequences that resemble a T7 promoter and the lacO sequence found in E. coli.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/T7
role: SO:0001207
sequence annotation: T7 Promoter
sequence annotation: LacO
sequence annotation: Initiation
sequence: https://synbiohub.org/public/igem/BBa_K921002_sequence/1

T7 Promoter
type: SequenceAnnotation
persistent identity: annotation2205316
display id: annotation2205316
version: 1
top level: BBa_K921002
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K921002/annotation2205316/range2205316/1

https://synbiohub.org/public/igem/BBa_K921002/annotation2205316/range2205316/1
type: Range
persistent identity: range2205316
display id: range2205316
version: 1
direction: direction/forward
top level: BBa_K921002
start: 1
end: 20
orientation: inline

LacO
type: SequenceAnnotation
persistent identity: annotation2205317
display id: annotation2205317
version: 1
top level: BBa_K921002
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K921002/annotation2205317/range2205317/1

https://synbiohub.org/public/igem/BBa_K921002/annotation2205317/range2205317/1
type: Range
persistent identity: range2205317
display id: range2205317
version: 1
direction: direction/forward
top level: BBa_K921002
start: 19
end: 47
orientation: inline

Initiation
type: SequenceAnnotation
persistent identity: annotation2205318
display id: annotation2205318
version: 1
top level: BBa_K921002
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K921002/annotation2205318/range2205318/1

https://synbiohub.org/public/igem/BBa_K921002/annotation2205318/range2205318/1
type: Range
persistent identity: range2205318
display id: range2205318
version: 1
direction: direction/forward
top level: BBa_K921002
start: 18
end: 20
orientation: inline

BBa_S04301
type: ComponentDefinition
persistent identity: BBa S04301
display id: BBa_S04301
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: C0079:B0015
creator: UNAM FCiencias-CINVESTAV IRA
created: 2009-10-11T11:00:00Z
modified: 2015-05-08T01:14:38Z
discontinued: false
dominant: false
experience: experience/None
group u list: _9_
m user id: 0
owner id: 1955
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_B0015
component: BBa_C0079
sequence annotation: BBa_C0079
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_S04301_sequence/1

BBa_B0015
type: Component
persistent identity: component2246633
display id: component2246633
version: 1
top level: BBa_S04301
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_C0079
type: Component
persistent identity: component2246626
display id: component2246626
version: 1
top level: BBa_S04301
access: public
definition: lasr

BBa_C0079
type: SequenceAnnotation
persistent identity: annotation2246626
display id: annotation2246626
version: 1
top level: BBa_S04301
location: https://synbiohub.org/public/igem/BBa_S04301/annotation2246626/range2246626/1
component: BBa_C0079

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type: Range
persistent identity: range2246626
display id: range2246626
version: 1
direction: direction/reverse
top level: BBa_S04301
start: 1
end: 781
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2246633
display id: annotation2246633
version: 1
top level: BBa_S04301
location: https://synbiohub.org/public/igem/BBa_S04301/annotation2246633/range2246633/1
component: BBa_B0015

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type: Range
persistent identity: range2246633
display id: range2246633
version: 1
direction: direction/reverse
top level: BBa_S04301
start: 790
end: 918
orientation: inline

BBa_K165100
type: ComponentDefinition
persistent identity: BBa K165100
display id: BBa_K165100
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Gli1 bs + LexA bs + mCYC + LexA repressor (mCherryx2 tagged) on pRS304*
creator: John Szymanski
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:10:57Z
discontinued: false
dominant: true
group u list: _267_
m user id: 0
owner id: 2512
owning group id: 58
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: -
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_K165018
component: BBa_K165002
component: BBa_K165031
component: BBa_K165004
component: BBa_K165009
component: BBa_K165012
component: BBa_K165003
sequence annotation: BBa_K165012
sequence annotation: BBa_K165002
sequence annotation: BBa_K165018
sequence annotation: BBa_K165004
sequence annotation: BBa_K165031
sequence annotation: BBa_K165009
sequence annotation: BBa_K165003
sequence: https://synbiohub.org/public/igem/BBa_K165100_sequence/1

BBa_K165018
type: Component
persistent identity: component1997404
display id: component1997404
version: 1
top level: BBa_K165100
access: public
definition: https://synbiohub.org/public/igem/BBa_K165018/1

BBa_K165002
type: Component
persistent identity: component1997399
display id: component1997399
version: 1
top level: BBa_K165100
access: public
definition: https://synbiohub.org/public/igem/BBa_K165002/1

BBa_K165031
type: Component
persistent identity: component1997398
display id: component1997398
version: 1
top level: BBa_K165100
access: public
definition: https://synbiohub.org/public/igem/BBa_K165031/1

BBa_K165004
type: Component
persistent identity: component1997403
display id: component1997403
version: 1
top level: BBa_K165100
access: public
definition: https://synbiohub.org/public/igem/BBa_K165004/1

BBa_K165004
type: Component
persistent identity: component1997402
display id: component1997402
version: 1
top level: BBa_K165100
access: public
definition: https://synbiohub.org/public/igem/BBa_K165004/1

BBa_K165009
type: Component
persistent identity: component1997400
display id: component1997400
version: 1
top level: BBa_K165100
access: public
definition: https://synbiohub.org/public/igem/BBa_K165009/1

BBa_K165012
type: Component
persistent identity: component1997397
display id: component1997397
version: 1
top level: BBa_K165100
access: public
definition: https://synbiohub.org/public/igem/BBa_K165012/1

BBa_K165003
type: Component
persistent identity: component1997401
display id: component1997401
version: 1
top level: BBa_K165100
access: public
definition: https://synbiohub.org/public/igem/BBa_K165003/1

BBa_K165012
type: SequenceAnnotation
persistent identity: annotation1997397
display id: annotation1997397
version: 1
top level: BBa_K165100
location: https://synbiohub.org/public/igem/BBa_K165100/annotation1997397/range1997397/1
component: BBa_K165012

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type: Range
persistent identity: range1997397
display id: range1997397
version: 1
direction: direction/reverse
top level: BBa_K165100
start: 1
end: 127
orientation: inline

BBa_K165002
type: SequenceAnnotation
persistent identity: annotation1997399
display id: annotation1997399
version: 1
top level: BBa_K165100
location: https://synbiohub.org/public/igem/BBa_K165100/annotation1997399/range1997399/1
component: BBa_K165002

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type: Range
persistent identity: range1997399
display id: range1997399
version: 1
direction: direction/reverse
top level: BBa_K165100
start: 547
end: 564
orientation: inline

BBa_K165018
type: SequenceAnnotation
persistent identity: annotation1997404
display id: annotation1997404
version: 1
top level: BBa_K165100
location: https://synbiohub.org/public/igem/BBa_K165100/annotation1997404/range1997404/1
component: BBa_K165018

https://synbiohub.org/public/igem/BBa_K165100/annotation1997404/range1997404/1
type: Range
persistent identity: range1997404
display id: range1997404
version: 1
direction: direction/reverse
top level: BBa_K165100
start: 5144
end: 5396
orientation: inline

BBa_K165004
type: SequenceAnnotation
persistent identity: annotation1997402
display id: annotation1997402
version: 1
top level: BBa_K165100
location: https://synbiohub.org/public/igem/BBa_K165100/annotation1997402/range1997402/1
component: BBa_K165004

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type: Range
persistent identity: range1997402
display id: range1997402
version: 1
direction: direction/reverse
top level: BBa_K165100
start: 3658
end: 4392
orientation: inline

BBa_K165031
type: SequenceAnnotation
persistent identity: annotation1997398
display id: annotation1997398
version: 1
top level: BBa_K165100
location: https://synbiohub.org/public/igem/BBa_K165100/annotation1997398/range1997398/1
component: BBa_K165031

https://synbiohub.org/public/igem/BBa_K165100/annotation1997398/range1997398/1
type: Range
persistent identity: range1997398
display id: range1997398
version: 1
direction: direction/reverse
top level: BBa_K165100
start: 136
end: 538
orientation: inline

BBa_K165009
type: SequenceAnnotation
persistent identity: annotation1997400
display id: annotation1997400
version: 1
top level: BBa_K165100
location: https://synbiohub.org/public/igem/BBa_K165100/annotation1997400/range1997400/1
component: BBa_K165009

https://synbiohub.org/public/igem/BBa_K165100/annotation1997400/range1997400/1
type: Range
persistent identity: range1997400
display id: range1997400
version: 1
direction: direction/reverse
top level: BBa_K165100
start: 573
end: 830
orientation: inline

BBa_K165004
type: SequenceAnnotation
persistent identity: annotation1997403
display id: annotation1997403
version: 1
top level: BBa_K165100
location: https://synbiohub.org/public/igem/BBa_K165100/annotation1997403/range1997403/1
component: BBa_K165004

https://synbiohub.org/public/igem/BBa_K165100/annotation1997403/range1997403/1
type: Range
persistent identity: range1997403
display id: range1997403
version: 1
direction: direction/reverse
top level: BBa_K165100
start: 4401
end: 5135
orientation: inline

BBa_K165003
type: SequenceAnnotation
persistent identity: annotation1997401
display id: annotation1997401
version: 1
top level: BBa_K165100
location: https://synbiohub.org/public/igem/BBa_K165100/annotation1997401/range1997401/1
component: BBa_K165003

https://synbiohub.org/public/igem/BBa_K165100/annotation1997401/range1997401/1
type: Range
persistent identity: range1997401
display id: range1997401
version: 1
direction: direction/reverse
top level: BBa_K165100
start: 839
end: 3649
orientation: inline

LacI:CspC
type: ComponentDefinition
persistent identity: BBa K538204
display id: BBa_K538204
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pLacI + RBS + CspC
creator: Bas Stringer
created: 2011-09-07T11:00:00Z
modified: 2015-05-08T01:12:37Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _704_
m user id: 0
owner id: 9985
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: -
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_R0010
component: BBa_K538004
component: BBa_B0034
sequence annotation: BBa_R0010
sequence annotation: BBa_B0034
sequence annotation: BBa_K538004
sequence: https://synbiohub.org/public/igem/BBa_K538204_sequence/1

BBa_R0010
type: Component
persistent identity: component2134072
display id: component2134072
version: 1
top level: LacI:CspC
access: public
definition: https://synbiohub.org/public/igem/BBa_R0010/1

BBa_K538004
type: Component
persistent identity: component2134086
display id: component2134086
version: 1
top level: LacI:CspC
access: public
definition: CspC

BBa_B0034
type: Component
persistent identity: component2134080
display id: component2134080
version: 1
top level: LacI:CspC
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation2134072
display id: annotation2134072
version: 1
top level: LacI:CspC
location: https://synbiohub.org/public/igem/BBa_K538204/annotation2134072/range2134072/1
component: BBa_R0010

https://synbiohub.org/public/igem/BBa_K538204/annotation2134072/range2134072/1
type: Range
persistent identity: range2134072
display id: range2134072
version: 1
direction: direction/forward
top level: LacI:CspC
start: 1
end: 200
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2134080
display id: annotation2134080
version: 1
top level: LacI:CspC
location: https://synbiohub.org/public/igem/BBa_K538204/annotation2134080/range2134080/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K538204/annotation2134080/range2134080/1
type: Range
persistent identity: range2134080
display id: range2134080
version: 1
direction: direction/reverse
top level: LacI:CspC
start: 209
end: 220
orientation: inline

BBa_K538004
type: SequenceAnnotation
persistent identity: annotation2134086
display id: annotation2134086
version: 1
top level: LacI:CspC
location: https://synbiohub.org/public/igem/BBa_K538204/annotation2134086/range2134086/1
component: BBa_K538004

https://synbiohub.org/public/igem/BBa_K538204/annotation2134086/range2134086/1
type: Range
persistent identity: range2134086
display id: range2134086
version: 1
direction: direction/reverse
top level: LacI:CspC
start: 227
end: 421
orientation: inline

BBa_K1031410
type: ComponentDefinition
persistent identity: BBa K1031410
display id: BBa_K1031410
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: HcaR-Terminator
creator: He Shuaixin
created: 2013-09-08T11:00:00Z
modified: 2015-05-08T01:08:47Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1338_
m user id: 0
owner id: 15843
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: HcaR-TT
mutable notes: molecular cloning
mutable provenance: PCR genome E.coli
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: HcaR
sequence annotation: BBa_B0015
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K1031410_sequence/1

HcaR
type: SequenceAnnotation
persistent identity: annotation2357451
display id: annotation2357451
version: 1
top level: BBa_K1031410
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1031410/annotation2357451/range2357451/1

https://synbiohub.org/public/igem/BBa_K1031410/annotation2357451/range2357451/1
type: Range
persistent identity: range2357451
display id: range2357451
version: 1
direction: direction/forward
top level: BBa_K1031410
start: 19
end: 909
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2357452
display id: annotation2357452
version: 1
top level: BBa_K1031410
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1031410/annotation2357452/range2357452/1

https://synbiohub.org/public/igem/BBa_K1031410/annotation2357452/range2357452/1
type: Range
persistent identity: range2357452
display id: range2357452
version: 1
direction: direction/forward
top level: BBa_K1031410
start: 918
end: 1046
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2357450
display id: annotation2357450
version: 1
top level: BBa_K1031410
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1031410/annotation2357450/range2357450/1

https://synbiohub.org/public/igem/BBa_K1031410/annotation2357450/range2357450/1
type: Range
persistent identity: range2357450
display id: range2357450
version: 1
direction: direction/forward
top level: BBa_K1031410
start: 1
end: 12
orientation: inline

BBa_K1031510
type: ComponentDefinition
persistent identity: BBa K1031510
display id: BBa_K1031510
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: HpaR-Terminator
creator: He Shuaixin
created: 2013-09-08T11:00:00Z
modified: 2015-05-08T01:08:47Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1338_
m user id: 0
owner id: 15843
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: HpaR-TT
mutable notes: molecular cloning
mutable provenance: PCR genome E.coli
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: BBa_B0034
sequence annotation: BBa_B0015
sequence annotation: HpaR
sequence: https://synbiohub.org/public/igem/BBa_K1031510_sequence/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2357469
display id: annotation2357469
version: 1
top level: BBa_K1031510
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1031510/annotation2357469/range2357469/1

https://synbiohub.org/public/igem/BBa_K1031510/annotation2357469/range2357469/1
type: Range
persistent identity: range2357469
display id: range2357469
version: 1
direction: direction/forward
top level: BBa_K1031510
start: 1
end: 12
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2357473
display id: annotation2357473
version: 1
top level: BBa_K1031510
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1031510/annotation2357473/range2357473/1

https://synbiohub.org/public/igem/BBa_K1031510/annotation2357473/range2357473/1
type: Range
persistent identity: range2357473
display id: range2357473
version: 1
direction: direction/forward
top level: BBa_K1031510
start: 474
end: 602
orientation: inline

HpaR
type: SequenceAnnotation
persistent identity: annotation2357472
display id: annotation2357472
version: 1
top level: BBa_K1031510
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1031510/annotation2357472/range2357472/1

https://synbiohub.org/public/igem/BBa_K1031510/annotation2357472/range2357472/1
type: Range
persistent identity: range2357472
display id: range2357472
version: 1
direction: direction/forward
top level: BBa_K1031510
start: 19
end: 465
orientation: inline

BBa_I732081
type: ComponentDefinition
persistent identity: BBa I732081
display id: BBa_I732081
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Tet repressor (RBS+ LVA- TERM-)
creator: Zhan Jian
created: 2007-08-02T11:00:00Z
modified: 2015-08-31T04:07:57Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_I732080
component: BBa_B0034
sequence annotation: BBa_B0034
sequence annotation: BBa_I732080
sequence: https://synbiohub.org/public/igem/BBa_I732081_sequence/1

BBa_I732080
type: Component
persistent identity: component1940760
display id: component1940760
version: 1
top level: BBa_I732081
access: public
definition: https://synbiohub.org/public/igem/BBa_I732080/1

BBa_B0034
type: Component
persistent identity: component1940758
display id: component1940758
version: 1
top level: BBa_I732081
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1940758
display id: annotation1940758
version: 1
top level: BBa_I732081
location: https://synbiohub.org/public/igem/BBa_I732081/annotation1940758/range1940758/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I732081/annotation1940758/range1940758/1
type: Range
persistent identity: range1940758
display id: range1940758
version: 1
direction: direction/reverse
top level: BBa_I732081
start: 1
end: 12
orientation: inline

BBa_I732080
type: SequenceAnnotation
persistent identity: annotation1940760
display id: annotation1940760
version: 1
top level: BBa_I732081
location: https://synbiohub.org/public/igem/BBa_I732081/annotation1940760/range1940760/1
component: BBa_I732080

https://synbiohub.org/public/igem/BBa_I732081/annotation1940760/range1940760/1
type: Range
persistent identity: range1940760
display id: range1940760
version: 1
direction: direction/reverse
top level: BBa_I732081
start: 19
end: 645
orientation: inline

BBa_K1520017
type: ComponentDefinition
persistent identity: BBa K1520017
display id: BBa_K1520017
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Pcons2-rbs-luxR-Ter-Prlux-rbs-lacI-Ter-Plac-rbs-rfp-Ter
creator: yue wu
created: 2014-09-21T11:00:00Z
modified: 2015-05-08T01:10:49Z
discontinued: false
dominant: false
group u list: _1902_
m user id: 0
owner id: 21318
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Plasmid1 in detector: Pcons2-rbs-luxR-Ter-Prlux-rbs-lacI-Ter-Plac-rbs-rfp-Ter It is a high level detection plasmid. It produces rfp when AHL is not so much.
mutable notes: None
mutable provenance: Pcons2,luxR, Ter, Prlux, lacI, Ter, Plac, rfp, Ter
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1520005
component: BBa_K1520016
sequence annotation: BBa_K1520005
sequence annotation: BBa_K1520016
sequence: https://synbiohub.org/public/igem/BBa_K1520017_sequence/1

BBa_K1520005
type: Component
persistent identity: component2385052
display id: component2385052
version: 1
top level: BBa_K1520017
access: public
definition: https://synbiohub.org/public/igem/BBa_K1520005/1

BBa_K1520016
type: Component
persistent identity: component2385042
display id: component2385042
version: 1
top level: BBa_K1520017
access: public
definition: BBa_K1520016

BBa_K1520005
type: SequenceAnnotation
persistent identity: annotation2385052
display id: annotation2385052
version: 1
top level: BBa_K1520017
location: https://synbiohub.org/public/igem/BBa_K1520017/annotation2385052/range2385052/1
component: BBa_K1520005

https://synbiohub.org/public/igem/BBa_K1520017/annotation2385052/range2385052/1
type: Range
persistent identity: range2385052
display id: range2385052
version: 1
direction: direction/reverse
top level: BBa_K1520017
start: 2367
end: 3443
orientation: inline

BBa_K1520016
type: SequenceAnnotation
persistent identity: annotation2385042
display id: annotation2385042
version: 1
top level: BBa_K1520017
location: https://synbiohub.org/public/igem/BBa_K1520017/annotation2385042/range2385042/1
component: BBa_K1520016

https://synbiohub.org/public/igem/BBa_K1520017/annotation2385042/range2385042/1
type: Range
persistent identity: range2385042
display id: range2385042
version: 1
direction: direction/reverse
top level: BBa_K1520017
start: 1
end: 2358
orientation: inline

BBa_K864203
type: ComponentDefinition
persistent identity: BBa K864203
display id: BBa_K864203
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LexA3 super-repressor
creator: Erik Lundin
created: 2012-09-24T11:00:00Z
modified: 2015-05-08T01:13:37Z
discontinued: false
dominant: false
group u list: _1124_
m user id: 0
owner id: 9827
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: lexA3
mutable notes: lexA3 is lexA (BBa_K864201) with a G85D mutation (G>A basepair change at position 254)
mutable provenance: BBa_K864201
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: G>A
sequence annotation: lexA3
sequence annotation: G95D
sequence: https://synbiohub.org/public/igem/BBa_K864203_sequence/1

G>A
type: SequenceAnnotation
persistent identity: annotation2197173
display id: annotation2197173
version: 1
top level: BBa_K864203
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K864203/annotation2197173/range2197173/1

https://synbiohub.org/public/igem/BBa_K864203/annotation2197173/range2197173/1
type: Range
persistent identity: range2197173
display id: range2197173
version: 1
direction: direction/forward
top level: BBa_K864203
start: 254
end: 254
orientation: inline

lexA3
type: SequenceAnnotation
persistent identity: annotation2197171
display id: annotation2197171
version: 1
top level: BBa_K864203
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K864203/annotation2197171/range2197171/1

https://synbiohub.org/public/igem/BBa_K864203/annotation2197171/range2197171/1
type: Range
persistent identity: range2197171
display id: range2197171
version: 1
direction: direction/forward
top level: BBa_K864203
start: 1
end: 612
orientation: inline

G95D
type: SequenceAnnotation
persistent identity: annotation2197172
display id: annotation2197172
version: 1
top level: BBa_K864203
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K864203/annotation2197172/range2197172/1

https://synbiohub.org/public/igem/BBa_K864203/annotation2197172/range2197172/1
type: Range
persistent identity: range2197172
display id: range2197172
version: 1
direction: direction/forward
top level: BBa_K864203
start: 253
end: 255
orientation: inline

BBa_K1486041
type: ComponentDefinition
persistent identity: BBa K1486041
display id: BBa_K1486041
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: sfGFPC + CpxR
creator: Ione Pla Puigsubir??
created: 2014-09-26T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: To edit
mutable notes: To edit
mutable provenance: To edit
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_I0500
component: BBa_K1486039
component: BBa_B0034
component: BBa_K1486000
component: BBa_K1486037
sequence annotation: BBa_B0034
sequence annotation: BBa_I0500
sequence annotation: BBa_K1486039
sequence annotation: BBa_K1486000
sequence annotation: BBa_K1486037
sequence: https://synbiohub.org/public/igem/BBa_K1486041_sequence/1

BBa_I0500
type: Component
persistent identity: component2387209
display id: component2387209
version: 1
top level: BBa_K1486041
access: public
definition: https://synbiohub.org/public/igem/BBa_I0500/1

BBa_K1486039
type: Component
persistent identity: component2387212
display id: component2387212
version: 1
top level: BBa_K1486041
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486039/1

BBa_B0034
type: Component
persistent identity: component2387211
display id: component2387211
version: 1
top level: BBa_K1486041
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K1486000
type: Component
persistent identity: component2387217
display id: component2387217
version: 1
top level: BBa_K1486041
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486000/1

BBa_K1486037
type: Component
persistent identity: component2387213
display id: component2387213
version: 1
top level: BBa_K1486041
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486037/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2387211
display id: annotation2387211
version: 1
top level: BBa_K1486041
location: https://synbiohub.org/public/igem/BBa_K1486041/annotation2387211/range2387211/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1486041/annotation2387211/range2387211/1
type: Range
persistent identity: range2387211
display id: range2387211
version: 1
direction: direction/reverse
top level: BBa_K1486041
start: 1211
end: 1222
orientation: inline

BBa_I0500
type: SequenceAnnotation
persistent identity: annotation2387209
display id: annotation2387209
version: 1
top level: BBa_K1486041
location: https://synbiohub.org/public/igem/BBa_K1486041/annotation2387209/range2387209/1
component: BBa_I0500

https://synbiohub.org/public/igem/BBa_K1486041/annotation2387209/range2387209/1
type: Range
persistent identity: range2387209
display id: range2387209
version: 1
direction: direction/reverse
top level: BBa_K1486041
start: 1
end: 1210
orientation: inline

BBa_K1486039
type: SequenceAnnotation
persistent identity: annotation2387212
display id: annotation2387212
version: 1
top level: BBa_K1486041
location: https://synbiohub.org/public/igem/BBa_K1486041/annotation2387212/range2387212/1
component: BBa_K1486039

https://synbiohub.org/public/igem/BBa_K1486041/annotation2387212/range2387212/1
type: Range
persistent identity: range2387212
display id: range2387212
version: 1
direction: direction/reverse
top level: BBa_K1486041
start: 1223
end: 1294
orientation: inline

BBa_K1486000
type: SequenceAnnotation
persistent identity: annotation2387217
display id: annotation2387217
version: 1
top level: BBa_K1486041
location: https://synbiohub.org/public/igem/BBa_K1486041/annotation2387217/range2387217/1
component: BBa_K1486000

https://synbiohub.org/public/igem/BBa_K1486041/annotation2387217/range2387217/1
type: Range
persistent identity: range2387217
display id: range2387217
version: 1
direction: direction/reverse
top level: BBa_K1486041
start: 1334
end: 2035
orientation: inline

BBa_K1486037
type: SequenceAnnotation
persistent identity: annotation2387213
display id: annotation2387213
version: 1
top level: BBa_K1486041
location: https://synbiohub.org/public/igem/BBa_K1486041/annotation2387213/range2387213/1
component: BBa_K1486037

https://synbiohub.org/public/igem/BBa_K1486041/annotation2387213/range2387213/1
type: Range
persistent identity: range2387213
display id: range2387213
version: 1
direction: direction/reverse
top level: BBa_K1486041
start: 1295
end: 1333
orientation: inline

rhIR
type: ComponentDefinition
persistent identity: BBa C0171
display id: BBa_C0171
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: rhlR repressor/activator from P. aeruginosa PA3477 (no LVA)
creator: jcbraff
created: 2004-05-26T11:00:00Z
modified: 2015-08-31T04:07:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_1_
m user id: 0
owner id: 61
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: same as C0071 except no LVA tag
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_C0171_sequence/1

BBa_P0412
type: ComponentDefinition
persistent identity: BBa P0412
display id: BBa_P0412
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PoPS -> LacI [S0150]
creator: Randy Rettberg
created: 2004-04-26T11:00:00Z
modified: 2015-05-08T01:14:10Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0012
component: BBa_B0010
component: BBa_C0012
component: BBa_B0034
sequence annotation: BBa_B0012
sequence annotation: BBa_B0034
sequence annotation: BBa_B0010
sequence annotation: BBa_C0012
sequence: https://synbiohub.org/public/igem/BBa_P0412_sequence/1

BBa_B0012
type: Component
persistent identity: component944854
display id: component944854
version: 1
top level: BBa_P0412
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0010
type: Component
persistent identity: component944844
display id: component944844
version: 1
top level: BBa_P0412
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_C0012
type: Component
persistent identity: component944837
display id: component944837
version: 1
top level: BBa_P0412
access: public
definition: lacI

BBa_B0034
type: Component
persistent identity: component944827
display id: component944827
version: 1
top level: BBa_P0412
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation944854
display id: annotation944854
version: 1
top level: BBa_P0412
location: https://synbiohub.org/public/igem/BBa_P0412/annotation944854/range944854/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_P0412/annotation944854/range944854/1
type: Range
persistent identity: range944854
display id: range944854
version: 1
direction: direction/forward
top level: BBa_P0412
start: 1268
end: 1308
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation944827
display id: annotation944827
version: 1
top level: BBa_P0412
location: https://synbiohub.org/public/igem/BBa_P0412/annotation944827/range944827/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_P0412/annotation944827/range944827/1
type: Range
persistent identity: range944827
display id: range944827
version: 1
direction: direction/forward
top level: BBa_P0412
start: 1
end: 12
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation944844
display id: annotation944844
version: 1
top level: BBa_P0412
location: https://synbiohub.org/public/igem/BBa_P0412/annotation944844/range944844/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_P0412/annotation944844/range944844/1
type: Range
persistent identity: range944844
display id: range944844
version: 1
direction: direction/forward
top level: BBa_P0412
start: 1180
end: 1259
orientation: inline

BBa_C0012
type: SequenceAnnotation
persistent identity: annotation944837
display id: annotation944837
version: 1
top level: BBa_P0412
location: https://synbiohub.org/public/igem/BBa_P0412/annotation944837/range944837/1
component: BBa_C0012

https://synbiohub.org/public/igem/BBa_P0412/annotation944837/range944837/1
type: Range
persistent identity: range944837
display id: range944837
version: 1
direction: direction/forward
top level: BBa_P0412
start: 19
end: 1146
orientation: inline

BBa_K116626
type: ComponentDefinition
persistent identity: BBa K116626
display id: BBa_K116626
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: BBa_B0032 + LuxR + BBa_B0015
creator: Jesse Wu
created: 2008-10-30T12:00:00Z
modified: 2015-05-08T01:09:33Z
discontinued: false
dominant: false
experience: experience/None
group u list: _210_
m user id: 0
owner id: 2749
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: RBS + LuxR + T.
mutable notes: none
mutable provenance: ligation
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K116616
component: BBa_B0015
sequence annotation: BBa_K116616
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_K116626_sequence/1

BBa_K116616
type: Component
persistent identity: component2249466
display id: component2249466
version: 1
top level: BBa_K116626
access: public
definition: https://synbiohub.org/public/igem/BBa_K116616/1

BBa_B0015
type: Component
persistent identity: component2249473
display id: component2249473
version: 1
top level: BBa_K116626
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_K116616
type: SequenceAnnotation
persistent identity: annotation2249466
display id: annotation2249466
version: 1
top level: BBa_K116626
location: https://synbiohub.org/public/igem/BBa_K116626/annotation2249466/range2249466/1
component: BBa_K116616

https://synbiohub.org/public/igem/BBa_K116626/annotation2249466/range2249466/1
type: Range
persistent identity: range2249466
display id: range2249466
version: 1
direction: direction/reverse
top level: BBa_K116626
start: 1
end: 800
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2249473
display id: annotation2249473
version: 1
top level: BBa_K116626
location: https://synbiohub.org/public/igem/BBa_K116626/annotation2249473/range2249473/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K116626/annotation2249473/range2249473/1
type: Range
persistent identity: range2249473
display id: range2249473
version: 1
direction: direction/reverse
top level: BBa_K116626
start: 809
end: 937
orientation: inline

pBAD:CspC
type: ComponentDefinition
persistent identity: BBa K538304
display id: BBa_K538304
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pBAD + RBS + CspC
creator: Bas Stringer
created: 2011-09-07T11:00:00Z
modified: 2015-05-08T01:12:37Z
discontinued: false
dominant: false
experience: experience/None
group u list: _704_
m user id: 0
owner id: 9985
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: -
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0034
component: BBa_K538004
component: BBa_K206000
sequence annotation: BBa_B0034
sequence annotation: BBa_K538004
sequence annotation: BBa_K206000
sequence: https://synbiohub.org/public/igem/BBa_K538304_sequence/1

BBa_B0034
type: Component
persistent identity: component2134103
display id: component2134103
version: 1
top level: pBAD:CspC
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K538004
type: Component
persistent identity: component2134109
display id: component2134109
version: 1
top level: pBAD:CspC
access: public
definition: CspC

BBa_K206000
type: Component
persistent identity: component2134101
display id: component2134101
version: 1
top level: pBAD:CspC
access: public
definition: https://synbiohub.org/public/igem/BBa_K206000/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2134103
display id: annotation2134103
version: 1
top level: pBAD:CspC
location: https://synbiohub.org/public/igem/BBa_K538304/annotation2134103/range2134103/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K538304/annotation2134103/range2134103/1
type: Range
persistent identity: range2134103
display id: range2134103
version: 1
direction: direction/reverse
top level: pBAD:CspC
start: 139
end: 150
orientation: inline

BBa_K538004
type: SequenceAnnotation
persistent identity: annotation2134109
display id: annotation2134109
version: 1
top level: pBAD:CspC
location: https://synbiohub.org/public/igem/BBa_K538304/annotation2134109/range2134109/1
component: BBa_K538004

https://synbiohub.org/public/igem/BBa_K538304/annotation2134109/range2134109/1
type: Range
persistent identity: range2134109
display id: range2134109
version: 1
direction: direction/reverse
top level: pBAD:CspC
start: 157
end: 351
orientation: inline

BBa_K206000
type: SequenceAnnotation
persistent identity: annotation2134101
display id: annotation2134101
version: 1
top level: pBAD:CspC
location: https://synbiohub.org/public/igem/BBa_K538304/annotation2134101/range2134101/1
component: BBa_K206000

https://synbiohub.org/public/igem/BBa_K538304/annotation2134101/range2134101/1
type: Range
persistent identity: range2134101
display id: range2134101
version: 1
direction: direction/reverse
top level: pBAD:CspC
start: 1
end: 130
orientation: inline

BBa_K1480004
type: ComponentDefinition
persistent identity: BBa K1480004
display id: BBa_K1480004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TetR generator (Promoter J23106 )
creator: Jesus Gilberto Rodriguez Ceja
created: 2014-10-13T11:00:00Z
modified: 2015-05-08T01:10:40Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1860_
m user id: 0
owner id: 12878
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is a TetR protein generator regulated by the constitutive promoter J23106. The protein has a LVA tag for degradation.
mutable notes: Based on parts of registry.
mutable provenance: It was assembled using assembly 10.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0010
component: BBa_J23106
component: BBa_B0034
component: BBa_B0012
component: BBa_C0040
sequence annotation: BBa_J23106
sequence annotation: BBa_B0034
sequence annotation: BBa_C0040
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_K1480004_sequence/1

BBa_B0010
type: Component
persistent identity: component2419548
display id: component2419548
version: 1
top level: BBa_K1480004
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_J23106
type: Component
persistent identity: component2419541
display id: component2419541
version: 1
top level: BBa_K1480004
access: public
definition: https://synbiohub.org/public/igem/BBa_J23106/1

BBa_B0034
type: Component
persistent identity: component2419543
display id: component2419543
version: 1
top level: BBa_K1480004
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0012
type: Component
persistent identity: component2419550
display id: component2419550
version: 1
top level: BBa_K1480004
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_C0040
type: Component
persistent identity: component2419547
display id: component2419547
version: 1
top level: BBa_K1480004
access: public
definition: tetR

BBa_J23106
type: SequenceAnnotation
persistent identity: annotation2419541
display id: annotation2419541
version: 1
top level: BBa_K1480004
location: https://synbiohub.org/public/igem/BBa_K1480004/annotation2419541/range2419541/1
component: BBa_J23106

https://synbiohub.org/public/igem/BBa_K1480004/annotation2419541/range2419541/1
type: Range
persistent identity: range2419541
display id: range2419541
version: 1
direction: direction/reverse
top level: BBa_K1480004
start: 1
end: 35
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2419543
display id: annotation2419543
version: 1
top level: BBa_K1480004
location: https://synbiohub.org/public/igem/BBa_K1480004/annotation2419543/range2419543/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1480004/annotation2419543/range2419543/1
type: Range
persistent identity: range2419543
display id: range2419543
version: 1
direction: direction/reverse
top level: BBa_K1480004
start: 44
end: 55
orientation: inline

BBa_C0040
type: SequenceAnnotation
persistent identity: annotation2419547
display id: annotation2419547
version: 1
top level: BBa_K1480004
location: https://synbiohub.org/public/igem/BBa_K1480004/annotation2419547/range2419547/1
component: BBa_C0040

https://synbiohub.org/public/igem/BBa_K1480004/annotation2419547/range2419547/1
type: Range
persistent identity: range2419547
display id: range2419547
version: 1
direction: direction/reverse
top level: BBa_K1480004
start: 62
end: 746
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2419548
display id: annotation2419548
version: 1
top level: BBa_K1480004
location: https://synbiohub.org/public/igem/BBa_K1480004/annotation2419548/range2419548/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K1480004/annotation2419548/range2419548/1
type: Range
persistent identity: range2419548
display id: range2419548
version: 1
direction: direction/forward
top level: BBa_K1480004
start: 755
end: 834
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2419550
display id: annotation2419550
version: 1
top level: BBa_K1480004
location: https://synbiohub.org/public/igem/BBa_K1480004/annotation2419550/range2419550/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K1480004/annotation2419550/range2419550/1
type: Range
persistent identity: range2419550
display id: range2419550
version: 1
direction: direction/forward
top level: BBa_K1480004
start: 843
end: 883
orientation: inline

BBa_K404104
type: ComponentDefinition
persistent identity: BBa K404104
display id: BBa_K404104
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: p5-TATAless promoter
creator: Freiburg Bioware 2010
created: 2010-08-27T11:00:00Z
modified: 2015-05-08T01:12:23Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 7049
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: p5TATAless
sequence: https://synbiohub.org/public/igem/BBa_K404104_sequence/1

p5TATAless
type: SequenceAnnotation
persistent identity: annotation2094942
display id: annotation2094942
version: 1
top level: BBa_K404104
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K404104/annotation2094942/range2094942/1

https://synbiohub.org/public/igem/BBa_K404104/annotation2094942/range2094942/1
type: Range
persistent identity: range2094942
display id: range2094942
version: 1
direction: direction/forward
top level: BBa_K404104
start: 1
end: 164
orientation: inline

BBa_K292006
type: ComponentDefinition
persistent identity: BBa K292006
display id: BBa_K292006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Strong RBS + LacI repressor + double terminator
creator: David Charoy
created: 2009-10-18T11:00:00Z
modified: 2015-05-08T01:11:49Z
discontinued: false
dominant: true
group u list: _394_
m user id: 0
owner id: 5212
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part contains a strong ribosome binding site, the LacI repressor and a double terminator (BBa_B0030 + BBa_C0012 + BBa_B0014). This brick comes from the BBa_K292005. The LacI repressor represses pLac promoter from the E.coli lactose operon. So LacI repressor and this bio-brick are really reliable to use when we want to control a gene expression by using pLac promoter. In fact when LacI is not expressed, pLac is activated, and when there is an expression of LacI, pLac is inhibited. We can easily design a system to induce the transcription of LacI by using another operon as the tetracycline operon and creates a failed feedback mechanism for pLac inhibition and so, for gene expression inhibition. The LacI (BBa_K292006) already contains a strong RBS and a double terminator, so this brick is ready to use! History: In E.Coli, pLac is a part of the lactose operon. LacI is constantly expressed and represses the pLac. In the presence of Lactose, LacI is inhibited and pLac is active and allows the transcription of beta-galactosidase. Lactose can be substituted by IPTG (Isopropyl β-D-1-thiogalactopyranoside).
mutable notes: N/A
mutable provenance: Part:BBa_B0030, Designed by Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. Group: NCBS, Prather Lab Part:BBa_C0012, Designed by Grace Kenney, Daniel Shen, Neelaksh Varshney, Samantha Sutton Group: Registry Part:BBa_B0014, Designed by Reshma Shetty Group: Registry (2003-07-16)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0030
component: BBa_C0012
component: BBa_B0014
sequence annotation: BBa_B0014
sequence annotation: BBa_C0012
sequence annotation: BBa_B0030
sequence: https://synbiohub.org/public/igem/BBa_K292006_sequence/1

BBa_B0030
type: Component
persistent identity: component2249929
display id: component2249929
version: 1
top level: BBa_K292006
access: public
definition: https://synbiohub.org/public/igem/BBa_B0030/1

BBa_C0012
type: Component
persistent identity: component2249932
display id: component2249932
version: 1
top level: BBa_K292006
access: public
definition: lacI

BBa_B0014
type: Component
persistent identity: component2249941
display id: component2249941
version: 1
top level: BBa_K292006
access: public
definition: https://synbiohub.org/public/igem/BBa_B0014/1

BBa_B0014
type: SequenceAnnotation
persistent identity: annotation2249941
display id: annotation2249941
version: 1
top level: BBa_K292006
location: https://synbiohub.org/public/igem/BBa_K292006/annotation2249941/range2249941/1
component: BBa_B0014

https://synbiohub.org/public/igem/BBa_K292006/annotation2249941/range2249941/1
type: Range
persistent identity: range2249941
display id: range2249941
version: 1
direction: direction/reverse
top level: BBa_K292006
start: 1183
end: 1277
orientation: inline

BBa_C0012
type: SequenceAnnotation
persistent identity: annotation2249932
display id: annotation2249932
version: 1
top level: BBa_K292006
location: https://synbiohub.org/public/igem/BBa_K292006/annotation2249932/range2249932/1
component: BBa_C0012

https://synbiohub.org/public/igem/BBa_K292006/annotation2249932/range2249932/1
type: Range
persistent identity: range2249932
display id: range2249932
version: 1
direction: direction/forward
top level: BBa_K292006
start: 22
end: 1149
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation2249929
display id: annotation2249929
version: 1
top level: BBa_K292006
location: https://synbiohub.org/public/igem/BBa_K292006/annotation2249929/range2249929/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_K292006/annotation2249929/range2249929/1
type: Range
persistent identity: range2249929
display id: range2249929
version: 1
direction: direction/forward
top level: BBa_K292006
start: 1
end: 15
orientation: inline

BBa_K190017
type: ComponentDefinition
persistent identity: BBa K190017
display id: BBa_K190017
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Copper Promoter (CueR regulated)
creator: Michael Verhoeven
created: 2009-07-14T11:00:00Z
modified: 2015-05-08T01:11:15Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _306_
m user id: 0
owner id: 4144
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Promoter sequence with recognition site for CueR transcriptional regulator protein.
mutable notes: None
mutable provenance: E.coli TOP10
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -35 and -10 region
sequence annotation: CueR binding site
sequence: https://synbiohub.org/public/igem/BBa_K190017_sequence/1

-35 and -10 region
type: SequenceAnnotation
persistent identity: annotation2011505
display id: annotation2011505
version: 1
top level: BBa_K190017
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K190017/annotation2011505/range2011505/1

https://synbiohub.org/public/igem/BBa_K190017/annotation2011505/range2011505/1
type: Range
persistent identity: range2011505
display id: range2011505
version: 1
direction: direction/forward
top level: BBa_K190017
start: 1
end: 43
orientation: inline

CueR binding site
type: SequenceAnnotation
persistent identity: annotation2011506
display id: annotation2011506
version: 1
top level: BBa_K190017
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K190017/annotation2011506/range2011506/1

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type: Range
persistent identity: range2011506
display id: range2011506
version: 1
direction: direction/forward
top level: BBa_K190017
start: 3
end: 28
orientation: inline

BBa_P0440
type: ComponentDefinition
persistent identity: BBa P0440
display id: BBa_P0440
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PoPS -> TetR [S0151]
creator: Randy Rettberg
created: 2004-04-26T11:00:00Z
modified: 2015-05-08T01:14:10Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_C0040
component: BBa_B0034
component: BBa_B0010
component: BBa_B0012
sequence annotation: BBa_B0010
sequence annotation: BBa_B0034
sequence annotation: BBa_C0040
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_P0440_sequence/1

BBa_C0040
type: Component
persistent identity: component944896
display id: component944896
version: 1
top level: BBa_P0440
access: public
definition: tetR

BBa_B0034
type: Component
persistent identity: component944886
display id: component944886
version: 1
top level: BBa_P0440
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0010
type: Component
persistent identity: component944902
display id: component944902
version: 1
top level: BBa_P0440
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0012
type: Component
persistent identity: component944912
display id: component944912
version: 1
top level: BBa_P0440
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation944902
display id: annotation944902
version: 1
top level: BBa_P0440
location: https://synbiohub.org/public/igem/BBa_P0440/annotation944902/range944902/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_P0440/annotation944902/range944902/1
type: Range
persistent identity: range944902
display id: range944902
version: 1
direction: direction/forward
top level: BBa_P0440
start: 712
end: 791
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation944886
display id: annotation944886
version: 1
top level: BBa_P0440
location: https://synbiohub.org/public/igem/BBa_P0440/annotation944886/range944886/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_P0440/annotation944886/range944886/1
type: Range
persistent identity: range944886
display id: range944886
version: 1
direction: direction/forward
top level: BBa_P0440
start: 1
end: 12
orientation: inline

BBa_C0040
type: SequenceAnnotation
persistent identity: annotation944896
display id: annotation944896
version: 1
top level: BBa_P0440
location: https://synbiohub.org/public/igem/BBa_P0440/annotation944896/range944896/1
component: BBa_C0040

https://synbiohub.org/public/igem/BBa_P0440/annotation944896/range944896/1
type: Range
persistent identity: range944896
display id: range944896
version: 1
direction: direction/forward
top level: BBa_P0440
start: 19
end: 678
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation944912
display id: annotation944912
version: 1
top level: BBa_P0440
location: https://synbiohub.org/public/igem/BBa_P0440/annotation944912/range944912/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_P0440/annotation944912/range944912/1
type: Range
persistent identity: range944912
display id: range944912
version: 1
direction: direction/forward
top level: BBa_P0440
start: 800
end: 840
orientation: inline

BBa_K1861050
type: ComponentDefinition
persistent identity: BBa K1861050
display id: BBa_K1861050
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Fusion protein of TALE1 and a SpyTag in an expression cassette
creator: Vladyslav Vyshnevskyi
created: 2015-09-17T11:00:00Z
modified: 2015-09-23T04:16:18Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2291_
m user id: 25348
owner id: 25348
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Coming soon.
mutable notes: Coming soon.
mutable provenance: Coming soon.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K392003
component: BBa_K1861101
component: BBa_K1861501
component: BBa_K1861104
component: BBa_K1159201
component: BBa_K1861102
sequence annotation: BBa_K392003
sequence annotation: BBa_K1159201
sequence annotation: BBa_K1861104
sequence annotation: BBa_K1861101
sequence annotation: BBa_K1861501
sequence annotation: BBa_K1861102
sequence: https://synbiohub.org/public/igem/BBa_K1861050_sequence/1

BBa_K392003
type: Component
persistent identity: component2474418
display id: component2474418
version: 1
top level: BBa_K1861050
access: public
definition: https://synbiohub.org/public/igem/BBa_K392003/1

BBa_K1861101
type: Component
persistent identity: component2474411
display id: component2474411
version: 1
top level: BBa_K1861050
access: public
definition: https://synbiohub.org/public/igem/BBa_K1861101/1

BBa_K1861501
type: Component
persistent identity: component2474413
display id: component2474413
version: 1
top level: BBa_K1861050
access: public
definition: https://synbiohub.org/public/igem/BBa_K1861501/1

BBa_K1861104
type: Component
persistent identity: component2474417
display id: component2474417
version: 1
top level: BBa_K1861050
access: public
definition: https://synbiohub.org/public/igem/BBa_K1861104/1

BBa_K1159201
type: Component
persistent identity: component2474416
display id: component2474416
version: 1
top level: BBa_K1861050
access: public
definition: https://synbiohub.org/public/igem/BBa_K1159201/1

BBa_K1861102
type: Component
persistent identity: component2474412
display id: component2474412
version: 1
top level: BBa_K1861050
access: public
definition: https://synbiohub.org/public/igem/BBa_K1861102/1

BBa_K392003
type: SequenceAnnotation
persistent identity: annotation2474418
display id: annotation2474418
version: 1
top level: BBa_K1861050
location: https://synbiohub.org/public/igem/BBa_K1861050/annotation2474418/range2474418/1
component: BBa_K392003

https://synbiohub.org/public/igem/BBa_K1861050/annotation2474418/range2474418/1
type: Range
persistent identity: range2474418
display id: range2474418
version: 1
direction: direction/reverse
top level: BBa_K1861050
start: 2525
end: 2653
orientation: inline

BBa_K1159201
type: SequenceAnnotation
persistent identity: annotation2474416
display id: annotation2474416
version: 1
top level: BBa_K1861050
location: https://synbiohub.org/public/igem/BBa_K1861050/annotation2474416/range2474416/1
component: BBa_K1159201

https://synbiohub.org/public/igem/BBa_K1861050/annotation2474416/range2474416/1
type: Range
persistent identity: range2474416
display id: range2474416
version: 1
direction: direction/reverse
top level: BBa_K1861050
start: 2477
end: 2515
orientation: inline

BBa_K1861104
type: SequenceAnnotation
persistent identity: annotation2474417
display id: annotation2474417
version: 1
top level: BBa_K1861050
location: https://synbiohub.org/public/igem/BBa_K1861050/annotation2474417/range2474417/1
component: BBa_K1861104

https://synbiohub.org/public/igem/BBa_K1861050/annotation2474417/range2474417/1
type: Range
persistent identity: range2474417
display id: range2474417
version: 1
direction: direction/reverse
top level: BBa_K1861050
start: 2516
end: 2524
orientation: inline

BBa_K1861101
type: SequenceAnnotation
persistent identity: annotation2474411
display id: annotation2474411
version: 1
top level: BBa_K1861050
location: https://synbiohub.org/public/igem/BBa_K1861050/annotation2474411/range2474411/1
component: BBa_K1861101

https://synbiohub.org/public/igem/BBa_K1861050/annotation2474411/range2474411/1
type: Range
persistent identity: range2474411
display id: range2474411
version: 1
direction: direction/reverse
top level: BBa_K1861050
start: 1
end: 238
orientation: inline

BBa_K1861501
type: SequenceAnnotation
persistent identity: annotation2474413
display id: annotation2474413
version: 1
top level: BBa_K1861050
location: https://synbiohub.org/public/igem/BBa_K1861050/annotation2474413/range2474413/1
component: BBa_K1861501

https://synbiohub.org/public/igem/BBa_K1861050/annotation2474413/range2474413/1
type: Range
persistent identity: range2474413
display id: range2474413
version: 1
direction: direction/reverse
top level: BBa_K1861050
start: 266
end: 2476
orientation: inline

BBa_K1861102
type: SequenceAnnotation
persistent identity: annotation2474412
display id: annotation2474412
version: 1
top level: BBa_K1861050
location: https://synbiohub.org/public/igem/BBa_K1861050/annotation2474412/range2474412/1
component: BBa_K1861102

https://synbiohub.org/public/igem/BBa_K1861050/annotation2474412/range2474412/1
type: Range
persistent identity: range2474412
display id: range2474412
version: 1
direction: direction/reverse
top level: BBa_K1861050
start: 239
end: 265
orientation: inline

BBa_K1031211
type: ComponentDefinition
persistent identity: BBa K1031211
display id: BBa_K1031211
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Pr-DmpR
creator: He Shuaixin
created: 2013-10-20T11:00:00Z
modified: 2015-05-08T01:08:47Z
discontinued: false
dominant: false
group u list: _1338_
m user id: 0
owner id: 15843
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Pr-DmpR::pSB1C3
mutable notes: molecular cloning
mutable provenance: Pseudomonas.putida
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Pr
sequence annotation: DmpR
sequence: https://synbiohub.org/public/igem/BBa_K1031211_sequence/1

Pr
type: SequenceAnnotation
persistent identity: annotation2369617
display id: annotation2369617
version: 1
top level: BBa_K1031211
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1031211/annotation2369617/range2369617/1

https://synbiohub.org/public/igem/BBa_K1031211/annotation2369617/range2369617/1
type: Range
persistent identity: range2369617
display id: range2369617
version: 1
direction: direction/forward
top level: BBa_K1031211
start: 1
end: 74
orientation: inline

DmpR
type: SequenceAnnotation
persistent identity: annotation2369618
display id: annotation2369618
version: 1
top level: BBa_K1031211
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1031211/annotation2369618/range2369618/1

https://synbiohub.org/public/igem/BBa_K1031211/annotation2369618/range2369618/1
type: Range
persistent identity: range2369618
display id: range2369618
version: 1
direction: direction/forward
top level: BBa_K1031211
start: 75
end: 1766
orientation: inline

BBa_K1031442
type: ComponentDefinition
persistent identity: BBa K1031442
display id: BBa_K1031442
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: J23109-HcaR
creator: He Shuaixin
created: 2013-09-08T11:00:00Z
modified: 2015-05-08T01:08:47Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1338_
m user id: 0
owner id: 15843
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: 109-HcaR-TT
mutable notes: molecular cloning
mutable provenance: PCR genome E.coli
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: BBa_J23109
sequence annotation: HcaR
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K1031442_sequence/1

BBa_J23109
type: SequenceAnnotation
persistent identity: annotation2357456
display id: annotation2357456
version: 1
top level: BBa_K1031442
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1031442/annotation2357456/range2357456/1

https://synbiohub.org/public/igem/BBa_K1031442/annotation2357456/range2357456/1
type: Range
persistent identity: range2357456
display id: range2357456
version: 1
direction: direction/forward
top level: BBa_K1031442
start: 1
end: 35
orientation: inline

HcaR
type: SequenceAnnotation
persistent identity: annotation2357458
display id: annotation2357458
version: 1
top level: BBa_K1031442
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1031442/annotation2357458/range2357458/1

https://synbiohub.org/public/igem/BBa_K1031442/annotation2357458/range2357458/1
type: Range
persistent identity: range2357458
display id: range2357458
version: 1
direction: direction/forward
top level: BBa_K1031442
start: 62
end: 952
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2357457
display id: annotation2357457
version: 1
top level: BBa_K1031442
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1031442/annotation2357457/range2357457/1

https://synbiohub.org/public/igem/BBa_K1031442/annotation2357457/range2357457/1
type: Range
persistent identity: range2357457
display id: range2357457
version: 1
direction: direction/forward
top level: BBa_K1031442
start: 44
end: 55
orientation: inline

BBa_K137131
type: ComponentDefinition
persistent identity: BBa K137131
display id: BBa_K137131
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Lactose inducible lysis cassette expression
creator: Josh Michener
created: 2008-10-22T11:00:00Z
modified: 2015-05-08T01:10:11Z
discontinued: false
dominant: false
experience: experience/None
group u list: _187_
m user id: 0
owner id: 253
owning group id: 6
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Temp
mutable notes: Constitutive LacI expression ensures tight repression of lysis.
mutable provenance: Lysis cassette is cloned from Lambda Zap II genomic DNA.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_S04114
component: BBa_S04106
sequence annotation: BBa_S04106
sequence annotation: BBa_S04114
sequence: https://synbiohub.org/public/igem/BBa_K137131_sequence/1

BBa_S04114
type: Component
persistent identity: component2276506
display id: component2276506
version: 1
top level: BBa_K137131
access: public
definition: https://synbiohub.org/public/igem/BBa_S04114/1

BBa_S04106
type: Component
persistent identity: component2276497
display id: component2276497
version: 1
top level: BBa_K137131
access: public
definition: https://synbiohub.org/public/igem/BBa_S04106/1

BBa_S04106
type: SequenceAnnotation
persistent identity: annotation2276497
display id: annotation2276497
version: 1
top level: BBa_K137131
location: https://synbiohub.org/public/igem/BBa_K137131/annotation2276497/range2276497/1
component: BBa_S04106

https://synbiohub.org/public/igem/BBa_K137131/annotation2276497/range2276497/1
type: Range
persistent identity: range2276497
display id: range2276497
version: 1
direction: direction/reverse
top level: BBa_K137131
start: 1
end: 1462
orientation: inline

BBa_S04114
type: SequenceAnnotation
persistent identity: annotation2276506
display id: annotation2276506
version: 1
top level: BBa_K137131
location: https://synbiohub.org/public/igem/BBa_K137131/annotation2276506/range2276506/1
component: BBa_S04114

https://synbiohub.org/public/igem/BBa_K137131/annotation2276506/range2276506/1
type: Range
persistent identity: range2276506
display id: range2276506
version: 1
direction: direction/reverse
top level: BBa_K137131
start: 1471
end: 2923
orientation: inline

BBa_K1031013
type: ComponentDefinition
persistent identity: BBa K1031013
display id: BBa_K1031013
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: NahR-Psal-RBS-RiboJ-CI
creator: He Shuaixin
created: 2013-09-08T11:00:00Z
modified: 2015-05-08T01:08:47Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1338_
m user id: 0
owner id: 15843
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: NahR Psel Ribo_J CI
mutable notes: Pseudomonas putida, phiR73, E.coli
mutable provenance: lab made
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: promoter
sequence annotation: cI
sequence annotation: nahR
sequence: https://synbiohub.org/public/igem/BBa_K1031013_sequence/1

promoter
type: SequenceAnnotation
persistent identity: annotation2353997
display id: annotation2353997
version: 1
top level: BBa_K1031013
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1031013/annotation2353997/range2353997/1

https://synbiohub.org/public/igem/BBa_K1031013/annotation2353997/range2353997/1
type: Range
persistent identity: range2353997
display id: range2353997
version: 1
direction: direction/forward
top level: BBa_K1031013
start: 904
end: 1050
orientation: inline

cI
type: SequenceAnnotation
persistent identity: annotation2353998
display id: annotation2353998
version: 1
top level: BBa_K1031013
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1031013/annotation2353998/range2353998/1

https://synbiohub.org/public/igem/BBa_K1031013/annotation2353998/range2353998/1
type: Range
persistent identity: range2353998
display id: range2353998
version: 1
direction: direction/forward
top level: BBa_K1031013
start: 1156
end: 1872
orientation: inline

nahR
type: SequenceAnnotation
persistent identity: annotation2353996
display id: annotation2353996
version: 1
top level: BBa_K1031013
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1031013/annotation2353996/range2353996/1

https://synbiohub.org/public/igem/BBa_K1031013/annotation2353996/range2353996/1
type: Range
persistent identity: range2353996
display id: range2353996
version: 1
direction: direction/reverse
top level: BBa_K1031013
start: 1
end: 903
orientation: inline

BBa_I746352
type: ComponentDefinition
persistent identity: BBa I746352
display id: BBa_I746352
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: delta activator from phiR73 phage
creator: Stefan Milde
created: 2007-09-11T11:00:00Z
modified: 2015-08-31T04:08:04Z
discontinued: false
dominant: false
experience: experience/None
group u list: _116_
m user id: 0
owner id: 2122
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: The delta activator taken from phiR73 phage acts on a class of inducible promoters (parts I746360 to I746365), inducing their activity to varying degrees. The part sequence does already contain a ribosome binding site (B0034)!
mutable notes: The part does contain a RBS (B0034) already.
mutable provenance: plasmid DNA supplied by Prof. Richard Calendar, University of California.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
sequence annotation: phiR73 delta
sequence annotation: B0034
sequence annotation: silent mutation to remove PstI site
sequence: https://synbiohub.org/public/igem/BBa_I746352_sequence/1

phiR73 delta
type: SequenceAnnotation
persistent identity: annotation1943873
display id: annotation1943873
version: 1
top level: BBa_I746352
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_I746352/annotation1943873/range1943873/1

https://synbiohub.org/public/igem/BBa_I746352/annotation1943873/range1943873/1
type: Range
persistent identity: range1943873
display id: range1943873
version: 1
direction: direction/forward
top level: BBa_I746352
start: 22
end: 22
orientation: inline

phiR73 delta
type: SequenceAnnotation
persistent identity: annotation1943872
display id: annotation1943872
version: 1
top level: BBa_I746352
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_I746352/annotation1943872/range1943872/1

https://synbiohub.org/public/igem/BBa_I746352/annotation1943872/range1943872/1
type: Range
persistent identity: range1943872
display id: range1943872
version: 1
direction: direction/forward
top level: BBa_I746352
start: 22
end: 264
orientation: inline

B0034
type: SequenceAnnotation
persistent identity: annotation1943871
display id: annotation1943871
version: 1
top level: BBa_I746352
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_I746352/annotation1943871/range1943871/1

https://synbiohub.org/public/igem/BBa_I746352/annotation1943871/range1943871/1
type: Range
persistent identity: range1943871
display id: range1943871
version: 1
direction: direction/forward
top level: BBa_I746352
start: 1
end: 12
orientation: inline

silent mutation to remove PstI site
type: SequenceAnnotation
persistent identity: annotation1943874
display id: annotation1943874
version: 1
top level: BBa_I746352
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_I746352/annotation1943874/range1943874/1

https://synbiohub.org/public/igem/BBa_I746352/annotation1943874/range1943874/1
type: Range
persistent identity: range1943874
display id: range1943874
version: 1
direction: direction/forward
top level: BBa_I746352
start: 102
end: 102
orientation: inline

BBa_K082009
type: ComponentDefinition
persistent identity: BBa K082009
display id: BBa_K082009
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: B0034-C0051
creator: Yanting Xue , He Shen
created: 2008-10-27T12:00:00Z
modified: 2015-05-08T01:08:35Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _228_
m user id: 0
owner id: 1560
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: none
mutable notes: none
mutable provenance: none
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_B0034
component: BBa_C0051
sequence annotation: BBa_B0034
sequence annotation: BBa_C0051
sequence: https://synbiohub.org/public/igem/BBa_K082009_sequence/1

BBa_B0034
type: Component
persistent identity: component2244053
display id: component2244053
version: 1
top level: BBa_K082009
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_C0051
type: Component
persistent identity: component2244057
display id: component2244057
version: 1
top level: BBa_K082009
access: public
definition: cI lam

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2244053
display id: annotation2244053
version: 1
top level: BBa_K082009
location: https://synbiohub.org/public/igem/BBa_K082009/annotation2244053/range2244053/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K082009/annotation2244053/range2244053/1
type: Range
persistent identity: range2244053
display id: range2244053
version: 1
direction: direction/reverse
top level: BBa_K082009
start: 1
end: 12
orientation: inline

BBa_C0051
type: SequenceAnnotation
persistent identity: annotation2244057
display id: annotation2244057
version: 1
top level: BBa_K082009
location: https://synbiohub.org/public/igem/BBa_K082009/annotation2244057/range2244057/1
component: BBa_C0051

https://synbiohub.org/public/igem/BBa_K082009/annotation2244057/range2244057/1
type: Range
persistent identity: range2244057
display id: range2244057
version: 1
direction: direction/reverse
top level: BBa_K082009
start: 19
end: 793
orientation: inline

BBa_K1520513
type: ComponentDefinition
persistent identity: BBa K1520513
display id: BBa_K1520513
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: MC7-rbs-tetR-Ter-PtetO-rbs-rfp-Ter-Plac-rbs-tetR-Ter-Pcons2-rbs-lacI-Ter
creator: yue wu
created: 2014-09-22T11:00:00Z
modified: 2015-05-08T01:10:49Z
discontinued: false
dominant: false
group u list: _1902_
m user id: 0
owner id: 21318
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: MC7-rbs-tetR-Ter-PtetO-rbs-rfp-Ter-Plac-rbs-tetR-Ter-Pcons2-rbs-lacI-Ter It is a test of our suicide circuit for microcystin.
mutable notes: None
mutable provenance: MC17, tetR, PtetO, rfp, Plac, Pcons2, lacI, Ter
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1520029
component: BBa_K1520001
component: BBa_K1520026
component: BBa_K1520505
sequence annotation: BBa_K1520026
sequence annotation: BBa_K1520029
sequence annotation: BBa_K1520001
sequence annotation: BBa_K1520505
sequence: https://synbiohub.org/public/igem/BBa_K1520513_sequence/1

BBa_K1520029
type: Component
persistent identity: component2385604
display id: component2385604
version: 1
top level: BBa_K1520513
access: public
definition: https://synbiohub.org/public/igem/BBa_K1520029/1

BBa_K1520001
type: Component
persistent identity: component2385600
display id: component2385600
version: 1
top level: BBa_K1520513
access: public
definition: https://synbiohub.org/public/igem/BBa_K1520001/1

BBa_K1520026
type: Component
persistent identity: component2385632
display id: component2385632
version: 1
top level: BBa_K1520513
access: public
definition: https://synbiohub.org/public/igem/BBa_K1520026/1

BBa_K1520505
type: Component
persistent identity: component2385603
display id: component2385603
version: 1
top level: BBa_K1520513
access: public
definition: BBa_K1520505

BBa_K1520026
type: SequenceAnnotation
persistent identity: annotation2385632
display id: annotation2385632
version: 1
top level: BBa_K1520513
location: https://synbiohub.org/public/igem/BBa_K1520513/annotation2385632/range2385632/1
component: BBa_K1520026

https://synbiohub.org/public/igem/BBa_K1520513/annotation2385632/range2385632/1
type: Range
persistent identity: range2385632
display id: range2385632
version: 1
direction: direction/reverse
top level: BBa_K1520513
start: 990
end: 4335
orientation: inline

BBa_K1520029
type: SequenceAnnotation
persistent identity: annotation2385604
display id: annotation2385604
version: 1
top level: BBa_K1520513
location: https://synbiohub.org/public/igem/BBa_K1520513/annotation2385604/range2385604/1
component: BBa_K1520029

https://synbiohub.org/public/igem/BBa_K1520513/annotation2385604/range2385604/1
type: Range
persistent identity: range2385604
display id: range2385604
version: 1
direction: direction/reverse
top level: BBa_K1520513
start: 853
end: 981
orientation: inline

BBa_K1520001
type: SequenceAnnotation
persistent identity: annotation2385600
display id: annotation2385600
version: 1
top level: BBa_K1520513
location: https://synbiohub.org/public/igem/BBa_K1520513/annotation2385600/range2385600/1
component: BBa_K1520001

https://synbiohub.org/public/igem/BBa_K1520513/annotation2385600/range2385600/1
type: Range
persistent identity: range2385600
display id: range2385600
version: 1
direction: direction/reverse
top level: BBa_K1520513
start: 1
end: 133
orientation: inline

BBa_K1520505
type: SequenceAnnotation
persistent identity: annotation2385603
display id: annotation2385603
version: 1
top level: BBa_K1520513
location: https://synbiohub.org/public/igem/BBa_K1520513/annotation2385603/range2385603/1
component: BBa_K1520505

https://synbiohub.org/public/igem/BBa_K1520513/annotation2385603/range2385603/1
type: Range
persistent identity: range2385603
display id: range2385603
version: 1
direction: direction/reverse
top level: BBa_K1520513
start: 142
end: 844
orientation: inline

AnIn
type: ComponentDefinition
persistent identity: BBa K772007
display id: BBa_K772007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: GFP - LasR anchor-inducer protein complex
creator: Mustafa Elitok
created: 2012-09-25T11:00:00Z
modified: 2015-05-08T01:13:15Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1024_
m user id: 0
owner id: 9573
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This part contains a green fluorescent protein (GFP) as a non-reactive chemical compound (anchor) in order to neutralize the active reactive fixed to it, an inducer regulatory protein (LasR is responsible to induce pLas promoter in the presence of 6HSLO3 which is a chemical compound sythesized by an enzyme that LasI gene codes.) and a TEV protease cleavage site between them to assemble these two proteins. BBa_772001 and BBa_K772002 are assembled to be used as the trigger of detection in the whole system which ends with the intracellular transfection (or activation) of TEV protease. After the activation, it is planned that TEV protease will cleave this anchor-inducer protein complex to release free the reporter. In conclusion, in this system, the inducer would activate a transcription pathway on the targeted inducible gene or the reporter would indicate that there is a change in the whole system.
mutable notes: Cleavage site, LasR and GFP sequences are gathered from Registry.
mutable provenance: Note that there is an RBS sequence just before the protein sequence in order to eliminate the need of addition of it.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
sequence annotation: GFP
sequence annotation: RBS
sequence annotation: LasR
sequence annotation: TEV cleavage site
sequence annotation: Double Terminator
sequence annotation: Anchor - Inducer
sequence: https://synbiohub.org/public/igem/BBa_K772007_sequence/1

GFP
type: SequenceAnnotation
persistent identity: annotation2203899
display id: annotation2203899
version: 1
top level: AnIn
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K772007/annotation2203899/range2203899/1

https://synbiohub.org/public/igem/BBa_K772007/annotation2203899/range2203899/1
type: Range
persistent identity: range2203899
display id: range2203899
version: 1
direction: direction/forward
top level: AnIn
start: 21
end: 741
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2203898
display id: annotation2203898
version: 1
top level: AnIn
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K772007/annotation2203898/range2203898/1

https://synbiohub.org/public/igem/BBa_K772007/annotation2203898/range2203898/1
type: Range
persistent identity: range2203898
display id: range2203898
version: 1
direction: direction/forward
top level: AnIn
start: 1
end: 20
orientation: inline

LasR
type: SequenceAnnotation
persistent identity: annotation2203901
display id: annotation2203901
version: 1
top level: AnIn
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K772007/annotation2203901/range2203901/1

https://synbiohub.org/public/igem/BBa_K772007/annotation2203901/range2203901/1
type: Range
persistent identity: range2203901
display id: range2203901
version: 1
direction: direction/forward
top level: AnIn
start: 880
end: 1636
orientation: inline

TEV cleavage site
type: SequenceAnnotation
persistent identity: annotation2203900
display id: annotation2203900
version: 1
top level: AnIn
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K772007/annotation2203900/range2203900/1

https://synbiohub.org/public/igem/BBa_K772007/annotation2203900/range2203900/1
type: Range
persistent identity: range2203900
display id: range2203900
version: 1
direction: direction/forward
top level: AnIn
start: 782
end: 871
orientation: inline

Double Terminator
type: SequenceAnnotation
persistent identity: annotation2203909
display id: annotation2203909
version: 1
top level: AnIn
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K772007/annotation2203909/range2203909/1

https://synbiohub.org/public/igem/BBa_K772007/annotation2203909/range2203909/1
type: Range
persistent identity: range2203909
display id: range2203909
version: 1
direction: direction/forward
top level: AnIn
start: 1636
end: 1720
orientation: inline

Anchor - Inducer
type: SequenceAnnotation
persistent identity: annotation2203902
display id: annotation2203902
version: 1
top level: AnIn
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K772007/annotation2203902/range2203902/1

https://synbiohub.org/public/igem/BBa_K772007/annotation2203902/range2203902/1
type: Range
persistent identity: range2203902
display id: range2203902
version: 1
direction: direction/forward
top level: AnIn
start: 21
end: 1636
orientation: inline

BBa_K1861060
type: ComponentDefinition
persistent identity: BBa K1861060
display id: BBa_K1861060
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Fusion protein of TALE2 and a SpyTag in an expression cassette
creator: Vladyslav Vyshnevskyi
created: 2015-09-17T11:00:00Z
modified: 2015-09-23T04:10:41Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2291_
m user id: 25348
owner id: 25348
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Why can't I edit these descriptions?
mutable notes: Coming soon, if I am allowed to edit this.
mutable provenance: It keeps saying I have no permission to edit this.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1861102
component: BBa_K1861101
component: BBa_K392003
component: BBa_K1861601
component: BBa_K1861104
component: BBa_K1159201
sequence annotation: BBa_K1861104
sequence annotation: BBa_K1159201
sequence annotation: BBa_K1861101
sequence annotation: BBa_K1861102
sequence annotation: BBa_K392003
sequence annotation: BBa_K1861601
sequence: https://synbiohub.org/public/igem/BBa_K1861060_sequence/1

BBa_K1861102
type: Component
persistent identity: component2475041
display id: component2475041
version: 1
top level: BBa_K1861060
access: public
definition: https://synbiohub.org/public/igem/BBa_K1861102/1

BBa_K1861101
type: Component
persistent identity: component2475040
display id: component2475040
version: 1
top level: BBa_K1861060
access: public
definition: https://synbiohub.org/public/igem/BBa_K1861101/1

BBa_K392003
type: Component
persistent identity: component2475047
display id: component2475047
version: 1
top level: BBa_K1861060
access: public
definition: https://synbiohub.org/public/igem/BBa_K392003/1

BBa_K1861601
type: Component
persistent identity: component2475042
display id: component2475042
version: 1
top level: BBa_K1861060
access: public
definition: https://synbiohub.org/public/igem/BBa_K1861601/1

BBa_K1861104
type: Component
persistent identity: component2475046
display id: component2475046
version: 1
top level: BBa_K1861060
access: public
definition: https://synbiohub.org/public/igem/BBa_K1861104/1

BBa_K1159201
type: Component
persistent identity: component2475045
display id: component2475045
version: 1
top level: BBa_K1861060
access: public
definition: https://synbiohub.org/public/igem/BBa_K1159201/1

BBa_K1861104
type: SequenceAnnotation
persistent identity: annotation2475046
display id: annotation2475046
version: 1
top level: BBa_K1861060
location: https://synbiohub.org/public/igem/BBa_K1861060/annotation2475046/range2475046/1
component: BBa_K1861104

https://synbiohub.org/public/igem/BBa_K1861060/annotation2475046/range2475046/1
type: Range
persistent identity: range2475046
display id: range2475046
version: 1
direction: direction/reverse
top level: BBa_K1861060
start: 2515
end: 2523
orientation: inline

BBa_K1159201
type: SequenceAnnotation
persistent identity: annotation2475045
display id: annotation2475045
version: 1
top level: BBa_K1861060
location: https://synbiohub.org/public/igem/BBa_K1861060/annotation2475045/range2475045/1
component: BBa_K1159201

https://synbiohub.org/public/igem/BBa_K1861060/annotation2475045/range2475045/1
type: Range
persistent identity: range2475045
display id: range2475045
version: 1
direction: direction/reverse
top level: BBa_K1861060
start: 2476
end: 2514
orientation: inline

BBa_K1861101
type: SequenceAnnotation
persistent identity: annotation2475040
display id: annotation2475040
version: 1
top level: BBa_K1861060
location: https://synbiohub.org/public/igem/BBa_K1861060/annotation2475040/range2475040/1
component: BBa_K1861101

https://synbiohub.org/public/igem/BBa_K1861060/annotation2475040/range2475040/1
type: Range
persistent identity: range2475040
display id: range2475040
version: 1
direction: direction/reverse
top level: BBa_K1861060
start: 1
end: 238
orientation: inline

BBa_K1861102
type: SequenceAnnotation
persistent identity: annotation2475041
display id: annotation2475041
version: 1
top level: BBa_K1861060
location: https://synbiohub.org/public/igem/BBa_K1861060/annotation2475041/range2475041/1
component: BBa_K1861102

https://synbiohub.org/public/igem/BBa_K1861060/annotation2475041/range2475041/1
type: Range
persistent identity: range2475041
display id: range2475041
version: 1
direction: direction/reverse
top level: BBa_K1861060
start: 239
end: 265
orientation: inline

BBa_K392003
type: SequenceAnnotation
persistent identity: annotation2475047
display id: annotation2475047
version: 1
top level: BBa_K1861060
location: https://synbiohub.org/public/igem/BBa_K1861060/annotation2475047/range2475047/1
component: BBa_K392003

https://synbiohub.org/public/igem/BBa_K1861060/annotation2475047/range2475047/1
type: Range
persistent identity: range2475047
display id: range2475047
version: 1
direction: direction/reverse
top level: BBa_K1861060
start: 2524
end: 2652
orientation: inline

BBa_K1861601
type: SequenceAnnotation
persistent identity: annotation2475042
display id: annotation2475042
version: 1
top level: BBa_K1861060
location: https://synbiohub.org/public/igem/BBa_K1861060/annotation2475042/range2475042/1
component: BBa_K1861601

https://synbiohub.org/public/igem/BBa_K1861060/annotation2475042/range2475042/1
type: Range
persistent identity: range2475042
display id: range2475042
version: 1
direction: direction/reverse
top level: BBa_K1861060
start: 266
end: 2475
orientation: inline

BBa_K091181
type: ComponentDefinition
persistent identity: BBa K091181
display id: BBa_K091181
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pBad-RBS-tetR-TT-placI/cI-RBS-lasR-TT
creator: Robert Cool
created: 2008-08-04T11:00:00Z
modified: 2015-05-08T01:08:38Z
discontinued: false
dominant: false
experience: experience/None
group u list: _191_
m user id: 0
owner id: 3080
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This part was made by ligating K091145 to the 5' end of S03966.
mutable notes: no special consideration
mutable provenance: These parts can be found in the registry.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_K091145
component: BBa_S03966
sequence annotation: BBa_K091145
sequence annotation: BBa_S03966
sequence: https://synbiohub.org/public/igem/BBa_K091181_sequence/1

BBa_K091145
type: Component
persistent identity: component2281646
display id: component2281646
version: 1
top level: BBa_K091181
access: public
definition: https://synbiohub.org/public/igem/BBa_K091145/1

BBa_S03966
type: Component
persistent identity: component2281668
display id: component2281668
version: 1
top level: BBa_K091181
access: public
definition: https://synbiohub.org/public/igem/BBa_S03966/1

BBa_K091145
type: SequenceAnnotation
persistent identity: annotation2281646
display id: annotation2281646
version: 1
top level: BBa_K091181
location: https://synbiohub.org/public/igem/BBa_K091181/annotation2281646/range2281646/1
component: BBa_K091145

https://synbiohub.org/public/igem/BBa_K091181/annotation2281646/range2281646/1
type: Range
persistent identity: range2281646
display id: range2281646
version: 1
direction: direction/reverse
top level: BBa_K091181
start: 1
end: 978
orientation: inline

BBa_S03966
type: SequenceAnnotation
persistent identity: annotation2281668
display id: annotation2281668
version: 1
top level: BBa_K091181
location: https://synbiohub.org/public/igem/BBa_K091181/annotation2281668/range2281668/1
component: BBa_S03966

https://synbiohub.org/public/igem/BBa_K091181/annotation2281668/range2281668/1
type: Range
persistent identity: range2281668
display id: range2281668
version: 1
direction: direction/reverse
top level: BBa_K091181
start: 987
end: 1985
orientation: inline

BBa_K165090
type: ComponentDefinition
persistent identity: BBa K165090
display id: BBa_K165090
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Gli1 bs + LexA bs + mCYC + LexA repressor (mCherryx2 tagged) on pRS306
creator: John Szymanski
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:10:57Z
discontinued: false
dominant: true
experience: experience/None
group u list: _267_
m user id: 0
owner id: 2512
owning group id: 58
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: -
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_K165031
component: BBa_K165012
component: BBa_K165018
component: BBa_K165004
component: BBa_K165003
component: BBa_K165002
component: BBa_K165009
sequence annotation: BBa_K165031
sequence annotation: BBa_K165004
sequence annotation: BBa_K165018
sequence annotation: BBa_K165012
sequence annotation: BBa_K165002
sequence annotation: BBa_K165009
sequence annotation: BBa_K165003
sequence: https://synbiohub.org/public/igem/BBa_K165090_sequence/1

BBa_K165031
type: Component
persistent identity: component1997223
display id: component1997223
version: 1
top level: BBa_K165090
access: public
definition: https://synbiohub.org/public/igem/BBa_K165031/1

BBa_K165012
type: Component
persistent identity: component1997222
display id: component1997222
version: 1
top level: BBa_K165090
access: public
definition: https://synbiohub.org/public/igem/BBa_K165012/1

BBa_K165018
type: Component
persistent identity: component1997229
display id: component1997229
version: 1
top level: BBa_K165090
access: public
definition: https://synbiohub.org/public/igem/BBa_K165018/1

BBa_K165004
type: Component
persistent identity: component1997228
display id: component1997228
version: 1
top level: BBa_K165090
access: public
definition: https://synbiohub.org/public/igem/BBa_K165004/1

BBa_K165003
type: Component
persistent identity: component1997226
display id: component1997226
version: 1
top level: BBa_K165090
access: public
definition: https://synbiohub.org/public/igem/BBa_K165003/1

BBa_K165004
type: Component
persistent identity: component1997227
display id: component1997227
version: 1
top level: BBa_K165090
access: public
definition: https://synbiohub.org/public/igem/BBa_K165004/1

BBa_K165002
type: Component
persistent identity: component1997224
display id: component1997224
version: 1
top level: BBa_K165090
access: public
definition: https://synbiohub.org/public/igem/BBa_K165002/1

BBa_K165009
type: Component
persistent identity: component1997225
display id: component1997225
version: 1
top level: BBa_K165090
access: public
definition: https://synbiohub.org/public/igem/BBa_K165009/1

BBa_K165031
type: SequenceAnnotation
persistent identity: annotation1997223
display id: annotation1997223
version: 1
top level: BBa_K165090
location: https://synbiohub.org/public/igem/BBa_K165090/annotation1997223/range1997223/1
component: BBa_K165031

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type: Range
persistent identity: range1997223
display id: range1997223
version: 1
direction: direction/reverse
top level: BBa_K165090
start: 136
end: 538
orientation: inline

BBa_K165004
type: SequenceAnnotation
persistent identity: annotation1997227
display id: annotation1997227
version: 1
top level: BBa_K165090
location: https://synbiohub.org/public/igem/BBa_K165090/annotation1997227/range1997227/1
component: BBa_K165004

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type: Range
persistent identity: range1997227
display id: range1997227
version: 1
direction: direction/reverse
top level: BBa_K165090
start: 3658
end: 4392
orientation: inline

BBa_K165018
type: SequenceAnnotation
persistent identity: annotation1997229
display id: annotation1997229
version: 1
top level: BBa_K165090
location: https://synbiohub.org/public/igem/BBa_K165090/annotation1997229/range1997229/1
component: BBa_K165018

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type: Range
persistent identity: range1997229
display id: range1997229
version: 1
direction: direction/reverse
top level: BBa_K165090
start: 5144
end: 5396
orientation: inline

BBa_K165012
type: SequenceAnnotation
persistent identity: annotation1997222
display id: annotation1997222
version: 1
top level: BBa_K165090
location: https://synbiohub.org/public/igem/BBa_K165090/annotation1997222/range1997222/1
component: BBa_K165012

https://synbiohub.org/public/igem/BBa_K165090/annotation1997222/range1997222/1
type: Range
persistent identity: range1997222
display id: range1997222
version: 1
direction: direction/reverse
top level: BBa_K165090
start: 1
end: 127
orientation: inline

BBa_K165002
type: SequenceAnnotation
persistent identity: annotation1997224
display id: annotation1997224
version: 1
top level: BBa_K165090
location: https://synbiohub.org/public/igem/BBa_K165090/annotation1997224/range1997224/1
component: BBa_K165002

https://synbiohub.org/public/igem/BBa_K165090/annotation1997224/range1997224/1
type: Range
persistent identity: range1997224
display id: range1997224
version: 1
direction: direction/reverse
top level: BBa_K165090
start: 547
end: 564
orientation: inline

BBa_K165009
type: SequenceAnnotation
persistent identity: annotation1997225
display id: annotation1997225
version: 1
top level: BBa_K165090
location: https://synbiohub.org/public/igem/BBa_K165090/annotation1997225/range1997225/1
component: BBa_K165009

https://synbiohub.org/public/igem/BBa_K165090/annotation1997225/range1997225/1
type: Range
persistent identity: range1997225
display id: range1997225
version: 1
direction: direction/reverse
top level: BBa_K165090
start: 573
end: 830
orientation: inline

BBa_K165003
type: SequenceAnnotation
persistent identity: annotation1997226
display id: annotation1997226
version: 1
top level: BBa_K165090
location: https://synbiohub.org/public/igem/BBa_K165090/annotation1997226/range1997226/1
component: BBa_K165003

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type: Range
persistent identity: range1997226
display id: range1997226
version: 1
direction: direction/reverse
top level: BBa_K165090
start: 839
end: 3649
orientation: inline

BBa_K165004
type: SequenceAnnotation
persistent identity: annotation1997228
display id: annotation1997228
version: 1
top level: BBa_K165090
location: https://synbiohub.org/public/igem/BBa_K165090/annotation1997228/range1997228/1
component: BBa_K165004

https://synbiohub.org/public/igem/BBa_K165090/annotation1997228/range1997228/1
type: Range
persistent identity: range1997228
display id: range1997228
version: 1
direction: direction/reverse
top level: BBa_K165090
start: 4401
end: 5135
orientation: inline

BBa_K081020
type: ComponentDefinition
persistent identity: BBa K081020
display id: BBa_K081020
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: cI and lasR protein generator (TERM-)
creator: Lorenzo Pasotti, Paolo Magni
created: 2008-10-18T11:00:00Z
modified: 2015-05-08T01:08:35Z
discontinued: false
dominant: false
experience: experience/None
group u list: _227_
m user id: 0
owner id: 2583
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: This is a two-protein generator (cI and lasR) without transcriptional terminator. Strong RBS (efficiency=0.6).
mutable notes: We used BioBrick Standard Assembly.
mutable provenance: RBS: cI: lasR:
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_C0079
component: BBa_C0051
component: BBa_B0030
sequence annotation: BBa_C0051
sequence annotation: BBa_B0030
sequence annotation: BBa_C0079
sequence: https://synbiohub.org/public/igem/BBa_K081020_sequence/1

BBa_C0079
type: Component
persistent identity: component2249320
display id: component2249320
version: 1
top level: BBa_K081020
access: public
definition: lasr

BBa_C0051
type: Component
persistent identity: component2249312
display id: component2249312
version: 1
top level: BBa_K081020
access: public
definition: cI lam

BBa_B0030
type: Component
persistent identity: component2249314
display id: component2249314
version: 1
top level: BBa_K081020
access: public
definition: https://synbiohub.org/public/igem/BBa_B0030/1

BBa_B0030
type: Component
persistent identity: component2249307
display id: component2249307
version: 1
top level: BBa_K081020
access: public
definition: https://synbiohub.org/public/igem/BBa_B0030/1

BBa_C0051
type: SequenceAnnotation
persistent identity: annotation2249312
display id: annotation2249312
version: 1
top level: BBa_K081020
location: https://synbiohub.org/public/igem/BBa_K081020/annotation2249312/range2249312/1
component: BBa_C0051

https://synbiohub.org/public/igem/BBa_K081020/annotation2249312/range2249312/1
type: Range
persistent identity: range2249312
display id: range2249312
version: 1
direction: direction/reverse
top level: BBa_K081020
start: 22
end: 796
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation2249314
display id: annotation2249314
version: 1
top level: BBa_K081020
location: https://synbiohub.org/public/igem/BBa_K081020/annotation2249314/range2249314/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_K081020/annotation2249314/range2249314/1
type: Range
persistent identity: range2249314
display id: range2249314
version: 1
direction: direction/forward
top level: BBa_K081020
start: 805
end: 819
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation2249307
display id: annotation2249307
version: 1
top level: BBa_K081020
location: https://synbiohub.org/public/igem/BBa_K081020/annotation2249307/range2249307/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_K081020/annotation2249307/range2249307/1
type: Range
persistent identity: range2249307
display id: range2249307
version: 1
direction: direction/forward
top level: BBa_K081020
start: 1
end: 15
orientation: inline

BBa_C0079
type: SequenceAnnotation
persistent identity: annotation2249320
display id: annotation2249320
version: 1
top level: BBa_K081020
location: https://synbiohub.org/public/igem/BBa_K081020/annotation2249320/range2249320/1
component: BBa_C0079

https://synbiohub.org/public/igem/BBa_K081020/annotation2249320/range2249320/1
type: Range
persistent identity: range2249320
display id: range2249320
version: 1
direction: direction/reverse
top level: BBa_K081020
start: 826
end: 1606
orientation: inline

BBa_K404106
type: ComponentDefinition
persistent identity: BBa K404106
display id: BBa_K404106
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: phTERT promoter
creator: Freiburg Bioware 2010
created: 2010-08-27T11:00:00Z
modified: 2015-05-08T01:12:23Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 7049
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: phTERT promoter
sequence: https://synbiohub.org/public/igem/BBa_K404106_sequence/1

phTERT promoter
type: SequenceAnnotation
persistent identity: annotation2093171
display id: annotation2093171
version: 1
top level: BBa_K404106
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K404106/annotation2093171/range2093171/1

https://synbiohub.org/public/igem/BBa_K404106/annotation2093171/range2093171/1
type: Range
persistent identity: range2093171
display id: range2093171
version: 1
direction: direction/forward
top level: BBa_K404106
start: 1
end: 459
orientation: inline

BBa_K545005
type: ComponentDefinition
persistent identity: BBa K545005
display id: BBa_K545005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: fha
creator: Grenoble 2011
created: 2011-09-19T11:00:00Z
modified: 2015-05-08T01:12:39Z
discontinued: false
dominant: false
experience: experience/None
group u list: _712_
m user id: 0
owner id: 8648
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: Fha is a RBS containing a binding site for the rsmA protein. Fixing this RBS, rsmA inhibits the transcription, which is recovered thanks to the rsmY iRNA, when it binds to the rsmA repressor.
mutable notes: This part was build following the standard construction protocol.
mutable provenance: This RBS come from Pseudomonas aeruginosa.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: rbs
sequence: https://synbiohub.org/public/igem/BBa_K545005_sequence/1

rbs
type: SequenceAnnotation
persistent identity: annotation2137191
display id: annotation2137191
version: 1
top level: BBa_K545005
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K545005/annotation2137191/range2137191/1

https://synbiohub.org/public/igem/BBa_K545005/annotation2137191/range2137191/1
type: Range
persistent identity: range2137191
display id: range2137191
version: 1
direction: direction/forward
top level: BBa_K545005
start: 1
end: 137
orientation: inline

BBa_K1520013
type: ComponentDefinition
persistent identity: BBa K1520013
display id: BBa_K1520013
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Prlux-rbs-rfp-rbs-luxI-rbs-luxR-Ter
creator: yue wu
created: 2014-09-21T11:00:00Z
modified: 2015-05-08T01:10:49Z
discontinued: false
dominant: false
group u list: _1902_
m user id: 0
owner id: 21318
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Prlux-rbs-rfp-rbs-luxI-rbs-luxR-Ter. It produces rfp, luxI and luxR when there is enough luxR and AHL.
mutable notes: None
mutable provenance: Prlux, rfp, luxI, luxR, Ter
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1520500
component: BBa_K1520504
component: BBa_R0062
component: BBa_K1520029
component: BBa_K1520501
sequence annotation: BBa_K1520504
sequence annotation: BBa_K1520500
sequence annotation: BBa_K1520501
sequence annotation: BBa_R0062
sequence annotation: BBa_K1520029
sequence: https://synbiohub.org/public/igem/BBa_K1520013_sequence/1

BBa_K1520500
type: Component
persistent identity: component2384984
display id: component2384984
version: 1
top level: BBa_K1520013
access: public
definition: https://synbiohub.org/public/igem/BBa_K1520500/1

BBa_K1520504
type: Component
persistent identity: component2384987
display id: component2384987
version: 1
top level: BBa_K1520013
access: public
definition: https://synbiohub.org/public/igem/BBa_K1520504/1

BBa_R0062
type: Component
persistent identity: component2384979
display id: component2384979
version: 1
top level: BBa_K1520013
access: public
definition: https://synbiohub.org/public/igem/BBa_R0062/1

BBa_K1520029
type: Component
persistent identity: component2384991
display id: component2384991
version: 1
top level: BBa_K1520013
access: public
definition: https://synbiohub.org/public/igem/BBa_K1520029/1

BBa_K1520501
type: Component
persistent identity: component2384990
display id: component2384990
version: 1
top level: BBa_K1520013
access: public
definition: BBa_K1520501

BBa_K1520504
type: SequenceAnnotation
persistent identity: annotation2384987
display id: annotation2384987
version: 1
top level: BBa_K1520013
location: https://synbiohub.org/public/igem/BBa_K1520013/annotation2384987/range2384987/1
component: BBa_K1520504

https://synbiohub.org/public/igem/BBa_K1520013/annotation2384987/range2384987/1
type: Range
persistent identity: range2384987
display id: range2384987
version: 1
direction: direction/reverse
top level: BBa_K1520013
start: 804
end: 1464
orientation: inline

BBa_K1520500
type: SequenceAnnotation
persistent identity: annotation2384984
display id: annotation2384984
version: 1
top level: BBa_K1520013
location: https://synbiohub.org/public/igem/BBa_K1520013/annotation2384984/range2384984/1
component: BBa_K1520500

https://synbiohub.org/public/igem/BBa_K1520013/annotation2384984/range2384984/1
type: Range
persistent identity: range2384984
display id: range2384984
version: 1
direction: direction/reverse
top level: BBa_K1520013
start: 64
end: 795
orientation: inline

BBa_K1520501
type: SequenceAnnotation
persistent identity: annotation2384990
display id: annotation2384990
version: 1
top level: BBa_K1520013
location: https://synbiohub.org/public/igem/BBa_K1520013/annotation2384990/range2384990/1
component: BBa_K1520501

https://synbiohub.org/public/igem/BBa_K1520013/annotation2384990/range2384990/1
type: Range
persistent identity: range2384990
display id: range2384990
version: 1
direction: direction/reverse
top level: BBa_K1520013
start: 1473
end: 2271
orientation: inline

BBa_R0062
type: SequenceAnnotation
persistent identity: annotation2384979
display id: annotation2384979
version: 1
top level: BBa_K1520013
location: https://synbiohub.org/public/igem/BBa_K1520013/annotation2384979/range2384979/1
component: BBa_R0062

https://synbiohub.org/public/igem/BBa_K1520013/annotation2384979/range2384979/1
type: Range
persistent identity: range2384979
display id: range2384979
version: 1
direction: direction/forward
top level: BBa_K1520013
start: 1
end: 55
orientation: inline

BBa_K1520029
type: SequenceAnnotation
persistent identity: annotation2384991
display id: annotation2384991
version: 1
top level: BBa_K1520013
location: https://synbiohub.org/public/igem/BBa_K1520013/annotation2384991/range2384991/1
component: BBa_K1520029

https://synbiohub.org/public/igem/BBa_K1520013/annotation2384991/range2384991/1
type: Range
persistent identity: range2384991
display id: range2384991
version: 1
direction: direction/reverse
top level: BBa_K1520013
start: 2280
end: 2408
orientation: inline

BBa_P0150
type: ComponentDefinition
persistent identity: BBa P0150
display id: BBa_P0150
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PoPS -> cI (HK022) [S0164]
creator: Randy Rettberg
created: 2004-04-24T11:00:00Z
modified: 2015-05-08T01:14:10Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Protein generator converting TIPS to the protein cI (HK022). Used as the input section for Quad Part Inverter Q01500
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0031
component: BBa_C0050
component: BBa_B0010
component: BBa_B0012
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_C0050
sequence annotation: BBa_B0031
sequence: https://synbiohub.org/public/igem/BBa_P0150_sequence/1

BBa_B0031
type: Component
persistent identity: component944464
display id: component944464
version: 1
top level: BBa_P0150
access: public
definition: https://synbiohub.org/public/igem/BBa_B0031/1

BBa_C0050
type: Component
persistent identity: component944479
display id: component944479
version: 1
top level: BBa_P0150
access: public
definition: cI HK022

BBa_B0010
type: Component
persistent identity: component944487
display id: component944487
version: 1
top level: BBa_P0150
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0012
type: Component
persistent identity: component944497
display id: component944497
version: 1
top level: BBa_P0150
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation944487
display id: annotation944487
version: 1
top level: BBa_P0150
location: https://synbiohub.org/public/igem/BBa_P0150/annotation944487/range944487/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_P0150/annotation944487/range944487/1
type: Range
persistent identity: range944487
display id: range944487
version: 1
direction: direction/forward
top level: BBa_P0150
start: 798
end: 877
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation944497
display id: annotation944497
version: 1
top level: BBa_P0150
location: https://synbiohub.org/public/igem/BBa_P0150/annotation944497/range944497/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_P0150/annotation944497/range944497/1
type: Range
persistent identity: range944497
display id: range944497
version: 1
direction: direction/forward
top level: BBa_P0150
start: 886
end: 926
orientation: inline

BBa_C0050
type: SequenceAnnotation
persistent identity: annotation944479
display id: annotation944479
version: 1
top level: BBa_P0150
location: https://synbiohub.org/public/igem/BBa_P0150/annotation944479/range944479/1
component: BBa_C0050

https://synbiohub.org/public/igem/BBa_P0150/annotation944479/range944479/1
type: Range
persistent identity: range944479
display id: range944479
version: 1
direction: direction/forward
top level: BBa_P0150
start: 21
end: 764
orientation: inline

BBa_B0031
type: SequenceAnnotation
persistent identity: annotation944464
display id: annotation944464
version: 1
top level: BBa_P0150
location: https://synbiohub.org/public/igem/BBa_P0150/annotation944464/range944464/1
component: BBa_B0031

https://synbiohub.org/public/igem/BBa_P0150/annotation944464/range944464/1
type: Range
persistent identity: range944464
display id: range944464
version: 1
direction: direction/forward
top level: BBa_P0150
start: 1
end: 14
orientation: inline

BBa_K1682002
type: ComponentDefinition
persistent identity: BBa K1682002
display id: BBa_K1682002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PkdpF[-15, T>A]
creator: iGEM15_HKUST-Rice
created: 2015-08-09T11:00:00Z
modified: 2015-09-18T09:15:41Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2100_
m user id: 8758
owner id: 21235
owning group id: 9
status: status/Planning
bookmark: false
mutable description: kdpFp[-15, T>A]
mutable notes: kdpFp[-15, T>A]
mutable provenance: kdpFp[-15, T>A]
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -35
sequence annotation: -10
sequence annotation: phospho-KdpE binding site
sequence annotation: EcoRI Illegal site removed T->A
sequence: https://synbiohub.org/public/igem/BBa_K1682002_sequence/1

-35
type: SequenceAnnotation
persistent identity: annotation2473587
display id: annotation2473587
version: 1
top level: BBa_K1682002
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1682002/annotation2473587/range2473587/1

https://synbiohub.org/public/igem/BBa_K1682002/annotation2473587/range2473587/1
type: Range
persistent identity: range2473587
display id: range2473587
version: 1
direction: direction/forward
top level: BBa_K1682002
start: 42
end: 47
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2473586
display id: annotation2473586
version: 1
top level: BBa_K1682002
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1682002/annotation2473586/range2473586/1

https://synbiohub.org/public/igem/BBa_K1682002/annotation2473586/range2473586/1
type: Range
persistent identity: range2473586
display id: range2473586
version: 1
direction: direction/forward
top level: BBa_K1682002
start: 69
end: 74
orientation: inline

phospho-KdpE binding site
type: SequenceAnnotation
persistent identity: annotation2473585
display id: annotation2473585
version: 1
top level: BBa_K1682002
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1682002/annotation2473585/range2473585/1

https://synbiohub.org/public/igem/BBa_K1682002/annotation2473585/range2473585/1
type: Range
persistent identity: range2473585
display id: range2473585
version: 1
direction: direction/forward
top level: BBa_K1682002
start: 8
end: 24
orientation: inline

EcoRI Illegal site removed T->A
type: SequenceAnnotation
persistent identity: annotation2475205
display id: annotation2475205
version: 1
top level: BBa_K1682002
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K1682002/annotation2475205/range2475205/1

https://synbiohub.org/public/igem/BBa_K1682002/annotation2475205/range2475205/1
type: Range
persistent identity: range2475205
display id: range2475205
version: 1
direction: direction/forward
top level: BBa_K1682002
start: 65
end: 66
orientation: inline

BBa_K1486013
type: ComponentDefinition
persistent identity: BBa K1486013
display id: BBa_K1486013
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CpxR-IFP[2] under pBAD
creator: iGem EPFL 2014
created: 2014-08-31T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part has a fused protein downstream of an arabinose-inducible promoter: CpxR-IFP[2]. Biobrick BBa_K1486012 has CpxR-IFP[1] with an arabinose promoter. This construct was made in both orientations: BBa_K1486015 is IFP[2]-CpxR (with an arabinose promoter).
mutable notes: Both orientations were made, as well as for IFP[1] so all dimerizations could be tested (see main page for biobrick numbers). Flexible linkers were used to fuse both proteins, and an RBS was put before each one of them.
mutable provenance: to edit
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K1486004
component: BBa_K1486007
component: BBa_K1486000
component: BBa_I0500
component: BBa_B0034
sequence annotation: BBa_K1486000
sequence annotation: BBa_B0034
sequence annotation: BBa_K1486004
sequence annotation: BBa_I0500
sequence annotation: BBa_K1486007
sequence: https://synbiohub.org/public/igem/BBa_K1486013_sequence/1

BBa_K1486004
type: Component
persistent identity: component2382304
display id: component2382304
version: 1
top level: BBa_K1486013
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486004/1

BBa_K1486007
type: Component
persistent identity: component2382308
display id: component2382308
version: 1
top level: BBa_K1486013
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486007/1

BBa_K1486000
type: Component
persistent identity: component2382303
display id: component2382303
version: 1
top level: BBa_K1486013
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486000/1

BBa_I0500
type: Component
persistent identity: component2382297
display id: component2382297
version: 1
top level: BBa_K1486013
access: public
definition: https://synbiohub.org/public/igem/BBa_I0500/1

BBa_B0034
type: Component
persistent identity: component2382299
display id: component2382299
version: 1
top level: BBa_K1486013
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K1486000
type: SequenceAnnotation
persistent identity: annotation2382303
display id: annotation2382303
version: 1
top level: BBa_K1486013
location: https://synbiohub.org/public/igem/BBa_K1486013/annotation2382303/range2382303/1
component: BBa_K1486000

https://synbiohub.org/public/igem/BBa_K1486013/annotation2382303/range2382303/1
type: Range
persistent identity: range2382303
display id: range2382303
version: 1
direction: direction/reverse
top level: BBa_K1486013
start: 1223
end: 1924
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2382299
display id: annotation2382299
version: 1
top level: BBa_K1486013
location: https://synbiohub.org/public/igem/BBa_K1486013/annotation2382299/range2382299/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1486013/annotation2382299/range2382299/1
type: Range
persistent identity: range2382299
display id: range2382299
version: 1
direction: direction/reverse
top level: BBa_K1486013
start: 1211
end: 1222
orientation: inline

BBa_K1486004
type: SequenceAnnotation
persistent identity: annotation2382304
display id: annotation2382304
version: 1
top level: BBa_K1486013
location: https://synbiohub.org/public/igem/BBa_K1486013/annotation2382304/range2382304/1
component: BBa_K1486004

https://synbiohub.org/public/igem/BBa_K1486013/annotation2382304/range2382304/1
type: Range
persistent identity: range2382304
display id: range2382304
version: 1
direction: direction/reverse
top level: BBa_K1486013
start: 1925
end: 1954
orientation: inline

BBa_I0500
type: SequenceAnnotation
persistent identity: annotation2382297
display id: annotation2382297
version: 1
top level: BBa_K1486013
location: https://synbiohub.org/public/igem/BBa_K1486013/annotation2382297/range2382297/1
component: BBa_I0500

https://synbiohub.org/public/igem/BBa_K1486013/annotation2382297/range2382297/1
type: Range
persistent identity: range2382297
display id: range2382297
version: 1
direction: direction/reverse
top level: BBa_K1486013
start: 1
end: 1210
orientation: inline

BBa_K1486007
type: SequenceAnnotation
persistent identity: annotation2382308
display id: annotation2382308
version: 1
top level: BBa_K1486013
location: https://synbiohub.org/public/igem/BBa_K1486013/annotation2382308/range2382308/1
component: BBa_K1486007

https://synbiohub.org/public/igem/BBa_K1486013/annotation2382308/range2382308/1
type: Range
persistent identity: range2382308
display id: range2382308
version: 1
direction: direction/reverse
top level: BBa_K1486013
start: 1955
end: 2533
orientation: inline

Rec-Open
type: ComponentDefinition
persistent identity: BBa I726081
display id: BBa_I726081
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Rec-LRY.RC
creator: Bangalore iGEM
created: 2007-07-03T11:00:00Z
modified: 2015-08-31T04:07:55Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _107_
m user id: 0
owner id: 2077
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: Abcdefghijkl...
mutable notes: none
mutable provenance: Registry
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_B0034
component: BBa_B0010
component: BBa_E0020
component: BBa_E0030
component: BBa_R0011
component: BBa_C0062
component: BBa_B0012
component: BBa_R0062
sequence annotation: BBa_E0030
sequence annotation: BBa_R0062
sequence annotation: BBa_C0062
sequence annotation: BBa_B0012
sequence annotation: BBa_B0034
sequence annotation: BBa_B0010
sequence annotation: BBa_E0020
sequence annotation: BBa_R0011
sequence: https://synbiohub.org/public/igem/BBa_I726081_sequence/1

BBa_B0034
type: Component
persistent identity: component2296189
display id: component2296189
version: 1
top level: Rec-Open
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0010
type: Component
persistent identity: component2296177
display id: component2296177
version: 1
top level: Rec-Open
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0034
type: Component
persistent identity: component2296175
display id: component2296175
version: 1
top level: Rec-Open
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_E0020
type: Component
persistent identity: component2296190
display id: component2296190
version: 1
top level: Rec-Open
access: public
definition: https://synbiohub.org/public/igem/BBa_E0020/1

BBa_E0030
type: Component
persistent identity: component2296176
display id: component2296176
version: 1
top level: Rec-Open
access: public
definition: https://synbiohub.org/public/igem/BBa_E0030/1

BBa_R0011
type: Component
persistent identity: component2296161
display id: component2296161
version: 1
top level: Rec-Open
access: public
definition: https://synbiohub.org/public/igem/BBa_R0011/1

BBa_C0062
type: Component
persistent identity: component2296170
display id: component2296170
version: 1
top level: Rec-Open
access: public
definition: luxr

BBa_B0012
type: Component
persistent identity: component2296193
display id: component2296193
version: 1
top level: Rec-Open
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0010
type: Component
persistent identity: component2296191
display id: component2296191
version: 1
top level: Rec-Open
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0012
type: Component
persistent identity: component2296179
display id: component2296179
version: 1
top level: Rec-Open
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_R0062
type: Component
persistent identity: component2296184
display id: component2296184
version: 1
top level: Rec-Open
access: public
definition: https://synbiohub.org/public/igem/BBa_R0062/1

BBa_B0034
type: Component
persistent identity: component2296167
display id: component2296167
version: 1
top level: Rec-Open
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_E0030
type: SequenceAnnotation
persistent identity: annotation2296176
display id: annotation2296176
version: 1
top level: Rec-Open
location: https://synbiohub.org/public/igem/BBa_I726081/annotation2296176/range2296176/1
component: BBa_E0030

https://synbiohub.org/public/igem/BBa_I726081/annotation2296176/range2296176/1
type: Range
persistent identity: range2296176
display id: range2296176
version: 1
direction: direction/reverse
top level: Rec-Open
start: 889
end: 1611
orientation: inline

BBa_R0062
type: SequenceAnnotation
persistent identity: annotation2296184
display id: annotation2296184
version: 1
top level: Rec-Open
location: https://synbiohub.org/public/igem/BBa_I726081/annotation2296184/range2296184/1
component: BBa_R0062

https://synbiohub.org/public/igem/BBa_I726081/annotation2296184/range2296184/1
type: Range
persistent identity: range2296184
display id: range2296184
version: 1
direction: direction/forward
top level: Rec-Open
start: 1757
end: 1811
orientation: inline

BBa_C0062
type: SequenceAnnotation
persistent identity: annotation2296170
display id: annotation2296170
version: 1
top level: Rec-Open
location: https://synbiohub.org/public/igem/BBa_I726081/annotation2296170/range2296170/1
component: BBa_C0062

https://synbiohub.org/public/igem/BBa_I726081/annotation2296170/range2296170/1
type: Range
persistent identity: range2296170
display id: range2296170
version: 1
direction: direction/forward
top level: Rec-Open
start: 82
end: 837
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2296193
display id: annotation2296193
version: 1
top level: Rec-Open
location: https://synbiohub.org/public/igem/BBa_I726081/annotation2296193/range2296193/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I726081/annotation2296193/range2296193/1
type: Range
persistent identity: range2296193
display id: range2296193
version: 1
direction: direction/forward
top level: Rec-Open
start: 2657
end: 2697
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2296189
display id: annotation2296189
version: 1
top level: Rec-Open
location: https://synbiohub.org/public/igem/BBa_I726081/annotation2296189/range2296189/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I726081/annotation2296189/range2296189/1
type: Range
persistent identity: range2296189
display id: range2296189
version: 1
direction: direction/reverse
top level: Rec-Open
start: 1820
end: 1831
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2296175
display id: annotation2296175
version: 1
top level: Rec-Open
location: https://synbiohub.org/public/igem/BBa_I726081/annotation2296175/range2296175/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I726081/annotation2296175/range2296175/1
type: Range
persistent identity: range2296175
display id: range2296175
version: 1
direction: direction/reverse
top level: Rec-Open
start: 871
end: 882
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2296177
display id: annotation2296177
version: 1
top level: Rec-Open
location: https://synbiohub.org/public/igem/BBa_I726081/annotation2296177/range2296177/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I726081/annotation2296177/range2296177/1
type: Range
persistent identity: range2296177
display id: range2296177
version: 1
direction: direction/forward
top level: Rec-Open
start: 1620
end: 1699
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2296167
display id: annotation2296167
version: 1
top level: Rec-Open
location: https://synbiohub.org/public/igem/BBa_I726081/annotation2296167/range2296167/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I726081/annotation2296167/range2296167/1
type: Range
persistent identity: range2296167
display id: range2296167
version: 1
direction: direction/reverse
top level: Rec-Open
start: 64
end: 75
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2296179
display id: annotation2296179
version: 1
top level: Rec-Open
location: https://synbiohub.org/public/igem/BBa_I726081/annotation2296179/range2296179/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I726081/annotation2296179/range2296179/1
type: Range
persistent identity: range2296179
display id: range2296179
version: 1
direction: direction/forward
top level: Rec-Open
start: 1708
end: 1748
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2296191
display id: annotation2296191
version: 1
top level: Rec-Open
location: https://synbiohub.org/public/igem/BBa_I726081/annotation2296191/range2296191/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I726081/annotation2296191/range2296191/1
type: Range
persistent identity: range2296191
display id: range2296191
version: 1
direction: direction/forward
top level: Rec-Open
start: 2569
end: 2648
orientation: inline

BBa_E0020
type: SequenceAnnotation
persistent identity: annotation2296190
display id: annotation2296190
version: 1
top level: Rec-Open
location: https://synbiohub.org/public/igem/BBa_I726081/annotation2296190/range2296190/1
component: BBa_E0020

https://synbiohub.org/public/igem/BBa_I726081/annotation2296190/range2296190/1
type: Range
persistent identity: range2296190
display id: range2296190
version: 1
direction: direction/reverse
top level: Rec-Open
start: 1838
end: 2560
orientation: inline

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation2296161
display id: annotation2296161
version: 1
top level: Rec-Open
location: https://synbiohub.org/public/igem/BBa_I726081/annotation2296161/range2296161/1
component: BBa_R0011

https://synbiohub.org/public/igem/BBa_I726081/annotation2296161/range2296161/1
type: Range
persistent identity: range2296161
display id: range2296161
version: 1
direction: direction/forward
top level: Rec-Open
start: 1
end: 54
orientation: inline

BBa_K1486047
type: ComponentDefinition
persistent identity: BBa K1486047
display id: BBa_K1486047
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CpxR promoter RV
creator: EPFL iGem team 2014
created: 2014-10-06T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: to edit
mutable notes: to edit
mutable provenance: to edit
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K1486047_sequence/1

BBa_K1031111
type: ComponentDefinition
persistent identity: BBa K1031111
display id: BBa_K1031111
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: J23109-CapR-Terminator
creator: He Shuaixin
created: 2013-09-08T11:00:00Z
modified: 2015-05-08T01:08:47Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1338_
m user id: 0
owner id: 15843
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: 109-CapR-TT
mutable notes: molecular cloning
mutable provenance: synthesis Pseudomonas putida
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: CapR
sequence annotation: J23109
sequence annotation: B0015
sequence: https://synbiohub.org/public/igem/BBa_K1031111_sequence/1

CapR
type: SequenceAnnotation
persistent identity: annotation2361310
display id: annotation2361310
version: 1
top level: BBa_K1031111
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1031111/annotation2361310/range2361310/1

https://synbiohub.org/public/igem/BBa_K1031111/annotation2361310/range2361310/1
type: Range
persistent identity: range2361310
display id: range2361310
version: 1
direction: direction/forward
top level: BBa_K1031111
start: 62
end: 1753
orientation: inline

J23109
type: SequenceAnnotation
persistent identity: annotation2361309
display id: annotation2361309
version: 1
top level: BBa_K1031111
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1031111/annotation2361309/range2361309/1

https://synbiohub.org/public/igem/BBa_K1031111/annotation2361309/range2361309/1
type: Range
persistent identity: range2361309
display id: range2361309
version: 1
direction: direction/forward
top level: BBa_K1031111
start: 1
end: 35
orientation: inline

B0015
type: SequenceAnnotation
persistent identity: annotation2361311
display id: annotation2361311
version: 1
top level: BBa_K1031111
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1031111/annotation2361311/range2361311/1

https://synbiohub.org/public/igem/BBa_K1031111/annotation2361311/range2361311/1
type: Range
persistent identity: range2361311
display id: range2361311
version: 1
direction: direction/forward
top level: BBa_K1031111
start: 1760
end: 1888
orientation: inline

BBa_K404108
type: ComponentDefinition
persistent identity: BBa K404108
display id: BBa_K404108
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: hGH terminator
creator: Freiburg Bioware 2010
created: 2010-08-27T11:00:00Z
modified: 2015-05-08T01:12:23Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 7049
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: hGH terminator
sequence: https://synbiohub.org/public/igem/BBa_K404108_sequence/1

hGH terminator
type: SequenceAnnotation
persistent identity: annotation2093173
display id: annotation2093173
version: 1
top level: BBa_K404108
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404108/annotation2093173/range2093173/1

https://synbiohub.org/public/igem/BBa_K404108/annotation2093173/range2093173/1
type: Range
persistent identity: range2093173
display id: range2093173
version: 1
direction: direction/forward
top level: BBa_K404108
start: 1
end: 481
orientation: inline

GAL-LexA
type: ComponentDefinition
persistent identity: BBa K165068
display id: BBa_K165068
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pGAL1 + LexA activator on pRS305
creator: John Szymanski
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:10:56Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _267_
m user id: 0
owner id: 2512
owning group id: 58
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: -
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Device
role: SO:0000804
sequence: https://synbiohub.org/public/igem/BBa_K165068_sequence/1

BBa_K921000
type: ComponentDefinition
persistent identity: BBa K921000
display id: BBa_K921000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: T7 RNAP + IPTG->PoPs (Mutant I)
creator: Eric Pederson
created: 2012-09-30T11:00:00Z
modified: 2015-05-08T01:13:46Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1186_
m user id: 0
owner id: 12713
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: This is a mutant T7 promoter that includes a lacO sequence. This is repressible by the LacI protein and is induced in the presence of IPTG. The promoter sits in the 5' region of the gene of interest and initiates transcription when the cell expresses T7 RNA polymerase. Be sure you are using a cell strain that includes this gene (DE3).
mutable notes: There are three portions to the T7 promoter: the recognition site, the melting box (or TATA box) and the initation site. Mutations in these sequences caused differential behavior from the wild type.
mutable provenance: The part consists of a sequences that resemble a T7 promoter and the lacO sequence found in E. coli.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/T7
role: SO:0001207
sequence annotation: LacO
sequence annotation: Initiation
sequence annotation: T7 promoter
sequence: https://synbiohub.org/public/igem/BBa_K921000_sequence/1

LacO
type: SequenceAnnotation
persistent identity: annotation2205290
display id: annotation2205290
version: 1
top level: BBa_K921000
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K921000/annotation2205290/range2205290/1

https://synbiohub.org/public/igem/BBa_K921000/annotation2205290/range2205290/1
type: Range
persistent identity: range2205290
display id: range2205290
version: 1
direction: direction/forward
top level: BBa_K921000
start: 19
end: 46
orientation: inline

Initiation
type: SequenceAnnotation
persistent identity: annotation2205291
display id: annotation2205291
version: 1
top level: BBa_K921000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K921000/annotation2205291/range2205291/1

https://synbiohub.org/public/igem/BBa_K921000/annotation2205291/range2205291/1
type: Range
persistent identity: range2205291
display id: range2205291
version: 1
direction: direction/forward
top level: BBa_K921000
start: 19
end: 20
orientation: inline

T7 promoter
type: SequenceAnnotation
persistent identity: annotation2205289
display id: annotation2205289
version: 1
top level: BBa_K921000
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K921000/annotation2205289/range2205289/1

https://synbiohub.org/public/igem/BBa_K921000/annotation2205289/range2205289/1
type: Range
persistent identity: range2205289
display id: range2205289
version: 1
direction: direction/forward
top level: BBa_K921000
start: 1
end: 19
orientation: inline

BBa_K404103
type: ComponentDefinition
persistent identity: BBa K404103
display id: BBa_K404103
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: p5 promoter
creator: Freiburg Bioware 2010
created: 2010-08-27T11:00:00Z
modified: 2015-05-08T01:12:23Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 7049
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: p5 promoter
sequence: https://synbiohub.org/public/igem/BBa_K404103_sequence/1

p5 promoter
type: SequenceAnnotation
persistent identity: annotation2093169
display id: annotation2093169
version: 1
top level: BBa_K404103
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K404103/annotation2093169/range2093169/1

https://synbiohub.org/public/igem/BBa_K404103/annotation2093169/range2093169/1
type: Range
persistent identity: range2093169
display id: range2093169
version: 1
direction: direction/forward
top level: BBa_K404103
start: 1
end: 166
orientation: inline

BBa_J06651
type: ComponentDefinition
persistent identity: BBa J06651
display id: BBa_J06651
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Test of BBa_R0052
creator: ytwang
created: 2005-06-13T11:00:00Z
modified: 2015-08-31T04:08:18Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _20_
m user id: 0
owner id: 340
owning group id: 20
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_R0052
component: BBa_E0030
component: BBa_B0010
component: BBa_B0012
component: BBa_B0034
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_E0030
sequence annotation: BBa_R0052
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_J06651_sequence/1

BBa_R0052
type: Component
persistent identity: component1533927
display id: component1533927
version: 1
top level: BBa_J06651
access: public
definition: https://synbiohub.org/public/igem/BBa_R0052/1

BBa_E0030
type: Component
persistent identity: component1533939
display id: component1533939
version: 1
top level: BBa_J06651
access: public
definition: https://synbiohub.org/public/igem/BBa_E0030/1

BBa_B0010
type: Component
persistent identity: component1533944
display id: component1533944
version: 1
top level: BBa_J06651
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0012
type: Component
persistent identity: component1533954
display id: component1533954
version: 1
top level: BBa_J06651
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0034
type: Component
persistent identity: component1533937
display id: component1533937
version: 1
top level: BBa_J06651
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1533944
display id: annotation1533944
version: 1
top level: BBa_J06651
location: https://synbiohub.org/public/igem/BBa_J06651/annotation1533944/range1533944/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_J06651/annotation1533944/range1533944/1
type: Range
persistent identity: range1533944
display id: range1533944
version: 1
direction: direction/forward
top level: BBa_J06651
start: 804
end: 883
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1533954
display id: annotation1533954
version: 1
top level: BBa_J06651
location: https://synbiohub.org/public/igem/BBa_J06651/annotation1533954/range1533954/1
component: BBa_B0012

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type: Range
persistent identity: range1533954
display id: range1533954
version: 1
direction: direction/forward
top level: BBa_J06651
start: 892
end: 932
orientation: inline

BBa_E0030
type: SequenceAnnotation
persistent identity: annotation1533939
display id: annotation1533939
version: 1
top level: BBa_J06651
location: https://synbiohub.org/public/igem/BBa_J06651/annotation1533939/range1533939/1
component: BBa_E0030

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type: Range
persistent identity: range1533939
display id: range1533939
version: 1
direction: direction/forward
top level: BBa_J06651
start: 73
end: 795
orientation: inline

BBa_R0052
type: SequenceAnnotation
persistent identity: annotation1533927
display id: annotation1533927
version: 1
top level: BBa_J06651
location: https://synbiohub.org/public/igem/BBa_J06651/annotation1533927/range1533927/1
component: BBa_R0052

https://synbiohub.org/public/igem/BBa_J06651/annotation1533927/range1533927/1
type: Range
persistent identity: range1533927
display id: range1533927
version: 1
direction: direction/forward
top level: BBa_J06651
start: 1
end: 46
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1533937
display id: annotation1533937
version: 1
top level: BBa_J06651
location: https://synbiohub.org/public/igem/BBa_J06651/annotation1533937/range1533937/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_J06651/annotation1533937/range1533937/1
type: Range
persistent identity: range1533937
display id: range1533937
version: 1
direction: direction/forward
top level: BBa_J06651
start: 55
end: 66
orientation: inline

BBa_K1486009
type: ComponentDefinition
persistent identity: BBa K1486009
display id: BBa_K1486009
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CxpR & Split IFP1.4 [Nterm + Nterm]
creator: iGem EPFL 2014
created: 2014-08-26T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part has two fused proteins downstream of an arabinose-inducible promoter: CpxR-IFP[1] and CpxR-IFP[2]. This construct was made in all orientations: - CpxR-IFP[1] + CpxR-IFP[2] (BBa_K1286008) - IFP[1]-CpxR + CpxR-IFP[2] (BBa_K1486010) - CpxR-IFP[1] + IFP[2]-CpxR (BBa_K1486011)
mutable notes: All orientations were made, so all dimerizations could be tested (see main page for biobrick numbers). Flexible linkers were used for each of the fused proteins, and an RBS was put before each one of them.
mutable provenance: From Michnick Article. To Edit
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K1486007
component: BBa_I0500
component: BBa_K1486004
component: BBa_K1486000
component: BBa_K1486006
component: BBa_K1486052
component: BBa_B0034
sequence annotation: BBa_B0034
sequence annotation: BBa_K1486007
sequence annotation: BBa_K1486004
sequence annotation: BBa_K1486000
sequence annotation: BBa_I0500
sequence annotation: BBa_K1486006
sequence annotation: BBa_K1486052
sequence: https://synbiohub.org/public/igem/BBa_K1486009_sequence/1

BBa_K1486007
type: Component
persistent identity: component2420783
display id: component2420783
version: 1
top level: BBa_K1486009
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486007/1

BBa_I0500
type: Component
persistent identity: component2420763
display id: component2420763
version: 1
top level: BBa_K1486009
access: public
definition: https://synbiohub.org/public/igem/BBa_I0500/1

BBa_K1486004
type: Component
persistent identity: component2420784
display id: component2420784
version: 1
top level: BBa_K1486009
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486004/1

BBa_K1486004
type: Component
persistent identity: component2420772
display id: component2420772
version: 1
top level: BBa_K1486009
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486004/1

BBa_K1486000
type: Component
persistent identity: component2420766
display id: component2420766
version: 1
top level: BBa_K1486009
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486000/1

BBa_K1486004
type: Component
persistent identity: component2420758
display id: component2420758
version: 1
top level: BBa_K1486009
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486004/1

BBa_K1486006
type: Component
persistent identity: component2420771
display id: component2420771
version: 1
top level: BBa_K1486009
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486006/1

BBa_K1486052
type: Component
persistent identity: component2420777
display id: component2420777
version: 1
top level: BBa_K1486009
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486052/1

BBa_B0034
type: Component
persistent identity: component2420779
display id: component2420779
version: 1
top level: BBa_K1486009
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0034
type: Component
persistent identity: component2420767
display id: component2420767
version: 1
top level: BBa_K1486009
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K1486000
type: Component
persistent identity: component2420776
display id: component2420776
version: 1
top level: BBa_K1486009
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486000/1

BBa_K1486000
type: Component
persistent identity: component2420788
display id: component2420788
version: 1
top level: BBa_K1486009
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486000/1

BBa_K1486007
type: Component
persistent identity: component2420756
display id: component2420756
version: 1
top level: BBa_K1486009
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486007/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2420779
display id: annotation2420779
version: 1
top level: BBa_K1486009
location: https://synbiohub.org/public/igem/BBa_K1486009/annotation2420779/range2420779/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1486009/annotation2420779/range2420779/1
type: Range
persistent identity: range2420779
display id: range2420779
version: 1
direction: direction/reverse
top level: BBa_K1486009
start: 2398
end: 2409
orientation: inline

BBa_K1486007
type: SequenceAnnotation
persistent identity: annotation2420783
display id: annotation2420783
version: 1
top level: BBa_K1486009
location: https://synbiohub.org/public/igem/BBa_K1486009/annotation2420783/range2420783/1
component: BBa_K1486007

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type: Range
persistent identity: range2420783
display id: range2420783
version: 1
direction: direction/reverse
top level: BBa_K1486009
start: 2410
end: 2994
orientation: inline

BBa_K1486004
type: SequenceAnnotation
persistent identity: annotation2420758
display id: annotation2420758
version: 1
top level: BBa_K1486009
location: https://synbiohub.org/public/igem/BBa_K1486009/annotation2420758/range2420758/1
component: BBa_K1486004

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type: Range
persistent identity: range2420758
display id: range2420758
version: 1
direction: direction/reverse
top level: BBa_K1486009
start: 2995
end: 3024
orientation: inline

BBa_K1486007
type: SequenceAnnotation
persistent identity: annotation2420756
display id: annotation2420756
version: 1
top level: BBa_K1486009
location: https://synbiohub.org/public/igem/BBa_K1486009/annotation2420756/range2420756/1
component: BBa_K1486007

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type: Range
persistent identity: range2420756
display id: range2420756
version: 1
direction: direction/reverse
top level: BBa_K1486009
start: 2410
end: 2994
orientation: inline

BBa_K1486000
type: SequenceAnnotation
persistent identity: annotation2420766
display id: annotation2420766
version: 1
top level: BBa_K1486009
location: https://synbiohub.org/public/igem/BBa_K1486009/annotation2420766/range2420766/1
component: BBa_K1486000

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type: Range
persistent identity: range2420766
display id: range2420766
version: 1
direction: direction/reverse
top level: BBa_K1486009
start: 3025
end: 3726
orientation: inline

BBa_I0500
type: SequenceAnnotation
persistent identity: annotation2420763
display id: annotation2420763
version: 1
top level: BBa_K1486009
location: https://synbiohub.org/public/igem/BBa_K1486009/annotation2420763/range2420763/1
component: BBa_I0500

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type: Range
persistent identity: range2420763
display id: range2420763
version: 1
direction: direction/reverse
top level: BBa_K1486009
start: 1
end: 1210
orientation: inline

BBa_K1486004
type: SequenceAnnotation
persistent identity: annotation2420772
display id: annotation2420772
version: 1
top level: BBa_K1486009
location: https://synbiohub.org/public/igem/BBa_K1486009/annotation2420772/range2420772/1
component: BBa_K1486004

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type: Range
persistent identity: range2420772
display id: range2420772
version: 1
direction: direction/reverse
top level: BBa_K1486009
start: 1628
end: 1657
orientation: inline

BBa_K1486000
type: SequenceAnnotation
persistent identity: annotation2420788
display id: annotation2420788
version: 1
top level: BBa_K1486009
location: https://synbiohub.org/public/igem/BBa_K1486009/annotation2420788/range2420788/1
component: BBa_K1486000

https://synbiohub.org/public/igem/BBa_K1486009/annotation2420788/range2420788/1
type: Range
persistent identity: range2420788
display id: range2420788
version: 1
direction: direction/reverse
top level: BBa_K1486009
start: 3025
end: 3726
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2420767
display id: annotation2420767
version: 1
top level: BBa_K1486009
location: https://synbiohub.org/public/igem/BBa_K1486009/annotation2420767/range2420767/1
component: BBa_B0034

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type: Range
persistent identity: range2420767
display id: range2420767
version: 1
direction: direction/reverse
top level: BBa_K1486009
start: 1211
end: 1222
orientation: inline

BBa_K1486000
type: SequenceAnnotation
persistent identity: annotation2420776
display id: annotation2420776
version: 1
top level: BBa_K1486009
location: https://synbiohub.org/public/igem/BBa_K1486009/annotation2420776/range2420776/1
component: BBa_K1486000

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type: Range
persistent identity: range2420776
display id: range2420776
version: 1
direction: direction/reverse
top level: BBa_K1486009
start: 1658
end: 2359
orientation: inline

BBa_K1486004
type: SequenceAnnotation
persistent identity: annotation2420784
display id: annotation2420784
version: 1
top level: BBa_K1486009
location: https://synbiohub.org/public/igem/BBa_K1486009/annotation2420784/range2420784/1
component: BBa_K1486004

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type: Range
persistent identity: range2420784
display id: range2420784
version: 1
direction: direction/reverse
top level: BBa_K1486009
start: 2995
end: 3024
orientation: inline

BBa_K1486006
type: SequenceAnnotation
persistent identity: annotation2420771
display id: annotation2420771
version: 1
top level: BBa_K1486009
location: https://synbiohub.org/public/igem/BBa_K1486009/annotation2420771/range2420771/1
component: BBa_K1486006

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type: Range
persistent identity: range2420771
display id: range2420771
version: 1
direction: direction/reverse
top level: BBa_K1486009
start: 1223
end: 1627
orientation: inline

BBa_K1486052
type: SequenceAnnotation
persistent identity: annotation2420777
display id: annotation2420777
version: 1
top level: BBa_K1486009
location: https://synbiohub.org/public/igem/BBa_K1486009/annotation2420777/range2420777/1
component: BBa_K1486052

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type: Range
persistent identity: range2420777
display id: range2420777
version: 1
direction: direction/reverse
top level: BBa_K1486009
start: 2360
end: 2397
orientation: inline

BBa_P0312
type: ComponentDefinition
persistent identity: BBa P0312
display id: BBa_P0312
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PoPS -> LacI [S0156]
creator: Randy Rettberg
created: 2004-04-26T11:00:00Z
modified: 2015-05-08T01:14:10Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0010
component: BBa_B0012
component: BBa_C0012
component: BBa_B0033
sequence annotation: BBa_B0012
sequence annotation: BBa_B0010
sequence annotation: BBa_C0012
sequence annotation: BBa_B0033
sequence: https://synbiohub.org/public/igem/BBa_P0312_sequence/1

BBa_B0010
type: Component
persistent identity: component944624
display id: component944624
version: 1
top level: BBa_P0312
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0012
type: Component
persistent identity: component944634
display id: component944634
version: 1
top level: BBa_P0312
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_C0012
type: Component
persistent identity: component944617
display id: component944617
version: 1
top level: BBa_P0312
access: public
definition: lacI

BBa_B0033
type: Component
persistent identity: component944606
display id: component944606
version: 1
top level: BBa_P0312
access: public
definition: https://synbiohub.org/public/igem/BBa_B0033/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation944634
display id: annotation944634
version: 1
top level: BBa_P0312
location: https://synbiohub.org/public/igem/BBa_P0312/annotation944634/range944634/1
component: BBa_B0012

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type: Range
persistent identity: range944634
display id: range944634
version: 1
direction: direction/forward
top level: BBa_P0312
start: 1267
end: 1307
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation944624
display id: annotation944624
version: 1
top level: BBa_P0312
location: https://synbiohub.org/public/igem/BBa_P0312/annotation944624/range944624/1
component: BBa_B0010

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type: Range
persistent identity: range944624
display id: range944624
version: 1
direction: direction/forward
top level: BBa_P0312
start: 1179
end: 1258
orientation: inline

BBa_C0012
type: SequenceAnnotation
persistent identity: annotation944617
display id: annotation944617
version: 1
top level: BBa_P0312
location: https://synbiohub.org/public/igem/BBa_P0312/annotation944617/range944617/1
component: BBa_C0012

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type: Range
persistent identity: range944617
display id: range944617
version: 1
direction: direction/forward
top level: BBa_P0312
start: 18
end: 1145
orientation: inline

BBa_B0033
type: SequenceAnnotation
persistent identity: annotation944606
display id: annotation944606
version: 1
top level: BBa_P0312
location: https://synbiohub.org/public/igem/BBa_P0312/annotation944606/range944606/1
component: BBa_B0033

https://synbiohub.org/public/igem/BBa_P0312/annotation944606/range944606/1
type: Range
persistent identity: range944606
display id: range944606
version: 1
direction: direction/forward
top level: BBa_P0312
start: 1
end: 11
orientation: inline

BBa_K1315000
type: ComponentDefinition
persistent identity: BBa K1315000
display id: BBa_K1315000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pqsR
creator: Dimitrios Michailidis
created: 2014-09-19T11:00:00Z
modified: 2015-05-08T01:09:48Z
discontinued: true
dominant: false
experience: experience/None
group u list: _1690_
m user id: 0
owner id: 21812
owning group id: 9
sample status: Discontinued
status: status/Deleted
bookmark: false
mutable description: The Pseydomonas quinolone signal (PQS) as well as its precursor 2-heptyl-4-quinolone (HHQ)are very important in mechanisms controlling virulence in Pseudomonas aeruginosa. P. aeruginosa has a key role in Cystic Fibrosis (CF). PQS can initiate signalling for various processes like virulence, motility, and biofilm formation. A good number of these processes are facilitated by PqsR, which is a transcriptional factor. An activated PqsR will go to the nucleus of the cells and activate the respective genes. http://www.ncbi.nlm.nih.gov/pubmed/21539583 http://www.ncbi.nlm.nih.gov/pubmed/?term=pqsr-dependent+and+pqsr-independent+regulation+of+motility+and+biofilm+formation+by+pqs http://www.ncbi.nlm.nih.gov/pubmed/12393198
mutable notes: The sequence did not have any restriction sites to remove so it was immediately PCRed out of the genome and had the registry prefix and suffix added to it. It was then ligated into the pSB1C3 vector.
mutable provenance: The source of this part is the genome of Pseudomonas aeruginosa PAO1 provided by the Robert Ryan lab in the Molecular Microbiology department of the University of Dundee, UK.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: PqsR
sequence: https://synbiohub.org/public/igem/BBa_K1315000_sequence/1

PqsR
type: SequenceAnnotation
persistent identity: annotation2384759
display id: annotation2384759
version: 1
top level: BBa_K1315000
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1315000/annotation2384759/range2384759/1

https://synbiohub.org/public/igem/BBa_K1315000/annotation2384759/range2384759/1
type: Range
persistent identity: range2384759
display id: range2384759
version: 1
direction: direction/forward
top level: BBa_K1315000
start: 1
end: 999
orientation: inline

BBa_K165022
type: ComponentDefinition
persistent identity: BBa K165022
display id: BBa_K165022
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Zif268-HIV synthetic activator (untagged)
creator: John Szymanski
created: 2008-10-27T12:00:00Z
modified: 2015-05-08T01:10:55Z
discontinued: false
dominant: false
group u list: _267_
m user id: 0
owner id: 2512
owning group id: 58
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This is a synthetic transcriptional activator for use in yeast. It contains the VP16 activation domain, the Zif268-HIV DNA-binding domain, the SV40 nuclear localization sequence, and the ADH1 terminator. It binds to the Zif268-HIV binding sites, activating transcription downstream.
mutable notes: -
mutable provenance: From David Drubin in the Silver Lab at Harvard.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence: https://synbiohub.org/public/igem/BBa_K165022_sequence/1

BBa_K1486010
type: ComponentDefinition
persistent identity: BBa K1486010
display id: BBa_K1486010
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CxpR & Split IFP1.4 [Nterm + Cterm]
creator: iGem EPFL 2014
created: 2014-08-26T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part has two fused proteins downstream of an arabinose-inducible promoter: CpxR-IFP[1] and CpxR-IFP[2]. This construct was made in all orientations: - CpxR-IFP[1] + CpxR-IFP[2] (BBa_K1286008) - IFP[1]-CpxR + CpxR-IFP[2] (BBa_K1486010) - CpxR-IFP[1] + IFP[2]-CpxR (BBa_K1486011)
mutable notes: All orientations were made, so all dimerizations could be tested (see main page for biobrick numbers). Flexible linkers were used for each of the fused proteins, and an RBS was put before each one of them.
mutable provenance: From Michnick Article. To Edit
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K1486007
component: BBa_I0500
component: BBa_B0034
component: BBa_K1486006
component: BBa_K1486000
component: BBa_K1486004
sequence annotation: BBa_K1486000
sequence annotation: BBa_K1486004
sequence annotation: BBa_B0034
sequence annotation: BBa_K1486007
sequence annotation: BBa_K1486006
sequence annotation: BBa_I0500
sequence: https://synbiohub.org/public/igem/BBa_K1486010_sequence/1

BBa_K1486007
type: Component
persistent identity: component2425006
display id: component2425006
version: 1
top level: BBa_K1486010
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486007/1

BBa_I0500
type: Component
persistent identity: component2424984
display id: component2424984
version: 1
top level: BBa_K1486010
access: public
definition: https://synbiohub.org/public/igem/BBa_I0500/1

BBa_B0034
type: Component
persistent identity: component2424997
display id: component2424997
version: 1
top level: BBa_K1486010
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K1486006
type: Component
persistent identity: component2424990
display id: component2424990
version: 1
top level: BBa_K1486010
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486006/1

BBa_K1486000
type: Component
persistent identity: component2424995
display id: component2424995
version: 1
top level: BBa_K1486010
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486000/1

BBa_B0034
type: Component
persistent identity: component2424986
display id: component2424986
version: 1
top level: BBa_K1486010
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K1486004
type: Component
persistent identity: component2425002
display id: component2425002
version: 1
top level: BBa_K1486010
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486004/1

BBa_K1486004
type: Component
persistent identity: component2424991
display id: component2424991
version: 1
top level: BBa_K1486010
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486004/1

BBa_K1486000
type: Component
persistent identity: component2425001
display id: component2425001
version: 1
top level: BBa_K1486010
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486000/1

BBa_K1486000
type: SequenceAnnotation
persistent identity: annotation2425001
display id: annotation2425001
version: 1
top level: BBa_K1486010
location: https://synbiohub.org/public/igem/BBa_K1486010/annotation2425001/range2425001/1
component: BBa_K1486000

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type: Range
persistent identity: range2425001
display id: range2425001
version: 1
direction: direction/reverse
top level: BBa_K1486010
start: 2372
end: 3073
orientation: inline

BBa_K1486004
type: SequenceAnnotation
persistent identity: annotation2425002
display id: annotation2425002
version: 1
top level: BBa_K1486010
location: https://synbiohub.org/public/igem/BBa_K1486010/annotation2425002/range2425002/1
component: BBa_K1486004

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type: Range
persistent identity: range2425002
display id: range2425002
version: 1
direction: direction/reverse
top level: BBa_K1486010
start: 3074
end: 3103
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2424997
display id: annotation2424997
version: 1
top level: BBa_K1486010
location: https://synbiohub.org/public/igem/BBa_K1486010/annotation2424997/range2424997/1
component: BBa_B0034

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type: Range
persistent identity: range2424997
display id: range2424997
version: 1
direction: direction/reverse
top level: BBa_K1486010
start: 2360
end: 2371
orientation: inline

BBa_K1486004
type: SequenceAnnotation
persistent identity: annotation2424991
display id: annotation2424991
version: 1
top level: BBa_K1486010
location: https://synbiohub.org/public/igem/BBa_K1486010/annotation2424991/range2424991/1
component: BBa_K1486004

https://synbiohub.org/public/igem/BBa_K1486010/annotation2424991/range2424991/1
type: Range
persistent identity: range2424991
display id: range2424991
version: 1
direction: direction/reverse
top level: BBa_K1486010
start: 1628
end: 1657
orientation: inline

BBa_K1486007
type: SequenceAnnotation
persistent identity: annotation2425006
display id: annotation2425006
version: 1
top level: BBa_K1486010
location: https://synbiohub.org/public/igem/BBa_K1486010/annotation2425006/range2425006/1
component: BBa_K1486007

https://synbiohub.org/public/igem/BBa_K1486010/annotation2425006/range2425006/1
type: Range
persistent identity: range2425006
display id: range2425006
version: 1
direction: direction/reverse
top level: BBa_K1486010
start: 3104
end: 3688
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2424986
display id: annotation2424986
version: 1
top level: BBa_K1486010
location: https://synbiohub.org/public/igem/BBa_K1486010/annotation2424986/range2424986/1
component: BBa_B0034

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type: Range
persistent identity: range2424986
display id: range2424986
version: 1
direction: direction/reverse
top level: BBa_K1486010
start: 1211
end: 1222
orientation: inline

BBa_K1486006
type: SequenceAnnotation
persistent identity: annotation2424990
display id: annotation2424990
version: 1
top level: BBa_K1486010
location: https://synbiohub.org/public/igem/BBa_K1486010/annotation2424990/range2424990/1
component: BBa_K1486006

https://synbiohub.org/public/igem/BBa_K1486010/annotation2424990/range2424990/1
type: Range
persistent identity: range2424990
display id: range2424990
version: 1
direction: direction/reverse
top level: BBa_K1486010
start: 1223
end: 1627
orientation: inline

BBa_I0500
type: SequenceAnnotation
persistent identity: annotation2424984
display id: annotation2424984
version: 1
top level: BBa_K1486010
location: https://synbiohub.org/public/igem/BBa_K1486010/annotation2424984/range2424984/1
component: BBa_I0500

https://synbiohub.org/public/igem/BBa_K1486010/annotation2424984/range2424984/1
type: Range
persistent identity: range2424984
display id: range2424984
version: 1
direction: direction/reverse
top level: BBa_K1486010
start: 1
end: 1210
orientation: inline

BBa_K1486000
type: SequenceAnnotation
persistent identity: annotation2424995
display id: annotation2424995
version: 1
top level: BBa_K1486010
location: https://synbiohub.org/public/igem/BBa_K1486010/annotation2424995/range2424995/1
component: BBa_K1486000

https://synbiohub.org/public/igem/BBa_K1486010/annotation2424995/range2424995/1
type: Range
persistent identity: range2424995
display id: range2424995
version: 1
direction: direction/reverse
top level: BBa_K1486010
start: 1658
end: 2359
orientation: inline

BBa_K404107
type: ComponentDefinition
persistent identity: BBa K404107
display id: BBa_K404107
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Beta-Globin-Intron
creator: Freiburg Bioware 2010
created: 2010-08-27T11:00:00Z
modified: 2015-05-08T01:12:23Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 7049
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: Beta-Globin-intron
sequence: https://synbiohub.org/public/igem/BBa_K404107_sequence/1

Beta-Globin-intron
type: SequenceAnnotation
persistent identity: annotation2093172
display id: annotation2093172
version: 1
top level: BBa_K404107
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404107/annotation2093172/range2093172/1

https://synbiohub.org/public/igem/BBa_K404107/annotation2093172/range2093172/1
type: Range
persistent identity: range2093172
display id: range2093172
version: 1
direction: direction/forward
top level: BBa_K404107
start: 1
end: 495
orientation: inline

lacI ts
type: ComponentDefinition
persistent identity: BBa J06501
display id: BBa_J06501
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LacI repressor (temperature-sensitive mut 265) (+LVA)
creator: ytwang
created: 2005-06-13T11:00:00Z
modified: 2015-08-31T04:08:18Z
discontinued: false
dominant: false
experience: experience/None
group u list: _20_
m user id: 0
owner id: 340
owning group id: 20
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Mutated from BBa_C0012 Repressor, LacI (LVA+) to be temperature sensitive. Mutation from Chao, et al., 2002: Gly265->Asp (GG(803)T->GAT)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: LVA
sequence annotation: TS mutation (G->A)
sequence annotation: barcode from LacI (BBa_C0012)
sequence annotation: LacI(ts)-LVA
sequence: https://synbiohub.org/public/igem/BBa_J06501_sequence/1

LVA
type: SequenceAnnotation
persistent identity: annotation1530907
display id: annotation1530907
version: 1
top level: lacI ts
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_J06501/annotation1530907/range1530907/1

https://synbiohub.org/public/igem/BBa_J06501/annotation1530907/range1530907/1
type: Range
persistent identity: range1530907
display id: range1530907
version: 1
direction: direction/forward
top level: lacI ts
start: 1090
end: 1128
orientation: inline

TS mutation (G->A)
type: SequenceAnnotation
persistent identity: annotation1530900
display id: annotation1530900
version: 1
top level: lacI ts
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_J06501/annotation1530900/range1530900/1

https://synbiohub.org/public/igem/BBa_J06501/annotation1530900/range1530900/1
type: Range
persistent identity: range1530900
display id: range1530900
version: 1
direction: direction/forward
top level: lacI ts
start: 803
end: 803
orientation: inline

barcode from LacI (BBa_C0012)
type: SequenceAnnotation
persistent identity: annotation1530914
display id: annotation1530914
version: 1
top level: lacI ts
role: SO:0000807
role: feature/barcode
location: https://synbiohub.org/public/igem/BBa_J06501/annotation1530914/range1530914/1

https://synbiohub.org/public/igem/BBa_J06501/annotation1530914/range1530914/1
type: Range
persistent identity: range1530914
display id: range1530914
version: 1
direction: direction/forward
top level: lacI ts
start: 1129
end: 1153
orientation: inline

LacI(ts)-LVA
type: SequenceAnnotation
persistent identity: annotation1530899
display id: annotation1530899
version: 1
top level: lacI ts
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J06501/annotation1530899/range1530899/1

https://synbiohub.org/public/igem/BBa_J06501/annotation1530899/range1530899/1
type: Range
persistent identity: range1530899
display id: range1530899
version: 1
direction: direction/forward
top level: lacI ts
start: 1
end: 1128
orientation: inline

BBa_K1486008
type: ComponentDefinition
persistent identity: BBa K1486008
display id: BBa_K1486008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CxpR & Split IFP1.4 [Cterm + Cterm][1]
creator: iGem EPFL 2014
created: 2014-08-25T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part has two fused proteins downstream of an arabinose-inducible promoter: CpxR-IFP[1] and CpxR-IFP[2]. This construct was made in all orientations: - IFP[1]-CpxR + IFP[2]-CpxR (BBa_K1286009) - IFP[1]-CpxR + CpxR-IFP[2] (BBa_K1486010) - CpxR-IFP[1] + IFP[2]-CpxR (BBa_K1486011)
mutable notes: All orientations were made, so all dimerizations could be tested (see main page for biobrick numbers). Flexible linkers were used for each of the fused proteins, and an RBS was put before each one of them.
mutable provenance: From Michnick Article. To Edit
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0034
component: BBa_K1486007
component: BBa_K1486004
component: BBa_K1486000
component: BBa_K1486052
component: BBa_K1486006
component: BBa_I0500
sequence annotation: BBa_I0500
sequence annotation: BBa_K1486052
sequence annotation: BBa_K1486000
sequence annotation: BBa_K1486004
sequence annotation: BBa_B0034
sequence annotation: BBa_K1486006
sequence annotation: BBa_K1486007
sequence: https://synbiohub.org/public/igem/BBa_K1486008_sequence/1

BBa_B0034
type: Component
persistent identity: component2418036
display id: component2418036
version: 1
top level: BBa_K1486008
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K1486007
type: Component
persistent identity: component2418057
display id: component2418057
version: 1
top level: BBa_K1486008
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486007/1

BBa_K1486004
type: Component
persistent identity: component2418041
display id: component2418041
version: 1
top level: BBa_K1486008
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486004/1

BBa_K1486000
type: Component
persistent identity: component2418040
display id: component2418040
version: 1
top level: BBa_K1486008
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486000/1

BBa_K1486004
type: Component
persistent identity: component2418053
display id: component2418053
version: 1
top level: BBa_K1486008
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486004/1

BBa_K1486052
type: Component
persistent identity: component2418046
display id: component2418046
version: 1
top level: BBa_K1486008
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486052/1

BBa_K1486006
type: Component
persistent identity: component2418045
display id: component2418045
version: 1
top level: BBa_K1486008
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486006/1

BBa_I0500
type: Component
persistent identity: component2418034
display id: component2418034
version: 1
top level: BBa_K1486008
access: public
definition: https://synbiohub.org/public/igem/BBa_I0500/1

BBa_K1486000
type: Component
persistent identity: component2418052
display id: component2418052
version: 1
top level: BBa_K1486008
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486000/1

BBa_B0034
type: Component
persistent identity: component2418048
display id: component2418048
version: 1
top level: BBa_K1486008
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_I0500
type: SequenceAnnotation
persistent identity: annotation2418034
display id: annotation2418034
version: 1
top level: BBa_K1486008
location: https://synbiohub.org/public/igem/BBa_K1486008/annotation2418034/range2418034/1
component: BBa_I0500

https://synbiohub.org/public/igem/BBa_K1486008/annotation2418034/range2418034/1
type: Range
persistent identity: range2418034
display id: range2418034
version: 1
direction: direction/reverse
top level: BBa_K1486008
start: 1
end: 1210
orientation: inline

BBa_K1486052
type: SequenceAnnotation
persistent identity: annotation2418046
display id: annotation2418046
version: 1
top level: BBa_K1486008
location: https://synbiohub.org/public/igem/BBa_K1486008/annotation2418046/range2418046/1
component: BBa_K1486052

https://synbiohub.org/public/igem/BBa_K1486008/annotation2418046/range2418046/1
type: Range
persistent identity: range2418046
display id: range2418046
version: 1
direction: direction/reverse
top level: BBa_K1486008
start: 2360
end: 2397
orientation: inline

BBa_K1486000
type: SequenceAnnotation
persistent identity: annotation2418040
display id: annotation2418040
version: 1
top level: BBa_K1486008
location: https://synbiohub.org/public/igem/BBa_K1486008/annotation2418040/range2418040/1
component: BBa_K1486000

https://synbiohub.org/public/igem/BBa_K1486008/annotation2418040/range2418040/1
type: Range
persistent identity: range2418040
display id: range2418040
version: 1
direction: direction/reverse
top level: BBa_K1486008
start: 1223
end: 1924
orientation: inline

BBa_K1486000
type: SequenceAnnotation
persistent identity: annotation2418052
display id: annotation2418052
version: 1
top level: BBa_K1486008
location: https://synbiohub.org/public/igem/BBa_K1486008/annotation2418052/range2418052/1
component: BBa_K1486000

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type: Range
persistent identity: range2418052
display id: range2418052
version: 1
direction: direction/reverse
top level: BBa_K1486008
start: 2410
end: 3111
orientation: inline

BBa_K1486004
type: SequenceAnnotation
persistent identity: annotation2418053
display id: annotation2418053
version: 1
top level: BBa_K1486008
location: https://synbiohub.org/public/igem/BBa_K1486008/annotation2418053/range2418053/1
component: BBa_K1486004

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type: Range
persistent identity: range2418053
display id: range2418053
version: 1
direction: direction/reverse
top level: BBa_K1486008
start: 3112
end: 3141
orientation: inline

BBa_K1486004
type: SequenceAnnotation
persistent identity: annotation2418041
display id: annotation2418041
version: 1
top level: BBa_K1486008
location: https://synbiohub.org/public/igem/BBa_K1486008/annotation2418041/range2418041/1
component: BBa_K1486004

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type: Range
persistent identity: range2418041
display id: range2418041
version: 1
direction: direction/reverse
top level: BBa_K1486008
start: 1925
end: 1954
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2418036
display id: annotation2418036
version: 1
top level: BBa_K1486008
location: https://synbiohub.org/public/igem/BBa_K1486008/annotation2418036/range2418036/1
component: BBa_B0034

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type: Range
persistent identity: range2418036
display id: range2418036
version: 1
direction: direction/reverse
top level: BBa_K1486008
start: 1211
end: 1222
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2418048
display id: annotation2418048
version: 1
top level: BBa_K1486008
location: https://synbiohub.org/public/igem/BBa_K1486008/annotation2418048/range2418048/1
component: BBa_B0034

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type: Range
persistent identity: range2418048
display id: range2418048
version: 1
direction: direction/reverse
top level: BBa_K1486008
start: 2398
end: 2409
orientation: inline

BBa_K1486006
type: SequenceAnnotation
persistent identity: annotation2418045
display id: annotation2418045
version: 1
top level: BBa_K1486008
location: https://synbiohub.org/public/igem/BBa_K1486008/annotation2418045/range2418045/1
component: BBa_K1486006

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type: Range
persistent identity: range2418045
display id: range2418045
version: 1
direction: direction/reverse
top level: BBa_K1486008
start: 1955
end: 2359
orientation: inline

BBa_K1486007
type: SequenceAnnotation
persistent identity: annotation2418057
display id: annotation2418057
version: 1
top level: BBa_K1486008
location: https://synbiohub.org/public/igem/BBa_K1486008/annotation2418057/range2418057/1
component: BBa_K1486007

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type: Range
persistent identity: range2418057
display id: range2418057
version: 1
direction: direction/reverse
top level: BBa_K1486008
start: 3142
end: 3726
orientation: inline

BBa_K1520020
type: ComponentDefinition
persistent identity: BBa K1520020
display id: BBa_K1520020
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Pcons2-rbs-lacI-Ter-Plac-rbs-luxI-Ter-Pcons2-rbs-luxR-Ter
creator: yue wu
created: 2014-09-21T11:00:00Z
modified: 2015-05-08T01:10:49Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1902_
m user id: 0
owner id: 21318
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Pcons2-rbs-lacI-Ter-Plac-rbs-luxI-Ter It produces luxI when there is enough IPTG.
mutable notes: None
mutable provenance: Pcons2, lacI, Plac, luxI, Ter
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1520019
component: BBa_K1520003
sequence annotation: BBa_K1520003
sequence annotation: BBa_K1520019
sequence: https://synbiohub.org/public/igem/BBa_K1520020_sequence/1

BBa_K1520019
type: Component
persistent identity: component2385110
display id: component2385110
version: 1
top level: BBa_K1520020
access: public
definition: https://synbiohub.org/public/igem/BBa_K1520019/1

BBa_K1520003
type: Component
persistent identity: component2385116
display id: component2385116
version: 1
top level: BBa_K1520020
access: public
definition: BBa_K1520003

BBa_K1520003
type: SequenceAnnotation
persistent identity: annotation2385116
display id: annotation2385116
version: 1
top level: BBa_K1520020
location: https://synbiohub.org/public/igem/BBa_K1520020/annotation2385116/range2385116/1
component: BBa_K1520003

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type: Range
persistent identity: range2385116
display id: range2385116
version: 1
direction: direction/reverse
top level: BBa_K1520020
start: 2374
end: 3352
orientation: inline

BBa_K1520019
type: SequenceAnnotation
persistent identity: annotation2385110
display id: annotation2385110
version: 1
top level: BBa_K1520020
location: https://synbiohub.org/public/igem/BBa_K1520020/annotation2385110/range2385110/1
component: BBa_K1520019

https://synbiohub.org/public/igem/BBa_K1520020/annotation2385110/range2385110/1
type: Range
persistent identity: range2385110
display id: range2385110
version: 1
direction: direction/reverse
top level: BBa_K1520020
start: 1
end: 2365
orientation: inline

BBa_K1486015
type: ComponentDefinition
persistent identity: BBa K1486015
display id: BBa_K1486015
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: IFP[2]-CpxR under pBAD
creator: EPFL iGem team 2014
created: 2014-09-09T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part has a fused protein downstream of an arabinose-inducible promoter: IFP[2]-CpxR. Biobrick BBa_K1486014 has IFP[1]-CpxR with an arabinose promoter. This construct was made in both orientations: BBa_K1486013 is CpxR-IFP[2] (with an arabinose promoter).
mutable notes: Both orientations were made, as well as for IFP[1] so all dimerizations could be tested (see main page for biobrick numbers). Flexible linkers were used to fuse both proteins, and an RBS was put before each one of them.
mutable provenance: To edit.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0034
component: BBa_K1486007
component: BBa_I0500
component: BBa_K1486004
component: BBa_K1486000
sequence annotation: BBa_I0500
sequence annotation: BBa_K1486000
sequence annotation: BBa_K1486007
sequence annotation: BBa_K1486004
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K1486015_sequence/1

BBa_B0034
type: Component
persistent identity: component2382850
display id: component2382850
version: 1
top level: BBa_K1486015
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K1486007
type: Component
persistent identity: component2382854
display id: component2382854
version: 1
top level: BBa_K1486015
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486007/1

BBa_I0500
type: Component
persistent identity: component2382848
display id: component2382848
version: 1
top level: BBa_K1486015
access: public
definition: https://synbiohub.org/public/igem/BBa_I0500/1

BBa_K1486004
type: Component
persistent identity: component2382855
display id: component2382855
version: 1
top level: BBa_K1486015
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486004/1

BBa_K1486000
type: Component
persistent identity: component2382859
display id: component2382859
version: 1
top level: BBa_K1486015
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486000/1

BBa_I0500
type: SequenceAnnotation
persistent identity: annotation2382848
display id: annotation2382848
version: 1
top level: BBa_K1486015
location: https://synbiohub.org/public/igem/BBa_K1486015/annotation2382848/range2382848/1
component: BBa_I0500

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type: Range
persistent identity: range2382848
display id: range2382848
version: 1
direction: direction/reverse
top level: BBa_K1486015
start: 1
end: 1210
orientation: inline

BBa_K1486000
type: SequenceAnnotation
persistent identity: annotation2382859
display id: annotation2382859
version: 1
top level: BBa_K1486015
location: https://synbiohub.org/public/igem/BBa_K1486015/annotation2382859/range2382859/1
component: BBa_K1486000

https://synbiohub.org/public/igem/BBa_K1486015/annotation2382859/range2382859/1
type: Range
persistent identity: range2382859
display id: range2382859
version: 1
direction: direction/reverse
top level: BBa_K1486015
start: 1860
end: 2561
orientation: inline

BBa_K1486007
type: SequenceAnnotation
persistent identity: annotation2382854
display id: annotation2382854
version: 1
top level: BBa_K1486015
location: https://synbiohub.org/public/igem/BBa_K1486015/annotation2382854/range2382854/1
component: BBa_K1486007

https://synbiohub.org/public/igem/BBa_K1486015/annotation2382854/range2382854/1
type: Range
persistent identity: range2382854
display id: range2382854
version: 1
direction: direction/reverse
top level: BBa_K1486015
start: 1237
end: 1815
orientation: inline

BBa_K1486004
type: SequenceAnnotation
persistent identity: annotation2382855
display id: annotation2382855
version: 1
top level: BBa_K1486015
location: https://synbiohub.org/public/igem/BBa_K1486015/annotation2382855/range2382855/1
component: BBa_K1486004

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type: Range
persistent identity: range2382855
display id: range2382855
version: 1
direction: direction/reverse
top level: BBa_K1486015
start: 1824
end: 1853
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2382850
display id: annotation2382850
version: 1
top level: BBa_K1486015
location: https://synbiohub.org/public/igem/BBa_K1486015/annotation2382850/range2382850/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1486015/annotation2382850/range2382850/1
type: Range
persistent identity: range2382850
display id: range2382850
version: 1
direction: direction/reverse
top level: BBa_K1486015
start: 1219
end: 1230
orientation: inline

BBa_K1486002
type: ComponentDefinition
persistent identity: BBa K1486002
display id: BBa_K1486002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Arabinose promoter + sfGFP CpxR (Nterm)
creator: iGem EPFL 2014
created: 2014-08-11T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part is composed of an arabinose-inducible promoter for superfolded GFP attached by a linker to CpxR (on the N terminus of CpxR).
mutable notes: We had to test both attachments, N and C terminus of the CpxR.
mutable provenance: The arabinose promoter and the sfGFP were obtained from the registry, while the CpxR was obtained from the E coli genome.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_I0500
component: BBa_K1486003
component: BBa_I746916
component: BBa_K1486000
component: BBa_B0034
sequence annotation: BBa_I0500
sequence annotation: BBa_B0034
sequence annotation: BBa_K1486003
sequence annotation: BBa_I746916
sequence annotation: BBa_K1486000
sequence: https://synbiohub.org/public/igem/BBa_K1486002_sequence/1

BBa_I0500
type: Component
persistent identity: component2382057
display id: component2382057
version: 1
top level: BBa_K1486002
access: public
definition: https://synbiohub.org/public/igem/BBa_I0500/1

BBa_K1486003
type: Component
persistent identity: component2382064
display id: component2382064
version: 1
top level: BBa_K1486002
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486003/1

BBa_I746916
type: Component
persistent identity: component2382063
display id: component2382063
version: 1
top level: BBa_K1486002
access: public
definition: https://synbiohub.org/public/igem/BBa_I746916/1

BBa_K1486000
type: Component
persistent identity: component2382068
display id: component2382068
version: 1
top level: BBa_K1486002
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486000/1

BBa_B0034
type: Component
persistent identity: component2382059
display id: component2382059
version: 1
top level: BBa_K1486002
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_I0500
type: SequenceAnnotation
persistent identity: annotation2382057
display id: annotation2382057
version: 1
top level: BBa_K1486002
location: https://synbiohub.org/public/igem/BBa_K1486002/annotation2382057/range2382057/1
component: BBa_I0500

https://synbiohub.org/public/igem/BBa_K1486002/annotation2382057/range2382057/1
type: Range
persistent identity: range2382057
display id: range2382057
version: 1
direction: direction/reverse
top level: BBa_K1486002
start: 1
end: 1210
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2382059
display id: annotation2382059
version: 1
top level: BBa_K1486002
location: https://synbiohub.org/public/igem/BBa_K1486002/annotation2382059/range2382059/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1486002/annotation2382059/range2382059/1
type: Range
persistent identity: range2382059
display id: range2382059
version: 1
direction: direction/reverse
top level: BBa_K1486002
start: 1211
end: 1222
orientation: inline

BBa_K1486003
type: SequenceAnnotation
persistent identity: annotation2382064
display id: annotation2382064
version: 1
top level: BBa_K1486002
location: https://synbiohub.org/public/igem/BBa_K1486002/annotation2382064/range2382064/1
component: BBa_K1486003

https://synbiohub.org/public/igem/BBa_K1486002/annotation2382064/range2382064/1
type: Range
persistent identity: range2382064
display id: range2382064
version: 1
direction: direction/reverse
top level: BBa_K1486002
start: 1943
end: 1966
orientation: inline

BBa_I746916
type: SequenceAnnotation
persistent identity: annotation2382063
display id: annotation2382063
version: 1
top level: BBa_K1486002
location: https://synbiohub.org/public/igem/BBa_K1486002/annotation2382063/range2382063/1
component: BBa_I746916

https://synbiohub.org/public/igem/BBa_K1486002/annotation2382063/range2382063/1
type: Range
persistent identity: range2382063
display id: range2382063
version: 1
direction: direction/reverse
top level: BBa_K1486002
start: 1223
end: 1942
orientation: inline

BBa_K1486000
type: SequenceAnnotation
persistent identity: annotation2382068
display id: annotation2382068
version: 1
top level: BBa_K1486002
location: https://synbiohub.org/public/igem/BBa_K1486002/annotation2382068/range2382068/1
component: BBa_K1486000

https://synbiohub.org/public/igem/BBa_K1486002/annotation2382068/range2382068/1
type: Range
persistent identity: range2382068
display id: range2382068
version: 1
direction: direction/reverse
top level: BBa_K1486002
start: 1967
end: 2668
orientation: inline

BBa_P0454
type: ComponentDefinition
persistent identity: BBa P0454
display id: BBa_P0454
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS.HK022 cI LVA+.Term
creator: Reshma Shetty
created: 2005-06-29T11:00:00Z
modified: 2015-05-08T01:14:11Z
discontinued: false
dominant: false
experience: experience/None
group u list: _41_
m user id: 0
owner id: 126
owning group id: 6
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_C0050
component: BBa_B0011
component: BBa_B0012
component: BBa_B0034
sequence annotation: BBa_B0011
sequence annotation: BBa_B0012
sequence annotation: BBa_C0050
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_P0454_sequence/1

BBa_C0050
type: Component
persistent identity: component1558030
display id: component1558030
version: 1
top level: BBa_P0454
access: public
definition: cI HK022

BBa_B0011
type: Component
persistent identity: component1558050
display id: component1558050
version: 1
top level: BBa_P0454
access: public
definition: https://synbiohub.org/public/igem/BBa_B0011/1

BBa_B0012
type: Component
persistent identity: component1558042
display id: component1558042
version: 1
top level: BBa_P0454
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0034
type: Component
persistent identity: component1558015
display id: component1558015
version: 1
top level: BBa_P0454
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0011
type: SequenceAnnotation
persistent identity: annotation1558050
display id: annotation1558050
version: 1
top level: BBa_P0454
location: https://synbiohub.org/public/igem/BBa_P0454/annotation1558050/range1558050/1
component: BBa_B0011

https://synbiohub.org/public/igem/BBa_P0454/annotation1558050/range1558050/1
type: Range
persistent identity: range1558050
display id: range1558050
version: 1
direction: direction/forward
top level: BBa_P0454
start: 845
end: 890
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1558042
display id: annotation1558042
version: 1
top level: BBa_P0454
location: https://synbiohub.org/public/igem/BBa_P0454/annotation1558042/range1558042/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_P0454/annotation1558042/range1558042/1
type: Range
persistent identity: range1558042
display id: range1558042
version: 1
direction: direction/forward
top level: BBa_P0454
start: 796
end: 836
orientation: inline

BBa_C0050
type: SequenceAnnotation
persistent identity: annotation1558030
display id: annotation1558030
version: 1
top level: BBa_P0454
location: https://synbiohub.org/public/igem/BBa_P0454/annotation1558030/range1558030/1
component: BBa_C0050

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type: Range
persistent identity: range1558030
display id: range1558030
version: 1
direction: direction/forward
top level: BBa_P0454
start: 19
end: 762
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1558015
display id: annotation1558015
version: 1
top level: BBa_P0454
location: https://synbiohub.org/public/igem/BBa_P0454/annotation1558015/range1558015/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_P0454/annotation1558015/range1558015/1
type: Range
persistent identity: range1558015
display id: range1558015
version: 1
direction: direction/forward
top level: BBa_P0454
start: 1
end: 12
orientation: inline

BBa_P0151
type: ComponentDefinition
persistent identity: BBa P0151
display id: BBa_P0151
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PoPS -> cI (lambda) [S0165]
creator: Randy Rettberg
created: 2004-04-24T11:00:00Z
modified: 2015-05-08T01:14:10Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Protein generator converting TIPS to the protein cI from lambda. Used as the input section for Quad Part Inverters Q01150 and Q01151.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0012
component: BBa_B0031
component: BBa_C0051
component: BBa_B0010
sequence annotation: BBa_B0031
sequence annotation: BBa_B0012
sequence annotation: BBa_C0051
sequence annotation: BBa_B0010
sequence: https://synbiohub.org/public/igem/BBa_P0151_sequence/1

BBa_B0012
type: Component
persistent identity: component944529
display id: component944529
version: 1
top level: BBa_P0151
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0031
type: Component
persistent identity: component944503
display id: component944503
version: 1
top level: BBa_P0151
access: public
definition: https://synbiohub.org/public/igem/BBa_B0031/1

BBa_C0051
type: Component
persistent identity: component944513
display id: component944513
version: 1
top level: BBa_P0151
access: public
definition: cI lam

BBa_B0010
type: Component
persistent identity: component944519
display id: component944519
version: 1
top level: BBa_P0151
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0031
type: SequenceAnnotation
persistent identity: annotation944503
display id: annotation944503
version: 1
top level: BBa_P0151
location: https://synbiohub.org/public/igem/BBa_P0151/annotation944503/range944503/1
component: BBa_B0031

https://synbiohub.org/public/igem/BBa_P0151/annotation944503/range944503/1
type: Range
persistent identity: range944503
display id: range944503
version: 1
direction: direction/forward
top level: BBa_P0151
start: 1
end: 14
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation944529
display id: annotation944529
version: 1
top level: BBa_P0151
location: https://synbiohub.org/public/igem/BBa_P0151/annotation944529/range944529/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_P0151/annotation944529/range944529/1
type: Range
persistent identity: range944529
display id: range944529
version: 1
direction: direction/forward
top level: BBa_P0151
start: 892
end: 932
orientation: inline

BBa_C0051
type: SequenceAnnotation
persistent identity: annotation944513
display id: annotation944513
version: 1
top level: BBa_P0151
location: https://synbiohub.org/public/igem/BBa_P0151/annotation944513/range944513/1
component: BBa_C0051

https://synbiohub.org/public/igem/BBa_P0151/annotation944513/range944513/1
type: Range
persistent identity: range944513
display id: range944513
version: 1
direction: direction/forward
top level: BBa_P0151
start: 21
end: 770
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation944519
display id: annotation944519
version: 1
top level: BBa_P0151
location: https://synbiohub.org/public/igem/BBa_P0151/annotation944519/range944519/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_P0151/annotation944519/range944519/1
type: Range
persistent identity: range944519
display id: range944519
version: 1
direction: direction/forward
top level: BBa_P0151
start: 804
end: 883
orientation: inline

BBa_K091190
type: ComponentDefinition
persistent identity: BBa K091190
display id: BBa_K091190
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: plux/cI+RBS+lasI+RBS+Mnt+TT+pMnt/lac+RBS+lasI+RBS+cI+TT
creator: Xiao Zhu, Alicia Allen, Andrew Gordon
created: 2008-08-07T11:00:00Z
modified: 2015-05-08T01:08:39Z
discontinued: false
dominant: false
experience: experience/None
group u list: _191_
m user id: 0
owner id: 3130
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: send P-AI-1 signaling molecules out, using Mnt(or cI) protein repress the pMnt/lac( or plux/cI) hybrid promoter
mutable notes: .
mutable provenance: .
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_K091188
component: BBa_K091162
sequence annotation: BBa_K091162
sequence annotation: BBa_K091188
sequence: https://synbiohub.org/public/igem/BBa_K091190_sequence/1

BBa_K091188
type: Component
persistent identity: component2302037
display id: component2302037
version: 1
top level: BBa_K091190
access: public
definition: https://synbiohub.org/public/igem/BBa_K091188/1

BBa_K091162
type: Component
persistent identity: component2302013
display id: component2302013
version: 1
top level: BBa_K091190
access: public
definition: https://synbiohub.org/public/igem/BBa_K091162/1

BBa_K091162
type: SequenceAnnotation
persistent identity: annotation2302013
display id: annotation2302013
version: 1
top level: BBa_K091190
location: https://synbiohub.org/public/igem/BBa_K091190/annotation2302013/range2302013/1
component: BBa_K091162

https://synbiohub.org/public/igem/BBa_K091190/annotation2302013/range2302013/1
type: Range
persistent identity: range2302013
display id: range2302013
version: 1
direction: direction/reverse
top level: BBa_K091190
start: 1
end: 1168
orientation: inline

BBa_K091188
type: SequenceAnnotation
persistent identity: annotation2302037
display id: annotation2302037
version: 1
top level: BBa_K091190
location: https://synbiohub.org/public/igem/BBa_K091190/annotation2302037/range2302037/1
component: BBa_K091188

https://synbiohub.org/public/igem/BBa_K091190/annotation2302037/range2302037/1
type: Range
persistent identity: range2302037
display id: range2302037
version: 1
direction: direction/reverse
top level: BBa_K091190
start: 1177
end: 2802
orientation: inline

BBa_P0455
type: ComponentDefinition
persistent identity: BBa P0455
display id: BBa_P0455
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS.Lambda cI LVA+.Term
creator: Reshma Shetty
created: 2005-06-29T11:00:00Z
modified: 2015-05-08T01:14:11Z
discontinued: false
dominant: false
experience: experience/None
group u list: _41_
m user id: 0
owner id: 126
owning group id: 6
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0012
component: BBa_B0034
component: BBa_C0051
component: BBa_B0011
sequence annotation: BBa_C0051
sequence annotation: BBa_B0012
sequence annotation: BBa_B0034
sequence annotation: BBa_B0011
sequence: https://synbiohub.org/public/igem/BBa_P0455_sequence/1

BBa_B0012
type: Component
persistent identity: component1558083
display id: component1558083
version: 1
top level: BBa_P0455
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0034
type: Component
persistent identity: component1558063
display id: component1558063
version: 1
top level: BBa_P0455
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_C0051
type: Component
persistent identity: component1558073
display id: component1558073
version: 1
top level: BBa_P0455
access: public
definition: cI lam

BBa_B0011
type: Component
persistent identity: component1558091
display id: component1558091
version: 1
top level: BBa_P0455
access: public
definition: https://synbiohub.org/public/igem/BBa_B0011/1

BBa_C0051
type: SequenceAnnotation
persistent identity: annotation1558073
display id: annotation1558073
version: 1
top level: BBa_P0455
location: https://synbiohub.org/public/igem/BBa_P0455/annotation1558073/range1558073/1
component: BBa_C0051

https://synbiohub.org/public/igem/BBa_P0455/annotation1558073/range1558073/1
type: Range
persistent identity: range1558073
display id: range1558073
version: 1
direction: direction/forward
top level: BBa_P0455
start: 19
end: 768
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1558083
display id: annotation1558083
version: 1
top level: BBa_P0455
location: https://synbiohub.org/public/igem/BBa_P0455/annotation1558083/range1558083/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_P0455/annotation1558083/range1558083/1
type: Range
persistent identity: range1558083
display id: range1558083
version: 1
direction: direction/forward
top level: BBa_P0455
start: 802
end: 842
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1558063
display id: annotation1558063
version: 1
top level: BBa_P0455
location: https://synbiohub.org/public/igem/BBa_P0455/annotation1558063/range1558063/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_P0455/annotation1558063/range1558063/1
type: Range
persistent identity: range1558063
display id: range1558063
version: 1
direction: direction/forward
top level: BBa_P0455
start: 1
end: 12
orientation: inline

BBa_B0011
type: SequenceAnnotation
persistent identity: annotation1558091
display id: annotation1558091
version: 1
top level: BBa_P0455
location: https://synbiohub.org/public/igem/BBa_P0455/annotation1558091/range1558091/1
component: BBa_B0011

https://synbiohub.org/public/igem/BBa_P0455/annotation1558091/range1558091/1
type: Range
persistent identity: range1558091
display id: range1558091
version: 1
direction: direction/forward
top level: BBa_P0455
start: 851
end: 896
orientation: inline

BBa_K091138
type: ComponentDefinition
persistent identity: BBa K091138
display id: BBa_K091138
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS+lsrR
creator: Xiao Zhu
created: 2008-07-14T11:00:00Z
modified: 2015-05-08T01:08:38Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _191_
m user id: 0
owner id: 3130
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: lsrR coding gene with ribosome binding site B0034.
mutable notes: no.
mutable provenance: lsrR comes from E. coli MG1655
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_B0034
component: BBa_K091001
sequence annotation: BBa_K091001
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K091138_sequence/1

BBa_B0034
type: Component
persistent identity: component1967008
display id: component1967008
version: 1
top level: BBa_K091138
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K091001
type: Component
persistent identity: component1967009
display id: component1967009
version: 1
top level: BBa_K091138
access: public
definition: https://synbiohub.org/public/igem/BBa_K091001/1

BBa_K091001
type: SequenceAnnotation
persistent identity: annotation1967009
display id: annotation1967009
version: 1
top level: BBa_K091138
location: https://synbiohub.org/public/igem/BBa_K091138/annotation1967009/range1967009/1
component: BBa_K091001

https://synbiohub.org/public/igem/BBa_K091138/annotation1967009/range1967009/1
type: Range
persistent identity: range1967009
display id: range1967009
version: 1
direction: direction/reverse
top level: BBa_K091138
start: 19
end: 972
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1967008
display id: annotation1967008
version: 1
top level: BBa_K091138
location: https://synbiohub.org/public/igem/BBa_K091138/annotation1967008/range1967008/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K091138/annotation1967008/range1967008/1
type: Range
persistent identity: range1967008
display id: range1967008
version: 1
direction: direction/reverse
top level: BBa_K091138
start: 1
end: 12
orientation: inline

BBa_K142074
type: ComponentDefinition
persistent identity: BBa K142074
display id: BBa_K142074
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: lac-controlled GFP-LVA generator with constitutive lacI expression cassette
creator: Julius Rabl
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:10:22Z
discontinued: false
dominant: false
group u list: _194_
m user id: 0
owner id: 3254
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: lac-controlled GFP-LVA generator with constitutive lacI expression cassette
mutable notes: We put the LacI generator behind the lac-controlled expression cassette to minimze readthrough.
mutable provenance: Subcloned from BioBricks using standard molecular biology techniques.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_I739002
component: BBa_I763004
sequence annotation: BBa_I739002
sequence annotation: BBa_I763004
sequence: https://synbiohub.org/public/igem/BBa_K142074_sequence/1

BBa_I739002
type: Component
persistent identity: component2283010
display id: component2283010
version: 1
top level: BBa_K142074
access: public
definition: https://synbiohub.org/public/igem/BBa_I739002/1

BBa_I763004
type: Component
persistent identity: component2282995
display id: component2282995
version: 1
top level: BBa_K142074
access: public
definition: https://synbiohub.org/public/igem/BBa_I763004/1

BBa_I739002
type: SequenceAnnotation
persistent identity: annotation2283010
display id: annotation2283010
version: 1
top level: BBa_K142074
location: https://synbiohub.org/public/igem/BBa_K142074/annotation2283010/range2283010/1
component: BBa_I739002

https://synbiohub.org/public/igem/BBa_K142074/annotation2283010/range2283010/1
type: Range
persistent identity: range2283010
display id: range2283010
version: 1
direction: direction/reverse
top level: BBa_K142074
start: 1131
end: 2481
orientation: inline

BBa_I763004
type: SequenceAnnotation
persistent identity: annotation2282995
display id: annotation2282995
version: 1
top level: BBa_K142074
location: https://synbiohub.org/public/igem/BBa_K142074/annotation2282995/range2282995/1
component: BBa_I763004

https://synbiohub.org/public/igem/BBa_K142074/annotation2282995/range2282995/1
type: Range
persistent identity: range2282995
display id: range2282995
version: 1
direction: direction/reverse
top level: BBa_K142074
start: 1
end: 1122
orientation: inline

cI 434
type: ComponentDefinition
persistent identity: BBa C0052
display id: BBa_C0052
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: cI repressor from phage 434 (+LVA)
creator: Maia Mahoney
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:23Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: The 434 cI repressor protein coding sequence is a 710 base-pair sequence with the standard RBS-compatible BioBrick prefix and the standard BioBrick suffix sections on its ends. It binds to the 434 regulatory sequence, BBa_R0052. The sequence contains a LVA tag for faster degredation and has no RBS.
mutable notes: References (unparsed) here: Nikolnikov,S., Posfai,G. and Sain,B. The construction of a versatile plasmid vector that allows direct selection of fragments cloned into six unique sites of the cI gene of coliphage 434. Gene 30 (1-3), 261-265 (1984) References (unparsed) here: Nikolnikov,S., Posfai,G. and Sain,B. The construction of a versatile plasmid vector that allows direct selection of fragments cloned into six unique sites of the cI gene of coliphage 434. Gene 30 (1-3), 261-265 (1984)
mutable provenance: Bacteriophage 434
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: LVA
sequence annotation: BBa_C0052
sequence annotation: Help:Barcodes
sequence annotation: cI 434
sequence: https://synbiohub.org/public/igem/BBa_C0052_sequence/1

LVA
type: SequenceAnnotation
persistent identity: annotation1745
display id: annotation1745
version: 1
top level: cI 434
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_C0052/annotation1745/range1745/1

https://synbiohub.org/public/igem/BBa_C0052/annotation1745/range1745/1
type: Range
persistent identity: range1745
display id: range1745
version: 1
direction: direction/forward
top level: cI 434
start: 631
end: 669
orientation: inline

BBa_C0052
type: SequenceAnnotation
persistent identity: annotation7035
display id: annotation7035
version: 1
top level: cI 434
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_C0052/annotation7035/range7035/1

https://synbiohub.org/public/igem/BBa_C0052/annotation7035/range7035/1
type: Range
persistent identity: range7035
display id: range7035
version: 1
direction: direction/forward
top level: cI 434
start: 1
end: 669
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213992
display id: annotation2213992
version: 1
top level: cI 434
role: SO:0000807
role: feature/barcode
location: https://synbiohub.org/public/igem/BBa_C0052/annotation2213992/range2213992/1

https://synbiohub.org/public/igem/BBa_C0052/annotation2213992/range2213992/1
type: Range
persistent identity: range2213992
display id: range2213992
version: 1
direction: direction/forward
top level: cI 434
start: 670
end: 694
orientation: inline

cI 434
type: SequenceAnnotation
persistent identity: annotation1743
display id: annotation1743
version: 1
top level: cI 434
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_C0052/annotation1743/range1743/1

https://synbiohub.org/public/igem/BBa_C0052/annotation1743/range1743/1
type: Range
persistent identity: range1743
display id: range1743
version: 1
direction: direction/forward
top level: cI 434
start: 1
end: 669
orientation: inline

BBa_K772005
type: ComponentDefinition
persistent identity: BBa K772005
display id: BBa_K772005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: AHL generator: LasI
creator: Mustafa Elitok
created: 2012-09-24T11:00:00Z
modified: 2015-05-08T01:13:15Z
discontinued: false
dominant: false
group u list: _1024_
m user id: 0
owner id: 9573
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Quorum sensing is the mechanism of communication between bacteria via synthesizing and detecting chemical compounds in the media. With these mediators, the promoters and their yield can be tuned or regulated. This part represents an alternative quorum sensing sender device which is designed to induce PoPS production in the parts including pLas such as BBa_K772004. LasI is an enzyme generator gene sequences which synthesizes 6HSLO3 (AHL) in order to induce a PoPS response in the pLas promoter. pLas promoter works in the presence of both 6HSLO3 and LasR protein.
mutable notes: Note that there is an RBS sequence just before LasI; in order to be easily used with quorum sensing receiver devices such as BBa_F2620 or BBa_K772004.
mutable provenance: LasI sequence is gathered from Registry.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: LasI
sequence annotation: Terminator
sequence annotation: pLac
sequence annotation: LacI
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_K772005_sequence/1

LasI
type: SequenceAnnotation
persistent identity: annotation2329745
display id: annotation2329745
version: 1
top level: BBa_K772005
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K772005/annotation2329745/range2329745/1

https://synbiohub.org/public/igem/BBa_K772005/annotation2329745/range2329745/1
type: Range
persistent identity: range2329745
display id: range2329745
version: 1
direction: direction/forward
top level: BBa_K772005
start: 1353
end: 1968
orientation: inline

Terminator
type: SequenceAnnotation
persistent identity: annotation2203715
display id: annotation2203715
version: 1
top level: BBa_K772005
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K772005/annotation2203715/range2203715/1

https://synbiohub.org/public/igem/BBa_K772005/annotation2203715/range2203715/1
type: Range
persistent identity: range2203715
display id: range2203715
version: 1
direction: direction/forward
top level: BBa_K772005
start: 1969
end: 2098
orientation: inline

pLac
type: SequenceAnnotation
persistent identity: annotation2203713
display id: annotation2203713
version: 1
top level: BBa_K772005
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K772005/annotation2203713/range2203713/1

https://synbiohub.org/public/igem/BBa_K772005/annotation2203713/range2203713/1
type: Range
persistent identity: range2203713
display id: range2203713
version: 1
direction: direction/forward
top level: BBa_K772005
start: 1133
end: 1331
orientation: inline

LacI
type: SequenceAnnotation
persistent identity: annotation2203712
display id: annotation2203712
version: 1
top level: BBa_K772005
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K772005/annotation2203712/range2203712/1

https://synbiohub.org/public/igem/BBa_K772005/annotation2203712/range2203712/1
type: Range
persistent identity: range2203712
display id: range2203712
version: 1
direction: direction/forward
top level: BBa_K772005
start: 1
end: 1132
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2203714
display id: annotation2203714
version: 1
top level: BBa_K772005
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K772005/annotation2203714/range2203714/1

https://synbiohub.org/public/igem/BBa_K772005/annotation2203714/range2203714/1
type: Range
persistent identity: range2203714
display id: range2203714
version: 1
direction: direction/forward
top level: BBa_K772005
start: 1332
end: 1352
orientation: inline

BBa_K165098
type: ComponentDefinition
persistent identity: BBa K165098
display id: BBa_K165098
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Zif268-HIV bs + ADH1 + Gli1 repressor (CFPx2 tagged) on pRS304*
creator: John Szymanski
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:10:57Z
discontinued: false
dominant: false
group u list: _267_
m user id: 0
owner id: 2512
owning group id: 58
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: -
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_K165003
component: BBa_K165007
component: BBa_K165011
component: BBa_K165002
component: BBa_K165014
component: BBa_K165018
component: BBa_K165015
sequence annotation: BBa_K165018
sequence annotation: BBa_K165014
sequence annotation: BBa_K165003
sequence annotation: BBa_K165007
sequence annotation: BBa_K165011
sequence annotation: BBa_K165015
sequence annotation: BBa_K165002
sequence: https://synbiohub.org/public/igem/BBa_K165098_sequence/1

BBa_K165003
type: Component
persistent identity: component1997386
display id: component1997386
version: 1
top level: BBa_K165098
access: public
definition: https://synbiohub.org/public/igem/BBa_K165003/1

BBa_K165007
type: Component
persistent identity: component1997387
display id: component1997387
version: 1
top level: BBa_K165098
access: public
definition: https://synbiohub.org/public/igem/BBa_K165007/1

BBa_K165011
type: Component
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display id: component1997383
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BBa_K165002
type: Component
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BBa_K165014
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BBa_K165018
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BBa_K165015
type: Component
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display id: component1997384
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definition: https://synbiohub.org/public/igem/BBa_K165015/1

BBa_K165018
type: SequenceAnnotation
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display id: annotation1997389
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BBa_K165003
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BBa_K165007
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BBa_K165011
type: SequenceAnnotation
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BBa_K165015
type: SequenceAnnotation
persistent identity: annotation1997384
display id: annotation1997384
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BBa_K165002
type: SequenceAnnotation
persistent identity: annotation1997385
display id: annotation1997385
version: 1
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component: BBa_K165002

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type: Range
persistent identity: range1997385
display id: range1997385
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Sen
type: ComponentDefinition
persistent identity: BBa I726041
display id: BBa_I726041
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Sen-TIC
creator: Bangalore iGEM
created: 2007-07-03T11:00:00Z
modified: 2015-08-31T04:07:55Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _107_
m user id: 0
owner id: 2077
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: Abcdefghijkl..
mutable notes: none
mutable provenance: Registry
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_R0040
component: BBa_E0020
component: BBa_B0012
component: BBa_C0161
component: BBa_B0034
component: BBa_B0010
sequence annotation: BBa_B0034
sequence annotation: BBa_E0020
sequence annotation: BBa_B0010
sequence annotation: BBa_R0040
sequence annotation: BBa_B0012
sequence annotation: BBa_C0161
sequence: https://synbiohub.org/public/igem/BBa_I726041_sequence/1

BBa_R0040
type: Component
persistent identity: component1951339
display id: component1951339
version: 1
top level: Sen
access: public
definition: https://synbiohub.org/public/igem/BBa_R0040/1

BBa_E0020
type: Component
persistent identity: component1951349
display id: component1951349
version: 1
top level: Sen
access: public
definition: https://synbiohub.org/public/igem/BBa_E0020/1

BBa_B0012
type: Component
persistent identity: component1951352
display id: component1951352
version: 1
top level: Sen
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_C0161
type: Component
persistent identity: component1951346
display id: component1951346
version: 1
top level: Sen
access: public
definition: https://synbiohub.org/public/igem/BBa_C0161/1

BBa_B0034
type: Component
persistent identity: component1951348
display id: component1951348
version: 1
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access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0034
type: Component
persistent identity: component1951344
display id: component1951344
version: 1
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access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0010
type: Component
persistent identity: component1951350
display id: component1951350
version: 1
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access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1951348
display id: annotation1951348
version: 1
top level: Sen
location: https://synbiohub.org/public/igem/BBa_I726041/annotation1951348/range1951348/1
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type: Range
persistent identity: range1951348
display id: range1951348
version: 1
direction: direction/reverse
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start: 674
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BBa_E0020
type: SequenceAnnotation
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display id: annotation1951349
version: 1
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persistent identity: range1951349
display id: range1951349
version: 1
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BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1951344
display id: annotation1951344
version: 1
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BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1951350
display id: annotation1951350
version: 1
top level: Sen
location: https://synbiohub.org/public/igem/BBa_I726041/annotation1951350/range1951350/1
component: BBa_B0010

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persistent identity: range1951350
display id: range1951350
version: 1
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BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1951339
display id: annotation1951339
version: 1
top level: Sen
location: https://synbiohub.org/public/igem/BBa_I726041/annotation1951339/range1951339/1
component: BBa_R0040

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type: Range
persistent identity: range1951339
display id: range1951339
version: 1
direction: direction/forward
top level: Sen
start: 1
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orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1951352
display id: annotation1951352
version: 1
top level: Sen
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type: Range
persistent identity: range1951352
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BBa_C0161
type: SequenceAnnotation
persistent identity: annotation1951346
display id: annotation1951346
version: 1
top level: Sen
location: https://synbiohub.org/public/igem/BBa_I726041/annotation1951346/range1951346/1
component: BBa_C0161

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type: Range
persistent identity: range1951346
display id: range1951346
version: 1
direction: direction/reverse
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start: 81
end: 665
orientation: inline

BBa_I712069
type: ComponentDefinition
persistent identity: BBa I712069
display id: BBa_I712069
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS-T7 RNA polymerase with NLS
creator: Sasa Jereb
created: 2007-10-21T11:00:00Z
modified: 2015-08-31T04:07:46Z
discontinued: false
dominant: true
experience: experience/None
group u list: _130_
m user id: 0
owner id: 1857
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: T7 RNA polymerase with RBS (ribosome-binding site) and NLS (nuclear localisation signal). NLS is used to target the protein to the cell nucleus.
mutable notes: DNA was isolated from BL21 and PCR was performed to attach RBS and NLS.
mutable provenance: Genomic DNA of BL21 (E.coli strain).
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
sequence: https://synbiohub.org/public/igem/BBa_I712069_sequence/1

TARS-LacI
type: ComponentDefinition
persistent identity: BBa K108030
display id: BBa_K108030
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TARS-LacI
creator: Shan Lin
created: 2008-10-22T11:00:00Z
modified: 2015-05-08T01:09:04Z
discontinued: false
dominant: false
experience: experience/None
group u list: _222_
m user id: 0
owner id: 2671
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: coming
mutable notes: ...
mutable provenance: ...
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
sequence annotation: LacI Operator
sequence annotation: LacI
sequence annotation: T7 Terminator
sequence annotation: TARS
sequence annotation: LVA
sequence: https://synbiohub.org/public/igem/BBa_K108030_sequence/1

LacI Operator
type: SequenceAnnotation
persistent identity: annotation1986398
display id: annotation1986398
version: 1
top level: TARS-LacI
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K108030/annotation1986398/range1986398/1

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type: Range
persistent identity: range1986398
display id: range1986398
version: 1
direction: direction/forward
top level: TARS-LacI
start: 4
end: 28
orientation: inline

LacI
type: SequenceAnnotation
persistent identity: annotation1986399
display id: annotation1986399
version: 1
top level: TARS-LacI
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K108030/annotation1986399/range1986399/1

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type: Range
persistent identity: range1986399
display id: range1986399
version: 1
direction: direction/forward
top level: TARS-LacI
start: 151
end: 1272
orientation: inline

T7 Terminator
type: SequenceAnnotation
persistent identity: annotation1986401
display id: annotation1986401
version: 1
top level: TARS-LacI
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K108030/annotation1986401/range1986401/1

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type: Range
persistent identity: range1986401
display id: range1986401
version: 1
direction: direction/forward
top level: TARS-LacI
start: 1279
end: 1326
orientation: inline

TARS
type: SequenceAnnotation
persistent identity: annotation1986400
display id: annotation1986400
version: 1
top level: TARS-LacI
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K108030/annotation1986400/range1986400/1

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type: Range
persistent identity: range1986400
display id: range1986400
version: 1
direction: direction/forward
top level: TARS-LacI
start: 29
end: 150
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation1986403
display id: annotation1986403
version: 1
top level: TARS-LacI
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K108030/annotation1986403/range1986403/1

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type: Range
persistent identity: range1986403
display id: range1986403
version: 1
direction: direction/forward
top level: TARS-LacI
start: 1240
end: 1278
orientation: inline

BBa_K165094
type: ComponentDefinition
persistent identity: BBa K165094
display id: BBa_K165094
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Gli1 bs + TEF2 + LexA repressor (CFPx2 tagged) on pRS304*
creator: John Szymanski
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:10:57Z
discontinued: false
dominant: false
group u list: _267_
m user id: 0
owner id: 2512
owning group id: 58
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: -
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_K165003
component: BBa_K165002
component: BBa_K165009
component: BBa_K165012
component: BBa_K165014
component: BBa_K165037
component: BBa_K165018
sequence annotation: BBa_K165009
sequence annotation: BBa_K165012
sequence annotation: BBa_K165037
sequence annotation: BBa_K165018
sequence annotation: BBa_K165002
sequence annotation: BBa_K165003
sequence annotation: BBa_K165014
sequence: https://synbiohub.org/public/igem/BBa_K165094_sequence/1

BBa_K165003
type: Component
persistent identity: component1997339
display id: component1997339
version: 1
top level: BBa_K165094
access: public
definition: https://synbiohub.org/public/igem/BBa_K165003/1

BBa_K165002
type: Component
persistent identity: component1997337
display id: component1997337
version: 1
top level: BBa_K165094
access: public
definition: https://synbiohub.org/public/igem/BBa_K165002/1

BBa_K165009
type: Component
persistent identity: component1997338
display id: component1997338
version: 1
top level: BBa_K165094
access: public
definition: https://synbiohub.org/public/igem/BBa_K165009/1

BBa_K165012
type: Component
persistent identity: component1997335
display id: component1997335
version: 1
top level: BBa_K165094
access: public
definition: https://synbiohub.org/public/igem/BBa_K165012/1

BBa_K165014
type: Component
persistent identity: component1997340
display id: component1997340
version: 1
top level: BBa_K165094
access: public
definition: https://synbiohub.org/public/igem/BBa_K165014/1

BBa_K165037
type: Component
persistent identity: component1997336
display id: component1997336
version: 1
top level: BBa_K165094
access: public
definition: https://synbiohub.org/public/igem/BBa_K165037/1

BBa_K165018
type: Component
persistent identity: component1997341
display id: component1997341
version: 1
top level: BBa_K165094
access: public
definition: https://synbiohub.org/public/igem/BBa_K165018/1

BBa_K165009
type: SequenceAnnotation
persistent identity: annotation1997338
display id: annotation1997338
version: 1
top level: BBa_K165094
location: https://synbiohub.org/public/igem/BBa_K165094/annotation1997338/range1997338/1
component: BBa_K165009

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type: Range
persistent identity: range1997338
display id: range1997338
version: 1
direction: direction/reverse
top level: BBa_K165094
start: 573
end: 830
orientation: inline

BBa_K165012
type: SequenceAnnotation
persistent identity: annotation1997335
display id: annotation1997335
version: 1
top level: BBa_K165094
location: https://synbiohub.org/public/igem/BBa_K165094/annotation1997335/range1997335/1
component: BBa_K165012

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type: Range
persistent identity: range1997335
display id: range1997335
version: 1
direction: direction/reverse
top level: BBa_K165094
start: 1
end: 127
orientation: inline

BBa_K165037
type: SequenceAnnotation
persistent identity: annotation1997336
display id: annotation1997336
version: 1
top level: BBa_K165094
location: https://synbiohub.org/public/igem/BBa_K165094/annotation1997336/range1997336/1
component: BBa_K165037

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type: Range
persistent identity: range1997336
display id: range1997336
version: 1
direction: direction/reverse
top level: BBa_K165094
start: 136
end: 538
orientation: inline

BBa_K165018
type: SequenceAnnotation
persistent identity: annotation1997341
display id: annotation1997341
version: 1
top level: BBa_K165094
location: https://synbiohub.org/public/igem/BBa_K165094/annotation1997341/range1997341/1
component: BBa_K165018

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type: Range
persistent identity: range1997341
display id: range1997341
version: 1
direction: direction/reverse
top level: BBa_K165094
start: 5160
end: 5412
orientation: inline

BBa_K165002
type: SequenceAnnotation
persistent identity: annotation1997337
display id: annotation1997337
version: 1
top level: BBa_K165094
location: https://synbiohub.org/public/igem/BBa_K165094/annotation1997337/range1997337/1
component: BBa_K165002

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type: Range
persistent identity: range1997337
display id: range1997337
version: 1
direction: direction/reverse
top level: BBa_K165094
start: 547
end: 564
orientation: inline

BBa_K165003
type: SequenceAnnotation
persistent identity: annotation1997339
display id: annotation1997339
version: 1
top level: BBa_K165094
location: https://synbiohub.org/public/igem/BBa_K165094/annotation1997339/range1997339/1
component: BBa_K165003

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type: Range
persistent identity: range1997339
display id: range1997339
version: 1
direction: direction/reverse
top level: BBa_K165094
start: 839
end: 3649
orientation: inline

BBa_K165014
type: SequenceAnnotation
persistent identity: annotation1997340
display id: annotation1997340
version: 1
top level: BBa_K165094
location: https://synbiohub.org/public/igem/BBa_K165094/annotation1997340/range1997340/1
component: BBa_K165014

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type: Range
persistent identity: range1997340
display id: range1997340
version: 1
direction: direction/reverse
top level: BBa_K165094
start: 3658
end: 5151
orientation: inline

TetR
type: ComponentDefinition
persistent identity: BBa I732084
display id: BBa_I732084
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Tet repressor generator (version1, TERM-)
creator: Zhan Jian
created: 2007-10-16T11:00:00Z
modified: 2015-08-31T04:07:57Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This part is similar to [http://partsregistry.org/wiki/index.php/Part:BBa_I732082 BBa_I732082], but there is a mutation induced by PCR. We are convinced that the mutation has nothing to do, and this part works as well as [http://partsregistry.org/wiki/index.php/Part:BBa_I732082 BBa_I732082].
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence: https://synbiohub.org/public/igem/BBa_I732084_sequence/1

BBa_K404105
type: ComponentDefinition
persistent identity: BBa K404105
display id: BBa_K404105
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: p40 promoter
creator: Freiburg Bioware 2010
created: 2010-08-27T11:00:00Z
modified: 2015-05-08T01:12:23Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 7049
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: p40 promoter
sequence: https://synbiohub.org/public/igem/BBa_K404105_sequence/1

p40 promoter
type: SequenceAnnotation
persistent identity: annotation2093170
display id: annotation2093170
version: 1
top level: BBa_K404105
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K404105/annotation2093170/range2093170/1

https://synbiohub.org/public/igem/BBa_K404105/annotation2093170/range2093170/1
type: Range
persistent identity: range2093170
display id: range2093170
version: 1
direction: direction/forward
top level: BBa_K404105
start: 1
end: 182
orientation: inline

BBa_K1031743
type: ComponentDefinition
persistent identity: BBa K1031743
display id: BBa_K1031743
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: J23113-PaaX
creator: He Shuaixin
created: 2013-09-08T11:00:00Z
modified: 2015-05-08T01:08:47Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1338_
m user id: 0
owner id: 15843
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: 113-PaaX-TT
mutable notes: molecular cloning
mutable provenance: PCR genome E.coli
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: BBa_B0034
sequence annotation: PaaX
sequence annotation: BBa_J23113
sequence: https://synbiohub.org/public/igem/BBa_K1031743_sequence/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2357492
display id: annotation2357492
version: 1
top level: BBa_K1031743
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1031743/annotation2357492/range2357492/1

https://synbiohub.org/public/igem/BBa_K1031743/annotation2357492/range2357492/1
type: Range
persistent identity: range2357492
display id: range2357492
version: 1
direction: direction/forward
top level: BBa_K1031743
start: 44
end: 55
orientation: inline

PaaX
type: SequenceAnnotation
persistent identity: annotation2357493
display id: annotation2357493
version: 1
top level: BBa_K1031743
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1031743/annotation2357493/range2357493/1

https://synbiohub.org/public/igem/BBa_K1031743/annotation2357493/range2357493/1
type: Range
persistent identity: range2357493
display id: range2357493
version: 1
direction: direction/forward
top level: BBa_K1031743
start: 62
end: 1012
orientation: inline

BBa_J23113
type: SequenceAnnotation
persistent identity: annotation2357491
display id: annotation2357491
version: 1
top level: BBa_K1031743
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1031743/annotation2357491/range2357491/1

https://synbiohub.org/public/igem/BBa_K1031743/annotation2357491/range2357491/1
type: Range
persistent identity: range2357491
display id: range2357491
version: 1
direction: direction/forward
top level: BBa_K1031743
start: 1
end: 35
orientation: inline

BBa_K1486014
type: ComponentDefinition
persistent identity: BBa K1486014
display id: BBa_K1486014
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: IFP[1]-CpxR under pBAD
creator: iGem EPFL 2014
created: 2014-08-31T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part has a fused protein downstream of an arabinose-inducible promoter: IFP[1]-CpxR. Biobrick BBa_K1486015 has IFP[2]-CpxR with an arabinose promoter. This construct was made in both orientations: BBa_K1486012 is CpxR-IFP[1] (with an arabinose promoter).
mutable notes: Both orientations were made, as well as for IFP[2] so all dimerizations could be tested (see main page for biobrick numbers). Flexible linkers were used to fuse both proteins, and an RBS was put between the promoter and the cds.
mutable provenance: to edit
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0034
component: BBa_K1486004
component: BBa_K1486000
component: BBa_I0500
component: BBa_K1486006
sequence annotation: BBa_K1486006
sequence annotation: BBa_I0500
sequence annotation: BBa_K1486004
sequence annotation: BBa_B0034
sequence annotation: BBa_K1486000
sequence: https://synbiohub.org/public/igem/BBa_K1486014_sequence/1

BBa_B0034
type: Component
persistent identity: component2424738
display id: component2424738
version: 1
top level: BBa_K1486014
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K1486004
type: Component
persistent identity: component2424743
display id: component2424743
version: 1
top level: BBa_K1486014
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486004/1

BBa_K1486000
type: Component
persistent identity: component2424747
display id: component2424747
version: 1
top level: BBa_K1486014
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486000/1

BBa_I0500
type: Component
persistent identity: component2424736
display id: component2424736
version: 1
top level: BBa_K1486014
access: public
definition: https://synbiohub.org/public/igem/BBa_I0500/1

BBa_K1486006
type: Component
persistent identity: component2424742
display id: component2424742
version: 1
top level: BBa_K1486014
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486006/1

BBa_K1486006
type: SequenceAnnotation
persistent identity: annotation2424742
display id: annotation2424742
version: 1
top level: BBa_K1486014
location: https://synbiohub.org/public/igem/BBa_K1486014/annotation2424742/range2424742/1
component: BBa_K1486006

https://synbiohub.org/public/igem/BBa_K1486014/annotation2424742/range2424742/1
type: Range
persistent identity: range2424742
display id: range2424742
version: 1
direction: direction/reverse
top level: BBa_K1486014
start: 1223
end: 1627
orientation: inline

BBa_I0500
type: SequenceAnnotation
persistent identity: annotation2424736
display id: annotation2424736
version: 1
top level: BBa_K1486014
location: https://synbiohub.org/public/igem/BBa_K1486014/annotation2424736/range2424736/1
component: BBa_I0500

https://synbiohub.org/public/igem/BBa_K1486014/annotation2424736/range2424736/1
type: Range
persistent identity: range2424736
display id: range2424736
version: 1
direction: direction/reverse
top level: BBa_K1486014
start: 1
end: 1210
orientation: inline

BBa_K1486004
type: SequenceAnnotation
persistent identity: annotation2424743
display id: annotation2424743
version: 1
top level: BBa_K1486014
location: https://synbiohub.org/public/igem/BBa_K1486014/annotation2424743/range2424743/1
component: BBa_K1486004

https://synbiohub.org/public/igem/BBa_K1486014/annotation2424743/range2424743/1
type: Range
persistent identity: range2424743
display id: range2424743
version: 1
direction: direction/reverse
top level: BBa_K1486014
start: 1628
end: 1657
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2424738
display id: annotation2424738
version: 1
top level: BBa_K1486014
location: https://synbiohub.org/public/igem/BBa_K1486014/annotation2424738/range2424738/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1486014/annotation2424738/range2424738/1
type: Range
persistent identity: range2424738
display id: range2424738
version: 1
direction: direction/reverse
top level: BBa_K1486014
start: 1211
end: 1222
orientation: inline

BBa_K1486000
type: SequenceAnnotation
persistent identity: annotation2424747
display id: annotation2424747
version: 1
top level: BBa_K1486014
location: https://synbiohub.org/public/igem/BBa_K1486014/annotation2424747/range2424747/1
component: BBa_K1486000

https://synbiohub.org/public/igem/BBa_K1486014/annotation2424747/range2424747/1
type: Range
persistent identity: range2424747
display id: range2424747
version: 1
direction: direction/reverse
top level: BBa_K1486014
start: 1658
end: 2359
orientation: inline

BBa_K165092
type: ComponentDefinition
persistent identity: BBa K165092
display id: BBa_K165092
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Zif268-HIV bs + LexA bs + mCYC + Gli1 repressor (mCherryx2 tagged) on pRS303
creator: John Szymanski
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:10:57Z
discontinued: false
dominant: false
group u list: _267_
m user id: 0
owner id: 2512
owning group id: 58
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: -
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_K165002
component: BBa_K165011
component: BBa_K165003
component: BBa_K165031
component: BBa_K165007
component: BBa_K165004
component: BBa_K165018
sequence annotation: BBa_K165002
sequence annotation: BBa_K165003
sequence annotation: BBa_K165007
sequence annotation: BBa_K165004
sequence annotation: BBa_K165011
sequence annotation: BBa_K165018
sequence annotation: BBa_K165031
sequence: https://synbiohub.org/public/igem/BBa_K165092_sequence/1

BBa_K165002
type: Component
persistent identity: component1997313
display id: component1997313
version: 1
top level: BBa_K165092
access: public
definition: https://synbiohub.org/public/igem/BBa_K165002/1

BBa_K165011
type: Component
persistent identity: component1997311
display id: component1997311
version: 1
top level: BBa_K165092
access: public
definition: https://synbiohub.org/public/igem/BBa_K165011/1

BBa_K165003
type: Component
persistent identity: component1997314
display id: component1997314
version: 1
top level: BBa_K165092
access: public
definition: https://synbiohub.org/public/igem/BBa_K165003/1

BBa_K165031
type: Component
persistent identity: component1997312
display id: component1997312
version: 1
top level: BBa_K165092
access: public
definition: https://synbiohub.org/public/igem/BBa_K165031/1

BBa_K165007
type: Component
persistent identity: component1997315
display id: component1997315
version: 1
top level: BBa_K165092
access: public
definition: https://synbiohub.org/public/igem/BBa_K165007/1

BBa_K165004
type: Component
persistent identity: component1997316
display id: component1997316
version: 1
top level: BBa_K165092
access: public
definition: https://synbiohub.org/public/igem/BBa_K165004/1

BBa_K165004
type: Component
persistent identity: component1997317
display id: component1997317
version: 1
top level: BBa_K165092
access: public
definition: https://synbiohub.org/public/igem/BBa_K165004/1

BBa_K165018
type: Component
persistent identity: component1997318
display id: component1997318
version: 1
top level: BBa_K165092
access: public
definition: https://synbiohub.org/public/igem/BBa_K165018/1

BBa_K165002
type: SequenceAnnotation
persistent identity: annotation1997313
display id: annotation1997313
version: 1
top level: BBa_K165092
location: https://synbiohub.org/public/igem/BBa_K165092/annotation1997313/range1997313/1
component: BBa_K165002

https://synbiohub.org/public/igem/BBa_K165092/annotation1997313/range1997313/1
type: Range
persistent identity: range1997313
display id: range1997313
version: 1
direction: direction/reverse
top level: BBa_K165092
start: 466
end: 483
orientation: inline

BBa_K165003
type: SequenceAnnotation
persistent identity: annotation1997314
display id: annotation1997314
version: 1
top level: BBa_K165092
location: https://synbiohub.org/public/igem/BBa_K165092/annotation1997314/range1997314/1
component: BBa_K165003

https://synbiohub.org/public/igem/BBa_K165092/annotation1997314/range1997314/1
type: Range
persistent identity: range1997314
display id: range1997314
version: 1
direction: direction/reverse
top level: BBa_K165092
start: 492
end: 3302
orientation: inline

BBa_K165007
type: SequenceAnnotation
persistent identity: annotation1997315
display id: annotation1997315
version: 1
top level: BBa_K165092
location: https://synbiohub.org/public/igem/BBa_K165092/annotation1997315/range1997315/1
component: BBa_K165007

https://synbiohub.org/public/igem/BBa_K165092/annotation1997315/range1997315/1
type: Range
persistent identity: range1997315
display id: range1997315
version: 1
direction: direction/reverse
top level: BBa_K165092
start: 3311
end: 3835
orientation: inline

BBa_K165004
type: SequenceAnnotation
persistent identity: annotation1997317
display id: annotation1997317
version: 1
top level: BBa_K165092
location: https://synbiohub.org/public/igem/BBa_K165092/annotation1997317/range1997317/1
component: BBa_K165004

https://synbiohub.org/public/igem/BBa_K165092/annotation1997317/range1997317/1
type: Range
persistent identity: range1997317
display id: range1997317
version: 1
direction: direction/reverse
top level: BBa_K165092
start: 4587
end: 5321
orientation: inline

BBa_K165011
type: SequenceAnnotation
persistent identity: annotation1997311
display id: annotation1997311
version: 1
top level: BBa_K165092
location: https://synbiohub.org/public/igem/BBa_K165092/annotation1997311/range1997311/1
component: BBa_K165011

https://synbiohub.org/public/igem/BBa_K165092/annotation1997311/range1997311/1
type: Range
persistent identity: range1997311
display id: range1997311
version: 1
direction: direction/reverse
top level: BBa_K165092
start: 1
end: 46
orientation: inline

BBa_K165018
type: SequenceAnnotation
persistent identity: annotation1997318
display id: annotation1997318
version: 1
top level: BBa_K165092
location: https://synbiohub.org/public/igem/BBa_K165092/annotation1997318/range1997318/1
component: BBa_K165018

https://synbiohub.org/public/igem/BBa_K165092/annotation1997318/range1997318/1
type: Range
persistent identity: range1997318
display id: range1997318
version: 1
direction: direction/reverse
top level: BBa_K165092
start: 5330
end: 5582
orientation: inline

BBa_K165004
type: SequenceAnnotation
persistent identity: annotation1997316
display id: annotation1997316
version: 1
top level: BBa_K165092
location: https://synbiohub.org/public/igem/BBa_K165092/annotation1997316/range1997316/1
component: BBa_K165004

https://synbiohub.org/public/igem/BBa_K165092/annotation1997316/range1997316/1
type: Range
persistent identity: range1997316
display id: range1997316
version: 1
direction: direction/reverse
top level: BBa_K165092
start: 3844
end: 4578
orientation: inline

BBa_K165031
type: SequenceAnnotation
persistent identity: annotation1997312
display id: annotation1997312
version: 1
top level: BBa_K165092
location: https://synbiohub.org/public/igem/BBa_K165092/annotation1997312/range1997312/1
component: BBa_K165031

https://synbiohub.org/public/igem/BBa_K165092/annotation1997312/range1997312/1
type: Range
persistent identity: range1997312
display id: range1997312
version: 1
direction: direction/reverse
top level: BBa_K165092
start: 55
end: 457
orientation: inline

BBa_K1031912
type: ComponentDefinition
persistent identity: BBa K1031912
display id: BBa_K1031912
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: J23105-XylS-Terminator
creator: He Shuaixin
created: 2013-09-08T11:00:00Z
modified: 2015-05-08T01:08:48Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1338_
m user id: 0
owner id: 15843
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: 105-XylS-TT
mutable notes: molecular cloning
mutable provenance: synthesis Pseudomonas putida
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: J23105
sequence annotation: B0015
sequence annotation: XylS
sequence: https://synbiohub.org/public/igem/BBa_K1031912_sequence/1

J23105
type: SequenceAnnotation
persistent identity: annotation2360063
display id: annotation2360063
version: 1
top level: BBa_K1031912
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1031912/annotation2360063/range2360063/1

https://synbiohub.org/public/igem/BBa_K1031912/annotation2360063/range2360063/1
type: Range
persistent identity: range2360063
display id: range2360063
version: 1
direction: direction/forward
top level: BBa_K1031912
start: 1
end: 35
orientation: inline

B0015
type: SequenceAnnotation
persistent identity: annotation2360108
display id: annotation2360108
version: 1
top level: BBa_K1031912
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1031912/annotation2360108/range2360108/1

https://synbiohub.org/public/igem/BBa_K1031912/annotation2360108/range2360108/1
type: Range
persistent identity: range2360108
display id: range2360108
version: 1
direction: direction/forward
top level: BBa_K1031912
start: 1034
end: 1162
orientation: inline

XylS
type: SequenceAnnotation
persistent identity: annotation2360107
display id: annotation2360107
version: 1
top level: BBa_K1031912
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1031912/annotation2360107/range2360107/1

https://synbiohub.org/public/igem/BBa_K1031912/annotation2360107/range2360107/1
type: Range
persistent identity: range2360107
display id: range2360107
version: 1
direction: direction/forward
top level: BBa_K1031912
start: 62
end: 1028
orientation: inline

BBa_K1520503
type: ComponentDefinition
persistent identity: BBa K1520503
display id: BBa_K1520503
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Rbs-cI(scarless)
creator: yue wu
created: 2014-09-21T11:00:00Z
modified: 2015-05-08T01:10:49Z
discontinued: false
dominant: false
group u list: _1902_
m user id: 0
owner id: 21318
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Rbs-cI(scarless) It is an improvement of cI(BBa_C0051) by adding a rbs.
mutable notes: None
mutable provenance: It is an improvement of cI(BBa_C0051) by adding a rbs.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
sequence annotation: cI
sequence annotation: rbs
sequence: https://synbiohub.org/public/igem/BBa_K1520503_sequence/1

cI
type: SequenceAnnotation
persistent identity: annotation2384868
display id: annotation2384868
version: 1
top level: BBa_K1520503
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1520503/annotation2384868/range2384868/1

https://synbiohub.org/public/igem/BBa_K1520503/annotation2384868/range2384868/1
type: Range
persistent identity: range2384868
display id: range2384868
version: 1
direction: direction/forward
top level: BBa_K1520503
start: 19
end: 793
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation2384867
display id: annotation2384867
version: 1
top level: BBa_K1520503
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1520503/annotation2384867/range2384867/1

https://synbiohub.org/public/igem/BBa_K1520503/annotation2384867/range2384867/1
type: Range
persistent identity: range2384867
display id: range2384867
version: 1
direction: direction/forward
top level: BBa_K1520503
start: 1
end: 18
orientation: inline

BBa_K082025
type: ComponentDefinition
persistent identity: BBa K082025
display id: BBa_K082025
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: B0034-LacI-B0015
creator: Yanting Xue , He Shen
created: 2008-10-28T12:00:00Z
modified: 2015-05-08T01:08:36Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _228_
m user id: 0
owner id: 1560
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: none
mutable notes: none
mutable provenance: none
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K082004
component: BBa_B0034
component: BBa_B0015
sequence annotation: BBa_B0015
sequence annotation: BBa_K082004
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K082025_sequence/1

BBa_K082004
type: Component
persistent identity: component2220466
display id: component2220466
version: 1
top level: BBa_K082025
access: public
definition: https://synbiohub.org/public/igem/BBa_K082004/1

BBa_B0034
type: Component
persistent identity: component2220465
display id: component2220465
version: 1
top level: BBa_K082025
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0015
type: Component
persistent identity: component2220473
display id: component2220473
version: 1
top level: BBa_K082025
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2220473
display id: annotation2220473
version: 1
top level: BBa_K082025
location: https://synbiohub.org/public/igem/BBa_K082025/annotation2220473/range2220473/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K082025/annotation2220473/range2220473/1
type: Range
persistent identity: range2220473
display id: range2220473
version: 1
direction: direction/reverse
top level: BBa_K082025
start: 1112
end: 1240
orientation: inline

BBa_K082004
type: SequenceAnnotation
persistent identity: annotation2220466
display id: annotation2220466
version: 1
top level: BBa_K082025
location: https://synbiohub.org/public/igem/BBa_K082025/annotation2220466/range2220466/1
component: BBa_K082004

https://synbiohub.org/public/igem/BBa_K082025/annotation2220466/range2220466/1
type: Range
persistent identity: range2220466
display id: range2220466
version: 1
direction: direction/reverse
top level: BBa_K082025
start: 21
end: 1103
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2220465
display id: annotation2220465
version: 1
top level: BBa_K082025
location: https://synbiohub.org/public/igem/BBa_K082025/annotation2220465/range2220465/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K082025/annotation2220465/range2220465/1
type: Range
persistent identity: range2220465
display id: range2220465
version: 1
direction: direction/reverse
top level: BBa_K082025
start: 1
end: 12
orientation: inline

BBa_I714892
type: ComponentDefinition
persistent identity: BBa I714892
display id: BBa_I714892
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CIind-
creator: Chunbo Lou
created: 2007-10-20T11:00:00Z
modified: 2015-08-31T04:07:48Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _142_
m user id: 0
owner id: 1911
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: we construct a CI mutant which is derivated from CI857(ind-)
mutable notes: make a CI mutant which is not degenerated by RecA*
mutable provenance: lambda genome
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
sequence annotation: SDA
sequence annotation: CIind-
sequence: https://synbiohub.org/public/igem/BBa_I714892_sequence/1

SDA
type: SequenceAnnotation
persistent identity: annotation1951564
display id: annotation1951564
version: 1
top level: BBa_I714892
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_I714892/annotation1951564/range1951564/1

https://synbiohub.org/public/igem/BBa_I714892/annotation1951564/range1951564/1
type: Range
persistent identity: range1951564
display id: range1951564
version: 1
direction: direction/forward
top level: BBa_I714892
start: 1
end: 12
orientation: inline

CIind-
type: SequenceAnnotation
persistent identity: annotation1951565
display id: annotation1951565
version: 1
top level: BBa_I714892
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_I714892/annotation1951565/range1951565/1

https://synbiohub.org/public/igem/BBa_I714892/annotation1951565/range1951565/1
type: Range
persistent identity: range1951565
display id: range1951565
version: 1
direction: direction/forward
top level: BBa_I714892
start: 13
end: 726
orientation: inline

CspC
type: ComponentDefinition
persistent identity: BBa K538004
display id: BBa_K538004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CspC (P. irgensii)
creator: Bas Stringer (Sequence by Paul van Dieken)
created: 2011-09-07T11:00:00Z
modified: 2015-05-08T01:12:37Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _704_
m user id: 0
owner id: 9985
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: -
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: A > G (Recode for SapI site)
sequence annotation: Start Codon
sequence annotation: RNA binding motif (RPN1)
sequence annotation: RNA binding motif (RPN2)
sequence annotation: Double Stop Codon
sequence: https://synbiohub.org/public/igem/BBa_K538004_sequence/1

A > G (Recode for SapI site)
type: SequenceAnnotation
persistent identity: annotation2127785
display id: annotation2127785
version: 1
top level: CspC
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_K538004/annotation2127785/range2127785/1

https://synbiohub.org/public/igem/BBa_K538004/annotation2127785/range2127785/1
type: Range
persistent identity: range2127785
display id: range2127785
version: 1
direction: direction/forward
top level: CspC
start: 75
end: 75
orientation: inline

Start Codon
type: SequenceAnnotation
persistent identity: annotation2127783
display id: annotation2127783
version: 1
top level: CspC
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K538004/annotation2127783/range2127783/1

https://synbiohub.org/public/igem/BBa_K538004/annotation2127783/range2127783/1
type: Range
persistent identity: range2127783
display id: range2127783
version: 1
direction: direction/forward
top level: CspC
start: 1
end: 3
orientation: inline

RNA binding motif (RPN1)
type: SequenceAnnotation
persistent identity: annotation2129605
display id: annotation2129605
version: 1
top level: CspC
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K538004/annotation2129605/range2129605/1

https://synbiohub.org/public/igem/BBa_K538004/annotation2129605/range2129605/1
type: Range
persistent identity: range2129605
display id: range2129605
version: 1
direction: direction/forward
top level: CspC
start: 37
end: 54
orientation: inline

RNA binding motif (RPN2)
type: SequenceAnnotation
persistent identity: annotation2129606
display id: annotation2129606
version: 1
top level: CspC
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K538004/annotation2129606/range2129606/1

https://synbiohub.org/public/igem/BBa_K538004/annotation2129606/range2129606/1
type: Range
persistent identity: range2129606
display id: range2129606
version: 1
direction: direction/forward
top level: CspC
start: 79
end: 93
orientation: inline

Double Stop Codon
type: SequenceAnnotation
persistent identity: annotation2127784
display id: annotation2127784
version: 1
top level: CspC
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K538004/annotation2127784/range2127784/1

https://synbiohub.org/public/igem/BBa_K538004/annotation2127784/range2127784/1
type: Range
persistent identity: range2127784
display id: range2127784
version: 1
direction: direction/forward
top level: CspC
start: 190
end: 195
orientation: inline

lexA
type: ComponentDefinition
persistent identity: BBa K165020
display id: BBa_K165020
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LexA Synthetic Activator (untagged)
creator: John Szymanski
created: 2008-10-27T12:00:00Z
modified: 2015-05-08T01:10:55Z
discontinued: false
dominant: false
experience: experience/None
group u list: _267_
m user id: 0
owner id: 2512
owning group id: 58
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This is a synthetic transcriptional activator for use in yeast. It contains the VP16 activation domain, the LexA DNA-binding domain, the SV40 nuclear localization sequence, and the ADH1 terminator. It binds to the Lex operator, activating transcription downstream.
mutable notes: -
mutable provenance: From David Drubin in Silver Lab at Harvard.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence: https://synbiohub.org/public/igem/BBa_K165020_sequence/1

zif23
type: ComponentDefinition
persistent identity: BBa C2001
display id: BBa_C2001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Zif23-GCN4 engineered repressor (+LVA, C2000 codon-optimized for E.coli)
creator: Drew Endy
created: 2004-01-29T12:00:00Z
modified: 2015-08-31T04:07:25Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Variant of Zif23-GCN4 (see BBa_C2000). CDS has been optimized (via VNTI) for expression in enteric bacteria. ssrA tag (LVA) added.
mutable notes: This protein should form a dimer and bind a specific operator site, thus repressing transcription.
mutable provenance: Protein Engineering Gods from the Pabo Lab
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Finger 2
sequence annotation: Recognition site
sequence annotation: GCN4: leucine zipper
sequence annotation: Finger 3
sequence annotation: LVA
sequence annotation: linker
sequence annotation: Help:Barcodes
sequence annotation: 2
sequence: https://synbiohub.org/public/igem/BBa_C2001_sequence/1

Finger 2
type: SequenceAnnotation
persistent identity: annotation318101
display id: annotation318101
version: 1
top level: zif23
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_C2001/annotation318101/range318101/1

https://synbiohub.org/public/igem/BBa_C2001/annotation318101/range318101/1
type: Range
persistent identity: range318101
display id: range318101
version: 1
direction: direction/forward
top level: zif23
start: 4
end: 90
orientation: inline

Recognition site
type: SequenceAnnotation
persistent identity: annotation318108
display id: annotation318108
version: 1
top level: zif23
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_C2001/annotation318108/range318108/1

https://synbiohub.org/public/igem/BBa_C2001/annotation318108/range318108/1
type: Range
persistent identity: range318108
display id: range318108
version: 1
direction: direction/forward
top level: zif23
start: 43
end: 63
orientation: inline

GCN4: leucine zipper
type: SequenceAnnotation
persistent identity: annotation318105
display id: annotation318105
version: 1
top level: zif23
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_C2001/annotation318105/range318105/1

https://synbiohub.org/public/igem/BBa_C2001/annotation318105/range318105/1
type: Range
persistent identity: range318105
display id: range318105
version: 1
direction: direction/forward
top level: zif23
start: 166
end: 261
orientation: inline

Finger 3
type: SequenceAnnotation
persistent identity: annotation318103
display id: annotation318103
version: 1
top level: zif23
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_C2001/annotation318103/range318103/1

https://synbiohub.org/public/igem/BBa_C2001/annotation318103/range318103/1
type: Range
persistent identity: range318103
display id: range318103
version: 1
direction: direction/forward
top level: zif23
start: 91
end: 150
orientation: inline

Recognition site
type: SequenceAnnotation
persistent identity: annotation318109
display id: annotation318109
version: 1
top level: zif23
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_C2001/annotation318109/range318109/1

https://synbiohub.org/public/igem/BBa_C2001/annotation318109/range318109/1
type: Range
persistent identity: range318109
display id: range318109
version: 1
direction: direction/forward
top level: zif23
start: 127
end: 147
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation318106
display id: annotation318106
version: 1
top level: zif23
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_C2001/annotation318106/range318106/1

https://synbiohub.org/public/igem/BBa_C2001/annotation318106/range318106/1
type: Range
persistent identity: range318106
display id: range318106
version: 1
direction: direction/forward
top level: zif23
start: 262
end: 294
orientation: inline

linker
type: SequenceAnnotation
persistent identity: annotation318104
display id: annotation318104
version: 1
top level: zif23
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_C2001/annotation318104/range318104/1

https://synbiohub.org/public/igem/BBa_C2001/annotation318104/range318104/1
type: Range
persistent identity: range318104
display id: range318104
version: 1
direction: direction/forward
top level: zif23
start: 151
end: 165
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2214011
display id: annotation2214011
version: 1
top level: zif23
role: SO:0000807
role: feature/barcode
location: https://synbiohub.org/public/igem/BBa_C2001/annotation2214011/range2214011/1

https://synbiohub.org/public/igem/BBa_C2001/annotation2214011/range2214011/1
type: Range
persistent identity: range2214011
display id: range2214011
version: 1
direction: direction/forward
top level: zif23
start: 301
end: 325
orientation: inline

2
type: SequenceAnnotation
persistent identity: annotation318107
display id: annotation318107
version: 1
top level: zif23
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_C2001/annotation318107/range318107/1

https://synbiohub.org/public/igem/BBa_C2001/annotation318107/range318107/1
type: Range
persistent identity: range318107
display id: range318107
version: 1
direction: direction/forward
top level: zif23
start: 295
end: 300
orientation: inline

BBa_K1666006
type: ComponentDefinition
persistent identity: BBa K1666006
display id: BBa_K1666006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: lsr promoter of LuxS/AI-2 signaling pathway in Salmonalla
creator: Heming Wang
created: 2015-09-05T11:00:00Z
modified: 2015-09-18T08:16:38Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2084_
m user id: 28478
owner id: 23948
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Quorum sensing is a process of bacterial cell-to-cell communication involving the production and detection of extracellular signaling molecules called autoinducers. And autoinducer-2 (AI-2) has been proposed to serve as a 'universal signal' for interspecies communication. In the LuxS/AI-2 signaling system of Salmonella Typhimurium, AI-2 response involves ATP binding cassette transporter encoded by genes named Lsr (LuxS regulated). And plsr is member of lsr operon which could be repressed by LsrR. In our project, we set this promoter part in front of a blue pigment as a reporter monitoring the presence of AI-2.
mutable notes: The lsrD gene was isolated from the genome of Salmonella enterica subsp. enterica serovar Typhimurium str. LT2 using PCR. We added restriction enzyme sites when designing primers, and the assembly is done through the use of restriction sites (cutting and ligating).
mutable provenance: We extracted the genomic DNA of Salmonella enterica subsp. enterica serovar Typhimurium str. LT2, and used PCR to isolate the promoter plsr.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: plsr
sequence: https://synbiohub.org/public/igem/BBa_K1666006_sequence/1

plsr
type: SequenceAnnotation
persistent identity: annotation2445166
display id: annotation2445166
version: 1
top level: BBa_K1666006
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1666006/annotation2445166/range2445166/1

https://synbiohub.org/public/igem/BBa_K1666006/annotation2445166/range2445166/1
type: Range
persistent identity: range2445166
display id: range2445166
version: 1
direction: direction/forward
top level: BBa_K1666006
start: 1
end: 255
orientation: inline

LacI:CspA
type: ComponentDefinition
persistent identity: BBa K538203
display id: BBa_K538203
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pLacI + RBS + CspA
creator: Bas Stringer
created: 2011-09-07T11:00:00Z
modified: 2015-05-08T01:12:37Z
discontinued: false
dominant: false
experience: experience/None
group u list: _704_
m user id: 0
owner id: 9985
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: -
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0034
component: BBa_K538003
component: BBa_R0010
sequence annotation: BBa_R0010
sequence annotation: BBa_B0034
sequence annotation: BBa_K538003
sequence: https://synbiohub.org/public/igem/BBa_K538203_sequence/1

BBa_B0034
type: Component
persistent identity: component2134066
display id: component2134066
version: 1
top level: LacI:CspA
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K538003
type: Component
persistent identity: component2134071
display id: component2134071
version: 1
top level: LacI:CspA
access: public
definition: CspA

BBa_R0010
type: Component
persistent identity: component2134058
display id: component2134058
version: 1
top level: LacI:CspA
access: public
definition: https://synbiohub.org/public/igem/BBa_R0010/1

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation2134058
display id: annotation2134058
version: 1
top level: LacI:CspA
location: https://synbiohub.org/public/igem/BBa_K538203/annotation2134058/range2134058/1
component: BBa_R0010

https://synbiohub.org/public/igem/BBa_K538203/annotation2134058/range2134058/1
type: Range
persistent identity: range2134058
display id: range2134058
version: 1
direction: direction/forward
top level: LacI:CspA
start: 1
end: 200
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2134066
display id: annotation2134066
version: 1
top level: LacI:CspA
location: https://synbiohub.org/public/igem/BBa_K538203/annotation2134066/range2134066/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K538203/annotation2134066/range2134066/1
type: Range
persistent identity: range2134066
display id: range2134066
version: 1
direction: direction/reverse
top level: LacI:CspA
start: 209
end: 220
orientation: inline

BBa_K538003
type: SequenceAnnotation
persistent identity: annotation2134071
display id: annotation2134071
version: 1
top level: LacI:CspA
location: https://synbiohub.org/public/igem/BBa_K538203/annotation2134071/range2134071/1
component: BBa_K538003

https://synbiohub.org/public/igem/BBa_K538203/annotation2134071/range2134071/1
type: Range
persistent identity: range2134071
display id: range2134071
version: 1
direction: direction/reverse
top level: LacI:CspA
start: 227
end: 421
orientation: inline

BBa_K1486046
type: ComponentDefinition
persistent identity: BBa K1486046
display id: BBa_K1486046
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CpxR promoter FW
creator: EPFL iGem team 2014
created: 2014-10-06T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1866_
m user id: 0
owner id: 21033
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Test
mutable notes: Test
mutable provenance: Test
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K1486046_sequence/1

BBa_K1031311
type: ComponentDefinition
persistent identity: BBa K1031311
display id: BBa_K1031311
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: J23114-HbpR-Terminator
creator: He Shuaixin
created: 2013-10-12T11:00:00Z
modified: 2015-05-08T01:08:47Z
discontinued: false
dominant: false
group u list: _1338_
m user id: 0
owner id: 15843
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: 114-HbpR-TT
mutable notes: molecular cloning
mutable provenance: P.putida
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: J23114
sequence annotation: HbpR
sequence annotation: B0015
sequence: https://synbiohub.org/public/igem/BBa_K1031311_sequence/1

J23114
type: SequenceAnnotation
persistent identity: annotation2369597
display id: annotation2369597
version: 1
top level: BBa_K1031311
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1031311/annotation2369597/range2369597/1

https://synbiohub.org/public/igem/BBa_K1031311/annotation2369597/range2369597/1
type: Range
persistent identity: range2369597
display id: range2369597
version: 1
direction: direction/forward
top level: BBa_K1031311
start: 1
end: 35
orientation: inline

HbpR
type: SequenceAnnotation
persistent identity: annotation2369598
display id: annotation2369598
version: 1
top level: BBa_K1031311
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1031311/annotation2369598/range2369598/1

https://synbiohub.org/public/igem/BBa_K1031311/annotation2369598/range2369598/1
type: Range
persistent identity: range2369598
display id: range2369598
version: 1
direction: direction/forward
top level: BBa_K1031311
start: 63
end: 1713
orientation: inline

B0015
type: SequenceAnnotation
persistent identity: annotation2369599
display id: annotation2369599
version: 1
top level: BBa_K1031311
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1031311/annotation2369599/range2369599/1

https://synbiohub.org/public/igem/BBa_K1031311/annotation2369599/range2369599/1
type: Range
persistent identity: range2369599
display id: range2369599
version: 1
direction: direction/forward
top level: BBa_K1031311
start: 1782
end: 1910
orientation: inline

BBa_I746350
type: ComponentDefinition
persistent identity: BBa I746350
display id: BBa_I746350
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: ogr activator from P2 phage
creator: Stefan Milde
created: 2007-09-10T11:00:00Z
modified: 2015-08-31T04:08:04Z
discontinued: false
dominant: false
experience: experience/None
group u list: _116_
m user id: 0
owner id: 2122
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: The ogr activator taken from P2 phage acts on a class of inducible promoters (parts I746360 to I746365), inducing their activity to varying degrees. The part sequence does already contain a ribosome binding site (B0034)!
mutable notes: The part does contain a RBS (B0034) already.
mutable provenance: plasmid DNA supplied by Prof. Richard Calendar, University of California.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
sequence annotation: P2 ogr
sequence annotation: B0034
sequence: https://synbiohub.org/public/igem/BBa_I746350_sequence/1

P2 ogr
type: SequenceAnnotation
persistent identity: annotation1943867
display id: annotation1943867
version: 1
top level: BBa_I746350
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_I746350/annotation1943867/range1943867/1

https://synbiohub.org/public/igem/BBa_I746350/annotation1943867/range1943867/1
type: Range
persistent identity: range1943867
display id: range1943867
version: 1
direction: direction/forward
top level: BBa_I746350
start: 19
end: 237
orientation: inline

B0034
type: SequenceAnnotation
persistent identity: annotation1943865
display id: annotation1943865
version: 1
top level: BBa_I746350
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_I746350/annotation1943865/range1943865/1

https://synbiohub.org/public/igem/BBa_I746350/annotation1943865/range1943865/1
type: Range
persistent identity: range1943865
display id: range1943865
version: 1
direction: direction/forward
top level: BBa_I746350
start: 1
end: 12
orientation: inline

P2 ogr
type: SequenceAnnotation
persistent identity: annotation1943866
display id: annotation1943866
version: 1
top level: BBa_I746350
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_I746350/annotation1943866/range1943866/1

https://synbiohub.org/public/igem/BBa_I746350/annotation1943866/range1943866/1
type: Range
persistent identity: range1943866
display id: range1943866
version: 1
direction: direction/forward
top level: BBa_I746350
start: 19
end: 19
orientation: inline

luxr
type: ComponentDefinition
persistent identity: BBa C0062
display id: BBa_C0062
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: luxR repressor/activator, (no LVA?)
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:23Z
discontinued: false
dominant: true
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: In complex with HSL, LuxR binds to the Lux promoter, activating transcription from Pr <bb_part>BBa_R0062</bb_part>, and repressing transcription from Pl <bb_part>BBa_R0063</bb_part>. The lux cassette of V. fischeri contains a left and a right promoter. The right promoter gives weak constitutive expression of downstream genes.This expression is up-regulated by the action of the Lux activator, LuxR complexed to HSL. Two molecules of LuxR protein form a complex with two molecules the signalling compound homoserine lactone (HSL). This complex binds to a palindromic site on the promoter, increasing the rate of transcription.
mutable notes: 2 silent point mutants were introduced in the coding sequence to remove internal XbaI and PstI sites. Mutation sites were chosen to replace codons commonly used in E. coli with codons used at a similar frequency.
mutable provenance: V. fischeri AF170104
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: A
sequence annotation: luxR
sequence annotation: Help:Barcodes
sequence annotation: T
sequence annotation: prefix
sequence annotation: BBa_C0062
sequence: https://synbiohub.org/public/igem/BBa_C0062_sequence/1

A
type: SequenceAnnotation
persistent identity: annotation1765
display id: annotation1765
version: 1
top level: luxr
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_C0062/annotation1765/range1765/1

https://synbiohub.org/public/igem/BBa_C0062/annotation1765/range1765/1
type: Range
persistent identity: range1765
display id: range1765
version: 1
direction: direction/forward
top level: luxr
start: 492
end: 492
orientation: inline

luxR
type: SequenceAnnotation
persistent identity: annotation1766
display id: annotation1766
version: 1
top level: luxr
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_C0062/annotation1766/range1766/1

https://synbiohub.org/public/igem/BBa_C0062/annotation1766/range1766/1
type: Range
persistent identity: range1766
display id: range1766
version: 1
direction: direction/forward
top level: luxr
start: 1
end: 750
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213986
display id: annotation2213986
version: 1
top level: luxr
role: SO:0000807
role: feature/barcode
location: https://synbiohub.org/public/igem/BBa_C0062/annotation2213986/range2213986/1

https://synbiohub.org/public/igem/BBa_C0062/annotation2213986/range2213986/1
type: Range
persistent identity: range2213986
display id: range2213986
version: 1
direction: direction/forward
top level: luxr
start: 757
end: 781
orientation: inline

T
type: SequenceAnnotation
persistent identity: annotation1764
display id: annotation1764
version: 1
top level: luxr
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_C0062/annotation1764/range1764/1

https://synbiohub.org/public/igem/BBa_C0062/annotation1764/range1764/1
type: Range
persistent identity: range1764
display id: range1764
version: 1
direction: direction/forward
top level: luxr
start: 174
end: 174
orientation: inline

prefix
type: SequenceAnnotation
persistent identity: annotation1762
display id: annotation1762
version: 1
top level: luxr
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_C0062/annotation1762/range1762/1

https://synbiohub.org/public/igem/BBa_C0062/annotation1762/range1762/1
type: Range
persistent identity: range1762
display id: range1762
version: 1
direction: direction/forward
top level: luxr
start: 1
end: 2
orientation: inline

BBa_C0062
type: SequenceAnnotation
persistent identity: annotation7039
display id: annotation7039
version: 1
top level: luxr
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_C0062/annotation7039/range7039/1

https://synbiohub.org/public/igem/BBa_C0062/annotation7039/range7039/1
type: Range
persistent identity: range7039
display id: range7039
version: 1
direction: direction/forward
top level: luxr
start: 1
end: 756
orientation: inline

BBa_K785004
type: ComponentDefinition
persistent identity: BBa K785004
display id: BBa_K785004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Lov-HTH (light sensor with Ptet repression) light->PtetO repressed
creator: Zining Hou
created: 2012-09-22T11:00:00Z
modified: 2015-05-08T01:13:22Z
discontinued: false
dominant: false
group u list: _1040_
m user id: 0
owner id: 11000
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This page is about a light sensor and regulator protein: Lov-HTH. Lov-HTH is a artificial light sensor designed by iGEM12 Fudan Lux team. This protein include two function domain, Lov the light sensing domain and HTH the DNA binding domain.
mutable notes: The HTH motif swap is the main issue in our design. We carefully choose the HTH sequence and swap it into the original amino acid sequence.
mutable provenance: The sequence is reverse translated from the protein EL222 http://www.rcsb.org/pdb/explore/explore.do?structureId=3P7N which is from Erythrobacter litoralis. The DNA is synthesized by Invitrogen company.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: HTH DNA binding domain
sequence annotation: Lov light sensing domain
sequence: https://synbiohub.org/public/igem/BBa_K785004_sequence/1

HTH DNA binding domain
type: SequenceAnnotation
persistent identity: annotation2194603
display id: annotation2194603
version: 1
top level: BBa_K785004
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K785004/annotation2194603/range2194603/1

https://synbiohub.org/public/igem/BBa_K785004/annotation2194603/range2194603/1
type: Range
persistent identity: range2194603
display id: range2194603
version: 1
direction: direction/forward
top level: BBa_K785004
start: 292
end: 672
orientation: inline

Lov light sensing domain
type: SequenceAnnotation
persistent identity: annotation2194602
display id: annotation2194602
version: 1
top level: BBa_K785004
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K785004/annotation2194602/range2194602/1

https://synbiohub.org/public/igem/BBa_K785004/annotation2194602/range2194602/1
type: Range
persistent identity: range2194602
display id: range2194602
version: 1
direction: direction/forward
top level: BBa_K785004
start: 1
end: 291
orientation: inline

BBa_K1031710
type: ComponentDefinition
persistent identity: BBa K1031710
display id: BBa_K1031710
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PaaX-Terminator
creator: He Shuaixin
created: 2013-09-08T11:00:00Z
modified: 2015-05-08T01:08:47Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1338_
m user id: 0
owner id: 15843
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: PaaX-TT
mutable notes: Molecular cloning
mutable provenance: PCR genome E.coli
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: PaaX
sequence annotation: BBa_B0015
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K1031710_sequence/1

PaaX
type: SequenceAnnotation
persistent identity: annotation2357486
display id: annotation2357486
version: 1
top level: BBa_K1031710
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1031710/annotation2357486/range2357486/1

https://synbiohub.org/public/igem/BBa_K1031710/annotation2357486/range2357486/1
type: Range
persistent identity: range2357486
display id: range2357486
version: 1
direction: direction/forward
top level: BBa_K1031710
start: 19
end: 969
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2357487
display id: annotation2357487
version: 1
top level: BBa_K1031710
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1031710/annotation2357487/range2357487/1

https://synbiohub.org/public/igem/BBa_K1031710/annotation2357487/range2357487/1
type: Range
persistent identity: range2357487
display id: range2357487
version: 1
direction: direction/forward
top level: BBa_K1031710
start: 978
end: 1106
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2357485
display id: annotation2357485
version: 1
top level: BBa_K1031710
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1031710/annotation2357485/range2357485/1

https://synbiohub.org/public/igem/BBa_K1031710/annotation2357485/range2357485/1
type: Range
persistent identity: range2357485
display id: range2357485
version: 1
direction: direction/forward
top level: BBa_K1031710
start: 1
end: 12
orientation: inline

BBa_K091139
type: ComponentDefinition
persistent identity: BBa K091139
display id: BBa_K091139
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS+lsrR+TT
creator: Xiao Zhu
created: 2008-07-14T11:00:00Z
modified: 2015-05-08T01:08:38Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _191_
m user id: 0
owner id: 3130
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: K091138 with double terminator B0015.
mutable notes: no
mutable provenance: lsrR comes from E. coli MG1655
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0034
component: BBa_B0010
component: BBa_K091001
component: BBa_B0012
sequence annotation: BBa_B0010
sequence annotation: BBa_B0034
sequence annotation: BBa_K091001
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_K091139_sequence/1

BBa_B0034
type: Component
persistent identity: component1967015
display id: component1967015
version: 1
top level: BBa_K091139
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0010
type: Component
persistent identity: component1967017
display id: component1967017
version: 1
top level: BBa_K091139
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_K091001
type: Component
persistent identity: component1967016
display id: component1967016
version: 1
top level: BBa_K091139
access: public
definition: https://synbiohub.org/public/igem/BBa_K091001/1

BBa_B0012
type: Component
persistent identity: component1967019
display id: component1967019
version: 1
top level: BBa_K091139
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1967017
display id: annotation1967017
version: 1
top level: BBa_K091139
location: https://synbiohub.org/public/igem/BBa_K091139/annotation1967017/range1967017/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K091139/annotation1967017/range1967017/1
type: Range
persistent identity: range1967017
display id: range1967017
version: 1
direction: direction/forward
top level: BBa_K091139
start: 981
end: 1060
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1967015
display id: annotation1967015
version: 1
top level: BBa_K091139
location: https://synbiohub.org/public/igem/BBa_K091139/annotation1967015/range1967015/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K091139/annotation1967015/range1967015/1
type: Range
persistent identity: range1967015
display id: range1967015
version: 1
direction: direction/reverse
top level: BBa_K091139
start: 1
end: 12
orientation: inline

BBa_K091001
type: SequenceAnnotation
persistent identity: annotation1967016
display id: annotation1967016
version: 1
top level: BBa_K091139
location: https://synbiohub.org/public/igem/BBa_K091139/annotation1967016/range1967016/1
component: BBa_K091001

https://synbiohub.org/public/igem/BBa_K091139/annotation1967016/range1967016/1
type: Range
persistent identity: range1967016
display id: range1967016
version: 1
direction: direction/reverse
top level: BBa_K091139
start: 19
end: 972
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1967019
display id: annotation1967019
version: 1
top level: BBa_K091139
location: https://synbiohub.org/public/igem/BBa_K091139/annotation1967019/range1967019/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K091139/annotation1967019/range1967019/1
type: Range
persistent identity: range1967019
display id: range1967019
version: 1
direction: direction/forward
top level: BBa_K091139
start: 1069
end: 1109
orientation: inline

BBa_K1520519
type: ComponentDefinition
persistent identity: BBa K1520519
display id: BBa_K1520519
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PgolTS-golS-PgolB-tetR-Ter-PtetO-rfp-Ter
creator: yue wu
created: 2014-09-22T11:00:00Z
modified: 2015-05-08T01:10:49Z
discontinued: false
dominant: false
group u list: _1902_
m user id: 0
owner id: 21318
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: PgolTS-golS-PgolB-tetR-Ter-PtetO-rfp-Ter. If there exist gold ions, rfp will not be produced.
mutable notes: None
mutable provenance: PgolTS-golS-PgolB, tetR, PtetO, rfp, Ter
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1520518
component: BBa_K1520014
sequence annotation: BBa_K1520518
sequence annotation: BBa_K1520014
sequence: https://synbiohub.org/public/igem/BBa_K1520519_sequence/1

BBa_K1520518
type: Component
persistent identity: component2385752
display id: component2385752
version: 1
top level: BBa_K1520519
access: public
definition: BBa_K1520518

BBa_K1520014
type: Component
persistent identity: component2385760
display id: component2385760
version: 1
top level: BBa_K1520519
access: public
definition: https://synbiohub.org/public/igem/BBa_K1520014/1

BBa_K1520518
type: SequenceAnnotation
persistent identity: annotation2385752
display id: annotation2385752
version: 1
top level: BBa_K1520519
location: https://synbiohub.org/public/igem/BBa_K1520519/annotation2385752/range2385752/1
component: BBa_K1520518

https://synbiohub.org/public/igem/BBa_K1520519/annotation2385752/range2385752/1
type: Range
persistent identity: range2385752
display id: range2385752
version: 1
direction: direction/reverse
top level: BBa_K1520519
start: 1
end: 1601
orientation: inline

BBa_K1520014
type: SequenceAnnotation
persistent identity: annotation2385760
display id: annotation2385760
version: 1
top level: BBa_K1520519
location: https://synbiohub.org/public/igem/BBa_K1520519/annotation2385760/range2385760/1
component: BBa_K1520014

https://synbiohub.org/public/igem/BBa_K1520519/annotation2385760/range2385760/1
type: Range
persistent identity: range2385760
display id: range2385760
version: 1
direction: direction/reverse
top level: BBa_K1520519
start: 1610
end: 2540
orientation: inline

LacIm
type: ComponentDefinition
persistent identity: BBa K092800
display id: BBa_K092800
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Coding sequence for LacI modified with a different rate of translation than LacI, RBS
creator: EPF-Lausanne Team 2008
created: 2008-10-21T11:00:00Z
modified: 2015-05-08T01:08:40Z
discontinued: false
dominant: false
experience: experience/None
group u list: _193_
m user id: 0
owner id: 2618
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable provenance: LacIm from Basu et al."A synthetic multicelluar system for programmed pattern formation", Nature. 2005 Apr 28;434(7037):1130-4
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
sequence annotation: B0034
sequence annotation: LacIm
sequence: https://synbiohub.org/public/igem/BBa_K092800_sequence/1

B0034
type: SequenceAnnotation
persistent identity: annotation1984080
display id: annotation1984080
version: 1
top level: LacIm
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K092800/annotation1984080/range1984080/1

https://synbiohub.org/public/igem/BBa_K092800/annotation1984080/range1984080/1
type: Range
persistent identity: range1984080
display id: range1984080
version: 1
direction: direction/forward
top level: LacIm
start: 1
end: 12
orientation: inline

LacIm
type: SequenceAnnotation
persistent identity: annotation1984081
display id: annotation1984081
version: 1
top level: LacIm
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K092800/annotation1984081/range1984081/1

https://synbiohub.org/public/igem/BBa_K092800/annotation1984081/range1984081/1
type: Range
persistent identity: range1984081
display id: range1984081
version: 1
direction: direction/forward
top level: LacIm
start: 21
end: 1103
orientation: inline

rhlR
type: ComponentDefinition
persistent identity: BBa C0071
display id: BBa_C0071
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: rhlR repressor/activator from P. aeruginosa PA3477 (+LVA)
creator: Debra Lin, Srini Devadas, David Gray, Ronny Krashinsky, and Chris Zheng Liu, Boopers
created: 2004-01-26T12:00:00Z
modified: 2015-08-31T04:07:23Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Transcriptional regulator, in complex with N-butyryl-HSL, RhlR binds to the Rhl promoter
mutable notes: (1/28/04 Edit Jamboree) (0) No BB sites (1) ATG (2) TAATAA (3) Blast checks out (4) No codon changes (5) Maybe chassis compatability (check by experiment) (6) Codon usage not optimal (but looks OK) (7) ssrA tag added
mutable provenance: P. aeruginosa PA3477
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: LVA
sequence annotation: Help:Barcodes
sequence annotation: rhlR
sequence: https://synbiohub.org/public/igem/BBa_C0071_sequence/1

LVA
type: SequenceAnnotation
persistent identity: annotation306523
display id: annotation306523
version: 1
top level: rhlR
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_C0071/annotation306523/range306523/1

https://synbiohub.org/public/igem/BBa_C0071/annotation306523/range306523/1
type: Range
persistent identity: range306523
display id: range306523
version: 1
direction: direction/forward
top level: rhlR
start: 724
end: 756
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213995
display id: annotation2213995
version: 1
top level: rhlR
role: SO:0000807
role: feature/barcode
location: https://synbiohub.org/public/igem/BBa_C0071/annotation2213995/range2213995/1

https://synbiohub.org/public/igem/BBa_C0071/annotation2213995/range2213995/1
type: Range
persistent identity: range2213995
display id: range2213995
version: 1
direction: direction/forward
top level: rhlR
start: 763
end: 787
orientation: inline

rhlR
type: SequenceAnnotation
persistent identity: annotation301192
display id: annotation301192
version: 1
top level: rhlR
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_C0071/annotation301192/range301192/1

https://synbiohub.org/public/igem/BBa_C0071/annotation301192/range301192/1
type: Range
persistent identity: range301192
display id: range301192
version: 1
direction: direction/forward
top level: rhlR
start: 1
end: 723
orientation: inline

BBa_K1520016
type: ComponentDefinition
persistent identity: BBa K1520016
display id: BBa_K1520016
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Pcons2-rbs-luxR-Ter-Prlux-rbs-lacI-Ter
creator: yue wu
created: 2014-09-21T11:00:00Z
modified: 2015-05-08T01:10:49Z
discontinued: false
dominant: false
group u list: _1902_
m user id: 0
owner id: 21318
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Pcons2-rbs-luxR-Ter-Prlux-rbs-lacI-Ter. It produces lacI when there is enough AHL.
mutable notes: None
mutable provenance: Pcons2, luxR, Prlux, lacI, Ter
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1520003
component: BBa_K1520004
sequence annotation: BBa_K1520004
sequence annotation: BBa_K1520003
sequence: https://synbiohub.org/public/igem/BBa_K1520016_sequence/1

BBa_K1520003
type: Component
persistent identity: component2385015
display id: component2385015
version: 1
top level: BBa_K1520016
access: public
definition: BBa_K1520003

BBa_K1520004
type: Component
persistent identity: component2385025
display id: component2385025
version: 1
top level: BBa_K1520016
access: public
definition: BBa_K1520004

BBa_K1520004
type: SequenceAnnotation
persistent identity: annotation2385025
display id: annotation2385025
version: 1
top level: BBa_K1520016
location: https://synbiohub.org/public/igem/BBa_K1520016/annotation2385025/range2385025/1
component: BBa_K1520004

https://synbiohub.org/public/igem/BBa_K1520016/annotation2385025/range2385025/1
type: Range
persistent identity: range2385025
display id: range2385025
version: 1
direction: direction/reverse
top level: BBa_K1520016
start: 988
end: 2358
orientation: inline

BBa_K1520003
type: SequenceAnnotation
persistent identity: annotation2385015
display id: annotation2385015
version: 1
top level: BBa_K1520016
location: https://synbiohub.org/public/igem/BBa_K1520016/annotation2385015/range2385015/1
component: BBa_K1520003

https://synbiohub.org/public/igem/BBa_K1520016/annotation2385015/range2385015/1
type: Range
persistent identity: range2385015
display id: range2385015
version: 1
direction: direction/reverse
top level: BBa_K1520016
start: 1
end: 979
orientation: inline

BBa_I5010
type: ComponentDefinition
persistent identity: BBa I5010
display id: BBa_I5010
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Olmecs: Inducible expression of Lambda cI repressor
creator: Olmecs 2003 IAP Team
created: 2003-11-27T12:00:00Z
modified: 2015-08-31T04:07:42Z
discontinued: false
dominant: true
experience: experience/Issues
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0011
component: BBa_B0034
component: BBa_C0051
component: BBa_R0011
component: BBa_B0012
sequence annotation: BBa_B0012
sequence annotation: BBa_R0011
sequence annotation: BBa_B0034
sequence annotation: BBa_C0051
sequence annotation: BBa_B0011
sequence: https://synbiohub.org/public/igem/BBa_I5010_sequence/1

BBa_B0011
type: Component
persistent identity: component2221096
display id: component2221096
version: 1
top level: BBa_I5010
access: public
definition: https://synbiohub.org/public/igem/BBa_B0011/1

BBa_B0034
type: Component
persistent identity: component2221087
display id: component2221087
version: 1
top level: BBa_I5010
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_C0051
type: Component
persistent identity: component2221091
display id: component2221091
version: 1
top level: BBa_I5010
access: public
definition: cI lam

BBa_R0011
type: Component
persistent identity: component2221081
display id: component2221081
version: 1
top level: BBa_I5010
access: public
definition: https://synbiohub.org/public/igem/BBa_R0011/1

BBa_B0012
type: Component
persistent identity: component2221092
display id: component2221092
version: 1
top level: BBa_I5010
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2221092
display id: annotation2221092
version: 1
top level: BBa_I5010
location: https://synbiohub.org/public/igem/BBa_I5010/annotation2221092/range2221092/1
component: BBa_B0012

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type: Range
persistent identity: range2221092
display id: range2221092
version: 1
direction: direction/forward
top level: BBa_I5010
start: 865
end: 905
orientation: inline

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation2221081
display id: annotation2221081
version: 1
top level: BBa_I5010
location: https://synbiohub.org/public/igem/BBa_I5010/annotation2221081/range2221081/1
component: BBa_R0011

https://synbiohub.org/public/igem/BBa_I5010/annotation2221081/range2221081/1
type: Range
persistent identity: range2221081
display id: range2221081
version: 1
direction: direction/forward
top level: BBa_I5010
start: 1
end: 54
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2221087
display id: annotation2221087
version: 1
top level: BBa_I5010
location: https://synbiohub.org/public/igem/BBa_I5010/annotation2221087/range2221087/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I5010/annotation2221087/range2221087/1
type: Range
persistent identity: range2221087
display id: range2221087
version: 1
direction: direction/reverse
top level: BBa_I5010
start: 64
end: 75
orientation: inline

BBa_C0051
type: SequenceAnnotation
persistent identity: annotation2221091
display id: annotation2221091
version: 1
top level: BBa_I5010
location: https://synbiohub.org/public/igem/BBa_I5010/annotation2221091/range2221091/1
component: BBa_C0051

https://synbiohub.org/public/igem/BBa_I5010/annotation2221091/range2221091/1
type: Range
persistent identity: range2221091
display id: range2221091
version: 1
direction: direction/reverse
top level: BBa_I5010
start: 82
end: 856
orientation: inline

BBa_B0011
type: SequenceAnnotation
persistent identity: annotation2221096
display id: annotation2221096
version: 1
top level: BBa_I5010
location: https://synbiohub.org/public/igem/BBa_I5010/annotation2221096/range2221096/1
component: BBa_B0011

https://synbiohub.org/public/igem/BBa_I5010/annotation2221096/range2221096/1
type: Range
persistent identity: range2221096
display id: range2221096
version: 1
direction: direction/forward
top level: BBa_I5010
start: 914
end: 959
orientation: inline

BBa_K1520018
type: ComponentDefinition
persistent identity: BBa K1520018
display id: BBa_K1520018
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Prlux-rbs-cI-Ter-PcI-rbs-lacI-Ter
creator: yue wu
created: 2014-09-21T11:00:00Z
modified: 2015-05-08T01:10:49Z
discontinued: false
dominant: false
group u list: _1902_
m user id: 0
owner id: 21318
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Plasmid2 in detector: Prlux-rbs-cI-Ter-PcI-rbs-lacI-Ter It is a low level detection plasmid. It produces rfp when AHL is enough.
mutable notes: None
mutable provenance: Prlux, cI, PcI, lacI,Ter
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1520006
component: BBa_K1520007
sequence annotation: BBa_K1520007
sequence annotation: BBa_K1520006
sequence: https://synbiohub.org/public/igem/BBa_K1520018_sequence/1

BBa_K1520006
type: Component
persistent identity: component2385063
display id: component2385063
version: 1
top level: BBa_K1520018
access: public
definition: BBa_K1520006

BBa_K1520007
type: Component
persistent identity: component2385073
display id: component2385073
version: 1
top level: BBa_K1520018
access: public
definition: BBa_K1520007

BBa_K1520007
type: SequenceAnnotation
persistent identity: annotation2385073
display id: annotation2385073
version: 1
top level: BBa_K1520018
location: https://synbiohub.org/public/igem/BBa_K1520018/annotation2385073/range2385073/1
component: BBa_K1520007

https://synbiohub.org/public/igem/BBa_K1520018/annotation2385073/range2385073/1
type: Range
persistent identity: range2385073
display id: range2385073
version: 1
direction: direction/reverse
top level: BBa_K1520018
start: 1002
end: 2366
orientation: inline

BBa_K1520006
type: SequenceAnnotation
persistent identity: annotation2385063
display id: annotation2385063
version: 1
top level: BBa_K1520018
location: https://synbiohub.org/public/igem/BBa_K1520018/annotation2385063/range2385063/1
component: BBa_K1520006

https://synbiohub.org/public/igem/BBa_K1520018/annotation2385063/range2385063/1
type: Range
persistent identity: range2385063
display id: range2385063
version: 1
direction: direction/reverse
top level: BBa_K1520018
start: 1
end: 993
orientation: inline

BBa_K545006
type: ComponentDefinition
persistent identity: BBa K545006
display id: BBa_K545006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: mag
creator: Grenoble 2011
created: 2011-09-19T11:00:00Z
modified: 2015-05-08T01:12:39Z
discontinued: false
dominant: false
experience: experience/None
group u list: _712_
m user id: 0
owner id: 8648
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: Mag is a RBS containing a binding site for the rsmA protein. Fixing this RBS, rsmA inhibits the transcription, which is recovered thanks to the rsmY iRNA, when it binds to the rsmA repressor.
mutable notes: This part was build following the standard construction protocol.
mutable provenance: Pseudomonas aeruginosa
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: mag
sequence: https://synbiohub.org/public/igem/BBa_K545006_sequence/1

mag
type: SequenceAnnotation
persistent identity: annotation2137217
display id: annotation2137217
version: 1
top level: BBa_K545006
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K545006/annotation2137217/range2137217/1

https://synbiohub.org/public/igem/BBa_K545006/annotation2137217/range2137217/1
type: Range
persistent identity: range2137217
display id: range2137217
version: 1
direction: direction/forward
top level: BBa_K545006
start: 1
end: 32
orientation: inline

BBa_K081007
type: ComponentDefinition
persistent identity: BBa K081007
display id: BBa_K081007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: cI repressor (TERM-)
creator: Lorenzo Pasotti, Paolo Magni
created: 2008-10-17T11:00:00Z
modified: 2015-05-08T01:08:35Z
discontinued: false
dominant: false
experience: experience/None
group u list: _227_
m user id: 0
owner id: 2583
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: cI protein generator without terminator (strong RBS, efficiency=0.6)
mutable notes: We used BioBrick Standard Assembly.
mutable provenance: cI: Vinay S Mahajan, Brian Chow, Peter Carr, Voichita Marinescu and Alexander D. Wissner-Gross RBS: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_C0051
component: BBa_B0030
sequence annotation: BBa_B0030
sequence annotation: BBa_C0051
sequence: https://synbiohub.org/public/igem/BBa_K081007_sequence/1

BBa_C0051
type: Component
persistent identity: component2243986
display id: component2243986
version: 1
top level: BBa_K081007
access: public
definition: cI lam

BBa_B0030
type: Component
persistent identity: component2243981
display id: component2243981
version: 1
top level: BBa_K081007
access: public
definition: https://synbiohub.org/public/igem/BBa_B0030/1

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation2243981
display id: annotation2243981
version: 1
top level: BBa_K081007
location: https://synbiohub.org/public/igem/BBa_K081007/annotation2243981/range2243981/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_K081007/annotation2243981/range2243981/1
type: Range
persistent identity: range2243981
display id: range2243981
version: 1
direction: direction/forward
top level: BBa_K081007
start: 1
end: 15
orientation: inline

BBa_C0051
type: SequenceAnnotation
persistent identity: annotation2243986
display id: annotation2243986
version: 1
top level: BBa_K081007
location: https://synbiohub.org/public/igem/BBa_K081007/annotation2243986/range2243986/1
component: BBa_C0051

https://synbiohub.org/public/igem/BBa_K081007/annotation2243986/range2243986/1
type: Range
persistent identity: range2243986
display id: range2243986
version: 1
direction: direction/reverse
top level: BBa_K081007
start: 22
end: 796
orientation: inline

BBa_I5020
type: ComponentDefinition
persistent identity: BBa I5020
display id: BBa_I5020
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Olmecs: Inducible expression of 434 cI repressor
creator: Olmecs 2003 IAP Team
created: 2003-11-27T12:00:00Z
modified: 2015-08-31T04:07:42Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0034
component: BBa_R0011
component: BBa_B0012
component: BBa_C0052
component: BBa_B0011
sequence annotation: BBa_C0052
sequence annotation: BBa_B0034
sequence annotation: BBa_R0011
sequence annotation: BBa_B0011
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_I5020_sequence/1

BBa_B0034
type: Component
persistent identity: component2221104
display id: component2221104
version: 1
top level: BBa_I5020
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_R0011
type: Component
persistent identity: component2221098
display id: component2221098
version: 1
top level: BBa_I5020
access: public
definition: https://synbiohub.org/public/igem/BBa_R0011/1

BBa_B0012
type: Component
persistent identity: component2221109
display id: component2221109
version: 1
top level: BBa_I5020
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_C0052
type: Component
persistent identity: component2221106
display id: component2221106
version: 1
top level: BBa_I5020
access: public
definition: cI 434

BBa_B0011
type: Component
persistent identity: component2221113
display id: component2221113
version: 1
top level: BBa_I5020
access: public
definition: https://synbiohub.org/public/igem/BBa_B0011/1

BBa_C0052
type: SequenceAnnotation
persistent identity: annotation2221106
display id: annotation2221106
version: 1
top level: BBa_I5020
location: https://synbiohub.org/public/igem/BBa_I5020/annotation2221106/range2221106/1
component: BBa_C0052

https://synbiohub.org/public/igem/BBa_I5020/annotation2221106/range2221106/1
type: Range
persistent identity: range2221106
display id: range2221106
version: 1
direction: direction/forward
top level: BBa_I5020
start: 82
end: 750
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2221104
display id: annotation2221104
version: 1
top level: BBa_I5020
location: https://synbiohub.org/public/igem/BBa_I5020/annotation2221104/range2221104/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I5020/annotation2221104/range2221104/1
type: Range
persistent identity: range2221104
display id: range2221104
version: 1
direction: direction/reverse
top level: BBa_I5020
start: 64
end: 75
orientation: inline

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation2221098
display id: annotation2221098
version: 1
top level: BBa_I5020
location: https://synbiohub.org/public/igem/BBa_I5020/annotation2221098/range2221098/1
component: BBa_R0011

https://synbiohub.org/public/igem/BBa_I5020/annotation2221098/range2221098/1
type: Range
persistent identity: range2221098
display id: range2221098
version: 1
direction: direction/forward
top level: BBa_I5020
start: 1
end: 54
orientation: inline

BBa_B0011
type: SequenceAnnotation
persistent identity: annotation2221113
display id: annotation2221113
version: 1
top level: BBa_I5020
location: https://synbiohub.org/public/igem/BBa_I5020/annotation2221113/range2221113/1
component: BBa_B0011

https://synbiohub.org/public/igem/BBa_I5020/annotation2221113/range2221113/1
type: Range
persistent identity: range2221113
display id: range2221113
version: 1
direction: direction/forward
top level: BBa_I5020
start: 833
end: 878
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2221109
display id: annotation2221109
version: 1
top level: BBa_I5020
location: https://synbiohub.org/public/igem/BBa_I5020/annotation2221109/range2221109/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I5020/annotation2221109/range2221109/1
type: Range
persistent identity: range2221109
display id: range2221109
version: 1
direction: direction/forward
top level: BBa_I5020
start: 784
end: 824
orientation: inline

BBa_K404117
type: ComponentDefinition
persistent identity: BBa K404117
display id: BBa_K404117
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: [AAV2]-left-ITR_pCMV_betaglobin
creator: Freiburg Bioware 2010
created: 2010-10-10T11:00:00Z
modified: 2015-05-08T01:12:23Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 6733
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: left ITR
sequence annotation: CMV promoter
sequence annotation: Beta-Globin-intron
sequence: https://synbiohub.org/public/igem/BBa_K404117_sequence/1

left ITR
type: SequenceAnnotation
persistent identity: annotation2093230
display id: annotation2093230
version: 1
top level: BBa_K404117
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404117/annotation2093230/range2093230/1

https://synbiohub.org/public/igem/BBa_K404117/annotation2093230/range2093230/1
type: Range
persistent identity: range2093230
display id: range2093230
version: 1
direction: direction/forward
top level: BBa_K404117
start: 1
end: 147
orientation: inline

CMV promoter
type: SequenceAnnotation
persistent identity: annotation2093231
display id: annotation2093231
version: 1
top level: BBa_K404117
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K404117/annotation2093231/range2093231/1

https://synbiohub.org/public/igem/BBa_K404117/annotation2093231/range2093231/1
type: Range
persistent identity: range2093231
display id: range2093231
version: 1
direction: direction/forward
top level: BBa_K404117
start: 155
end: 809
orientation: inline

Beta-Globin-intron
type: SequenceAnnotation
persistent identity: annotation2093232
display id: annotation2093232
version: 1
top level: BBa_K404117
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404117/annotation2093232/range2093232/1

https://synbiohub.org/public/igem/BBa_K404117/annotation2093232/range2093232/1
type: Range
persistent identity: range2093232
display id: range2093232
version: 1
direction: direction/forward
top level: BBa_K404117
start: 816
end: 1310
orientation: inline

BBa_K1031014
type: ComponentDefinition
persistent identity: BBa K1031014
display id: BBa_K1031014
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: NahR-Psal-B0031-RiboJ-CI
creator: He Shuaixin
created: 2013-09-08T11:00:00Z
modified: 2015-05-08T01:08:47Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1338_
m user id: 0
owner id: 15843
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: NahR Psel Ribo_J CI B0031
mutable notes: Pseudomonas putida, phiR73, E.coli
mutable provenance: lab made
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: nahR
sequence annotation: cI
sequence annotation: b0031
sequence annotation: promoter
sequence: https://synbiohub.org/public/igem/BBa_K1031014_sequence/1

nahR
type: SequenceAnnotation
persistent identity: annotation2354021
display id: annotation2354021
version: 1
top level: BBa_K1031014
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1031014/annotation2354021/range2354021/1

https://synbiohub.org/public/igem/BBa_K1031014/annotation2354021/range2354021/1
type: Range
persistent identity: range2354021
display id: range2354021
version: 1
direction: direction/reverse
top level: BBa_K1031014
start: 1
end: 903
orientation: inline

cI
type: SequenceAnnotation
persistent identity: annotation2354024
display id: annotation2354024
version: 1
top level: BBa_K1031014
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1031014/annotation2354024/range2354024/1

https://synbiohub.org/public/igem/BBa_K1031014/annotation2354024/range2354024/1
type: Range
persistent identity: range2354024
display id: range2354024
version: 1
direction: direction/forward
top level: BBa_K1031014
start: 1170
end: 1886
orientation: inline

b0031
type: SequenceAnnotation
persistent identity: annotation2354023
display id: annotation2354023
version: 1
top level: BBa_K1031014
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1031014/annotation2354023/range2354023/1

https://synbiohub.org/public/igem/BBa_K1031014/annotation2354023/range2354023/1
type: Range
persistent identity: range2354023
display id: range2354023
version: 1
direction: direction/forward
top level: BBa_K1031014
start: 1150
end: 1163
orientation: inline

promoter
type: SequenceAnnotation
persistent identity: annotation2354022
display id: annotation2354022
version: 1
top level: BBa_K1031014
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1031014/annotation2354022/range2354022/1

https://synbiohub.org/public/igem/BBa_K1031014/annotation2354022/range2354022/1
type: Range
persistent identity: range2354022
display id: range2354022
version: 1
direction: direction/forward
top level: BBa_K1031014
start: 904
end: 1050
orientation: inline

BBa_K1520021
type: ComponentDefinition
persistent identity: BBa K1520021
display id: BBa_K1520021
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Pcons2-rbs-lacI-Ter-Plac-rbs-luxI-Ter-Pcons2-rbs-luxR-Ter-Prlux-rbs-rfp-Ter
creator: yue wu
created: 2014-09-21T11:00:00Z
modified: 2015-05-08T01:10:49Z
discontinued: false
dominant: false
group u list: _1902_
m user id: 0
owner id: 21318
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Plasmid in positive feedback verification: Pcons2-rbs-lacI-Ter-Plac-rbs-luxI-Ter-Pcons2-rbs-luxR-Ter-Prlux-rbs-rfp-Ter. It is a control plasmid for positive feedback circuit. It will produce rfp when there is enough IPTG.
mutable notes: None
mutable provenance: Pcons2, lacI, Plac, luxI, Pcons2, luxR, Prlux, rfp, Ter
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1520010
component: BBa_K1520020
sequence annotation: BBa_K1520010
sequence annotation: BBa_K1520020
sequence: https://synbiohub.org/public/igem/BBa_K1520021_sequence/1

BBa_K1520010
type: Component
persistent identity: component2385150
display id: component2385150
version: 1
top level: BBa_K1520021
access: public
definition: https://synbiohub.org/public/igem/BBa_K1520010/1

BBa_K1520020
type: Component
persistent identity: component2385142
display id: component2385142
version: 1
top level: BBa_K1520021
access: public
definition: BBa_K1520020

BBa_K1520010
type: SequenceAnnotation
persistent identity: annotation2385150
display id: annotation2385150
version: 1
top level: BBa_K1520021
location: https://synbiohub.org/public/igem/BBa_K1520021/annotation2385150/range2385150/1
component: BBa_K1520010

https://synbiohub.org/public/igem/BBa_K1520021/annotation2385150/range2385150/1
type: Range
persistent identity: range2385150
display id: range2385150
version: 1
direction: direction/reverse
top level: BBa_K1520021
start: 3361
end: 4292
orientation: inline

BBa_K1520020
type: SequenceAnnotation
persistent identity: annotation2385142
display id: annotation2385142
version: 1
top level: BBa_K1520021
location: https://synbiohub.org/public/igem/BBa_K1520021/annotation2385142/range2385142/1
component: BBa_K1520020

https://synbiohub.org/public/igem/BBa_K1520021/annotation2385142/range2385142/1
type: Range
persistent identity: range2385142
display id: range2385142
version: 1
direction: direction/reverse
top level: BBa_K1520021
start: 1
end: 3352
orientation: inline

BBa_K1682004
type: ComponentDefinition
persistent identity: BBa K1682004
display id: BBa_K1682004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PkdpF[-15, T>G]
creator: iGEM15_HKUST-Rice
created: 2015-08-09T11:00:00Z
modified: 2015-09-18T09:16:31Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2100_
m user id: 8758
owner id: 21235
owning group id: 9
status: status/Planning
bookmark: false
mutable description: kdpFp[-15, T>G]
mutable notes: kdpFp[-15, T>G]
mutable provenance: kdpFp[-15, T>G]
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: phospho-KdpE binding site
sequence annotation: -10
sequence annotation: -35
sequence annotation: EcoRI Illegal site removed T->G
sequence: https://synbiohub.org/public/igem/BBa_K1682004_sequence/1

phospho-KdpE binding site
type: SequenceAnnotation
persistent identity: annotation2473581
display id: annotation2473581
version: 1
top level: BBa_K1682004
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1682004/annotation2473581/range2473581/1

https://synbiohub.org/public/igem/BBa_K1682004/annotation2473581/range2473581/1
type: Range
persistent identity: range2473581
display id: range2473581
version: 1
direction: direction/forward
top level: BBa_K1682004
start: 8
end: 24
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2473582
display id: annotation2473582
version: 1
top level: BBa_K1682004
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1682004/annotation2473582/range2473582/1

https://synbiohub.org/public/igem/BBa_K1682004/annotation2473582/range2473582/1
type: Range
persistent identity: range2473582
display id: range2473582
version: 1
direction: direction/forward
top level: BBa_K1682004
start: 69
end: 74
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2473584
display id: annotation2473584
version: 1
top level: BBa_K1682004
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1682004/annotation2473584/range2473584/1

https://synbiohub.org/public/igem/BBa_K1682004/annotation2473584/range2473584/1
type: Range
persistent identity: range2473584
display id: range2473584
version: 1
direction: direction/forward
top level: BBa_K1682004
start: 42
end: 47
orientation: inline

EcoRI Illegal site removed T->G
type: SequenceAnnotation
persistent identity: annotation2475215
display id: annotation2475215
version: 1
top level: BBa_K1682004
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K1682004/annotation2475215/range2475215/1

https://synbiohub.org/public/igem/BBa_K1682004/annotation2475215/range2475215/1
type: Range
persistent identity: range2475215
display id: range2475215
version: 1
direction: direction/forward
top level: BBa_K1682004
start: 65
end: 66
orientation: inline

BBa_K1031100
type: ComponentDefinition
persistent identity: BBa K1031100
display id: BBa_K1031100
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CapR-Terminator
creator: He Shuaixin
created: 2013-09-08T11:00:00Z
modified: 2015-05-08T01:08:47Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1338_
m user id: 0
owner id: 15843
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: CapR-TT
mutable notes: Pseudomonas putida
mutable provenance: synthesis
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: B0015
sequence annotation: CapR
sequence: https://synbiohub.org/public/igem/BBa_K1031100_sequence/1

B0015
type: SequenceAnnotation
persistent identity: annotation2361122
display id: annotation2361122
version: 1
top level: BBa_K1031100
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1031100/annotation2361122/range2361122/1

https://synbiohub.org/public/igem/BBa_K1031100/annotation2361122/range2361122/1
type: Range
persistent identity: range2361122
display id: range2361122
version: 1
direction: direction/forward
top level: BBa_K1031100
start: 1699
end: 1827
orientation: inline

CapR
type: SequenceAnnotation
persistent identity: annotation2361063
display id: annotation2361063
version: 1
top level: BBa_K1031100
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1031100/annotation2361063/range2361063/1

https://synbiohub.org/public/igem/BBa_K1031100/annotation2361063/range2361063/1
type: Range
persistent identity: range2361063
display id: range2361063
version: 1
direction: direction/forward
top level: BBa_K1031100
start: 1
end: 1692
orientation: inline

BBa_I746351
type: ComponentDefinition
persistent identity: BBa I746351
display id: BBa_I746351
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pag activator from PSP3 phage
creator: Stefan Milde
created: 2007-09-11T11:00:00Z
modified: 2015-08-31T04:08:04Z
discontinued: false
dominant: false
experience: experience/None
group u list: _116_
m user id: 0
owner id: 2122
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: The pag activator taken from PSP3 phage acts on a class of inducible promoters (parts I746360 to I746365), inducing their activity to varying degrees. The part sequence does already contain a ribosome binding site (B0034)!
mutable notes: The part does contain a RBS (B0034) already.
mutable provenance: plasmid DNA supplied by Prof. Richard Calendar, University of California.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
sequence annotation: PSP3 pag
sequence annotation: B0034
sequence: https://synbiohub.org/public/igem/BBa_I746351_sequence/1

PSP3 pag
type: SequenceAnnotation
persistent identity: annotation1943870
display id: annotation1943870
version: 1
top level: BBa_I746351
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_I746351/annotation1943870/range1943870/1

https://synbiohub.org/public/igem/BBa_I746351/annotation1943870/range1943870/1
type: Range
persistent identity: range1943870
display id: range1943870
version: 1
direction: direction/forward
top level: BBa_I746351
start: 19
end: 237
orientation: inline

PSP3 pag
type: SequenceAnnotation
persistent identity: annotation1943869
display id: annotation1943869
version: 1
top level: BBa_I746351
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_I746351/annotation1943869/range1943869/1

https://synbiohub.org/public/igem/BBa_I746351/annotation1943869/range1943869/1
type: Range
persistent identity: range1943869
display id: range1943869
version: 1
direction: direction/forward
top level: BBa_I746351
start: 19
end: 19
orientation: inline

B0034
type: SequenceAnnotation
persistent identity: annotation1943868
display id: annotation1943868
version: 1
top level: BBa_I746351
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_I746351/annotation1943868/range1943868/1

https://synbiohub.org/public/igem/BBa_I746351/annotation1943868/range1943868/1
type: Range
persistent identity: range1943868
display id: range1943868
version: 1
direction: direction/forward
top level: BBa_I746351
start: 1
end: 12
orientation: inline

BBa_I1468
type: ComponentDefinition
persistent identity: BBa I1468
display id: BBa_I1468
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CinR protein generator
creator: Barry Canton
created: 2004-08-09T11:00:00Z
modified: 2015-08-31T04:07:38Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_6_
m user id: 0
owner id: 135
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Elowitz RBS. CinR CDS (no LVA tag) and terminator. Same as I0468 but no LVA tag
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_C0177
component: BBa_B0012
component: BBa_B0034
component: BBa_B0010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0034
sequence annotation: BBa_B0012
sequence annotation: BBa_C0177
sequence: https://synbiohub.org/public/igem/BBa_I1468_sequence/1

BBa_C0177
type: Component
persistent identity: component1040160
display id: component1040160
version: 1
top level: BBa_I1468
access: public
definition: https://synbiohub.org/public/igem/BBa_C0177/1

BBa_B0012
type: Component
persistent identity: component1040175
display id: component1040175
version: 1
top level: BBa_I1468
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0034
type: Component
persistent identity: component1040158
display id: component1040158
version: 1
top level: BBa_I1468
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0010
type: Component
persistent identity: component1040165
display id: component1040165
version: 1
top level: BBa_I1468
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1040165
display id: annotation1040165
version: 1
top level: BBa_I1468
location: https://synbiohub.org/public/igem/BBa_I1468/annotation1040165/range1040165/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I1468/annotation1040165/range1040165/1
type: Range
persistent identity: range1040165
display id: range1040165
version: 1
direction: direction/forward
top level: BBa_I1468
start: 756
end: 835
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1040158
display id: annotation1040158
version: 1
top level: BBa_I1468
location: https://synbiohub.org/public/igem/BBa_I1468/annotation1040158/range1040158/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I1468/annotation1040158/range1040158/1
type: Range
persistent identity: range1040158
display id: range1040158
version: 1
direction: direction/forward
top level: BBa_I1468
start: 1
end: 12
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1040175
display id: annotation1040175
version: 1
top level: BBa_I1468
location: https://synbiohub.org/public/igem/BBa_I1468/annotation1040175/range1040175/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I1468/annotation1040175/range1040175/1
type: Range
persistent identity: range1040175
display id: range1040175
version: 1
direction: direction/forward
top level: BBa_I1468
start: 844
end: 884
orientation: inline

BBa_C0177
type: SequenceAnnotation
persistent identity: annotation1040160
display id: annotation1040160
version: 1
top level: BBa_I1468
location: https://synbiohub.org/public/igem/BBa_I1468/annotation1040160/range1040160/1
component: BBa_C0177

https://synbiohub.org/public/igem/BBa_I1468/annotation1040160/range1040160/1
type: Range
persistent identity: range1040160
display id: range1040160
version: 1
direction: direction/forward
top level: BBa_I1468
start: 19
end: 747
orientation: inline

cI HK022
type: ComponentDefinition
persistent identity: BBa C0050
display id: BBa_C0050
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: cI repressor from phage HK022 (+LVA?)
creator: Reshma Shetty
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:23Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Coding region for the HK022 bacteriophage cI protein. cI binds to the HK022 pR regulator (BBa_R0050). It represses transcription of the protein encoded by the sequence 3' to the pR region. This coding sequence does not contain a RBS.
mutable notes: References (unparsed) here: Carlson NG, Little JW. Highly cooperative DNA binding by the coliphage HK022 repressor. J Mol Biol. 1993 Apr 20;230(4):1108-30. PMID: 8487297. Mao C, Little JW. Mutations affecting cooperative DNA binding of phage HK022 CI repressor. J Mol Biol. 1998 May 29;279(1):31-48. PMID: 9636698. References (unparsed) here: Carlson NG, Little JW. Highly cooperative DNA binding by the coliphage HK022 repressor. J Mol Biol. 1993 Apr 20;230(4):1108-30. PMID: 8487297. Mao C, Little JW. Mutations affecting cooperative DNA binding of phage HK022 CI repressor. J Mol Biol. 1998 May 29;279(1):31-48. PMID: 9636698. Derived from STHK022N . Response from John Little (Arizona) regarding the start of HK022 cI From: <jlittle@email.arizona.edu> Date: Tue Jan 21, 2003 4:39:21 PM US/Eastern To: "Drew Endy" <endy@MIT.EDU> Subject: RE: hk022 cI start (naive question)? Hello Drew and Michael, I seriously doubt that the extra 27 aa are part of CI. It doesn't make sense in terms of the biology, for sure. In any case, the protein we characterized starts where Carlson and Little stated; as I recall, oR3 partially overlaps the start of cI. I have a vague memory of some possibly interesting biology, having to do with multicopy plasmids. I don't recall the findings themselves. Anyway, one possible explanation was that this extra N-terminal addition was made (e.g. from the pRE promoter, or from a message that arose from transcription around the entire plasmid), making a protein with altered functions. We never followed it up. Good luck John -- Original Message -- Date: Sun, 19 Jan 2003 15:26:26 -0500 Subject: hk022 cI start (naive question)? Cc: elowitm@rockefeller.edu To: jlittle@u.arizona.edu From: Drew Endy <endy@MIT.EDU> Hi John, I'm sitting here with Michael Elowitz and we're working through the sequence for HK022 cI. We noticed that the annotation from NC_002166 includes an "extra" 81 base pairs upstream of what we thought of as the actual start. It looks like this extra …
mutable provenance: Bacteriophage HK022.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: SsrA
sequence annotation: 2
sequence annotation: BBa_C0050
sequence annotation: Help:Barcodes
sequence annotation: cI HK022
sequence annotation: OR3 partial
sequence: https://synbiohub.org/public/igem/BBa_C0050_sequence/1

SsrA
type: SequenceAnnotation
persistent identity: annotation1736
display id: annotation1736
version: 1
top level: cI HK022
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_C0050/annotation1736/range1736/1

https://synbiohub.org/public/igem/BBa_C0050/annotation1736/range1736/1
type: Range
persistent identity: range1736
display id: range1736
version: 1
direction: direction/forward
top level: cI HK022
start: 706
end: 738
orientation: inline

2
type: SequenceAnnotation
persistent identity: annotation1734
display id: annotation1734
version: 1
top level: cI HK022
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_C0050/annotation1734/range1734/1

https://synbiohub.org/public/igem/BBa_C0050/annotation1734/range1734/1
type: Range
persistent identity: range1734
display id: range1734
version: 1
direction: direction/forward
top level: cI HK022
start: 739
end: 744
orientation: inline

BBa_C0050
type: SequenceAnnotation
persistent identity: annotation7033
display id: annotation7033
version: 1
top level: cI HK022
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_C0050/annotation7033/range7033/1

https://synbiohub.org/public/igem/BBa_C0050/annotation7033/range7033/1
type: Range
persistent identity: range7033
display id: range7033
version: 1
direction: direction/forward
top level: cI HK022
start: 1
end: 744
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213990
display id: annotation2213990
version: 1
top level: cI HK022
role: SO:0000807
role: feature/barcode
location: https://synbiohub.org/public/igem/BBa_C0050/annotation2213990/range2213990/1

https://synbiohub.org/public/igem/BBa_C0050/annotation2213990/range2213990/1
type: Range
persistent identity: range2213990
display id: range2213990
version: 1
direction: direction/forward
top level: cI HK022
start: 745
end: 769
orientation: inline

cI HK022
type: SequenceAnnotation
persistent identity: annotation1735
display id: annotation1735
version: 1
top level: cI HK022
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_C0050/annotation1735/range1735/1

https://synbiohub.org/public/igem/BBa_C0050/annotation1735/range1735/1
type: Range
persistent identity: range1735
display id: range1735
version: 1
direction: direction/forward
top level: cI HK022
start: 1
end: 744
orientation: inline

OR3 partial
type: SequenceAnnotation
persistent identity: annotation1737
display id: annotation1737
version: 1
top level: cI HK022
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_C0050/annotation1737/range1737/1

https://synbiohub.org/public/igem/BBa_C0050/annotation1737/range1737/1
type: Range
persistent identity: range1737
display id: range1737
version: 1
direction: direction/forward
top level: cI HK022
start: 1
end: 8
orientation: inline

BBa_I732923
type: ComponentDefinition
persistent identity: BBa I732923
display id: BBa_I732923
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: I732907 I732922
creator: Zhan Jian
created: 2007-10-19T11:00:00Z
modified: 2015-08-31T04:08:00Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_I732907
component: BBa_I732922
sequence annotation: BBa_I732922
sequence annotation: BBa_I732907
sequence: https://synbiohub.org/public/igem/BBa_I732923_sequence/1

BBa_I732907
type: Component
persistent identity: component2321387
display id: component2321387
version: 1
top level: BBa_I732923
access: public
definition: https://synbiohub.org/public/igem/BBa_I732907/1

BBa_I732922
type: Component
persistent identity: component2321440
display id: component2321440
version: 1
top level: BBa_I732923
access: public
definition: https://synbiohub.org/public/igem/BBa_I732922/1

BBa_I732922
type: SequenceAnnotation
persistent identity: annotation2321440
display id: annotation2321440
version: 1
top level: BBa_I732923
location: https://synbiohub.org/public/igem/BBa_I732923/annotation2321440/range2321440/1
component: BBa_I732922

https://synbiohub.org/public/igem/BBa_I732923/annotation2321440/range2321440/1
type: Range
persistent identity: range2321440
display id: range2321440
version: 1
direction: direction/reverse
top level: BBa_I732923
start: 1382
end: 5682
orientation: inline

BBa_I732907
type: SequenceAnnotation
persistent identity: annotation2321387
display id: annotation2321387
version: 1
top level: BBa_I732923
location: https://synbiohub.org/public/igem/BBa_I732923/annotation2321387/range2321387/1
component: BBa_I732907

https://synbiohub.org/public/igem/BBa_I732923/annotation2321387/range2321387/1
type: Range
persistent identity: range2321387
display id: range2321387
version: 1
direction: direction/reverse
top level: BBa_I732923
start: 1
end: 1373
orientation: inline

lasr
type: ComponentDefinition
persistent identity: BBa C0079
display id: BBa_C0079
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: lasR activator from P. aeruginosa PAO1(+LVA)
creator: Alvin Carter Powers (Fighting Darwins)
created: 2004-01-27T12:00:00Z
modified: 2015-08-31T04:07:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: coding region for lasR protein, which accepts chemical signal AI-1 (made by lasI protein)
mutable notes: Mutated 480 from g to a to remove PstI site
mutable provenance: Genbank: NC_002516 (www.ncbi.nlm.nih.gov)
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Help:Barcodes
sequence annotation: LVA
sequence annotation: lasR
sequence annotation: G
sequence: https://synbiohub.org/public/igem/BBa_C0079_sequence/1

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213999
display id: annotation2213999
version: 1
top level: lasr
role: SO:0000807
role: feature/barcode
location: https://synbiohub.org/public/igem/BBa_C0079/annotation2213999/range2213999/1

https://synbiohub.org/public/igem/BBa_C0079/annotation2213999/range2213999/1
type: Range
persistent identity: range2213999
display id: range2213999
version: 1
direction: direction/forward
top level: lasr
start: 757
end: 781
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation307948
display id: annotation307948
version: 1
top level: lasr
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_C0079/annotation307948/range307948/1

https://synbiohub.org/public/igem/BBa_C0079/annotation307948/range307948/1
type: Range
persistent identity: range307948
display id: range307948
version: 1
direction: direction/forward
top level: lasr
start: 718
end: 750
orientation: inline

lasR
type: SequenceAnnotation
persistent identity: annotation307935
display id: annotation307935
version: 1
top level: lasr
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_C0079/annotation307935/range307935/1

https://synbiohub.org/public/igem/BBa_C0079/annotation307935/range307935/1
type: Range
persistent identity: range307935
display id: range307935
version: 1
direction: direction/forward
top level: lasr
start: 1
end: 717
orientation: inline

G
type: SequenceAnnotation
persistent identity: annotation308006
display id: annotation308006
version: 1
top level: lasr
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_C0079/annotation308006/range308006/1

https://synbiohub.org/public/igem/BBa_C0079/annotation308006/range308006/1
type: Range
persistent identity: range308006
display id: range308006
version: 1
direction: direction/forward
top level: lasr
start: 480
end: 480
orientation: inline

BBa_K1031017
type: ComponentDefinition
persistent identity: BBa K1031017
display id: BBa_K1031017
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: J23114-B0034-RiboJ-φR73δ
creator: He Shuaixin
created: 2013-10-21T11:00:00Z
modified: 2015-05-08T01:08:47Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1338_
m user id: 0
owner id: 15843
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: J23114-φR73Δ
mutable notes: molecular cloning
mutable provenance: φR73
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: B0034
sequence annotation: phiR73delta
sequence annotation: J23114
sequence: https://synbiohub.org/public/igem/BBa_K1031017_sequence/1

B0034
type: SequenceAnnotation
persistent identity: annotation2370287
display id: annotation2370287
version: 1
top level: BBa_K1031017
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1031017/annotation2370287/range2370287/1

https://synbiohub.org/public/igem/BBa_K1031017/annotation2370287/range2370287/1
type: Range
persistent identity: range2370287
display id: range2370287
version: 1
direction: direction/forward
top level: BBa_K1031017
start: 118
end: 129
orientation: inline

phiR73delta
type: SequenceAnnotation
persistent identity: annotation2370288
display id: annotation2370288
version: 1
top level: BBa_K1031017
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1031017/annotation2370288/range2370288/1

https://synbiohub.org/public/igem/BBa_K1031017/annotation2370288/range2370288/1
type: Range
persistent identity: range2370288
display id: range2370288
version: 1
direction: direction/forward
top level: BBa_K1031017
start: 136
end: 381
orientation: inline

J23114
type: SequenceAnnotation
persistent identity: annotation2370286
display id: annotation2370286
version: 1
top level: BBa_K1031017
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1031017/annotation2370286/range2370286/1

https://synbiohub.org/public/igem/BBa_K1031017/annotation2370286/range2370286/1
type: Range
persistent identity: range2370286
display id: range2370286
version: 1
direction: direction/forward
top level: BBa_K1031017
start: 1
end: 35
orientation: inline

BBa_K165078
type: ComponentDefinition
persistent identity: BBa K165078
display id: BBa_K165078
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LexA activable reporter on pRS306
creator: Aaron Glieberman
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:10:57Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _267_
m user id: 0
owner id: 2510
owning group id: 58
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: -
mutable notes: -
mutable provenance: Silver lab at Harvard
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Device
role: SO:0000804
sequence: https://synbiohub.org/public/igem/BBa_K165078_sequence/1

CMVTetO2
type: ComponentDefinition
persistent identity: BBa J176017
display id: BBa_J176017
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CMVTetO2
creator: Brady Laughlin
created: 2011-09-28T11:00:00Z
modified: 2015-08-31T04:08:33Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _863_
m user id: 0
owner id: 10865
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: complete tet./ dox. inducible promoter from Invitrogen vector pcDNA/FRT/TO, T-Rex system
mutable notes: TBA
mutable provenance: TBA
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: TetO x2
sequence annotation: TATA box
sequence: https://synbiohub.org/public/igem/BBa_J176017_sequence/1

TetO x2
type: SequenceAnnotation
persistent identity: annotation2147356
display id: annotation2147356
version: 1
top level: CMVTetO2
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_J176017/annotation2147356/range2147356/1

https://synbiohub.org/public/igem/BBa_J176017/annotation2147356/range2147356/1
type: Range
persistent identity: range2147356
display id: range2147356
version: 1
direction: direction/forward
top level: CMVTetO2
start: 589
end: 628
orientation: inline

TATA box
type: SequenceAnnotation
persistent identity: annotation2147355
display id: annotation2147355
version: 1
top level: CMVTetO2
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J176017/annotation2147355/range2147355/1

https://synbiohub.org/public/igem/BBa_J176017/annotation2147355/range2147355/1
type: Range
persistent identity: range2147355
display id: range2147355
version: 1
direction: direction/forward
top level: CMVTetO2
start: 573
end: 579
orientation: inline

BBa_K165075
type: ComponentDefinition
persistent identity: BBa K165075
display id: BBa_K165075
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LexA repressible reporter on pRS305
creator: John Szymanski
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:10:57Z
discontinued: false
dominant: false
group u list: _267_
m user id: 0
owner id: 2512
owning group id: 58
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: -
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
sequence: https://synbiohub.org/public/igem/BBa_K165075_sequence/1

BBa_K921001
type: ComponentDefinition
persistent identity: BBa K921001
display id: BBa_K921001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: T7 RNAP + IPTG->PoPs (Mutant II)
creator: Eric Pederson
created: 2012-09-30T11:00:00Z
modified: 2015-05-08T01:13:46Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1186_
m user id: 0
owner id: 12713
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This is a mutant T7 promoter that includes a lacO sequence. This is repressible by the LacI protein and is induced in the presence of IPTG. The promoter sits in the 5' region of the gene of interest and initiates transcription when the cell expresses T7 RNA polymerase. Be sure you are using a cell strain that includes this gene (DE3).
mutable notes: There are three portions to the T7 promoter: the recognition site, the melting box (or TATA box) and the initation site. Mutations in these sequences caused differential behavior from the wild type.
mutable provenance: The part consists of a sequences that resemble a T7 promoter and the lacO sequence found in E. coli.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/T7
role: SO:0001207
sequence annotation: Initiation
sequence annotation: LacO
sequence annotation: T7 Promoter
sequence: https://synbiohub.org/public/igem/BBa_K921001_sequence/1

Initiation
type: SequenceAnnotation
persistent identity: annotation2205298
display id: annotation2205298
version: 1
top level: BBa_K921001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K921001/annotation2205298/range2205298/1

https://synbiohub.org/public/igem/BBa_K921001/annotation2205298/range2205298/1
type: Range
persistent identity: range2205298
display id: range2205298
version: 1
direction: direction/forward
top level: BBa_K921001
start: 18
end: 20
orientation: inline

LacO
type: SequenceAnnotation
persistent identity: annotation2205297
display id: annotation2205297
version: 1
top level: BBa_K921001
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K921001/annotation2205297/range2205297/1

https://synbiohub.org/public/igem/BBa_K921001/annotation2205297/range2205297/1
type: Range
persistent identity: range2205297
display id: range2205297
version: 1
direction: direction/forward
top level: BBa_K921001
start: 22
end: 48
orientation: inline

T7 Promoter
type: SequenceAnnotation
persistent identity: annotation2205296
display id: annotation2205296
version: 1
top level: BBa_K921001
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K921001/annotation2205296/range2205296/1

https://synbiohub.org/public/igem/BBa_K921001/annotation2205296/range2205296/1
type: Range
persistent identity: range2205296
display id: range2205296
version: 1
direction: direction/forward
top level: BBa_K921001
start: 1
end: 20
orientation: inline

BBa_K118007
type: ComponentDefinition
persistent identity: BBa K118007
display id: BBa_K118007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: rbs+appY (encoding a DNA-binding transcriptional activator)
creator: Andrew Hall
created: 2008-08-07T11:00:00Z
modified: 2015-05-08T01:09:36Z
discontinued: false
dominant: false
experience: experience/None
group u list: _192_
m user id: 0
owner id: 3282
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: This is the coding sequence of appY from Escherichia coli JM109 preceded by a synthetic ribosome binding site (rbs). It encodes a transcriptional regulator related to anaerobic energy metabolism. Overexpression of the gene has been reported to result in enhanced lycopene production by E. coli (Kang et al., 2005).
mutable notes: No special considerations
mutable provenance: Escherichia coli JM109 genomic DNA.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_K118001
component: BBa_J15001
sequence annotation: BBa_K118001
sequence annotation: BBa_J15001
sequence: https://synbiohub.org/public/igem/BBa_K118007_sequence/1

BBa_K118001
type: Component
persistent identity: component1970368
display id: component1970368
version: 1
top level: BBa_K118007
access: public
definition: https://synbiohub.org/public/igem/BBa_K118001/1

BBa_J15001
type: Component
persistent identity: component1970367
display id: component1970367
version: 1
top level: BBa_K118007
access: public
definition: https://synbiohub.org/public/igem/BBa_J15001/1

BBa_K118001
type: SequenceAnnotation
persistent identity: annotation1970368
display id: annotation1970368
version: 1
top level: BBa_K118007
location: https://synbiohub.org/public/igem/BBa_K118007/annotation1970368/range1970368/1
component: BBa_K118001

https://synbiohub.org/public/igem/BBa_K118007/annotation1970368/range1970368/1
type: Range
persistent identity: range1970368
display id: range1970368
version: 1
direction: direction/reverse
top level: BBa_K118007
start: 17
end: 769
orientation: inline

BBa_J15001
type: SequenceAnnotation
persistent identity: annotation1970367
display id: annotation1970367
version: 1
top level: BBa_K118007
location: https://synbiohub.org/public/igem/BBa_K118007/annotation1970367/range1970367/1
component: BBa_J15001

https://synbiohub.org/public/igem/BBa_K118007/annotation1970367/range1970367/1
type: Range
persistent identity: range1970367
display id: range1970367
version: 1
direction: direction/reverse
top level: BBa_K118007
start: 1
end: 10
orientation: inline

BBa_K1031112
type: ComponentDefinition
persistent identity: BBa K1031112
display id: BBa_K1031112
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: J23113-CapR-Terminator
creator: He Shuaixin
created: 2013-09-08T11:00:00Z
modified: 2015-05-08T01:08:47Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1338_
m user id: 0
owner id: 15843
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: 113-CapR-TT
mutable notes: molecular cloning
mutable provenance: synthesis Pseudomonas putida
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: CapR
sequence annotation: J23113
sequence annotation: B0015
sequence: https://synbiohub.org/public/igem/BBa_K1031112_sequence/1

CapR
type: SequenceAnnotation
persistent identity: annotation2361507
display id: annotation2361507
version: 1
top level: BBa_K1031112
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1031112/annotation2361507/range2361507/1

https://synbiohub.org/public/igem/BBa_K1031112/annotation2361507/range2361507/1
type: Range
persistent identity: range2361507
display id: range2361507
version: 1
direction: direction/forward
top level: BBa_K1031112
start: 62
end: 1753
orientation: inline

J23113
type: SequenceAnnotation
persistent identity: annotation2361448
display id: annotation2361448
version: 1
top level: BBa_K1031112
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1031112/annotation2361448/range2361448/1

https://synbiohub.org/public/igem/BBa_K1031112/annotation2361448/range2361448/1
type: Range
persistent identity: range2361448
display id: range2361448
version: 1
direction: direction/forward
top level: BBa_K1031112
start: 1
end: 35
orientation: inline

B0015
type: SequenceAnnotation
persistent identity: annotation2361508
display id: annotation2361508
version: 1
top level: BBa_K1031112
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1031112/annotation2361508/range2361508/1

https://synbiohub.org/public/igem/BBa_K1031112/annotation2361508/range2361508/1
type: Range
persistent identity: range2361508
display id: range2361508
version: 1
direction: direction/forward
top level: BBa_K1031112
start: 1760
end: 1888
orientation: inline

BBa_K165086
type: ComponentDefinition
persistent identity: BBa K165086
display id: BBa_K165086
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pTEF2 + Untagged LexA activator on pRS305
creator: John Szymanski
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:10:57Z
discontinued: false
dominant: false
group u list: _267_
m user id: 0
owner id: 2512
owning group id: 58
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: -
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_K165020
component: BBa_K165037
component: BBa_K165002
sequence annotation: BBa_K165002
sequence annotation: BBa_K165037
sequence annotation: BBa_K165020
sequence: https://synbiohub.org/public/igem/BBa_K165086_sequence/1

BBa_K165020
type: Component
persistent identity: component1997183
display id: component1997183
version: 1
top level: BBa_K165086
access: public
definition: lexA

BBa_K165037
type: Component
persistent identity: component1997181
display id: component1997181
version: 1
top level: BBa_K165086
access: public
definition: https://synbiohub.org/public/igem/BBa_K165037/1

BBa_K165002
type: Component
persistent identity: component1997182
display id: component1997182
version: 1
top level: BBa_K165086
access: public
definition: https://synbiohub.org/public/igem/BBa_K165002/1

BBa_K165002
type: SequenceAnnotation
persistent identity: annotation1997182
display id: annotation1997182
version: 1
top level: BBa_K165086
location: https://synbiohub.org/public/igem/BBa_K165086/annotation1997182/range1997182/1
component: BBa_K165002

https://synbiohub.org/public/igem/BBa_K165086/annotation1997182/range1997182/1
type: Range
persistent identity: range1997182
display id: range1997182
version: 1
direction: direction/reverse
top level: BBa_K165086
start: 412
end: 429
orientation: inline

BBa_K165037
type: SequenceAnnotation
persistent identity: annotation1997181
display id: annotation1997181
version: 1
top level: BBa_K165086
location: https://synbiohub.org/public/igem/BBa_K165086/annotation1997181/range1997181/1
component: BBa_K165037

https://synbiohub.org/public/igem/BBa_K165086/annotation1997181/range1997181/1
type: Range
persistent identity: range1997181
display id: range1997181
version: 1
direction: direction/reverse
top level: BBa_K165086
start: 1
end: 403
orientation: inline

BBa_K165020
type: SequenceAnnotation
persistent identity: annotation1997183
display id: annotation1997183
version: 1
top level: BBa_K165086
location: https://synbiohub.org/public/igem/BBa_K165086/annotation1997183/range1997183/1
component: BBa_K165020

https://synbiohub.org/public/igem/BBa_K165086/annotation1997183/range1997183/1
type: Range
persistent identity: range1997183
display id: range1997183
version: 1
direction: direction/reverse
top level: BBa_K165086
start: 438
end: 1109
orientation: inline

BBa_K1520023
type: ComponentDefinition
persistent identity: BBa K1520023
display id: BBa_K1520023
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Pcons2-rbs-lacI-Ter-Plac-rbs-luxI-Ter-Pcons2-rbs-luxR-Ter-Prlux-rbs-rfp-rbs-luxR-Ter
creator: yue wu
created: 2014-09-21T11:00:00Z
modified: 2015-05-08T01:10:49Z
discontinued: false
dominant: false
group u list: _1902_
m user id: 0
owner id: 21318
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Plasmid in positive feedback verification: Pcons2-rbs-lacI-Ter-Plac-rbs-luxI-Ter-Pcons2-rbs-luxR-Ter-Prlux-rbs-rfp-rbs-luxR-Ter. It is a positive feedback plasmid using luxR as key to feedback. Introducing IPTG will produce rfp and luxR, and luxR will work back to enhance production of rfp.
mutable notes: None
mutable provenance: Pcons2, lacI, Plac, luxI, luxR, Prlux, rfp, Ter
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1520020
component: BBa_K1520012
sequence annotation: BBa_K1520020
sequence annotation: BBa_K1520012
sequence: https://synbiohub.org/public/igem/BBa_K1520023_sequence/1

BBa_K1520020
type: Component
persistent identity: component2385213
display id: component2385213
version: 1
top level: BBa_K1520023
access: public
definition: BBa_K1520020

BBa_K1520012
type: Component
persistent identity: component2385224
display id: component2385224
version: 1
top level: BBa_K1520023
access: public
definition: BBa_K1520012

BBa_K1520020
type: SequenceAnnotation
persistent identity: annotation2385213
display id: annotation2385213
version: 1
top level: BBa_K1520023
location: https://synbiohub.org/public/igem/BBa_K1520023/annotation2385213/range2385213/1
component: BBa_K1520020

https://synbiohub.org/public/igem/BBa_K1520023/annotation2385213/range2385213/1
type: Range
persistent identity: range2385213
display id: range2385213
version: 1
direction: direction/reverse
top level: BBa_K1520023
start: 1
end: 3352
orientation: inline

BBa_K1520012
type: SequenceAnnotation
persistent identity: annotation2385224
display id: annotation2385224
version: 1
top level: BBa_K1520023
location: https://synbiohub.org/public/igem/BBa_K1520023/annotation2385224/range2385224/1
component: BBa_K1520012

https://synbiohub.org/public/igem/BBa_K1520023/annotation2385224/range2385224/1
type: Range
persistent identity: range2385224
display id: range2385224
version: 1
direction: direction/reverse
top level: BBa_K1520023
start: 3361
end: 5099
orientation: inline

BBa_K165093
type: ComponentDefinition
persistent identity: BBa K165093
display id: BBa_K165093
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Zif268-HIV bs + TEF2 + Gli1 repressor (CFPx2 tagged) on pRS304*
creator: John Szymanski
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:10:57Z
discontinued: false
dominant: false
group u list: _267_
m user id: 0
owner id: 2512
owning group id: 58
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: -
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_K165011
component: BBa_K165018
component: BBa_K165007
component: BBa_K165037
component: BBa_K165003
component: BBa_K165014
component: BBa_K165002
sequence annotation: BBa_K165014
sequence annotation: BBa_K165018
sequence annotation: BBa_K165007
sequence annotation: BBa_K165011
sequence annotation: BBa_K165037
sequence annotation: BBa_K165002
sequence annotation: BBa_K165003
sequence: https://synbiohub.org/public/igem/BBa_K165093_sequence/1

BBa_K165011
type: Component
persistent identity: component1997328
display id: component1997328
version: 1
top level: BBa_K165093
access: public
definition: https://synbiohub.org/public/igem/BBa_K165011/1

BBa_K165018
type: Component
persistent identity: component1997334
display id: component1997334
version: 1
top level: BBa_K165093
access: public
definition: https://synbiohub.org/public/igem/BBa_K165018/1

BBa_K165007
type: Component
persistent identity: component1997332
display id: component1997332
version: 1
top level: BBa_K165093
access: public
definition: https://synbiohub.org/public/igem/BBa_K165007/1

BBa_K165037
type: Component
persistent identity: component1997329
display id: component1997329
version: 1
top level: BBa_K165093
access: public
definition: https://synbiohub.org/public/igem/BBa_K165037/1

BBa_K165003
type: Component
persistent identity: component1997331
display id: component1997331
version: 1
top level: BBa_K165093
access: public
definition: https://synbiohub.org/public/igem/BBa_K165003/1

BBa_K165014
type: Component
persistent identity: component1997333
display id: component1997333
version: 1
top level: BBa_K165093
access: public
definition: https://synbiohub.org/public/igem/BBa_K165014/1

BBa_K165002
type: Component
persistent identity: component1997330
display id: component1997330
version: 1
top level: BBa_K165093
access: public
definition: https://synbiohub.org/public/igem/BBa_K165002/1

BBa_K165014
type: SequenceAnnotation
persistent identity: annotation1997333
display id: annotation1997333
version: 1
top level: BBa_K165093
location: https://synbiohub.org/public/igem/BBa_K165093/annotation1997333/range1997333/1
component: BBa_K165014

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type: Range
persistent identity: range1997333
display id: range1997333
version: 1
direction: direction/reverse
top level: BBa_K165093
start: 3844
end: 5337
orientation: inline

BBa_K165018
type: SequenceAnnotation
persistent identity: annotation1997334
display id: annotation1997334
version: 1
top level: BBa_K165093
location: https://synbiohub.org/public/igem/BBa_K165093/annotation1997334/range1997334/1
component: BBa_K165018

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type: Range
persistent identity: range1997334
display id: range1997334
version: 1
direction: direction/reverse
top level: BBa_K165093
start: 5346
end: 5598
orientation: inline

BBa_K165007
type: SequenceAnnotation
persistent identity: annotation1997332
display id: annotation1997332
version: 1
top level: BBa_K165093
location: https://synbiohub.org/public/igem/BBa_K165093/annotation1997332/range1997332/1
component: BBa_K165007

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type: Range
persistent identity: range1997332
display id: range1997332
version: 1
direction: direction/reverse
top level: BBa_K165093
start: 3311
end: 3835
orientation: inline

BBa_K165011
type: SequenceAnnotation
persistent identity: annotation1997328
display id: annotation1997328
version: 1
top level: BBa_K165093
location: https://synbiohub.org/public/igem/BBa_K165093/annotation1997328/range1997328/1
component: BBa_K165011

https://synbiohub.org/public/igem/BBa_K165093/annotation1997328/range1997328/1
type: Range
persistent identity: range1997328
display id: range1997328
version: 1
direction: direction/reverse
top level: BBa_K165093
start: 1
end: 46
orientation: inline

BBa_K165037
type: SequenceAnnotation
persistent identity: annotation1997329
display id: annotation1997329
version: 1
top level: BBa_K165093
location: https://synbiohub.org/public/igem/BBa_K165093/annotation1997329/range1997329/1
component: BBa_K165037

https://synbiohub.org/public/igem/BBa_K165093/annotation1997329/range1997329/1
type: Range
persistent identity: range1997329
display id: range1997329
version: 1
direction: direction/reverse
top level: BBa_K165093
start: 55
end: 457
orientation: inline

BBa_K165002
type: SequenceAnnotation
persistent identity: annotation1997330
display id: annotation1997330
version: 1
top level: BBa_K165093
location: https://synbiohub.org/public/igem/BBa_K165093/annotation1997330/range1997330/1
component: BBa_K165002

https://synbiohub.org/public/igem/BBa_K165093/annotation1997330/range1997330/1
type: Range
persistent identity: range1997330
display id: range1997330
version: 1
direction: direction/reverse
top level: BBa_K165093
start: 466
end: 483
orientation: inline

BBa_K165003
type: SequenceAnnotation
persistent identity: annotation1997331
display id: annotation1997331
version: 1
top level: BBa_K165093
location: https://synbiohub.org/public/igem/BBa_K165093/annotation1997331/range1997331/1
component: BBa_K165003

https://synbiohub.org/public/igem/BBa_K165093/annotation1997331/range1997331/1
type: Range
persistent identity: range1997331
display id: range1997331
version: 1
direction: direction/reverse
top level: BBa_K165093
start: 492
end: 3302
orientation: inline

BBa_K1031744
type: ComponentDefinition
persistent identity: BBa K1031744
display id: BBa_K1031744
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: J23114-PaaX
creator: He Shuaixin
created: 2013-09-08T11:00:00Z
modified: 2015-05-08T01:08:48Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1338_
m user id: 0
owner id: 15843
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: 114-PaaX-TT
mutable notes: molecular cloning
mutable provenance: PCR genome E.coli
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: BBa_B0034
sequence annotation: BBa_J23114
sequence annotation: PaaX
sequence: https://synbiohub.org/public/igem/BBa_K1031744_sequence/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2357495
display id: annotation2357495
version: 1
top level: BBa_K1031744
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1031744/annotation2357495/range2357495/1

https://synbiohub.org/public/igem/BBa_K1031744/annotation2357495/range2357495/1
type: Range
persistent identity: range2357495
display id: range2357495
version: 1
direction: direction/forward
top level: BBa_K1031744
start: 44
end: 55
orientation: inline

BBa_J23114
type: SequenceAnnotation
persistent identity: annotation2357494
display id: annotation2357494
version: 1
top level: BBa_K1031744
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1031744/annotation2357494/range2357494/1

https://synbiohub.org/public/igem/BBa_K1031744/annotation2357494/range2357494/1
type: Range
persistent identity: range2357494
display id: range2357494
version: 1
direction: direction/forward
top level: BBa_K1031744
start: 1
end: 35
orientation: inline

PaaX
type: SequenceAnnotation
persistent identity: annotation2357496
display id: annotation2357496
version: 1
top level: BBa_K1031744
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1031744/annotation2357496/range2357496/1

https://synbiohub.org/public/igem/BBa_K1031744/annotation2357496/range2357496/1
type: Range
persistent identity: range2357496
display id: range2357496
version: 1
direction: direction/forward
top level: BBa_K1031744
start: 62
end: 1012
orientation: inline

BBa_K165077
type: ComponentDefinition
persistent identity: BBa K165077
display id: BBa_K165077
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: YY1 repressible reporter
creator: Aaron Glieberman
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:10:57Z
discontinued: false
dominant: false
experience: experience/None
group u list: _267_
m user id: 0
owner id: 2510
owning group id: 58
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: -
mutable notes: -
mutable provenance: Silver lab at Harvard
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
sequence: https://synbiohub.org/public/igem/BBa_K165077_sequence/1

BBa_J37033
type: ComponentDefinition
persistent identity: BBa J37033
display id: BBa_J37033
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS + LuxR
creator: John Sy
created: 2006-08-22T11:00:00Z
modified: 2015-08-31T04:08:48Z
discontinued: false
dominant: false
experience: experience/None
group u list: _66_
m user id: 0
owner id: 1052
owning group id: 66
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: LuxR coding region with RBS
mutable notes: Designed for Imperial College iGEM 2006
mutable provenance: Ligation of B0034 + C0062
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_B0034
component: BBa_C0062
sequence annotation: BBa_C0062
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_J37033_sequence/1

BBa_B0034
type: Component
persistent identity: component2243716
display id: component2243716
version: 1
top level: BBa_J37033
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_C0062
type: Component
persistent identity: component2243719
display id: component2243719
version: 1
top level: BBa_J37033
access: public
definition: luxr

BBa_C0062
type: SequenceAnnotation
persistent identity: annotation2243719
display id: annotation2243719
version: 1
top level: BBa_J37033
location: https://synbiohub.org/public/igem/BBa_J37033/annotation2243719/range2243719/1
component: BBa_C0062

https://synbiohub.org/public/igem/BBa_J37033/annotation2243719/range2243719/1
type: Range
persistent identity: range2243719
display id: range2243719
version: 1
direction: direction/forward
top level: BBa_J37033
start: 19
end: 774
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2243716
display id: annotation2243716
version: 1
top level: BBa_J37033
location: https://synbiohub.org/public/igem/BBa_J37033/annotation2243716/range2243716/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_J37033/annotation2243716/range2243716/1
type: Range
persistent identity: range2243716
display id: range2243716
version: 1
direction: direction/reverse
top level: BBa_J37033
start: 1
end: 12
orientation: inline

BBa_K082012
type: ComponentDefinition
persistent identity: BBa K082012
display id: BBa_K082012
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: B0034-LacI
creator: Zhen Xu , Binjie Xu
created: 2008-10-27T12:00:00Z
modified: 2015-05-08T01:08:35Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _228_
m user id: 0
owner id: 1560
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: none
mutable notes: none
mutable provenance: none
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_K082004
component: BBa_B0034
sequence annotation: BBa_B0034
sequence annotation: BBa_K082004
sequence: https://synbiohub.org/public/igem/BBa_K082012_sequence/1

BBa_K082004
type: Component
persistent identity: component1993206
display id: component1993206
version: 1
top level: BBa_K082012
access: public
definition: https://synbiohub.org/public/igem/BBa_K082004/1

BBa_B0034
type: Component
persistent identity: component1993205
display id: component1993205
version: 1
top level: BBa_K082012
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1993205
display id: annotation1993205
version: 1
top level: BBa_K082012
location: https://synbiohub.org/public/igem/BBa_K082012/annotation1993205/range1993205/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K082012/annotation1993205/range1993205/1
type: Range
persistent identity: range1993205
display id: range1993205
version: 1
direction: direction/reverse
top level: BBa_K082012
start: 1
end: 12
orientation: inline

BBa_K082004
type: SequenceAnnotation
persistent identity: annotation1993206
display id: annotation1993206
version: 1
top level: BBa_K082012
location: https://synbiohub.org/public/igem/BBa_K082012/annotation1993206/range1993206/1
component: BBa_K082004

https://synbiohub.org/public/igem/BBa_K082012/annotation1993206/range1993206/1
type: Range
persistent identity: range1993206
display id: range1993206
version: 1
direction: direction/reverse
top level: BBa_K082012
start: 21
end: 1103
orientation: inline

BBa_K864202
type: ComponentDefinition
persistent identity: BBa K864202
display id: BBa_K864202
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: MarR(G95S) super-repressor
creator: Erik Lundin
created: 2012-09-24T11:00:00Z
modified: 2015-05-08T01:13:37Z
discontinued: false
dominant: false
group u list: _1124_
m user id: 0
owner id: 9827
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: marR(G95S) is a super-repressor
mutable notes: marR(G95S) carries a G>A mutation at position 284 which gives a amino acid change G95S. Has been mutated from BBa_K864200.
mutable provenance: BBa_K864200
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: G95S
sequence annotation: cds
sequence annotation: G>A
sequence: https://synbiohub.org/public/igem/BBa_K864202_sequence/1

G95S
type: SequenceAnnotation
persistent identity: annotation2197127
display id: annotation2197127
version: 1
top level: BBa_K864202
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K864202/annotation2197127/range2197127/1

https://synbiohub.org/public/igem/BBa_K864202/annotation2197127/range2197127/1
type: Range
persistent identity: range2197127
display id: range2197127
version: 1
direction: direction/forward
top level: BBa_K864202
start: 283
end: 285
orientation: inline

cds
type: SequenceAnnotation
persistent identity: annotation2197126
display id: annotation2197126
version: 1
top level: BBa_K864202
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K864202/annotation2197126/range2197126/1

https://synbiohub.org/public/igem/BBa_K864202/annotation2197126/range2197126/1
type: Range
persistent identity: range2197126
display id: range2197126
version: 1
direction: direction/forward
top level: BBa_K864202
start: 1
end: 438
orientation: inline

G>A
type: SequenceAnnotation
persistent identity: annotation2197128
display id: annotation2197128
version: 1
top level: BBa_K864202
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K864202/annotation2197128/range2197128/1

https://synbiohub.org/public/igem/BBa_K864202/annotation2197128/range2197128/1
type: Range
persistent identity: range2197128
display id: range2197128
version: 1
direction: direction/forward
top level: BBa_K864202
start: 283
end: 283
orientation: inline

tetR
type: ComponentDefinition
persistent identity: BBa C0040
display id: BBa_C0040
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: tetracycline repressor from transposon Tn10 (+LVA)
creator: June Rhee, Connie Tao, Ty Thomson, Louis Waldman.
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:23Z
discontinued: false
dominant: true
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Coding region for the TetR protein without the Ribosome Binding Site. Modified with an LVA tail for rapid degradation of the protein and faster fall time for the emission. TetR binds to the pTet regulator (BBa_R0040). aTc (anhydrotetracycline) binds to TetR and inhibits its operation.
mutable notes: References (unparsed) here: Elowitz, M. B. Transport, Assembly, and Dynamics in Systems of Interacting Proteins. Thesis, Princeton Univ., Princeton (1999). Lutz R, Bujard H., Independent and tight regulation of transcriptional units in Escherichia coli via the LacR/O, the TetR/O and AraC/I1-I2 regulatory elements. Nucleic Acids Res. 1997 Mar 15;25(6):1203-10. PMID: 9092630 References (unparsed) here: Elowitz, M. B. Transport, Assembly, and Dynamics in Systems of Interacting Proteins. Thesis, Princeton Univ., Princeton (1999). Lutz R, Bujard H., Independent and tight regulation of transcriptional units in Escherichia coli via the LacR/O, the TetR/O and AraC/I1-I2 regulatory elements. Nucleic Acids Res. 1997 Mar 15;25(6):1203-10. PMID: 9092630 BBa_C0040 TetR Protein is based on the TetR sequence from Elowitz's repressilator. It has been modified to include a rapid degradation LVA tail, and includes the BioBrick standard assembly head and tail restriction sites. The RBS has been removed. The stop codon has been changed from TAA to a double stop codon TAATAA.
mutable provenance: Elowitz, M. B. Transport, Assembly, and Dynamics in Systems of Interacting Proteins. Thesis, Princeton Univ., Princeton (1999)
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: tetR
sequence annotation: SsrA
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C0040_sequence/1

tetR
type: SequenceAnnotation
persistent identity: annotation23329
display id: annotation23329
version: 1
top level: tetR
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_C0040/annotation23329/range23329/1

https://synbiohub.org/public/igem/BBa_C0040/annotation23329/range23329/1
type: Range
persistent identity: range23329
display id: range23329
version: 1
direction: direction/forward
top level: tetR
start: 4
end: 620
orientation: inline

SsrA
type: SequenceAnnotation
persistent identity: annotation23330
display id: annotation23330
version: 1
top level: tetR
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_C0040/annotation23330/range23330/1

https://synbiohub.org/public/igem/BBa_C0040/annotation23330/range23330/1
type: Range
persistent identity: range23330
display id: range23330
version: 1
direction: direction/forward
top level: tetR
start: 621
end: 654
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213989
display id: annotation2213989
version: 1
top level: tetR
role: SO:0000807
role: feature/barcode
location: https://synbiohub.org/public/igem/BBa_C0040/annotation2213989/range2213989/1

https://synbiohub.org/public/igem/BBa_C0040/annotation2213989/range2213989/1
type: Range
persistent identity: range2213989
display id: range2213989
version: 1
direction: direction/forward
top level: tetR
start: 661
end: 685
orientation: inline

BBa_K1682012
type: ComponentDefinition
persistent identity: BBa K1682012
display id: BBa_K1682012
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PphoA promoter
creator: iGEM15_HKUST-Rice
created: 2015-09-01T11:00:00Z
modified: 2015-09-18T10:16:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2100_
m user id: 21235
owner id: 26069
owning group id: 9
status: status/Planning
bookmark: false
mutable description: PphoA promoter is a phosphate-responsive promoter. In high concentration of phosphate, it will be repressed while in low phosphate concentration, it will be activated.
mutable notes: N/A
mutable provenance: Native in E. coli
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -35
sequence annotation: -10
sequence annotation: phospho-phoB
sequence: https://synbiohub.org/public/igem/BBa_K1682012_sequence/1

-35
type: SequenceAnnotation
persistent identity: annotation2476003
display id: annotation2476003
version: 1
top level: BBa_K1682012
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1682012/annotation2476003/range2476003/1

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type: Range
persistent identity: range2476003
display id: range2476003
version: 1
direction: direction/forward
top level: BBa_K1682012
start: 28
end: 33
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2476004
display id: annotation2476004
version: 1
top level: BBa_K1682012
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1682012/annotation2476004/range2476004/1

https://synbiohub.org/public/igem/BBa_K1682012/annotation2476004/range2476004/1
type: Range
persistent identity: range2476004
display id: range2476004
version: 1
direction: direction/forward
top level: BBa_K1682012
start: 50
end: 56
orientation: inline

phospho-phoB
type: SequenceAnnotation
persistent identity: annotation2476002
display id: annotation2476002
version: 1
top level: BBa_K1682012
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1682012/annotation2476002/range2476002/1

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type: Range
persistent identity: range2476002
display id: range2476002
version: 1
direction: direction/forward
top level: BBa_K1682012
start: 21
end: 40
orientation: inline

BBa_I5000
type: ComponentDefinition
persistent identity: BBa I5000
display id: BBa_I5000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Olmecs: Inducible expression of HK022 cI repressor
creator: Olmecs 2003 IAP Team
created: 2003-11-27T12:00:00Z
modified: 2015-08-31T04:07:41Z
discontinued: false
dominant: false
experience: experience/Issues
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_C0050
component: BBa_B0011
component: BBa_B0012
component: BBa_B0034
component: BBa_R0011
sequence annotation: BBa_R0011
sequence annotation: BBa_C0050
sequence annotation: BBa_B0012
sequence annotation: BBa_B0011
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_I5000_sequence/1

BBa_C0050
type: Component
persistent identity: component2221071
display id: component2221071
version: 1
top level: BBa_I5000
access: public
definition: cI HK022

BBa_B0011
type: Component
persistent identity: component2221079
display id: component2221079
version: 1
top level: BBa_I5000
access: public
definition: https://synbiohub.org/public/igem/BBa_B0011/1

BBa_B0012
type: Component
persistent identity: component2221075
display id: component2221075
version: 1
top level: BBa_I5000
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0034
type: Component
persistent identity: component2221068
display id: component2221068
version: 1
top level: BBa_I5000
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_R0011
type: Component
persistent identity: component2221062
display id: component2221062
version: 1
top level: BBa_I5000
access: public
definition: https://synbiohub.org/public/igem/BBa_R0011/1

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation2221062
display id: annotation2221062
version: 1
top level: BBa_I5000
location: https://synbiohub.org/public/igem/BBa_I5000/annotation2221062/range2221062/1
component: BBa_R0011

https://synbiohub.org/public/igem/BBa_I5000/annotation2221062/range2221062/1
type: Range
persistent identity: range2221062
display id: range2221062
version: 1
direction: direction/forward
top level: BBa_I5000
start: 1
end: 54
orientation: inline

BBa_C0050
type: SequenceAnnotation
persistent identity: annotation2221071
display id: annotation2221071
version: 1
top level: BBa_I5000
location: https://synbiohub.org/public/igem/BBa_I5000/annotation2221071/range2221071/1
component: BBa_C0050

https://synbiohub.org/public/igem/BBa_I5000/annotation2221071/range2221071/1
type: Range
persistent identity: range2221071
display id: range2221071
version: 1
direction: direction/forward
top level: BBa_I5000
start: 82
end: 825
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2221075
display id: annotation2221075
version: 1
top level: BBa_I5000
location: https://synbiohub.org/public/igem/BBa_I5000/annotation2221075/range2221075/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I5000/annotation2221075/range2221075/1
type: Range
persistent identity: range2221075
display id: range2221075
version: 1
direction: direction/forward
top level: BBa_I5000
start: 859
end: 899
orientation: inline

BBa_B0011
type: SequenceAnnotation
persistent identity: annotation2221079
display id: annotation2221079
version: 1
top level: BBa_I5000
location: https://synbiohub.org/public/igem/BBa_I5000/annotation2221079/range2221079/1
component: BBa_B0011

https://synbiohub.org/public/igem/BBa_I5000/annotation2221079/range2221079/1
type: Range
persistent identity: range2221079
display id: range2221079
version: 1
direction: direction/forward
top level: BBa_I5000
start: 908
end: 953
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2221068
display id: annotation2221068
version: 1
top level: BBa_I5000
location: https://synbiohub.org/public/igem/BBa_I5000/annotation2221068/range2221068/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I5000/annotation2221068/range2221068/1
type: Range
persistent identity: range2221068
display id: range2221068
version: 1
direction: direction/reverse
top level: BBa_I5000
start: 64
end: 75
orientation: inline

BBa_J01101
type: ComponentDefinition
persistent identity: BBa J01101
display id: BBa_J01101
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: [pTet][StrRBS][cI][DblTerm]
creator: Golden Bear
created: 2005-12-29T12:00:00Z
modified: 2015-08-31T04:08:13Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _13_
m user id: 0
owner id: 395
owning group id: 13
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: TEST [pTet][StrRBS][cI][DblTerm]
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0010
component: BBa_C0051
component: BBa_R0040
component: BBa_B0034
component: BBa_B0012
sequence annotation: BBa_B0034
sequence annotation: BBa_R0040
sequence annotation: BBa_B0012
sequence annotation: BBa_C0051
sequence annotation: BBa_B0010
sequence: https://synbiohub.org/public/igem/BBa_J01101_sequence/1

BBa_B0010
type: Component
persistent identity: component1775746
display id: component1775746
version: 1
top level: BBa_J01101
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_C0051
type: Component
persistent identity: component1775740
display id: component1775740
version: 1
top level: BBa_J01101
access: public
definition: cI lam

BBa_R0040
type: Component
persistent identity: component1775722
display id: component1775722
version: 1
top level: BBa_J01101
access: public
definition: https://synbiohub.org/public/igem/BBa_R0040/1

BBa_B0034
type: Component
persistent identity: component1775730
display id: component1775730
version: 1
top level: BBa_J01101
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0012
type: Component
persistent identity: component1775756
display id: component1775756
version: 1
top level: BBa_J01101
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1775730
display id: annotation1775730
version: 1
top level: BBa_J01101
location: https://synbiohub.org/public/igem/BBa_J01101/annotation1775730/range1775730/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_J01101/annotation1775730/range1775730/1
type: Range
persistent identity: range1775730
display id: range1775730
version: 1
direction: direction/forward
top level: BBa_J01101
start: 63
end: 74
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1775722
display id: annotation1775722
version: 1
top level: BBa_J01101
location: https://synbiohub.org/public/igem/BBa_J01101/annotation1775722/range1775722/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_J01101/annotation1775722/range1775722/1
type: Range
persistent identity: range1775722
display id: range1775722
version: 1
direction: direction/forward
top level: BBa_J01101
start: 1
end: 54
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1775756
display id: annotation1775756
version: 1
top level: BBa_J01101
location: https://synbiohub.org/public/igem/BBa_J01101/annotation1775756/range1775756/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_J01101/annotation1775756/range1775756/1
type: Range
persistent identity: range1775756
display id: range1775756
version: 1
direction: direction/forward
top level: BBa_J01101
start: 952
end: 992
orientation: inline

BBa_C0051
type: SequenceAnnotation
persistent identity: annotation1775740
display id: annotation1775740
version: 1
top level: BBa_J01101
location: https://synbiohub.org/public/igem/BBa_J01101/annotation1775740/range1775740/1
component: BBa_C0051

https://synbiohub.org/public/igem/BBa_J01101/annotation1775740/range1775740/1
type: Range
persistent identity: range1775740
display id: range1775740
version: 1
direction: direction/forward
top level: BBa_J01101
start: 81
end: 830
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1775746
display id: annotation1775746
version: 1
top level: BBa_J01101
location: https://synbiohub.org/public/igem/BBa_J01101/annotation1775746/range1775746/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_J01101/annotation1775746/range1775746/1
type: Range
persistent identity: range1775746
display id: range1775746
version: 1
direction: direction/forward
top level: BBa_J01101
start: 864
end: 943
orientation: inline

LuxOU
type: ComponentDefinition
persistent identity: BBa K131016
display id: BBa_K131016
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LuxOU from Vibrio harveyi
creator: Thane Kubik
created: 2008-09-27T11:00:00Z
modified: 2015-05-08T01:09:47Z
discontinued: false
dominant: true
experience: experience/None
group u list: _259_
m user id: 0
owner id: 3190
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: Vibrio harveyi repressor protein LuxO and phosphorelay protein LuxU genes. LuxO and LuxU are regulators of luminescence in Vibrio harveyi.
mutable notes: LuxO and LuxU are found within the operon, therefore we have decided to keep these genes together. The natural RBS was also kept.
mutable provenance: GenBank Accession# L26221 LuxO Protein ID# AAD12736.1 LuxU Protein ID# AAD12737.2 Jeremy A. Freeman and Bonnie L. Bassler. Sequence and function of LuxU: a two-component phosphorelay protein that regulates quorum sensing in Vibrio harveyi. 1999. Journal of Bacteriology. Feb 1999, p. 899-906.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
sequence annotation: Start for LuxU
sequence annotation: Stop for LuxU
sequence annotation: Stop for LuxO
sequence annotation: LuxU
sequence annotation: Start for LuxO
sequence annotation: LuxO
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_K131016_sequence/1

Start for LuxU
type: SequenceAnnotation
persistent identity: annotation1976860
display id: annotation1976860
version: 1
top level: LuxOU
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K131016/annotation1976860/range1976860/1

https://synbiohub.org/public/igem/BBa_K131016/annotation1976860/range1976860/1
type: Range
persistent identity: range1976860
display id: range1976860
version: 1
direction: direction/forward
top level: LuxOU
start: 1389
end: 1391
orientation: inline

Stop for LuxU
type: SequenceAnnotation
persistent identity: annotation1976861
display id: annotation1976861
version: 1
top level: LuxOU
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K131016/annotation1976861/range1976861/1

https://synbiohub.org/public/igem/BBa_K131016/annotation1976861/range1976861/1
type: Range
persistent identity: range1976861
display id: range1976861
version: 1
direction: direction/forward
top level: LuxOU
start: 1731
end: 1733
orientation: inline

Stop for LuxO
type: SequenceAnnotation
persistent identity: annotation1976858
display id: annotation1976858
version: 1
top level: LuxOU
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K131016/annotation1976858/range1976858/1

https://synbiohub.org/public/igem/BBa_K131016/annotation1976858/range1976858/1
type: Range
persistent identity: range1976858
display id: range1976858
version: 1
direction: direction/forward
top level: LuxOU
start: 1390
end: 1392
orientation: inline

LuxU
type: SequenceAnnotation
persistent identity: annotation1976859
display id: annotation1976859
version: 1
top level: LuxOU
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K131016/annotation1976859/range1976859/1

https://synbiohub.org/public/igem/BBa_K131016/annotation1976859/range1976859/1
type: Range
persistent identity: range1976859
display id: range1976859
version: 1
direction: direction/forward
top level: LuxOU
start: 1389
end: 1733
orientation: inline

Start for LuxO
type: SequenceAnnotation
persistent identity: annotation1976857
display id: annotation1976857
version: 1
top level: LuxOU
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K131016/annotation1976857/range1976857/1

https://synbiohub.org/public/igem/BBa_K131016/annotation1976857/range1976857/1
type: Range
persistent identity: range1976857
display id: range1976857
version: 1
direction: direction/forward
top level: LuxOU
start: 31
end: 33
orientation: inline

LuxO
type: SequenceAnnotation
persistent identity: annotation1976856
display id: annotation1976856
version: 1
top level: LuxOU
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K131016/annotation1976856/range1976856/1

https://synbiohub.org/public/igem/BBa_K131016/annotation1976856/range1976856/1
type: Range
persistent identity: range1976856
display id: range1976856
version: 1
direction: direction/forward
top level: LuxOU
start: 31
end: 1392
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation1976855
display id: annotation1976855
version: 1
top level: LuxOU
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K131016/annotation1976855/range1976855/1

https://synbiohub.org/public/igem/BBa_K131016/annotation1976855/range1976855/1
type: Range
persistent identity: range1976855
display id: range1976855
version: 1
direction: direction/forward
top level: LuxOU
start: 5
end: 12
orientation: inline

BBa_K772004
type: ComponentDefinition
persistent identity: BBa K772004
display id: BBa_K772004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: AHL regulated PoPS generator: Las receiver device
creator: Mustafa Elitok
created: 2012-09-24T11:00:00Z
modified: 2015-05-08T01:13:15Z
discontinued: false
dominant: false
group u list: _1024_
m user id: 0
owner id: 9573
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Quorum sensing is the mechanism of communication between bacteria via synthesizing and detecting chemical compounds in the media. With these mediators, the promoters and their yield can be tuned or regulated. This part represents an alternative quorum sensing receiver device, which is able to generate a PoPS response in the presence of 6HSLO3 produced by LasI, instead of BBa_F2620. This will allow iGEMers to establish communications between different bacteria colonies and it makes simple to generate easy regulated complex systems. This part is assembled with Las promoter system to improve the current quorum sensing devices and parts which commonly base on Lux promoter system.
mutable notes: Note that pLas has no RBS afterwards, the assembling methods must be selected after this consideration.
mutable provenance: pLas and LasR sequences are gathered from Registry.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: pTetR
sequence annotation: pLas
sequence annotation: Double Terminator
sequence annotation: RBS
sequence annotation: LasR
sequence: https://synbiohub.org/public/igem/BBa_K772004_sequence/1

pTetR
type: SequenceAnnotation
persistent identity: annotation2203687
display id: annotation2203687
version: 1
top level: BBa_K772004
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K772004/annotation2203687/range2203687/1

https://synbiohub.org/public/igem/BBa_K772004/annotation2203687/range2203687/1
type: Range
persistent identity: range2203687
display id: range2203687
version: 1
direction: direction/forward
top level: BBa_K772004
start: 1
end: 54
orientation: inline

pLas
type: SequenceAnnotation
persistent identity: annotation2203691
display id: annotation2203691
version: 1
top level: BBa_K772004
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K772004/annotation2203691/range2203691/1

https://synbiohub.org/public/igem/BBa_K772004/annotation2203691/range2203691/1
type: Range
persistent identity: range2203691
display id: range2203691
version: 1
direction: direction/forward
top level: BBa_K772004
start: 955
end: 1122
orientation: inline

Double Terminator
type: SequenceAnnotation
persistent identity: annotation2203690
display id: annotation2203690
version: 1
top level: BBa_K772004
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K772004/annotation2203690/range2203690/1

https://synbiohub.org/public/igem/BBa_K772004/annotation2203690/range2203690/1
type: Range
persistent identity: range2203690
display id: range2203690
version: 1
direction: direction/forward
top level: BBa_K772004
start: 825
end: 954
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2203688
display id: annotation2203688
version: 1
top level: BBa_K772004
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K772004/annotation2203688/range2203688/1

https://synbiohub.org/public/igem/BBa_K772004/annotation2203688/range2203688/1
type: Range
persistent identity: range2203688
display id: range2203688
version: 1
direction: direction/forward
top level: BBa_K772004
start: 55
end: 75
orientation: inline

LasR
type: SequenceAnnotation
persistent identity: annotation2203689
display id: annotation2203689
version: 1
top level: BBa_K772004
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K772004/annotation2203689/range2203689/1

https://synbiohub.org/public/igem/BBa_K772004/annotation2203689/range2203689/1
type: Range
persistent identity: range2203689
display id: range2203689
version: 1
direction: direction/forward
top level: BBa_K772004
start: 76
end: 824
orientation: inline

BBa_K1480003
type: ComponentDefinition
persistent identity: BBa K1480003
display id: BBa_K1480003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TetR generator (Promoter J23109)
creator: Jesus Gilberto Rodriguez Ceja
created: 2014-10-13T11:00:00Z
modified: 2015-05-08T01:10:40Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1860_
m user id: 0
owner id: 12878
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is a TetR protein generator regulated by the constitutive promoter J23109. The protein has a LVA tag for degradation.
mutable notes: It was assembled using assembly 10.
mutable provenance: Based on parts of the registry.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_C0040
component: BBa_B0034
component: BBa_B0010
component: BBa_J23109
component: BBa_B0012
sequence annotation: BBa_C0040
sequence annotation: BBa_B0012
sequence annotation: BBa_J23109
sequence annotation: BBa_B0010
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K1480003_sequence/1

BBa_C0040
type: Component
persistent identity: component2419534
display id: component2419534
version: 1
top level: BBa_K1480003
access: public
definition: tetR

BBa_B0034
type: Component
persistent identity: component2419530
display id: component2419530
version: 1
top level: BBa_K1480003
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0010
type: Component
persistent identity: component2419535
display id: component2419535
version: 1
top level: BBa_K1480003
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_J23109
type: Component
persistent identity: component2419528
display id: component2419528
version: 1
top level: BBa_K1480003
access: public
definition: https://synbiohub.org/public/igem/BBa_J23109/1

BBa_B0012
type: Component
persistent identity: component2419537
display id: component2419537
version: 1
top level: BBa_K1480003
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_C0040
type: SequenceAnnotation
persistent identity: annotation2419534
display id: annotation2419534
version: 1
top level: BBa_K1480003
location: https://synbiohub.org/public/igem/BBa_K1480003/annotation2419534/range2419534/1
component: BBa_C0040

https://synbiohub.org/public/igem/BBa_K1480003/annotation2419534/range2419534/1
type: Range
persistent identity: range2419534
display id: range2419534
version: 1
direction: direction/reverse
top level: BBa_K1480003
start: 62
end: 746
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2419537
display id: annotation2419537
version: 1
top level: BBa_K1480003
location: https://synbiohub.org/public/igem/BBa_K1480003/annotation2419537/range2419537/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K1480003/annotation2419537/range2419537/1
type: Range
persistent identity: range2419537
display id: range2419537
version: 1
direction: direction/forward
top level: BBa_K1480003
start: 843
end: 883
orientation: inline

BBa_J23109
type: SequenceAnnotation
persistent identity: annotation2419528
display id: annotation2419528
version: 1
top level: BBa_K1480003
location: https://synbiohub.org/public/igem/BBa_K1480003/annotation2419528/range2419528/1
component: BBa_J23109

https://synbiohub.org/public/igem/BBa_K1480003/annotation2419528/range2419528/1
type: Range
persistent identity: range2419528
display id: range2419528
version: 1
direction: direction/reverse
top level: BBa_K1480003
start: 1
end: 35
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2419535
display id: annotation2419535
version: 1
top level: BBa_K1480003
location: https://synbiohub.org/public/igem/BBa_K1480003/annotation2419535/range2419535/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K1480003/annotation2419535/range2419535/1
type: Range
persistent identity: range2419535
display id: range2419535
version: 1
direction: direction/forward
top level: BBa_K1480003
start: 755
end: 834
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2419530
display id: annotation2419530
version: 1
top level: BBa_K1480003
location: https://synbiohub.org/public/igem/BBa_K1480003/annotation2419530/range2419530/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1480003/annotation2419530/range2419530/1
type: Range
persistent identity: range2419530
display id: range2419530
version: 1
direction: direction/reverse
top level: BBa_K1480003
start: 44
end: 55
orientation: inline

mnt (w)
type: ComponentDefinition
persistent identity: BBa C0073
display id: BBa_C0073
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: mnt repressor (weak) from Salmonella phage P22 (+LVA)
creator: crackdots
created: 2004-01-27T12:00:00Z
modified: 2015-08-31T04:07:23Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: -- No description --
mutable notes: dimeric repressor from enterobacteriophage p22 with dissociation constant of 3*10^-8. sequence was translated with the E.Coli K12 codon usage table, and nucleotides 95-97 was changed from AAT to AAC to avoid the cut site GAATTC (94-99). the mutation R10->K10 (ctg->aaa) decreases the binding affinity of the mnt protein to its operator by approximately 12,000 fold (Kd: 2.5e-12 to 3e-8)
mutable provenance: enterobacteriophage p22
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Arg->Lys (ctg)
sequence annotation: mnt
sequence annotation: LVA
sequence annotation: Help:Barcodes
sequence annotation: T
sequence: https://synbiohub.org/public/igem/BBa_C0073_sequence/1

Arg->Lys (ctg)
type: SequenceAnnotation
persistent identity: annotation308213
display id: annotation308213
version: 1
top level: mnt (w)
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_C0073/annotation308213/range308213/1

https://synbiohub.org/public/igem/BBa_C0073/annotation308213/range308213/1
type: Range
persistent identity: range308213
display id: range308213
version: 1
direction: direction/forward
top level: mnt (w)
start: 31
end: 33
orientation: inline

mnt
type: SequenceAnnotation
persistent identity: annotation302631
display id: annotation302631
version: 1
top level: mnt (w)
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_C0073/annotation302631/range302631/1

https://synbiohub.org/public/igem/BBa_C0073/annotation302631/range302631/1
type: Range
persistent identity: range302631
display id: range302631
version: 1
direction: direction/forward
top level: mnt (w)
start: 1
end: 249
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation306535
display id: annotation306535
version: 1
top level: mnt (w)
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_C0073/annotation306535/range306535/1

https://synbiohub.org/public/igem/BBa_C0073/annotation306535/range306535/1
type: Range
persistent identity: range306535
display id: range306535
version: 1
direction: direction/forward
top level: mnt (w)
start: 250
end: 282
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2214006
display id: annotation2214006
version: 1
top level: mnt (w)
role: SO:0000807
role: feature/barcode
location: https://synbiohub.org/public/igem/BBa_C0073/annotation2214006/range2214006/1

https://synbiohub.org/public/igem/BBa_C0073/annotation2214006/range2214006/1
type: Range
persistent identity: range2214006
display id: range2214006
version: 1
direction: direction/forward
top level: mnt (w)
start: 289
end: 313
orientation: inline

T
type: SequenceAnnotation
persistent identity: annotation308457
display id: annotation308457
version: 1
top level: mnt (w)
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_C0073/annotation308457/range308457/1

https://synbiohub.org/public/igem/BBa_C0073/annotation308457/range308457/1
type: Range
persistent identity: range308457
display id: range308457
version: 1
direction: direction/forward
top level: mnt (w)
start: 95
end: 97
orientation: inline

rsmA
type: ComponentDefinition
persistent identity: BBa K845003
display id: BBa_K845003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: rsmA gene from Pseudomonas Aeruginosa
creator: Gr??gory Hansen
created: 2012-09-25T11:00:00Z
modified: 2015-05-08T01:13:33Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1105_
m user id: 0
owner id: 12465
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: The part...
mutable notes: ...
mutable provenance: RsmA is a coding sequence from Pseudomonas aeruginosa which increases the pathogenicity by allowing type VI secretion. Taken alone, it does not code for a dangerous protein and it does not increase the risk level.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: rsmA
sequence: https://synbiohub.org/public/igem/BBa_K845003_sequence/1

rsmA
type: SequenceAnnotation
persistent identity: annotation2204302
display id: annotation2204302
version: 1
top level: rsmA
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K845003/annotation2204302/range2204302/1

https://synbiohub.org/public/igem/BBa_K845003/annotation2204302/range2204302/1
type: Range
persistent identity: range2204302
display id: range2204302
version: 1
direction: direction/forward
top level: rsmA
start: 1
end: 186
orientation: inline

BBa_K544001
type: ComponentDefinition
persistent identity: BBa K544001
display id: BBa_K544001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Tetracycline Repressor with Consitutive Promoter BBa_J23103
creator: Leung Man Hong
created: 2011-09-29T11:00:00Z
modified: 2015-05-08T01:12:39Z
discontinued: false
dominant: false
group u list: _711_
m user id: 0
owner id: 6780
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: A transcriptional regulator TetR (BBa_C0040) is driven by constitutive promoter family members (BBa-J23103, BBa-J23109, BBa-J23116, and BBa-J23106), which can be used to tune the expression level of constitutively expressed TetR.
mutable notes: The SsrA at the end of BBa_C0040 is not present in our design.
mutable provenance: The source of this part are all from the registry distributions.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: BBa_B0034
sequence annotation: BBa_J23103
sequence annotation: tet-R
sequence: https://synbiohub.org/public/igem/BBa_K544001_sequence/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2147984
display id: annotation2147984
version: 1
top level: BBa_K544001
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K544001/annotation2147984/range2147984/1

https://synbiohub.org/public/igem/BBa_K544001/annotation2147984/range2147984/1
type: Range
persistent identity: range2147984
display id: range2147984
version: 1
direction: direction/forward
top level: BBa_K544001
start: 44
end: 55
orientation: inline

BBa_J23103
type: SequenceAnnotation
persistent identity: annotation2147647
display id: annotation2147647
version: 1
top level: BBa_K544001
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K544001/annotation2147647/range2147647/1

https://synbiohub.org/public/igem/BBa_K544001/annotation2147647/range2147647/1
type: Range
persistent identity: range2147647
display id: range2147647
version: 1
direction: direction/forward
top level: BBa_K544001
start: 1
end: 35
orientation: inline

tet-R
type: SequenceAnnotation
persistent identity: annotation2147985
display id: annotation2147985
version: 1
top level: BBa_K544001
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K544001/annotation2147985/range2147985/1

https://synbiohub.org/public/igem/BBa_K544001/annotation2147985/range2147985/1
type: Range
persistent identity: range2147985
display id: range2147985
version: 1
direction: direction/forward
top level: BBa_K544001
start: 62
end: 682
orientation: inline

mnt (s)
type: ComponentDefinition
persistent identity: BBa C0072
display id: BBa_C0072
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: mnt repressor (strong) from Salmonella phage P22 (+LVA)
creator: crackdots
created: 2004-01-28T12:00:00Z
modified: 2015-08-31T04:07:23Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: -- No description --
mutable notes: wild type dimeric repressor from enterobacteriophage p22 with dissociation constant of 2.5*10^-12. sequence was translated with the E.Coli K12 codon usage table, and nucleotides 95-97 was changed from AAT to AAC to avoid the cut site GAATTC (94-99). Identification of Functionally Important Residues in the DNA Binding Region of the MNT Repressor Kendall, KL; Sauer, RT The Journal of Biological Chemistry, 264(23):13706-13710, 1989
mutable provenance: enterobacteriophage p22
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: T
sequence annotation: mnt
sequence annotation: LVA
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C0072_sequence/1

T
type: SequenceAnnotation
persistent identity: annotation308502
display id: annotation308502
version: 1
top level: mnt (s)
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_C0072/annotation308502/range308502/1

https://synbiohub.org/public/igem/BBa_C0072/annotation308502/range308502/1
type: Range
persistent identity: range308502
display id: range308502
version: 1
direction: direction/forward
top level: mnt (s)
start: 95
end: 97
orientation: inline

mnt
type: SequenceAnnotation
persistent identity: annotation308499
display id: annotation308499
version: 1
top level: mnt (s)
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_C0072/annotation308499/range308499/1

https://synbiohub.org/public/igem/BBa_C0072/annotation308499/range308499/1
type: Range
persistent identity: range308499
display id: range308499
version: 1
direction: direction/forward
top level: mnt (s)
start: 1
end: 249
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation308503
display id: annotation308503
version: 1
top level: mnt (s)
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_C0072/annotation308503/range308503/1

https://synbiohub.org/public/igem/BBa_C0072/annotation308503/range308503/1
type: Range
persistent identity: range308503
display id: range308503
version: 1
direction: direction/forward
top level: mnt (s)
start: 250
end: 282
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2214010
display id: annotation2214010
version: 1
top level: mnt (s)
role: SO:0000807
role: feature/barcode
location: https://synbiohub.org/public/igem/BBa_C0072/annotation2214010/range2214010/1

https://synbiohub.org/public/igem/BBa_C0072/annotation2214010/range2214010/1
type: Range
persistent identity: range2214010
display id: range2214010
version: 1
direction: direction/forward
top level: mnt (s)
start: 289
end: 313
orientation: inline

BBa_J33205
type: ComponentDefinition
persistent identity: BBa J33205
display id: BBa_J33205
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: hybrid lambda PR-lac promoter, repressed by lambda cI and lacI, plus lacZ
creator: Chris French
created: 2006-10-25T11:00:00Z
modified: 2015-08-31T04:08:46Z
discontinued: false
dominant: false
experience: experience/None
group u list: _63_
m user id: 0
owner id: 837
owning group id: 63
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: This part is a hybrid promoter designed to be repressed by either lambda cI or LacI. It consists of the the PRM-PR promoter region of bacteriophage lambda, including cI binding sites OR1, OR2 and OR3 as well as the -35 and -10 sites of both PRM and PR, fused via an introduced XhoI site to the LacI binding site of the lac promoter. (In this version, the lacZ' gene encoding the N-terminal 76 amino acid residues of LacZ has also been included to facilitate testing.) This approximately preserves the relationship between the -10 promoter region and and the LacI binding site. Our hope is that this promoter will be effectively repressed in the presence of either lambda cI or LacI. However, this has not been properly tested at the time of writing. We can say that good LacZ activity is observed in the presence of IPTG, indicating that the basic promoter function of lambda PR appears to be intact. Our sequencing also suggests two single nucleotide mutations within OR2 as compared to published sequence, which may be natural variants or PCR-induced mutations; in the latter case, they may or may not have some effect on cI binding. Note also that this construct includes the PRM promoter, which ought to be functional in the reverse direction.
mutable notes: This part includes the entire lambda PRM-PR region, with cI binding sites OR1, OR2 and OR3 and the -35 and -10 sequences of both promoters (PRM and PR). An XhoI site was introduced by PCR, and the DNA was joined to the region of the lac promoter beginning with the LacI binding site. The distance between the LacI binding site and the PR -10 site in the fusion is approximately the same as that between the LacI binding site and its native -10 site; thus we hope that LacI will be able to repress PR in our construct. However, this has yet to be properly tested.
mutable provenance: The lambda-derived portion (bases 4 to 78) is derived from lambda DNA by PCR using primers based on published sequence (Genbank accession J02459, gi:215104). The lac portion (bases 84 to 353) is derived from E. coli BL21 genomic DNA using primers based on published sequence (Genbank accession J01636, gi:146575).
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Other
role: SO:0000110
sequence annotation: PRM -10
sequence annotation: lacZ'
sequence annotation: LacI binding site
sequence annotation: PR -35
sequence annotation: rbs
sequence annotation: XhoI fusion site
sequence annotation: cI binding site OR1
sequence annotation: PRM -35
sequence annotation: PR -10
sequence annotation: cI binding site OR2
sequence annotation: cI binding site OR3
sequence: https://synbiohub.org/public/igem/BBa_J33205_sequence/1

PRM -10
type: SequenceAnnotation
persistent identity: annotation1905248
display id: annotation1905248
version: 1
top level: BBa_J33205
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_J33205/annotation1905248/range1905248/1

https://synbiohub.org/public/igem/BBa_J33205/annotation1905248/range1905248/1
type: Range
persistent identity: range1905248
display id: range1905248
version: 1
direction: direction/reverse
top level: BBa_J33205
start: 9
end: 14
orientation: inline

lacZ'
type: SequenceAnnotation
persistent identity: annotation1905310
display id: annotation1905310
version: 1
top level: BBa_J33205
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J33205/annotation1905310/range1905310/1

https://synbiohub.org/public/igem/BBa_J33205/annotation1905310/range1905310/1
type: Range
persistent identity: range1905310
display id: range1905310
version: 1
direction: direction/forward
top level: BBa_J33205
start: 123
end: 353
orientation: inline

LacI binding site
type: SequenceAnnotation
persistent identity: annotation1905308
display id: annotation1905308
version: 1
top level: BBa_J33205
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_J33205/annotation1905308/range1905308/1

https://synbiohub.org/public/igem/BBa_J33205/annotation1905308/range1905308/1
type: Range
persistent identity: range1905308
display id: range1905308
version: 1
direction: direction/forward
top level: BBa_J33205
start: 85
end: 105
orientation: inline

PR -35
type: SequenceAnnotation
persistent identity: annotation1905278
display id: annotation1905278
version: 1
top level: BBa_J33205
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_J33205/annotation1905278/range1905278/1

https://synbiohub.org/public/igem/BBa_J33205/annotation1905278/range1905278/1
type: Range
persistent identity: range1905278
display id: range1905278
version: 1
direction: direction/forward
top level: BBa_J33205
start: 50
end: 55
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation1905309
display id: annotation1905309
version: 1
top level: BBa_J33205
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_J33205/annotation1905309/range1905309/1

https://synbiohub.org/public/igem/BBa_J33205/annotation1905309/range1905309/1
type: Range
persistent identity: range1905309
display id: range1905309
version: 1
direction: direction/forward
top level: BBa_J33205
start: 112
end: 115
orientation: inline

XhoI fusion site
type: SequenceAnnotation
persistent identity: annotation1905291
display id: annotation1905291
version: 1
top level: BBa_J33205
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J33205/annotation1905291/range1905291/1

https://synbiohub.org/public/igem/BBa_J33205/annotation1905291/range1905291/1
type: Range
persistent identity: range1905291
display id: range1905291
version: 1
direction: direction/forward
top level: BBa_J33205
start: 79
end: 84
orientation: inline

cI binding site OR1
type: SequenceAnnotation
persistent identity: annotation1905279
display id: annotation1905279
version: 1
top level: BBa_J33205
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_J33205/annotation1905279/range1905279/1

https://synbiohub.org/public/igem/BBa_J33205/annotation1905279/range1905279/1
type: Range
persistent identity: range1905279
display id: range1905279
version: 1
direction: direction/forward
top level: BBa_J33205
start: 60
end: 76
orientation: inline

PRM -35
type: SequenceAnnotation
persistent identity: annotation1905266
display id: annotation1905266
version: 1
top level: BBa_J33205
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_J33205/annotation1905266/range1905266/1

https://synbiohub.org/public/igem/BBa_J33205/annotation1905266/range1905266/1
type: Range
persistent identity: range1905266
display id: range1905266
version: 1
direction: direction/reverse
top level: BBa_J33205
start: 32
end: 37
orientation: inline

PR -10
type: SequenceAnnotation
persistent identity: annotation1905280
display id: annotation1905280
version: 1
top level: BBa_J33205
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_J33205/annotation1905280/range1905280/1

https://synbiohub.org/public/igem/BBa_J33205/annotation1905280/range1905280/1
type: Range
persistent identity: range1905280
display id: range1905280
version: 1
direction: direction/forward
top level: BBa_J33205
start: 73
end: 78
orientation: inline

cI binding site OR2
type: SequenceAnnotation
persistent identity: annotation1905267
display id: annotation1905267
version: 1
top level: BBa_J33205
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_J33205/annotation1905267/range1905267/1

https://synbiohub.org/public/igem/BBa_J33205/annotation1905267/range1905267/1
type: Range
persistent identity: range1905267
display id: range1905267
version: 1
direction: direction/forward
top level: BBa_J33205
start: 36
end: 52
orientation: inline

cI binding site OR3
type: SequenceAnnotation
persistent identity: annotation1905249
display id: annotation1905249
version: 1
top level: BBa_J33205
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_J33205/annotation1905249/range1905249/1

https://synbiohub.org/public/igem/BBa_J33205/annotation1905249/range1905249/1
type: Range
persistent identity: range1905249
display id: range1905249
version: 1
direction: direction/forward
top level: BBa_J33205
start: 13
end: 29
orientation: inline

BBa_K1031015
type: ComponentDefinition
persistent identity: BBa K1031015
display id: BBa_K1031015
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: NahR-Psal-B0032-RiboJ-CI
creator: He Shuaixin
created: 2013-09-08T11:00:00Z
modified: 2015-05-08T01:08:47Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1338_
m user id: 0
owner id: 15843
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: NahR Psel Ribo_J CI B0032
mutable notes: Pseudomonas putida, phiR73, E.coli
mutable provenance: lab made
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: cI
sequence annotation: nahR
sequence annotation: promoter
sequence annotation: b0032
sequence: https://synbiohub.org/public/igem/BBa_K1031015_sequence/1

cI
type: SequenceAnnotation
persistent identity: annotation2354028
display id: annotation2354028
version: 1
top level: BBa_K1031015
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1031015/annotation2354028/range2354028/1

https://synbiohub.org/public/igem/BBa_K1031015/annotation2354028/range2354028/1
type: Range
persistent identity: range2354028
display id: range2354028
version: 1
direction: direction/forward
top level: BBa_K1031015
start: 1169
end: 1885
orientation: inline

nahR
type: SequenceAnnotation
persistent identity: annotation2354025
display id: annotation2354025
version: 1
top level: BBa_K1031015
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1031015/annotation2354025/range2354025/1

https://synbiohub.org/public/igem/BBa_K1031015/annotation2354025/range2354025/1
type: Range
persistent identity: range2354025
display id: range2354025
version: 1
direction: direction/reverse
top level: BBa_K1031015
start: 1
end: 903
orientation: inline

promoter
type: SequenceAnnotation
persistent identity: annotation2354026
display id: annotation2354026
version: 1
top level: BBa_K1031015
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1031015/annotation2354026/range2354026/1

https://synbiohub.org/public/igem/BBa_K1031015/annotation2354026/range2354026/1
type: Range
persistent identity: range2354026
display id: range2354026
version: 1
direction: direction/forward
top level: BBa_K1031015
start: 904
end: 1050
orientation: inline

b0032
type: SequenceAnnotation
persistent identity: annotation2354027
display id: annotation2354027
version: 1
top level: BBa_K1031015
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1031015/annotation2354027/range2354027/1

https://synbiohub.org/public/igem/BBa_K1031015/annotation2354027/range2354027/1
type: Range
persistent identity: range2354027
display id: range2354027
version: 1
direction: direction/forward
top level: BBa_K1031015
start: 1150
end: 1162
orientation: inline

BBa_K1031443
type: ComponentDefinition
persistent identity: BBa K1031443
display id: BBa_K1031443
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: J23113-HcaR
creator: He Shuaixin
created: 2013-09-08T11:00:00Z
modified: 2015-05-08T01:08:47Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1338_
m user id: 0
owner id: 15843
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: 113-HcaR-TT
mutable notes: molecular cloning
mutable provenance: PCR genome E.coli
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: HcaR
sequence annotation: BBa_B0034
sequence annotation: BBa_J23113
sequence: https://synbiohub.org/public/igem/BBa_K1031443_sequence/1

HcaR
type: SequenceAnnotation
persistent identity: annotation2357461
display id: annotation2357461
version: 1
top level: BBa_K1031443
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1031443/annotation2357461/range2357461/1

https://synbiohub.org/public/igem/BBa_K1031443/annotation2357461/range2357461/1
type: Range
persistent identity: range2357461
display id: range2357461
version: 1
direction: direction/forward
top level: BBa_K1031443
start: 62
end: 952
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2357460
display id: annotation2357460
version: 1
top level: BBa_K1031443
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1031443/annotation2357460/range2357460/1

https://synbiohub.org/public/igem/BBa_K1031443/annotation2357460/range2357460/1
type: Range
persistent identity: range2357460
display id: range2357460
version: 1
direction: direction/forward
top level: BBa_K1031443
start: 44
end: 55
orientation: inline

BBa_J23113
type: SequenceAnnotation
persistent identity: annotation2357459
display id: annotation2357459
version: 1
top level: BBa_K1031443
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1031443/annotation2357459/range2357459/1

https://synbiohub.org/public/igem/BBa_K1031443/annotation2357459/range2357459/1
type: Range
persistent identity: range2357459
display id: range2357459
version: 1
direction: direction/forward
top level: BBa_K1031443
start: 1
end: 35
orientation: inline

BBa_K1520505
type: ComponentDefinition
persistent identity: BBa K1520505
display id: BBa_K1520505
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Rbs-tetR(scarless)
creator: yue wu
created: 2014-09-21T11:00:00Z
modified: 2015-05-08T01:10:49Z
discontinued: false
dominant: false
group u list: _1902_
m user id: 0
owner id: 21318
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Rbs-tetR(scarless) It is an improvement of tetR(BBa_C0040) by adding a rbs.
mutable notes: None
mutable provenance: It is an improvement of tetR(BBa_C0040) by adding a rbs.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
sequence annotation: rbs
sequence annotation: tetR
sequence: https://synbiohub.org/public/igem/BBa_K1520505_sequence/1

rbs
type: SequenceAnnotation
persistent identity: annotation2384877
display id: annotation2384877
version: 1
top level: BBa_K1520505
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1520505/annotation2384877/range2384877/1

https://synbiohub.org/public/igem/BBa_K1520505/annotation2384877/range2384877/1
type: Range
persistent identity: range2384877
display id: range2384877
version: 1
direction: direction/forward
top level: BBa_K1520505
start: 1
end: 18
orientation: inline

tetR
type: SequenceAnnotation
persistent identity: annotation2384878
display id: annotation2384878
version: 1
top level: BBa_K1520505
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1520505/annotation2384878/range2384878/1

https://synbiohub.org/public/igem/BBa_K1520505/annotation2384878/range2384878/1
type: Range
persistent identity: range2384878
display id: range2384878
version: 1
direction: direction/forward
top level: BBa_K1520505
start: 19
end: 703
orientation: inline

BBa_J06652
type: ComponentDefinition
persistent identity: BBa J06652
display id: BBa_J06652
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Test of BBa_R0010
creator: ytwang
created: 2005-06-13T11:00:00Z
modified: 2015-08-31T04:08:18Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _20_
m user id: 0
owner id: 340
owning group id: 20
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000110
role: partType/Temporary
component: BBa_B0034
component: BBa_E0030
component: BBa_B0012
component: BBa_R0010
component: BBa_B0010
sequence annotation: BBa_R0010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0034
sequence annotation: BBa_B0012
sequence annotation: BBa_E0030
sequence: https://synbiohub.org/public/igem/BBa_J06652_sequence/1

BBa_B0034
type: Component
persistent identity: component1533980
display id: component1533980
version: 1
top level: BBa_J06652
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_E0030
type: Component
persistent identity: component1533982
display id: component1533982
version: 1
top level: BBa_J06652
access: public
definition: https://synbiohub.org/public/igem/BBa_E0030/1

BBa_B0012
type: Component
persistent identity: component1533997
display id: component1533997
version: 1
top level: BBa_J06652
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_R0010
type: Component
persistent identity: component1533970
display id: component1533970
version: 1
top level: BBa_J06652
access: public
definition: https://synbiohub.org/public/igem/BBa_R0010/1

BBa_B0010
type: Component
persistent identity: component1533987
display id: component1533987
version: 1
top level: BBa_J06652
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation1533970
display id: annotation1533970
version: 1
top level: BBa_J06652
location: https://synbiohub.org/public/igem/BBa_J06652/annotation1533970/range1533970/1
component: BBa_R0010

https://synbiohub.org/public/igem/BBa_J06652/annotation1533970/range1533970/1
type: Range
persistent identity: range1533970
display id: range1533970
version: 1
direction: direction/forward
top level: BBa_J06652
start: 1
end: 200
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1533987
display id: annotation1533987
version: 1
top level: BBa_J06652
location: https://synbiohub.org/public/igem/BBa_J06652/annotation1533987/range1533987/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_J06652/annotation1533987/range1533987/1
type: Range
persistent identity: range1533987
display id: range1533987
version: 1
direction: direction/forward
top level: BBa_J06652
start: 958
end: 1037
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1533980
display id: annotation1533980
version: 1
top level: BBa_J06652
location: https://synbiohub.org/public/igem/BBa_J06652/annotation1533980/range1533980/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_J06652/annotation1533980/range1533980/1
type: Range
persistent identity: range1533980
display id: range1533980
version: 1
direction: direction/forward
top level: BBa_J06652
start: 209
end: 220
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1533997
display id: annotation1533997
version: 1
top level: BBa_J06652
location: https://synbiohub.org/public/igem/BBa_J06652/annotation1533997/range1533997/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_J06652/annotation1533997/range1533997/1
type: Range
persistent identity: range1533997
display id: range1533997
version: 1
direction: direction/forward
top level: BBa_J06652
start: 1046
end: 1086
orientation: inline

BBa_E0030
type: SequenceAnnotation
persistent identity: annotation1533982
display id: annotation1533982
version: 1
top level: BBa_J06652
location: https://synbiohub.org/public/igem/BBa_J06652/annotation1533982/range1533982/1
component: BBa_E0030

https://synbiohub.org/public/igem/BBa_J06652/annotation1533982/range1533982/1
type: Range
persistent identity: range1533982
display id: range1533982
version: 1
direction: direction/forward
top level: BBa_J06652
start: 227
end: 949
orientation: inline

BBa_K094101
type: ComponentDefinition
persistent identity: BBa K094101
display id: BBa_K094101
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: plambda P(O-R12)-rbs-cheZ
creator: Shi Lei
created: 2008-10-12T11:00:00Z
modified: 2015-05-08T01:08:40Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _255_
m user id: 0
owner id: 2589
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part contains a lambda promoter that is constitutively on and can be repressed by CI protein. cheZ protein is responsible for the dephosphorylation of cheY protein in bacteria flagella movement. This part lacks a terminator.
mutable notes: the terminator is not included in this part
mutable provenance: From non-biobrick plasmid, flanked by biobrick prefix and surfix.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_B0034
component: BBa_K094002
component: BBa_K094100
sequence annotation: BBa_B0034
sequence annotation: BBa_K094100
sequence annotation: BBa_K094002
sequence: https://synbiohub.org/public/igem/BBa_K094101_sequence/1

BBa_B0034
type: Component
persistent identity: component1990802
display id: component1990802
version: 1
top level: BBa_K094101
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K094002
type: Component
persistent identity: component1990800
display id: component1990800
version: 1
top level: BBa_K094101
access: public
definition: https://synbiohub.org/public/igem/BBa_K094002/1

BBa_K094100
type: Component
persistent identity: component1990804
display id: component1990804
version: 1
top level: BBa_K094101
access: public
definition: https://synbiohub.org/public/igem/BBa_K094100/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1990802
display id: annotation1990802
version: 1
top level: BBa_K094101
location: https://synbiohub.org/public/igem/BBa_K094101/annotation1990802/range1990802/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K094101/annotation1990802/range1990802/1
type: Range
persistent identity: range1990802
display id: range1990802
version: 1
direction: direction/reverse
top level: BBa_K094101
start: 109
end: 120
orientation: inline

BBa_K094100
type: SequenceAnnotation
persistent identity: annotation1990804
display id: annotation1990804
version: 1
top level: BBa_K094101
location: https://synbiohub.org/public/igem/BBa_K094101/annotation1990804/range1990804/1
component: BBa_K094100

https://synbiohub.org/public/igem/BBa_K094101/annotation1990804/range1990804/1
type: Range
persistent identity: range1990804
display id: range1990804
version: 1
direction: direction/reverse
top level: BBa_K094101
start: 127
end: 821
orientation: inline

BBa_K094002
type: SequenceAnnotation
persistent identity: annotation1990800
display id: annotation1990800
version: 1
top level: BBa_K094101
location: https://synbiohub.org/public/igem/BBa_K094101/annotation1990800/range1990800/1
component: BBa_K094002

https://synbiohub.org/public/igem/BBa_K094101/annotation1990800/range1990800/1
type: Range
persistent identity: range1990800
display id: range1990800
version: 1
direction: direction/reverse
top level: BBa_K094101
start: 1
end: 100
orientation: inline

BBa_K1031913
type: ComponentDefinition
persistent identity: BBa K1031913
display id: BBa_K1031913
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: J23109-XylS-Terminator
creator: He Shuaixin
created: 2013-09-08T11:00:00Z
modified: 2015-05-08T01:08:48Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1338_
m user id: 0
owner id: 15843
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: 109-XylS-TT
mutable notes: molecular cloning
mutable provenance: synthesis Pseudomonas
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: B0015
sequence annotation: J23109
sequence annotation: XylS
sequence: https://synbiohub.org/public/igem/BBa_K1031913_sequence/1

B0015
type: SequenceAnnotation
persistent identity: annotation2360171
display id: annotation2360171
version: 1
top level: BBa_K1031913
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1031913/annotation2360171/range2360171/1

https://synbiohub.org/public/igem/BBa_K1031913/annotation2360171/range2360171/1
type: Range
persistent identity: range2360171
display id: range2360171
version: 1
direction: direction/forward
top level: BBa_K1031913
start: 1034
end: 1162
orientation: inline

J23109
type: SequenceAnnotation
persistent identity: annotation2360169
display id: annotation2360169
version: 1
top level: BBa_K1031913
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1031913/annotation2360169/range2360169/1

https://synbiohub.org/public/igem/BBa_K1031913/annotation2360169/range2360169/1
type: Range
persistent identity: range2360169
display id: range2360169
version: 1
direction: direction/forward
top level: BBa_K1031913
start: 1
end: 35
orientation: inline

XylS
type: SequenceAnnotation
persistent identity: annotation2360170
display id: annotation2360170
version: 1
top level: BBa_K1031913
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1031913/annotation2360170/range2360170/1

https://synbiohub.org/public/igem/BBa_K1031913/annotation2360170/range2360170/1
type: Range
persistent identity: range2360170
display id: range2360170
version: 1
direction: direction/forward
top level: BBa_K1031913
start: 36
end: 1027
orientation: inline

BBa_I5030
type: ComponentDefinition
persistent identity: BBa I5030
display id: BBa_I5030
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Olmecs: Inducible expression of P22 c2 repressor
creator: Olmecs 2003 IAP Team
created: 2003-11-27T12:00:00Z
modified: 2015-08-31T04:07:42Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0034
component: BBa_R0011
component: BBa_C0053
component: BBa_B0011
component: BBa_B0012
sequence annotation: BBa_B0012
sequence annotation: BBa_B0011
sequence annotation: BBa_B0034
sequence annotation: BBa_R0011
sequence annotation: BBa_C0053
sequence: https://synbiohub.org/public/igem/BBa_I5030_sequence/1

BBa_B0034
type: Component
persistent identity: component2221121
display id: component2221121
version: 1
top level: BBa_I5030
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_R0011
type: Component
persistent identity: component2221115
display id: component2221115
version: 1
top level: BBa_I5030
access: public
definition: https://synbiohub.org/public/igem/BBa_R0011/1

BBa_C0053
type: Component
persistent identity: component2221123
display id: component2221123
version: 1
top level: BBa_I5030
access: public
definition: c2 P22

BBa_B0011
type: Component
persistent identity: component2221131
display id: component2221131
version: 1
top level: BBa_I5030
access: public
definition: https://synbiohub.org/public/igem/BBa_B0011/1

BBa_B0012
type: Component
persistent identity: component2221127
display id: component2221127
version: 1
top level: BBa_I5030
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2221127
display id: annotation2221127
version: 1
top level: BBa_I5030
location: https://synbiohub.org/public/igem/BBa_I5030/annotation2221127/range2221127/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I5030/annotation2221127/range2221127/1
type: Range
persistent identity: range2221127
display id: range2221127
version: 1
direction: direction/forward
top level: BBa_I5030
start: 802
end: 842
orientation: inline

BBa_B0011
type: SequenceAnnotation
persistent identity: annotation2221131
display id: annotation2221131
version: 1
top level: BBa_I5030
location: https://synbiohub.org/public/igem/BBa_I5030/annotation2221131/range2221131/1
component: BBa_B0011

https://synbiohub.org/public/igem/BBa_I5030/annotation2221131/range2221131/1
type: Range
persistent identity: range2221131
display id: range2221131
version: 1
direction: direction/forward
top level: BBa_I5030
start: 851
end: 896
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2221121
display id: annotation2221121
version: 1
top level: BBa_I5030
location: https://synbiohub.org/public/igem/BBa_I5030/annotation2221121/range2221121/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I5030/annotation2221121/range2221121/1
type: Range
persistent identity: range2221121
display id: range2221121
version: 1
direction: direction/reverse
top level: BBa_I5030
start: 64
end: 75
orientation: inline

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation2221115
display id: annotation2221115
version: 1
top level: BBa_I5030
location: https://synbiohub.org/public/igem/BBa_I5030/annotation2221115/range2221115/1
component: BBa_R0011

https://synbiohub.org/public/igem/BBa_I5030/annotation2221115/range2221115/1
type: Range
persistent identity: range2221115
display id: range2221115
version: 1
direction: direction/forward
top level: BBa_I5030
start: 1
end: 54
orientation: inline

BBa_C0053
type: SequenceAnnotation
persistent identity: annotation2221123
display id: annotation2221123
version: 1
top level: BBa_I5030
location: https://synbiohub.org/public/igem/BBa_I5030/annotation2221123/range2221123/1
component: BBa_C0053

https://synbiohub.org/public/igem/BBa_I5030/annotation2221123/range2221123/1
type: Range
persistent identity: range2221123
display id: range2221123
version: 1
direction: direction/forward
top level: BBa_I5030
start: 82
end: 768
orientation: inline

BBa_K165089
type: ComponentDefinition
persistent identity: BBa K165089
display id: BBa_K165089
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Gli1 bs + TEF2 + Untagged LexA activator on pRS306
creator: John Szymanski
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:10:57Z
discontinued: false
dominant: false
group u list: _267_
m user id: 0
owner id: 2512
owning group id: 58
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: -
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_K165012
component: BBa_K165020
component: BBa_K165037
sequence annotation: BBa_K165037
sequence annotation: BBa_K165012
sequence annotation: BBa_K165020
sequence: https://synbiohub.org/public/igem/BBa_K165089_sequence/1

BBa_K165012
type: Component
persistent identity: component1997206
display id: component1997206
version: 1
top level: BBa_K165089
access: public
definition: https://synbiohub.org/public/igem/BBa_K165012/1

BBa_K165020
type: Component
persistent identity: component1997208
display id: component1997208
version: 1
top level: BBa_K165089
access: public
definition: lexA

BBa_K165037
type: Component
persistent identity: component1997207
display id: component1997207
version: 1
top level: BBa_K165089
access: public
definition: https://synbiohub.org/public/igem/BBa_K165037/1

BBa_K165037
type: SequenceAnnotation
persistent identity: annotation1997207
display id: annotation1997207
version: 1
top level: BBa_K165089
location: https://synbiohub.org/public/igem/BBa_K165089/annotation1997207/range1997207/1
component: BBa_K165037

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type: Range
persistent identity: range1997207
display id: range1997207
version: 1
direction: direction/reverse
top level: BBa_K165089
start: 136
end: 538
orientation: inline

BBa_K165012
type: SequenceAnnotation
persistent identity: annotation1997206
display id: annotation1997206
version: 1
top level: BBa_K165089
location: https://synbiohub.org/public/igem/BBa_K165089/annotation1997206/range1997206/1
component: BBa_K165012

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type: Range
persistent identity: range1997206
display id: range1997206
version: 1
direction: direction/reverse
top level: BBa_K165089
start: 1
end: 127
orientation: inline

BBa_K165020
type: SequenceAnnotation
persistent identity: annotation1997208
display id: annotation1997208
version: 1
top level: BBa_K165089
location: https://synbiohub.org/public/igem/BBa_K165089/annotation1997208/range1997208/1
component: BBa_K165020

https://synbiohub.org/public/igem/BBa_K165089/annotation1997208/range1997208/1
type: Range
persistent identity: range1997208
display id: range1997208
version: 1
direction: direction/reverse
top level: BBa_K165089
start: 547
end: 1218
orientation: inline

BBa_K1520007
type: ComponentDefinition
persistent identity: BBa K1520007
display id: BBa_K1520007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PcI-rbs-lacI-Ter
creator: yue wu
created: 2014-09-21T11:00:00Z
modified: 2015-05-08T01:10:48Z
discontinued: false
dominant: false
group u list: _1902_
m user id: 0
owner id: 21318
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: PcI-rbs-lacI-Ter. It produces enough lacI when there is less cI.
mutable notes: None
mutable provenance: PcI, lacI, Ter
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1520502
component: BBa_K1520029
component: BBa_R0051
sequence annotation: BBa_K1520029
sequence annotation: BBa_K1520502
sequence annotation: BBa_R0051
sequence: https://synbiohub.org/public/igem/BBa_K1520007_sequence/1

BBa_K1520502
type: Component
persistent identity: component2384930
display id: component2384930
version: 1
top level: BBa_K1520007
access: public
definition: BBa_K1520502

BBa_K1520029
type: Component
persistent identity: component2384931
display id: component2384931
version: 1
top level: BBa_K1520007
access: public
definition: https://synbiohub.org/public/igem/BBa_K1520029/1

BBa_R0051
type: Component
persistent identity: component2384924
display id: component2384924
version: 1
top level: BBa_K1520007
access: public
definition: https://synbiohub.org/public/igem/BBa_R0051/1

BBa_K1520029
type: SequenceAnnotation
persistent identity: annotation2384931
display id: annotation2384931
version: 1
top level: BBa_K1520007
location: https://synbiohub.org/public/igem/BBa_K1520007/annotation2384931/range2384931/1
component: BBa_K1520029

https://synbiohub.org/public/igem/BBa_K1520007/annotation2384931/range2384931/1
type: Range
persistent identity: range2384931
display id: range2384931
version: 1
direction: direction/reverse
top level: BBa_K1520007
start: 1237
end: 1365
orientation: inline

BBa_K1520502
type: SequenceAnnotation
persistent identity: annotation2384930
display id: annotation2384930
version: 1
top level: BBa_K1520007
location: https://synbiohub.org/public/igem/BBa_K1520007/annotation2384930/range2384930/1
component: BBa_K1520502

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type: Range
persistent identity: range2384930
display id: range2384930
version: 1
direction: direction/reverse
top level: BBa_K1520007
start: 58
end: 1228
orientation: inline

BBa_R0051
type: SequenceAnnotation
persistent identity: annotation2384924
display id: annotation2384924
version: 1
top level: BBa_K1520007
location: https://synbiohub.org/public/igem/BBa_K1520007/annotation2384924/range2384924/1
component: BBa_R0051

https://synbiohub.org/public/igem/BBa_K1520007/annotation2384924/range2384924/1
type: Range
persistent identity: range2384924
display id: range2384924
version: 1
direction: direction/forward
top level: BBa_K1520007
start: 1
end: 49
orientation: inline

BBa_P0451
type: ComponentDefinition
persistent identity: BBa P0451
display id: BBa_P0451
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PoPS -> cI (lambda) [S0153]
creator: Randy Rettberg
created: 2004-04-26T11:00:00Z
modified: 2015-05-08T01:14:11Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_C0051
component: BBa_B0012
component: BBa_B0010
component: BBa_B0034
sequence annotation: BBa_B0012
sequence annotation: BBa_B0010
sequence annotation: BBa_B0034
sequence annotation: BBa_C0051
sequence: https://synbiohub.org/public/igem/BBa_P0451_sequence/1

BBa_C0051
type: Component
persistent identity: component944967
display id: component944967
version: 1
top level: BBa_P0451
access: public
definition: cI lam

BBa_B0012
type: Component
persistent identity: component944983
display id: component944983
version: 1
top level: BBa_P0451
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0010
type: Component
persistent identity: component944973
display id: component944973
version: 1
top level: BBa_P0451
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0034
type: Component
persistent identity: component944957
display id: component944957
version: 1
top level: BBa_P0451
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation944983
display id: annotation944983
version: 1
top level: BBa_P0451
location: https://synbiohub.org/public/igem/BBa_P0451/annotation944983/range944983/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_P0451/annotation944983/range944983/1
type: Range
persistent identity: range944983
display id: range944983
version: 1
direction: direction/forward
top level: BBa_P0451
start: 890
end: 930
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation944973
display id: annotation944973
version: 1
top level: BBa_P0451
location: https://synbiohub.org/public/igem/BBa_P0451/annotation944973/range944973/1
component: BBa_B0010

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type: Range
persistent identity: range944973
display id: range944973
version: 1
direction: direction/forward
top level: BBa_P0451
start: 802
end: 881
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation944957
display id: annotation944957
version: 1
top level: BBa_P0451
location: https://synbiohub.org/public/igem/BBa_P0451/annotation944957/range944957/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_P0451/annotation944957/range944957/1
type: Range
persistent identity: range944957
display id: range944957
version: 1
direction: direction/forward
top level: BBa_P0451
start: 1
end: 12
orientation: inline

BBa_C0051
type: SequenceAnnotation
persistent identity: annotation944967
display id: annotation944967
version: 1
top level: BBa_P0451
location: https://synbiohub.org/public/igem/BBa_P0451/annotation944967/range944967/1
component: BBa_C0051

https://synbiohub.org/public/igem/BBa_P0451/annotation944967/range944967/1
type: Range
persistent identity: range944967
display id: range944967
version: 1
direction: direction/forward
top level: BBa_P0451
start: 19
end: 768
orientation: inline

BBa_K142076
type: ComponentDefinition
persistent identity: BBa K142076
display id: BBa_K142076
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: lac-controlled RFP generator with constitutive lacI expression cassette
creator: Julius Rabl
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:10:22Z
discontinued: false
dominant: false
group u list: _194_
m user id: 0
owner id: 3254
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: lac-controlled RFP generator with constitutive lacI expression cassette
mutable notes: We put the LacI generator behind the lac-controlled expression cassette to minimze readthrough.
mutable provenance: Subcloned from BioBricks using standard molecular biology techniques.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_J04450
component: BBa_I739002
sequence annotation: BBa_I739002
sequence annotation: BBa_J04450
sequence: https://synbiohub.org/public/igem/BBa_K142076_sequence/1

BBa_J04450
type: Component
persistent identity: component2283059
display id: component2283059
version: 1
top level: BBa_K142076
access: public
definition: https://synbiohub.org/public/igem/BBa_J04450/1

BBa_I739002
type: Component
persistent identity: component2283074
display id: component2283074
version: 1
top level: BBa_K142076
access: public
definition: https://synbiohub.org/public/igem/BBa_I739002/1

BBa_I739002
type: SequenceAnnotation
persistent identity: annotation2283074
display id: annotation2283074
version: 1
top level: BBa_K142076
location: https://synbiohub.org/public/igem/BBa_K142076/annotation2283074/range2283074/1
component: BBa_I739002

https://synbiohub.org/public/igem/BBa_K142076/annotation2283074/range2283074/1
type: Range
persistent identity: range2283074
display id: range2283074
version: 1
direction: direction/reverse
top level: BBa_K142076
start: 1078
end: 2428
orientation: inline

BBa_J04450
type: SequenceAnnotation
persistent identity: annotation2283059
display id: annotation2283059
version: 1
top level: BBa_K142076
location: https://synbiohub.org/public/igem/BBa_K142076/annotation2283059/range2283059/1
component: BBa_J04450

https://synbiohub.org/public/igem/BBa_K142076/annotation2283059/range2283059/1
type: Range
persistent identity: range2283059
display id: range2283059
version: 1
direction: direction/reverse
top level: BBa_K142076
start: 1
end: 1069
orientation: inline

BBa_K404100
type: ComponentDefinition
persistent identity: BBa K404100
display id: BBa_K404100
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: [AAV2]-left-ITR
creator: Freiburg Bioware 2010
created: 2010-08-27T11:00:00Z
modified: 2015-05-08T01:12:23Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 7049
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: misc
sequence: https://synbiohub.org/public/igem/BBa_K404100_sequence/1

misc
type: SequenceAnnotation
persistent identity: annotation2093166
display id: annotation2093166
version: 1
top level: BBa_K404100
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404100/annotation2093166/range2093166/1

https://synbiohub.org/public/igem/BBa_K404100/annotation2093166/range2093166/1
type: Range
persistent identity: range2093166
display id: range2093166
version: 1
direction: direction/forward
top level: BBa_K404100
start: 1
end: 147
orientation: inline

CspA
type: ComponentDefinition
persistent identity: BBa K538003
display id: BBa_K538003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CspA (P. irgensii)
creator: Bas Stringer (Sequence by Paul van Dieken)
created: 2011-09-07T11:00:00Z
modified: 2015-05-08T01:12:36Z
discontinued: false
dominant: false
experience: experience/None
group u list: _704_
m user id: 0
owner id: 9985
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: -
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: RNA binding motif (RPN1)
sequence annotation: Start Codon
sequence annotation: Double Stop Codon
sequence annotation: RNA binding motif (RPN2)
sequence: https://synbiohub.org/public/igem/BBa_K538003_sequence/1

RNA binding motif (RPN1)
type: SequenceAnnotation
persistent identity: annotation2129603
display id: annotation2129603
version: 1
top level: CspA
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K538003/annotation2129603/range2129603/1

https://synbiohub.org/public/igem/BBa_K538003/annotation2129603/range2129603/1
type: Range
persistent identity: range2129603
display id: range2129603
version: 1
direction: direction/forward
top level: CspA
start: 37
end: 54
orientation: inline

Start Codon
type: SequenceAnnotation
persistent identity: annotation2127781
display id: annotation2127781
version: 1
top level: CspA
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K538003/annotation2127781/range2127781/1

https://synbiohub.org/public/igem/BBa_K538003/annotation2127781/range2127781/1
type: Range
persistent identity: range2127781
display id: range2127781
version: 1
direction: direction/forward
top level: CspA
start: 1
end: 3
orientation: inline

Double Stop Codon
type: SequenceAnnotation
persistent identity: annotation2127782
display id: annotation2127782
version: 1
top level: CspA
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K538003/annotation2127782/range2127782/1

https://synbiohub.org/public/igem/BBa_K538003/annotation2127782/range2127782/1
type: Range
persistent identity: range2127782
display id: range2127782
version: 1
direction: direction/forward
top level: CspA
start: 190
end: 195
orientation: inline

RNA binding motif (RPN2)
type: SequenceAnnotation
persistent identity: annotation2129604
display id: annotation2129604
version: 1
top level: CspA
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K538003/annotation2129604/range2129604/1

https://synbiohub.org/public/igem/BBa_K538003/annotation2129604/range2129604/1
type: Range
persistent identity: range2129604
display id: range2129604
version: 1
direction: direction/forward
top level: CspA
start: 79
end: 93
orientation: inline

BBa_K360121
type: ComponentDefinition
persistent identity: BBa K360121
display id: BBa_K360121
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS + LovTAP Photosensor + terminator
creator: Claudia Ivonne Hernandez Armenta
created: 2010-09-08T11:00:00Z
modified: 2015-05-08T01:12:12Z
discontinued: false
dominant: false
experience: experience/Issues
group u list: _485_
m user id: 0
owner id: 6618
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This photoreceptor was assembled by Strickland et al., and consists of a LOV (light-oxygen-voltage) domain of Avena sativa phototropin1 (AsLOV2) that senses blue light, fused to the trpR- DNA binding domain of the transcription factor trp repressor. The resulting protein is named LovTAP: LOV- and tryptophan-activated protein. LOV domains bind a flavin-mononucleotide (FMN) or flavin-adenine-dinucleotide (FAD) cofactor, which are used in a wide variety of metabolic pathways as cofactors in redox reactions and are available in most organisms. The cofactor has a broad absorption spectrum, with a maximum at 450 nm. Besides, the core of the LOV domain is often flanked by amino- or carboxy-terminal helices, termed A???α and Jα, respectively In the LovTAP construction, AsLOV2 domain via its carboxyl-terminal Jα ???helix was ligated to an amino-terminal truncation of TrpR. The resulting protein has a domain-domain overlap with a shared helix. Thus, photoexcitation would change the conformation of the protein, in turn changing the stability of the shared-helix-domain contacts. Under the presence of light, absorption of a photon leads to the formation of a covalent adduct between the flavin mononucleotide (FMN) cofactor and a conserved cysteine residue in the AsLOV2 domain, which results in conformational rearrangements in LovTAP. This change impacts the affinity of the shared helix for the two domains: disrupting the contacts between the shared helix and the LOV domain and enabling the association of the shared helix with the TrpR domain, which establishes DNA-binding affinity and LovTAP can then bind DNA as an homodimer, repressing the transcription of the genes downstream of its binding sites. In the dark, when the shared helix contacts the LOV domain, the TrpR domain's DNA-binding affinity decreases and LovTAP is in an inactive conformation.
mutable notes: We decided to synthesize a new LovTAP part, that in comparison with the Part:BBa_K191003 that is already at the registry, has the following differences: 1. The 2 PstI restriction sites were removed from the coding region of LovTap. 2. We included a punctual mutation to change the ILE427 by a PHE427, as was proposed by the model results of the team iGEM09_EPF-Lausanne. With this mutation LovTAP should react faster and the conformational change should be more stable (the protein stays in the active form for longer, under light induction). 3. The part does not include a promoter. We eliminated the inverting regulator sensitive to LacI and CAP protein(Part:BBa_R0010). According to the report of EPF-Laussane team, the expression levels of LovTAP under the inverting regulator, doesn??t seem to show differences to the induction with IPTG. Thus, we decided to remove it and as we know that the level expression of LovTAP must be low (because at high levels of expresion, there is unspecific regulation under darkness and light states), we plan to test weak promoters to choose the best option and include it in the construction. 4. The RBS was changed from a strong (Part: BBa_B0030) to a medium strength (Part:BBa_B0032), thus expecting lower levels of lovTAP protein. 5. The stop codon tga was changed for two taa.
mutable provenance: Chiameric protein assembled by Strickland et al. Reference: Strickland, D., Moffat, K., & Sosnick, T. (2008). Light-activated DNA binding in a designed allosteric protein. Proceedings of the National Academy of Sciences, 105(31), 10709. National Acad Sciences. Retrieved from http://www.pnas.org/content/105/31/10709.full.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Signalling
role: SO:0000804
sequence annotation: AAA
sequence annotation: stop codon
sequence annotation: RBS B0032
sequence annotation: Terminator B0010
sequence annotation: Terminator B0012
sequence annotation: LovTAP K360022
sequence: https://synbiohub.org/public/igem/BBa_K360121_sequence/1

AAA
type: SequenceAnnotation
persistent identity: annotation2091651
display id: annotation2091651
version: 1
top level: BBa_K360121
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_K360121/annotation2091651/range2091651/1

https://synbiohub.org/public/igem/BBa_K360121/annotation2091651/range2091651/1
type: Range
persistent identity: range2091651
display id: range2091651
version: 1
direction: direction/forward
top level: BBa_K360121
start: 831
end: 844
orientation: inline

stop codon
type: SequenceAnnotation
persistent identity: annotation2091647
display id: annotation2091647
version: 1
top level: BBa_K360121
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K360121/annotation2091647/range2091647/1

https://synbiohub.org/public/igem/BBa_K360121/annotation2091647/range2091647/1
type: Range
persistent identity: range2091647
display id: range2091647
version: 1
direction: direction/forward
top level: BBa_K360121
start: 702
end: 706
orientation: inline

RBS B0032
type: SequenceAnnotation
persistent identity: annotation2091646
display id: annotation2091646
version: 1
top level: BBa_K360121
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K360121/annotation2091646/range2091646/1

https://synbiohub.org/public/igem/BBa_K360121/annotation2091646/range2091646/1
type: Range
persistent identity: range2091646
display id: range2091646
version: 1
direction: direction/forward
top level: BBa_K360121
start: 1
end: 13
orientation: inline

Terminator B0010
type: SequenceAnnotation
persistent identity: annotation2091649
display id: annotation2091649
version: 1
top level: BBa_K360121
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K360121/annotation2091649/range2091649/1

https://synbiohub.org/public/igem/BBa_K360121/annotation2091649/range2091649/1
type: Range
persistent identity: range2091649
display id: range2091649
version: 1
direction: direction/forward
top level: BBa_K360121
start: 725
end: 771
orientation: inline

Terminator B0012
type: SequenceAnnotation
persistent identity: annotation2091650
display id: annotation2091650
version: 1
top level: BBa_K360121
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K360121/annotation2091650/range2091650/1

https://synbiohub.org/public/igem/BBa_K360121/annotation2091650/range2091650/1
type: Range
persistent identity: range2091650
display id: range2091650
version: 1
direction: direction/forward
top level: BBa_K360121
start: 804
end: 830
orientation: inline

LovTAP K360022
type: SequenceAnnotation
persistent identity: annotation2091648
display id: annotation2091648
version: 1
top level: BBa_K360121
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K360121/annotation2091648/range2091648/1

https://synbiohub.org/public/igem/BBa_K360121/annotation2091648/range2091648/1
type: Range
persistent identity: range2091648
display id: range2091648
version: 1
direction: direction/forward
top level: BBa_K360121
start: 18
end: 701
orientation: inline

RBS.TetR
type: ComponentDefinition
persistent identity: BBa I15040
display id: BBa_I15040
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS.TetR from pASKIBA3
creator: Jeff Tabor
created: 2005-06-01T11:00:00Z
modified: 2015-08-31T04:07:39Z
discontinued: false
dominant: false
experience: experience/None
group u list: _5_
m user id: 0
owner id: 88
owning group id: 37
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: The part accepts POPS to produce TetR. This sequence encodes the ribosome binding site and tetracycline repressor protein from the plasmid pASKIBA3.
mutable provenance: pASKIBA3 (IBA biotagnology)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
sequence annotation: paskiba3_rbs
sequence annotation: paskiba3_TetR
sequence: https://synbiohub.org/public/igem/BBa_I15040_sequence/1

paskiba3_rbs
type: SequenceAnnotation
persistent identity: annotation1501145
display id: annotation1501145
version: 1
top level: RBS.TetR
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_I15040/annotation1501145/range1501145/1

https://synbiohub.org/public/igem/BBa_I15040/annotation1501145/range1501145/1
type: Range
persistent identity: range1501145
display id: range1501145
version: 1
direction: direction/forward
top level: RBS.TetR
start: 1
end: 15
orientation: inline

paskiba3_TetR
type: SequenceAnnotation
persistent identity: annotation1501146
display id: annotation1501146
version: 1
top level: RBS.TetR
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_I15040/annotation1501146/range1501146/1

https://synbiohub.org/public/igem/BBa_I15040/annotation1501146/range1501146/1
type: Range
persistent identity: range1501146
display id: range1501146
version: 1
direction: direction/forward
top level: RBS.TetR
start: 16
end: 642
orientation: inline

BBa_K608351
type: ComponentDefinition
persistent identity: BBa K608351
display id: BBa_K608351
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: (correct) temperature sensitive promoter
creator: Theodhor Tiko
created: 2011-09-13T11:00:00Z
modified: 2015-05-08T01:12:52Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _780_
m user id: 0
owner id: 9113
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: correct temperatursesitive promotor
mutable notes: asd
mutable provenance: asd
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: OR1
sequence annotation: BBa_J23114
sequence annotation: BBa_K098997
sequence annotation: BBa_R0051
sequence annotation: BBa_B0011
sequence annotation: stem_loop
sequence annotation: -35
sequence annotation: polya
sequence annotation: cIts
sequence annotation: BBa_B0034
sequence annotation: stop
sequence annotation: conserved
sequence annotation: -10
sequence annotation: BBa_B0012
sequence annotation: OR2
sequence annotation: T7 TE
sequence: https://synbiohub.org/public/igem/BBa_K608351_sequence/1

OR1
type: SequenceAnnotation
persistent identity: annotation2131314
display id: annotation2131314
version: 1
top level: BBa_K608351
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K608351/annotation2131314/range2131314/1

https://synbiohub.org/public/igem/BBa_K608351/annotation2131314/range2131314/1
type: Range
persistent identity: range2131314
display id: range2131314
version: 1
direction: direction/forward
top level: BBa_K608351
start: 924
end: 940
orientation: inline

BBa_J23114
type: SequenceAnnotation
persistent identity: annotation2131300
display id: annotation2131300
version: 1
top level: BBa_K608351
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K608351/annotation2131300/range2131300/1

https://synbiohub.org/public/igem/BBa_K608351/annotation2131300/range2131300/1
type: Range
persistent identity: range2131300
display id: range2131300
version: 1
direction: direction/reverse
top level: BBa_K608351
start: 1
end: 35
orientation: inline

BBa_K098997
type: SequenceAnnotation
persistent identity: annotation2131304
display id: annotation2131304
version: 1
top level: BBa_K608351
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K608351/annotation2131304/range2131304/1

https://synbiohub.org/public/igem/BBa_K608351/annotation2131304/range2131304/1
type: Range
persistent identity: range2131304
display id: range2131304
version: 1
direction: direction/reverse
top level: BBa_K608351
start: 62
end: 775
orientation: inline

BBa_R0051
type: SequenceAnnotation
persistent identity: annotation2131312
display id: annotation2131312
version: 1
top level: BBa_K608351
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K608351/annotation2131312/range2131312/1

https://synbiohub.org/public/igem/BBa_K608351/annotation2131312/range2131312/1
type: Range
persistent identity: range2131312
display id: range2131312
version: 1
direction: direction/forward
top level: BBa_K608351
start: 900
end: 948
orientation: inline

BBa_B0011
type: SequenceAnnotation
persistent identity: annotation2131309
display id: annotation2131309
version: 1
top level: BBa_K608351
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K608351/annotation2131309/range2131309/1

https://synbiohub.org/public/igem/BBa_K608351/annotation2131309/range2131309/1
type: Range
persistent identity: range2131309
display id: range2131309
version: 1
direction: direction/forward
top level: BBa_K608351
start: 846
end: 891
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation2131310
display id: annotation2131310
version: 1
top level: BBa_K608351
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K608351/annotation2131310/range2131310/1

https://synbiohub.org/public/igem/BBa_K608351/annotation2131310/range2131310/1
type: Range
persistent identity: range2131310
display id: range2131310
version: 1
direction: direction/forward
top level: BBa_K608351
start: 858
end: 880
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2131313
display id: annotation2131313
version: 1
top level: BBa_K608351
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K608351/annotation2131313/range2131313/1

https://synbiohub.org/public/igem/BBa_K608351/annotation2131313/range2131313/1
type: Range
persistent identity: range2131313
display id: range2131313
version: 1
direction: direction/forward
top level: BBa_K608351
start: 914
end: 919
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation2131307
display id: annotation2131307
version: 1
top level: BBa_K608351
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_K608351/annotation2131307/range2131307/1

https://synbiohub.org/public/igem/BBa_K608351/annotation2131307/range2131307/1
type: Range
persistent identity: range2131307
display id: range2131307
version: 1
direction: direction/forward
top level: BBa_K608351
start: 824
end: 837
orientation: inline

cIts
type: SequenceAnnotation
persistent identity: annotation2131303
display id: annotation2131303
version: 1
top level: BBa_K608351
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K608351/annotation2131303/range2131303/1

https://synbiohub.org/public/igem/BBa_K608351/annotation2131303/range2131303/1
type: Range
persistent identity: range2131303
display id: range2131303
version: 1
direction: direction/forward
top level: BBa_K608351
start: 62
end: 775
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2131302
display id: annotation2131302
version: 1
top level: BBa_K608351
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K608351/annotation2131302/range2131302/1

https://synbiohub.org/public/igem/BBa_K608351/annotation2131302/range2131302/1
type: Range
persistent identity: range2131302
display id: range2131302
version: 1
direction: direction/reverse
top level: BBa_K608351
start: 44
end: 55
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2131308
display id: annotation2131308
version: 1
top level: BBa_K608351
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K608351/annotation2131308/range2131308/1

https://synbiohub.org/public/igem/BBa_K608351/annotation2131308/range2131308/1
type: Range
persistent identity: range2131308
display id: range2131308
version: 1
direction: direction/forward
top level: BBa_K608351
start: 830
end: 830
orientation: inline

conserved
type: SequenceAnnotation
persistent identity: annotation2131301
display id: annotation2131301
version: 1
top level: BBa_K608351
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K608351/annotation2131301/range2131301/1

https://synbiohub.org/public/igem/BBa_K608351/annotation2131301/range2131301/1
type: Range
persistent identity: range2131301
display id: range2131301
version: 1
direction: direction/forward
top level: BBa_K608351
start: 48
end: 51
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2131315
display id: annotation2131315
version: 1
top level: BBa_K608351
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K608351/annotation2131315/range2131315/1

https://synbiohub.org/public/igem/BBa_K608351/annotation2131315/range2131315/1
type: Range
persistent identity: range2131315
display id: range2131315
version: 1
direction: direction/forward
top level: BBa_K608351
start: 937
end: 942
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2131305
display id: annotation2131305
version: 1
top level: BBa_K608351
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K608351/annotation2131305/range2131305/1

https://synbiohub.org/public/igem/BBa_K608351/annotation2131305/range2131305/1
type: Range
persistent identity: range2131305
display id: range2131305
version: 1
direction: direction/forward
top level: BBa_K608351
start: 797
end: 837
orientation: inline

OR2
type: SequenceAnnotation
persistent identity: annotation2131311
display id: annotation2131311
version: 1
top level: BBa_K608351
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K608351/annotation2131311/range2131311/1

https://synbiohub.org/public/igem/BBa_K608351/annotation2131311/range2131311/1
type: Range
persistent identity: range2131311
display id: range2131311
version: 1
direction: direction/forward
top level: BBa_K608351
start: 900
end: 916
orientation: inline

conserved
type: SequenceAnnotation
persistent identity: annotation2131425
display id: annotation2131425
version: 1
top level: BBa_K608351
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K608351/annotation2131425/range2131425/1

https://synbiohub.org/public/igem/BBa_K608351/annotation2131425/range2131425/1
type: Range
persistent identity: range2131425
display id: range2131425
version: 1
direction: direction/forward
top level: BBa_K608351
start: 776
end: 788
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation2131306
display id: annotation2131306
version: 1
top level: BBa_K608351
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K608351/annotation2131306/range2131306/1

https://synbiohub.org/public/igem/BBa_K608351/annotation2131306/range2131306/1
type: Range
persistent identity: range2131306
display id: range2131306
version: 1
direction: direction/forward
top level: BBa_K608351
start: 804
end: 823
orientation: inline

BBa_K346000
type: ComponentDefinition
persistent identity: BBa K346000
display id: BBa_K346000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS(B0032)+T3 DNA-directed RNA polymerase
creator: Haoqian Zhang and Weiye Wang
created: 2010-09-24T11:00:00Z
modified: 2015-05-08T01:12:08Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _457_
m user id: 0
owner id: 4411
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: This part is the coding region of MerR, which is a mercury responsive regulator from Tn21.
mutable notes: We cloned this part from plasmid NR1, which was a gift from Anne O. Summers.
mutable provenance: Tn21
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
sequence annotation: t3 pol
sequence annotation: b0032
sequence: https://synbiohub.org/public/igem/BBa_K346000_sequence/1

t3 pol
type: SequenceAnnotation
persistent identity: annotation2092859
display id: annotation2092859
version: 1
top level: BBa_K346000
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K346000/annotation2092859/range2092859/1

https://synbiohub.org/public/igem/BBa_K346000/annotation2092859/range2092859/1
type: Range
persistent identity: range2092859
display id: range2092859
version: 1
direction: direction/forward
top level: BBa_K346000
start: 19
end: 2674
orientation: inline

b0032
type: SequenceAnnotation
persistent identity: annotation2092860
display id: annotation2092860
version: 1
top level: BBa_K346000
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K346000/annotation2092860/range2092860/1

https://synbiohub.org/public/igem/BBa_K346000/annotation2092860/range2092860/1
type: Range
persistent identity: range2092860
display id: range2092860
version: 1
direction: direction/forward
top level: BBa_K346000
start: 1
end: 14
orientation: inline

BBa_K1520520
type: ComponentDefinition
persistent identity: BBa K1520520
display id: BBa_K1520520
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PgolTS-golS-PgolB-tetR-Ter-PtetO-rfp-Ter-Plac-tetR-Ter
creator: yue wu
created: 2014-09-25T11:00:00Z
modified: 2015-05-08T01:10:49Z
discontinued: false
dominant: false
group u list: _1902_
m user id: 0
owner id: 21318
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: PgolTS-golS-PgolB-tetR-Ter-PtetO-rfp-Ter-Plac-tetR-Ter It is part of our test of suicide circuit by gold ions.
mutable notes: None
mutable provenance: PgolTS-golS-PgolB, tetR, PtetO, rfp, Plac, Ter
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1520014
component: BBa_K1520518
component: BBa_K1520015
sequence annotation: BBa_K1520014
sequence annotation: BBa_K1520015
sequence annotation: BBa_K1520518
sequence: https://synbiohub.org/public/igem/BBa_K1520520_sequence/1

BBa_K1520014
type: Component
persistent identity: component2386979
display id: component2386979
version: 1
top level: BBa_K1520520
access: public
definition: https://synbiohub.org/public/igem/BBa_K1520014/1

BBa_K1520518
type: Component
persistent identity: component2386971
display id: component2386971
version: 1
top level: BBa_K1520520
access: public
definition: BBa_K1520518

BBa_K1520015
type: Component
persistent identity: component2386991
display id: component2386991
version: 1
top level: BBa_K1520520
access: public
definition: BBa_K1520015

BBa_K1520014
type: SequenceAnnotation
persistent identity: annotation2386979
display id: annotation2386979
version: 1
top level: BBa_K1520520
location: https://synbiohub.org/public/igem/BBa_K1520520/annotation2386979/range2386979/1
component: BBa_K1520014

https://synbiohub.org/public/igem/BBa_K1520520/annotation2386979/range2386979/1
type: Range
persistent identity: range2386979
display id: range2386979
version: 1
direction: direction/reverse
top level: BBa_K1520520
start: 1610
end: 2540
orientation: inline

BBa_K1520015
type: SequenceAnnotation
persistent identity: annotation2386991
display id: annotation2386991
version: 1
top level: BBa_K1520520
location: https://synbiohub.org/public/igem/BBa_K1520520/annotation2386991/range2386991/1
component: BBa_K1520015

https://synbiohub.org/public/igem/BBa_K1520520/annotation2386991/range2386991/1
type: Range
persistent identity: range2386991
display id: range2386991
version: 1
direction: direction/reverse
top level: BBa_K1520520
start: 2549
end: 3596
orientation: inline

BBa_K1520518
type: SequenceAnnotation
persistent identity: annotation2386971
display id: annotation2386971
version: 1
top level: BBa_K1520520
location: https://synbiohub.org/public/igem/BBa_K1520520/annotation2386971/range2386971/1
component: BBa_K1520518

https://synbiohub.org/public/igem/BBa_K1520520/annotation2386971/range2386971/1
type: Range
persistent identity: range2386971
display id: range2386971
version: 1
direction: direction/reverse
top level: BBa_K1520520
start: 1
end: 1601
orientation: inline

BBa_P0340
type: ComponentDefinition
persistent identity: BBa P0340
display id: BBa_P0340
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PoPS -> TetR [S0157]
creator: Randy Rettberg
created: 2004-04-26T11:00:00Z
modified: 2015-05-08T01:14:10Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0012
component: BBa_B0033
component: BBa_B0010
component: BBa_C0040
sequence annotation: BBa_B0033
sequence annotation: BBa_B0012
sequence annotation: BBa_B0010
sequence annotation: BBa_C0040
sequence: https://synbiohub.org/public/igem/BBa_P0340_sequence/1

BBa_B0012
type: Component
persistent identity: component944669
display id: component944669
version: 1
top level: BBa_P0340
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0033
type: Component
persistent identity: component944642
display id: component944642
version: 1
top level: BBa_P0340
access: public
definition: https://synbiohub.org/public/igem/BBa_B0033/1

BBa_B0010
type: Component
persistent identity: component944659
display id: component944659
version: 1
top level: BBa_P0340
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_C0040
type: Component
persistent identity: component944653
display id: component944653
version: 1
top level: BBa_P0340
access: public
definition: tetR

BBa_B0033
type: SequenceAnnotation
persistent identity: annotation944642
display id: annotation944642
version: 1
top level: BBa_P0340
location: https://synbiohub.org/public/igem/BBa_P0340/annotation944642/range944642/1
component: BBa_B0033

https://synbiohub.org/public/igem/BBa_P0340/annotation944642/range944642/1
type: Range
persistent identity: range944642
display id: range944642
version: 1
direction: direction/forward
top level: BBa_P0340
start: 1
end: 11
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation944669
display id: annotation944669
version: 1
top level: BBa_P0340
location: https://synbiohub.org/public/igem/BBa_P0340/annotation944669/range944669/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_P0340/annotation944669/range944669/1
type: Range
persistent identity: range944669
display id: range944669
version: 1
direction: direction/forward
top level: BBa_P0340
start: 799
end: 839
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation944659
display id: annotation944659
version: 1
top level: BBa_P0340
location: https://synbiohub.org/public/igem/BBa_P0340/annotation944659/range944659/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_P0340/annotation944659/range944659/1
type: Range
persistent identity: range944659
display id: range944659
version: 1
direction: direction/forward
top level: BBa_P0340
start: 711
end: 790
orientation: inline

BBa_C0040
type: SequenceAnnotation
persistent identity: annotation944653
display id: annotation944653
version: 1
top level: BBa_P0340
location: https://synbiohub.org/public/igem/BBa_P0340/annotation944653/range944653/1
component: BBa_C0040

https://synbiohub.org/public/igem/BBa_P0340/annotation944653/range944653/1
type: Range
persistent identity: range944653
display id: range944653
version: 1
direction: direction/forward
top level: BBa_P0340
start: 18
end: 677
orientation: inline

BBa_K165076
type: ComponentDefinition
persistent identity: BBa K165076
display id: BBa_K165076
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pMET25 + YY1 repressor on pRS304*
creator: John Szymanski
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:10:57Z
discontinued: false
dominant: false
group u list: _267_
m user id: 0
owner id: 2512
owning group id: 58
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: -
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
sequence: https://synbiohub.org/public/igem/BBa_K165076_sequence/1

BBa_I726031
type: ComponentDefinition
persistent identity: BBa I726031
display id: BBa_I726031
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Trc-LIC
creator: Bangalore iGEM
created: 2007-07-03T11:00:00Z
modified: 2015-08-31T04:07:55Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _107_
m user id: 0
owner id: 2077
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Abcdefghijkl..
mutable notes: none
mutable provenance: Registry
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_R0011
component: BBa_B0034
component: BBa_B0010
component: BBa_C0161
component: BBa_B0012
component: BBa_E0020
sequence annotation: BBa_B0010
sequence annotation: BBa_C0161
sequence annotation: BBa_B0034
sequence annotation: BBa_B0012
sequence annotation: BBa_E0020
sequence annotation: BBa_R0011
sequence: https://synbiohub.org/public/igem/BBa_I726031_sequence/1

BBa_R0011
type: Component
persistent identity: component1951356
display id: component1951356
version: 1
top level: BBa_I726031
access: public
definition: https://synbiohub.org/public/igem/BBa_R0011/1

BBa_B0034
type: Component
persistent identity: component1951362
display id: component1951362
version: 1
top level: BBa_I726031
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0034
type: Component
persistent identity: component1951366
display id: component1951366
version: 1
top level: BBa_I726031
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0010
type: Component
persistent identity: component1951368
display id: component1951368
version: 1
top level: BBa_I726031
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_C0161
type: Component
persistent identity: component1951364
display id: component1951364
version: 1
top level: BBa_I726031
access: public
definition: https://synbiohub.org/public/igem/BBa_C0161/1

BBa_B0012
type: Component
persistent identity: component1951370
display id: component1951370
version: 1
top level: BBa_I726031
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_E0020
type: Component
persistent identity: component1951367
display id: component1951367
version: 1
top level: BBa_I726031
access: public
definition: https://synbiohub.org/public/igem/BBa_E0020/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1951368
display id: annotation1951368
version: 1
top level: BBa_I726031
location: https://synbiohub.org/public/igem/BBa_I726031/annotation1951368/range1951368/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I726031/annotation1951368/range1951368/1
type: Range
persistent identity: range1951368
display id: range1951368
version: 1
direction: direction/forward
top level: BBa_I726031
start: 1424
end: 1503
orientation: inline

BBa_C0161
type: SequenceAnnotation
persistent identity: annotation1951364
display id: annotation1951364
version: 1
top level: BBa_I726031
location: https://synbiohub.org/public/igem/BBa_I726031/annotation1951364/range1951364/1
component: BBa_C0161

https://synbiohub.org/public/igem/BBa_I726031/annotation1951364/range1951364/1
type: Range
persistent identity: range1951364
display id: range1951364
version: 1
direction: direction/reverse
top level: BBa_I726031
start: 82
end: 666
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1951366
display id: annotation1951366
version: 1
top level: BBa_I726031
location: https://synbiohub.org/public/igem/BBa_I726031/annotation1951366/range1951366/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I726031/annotation1951366/range1951366/1
type: Range
persistent identity: range1951366
display id: range1951366
version: 1
direction: direction/reverse
top level: BBa_I726031
start: 675
end: 686
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1951362
display id: annotation1951362
version: 1
top level: BBa_I726031
location: https://synbiohub.org/public/igem/BBa_I726031/annotation1951362/range1951362/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I726031/annotation1951362/range1951362/1
type: Range
persistent identity: range1951362
display id: range1951362
version: 1
direction: direction/reverse
top level: BBa_I726031
start: 64
end: 75
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1951370
display id: annotation1951370
version: 1
top level: BBa_I726031
location: https://synbiohub.org/public/igem/BBa_I726031/annotation1951370/range1951370/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I726031/annotation1951370/range1951370/1
type: Range
persistent identity: range1951370
display id: range1951370
version: 1
direction: direction/forward
top level: BBa_I726031
start: 1512
end: 1552
orientation: inline

BBa_E0020
type: SequenceAnnotation
persistent identity: annotation1951367
display id: annotation1951367
version: 1
top level: BBa_I726031
location: https://synbiohub.org/public/igem/BBa_I726031/annotation1951367/range1951367/1
component: BBa_E0020

https://synbiohub.org/public/igem/BBa_I726031/annotation1951367/range1951367/1
type: Range
persistent identity: range1951367
display id: range1951367
version: 1
direction: direction/reverse
top level: BBa_I726031
start: 693
end: 1415
orientation: inline

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation1951356
display id: annotation1951356
version: 1
top level: BBa_I726031
location: https://synbiohub.org/public/igem/BBa_I726031/annotation1951356/range1951356/1
component: BBa_R0011

https://synbiohub.org/public/igem/BBa_I726031/annotation1951356/range1951356/1
type: Range
persistent identity: range1951356
display id: range1951356
version: 1
direction: direction/forward
top level: BBa_I726031
start: 1
end: 54
orientation: inline

BBa_K165079
type: ComponentDefinition
persistent identity: BBa K165079
display id: BBa_K165079
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pGAL1 + Null synthetic activator on pRS305
creator: Aaron Glieberman
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:10:57Z
discontinued: false
dominant: false
group u list: _267_
m user id: 0
owner id: 2510
owning group id: 58
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Note that this is a ligation that we made onto a pRS305 vector, which does not conform to Biobrick standard. As such, there is some remnant of the Biobrick ends but
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_K165029
component: BBa_K165046
sequence annotation: BBa_K165046
sequence annotation: BBa_K165029
sequence: https://synbiohub.org/public/igem/BBa_K165079_sequence/1

BBa_K165029
type: Component
persistent identity: component1997120
display id: component1997120
version: 1
top level: BBa_K165079
access: public
definition: https://synbiohub.org/public/igem/BBa_K165029/1

BBa_K165046
type: Component
persistent identity: component1997119
display id: component1997119
version: 1
top level: BBa_K165079
access: public
definition: https://synbiohub.org/public/igem/BBa_K165046/1

BBa_K165046
type: SequenceAnnotation
persistent identity: annotation1997119
display id: annotation1997119
version: 1
top level: BBa_K165079
location: https://synbiohub.org/public/igem/BBa_K165079/annotation1997119/range1997119/1
component: BBa_K165046

https://synbiohub.org/public/igem/BBa_K165079/annotation1997119/range1997119/1
type: Range
persistent identity: range1997119
display id: range1997119
version: 1
direction: direction/reverse
top level: BBa_K165079
start: 1
end: 549
orientation: inline

BBa_K165029
type: SequenceAnnotation
persistent identity: annotation1997120
display id: annotation1997120
version: 1
top level: BBa_K165079
location: https://synbiohub.org/public/igem/BBa_K165079/annotation1997120/range1997120/1
component: BBa_K165029

https://synbiohub.org/public/igem/BBa_K165079/annotation1997120/range1997120/1
type: Range
persistent identity: range1997120
display id: range1997120
version: 1
direction: direction/reverse
top level: BBa_K165079
start: 558
end: 2447
orientation: inline

BBa_K137132
type: ComponentDefinition
persistent identity: BBa K137132
display id: BBa_K137132
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Lactose inducible GFP expression
creator: Josh Michener
created: 2008-10-22T11:00:00Z
modified: 2015-05-08T01:10:11Z
discontinued: false
dominant: false
experience: experience/None
group u list: _187_
m user id: 0
owner id: 253
owning group id: 6
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Temp
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_I13504
component: BBa_S04106
sequence annotation: BBa_I13504
sequence annotation: BBa_S04106
sequence: https://synbiohub.org/public/igem/BBa_K137132_sequence/1

BBa_I13504
type: Component
persistent identity: component2282459
display id: component2282459
version: 1
top level: BBa_K137132
access: public
definition: https://synbiohub.org/public/igem/BBa_I13504/1

BBa_S04106
type: Component
persistent identity: component2282449
display id: component2282449
version: 1
top level: BBa_K137132
access: public
definition: https://synbiohub.org/public/igem/BBa_S04106/1

BBa_I13504
type: SequenceAnnotation
persistent identity: annotation2282459
display id: annotation2282459
version: 1
top level: BBa_K137132
location: https://synbiohub.org/public/igem/BBa_K137132/annotation2282459/range2282459/1
component: BBa_I13504

https://synbiohub.org/public/igem/BBa_K137132/annotation2282459/range2282459/1
type: Range
persistent identity: range2282459
display id: range2282459
version: 1
direction: direction/reverse
top level: BBa_K137132
start: 1471
end: 2345
orientation: inline

BBa_S04106
type: SequenceAnnotation
persistent identity: annotation2282449
display id: annotation2282449
version: 1
top level: BBa_K137132
location: https://synbiohub.org/public/igem/BBa_K137132/annotation2282449/range2282449/1
component: BBa_S04106

https://synbiohub.org/public/igem/BBa_K137132/annotation2282449/range2282449/1
type: Range
persistent identity: range2282449
display id: range2282449
version: 1
direction: direction/reverse
top level: BBa_K137132
start: 1
end: 1462
orientation: inline

virG
type: ComponentDefinition
persistent identity: BBa K389002
display id: BBa_K389002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Mutated virG
creator: Jonas Aretz
created: 2010-08-09T11:00:00Z
modified: 2015-05-08T01:12:18Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _517_
m user id: 0
owner id: 6670
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This is the ''virG'' response regulator from the virA/G receptor system. The virA/G receptor system is used by ''Agrobacterium tumefaciens'' to detect phenolic substances secreted by plants. In presence of these substances, ''virG'' is activated by the ''virA'' receptor and induces the transcription of genes under the control of a ''vir'' promotor. Normally, ''virG'' needs the ''rpoA'' RNA polymerase subunit from ''A. tumefaciens'' to work in ''Escherichia coli'' but this ''virG'' BioBrick is mutated, so works with the ''rpoA'' subunit from ''E. coli''.
mutable notes: synthesized by Mr. Gene, so optimated codon usage, removal of illegal restriction sites
mutable provenance: synthetic
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: virG
sequence annotation: RBS
sequence annotation: Stop codon
sequence: https://synbiohub.org/public/igem/BBa_K389002_sequence/1

virG
type: SequenceAnnotation
persistent identity: annotation2078361
display id: annotation2078361
version: 1
top level: virG
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K389002/annotation2078361/range2078361/1

https://synbiohub.org/public/igem/BBa_K389002/annotation2078361/range2078361/1
type: Range
persistent identity: range2078361
display id: range2078361
version: 1
direction: direction/forward
top level: virG
start: 12
end: 737
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2078360
display id: annotation2078360
version: 1
top level: virG
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K389002/annotation2078360/range2078360/1

https://synbiohub.org/public/igem/BBa_K389002/annotation2078360/range2078360/1
type: Range
persistent identity: range2078360
display id: range2078360
version: 1
direction: direction/forward
top level: virG
start: 4
end: 8
orientation: inline

Stop codon
type: SequenceAnnotation
persistent identity: annotation2078362
display id: annotation2078362
version: 1
top level: virG
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K389002/annotation2078362/range2078362/1

https://synbiohub.org/public/igem/BBa_K389002/annotation2078362/range2078362/1
type: Range
persistent identity: range2078362
display id: range2078362
version: 1
direction: direction/forward
top level: virG
start: 738
end: 740
orientation: inline

cI TP901
type: ComponentDefinition
persistent identity: BBa C0075
display id: BBa_C0075
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TP901 cI repressor from phage TP901-1 (+LVA)
creator: crackdots
created: 2004-01-27T12:00:00Z
modified: 2015-08-31T04:07:23Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: -- No description --
mutable notes: Dimeric repressor from bacteriophage TP901-1 with dissociation constant of 2*10^-9. Sequence was translated with the E.Coli K12 codon usage table, and nucleotides 84-86 was changed from TTC to TTT to avoid the cut site GAATTC (81-86). Bronsted, L., Hammer, K., Johansen, A.H., 2003. Identification of operator sites of the CI repressor of the phage TP901-1: evolutionary link to the other phages. Virology 311, 144-156. cI sequence slightly different from part C0051. NCBI locus: NC_001416
mutable provenance: TP901-1
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: LVA
sequence annotation: Help:Barcodes
sequence annotation: cI tp901
sequence: https://synbiohub.org/public/igem/BBa_C0075_sequence/1

LVA
type: SequenceAnnotation
persistent identity: annotation306574
display id: annotation306574
version: 1
top level: cI TP901
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_C0075/annotation306574/range306574/1

https://synbiohub.org/public/igem/BBa_C0075/annotation306574/range306574/1
type: Range
persistent identity: range306574
display id: range306574
version: 1
direction: direction/forward
top level: cI TP901
start: 541
end: 573
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2214007
display id: annotation2214007
version: 1
top level: cI TP901
role: SO:0000807
role: feature/barcode
location: https://synbiohub.org/public/igem/BBa_C0075/annotation2214007/range2214007/1

https://synbiohub.org/public/igem/BBa_C0075/annotation2214007/range2214007/1
type: Range
persistent identity: range2214007
display id: range2214007
version: 1
direction: direction/forward
top level: cI TP901
start: 580
end: 604
orientation: inline

cI tp901
type: SequenceAnnotation
persistent identity: annotation302645
display id: annotation302645
version: 1
top level: cI TP901
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_C0075/annotation302645/range302645/1

https://synbiohub.org/public/igem/BBa_C0075/annotation302645/range302645/1
type: Range
persistent identity: range302645
display id: range302645
version: 1
direction: direction/forward
top level: cI TP901
start: 1
end: 540
orientation: inline

BBa_C0056
type: ComponentDefinition
persistent identity: BBa C0056
display id: BBa_C0056
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: cI repressor from phage 434 (no LVA)
creator: Reshma Shetty
created: 2004-09-01T11:00:00Z
modified: 2015-08-31T04:07:23Z
discontinued: false
dominant: false
experience: experience/None
group u list: _41_6_
m user id: 0
owner id: 126
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: -- No description --
mutable notes: Removed LVA tag from BBa_C0052 via PCR. In pSB1AK3 currently.
mutable provenance: Removed LVA tag from BBa_C0052
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: 2
sequence annotation: cI 434
sequence: https://synbiohub.org/public/igem/BBa_C0056_sequence/1

2
type: SequenceAnnotation
persistent identity: annotation1063930
display id: annotation1063930
version: 1
top level: BBa_C0056
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_C0056/annotation1063930/range1063930/1

https://synbiohub.org/public/igem/BBa_C0056/annotation1063930/range1063930/1
type: Range
persistent identity: range1063930
display id: range1063930
version: 1
direction: direction/forward
top level: BBa_C0056
start: 631
end: 636
orientation: inline

cI 434
type: SequenceAnnotation
persistent identity: annotation1063931
display id: annotation1063931
version: 1
top level: BBa_C0056
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_C0056/annotation1063931/range1063931/1

https://synbiohub.org/public/igem/BBa_C0056/annotation1063931/range1063931/1
type: Range
persistent identity: range1063931
display id: range1063931
version: 1
direction: direction/forward
top level: BBa_C0056
start: 1
end: 636
orientation: inline

TFBS
type: ComponentDefinition
persistent identity: BBa K2127006
display id: BBa_K2127006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Transcription Factor Binding Site from Cyanobacterial Pcpc560
creator: Mirat Sojitra
created: 2016-10-13T11:00:00Z
modified: 2016-10-25T10:25:34Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2596_
m user id: 32415
owner id: 32736
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Pcpc560-TFBS
mutable notes: Pcpc560-TFBS
mutable provenance: Pcpc560-TFBS
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: Transcription Factor Binding Sites
sequence: https://synbiohub.org/public/igem/BBa_K2127006_sequence/1

Transcription Factor Binding Sites
type: SequenceAnnotation
persistent identity: annotation2530242
display id: annotation2530242
version: 1
top level: TFBS
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K2127006/annotation2530242/range2530242/1

https://synbiohub.org/public/igem/BBa_K2127006/annotation2530242/range2530242/1
type: Range
persistent identity: range2530242
display id: range2530242
version: 1
direction: direction/forward
top level: TFBS
start: 1
end: 185
orientation: inline

BBa_P0450
type: ComponentDefinition
persistent identity: BBa P0450
display id: BBa_P0450
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PoPS -> cI (HK022) [S01152]
creator: Randy Rettberg
created: 2004-04-26T11:00:00Z
modified: 2015-05-08T01:14:10Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_C0050
component: BBa_B0012
component: BBa_B0010
component: BBa_B0034
sequence annotation: BBa_B0010
sequence annotation: BBa_C0050
sequence annotation: BBa_B0034
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_P0450_sequence/1

BBa_C0050
type: Component
persistent identity: component944933
display id: component944933
version: 1
top level: BBa_P0450
access: public
definition: cI HK022

BBa_B0012
type: Component
persistent identity: component944951
display id: component944951
version: 1
top level: BBa_P0450
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0010
type: Component
persistent identity: component944941
display id: component944941
version: 1
top level: BBa_P0450
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0034
type: Component
persistent identity: component944918
display id: component944918
version: 1
top level: BBa_P0450
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation944941
display id: annotation944941
version: 1
top level: BBa_P0450
location: https://synbiohub.org/public/igem/BBa_P0450/annotation944941/range944941/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_P0450/annotation944941/range944941/1
type: Range
persistent identity: range944941
display id: range944941
version: 1
direction: direction/forward
top level: BBa_P0450
start: 796
end: 875
orientation: inline

BBa_C0050
type: SequenceAnnotation
persistent identity: annotation944933
display id: annotation944933
version: 1
top level: BBa_P0450
location: https://synbiohub.org/public/igem/BBa_P0450/annotation944933/range944933/1
component: BBa_C0050

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type: Range
persistent identity: range944933
display id: range944933
version: 1
direction: direction/forward
top level: BBa_P0450
start: 19
end: 762
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation944918
display id: annotation944918
version: 1
top level: BBa_P0450
location: https://synbiohub.org/public/igem/BBa_P0450/annotation944918/range944918/1
component: BBa_B0034

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type: Range
persistent identity: range944918
display id: range944918
version: 1
direction: direction/forward
top level: BBa_P0450
start: 1
end: 12
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation944951
display id: annotation944951
version: 1
top level: BBa_P0450
location: https://synbiohub.org/public/igem/BBa_P0450/annotation944951/range944951/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_P0450/annotation944951/range944951/1
type: Range
persistent identity: range944951
display id: range944951
version: 1
direction: direction/forward
top level: BBa_P0450
start: 884
end: 924
orientation: inline

BBa_K1072002
type: ComponentDefinition
persistent identity: BBa K1072002
display id: BBa_K1072002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: NADH dehydrogenase
creator: Yichong wang, Ruichen, Xihao liao and Junjie zhang
created: 2013-08-31T11:00:00Z
modified: 2015-05-08T01:09:01Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1381_
m user id: 0
owner id: 16002
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: BBa_K1072002 is the generator fo NADH dehydrogenase which is an enzyme following chemical reaction: NADH + H+ acceptor \rightleftharpoons NAD+ + reduced acceptor
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0010
component: BBa_K1072001
component: BBa_R0062
component: BBa_B0034
component: BBa_B0012
sequence annotation: BBa_K1072001
sequence annotation: BBa_B0012
sequence annotation: BBa_B0010
sequence annotation: BBa_R0062
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K1072002_sequence/1

BBa_B0010
type: Component
persistent identity: component2334353
display id: component2334353
version: 1
top level: BBa_K1072002
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_K1072001
type: Component
persistent identity: component2334352
display id: component2334352
version: 1
top level: BBa_K1072002
access: public
definition: https://synbiohub.org/public/igem/BBa_K1072001/1

BBa_R0062
type: Component
persistent identity: component2334344
display id: component2334344
version: 1
top level: BBa_K1072002
access: public
definition: https://synbiohub.org/public/igem/BBa_R0062/1

BBa_B0034
type: Component
persistent identity: component2334350
display id: component2334350
version: 1
top level: BBa_K1072002
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0012
type: Component
persistent identity: component2334355
display id: component2334355
version: 1
top level: BBa_K1072002
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_K1072001
type: SequenceAnnotation
persistent identity: annotation2334352
display id: annotation2334352
version: 1
top level: BBa_K1072002
location: https://synbiohub.org/public/igem/BBa_K1072002/annotation2334352/range2334352/1
component: BBa_K1072001

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type: Range
persistent identity: range2334352
display id: range2334352
version: 1
direction: direction/reverse
top level: BBa_K1072002
start: 84
end: 1388
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2334355
display id: annotation2334355
version: 1
top level: BBa_K1072002
location: https://synbiohub.org/public/igem/BBa_K1072002/annotation2334355/range2334355/1
component: BBa_B0012

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type: Range
persistent identity: range2334355
display id: range2334355
version: 1
direction: direction/forward
top level: BBa_K1072002
start: 1485
end: 1525
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2334353
display id: annotation2334353
version: 1
top level: BBa_K1072002
location: https://synbiohub.org/public/igem/BBa_K1072002/annotation2334353/range2334353/1
component: BBa_B0010

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type: Range
persistent identity: range2334353
display id: range2334353
version: 1
direction: direction/forward
top level: BBa_K1072002
start: 1397
end: 1476
orientation: inline

BBa_R0062
type: SequenceAnnotation
persistent identity: annotation2334344
display id: annotation2334344
version: 1
top level: BBa_K1072002
location: https://synbiohub.org/public/igem/BBa_K1072002/annotation2334344/range2334344/1
component: BBa_R0062

https://synbiohub.org/public/igem/BBa_K1072002/annotation2334344/range2334344/1
type: Range
persistent identity: range2334344
display id: range2334344
version: 1
direction: direction/forward
top level: BBa_K1072002
start: 1
end: 55
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2334350
display id: annotation2334350
version: 1
top level: BBa_K1072002
location: https://synbiohub.org/public/igem/BBa_K1072002/annotation2334350/range2334350/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1072002/annotation2334350/range2334350/1
type: Range
persistent identity: range2334350
display id: range2334350
version: 1
direction: direction/reverse
top level: BBa_K1072002
start: 64
end: 75
orientation: inline

BBa_K1520015
type: ComponentDefinition
persistent identity: BBa K1520015
display id: BBa_K1520015
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Plac-rbs-tetR-Ter
creator: yue wu
created: 2014-09-21T11:00:00Z
modified: 2015-05-08T01:10:49Z
discontinued: false
dominant: false
group u list: _1902_
m user id: 0
owner id: 21318
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Plac-rbs-tetR-Ter. It produces tetR when there is enough IPTG or less lacI.
mutable notes: None
mutable provenance: Plac, tetR, Ter
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1520029
component: BBa_K1520505
component: BBa_R0010
sequence annotation: BBa_K1520505
sequence annotation: BBa_R0010
sequence annotation: BBa_K1520029
sequence: https://synbiohub.org/public/igem/BBa_K1520015_sequence/1

BBa_K1520029
type: Component
persistent identity: component2385009
display id: component2385009
version: 1
top level: BBa_K1520015
access: public
definition: https://synbiohub.org/public/igem/BBa_K1520029/1

BBa_K1520505
type: Component
persistent identity: component2385008
display id: component2385008
version: 1
top level: BBa_K1520015
access: public
definition: BBa_K1520505

BBa_R0010
type: Component
persistent identity: component2384999
display id: component2384999
version: 1
top level: BBa_K1520015
access: public
definition: https://synbiohub.org/public/igem/BBa_R0010/1

BBa_K1520505
type: SequenceAnnotation
persistent identity: annotation2385008
display id: annotation2385008
version: 1
top level: BBa_K1520015
location: https://synbiohub.org/public/igem/BBa_K1520015/annotation2385008/range2385008/1
component: BBa_K1520505

https://synbiohub.org/public/igem/BBa_K1520015/annotation2385008/range2385008/1
type: Range
persistent identity: range2385008
display id: range2385008
version: 1
direction: direction/reverse
top level: BBa_K1520015
start: 209
end: 911
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation2384999
display id: annotation2384999
version: 1
top level: BBa_K1520015
location: https://synbiohub.org/public/igem/BBa_K1520015/annotation2384999/range2384999/1
component: BBa_R0010

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type: Range
persistent identity: range2384999
display id: range2384999
version: 1
direction: direction/forward
top level: BBa_K1520015
start: 1
end: 200
orientation: inline

BBa_K1520029
type: SequenceAnnotation
persistent identity: annotation2385009
display id: annotation2385009
version: 1
top level: BBa_K1520015
location: https://synbiohub.org/public/igem/BBa_K1520015/annotation2385009/range2385009/1
component: BBa_K1520029

https://synbiohub.org/public/igem/BBa_K1520015/annotation2385009/range2385009/1
type: Range
persistent identity: range2385009
display id: range2385009
version: 1
direction: direction/reverse
top level: BBa_K1520015
start: 920
end: 1048
orientation: inline

BBa_P0152
type: ComponentDefinition
persistent identity: BBa P0152
display id: BBa_P0152
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PoPS -> cI (434) [S0166]
creator: Randy Rettberg
created: 2004-04-24T11:00:00Z
modified: 2015-05-08T01:14:10Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Protein generator converting TIPS to the protein cI from 434. Used as the input section for Quad Part Inverter Q01520.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0031
component: BBa_C0052
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_C0052
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_B0031
sequence: https://synbiohub.org/public/igem/BBa_P0152_sequence/1

BBa_B0031
type: Component
persistent identity: component944535
display id: component944535
version: 1
top level: BBa_P0152
access: public
definition: https://synbiohub.org/public/igem/BBa_B0031/1

BBa_C0052
type: Component
persistent identity: component944545
display id: component944545
version: 1
top level: BBa_P0152
access: public
definition: cI 434

BBa_B0012
type: Component
persistent identity: component944562
display id: component944562
version: 1
top level: BBa_P0152
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0010
type: Component
persistent identity: component944552
display id: component944552
version: 1
top level: BBa_P0152
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_C0052
type: SequenceAnnotation
persistent identity: annotation944545
display id: annotation944545
version: 1
top level: BBa_P0152
location: https://synbiohub.org/public/igem/BBa_P0152/annotation944545/range944545/1
component: BBa_C0052

https://synbiohub.org/public/igem/BBa_P0152/annotation944545/range944545/1
type: Range
persistent identity: range944545
display id: range944545
version: 1
direction: direction/forward
top level: BBa_P0152
start: 21
end: 689
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation944552
display id: annotation944552
version: 1
top level: BBa_P0152
location: https://synbiohub.org/public/igem/BBa_P0152/annotation944552/range944552/1
component: BBa_B0010

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type: Range
persistent identity: range944552
display id: range944552
version: 1
direction: direction/forward
top level: BBa_P0152
start: 723
end: 802
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation944562
display id: annotation944562
version: 1
top level: BBa_P0152
location: https://synbiohub.org/public/igem/BBa_P0152/annotation944562/range944562/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_P0152/annotation944562/range944562/1
type: Range
persistent identity: range944562
display id: range944562
version: 1
direction: direction/forward
top level: BBa_P0152
start: 811
end: 851
orientation: inline

BBa_B0031
type: SequenceAnnotation
persistent identity: annotation944535
display id: annotation944535
version: 1
top level: BBa_P0152
location: https://synbiohub.org/public/igem/BBa_P0152/annotation944535/range944535/1
component: BBa_B0031

https://synbiohub.org/public/igem/BBa_P0152/annotation944535/range944535/1
type: Range
persistent identity: range944535
display id: range944535
version: 1
direction: direction/forward
top level: BBa_P0152
start: 1
end: 14
orientation: inline

BBa_K1031916
type: ComponentDefinition
persistent identity: BBa K1031916
display id: BBa_K1031916
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: J23114-XylS-Terminator
creator: He Shuaixin
created: 2013-09-08T11:00:00Z
modified: 2015-05-08T01:08:48Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1338_
m user id: 0
owner id: 15843
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: 114-XylS-TT
mutable notes: molecular cloning
mutable provenance: synthesis PSEUDOMONAS
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: J23114
sequence annotation: XylS
sequence annotation: B0015
sequence: https://synbiohub.org/public/igem/BBa_K1031916_sequence/1

J23114
type: SequenceAnnotation
persistent identity: annotation2360184
display id: annotation2360184
version: 1
top level: BBa_K1031916
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1031916/annotation2360184/range2360184/1

https://synbiohub.org/public/igem/BBa_K1031916/annotation2360184/range2360184/1
type: Range
persistent identity: range2360184
display id: range2360184
version: 1
direction: direction/forward
top level: BBa_K1031916
start: 1
end: 35
orientation: inline

XylS
type: SequenceAnnotation
persistent identity: annotation2360185
display id: annotation2360185
version: 1
top level: BBa_K1031916
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1031916/annotation2360185/range2360185/1

https://synbiohub.org/public/igem/BBa_K1031916/annotation2360185/range2360185/1
type: Range
persistent identity: range2360185
display id: range2360185
version: 1
direction: direction/forward
top level: BBa_K1031916
start: 62
end: 1027
orientation: inline

B0015
type: SequenceAnnotation
persistent identity: annotation2360187
display id: annotation2360187
version: 1
top level: BBa_K1031916
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1031916/annotation2360187/range2360187/1

https://synbiohub.org/public/igem/BBa_K1031916/annotation2360187/range2360187/1
type: Range
persistent identity: range2360187
display id: range2360187
version: 1
direction: direction/forward
top level: BBa_K1031916
start: 1034
end: 1162
orientation: inline

BBa_K190023
type: ComponentDefinition
persistent identity: BBa K190023
display id: BBa_K190023
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Arsenic Promoter (ArsR regulated) with own RBS
creator: Michael Verhoeven
created: 2009-08-24T11:00:00Z
modified: 2015-05-08T01:11:15Z
discontinued: false
dominant: false
experience: experience/None
group u list: _306_
m user id: 0
owner id: 4144
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: gxsga
mutable notes: xshsah
mutable provenance: xhashjxsxhas
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -35 and -10 region
sequence annotation: RBS site
sequence: https://synbiohub.org/public/igem/BBa_K190023_sequence/1

-35 and -10 region
type: SequenceAnnotation
persistent identity: annotation2017705
display id: annotation2017705
version: 1
top level: BBa_K190023
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K190023/annotation2017705/range2017705/1

https://synbiohub.org/public/igem/BBa_K190023/annotation2017705/range2017705/1
type: Range
persistent identity: range2017705
display id: range2017705
version: 1
direction: direction/forward
top level: BBa_K190023
start: 38
end: 72
orientation: inline

RBS site
type: SequenceAnnotation
persistent identity: annotation2017706
display id: annotation2017706
version: 1
top level: BBa_K190023
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K190023/annotation2017706/range2017706/1

https://synbiohub.org/public/igem/BBa_K190023/annotation2017706/range2017706/1
type: Range
persistent identity: range2017706
display id: range2017706
version: 1
direction: direction/forward
top level: BBa_K190023
start: 73
end: 81
orientation: inline

BBa_K142027
type: ComponentDefinition
persistent identity: BBa K142027
display id: BBa_K142027
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: IPTG-on tetracycline-off pulse generator with LacI mutant (T276F) and TetR expression cassette
creator: Julius Rabl
created: 2008-10-28T12:00:00Z
modified: 2015-05-08T01:10:22Z
discontinued: false
dominant: false
group u list: _194_
m user id: 0
owner id: 3254
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: ==IPTG-on/tetracycline-off switch circuit== ===Short description:=== This part contains a tetracycline-controlled LacI IS expression cassette followed by a constitutive TetR expression cassette. The LacI IS mutant is almost identical to the LacI transcriptional regulator except for the difference that it is not able to bind IPTG or allolactose due to a mutation; it therefore can not be activated by induction with these substances. Since it recognizes the same motif in the lac promotor region, it strongly represses transcription of all genes regulated by promotors with lacI binding site even if IPTG or allolactose are present. It is used to terminate the expression of proteins under lac control if IPTG can not be removed from the cell rapidly. ===Detailed description:=== ====LacI IS mutants are uninducible repressors of lac-controlled promotors==== Expression of the lac operon in E. coli is tightly controlled by LacI, a protein, which binds to a repressor binding site within the promotor and disables transcription by obscuring the promotor region. When bound to DNA, LacI is in the tetrameric form, which consists of two dimers interacting at the end distal from the DNA binding site. Upon binding of allolactose or IPTG, the tetramer breaks down into two dimers and the affinity for the repressor binding site is greatly reduced; the lacI IPTG complex will diffuse away from the repressor binding site, leaving the promotor accessible. As a result of decades of genetic and structural studies, the function of lacI is now understood on the molecular level (1, 2). Mutational experiments have identified residues, which abolish IPTG response upon mutation (3). Furthermore, the x-ray crystal structure of LacI with bound IPTG has allowed the identification of residues that interact with IPTG and which are promising targets for mutagenesis (1). [[image:jr_pulsegen_1.jpg|frame|none| A LacI repressor tetramer, residues R197 and T276 are shown in red. B IPTG bound to the inducer bindi…
mutable notes: During site-directed mutagenesis, the codons to be mutated were replaced with the most highly utilized codons in E. coli to prevent complications from the use of rare codons. The lacI IS sequences were analyzed for BioBrick restriction sites within the coding sequence to ensure their compatibility.
mutable provenance: The pulse generator precursor has been assembled from tetracycline-controlled lacI generator [http://partsregistry.org/wiki/index.php?title=Part:BBa_K142046 K142046] and the constitutive TetR expression cassette [http://partsregistry.org/Part:BBa_I739001 I739001] using standard cloning techniques. The lacI IS mutant presented derives from site-directed mutagenesis, which was performed on the precursor [http://partsregistry.org/wiki/index.php?title=Part:BBa_K142047 K142047] by PCR, subsequent DpnI digest and transformation. The following primers were used for mutagenesis: R197F forward CGGCGCGTCTGTTTCTGGCTGGCTG R197A forward CGGCGCGTCTGGCGCTGGCTGGCTG R197F reverse CAGCCAGCCAGAAACAGACGCGCCG R197A reverse CAGCCAGCCAGCGCCAGACGCGCCG T276F forward GGATACGACGATTTTGAAGACAGCTC T276A forward GGATACGACGATGCGGAAGACAGCTC T276F reverse GAGCTGTCTTCAAAATCGTCGTATCC T276A reverse GAGCTGTCTTCCGCATCGTCGTATCC
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_K142019
component: BBa_I739001
sequence annotation: BBa_I739001
sequence annotation: BBa_K142019
sequence: https://synbiohub.org/public/igem/BBa_K142027_sequence/1

BBa_K142019
type: Component
persistent identity: component2282804
display id: component2282804
version: 1
top level: BBa_K142027
access: public
definition: https://synbiohub.org/public/igem/BBa_K142019/1

BBa_I739001
type: Component
persistent identity: component2282819
display id: component2282819
version: 1
top level: BBa_K142027
access: public
definition: https://synbiohub.org/public/igem/BBa_I739001/1

BBa_I739001
type: SequenceAnnotation
persistent identity: annotation2282819
display id: annotation2282819
version: 1
top level: BBa_K142027
location: https://synbiohub.org/public/igem/BBa_K142027/annotation2282819/range2282819/1
component: BBa_I739001

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type: Range
persistent identity: range2282819
display id: range2282819
version: 1
direction: direction/reverse
top level: BBa_K142027
start: 1354
end: 2236
orientation: inline

BBa_K142019
type: SequenceAnnotation
persistent identity: annotation2282804
display id: annotation2282804
version: 1
top level: BBa_K142027
location: https://synbiohub.org/public/igem/BBa_K142027/annotation2282804/range2282804/1
component: BBa_K142019

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type: Range
persistent identity: range2282804
display id: range2282804
version: 1
direction: direction/reverse
top level: BBa_K142027
start: 1
end: 1345
orientation: inline

BBa_K1486005
type: ComponentDefinition
persistent identity: BBa K1486005
display id: BBa_K1486005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Arabinose promoter + sfGFP-CpxR [Cterm]
creator: iGem EPFL 2014
created: 2014-08-12T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part is composed of an arabinose-inducible promoter for superfolded GFP attached by a linker to CpxR (on the C terminus of CpxR).
mutable notes: We had to test both attachments, N and C terminus of the CpxR.
mutable provenance: The arabinose promoter and the sfGFP were obtained from the registry, while the CpxR was obtained from the E coli genome.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K1486003
component: BBa_K1486000
component: BBa_I746916
component: BBa_I0500
component: BBa_B0034
sequence annotation: BBa_K1486003
sequence annotation: BBa_B0034
sequence annotation: BBa_I746916
sequence annotation: BBa_I0500
sequence annotation: BBa_K1486000
sequence: https://synbiohub.org/public/igem/BBa_K1486005_sequence/1

BBa_K1486003
type: Component
persistent identity: component2382089
display id: component2382089
version: 1
top level: BBa_K1486005
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486003/1

BBa_K1486000
type: Component
persistent identity: component2382088
display id: component2382088
version: 1
top level: BBa_K1486005
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486000/1

BBa_I746916
type: Component
persistent identity: component2382093
display id: component2382093
version: 1
top level: BBa_K1486005
access: public
definition: https://synbiohub.org/public/igem/BBa_I746916/1

BBa_I0500
type: Component
persistent identity: component2382082
display id: component2382082
version: 1
top level: BBa_K1486005
access: public
definition: https://synbiohub.org/public/igem/BBa_I0500/1

BBa_B0034
type: Component
persistent identity: component2382084
display id: component2382084
version: 1
top level: BBa_K1486005
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K1486003
type: SequenceAnnotation
persistent identity: annotation2382089
display id: annotation2382089
version: 1
top level: BBa_K1486005
location: https://synbiohub.org/public/igem/BBa_K1486005/annotation2382089/range2382089/1
component: BBa_K1486003

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type: Range
persistent identity: range2382089
display id: range2382089
version: 1
direction: direction/reverse
top level: BBa_K1486005
start: 1925
end: 1948
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2382084
display id: annotation2382084
version: 1
top level: BBa_K1486005
location: https://synbiohub.org/public/igem/BBa_K1486005/annotation2382084/range2382084/1
component: BBa_B0034

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type: Range
persistent identity: range2382084
display id: range2382084
version: 1
direction: direction/reverse
top level: BBa_K1486005
start: 1211
end: 1222
orientation: inline

BBa_I746916
type: SequenceAnnotation
persistent identity: annotation2382093
display id: annotation2382093
version: 1
top level: BBa_K1486005
location: https://synbiohub.org/public/igem/BBa_K1486005/annotation2382093/range2382093/1
component: BBa_I746916

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type: Range
persistent identity: range2382093
display id: range2382093
version: 1
direction: direction/reverse
top level: BBa_K1486005
start: 1949
end: 2668
orientation: inline

BBa_I0500
type: SequenceAnnotation
persistent identity: annotation2382082
display id: annotation2382082
version: 1
top level: BBa_K1486005
location: https://synbiohub.org/public/igem/BBa_K1486005/annotation2382082/range2382082/1
component: BBa_I0500

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type: Range
persistent identity: range2382082
display id: range2382082
version: 1
direction: direction/reverse
top level: BBa_K1486005
start: 1
end: 1210
orientation: inline

BBa_K1486000
type: SequenceAnnotation
persistent identity: annotation2382088
display id: annotation2382088
version: 1
top level: BBa_K1486005
location: https://synbiohub.org/public/igem/BBa_K1486005/annotation2382088/range2382088/1
component: BBa_K1486000

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type: Range
persistent identity: range2382088
display id: range2382088
version: 1
direction: direction/reverse
top level: BBa_K1486005
start: 1223
end: 1924
orientation: inline

BBa_K783054
type: ComponentDefinition
persistent identity: BBa K783054
display id: BBa_K783054
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: This is a MoClo converted version of BBa_C0079
creator: Traci Haddock
created: 2012-10-01T11:00:00Z
modified: 2015-05-08T01:13:21Z
discontinued: false
dominant: false
group u list: _1038_
m user id: 0
owner id: 8248
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This is a MoClo converted version of BBa_C0079, which is the coding region for the lasR protein, which accepts chemical signal AI-1 (made by lasI protein). It has two MoClo fusion sites flanking it.
mutable notes: We had to design the proper MoClo fusion sites to flank this part.
mutable provenance: BBa_C0079
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: G
sequence annotation: lasR
sequence annotation: LVA
sequence: https://synbiohub.org/public/igem/BBa_K783054_sequence/1

G
type: SequenceAnnotation
persistent identity: annotation2214300
display id: annotation2214300
version: 1
top level: BBa_K783054
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K783054/annotation2214300/range2214300/1

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type: Range
persistent identity: range2214300
display id: range2214300
version: 1
direction: direction/forward
top level: BBa_K783054
start: 480
end: 480
orientation: inline

lasR
type: SequenceAnnotation
persistent identity: annotation2214299
display id: annotation2214299
version: 1
top level: BBa_K783054
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K783054/annotation2214299/range2214299/1

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type: Range
persistent identity: range2214299
display id: range2214299
version: 1
direction: direction/forward
top level: BBa_K783054
start: 1
end: 717
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation2214301
display id: annotation2214301
version: 1
top level: BBa_K783054
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K783054/annotation2214301/range2214301/1

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type: Range
persistent identity: range2214301
display id: range2214301
version: 1
direction: direction/forward
top level: BBa_K783054
start: 718
end: 750
orientation: inline

BBa_K283032
type: ComponentDefinition
persistent identity: BBa K283032
display id: BBa_K283032
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pTet-rbs-cI(lambda LVA)
creator: Nelson Chu, W Ling , Shi Lei
created: 2009-10-15T11:00:00Z
modified: 2015-05-08T01:11:47Z
discontinued: false
dominant: false
experience: experience/None
group u list: _383_
m user id: 0
owner id: 5494
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: THe pTet promoter can be repressed by TetR repressor protein. The cI protein generated can repress plambda(R-O12)(K094002) promoter. This part lacks a terminator
mutable notes: BBa_K283032
mutable provenance: The pTet promoter and the rbs comes from the registry, The cI protein comes from lambda phage
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_R0040
component: BBa_C0051
component: BBa_B0034
sequence annotation: BBa_R0040
sequence annotation: BBa_C0051
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K283032_sequence/1

BBa_R0040
type: Component
persistent identity: component2246756
display id: component2246756
version: 1
top level: BBa_K283032
access: public
definition: https://synbiohub.org/public/igem/BBa_R0040/1

BBa_C0051
type: Component
persistent identity: component2246766
display id: component2246766
version: 1
top level: BBa_K283032
access: public
definition: cI lam

BBa_B0034
type: Component
persistent identity: component2246762
display id: component2246762
version: 1
top level: BBa_K283032
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation2246756
display id: annotation2246756
version: 1
top level: BBa_K283032
location: https://synbiohub.org/public/igem/BBa_K283032/annotation2246756/range2246756/1
component: BBa_R0040

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type: Range
persistent identity: range2246756
display id: range2246756
version: 1
direction: direction/forward
top level: BBa_K283032
start: 1
end: 54
orientation: inline

BBa_C0051
type: SequenceAnnotation
persistent identity: annotation2246766
display id: annotation2246766
version: 1
top level: BBa_K283032
location: https://synbiohub.org/public/igem/BBa_K283032/annotation2246766/range2246766/1
component: BBa_C0051

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type: Range
persistent identity: range2246766
display id: range2246766
version: 1
direction: direction/reverse
top level: BBa_K283032
start: 81
end: 855
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2246762
display id: annotation2246762
version: 1
top level: BBa_K283032
location: https://synbiohub.org/public/igem/BBa_K283032/annotation2246762/range2246762/1
component: BBa_B0034

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type: Range
persistent identity: range2246762
display id: range2246762
version: 1
direction: direction/reverse
top level: BBa_K283032
start: 63
end: 74
orientation: inline

BBa_K165099
type: ComponentDefinition
persistent identity: BBa K165099
display id: BBa_K165099
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Gli1 bs + ADH1+ LexA repressor (CFPx2 tagged) on pRS304*
creator: John Szymanski
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:10:57Z
discontinued: false
dominant: false
group u list: _267_
m user id: 0
owner id: 2512
owning group id: 58
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: -
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_K165002
component: BBa_K165015
component: BBa_K165018
component: BBa_K165012
component: BBa_K165014
component: BBa_K165009
component: BBa_K165003
sequence annotation: BBa_K165009
sequence annotation: BBa_K165014
sequence annotation: BBa_K165002
sequence annotation: BBa_K165003
sequence annotation: BBa_K165018
sequence annotation: BBa_K165015
sequence annotation: BBa_K165012
sequence: https://synbiohub.org/public/igem/BBa_K165099_sequence/1

BBa_K165002
type: Component
persistent identity: component1997392
display id: component1997392
version: 1
top level: BBa_K165099
access: public
definition: https://synbiohub.org/public/igem/BBa_K165002/1

BBa_K165015
type: Component
persistent identity: component1997391
display id: component1997391
version: 1
top level: BBa_K165099
access: public
definition: https://synbiohub.org/public/igem/BBa_K165015/1

BBa_K165018
type: Component
persistent identity: component1997396
display id: component1997396
version: 1
top level: BBa_K165099
access: public
definition: https://synbiohub.org/public/igem/BBa_K165018/1

BBa_K165012
type: Component
persistent identity: component1997390
display id: component1997390
version: 1
top level: BBa_K165099
access: public
definition: https://synbiohub.org/public/igem/BBa_K165012/1

BBa_K165014
type: Component
persistent identity: component1997395
display id: component1997395
version: 1
top level: BBa_K165099
access: public
definition: https://synbiohub.org/public/igem/BBa_K165014/1

BBa_K165009
type: Component
persistent identity: component1997393
display id: component1997393
version: 1
top level: BBa_K165099
access: public
definition: https://synbiohub.org/public/igem/BBa_K165009/1

BBa_K165003
type: Component
persistent identity: component1997394
display id: component1997394
version: 1
top level: BBa_K165099
access: public
definition: https://synbiohub.org/public/igem/BBa_K165003/1

BBa_K165009
type: SequenceAnnotation
persistent identity: annotation1997393
display id: annotation1997393
version: 1
top level: BBa_K165099
location: https://synbiohub.org/public/igem/BBa_K165099/annotation1997393/range1997393/1
component: BBa_K165009

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type: Range
persistent identity: range1997393
display id: range1997393
version: 1
direction: direction/reverse
top level: BBa_K165099
start: 1615
end: 1872
orientation: inline

BBa_K165014
type: SequenceAnnotation
persistent identity: annotation1997395
display id: annotation1997395
version: 1
top level: BBa_K165099
location: https://synbiohub.org/public/igem/BBa_K165099/annotation1997395/range1997395/1
component: BBa_K165014

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type: Range
persistent identity: range1997395
display id: range1997395
version: 1
direction: direction/reverse
top level: BBa_K165099
start: 4700
end: 6193
orientation: inline

BBa_K165002
type: SequenceAnnotation
persistent identity: annotation1997392
display id: annotation1997392
version: 1
top level: BBa_K165099
location: https://synbiohub.org/public/igem/BBa_K165099/annotation1997392/range1997392/1
component: BBa_K165002

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type: Range
persistent identity: range1997392
display id: range1997392
version: 1
direction: direction/reverse
top level: BBa_K165099
start: 1589
end: 1606
orientation: inline

BBa_K165003
type: SequenceAnnotation
persistent identity: annotation1997394
display id: annotation1997394
version: 1
top level: BBa_K165099
location: https://synbiohub.org/public/igem/BBa_K165099/annotation1997394/range1997394/1
component: BBa_K165003

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type: Range
persistent identity: range1997394
display id: range1997394
version: 1
direction: direction/reverse
top level: BBa_K165099
start: 1881
end: 4691
orientation: inline

BBa_K165018
type: SequenceAnnotation
persistent identity: annotation1997396
display id: annotation1997396
version: 1
top level: BBa_K165099
location: https://synbiohub.org/public/igem/BBa_K165099/annotation1997396/range1997396/1
component: BBa_K165018

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type: Range
persistent identity: range1997396
display id: range1997396
version: 1
direction: direction/reverse
top level: BBa_K165099
start: 6202
end: 6454
orientation: inline

BBa_K165015
type: SequenceAnnotation
persistent identity: annotation1997391
display id: annotation1997391
version: 1
top level: BBa_K165099
location: https://synbiohub.org/public/igem/BBa_K165099/annotation1997391/range1997391/1
component: BBa_K165015

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type: Range
persistent identity: range1997391
display id: range1997391
version: 1
direction: direction/reverse
top level: BBa_K165099
start: 136
end: 1580
orientation: inline

BBa_K165012
type: SequenceAnnotation
persistent identity: annotation1997390
display id: annotation1997390
version: 1
top level: BBa_K165099
location: https://synbiohub.org/public/igem/BBa_K165099/annotation1997390/range1997390/1
component: BBa_K165012

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type: Range
persistent identity: range1997390
display id: range1997390
version: 1
direction: direction/reverse
top level: BBa_K165099
start: 1
end: 127
orientation: inline

c2 P22
type: ComponentDefinition
persistent identity: BBa C0053
display id: BBa_C0053
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: c2 repressor from Salmonella phage P22 (+LVA)
creator: Maia Mahoney
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:23Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: The P22 c2 repressor protein coding sequence is a 720 base-pair sequence with the standard RBS-compatible BioBrick prefix and the standard BioBrick suffix sections on its ends. It binds to the P22 c2 regulatory sequence, BBa_R0053. The sequence contains a LVA tag for faster degredation.
mutable notes: References (unparsed) here: Vander Byl,C. and Kropinski,A.M. Sequence of the genome of Salmonella bacteriophage P22 J. Bacteriol. 182 (22), 6472-6481 (2000) References (unparsed) here: Vander Byl,C. and Kropinski,A.M. Sequence of the genome of Salmonella bacteriophage P22 J. Bacteriol. 182 (22), 6472-6481 (2000)
mutable provenance: Bacteriophage P22
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Help:Barcodes
sequence annotation: stop
sequence annotation: LVA
sequence annotation: cII p22
sequence annotation: BBa_C0053
sequence: https://synbiohub.org/public/igem/BBa_C0053_sequence/1

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213993
display id: annotation2213993
version: 1
top level: c2 P22
role: SO:0000807
role: feature/barcode
location: https://synbiohub.org/public/igem/BBa_C0053/annotation2213993/range2213993/1

https://synbiohub.org/public/igem/BBa_C0053/annotation2213993/range2213993/1
type: Range
persistent identity: range2213993
display id: range2213993
version: 1
direction: direction/forward
top level: c2 P22
start: 688
end: 712
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1751
display id: annotation1751
version: 1
top level: c2 P22
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_C0053/annotation1751/range1751/1

https://synbiohub.org/public/igem/BBa_C0053/annotation1751/range1751/1
type: Range
persistent identity: range1751
display id: range1751
version: 1
direction: direction/forward
top level: c2 P22
start: 682
end: 687
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation1750
display id: annotation1750
version: 1
top level: c2 P22
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_C0053/annotation1750/range1750/1

https://synbiohub.org/public/igem/BBa_C0053/annotation1750/range1750/1
type: Range
persistent identity: range1750
display id: range1750
version: 1
direction: direction/forward
top level: c2 P22
start: 649
end: 681
orientation: inline

cII p22
type: SequenceAnnotation
persistent identity: annotation1747
display id: annotation1747
version: 1
top level: c2 P22
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_C0053/annotation1747/range1747/1

https://synbiohub.org/public/igem/BBa_C0053/annotation1747/range1747/1
type: Range
persistent identity: range1747
display id: range1747
version: 1
direction: direction/forward
top level: c2 P22
start: 1
end: 648
orientation: inline

BBa_C0053
type: SequenceAnnotation
persistent identity: annotation7036
display id: annotation7036
version: 1
top level: c2 P22
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_C0053/annotation7036/range7036/1

https://synbiohub.org/public/igem/BBa_C0053/annotation7036/range7036/1
type: Range
persistent identity: range7036
display id: range7036
version: 1
direction: direction/forward
top level: c2 P22
start: 1
end: 687
orientation: inline

BBa_K1520008
type: ComponentDefinition
persistent identity: BBa K1520008
display id: BBa_K1520008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Pcons2-rbs-lacI-Ter
creator: yue wu
created: 2014-09-21T11:00:00Z
modified: 2015-05-08T01:10:48Z
discontinued: false
dominant: false
group u list: _1902_
m user id: 0
owner id: 21318
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Pcons2-rbs-lacI-Ter. It constantly produces lacI.
mutable notes: None
mutable provenance: Pcons2, lacI, Ter
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_J23100
component: BBa_K1520029
component: BBa_K1520502
sequence annotation: BBa_J23100
sequence annotation: BBa_K1520029
sequence annotation: BBa_K1520502
sequence: https://synbiohub.org/public/igem/BBa_K1520008_sequence/1

BBa_J23100
type: Component
persistent identity: component2384932
display id: component2384932
version: 1
top level: BBa_K1520008
access: public
definition: https://synbiohub.org/public/igem/BBa_J23100/1

BBa_K1520029
type: Component
persistent identity: component2384936
display id: component2384936
version: 1
top level: BBa_K1520008
access: public
definition: https://synbiohub.org/public/igem/BBa_K1520029/1

BBa_K1520502
type: Component
persistent identity: component2384935
display id: component2384935
version: 1
top level: BBa_K1520008
access: public
definition: BBa_K1520502

BBa_J23100
type: SequenceAnnotation
persistent identity: annotation2384932
display id: annotation2384932
version: 1
top level: BBa_K1520008
location: https://synbiohub.org/public/igem/BBa_K1520008/annotation2384932/range2384932/1
component: BBa_J23100

https://synbiohub.org/public/igem/BBa_K1520008/annotation2384932/range2384932/1
type: Range
persistent identity: range2384932
display id: range2384932
version: 1
direction: direction/reverse
top level: BBa_K1520008
start: 1
end: 35
orientation: inline

BBa_K1520029
type: SequenceAnnotation
persistent identity: annotation2384936
display id: annotation2384936
version: 1
top level: BBa_K1520008
location: https://synbiohub.org/public/igem/BBa_K1520008/annotation2384936/range2384936/1
component: BBa_K1520029

https://synbiohub.org/public/igem/BBa_K1520008/annotation2384936/range2384936/1
type: Range
persistent identity: range2384936
display id: range2384936
version: 1
direction: direction/reverse
top level: BBa_K1520008
start: 1223
end: 1351
orientation: inline

BBa_K1520502
type: SequenceAnnotation
persistent identity: annotation2384935
display id: annotation2384935
version: 1
top level: BBa_K1520008
location: https://synbiohub.org/public/igem/BBa_K1520008/annotation2384935/range2384935/1
component: BBa_K1520502

https://synbiohub.org/public/igem/BBa_K1520008/annotation2384935/range2384935/1
type: Range
persistent identity: range2384935
display id: range2384935
version: 1
direction: direction/reverse
top level: BBa_K1520008
start: 44
end: 1214
orientation: inline

BBa_K1520501
type: ComponentDefinition
persistent identity: BBa K1520501
display id: BBa_K1520501
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Rbs-luxR(scarless)
creator: yue wu
created: 2014-09-21T11:00:00Z
modified: 2015-05-08T01:10:49Z
discontinued: false
dominant: false
group u list: _1902_
m user id: 0
owner id: 21318
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Rbs-luxR(scarless)
mutable notes: None
mutable provenance: None
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
sequence annotation: luxR
sequence annotation: rbs
sequence: https://synbiohub.org/public/igem/BBa_K1520501_sequence/1

luxR
type: SequenceAnnotation
persistent identity: annotation2384862
display id: annotation2384862
version: 1
top level: BBa_K1520501
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1520501/annotation2384862/range2384862/1

https://synbiohub.org/public/igem/BBa_K1520501/annotation2384862/range2384862/1
type: Range
persistent identity: range2384862
display id: range2384862
version: 1
direction: direction/forward
top level: BBa_K1520501
start: 19
end: 799
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation2384861
display id: annotation2384861
version: 1
top level: BBa_K1520501
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1520501/annotation2384861/range2384861/1

https://synbiohub.org/public/igem/BBa_K1520501/annotation2384861/range2384861/1
type: Range
persistent identity: range2384861
display id: range2384861
version: 1
direction: direction/forward
top level: BBa_K1520501
start: 1
end: 18
orientation: inline

BBa_K1682003
type: ComponentDefinition
persistent identity: BBa K1682003
display id: BBa_K1682003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PkdpF[-15, T>C]
creator: iGEM15_HKUST-Rice
created: 2015-08-09T11:00:00Z
modified: 2015-09-18T09:16:08Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2100_
m user id: 8758
owner id: 21235
owning group id: 9
status: status/Planning
bookmark: false
mutable description: kdpFp[-15, T>C]
mutable notes: kdpFp[-15, T>C]
mutable provenance: kdpFp[-15, T>C]
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -35
sequence annotation: phospho-KdpE binding site
sequence annotation: -10
sequence annotation: EcoRI Illegal site removed T->C
sequence: https://synbiohub.org/public/igem/BBa_K1682003_sequence/1

-35
type: SequenceAnnotation
persistent identity: annotation2473590
display id: annotation2473590
version: 1
top level: BBa_K1682003
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1682003/annotation2473590/range2473590/1

https://synbiohub.org/public/igem/BBa_K1682003/annotation2473590/range2473590/1
type: Range
persistent identity: range2473590
display id: range2473590
version: 1
direction: direction/forward
top level: BBa_K1682003
start: 42
end: 47
orientation: inline

phospho-KdpE binding site
type: SequenceAnnotation
persistent identity: annotation2473591
display id: annotation2473591
version: 1
top level: BBa_K1682003
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1682003/annotation2473591/range2473591/1

https://synbiohub.org/public/igem/BBa_K1682003/annotation2473591/range2473591/1
type: Range
persistent identity: range2473591
display id: range2473591
version: 1
direction: direction/forward
top level: BBa_K1682003
start: 8
end: 24
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2473589
display id: annotation2473589
version: 1
top level: BBa_K1682003
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1682003/annotation2473589/range2473589/1

https://synbiohub.org/public/igem/BBa_K1682003/annotation2473589/range2473589/1
type: Range
persistent identity: range2473589
display id: range2473589
version: 1
direction: direction/forward
top level: BBa_K1682003
start: 69
end: 74
orientation: inline

EcoRI Illegal site removed T->C
type: SequenceAnnotation
persistent identity: annotation2475214
display id: annotation2475214
version: 1
top level: BBa_K1682003
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K1682003/annotation2475214/range2475214/1

https://synbiohub.org/public/igem/BBa_K1682003/annotation2475214/range2475214/1
type: Range
persistent identity: range2475214
display id: range2475214
version: 1
direction: direction/forward
top level: BBa_K1682003
start: 65
end: 66
orientation: inline

BBa_P0352
type: ComponentDefinition
persistent identity: BBa P0352
display id: BBa_P0352
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PoPS -> cI (434) [S0160]
creator: Randy Rettberg
created: 2004-04-26T11:00:00Z
modified: 2015-05-08T01:14:10Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0033
component: BBa_C0052
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_C0052
sequence annotation: BBa_B0010
sequence annotation: BBa_B0033
sequence: https://synbiohub.org/public/igem/BBa_P0352_sequence/1

BBa_B0033
type: Component
persistent identity: component944754
display id: component944754
version: 1
top level: BBa_P0352
access: public
definition: https://synbiohub.org/public/igem/BBa_B0033/1

BBa_C0052
type: Component
persistent identity: component944765
display id: component944765
version: 1
top level: BBa_P0352
access: public
definition: cI 434

BBa_B0012
type: Component
persistent identity: component944782
display id: component944782
version: 1
top level: BBa_P0352
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0010
type: Component
persistent identity: component944772
display id: component944772
version: 1
top level: BBa_P0352
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation944782
display id: annotation944782
version: 1
top level: BBa_P0352
location: https://synbiohub.org/public/igem/BBa_P0352/annotation944782/range944782/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_P0352/annotation944782/range944782/1
type: Range
persistent identity: range944782
display id: range944782
version: 1
direction: direction/forward
top level: BBa_P0352
start: 808
end: 848
orientation: inline

BBa_C0052
type: SequenceAnnotation
persistent identity: annotation944765
display id: annotation944765
version: 1
top level: BBa_P0352
location: https://synbiohub.org/public/igem/BBa_P0352/annotation944765/range944765/1
component: BBa_C0052

https://synbiohub.org/public/igem/BBa_P0352/annotation944765/range944765/1
type: Range
persistent identity: range944765
display id: range944765
version: 1
direction: direction/forward
top level: BBa_P0352
start: 18
end: 686
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation944772
display id: annotation944772
version: 1
top level: BBa_P0352
location: https://synbiohub.org/public/igem/BBa_P0352/annotation944772/range944772/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_P0352/annotation944772/range944772/1
type: Range
persistent identity: range944772
display id: range944772
version: 1
direction: direction/forward
top level: BBa_P0352
start: 720
end: 799
orientation: inline

BBa_B0033
type: SequenceAnnotation
persistent identity: annotation944754
display id: annotation944754
version: 1
top level: BBa_P0352
location: https://synbiohub.org/public/igem/BBa_P0352/annotation944754/range944754/1
component: BBa_B0033

https://synbiohub.org/public/igem/BBa_P0352/annotation944754/range944754/1
type: Range
persistent identity: range944754
display id: range944754
version: 1
direction: direction/forward
top level: BBa_P0352
start: 1
end: 11
orientation: inline

BBa_K165096
type: ComponentDefinition
persistent identity: BBa K165096
display id: BBa_K165096
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Zif268-HIV bs + MET25+ Gli1 repressor (CFPx2 tagged) on pRS304*
creator: John Szymanski
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:10:57Z
discontinued: false
dominant: false
experience: experience/None
group u list: _267_
m user id: 0
owner id: 2512
owning group id: 58
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: -
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_K165014
component: BBa_K165000
component: BBa_K165018
component: BBa_K165002
component: BBa_K165003
component: BBa_K165011
component: BBa_K165007
sequence annotation: BBa_K165018
sequence annotation: BBa_K165007
sequence annotation: BBa_K165011
sequence annotation: BBa_K165003
sequence annotation: BBa_K165000
sequence annotation: BBa_K165014
sequence annotation: BBa_K165002
sequence: https://synbiohub.org/public/igem/BBa_K165096_sequence/1

BBa_K165014
type: Component
persistent identity: component1997365
display id: component1997365
version: 1
top level: BBa_K165096
access: public
definition: https://synbiohub.org/public/igem/BBa_K165014/1

BBa_K165000
type: Component
persistent identity: component1997361
display id: component1997361
version: 1
top level: BBa_K165096
access: public
definition: https://synbiohub.org/public/igem/BBa_K165000/1

BBa_K165018
type: Component
persistent identity: component1997366
display id: component1997366
version: 1
top level: BBa_K165096
access: public
definition: https://synbiohub.org/public/igem/BBa_K165018/1

BBa_K165002
type: Component
persistent identity: component1997362
display id: component1997362
version: 1
top level: BBa_K165096
access: public
definition: https://synbiohub.org/public/igem/BBa_K165002/1

BBa_K165003
type: Component
persistent identity: component1997363
display id: component1997363
version: 1
top level: BBa_K165096
access: public
definition: https://synbiohub.org/public/igem/BBa_K165003/1

BBa_K165011
type: Component
persistent identity: component1997360
display id: component1997360
version: 1
top level: BBa_K165096
access: public
definition: https://synbiohub.org/public/igem/BBa_K165011/1

BBa_K165007
type: Component
persistent identity: component1997364
display id: component1997364
version: 1
top level: BBa_K165096
access: public
definition: https://synbiohub.org/public/igem/BBa_K165007/1

BBa_K165018
type: SequenceAnnotation
persistent identity: annotation1997366
display id: annotation1997366
version: 1
top level: BBa_K165096
location: https://synbiohub.org/public/igem/BBa_K165096/annotation1997366/range1997366/1
component: BBa_K165018

https://synbiohub.org/public/igem/BBa_K165096/annotation1997366/range1997366/1
type: Range
persistent identity: range1997366
display id: range1997366
version: 1
direction: direction/reverse
top level: BBa_K165096
start: 5330
end: 5582
orientation: inline

BBa_K165007
type: SequenceAnnotation
persistent identity: annotation1997364
display id: annotation1997364
version: 1
top level: BBa_K165096
location: https://synbiohub.org/public/igem/BBa_K165096/annotation1997364/range1997364/1
component: BBa_K165007

https://synbiohub.org/public/igem/BBa_K165096/annotation1997364/range1997364/1
type: Range
persistent identity: range1997364
display id: range1997364
version: 1
direction: direction/reverse
top level: BBa_K165096
start: 3295
end: 3819
orientation: inline

BBa_K165011
type: SequenceAnnotation
persistent identity: annotation1997360
display id: annotation1997360
version: 1
top level: BBa_K165096
location: https://synbiohub.org/public/igem/BBa_K165096/annotation1997360/range1997360/1
component: BBa_K165011

https://synbiohub.org/public/igem/BBa_K165096/annotation1997360/range1997360/1
type: Range
persistent identity: range1997360
display id: range1997360
version: 1
direction: direction/reverse
top level: BBa_K165096
start: 1
end: 46
orientation: inline

BBa_K165003
type: SequenceAnnotation
persistent identity: annotation1997363
display id: annotation1997363
version: 1
top level: BBa_K165096
location: https://synbiohub.org/public/igem/BBa_K165096/annotation1997363/range1997363/1
component: BBa_K165003

https://synbiohub.org/public/igem/BBa_K165096/annotation1997363/range1997363/1
type: Range
persistent identity: range1997363
display id: range1997363
version: 1
direction: direction/reverse
top level: BBa_K165096
start: 476
end: 3286
orientation: inline

BBa_K165000
type: SequenceAnnotation
persistent identity: annotation1997361
display id: annotation1997361
version: 1
top level: BBa_K165096
location: https://synbiohub.org/public/igem/BBa_K165096/annotation1997361/range1997361/1
component: BBa_K165000

https://synbiohub.org/public/igem/BBa_K165096/annotation1997361/range1997361/1
type: Range
persistent identity: range1997361
display id: range1997361
version: 1
direction: direction/reverse
top level: BBa_K165096
start: 55
end: 441
orientation: inline

BBa_K165014
type: SequenceAnnotation
persistent identity: annotation1997365
display id: annotation1997365
version: 1
top level: BBa_K165096
location: https://synbiohub.org/public/igem/BBa_K165096/annotation1997365/range1997365/1
component: BBa_K165014

https://synbiohub.org/public/igem/BBa_K165096/annotation1997365/range1997365/1
type: Range
persistent identity: range1997365
display id: range1997365
version: 1
direction: direction/reverse
top level: BBa_K165096
start: 3828
end: 5321
orientation: inline

BBa_K165002
type: SequenceAnnotation
persistent identity: annotation1997362
display id: annotation1997362
version: 1
top level: BBa_K165096
location: https://synbiohub.org/public/igem/BBa_K165096/annotation1997362/range1997362/1
component: BBa_K165002

https://synbiohub.org/public/igem/BBa_K165096/annotation1997362/range1997362/1
type: Range
persistent identity: range1997362
display id: range1997362
version: 1
direction: direction/reverse
top level: BBa_K165096
start: 450
end: 467
orientation: inline

BBa_P0351
type: ComponentDefinition
persistent identity: BBa P0351
display id: BBa_P0351
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PoPS -> cI (lambda) [S0159]
creator: Randy Rettberg
created: 2004-04-26T11:00:00Z
modified: 2015-05-08T01:14:10Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0033
component: BBa_B0012
component: BBa_B0010
component: BBa_C0051
sequence annotation: BBa_C0051
sequence annotation: BBa_B0012
sequence annotation: BBa_B0033
sequence annotation: BBa_B0010
sequence: https://synbiohub.org/public/igem/BBa_P0351_sequence/1

BBa_B0033
type: Component
persistent identity: component944719
display id: component944719
version: 1
top level: BBa_P0351
access: public
definition: https://synbiohub.org/public/igem/BBa_B0033/1

BBa_B0012
type: Component
persistent identity: component944746
display id: component944746
version: 1
top level: BBa_P0351
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0010
type: Component
persistent identity: component944736
display id: component944736
version: 1
top level: BBa_P0351
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_C0051
type: Component
persistent identity: component944730
display id: component944730
version: 1
top level: BBa_P0351
access: public
definition: cI lam

BBa_C0051
type: SequenceAnnotation
persistent identity: annotation944730
display id: annotation944730
version: 1
top level: BBa_P0351
location: https://synbiohub.org/public/igem/BBa_P0351/annotation944730/range944730/1
component: BBa_C0051

https://synbiohub.org/public/igem/BBa_P0351/annotation944730/range944730/1
type: Range
persistent identity: range944730
display id: range944730
version: 1
direction: direction/forward
top level: BBa_P0351
start: 18
end: 767
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation944746
display id: annotation944746
version: 1
top level: BBa_P0351
location: https://synbiohub.org/public/igem/BBa_P0351/annotation944746/range944746/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_P0351/annotation944746/range944746/1
type: Range
persistent identity: range944746
display id: range944746
version: 1
direction: direction/forward
top level: BBa_P0351
start: 889
end: 929
orientation: inline

BBa_B0033
type: SequenceAnnotation
persistent identity: annotation944719
display id: annotation944719
version: 1
top level: BBa_P0351
location: https://synbiohub.org/public/igem/BBa_P0351/annotation944719/range944719/1
component: BBa_B0033

https://synbiohub.org/public/igem/BBa_P0351/annotation944719/range944719/1
type: Range
persistent identity: range944719
display id: range944719
version: 1
direction: direction/forward
top level: BBa_P0351
start: 1
end: 11
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation944736
display id: annotation944736
version: 1
top level: BBa_P0351
location: https://synbiohub.org/public/igem/BBa_P0351/annotation944736/range944736/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_P0351/annotation944736/range944736/1
type: Range
persistent identity: range944736
display id: range944736
version: 1
direction: direction/forward
top level: BBa_P0351
start: 801
end: 880
orientation: inline

BBa_K283048
type: ComponentDefinition
persistent identity: BBa K283048
display id: BBa_K283048
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: tetR(LVA) (Backbone: pSB1AK3)
creator: Shi Lei, W Ling , Nelson Chu
created: 2009-10-12T11:00:00Z
modified: 2015-05-08T01:11:48Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _383_
m user id: 0
owner id: 5494
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This part is adapted from K283006 with a terminator added to the end of the device.
mutable notes: BBa_K283007
mutable provenance: The CI, terminator, pLambda promoter and the chez coding region are self designed The rbs, and the pLux promoter are from the registry
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: SsrA
sequence annotation: BBa_C0040
sequence annotation: tetR
sequence annotation: barcode
sequence: https://synbiohub.org/public/igem/BBa_K283048_sequence/1

SsrA
type: SequenceAnnotation
persistent identity: annotation2057219
display id: annotation2057219
version: 1
top level: BBa_K283048
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K283048/annotation2057219/range2057219/1

https://synbiohub.org/public/igem/BBa_K283048/annotation2057219/range2057219/1
type: Range
persistent identity: range2057219
display id: range2057219
version: 1
direction: direction/forward
top level: BBa_K283048
start: 621
end: 654
orientation: inline

BBa_C0040
type: SequenceAnnotation
persistent identity: annotation2057220
display id: annotation2057220
version: 1
top level: BBa_K283048
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K283048/annotation2057220/range2057220/1

https://synbiohub.org/public/igem/BBa_K283048/annotation2057220/range2057220/1
type: Range
persistent identity: range2057220
display id: range2057220
version: 1
direction: direction/reverse
top level: BBa_K283048
start: 1
end: 660
orientation: inline

tetR
type: SequenceAnnotation
persistent identity: annotation2057218
display id: annotation2057218
version: 1
top level: BBa_K283048
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K283048/annotation2057218/range2057218/1

https://synbiohub.org/public/igem/BBa_K283048/annotation2057218/range2057218/1
type: Range
persistent identity: range2057218
display id: range2057218
version: 1
direction: direction/forward
top level: BBa_K283048
start: 4
end: 620
orientation: inline

barcode
type: SequenceAnnotation
persistent identity: annotation2057221
display id: annotation2057221
version: 1
top level: BBa_K283048
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K283048/annotation2057221/range2057221/1

https://synbiohub.org/public/igem/BBa_K283048/annotation2057221/range2057221/1
type: Range
persistent identity: range2057221
display id: range2057221
version: 1
direction: direction/reverse
top level: BBa_K283048
start: 661
end: 685
orientation: inline

BBa_K1480005
type: ComponentDefinition
persistent identity: BBa K1480005
display id: BBa_K1480005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TetR generator (Promoter J23111 )
creator: Jesus Gilberto Rodriguez Ceja
created: 2014-10-13T11:00:00Z
modified: 2015-05-08T01:10:40Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1860_
m user id: 0
owner id: 12878
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is a TetR protein generator regulated by the constitutive promoter J23111. The protein has a LVA tag for degradation.
mutable notes: It was assembled using assembly 10.
mutable provenance: Based on parts of the registry.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0034
component: BBa_C0040
component: BBa_B0012
component: BBa_J23111
component: BBa_B0010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0034
sequence annotation: BBa_J23111
sequence annotation: BBa_C0040
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_K1480005_sequence/1

BBa_B0034
type: Component
persistent identity: component2419556
display id: component2419556
version: 1
top level: BBa_K1480005
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_C0040
type: Component
persistent identity: component2419560
display id: component2419560
version: 1
top level: BBa_K1480005
access: public
definition: tetR

BBa_B0012
type: Component
persistent identity: component2419563
display id: component2419563
version: 1
top level: BBa_K1480005
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_J23111
type: Component
persistent identity: component2419554
display id: component2419554
version: 1
top level: BBa_K1480005
access: public
definition: https://synbiohub.org/public/igem/BBa_J23111/1

BBa_B0010
type: Component
persistent identity: component2419561
display id: component2419561
version: 1
top level: BBa_K1480005
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2419561
display id: annotation2419561
version: 1
top level: BBa_K1480005
location: https://synbiohub.org/public/igem/BBa_K1480005/annotation2419561/range2419561/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K1480005/annotation2419561/range2419561/1
type: Range
persistent identity: range2419561
display id: range2419561
version: 1
direction: direction/forward
top level: BBa_K1480005
start: 755
end: 834
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2419556
display id: annotation2419556
version: 1
top level: BBa_K1480005
location: https://synbiohub.org/public/igem/BBa_K1480005/annotation2419556/range2419556/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1480005/annotation2419556/range2419556/1
type: Range
persistent identity: range2419556
display id: range2419556
version: 1
direction: direction/reverse
top level: BBa_K1480005
start: 44
end: 55
orientation: inline

BBa_J23111
type: SequenceAnnotation
persistent identity: annotation2419554
display id: annotation2419554
version: 1
top level: BBa_K1480005
location: https://synbiohub.org/public/igem/BBa_K1480005/annotation2419554/range2419554/1
component: BBa_J23111

https://synbiohub.org/public/igem/BBa_K1480005/annotation2419554/range2419554/1
type: Range
persistent identity: range2419554
display id: range2419554
version: 1
direction: direction/reverse
top level: BBa_K1480005
start: 1
end: 35
orientation: inline

BBa_C0040
type: SequenceAnnotation
persistent identity: annotation2419560
display id: annotation2419560
version: 1
top level: BBa_K1480005
location: https://synbiohub.org/public/igem/BBa_K1480005/annotation2419560/range2419560/1
component: BBa_C0040

https://synbiohub.org/public/igem/BBa_K1480005/annotation2419560/range2419560/1
type: Range
persistent identity: range2419560
display id: range2419560
version: 1
direction: direction/reverse
top level: BBa_K1480005
start: 62
end: 746
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2419563
display id: annotation2419563
version: 1
top level: BBa_K1480005
location: https://synbiohub.org/public/igem/BBa_K1480005/annotation2419563/range2419563/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K1480005/annotation2419563/range2419563/1
type: Range
persistent identity: range2419563
display id: range2419563
version: 1
direction: direction/forward
top level: BBa_K1480005
start: 843
end: 883
orientation: inline

BBa_K1486056
type: ComponentDefinition
persistent identity: BBa K1486056
display id: BBa_K1486056
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CxpR & Split IFP1.4 [Cterm + Cterm][2]
creator: iGem EPFL 2014
created: 2014-10-06T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This part is the twin of the non-compatible biobrick BBa_K1486008 rendered compatible by mutating the two illegal restriction sites. However, all experiments were run on the aforementioned biobrick.
mutable notes: Site-directed mutagenesis was used to remove the restriction sites in the sequence.
mutable provenance: http://www.addgene.org/browse/article/8177/ which was then mutated by us.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_K1486004
component: BBa_K1486000
component: BBa_B0034
component: BBa_I0500
component: BBa_K1486044
component: BBa_K1486045
sequence annotation: BBa_K1486000
sequence annotation: BBa_K1486004
sequence annotation: BBa_B0034
sequence annotation: BBa_I0500
sequence annotation: BBa_K1486044
sequence annotation: BBa_K1486045
sequence: https://synbiohub.org/public/igem/BBa_K1486056_sequence/1

BBa_K1486004
type: Component
persistent identity: component2398606
display id: component2398606
version: 1
top level: BBa_K1486056
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486004/1

BBa_K1486000
type: Component
persistent identity: component2398605
display id: component2398605
version: 1
top level: BBa_K1486056
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486000/1

BBa_K1486004
type: Component
persistent identity: component2398614
display id: component2398614
version: 1
top level: BBa_K1486056
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486004/1

BBa_K1486000
type: Component
persistent identity: component2398613
display id: component2398613
version: 1
top level: BBa_K1486056
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486000/1

BBa_B0034
type: Component
persistent identity: component2398609
display id: component2398609
version: 1
top level: BBa_K1486056
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_I0500
type: Component
persistent identity: component2398599
display id: component2398599
version: 1
top level: BBa_K1486056
access: public
definition: https://synbiohub.org/public/igem/BBa_I0500/1

BBa_K1486044
type: Component
persistent identity: component2398607
display id: component2398607
version: 1
top level: BBa_K1486056
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486044/1

BBa_B0034
type: Component
persistent identity: component2398601
display id: component2398601
version: 1
top level: BBa_K1486056
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K1486045
type: Component
persistent identity: component2398615
display id: component2398615
version: 1
top level: BBa_K1486056
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486045/1

BBa_K1486000
type: SequenceAnnotation
persistent identity: annotation2398613
display id: annotation2398613
version: 1
top level: BBa_K1486056
location: https://synbiohub.org/public/igem/BBa_K1486056/annotation2398613/range2398613/1
component: BBa_K1486000

https://synbiohub.org/public/igem/BBa_K1486056/annotation2398613/range2398613/1
type: Range
persistent identity: range2398613
display id: range2398613
version: 1
direction: direction/reverse
top level: BBa_K1486056
start: 2413
end: 3114
orientation: inline

BBa_K1486004
type: SequenceAnnotation
persistent identity: annotation2398606
display id: annotation2398606
version: 1
top level: BBa_K1486056
location: https://synbiohub.org/public/igem/BBa_K1486056/annotation2398606/range2398606/1
component: BBa_K1486004

https://synbiohub.org/public/igem/BBa_K1486056/annotation2398606/range2398606/1
type: Range
persistent identity: range2398606
display id: range2398606
version: 1
direction: direction/reverse
top level: BBa_K1486056
start: 1947
end: 1976
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2398601
display id: annotation2398601
version: 1
top level: BBa_K1486056
location: https://synbiohub.org/public/igem/BBa_K1486056/annotation2398601/range2398601/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1486056/annotation2398601/range2398601/1
type: Range
persistent identity: range2398601
display id: range2398601
version: 1
direction: direction/reverse
top level: BBa_K1486056
start: 1219
end: 1230
orientation: inline

BBa_K1486000
type: SequenceAnnotation
persistent identity: annotation2398605
display id: annotation2398605
version: 1
top level: BBa_K1486056
location: https://synbiohub.org/public/igem/BBa_K1486056/annotation2398605/range2398605/1
component: BBa_K1486000

https://synbiohub.org/public/igem/BBa_K1486056/annotation2398605/range2398605/1
type: Range
persistent identity: range2398605
display id: range2398605
version: 1
direction: direction/reverse
top level: BBa_K1486056
start: 1237
end: 1938
orientation: inline

BBa_I0500
type: SequenceAnnotation
persistent identity: annotation2398599
display id: annotation2398599
version: 1
top level: BBa_K1486056
location: https://synbiohub.org/public/igem/BBa_K1486056/annotation2398599/range2398599/1
component: BBa_I0500

https://synbiohub.org/public/igem/BBa_K1486056/annotation2398599/range2398599/1
type: Range
persistent identity: range2398599
display id: range2398599
version: 1
direction: direction/reverse
top level: BBa_K1486056
start: 1
end: 1210
orientation: inline

BBa_K1486044
type: SequenceAnnotation
persistent identity: annotation2398607
display id: annotation2398607
version: 1
top level: BBa_K1486056
location: https://synbiohub.org/public/igem/BBa_K1486056/annotation2398607/range2398607/1
component: BBa_K1486044

https://synbiohub.org/public/igem/BBa_K1486056/annotation2398607/range2398607/1
type: Range
persistent identity: range2398607
display id: range2398607
version: 1
direction: direction/reverse
top level: BBa_K1486056
start: 1985
end: 2386
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2398609
display id: annotation2398609
version: 1
top level: BBa_K1486056
location: https://synbiohub.org/public/igem/BBa_K1486056/annotation2398609/range2398609/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1486056/annotation2398609/range2398609/1
type: Range
persistent identity: range2398609
display id: range2398609
version: 1
direction: direction/reverse
top level: BBa_K1486056
start: 2395
end: 2406
orientation: inline

BBa_K1486004
type: SequenceAnnotation
persistent identity: annotation2398614
display id: annotation2398614
version: 1
top level: BBa_K1486056
location: https://synbiohub.org/public/igem/BBa_K1486056/annotation2398614/range2398614/1
component: BBa_K1486004

https://synbiohub.org/public/igem/BBa_K1486056/annotation2398614/range2398614/1
type: Range
persistent identity: range2398614
display id: range2398614
version: 1
direction: direction/reverse
top level: BBa_K1486056
start: 3123
end: 3152
orientation: inline

BBa_K1486045
type: SequenceAnnotation
persistent identity: annotation2398615
display id: annotation2398615
version: 1
top level: BBa_K1486056
location: https://synbiohub.org/public/igem/BBa_K1486056/annotation2398615/range2398615/1
component: BBa_K1486045

https://synbiohub.org/public/igem/BBa_K1486056/annotation2398615/range2398615/1
type: Range
persistent identity: range2398615
display id: range2398615
version: 1
direction: direction/reverse
top level: BBa_K1486056
start: 3161
end: 3742
orientation: inline

lasR
type: ComponentDefinition
persistent identity: BBa C0179
display id: BBa_C0179
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: lasR activator from P. aeruginosa PAO1(no LVA)
creator: jcbraff
created: 2004-05-26T11:00:00Z
modified: 2015-08-31T04:07:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_1_
m user id: 0
owner id: 61
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: same as C0079 except no LVA tag
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_C0179_sequence/1

BBa_P0350
type: ComponentDefinition
persistent identity: BBa P0350
display id: BBa_P0350
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PoPS -> cI (HK022) [S0158]
creator: Randy Rettberg
created: 2004-04-26T11:00:00Z
modified: 2015-05-08T01:14:10Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0010
component: BBa_C0050
component: BBa_B0033
component: BBa_B0012
sequence annotation: BBa_B0033
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_C0050
sequence: https://synbiohub.org/public/igem/BBa_P0350_sequence/1

BBa_B0010
type: Component
persistent identity: component944701
display id: component944701
version: 1
top level: BBa_P0350
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_C0050
type: Component
persistent identity: component944693
display id: component944693
version: 1
top level: BBa_P0350
access: public
definition: cI HK022

BBa_B0033
type: Component
persistent identity: component944677
display id: component944677
version: 1
top level: BBa_P0350
access: public
definition: https://synbiohub.org/public/igem/BBa_B0033/1

BBa_B0012
type: Component
persistent identity: component944711
display id: component944711
version: 1
top level: BBa_P0350
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0033
type: SequenceAnnotation
persistent identity: annotation944677
display id: annotation944677
version: 1
top level: BBa_P0350
location: https://synbiohub.org/public/igem/BBa_P0350/annotation944677/range944677/1
component: BBa_B0033

https://synbiohub.org/public/igem/BBa_P0350/annotation944677/range944677/1
type: Range
persistent identity: range944677
display id: range944677
version: 1
direction: direction/forward
top level: BBa_P0350
start: 1
end: 11
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation944701
display id: annotation944701
version: 1
top level: BBa_P0350
location: https://synbiohub.org/public/igem/BBa_P0350/annotation944701/range944701/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_P0350/annotation944701/range944701/1
type: Range
persistent identity: range944701
display id: range944701
version: 1
direction: direction/forward
top level: BBa_P0350
start: 795
end: 874
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation944711
display id: annotation944711
version: 1
top level: BBa_P0350
location: https://synbiohub.org/public/igem/BBa_P0350/annotation944711/range944711/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_P0350/annotation944711/range944711/1
type: Range
persistent identity: range944711
display id: range944711
version: 1
direction: direction/forward
top level: BBa_P0350
start: 883
end: 923
orientation: inline

BBa_C0050
type: SequenceAnnotation
persistent identity: annotation944693
display id: annotation944693
version: 1
top level: BBa_P0350
location: https://synbiohub.org/public/igem/BBa_P0350/annotation944693/range944693/1
component: BBa_C0050

https://synbiohub.org/public/igem/BBa_P0350/annotation944693/range944693/1
type: Range
persistent identity: range944693
display id: range944693
version: 1
direction: direction/forward
top level: BBa_P0350
start: 18
end: 761
orientation: inline

BBa_K822004
type: ComponentDefinition
persistent identity: BBa K822004
display id: BBa_K822004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LacI generator (no promoter)
creator: Jarle Pahr
created: 2012-09-24T11:00:00Z
modified: 2015-05-08T01:13:29Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1080_
m user id: 0
owner id: 11627
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: A composite part with coding sequence for LacI-LVA together with ribosome binding site (RBS) and a double transcriptional terminator. Fore use in regulation of LacI-repressed genetic elements, by combining the part with an appropriate promoter.
mutable notes: This part is a reconstruction of BBa_K292006. BBa_K292006 has the same theoretical sequence, but the DNA available through the registry does not match this sequence. We thus offer this part as a replacement.
mutable provenance: BioBrick parts from the Registry.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0030
component: BBa_C0012
component: BBa_B0014
sequence annotation: BBa_C0012
sequence annotation: BBa_B0014
sequence annotation: BBa_B0030
sequence: https://synbiohub.org/public/igem/BBa_K822004_sequence/1

BBa_B0030
type: Component
persistent identity: component2251178
display id: component2251178
version: 1
top level: BBa_K822004
access: public
definition: https://synbiohub.org/public/igem/BBa_B0030/1

BBa_C0012
type: Component
persistent identity: component2251181
display id: component2251181
version: 1
top level: BBa_K822004
access: public
definition: lacI

BBa_B0014
type: Component
persistent identity: component2251190
display id: component2251190
version: 1
top level: BBa_K822004
access: public
definition: https://synbiohub.org/public/igem/BBa_B0014/1

BBa_C0012
type: SequenceAnnotation
persistent identity: annotation2251181
display id: annotation2251181
version: 1
top level: BBa_K822004
location: https://synbiohub.org/public/igem/BBa_K822004/annotation2251181/range2251181/1
component: BBa_C0012

https://synbiohub.org/public/igem/BBa_K822004/annotation2251181/range2251181/1
type: Range
persistent identity: range2251181
display id: range2251181
version: 1
direction: direction/forward
top level: BBa_K822004
start: 22
end: 1149
orientation: inline

BBa_B0014
type: SequenceAnnotation
persistent identity: annotation2251190
display id: annotation2251190
version: 1
top level: BBa_K822004
location: https://synbiohub.org/public/igem/BBa_K822004/annotation2251190/range2251190/1
component: BBa_B0014

https://synbiohub.org/public/igem/BBa_K822004/annotation2251190/range2251190/1
type: Range
persistent identity: range2251190
display id: range2251190
version: 1
direction: direction/reverse
top level: BBa_K822004
start: 1183
end: 1277
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation2251178
display id: annotation2251178
version: 1
top level: BBa_K822004
location: https://synbiohub.org/public/igem/BBa_K822004/annotation2251178/range2251178/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_K822004/annotation2251178/range2251178/1
type: Range
persistent identity: range2251178
display id: range2251178
version: 1
direction: direction/forward
top level: BBa_K822004
start: 1
end: 15
orientation: inline

BBa_K1486012
type: ComponentDefinition
persistent identity: BBa K1486012
display id: BBa_K1486012
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CpxR-IFP[1] under pBAD
creator: iGem EPFL 2014
created: 2014-08-31T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part has a fused protein downstream of an arabinose-inducible promoter: CpxR-IFP[1]. Biobrick BBa_K1486013 has CpxR-IFP[2] with an arabinose promoter. This construct was made in both orientations: BBa_K1486014 is IFP[1]-CpxR (with an arabinose promoter).
mutable notes: Both orientations were made, as well as for IFP[2] so all dimerizations could be tested (see main page for biobrick numbers). Flexible linkers were used to fuse both proteins, and an RBS was put before each one of them.
mutable provenance: To Edit
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K1486006
component: BBa_K1486004
component: BBa_K1486000
component: BBa_I0500
component: BBa_B0034
sequence annotation: BBa_K1486006
sequence annotation: BBa_B0034
sequence annotation: BBa_K1486000
sequence annotation: BBa_K1486004
sequence annotation: BBa_I0500
sequence: https://synbiohub.org/public/igem/BBa_K1486012_sequence/1

BBa_K1486006
type: Component
persistent identity: component2382293
display id: component2382293
version: 1
top level: BBa_K1486012
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486006/1

BBa_K1486004
type: Component
persistent identity: component2382292
display id: component2382292
version: 1
top level: BBa_K1486012
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486004/1

BBa_K1486000
type: Component
persistent identity: component2382291
display id: component2382291
version: 1
top level: BBa_K1486012
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486000/1

BBa_I0500
type: Component
persistent identity: component2382285
display id: component2382285
version: 1
top level: BBa_K1486012
access: public
definition: https://synbiohub.org/public/igem/BBa_I0500/1

BBa_B0034
type: Component
persistent identity: component2382287
display id: component2382287
version: 1
top level: BBa_K1486012
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K1486006
type: SequenceAnnotation
persistent identity: annotation2382293
display id: annotation2382293
version: 1
top level: BBa_K1486012
location: https://synbiohub.org/public/igem/BBa_K1486012/annotation2382293/range2382293/1
component: BBa_K1486006

https://synbiohub.org/public/igem/BBa_K1486012/annotation2382293/range2382293/1
type: Range
persistent identity: range2382293
display id: range2382293
version: 1
direction: direction/reverse
top level: BBa_K1486012
start: 1955
end: 2536
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2382287
display id: annotation2382287
version: 1
top level: BBa_K1486012
location: https://synbiohub.org/public/igem/BBa_K1486012/annotation2382287/range2382287/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1486012/annotation2382287/range2382287/1
type: Range
persistent identity: range2382287
display id: range2382287
version: 1
direction: direction/reverse
top level: BBa_K1486012
start: 1211
end: 1222
orientation: inline

BBa_K1486000
type: SequenceAnnotation
persistent identity: annotation2382291
display id: annotation2382291
version: 1
top level: BBa_K1486012
location: https://synbiohub.org/public/igem/BBa_K1486012/annotation2382291/range2382291/1
component: BBa_K1486000

https://synbiohub.org/public/igem/BBa_K1486012/annotation2382291/range2382291/1
type: Range
persistent identity: range2382291
display id: range2382291
version: 1
direction: direction/reverse
top level: BBa_K1486012
start: 1223
end: 1924
orientation: inline

BBa_K1486004
type: SequenceAnnotation
persistent identity: annotation2382292
display id: annotation2382292
version: 1
top level: BBa_K1486012
location: https://synbiohub.org/public/igem/BBa_K1486012/annotation2382292/range2382292/1
component: BBa_K1486004

https://synbiohub.org/public/igem/BBa_K1486012/annotation2382292/range2382292/1
type: Range
persistent identity: range2382292
display id: range2382292
version: 1
direction: direction/reverse
top level: BBa_K1486012
start: 1925
end: 1954
orientation: inline

BBa_I0500
type: SequenceAnnotation
persistent identity: annotation2382285
display id: annotation2382285
version: 1
top level: BBa_K1486012
location: https://synbiohub.org/public/igem/BBa_K1486012/annotation2382285/range2382285/1
component: BBa_I0500

https://synbiohub.org/public/igem/BBa_K1486012/annotation2382285/range2382285/1
type: Range
persistent identity: range2382285
display id: range2382285
version: 1
direction: direction/reverse
top level: BBa_K1486012
start: 1
end: 1210
orientation: inline

pBAD:CspA
type: ComponentDefinition
persistent identity: BBa K538303
display id: BBa_K538303
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pBAD + RBS + CspA
creator: Bas Stringer
created: 2011-09-07T11:00:00Z
modified: 2015-05-08T01:12:37Z
discontinued: false
dominant: false
experience: experience/None
group u list: _704_
m user id: 0
owner id: 9985
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: -
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K206000
component: BBa_K538003
component: BBa_B0034
sequence annotation: BBa_B0034
sequence annotation: BBa_K206000
sequence annotation: BBa_K538003
sequence: https://synbiohub.org/public/igem/BBa_K538303_sequence/1

BBa_K206000
type: Component
persistent identity: component2134090
display id: component2134090
version: 1
top level: pBAD:CspA
access: public
definition: https://synbiohub.org/public/igem/BBa_K206000/1

BBa_K538003
type: Component
persistent identity: component2134097
display id: component2134097
version: 1
top level: pBAD:CspA
access: public
definition: CspA

BBa_B0034
type: Component
persistent identity: component2134092
display id: component2134092
version: 1
top level: pBAD:CspA
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2134092
display id: annotation2134092
version: 1
top level: pBAD:CspA
location: https://synbiohub.org/public/igem/BBa_K538303/annotation2134092/range2134092/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K538303/annotation2134092/range2134092/1
type: Range
persistent identity: range2134092
display id: range2134092
version: 1
direction: direction/reverse
top level: pBAD:CspA
start: 139
end: 150
orientation: inline

BBa_K206000
type: SequenceAnnotation
persistent identity: annotation2134090
display id: annotation2134090
version: 1
top level: pBAD:CspA
location: https://synbiohub.org/public/igem/BBa_K538303/annotation2134090/range2134090/1
component: BBa_K206000

https://synbiohub.org/public/igem/BBa_K538303/annotation2134090/range2134090/1
type: Range
persistent identity: range2134090
display id: range2134090
version: 1
direction: direction/reverse
top level: pBAD:CspA
start: 1
end: 130
orientation: inline

BBa_K538003
type: SequenceAnnotation
persistent identity: annotation2134097
display id: annotation2134097
version: 1
top level: pBAD:CspA
location: https://synbiohub.org/public/igem/BBa_K538303/annotation2134097/range2134097/1
component: BBa_K538003

https://synbiohub.org/public/igem/BBa_K538303/annotation2134097/range2134097/1
type: Range
persistent identity: range2134097
display id: range2134097
version: 1
direction: direction/reverse
top level: pBAD:CspA
start: 157
end: 351
orientation: inline

BBa_P0112
type: ComponentDefinition
persistent identity: BBa P0112
display id: BBa_P0112
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PoPS -> LacI [S0162]
creator: Randy Rettberg
created: 2004-04-24T11:00:00Z
modified: 2015-05-08T01:14:10Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Protein generator converting TIPS to the protein LacI. Used as the input section for Quad Part Inverters Q01121 and Q01122.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_C0012
component: BBa_B0031
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_B0010
sequence annotation: BBa_B0031
sequence annotation: BBa_C0012
sequence: https://synbiohub.org/public/igem/BBa_P0112_sequence/1

BBa_C0012
type: Component
persistent identity: component944409
display id: component944409
version: 1
top level: BBa_P0112
access: public
definition: lacI

BBa_B0031
type: Component
persistent identity: component944399
display id: component944399
version: 1
top level: BBa_P0112
access: public
definition: https://synbiohub.org/public/igem/BBa_B0031/1

BBa_B0012
type: Component
persistent identity: component944426
display id: component944426
version: 1
top level: BBa_P0112
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0010
type: Component
persistent identity: component944416
display id: component944416
version: 1
top level: BBa_P0112
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation944426
display id: annotation944426
version: 1
top level: BBa_P0112
location: https://synbiohub.org/public/igem/BBa_P0112/annotation944426/range944426/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_P0112/annotation944426/range944426/1
type: Range
persistent identity: range944426
display id: range944426
version: 1
direction: direction/forward
top level: BBa_P0112
start: 1270
end: 1310
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation944416
display id: annotation944416
version: 1
top level: BBa_P0112
location: https://synbiohub.org/public/igem/BBa_P0112/annotation944416/range944416/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_P0112/annotation944416/range944416/1
type: Range
persistent identity: range944416
display id: range944416
version: 1
direction: direction/forward
top level: BBa_P0112
start: 1182
end: 1261
orientation: inline

BBa_B0031
type: SequenceAnnotation
persistent identity: annotation944399
display id: annotation944399
version: 1
top level: BBa_P0112
location: https://synbiohub.org/public/igem/BBa_P0112/annotation944399/range944399/1
component: BBa_B0031

https://synbiohub.org/public/igem/BBa_P0112/annotation944399/range944399/1
type: Range
persistent identity: range944399
display id: range944399
version: 1
direction: direction/forward
top level: BBa_P0112
start: 1
end: 14
orientation: inline

BBa_C0012
type: SequenceAnnotation
persistent identity: annotation944409
display id: annotation944409
version: 1
top level: BBa_P0112
location: https://synbiohub.org/public/igem/BBa_P0112/annotation944409/range944409/1
component: BBa_C0012

https://synbiohub.org/public/igem/BBa_P0112/annotation944409/range944409/1
type: Range
persistent identity: range944409
display id: range944409
version: 1
direction: direction/forward
top level: BBa_P0112
start: 21
end: 1148
orientation: inline

cI lam
type: ComponentDefinition
persistent identity: BBa C0051
display id: BBa_C0051
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: cI repressor from E. coli phage lambda (+LVA)
creator: Vinay S Mahajan, Brian Chow, Peter Carr, Voichita Marinescu and Alexander D. Wissner-Gross
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:23Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Coding region for the cI repressor based on cI repressor from bacteriophage lambda modified with an LVA tail for rapid degradation of the protein. cI repressor binds to the cI regulator (BBa_R0051).
mutable notes: References (unparsed) here: A synthetic oscillatory network of transcriptional regulators , Elowitz M.B. , Leibler S., Nature(403),335-38: 2000 Octamerization of CI repressor is needed for effective repression of PRM and efficient switching from lysogeny. Ian B. Dodd,1 Alison J. Perkins, Daniel Tsemitsidis, and J. Barry Egan , Genes and Development (Vol 15, No. 22) 3013-3022: 2001 References (unparsed) here: A synthetic oscillatory network of transcriptional regulators , Elowitz M.B. , Leibler S., Nature(403),335-38: 2000 Octamerization of CI repressor is needed for effective repression of PRM and efficient switching from lysogeny. Ian B. Dodd,1 Alison J. Perkins, Daniel Tsemitsidis, and J. Barry Egan , Genes and Development (Vol 15, No. 22) 3013-3022: 2001 BBa_C0051 cI repressor is based on the cI repressor from the Elowitz's repressilator. It has been modified to include a rapid degradation LAA tail, and includes the BioBrick standard assembly head and tail restriction sites. The RBS has been removed. The stop codon has been changed from TAA to a double stop codon TAATAA.
mutable provenance: Elowitz, M. B. Transport, Assembly, and Dynamics in Systems of Interacting Proteins. Thesis, Princeton Univ., Princeton (1999).
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: LVA
sequence annotation: cI lambda
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C0051_sequence/1

LVA
type: SequenceAnnotation
persistent identity: annotation23335
display id: annotation23335
version: 1
top level: cI lam
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_C0051/annotation23335/range23335/1

https://synbiohub.org/public/igem/BBa_C0051/annotation23335/range23335/1
type: Range
persistent identity: range23335
display id: range23335
version: 1
direction: direction/forward
top level: cI lam
start: 712
end: 744
orientation: inline

cI lambda
type: SequenceAnnotation
persistent identity: annotation23334
display id: annotation23334
version: 1
top level: cI lam
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_C0051/annotation23334/range23334/1

https://synbiohub.org/public/igem/BBa_C0051/annotation23334/range23334/1
type: Range
persistent identity: range23334
display id: range23334
version: 1
direction: direction/forward
top level: cI lam
start: 4
end: 711
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213991
display id: annotation2213991
version: 1
top level: cI lam
role: SO:0000807
role: feature/barcode
location: https://synbiohub.org/public/igem/BBa_C0051/annotation2213991/range2213991/1

https://synbiohub.org/public/igem/BBa_C0051/annotation2213991/range2213991/1
type: Range
persistent identity: range2213991
display id: range2213991
version: 1
direction: direction/forward
top level: cI lam
start: 751
end: 775
orientation: inline

BBa_K1520518
type: ComponentDefinition
persistent identity: BBa K1520518
display id: BBa_K1520518
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PgolTS-golS-PgolB-tetR-Ter
creator: yue wu
created: 2014-09-22T11:00:00Z
modified: 2015-05-08T01:10:49Z
discontinued: false
dominant: false
group u list: _1902_
m user id: 0
owner id: 21318
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: PgolTS-golS-PgolB-tetR-Ter If there exist gold ions, tetR will be produced.
mutable notes: None
mutable provenance: PgolTS-golS-PgolB, tetR, Ter
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1520029
component: BBa_K1520506
component: BBa_K1520505
sequence annotation: BBa_K1520505
sequence annotation: BBa_K1520506
sequence annotation: BBa_K1520029
sequence: https://synbiohub.org/public/igem/BBa_K1520518_sequence/1

BBa_K1520029
type: Component
persistent identity: component2385743
display id: component2385743
version: 1
top level: BBa_K1520518
access: public
definition: https://synbiohub.org/public/igem/BBa_K1520029/1

BBa_K1520506
type: Component
persistent identity: component2385739
display id: component2385739
version: 1
top level: BBa_K1520518
access: public
definition: https://synbiohub.org/public/igem/BBa_K1520506/1

BBa_K1520505
type: Component
persistent identity: component2385742
display id: component2385742
version: 1
top level: BBa_K1520518
access: public
definition: BBa_K1520505

BBa_K1520505
type: SequenceAnnotation
persistent identity: annotation2385742
display id: annotation2385742
version: 1
top level: BBa_K1520518
location: https://synbiohub.org/public/igem/BBa_K1520518/annotation2385742/range2385742/1
component: BBa_K1520505

https://synbiohub.org/public/igem/BBa_K1520518/annotation2385742/range2385742/1
type: Range
persistent identity: range2385742
display id: range2385742
version: 1
direction: direction/reverse
top level: BBa_K1520518
start: 762
end: 1464
orientation: inline

BBa_K1520506
type: SequenceAnnotation
persistent identity: annotation2385739
display id: annotation2385739
version: 1
top level: BBa_K1520518
location: https://synbiohub.org/public/igem/BBa_K1520518/annotation2385739/range2385739/1
component: BBa_K1520506

https://synbiohub.org/public/igem/BBa_K1520518/annotation2385739/range2385739/1
type: Range
persistent identity: range2385739
display id: range2385739
version: 1
direction: direction/reverse
top level: BBa_K1520518
start: 1
end: 753
orientation: inline

BBa_K1520029
type: SequenceAnnotation
persistent identity: annotation2385743
display id: annotation2385743
version: 1
top level: BBa_K1520518
location: https://synbiohub.org/public/igem/BBa_K1520518/annotation2385743/range2385743/1
component: BBa_K1520029

https://synbiohub.org/public/igem/BBa_K1520518/annotation2385743/range2385743/1
type: Range
persistent identity: range2385743
display id: range2385743
version: 1
direction: direction/reverse
top level: BBa_K1520518
start: 1473
end: 1601
orientation: inline

BBa_K1031019
type: ComponentDefinition
persistent identity: BBa K1031019
display id: BBa_K1031019
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: NahR-Psal-B0034-RiboJ-φR73δ
creator: He Shuaixin
created: 2013-10-21T11:00:00Z
modified: 2015-05-08T01:08:47Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1338_
m user id: 0
owner id: 15843
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: NahR-Psal-φR73Δ
mutable notes: molecular cloning
mutable provenance: φR73; Pseudomonas. putida
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: NahR
sequence annotation: phiR73delta
sequence annotation: Pr
sequence annotation: Psal
sequence: https://synbiohub.org/public/igem/BBa_K1031019_sequence/1

NahR
type: SequenceAnnotation
persistent identity: annotation2370291
display id: annotation2370291
version: 1
top level: BBa_K1031019
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1031019/annotation2370291/range2370291/1

https://synbiohub.org/public/igem/BBa_K1031019/annotation2370291/range2370291/1
type: Range
persistent identity: range2370291
display id: range2370291
version: 1
direction: direction/reverse
top level: BBa_K1031019
start: 211
end: 1113
orientation: inline

phiR73delta
type: SequenceAnnotation
persistent identity: annotation2370294
display id: annotation2370294
version: 1
top level: BBa_K1031019
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1031019/annotation2370294/range2370294/1

https://synbiohub.org/public/igem/BBa_K1031019/annotation2370294/range2370294/1
type: Range
persistent identity: range2370294
display id: range2370294
version: 1
direction: direction/forward
top level: BBa_K1031019
start: 1370
end: 1615
orientation: inline

Pr
type: SequenceAnnotation
persistent identity: annotation2370292
display id: annotation2370292
version: 1
top level: BBa_K1031019
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1031019/annotation2370292/range2370292/1

https://synbiohub.org/public/igem/BBa_K1031019/annotation2370292/range2370292/1
type: Range
persistent identity: range2370292
display id: range2370292
version: 1
direction: direction/reverse
top level: BBa_K1031019
start: 1114
end: 1203
orientation: inline

Psal
type: SequenceAnnotation
persistent identity: annotation2370293
display id: annotation2370293
version: 1
top level: BBa_K1031019
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1031019/annotation2370293/range2370293/1

https://synbiohub.org/public/igem/BBa_K1031019/annotation2370293/range2370293/1
type: Range
persistent identity: range2370293
display id: range2370293
version: 1
direction: direction/forward
top level: BBa_K1031019
start: 1192
end: 1260
orientation: inline

BBa_K165095
type: ComponentDefinition
persistent identity: BBa K165095
display id: BBa_K165095
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Gli1 bs + LexA bs + mCYC + Zif268-HIV repressor (mCherryx2 tagged) on pRS303
creator: John Szymanski
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:10:57Z
discontinued: false
dominant: false
experience: experience/None
group u list: _267_
m user id: 0
owner id: 2512
owning group id: 58
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: -
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_K165002
component: BBa_K165004
component: BBa_K165003
component: BBa_K165012
component: BBa_K165031
component: BBa_K165006
component: BBa_K165018
sequence annotation: BBa_K165003
sequence annotation: BBa_K165031
sequence annotation: BBa_K165018
sequence annotation: BBa_K165002
sequence annotation: BBa_K165004
sequence annotation: BBa_K165006
sequence annotation: BBa_K165012
sequence: https://synbiohub.org/public/igem/BBa_K165095_sequence/1

BBa_K165002
type: Component
persistent identity: component1997344
display id: component1997344
version: 1
top level: BBa_K165095
access: public
definition: https://synbiohub.org/public/igem/BBa_K165002/1

BBa_K165004
type: Component
persistent identity: component1997347
display id: component1997347
version: 1
top level: BBa_K165095
access: public
definition: https://synbiohub.org/public/igem/BBa_K165004/1

BBa_K165003
type: Component
persistent identity: component1997345
display id: component1997345
version: 1
top level: BBa_K165095
access: public
definition: https://synbiohub.org/public/igem/BBa_K165003/1

BBa_K165012
type: Component
persistent identity: component1997342
display id: component1997342
version: 1
top level: BBa_K165095
access: public
definition: https://synbiohub.org/public/igem/BBa_K165012/1

BBa_K165004
type: Component
persistent identity: component1997348
display id: component1997348
version: 1
top level: BBa_K165095
access: public
definition: https://synbiohub.org/public/igem/BBa_K165004/1

BBa_K165031
type: Component
persistent identity: component1997343
display id: component1997343
version: 1
top level: BBa_K165095
access: public
definition: https://synbiohub.org/public/igem/BBa_K165031/1

BBa_K165006
type: Component
persistent identity: component1997346
display id: component1997346
version: 1
top level: BBa_K165095
access: public
definition: https://synbiohub.org/public/igem/BBa_K165006/1

BBa_K165018
type: Component
persistent identity: component1997349
display id: component1997349
version: 1
top level: BBa_K165095
access: public
definition: https://synbiohub.org/public/igem/BBa_K165018/1

BBa_K165003
type: SequenceAnnotation
persistent identity: annotation1997345
display id: annotation1997345
version: 1
top level: BBa_K165095
location: https://synbiohub.org/public/igem/BBa_K165095/annotation1997345/range1997345/1
component: BBa_K165003

https://synbiohub.org/public/igem/BBa_K165095/annotation1997345/range1997345/1
type: Range
persistent identity: range1997345
display id: range1997345
version: 1
direction: direction/reverse
top level: BBa_K165095
start: 573
end: 3383
orientation: inline

BBa_K165031
type: SequenceAnnotation
persistent identity: annotation1997343
display id: annotation1997343
version: 1
top level: BBa_K165095
location: https://synbiohub.org/public/igem/BBa_K165095/annotation1997343/range1997343/1
component: BBa_K165031

https://synbiohub.org/public/igem/BBa_K165095/annotation1997343/range1997343/1
type: Range
persistent identity: range1997343
display id: range1997343
version: 1
direction: direction/reverse
top level: BBa_K165095
start: 136
end: 538
orientation: inline

BBa_K165018
type: SequenceAnnotation
persistent identity: annotation1997349
display id: annotation1997349
version: 1
top level: BBa_K165095
location: https://synbiohub.org/public/igem/BBa_K165095/annotation1997349/range1997349/1
component: BBa_K165018

https://synbiohub.org/public/igem/BBa_K165095/annotation1997349/range1997349/1
type: Range
persistent identity: range1997349
display id: range1997349
version: 1
direction: direction/reverse
top level: BBa_K165095
start: 5138
end: 5390
orientation: inline

BBa_K165002
type: SequenceAnnotation
persistent identity: annotation1997344
display id: annotation1997344
version: 1
top level: BBa_K165095
location: https://synbiohub.org/public/igem/BBa_K165095/annotation1997344/range1997344/1
component: BBa_K165002

https://synbiohub.org/public/igem/BBa_K165095/annotation1997344/range1997344/1
type: Range
persistent identity: range1997344
display id: range1997344
version: 1
direction: direction/reverse
top level: BBa_K165095
start: 547
end: 564
orientation: inline

BBa_K165004
type: SequenceAnnotation
persistent identity: annotation1997347
display id: annotation1997347
version: 1
top level: BBa_K165095
location: https://synbiohub.org/public/igem/BBa_K165095/annotation1997347/range1997347/1
component: BBa_K165004

https://synbiohub.org/public/igem/BBa_K165095/annotation1997347/range1997347/1
type: Range
persistent identity: range1997347
display id: range1997347
version: 1
direction: direction/reverse
top level: BBa_K165095
start: 3652
end: 4386
orientation: inline

BBa_K165006
type: SequenceAnnotation
persistent identity: annotation1997346
display id: annotation1997346
version: 1
top level: BBa_K165095
location: https://synbiohub.org/public/igem/BBa_K165095/annotation1997346/range1997346/1
component: BBa_K165006

https://synbiohub.org/public/igem/BBa_K165095/annotation1997346/range1997346/1
type: Range
persistent identity: range1997346
display id: range1997346
version: 1
direction: direction/reverse
top level: BBa_K165095
start: 3392
end: 3643
orientation: inline

BBa_K165004
type: SequenceAnnotation
persistent identity: annotation1997348
display id: annotation1997348
version: 1
top level: BBa_K165095
location: https://synbiohub.org/public/igem/BBa_K165095/annotation1997348/range1997348/1
component: BBa_K165004

https://synbiohub.org/public/igem/BBa_K165095/annotation1997348/range1997348/1
type: Range
persistent identity: range1997348
display id: range1997348
version: 1
direction: direction/reverse
top level: BBa_K165095
start: 4395
end: 5129
orientation: inline

BBa_K165012
type: SequenceAnnotation
persistent identity: annotation1997342
display id: annotation1997342
version: 1
top level: BBa_K165095
location: https://synbiohub.org/public/igem/BBa_K165095/annotation1997342/range1997342/1
component: BBa_K165012

https://synbiohub.org/public/igem/BBa_K165095/annotation1997342/range1997342/1
type: Range
persistent identity: range1997342
display id: range1997342
version: 1
direction: direction/reverse
top level: BBa_K165095
start: 1
end: 127
orientation: inline

cinr
type: ComponentDefinition
persistent identity: BBa C0077
display id: BBa_C0077
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: cinR activator from Rhizobium leguminosarum (+LVA)
creator: crackdots
created: 2004-01-27T12:00:00Z
modified: 2015-08-31T04:07:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: In complex with O3-C14:1-HSL (made by BBa_C0076), CinR binds to the Cin promoter (BBa_R0077) and activates transcription.
mutable notes: The regulatory locus cinRI in Rhizobium leguminosarum conrols a network of quorum-sensing loci Lithgow, JK; Wilkinson, A; Hardman, A; Rodelas, B; Wisniewski-Dye, F; Williams, P; Downie, AJ MOL. MICROBIOLOGY 37(1): 81-97, 2000 Change log: original STOP: tag -> taATAA
mutable provenance: Rhizobium leguminosarum
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Help:Barcodes
sequence annotation: LVA
sequence annotation: cinR
sequence: https://synbiohub.org/public/igem/BBa_C0077_sequence/1

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2214000
display id: annotation2214000
version: 1
top level: cinr
role: SO:0000807
role: feature/barcode
location: https://synbiohub.org/public/igem/BBa_C0077/annotation2214000/range2214000/1

https://synbiohub.org/public/igem/BBa_C0077/annotation2214000/range2214000/1
type: Range
persistent identity: range2214000
display id: range2214000
version: 1
direction: direction/forward
top level: cinr
start: 763
end: 787
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation306600
display id: annotation306600
version: 1
top level: cinr
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_C0077/annotation306600/range306600/1

https://synbiohub.org/public/igem/BBa_C0077/annotation306600/range306600/1
type: Range
persistent identity: range306600
display id: range306600
version: 1
direction: direction/forward
top level: cinr
start: 724
end: 756
orientation: inline

cinR
type: SequenceAnnotation
persistent identity: annotation301112
display id: annotation301112
version: 1
top level: cinr
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_C0077/annotation301112/range301112/1

https://synbiohub.org/public/igem/BBa_C0077/annotation301112/range301112/1
type: Range
persistent identity: range301112
display id: range301112
version: 1
direction: direction/forward
top level: cinr
start: 1
end: 723
orientation: inline

BBa_K266002
type: ComponentDefinition
persistent identity: BBa K266002
display id: BBa_K266002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LasR + Term
creator: UNAM FCIENCIAS-CINVESTAV IRA
created: 2009-10-12T11:00:00Z
modified: 2015-05-08T01:11:43Z
discontinued: false
dominant: false
experience: experience/None
group u list: _363_
m user id: 0
owner id: 1955
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: LasR coding region and a double terminator
mutable notes: None
mutable provenance: The biobricks BBa_C0079 and BBa_B0015 where used for this biobrick.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_C0079
component: BBa_B0015
sequence annotation: BBa_C0079
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_K266002_sequence/1

BBa_C0079
type: Component
persistent identity: component2246664
display id: component2246664
version: 1
top level: BBa_K266002
access: public
definition: lasr

BBa_B0015
type: Component
persistent identity: component2246671
display id: component2246671
version: 1
top level: BBa_K266002
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_C0079
type: SequenceAnnotation
persistent identity: annotation2246664
display id: annotation2246664
version: 1
top level: BBa_K266002
location: https://synbiohub.org/public/igem/BBa_K266002/annotation2246664/range2246664/1
component: BBa_C0079

https://synbiohub.org/public/igem/BBa_K266002/annotation2246664/range2246664/1
type: Range
persistent identity: range2246664
display id: range2246664
version: 1
direction: direction/reverse
top level: BBa_K266002
start: 1
end: 781
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2246671
display id: annotation2246671
version: 1
top level: BBa_K266002
location: https://synbiohub.org/public/igem/BBa_K266002/annotation2246671/range2246671/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K266002/annotation2246671/range2246671/1
type: Range
persistent identity: range2246671
display id: range2246671
version: 1
direction: direction/reverse
top level: BBa_K266002
start: 790
end: 918
orientation: inline

lacI
type: ComponentDefinition
persistent identity: BBa C0012
display id: BBa_C0012
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: lacI repressor from E. coli (+LVA)
creator: Grace Kenney, Daniel Shen, Neelaksh Varshney, Samantha Sutton
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:23Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Coding region for the LacI protein with an LVA degradation tail and without an RBS. LacI binds to the pLac regulator <bb_part>BBa_R0010</bb_part> and PLlac01 hybrid regulator <bb_part>BBa_R0011</bb_part> and inhibits transcription. IPTG (Isopropylthiogalactoside) binds to LacI and inhibits its operation, therefore promoting transcription. A rapid degredation tail (LVA) has been added to improve the High to Low performance of this part.
mutable notes: References (unparsed) here: Elowitz, M.B., Leibler, S. A synthetic oscillatory network of transcriptional regulators. Nature 403, 335-338 (2000). [ELOW00] References (unparsed) here: Elowitz, M.B., Leibler, S. A synthetic oscillatory network of transcriptional regulators. Nature 403, 335-338 (2000). [ELOW00] Sequence taken from the repressilator of Elowitz and Leibler (2000). The obtained sequence was compared to the wild-type sequence for LacI obtained through a database search. The sequence had been modified from the wild-type in that wild-type GTG start was changed to an ATG start (note, actual ORF in E.coli has several GTG starts it would seem). The LVA tag has been added for quicker degradation. Incompatible with systems containing LacI, lactose, or IPTG.
mutable provenance: represillator of Elowitz and Leibler (2000)
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Help:Barcodes
sequence annotation: lacI-LVA
sequence annotation: BBa_C0012
sequence annotation: LVA
sequence: https://synbiohub.org/public/igem/BBa_C0012_sequence/1

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213988
display id: annotation2213988
version: 1
top level: lacI
role: SO:0000807
role: feature/barcode
location: https://synbiohub.org/public/igem/BBa_C0012/annotation2213988/range2213988/1

https://synbiohub.org/public/igem/BBa_C0012/annotation2213988/range2213988/1
type: Range
persistent identity: range2213988
display id: range2213988
version: 1
direction: direction/forward
top level: lacI
start: 1129
end: 1153
orientation: inline

lacI-LVA
type: SequenceAnnotation
persistent identity: annotation1723
display id: annotation1723
version: 1
top level: lacI
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_C0012/annotation1723/range1723/1

https://synbiohub.org/public/igem/BBa_C0012/annotation1723/range1723/1
type: Range
persistent identity: range1723
display id: range1723
version: 1
direction: direction/forward
top level: lacI
start: 1
end: 1128
orientation: inline

BBa_C0012
type: SequenceAnnotation
persistent identity: annotation7031
display id: annotation7031
version: 1
top level: lacI
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_C0012/annotation7031/range7031/1

https://synbiohub.org/public/igem/BBa_C0012/annotation7031/range7031/1
type: Range
persistent identity: range7031
display id: range7031
version: 1
direction: direction/forward
top level: lacI
start: 1
end: 1128
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation1722
display id: annotation1722
version: 1
top level: lacI
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_C0012/annotation1722/range1722/1

https://synbiohub.org/public/igem/BBa_C0012/annotation1722/range1722/1
type: Range
persistent identity: range1722
display id: range1722
version: 1
direction: direction/forward
top level: lacI
start: 1090
end: 1128
orientation: inline

BBa_I14044
type: ComponentDefinition
persistent identity: BBa I14044
display id: BBa_I14044
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: weak RBS . LacI
creator: Aditi Shrivastava, Jiwon Lee, Sarah Welch
created: 2004-08-03T11:00:00Z
modified: 2015-08-31T04:07:38Z
discontinued: false
dominant: false
experience: experience/None
group u list: _4_
m user id: 0
owner id: 166
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: BBa_B0031 BBa_C0012
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_C0012
component: BBa_B0031
sequence annotation: BBa_C0012
sequence annotation: BBa_B0031
sequence: https://synbiohub.org/public/igem/BBa_I14044_sequence/1

BBa_C0012
type: Component
persistent identity: component1041746
display id: component1041746
version: 1
top level: BBa_I14044
access: public
definition: lacI

BBa_B0031
type: Component
persistent identity: component1041736
display id: component1041736
version: 1
top level: BBa_I14044
access: public
definition: https://synbiohub.org/public/igem/BBa_B0031/1

BBa_C0012
type: SequenceAnnotation
persistent identity: annotation1041746
display id: annotation1041746
version: 1
top level: BBa_I14044
location: https://synbiohub.org/public/igem/BBa_I14044/annotation1041746/range1041746/1
component: BBa_C0012

https://synbiohub.org/public/igem/BBa_I14044/annotation1041746/range1041746/1
type: Range
persistent identity: range1041746
display id: range1041746
version: 1
direction: direction/forward
top level: BBa_I14044
start: 21
end: 1148
orientation: inline

BBa_B0031
type: SequenceAnnotation
persistent identity: annotation1041736
display id: annotation1041736
version: 1
top level: BBa_I14044
location: https://synbiohub.org/public/igem/BBa_I14044/annotation1041736/range1041736/1
component: BBa_B0031

https://synbiohub.org/public/igem/BBa_I14044/annotation1041736/range1041736/1
type: Range
persistent identity: range1041736
display id: range1041736
version: 1
direction: direction/forward
top level: BBa_I14044
start: 1
end: 14
orientation: inline

BBa_K1343000
type: ComponentDefinition
persistent identity: BBa K1343000
display id: BBa_K1343000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: P_tet->NheI->RBS->luxI->double terminator
creator: Shira Attias
created: 2014-09-21T11:00:00Z
modified: 2015-05-08T01:09:59Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1718_
m user id: 0
owner id: 20627
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This part generates AHL (Source organism: V. fischeri). The promoter is P_tet which induced by Tetracycline or by its analog anhydrotetracycline (aTc). Before the promoter there is the prefix site and after the promoter there is a restriction site of NheI, so the promoter can be replaced by a restriction enzyme reaction. In addition, after the NheI restriction site, there is an RBS and luxI which is coding to AHL. At the end of the part, there is double terminator and the Suffix site.
mutable notes: The part has been sequenced and has been checked with a detector strain which detects AHL and produce color. We know for sure that it produces AHL.
mutable provenance: P_tet: BBa_I759002- we used only the promoter, without the rest of the part (spacer (including the RBS)-cro complex) and added few bp as a spacer. RBS: BBa_B0030. luxI: BBa_C0061 but we removed the LVA tag and added stop codon: "tag". Double terminator BBa_B0015
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: P_tet
sequence annotation: spacer
sequence annotation: NheI
sequence annotation: Double terminator
sequence annotation: luxI
sequence: https://synbiohub.org/public/igem/BBa_K1343000_sequence/1

P_tet
type: SequenceAnnotation
persistent identity: annotation2384865
display id: annotation2384865
version: 1
top level: BBa_K1343000
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1343000/annotation2384865/range2384865/1

https://synbiohub.org/public/igem/BBa_K1343000/annotation2384865/range2384865/1
type: Range
persistent identity: range2384865
display id: range2384865
version: 1
direction: direction/forward
top level: BBa_K1343000
start: 1
end: 63
orientation: inline

spacer
type: SequenceAnnotation
persistent identity: annotation2384871
display id: annotation2384871
version: 1
top level: BBa_K1343000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1343000/annotation2384871/range2384871/1

https://synbiohub.org/public/igem/BBa_K1343000/annotation2384871/range2384871/1
type: Range
persistent identity: range2384871
display id: range2384871
version: 1
direction: direction/forward
top level: BBa_K1343000
start: 70
end: 90
orientation: inline

NheI
type: SequenceAnnotation
persistent identity: annotation2384866
display id: annotation2384866
version: 1
top level: BBa_K1343000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1343000/annotation2384866/range2384866/1

https://synbiohub.org/public/igem/BBa_K1343000/annotation2384866/range2384866/1
type: Range
persistent identity: range2384866
display id: range2384866
version: 1
direction: direction/forward
top level: BBa_K1343000
start: 64
end: 69
orientation: inline

Double terminator
type: SequenceAnnotation
persistent identity: annotation2384874
display id: annotation2384874
version: 1
top level: BBa_K1343000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1343000/annotation2384874/range2384874/1

https://synbiohub.org/public/igem/BBa_K1343000/annotation2384874/range2384874/1
type: Range
persistent identity: range2384874
display id: range2384874
version: 1
direction: direction/forward
top level: BBa_K1343000
start: 673
end: 801
orientation: inline

luxI
type: SequenceAnnotation
persistent identity: annotation2384872
display id: annotation2384872
version: 1
top level: BBa_K1343000
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1343000/annotation2384872/range2384872/1

https://synbiohub.org/public/igem/BBa_K1343000/annotation2384872/range2384872/1
type: Range
persistent identity: range2384872
display id: range2384872
version: 1
direction: direction/forward
top level: BBa_K1343000
start: 91
end: 672
orientation: inline

PAtpI
type: ComponentDefinition
persistent identity: BBa K1930005
display id: BBa_K1930005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PAtpI
creator: Eike Mahlandt
created: 2016-10-15T11:00:00Z
modified: 2016-10-16T04:58:48Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2397_
m user id: 29748
owner id: 29748
owning group id: 9
status: status/Planning
bookmark: false
mutable description: The promoter PAtpI has its origin in Bacillus subtilis. It is responsible for the expression of atpA gene (ATP synthesis) during the first 30 min of germination [https://www.ncbi.nlm.nih.gov/pubmed/25661487 Sinai''et al.'']. This gene is part of an operon, therefore the promoter region in front of the first protein coding gene (atpI) in this operon was chosen. The region was checked for the binding of sigma factors and transcription factors with DBTBS. No binding factors were found with the highest significance level. In our project we wanted to find a constitutive ON promoter for our ciprofloxacin resistance casette. In the following part we put the promoter PAtpI in the pSB1C3 backbone to make it available to other iGEM teams.
mutable notes: The promoter PAtpI has its origin in Bacillus subtilis. It is responsible for the expression of atpA gene (ATP synthesis) during the first 30 min of germination [https://www.ncbi.nlm.nih.gov/pubmed/25661487 Sinai''et al.'']. This gene is part of an operon, therefore the promoter region in front of the first protein coding gene (atpI) in this operon was chosen. The region was checked for the binding of sigma factors and transcription factors with DBTBS. No binding factors were found with the highest significance level. In our project we wanted to find a constitutive ON promoter for our ciprofloxacin resistance casette. In the following part we put the promoter PAtpI in the pSB1C3 backbone to make it available to other iGEM teams.
mutable provenance: Ordered as gBlock. IDT (Integrated DNA technologies).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: P(AtpI)
sequence: https://synbiohub.org/public/igem/BBa_K1930005_sequence/1

P(AtpI)
type: SequenceAnnotation
persistent identity: annotation2513454
display id: annotation2513454
version: 1
top level: PAtpI
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1930005/annotation2513454/range2513454/1

https://synbiohub.org/public/igem/BBa_K1930005/annotation2513454/range2513454/1
type: Range
persistent identity: range2513454
display id: range2513454
version: 1
direction: direction/forward
top level: PAtpI
start: 1
end: 349
orientation: inline

BBa_K1031441
type: ComponentDefinition
persistent identity: BBa K1031441
display id: BBa_K1031441
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: J23106-HcaR
creator: He Shuaixin
created: 2013-09-08T11:00:00Z
modified: 2015-05-08T01:08:47Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1338_
m user id: 0
owner id: 15843
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: 106-HcaR-TT
mutable notes: molecular cloning
mutable provenance: PCR genome E.coli
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: BBa_B0034
sequence annotation: HcaR
sequence annotation: BBa_J23106
sequence: https://synbiohub.org/public/igem/BBa_K1031441_sequence/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2357454
display id: annotation2357454
version: 1
top level: BBa_K1031441
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1031441/annotation2357454/range2357454/1

https://synbiohub.org/public/igem/BBa_K1031441/annotation2357454/range2357454/1
type: Range
persistent identity: range2357454
display id: range2357454
version: 1
direction: direction/forward
top level: BBa_K1031441
start: 44
end: 55
orientation: inline

HcaR
type: SequenceAnnotation
persistent identity: annotation2357455
display id: annotation2357455
version: 1
top level: BBa_K1031441
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1031441/annotation2357455/range2357455/1

https://synbiohub.org/public/igem/BBa_K1031441/annotation2357455/range2357455/1
type: Range
persistent identity: range2357455
display id: range2357455
version: 1
direction: direction/forward
top level: BBa_K1031441
start: 62
end: 952
orientation: inline

BBa_J23106
type: SequenceAnnotation
persistent identity: annotation2357453
display id: annotation2357453
version: 1
top level: BBa_K1031441
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1031441/annotation2357453/range2357453/1

https://synbiohub.org/public/igem/BBa_K1031441/annotation2357453/range2357453/1
type: Range
persistent identity: range2357453
display id: range2357453
version: 1
direction: direction/forward
top level: BBa_K1031441
start: 1
end: 35
orientation: inline

BBa_K165091
type: ComponentDefinition
persistent identity: BBa K165091
display id: BBa_K165091
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Zif268-HIV bs + LexA bs + mCYC + Zif268-HIV repressor (mCherryx2 tagged) on pRS306
creator: John Szymanski
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:10:57Z
discontinued: false
dominant: true
group u list: _267_
m user id: 0
owner id: 2512
owning group id: 58
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: -
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_K165002
component: BBa_K165006
component: BBa_K165004
component: BBa_K165031
component: BBa_K165011
component: BBa_K165018
component: BBa_K165003
sequence annotation: BBa_K165004
sequence annotation: BBa_K165018
sequence annotation: BBa_K165003
sequence annotation: BBa_K165031
sequence annotation: BBa_K165011
sequence annotation: BBa_K165002
sequence annotation: BBa_K165006
sequence: https://synbiohub.org/public/igem/BBa_K165091_sequence/1

BBa_K165002
type: Component
persistent identity: component1997280
display id: component1997280
version: 1
top level: BBa_K165091
access: public
definition: https://synbiohub.org/public/igem/BBa_K165002/1

BBa_K165006
type: Component
persistent identity: component1997282
display id: component1997282
version: 1
top level: BBa_K165091
access: public
definition: https://synbiohub.org/public/igem/BBa_K165006/1

BBa_K165004
type: Component
persistent identity: component1997283
display id: component1997283
version: 1
top level: BBa_K165091
access: public
definition: https://synbiohub.org/public/igem/BBa_K165004/1

BBa_K165031
type: Component
persistent identity: component1997279
display id: component1997279
version: 1
top level: BBa_K165091
access: public
definition: https://synbiohub.org/public/igem/BBa_K165031/1

BBa_K165011
type: Component
persistent identity: component1997278
display id: component1997278
version: 1
top level: BBa_K165091
access: public
definition: https://synbiohub.org/public/igem/BBa_K165011/1

BBa_K165018
type: Component
persistent identity: component1997285
display id: component1997285
version: 1
top level: BBa_K165091
access: public
definition: https://synbiohub.org/public/igem/BBa_K165018/1

BBa_K165004
type: Component
persistent identity: component1997284
display id: component1997284
version: 1
top level: BBa_K165091
access: public
definition: https://synbiohub.org/public/igem/BBa_K165004/1

BBa_K165003
type: Component
persistent identity: component1997281
display id: component1997281
version: 1
top level: BBa_K165091
access: public
definition: https://synbiohub.org/public/igem/BBa_K165003/1

BBa_K165004
type: SequenceAnnotation
persistent identity: annotation1997283
display id: annotation1997283
version: 1
top level: BBa_K165091
location: https://synbiohub.org/public/igem/BBa_K165091/annotation1997283/range1997283/1
component: BBa_K165004

https://synbiohub.org/public/igem/BBa_K165091/annotation1997283/range1997283/1
type: Range
persistent identity: range1997283
display id: range1997283
version: 1
direction: direction/reverse
top level: BBa_K165091
start: 3571
end: 4305
orientation: inline

BBa_K165004
type: SequenceAnnotation
persistent identity: annotation1997284
display id: annotation1997284
version: 1
top level: BBa_K165091
location: https://synbiohub.org/public/igem/BBa_K165091/annotation1997284/range1997284/1
component: BBa_K165004

https://synbiohub.org/public/igem/BBa_K165091/annotation1997284/range1997284/1
type: Range
persistent identity: range1997284
display id: range1997284
version: 1
direction: direction/reverse
top level: BBa_K165091
start: 4314
end: 5048
orientation: inline

BBa_K165018
type: SequenceAnnotation
persistent identity: annotation1997285
display id: annotation1997285
version: 1
top level: BBa_K165091
location: https://synbiohub.org/public/igem/BBa_K165091/annotation1997285/range1997285/1
component: BBa_K165018

https://synbiohub.org/public/igem/BBa_K165091/annotation1997285/range1997285/1
type: Range
persistent identity: range1997285
display id: range1997285
version: 1
direction: direction/reverse
top level: BBa_K165091
start: 5057
end: 5309
orientation: inline

BBa_K165003
type: SequenceAnnotation
persistent identity: annotation1997281
display id: annotation1997281
version: 1
top level: BBa_K165091
location: https://synbiohub.org/public/igem/BBa_K165091/annotation1997281/range1997281/1
component: BBa_K165003

https://synbiohub.org/public/igem/BBa_K165091/annotation1997281/range1997281/1
type: Range
persistent identity: range1997281
display id: range1997281
version: 1
direction: direction/reverse
top level: BBa_K165091
start: 492
end: 3302
orientation: inline

BBa_K165031
type: SequenceAnnotation
persistent identity: annotation1997279
display id: annotation1997279
version: 1
top level: BBa_K165091
location: https://synbiohub.org/public/igem/BBa_K165091/annotation1997279/range1997279/1
component: BBa_K165031

https://synbiohub.org/public/igem/BBa_K165091/annotation1997279/range1997279/1
type: Range
persistent identity: range1997279
display id: range1997279
version: 1
direction: direction/reverse
top level: BBa_K165091
start: 55
end: 457
orientation: inline

BBa_K165011
type: SequenceAnnotation
persistent identity: annotation1997278
display id: annotation1997278
version: 1
top level: BBa_K165091
location: https://synbiohub.org/public/igem/BBa_K165091/annotation1997278/range1997278/1
component: BBa_K165011

https://synbiohub.org/public/igem/BBa_K165091/annotation1997278/range1997278/1
type: Range
persistent identity: range1997278
display id: range1997278
version: 1
direction: direction/reverse
top level: BBa_K165091
start: 1
end: 46
orientation: inline

BBa_K165002
type: SequenceAnnotation
persistent identity: annotation1997280
display id: annotation1997280
version: 1
top level: BBa_K165091
location: https://synbiohub.org/public/igem/BBa_K165091/annotation1997280/range1997280/1
component: BBa_K165002

https://synbiohub.org/public/igem/BBa_K165091/annotation1997280/range1997280/1
type: Range
persistent identity: range1997280
display id: range1997280
version: 1
direction: direction/reverse
top level: BBa_K165091
start: 466
end: 483
orientation: inline

BBa_K165006
type: SequenceAnnotation
persistent identity: annotation1997282
display id: annotation1997282
version: 1
top level: BBa_K165091
location: https://synbiohub.org/public/igem/BBa_K165091/annotation1997282/range1997282/1
component: BBa_K165006

https://synbiohub.org/public/igem/BBa_K165091/annotation1997282/range1997282/1
type: Range
persistent identity: range1997282
display id: range1997282
version: 1
direction: direction/reverse
top level: BBa_K165091
start: 3311
end: 3562
orientation: inline

BBa_K1031742
type: ComponentDefinition
persistent identity: BBa K1031742
display id: BBa_K1031742
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: J23109-PaaX
creator: He Shuaixin
created: 2013-09-08T11:00:00Z
modified: 2015-05-08T01:08:47Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1338_
m user id: 0
owner id: 15843
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: 109-PaaX-TT
mutable notes: molecular cloning
mutable provenance: PCR genome E.coli
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: BBa_B0034
sequence annotation: BBa_J23109
sequence annotation: PaaX
sequence: https://synbiohub.org/public/igem/BBa_K1031742_sequence/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2357490
display id: annotation2357490
version: 1
top level: BBa_K1031742
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1031742/annotation2357490/range2357490/1

https://synbiohub.org/public/igem/BBa_K1031742/annotation2357490/range2357490/1
type: Range
persistent identity: range2357490
display id: range2357490
version: 1
direction: direction/forward
top level: BBa_K1031742
start: 44
end: 55
orientation: inline

BBa_J23109
type: SequenceAnnotation
persistent identity: annotation2357488
display id: annotation2357488
version: 1
top level: BBa_K1031742
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1031742/annotation2357488/range2357488/1

https://synbiohub.org/public/igem/BBa_K1031742/annotation2357488/range2357488/1
type: Range
persistent identity: range2357488
display id: range2357488
version: 1
direction: direction/forward
top level: BBa_K1031742
start: 1
end: 35
orientation: inline

PaaX
type: SequenceAnnotation
persistent identity: annotation2357489
display id: annotation2357489
version: 1
top level: BBa_K1031742
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1031742/annotation2357489/range2357489/1

https://synbiohub.org/public/igem/BBa_K1031742/annotation2357489/range2357489/1
type: Range
persistent identity: range2357489
display id: range2357489
version: 1
direction: direction/forward
top level: BBa_K1031742
start: 62
end: 1012
orientation: inline

BBa_K165080
type: ComponentDefinition
persistent identity: BBa K165080
display id: BBa_K165080
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pGAL1 + Untagged LexA activator on pRS305
creator: John Szymanski
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:10:57Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _267_
m user id: 0
owner id: 2512
owning group id: 58
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: -
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_K165046
component: BBa_K165020
sequence annotation: BBa_K165020
sequence annotation: BBa_K165046
sequence: https://synbiohub.org/public/igem/BBa_K165080_sequence/1

BBa_K165046
type: Component
persistent identity: component1997135
display id: component1997135
version: 1
top level: BBa_K165080
access: public
definition: https://synbiohub.org/public/igem/BBa_K165046/1

BBa_K165020
type: Component
persistent identity: component1997136
display id: component1997136
version: 1
top level: BBa_K165080
access: public
definition: lexA

BBa_K165020
type: SequenceAnnotation
persistent identity: annotation1997136
display id: annotation1997136
version: 1
top level: BBa_K165080
location: https://synbiohub.org/public/igem/BBa_K165080/annotation1997136/range1997136/1
component: BBa_K165020

https://synbiohub.org/public/igem/BBa_K165080/annotation1997136/range1997136/1
type: Range
persistent identity: range1997136
display id: range1997136
version: 1
direction: direction/reverse
top level: BBa_K165080
start: 558
end: 1229
orientation: inline

BBa_K165046
type: SequenceAnnotation
persistent identity: annotation1997135
display id: annotation1997135
version: 1
top level: BBa_K165080
location: https://synbiohub.org/public/igem/BBa_K165080/annotation1997135/range1997135/1
component: BBa_K165046

https://synbiohub.org/public/igem/BBa_K165080/annotation1997135/range1997135/1
type: Range
persistent identity: range1997135
display id: range1997135
version: 1
direction: direction/reverse
top level: BBa_K165080
start: 1
end: 549
orientation: inline

BBa_K544002
type: ComponentDefinition
persistent identity: BBa K544002
display id: BBa_K544002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Tetracycline Repressor with Consitutive Promoter BBa_J23109
creator: Leung Man Hong
created: 2011-09-29T11:00:00Z
modified: 2015-05-08T01:12:39Z
discontinued: false
dominant: false
experience: experience/None
group u list: _711_
m user id: 0
owner id: 6780
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: A transcriptional regulator TetR (BBa_C0040) is driven by constitutive promoter family members (BBa-J23103, BBa-J23109, BBa-J23116, and BBa-J23106), which can be used to tune the expression level of constitutively expressed TetR.
mutable notes: The SsrA site at the end of BBa_C0040 is not present in our design.
mutable provenance: The source of this part is based on the registry distribution
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: cds
sequence annotation: BBa_B0034
sequence annotation: BBa_J23109
sequence: https://synbiohub.org/public/igem/BBa_K544002_sequence/1

cds
type: SequenceAnnotation
persistent identity: annotation2147988
display id: annotation2147988
version: 1
top level: BBa_K544002
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K544002/annotation2147988/range2147988/1

https://synbiohub.org/public/igem/BBa_K544002/annotation2147988/range2147988/1
type: Range
persistent identity: range2147988
display id: range2147988
version: 1
direction: direction/forward
top level: BBa_K544002
start: 62
end: 682
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2147987
display id: annotation2147987
version: 1
top level: BBa_K544002
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K544002/annotation2147987/range2147987/1

https://synbiohub.org/public/igem/BBa_K544002/annotation2147987/range2147987/1
type: Range
persistent identity: range2147987
display id: range2147987
version: 1
direction: direction/forward
top level: BBa_K544002
start: 44
end: 55
orientation: inline

BBa_J23109
type: SequenceAnnotation
persistent identity: annotation2147986
display id: annotation2147986
version: 1
top level: BBa_K544002
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K544002/annotation2147986/range2147986/1

https://synbiohub.org/public/igem/BBa_K544002/annotation2147986/range2147986/1
type: Range
persistent identity: range2147986
display id: range2147986
version: 1
direction: direction/forward
top level: BBa_K544002
start: 1
end: 35
orientation: inline

BBa_K1520012
type: ComponentDefinition
persistent identity: BBa K1520012
display id: BBa_K1520012
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Prlux-rbs-rfp-rbs-luxR-Ter
creator: yue wu
created: 2014-09-21T11:00:00Z
modified: 2015-05-08T01:10:49Z
discontinued: false
dominant: false
group u list: _1902_
m user id: 0
owner id: 21318
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Prlux-rbs-rfp-rbs-luxR-Ter. It produces rfp and luxR when there is enough rfp and luxR.
mutable notes: None
mutable provenance: Prlux, rfp, luxR, Ter
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1520029
component: BBa_K1520500
component: BBa_K1520501
component: BBa_R0062
sequence annotation: BBa_K1520501
sequence annotation: BBa_K1520500
sequence annotation: BBa_R0062
sequence annotation: BBa_K1520029
sequence: https://synbiohub.org/public/igem/BBa_K1520012_sequence/1

BBa_K1520029
type: Component
persistent identity: component2384977
display id: component2384977
version: 1
top level: BBa_K1520012
access: public
definition: https://synbiohub.org/public/igem/BBa_K1520029/1

BBa_K1520500
type: Component
persistent identity: component2384973
display id: component2384973
version: 1
top level: BBa_K1520012
access: public
definition: https://synbiohub.org/public/igem/BBa_K1520500/1

BBa_K1520501
type: Component
persistent identity: component2384976
display id: component2384976
version: 1
top level: BBa_K1520012
access: public
definition: BBa_K1520501

BBa_R0062
type: Component
persistent identity: component2384968
display id: component2384968
version: 1
top level: BBa_K1520012
access: public
definition: https://synbiohub.org/public/igem/BBa_R0062/1

BBa_K1520501
type: SequenceAnnotation
persistent identity: annotation2384976
display id: annotation2384976
version: 1
top level: BBa_K1520012
location: https://synbiohub.org/public/igem/BBa_K1520012/annotation2384976/range2384976/1
component: BBa_K1520501

https://synbiohub.org/public/igem/BBa_K1520012/annotation2384976/range2384976/1
type: Range
persistent identity: range2384976
display id: range2384976
version: 1
direction: direction/reverse
top level: BBa_K1520012
start: 804
end: 1602
orientation: inline

BBa_K1520500
type: SequenceAnnotation
persistent identity: annotation2384973
display id: annotation2384973
version: 1
top level: BBa_K1520012
location: https://synbiohub.org/public/igem/BBa_K1520012/annotation2384973/range2384973/1
component: BBa_K1520500

https://synbiohub.org/public/igem/BBa_K1520012/annotation2384973/range2384973/1
type: Range
persistent identity: range2384973
display id: range2384973
version: 1
direction: direction/reverse
top level: BBa_K1520012
start: 64
end: 795
orientation: inline

BBa_R0062
type: SequenceAnnotation
persistent identity: annotation2384968
display id: annotation2384968
version: 1
top level: BBa_K1520012
location: https://synbiohub.org/public/igem/BBa_K1520012/annotation2384968/range2384968/1
component: BBa_R0062

https://synbiohub.org/public/igem/BBa_K1520012/annotation2384968/range2384968/1
type: Range
persistent identity: range2384968
display id: range2384968
version: 1
direction: direction/forward
top level: BBa_K1520012
start: 1
end: 55
orientation: inline

BBa_K1520029
type: SequenceAnnotation
persistent identity: annotation2384977
display id: annotation2384977
version: 1
top level: BBa_K1520012
location: https://synbiohub.org/public/igem/BBa_K1520012/annotation2384977/range2384977/1
component: BBa_K1520029

https://synbiohub.org/public/igem/BBa_K1520012/annotation2384977/range2384977/1
type: Range
persistent identity: range2384977
display id: range2384977
version: 1
direction: direction/reverse
top level: BBa_K1520012
start: 1611
end: 1739
orientation: inline

BBa_K1072023
type: ComponentDefinition
persistent identity: BBa K1072023
display id: BBa_K1072023
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Fus1 Promotor
creator: junjie zhang
created: 2013-09-14T11:00:00Z
modified: 2015-05-08T01:09:01Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1381_
m user id: 0
owner id: 16002
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: BBa_K1072023 is a induced promotor, originating from S.cerevisiae.
mutable notes: -
mutable provenance: Originating from S.cerevisiae
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: Fus1
sequence: https://synbiohub.org/public/igem/BBa_K1072023_sequence/1

Fus1
type: SequenceAnnotation
persistent identity: annotation2342866
display id: annotation2342866
version: 1
top level: BBa_K1072023
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1072023/annotation2342866/range2342866/1

https://synbiohub.org/public/igem/BBa_K1072023/annotation2342866/range2342866/1
type: Range
persistent identity: range2342866
display id: range2342866
version: 1
direction: direction/forward
top level: BBa_K1072023
start: 1
end: 438
orientation: inline

BBa_K1861040
type: ComponentDefinition
persistent identity: BBa K1861040
display id: BBa_K1861040
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Fusion protein of SpyCatcher and RPD3 in an expression cassette
creator: Vladyslav Vyshnevskyi
created: 2015-09-17T11:00:00Z
modified: 2015-09-23T04:21:28Z
discontinued: false
dominant: false
group u list: _2291_
m user id: 25348
owner id: 25348
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Coming soon.
mutable notes: Coming soon.
mutable provenance: Coming soon.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1861101
component: BBa_K1861102
component: BBa_K1861104
component: BBa_K1159200
component: BBa_K392003
component: BBa_K1861401
sequence annotation: BBa_K1861401
sequence annotation: BBa_K1861102
sequence annotation: BBa_K1861101
sequence annotation: BBa_K1861104
sequence annotation: BBa_K392003
sequence annotation: BBa_K1159200
sequence: https://synbiohub.org/public/igem/BBa_K1861040_sequence/1

BBa_K1861101
type: Component
persistent identity: component2474400
display id: component2474400
version: 1
top level: BBa_K1861040
access: public
definition: https://synbiohub.org/public/igem/BBa_K1861101/1

BBa_K1861102
type: Component
persistent identity: component2474401
display id: component2474401
version: 1
top level: BBa_K1861040
access: public
definition: https://synbiohub.org/public/igem/BBa_K1861102/1

BBa_K1861104
type: Component
persistent identity: component2474408
display id: component2474408
version: 1
top level: BBa_K1861040
access: public
definition: https://synbiohub.org/public/igem/BBa_K1861104/1

BBa_K1159200
type: Component
persistent identity: component2474406
display id: component2474406
version: 1
top level: BBa_K1861040
access: public
definition: https://synbiohub.org/public/igem/BBa_K1159200/1

BBa_K392003
type: Component
persistent identity: component2474409
display id: component2474409
version: 1
top level: BBa_K1861040
access: public
definition: https://synbiohub.org/public/igem/BBa_K392003/1

BBa_K1861401
type: Component
persistent identity: component2474407
display id: component2474407
version: 1
top level: BBa_K1861040
access: public
definition: https://synbiohub.org/public/igem/BBa_K1861401/1

BBa_K1861401
type: SequenceAnnotation
persistent identity: annotation2474407
display id: annotation2474407
version: 1
top level: BBa_K1861040
location: https://synbiohub.org/public/igem/BBa_K1861040/annotation2474407/range2474407/1
component: BBa_K1861401

https://synbiohub.org/public/igem/BBa_K1861040/annotation2474407/range2474407/1
type: Range
persistent identity: range2474407
display id: range2474407
version: 1
direction: direction/reverse
top level: BBa_K1861040
start: 605
end: 1954
orientation: inline

BBa_K1861102
type: SequenceAnnotation
persistent identity: annotation2474401
display id: annotation2474401
version: 1
top level: BBa_K1861040
location: https://synbiohub.org/public/igem/BBa_K1861040/annotation2474401/range2474401/1
component: BBa_K1861102

https://synbiohub.org/public/igem/BBa_K1861040/annotation2474401/range2474401/1
type: Range
persistent identity: range2474401
display id: range2474401
version: 1
direction: direction/reverse
top level: BBa_K1861040
start: 239
end: 265
orientation: inline

BBa_K1861101
type: SequenceAnnotation
persistent identity: annotation2474400
display id: annotation2474400
version: 1
top level: BBa_K1861040
location: https://synbiohub.org/public/igem/BBa_K1861040/annotation2474400/range2474400/1
component: BBa_K1861101

https://synbiohub.org/public/igem/BBa_K1861040/annotation2474400/range2474400/1
type: Range
persistent identity: range2474400
display id: range2474400
version: 1
direction: direction/reverse
top level: BBa_K1861040
start: 1
end: 238
orientation: inline

BBa_K1861104
type: SequenceAnnotation
persistent identity: annotation2474408
display id: annotation2474408
version: 1
top level: BBa_K1861040
location: https://synbiohub.org/public/igem/BBa_K1861040/annotation2474408/range2474408/1
component: BBa_K1861104

https://synbiohub.org/public/igem/BBa_K1861040/annotation2474408/range2474408/1
type: Range
persistent identity: range2474408
display id: range2474408
version: 1
direction: direction/reverse
top level: BBa_K1861040
start: 1955
end: 1963
orientation: inline

BBa_K392003
type: SequenceAnnotation
persistent identity: annotation2474409
display id: annotation2474409
version: 1
top level: BBa_K1861040
location: https://synbiohub.org/public/igem/BBa_K1861040/annotation2474409/range2474409/1
component: BBa_K392003

https://synbiohub.org/public/igem/BBa_K1861040/annotation2474409/range2474409/1
type: Range
persistent identity: range2474409
display id: range2474409
version: 1
direction: direction/reverse
top level: BBa_K1861040
start: 1964
end: 2092
orientation: inline

BBa_K1159200
type: SequenceAnnotation
persistent identity: annotation2474406
display id: annotation2474406
version: 1
top level: BBa_K1861040
location: https://synbiohub.org/public/igem/BBa_K1861040/annotation2474406/range2474406/1
component: BBa_K1159200

https://synbiohub.org/public/igem/BBa_K1861040/annotation2474406/range2474406/1
type: Range
persistent identity: range2474406
display id: range2474406
version: 1
direction: direction/reverse
top level: BBa_K1861040
start: 266
end: 604
orientation: inline

BBa_K165081
type: ComponentDefinition
persistent identity: BBa K165081
display id: BBa_K165081
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pGAL1 + Untagged Zif268-HIV activator on pRS305
creator: John Szymanski
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:10:57Z
discontinued: false
dominant: false
group u list: _267_
m user id: 0
owner id: 2512
owning group id: 58
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: -
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_K165046
component: BBa_K165022
sequence annotation: BBa_K165022
sequence annotation: BBa_K165046
sequence: https://synbiohub.org/public/igem/BBa_K165081_sequence/1

BBa_K165046
type: Component
persistent identity: component1997137
display id: component1997137
version: 1
top level: BBa_K165081
access: public
definition: https://synbiohub.org/public/igem/BBa_K165046/1

BBa_K165022
type: Component
persistent identity: component1997138
display id: component1997138
version: 1
top level: BBa_K165081
access: public
definition: BBa_K165022

BBa_K165022
type: SequenceAnnotation
persistent identity: annotation1997138
display id: annotation1997138
version: 1
top level: BBa_K165081
location: https://synbiohub.org/public/igem/BBa_K165081/annotation1997138/range1997138/1
component: BBa_K165022

https://synbiohub.org/public/igem/BBa_K165081/annotation1997138/range1997138/1
type: Range
persistent identity: range1997138
display id: range1997138
version: 1
direction: direction/reverse
top level: BBa_K165081
start: 558
end: 1223
orientation: inline

BBa_K165046
type: SequenceAnnotation
persistent identity: annotation1997137
display id: annotation1997137
version: 1
top level: BBa_K165081
location: https://synbiohub.org/public/igem/BBa_K165081/annotation1997137/range1997137/1
component: BBa_K165046

https://synbiohub.org/public/igem/BBa_K165081/annotation1997137/range1997137/1
type: Range
persistent identity: range1997137
display id: range1997137
version: 1
direction: direction/reverse
top level: BBa_K165081
start: 1
end: 549
orientation: inline

BBa_K165053
type: ComponentDefinition
persistent identity: BBa K165053
display id: BBa_K165053
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Full LexA Repressor (tagged with CFPx2)
creator: John Szymanski
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:10:56Z
discontinued: false
dominant: false
experience: experience/None
group u list: _267_
m user id: 0
owner id: 2512
owning group id: 58
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: Kozak+Sin3p RD+LexA DBD+CFPx2+SV40NLS+tADH1
mutable notes: -
mutable provenance: Constructed in our lab.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K165009
component: BBa_K165002
component: BBa_K165003
component: BBa_K165018
component: BBa_K165014
sequence annotation: BBa_K165003
sequence annotation: BBa_K165009
sequence annotation: BBa_K165002
sequence annotation: BBa_K165018
sequence annotation: BBa_K165014
sequence: https://synbiohub.org/public/igem/BBa_K165053_sequence/1

BBa_K165009
type: Component
persistent identity: component1996731
display id: component1996731
version: 1
top level: BBa_K165053
access: public
definition: https://synbiohub.org/public/igem/BBa_K165009/1

BBa_K165002
type: Component
persistent identity: component1996730
display id: component1996730
version: 1
top level: BBa_K165053
access: public
definition: https://synbiohub.org/public/igem/BBa_K165002/1

BBa_K165003
type: Component
persistent identity: component1996732
display id: component1996732
version: 1
top level: BBa_K165053
access: public
definition: https://synbiohub.org/public/igem/BBa_K165003/1

BBa_K165018
type: Component
persistent identity: component1996734
display id: component1996734
version: 1
top level: BBa_K165053
access: public
definition: https://synbiohub.org/public/igem/BBa_K165018/1

BBa_K165014
type: Component
persistent identity: component1996733
display id: component1996733
version: 1
top level: BBa_K165053
access: public
definition: https://synbiohub.org/public/igem/BBa_K165014/1

BBa_K165003
type: SequenceAnnotation
persistent identity: annotation1996732
display id: annotation1996732
version: 1
top level: BBa_K165053
location: https://synbiohub.org/public/igem/BBa_K165053/annotation1996732/range1996732/1
component: BBa_K165003

https://synbiohub.org/public/igem/BBa_K165053/annotation1996732/range1996732/1
type: Range
persistent identity: range1996732
display id: range1996732
version: 1
direction: direction/reverse
top level: BBa_K165053
start: 293
end: 3103
orientation: inline

BBa_K165009
type: SequenceAnnotation
persistent identity: annotation1996731
display id: annotation1996731
version: 1
top level: BBa_K165053
location: https://synbiohub.org/public/igem/BBa_K165053/annotation1996731/range1996731/1
component: BBa_K165009

https://synbiohub.org/public/igem/BBa_K165053/annotation1996731/range1996731/1
type: Range
persistent identity: range1996731
display id: range1996731
version: 1
direction: direction/reverse
top level: BBa_K165053
start: 27
end: 284
orientation: inline

BBa_K165002
type: SequenceAnnotation
persistent identity: annotation1996730
display id: annotation1996730
version: 1
top level: BBa_K165053
location: https://synbiohub.org/public/igem/BBa_K165053/annotation1996730/range1996730/1
component: BBa_K165002

https://synbiohub.org/public/igem/BBa_K165053/annotation1996730/range1996730/1
type: Range
persistent identity: range1996730
display id: range1996730
version: 1
direction: direction/reverse
top level: BBa_K165053
start: 1
end: 18
orientation: inline

BBa_K165018
type: SequenceAnnotation
persistent identity: annotation1996734
display id: annotation1996734
version: 1
top level: BBa_K165053
location: https://synbiohub.org/public/igem/BBa_K165053/annotation1996734/range1996734/1
component: BBa_K165018

https://synbiohub.org/public/igem/BBa_K165053/annotation1996734/range1996734/1
type: Range
persistent identity: range1996734
display id: range1996734
version: 1
direction: direction/reverse
top level: BBa_K165053
start: 4614
end: 4866
orientation: inline

BBa_K165014
type: SequenceAnnotation
persistent identity: annotation1996733
display id: annotation1996733
version: 1
top level: BBa_K165053
location: https://synbiohub.org/public/igem/BBa_K165053/annotation1996733/range1996733/1
component: BBa_K165014

https://synbiohub.org/public/igem/BBa_K165053/annotation1996733/range1996733/1
type: Range
persistent identity: range1996733
display id: range1996733
version: 1
direction: direction/reverse
top level: BBa_K165053
start: 3112
end: 4605
orientation: inline

BBa_K165097
type: ComponentDefinition
persistent identity: BBa K165097
display id: BBa_K165097
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Gli1 bs + MET25+ LexA repressor (CFPx2 tagged) on pRS304*
creator: John Szymanski
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:10:57Z
discontinued: false
dominant: false
group u list: _267_
m user id: 0
owner id: 2512
owning group id: 58
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: -
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_K165018
component: BBa_K165014
component: BBa_K165002
component: BBa_K165012
component: BBa_K165009
component: BBa_K165000
component: BBa_K165003
sequence annotation: BBa_K165003
sequence annotation: BBa_K165002
sequence annotation: BBa_K165012
sequence annotation: BBa_K165000
sequence annotation: BBa_K165018
sequence annotation: BBa_K165009
sequence annotation: BBa_K165014
sequence: https://synbiohub.org/public/igem/BBa_K165097_sequence/1

BBa_K165018
type: Component
persistent identity: component1997373
display id: component1997373
version: 1
top level: BBa_K165097
access: public
definition: https://synbiohub.org/public/igem/BBa_K165018/1

BBa_K165014
type: Component
persistent identity: component1997372
display id: component1997372
version: 1
top level: BBa_K165097
access: public
definition: https://synbiohub.org/public/igem/BBa_K165014/1

BBa_K165002
type: Component
persistent identity: component1997369
display id: component1997369
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BBa_K165012
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BBa_K165000
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BBa_K165003
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type: SequenceAnnotation
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BBa_K165000
type: SequenceAnnotation
persistent identity: annotation1997368
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persistent identity: range1997368
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BBa_K165018
type: SequenceAnnotation
persistent identity: annotation1997373
display id: annotation1997373
version: 1
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location: https://synbiohub.org/public/igem/BBa_K165097/annotation1997373/range1997373/1
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persistent identity: range1997373
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BBa_K165009
type: SequenceAnnotation
persistent identity: annotation1997370
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persistent identity: range1997370
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BBa_K165014
type: SequenceAnnotation
persistent identity: annotation1997372
display id: annotation1997372
version: 1
top level: BBa_K165097
location: https://synbiohub.org/public/igem/BBa_K165097/annotation1997372/range1997372/1
component: BBa_K165014

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type: Range
persistent identity: range1997372
display id: range1997372
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start: 3642
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BBa_K1486040
type: ComponentDefinition
persistent identity: BBa K1486040
display id: BBa_K1486040
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: sfGFPN + CpxR
creator: EPFL iGem team 2014
created: 2014-09-26T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: To edit
mutable notes: To edit
mutable provenance: To edit
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_I0500
component: BBa_K1486038
component: BBa_K1486037
component: BBa_B0034
component: BBa_K1486000
sequence annotation: BBa_K1486038
sequence annotation: BBa_K1486037
sequence annotation: BBa_I0500
sequence annotation: BBa_K1486000
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K1486040_sequence/1

BBa_I0500
type: Component
persistent identity: component2387185
display id: component2387185
version: 1
top level: BBa_K1486040
access: public
definition: https://synbiohub.org/public/igem/BBa_I0500/1

BBa_K1486038
type: Component
persistent identity: component2387188
display id: component2387188
version: 1
top level: BBa_K1486040
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486038/1

BBa_K1486037
type: Component
persistent identity: component2387189
display id: component2387189
version: 1
top level: BBa_K1486040
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486037/1

BBa_B0034
type: Component
persistent identity: component2387187
display id: component2387187
version: 1
top level: BBa_K1486040
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K1486000
type: Component
persistent identity: component2387193
display id: component2387193
version: 1
top level: BBa_K1486040
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486000/1

BBa_K1486038
type: SequenceAnnotation
persistent identity: annotation2387188
display id: annotation2387188
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component: BBa_K1486038

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type: SequenceAnnotation
persistent identity: annotation2387189
display id: annotation2387189
version: 1
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type: SequenceAnnotation
persistent identity: annotation2387185
display id: annotation2387185
version: 1
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BBa_K1486000
type: SequenceAnnotation
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BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2387187
display id: annotation2387187
version: 1
top level: BBa_K1486040
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persistent identity: range2387187
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version: 1
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start: 1211
end: 1222
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BBa_K332021
type: ComponentDefinition
persistent identity: BBa K332021
display id: BBa_K332021
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Ptet + RBS + LuxR
creator: Chen-Hsi Tang
created: 2010-10-23T11:00:00Z
modified: 2015-05-08T01:12:05Z
discontinued: false
dominant: false
experience: experience/None
group u list: _452_
m user id: 0
owner id: 7483
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: In this portion of the design, we aim to achieve population control by combining a cell-signaling system (luxR/luxI) and suicidal gene for controlling the number of E.coli. The population controlling function in the engineered E.coli can warn us of high-density E. coli congregations by emitting fluorescent proteins. Green fluorescent light means the Cry circuit is functional and the population density is under control, while red fluorescent gene signals E.coli overpopulation.
mutable notes: (I) Strand C (BBa_ K332021) 1. Strand C is synthesized from three fragments ??? (1) Ptet (2) RBS+LuxR (3) RBS+LuxI+double ter sites. 2. All of them are in the form of plasmids, so we digest them by appropriate restriction enzymes. 3. We first combine the last two fragments by ligation. The procedure is as follows: (1)Add the respective digestion products, vector plasmid, buffer, ligase to ddwater (2)React at 16??C for 30 minutes (3)Inactivate the ligase by incubation it at 80??C for 20 minutes (4)Store the products at 4??C 4. The products of ligation are then transformed into competent cells. The procedure is as follows: (1)2??l plasmids(ligation products) are added to 33??l competent cells (2)Shake gently for one second, and cool it on ice (3)Incubate it in 42??C water bath for 45 seconds (4)Spread the liquid to plate which corresponding antibiotics have been added to (5)37??C overnight 5. The next day we do a colony PCR for the transformed cells and have the PCR products electrophoresis to make sure that the two fragments have been combined correctly. 6. Now we separate the plasmids(RBS+LuxR+RBS+LuxI+double ter sites) from E. Coli, and digest it with restriction enzyme. 7. The first fragment (Ptet) is then combined with the above product by ligation, and transformed into competent cells by the same we have done early. 8. Because the length of Ptet to the remain segment is relatively too short, we cannot confirm whether they have combined correctly by just do an electrophoresis of the colony PCR products. If the electrophoresis result seems like that they have combined, we then send the product to sequencing.
mutable provenance: 1. LuxI protein (BBa_C0061) is the synthase that converts S-adenosylmethionine (SAM) into acyl-homoserine lactone (AHL). When the concentration of AHL reaches a threshold level, it binds to the N-terminal domain of a LuxR protein. Then the LuxR/AHL complex binds the Lux box within PtetR promoter (BBa_C0040), which then activates suicidal gene expression. 2. LuxR (BBa_C0062) is transcribed continuously by the constitutive promoter (BBa_J23106), and later works in conjunction with AHL. This design can control the concentration of LuxR protein in a steady phase, and the concentration of AHL/LuxR complex is only affected by the concentration of LuxI protein (BBa_C0061).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_C0062
component: BBa_R0040
component: BBa_B0034
sequence annotation: BBa_R0040
sequence annotation: BBa_C0062
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K332021_sequence/1

BBa_C0062
type: Component
persistent identity: component2247626
display id: component2247626
version: 1
top level: BBa_K332021
access: public
definition: luxr

BBa_R0040
type: Component
persistent identity: component2247617
display id: component2247617
version: 1
top level: BBa_K332021
access: public
definition: https://synbiohub.org/public/igem/BBa_R0040/1

BBa_B0034
type: Component
persistent identity: component2247623
display id: component2247623
version: 1
top level: BBa_K332021
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation2247617
display id: annotation2247617
version: 1
top level: BBa_K332021
location: https://synbiohub.org/public/igem/BBa_K332021/annotation2247617/range2247617/1
component: BBa_R0040

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type: Range
persistent identity: range2247617
display id: range2247617
version: 1
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start: 1
end: 54
orientation: inline

BBa_C0062
type: SequenceAnnotation
persistent identity: annotation2247626
display id: annotation2247626
version: 1
top level: BBa_K332021
location: https://synbiohub.org/public/igem/BBa_K332021/annotation2247626/range2247626/1
component: BBa_C0062

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type: Range
persistent identity: range2247626
display id: range2247626
version: 1
direction: direction/forward
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start: 81
end: 836
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2247623
display id: annotation2247623
version: 1
top level: BBa_K332021
location: https://synbiohub.org/public/igem/BBa_K332021/annotation2247623/range2247623/1
component: BBa_B0034

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type: Range
persistent identity: range2247623
display id: range2247623
version: 1
direction: direction/reverse
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end: 74
orientation: inline

LuxR
type: ComponentDefinition
persistent identity: BBa I0462
display id: BBa_I0462
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: luxR Protein Generator
creator: Caitlin Conboy and Jennifer Braff
created: 2003-12-04T12:00:00Z
modified: 2015-08-31T04:07:29Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Produces LuxR protein which can sense 3OC6HSL in the media and activate transcription from R0062, the right hand Lux promoter
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_C0062
component: BBa_B0034
component: BBa_B0010
component: BBa_B0012
sequence annotation: BBa_B0012
sequence annotation: BBa_B0010
sequence annotation: BBa_B0034
sequence annotation: BBa_C0062
sequence: https://synbiohub.org/public/igem/BBa_I0462_sequence/1

BBa_C0062
type: Component
persistent identity: component943181
display id: component943181
version: 1
top level: LuxR
access: public
definition: luxr

BBa_B0034
type: Component
persistent identity: component943166
display id: component943166
version: 1
top level: LuxR
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0010
type: Component
persistent identity: component943189
display id: component943189
version: 1
top level: LuxR
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0012
type: Component
persistent identity: component943199
display id: component943199
version: 1
top level: LuxR
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation943199
display id: annotation943199
version: 1
top level: LuxR
location: https://synbiohub.org/public/igem/BBa_I0462/annotation943199/range943199/1
component: BBa_B0012

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type: Range
persistent identity: range943199
display id: range943199
version: 1
direction: direction/forward
top level: LuxR
start: 896
end: 936
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation943189
display id: annotation943189
version: 1
top level: LuxR
location: https://synbiohub.org/public/igem/BBa_I0462/annotation943189/range943189/1
component: BBa_B0010

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type: Range
persistent identity: range943189
display id: range943189
version: 1
direction: direction/forward
top level: LuxR
start: 808
end: 887
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation943166
display id: annotation943166
version: 1
top level: LuxR
location: https://synbiohub.org/public/igem/BBa_I0462/annotation943166/range943166/1
component: BBa_B0034

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type: Range
persistent identity: range943166
display id: range943166
version: 1
direction: direction/forward
top level: LuxR
start: 1
end: 12
orientation: inline

BBa_C0062
type: SequenceAnnotation
persistent identity: annotation943181
display id: annotation943181
version: 1
top level: LuxR
location: https://synbiohub.org/public/igem/BBa_I0462/annotation943181/range943181/1
component: BBa_C0062

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type: Range
persistent identity: range943181
display id: range943181
version: 1
direction: direction/forward
top level: LuxR
start: 19
end: 774
orientation: inline

BBa_K100010
type: ComponentDefinition
persistent identity: BBa K100010
display id: BBa_K100010
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS + XylR Transcriptional Regulator
creator: Garrett Tobin
created: 2008-08-17T11:00:00Z
modified: 2015-05-08T01:08:41Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _213_
m user id: 0
owner id: 1752
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: RBS (BBa_B0030) placed in front of the ORF of XylR Transcriptional Regulator (BBa_I741005)
mutable notes: B0030 was used because of its stregnth, planning on expressing high levels of XylR
mutable provenance: Composite - See BBa_B0030 and BBa_I741005
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_B0030
component: BBa_I741005
sequence annotation: BBa_B0030
sequence annotation: BBa_I741005
sequence: https://synbiohub.org/public/igem/BBa_K100010_sequence/1

BBa_B0030
type: Component
persistent identity: component1972248
display id: component1972248
version: 1
top level: BBa_K100010
access: public
definition: https://synbiohub.org/public/igem/BBa_B0030/1

BBa_I741005
type: Component
persistent identity: component1972253
display id: component1972253
version: 1
top level: BBa_K100010
access: public
definition: https://synbiohub.org/public/igem/BBa_I741005/1

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation1972248
display id: annotation1972248
version: 1
top level: BBa_K100010
location: https://synbiohub.org/public/igem/BBa_K100010/annotation1972248/range1972248/1
component: BBa_B0030

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type: Range
persistent identity: range1972248
display id: range1972248
version: 1
direction: direction/forward
top level: BBa_K100010
start: 1
end: 15
orientation: inline

BBa_I741005
type: SequenceAnnotation
persistent identity: annotation1972253
display id: annotation1972253
version: 1
top level: BBa_K100010
location: https://synbiohub.org/public/igem/BBa_K100010/annotation1972253/range1972253/1
component: BBa_I741005

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type: Range
persistent identity: range1972253
display id: range1972253
version: 1
direction: direction/reverse
top level: BBa_K100010
start: 22
end: 1200
orientation: inline

BBa_K283024
type: ComponentDefinition
persistent identity: BBa K283024
display id: BBa_K283024
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pTet-rbs-SsrB
creator: Shi Lei, W Ling , Nelson Chu
created: 2009-10-12T11:00:00Z
modified: 2015-05-08T01:11:47Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _383_
m user id: 0
owner id: 5494
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: ssrB protein under pTet promoter. The ssrB protein can activate the prosseA promoter. The pTet promoter can be repressed by TetR repressor protein. The ssrB coding region does not contain any terminator
mutable notes: BBa_K283024
mutable provenance: The ssrB coding region is self-designed The rbs and the pTet promoter are from the registry
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: -35
sequence annotation: -10
sequence annotation: conserved
sequence annotation: ssrB
sequence annotation: BBa_B0034
sequence annotation: BBa_R0040
sequence annotation: TetR 1
sequence annotation: TetR 2
sequence: https://synbiohub.org/public/igem/BBa_K283024_sequence/1

-35
type: SequenceAnnotation
persistent identity: annotation2035712
display id: annotation2035712
version: 1
top level: BBa_K283024
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K283024/annotation2035712/range2035712/1

https://synbiohub.org/public/igem/BBa_K283024/annotation2035712/range2035712/1
type: Range
persistent identity: range2035712
display id: range2035712
version: 1
direction: direction/forward
top level: BBa_K283024
start: 20
end: 25
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2035714
display id: annotation2035714
version: 1
top level: BBa_K283024
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K283024/annotation2035714/range2035714/1

https://synbiohub.org/public/igem/BBa_K283024/annotation2035714/range2035714/1
type: Range
persistent identity: range2035714
display id: range2035714
version: 1
direction: direction/forward
top level: BBa_K283024
start: 43
end: 48
orientation: inline

conserved
type: SequenceAnnotation
persistent identity: annotation2035715
display id: annotation2035715
version: 1
top level: BBa_K283024
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K283024/annotation2035715/range2035715/1

https://synbiohub.org/public/igem/BBa_K283024/annotation2035715/range2035715/1
type: Range
persistent identity: range2035715
display id: range2035715
version: 1
direction: direction/forward
top level: BBa_K283024
start: 67
end: 70
orientation: inline

ssrB
type: SequenceAnnotation
persistent identity: annotation2040403
display id: annotation2040403
version: 1
top level: BBa_K283024
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K283024/annotation2040403/range2040403/1

https://synbiohub.org/public/igem/BBa_K283024/annotation2040403/range2040403/1
type: Range
persistent identity: range2040403
display id: range2040403
version: 1
direction: direction/forward
top level: BBa_K283024
start: 75
end: 702
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2035716
display id: annotation2035716
version: 1
top level: BBa_K283024
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K283024/annotation2035716/range2035716/1

https://synbiohub.org/public/igem/BBa_K283024/annotation2035716/range2035716/1
type: Range
persistent identity: range2035716
display id: range2035716
version: 1
direction: direction/reverse
top level: BBa_K283024
start: 63
end: 74
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation2035710
display id: annotation2035710
version: 1
top level: BBa_K283024
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K283024/annotation2035710/range2035710/1

https://synbiohub.org/public/igem/BBa_K283024/annotation2035710/range2035710/1
type: Range
persistent identity: range2035710
display id: range2035710
version: 1
direction: direction/forward
top level: BBa_K283024
start: 1
end: 54
orientation: inline

TetR 1
type: SequenceAnnotation
persistent identity: annotation2035711
display id: annotation2035711
version: 1
top level: BBa_K283024
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K283024/annotation2035711/range2035711/1

https://synbiohub.org/public/igem/BBa_K283024/annotation2035711/range2035711/1
type: Range
persistent identity: range2035711
display id: range2035711
version: 1
direction: direction/forward
top level: BBa_K283024
start: 1
end: 19
orientation: inline

TetR 2
type: SequenceAnnotation
persistent identity: annotation2035713
display id: annotation2035713
version: 1
top level: BBa_K283024
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K283024/annotation2035713/range2035713/1

https://synbiohub.org/public/igem/BBa_K283024/annotation2035713/range2035713/1
type: Range
persistent identity: range2035713
display id: range2035713
version: 1
direction: direction/forward
top level: BBa_K283024
start: 26
end: 44
orientation: inline

pArsR
type: ComponentDefinition
persistent identity: BBa K190015
display id: BBa_K190015
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Arsenic Promoter (ArsR regulated)
creator: Michael Verhoeven
created: 2009-07-14T11:00:00Z
modified: 2015-05-08T01:11:15Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _306_
m user id: 0
owner id: 4144
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: Promoter sequence with recognition site for ArsR transcriptional regulator protein. ArsR binds to the promoter sequence in the absence of As and releases on binding of As, therby activating transcription.
mutable notes: None
mutable provenance: E.coli TOP10
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: ArsR binding site
sequence annotation: -35 and -10 region
sequence: https://synbiohub.org/public/igem/BBa_K190015_sequence/1

ArsR binding site
type: SequenceAnnotation
persistent identity: annotation2011501
display id: annotation2011501
version: 1
top level: pArsR
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K190015/annotation2011501/range2011501/1

https://synbiohub.org/public/igem/BBa_K190015/annotation2011501/range2011501/1
type: Range
persistent identity: range2011501
display id: range2011501
version: 1
direction: direction/forward
top level: pArsR
start: 9
end: 41
orientation: inline

-35 and -10 region
type: SequenceAnnotation
persistent identity: annotation2011502
display id: annotation2011502
version: 1
top level: pArsR
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K190015/annotation2011502/range2011502/1

https://synbiohub.org/public/igem/BBa_K190015/annotation2011502/range2011502/1
type: Range
persistent identity: range2011502
display id: range2011502
version: 1
direction: direction/forward
top level: pArsR
start: 38
end: 72
orientation: inline

BBa_K1520006
type: ComponentDefinition
persistent identity: BBa K1520006
display id: BBa_K1520006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Prlux-rbs-cI-Ter
creator: yue wu
created: 2014-09-21T11:00:00Z
modified: 2015-05-08T01:10:48Z
discontinued: false
dominant: false
group u list: _1902_
m user id: 0
owner id: 21318
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Prlux-rbs-cI-Ter. It produces cI when there is enough luxR and AHL.
mutable notes: None
mutable provenance: Prlux, cI, Ter
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_R0062
component: BBa_K1520503
component: BBa_K1520029
sequence annotation: BBa_K1520503
sequence annotation: BBa_R0062
sequence annotation: BBa_K1520029
sequence: https://synbiohub.org/public/igem/BBa_K1520006_sequence/1

BBa_R0062
type: Component
persistent identity: component2384911
display id: component2384911
version: 1
top level: BBa_K1520006
access: public
definition: https://synbiohub.org/public/igem/BBa_R0062/1

BBa_K1520503
type: Component
persistent identity: component2384918
display id: component2384918
version: 1
top level: BBa_K1520006
access: public
definition: BBa_K1520503

BBa_K1520029
type: Component
persistent identity: component2384919
display id: component2384919
version: 1
top level: BBa_K1520006
access: public
definition: https://synbiohub.org/public/igem/BBa_K1520029/1

BBa_K1520503
type: SequenceAnnotation
persistent identity: annotation2384918
display id: annotation2384918
version: 1
top level: BBa_K1520006
location: https://synbiohub.org/public/igem/BBa_K1520006/annotation2384918/range2384918/1
component: BBa_K1520503

https://synbiohub.org/public/igem/BBa_K1520006/annotation2384918/range2384918/1
type: Range
persistent identity: range2384918
display id: range2384918
version: 1
direction: direction/reverse
top level: BBa_K1520006
start: 64
end: 856
orientation: inline

BBa_R0062
type: SequenceAnnotation
persistent identity: annotation2384911
display id: annotation2384911
version: 1
top level: BBa_K1520006
location: https://synbiohub.org/public/igem/BBa_K1520006/annotation2384911/range2384911/1
component: BBa_R0062

https://synbiohub.org/public/igem/BBa_K1520006/annotation2384911/range2384911/1
type: Range
persistent identity: range2384911
display id: range2384911
version: 1
direction: direction/forward
top level: BBa_K1520006
start: 1
end: 55
orientation: inline

BBa_K1520029
type: SequenceAnnotation
persistent identity: annotation2384919
display id: annotation2384919
version: 1
top level: BBa_K1520006
location: https://synbiohub.org/public/igem/BBa_K1520006/annotation2384919/range2384919/1
component: BBa_K1520029

https://synbiohub.org/public/igem/BBa_K1520006/annotation2384919/range2384919/1
type: Range
persistent identity: range2384919
display id: range2384919
version: 1
direction: direction/reverse
top level: BBa_K1520006
start: 865
end: 993
orientation: inline

BBa_K1520514
type: ComponentDefinition
persistent identity: BBa K1520514
display id: BBa_K1520514
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: MC31-rbs-tetR-Ter-PtetO-rbs-rfp-Ter-Plac-rbs-tetR-Ter-Pcons2-rbs-lacI-Ter
creator: yue wu
created: 2014-09-22T11:00:00Z
modified: 2015-05-08T01:10:49Z
discontinued: false
dominant: false
group u list: _1902_
m user id: 0
owner id: 21318
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: MC31-rbs-tetR-Ter-PtetO-rbs-rfp-Ter-Plac-rbs-tetR-Ter-Pcons2-rbs-lacI-Ter It is a test of our suicide circuit for microcystin.
mutable notes: None
mutable provenance: MC31, tetR, PtetO, rfp, Plac, Pcons2, lacI, Ter
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1520505
component: BBa_K1520026
component: BBa_K1520002
sequence annotation: BBa_K1520002
sequence annotation: BBa_K1520026
sequence annotation: BBa_K1520505
sequence: https://synbiohub.org/public/igem/BBa_K1520514_sequence/1

BBa_K1520505
type: Component
persistent identity: component2385636
display id: component2385636
version: 1
top level: BBa_K1520514
access: public
definition: BBa_K1520505

BBa_K1520026
type: Component
persistent identity: component2385664
display id: component2385664
version: 1
top level: BBa_K1520514
access: public
definition: https://synbiohub.org/public/igem/BBa_K1520026/1

BBa_K1520002
type: Component
persistent identity: component2385633
display id: component2385633
version: 1
top level: BBa_K1520514
access: public
definition: https://synbiohub.org/public/igem/BBa_K1520002/1

BBa_K1520002
type: SequenceAnnotation
persistent identity: annotation2385633
display id: annotation2385633
version: 1
top level: BBa_K1520514
location: https://synbiohub.org/public/igem/BBa_K1520514/annotation2385633/range2385633/1
component: BBa_K1520002

https://synbiohub.org/public/igem/BBa_K1520514/annotation2385633/range2385633/1
type: Range
persistent identity: range2385633
display id: range2385633
version: 1
direction: direction/reverse
top level: BBa_K1520514
start: 1
end: 132
orientation: inline

BBa_K1520026
type: SequenceAnnotation
persistent identity: annotation2385664
display id: annotation2385664
version: 1
top level: BBa_K1520514
location: https://synbiohub.org/public/igem/BBa_K1520514/annotation2385664/range2385664/1
component: BBa_K1520026

https://synbiohub.org/public/igem/BBa_K1520514/annotation2385664/range2385664/1
type: Range
persistent identity: range2385664
display id: range2385664
version: 1
direction: direction/reverse
top level: BBa_K1520514
start: 852
end: 4197
orientation: inline

BBa_K1520505
type: SequenceAnnotation
persistent identity: annotation2385636
display id: annotation2385636
version: 1
top level: BBa_K1520514
location: https://synbiohub.org/public/igem/BBa_K1520514/annotation2385636/range2385636/1
component: BBa_K1520505

https://synbiohub.org/public/igem/BBa_K1520514/annotation2385636/range2385636/1
type: Range
persistent identity: range2385636
display id: range2385636
version: 1
direction: direction/reverse
top level: BBa_K1520514
start: 141
end: 843
orientation: inline

BBa_I726061
type: ComponentDefinition
persistent identity: BBa I726061
display id: BBa_I726061
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Trc-LRY
creator: Bangalore iGEM
created: 2007-07-03T11:00:00Z
modified: 2015-08-31T04:07:55Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _107_
m user id: 0
owner id: 2077
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Abcdefghijkl..
mutable notes: none
mutable provenance: Registry
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_B0034
component: BBa_B0012
component: BBa_C0062
component: BBa_B0010
component: BBa_R0011
component: BBa_E0030
sequence annotation: BBa_B0012
sequence annotation: BBa_C0062
sequence annotation: BBa_B0034
sequence annotation: BBa_R0011
sequence annotation: BBa_B0010
sequence annotation: BBa_E0030
sequence: https://synbiohub.org/public/igem/BBa_I726061_sequence/1

BBa_B0034
type: Component
persistent identity: component2265602
display id: component2265602
version: 1
top level: BBa_I726061
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0012
type: Component
persistent identity: component2265606
display id: component2265606
version: 1
top level: BBa_I726061
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_C0062
type: Component
persistent identity: component2265597
display id: component2265597
version: 1
top level: BBa_I726061
access: public
definition: luxr

BBa_B0010
type: Component
persistent identity: component2265604
display id: component2265604
version: 1
top level: BBa_I726061
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_R0011
type: Component
persistent identity: component2265588
display id: component2265588
version: 1
top level: BBa_I726061
access: public
definition: https://synbiohub.org/public/igem/BBa_R0011/1

BBa_E0030
type: Component
persistent identity: component2265603
display id: component2265603
version: 1
top level: BBa_I726061
access: public
definition: https://synbiohub.org/public/igem/BBa_E0030/1

BBa_B0034
type: Component
persistent identity: component2265594
display id: component2265594
version: 1
top level: BBa_I726061
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2265606
display id: annotation2265606
version: 1
top level: BBa_I726061
location: https://synbiohub.org/public/igem/BBa_I726061/annotation2265606/range2265606/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I726061/annotation2265606/range2265606/1
type: Range
persistent identity: range2265606
display id: range2265606
version: 1
direction: direction/forward
top level: BBa_I726061
start: 1708
end: 1748
orientation: inline

BBa_C0062
type: SequenceAnnotation
persistent identity: annotation2265597
display id: annotation2265597
version: 1
top level: BBa_I726061
location: https://synbiohub.org/public/igem/BBa_I726061/annotation2265597/range2265597/1
component: BBa_C0062

https://synbiohub.org/public/igem/BBa_I726061/annotation2265597/range2265597/1
type: Range
persistent identity: range2265597
display id: range2265597
version: 1
direction: direction/forward
top level: BBa_I726061
start: 82
end: 837
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2265602
display id: annotation2265602
version: 1
top level: BBa_I726061
location: https://synbiohub.org/public/igem/BBa_I726061/annotation2265602/range2265602/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I726061/annotation2265602/range2265602/1
type: Range
persistent identity: range2265602
display id: range2265602
version: 1
direction: direction/reverse
top level: BBa_I726061
start: 871
end: 882
orientation: inline

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation2265588
display id: annotation2265588
version: 1
top level: BBa_I726061
location: https://synbiohub.org/public/igem/BBa_I726061/annotation2265588/range2265588/1
component: BBa_R0011

https://synbiohub.org/public/igem/BBa_I726061/annotation2265588/range2265588/1
type: Range
persistent identity: range2265588
display id: range2265588
version: 1
direction: direction/forward
top level: BBa_I726061
start: 1
end: 54
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2265594
display id: annotation2265594
version: 1
top level: BBa_I726061
location: https://synbiohub.org/public/igem/BBa_I726061/annotation2265594/range2265594/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I726061/annotation2265594/range2265594/1
type: Range
persistent identity: range2265594
display id: range2265594
version: 1
direction: direction/reverse
top level: BBa_I726061
start: 64
end: 75
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2265604
display id: annotation2265604
version: 1
top level: BBa_I726061
location: https://synbiohub.org/public/igem/BBa_I726061/annotation2265604/range2265604/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I726061/annotation2265604/range2265604/1
type: Range
persistent identity: range2265604
display id: range2265604
version: 1
direction: direction/forward
top level: BBa_I726061
start: 1620
end: 1699
orientation: inline

BBa_E0030
type: SequenceAnnotation
persistent identity: annotation2265603
display id: annotation2265603
version: 1
top level: BBa_I726061
location: https://synbiohub.org/public/igem/BBa_I726061/annotation2265603/range2265603/1
component: BBa_E0030

https://synbiohub.org/public/igem/BBa_I726061/annotation2265603/range2265603/1
type: Range
persistent identity: range2265603
display id: range2265603
version: 1
direction: direction/reverse
top level: BBa_I726061
start: 889
end: 1611
orientation: inline

Fur
type: ComponentDefinition
persistent identity: BBa K1122666
display id: BBa_K1122666
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Ferric uptake repressor
creator: Hugo Villanueva
created: 2013-07-23T11:00:00Z
modified: 2015-07-24T01:14:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1433_
m user id: 4206
owner id: 18022
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: The ferric uptake repressor of Bacillus subtilis called YqkL is a regulator that when in contact with iron will bind and repress a regulatory sequence (the Fur box).
mutable notes: We sequenced the Fur box. This can be added in front of genes to provide regulation when in contact with iron.
mutable provenance: This comes from Bacillus subtilis.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1122666_sequence/1

BBa_K190016
type: ComponentDefinition
persistent identity: BBa K190016
display id: BBa_K190016
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Zinc Promoter (ZntR regulated)
creator: Michael Verhoeven
created: 2009-07-14T11:00:00Z
modified: 2015-05-08T01:11:15Z
discontinued: false
dominant: false
experience: experience/Fails
group u list: _306_
m user id: 0
owner id: 4144
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Promoter sequence with recognition site for ZntR transcriptional regulator protein.
mutable notes: None
mutable provenance: E.coli TOP10
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -35 and -10 region
sequence annotation: Palindromic binding site for ZntR
sequence: https://synbiohub.org/public/igem/BBa_K190016_sequence/1

-35 and -10 region
type: SequenceAnnotation
persistent identity: annotation2011503
display id: annotation2011503
version: 1
top level: BBa_K190016
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K190016/annotation2011503/range2011503/1

https://synbiohub.org/public/igem/BBa_K190016/annotation2011503/range2011503/1
type: Range
persistent identity: range2011503
display id: range2011503
version: 1
direction: direction/forward
top level: BBa_K190016
start: 35
end: 69
orientation: inline

Palindromic binding site for ZntR
type: SequenceAnnotation
persistent identity: annotation2011504
display id: annotation2011504
version: 1
top level: BBa_K190016
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K190016/annotation2011504/range2011504/1

https://synbiohub.org/public/igem/BBa_K190016/annotation2011504/range2011504/1
type: Range
persistent identity: range2011504
display id: range2011504
version: 1
direction: direction/forward
top level: BBa_K190016
start: 32
end: 53
orientation: inline

BBa_P0140
type: ComponentDefinition
persistent identity: BBa P0140
display id: BBa_P0140
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PoPS -> TetR [S0163]
creator: Randy Rettberg
created: 2004-04-24T11:00:00Z
modified: 2015-05-08T01:14:10Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Protein generator converting TIPS to the protein TetR. Used as the input section for Quad Part Inverter Q01140.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0010
component: BBa_C0040
component: BBa_B0012
component: BBa_B0031
sequence annotation: BBa_B0012
sequence annotation: BBa_B0010
sequence annotation: BBa_B0031
sequence annotation: BBa_C0040
sequence: https://synbiohub.org/public/igem/BBa_P0140_sequence/1

BBa_B0010
type: Component
persistent identity: component944448
display id: component944448
version: 1
top level: BBa_P0140
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_C0040
type: Component
persistent identity: component944442
display id: component944442
version: 1
top level: BBa_P0140
access: public
definition: tetR

BBa_B0012
type: Component
persistent identity: component944458
display id: component944458
version: 1
top level: BBa_P0140
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0031
type: Component
persistent identity: component944432
display id: component944432
version: 1
top level: BBa_P0140
access: public
definition: https://synbiohub.org/public/igem/BBa_B0031/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation944458
display id: annotation944458
version: 1
top level: BBa_P0140
location: https://synbiohub.org/public/igem/BBa_P0140/annotation944458/range944458/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_P0140/annotation944458/range944458/1
type: Range
persistent identity: range944458
display id: range944458
version: 1
direction: direction/forward
top level: BBa_P0140
start: 802
end: 842
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation944448
display id: annotation944448
version: 1
top level: BBa_P0140
location: https://synbiohub.org/public/igem/BBa_P0140/annotation944448/range944448/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_P0140/annotation944448/range944448/1
type: Range
persistent identity: range944448
display id: range944448
version: 1
direction: direction/forward
top level: BBa_P0140
start: 714
end: 793
orientation: inline

BBa_B0031
type: SequenceAnnotation
persistent identity: annotation944432
display id: annotation944432
version: 1
top level: BBa_P0140
location: https://synbiohub.org/public/igem/BBa_P0140/annotation944432/range944432/1
component: BBa_B0031

https://synbiohub.org/public/igem/BBa_P0140/annotation944432/range944432/1
type: Range
persistent identity: range944432
display id: range944432
version: 1
direction: direction/forward
top level: BBa_P0140
start: 1
end: 14
orientation: inline

BBa_C0040
type: SequenceAnnotation
persistent identity: annotation944442
display id: annotation944442
version: 1
top level: BBa_P0140
location: https://synbiohub.org/public/igem/BBa_P0140/annotation944442/range944442/1
component: BBa_C0040

https://synbiohub.org/public/igem/BBa_P0140/annotation944442/range944442/1
type: Range
persistent identity: range944442
display id: range944442
version: 1
direction: direction/forward
top level: BBa_P0140
start: 21
end: 680
orientation: inline

BBa_J07009
type: ComponentDefinition
persistent identity: BBa J07009
display id: BBa_J07009
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: toxicity-gene activator from Vibrio cholerae
creator: mit_igem_2005
created: 2005-07-06T11:00:00Z
modified: 2015-08-31T04:08:19Z
discontinued: false
dominant: false
experience: experience/None
group u list: _21_
m user id: 0
owner id: 363
owning group id: 21
sample status: In stock
status: status/Available
bookmark: true
mutable description: shortened BBa_J07008. Typical ToxR-periplasm-region subsitutions are done @ aa 211. explanation of ToxR domains @ http://model.mit.edu/igem/images/d/d7/Kolmar95.pdf ELIMINATE XbaI SITE PLZZZZZ
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Cytoplasmic Region
sequence annotation: Start Codon
sequence annotation: Periplasmic Region
sequence annotation: Coding Sequence for ToxR'
sequence annotation: Transmembrane Region
sequence: https://synbiohub.org/public/igem/BBa_J07009_sequence/1

Cytoplasmic Region
type: SequenceAnnotation
persistent identity: annotation1668506
display id: annotation1668506
version: 1
top level: BBa_J07009
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J07009/annotation1668506/range1668506/1

https://synbiohub.org/public/igem/BBa_J07009/annotation1668506/range1668506/1
type: Range
persistent identity: range1668506
display id: range1668506
version: 1
direction: direction/forward
top level: BBa_J07009
start: 1
end: 546
orientation: inline

Start Codon
type: SequenceAnnotation
persistent identity: annotation1668508
display id: annotation1668508
version: 1
top level: BBa_J07009
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_J07009/annotation1668508/range1668508/1

https://synbiohub.org/public/igem/BBa_J07009/annotation1668508/range1668508/1
type: Range
persistent identity: range1668508
display id: range1668508
version: 1
direction: direction/forward
top level: BBa_J07009
start: 6
end: 8
orientation: inline

Periplasmic Region
type: SequenceAnnotation
persistent identity: annotation1668510
display id: annotation1668510
version: 1
top level: BBa_J07009
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J07009/annotation1668510/range1668510/1

https://synbiohub.org/public/igem/BBa_J07009/annotation1668510/range1668510/1
type: Range
persistent identity: range1668510
display id: range1668510
version: 1
direction: direction/forward
top level: BBa_J07009
start: 595
end: 630
orientation: inline

Coding Sequence for ToxR'
type: SequenceAnnotation
persistent identity: annotation1668507
display id: annotation1668507
version: 1
top level: BBa_J07009
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J07009/annotation1668507/range1668507/1

https://synbiohub.org/public/igem/BBa_J07009/annotation1668507/range1668507/1
type: Range
persistent identity: range1668507
display id: range1668507
version: 1
direction: direction/forward
top level: BBa_J07009
start: 1
end: 630
orientation: inline

Transmembrane Region
type: SequenceAnnotation
persistent identity: annotation1668509
display id: annotation1668509
version: 1
top level: BBa_J07009
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J07009/annotation1668509/range1668509/1

https://synbiohub.org/public/igem/BBa_J07009/annotation1668509/range1668509/1
type: Range
persistent identity: range1668509
display id: range1668509
version: 1
direction: direction/forward
top level: BBa_J07009
start: 547
end: 594
orientation: inline

BBa_K292005
type: ComponentDefinition
persistent identity: BBa K292005
display id: BBa_K292005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LacI repressor and a double terminator
creator: David Charoy
created: 2009-10-18T11:00:00Z
modified: 2015-05-08T01:11:49Z
discontinued: false
dominant: false
group u list: _394_
m user id: 0
owner id: 5212
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part contains a LacI repressor and a terminator (BBa_C0012 + BBa_B0014). The LacI repressor repressed pLac promoter from the E.coli lactose operon. So LacI repressor is really reliable to use when we want to control a gene expression by using pLac promoter. In fact when LacI is not expressed, pLac is activated, and when there is an expression of LacI, pLac is inhibited. We can easily design a system to induce the transcription of LacI and creates a failed feedback mechanism for pLac inhibition. History: In E.Coli, pLac is a part of the lactose operon. LacI is constantly expressed and represses the pLac. In the presence of Lactose, LacI is inhibited and pLac is active and allows the transcription of beta-galactosidase. Lactose can be substituted by IPTG (Isopropyl β-D-1-thiogalactopyranoside).
mutable notes: When we design the bio-brick it was impossible to obtain a functional bio-brick by using the plasmid SB1AK3. But it's worked with a pSB1A2.
mutable provenance: Part:BBa_C0012, Designed by Grace Kenney, Daniel Shen, Neelaksh Varshney, Samantha Sutton Group: Registry Part:BBa_B0014, Designed by Reshma Shetty Group: Registry (2003-07-16).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_C0012
component: BBa_B0014
sequence annotation: BBa_C0012
sequence annotation: BBa_B0014
sequence: https://synbiohub.org/public/igem/BBa_K292005_sequence/1

BBa_C0012
type: Component
persistent identity: component2246883
display id: component2246883
version: 1
top level: BBa_K292005
access: public
definition: lacI

BBa_B0014
type: Component
persistent identity: component2246893
display id: component2246893
version: 1
top level: BBa_K292005
access: public
definition: https://synbiohub.org/public/igem/BBa_B0014/1

BBa_C0012
type: SequenceAnnotation
persistent identity: annotation2246883
display id: annotation2246883
version: 1
top level: BBa_K292005
location: https://synbiohub.org/public/igem/BBa_K292005/annotation2246883/range2246883/1
component: BBa_C0012

https://synbiohub.org/public/igem/BBa_K292005/annotation2246883/range2246883/1
type: Range
persistent identity: range2246883
display id: range2246883
version: 1
direction: direction/forward
top level: BBa_K292005
start: 1
end: 1128
orientation: inline

BBa_B0014
type: SequenceAnnotation
persistent identity: annotation2246893
display id: annotation2246893
version: 1
top level: BBa_K292005
location: https://synbiohub.org/public/igem/BBa_K292005/annotation2246893/range2246893/1
component: BBa_B0014

https://synbiohub.org/public/igem/BBa_K292005/annotation2246893/range2246893/1
type: Range
persistent identity: range2246893
display id: range2246893
version: 1
direction: direction/reverse
top level: BBa_K292005
start: 1162
end: 1256
orientation: inline

BBa_K544012
type: ComponentDefinition
persistent identity: BBa K544012
display id: BBa_K544012
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Tetracycline Repressor with Consitutive Promoter BBa_J23106
creator: Leung Man Hong
created: 2011-09-29T11:00:00Z
modified: 2015-05-08T01:12:39Z
discontinued: false
dominant: false
group u list: _711_
m user id: 0
owner id: 6780
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: A transcriptional regulator TetR (BBa_C0040) is driven by constitutive promoter family members (BBa-J23103, BBa-J23109, BBa-J23116, and BBa-J23106), which can be used to tune the expression level of constitutively expressed TetR.
mutable notes: The SsrA site at the end of BBa_C0040 is not present in our design.
mutable provenance: The source of this part is based on the parts in the registry distribution
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: BBa_B0034
sequence annotation: BBa_J23106
sequence annotation: tet-R
sequence: https://synbiohub.org/public/igem/BBa_K544012_sequence/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2148190
display id: annotation2148190
version: 1
top level: BBa_K544012
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K544012/annotation2148190/range2148190/1

https://synbiohub.org/public/igem/BBa_K544012/annotation2148190/range2148190/1
type: Range
persistent identity: range2148190
display id: range2148190
version: 1
direction: direction/forward
top level: BBa_K544012
start: 44
end: 55
orientation: inline

BBa_J23106
type: SequenceAnnotation
persistent identity: annotation2148189
display id: annotation2148189
version: 1
top level: BBa_K544012
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K544012/annotation2148189/range2148189/1

https://synbiohub.org/public/igem/BBa_K544012/annotation2148189/range2148189/1
type: Range
persistent identity: range2148189
display id: range2148189
version: 1
direction: direction/forward
top level: BBa_K544012
start: 1
end: 35
orientation: inline

tet-R
type: SequenceAnnotation
persistent identity: annotation2148191
display id: annotation2148191
version: 1
top level: BBa_K544012
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K544012/annotation2148191/range2148191/1

https://synbiohub.org/public/igem/BBa_K544012/annotation2148191/range2148191/1
type: Range
persistent identity: range2148191
display id: range2148191
version: 1
direction: direction/forward
top level: BBa_K544012
start: 62
end: 682
orientation: inline

BBa_K142075
type: ComponentDefinition
persistent identity: BBa K142075
display id: BBa_K142075
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: lac-controlled CFP generator with constitutive lacI expression cassette
creator: Julius Rabl
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:10:22Z
discontinued: false
dominant: false
group u list: _194_
m user id: 0
owner id: 3254
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: lac-controlled CFP generator with constitutive lacI expression cassette
mutable notes: We put the LacI generator behind the lac-controlled expression cassette to minimze readthrough.
mutable provenance: Subcloned from BioBricks using standard molecular biology techniques.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_I13601
component: BBa_I739002
sequence annotation: BBa_I739002
sequence annotation: BBa_I13601
sequence: https://synbiohub.org/public/igem/BBa_K142075_sequence/1

BBa_I13601
type: Component
persistent identity: component2283026
display id: component2283026
version: 1
top level: BBa_K142075
access: public
definition: https://synbiohub.org/public/igem/BBa_I13601/1

BBa_I739002
type: Component
persistent identity: component2283041
display id: component2283041
version: 1
top level: BBa_K142075
access: public
definition: https://synbiohub.org/public/igem/BBa_I739002/1

BBa_I739002
type: SequenceAnnotation
persistent identity: annotation2283041
display id: annotation2283041
version: 1
top level: BBa_K142075
location: https://synbiohub.org/public/igem/BBa_K142075/annotation2283041/range2283041/1
component: BBa_I739002

https://synbiohub.org/public/igem/BBa_K142075/annotation2283041/range2283041/1
type: Range
persistent identity: range2283041
display id: range2283041
version: 1
direction: direction/reverse
top level: BBa_K142075
start: 950
end: 2300
orientation: inline

BBa_I13601
type: SequenceAnnotation
persistent identity: annotation2283026
display id: annotation2283026
version: 1
top level: BBa_K142075
location: https://synbiohub.org/public/igem/BBa_K142075/annotation2283026/range2283026/1
component: BBa_I13601

https://synbiohub.org/public/igem/BBa_K142075/annotation2283026/range2283026/1
type: Range
persistent identity: range2283026
display id: range2283026
version: 1
direction: direction/reverse
top level: BBa_K142075
start: 1
end: 941
orientation: inline

BBa_K864200
type: ComponentDefinition
persistent identity: BBa K864200
display id: BBa_K864200
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: MarR native E. coli repressor
creator: Erik Lundin
created: 2012-09-24T11:00:00Z
modified: 2015-05-08T01:13:37Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1124_
m user id: 0
owner id: 9827
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: The marR gene encodes a repressor of the marRAB operon
mutable notes: None
mutable provenance: Amplified from MG1655 (Accession NC_000913) chromosome using primers listed below marR_BB_F TTCGAATTCGCGGCCGCTTCTAGATGAAAAGTACCAGCGATCT marR_BB_R GGCCGCTACTAGTATTATTACGGCAGGACTTTCTT
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: marR
sequence: https://synbiohub.org/public/igem/BBa_K864200_sequence/1

marR
type: SequenceAnnotation
persistent identity: annotation2197174
display id: annotation2197174
version: 1
top level: BBa_K864200
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K864200/annotation2197174/range2197174/1

https://synbiohub.org/public/igem/BBa_K864200/annotation2197174/range2197174/1
type: Range
persistent identity: range2197174
display id: range2197174
version: 1
direction: direction/forward
top level: BBa_K864200
start: 1
end: 438
orientation: inline

BBa_K772009
type: ComponentDefinition
persistent identity: BBa K772009
display id: BBa_K772009
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: GFP - LasR protein complex with flag tags
creator: Mustafa Elitok
created: 2012-09-25T11:00:00Z
modified: 2015-05-08T01:13:15Z
discontinued: false
dominant: false
group u list: _1024_
m user id: 0
owner id: 9573
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This part contains a green fluorescent protein (GFP) as a non-reactive chemical compound (anchor) in order to neutralize the active reactive fixed to it, an inducer regulatory protein (LasR is responsible to induce pLas promoter in the presence of 6HSLO3 which is a chemical compound sythesized by an enzyme that LasI gene codes.) and a TEV protease cleavage site between them to assemble these two proteins. BBa_772001 and BBa_K772002 are assembled to be used as the trigger of detection in the whole system which ends with the intracellular transfection (or activation) of TEV protease. After the activation, it is planned that TEV protease will cleave this anchor-inducer protein complex to release free the reporter. In conclusion, in this system, the inducer would activate a transcription pathway on the targeted inducible gene or the reporter would indicate that there is a change in the whole system. This part contains flag tags extra from BBa_K772006 in the GFP region; because it is planned to be used in the Co-IP/SDS-PAGE assay to be isolated.
mutable notes: Note that there is an RBS sequence just before the protein sequence in order to eliminate the need of addition of it.
mutable provenance: Cleavage site, LasR and GFP sequences are gathered from Registry.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Measurement
sequence annotation: TEV cleavage site
sequence annotation: LasR
sequence annotation: Double terminator
sequence annotation: RBS
sequence annotation: GFP
sequence annotation: Flag tag
sequence annotation: Anchor-inducer + flag
sequence: https://synbiohub.org/public/igem/BBa_K772009_sequence/1

TEV cleavage site
type: SequenceAnnotation
persistent identity: annotation2203920
display id: annotation2203920
version: 1
top level: BBa_K772009
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K772009/annotation2203920/range2203920/1

https://synbiohub.org/public/igem/BBa_K772009/annotation2203920/range2203920/1
type: Range
persistent identity: range2203920
display id: range2203920
version: 1
direction: direction/forward
top level: BBa_K772009
start: 768
end: 857
orientation: inline

LasR
type: SequenceAnnotation
persistent identity: annotation2203921
display id: annotation2203921
version: 1
top level: BBa_K772009
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K772009/annotation2203921/range2203921/1

https://synbiohub.org/public/igem/BBa_K772009/annotation2203921/range2203921/1
type: Range
persistent identity: range2203921
display id: range2203921
version: 1
direction: direction/forward
top level: BBa_K772009
start: 858
end: 1614
orientation: inline

Double terminator
type: SequenceAnnotation
persistent identity: annotation2203922
display id: annotation2203922
version: 1
top level: BBa_K772009
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K772009/annotation2203922/range2203922/1

https://synbiohub.org/public/igem/BBa_K772009/annotation2203922/range2203922/1
type: Range
persistent identity: range2203922
display id: range2203922
version: 1
direction: direction/forward
top level: BBa_K772009
start: 1615
end: 1745
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2203917
display id: annotation2203917
version: 1
top level: BBa_K772009
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K772009/annotation2203917/range2203917/1

https://synbiohub.org/public/igem/BBa_K772009/annotation2203917/range2203917/1
type: Range
persistent identity: range2203917
display id: range2203917
version: 1
direction: direction/forward
top level: BBa_K772009
start: 1
end: 20
orientation: inline

GFP
type: SequenceAnnotation
persistent identity: annotation2203919
display id: annotation2203919
version: 1
top level: BBa_K772009
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K772009/annotation2203919/range2203919/1

https://synbiohub.org/public/igem/BBa_K772009/annotation2203919/range2203919/1
type: Range
persistent identity: range2203919
display id: range2203919
version: 1
direction: direction/forward
top level: BBa_K772009
start: 47
end: 767
orientation: inline

Flag tag
type: SequenceAnnotation
persistent identity: annotation2203918
display id: annotation2203918
version: 1
top level: BBa_K772009
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K772009/annotation2203918/range2203918/1

https://synbiohub.org/public/igem/BBa_K772009/annotation2203918/range2203918/1
type: Range
persistent identity: range2203918
display id: range2203918
version: 1
direction: direction/forward
top level: BBa_K772009
start: 21
end: 46
orientation: inline

Anchor-inducer + flag
type: SequenceAnnotation
persistent identity: annotation2203923
display id: annotation2203923
version: 1
top level: BBa_K772009
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K772009/annotation2203923/range2203923/1

https://synbiohub.org/public/igem/BBa_K772009/annotation2203923/range2203923/1
type: Range
persistent identity: range2203923
display id: range2203923
version: 1
direction: direction/forward
top level: BBa_K772009
start: 21
end: 1614
orientation: inline

BBa_K1520502
type: ComponentDefinition
persistent identity: BBa K1520502
display id: BBa_K1520502
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Rbs-lacI(scarless)
creator: yue wu
created: 2014-09-21T11:00:00Z
modified: 2015-05-08T01:10:49Z
discontinued: false
dominant: false
group u list: _1902_
m user id: 0
owner id: 21318
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Rbs-lacI(scarless) It is an improvement of lacI by adding a rbs.
mutable notes: None
mutable provenance: It is an improvement of lacI by adding a rbs.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
sequence annotation: rbs
sequence annotation: lacI
sequence: https://synbiohub.org/public/igem/BBa_K1520502_sequence/1

rbs
type: SequenceAnnotation
persistent identity: annotation2384863
display id: annotation2384863
version: 1
top level: BBa_K1520502
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1520502/annotation2384863/range2384863/1

https://synbiohub.org/public/igem/BBa_K1520502/annotation2384863/range2384863/1
type: Range
persistent identity: range2384863
display id: range2384863
version: 1
direction: direction/forward
top level: BBa_K1520502
start: 1
end: 18
orientation: inline

lacI
type: SequenceAnnotation
persistent identity: annotation2384864
display id: annotation2384864
version: 1
top level: BBa_K1520502
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1520502/annotation2384864/range2384864/1

https://synbiohub.org/public/igem/BBa_K1520502/annotation2384864/range2384864/1
type: Range
persistent identity: range2384864
display id: range2384864
version: 1
direction: direction/forward
top level: BBa_K1520502
start: 19
end: 1171
orientation: inline

BBa_K1343001
type: ComponentDefinition
persistent identity: BBa K1343001
display id: BBa_K1343001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: P_ompC->NheI->RBS->luxI->double terminator
creator: Ronen Ben Jehuda
created: 2014-09-21T11:00:00Z
modified: 2015-05-08T01:09:59Z
discontinued: false
dominant: false
group u list: _1718_
m user id: 0
owner id: 20568
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part generates AHL (Source organism: V. fischeri). The promoter is P_ompC which induced by the Taz receptor which is cloned on a different plasmid as a different patr. Before the promoter there is the prefix site and after the promoter there is a restriction site of NheI, so the promoter can be replaced by a restriction enzyme reaction. In addition, after the NheI restriction site, there is an RBS and luxI which is coding to AHL. At the end of the part, there is double terminator and the Suffix site.
mutable notes: The part has been sequenced and has been checked with a detector strain which detects AHL and produce color. We know for sure that it produces AHL.
mutable provenance: P_ompC: BBa_R0082 RBS: BBa_B0030 luxI: BBa_C0061 but we removed the LVA tag (gctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctc) and added stop codon: "tag". Double terminator BBa_B0015
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: luxI
sequence annotation: Double terminator
sequence annotation: NehI
sequence annotation: spacer
sequence annotation: P_ompC
sequence: https://synbiohub.org/public/igem/BBa_K1343001_sequence/1

luxI
type: SequenceAnnotation
persistent identity: annotation2384921
display id: annotation2384921
version: 1
top level: BBa_K1343001
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1343001/annotation2384921/range2384921/1

https://synbiohub.org/public/igem/BBa_K1343001/annotation2384921/range2384921/1
type: Range
persistent identity: range2384921
display id: range2384921
version: 1
direction: direction/forward
top level: BBa_K1343001
start: 141
end: 722
orientation: inline

Double terminator
type: SequenceAnnotation
persistent identity: annotation2384922
display id: annotation2384922
version: 1
top level: BBa_K1343001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1343001/annotation2384922/range2384922/1

https://synbiohub.org/public/igem/BBa_K1343001/annotation2384922/range2384922/1
type: Range
persistent identity: range2384922
display id: range2384922
version: 1
direction: direction/forward
top level: BBa_K1343001
start: 723
end: 851
orientation: inline

NehI
type: SequenceAnnotation
persistent identity: annotation2384909
display id: annotation2384909
version: 1
top level: BBa_K1343001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1343001/annotation2384909/range2384909/1

https://synbiohub.org/public/igem/BBa_K1343001/annotation2384909/range2384909/1
type: Range
persistent identity: range2384909
display id: range2384909
version: 1
direction: direction/forward
top level: BBa_K1343001
start: 109
end: 114
orientation: inline

spacer
type: SequenceAnnotation
persistent identity: annotation2384920
display id: annotation2384920
version: 1
top level: BBa_K1343001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1343001/annotation2384920/range2384920/1

https://synbiohub.org/public/igem/BBa_K1343001/annotation2384920/range2384920/1
type: Range
persistent identity: range2384920
display id: range2384920
version: 1
direction: direction/forward
top level: BBa_K1343001
start: 115
end: 140
orientation: inline

P_ompC
type: SequenceAnnotation
persistent identity: annotation2384908
display id: annotation2384908
version: 1
top level: BBa_K1343001
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1343001/annotation2384908/range2384908/1

https://synbiohub.org/public/igem/BBa_K1343001/annotation2384908/range2384908/1
type: Range
persistent identity: range2384908
display id: range2384908
version: 1
direction: direction/forward
top level: BBa_K1343001
start: 1
end: 108
orientation: inline

lexA
type: ComponentDefinition
persistent identity: BBa K864201
display id: BBa_K864201
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LexA SOS response repressor
creator: Erik Lundin
created: 2012-09-24T11:00:00Z
modified: 2015-05-08T01:13:37Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1124_
m user id: 0
owner id: 9827
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: lexA is important in induction of recA
mutable notes: None
mutable provenance: Amplified from MG1655 (accession NC_000913) chromosome using primers listed below (annealing sequences are underlined) lexA_BB_F TTCGAATTCGCGGCCGCTTCTAGATGAAAGCGTTAACGGCCAG lexA_BB_R CGGCCGCTACTAGTATTATTACAGCCAGTCGCCGTT
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: lexA
sequence: https://synbiohub.org/public/igem/BBa_K864201_sequence/1

lexA
type: SequenceAnnotation
persistent identity: annotation2197113
display id: annotation2197113
version: 1
top level: lexA
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K864201/annotation2197113/range2197113/1

https://synbiohub.org/public/igem/BBa_K864201/annotation2197113/range2197113/1
type: Range
persistent identity: range2197113
display id: range2197113
version: 1
direction: direction/forward
top level: lexA
start: 1
end: 612
orientation: inline

BBa_K165050
type: ComponentDefinition
persistent identity: BBa K165050
display id: BBa_K165050
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Full Gli1 Repressor (tagged with mCherryx2)
creator: John Szymanski
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:10:56Z
discontinued: false
dominant: false
experience: experience/None
group u list: _267_
m user id: 0
owner id: 2512
owning group id: 58
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: Kozak+Sin3p RD+Gli1 DBD+mCherryx2+SV40NLS+tADH1
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K165004
component: BBa_K165002
component: BBa_K165003
component: BBa_K165018
component: BBa_K165007
sequence annotation: BBa_K165003
sequence annotation: BBa_K165018
sequence annotation: BBa_K165007
sequence annotation: BBa_K165004
sequence annotation: BBa_K165002
sequence: https://synbiohub.org/public/igem/BBa_K165050_sequence/1

BBa_K165004
type: Component
persistent identity: component1996711
display id: component1996711
version: 1
top level: BBa_K165050
access: public
definition: https://synbiohub.org/public/igem/BBa_K165004/1

BBa_K165002
type: Component
persistent identity: component1996708
display id: component1996708
version: 1
top level: BBa_K165050
access: public
definition: https://synbiohub.org/public/igem/BBa_K165002/1

BBa_K165003
type: Component
persistent identity: component1996709
display id: component1996709
version: 1
top level: BBa_K165050
access: public
definition: https://synbiohub.org/public/igem/BBa_K165003/1

BBa_K165018
type: Component
persistent identity: component1996713
display id: component1996713
version: 1
top level: BBa_K165050
access: public
definition: https://synbiohub.org/public/igem/BBa_K165018/1

BBa_K165004
type: Component
persistent identity: component1996712
display id: component1996712
version: 1
top level: BBa_K165050
access: public
definition: https://synbiohub.org/public/igem/BBa_K165004/1

BBa_K165007
type: Component
persistent identity: component1996710
display id: component1996710
version: 1
top level: BBa_K165050
access: public
definition: https://synbiohub.org/public/igem/BBa_K165007/1

BBa_K165003
type: SequenceAnnotation
persistent identity: annotation1996709
display id: annotation1996709
version: 1
top level: BBa_K165050
location: https://synbiohub.org/public/igem/BBa_K165050/annotation1996709/range1996709/1
component: BBa_K165003

https://synbiohub.org/public/igem/BBa_K165050/annotation1996709/range1996709/1
type: Range
persistent identity: range1996709
display id: range1996709
version: 1
direction: direction/reverse
top level: BBa_K165050
start: 27
end: 2837
orientation: inline

BBa_K165018
type: SequenceAnnotation
persistent identity: annotation1996713
display id: annotation1996713
version: 1
top level: BBa_K165050
location: https://synbiohub.org/public/igem/BBa_K165050/annotation1996713/range1996713/1
component: BBa_K165018

https://synbiohub.org/public/igem/BBa_K165050/annotation1996713/range1996713/1
type: Range
persistent identity: range1996713
display id: range1996713
version: 1
direction: direction/reverse
top level: BBa_K165050
start: 4865
end: 5117
orientation: inline

BBa_K165007
type: SequenceAnnotation
persistent identity: annotation1996710
display id: annotation1996710
version: 1
top level: BBa_K165050
location: https://synbiohub.org/public/igem/BBa_K165050/annotation1996710/range1996710/1
component: BBa_K165007

https://synbiohub.org/public/igem/BBa_K165050/annotation1996710/range1996710/1
type: Range
persistent identity: range1996710
display id: range1996710
version: 1
direction: direction/reverse
top level: BBa_K165050
start: 2846
end: 3370
orientation: inline

BBa_K165004
type: SequenceAnnotation
persistent identity: annotation1996712
display id: annotation1996712
version: 1
top level: BBa_K165050
location: https://synbiohub.org/public/igem/BBa_K165050/annotation1996712/range1996712/1
component: BBa_K165004

https://synbiohub.org/public/igem/BBa_K165050/annotation1996712/range1996712/1
type: Range
persistent identity: range1996712
display id: range1996712
version: 1
direction: direction/reverse
top level: BBa_K165050
start: 4122
end: 4856
orientation: inline

BBa_K165002
type: SequenceAnnotation
persistent identity: annotation1996708
display id: annotation1996708
version: 1
top level: BBa_K165050
location: https://synbiohub.org/public/igem/BBa_K165050/annotation1996708/range1996708/1
component: BBa_K165002

https://synbiohub.org/public/igem/BBa_K165050/annotation1996708/range1996708/1
type: Range
persistent identity: range1996708
display id: range1996708
version: 1
direction: direction/reverse
top level: BBa_K165050
start: 1
end: 18
orientation: inline

BBa_K165004
type: SequenceAnnotation
persistent identity: annotation1996711
display id: annotation1996711
version: 1
top level: BBa_K165050
location: https://synbiohub.org/public/igem/BBa_K165050/annotation1996711/range1996711/1
component: BBa_K165004

https://synbiohub.org/public/igem/BBa_K165050/annotation1996711/range1996711/1
type: Range
persistent identity: range1996711
display id: range1996711
version: 1
direction: direction/reverse
top level: BBa_K165050
start: 3379
end: 4113
orientation: inline

BBa_J06650
type: ComponentDefinition
persistent identity: BBa J06650
display id: BBa_J06650
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Test of BBa_R0051
creator: ytwang
created: 2005-06-13T11:00:00Z
modified: 2015-08-31T04:08:18Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _20_
m user id: 0
owner id: 340
owning group id: 20
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0034
component: BBa_B0010
component: BBa_E0030
component: BBa_R0051
component: BBa_B0012
sequence annotation: BBa_B0034
sequence annotation: BBa_B0012
sequence annotation: BBa_R0051
sequence annotation: BBa_B0010
sequence annotation: BBa_E0030
sequence: https://synbiohub.org/public/igem/BBa_J06650_sequence/1

BBa_B0034
type: Component
persistent identity: component1533890
display id: component1533890
version: 1
top level: BBa_J06650
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0010
type: Component
persistent identity: component1533897
display id: component1533897
version: 1
top level: BBa_J06650
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_E0030
type: Component
persistent identity: component1533892
display id: component1533892
version: 1
top level: BBa_J06650
access: public
definition: https://synbiohub.org/public/igem/BBa_E0030/1

BBa_R0051
type: Component
persistent identity: component1533882
display id: component1533882
version: 1
top level: BBa_J06650
access: public
definition: https://synbiohub.org/public/igem/BBa_R0051/1

BBa_B0012
type: Component
persistent identity: component1533907
display id: component1533907
version: 1
top level: BBa_J06650
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1533890
display id: annotation1533890
version: 1
top level: BBa_J06650
location: https://synbiohub.org/public/igem/BBa_J06650/annotation1533890/range1533890/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_J06650/annotation1533890/range1533890/1
type: Range
persistent identity: range1533890
display id: range1533890
version: 1
direction: direction/forward
top level: BBa_J06650
start: 58
end: 69
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1533907
display id: annotation1533907
version: 1
top level: BBa_J06650
location: https://synbiohub.org/public/igem/BBa_J06650/annotation1533907/range1533907/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_J06650/annotation1533907/range1533907/1
type: Range
persistent identity: range1533907
display id: range1533907
version: 1
direction: direction/forward
top level: BBa_J06650
start: 895
end: 935
orientation: inline

BBa_R0051
type: SequenceAnnotation
persistent identity: annotation1533882
display id: annotation1533882
version: 1
top level: BBa_J06650
location: https://synbiohub.org/public/igem/BBa_J06650/annotation1533882/range1533882/1
component: BBa_R0051

https://synbiohub.org/public/igem/BBa_J06650/annotation1533882/range1533882/1
type: Range
persistent identity: range1533882
display id: range1533882
version: 1
direction: direction/forward
top level: BBa_J06650
start: 1
end: 49
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1533897
display id: annotation1533897
version: 1
top level: BBa_J06650
location: https://synbiohub.org/public/igem/BBa_J06650/annotation1533897/range1533897/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_J06650/annotation1533897/range1533897/1
type: Range
persistent identity: range1533897
display id: range1533897
version: 1
direction: direction/forward
top level: BBa_J06650
start: 807
end: 886
orientation: inline

BBa_E0030
type: SequenceAnnotation
persistent identity: annotation1533892
display id: annotation1533892
version: 1
top level: BBa_J06650
location: https://synbiohub.org/public/igem/BBa_J06650/annotation1533892/range1533892/1
component: BBa_E0030

https://synbiohub.org/public/igem/BBa_J06650/annotation1533892/range1533892/1
type: Range
persistent identity: range1533892
display id: range1533892
version: 1
direction: direction/forward
top level: BBa_J06650
start: 76
end: 798
orientation: inline

BBa_K091203
type: ComponentDefinition
persistent identity: BBa K091203
display id: BBa_K091203
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pBAD-RBS-LuxR-RBS-LacI(I12)-TT
creator: Todd Eckdahl
created: 2008-09-03T11:00:00Z
modified: 2015-05-08T01:08:39Z
discontinued: false
dominant: false
experience: experience/None
group u list: _191_
m user id: 0
owner id: 606
owning group id: 61
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This expression casette constitutively produces the LuxR regulatory protein that can respond to the quorum sensing molecule 3OC6. It also produces a LacI repressor with a mutation at position 12 that results in an isoleucine. The mutant repressor has been shown to have an increased affinity for its operator.
mutable notes: None.
mutable provenance: Intermediate parts.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_K091166
component: BBa_S04058
sequence annotation: BBa_S04058
sequence annotation: BBa_K091166
sequence: https://synbiohub.org/public/igem/BBa_K091203_sequence/1

BBa_K091166
type: Component
persistent identity: component2266896
display id: component2266896
version: 1
top level: BBa_K091203
access: public
definition: https://synbiohub.org/public/igem/BBa_K091166/1

BBa_S04058
type: Component
persistent identity: component2266907
display id: component2266907
version: 1
top level: BBa_K091203
access: public
definition: https://synbiohub.org/public/igem/BBa_S04058/1

BBa_S04058
type: SequenceAnnotation
persistent identity: annotation2266907
display id: annotation2266907
version: 1
top level: BBa_K091203
location: https://synbiohub.org/public/igem/BBa_K091203/annotation2266907/range2266907/1
component: BBa_S04058

https://synbiohub.org/public/igem/BBa_K091203/annotation2266907/range2266907/1
type: Range
persistent identity: range2266907
display id: range2266907
version: 1
direction: direction/reverse
top level: BBa_K091203
start: 946
end: 2183
orientation: inline

BBa_K091166
type: SequenceAnnotation
persistent identity: annotation2266896
display id: annotation2266896
version: 1
top level: BBa_K091203
location: https://synbiohub.org/public/igem/BBa_K091203/annotation2266896/range2266896/1
component: BBa_K091166

https://synbiohub.org/public/igem/BBa_K091203/annotation2266896/range2266896/1
type: Range
persistent identity: range2266896
display id: range2266896
version: 1
direction: direction/reverse
top level: BBa_K091203
start: 1
end: 937
orientation: inline

BBa_K1486043
type: ComponentDefinition
persistent identity: BBa K1486043
display id: BBa_K1486043
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Leucine Zipper + split rLuc
creator: EPFL iGem team 2014
created: 2014-10-01T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: To edit. Pense ?? rajouter les linkers, je ne sais pas s'ils sont de igem ou s'il faut ajouter la s??quence ?? la registry. Check pour tous tes biobricks et dis-moi, please.
mutable notes: To edit
mutable provenance: To edit
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0034
component: BBa_K1486042
component: BBa_K1486019
component: BBa_K1486020
component: BBa_I0500
sequence annotation: BBa_K1486020
sequence annotation: BBa_K1486042
sequence annotation: BBa_K1486019
sequence annotation: BBa_B0034
sequence annotation: BBa_I0500
sequence: https://synbiohub.org/public/igem/BBa_K1486043_sequence/1

BBa_B0034
type: Component
persistent identity: component2392172
display id: component2392172
version: 1
top level: BBa_K1486043
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0034
type: Component
persistent identity: component2392176
display id: component2392176
version: 1
top level: BBa_K1486043
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K1486042
type: Component
persistent identity: component2392177
display id: component2392177
version: 1
top level: BBa_K1486043
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486042/1

BBa_K1486042
type: Component
persistent identity: component2392173
display id: component2392173
version: 1
top level: BBa_K1486043
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486042/1

BBa_K1486019
type: Component
persistent identity: component2392174
display id: component2392174
version: 1
top level: BBa_K1486043
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486019/1

BBa_K1486020
type: Component
persistent identity: component2392178
display id: component2392178
version: 1
top level: BBa_K1486043
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486020/1

BBa_I0500
type: Component
persistent identity: component2392170
display id: component2392170
version: 1
top level: BBa_K1486043
access: public
definition: https://synbiohub.org/public/igem/BBa_I0500/1

BBa_K1486020
type: SequenceAnnotation
persistent identity: annotation2392178
display id: annotation2392178
version: 1
top level: BBa_K1486043
location: https://synbiohub.org/public/igem/BBa_K1486043/annotation2392178/range2392178/1
component: BBa_K1486020

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type: Range
persistent identity: range2392178
display id: range2392178
version: 1
direction: direction/reverse
top level: BBa_K1486043
start: 1865
end: 2470
orientation: inline

BBa_K1486042
type: SequenceAnnotation
persistent identity: annotation2392173
display id: annotation2392173
version: 1
top level: BBa_K1486043
location: https://synbiohub.org/public/igem/BBa_K1486043/annotation2392173/range2392173/1
component: BBa_K1486042

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type: Range
persistent identity: range2392173
display id: range2392173
version: 1
direction: direction/reverse
top level: BBa_K1486043
start: 1223
end: 1372
orientation: inline

BBa_K1486019
type: SequenceAnnotation
persistent identity: annotation2392174
display id: annotation2392174
version: 1
top level: BBa_K1486043
location: https://synbiohub.org/public/igem/BBa_K1486043/annotation2392174/range2392174/1
component: BBa_K1486019

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type: Range
persistent identity: range2392174
display id: range2392174
version: 1
direction: direction/reverse
top level: BBa_K1486043
start: 1373
end: 1702
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2392172
display id: annotation2392172
version: 1
top level: BBa_K1486043
location: https://synbiohub.org/public/igem/BBa_K1486043/annotation2392172/range2392172/1
component: BBa_B0034

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type: Range
persistent identity: range2392172
display id: range2392172
version: 1
direction: direction/reverse
top level: BBa_K1486043
start: 1211
end: 1222
orientation: inline

BBa_I0500
type: SequenceAnnotation
persistent identity: annotation2392170
display id: annotation2392170
version: 1
top level: BBa_K1486043
location: https://synbiohub.org/public/igem/BBa_K1486043/annotation2392170/range2392170/1
component: BBa_I0500

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type: Range
persistent identity: range2392170
display id: range2392170
version: 1
direction: direction/reverse
top level: BBa_K1486043
start: 1
end: 1210
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2392176
display id: annotation2392176
version: 1
top level: BBa_K1486043
location: https://synbiohub.org/public/igem/BBa_K1486043/annotation2392176/range2392176/1
component: BBa_B0034

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type: Range
persistent identity: range2392176
display id: range2392176
version: 1
direction: direction/reverse
top level: BBa_K1486043
start: 1703
end: 1714
orientation: inline

BBa_K1486042
type: SequenceAnnotation
persistent identity: annotation2392177
display id: annotation2392177
version: 1
top level: BBa_K1486043
location: https://synbiohub.org/public/igem/BBa_K1486043/annotation2392177/range2392177/1
component: BBa_K1486042

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type: Range
persistent identity: range2392177
display id: range2392177
version: 1
direction: direction/reverse
top level: BBa_K1486043
start: 1715
end: 1864
orientation: inline

BBa_K1031018
type: ComponentDefinition
persistent identity: BBa K1031018
display id: BBa_K1031018
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Psal-B0034-RiboJ-φR73δ
creator: He Shuaixin
created: 2013-10-21T11:00:00Z
modified: 2015-05-08T01:08:47Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1338_
m user id: 0
owner id: 15843
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Psal-φR73Δ
mutable notes: molecular cloning
mutable provenance: φR73
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: phiR73delta
sequence annotation: Psal
sequence: https://synbiohub.org/public/igem/BBa_K1031018_sequence/1

phiR73delta
type: SequenceAnnotation
persistent identity: annotation2370290
display id: annotation2370290
version: 1
top level: BBa_K1031018
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1031018/annotation2370290/range2370290/1

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type: Range
persistent identity: range2370290
display id: range2370290
version: 1
direction: direction/forward
top level: BBa_K1031018
start: 235
end: 480
orientation: inline

Psal
type: SequenceAnnotation
persistent identity: annotation2370289
display id: annotation2370289
version: 1
top level: BBa_K1031018
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1031018/annotation2370289/range2370289/1

https://synbiohub.org/public/igem/BBa_K1031018/annotation2370289/range2370289/1
type: Range
persistent identity: range2370289
display id: range2370289
version: 1
direction: direction/forward
top level: BBa_K1031018
start: 1
end: 228
orientation: inline

BBa_K1031016
type: ComponentDefinition
persistent identity: BBa K1031016
display id: BBa_K1031016
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: NahR-Psal-B0034-RiboJ-CI
creator: He Shuaixin
created: 2013-09-08T11:00:00Z
modified: 2015-05-08T01:08:47Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1338_
m user id: 0
owner id: 15843
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: NahR Psel Ribo_J CI B0034
mutable notes: Pseudomonas putida, phiR73 phage, E.coli
mutable provenance: lab made
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: cI
sequence annotation: NahR
sequence annotation: promoter
sequence annotation: B0034
sequence: https://synbiohub.org/public/igem/BBa_K1031016_sequence/1

cI
type: SequenceAnnotation
persistent identity: annotation2354032
display id: annotation2354032
version: 1
top level: BBa_K1031016
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1031016/annotation2354032/range2354032/1

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type: Range
persistent identity: range2354032
display id: range2354032
version: 1
direction: direction/forward
top level: BBa_K1031016
start: 1169
end: 1885
orientation: inline

NahR
type: SequenceAnnotation
persistent identity: annotation2354029
display id: annotation2354029
version: 1
top level: BBa_K1031016
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1031016/annotation2354029/range2354029/1

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type: Range
persistent identity: range2354029
display id: range2354029
version: 1
direction: direction/reverse
top level: BBa_K1031016
start: 1
end: 903
orientation: inline

promoter
type: SequenceAnnotation
persistent identity: annotation2354030
display id: annotation2354030
version: 1
top level: BBa_K1031016
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1031016/annotation2354030/range2354030/1

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type: Range
persistent identity: range2354030
display id: range2354030
version: 1
direction: direction/forward
top level: BBa_K1031016
start: 904
end: 1050
orientation: inline

B0034
type: SequenceAnnotation
persistent identity: annotation2354031
display id: annotation2354031
version: 1
top level: BBa_K1031016
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1031016/annotation2354031/range2354031/1

https://synbiohub.org/public/igem/BBa_K1031016/annotation2354031/range2354031/1
type: Range
persistent identity: range2354031
display id: range2354031
version: 1
direction: direction/forward
top level: BBa_K1031016
start: 1150
end: 1165
orientation: inline

penI
type: ComponentDefinition
persistent identity: BBa C0074
display id: BBa_C0074
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: penI repressor from Bacillus licheniformis (+LVA)
creator: crackdots
created: 2004-01-27T12:00:00Z
modified: 2015-08-31T04:07:23Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: -- No description --
mutable notes: Since penI is being ported from Bacillus Licheniformis, the sequence was changed within the coding region to reflect codon usage in E.Coli K12. Codons were mapped to the codon of the same usage rank in E.Coli.
mutable provenance: bacillus licheniformis
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: penI
sequence annotation: Help:Barcodes
sequence annotation: LVA
sequence: https://synbiohub.org/public/igem/BBa_C0074_sequence/1

penI
type: SequenceAnnotation
persistent identity: annotation302638
display id: annotation302638
version: 1
top level: penI
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_C0074/annotation302638/range302638/1

https://synbiohub.org/public/igem/BBa_C0074/annotation302638/range302638/1
type: Range
persistent identity: range302638
display id: range302638
version: 1
direction: direction/forward
top level: penI
start: 1
end: 387
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2214009
display id: annotation2214009
version: 1
top level: penI
role: SO:0000807
role: feature/barcode
location: https://synbiohub.org/public/igem/BBa_C0074/annotation2214009/range2214009/1

https://synbiohub.org/public/igem/BBa_C0074/annotation2214009/range2214009/1
type: Range
persistent identity: range2214009
display id: range2214009
version: 1
direction: direction/forward
top level: penI
start: 424
end: 448
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation306563
display id: annotation306563
version: 1
top level: penI
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_C0074/annotation306563/range306563/1

https://synbiohub.org/public/igem/BBa_C0074/annotation306563/range306563/1
type: Range
persistent identity: range306563
display id: range306563
version: 1
direction: direction/forward
top level: penI
start: 385
end: 417
orientation: inline

BBa_K1520024
type: ComponentDefinition
persistent identity: BBa K1520024
display id: BBa_K1520024
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Pcons2-rbs-lacI-Ter-Plac-rbs-luxI-Ter-Pcons2-rbs-luxR-Ter-Prlux-rbs-rfp-rbs-luxI-rbs-luxR-Ter
creator: yue wu
created: 2014-09-21T11:00:00Z
modified: 2015-05-08T01:10:49Z
discontinued: false
dominant: false
group u list: _1902_
m user id: 0
owner id: 21318
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Plasmid in positive feedback verification: Pcons2-rbs-lacI-Ter-Plac-rbs-luxI-Ter-Pcons2-rbs-luxR-Ter-Prlux-rbs-rfp-rbs-luxI-rbs-luxR-Ter It is a positive feedback plasmid using luxI and luxR as key to feedback. Introducing IPTG will produce rfp, luxI and luxR, and luxI together with luxR will work back to enhance production of rfp.
mutable notes: None
mutable provenance: Pcons2, lacI, Plac, luxI, luxR, Prlux, rfp, Ter
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1520013
component: BBa_K1520020
sequence annotation: BBa_K1520013
sequence annotation: BBa_K1520020
sequence: https://synbiohub.org/public/igem/BBa_K1520024_sequence/1

BBa_K1520013
type: Component
persistent identity: component2385264
display id: component2385264
version: 1
top level: BBa_K1520024
access: public
definition: BBa_K1520013

BBa_K1520020
type: Component
persistent identity: component2385250
display id: component2385250
version: 1
top level: BBa_K1520024
access: public
definition: BBa_K1520020

BBa_K1520013
type: SequenceAnnotation
persistent identity: annotation2385264
display id: annotation2385264
version: 1
top level: BBa_K1520024
location: https://synbiohub.org/public/igem/BBa_K1520024/annotation2385264/range2385264/1
component: BBa_K1520013

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type: Range
persistent identity: range2385264
display id: range2385264
version: 1
direction: direction/reverse
top level: BBa_K1520024
start: 3361
end: 5768
orientation: inline

BBa_K1520020
type: SequenceAnnotation
persistent identity: annotation2385250
display id: annotation2385250
version: 1
top level: BBa_K1520024
location: https://synbiohub.org/public/igem/BBa_K1520024/annotation2385250/range2385250/1
component: BBa_K1520020

https://synbiohub.org/public/igem/BBa_K1520024/annotation2385250/range2385250/1
type: Range
persistent identity: range2385250
display id: range2385250
version: 1
direction: direction/reverse
top level: BBa_K1520024
start: 1
end: 3352
orientation: inline

BBa_K165088
type: ComponentDefinition
persistent identity: BBa K165088
display id: BBa_K165088
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Zif268-HIV bs + TEF2 + Untagged LexA activator on pRS306
creator: John Szymanski
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:10:57Z
discontinued: false
dominant: false
group u list: _267_
m user id: 0
owner id: 2512
owning group id: 58
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: -
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_K165037
component: BBa_K165011
component: BBa_K165020
sequence annotation: BBa_K165037
sequence annotation: BBa_K165020
sequence annotation: BBa_K165011
sequence: https://synbiohub.org/public/igem/BBa_K165088_sequence/1

BBa_K165037
type: Component
persistent identity: component1997204
display id: component1997204
version: 1
top level: BBa_K165088
access: public
definition: https://synbiohub.org/public/igem/BBa_K165037/1

BBa_K165011
type: Component
persistent identity: component1997203
display id: component1997203
version: 1
top level: BBa_K165088
access: public
definition: https://synbiohub.org/public/igem/BBa_K165011/1

BBa_K165020
type: Component
persistent identity: component1997205
display id: component1997205
version: 1
top level: BBa_K165088
access: public
definition: lexA

BBa_K165037
type: SequenceAnnotation
persistent identity: annotation1997204
display id: annotation1997204
version: 1
top level: BBa_K165088
location: https://synbiohub.org/public/igem/BBa_K165088/annotation1997204/range1997204/1
component: BBa_K165037

https://synbiohub.org/public/igem/BBa_K165088/annotation1997204/range1997204/1
type: Range
persistent identity: range1997204
display id: range1997204
version: 1
direction: direction/reverse
top level: BBa_K165088
start: 55
end: 457
orientation: inline

BBa_K165020
type: SequenceAnnotation
persistent identity: annotation1997205
display id: annotation1997205
version: 1
top level: BBa_K165088
location: https://synbiohub.org/public/igem/BBa_K165088/annotation1997205/range1997205/1
component: BBa_K165020

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type: Range
persistent identity: range1997205
display id: range1997205
version: 1
direction: direction/reverse
top level: BBa_K165088
start: 466
end: 1137
orientation: inline

BBa_K165011
type: SequenceAnnotation
persistent identity: annotation1997203
display id: annotation1997203
version: 1
top level: BBa_K165088
location: https://synbiohub.org/public/igem/BBa_K165088/annotation1997203/range1997203/1
component: BBa_K165011

https://synbiohub.org/public/igem/BBa_K165088/annotation1997203/range1997203/1
type: Range
persistent identity: range1997203
display id: range1997203
version: 1
direction: direction/reverse
top level: BBa_K165088
start: 1
end: 46
orientation: inline

BBa_K1520003
type: ComponentDefinition
persistent identity: BBa K1520003
display id: BBa_K1520003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Pcons2-rbs-luxR-Ter
creator: yue wu
created: 2014-09-21T11:00:00Z
modified: 2015-05-08T01:10:48Z
discontinued: false
dominant: false
group u list: _1902_
m user id: 0
owner id: 21318
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Pcons2-rbs-luxR-Ter. It achieves a constant expression of luxR, the AHL combining protein Pcons2 is BBa_J23100
mutable notes: NONE
mutable provenance: Pcons2, luxR, Ter
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K1520501
component: BBa_K1520029
component: BBa_J23100
sequence annotation: BBa_K1520501
sequence annotation: BBa_J23100
sequence annotation: BBa_K1520029
sequence: https://synbiohub.org/public/igem/BBa_K1520003_sequence/1

BBa_K1520501
type: Component
persistent identity: component2384887
display id: component2384887
version: 1
top level: BBa_K1520003
access: public
definition: BBa_K1520501

BBa_K1520029
type: Component
persistent identity: component2384888
display id: component2384888
version: 1
top level: BBa_K1520003
access: public
definition: https://synbiohub.org/public/igem/BBa_K1520029/1

BBa_J23100
type: Component
persistent identity: component2384884
display id: component2384884
version: 1
top level: BBa_K1520003
access: public
definition: https://synbiohub.org/public/igem/BBa_J23100/1

BBa_K1520501
type: SequenceAnnotation
persistent identity: annotation2384887
display id: annotation2384887
version: 1
top level: BBa_K1520003
location: https://synbiohub.org/public/igem/BBa_K1520003/annotation2384887/range2384887/1
component: BBa_K1520501

https://synbiohub.org/public/igem/BBa_K1520003/annotation2384887/range2384887/1
type: Range
persistent identity: range2384887
display id: range2384887
version: 1
direction: direction/reverse
top level: BBa_K1520003
start: 44
end: 842
orientation: inline

BBa_J23100
type: SequenceAnnotation
persistent identity: annotation2384884
display id: annotation2384884
version: 1
top level: BBa_K1520003
location: https://synbiohub.org/public/igem/BBa_K1520003/annotation2384884/range2384884/1
component: BBa_J23100

https://synbiohub.org/public/igem/BBa_K1520003/annotation2384884/range2384884/1
type: Range
persistent identity: range2384884
display id: range2384884
version: 1
direction: direction/reverse
top level: BBa_K1520003
start: 1
end: 35
orientation: inline

BBa_K1520029
type: SequenceAnnotation
persistent identity: annotation2384888
display id: annotation2384888
version: 1
top level: BBa_K1520003
location: https://synbiohub.org/public/igem/BBa_K1520003/annotation2384888/range2384888/1
component: BBa_K1520029

https://synbiohub.org/public/igem/BBa_K1520003/annotation2384888/range2384888/1
type: Range
persistent identity: range2384888
display id: range2384888
version: 1
direction: direction/reverse
top level: BBa_K1520003
start: 851
end: 979
orientation: inline

lacI ts
type: ComponentDefinition
persistent identity: BBa J06500
display id: BBa_J06500
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LacI repressor (temperature-sensitive mut 241) (+LVA)
creator: ytwang
created: 2005-06-13T11:00:00Z
modified: 2015-08-31T04:08:18Z
discontinued: false
dominant: false
experience: experience/None
group u list: _20_
m user id: 0
owner id: 340
owning group id: 20
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Mutated from BBa_C0012 Repressor, LacI (LVA+) to be temperature sensitive. Mutation from Yabuta, 1995: Ala241->Thr (G(730)CG->ACG)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: LacI(ts)-LVA
sequence annotation: TS mutation (G->A)
sequence annotation: LVA
sequence annotation: barcode from LacI (BBa_C0012)
sequence: https://synbiohub.org/public/igem/BBa_J06500_sequence/1

LacI(ts)-LVA
type: SequenceAnnotation
persistent identity: annotation1518213
display id: annotation1518213
version: 1
top level: lacI ts
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J06500/annotation1518213/range1518213/1

https://synbiohub.org/public/igem/BBa_J06500/annotation1518213/range1518213/1
type: Range
persistent identity: range1518213
display id: range1518213
version: 1
direction: direction/forward
top level: lacI ts
start: 1
end: 1128
orientation: inline

TS mutation (G->A)
type: SequenceAnnotation
persistent identity: annotation1518230
display id: annotation1518230
version: 1
top level: lacI ts
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_J06500/annotation1518230/range1518230/1

https://synbiohub.org/public/igem/BBa_J06500/annotation1518230/range1518230/1
type: Range
persistent identity: range1518230
display id: range1518230
version: 1
direction: direction/forward
top level: lacI ts
start: 730
end: 730
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation1518220
display id: annotation1518220
version: 1
top level: lacI ts
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_J06500/annotation1518220/range1518220/1

https://synbiohub.org/public/igem/BBa_J06500/annotation1518220/range1518220/1
type: Range
persistent identity: range1518220
display id: range1518220
version: 1
direction: direction/forward
top level: lacI ts
start: 1090
end: 1128
orientation: inline

barcode from LacI (BBa_C0012)
type: SequenceAnnotation
persistent identity: annotation1518229
display id: annotation1518229
version: 1
top level: lacI ts
role: SO:0000807
role: feature/barcode
location: https://synbiohub.org/public/igem/BBa_J06500/annotation1518229/range1518229/1

https://synbiohub.org/public/igem/BBa_J06500/annotation1518229/range1518229/1
type: Range
persistent identity: range1518229
display id: range1518229
version: 1
direction: direction/forward
top level: lacI ts
start: 1129
end: 1153
orientation: inline

BBa_K545008
type: ComponentDefinition
persistent identity: BBa K545008
display id: BBa_K545008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: rsmY iRNA
creator: Grenoble 2011
created: 2011-09-19T11:00:00Z
modified: 2015-05-08T01:12:39Z
discontinued: false
dominant: true
experience: experience/None
group u list: _712_
m user id: 0
owner id: 8648
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: RsmY codes for a iRNA which binds and inhibits the rsmA repressor.
mutable notes: This part was build following the standard construction protocol.
mutable provenance: Pseudomonas aeruginosa
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Other
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_K545008_sequence/1

BBa_K1486011
type: ComponentDefinition
persistent identity: BBa K1486011
display id: BBa_K1486011
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CxpR & Split IFP1.4 [Cterm + Nterm]
creator: iGem EPFL 2014
created: 2014-08-26T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part has two fused proteins downstream of an arabinose-inducible promoter: CpxR-IFP[1] and CpxR-IFP[2]. This construct was made in all orientations: - CpxR-IFP[1] + CpxR-IFP[2] (BBa_K1286008) - IFP[1]-CpxR + CpxR-IFP[2] (BBa_K1486010) - CpxR-IFP[1] + IFP[2]-CpxR (BBa_K1486011)
mutable notes: All orientations were made, so all dimerizations could be tested (see main page for biobrick numbers). Flexible linkers were used for each of the fused proteins, and an RBS was put before each one of them.
mutable provenance: From Michnick Article. To Edit
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_I0500
component: BBa_K1486000
component: BBa_K1486007
component: BBa_K1486004
component: BBa_K1486006
component: BBa_B0034
sequence annotation: BBa_I0500
sequence annotation: BBa_K1486006
sequence annotation: BBa_K1486000
sequence annotation: BBa_K1486007
sequence annotation: BBa_K1486004
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K1486011_sequence/1

BBa_I0500
type: Component
persistent identity: component2382235
display id: component2382235
version: 1
top level: BBa_K1486011
access: public
definition: https://synbiohub.org/public/igem/BBa_I0500/1

BBa_K1486000
type: Component
persistent identity: component2382254
display id: component2382254
version: 1
top level: BBa_K1486011
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486000/1

BBa_K1486007
type: Component
persistent identity: component2382249
display id: component2382249
version: 1
top level: BBa_K1486011
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486007/1

BBa_K1486004
type: Component
persistent identity: component2382250
display id: component2382250
version: 1
top level: BBa_K1486011
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486004/1

BBa_K1486006
type: Component
persistent identity: component2382243
display id: component2382243
version: 1
top level: BBa_K1486011
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486006/1

BBa_B0034
type: Component
persistent identity: component2382245
display id: component2382245
version: 1
top level: BBa_K1486011
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K1486004
type: Component
persistent identity: component2382242
display id: component2382242
version: 1
top level: BBa_K1486011
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486004/1

BBa_B0034
type: Component
persistent identity: component2382237
display id: component2382237
version: 1
top level: BBa_K1486011
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K1486000
type: Component
persistent identity: component2382241
display id: component2382241
version: 1
top level: BBa_K1486011
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486000/1

BBa_I0500
type: SequenceAnnotation
persistent identity: annotation2382235
display id: annotation2382235
version: 1
top level: BBa_K1486011
location: https://synbiohub.org/public/igem/BBa_K1486011/annotation2382235/range2382235/1
component: BBa_I0500

https://synbiohub.org/public/igem/BBa_K1486011/annotation2382235/range2382235/1
type: Range
persistent identity: range2382235
display id: range2382235
version: 1
direction: direction/reverse
top level: BBa_K1486011
start: 1
end: 1210
orientation: inline

BBa_K1486006
type: SequenceAnnotation
persistent identity: annotation2382243
display id: annotation2382243
version: 1
top level: BBa_K1486011
location: https://synbiohub.org/public/igem/BBa_K1486011/annotation2382243/range2382243/1
component: BBa_K1486006

https://synbiohub.org/public/igem/BBa_K1486011/annotation2382243/range2382243/1
type: Range
persistent identity: range2382243
display id: range2382243
version: 1
direction: direction/reverse
top level: BBa_K1486011
start: 1955
end: 2536
orientation: inline

BBa_K1486000
type: SequenceAnnotation
persistent identity: annotation2382241
display id: annotation2382241
version: 1
top level: BBa_K1486011
location: https://synbiohub.org/public/igem/BBa_K1486011/annotation2382241/range2382241/1
component: BBa_K1486000

https://synbiohub.org/public/igem/BBa_K1486011/annotation2382241/range2382241/1
type: Range
persistent identity: range2382241
display id: range2382241
version: 1
direction: direction/reverse
top level: BBa_K1486011
start: 1223
end: 1924
orientation: inline

BBa_K1486007
type: SequenceAnnotation
persistent identity: annotation2382249
display id: annotation2382249
version: 1
top level: BBa_K1486011
location: https://synbiohub.org/public/igem/BBa_K1486011/annotation2382249/range2382249/1
component: BBa_K1486007

https://synbiohub.org/public/igem/BBa_K1486011/annotation2382249/range2382249/1
type: Range
persistent identity: range2382249
display id: range2382249
version: 1
direction: direction/reverse
top level: BBa_K1486011
start: 2549
end: 3127
orientation: inline

BBa_K1486004
type: SequenceAnnotation
persistent identity: annotation2382250
display id: annotation2382250
version: 1
top level: BBa_K1486011
location: https://synbiohub.org/public/igem/BBa_K1486011/annotation2382250/range2382250/1
component: BBa_K1486004

https://synbiohub.org/public/igem/BBa_K1486011/annotation2382250/range2382250/1
type: Range
persistent identity: range2382250
display id: range2382250
version: 1
direction: direction/reverse
top level: BBa_K1486011
start: 3128
end: 3157
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2382237
display id: annotation2382237
version: 1
top level: BBa_K1486011
location: https://synbiohub.org/public/igem/BBa_K1486011/annotation2382237/range2382237/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1486011/annotation2382237/range2382237/1
type: Range
persistent identity: range2382237
display id: range2382237
version: 1
direction: direction/reverse
top level: BBa_K1486011
start: 1211
end: 1222
orientation: inline

BBa_K1486004
type: SequenceAnnotation
persistent identity: annotation2382242
display id: annotation2382242
version: 1
top level: BBa_K1486011
location: https://synbiohub.org/public/igem/BBa_K1486011/annotation2382242/range2382242/1
component: BBa_K1486004

https://synbiohub.org/public/igem/BBa_K1486011/annotation2382242/range2382242/1
type: Range
persistent identity: range2382242
display id: range2382242
version: 1
direction: direction/reverse
top level: BBa_K1486011
start: 1925
end: 1954
orientation: inline

BBa_K1486000
type: SequenceAnnotation
persistent identity: annotation2382254
display id: annotation2382254
version: 1
top level: BBa_K1486011
location: https://synbiohub.org/public/igem/BBa_K1486011/annotation2382254/range2382254/1
component: BBa_K1486000

https://synbiohub.org/public/igem/BBa_K1486011/annotation2382254/range2382254/1
type: Range
persistent identity: range2382254
display id: range2382254
version: 1
direction: direction/reverse
top level: BBa_K1486011
start: 3158
end: 3859
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2382245
display id: annotation2382245
version: 1
top level: BBa_K1486011
location: https://synbiohub.org/public/igem/BBa_K1486011/annotation2382245/range2382245/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1486011/annotation2382245/range2382245/1
type: Range
persistent identity: range2382245
display id: range2382245
version: 1
direction: direction/reverse
top level: BBa_K1486011
start: 2537
end: 2548
orientation: inline

BBa_P0153
type: ComponentDefinition
persistent identity: BBa P0153
display id: BBa_P0153
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PoPS -> cII (p22) [S0167]
creator: Randy Rettberg
created: 2004-04-24T11:00:00Z
modified: 2015-05-08T01:14:10Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Protein generator converting TIPS to the protein cII from p22. Used as the input section for Quad Part Inverters Q01530.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0031
component: BBa_B0010
component: BBa_B0012
component: BBa_C0053
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_C0053
sequence annotation: BBa_B0031
sequence: https://synbiohub.org/public/igem/BBa_P0153_sequence/1

BBa_B0031
type: Component
persistent identity: component944568
display id: component944568
version: 1
top level: BBa_P0153
access: public
definition: https://synbiohub.org/public/igem/BBa_B0031/1

BBa_B0010
type: Component
persistent identity: component944588
display id: component944588
version: 1
top level: BBa_P0153
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0012
type: Component
persistent identity: component944598
display id: component944598
version: 1
top level: BBa_P0153
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_C0053
type: Component
persistent identity: component944580
display id: component944580
version: 1
top level: BBa_P0153
access: public
definition: c2 P22

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation944588
display id: annotation944588
version: 1
top level: BBa_P0153
location: https://synbiohub.org/public/igem/BBa_P0153/annotation944588/range944588/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_P0153/annotation944588/range944588/1
type: Range
persistent identity: range944588
display id: range944588
version: 1
direction: direction/forward
top level: BBa_P0153
start: 741
end: 820
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation944598
display id: annotation944598
version: 1
top level: BBa_P0153
location: https://synbiohub.org/public/igem/BBa_P0153/annotation944598/range944598/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_P0153/annotation944598/range944598/1
type: Range
persistent identity: range944598
display id: range944598
version: 1
direction: direction/forward
top level: BBa_P0153
start: 829
end: 869
orientation: inline

BBa_C0053
type: SequenceAnnotation
persistent identity: annotation944580
display id: annotation944580
version: 1
top level: BBa_P0153
location: https://synbiohub.org/public/igem/BBa_P0153/annotation944580/range944580/1
component: BBa_C0053

https://synbiohub.org/public/igem/BBa_P0153/annotation944580/range944580/1
type: Range
persistent identity: range944580
display id: range944580
version: 1
direction: direction/forward
top level: BBa_P0153
start: 21
end: 707
orientation: inline

BBa_B0031
type: SequenceAnnotation
persistent identity: annotation944568
display id: annotation944568
version: 1
top level: BBa_P0153
location: https://synbiohub.org/public/igem/BBa_P0153/annotation944568/range944568/1
component: BBa_B0031

https://synbiohub.org/public/igem/BBa_P0153/annotation944568/range944568/1
type: Range
persistent identity: range944568
display id: range944568
version: 1
direction: direction/forward
top level: BBa_P0153
start: 1
end: 14
orientation: inline

BBa_K1031917
type: ComponentDefinition
persistent identity: BBa K1031917
display id: BBa_K1031917
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: J23117-XylS-Terminator
creator: He Shuaixin
created: 2013-10-13T11:00:00Z
modified: 2015-05-08T01:08:48Z
discontinued: false
dominant: false
group u list: _1338_
m user id: 0
owner id: 15843
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: 117-XylS-TT
mutable notes: molecular cloning
mutable provenance: Pseudomonas.putida
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: B0015
sequence annotation: XylS
sequence annotation: J23117
sequence: https://synbiohub.org/public/igem/BBa_K1031917_sequence/1

B0015
type: SequenceAnnotation
persistent identity: annotation2369602
display id: annotation2369602
version: 1
top level: BBa_K1031917
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1031917/annotation2369602/range2369602/1

https://synbiohub.org/public/igem/BBa_K1031917/annotation2369602/range2369602/1
type: Range
persistent identity: range2369602
display id: range2369602
version: 1
direction: direction/forward
top level: BBa_K1031917
start: 1035
end: 1163
orientation: inline

XylS
type: SequenceAnnotation
persistent identity: annotation2369601
display id: annotation2369601
version: 1
top level: BBa_K1031917
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1031917/annotation2369601/range2369601/1

https://synbiohub.org/public/igem/BBa_K1031917/annotation2369601/range2369601/1
type: Range
persistent identity: range2369601
display id: range2369601
version: 1
direction: direction/forward
top level: BBa_K1031917
start: 63
end: 1028
orientation: inline

J23117
type: SequenceAnnotation
persistent identity: annotation2369600
display id: annotation2369600
version: 1
top level: BBa_K1031917
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1031917/annotation2369600/range2369600/1

https://synbiohub.org/public/igem/BBa_K1031917/annotation2369600/range2369600/1
type: Range
persistent identity: range2369600
display id: range2369600
version: 1
direction: direction/forward
top level: BBa_K1031917
start: 1
end: 35
orientation: inline

BBa_P0452
type: ComponentDefinition
persistent identity: BBa P0452
display id: BBa_P0452
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PoPS -> cI (434) [S0154]
creator: Randy Rettberg
created: 2004-04-26T11:00:00Z
modified: 2015-05-08T01:14:11Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0012
component: BBa_B0034
component: BBa_C0052
component: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_B0010
sequence annotation: BBa_C0052
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_P0452_sequence/1

BBa_B0012
type: Component
persistent identity: component945016
display id: component945016
version: 1
top level: BBa_P0452
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0034
type: Component
persistent identity: component944989
display id: component944989
version: 1
top level: BBa_P0452
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_C0052
type: Component
persistent identity: component944999
display id: component944999
version: 1
top level: BBa_P0452
access: public
definition: cI 434

BBa_B0010
type: Component
persistent identity: component945006
display id: component945006
version: 1
top level: BBa_P0452
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation945016
display id: annotation945016
version: 1
top level: BBa_P0452
location: https://synbiohub.org/public/igem/BBa_P0452/annotation945016/range945016/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_P0452/annotation945016/range945016/1
type: Range
persistent identity: range945016
display id: range945016
version: 1
direction: direction/forward
top level: BBa_P0452
start: 809
end: 849
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation945006
display id: annotation945006
version: 1
top level: BBa_P0452
location: https://synbiohub.org/public/igem/BBa_P0452/annotation945006/range945006/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_P0452/annotation945006/range945006/1
type: Range
persistent identity: range945006
display id: range945006
version: 1
direction: direction/forward
top level: BBa_P0452
start: 721
end: 800
orientation: inline

BBa_C0052
type: SequenceAnnotation
persistent identity: annotation944999
display id: annotation944999
version: 1
top level: BBa_P0452
location: https://synbiohub.org/public/igem/BBa_P0452/annotation944999/range944999/1
component: BBa_C0052

https://synbiohub.org/public/igem/BBa_P0452/annotation944999/range944999/1
type: Range
persistent identity: range944999
display id: range944999
version: 1
direction: direction/forward
top level: BBa_P0452
start: 19
end: 687
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation944989
display id: annotation944989
version: 1
top level: BBa_P0452
location: https://synbiohub.org/public/igem/BBa_P0452/annotation944989/range944989/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_P0452/annotation944989/range944989/1
type: Range
persistent identity: range944989
display id: range944989
version: 1
direction: direction/forward
top level: BBa_P0452
start: 1
end: 12
orientation: inline

BBa_K544003
type: ComponentDefinition
persistent identity: BBa K544003
display id: BBa_K544003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Tetracycline Repressor with Consitutive Promoter BBa_J23116
creator: Leung Man Hong
created: 2011-09-29T11:00:00Z
modified: 2015-05-08T01:12:39Z
discontinued: false
dominant: false
experience: experience/None
group u list: _711_
m user id: 0
owner id: 6780
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: The tetR-HNS2-90 protein fuses the polymerization demain of histone-like nucleoid-structuring (H-NS) protein to tetR. Thus, in E.coli, when TetR-HNS2-90 binds the TetR-specific DNA motif, it will recruit native H-NS to bind to adjacent DNA, which may lead to local gene silencing. This fusion protein is driven by constitutive promoter family members (BBa-J23103, BBa-J23109, BBa-J23116, and BBa-J23106). Experimental data indicates that TetR-HNS2-90 is more effective than TetR on transcription repression.
mutable notes: The source of this part is based on the registry distribution
mutable provenance: The source of this part is based on the parts in the registry distribution
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: tet-R
sequence annotation: BBa_B0034
sequence annotation: BBa_J23116
sequence: https://synbiohub.org/public/igem/BBa_K544003_sequence/1

tet-R
type: SequenceAnnotation
persistent identity: annotation2147991
display id: annotation2147991
version: 1
top level: BBa_K544003
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K544003/annotation2147991/range2147991/1

https://synbiohub.org/public/igem/BBa_K544003/annotation2147991/range2147991/1
type: Range
persistent identity: range2147991
display id: range2147991
version: 1
direction: direction/forward
top level: BBa_K544003
start: 62
end: 682
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2147990
display id: annotation2147990
version: 1
top level: BBa_K544003
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K544003/annotation2147990/range2147990/1

https://synbiohub.org/public/igem/BBa_K544003/annotation2147990/range2147990/1
type: Range
persistent identity: range2147990
display id: range2147990
version: 1
direction: direction/forward
top level: BBa_K544003
start: 44
end: 55
orientation: inline

BBa_J23116
type: SequenceAnnotation
persistent identity: annotation2147989
display id: annotation2147989
version: 1
top level: BBa_K544003
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K544003/annotation2147989/range2147989/1

https://synbiohub.org/public/igem/BBa_K544003/annotation2147989/range2147989/1
type: Range
persistent identity: range2147989
display id: range2147989
version: 1
direction: direction/forward
top level: BBa_K544003
start: 1
end: 35
orientation: inline

BBa_K545007
type: ComponentDefinition
persistent identity: BBa K545007
display id: BBa_K545007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: rsmA repressor
creator: Grenoble 2011
created: 2011-09-19T11:00:00Z
modified: 2015-05-08T01:12:39Z
discontinued: false
dominant: true
experience: experience/None
group u list: _712_
m user id: 0
owner id: 8648
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: RsmA is a transcription repressor protein who fixes fha or mag RBS. The transcription is recovered by the rsmY iRNA which binds the rsmA protein.
mutable notes: This part was build following the standard construction protocol.
mutable provenance: Pseudomonas aeruginosa
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: misc
sequence: https://synbiohub.org/public/igem/BBa_K545007_sequence/1

misc
type: SequenceAnnotation
persistent identity: annotation2137092
display id: annotation2137092
version: 1
top level: BBa_K545007
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K545007/annotation2137092/range2137092/1

https://synbiohub.org/public/igem/BBa_K545007/annotation2137092/range2137092/1
type: Range
persistent identity: range2137092
display id: range2137092
version: 1
direction: direction/forward
top level: BBa_K545007
start: 1
end: 186
orientation: inline

Lov-tetR
type: ComponentDefinition
persistent identity: BBa K785000
display id: BBa_K785000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Lov-tetR (light sensor with Ptet repression) light->PtetO repressed
creator: Zining Hou
created: 2012-09-20T11:00:00Z
modified: 2015-05-08T01:13:22Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1040_
m user id: 0
owner id: 11000
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This part code a protein as a light sensor and repressor. With the existence of this protein, expression downstream the PtetO promoter will be repressed under the 450nm blue light. This protein, which is actually a well designed fusion protein, include two domain. The Lov domain is from Avena sativa phototrophin1(AsLOV2). The tetR is from the tetracycline resistance, which could combine the tetracycline and enable the expression of the tetA. We carefully designed the linker between these two parts, and fulfilled our sensor part in our biowave circuit.
mutable notes: This part is designed for our biowave project.
mutable provenance: This coding sequence is form the Avena sativa phototrophin1(AsLOV2) and tetracycline resistance. The whole gene is synthesized by Invitrogen company.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: linker part
sequence annotation: tetR DNA binding domain
sequence annotation: Lov light sensor domain
sequence: https://synbiohub.org/public/igem/BBa_K785000_sequence/1

linker part
type: SequenceAnnotation
persistent identity: annotation2194042
display id: annotation2194042
version: 1
top level: Lov-tetR
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K785000/annotation2194042/range2194042/1

https://synbiohub.org/public/igem/BBa_K785000/annotation2194042/range2194042/1
type: Range
persistent identity: range2194042
display id: range2194042
version: 1
direction: direction/forward
top level: Lov-tetR
start: 213
end: 345
orientation: inline

tetR DNA binding domain
type: SequenceAnnotation
persistent identity: annotation2194043
display id: annotation2194043
version: 1
top level: Lov-tetR
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K785000/annotation2194043/range2194043/1

https://synbiohub.org/public/igem/BBa_K785000/annotation2194043/range2194043/1
type: Range
persistent identity: range2194043
display id: range2194043
version: 1
direction: direction/forward
top level: Lov-tetR
start: 294
end: 1044
orientation: inline

Lov light sensor domain
type: SequenceAnnotation
persistent identity: annotation2194041
display id: annotation2194041
version: 1
top level: Lov-tetR
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K785000/annotation2194041/range2194041/1

https://synbiohub.org/public/igem/BBa_K785000/annotation2194041/range2194041/1
type: Range
persistent identity: range2194041
display id: range2194041
version: 1
direction: direction/forward
top level: Lov-tetR
start: 1
end: 294
orientation: inline

BBa_J24676
type: ComponentDefinition
persistent identity: BBa J24676
display id: BBa_J24676
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pBAD to tetR coding region, lacI coding region, and mCherry reporter
creator: Annie Gao
created: 2006-10-25T11:00:00Z
modified: 2015-08-31T04:08:41Z
discontinued: false
dominant: false
experience: experience/None
group u list: _58_
m user id: 0
owner id: 1017
owning group id: 58
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: Links the pBAD promoter, which is induced by arabinose and inhibited by araC, to a tetR coding region with an RBS, a LacI coding region with an RBS, and an mCherry reporter. Activation of this pathway results in tetR production, lacI production, and mCherry production.
mutable notes: Strength of RBS may affect the strength of output.
mutable provenance: Composite of other iGEM parts
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_S03518
component: BBa_S03520
component: BBa_I0500
component: BBa_J06702
sequence annotation: BBa_J06702
sequence annotation: BBa_S03520
sequence annotation: BBa_I0500
sequence annotation: BBa_S03518
sequence: https://synbiohub.org/public/igem/BBa_J24676_sequence/1

BBa_S03518
type: Component
persistent identity: component2274563
display id: component2274563
version: 1
top level: BBa_J24676
access: public
definition: https://synbiohub.org/public/igem/BBa_S03518/1

BBa_S03520
type: Component
persistent identity: component2274570
display id: component2274570
version: 1
top level: BBa_J24676
access: public
definition: https://synbiohub.org/public/igem/BBa_S03520/1

BBa_I0500
type: Component
persistent identity: component2274556
display id: component2274556
version: 1
top level: BBa_J24676
access: public
definition: https://synbiohub.org/public/igem/BBa_I0500/1

BBa_J06702
type: Component
persistent identity: component2274582
display id: component2274582
version: 1
top level: BBa_J24676
access: public
definition: https://synbiohub.org/public/igem/BBa_J06702/1

BBa_J06702
type: SequenceAnnotation
persistent identity: annotation2274582
display id: annotation2274582
version: 1
top level: BBa_J24676
location: https://synbiohub.org/public/igem/BBa_J24676/annotation2274582/range2274582/1
component: BBa_J06702

https://synbiohub.org/public/igem/BBa_J24676/annotation2274582/range2274582/1
type: Range
persistent identity: range2274582
display id: range2274582
version: 1
direction: direction/reverse
top level: BBa_J24676
start: 3109
end: 3977
orientation: inline

BBa_S03520
type: SequenceAnnotation
persistent identity: annotation2274570
display id: annotation2274570
version: 1
top level: BBa_J24676
location: https://synbiohub.org/public/igem/BBa_J24676/annotation2274570/range2274570/1
component: BBa_S03520

https://synbiohub.org/public/igem/BBa_J24676/annotation2274570/range2274570/1
type: Range
persistent identity: range2274570
display id: range2274570
version: 1
direction: direction/reverse
top level: BBa_J24676
start: 1930
end: 3100
orientation: inline

BBa_I0500
type: SequenceAnnotation
persistent identity: annotation2274556
display id: annotation2274556
version: 1
top level: BBa_J24676
location: https://synbiohub.org/public/igem/BBa_J24676/annotation2274556/range2274556/1
component: BBa_I0500

https://synbiohub.org/public/igem/BBa_J24676/annotation2274556/range2274556/1
type: Range
persistent identity: range2274556
display id: range2274556
version: 1
direction: direction/reverse
top level: BBa_J24676
start: 1
end: 1210
orientation: inline

BBa_S03518
type: SequenceAnnotation
persistent identity: annotation2274563
display id: annotation2274563
version: 1
top level: BBa_J24676
location: https://synbiohub.org/public/igem/BBa_J24676/annotation2274563/range2274563/1
component: BBa_S03518

https://synbiohub.org/public/igem/BBa_J24676/annotation2274563/range2274563/1
type: Range
persistent identity: range2274563
display id: range2274563
version: 1
direction: direction/reverse
top level: BBa_J24676
start: 1219
end: 1921
orientation: inline

BBa_K165082
type: ComponentDefinition
persistent identity: BBa K165082
display id: BBa_K165082
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pMET25 + Untagged LexA activator on pRS305
creator: John Szymanski
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:10:57Z
discontinued: false
dominant: false
group u list: _267_
m user id: 0
owner id: 2512
owning group id: 58
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: -
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_K165000
component: BBa_K165020
component: BBa_K165002
sequence annotation: BBa_K165002
sequence annotation: BBa_K165000
sequence annotation: BBa_K165020
sequence: https://synbiohub.org/public/igem/BBa_K165082_sequence/1

BBa_K165000
type: Component
persistent identity: component1997139
display id: component1997139
version: 1
top level: BBa_K165082
access: public
definition: https://synbiohub.org/public/igem/BBa_K165000/1

BBa_K165020
type: Component
persistent identity: component1997141
display id: component1997141
version: 1
top level: BBa_K165082
access: public
definition: lexA

BBa_K165002
type: Component
persistent identity: component1997140
display id: component1997140
version: 1
top level: BBa_K165082
access: public
definition: https://synbiohub.org/public/igem/BBa_K165002/1

BBa_K165002
type: SequenceAnnotation
persistent identity: annotation1997140
display id: annotation1997140
version: 1
top level: BBa_K165082
location: https://synbiohub.org/public/igem/BBa_K165082/annotation1997140/range1997140/1
component: BBa_K165002

https://synbiohub.org/public/igem/BBa_K165082/annotation1997140/range1997140/1
type: Range
persistent identity: range1997140
display id: range1997140
version: 1
direction: direction/reverse
top level: BBa_K165082
start: 396
end: 413
orientation: inline

BBa_K165000
type: SequenceAnnotation
persistent identity: annotation1997139
display id: annotation1997139
version: 1
top level: BBa_K165082
location: https://synbiohub.org/public/igem/BBa_K165082/annotation1997139/range1997139/1
component: BBa_K165000

https://synbiohub.org/public/igem/BBa_K165082/annotation1997139/range1997139/1
type: Range
persistent identity: range1997139
display id: range1997139
version: 1
direction: direction/reverse
top level: BBa_K165082
start: 1
end: 387
orientation: inline

BBa_K165020
type: SequenceAnnotation
persistent identity: annotation1997141
display id: annotation1997141
version: 1
top level: BBa_K165082
location: https://synbiohub.org/public/igem/BBa_K165082/annotation1997141/range1997141/1
component: BBa_K165020

https://synbiohub.org/public/igem/BBa_K165082/annotation1997141/range1997141/1
type: Range
persistent identity: range1997141
display id: range1997141
version: 1
direction: direction/reverse
top level: BBa_K165082
start: 422
end: 1093
orientation: inline

BBa_K082022
type: ComponentDefinition
persistent identity: BBa K082022
display id: BBa_K082022
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: B0034-C0051-B0015
creator: Yanting Xue , He Shen
created: 2008-10-28T12:00:00Z
modified: 2015-05-08T01:08:35Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _228_
m user id: 0
owner id: 1560
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: none
mutable notes: none
mutable provenance: none
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_C0051
component: BBa_B0034
component: BBa_B0015
sequence annotation: BBa_B0034
sequence annotation: BBa_B0015
sequence annotation: BBa_C0051
sequence: https://synbiohub.org/public/igem/BBa_K082022_sequence/1

BBa_C0051
type: Component
persistent identity: component2249420
display id: component2249420
version: 1
top level: BBa_K082022
access: public
definition: cI lam

BBa_B0034
type: Component
persistent identity: component2249416
display id: component2249416
version: 1
top level: BBa_K082022
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0015
type: Component
persistent identity: component2249427
display id: component2249427
version: 1
top level: BBa_K082022
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2249416
display id: annotation2249416
version: 1
top level: BBa_K082022
location: https://synbiohub.org/public/igem/BBa_K082022/annotation2249416/range2249416/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K082022/annotation2249416/range2249416/1
type: Range
persistent identity: range2249416
display id: range2249416
version: 1
direction: direction/reverse
top level: BBa_K082022
start: 1
end: 12
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2249427
display id: annotation2249427
version: 1
top level: BBa_K082022
location: https://synbiohub.org/public/igem/BBa_K082022/annotation2249427/range2249427/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K082022/annotation2249427/range2249427/1
type: Range
persistent identity: range2249427
display id: range2249427
version: 1
direction: direction/reverse
top level: BBa_K082022
start: 802
end: 930
orientation: inline

BBa_C0051
type: SequenceAnnotation
persistent identity: annotation2249420
display id: annotation2249420
version: 1
top level: BBa_K082022
location: https://synbiohub.org/public/igem/BBa_K082022/annotation2249420/range2249420/1
component: BBa_C0051

https://synbiohub.org/public/igem/BBa_K082022/annotation2249420/range2249420/1
type: Range
persistent identity: range2249420
display id: range2249420
version: 1
direction: direction/reverse
top level: BBa_K082022
start: 19
end: 793
orientation: inline

BBa_I726005
type: ComponentDefinition
persistent identity: BBa I726005
display id: BBa_I726005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Trc-LC
creator: Bangalore iGEM
created: 2007-07-03T11:00:00Z
modified: 2015-08-31T04:07:55Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _107_
m user id: 0
owner id: 2077
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: A similar part is available in registry. we designed this as we faced some problems in CFP expression with the earlier part. we have tested this part and it is functional.
mutable notes: none
mutable provenance: Registry
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_E0020
component: BBa_B0034
component: BBa_R0011
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0034
sequence annotation: BBa_R0011
sequence annotation: BBa_E0020
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_I726005_sequence/1

BBa_E0020
type: Component
persistent identity: component1936432
display id: component1936432
version: 1
top level: BBa_I726005
access: public
definition: https://synbiohub.org/public/igem/BBa_E0020/1

BBa_B0034
type: Component
persistent identity: component1936431
display id: component1936431
version: 1
top level: BBa_I726005
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_R0011
type: Component
persistent identity: component1936425
display id: component1936425
version: 1
top level: BBa_I726005
access: public
definition: https://synbiohub.org/public/igem/BBa_R0011/1

BBa_B0012
type: Component
persistent identity: component1936435
display id: component1936435
version: 1
top level: BBa_I726005
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0010
type: Component
persistent identity: component1936433
display id: component1936433
version: 1
top level: BBa_I726005
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1936431
display id: annotation1936431
version: 1
top level: BBa_I726005
location: https://synbiohub.org/public/igem/BBa_I726005/annotation1936431/range1936431/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I726005/annotation1936431/range1936431/1
type: Range
persistent identity: range1936431
display id: range1936431
version: 1
direction: direction/reverse
top level: BBa_I726005
start: 64
end: 75
orientation: inline

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation1936425
display id: annotation1936425
version: 1
top level: BBa_I726005
location: https://synbiohub.org/public/igem/BBa_I726005/annotation1936425/range1936425/1
component: BBa_R0011

https://synbiohub.org/public/igem/BBa_I726005/annotation1936425/range1936425/1
type: Range
persistent identity: range1936425
display id: range1936425
version: 1
direction: direction/forward
top level: BBa_I726005
start: 1
end: 54
orientation: inline

BBa_E0020
type: SequenceAnnotation
persistent identity: annotation1936432
display id: annotation1936432
version: 1
top level: BBa_I726005
location: https://synbiohub.org/public/igem/BBa_I726005/annotation1936432/range1936432/1
component: BBa_E0020

https://synbiohub.org/public/igem/BBa_I726005/annotation1936432/range1936432/1
type: Range
persistent identity: range1936432
display id: range1936432
version: 1
direction: direction/reverse
top level: BBa_I726005
start: 82
end: 804
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1936433
display id: annotation1936433
version: 1
top level: BBa_I726005
location: https://synbiohub.org/public/igem/BBa_I726005/annotation1936433/range1936433/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I726005/annotation1936433/range1936433/1
type: Range
persistent identity: range1936433
display id: range1936433
version: 1
direction: direction/forward
top level: BBa_I726005
start: 813
end: 892
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1936435
display id: annotation1936435
version: 1
top level: BBa_I726005
location: https://synbiohub.org/public/igem/BBa_I726005/annotation1936435/range1936435/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I726005/annotation1936435/range1936435/1
type: Range
persistent identity: range1936435
display id: range1936435
version: 1
direction: direction/forward
top level: BBa_I726005
start: 901
end: 941
orientation: inline

araC
type: ComponentDefinition
persistent identity: BBa C0080
display id: BBa_C0080
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: araC arabinose operon regulatory protein (repressor/activator) from E. coli (+LVA)
creator: Sara Neves (Fighting Darwins)
created: 2004-01-27T12:00:00Z
modified: 2015-08-31T04:07:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: coding region for the araC gene, used to make araC protein for use in positive or negative regulation of TIPs output (see also R0080, R0081)
mutable provenance: GenBank: NC_002655 (www.ncbi.nlm.nih.gov)
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: LVA
sequence annotation: araC
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C0080_sequence/1

LVA
type: SequenceAnnotation
persistent identity: annotation308196
display id: annotation308196
version: 1
top level: araC
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_C0080/annotation308196/range308196/1

https://synbiohub.org/public/igem/BBa_C0080/annotation308196/range308196/1
type: Range
persistent identity: range308196
display id: range308196
version: 1
direction: direction/forward
top level: araC
start: 877
end: 909
orientation: inline

araC
type: SequenceAnnotation
persistent identity: annotation308191
display id: annotation308191
version: 1
top level: araC
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_C0080/annotation308191/range308191/1

https://synbiohub.org/public/igem/BBa_C0080/annotation308191/range308191/1
type: Range
persistent identity: range308191
display id: range308191
version: 1
direction: direction/forward
top level: araC
start: 1
end: 876
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2214004
display id: annotation2214004
version: 1
top level: araC
role: SO:0000807
role: feature/barcode
location: https://synbiohub.org/public/igem/BBa_C0080/annotation2214004/range2214004/1

https://synbiohub.org/public/igem/BBa_C0080/annotation2214004/range2214004/1
type: Range
persistent identity: range2214004
display id: range2214004
version: 1
direction: direction/forward
top level: araC
start: 916
end: 940
orientation: inline

BBa_K772104
type: ComponentDefinition
persistent identity: BBa K772104
display id: BBa_K772104
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pLac IPTG inducible generator
creator: Mustafa Elitok
created: 2012-10-04T11:00:00Z
modified: 2015-05-08T01:13:15Z
discontinued: false
dominant: false
group u list: _1024_
m user id: 0
owner id: 9573
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This part is an inverting regulator sensitive to LacI and CAP. It contains two protein binding sites. The first binds the CAP protein, which is generally present in E.coli and is asocciated with cell health and availability of glucose. The second binds LacI protein. This part also produces LacI protein as an oscillator which results that as LacI protein is synthesized, the promoter wil be inhibited until the presence of IPTG. The inhibition will decrease the production of LacI which leads to the synthesis of this inhibitor. >In the absence of LacI protein and CAP protein, this part promotes transcription. >In the presence of LacI protein and CAP protein, this part inhibits transcription. >LacI can be inhibited by IPTG. >LacI is coded by BBa_C0010
mutable notes: Note that this part is not working unless IPTG is present in the media.
mutable provenance: Registry
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
component: BBa_J04500
component: BBa_C0012
sequence annotation: BBa_C0012
sequence annotation: BBa_J04500
sequence: https://synbiohub.org/public/igem/BBa_K772104_sequence/1

BBa_J04500
type: Component
persistent identity: component2248259
display id: component2248259
version: 1
top level: BBa_K772104
access: public
definition: https://synbiohub.org/public/igem/BBa_J04500/1

BBa_C0012
type: Component
persistent identity: component2248261
display id: component2248261
version: 1
top level: BBa_K772104
access: public
definition: lacI

BBa_C0012
type: SequenceAnnotation
persistent identity: annotation2248261
display id: annotation2248261
version: 1
top level: BBa_K772104
location: https://synbiohub.org/public/igem/BBa_K772104/annotation2248261/range2248261/1
component: BBa_C0012

https://synbiohub.org/public/igem/BBa_K772104/annotation2248261/range2248261/1
type: Range
persistent identity: range2248261
display id: range2248261
version: 1
direction: direction/forward
top level: BBa_K772104
start: 227
end: 1354
orientation: inline

BBa_J04500
type: SequenceAnnotation
persistent identity: annotation2248259
display id: annotation2248259
version: 1
top level: BBa_K772104
location: https://synbiohub.org/public/igem/BBa_K772104/annotation2248259/range2248259/1
component: BBa_J04500

https://synbiohub.org/public/igem/BBa_K772104/annotation2248259/range2248259/1
type: Range
persistent identity: range2248259
display id: range2248259
version: 1
direction: direction/reverse
top level: BBa_K772104
start: 1
end: 220
orientation: inline

BBa_K1520004
type: ComponentDefinition
persistent identity: BBa K1520004
display id: BBa_K1520004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Prlux-rbs-lacI-Ter
creator: yue wu
created: 2014-09-21T11:00:00Z
modified: 2015-05-08T01:10:48Z
discontinued: false
dominant: false
group u list: _1902_
m user id: 0
owner id: 21318
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Prlux-rbs-lacI-Ter. It worked out an expression of lacI in the presence of luxR and AHL.
mutable notes: None
mutable provenance: Prlux, lacI, Ter
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_R0062
component: BBa_K1520029
component: BBa_K1520502
sequence annotation: BBa_R0062
sequence annotation: BBa_K1520502
sequence annotation: BBa_K1520029
sequence: https://synbiohub.org/public/igem/BBa_K1520004_sequence/1

BBa_R0062
type: Component
persistent identity: component2384890
display id: component2384890
version: 1
top level: BBa_K1520004
access: public
definition: https://synbiohub.org/public/igem/BBa_R0062/1

BBa_K1520029
type: Component
persistent identity: component2384898
display id: component2384898
version: 1
top level: BBa_K1520004
access: public
definition: https://synbiohub.org/public/igem/BBa_K1520029/1

BBa_K1520502
type: Component
persistent identity: component2384897
display id: component2384897
version: 1
top level: BBa_K1520004
access: public
definition: BBa_K1520502

BBa_R0062
type: SequenceAnnotation
persistent identity: annotation2384890
display id: annotation2384890
version: 1
top level: BBa_K1520004
location: https://synbiohub.org/public/igem/BBa_K1520004/annotation2384890/range2384890/1
component: BBa_R0062

https://synbiohub.org/public/igem/BBa_K1520004/annotation2384890/range2384890/1
type: Range
persistent identity: range2384890
display id: range2384890
version: 1
direction: direction/forward
top level: BBa_K1520004
start: 1
end: 55
orientation: inline

BBa_K1520502
type: SequenceAnnotation
persistent identity: annotation2384897
display id: annotation2384897
version: 1
top level: BBa_K1520004
location: https://synbiohub.org/public/igem/BBa_K1520004/annotation2384897/range2384897/1
component: BBa_K1520502

https://synbiohub.org/public/igem/BBa_K1520004/annotation2384897/range2384897/1
type: Range
persistent identity: range2384897
display id: range2384897
version: 1
direction: direction/reverse
top level: BBa_K1520004
start: 64
end: 1234
orientation: inline

BBa_K1520029
type: SequenceAnnotation
persistent identity: annotation2384898
display id: annotation2384898
version: 1
top level: BBa_K1520004
location: https://synbiohub.org/public/igem/BBa_K1520004/annotation2384898/range2384898/1
component: BBa_K1520029

https://synbiohub.org/public/igem/BBa_K1520004/annotation2384898/range2384898/1
type: Range
persistent identity: range2384898
display id: range2384898
version: 1
direction: direction/reverse
top level: BBa_K1520004
start: 1243
end: 1371
orientation: inline

BBa_K165101
type: ComponentDefinition
persistent identity: BBa K165101
display id: BBa_K165101
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Zif268-HIV bs + LexA bs + mCYC + Zif268-HIV repressor (mCherryx2 tagged) on pRS304*
creator: John Szymanski
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:10:57Z
discontinued: false
dominant: true
group u list: _267_
m user id: 0
owner id: 2512
owning group id: 58
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: -
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_K165011
component: BBa_K165002
component: BBa_K165004
component: BBa_K165006
component: BBa_K165003
component: BBa_K165018
component: BBa_K165031
sequence annotation: BBa_K165006
sequence annotation: BBa_K165004
sequence annotation: BBa_K165003
sequence annotation: BBa_K165018
sequence annotation: BBa_K165002
sequence annotation: BBa_K165031
sequence annotation: BBa_K165011
sequence: https://synbiohub.org/public/igem/BBa_K165101_sequence/1

BBa_K165011
type: Component
persistent identity: component1997405
display id: component1997405
version: 1
top level: BBa_K165101
access: public
definition: https://synbiohub.org/public/igem/BBa_K165011/1

BBa_K165002
type: Component
persistent identity: component1997407
display id: component1997407
version: 1
top level: BBa_K165101
access: public
definition: https://synbiohub.org/public/igem/BBa_K165002/1

BBa_K165004
type: Component
persistent identity: component1997411
display id: component1997411
version: 1
top level: BBa_K165101
access: public
definition: https://synbiohub.org/public/igem/BBa_K165004/1

BBa_K165006
type: Component
persistent identity: component1997409
display id: component1997409
version: 1
top level: BBa_K165101
access: public
definition: https://synbiohub.org/public/igem/BBa_K165006/1

BBa_K165003
type: Component
persistent identity: component1997408
display id: component1997408
version: 1
top level: BBa_K165101
access: public
definition: https://synbiohub.org/public/igem/BBa_K165003/1

BBa_K165004
type: Component
persistent identity: component1997410
display id: component1997410
version: 1
top level: BBa_K165101
access: public
definition: https://synbiohub.org/public/igem/BBa_K165004/1

BBa_K165018
type: Component
persistent identity: component1997412
display id: component1997412
version: 1
top level: BBa_K165101
access: public
definition: https://synbiohub.org/public/igem/BBa_K165018/1

BBa_K165031
type: Component
persistent identity: component1997406
display id: component1997406
version: 1
top level: BBa_K165101
access: public
definition: https://synbiohub.org/public/igem/BBa_K165031/1

BBa_K165006
type: SequenceAnnotation
persistent identity: annotation1997409
display id: annotation1997409
version: 1
top level: BBa_K165101
location: https://synbiohub.org/public/igem/BBa_K165101/annotation1997409/range1997409/1
component: BBa_K165006

https://synbiohub.org/public/igem/BBa_K165101/annotation1997409/range1997409/1
type: Range
persistent identity: range1997409
display id: range1997409
version: 1
direction: direction/reverse
top level: BBa_K165101
start: 3311
end: 3562
orientation: inline

BBa_K165004
type: SequenceAnnotation
persistent identity: annotation1997410
display id: annotation1997410
version: 1
top level: BBa_K165101
location: https://synbiohub.org/public/igem/BBa_K165101/annotation1997410/range1997410/1
component: BBa_K165004

https://synbiohub.org/public/igem/BBa_K165101/annotation1997410/range1997410/1
type: Range
persistent identity: range1997410
display id: range1997410
version: 1
direction: direction/reverse
top level: BBa_K165101
start: 3571
end: 4305
orientation: inline

BBa_K165003
type: SequenceAnnotation
persistent identity: annotation1997408
display id: annotation1997408
version: 1
top level: BBa_K165101
location: https://synbiohub.org/public/igem/BBa_K165101/annotation1997408/range1997408/1
component: BBa_K165003

https://synbiohub.org/public/igem/BBa_K165101/annotation1997408/range1997408/1
type: Range
persistent identity: range1997408
display id: range1997408
version: 1
direction: direction/reverse
top level: BBa_K165101
start: 492
end: 3302
orientation: inline

BBa_K165018
type: SequenceAnnotation
persistent identity: annotation1997412
display id: annotation1997412
version: 1
top level: BBa_K165101
location: https://synbiohub.org/public/igem/BBa_K165101/annotation1997412/range1997412/1
component: BBa_K165018

https://synbiohub.org/public/igem/BBa_K165101/annotation1997412/range1997412/1
type: Range
persistent identity: range1997412
display id: range1997412
version: 1
direction: direction/reverse
top level: BBa_K165101
start: 5057
end: 5309
orientation: inline

BBa_K165002
type: SequenceAnnotation
persistent identity: annotation1997407
display id: annotation1997407
version: 1
top level: BBa_K165101
location: https://synbiohub.org/public/igem/BBa_K165101/annotation1997407/range1997407/1
component: BBa_K165002

https://synbiohub.org/public/igem/BBa_K165101/annotation1997407/range1997407/1
type: Range
persistent identity: range1997407
display id: range1997407
version: 1
direction: direction/reverse
top level: BBa_K165101
start: 466
end: 483
orientation: inline

BBa_K165031
type: SequenceAnnotation
persistent identity: annotation1997406
display id: annotation1997406
version: 1
top level: BBa_K165101
location: https://synbiohub.org/public/igem/BBa_K165101/annotation1997406/range1997406/1
component: BBa_K165031

https://synbiohub.org/public/igem/BBa_K165101/annotation1997406/range1997406/1
type: Range
persistent identity: range1997406
display id: range1997406
version: 1
direction: direction/reverse
top level: BBa_K165101
start: 55
end: 457
orientation: inline

BBa_K165011
type: SequenceAnnotation
persistent identity: annotation1997405
display id: annotation1997405
version: 1
top level: BBa_K165101
location: https://synbiohub.org/public/igem/BBa_K165101/annotation1997405/range1997405/1
component: BBa_K165011

https://synbiohub.org/public/igem/BBa_K165101/annotation1997405/range1997405/1
type: Range
persistent identity: range1997405
display id: range1997405
version: 1
direction: direction/reverse
top level: BBa_K165101
start: 1
end: 46
orientation: inline

BBa_K165004
type: SequenceAnnotation
persistent identity: annotation1997411
display id: annotation1997411
version: 1
top level: BBa_K165101
location: https://synbiohub.org/public/igem/BBa_K165101/annotation1997411/range1997411/1
component: BBa_K165004

https://synbiohub.org/public/igem/BBa_K165101/annotation1997411/range1997411/1
type: Range
persistent identity: range1997411
display id: range1997411
version: 1
direction: direction/reverse
top level: BBa_K165101
start: 4314
end: 5048
orientation: inline

BBa_K091182
type: ComponentDefinition
persistent identity: BBa K091182
display id: BBa_K091182
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pLasR/cI-RBS-LuxI-RBS-Mnt-TT-pMnt/tetR-RBS-LuxI-RBS-cI-TT
creator: Robert Cool
created: 2008-08-04T11:00:00Z
modified: 2015-05-08T01:08:38Z
discontinued: false
dominant: false
experience: experience/Issues
group u list: _191_
m user id: 0
owner id: 3080
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This part is a combination of the two parts K091155 and K091144.
mutable notes: no spceial considerations
mutable provenance: These two parts contain parts that are in the registry and DNA built from oligos.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_K091155
component: BBa_K091144
sequence annotation: BBa_K091155
sequence annotation: BBa_K091144
sequence: https://synbiohub.org/public/igem/BBa_K091182_sequence/1

BBa_K091155
type: Component
persistent identity: component2301908
display id: component2301908
version: 1
top level: BBa_K091182
access: public
definition: https://synbiohub.org/public/igem/BBa_K091155/1

BBa_K091144
type: Component
persistent identity: component2301935
display id: component2301935
version: 1
top level: BBa_K091182
access: public
definition: https://synbiohub.org/public/igem/BBa_K091144/1

BBa_K091155
type: SequenceAnnotation
persistent identity: annotation2301908
display id: annotation2301908
version: 1
top level: BBa_K091182
location: https://synbiohub.org/public/igem/BBa_K091182/annotation2301908/range2301908/1
component: BBa_K091155

https://synbiohub.org/public/igem/BBa_K091182/annotation2301908/range2301908/1
type: Range
persistent identity: range2301908
display id: range2301908
version: 1
direction: direction/reverse
top level: BBa_K091182
start: 1
end: 1309
orientation: inline

BBa_K091144
type: SequenceAnnotation
persistent identity: annotation2301935
display id: annotation2301935
version: 1
top level: BBa_K091182
location: https://synbiohub.org/public/igem/BBa_K091182/annotation2301935/range2301935/1
component: BBa_K091144

https://synbiohub.org/public/igem/BBa_K091182/annotation2301935/range2301935/1
type: Range
persistent identity: range2301935
display id: range2301935
version: 1
direction: direction/reverse
top level: BBa_K091182
start: 1318
end: 2988
orientation: inline

BBa_K082026
type: ComponentDefinition
persistent identity: BBa K082026
display id: BBa_K082026
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: B0034-LacIM1-B0015
creator: Yanting Xue , He Shen
created: 2008-10-28T12:00:00Z
modified: 2015-05-08T01:08:36Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _228_
m user id: 0
owner id: 1560
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: none
mutable notes: none
mutable provenance: none
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0034
component: BBa_K082005
component: BBa_B0015
sequence annotation: BBa_K082005
sequence annotation: BBa_B0015
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K082026_sequence/1

BBa_B0034
type: Component
persistent identity: component2220455
display id: component2220455
version: 1
top level: BBa_K082026
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K082005
type: Component
persistent identity: component2220456
display id: component2220456
version: 1
top level: BBa_K082026
access: public
definition: https://synbiohub.org/public/igem/BBa_K082005/1

BBa_B0015
type: Component
persistent identity: component2220463
display id: component2220463
version: 1
top level: BBa_K082026
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_K082005
type: SequenceAnnotation
persistent identity: annotation2220456
display id: annotation2220456
version: 1
top level: BBa_K082026
location: https://synbiohub.org/public/igem/BBa_K082026/annotation2220456/range2220456/1
component: BBa_K082005

https://synbiohub.org/public/igem/BBa_K082026/annotation2220456/range2220456/1
type: Range
persistent identity: range2220456
display id: range2220456
version: 1
direction: direction/reverse
top level: BBa_K082026
start: 19
end: 1101
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2220463
display id: annotation2220463
version: 1
top level: BBa_K082026
location: https://synbiohub.org/public/igem/BBa_K082026/annotation2220463/range2220463/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K082026/annotation2220463/range2220463/1
type: Range
persistent identity: range2220463
display id: range2220463
version: 1
direction: direction/reverse
top level: BBa_K082026
start: 1110
end: 1238
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2220455
display id: annotation2220455
version: 1
top level: BBa_K082026
location: https://synbiohub.org/public/igem/BBa_K082026/annotation2220455/range2220455/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K082026/annotation2220455/range2220455/1
type: Range
persistent identity: range2220455
display id: range2220455
version: 1
direction: direction/reverse
top level: BBa_K082026
start: 1
end: 12
orientation: inline

BBa_K1031444
type: ComponentDefinition
persistent identity: BBa K1031444
display id: BBa_K1031444
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: J23114-HcaR
creator: He Shuaixin
created: 2013-09-08T11:00:00Z
modified: 2015-05-08T01:08:47Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1338_
m user id: 0
owner id: 15843
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: 114-HcaR-TT
mutable notes: molecular cloning
mutable provenance: PCR genome E.coli
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: BBa_J23114
sequence annotation: BBa_B0034
sequence annotation: HcaR
sequence: https://synbiohub.org/public/igem/BBa_K1031444_sequence/1

BBa_J23114
type: SequenceAnnotation
persistent identity: annotation2357463
display id: annotation2357463
version: 1
top level: BBa_K1031444
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1031444/annotation2357463/range2357463/1

https://synbiohub.org/public/igem/BBa_K1031444/annotation2357463/range2357463/1
type: Range
persistent identity: range2357463
display id: range2357463
version: 1
direction: direction/forward
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role: feature/rbs
role: SO:0000139
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HcaR
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role: feature/cds
role: SO:0000316
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BBa_K1897005
type: ComponentDefinition
persistent identity: BBa K1897005
display id: BBa_K1897005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: HasI sigma factor coding sequence
creator: Choi Yan Ru
created: 2016-10-08T11:00:00Z
modified: 2016-10-18T01:29:32Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2363_
m user id: 29428
owner id: 29428
owning group id: 9
status: status/Planning
bookmark: false
mutable description: HasI is the sigma factor under the has operon system
mutable notes: NIL
mutable provenance: The HasI gene is originally found in the Serratia marcescens. We obtained the gene sequence from the National Center for Biotechnology Information (NCBI) database (http://www.ncbi.nlm.nih.gov/nuccore/X81195.2) and synthesised the gene
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
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BBa_P0353
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persistent identity: BBa P0353
display id: BBa_P0353
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PoPS -> cII (p22) [S0161]
creator: Randy Rettberg
created: 2004-04-26T11:00:00Z
modified: 2015-05-08T01:14:10Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0010
component: BBa_B0033
component: BBa_B0012
component: BBa_C0053
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_C0053
sequence annotation: BBa_B0033
sequence: https://synbiohub.org/public/igem/BBa_P0353_sequence/1

BBa_B0010
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display id: component944811
version: 1
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access: public
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BBa_B0033
type: Component
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display id: component944790
version: 1
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BBa_B0012
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display id: component944821
version: 1
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access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_C0053
type: Component
persistent identity: component944803
display id: component944803
version: 1
top level: BBa_P0353
access: public
definition: c2 P22

BBa_B0010
type: SequenceAnnotation
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display id: annotation944811
version: 1
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BBa_B0012
type: SequenceAnnotation
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version: 1
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BBa_C0053
type: SequenceAnnotation
persistent identity: annotation944803
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version: 1
top level: BBa_P0353
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persistent identity: range944803
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BBa_B0033
type: SequenceAnnotation
persistent identity: annotation944790
display id: annotation944790
version: 1
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component: BBa_B0033

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BBa_K1861070
type: ComponentDefinition
persistent identity: BBa K1861070
display id: BBa_K1861070
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Fusion protein of TALE3 and a SpyTag in an expression cassette
creator: Vladyslav Vyshnevskyi
created: 2015-09-17T11:00:00Z
modified: 2015-09-23T03:37:16Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2291_
m user id: 25348
owner id: 25348
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Coming soon.
mutable notes: Coming soon.
mutable provenance: Coming soon.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1159201
component: BBa_K1861701
component: BBa_K392003
component: BBa_K1861101
component: BBa_K1861102
component: BBa_K1861104
sequence annotation: BBa_K1861701
sequence annotation: BBa_K1861102
sequence annotation: BBa_K1861104
sequence annotation: BBa_K1861101
sequence annotation: BBa_K392003
sequence annotation: BBa_K1159201
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BBa_K1159201
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display id: component2474673
version: 1
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access: public
definition: https://synbiohub.org/public/igem/BBa_K1159201/1

BBa_K1861701
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display id: component2474670
version: 1
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access: public
definition: https://synbiohub.org/public/igem/BBa_K1861701/1

BBa_K392003
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version: 1
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access: public
definition: https://synbiohub.org/public/igem/BBa_K392003/1

BBa_K1861101
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version: 1
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definition: https://synbiohub.org/public/igem/BBa_K1861101/1

BBa_K1861102
type: Component
persistent identity: component2474669
display id: component2474669
version: 1
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access: public
definition: https://synbiohub.org/public/igem/BBa_K1861102/1

BBa_K1861104
type: Component
persistent identity: component2474674
display id: component2474674
version: 1
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access: public
definition: https://synbiohub.org/public/igem/BBa_K1861104/1

BBa_K1861701
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persistent identity: annotation2474670
display id: annotation2474670
version: 1
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component: BBa_K1861701

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BBa_K1861102
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top level: BBa_K1861070
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component: BBa_K1861102

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persistent identity: range2474669
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version: 1
direction: direction/reverse
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end: 265
orientation: inline

BBa_K1861104
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location: https://synbiohub.org/public/igem/BBa_K1861070/annotation2474674/range2474674/1
component: BBa_K1861104

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BBa_K1861101
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version: 1
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BBa_K392003
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display id: annotation2474675
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component: BBa_K392003

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BBa_K1159201
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persistent identity: annotation2474673
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