Lead Promoter (Part 4) messed up EcoRI site
Chromium responsive promoter under T7-promoter with UTR-sfGFP
T7 controlled Chromium responsive promoter with 5??untranslated region and sfGFP for detection
Nickel responsive promoter with T7-promoter and UTR-sfGFP
This is just the promoter region of our part pCusC mKate. This promoter is copper sensitive via the CusS/CusR two component system.
Much longer than a version in the registry previously.
Cd sensitive promoter with Phi delta activator, PO promoter, and GFP
The Cadmium sensitive promoter is combined with amplifiers from the 2009 Cambridge iGEM team to increase the sensitivity to cadmium. It uses GFP as a reporter.
This composite part contains only other biobrick parts.
This is the copper sensitive promoter pCopA (controlled by CueR) in front of an RBS and a sfGFP reporter
Promoter from E. coli that controls the AceB gene, involved in iron uptake and iron sensitive. FUR (Ferric Uptake Regulation) is a transcriptional repressor of genes involved in iron homeostasis. In presence of iron, the FUR protein changes its conformation and dimerizes. This leads to the binding to the DNA on a FUR binding site, thus inhibiting the mRNA transcription. Why would you use this part? This promoter allows you to have an iron-sensitive behaviour. In fact, in the presence of iron, the Fes promoter region decreases the expression of the downstream gene.
We blindly extracted the 300 bp sequence upstream of the AceB gene in E. coli. It contains a putative FUR binding site and a putative RBS.
pCopA with plasmid constitutively expressed CueR (no reporter)
Codon optimised. Designed for biobrick assembly with reporter of choice
Rcn is a promoter that is activated by cobalt. A repressor is fixed to the promoter by default, and cobalt binds to this repressor, which activates the gene under control of this promoter. Rcn is also activated by nickel, but we have not characterized this aspect.
An internal iGEM restriction site had to be mutated by QuickChange mutagenesis.
Copper activator under control constitutive promoter and strong RBS and Copper responsive promoter w
This device consists of the Aktivator CueR for the copper responsive promoter CopAP and mRFP for detection. It works as a copper sensor
activator and promoter are part of ????E.coli`` K12 copper efflux system
FP:GTTTCTTCGAATTCGCGGCCGCTTCTAGATTGACTCTGTAGTTGCTACA VP:GTTTCTTCCTGCAGCGGCCGCTACTAGT ATTGCACACC CTGTAGCAAC TACAGA
This part was isolated from Tn21. When regulated by its cognate transcription factor MerR, it will represent a dose-response manner in response to the concentration of mercury.
copper promoter + RBS + ribB + terminator
Nickel repressor under control of constitutive promoter and strong RBS and Nickel responsive promote
pCopA TAT Csp1 sfGFP with divergent CueR
pCopA TAT Csp1 sfGFP with plasmid constitutively expressed CueR
This part is the chromoprotein amilCP from Acropora millepora under control of the mercury sensitive promoter PmerT.
This part is designed for quantification and visual detection of high mercury concentrations in water.
This part is a composite of BBa_K346002 and BBa_K592009 from the 2010 Peking and 2011 Uppsala iGEM teams respectively.
Ferric uptake repressor box (Fur box). When the ferric uptake repressor binds to it, it represses transcription.
Fur boxes differ, we used the consensus sequence which per se is not an actual sequence occuring within B. subtilis but rather the strongest sequence that Fur protein would bind to.
Bacillus subtilis Fur box consensus sequence.
Promoter region from Acidithiobacillus ferrooxidans rus operon
A bi-directional promoter affected by SmtB protein
smt O-P is a promoter which has two inverted repeats, designated S1/S2 and S3/S4, in the overlapping promoter/operator sites between gene smtA and gene smtB. The full promoter/operator DNA binds two SmtB dimmers forming a S1/S2-SmtB:SmtB-S3/S4 bridge complex. After binding with smtB, smt O-P represses the expression of downstream genes.
We got smt O-P and smtA, a fragment coding MT-like to sequester metal ions from smt locus by PCR and overlaped two fragments,smtB-smt O-P and pigment gene.Binding with smtB, smt O-P represses the expression of reporter genes.In the presence of heavy metal,the pigment and SmtA can expression normally.
smt O-P is a part of smt locus from Synechococcus PCC7942.
Mercury responsive promoter with T7-promoter and UTR-sfGFP
Mercury responsive Promoter with 5?? untranslated region and superfolding GFP
mRFP under control of copper responsive promoter
A copper iduceble promoter with RFP for detection
CopAp is a copper induceble promoter out of ``E.coli`` K12
We digested the promoter responsive to Cadmium (BBa_K174015) with EcoRI and SpeI and digested the GFP plasmid (BBa_I13401) with XbaI and PstI. The promoter was Chloramphenicol resistant and the GFP was Ampicillin resistant. These digested fragments were mixed and ligated to the provided, linearized pSB3C1 plasmid. The ligation mix was grown under Chloramphenicol selection. The resulting colonies were tested for responsive GFP production from the addition of Cadmium and Arsenic, respectively.
This part is a combination of 2007 Brown's iGEM part BBa_K174015 and MIT's part (from 2005) I13401.
Nickel responsive Promoter with mRFP for detection
Promoter from E. coli that controls the Fes gene, involved in iron uptake and iron sensitive. FUR (Ferric Uptake Regulation) is a transcriptional repressor of genes involved in iron homeostasis. In presence of iron, the FUR protein changes its conformation and dimerizes. This leads to the binding to the DNA on a FUR binding site, thus inhibiting the mRNA transcription. Why would you use this part? This promoter allows you to have an iron-sensitive behaviour. In fact, in the presence of iron, the Fes promoter region decreases the expression of the downstream gene.
We blindly extracted the 298 bp sequence upstream of the Fes gene in E. coli. It contains a putative FUR binding site and a putative RBS.
This part is the chromoprotein amilCP from Acropora millepora under control of the cobalt and nickel sensitive promoter Prcn.
This part is designed to quantify and detect high concentrations of cobalt or nickel in water.
This is a composite part of the biobricks BBa_K540001 and BBa_K592009 from the 2011 Lyon Biosciences and 2011 Uppsala iGEM teams respectfully.
UTR-sfGFP controlled by T7-promoter and lead responsive promoter
lead induceble promoter under the control of T7 Promoter with mRFP for detection
This part consists of T7 promoter lead induceble Promoter PbrA from ''Cupriavidus metallidurans CH34'' and mRFP for detection
Lead responsive promoter with T7-promoter and UTR-sfGFP
Promoter from E. coli that controls the YncE gene, involved in iron uptake and iron sensitive. FUR (Ferric Uptake Regulation) is a transcriptional repressor of genes involved in iron homeostasis. In presence of iron, the FUR protein changes its conformation and dimerizes. This leads to the binding to the DNA on a FUR binding site, thus inhibiting the mRNA transcription. Why would you use this part? This promoter allows you to have an iron-sensitive behaviour. In fact, in the presence of iron, the YncE promoter region decreases the expression of the downstream gene.
We blindly extracted the 300 bp sequence upstream of the YncE gene in E. coli. It contains a putative FUR binding site and a putative RBS.
pCopA MymT sfGFP with plasmid constitutively expressed CueR
Promoter from the copper-sensetive CusR/CusS two component signal system. (E.Coli)
Sequence from the NCBI database, with added BioBricks prefix and suffix.
This part purely holds the binding site for ArsR protein which can be released when bound to different metals. Hence this part can be combined with any promoter to sense metals that can bind to ArsR protein. When added in front of a promoter with another metal sensor's protein's binding site, it can form an AND gate to sensitively detect specific metals.
It was developed mainly to use combinatorial approaches for metal sensing.
Nickel responsive promoter with UTR-sfGFP
Nickel respnsive promoter with 5?? untranslated region and sfGFP for detection
Lead repressor under control of constitutive promoter and strong RBS and lead responsive promoter wi
Repressor for lead responsive promoter under the control of konstitutive promoter and lead responsive promoter with 5??untranslated region and sfGFP for detection. As a lead biosensor
genomic sequence out of ????Cupriavidus metallidurans???? CH34
zinTp (Cd2+ sensing promoter) (Fw:Lac promoter(-) ver.)
Daiki Haraguchi,Naoki Saito,Yoshiharu Otaki,Ryuhei Minei
Copper responsive promoter with T7-promoter and UTR-sfGFP
pCopA MymT with divergent expressed CueR
pCopA MymT with plasmid constitutively expressed CueR
UTR-sfGFP under control of Copper responsive promoter
copper responsive promoter with 5?? untranslated region and sfGFP for detection
copper responsive promoter of ????E.coli`` with sfGFP for detection
Lead Promoter with GFP Reporter (Lead Detector)
We digested the promoter responsive to Lead (BBa_I721001) with EcoRI and SpeI and digested the GFP plasmid (BBa_I13401) with XbaI and PstI. The promoter and reporter were Ampicillin resistant. These digested fragments were mixed and ligated to the provided, linearized pSB1C3 plasmid. The ligation mix was grown under Chloramphenicol selection. The resulting colonies were tested for responsive GFP production following the addition of Lead Nitrate.
The composite part was formed from BBa_I721001 and BBa_I13401.
MerR activator under constitutive promoter and induceble merT promoter with 5 UTR -sfGFP
Chromium repressor under control of constitutive promoter and strong RBS,chromium responsive promote
a divice consisting of chromium repressor under the control of konstitutive promoter, the chromium responsive promoter a 5??untranslated region and sfGFP for detectin
this device is inspired by K10580008 a chromium responsive system out of ????Ochrobactrum tritici 5bvl1????. We cominedd the chromium VI responsive promoter with a ????E.coli???? codon optimised repressor sequence
lead responsive promoter with 5??untranslated region and sfGFP for detection
genomic sequence of ``cupriavidus metallidurans CH34``
In ''B. subtilis'' CadA system works as an efflux system to take the metals out in the cell. It is normally repressed by CzrA repressor protein. When certain metals get inside the cell, they can bind to CzrA and derepress the promoter expressing CadA. Hence metals are taken out by the efflux system. By taking the promoter of CadA system, we created a generic metal sensor. Hence when it is combined with coding sequences of other genes, it will regulate the expression of these genes upon sensing metals.
It was developed mainly to use combinatorial approaches for metal sensing
It is taken from ''B. subtilis'' ''cadA'' promoter.
lead responsive promoter with mRFP for detection
lead responsive promoter of ????Cupriavidus metallidurans???? CH34
Arsenic Promoter with GFP Reporter (Arsenic Detector)
We digested the promoter responsive to Arsenic(BBa_K174015) with EcoRI and SpeI and digested the GFP plasmid (BBa_I13401) with XbaI and PstI. The promoter was Chloramphenicol resistant and the GFP was Ampicillin resistant. These digested fragments were mixed and ligated to the provided, linearized pSB3C1 plasmid. The ligation mix was grown under Chloramphenicol selection. The resulting colonies were tested for responsive GFP production after the addition of Cadmium Chloride since the promoter is responsive to multiple heavy metals.
This is a composite part formed from parts BBA_K174015 and BBa_I13401.
Mercury responsive promoter with UTR-sfGFP
mercury responsive promoter with 5??untranslated region and sfGFP for detection
Chromium responsive promoter with UTR+RBS and sfGFP
Chromium responsive promoter with 5?? untremslated region and sfGFP for detection
pCusC mKate2. copper sensitive promoter with RFP reporter gene