//function/reporter/pigment
type: Collection
persistent identity: function reporter pigment
display id: function_reporter_pigment
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
owned by: james
owned by: myers
member: BBa_K274210
member: aeBlue
member: BBa_K1824017
member: BBa_K1838007
member: BBa_K1943003
member: meffBlue
member: eforRed
member: BBa_K1033932
member: BBa_K1838006
member: BBa_J04450
member: spisPink
member: amilCP
member: BBa_K1943007
member: BBa_K1033916
member: BBa_K1685007
member: BBa_K1943002
member: BBa_K1943006
member: BBa_K1943001
member: BBa_K1838005
member: BBa_K1122007
member: BBa_K1685008
member: BBa_K274100
member: BBa_K274200
member: amilGFP
member: BBa_K1023005
member: yA
member: gfasPurple
member: BBa_K1943000
member: BBa_K274110
member: tsPurple
member: BBa_K1824019
member: BBa_K1943005
member: cjBlue
member: fwYellow
member: BBa_K1943004
member: BBa_K1467201
member: BBa_K1685005

BBa_K1033932
type: ComponentDefinition
persistent identity: BBa K1033932
display id: BBa_K1033932
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: spisPink, pink chromoprotein
creator: Erik Lundin
created: 2014-04-20T11:00:00Z
modified: 2015-05-08T01:08:50Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1340_
m user id: 0
owner id: 9827
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This chromoprotein from the coral Stylophora pistillata, spisPink (also known as spisCP), naturally exhibits strong color when expressed. The protein has an absorption maximum at 560 nm giving it a pink color visible to the naked eye. The strong color is readily observed in both LB or on agar plates after less than 24 hours of incubation. The protein spisPink has significant sequence homologies with proteins in the GFP family.
mutable notes: Synthesized and codon optimized for E coli by GenScript.
mutable provenance: Stylophora pistillata. The protein was first extracted and characterized by Alieva et. al. under the name spisCP (GenBank: ABB17971.1). This version is codon optimized for E coli by Genscript.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: spisPink
sequence: https://synbiohub.org/public/igem/BBa_K1033932_sequence/1

spisPink
type: SequenceAnnotation
persistent identity: annotation2372666
display id: annotation2372666
version: 1
top level: BBa_K1033932
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1033932/annotation2372666/range2372666/1

https://synbiohub.org/public/igem/BBa_K1033932/annotation2372666/range2372666/1
type: Range
persistent identity: range2372666
display id: range2372666
version: 1
direction: direction/forward
top level: BBa_K1033932
start: 1
end: 678
orientation: inline

amilGFP
type: ComponentDefinition
persistent identity: BBa K592010
display id: BBa_K592010
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: amilGFP, yellow chromoprotein
creator: Lei Sun
created: 2011-09-17T11:00:00Z
modified: 2015-05-08T01:12:48Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _763_
m user id: 0
owner id: 7929
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This chromoprotein, amilGFP, naturally exhibits very strong yellow color when expressed. The color is strong and readily visible to naked eye both in LB-culture and on agar plates. The DNA was provided by Jeffrey Miller at UCLA. It was made BioBrick-compatible after removal of two illegal internal restriction sites (EcoRI and PstI).
mutable notes: Two illegal internal restriction sites (EcoRI and PstI) was removed.
mutable provenance: Acropora millepora
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: amilGFP
sequence: https://synbiohub.org/public/igem/BBa_K592010_sequence/1

amilGFP
type: SequenceAnnotation
persistent identity: annotation2131633
display id: annotation2131633
version: 1
top level: amilGFP
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K592010/annotation2131633/range2131633/1

https://synbiohub.org/public/igem/BBa_K592010/annotation2131633/range2131633/1
type: Range
persistent identity: range2131633
display id: range2131633
version: 1
direction: direction/forward
top level: amilGFP
start: 1
end: 696
orientation: inline

BBa_K1943001
type: ComponentDefinition
persistent identity: BBa K1943001
display id: BBa_K1943001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: fwYellow, yellow chromoprotein reporter system (medium Promoter , weak RBS)
creator: Shixin Lu, Shiqiang Tang
created: 2016-08-28T11:00:00Z
modified: 2016-10-21T08:02:33Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2410_
m user id: 30006
owner id: 29845
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Team Uppsala 2012 chromoprotein attracts many interests because it's more convenient than fluorescent protein for it can be observed with naked eyes. However, characterized data of these proteins are few. Because a single coding region is on the plasmid, we constructed BBa_K1943001 with chromoprotein fwYellow(BBa_K1033910) this year and did characterization. We've constructed a series of plasmids which are all in the same pattern: a strong/weak promoter, a strong/weak RBS and a chromoprotein. We want to monitor the speed of expression of these plasmids in normal incubation conditions (like 37℃ overnight for LB agar plate and 37℃ 180rpm for LB broth). The expression speed and strength of the constructed biobricks will be carefully monitored and gave others a relative scale for using chromoprotein as a reporter gene.
mutable notes: no
mutable provenance: basic parts
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_B0015
component: BBa_K1033910
component: BBa_K608007
sequence annotation: BBa_K1033910
sequence annotation: BBa_K608007
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_K1943001_sequence/1

BBa_B0015
type: Component
persistent identity: component2481880
display id: component2481880
version: 1
top level: BBa_K1943001
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_K1033910
type: Component
persistent identity: component2481873
display id: component2481873
version: 1
top level: BBa_K1943001
access: public
definition: fwYellow

BBa_K608007
type: Component
persistent identity: component2481871
display id: component2481871
version: 1
top level: BBa_K1943001
access: public
definition: https://synbiohub.org/public/igem/BBa_K608007/1

BBa_K1033910
type: SequenceAnnotation
persistent identity: annotation2481873
display id: annotation2481873
version: 1
top level: BBa_K1943001
location: https://synbiohub.org/public/igem/BBa_K1943001/annotation2481873/range2481873/1
component: BBa_K1033910

https://synbiohub.org/public/igem/BBa_K1943001/annotation2481873/range2481873/1
type: Range
persistent identity: range2481873
display id: range2481873
version: 1
direction: direction/reverse
top level: BBa_K1943001
start: 64
end: 777
orientation: inline

BBa_K608007
type: SequenceAnnotation
persistent identity: annotation2481871
display id: annotation2481871
version: 1
top level: BBa_K1943001
location: https://synbiohub.org/public/igem/BBa_K1943001/annotation2481871/range2481871/1
component: BBa_K608007

https://synbiohub.org/public/igem/BBa_K1943001/annotation2481871/range2481871/1
type: Range
persistent identity: range2481871
display id: range2481871
version: 1
direction: direction/reverse
top level: BBa_K1943001
start: 1
end: 57
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2481880
display id: annotation2481880
version: 1
top level: BBa_K1943001
location: https://synbiohub.org/public/igem/BBa_K1943001/annotation2481880/range2481880/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K1943001/annotation2481880/range2481880/1
type: Range
persistent identity: range2481880
display id: range2481880
version: 1
direction: direction/reverse
top level: BBa_K1943001
start: 786
end: 914
orientation: inline

BBa_K274200
type: ComponentDefinition
persistent identity: BBa K274200
display id: BBa_K274200
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CrtEBIY with rbs
creator: Siming Ma
created: 2009-09-17T11:00:00Z
modified: 2015-05-08T01:11:45Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _374_
m user id: 0
owner id: 5416
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This Composite Biobrick is created by standard assembly of Part BBa_K118014 (CrtE with rbs), Part BBa_K118006 (CrtB with rbs), Part BBa_K118005 (CrtI with rbs) and Part BBa_K118013 (CrtY with rbs), which are submitted by previous iGEM teams. The whole cassette is on plasmid pSB1A2 (high copy, Ampicillin resistance). Together, enzymes CrtE, CrtB, CrtI and CrtY convert colourless farnesyl pyrophosphate to orange beta-carotene (via intermediates geranylgeranyl pyroiphosphate, phytoene and lycopene). The orange beta-carotene pigment can then be used as a coloured signal output, e.g. for biosensors. There is already individual ribosome binding site before each enzyme gene sequence. Internal restriction sites have been removed by previous iGEM teams. Please refer to Parts BBa_K118014, BBa_K118006, BBa_K118005 and BBa_K118013 for more information on individual Biobricks components. This Composite Biobrick has been put under constitutive promoter R0011 (see Part BBa_K274210) and arabinose-inducible promoter I0500 (see Part BBa_274220), transformed and tested in E.coli strain MG1655. Amount of beta-carotene produced can be measured by photospectrometer with absorbance at 455nm (beta-carotene extraction using acetone). A related Composite Biobrick is Part BBa_K274100 (CrtEBI with rbs), which "stops" at one step before beta-carotene, producing (and retaining) red lycopene pigment.
mutable notes: The colourless precursor, farnesyl pyrophosphate, is naturally produced in E.coli using Non-mevalonate Pathway (from pyruvate and glyceraldehyde-3-phosphate). Our experiences showed that this Biobrick works better in E.coli strains with up-regulated level of precursors. E.coli strain MG1655 demonostrates higher level of pigment (beta-carotene) production than strain TOP10.
mutable provenance: Gene coding sequences come from Pantoea ananatis (formerly Erwinia uredovora) (Accession number D90087), a type of Gram negative Enterobacteriaceae. This Composite Biobrick is created by standard assembly of Part BBa_K118014, Part BBa_K118006, Part BBa_K118005 and Part BBa_K118013.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K118002
component: BBa_K118003
component: BBa_K118012
component: BBa_J15001
component: BBa_K118008
component: BBa_I742151
sequence annotation: BBa_J15001
sequence annotation: BBa_K118008
sequence annotation: BBa_K118012
sequence annotation: BBa_I742151
sequence annotation: BBa_K118003
sequence annotation: BBa_K118002
sequence: https://synbiohub.org/public/igem/BBa_K274200_sequence/1

BBa_K118002
type: Component
persistent identity: component2021586
display id: component2021586
version: 1
top level: BBa_K274200
access: public
definition: https://synbiohub.org/public/igem/BBa_K118002/1

BBa_K118003
type: Component
persistent identity: component2021593
display id: component2021593
version: 1
top level: BBa_K274200
access: public
definition: https://synbiohub.org/public/igem/BBa_K118003/1

BBa_K118012
type: Component
persistent identity: component2021594
display id: component2021594
version: 1
top level: BBa_K274200
access: public
definition: https://synbiohub.org/public/igem/BBa_K118012/1

BBa_J15001
type: Component
persistent identity: component2021589
display id: component2021589
version: 1
top level: BBa_K274200
access: public
definition: https://synbiohub.org/public/igem/BBa_J15001/1

BBa_J15001
type: Component
persistent identity: component2021584
display id: component2021584
version: 1
top level: BBa_K274200
access: public
definition: https://synbiohub.org/public/igem/BBa_J15001/1

BBa_K118012
type: Component
persistent identity: component2021580
display id: component2021580
version: 1
top level: BBa_K274200
access: public
definition: https://synbiohub.org/public/igem/BBa_K118012/1

BBa_K118008
type: Component
persistent identity: component2021596
display id: component2021596
version: 1
top level: BBa_K274200
access: public
definition: https://synbiohub.org/public/igem/BBa_K118008/1

BBa_I742151
type: Component
persistent identity: component2021581
display id: component2021581
version: 1
top level: BBa_K274200
access: public
definition: https://synbiohub.org/public/igem/BBa_I742151/1

BBa_J15001
type: SequenceAnnotation
persistent identity: annotation2021589
display id: annotation2021589
version: 1
top level: BBa_K274200
location: https://synbiohub.org/public/igem/BBa_K274200/annotation2021589/range2021589/1
component: BBa_J15001

https://synbiohub.org/public/igem/BBa_K274200/annotation2021589/range2021589/1
type: Range
persistent identity: range2021589
display id: range2021589
version: 1
direction: direction/reverse
top level: BBa_K274200
start: 1888
end: 1897
orientation: inline

BBa_K118008
type: SequenceAnnotation
persistent identity: annotation2021596
display id: annotation2021596
version: 1
top level: BBa_K274200
location: https://synbiohub.org/public/igem/BBa_K274200/annotation2021596/range2021596/1
component: BBa_K118008

https://synbiohub.org/public/igem/BBa_K274200/annotation2021596/range2021596/1
type: Range
persistent identity: range2021596
display id: range2021596
version: 1
direction: direction/reverse
top level: BBa_K274200
start: 3404
end: 4555
orientation: inline

BBa_K118012
type: SequenceAnnotation
persistent identity: annotation2021594
display id: annotation2021594
version: 1
top level: BBa_K274200
location: https://synbiohub.org/public/igem/BBa_K274200/annotation2021594/range2021594/1
component: BBa_K118012

https://synbiohub.org/public/igem/BBa_K274200/annotation2021594/range2021594/1
type: Range
persistent identity: range2021594
display id: range2021594
version: 1
direction: direction/reverse
top level: BBa_K274200
start: 3394
end: 3397
orientation: inline

BBa_I742151
type: SequenceAnnotation
persistent identity: annotation2021581
display id: annotation2021581
version: 1
top level: BBa_K274200
location: https://synbiohub.org/public/igem/BBa_K274200/annotation2021581/range2021581/1
component: BBa_I742151

https://synbiohub.org/public/igem/BBa_K274200/annotation2021581/range2021581/1
type: Range
persistent identity: range2021581
display id: range2021581
version: 1
direction: direction/reverse
top level: BBa_K274200
start: 11
end: 922
orientation: inline

BBa_K118012
type: SequenceAnnotation
persistent identity: annotation2021580
display id: annotation2021580
version: 1
top level: BBa_K274200
location: https://synbiohub.org/public/igem/BBa_K274200/annotation2021580/range2021580/1
component: BBa_K118012

https://synbiohub.org/public/igem/BBa_K274200/annotation2021580/range2021580/1
type: Range
persistent identity: range2021580
display id: range2021580
version: 1
direction: direction/reverse
top level: BBa_K274200
start: 1
end: 4
orientation: inline

BBa_K118003
type: SequenceAnnotation
persistent identity: annotation2021593
display id: annotation2021593
version: 1
top level: BBa_K274200
location: https://synbiohub.org/public/igem/BBa_K274200/annotation2021593/range2021593/1
component: BBa_K118003

https://synbiohub.org/public/igem/BBa_K274200/annotation2021593/range2021593/1
type: Range
persistent identity: range2021593
display id: range2021593
version: 1
direction: direction/reverse
top level: BBa_K274200
start: 1904
end: 3385
orientation: inline

BBa_J15001
type: SequenceAnnotation
persistent identity: annotation2021584
display id: annotation2021584
version: 1
top level: BBa_K274200
location: https://synbiohub.org/public/igem/BBa_K274200/annotation2021584/range2021584/1
component: BBa_J15001

https://synbiohub.org/public/igem/BBa_K274200/annotation2021584/range2021584/1
type: Range
persistent identity: range2021584
display id: range2021584
version: 1
direction: direction/reverse
top level: BBa_K274200
start: 931
end: 940
orientation: inline

BBa_K118002
type: SequenceAnnotation
persistent identity: annotation2021586
display id: annotation2021586
version: 1
top level: BBa_K274200
location: https://synbiohub.org/public/igem/BBa_K274200/annotation2021586/range2021586/1
component: BBa_K118002

https://synbiohub.org/public/igem/BBa_K274200/annotation2021586/range2021586/1
type: Range
persistent identity: range2021586
display id: range2021586
version: 1
direction: direction/reverse
top level: BBa_K274200
start: 947
end: 1879
orientation: inline

BBa_K1685005
type: ComponentDefinition
persistent identity: BBa K1685005
display id: BBa_K1685005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: aeBlue
creator: ITB_Indonesia 2015
created: 2015-09-15T11:00:00Z
modified: 2016-01-25T01:09:54Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2103_
m user id: 4206
owner id: 25100
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This part encodes a blue chromoprotein named aeBlue. This part is useful for reporter.
mutable notes: The part has been codon-optimised for expression in E. coli using http://sg.idtdna.com/CodonOpt
mutable provenance: The aeBlue sequence from Uppsala team 2012 (BBa_K864401) was chemically synthesised.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1685005_sequence/1

BBa_K1943006
type: ComponentDefinition
persistent identity: BBa K1943006
display id: BBa_K1943006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: spisPink, Pink chromoprotein reporter system (Strong Promoter, Strong RBS)
creator: Shixin Lu, Shiqiang Tang
created: 2016-08-28T11:00:00Z
modified: 2016-10-21T08:40:40Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2410_
m user id: 30006
owner id: 29845
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Team Uppsala 2012 chromoprotein attracts many interests because it's more convenient than fluorescent protein for it can be observed with naked eyes. However, characterized data of these proteins are few. Because a single coding region is on the plasmid, we constructed BBa_K1943006 with chromoprotein spisPink(BBa_K1033932) this year and did characterization. We've constructed a series of plasmids which are all in the same pattern: a strong/weak promoter, a strong/weak RBS and a chromoprotein. We want to monitor the speed of expression of these plasmids in normal incubation conditions (like 37℃ overnight for LB agar plate and 37℃ 180rpm for LB broth). The expression speed and strength of the constructed biobricks will be carefully monitored and gave others a relative scale for using chromoprotein as a reporter gene. For detailed characterization data, see the experience page.
mutable notes: no
mutable provenance: basic parts
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_B0015
component: BBa_K880005
component: BBa_K1033932
sequence annotation: BBa_K1033932
sequence annotation: BBa_K880005
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_K1943006_sequence/1

BBa_B0015
type: Component
persistent identity: component2512759
display id: component2512759
version: 1
top level: BBa_K1943006
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_K880005
type: Component
persistent identity: component2512750
display id: component2512750
version: 1
top level: BBa_K1943006
access: public
definition: https://synbiohub.org/public/igem/BBa_K880005/1

BBa_K1033932
type: Component
persistent identity: component2512752
display id: component2512752
version: 1
top level: BBa_K1943006
access: public
definition: BBa_K1033932

BBa_K1033932
type: SequenceAnnotation
persistent identity: annotation2512752
display id: annotation2512752
version: 1
top level: BBa_K1943006
location: https://synbiohub.org/public/igem/BBa_K1943006/annotation2512752/range2512752/1
component: BBa_K1033932

https://synbiohub.org/public/igem/BBa_K1943006/annotation2512752/range2512752/1
type: Range
persistent identity: range2512752
display id: range2512752
version: 1
direction: direction/reverse
top level: BBa_K1943006
start: 62
end: 739
orientation: inline

BBa_K880005
type: SequenceAnnotation
persistent identity: annotation2512750
display id: annotation2512750
version: 1
top level: BBa_K1943006
location: https://synbiohub.org/public/igem/BBa_K1943006/annotation2512750/range2512750/1
component: BBa_K880005

https://synbiohub.org/public/igem/BBa_K1943006/annotation2512750/range2512750/1
type: Range
persistent identity: range2512750
display id: range2512750
version: 1
direction: direction/reverse
top level: BBa_K1943006
start: 1
end: 55
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2512759
display id: annotation2512759
version: 1
top level: BBa_K1943006
location: https://synbiohub.org/public/igem/BBa_K1943006/annotation2512759/range2512759/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K1943006/annotation2512759/range2512759/1
type: Range
persistent identity: range2512759
display id: range2512759
version: 1
direction: direction/reverse
top level: BBa_K1943006
start: 748
end: 876
orientation: inline

BBa_K1467201
type: ComponentDefinition
persistent identity: BBa K1467201
display id: BBa_K1467201
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: GoldenGate compatible AmilCP, Blue chromoprotein
creator: Cara Deal
created: 2014-09-23T11:00:00Z
modified: 2015-06-01T07:04:22Z
discontinued: false
dominant: false
group u list: _1846_
m user id: 4206
owner id: 21550
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: A variation of the part BBa_K592009 that has been made compatible with the GoldenGate MoClo Assembly Standard as it is free from BsaI and BpiI recognition sequences. The chromoprotein naturally exhibits strong blue color when expressed. Codon-optimised for expression in dicotyledenous plants.
mutable notes: Domesticated for GoldenGate by removal of internal BsaI and BpiI recognition sequences. Codon-optimised for expression in dicotyledenous plants.
mutable provenance: Acropora millepora
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence: https://synbiohub.org/public/igem/BBa_K1467201_sequence/1

BBa_J04450
type: ComponentDefinition
persistent identity: BBa J04450
display id: BBa_J04450
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RFP Coding Device
creator: Tamar Odle
created: 2005-06-08T11:00:00Z
modified: 2015-08-31T04:08:14Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _16_
m user id: 0
owner id: 328
owning group id: 16
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Contains an IPTG inducible promoter an RBS, RFP, and terminator.
mutable provenance: Davidson Synth-Aces
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_B0034
component: BBa_E1010
component: BBa_B0012
component: BBa_B0010
component: BBa_R0010
sequence annotation: BBa_B0012
sequence annotation: BBa_E1010
sequence annotation: BBa_R0010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_J04450_sequence/1

BBa_B0034
type: Component
persistent identity: component1509404
display id: component1509404
version: 1
top level: BBa_J04450
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_E1010
type: Component
persistent identity: component1509411
display id: component1509411
version: 1
top level: BBa_J04450
access: public
definition: https://synbiohub.org/public/igem/BBa_E1010/1

BBa_B0012
type: Component
persistent identity: component1509427
display id: component1509427
version: 1
top level: BBa_J04450
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0010
type: Component
persistent identity: component1509417
display id: component1509417
version: 1
top level: BBa_J04450
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_R0010
type: Component
persistent identity: component1509394
display id: component1509394
version: 1
top level: BBa_J04450
access: public
definition: https://synbiohub.org/public/igem/BBa_R0010/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1509427
display id: annotation1509427
version: 1
top level: BBa_J04450
location: https://synbiohub.org/public/igem/BBa_J04450/annotation1509427/range1509427/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_J04450/annotation1509427/range1509427/1
type: Range
persistent identity: range1509427
display id: range1509427
version: 1
direction: direction/forward
top level: BBa_J04450
start: 1029
end: 1069
orientation: inline

BBa_E1010
type: SequenceAnnotation
persistent identity: annotation1509411
display id: annotation1509411
version: 1
top level: BBa_J04450
location: https://synbiohub.org/public/igem/BBa_J04450/annotation1509411/range1509411/1
component: BBa_E1010

https://synbiohub.org/public/igem/BBa_J04450/annotation1509411/range1509411/1
type: Range
persistent identity: range1509411
display id: range1509411
version: 1
direction: direction/forward
top level: BBa_J04450
start: 227
end: 907
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation1509394
display id: annotation1509394
version: 1
top level: BBa_J04450
location: https://synbiohub.org/public/igem/BBa_J04450/annotation1509394/range1509394/1
component: BBa_R0010

https://synbiohub.org/public/igem/BBa_J04450/annotation1509394/range1509394/1
type: Range
persistent identity: range1509394
display id: range1509394
version: 1
direction: direction/forward
top level: BBa_J04450
start: 1
end: 200
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1509417
display id: annotation1509417
version: 1
top level: BBa_J04450
location: https://synbiohub.org/public/igem/BBa_J04450/annotation1509417/range1509417/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_J04450/annotation1509417/range1509417/1
type: Range
persistent identity: range1509417
display id: range1509417
version: 1
direction: direction/forward
top level: BBa_J04450
start: 941
end: 1020
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1509404
display id: annotation1509404
version: 1
top level: BBa_J04450
location: https://synbiohub.org/public/igem/BBa_J04450/annotation1509404/range1509404/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_J04450/annotation1509404/range1509404/1
type: Range
persistent identity: range1509404
display id: range1509404
version: 1
direction: direction/forward
top level: BBa_J04450
start: 209
end: 220
orientation: inline

BBa_K1943003
type: ComponentDefinition
persistent identity: BBa K1943003
display id: BBa_K1943003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: gfasPurple, purple chromoprotein reporter system (medium Promoter , weak RBS)
creator: Shixin Lu, Shiqiang Tang
created: 2016-08-28T11:00:00Z
modified: 2016-10-21T08:34:54Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2410_
m user id: 30006
owner id: 29845
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Team Uppsala 2012 chromoprotein attracts many interests because it's more convenient than fluorescent protein for it can be observed with naked eyes. However, characterized data of these proteins are few. Because a single coding region is on the plasmid, we constructed BBa_K1943003 with chromoprotein gfasPurple(BBa_K1033919) this year and did characterization. We've constructed a series of plasmids which are all in the same pattern: a strong/weak promoter, a strong/weak RBS and a chromoprotein. We want to monitor the speed of expression of these plasmids in normal incubation conditions (like 37℃ overnight for LB agar plate and 37℃ 180rpm for LB broth). The expression speed and strength of the constructed biobricks will be carefully monitored and gave others a relative scale for using chromoprotein as a reporter gene. For detailed characterization data, see the experience page.
mutable notes: no
mutable provenance: basic parts
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_K608007
component: BBa_K1033919
component: BBa_B0015
sequence annotation: BBa_K1033919
sequence annotation: BBa_B0015
sequence annotation: BBa_K608007
sequence: https://synbiohub.org/public/igem/BBa_K1943003_sequence/1

BBa_K608007
type: Component
persistent identity: component2481846
display id: component2481846
version: 1
top level: BBa_K1943003
access: public
definition: https://synbiohub.org/public/igem/BBa_K608007/1

BBa_K1033919
type: Component
persistent identity: component2481848
display id: component2481848
version: 1
top level: BBa_K1943003
access: public
definition: gfasPurple

BBa_B0015
type: Component
persistent identity: component2481855
display id: component2481855
version: 1
top level: BBa_K1943003
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_K1033919
type: SequenceAnnotation
persistent identity: annotation2481848
display id: annotation2481848
version: 1
top level: BBa_K1943003
location: https://synbiohub.org/public/igem/BBa_K1943003/annotation2481848/range2481848/1
component: BBa_K1033919

https://synbiohub.org/public/igem/BBa_K1943003/annotation2481848/range2481848/1
type: Range
persistent identity: range2481848
display id: range2481848
version: 1
direction: direction/reverse
top level: BBa_K1943003
start: 64
end: 732
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2481855
display id: annotation2481855
version: 1
top level: BBa_K1943003
location: https://synbiohub.org/public/igem/BBa_K1943003/annotation2481855/range2481855/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K1943003/annotation2481855/range2481855/1
type: Range
persistent identity: range2481855
display id: range2481855
version: 1
direction: direction/reverse
top level: BBa_K1943003
start: 741
end: 869
orientation: inline

BBa_K608007
type: SequenceAnnotation
persistent identity: annotation2481846
display id: annotation2481846
version: 1
top level: BBa_K1943003
location: https://synbiohub.org/public/igem/BBa_K1943003/annotation2481846/range2481846/1
component: BBa_K608007

https://synbiohub.org/public/igem/BBa_K1943003/annotation2481846/range2481846/1
type: Range
persistent identity: range2481846
display id: range2481846
version: 1
direction: direction/reverse
top level: BBa_K1943003
start: 1
end: 57
orientation: inline

BBa_K1824017
type: ComponentDefinition
persistent identity: BBa K1824017
display id: BBa_K1824017
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: AeBlue blue chromoprotein
creator: Zhe Yang
created: 2015-09-13T11:00:00Z
modified: 2016-01-25T01:07:38Z
discontinued: false
dominant: false
group u list: _2250_
m user id: 4206
owner id: 24766
owning group id: 9
status: status/Planning
bookmark: false
mutable description: AeBlue chromoprotein could be expressed as a deep blue color in E.coli, which was added by Uppsala University in 2012.
mutable notes: No
mutable provenance: From Uppsala University in 2012.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: AeBlue chromoprotein
sequence: https://synbiohub.org/public/igem/BBa_K1824017_sequence/1

AeBlue chromoprotein
type: SequenceAnnotation
persistent identity: annotation2455427
display id: annotation2455427
version: 1
top level: BBa_K1824017
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1824017/annotation2455427/range2455427/1

https://synbiohub.org/public/igem/BBa_K1824017/annotation2455427/range2455427/1
type: Range
persistent identity: range2455427
display id: range2455427
version: 1
direction: direction/forward
top level: BBa_K1824017
start: 1
end: 699
orientation: inline

BBa_K1685007
type: ComponentDefinition
persistent identity: BBa K1685007
display id: BBa_K1685007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: aeBlue with LAA tag
creator: ITB_Indonesia 2015
created: 2015-09-15T11:00:00Z
modified: 2016-01-25T11:15:30Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2103_
m user id: 4206
owner id: 25100
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This part encodes a chromoprotein, aeBlue, with LAA degradation tag
mutable notes: The aeBlue sequence was codon-optimised using http://sg.idtdna.com/CodonOpt. The degradation tag is based on Edinburgh 2014.
mutable provenance: The BBa_K1685005 part is given LAA-degradation tag designed by Edinburgh 2014 team.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1685007_sequence/1

BBa_K1023005
type: ComponentDefinition
persistent identity: BBa K1023005
display id: BBa_K1023005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: eforRed
creator: Michiel Karrenbelt
created: 2013-09-16T11:00:00Z
modified: 2016-01-26T03:04:25Z
discontinued: false
dominant: false
group u list: _1330_
m user id: 4206
owner id: 15730
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: codon optimised chromoprotein
mutable notes: codon optimised
mutable provenance: Braunschweig via Uppsala
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1023005_sequence/1

fwYellow
type: ComponentDefinition
persistent identity: BBa K1033910
display id: BBa_K1033910
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: fwYellow, yellow chromoprotein
creator: Sabri Jamal
created: 2013-09-16T11:00:00Z
modified: 2016-01-25T02:41:53Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1340_
m user id: 4206
owner id: 13997
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This chromoprotein naturally exhibits strong color when expressed.
mutable notes: This construct is synthetic. It was synthesized from a template where the consensus sequence for the "color dependancy" in order to obtain a chromoprotein with a unique yellow color.
mutable provenance: Synthetic chromoprotein from DNA 2.0.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: fwYellow
sequence: https://synbiohub.org/public/igem/BBa_K1033910_sequence/1

fwYellow
type: SequenceAnnotation
persistent identity: annotation2371218
display id: annotation2371218
version: 1
top level: fwYellow
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1033910/annotation2371218/range2371218/1

https://synbiohub.org/public/igem/BBa_K1033910/annotation2371218/range2371218/1
type: Range
persistent identity: range2371218
display id: range2371218
version: 1
direction: direction/forward
top level: fwYellow
start: 1
end: 711
orientation: inline

tsPurple
type: ComponentDefinition
persistent identity: BBa K1033906
display id: BBa_K1033906
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: tsPurple, purple chromoprotein
creator: Sabri Jamal
created: 2013-08-24T11:00:00Z
modified: 2015-05-08T01:08:49Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1340_
m user id: 0
owner id: 10137
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This chromoprotein naturally exhibits strong color when expressed.
mutable notes: Cloned from TinselPurple vector, DNA 2.0.
mutable provenance: Synthetic chromoprotein from DNA 2.0.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: tsPurple
sequence: https://synbiohub.org/public/igem/BBa_K1033906_sequence/1

tsPurple
type: SequenceAnnotation
persistent identity: annotation2332100
display id: annotation2332100
version: 1
top level: tsPurple
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1033906/annotation2332100/range2332100/1

https://synbiohub.org/public/igem/BBa_K1033906/annotation2332100/range2332100/1
type: Range
persistent identity: range2332100
display id: range2332100
version: 1
direction: direction/forward
top level: tsPurple
start: 1
end: 690
orientation: inline

BBa_K1122007
type: ComponentDefinition
persistent identity: BBa K1122007
display id: BBa_K1122007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: AmilCP (BBa_K592009) compatible with Genabler assembly
creator: Lina Gasiūnaitė
created: 2013-08-12T11:00:00Z
modified: 2016-01-26T02:13:45Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1433_
m user id: 4206
owner id: 18510
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: AmilCP (BBa_K592009) part with inward facing EarI recognition sites at the ends. It is compatible with Genabler assembly.
mutable notes: To be compatible with Genabler assembly, the part has inward-facing EarI sites at the ends. When assembled by BioBrick assembly, five N-terminal residues MetAlaSerSerSer- and one C-terminal Ala residue will be added to the original sequence of BBa_K592009.
mutable provenance: AmilCP is based on the BioBrick BBa_K592009.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: stop
sequence annotation: AmilCP
sequence annotation: start
sequence annotation: EarI site
sequence: https://synbiohub.org/public/igem/BBa_K1122007_sequence/1

stop
type: SequenceAnnotation
persistent identity: annotation2346345
display id: annotation2346345
version: 1
top level: BBa_K1122007
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1122007/annotation2346345/range2346345/1

https://synbiohub.org/public/igem/BBa_K1122007/annotation2346345/range2346345/1
type: Range
persistent identity: range2346345
display id: range2346345
version: 1
direction: direction/forward
top level: BBa_K1122007
start: 679
end: 681
orientation: inline

AmilCP
type: SequenceAnnotation
persistent identity: annotation2346346
display id: annotation2346346
version: 1
top level: BBa_K1122007
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1122007/annotation2346346/range2346346/1

https://synbiohub.org/public/igem/BBa_K1122007/annotation2346346/range2346346/1
type: Range
persistent identity: range2346346
display id: range2346346
version: 1
direction: direction/forward
top level: BBa_K1122007
start: 1
end: 681
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2346343
display id: annotation2346343
version: 1
top level: BBa_K1122007
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1122007/annotation2346343/range2346343/1

https://synbiohub.org/public/igem/BBa_K1122007/annotation2346343/range2346343/1
type: Range
persistent identity: range2346343
display id: range2346343
version: 1
direction: direction/forward
top level: BBa_K1122007
start: 1
end: 3
orientation: inline

EarI site
type: SequenceAnnotation
persistent identity: annotation2346344
display id: annotation2346344
version: 1
top level: BBa_K1122007
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1122007/annotation2346344/range2346344/1

https://synbiohub.org/public/igem/BBa_K1122007/annotation2346344/range2346344/1
type: Range
persistent identity: range2346344
display id: range2346344
version: 1
direction: direction/forward
top level: BBa_K1122007
start: 6
end: 11
orientation: inline

EarI site
type: SequenceAnnotation
persistent identity: annotation2346347
display id: annotation2346347
version: 1
top level: BBa_K1122007
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1122007/annotation2346347/range2346347/1

https://synbiohub.org/public/igem/BBa_K1122007/annotation2346347/range2346347/1
type: Range
persistent identity: range2346347
display id: range2346347
version: 1
direction: direction/reverse
top level: BBa_K1122007
start: 680
end: 685
orientation: inline

BBa_K1838005
type: ComponentDefinition
persistent identity: BBa K1838005
display id: BBa_K1838005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: MLC Glucose Sensitive Yellow Chromoprotein Construct
creator: Connor McFadden
created: 2015-09-17T11:00:00Z
modified: 2016-01-21T02:20:44Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2264_
m user id: 4206
owner id: 26050
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This composite part features our MLC2 construct combined via 3A assembly with a yellow chromoprotein+RBS part (BBa_K1033931). During transcription, the MLC2 binding site is bound by the protein MLC, inhibiting production of yellow chromoprotein. As glucose concentration increases in the cell, more MLC is uptaken and bound to the membrane by a mechanism involving the transmembrane glucose permease called IICBGlu. This means that higher glucose concentrations subsequently result in higher expression levels of the yellow chromoprotein and thus makes it a glucose-inducible chromoprotein producing part. Specifically, this part tests the hypothesis for how MLC affects the inducibility of a strong promoter when placed downstream of the base promoter sequence.
mutable notes: One issue to consider was where to place the MLC binding site and what strength promoter to use as a base. This was solved by creating multiple variants of the promorer (MLC1-4). This part may be improved by considering how the RBS is affected by the position of the MLC binding site.
mutable provenance: 1. Plumbridge, Jacqueline. ???DNA Binding Sites for the MLC and NagC Proteins: Regulation of nagE, Encoding the N-Acetylglucosamine-Specific Transporter inEscherichia Coli.??? Nucleic Acids Research 29.2 (2001): 506???514. Print. 2. http://parts.igem.org/Part:BBa_J23110 3. http://parts.igem.org/Part:BBa_K1033931
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1033931
component: BBa_K1838001
sequence annotation: BBa_K1033931
sequence annotation: BBa_K1838001
sequence: https://synbiohub.org/public/igem/BBa_K1838005_sequence/1

BBa_K1033931
type: Component
persistent identity: component2473482
display id: component2473482
version: 1
top level: BBa_K1838005
access: public
definition: https://synbiohub.org/public/igem/BBa_K1033931/1

BBa_K1838001
type: Component
persistent identity: component2473477
display id: component2473477
version: 1
top level: BBa_K1838005
access: public
definition: https://synbiohub.org/public/igem/BBa_K1838001/1

BBa_K1033931
type: SequenceAnnotation
persistent identity: annotation2473482
display id: annotation2473482
version: 1
top level: BBa_K1838005
location: https://synbiohub.org/public/igem/BBa_K1838005/annotation2473482/range2473482/1
component: BBa_K1033931

https://synbiohub.org/public/igem/BBa_K1838005/annotation2473482/range2473482/1
type: Range
persistent identity: range2473482
display id: range2473482
version: 1
direction: direction/reverse
top level: BBa_K1838005
start: 67
end: 783
orientation: inline

BBa_K1838001
type: SequenceAnnotation
persistent identity: annotation2473477
display id: annotation2473477
version: 1
top level: BBa_K1838005
location: https://synbiohub.org/public/igem/BBa_K1838005/annotation2473477/range2473477/1
component: BBa_K1838001

https://synbiohub.org/public/igem/BBa_K1838005/annotation2473477/range2473477/1
type: Range
persistent identity: range2473477
display id: range2473477
version: 1
direction: direction/reverse
top level: BBa_K1838005
start: 1
end: 58
orientation: inline

gfasPurple
type: ComponentDefinition
persistent identity: BBa K1033919
display id: BBa_K1033919
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: gfasPurple, purple chromoprotein
creator: Sabri Jamal
created: 2013-08-24T11:00:00Z
modified: 2015-05-08T01:08:49Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1340_
m user id: 0
owner id: 10137
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This chromoprotein from the coral Galaxea fascicularis, gfasPurple, naturally exhibits strong color when expressed.
mutable notes: Synthesized and codon optimized for E coli by GenScript.
mutable provenance: The coral Galaxea fascicularis.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: gfasPurple
sequence: https://synbiohub.org/public/igem/BBa_K1033919_sequence/1

gfasPurple
type: SequenceAnnotation
persistent identity: annotation2332104
display id: annotation2332104
version: 1
top level: gfasPurple
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1033919/annotation2332104/range2332104/1

https://synbiohub.org/public/igem/BBa_K1033919/annotation2332104/range2332104/1
type: Range
persistent identity: range2332104
display id: range2332104
version: 1
direction: direction/forward
top level: gfasPurple
start: 1
end: 666
orientation: inline

aeBlue
type: ComponentDefinition
persistent identity: BBa K864401
display id: BBa_K864401
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: aeBlue blue chromoprotein
creator: Erik Lundin
created: 2012-09-24T11:00:00Z
modified: 2015-05-08T01:13:37Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1124_
m user id: 0
owner id: 9827
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: aeBlue
mutable notes: Synthesized and codon optimized for E. coli by Bioneer Corp.
mutable provenance: Source
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: aeBlue
sequence: https://synbiohub.org/public/igem/BBa_K864401_sequence/1

aeBlue
type: SequenceAnnotation
persistent identity: annotation2197206
display id: annotation2197206
version: 1
top level: aeBlue
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K864401/annotation2197206/range2197206/1

https://synbiohub.org/public/igem/BBa_K864401/annotation2197206/range2197206/1
type: Range
persistent identity: range2197206
display id: range2197206
version: 1
direction: direction/forward
top level: aeBlue
start: 1
end: 699
orientation: inline

meffBlue
type: ComponentDefinition
persistent identity: BBa K1033902
display id: BBa_K1033902
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: meffBlue, blue chromoprotein
creator: Erik Gullberg
created: 2013-08-24T11:00:00Z
modified: 2015-05-08T01:08:49Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1340_
m user id: 0
owner id: 10137
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This chromoprotein from the coral Montipora efflorescens, meffBlue, naturally exhibits strong color when expressed.
mutable notes: Synthesized and codon optimized for E coli by GenScript.
mutable provenance: The coral Montipora efflorescens.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: meffBlue
sequence: https://synbiohub.org/public/igem/BBa_K1033902_sequence/1

meffBlue
type: SequenceAnnotation
persistent identity: annotation2332099
display id: annotation2332099
version: 1
top level: meffBlue
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1033902/annotation2332099/range2332099/1

https://synbiohub.org/public/igem/BBa_K1033902/annotation2332099/range2332099/1
type: Range
persistent identity: range2332099
display id: range2332099
version: 1
direction: direction/forward
top level: meffBlue
start: 1
end: 669
orientation: inline

BBa_K1838006
type: ComponentDefinition
persistent identity: BBa K1838006
display id: BBa_K1838006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: MLC Glucose Sensitive Yellow Chromoprotein Construct
creator: Connor McFadden
created: 2015-09-17T11:00:00Z
modified: 2016-01-21T02:20:22Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2264_
m user id: 4206
owner id: 26050
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This composite part features our MLC2 construct combined via 3A assembly with a yellow chromoprotein+RBS part (BBa_K1033931). During transcription, the MLC2 binding site is bound by the protein MLC, inhibiting production of yellow chromoprotein. As glucose concentration increases in the cell, more MLC is uptaken and bound to the membrane by a mechanism involving the transmembrane glucose permease called IICBGlu. This means that higher glucose concentrations subsequently result in higher expression levels of the yellow chromoprotein and thus makes it a glucose-inducible chromoprotein producing part. Specifically, this part tests the hypothesis for how MLC affects the inducibility of an average strength promoter when placed upstream of the base promoter sequence.
mutable notes: One issue to consider was where to place the MLC binding site and what strength promoter to use as a base. This was solved by creating multiple variants of the promorer (MLC1-4). This part may be improved by considering how the RBS is affected by the position of the MLC binding site.
mutable provenance: 1. Plumbridge, Jacqueline. ???DNA Binding Sites for the MLC and NagC Proteins: Regulation of nagE, Encoding the N-Acetylglucosamine-Specific Transporter inEscherichia Coli.??? Nucleic Acids Research 29.2 (2001): 506???514. Print. 2. http://parts.igem.org/Part:BBa_J23110 3. http://parts.igem.org/Part:BBa_K1033931
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1838002
component: BBa_K1033931
sequence annotation: BBa_K1838002
sequence annotation: BBa_K1033931
sequence: https://synbiohub.org/public/igem/BBa_K1838006_sequence/1

BBa_K1838002
type: Component
persistent identity: component2473522
display id: component2473522
version: 1
top level: BBa_K1838006
access: public
definition: https://synbiohub.org/public/igem/BBa_K1838002/1

BBa_K1033931
type: Component
persistent identity: component2473527
display id: component2473527
version: 1
top level: BBa_K1838006
access: public
definition: https://synbiohub.org/public/igem/BBa_K1033931/1

BBa_K1838002
type: SequenceAnnotation
persistent identity: annotation2473522
display id: annotation2473522
version: 1
top level: BBa_K1838006
location: https://synbiohub.org/public/igem/BBa_K1838006/annotation2473522/range2473522/1
component: BBa_K1838002

https://synbiohub.org/public/igem/BBa_K1838006/annotation2473522/range2473522/1
type: Range
persistent identity: range2473522
display id: range2473522
version: 1
direction: direction/reverse
top level: BBa_K1838006
start: 1
end: 58
orientation: inline

BBa_K1033931
type: SequenceAnnotation
persistent identity: annotation2473527
display id: annotation2473527
version: 1
top level: BBa_K1838006
location: https://synbiohub.org/public/igem/BBa_K1838006/annotation2473527/range2473527/1
component: BBa_K1033931

https://synbiohub.org/public/igem/BBa_K1838006/annotation2473527/range2473527/1
type: Range
persistent identity: range2473527
display id: range2473527
version: 1
direction: direction/reverse
top level: BBa_K1838006
start: 67
end: 783
orientation: inline

BBa_K1943005
type: ComponentDefinition
persistent identity: BBa K1943005
display id: BBa_K1943005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: eforRed, Red chromoprotein reporter system (medium Promoter , weak RBS)
creator: Shixin Lu, Shiqiang Tang
created: 2016-08-28T11:00:00Z
modified: 2016-10-21T08:38:58Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2410_
m user id: 30006
owner id: 29845
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Team Uppsala 2012 chromoprotein attracts many interests because it's more convenient than fluorescent protein for it can be observed with naked eyes. However, characterized data of these proteins are few. Because a single coding region is on the plasmid, we constructed BBa_K1943005 with chromoprotein eforRed(BBa_K592012) this year and did characterization. We've constructed a series of plasmids which are all in the same pattern: a strong/weak promoter, a strong/weak RBS and a chromoprotein. We want to monitor the speed of expression of these plasmids in normal incubation conditions (like 37℃ overnight for LB agar plate and 37℃ 180rpm for LB broth). The expression speed and strength of the constructed biobricks will be carefully monitored and gave others a relative scale for using chromoprotein as a reporter gene. For detailed characterization data, see the experience page.
mutable notes: no
mutable provenance: basic parts
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_K592012
component: BBa_K608007
component: BBa_B0015
sequence annotation: BBa_B0015
sequence annotation: BBa_K608007
sequence annotation: BBa_K592012
sequence: https://synbiohub.org/public/igem/BBa_K1943005_sequence/1

BBa_K592012
type: Component
persistent identity: component2481910
display id: component2481910
version: 1
top level: BBa_K1943005
access: public
definition: eforRed

BBa_K608007
type: Component
persistent identity: component2481908
display id: component2481908
version: 1
top level: BBa_K1943005
access: public
definition: https://synbiohub.org/public/igem/BBa_K608007/1

BBa_B0015
type: Component
persistent identity: component2481917
display id: component2481917
version: 1
top level: BBa_K1943005
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2481917
display id: annotation2481917
version: 1
top level: BBa_K1943005
location: https://synbiohub.org/public/igem/BBa_K1943005/annotation2481917/range2481917/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K1943005/annotation2481917/range2481917/1
type: Range
persistent identity: range2481917
display id: range2481917
version: 1
direction: direction/reverse
top level: BBa_K1943005
start: 753
end: 881
orientation: inline

BBa_K608007
type: SequenceAnnotation
persistent identity: annotation2481908
display id: annotation2481908
version: 1
top level: BBa_K1943005
location: https://synbiohub.org/public/igem/BBa_K1943005/annotation2481908/range2481908/1
component: BBa_K608007

https://synbiohub.org/public/igem/BBa_K1943005/annotation2481908/range2481908/1
type: Range
persistent identity: range2481908
display id: range2481908
version: 1
direction: direction/reverse
top level: BBa_K1943005
start: 1
end: 57
orientation: inline

BBa_K592012
type: SequenceAnnotation
persistent identity: annotation2481910
display id: annotation2481910
version: 1
top level: BBa_K1943005
location: https://synbiohub.org/public/igem/BBa_K1943005/annotation2481910/range2481910/1
component: BBa_K592012

https://synbiohub.org/public/igem/BBa_K1943005/annotation2481910/range2481910/1
type: Range
persistent identity: range2481910
display id: range2481910
version: 1
direction: direction/reverse
top level: BBa_K1943005
start: 64
end: 744
orientation: inline

spisPink
type: ComponentDefinition
persistent identity: BBa K1033925
display id: BBa_K1033925
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: spisPink, pink chromoprotein (incl RBS)
creator: Erik Lundin
created: 2013-09-16T11:00:00Z
modified: 2015-05-08T01:08:50Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1340_
m user id: 0
owner id: 13997
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This chromoprotein from the coral Stylophora pistillata, spisPink, naturally exhibits color when expressed.
mutable notes: Synthesized and codon optimized for E coli by GenScript.
mutable provenance: Stylophora pistillata
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: spisPink
sequence annotation: rbs
sequence: https://synbiohub.org/public/igem/BBa_K1033925_sequence/1

spisPink
type: SequenceAnnotation
persistent identity: annotation2351831
display id: annotation2351831
version: 1
top level: spisPink
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1033925/annotation2351831/range2351831/1

https://synbiohub.org/public/igem/BBa_K1033925/annotation2351831/range2351831/1
type: Range
persistent identity: range2351831
display id: range2351831
version: 1
direction: direction/forward
top level: spisPink
start: 20
end: 694
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation2351830
display id: annotation2351830
version: 1
top level: spisPink
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1033925/annotation2351830/range2351830/1

https://synbiohub.org/public/igem/BBa_K1033925/annotation2351830/range2351830/1
type: Range
persistent identity: range2351830
display id: range2351830
version: 1
direction: direction/forward
top level: spisPink
start: 5
end: 13
orientation: inline

cjBlue
type: ComponentDefinition
persistent identity: BBa K592011
display id: BBa_K592011
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: cjBlue, green chromoprotein
creator: Antonio Ascue Avalos
created: 2011-09-17T11:00:00Z
modified: 2015-05-08T01:12:49Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _763_
m user id: 0
owner id: 7929
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This chromoprotein, cjBlue, naturally exhibits strong green/blue color when expressed. Compared to amilCP(BBa_K592009) and amilGFP(BBa_K592010), the color development is slower. On agar plates and in liquid culture, the color is readily visible to naked eye after 24-48 hours of incubation. The DNA was codon-optimized for expression in E.coli and synthesized by the Korean company Bioneer Corporation.
mutable notes: Codon-optimization for expression in E.coli necessary.
mutable provenance: Cnidopus japonicus
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: cjBlue
sequence: https://synbiohub.org/public/igem/BBa_K592011_sequence/1

cjBlue
type: SequenceAnnotation
persistent identity: annotation2131787
display id: annotation2131787
version: 1
top level: cjBlue
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K592011/annotation2131787/range2131787/1

https://synbiohub.org/public/igem/BBa_K592011/annotation2131787/range2131787/1
type: Range
persistent identity: range2131787
display id: range2131787
version: 1
direction: direction/forward
top level: cjBlue
start: 1
end: 699
orientation: inline

BBa_K1943000
type: ComponentDefinition
persistent identity: BBa K1943000
display id: BBa_K1943000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: fwYellow, yellow chromoprotein reporter system (Strong Promoter, Strong RBS)
creator: Shixin Lu, Shiqiang Tang
created: 2016-08-28T11:00:00Z
modified: 2016-10-21T08:00:08Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2410_
m user id: 30006
owner id: 29845
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Team Uppsala 2012 chromoprotein attracts many interests because it's more convenient than fluorescent protein for it can be observed with naked eyes. However, characterized data of these proteins are few. Because a single coding region is on the plasmid, we constructed BBa_K1943000 with chromoprotein fwYellow(BBa_K1033910) this year and did characterization. We've constructed a series of plasmids which are all in the same pattern: a strong/weak promoter, a strong/weak RBS and a chromoprotein. We want to monitor the speed of expression of these plasmids in normal incubation conditions (like 37℃ overnight for LB agar plate and 37℃ 180rpm for LB broth). The expression speed and strength of the constructed biobricks will be carefully monitored and gave others a relative scale for using chromoprotein as a reporter gene.
mutable notes: 11
mutable provenance: basic parts
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_K880005
component: BBa_B0015
component: BBa_K1033910
sequence annotation: BBa_K880005
sequence annotation: BBa_B0015
sequence annotation: BBa_K1033910
sequence: https://synbiohub.org/public/igem/BBa_K1943000_sequence/1

BBa_K880005
type: Component
persistent identity: component2512711
display id: component2512711
version: 1
top level: BBa_K1943000
access: public
definition: https://synbiohub.org/public/igem/BBa_K880005/1

BBa_B0015
type: Component
persistent identity: component2512720
display id: component2512720
version: 1
top level: BBa_K1943000
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_K1033910
type: Component
persistent identity: component2512713
display id: component2512713
version: 1
top level: BBa_K1943000
access: public
definition: fwYellow

BBa_K880005
type: SequenceAnnotation
persistent identity: annotation2512711
display id: annotation2512711
version: 1
top level: BBa_K1943000
location: https://synbiohub.org/public/igem/BBa_K1943000/annotation2512711/range2512711/1
component: BBa_K880005

https://synbiohub.org/public/igem/BBa_K1943000/annotation2512711/range2512711/1
type: Range
persistent identity: range2512711
display id: range2512711
version: 1
direction: direction/reverse
top level: BBa_K1943000
start: 1
end: 55
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2512720
display id: annotation2512720
version: 1
top level: BBa_K1943000
location: https://synbiohub.org/public/igem/BBa_K1943000/annotation2512720/range2512720/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K1943000/annotation2512720/range2512720/1
type: Range
persistent identity: range2512720
display id: range2512720
version: 1
direction: direction/reverse
top level: BBa_K1943000
start: 784
end: 912
orientation: inline

BBa_K1033910
type: SequenceAnnotation
persistent identity: annotation2512713
display id: annotation2512713
version: 1
top level: BBa_K1943000
location: https://synbiohub.org/public/igem/BBa_K1943000/annotation2512713/range2512713/1
component: BBa_K1033910

https://synbiohub.org/public/igem/BBa_K1943000/annotation2512713/range2512713/1
type: Range
persistent identity: range2512713
display id: range2512713
version: 1
direction: direction/reverse
top level: BBa_K1943000
start: 62
end: 775
orientation: inline

BBa_K274100
type: ComponentDefinition
persistent identity: BBa K274100
display id: BBa_K274100
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CrtEBI with rbs
creator: Siming Ma
created: 2009-09-17T11:00:00Z
modified: 2015-05-08T01:11:45Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _374_
m user id: 0
owner id: 5416
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This Composite Biobrick is created by standard assembly of Part BBa_K118014 (CrtE with rbs), Part BBa_K118006 (CrtB with rbs) and Part BBa_K118005 (CrtI with rbs), which are submitted by previous iGEM teams. The whole cassette is on plasmid pSB1A2 (high copy, Ampicillin resistance). Together, enzymes CrtE, CrtB and CrtI convert colourless farnesyl pyrophosphate to red lycopene (via intermediates geranylgeranyl pyroiphosphate and phytoene). The red lycopene pigment can then be used as a coloured signal output, e.g. for biosensors. There is already individual ribosome binding site before each enzyme gene sequence. Internal restriction sites have been removed by previous iGEM teams. Please refer to Parts BBa_K118014, BBa_K118006 and BBa_K118005 for more information on individual Biobricks components. This Composite Biobrick has been put under constitutive promoter R0011 (see Part BBa_K274110) and arabinose-inducible promoter I0500 (see Part BBa_274120), transformed and tested in E.coli strain MG1655. Amount of lycopene produced can be measured by photospectrometer with absorbance at 475nm (lycopene extraction using acetone).
mutable notes: The colourless precursor, farnesyl pyrophosphate, is naturally produced in E.coli using Non-mevalonate Pathway (from pyruvate and glyceraldehyde-3-phosphate). Our experiences showed that the Composite Biobrick, when put under a promoter, works better in E.coli strains with up-regulated level of precursors. E.coli strain MG1655 demonostrates higher level of pigment (lycopene) production than strain TOP10.
mutable provenance: Gene coding sequences come from Pantoea ananatis (formerly Erwinia uredovora) (Accession number D90087), a type of Gram negative Enterobacteriaceae. This Composite Biobrick is created by standard assembly of Part BBa_K118014, Part BBa_K118006 and Part BBa_K118005.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K118002
component: BBa_I742151
component: BBa_K118003
component: BBa_J15001
component: BBa_K118012
sequence annotation: BBa_J15001
sequence annotation: BBa_I742151
sequence annotation: BBa_K118003
sequence annotation: BBa_K118002
sequence annotation: BBa_K118012
sequence: https://synbiohub.org/public/igem/BBa_K274100_sequence/1

BBa_K118002
type: Component
persistent identity: component2021354
display id: component2021354
version: 1
top level: BBa_K274100
access: public
definition: https://synbiohub.org/public/igem/BBa_K118002/1

BBa_I742151
type: Component
persistent identity: component2021349
display id: component2021349
version: 1
top level: BBa_K274100
access: public
definition: https://synbiohub.org/public/igem/BBa_I742151/1

BBa_K118003
type: Component
persistent identity: component2021361
display id: component2021361
version: 1
top level: BBa_K274100
access: public
definition: https://synbiohub.org/public/igem/BBa_K118003/1

BBa_J15001
type: Component
persistent identity: component2021357
display id: component2021357
version: 1
top level: BBa_K274100
access: public
definition: https://synbiohub.org/public/igem/BBa_J15001/1

BBa_J15001
type: Component
persistent identity: component2021352
display id: component2021352
version: 1
top level: BBa_K274100
access: public
definition: https://synbiohub.org/public/igem/BBa_J15001/1

BBa_K118012
type: Component
persistent identity: component2021348
display id: component2021348
version: 1
top level: BBa_K274100
access: public
definition: https://synbiohub.org/public/igem/BBa_K118012/1

BBa_J15001
type: SequenceAnnotation
persistent identity: annotation2021357
display id: annotation2021357
version: 1
top level: BBa_K274100
location: https://synbiohub.org/public/igem/BBa_K274100/annotation2021357/range2021357/1
component: BBa_J15001

https://synbiohub.org/public/igem/BBa_K274100/annotation2021357/range2021357/1
type: Range
persistent identity: range2021357
display id: range2021357
version: 1
direction: direction/reverse
top level: BBa_K274100
start: 1888
end: 1897
orientation: inline

BBa_J15001
type: SequenceAnnotation
persistent identity: annotation2021352
display id: annotation2021352
version: 1
top level: BBa_K274100
location: https://synbiohub.org/public/igem/BBa_K274100/annotation2021352/range2021352/1
component: BBa_J15001

https://synbiohub.org/public/igem/BBa_K274100/annotation2021352/range2021352/1
type: Range
persistent identity: range2021352
display id: range2021352
version: 1
direction: direction/reverse
top level: BBa_K274100
start: 931
end: 940
orientation: inline

BBa_I742151
type: SequenceAnnotation
persistent identity: annotation2021349
display id: annotation2021349
version: 1
top level: BBa_K274100
location: https://synbiohub.org/public/igem/BBa_K274100/annotation2021349/range2021349/1
component: BBa_I742151

https://synbiohub.org/public/igem/BBa_K274100/annotation2021349/range2021349/1
type: Range
persistent identity: range2021349
display id: range2021349
version: 1
direction: direction/reverse
top level: BBa_K274100
start: 11
end: 922
orientation: inline

BBa_K118003
type: SequenceAnnotation
persistent identity: annotation2021361
display id: annotation2021361
version: 1
top level: BBa_K274100
location: https://synbiohub.org/public/igem/BBa_K274100/annotation2021361/range2021361/1
component: BBa_K118003

https://synbiohub.org/public/igem/BBa_K274100/annotation2021361/range2021361/1
type: Range
persistent identity: range2021361
display id: range2021361
version: 1
direction: direction/reverse
top level: BBa_K274100
start: 1904
end: 3385
orientation: inline

BBa_K118002
type: SequenceAnnotation
persistent identity: annotation2021354
display id: annotation2021354
version: 1
top level: BBa_K274100
location: https://synbiohub.org/public/igem/BBa_K274100/annotation2021354/range2021354/1
component: BBa_K118002

https://synbiohub.org/public/igem/BBa_K274100/annotation2021354/range2021354/1
type: Range
persistent identity: range2021354
display id: range2021354
version: 1
direction: direction/reverse
top level: BBa_K274100
start: 947
end: 1879
orientation: inline

BBa_K118012
type: SequenceAnnotation
persistent identity: annotation2021348
display id: annotation2021348
version: 1
top level: BBa_K274100
location: https://synbiohub.org/public/igem/BBa_K274100/annotation2021348/range2021348/1
component: BBa_K118012

https://synbiohub.org/public/igem/BBa_K274100/annotation2021348/range2021348/1
type: Range
persistent identity: range2021348
display id: range2021348
version: 1
direction: direction/reverse
top level: BBa_K274100
start: 1
end: 4
orientation: inline

BBa_K1824019
type: ComponentDefinition
persistent identity: BBa K1824019
display id: BBa_K1824019
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: AeBlue blue chromoprotein with aav tag
creator: Zhe Yang
created: 2015-09-13T11:00:00Z
modified: 2016-01-25T01:08:09Z
discontinued: false
dominant: false
group u list: _2250_
m user id: 4206
owner id: 24766
owning group id: 9
status: status/Planning
bookmark: false
mutable description: AeBlue chromoprotein could be expressed as a deep blue color in E.coli, which was added by Uppsala University in 2012. We add lva tag to before stop codon to increase the degradation of the Aeblue chromoprotein, which is necessary in our earth map for color changing.
mutable notes: No
mutable provenance: Chromoprotein is extracted from Uppsala University in 2012.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Aeblue chromoprotein
sequence annotation: aav tag
sequence: https://synbiohub.org/public/igem/BBa_K1824019_sequence/1

Aeblue chromoprotein
type: SequenceAnnotation
persistent identity: annotation2455430
display id: annotation2455430
version: 1
top level: BBa_K1824019
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1824019/annotation2455430/range2455430/1

https://synbiohub.org/public/igem/BBa_K1824019/annotation2455430/range2455430/1
type: Range
persistent identity: range2455430
display id: range2455430
version: 1
direction: direction/forward
top level: BBa_K1824019
start: 1
end: 693
orientation: inline

aav tag
type: SequenceAnnotation
persistent identity: annotation2455431
display id: annotation2455431
version: 1
top level: BBa_K1824019
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1824019/annotation2455431/range2455431/1

https://synbiohub.org/public/igem/BBa_K1824019/annotation2455431/range2455431/1
type: Range
persistent identity: range2455431
display id: range2455431
version: 1
direction: direction/forward
top level: BBa_K1824019
start: 700
end: 735
orientation: inline

BBa_K274210
type: ComponentDefinition
persistent identity: BBa K274210
display id: BBa_K274210
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CrtEBIY under constitutive promoter
creator: Siming Ma
created: 2009-09-18T11:00:00Z
modified: 2015-05-08T01:11:45Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _374_
m user id: 0
owner id: 5416
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This Biobrick is created by putting enzyme cassette CrtEBIY (with individual rbs) of Part BBa_K274200 under constitutive promoter R0011. Enzyme cassette CrtEBIY (with individual rbs) of Part BBa_K274200 converts colourless farnesyl pyrophosphate to orange beta-carotene (via intermediates geranylgeranyl pyroiphosphate, phytoene and lycopene). Amount of beta-carotene produced can be measured by photospectrometer with absorbance at 455nm (beta-carotene extraction using acetone). Sequence and Features
mutable notes: The colourless precursor, farnesyl pyrophosphate, is naturally produced in E.coli using Non-mevalonate Pathway (from pyruvate and glyceraldehyde-3-phosphate). Our experiences showed that this Biobrick works better in E.coli strains with up-regulated level of precursors. E.coli strain MG1655 demonostrates higher level of pigment (lycopene) production than strain TOP10.
mutable provenance: Gene coding sequences come from Pantoea ananatis (formerly Erwinia uredovora) (Accession number D90087), a type of Gram negative Enterobacteriaceae. Please refer to Part BBa_K274200 for more information on individual components.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K118003
component: BBa_K118012
component: BBa_K118002
component: BBa_I742151
component: BBa_J15001
component: BBa_K118008
component: BBa_R0011
sequence annotation: BBa_K118012
sequence annotation: BBa_R0011
sequence annotation: BBa_I742151
sequence annotation: BBa_J15001
sequence annotation: BBa_K118008
sequence annotation: BBa_K118003
sequence annotation: BBa_K118002
sequence: https://synbiohub.org/public/igem/BBa_K274210_sequence/1

BBa_K118003
type: Component
persistent identity: component2021576
display id: component2021576
version: 1
top level: BBa_K274210
access: public
definition: https://synbiohub.org/public/igem/BBa_K118003/1

BBa_K118012
type: Component
persistent identity: component2021577
display id: component2021577
version: 1
top level: BBa_K274210
access: public
definition: https://synbiohub.org/public/igem/BBa_K118012/1

BBa_K118002
type: Component
persistent identity: component2021569
display id: component2021569
version: 1
top level: BBa_K274210
access: public
definition: https://synbiohub.org/public/igem/BBa_K118002/1

BBa_I742151
type: Component
persistent identity: component2021564
display id: component2021564
version: 1
top level: BBa_K274210
access: public
definition: https://synbiohub.org/public/igem/BBa_I742151/1

BBa_K118012
type: Component
persistent identity: component2021563
display id: component2021563
version: 1
top level: BBa_K274210
access: public
definition: https://synbiohub.org/public/igem/BBa_K118012/1

BBa_J15001
type: Component
persistent identity: component2021567
display id: component2021567
version: 1
top level: BBa_K274210
access: public
definition: https://synbiohub.org/public/igem/BBa_J15001/1

BBa_K118008
type: Component
persistent identity: component2021579
display id: component2021579
version: 1
top level: BBa_K274210
access: public
definition: https://synbiohub.org/public/igem/BBa_K118008/1

BBa_J15001
type: Component
persistent identity: component2021572
display id: component2021572
version: 1
top level: BBa_K274210
access: public
definition: https://synbiohub.org/public/igem/BBa_J15001/1

BBa_R0011
type: Component
persistent identity: component2021558
display id: component2021558
version: 1
top level: BBa_K274210
access: public
definition: https://synbiohub.org/public/igem/BBa_R0011/1

BBa_K118012
type: SequenceAnnotation
persistent identity: annotation2021577
display id: annotation2021577
version: 1
top level: BBa_K274210
location: https://synbiohub.org/public/igem/BBa_K274210/annotation2021577/range2021577/1
component: BBa_K118012

https://synbiohub.org/public/igem/BBa_K274210/annotation2021577/range2021577/1
type: Range
persistent identity: range2021577
display id: range2021577
version: 1
direction: direction/reverse
top level: BBa_K274210
start: 3457
end: 3460
orientation: inline

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation2021558
display id: annotation2021558
version: 1
top level: BBa_K274210
location: https://synbiohub.org/public/igem/BBa_K274210/annotation2021558/range2021558/1
component: BBa_R0011

https://synbiohub.org/public/igem/BBa_K274210/annotation2021558/range2021558/1
type: Range
persistent identity: range2021558
display id: range2021558
version: 1
direction: direction/forward
top level: BBa_K274210
start: 1
end: 54
orientation: inline

BBa_I742151
type: SequenceAnnotation
persistent identity: annotation2021564
display id: annotation2021564
version: 1
top level: BBa_K274210
location: https://synbiohub.org/public/igem/BBa_K274210/annotation2021564/range2021564/1
component: BBa_I742151

https://synbiohub.org/public/igem/BBa_K274210/annotation2021564/range2021564/1
type: Range
persistent identity: range2021564
display id: range2021564
version: 1
direction: direction/reverse
top level: BBa_K274210
start: 74
end: 985
orientation: inline

BBa_J15001
type: SequenceAnnotation
persistent identity: annotation2021572
display id: annotation2021572
version: 1
top level: BBa_K274210
location: https://synbiohub.org/public/igem/BBa_K274210/annotation2021572/range2021572/1
component: BBa_J15001

https://synbiohub.org/public/igem/BBa_K274210/annotation2021572/range2021572/1
type: Range
persistent identity: range2021572
display id: range2021572
version: 1
direction: direction/reverse
top level: BBa_K274210
start: 1951
end: 1960
orientation: inline

BBa_K118008
type: SequenceAnnotation
persistent identity: annotation2021579
display id: annotation2021579
version: 1
top level: BBa_K274210
location: https://synbiohub.org/public/igem/BBa_K274210/annotation2021579/range2021579/1
component: BBa_K118008

https://synbiohub.org/public/igem/BBa_K274210/annotation2021579/range2021579/1
type: Range
persistent identity: range2021579
display id: range2021579
version: 1
direction: direction/reverse
top level: BBa_K274210
start: 3467
end: 4618
orientation: inline

BBa_J15001
type: SequenceAnnotation
persistent identity: annotation2021567
display id: annotation2021567
version: 1
top level: BBa_K274210
location: https://synbiohub.org/public/igem/BBa_K274210/annotation2021567/range2021567/1
component: BBa_J15001

https://synbiohub.org/public/igem/BBa_K274210/annotation2021567/range2021567/1
type: Range
persistent identity: range2021567
display id: range2021567
version: 1
direction: direction/reverse
top level: BBa_K274210
start: 994
end: 1003
orientation: inline

BBa_K118003
type: SequenceAnnotation
persistent identity: annotation2021576
display id: annotation2021576
version: 1
top level: BBa_K274210
location: https://synbiohub.org/public/igem/BBa_K274210/annotation2021576/range2021576/1
component: BBa_K118003

https://synbiohub.org/public/igem/BBa_K274210/annotation2021576/range2021576/1
type: Range
persistent identity: range2021576
display id: range2021576
version: 1
direction: direction/reverse
top level: BBa_K274210
start: 1967
end: 3448
orientation: inline

BBa_K118002
type: SequenceAnnotation
persistent identity: annotation2021569
display id: annotation2021569
version: 1
top level: BBa_K274210
location: https://synbiohub.org/public/igem/BBa_K274210/annotation2021569/range2021569/1
component: BBa_K118002

https://synbiohub.org/public/igem/BBa_K274210/annotation2021569/range2021569/1
type: Range
persistent identity: range2021569
display id: range2021569
version: 1
direction: direction/reverse
top level: BBa_K274210
start: 1010
end: 1942
orientation: inline

BBa_K118012
type: SequenceAnnotation
persistent identity: annotation2021563
display id: annotation2021563
version: 1
top level: BBa_K274210
location: https://synbiohub.org/public/igem/BBa_K274210/annotation2021563/range2021563/1
component: BBa_K118012

https://synbiohub.org/public/igem/BBa_K274210/annotation2021563/range2021563/1
type: Range
persistent identity: range2021563
display id: range2021563
version: 1
direction: direction/reverse
top level: BBa_K274210
start: 64
end: 67
orientation: inline

eforRed
type: ComponentDefinition
persistent identity: BBa K592012
display id: BBa_K592012
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: eforRed, red chromoprotein
creator: Lei Sun
created: 2011-09-17T11:00:00Z
modified: 2015-05-08T01:12:49Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _763_
m user id: 0
owner id: 7929
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This chromoprotein eforRed naturally exhibits red color when expressed. The color is weaker than RFP, however. On agar plates and in liquid culture, the color is readily visible to naked eye in less than 24 hours of incubation. The DNA was codon-optimized for expression in E.coli and synthesized by the Korean company Bioneer Corporation.
mutable notes: coming soon
mutable provenance: coming soon
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: eforRed
sequence: https://synbiohub.org/public/igem/BBa_K592012_sequence/1

eforRed
type: SequenceAnnotation
persistent identity: annotation2131799
display id: annotation2131799
version: 1
top level: eforRed
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K592012/annotation2131799/range2131799/1

https://synbiohub.org/public/igem/BBa_K592012/annotation2131799/range2131799/1
type: Range
persistent identity: range2131799
display id: range2131799
version: 1
direction: direction/forward
top level: eforRed
start: 1
end: 681
orientation: inline

tsPurple
type: ComponentDefinition
persistent identity: BBa K1033905
display id: BBa_K1033905
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: tsPurple, purple chromoprotein (incl RBS)
creator: Erik Gullberg
created: 2013-09-16T11:00:00Z
modified: 2015-05-08T01:08:49Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1340_
m user id: 0
owner id: 13997
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This chromoprotein naturally exhibits strong color when expressed.
mutable notes: Cloned from TinselPurple vector, DNA 2.0.
mutable provenance: Synthetic chromoprotein from DNA 2.0.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: rbs
sequence annotation: tsPurple
sequence annotation: BBa_K1033906
sequence: https://synbiohub.org/public/igem/BBa_K1033905_sequence/1

rbs
type: SequenceAnnotation
persistent identity: annotation2349945
display id: annotation2349945
version: 1
top level: tsPurple
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1033905/annotation2349945/range2349945/1

https://synbiohub.org/public/igem/BBa_K1033905/annotation2349945/range2349945/1
type: Range
persistent identity: range2349945
display id: range2349945
version: 1
direction: direction/forward
top level: tsPurple
start: 1
end: 12
orientation: inline

tsPurple
type: SequenceAnnotation
persistent identity: annotation2349943
display id: annotation2349943
version: 1
top level: tsPurple
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1033905/annotation2349943/range2349943/1

https://synbiohub.org/public/igem/BBa_K1033905/annotation2349943/range2349943/1
type: Range
persistent identity: range2349943
display id: range2349943
version: 1
direction: direction/forward
top level: tsPurple
start: 19
end: 705
orientation: inline

BBa_K1033906
type: SequenceAnnotation
persistent identity: annotation2349944
display id: annotation2349944
version: 1
top level: tsPurple
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1033905/annotation2349944/range2349944/1

https://synbiohub.org/public/igem/BBa_K1033905/annotation2349944/range2349944/1
type: Range
persistent identity: range2349944
display id: range2349944
version: 1
direction: direction/reverse
top level: tsPurple
start: 19
end: 708
orientation: inline

BBa_K274110
type: ComponentDefinition
persistent identity: BBa K274110
display id: BBa_K274110
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CrtEBI under constitutive promoter
creator: Siming Ma
created: 2009-09-17T11:00:00Z
modified: 2015-05-08T01:11:45Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _374_
m user id: 0
owner id: 5416
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This Biobrick is created by putting enzyme cassette CrtEBI (with individual rbs) of Part BBa_K274100 under constitutive promoter R0011. Enzyme cassette CrtEBI (with individual rbs) of Part BBa_K274110 converts colourless farnesyl pyrophosphate to red lycopene (via intermediates geranylgeranyl pyroiphosphate and phytoene). Amount of lycopene produced can be measured by photospectrometer with absorbance at 475nm (lycopene extraction using acetone).
mutable notes: The colourless precursor, farnesyl pyrophosphate, is naturally produced in E.coli using Non-mevalonate Pathway (from pyruvate and glyceraldehyde-3-phosphate). Our experiences showed that this Biobrick works better in E.coli strains with up-regulated level of precursors. E.coli strain MG1655 demonostrates higher level of pigment (lycopene) production than strain TOP10.
mutable provenance: Gene coding sequences come from Pantoea ananatis (formerly Erwinia uredovora) (Accession number D90087), a type of Gram negative Enterobacteriaceae. Please refer to Part BBa_K274100 for more information on individual components.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_I742151
component: BBa_K118003
component: BBa_K118012
component: BBa_R0011
component: BBa_K118002
component: BBa_J15001
sequence annotation: BBa_K118012
sequence annotation: BBa_J15001
sequence annotation: BBa_I742151
sequence annotation: BBa_K118003
sequence annotation: BBa_K118002
sequence annotation: BBa_R0011
sequence: https://synbiohub.org/public/igem/BBa_K274110_sequence/1

BBa_I742151
type: Component
persistent identity: component2021368
display id: component2021368
version: 1
top level: BBa_K274110
access: public
definition: https://synbiohub.org/public/igem/BBa_I742151/1

BBa_K118003
type: Component
persistent identity: component2021380
display id: component2021380
version: 1
top level: BBa_K274110
access: public
definition: https://synbiohub.org/public/igem/BBa_K118003/1

BBa_K118012
type: Component
persistent identity: component2021367
display id: component2021367
version: 1
top level: BBa_K274110
access: public
definition: https://synbiohub.org/public/igem/BBa_K118012/1

BBa_R0011
type: Component
persistent identity: component2021362
display id: component2021362
version: 1
top level: BBa_K274110
access: public
definition: https://synbiohub.org/public/igem/BBa_R0011/1

BBa_K118002
type: Component
persistent identity: component2021373
display id: component2021373
version: 1
top level: BBa_K274110
access: public
definition: https://synbiohub.org/public/igem/BBa_K118002/1

BBa_J15001
type: Component
persistent identity: component2021376
display id: component2021376
version: 1
top level: BBa_K274110
access: public
definition: https://synbiohub.org/public/igem/BBa_J15001/1

BBa_J15001
type: Component
persistent identity: component2021371
display id: component2021371
version: 1
top level: BBa_K274110
access: public
definition: https://synbiohub.org/public/igem/BBa_J15001/1

BBa_K118012
type: SequenceAnnotation
persistent identity: annotation2021367
display id: annotation2021367
version: 1
top level: BBa_K274110
location: https://synbiohub.org/public/igem/BBa_K274110/annotation2021367/range2021367/1
component: BBa_K118012

https://synbiohub.org/public/igem/BBa_K274110/annotation2021367/range2021367/1
type: Range
persistent identity: range2021367
display id: range2021367
version: 1
direction: direction/reverse
top level: BBa_K274110
start: 64
end: 67
orientation: inline

BBa_J15001
type: SequenceAnnotation
persistent identity: annotation2021376
display id: annotation2021376
version: 1
top level: BBa_K274110
location: https://synbiohub.org/public/igem/BBa_K274110/annotation2021376/range2021376/1
component: BBa_J15001

https://synbiohub.org/public/igem/BBa_K274110/annotation2021376/range2021376/1
type: Range
persistent identity: range2021376
display id: range2021376
version: 1
direction: direction/reverse
top level: BBa_K274110
start: 1951
end: 1960
orientation: inline

BBa_J15001
type: SequenceAnnotation
persistent identity: annotation2021371
display id: annotation2021371
version: 1
top level: BBa_K274110
location: https://synbiohub.org/public/igem/BBa_K274110/annotation2021371/range2021371/1
component: BBa_J15001

https://synbiohub.org/public/igem/BBa_K274110/annotation2021371/range2021371/1
type: Range
persistent identity: range2021371
display id: range2021371
version: 1
direction: direction/reverse
top level: BBa_K274110
start: 994
end: 1003
orientation: inline

BBa_I742151
type: SequenceAnnotation
persistent identity: annotation2021368
display id: annotation2021368
version: 1
top level: BBa_K274110
location: https://synbiohub.org/public/igem/BBa_K274110/annotation2021368/range2021368/1
component: BBa_I742151

https://synbiohub.org/public/igem/BBa_K274110/annotation2021368/range2021368/1
type: Range
persistent identity: range2021368
display id: range2021368
version: 1
direction: direction/reverse
top level: BBa_K274110
start: 74
end: 985
orientation: inline

BBa_K118003
type: SequenceAnnotation
persistent identity: annotation2021380
display id: annotation2021380
version: 1
top level: BBa_K274110
location: https://synbiohub.org/public/igem/BBa_K274110/annotation2021380/range2021380/1
component: BBa_K118003

https://synbiohub.org/public/igem/BBa_K274110/annotation2021380/range2021380/1
type: Range
persistent identity: range2021380
display id: range2021380
version: 1
direction: direction/reverse
top level: BBa_K274110
start: 1967
end: 3448
orientation: inline

BBa_K118002
type: SequenceAnnotation
persistent identity: annotation2021373
display id: annotation2021373
version: 1
top level: BBa_K274110
location: https://synbiohub.org/public/igem/BBa_K274110/annotation2021373/range2021373/1
component: BBa_K118002

https://synbiohub.org/public/igem/BBa_K274110/annotation2021373/range2021373/1
type: Range
persistent identity: range2021373
display id: range2021373
version: 1
direction: direction/reverse
top level: BBa_K274110
start: 1010
end: 1942
orientation: inline

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation2021362
display id: annotation2021362
version: 1
top level: BBa_K274110
location: https://synbiohub.org/public/igem/BBa_K274110/annotation2021362/range2021362/1
component: BBa_R0011

https://synbiohub.org/public/igem/BBa_K274110/annotation2021362/range2021362/1
type: Range
persistent identity: range2021362
display id: range2021362
version: 1
direction: direction/forward
top level: BBa_K274110
start: 1
end: 54
orientation: inline

BBa_K1943007
type: ComponentDefinition
persistent identity: BBa K1943007
display id: BBa_K1943007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: spisPink, Pink chromoprotein reporter system (medium Promoter , weak RBS)
creator: Shixin Lu, Shiqiang Tang
created: 2016-08-28T11:00:00Z
modified: 2016-10-21T08:40:55Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2410_
m user id: 30006
owner id: 29845
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Team Uppsala 2012 chromoprotein attracts many interests because it's more convenient than fluorescent protein for it can be observed with naked eyes. However, characterized data of these proteins are few. Because a single coding region is on the plasmid, we constructed BBa_K1943007 with chromoprotein spisPink(BBa_K1033932) this year and did characterization. We've constructed a series of plasmids which are all in the same pattern: a strong/weak promoter, a strong/weak RBS and a chromoprotein. We want to monitor the speed of expression of these plasmids in normal incubation conditions (like 37℃ overnight for LB agar plate and 37℃ 180rpm for LB broth). The expression speed and strength of the constructed biobricks will be carefully monitored and gave others a relative scale for using chromoprotein as a reporter gene. For detailed characterization data, see the experience page.
mutable notes: no
mutable provenance: basic parts
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_B0015
component: BBa_K608007
component: BBa_K1033932
sequence annotation: BBa_B0015
sequence annotation: BBa_K1033932
sequence annotation: BBa_K608007
sequence: https://synbiohub.org/public/igem/BBa_K1943007_sequence/1

BBa_B0015
type: Component
persistent identity: component2481942
display id: component2481942
version: 1
top level: BBa_K1943007
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_K608007
type: Component
persistent identity: component2481933
display id: component2481933
version: 1
top level: BBa_K1943007
access: public
definition: https://synbiohub.org/public/igem/BBa_K608007/1

BBa_K1033932
type: Component
persistent identity: component2481935
display id: component2481935
version: 1
top level: BBa_K1943007
access: public
definition: BBa_K1033932

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2481942
display id: annotation2481942
version: 1
top level: BBa_K1943007
location: https://synbiohub.org/public/igem/BBa_K1943007/annotation2481942/range2481942/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K1943007/annotation2481942/range2481942/1
type: Range
persistent identity: range2481942
display id: range2481942
version: 1
direction: direction/reverse
top level: BBa_K1943007
start: 750
end: 878
orientation: inline

BBa_K1033932
type: SequenceAnnotation
persistent identity: annotation2481935
display id: annotation2481935
version: 1
top level: BBa_K1943007
location: https://synbiohub.org/public/igem/BBa_K1943007/annotation2481935/range2481935/1
component: BBa_K1033932

https://synbiohub.org/public/igem/BBa_K1943007/annotation2481935/range2481935/1
type: Range
persistent identity: range2481935
display id: range2481935
version: 1
direction: direction/reverse
top level: BBa_K1943007
start: 64
end: 741
orientation: inline

BBa_K608007
type: SequenceAnnotation
persistent identity: annotation2481933
display id: annotation2481933
version: 1
top level: BBa_K1943007
location: https://synbiohub.org/public/igem/BBa_K1943007/annotation2481933/range2481933/1
component: BBa_K608007

https://synbiohub.org/public/igem/BBa_K1943007/annotation2481933/range2481933/1
type: Range
persistent identity: range2481933
display id: range2481933
version: 1
direction: direction/reverse
top level: BBa_K1943007
start: 1
end: 57
orientation: inline

BBa_K1838007
type: ComponentDefinition
persistent identity: BBa K1838007
display id: BBa_K1838007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: MLC Glucose Sensitive Yellow Chromoprotein Construct
creator: Connor McFadden
created: 2015-09-17T11:00:00Z
modified: 2016-01-21T02:19:56Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2264_
m user id: 4206
owner id: 26050
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This composite part features our MLC2 construct combined via 3A assembly with a yellow chromoprotein+RBS part (BBa_K1033931). During transcription, the MLC2 binding site is bound by the protein MLC, inhibiting production of yellow chromoprotein. As glucose concentration increases in the cell, more MLC is uptaken and bound to the membrane by a mechanism involving the transmembrane glucose permease called IICBGlu. This means that higher glucose concentrations subsequently result in higher expression levels of the yellow chromoprotein and thus makes it a glucose-inducible chromoprotein producing part. Specifically, this part tests the hypothesis for how MLC affects the inducibility of an average strength promoter when placed downstream of the base promoter sequence.
mutable notes: One issue to consider was where to place the MLC binding site and what strength promoter to use as a base. This was solved by creating multiple variants of the promorer (MLC1-4). This part may be improved by considering how the RBS is affected by the position of the MLC binding site.
mutable provenance: 1. Plumbridge, Jacqueline. ???DNA Binding Sites for the MLC and NagC Proteins: Regulation of nagE, Encoding the N-Acetylglucosamine-Specific Transporter inEscherichia Coli.??? Nucleic Acids Research 29.2 (2001): 506???514. Print. 2. http://parts.igem.org/Part:BBa_J23110 3. http://parts.igem.org/Part:BBa_K1033931
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1838003
component: BBa_K1033931
sequence annotation: BBa_K1838003
sequence annotation: BBa_K1033931
sequence: https://synbiohub.org/public/igem/BBa_K1838007_sequence/1

BBa_K1838003
type: Component
persistent identity: component2473533
display id: component2473533
version: 1
top level: BBa_K1838007
access: public
definition: https://synbiohub.org/public/igem/BBa_K1838003/1

BBa_K1033931
type: Component
persistent identity: component2473538
display id: component2473538
version: 1
top level: BBa_K1838007
access: public
definition: https://synbiohub.org/public/igem/BBa_K1033931/1

BBa_K1838003
type: SequenceAnnotation
persistent identity: annotation2473533
display id: annotation2473533
version: 1
top level: BBa_K1838007
location: https://synbiohub.org/public/igem/BBa_K1838007/annotation2473533/range2473533/1
component: BBa_K1838003

https://synbiohub.org/public/igem/BBa_K1838007/annotation2473533/range2473533/1
type: Range
persistent identity: range2473533
display id: range2473533
version: 1
direction: direction/reverse
top level: BBa_K1838007
start: 1
end: 58
orientation: inline

BBa_K1033931
type: SequenceAnnotation
persistent identity: annotation2473538
display id: annotation2473538
version: 1
top level: BBa_K1838007
location: https://synbiohub.org/public/igem/BBa_K1838007/annotation2473538/range2473538/1
component: BBa_K1033931

https://synbiohub.org/public/igem/BBa_K1838007/annotation2473538/range2473538/1
type: Range
persistent identity: range2473538
display id: range2473538
version: 1
direction: direction/reverse
top level: BBa_K1838007
start: 67
end: 783
orientation: inline

amilCP
type: ComponentDefinition
persistent identity: BBa K592009
display id: BBa_K592009
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: amilCP, blue chromoprotein
creator: Lei Sun
created: 2011-09-17T11:00:00Z
modified: 2015-05-08T01:12:48Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _763_
m user id: 0
owner id: 7929
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: This chromoprotein, amilCP, naturally exhibits very strong color when expressed. The color is blue/purple and is visible to naked eye, thereby requiring no instruments to observe. This DNA was provided by Jeffrey Miller at UCLA. It was made BioBrick-compatible after removal of one illegal internal restriction site (EcoRI).
mutable notes: Illegal internal restriction site had to be removed (EcoRI).
mutable provenance: Acropora millepora
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: amilCP
sequence: https://synbiohub.org/public/igem/BBa_K592009_sequence/1

amilCP
type: SequenceAnnotation
persistent identity: annotation2131628
display id: annotation2131628
version: 1
top level: amilCP
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K592009/annotation2131628/range2131628/1

https://synbiohub.org/public/igem/BBa_K592009/annotation2131628/range2131628/1
type: Range
persistent identity: range2131628
display id: range2131628
version: 1
direction: direction/forward
top level: amilCP
start: 1
end: 666
orientation: inline

BBa_K1685008
type: ComponentDefinition
persistent identity: BBa K1685008
display id: BBa_K1685008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: aeBlue with AAV tag
creator: ITB_Indonesia 2015
created: 2015-09-15T11:00:00Z
modified: 2016-01-25T11:15:15Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2103_
m user id: 4206
owner id: 25100
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This part encodes a chromoprotein, aeBlue, with AAV degradation tag
mutable notes: The aeBlue sequence was codon-optimised using http://sg.idtdna.com/CodonOpt. The degradation tag is based on Edinburgh 2014.
mutable provenance: The BBa_K1685005 part is given LAA-degradation tag designed by Edinburgh 2014 team.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1685008_sequence/1

BBa_K1943002
type: ComponentDefinition
persistent identity: BBa K1943002
display id: BBa_K1943002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: gfasPurple, purple chromoprotein reporter system (Strong Promoter, Strong RBS)
creator: Shixin Lu, Shiqiang Tang
created: 2016-08-28T11:00:00Z
modified: 2016-10-21T08:03:17Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2410_
m user id: 30006
owner id: 29845
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Team Uppsala 2012 chromoprotein attracts many interests because it's more convenient than fluorescent protein for it can be observed with naked eyes. However, characterized data of these proteins are few. Because a single coding region is on the plasmid, we constructed BBa_K1943002 with chromoprotein gfasPurple(BBa_K1033919) this year and did characterization. We've constructed a series of plasmids which are all in the same pattern: a strong/weak promoter, a strong/weak RBS and a chromoprotein. We want to monitor the speed of expression of these plasmids in normal incubation conditions (like 37℃ overnight for LB agar plate and 37℃ 180rpm for LB broth). The expression speed and strength of the constructed biobricks will be carefully monitored and gave others a relative scale for using chromoprotein as a reporter gene. For detailed characterization data, see the experience page.
mutable notes: no
mutable provenance: basic parts
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_K1033919
component: BBa_B0015
component: BBa_K880005
sequence annotation: BBa_K1033919
sequence annotation: BBa_K880005
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_K1943002_sequence/1

BBa_K1033919
type: Component
persistent identity: component2512726
display id: component2512726
version: 1
top level: BBa_K1943002
access: public
definition: gfasPurple

BBa_B0015
type: Component
persistent identity: component2512733
display id: component2512733
version: 1
top level: BBa_K1943002
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_K880005
type: Component
persistent identity: component2512724
display id: component2512724
version: 1
top level: BBa_K1943002
access: public
definition: https://synbiohub.org/public/igem/BBa_K880005/1

BBa_K1033919
type: SequenceAnnotation
persistent identity: annotation2512726
display id: annotation2512726
version: 1
top level: BBa_K1943002
location: https://synbiohub.org/public/igem/BBa_K1943002/annotation2512726/range2512726/1
component: BBa_K1033919

https://synbiohub.org/public/igem/BBa_K1943002/annotation2512726/range2512726/1
type: Range
persistent identity: range2512726
display id: range2512726
version: 1
direction: direction/reverse
top level: BBa_K1943002
start: 62
end: 730
orientation: inline

BBa_K880005
type: SequenceAnnotation
persistent identity: annotation2512724
display id: annotation2512724
version: 1
top level: BBa_K1943002
location: https://synbiohub.org/public/igem/BBa_K1943002/annotation2512724/range2512724/1
component: BBa_K880005

https://synbiohub.org/public/igem/BBa_K1943002/annotation2512724/range2512724/1
type: Range
persistent identity: range2512724
display id: range2512724
version: 1
direction: direction/reverse
top level: BBa_K1943002
start: 1
end: 55
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2512733
display id: annotation2512733
version: 1
top level: BBa_K1943002
location: https://synbiohub.org/public/igem/BBa_K1943002/annotation2512733/range2512733/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K1943002/annotation2512733/range2512733/1
type: Range
persistent identity: range2512733
display id: range2512733
version: 1
direction: direction/reverse
top level: BBa_K1943002
start: 739
end: 867
orientation: inline

aeBlue
type: ComponentDefinition
persistent identity: BBa K864403
display id: BBa_K864403
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: J23110-B0034-aeBlue (chromoprotein) (E.coli optimized)
creator: Olle Nordesj??
created: 2012-09-24T11:00:00Z
modified: 2015-05-08T01:13:37Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1124_
m user id: 0
owner id: 14206
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: aeBlue is a blue chromoprotein extracted from the basal disk of a beadlet anemone Actinia equina. The protein has an absorption maximum at 597nm and a deep blue colour. The protein aeBlue is of the GFP family with significant sequence similarities. This part is a functionally active variant of BBa_K864401.
mutable notes: The promoter J23110 is sufficiently strong to yield expression levels making the blue protein clearly visible and eventually opaque.
mutable provenance: The protein was discovered, extracted and analyzed in (Shkrob, 2005). It was synthesized by Bioneer Corporation, codon optimized for E.coli.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_B0034
component: BBa_K864401
component: BBa_J23110
sequence annotation: BBa_J23110
sequence annotation: BBa_K864401
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K864403_sequence/1

BBa_B0034
type: Component
persistent identity: component2198625
display id: component2198625
version: 1
top level: aeBlue
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K864401
type: Component
persistent identity: component2198627
display id: component2198627
version: 1
top level: aeBlue
access: public
definition: aeBlue

BBa_J23110
type: Component
persistent identity: component2198623
display id: component2198623
version: 1
top level: aeBlue
access: public
definition: https://synbiohub.org/public/igem/BBa_J23110/1

BBa_J23110
type: SequenceAnnotation
persistent identity: annotation2198623
display id: annotation2198623
version: 1
top level: aeBlue
location: https://synbiohub.org/public/igem/BBa_K864403/annotation2198623/range2198623/1
component: BBa_J23110

https://synbiohub.org/public/igem/BBa_K864403/annotation2198623/range2198623/1
type: Range
persistent identity: range2198623
display id: range2198623
version: 1
direction: direction/reverse
top level: aeBlue
start: 1
end: 35
orientation: inline

BBa_K864401
type: SequenceAnnotation
persistent identity: annotation2198627
display id: annotation2198627
version: 1
top level: aeBlue
location: https://synbiohub.org/public/igem/BBa_K864403/annotation2198627/range2198627/1
component: BBa_K864401

https://synbiohub.org/public/igem/BBa_K864403/annotation2198627/range2198627/1
type: Range
persistent identity: range2198627
display id: range2198627
version: 1
direction: direction/reverse
top level: aeBlue
start: 62
end: 760
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2198625
display id: annotation2198625
version: 1
top level: aeBlue
location: https://synbiohub.org/public/igem/BBa_K864403/annotation2198625/range2198625/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K864403/annotation2198625/range2198625/1
type: Range
persistent identity: range2198625
display id: range2198625
version: 1
direction: direction/reverse
top level: aeBlue
start: 44
end: 55
orientation: inline

BBa_K1943004
type: ComponentDefinition
persistent identity: BBa K1943004
display id: BBa_K1943004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: eforRed, Red chromoprotein reporter system (Strong Promoter, Strong RBS)
creator: Shixin Lu, Shiqiang Tang
created: 2016-08-28T11:00:00Z
modified: 2016-10-21T08:38:25Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2410_
m user id: 30006
owner id: 29845
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Team Uppsala 2012 chromoprotein attracts many interests because it's more convenient than fluorescent protein for it can be observed with naked eyes. However, characterized data of these proteins are few. Because a single coding region is on the plasmid, we constructed BBa_K1943004 with chromoprotein eforRed(BBa_K592012) this year and did characterization. We've constructed a series of plasmids which are all in the same pattern: a strong/weak promoter, a strong/weak RBS and a chromoprotein. We want to monitor the speed of expression of these plasmids in normal incubation conditions (like 37℃ overnight for LB agar plate and 37℃ 180rpm for LB broth). The expression speed and strength of the constructed biobricks will be carefully monitored and gave others a relative scale for using chromoprotein as a reporter gene. For detailed characterization data, see the experience page.
mutable notes: no
mutable provenance: basic parts
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_B0015
component: BBa_K880005
component: BBa_K592012
sequence annotation: BBa_K592012
sequence annotation: BBa_K880005
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_K1943004_sequence/1

BBa_B0015
type: Component
persistent identity: component2512746
display id: component2512746
version: 1
top level: BBa_K1943004
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_K880005
type: Component
persistent identity: component2512737
display id: component2512737
version: 1
top level: BBa_K1943004
access: public
definition: https://synbiohub.org/public/igem/BBa_K880005/1

BBa_K592012
type: Component
persistent identity: component2512739
display id: component2512739
version: 1
top level: BBa_K1943004
access: public
definition: eforRed

BBa_K592012
type: SequenceAnnotation
persistent identity: annotation2512739
display id: annotation2512739
version: 1
top level: BBa_K1943004
location: https://synbiohub.org/public/igem/BBa_K1943004/annotation2512739/range2512739/1
component: BBa_K592012

https://synbiohub.org/public/igem/BBa_K1943004/annotation2512739/range2512739/1
type: Range
persistent identity: range2512739
display id: range2512739
version: 1
direction: direction/reverse
top level: BBa_K1943004
start: 62
end: 742
orientation: inline

BBa_K880005
type: SequenceAnnotation
persistent identity: annotation2512737
display id: annotation2512737
version: 1
top level: BBa_K1943004
location: https://synbiohub.org/public/igem/BBa_K1943004/annotation2512737/range2512737/1
component: BBa_K880005

https://synbiohub.org/public/igem/BBa_K1943004/annotation2512737/range2512737/1
type: Range
persistent identity: range2512737
display id: range2512737
version: 1
direction: direction/reverse
top level: BBa_K1943004
start: 1
end: 55
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2512746
display id: annotation2512746
version: 1
top level: BBa_K1943004
location: https://synbiohub.org/public/igem/BBa_K1943004/annotation2512746/range2512746/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K1943004/annotation2512746/range2512746/1
type: Range
persistent identity: range2512746
display id: range2512746
version: 1
direction: direction/reverse
top level: BBa_K1943004
start: 751
end: 879
orientation: inline

BBa_K1033916
type: ComponentDefinition
persistent identity: BBa K1033916
display id: BBa_K1033916
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: amajLime, yellow-green chromoprotein
creator: Sabri Jamal
created: 2013-08-24T11:00:00Z
modified: 2015-05-08T01:08:49Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1340_
m user id: 0
owner id: 10137
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This chromoprotein from the coral Anemonia majano, amajLime, naturally exhibits strong color when expressed.
mutable notes: Synthesized and codon optimized for E coli by GenScript.
mutable provenance: The anemone Anemonia majano.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: amajLime
sequence: https://synbiohub.org/public/igem/BBa_K1033916_sequence/1

amajLime
type: SequenceAnnotation
persistent identity: annotation2332103
display id: annotation2332103
version: 1
top level: BBa_K1033916
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1033916/annotation2332103/range2332103/1

https://synbiohub.org/public/igem/BBa_K1033916/annotation2332103/range2332103/1
type: Range
persistent identity: range2332103
display id: range2332103
version: 1
direction: direction/forward
top level: BBa_K1033916
start: 1
end: 690
orientation: inline

yA
type: ComponentDefinition
persistent identity: BBa K678035
display id: BBa_K678035
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: yA (green pigment) from Aspergillus nidulans
creator: DTU-Denmark-2
created: 2011-09-03T11:00:00Z
modified: 2015-05-08T01:13:04Z
discontinued: false
dominant: false
experience: experience/None
group u list: _882_
m user id: 0
owner id: 8341
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: The gene yA from Aspergillus nidulans encodes a p-diphenol oxidase (laccase) that converts a yellow pigment precursor to the mature green form. The green pigment is located in the walls of the conidia and protects the spores from damage by ultraviolet light. A yA mutant produces yellow spores.
mutable notes: Compatible with the Plug'n'Play assembly standard
mutable provenance: Aspergillus nidulans genomic DNA
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Linker 2
sequence annotation: yA
sequence: https://synbiohub.org/public/igem/BBa_K678035_sequence/1

Linker 2
type: SequenceAnnotation
persistent identity: annotation2127401
display id: annotation2127401
version: 1
top level: yA
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K678035/annotation2127401/range2127401/1

https://synbiohub.org/public/igem/BBa_K678035/annotation2127401/range2127401/1
type: Range
persistent identity: range2127401
display id: range2127401
version: 1
direction: direction/forward
top level: yA
start: 1
end: 8
orientation: inline

yA
type: SequenceAnnotation
persistent identity: annotation2127402
display id: annotation2127402
version: 1
top level: yA
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K678035/annotation2127402/range2127402/1

https://synbiohub.org/public/igem/BBa_K678035/annotation2127402/range2127402/1
type: Range
persistent identity: range2127402
display id: range2127402
version: 1
direction: direction/forward
top level: yA
start: 9
end: 2156
orientation: inline