//function/structures
type: Collection
persistent identity: function structures
display id: function_structures
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
owned by: james
owned by: myers
member: LipA-HE
member: LipA-EAK16
member: TAL Unit
member: BBa_K1333312
member: SacB-HE
member: SacB-EAK16
member: BBa_K1666000
member: BBa_K1404007
member: BBa_K1404006
member: BBa_K1404008
member: BBa_K897318

TAL Unit
type: ComponentDefinition
persistent identity: BBa K1453201
display id: BBa_K1453201
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: [ssDsbA]->[RFP]->[Lgt]->[TAL-adapter]
creator: Binhan Hao
created: 2014-10-02T11:00:00Z
modified: 2015-05-08T01:10:30Z
discontinued: true
dominant: false
experience: experience/None
group u list: _1831_
m user id: 0
owner id: 21355
owning group id: 9
sample status: Discontinued
status: status/Deleted
bookmark: true
mutable description: [ssDsbA]->[RFP]->[Lgt]->[TAL-adapter]
mutable notes: TALEs
mutable provenance: BBa_K771101;BBa_K771102; BBa_E1010
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Helix Linker
sequence annotation: TAL-T1
sequence annotation: ssDsbA
sequence annotation: TAL-adapter
sequence annotation: Flexible Linker 3
sequence annotation: TAL-T14
sequence annotation: RFP
sequence annotation: Lgt
sequence annotation: His-tag
sequence: https://synbiohub.org/public/igem/BBa_K1453201_sequence/1

Helix Linker
type: SequenceAnnotation
persistent identity: annotation2392479
display id: annotation2392479
version: 1
top level: TAL Unit
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1453201/annotation2392479/range2392479/1

https://synbiohub.org/public/igem/BBa_K1453201/annotation2392479/range2392479/1
type: Range
persistent identity: range2392479
display id: range2392479
version: 1
direction: direction/forward
top level: TAL Unit
start: 733
end: 780
orientation: inline

TAL-T1
type: SequenceAnnotation
persistent identity: annotation2392482
display id: annotation2392482
version: 1
top level: TAL Unit
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1453201/annotation2392482/range2392482/1

https://synbiohub.org/public/igem/BBa_K1453201/annotation2392482/range2392482/1
type: Range
persistent identity: range2392482
display id: range2392482
version: 1
direction: direction/forward
top level: TAL Unit
start: 1711
end: 1813
orientation: inline

ssDsbA
type: SequenceAnnotation
persistent identity: annotation2392477
display id: annotation2392477
version: 1
top level: TAL Unit
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1453201/annotation2392477/range2392477/1

https://synbiohub.org/public/igem/BBa_K1453201/annotation2392477/range2392477/1
type: Range
persistent identity: range2392477
display id: range2392477
version: 1
direction: direction/forward
top level: TAL Unit
start: 1
end: 57
orientation: inline

TAL-adapter
type: SequenceAnnotation
persistent identity: annotation2392483
display id: annotation2392483
version: 1
top level: TAL Unit
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1453201/annotation2392483/range2392483/1

https://synbiohub.org/public/igem/BBa_K1453201/annotation2392483/range2392483/1
type: Range
persistent identity: range2392483
display id: range2392483
version: 1
direction: direction/forward
top level: TAL Unit
start: 1814
end: 2043
orientation: inline

Flexible Linker 3
type: SequenceAnnotation
persistent identity: annotation2392481
display id: annotation2392481
version: 1
top level: TAL Unit
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1453201/annotation2392481/range2392481/1

https://synbiohub.org/public/igem/BBa_K1453201/annotation2392481/range2392481/1
type: Range
persistent identity: range2392481
display id: range2392481
version: 1
direction: direction/forward
top level: TAL Unit
start: 1654
end: 1710
orientation: inline

TAL-T14
type: SequenceAnnotation
persistent identity: annotation2392484
display id: annotation2392484
version: 1
top level: TAL Unit
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1453201/annotation2392484/range2392484/1

https://synbiohub.org/public/igem/BBa_K1453201/annotation2392484/range2392484/1
type: Range
persistent identity: range2392484
display id: range2392484
version: 1
direction: direction/forward
top level: TAL Unit
start: 2044
end: 2142
orientation: inline

RFP
type: SequenceAnnotation
persistent identity: annotation2392478
display id: annotation2392478
version: 1
top level: TAL Unit
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1453201/annotation2392478/range2392478/1

https://synbiohub.org/public/igem/BBa_K1453201/annotation2392478/range2392478/1
type: Range
persistent identity: range2392478
display id: range2392478
version: 1
direction: direction/forward
top level: TAL Unit
start: 58
end: 732
orientation: inline

Lgt
type: SequenceAnnotation
persistent identity: annotation2392480
display id: annotation2392480
version: 1
top level: TAL Unit
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1453201/annotation2392480/range2392480/1

https://synbiohub.org/public/igem/BBa_K1453201/annotation2392480/range2392480/1
type: Range
persistent identity: range2392480
display id: range2392480
version: 1
direction: direction/forward
top level: TAL Unit
start: 781
end: 1653
orientation: inline

His-tag
type: SequenceAnnotation
persistent identity: annotation2392485
display id: annotation2392485
version: 1
top level: TAL Unit
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1453201/annotation2392485/range2392485/1

https://synbiohub.org/public/igem/BBa_K1453201/annotation2392485/range2392485/1
type: Range
persistent identity: range2392485
display id: range2392485
version: 1
direction: direction/forward
top level: TAL Unit
start: 2143
end: 2160
orientation: inline

SacB-HE
type: ComponentDefinition
persistent identity: BBa K143039
display id: BBa_K143039
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: SacB-Human Elastin (EP20-24-24) Fusion Protein
creator: Qin Qi
created: 2008-09-16T11:00:00Z
modified: 2015-05-08T01:10:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _199_
m user id: 0
owner id: 3429
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Elastin is a polymeric extracellular matrix protein found in tissues that require the ability to extend and recoil. Examples of elastin containing tissues include arteries, lungs, ligaments and skin. Construct EP20-24-24 for human elastin polypeptide consists of distinct exons which code for alternating hydrophobic regions and crosslinking domains from the human elastin polypeptide gene 1. Under appropriate conditions of temperature and ionic strength, elastin polypeptide undergoes a self-aggregation process known as coacervation. Coacervation is usually induced by an increase in temperature and causes the protein to separate from the solution as a second phase. Unlike most proteins which undergo denaturation when the temperature of the solution increases, elastin polypeptides become more ordered through coacervation 2. SacB is a signal peptide used in the Sec-SRP (secretory signal recognition particle) pathway by ''B. subtilis''. Signal peptides are responsible for directing preproteins (secretory proteins with a signal peptide region attached) through an appropriate secretory pathway. In the case of the Sec-SRP signal peptide, they direct preproteins from the cytoplasm into the growth medium. SacB has been successfully used in the secretion of heterologous proteins such as acid-stable α-amylase, cystatin and interleukin-3 by B.subtilis 3.
mutable notes: BioBrick standard was applied to the SacB-Human Elastin(EP20-24-24) Fusion Protein.
mutable provenance: All exons in EP20-24-24 are derived from the human elastin polypeptide gene. EP20-24-24 was used to study the effect of various combinations of exons on coacervation of elastin polypeptide. SacB was identified from the initial part of certain preprotein genes that utilises the the Sec-SRP secretory pathway 4. Both components were synthesised as a fusin protein by GeneArt.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: start
sequence annotation: SacB
sequence annotation: stop
sequence annotation: Human Elastin (EP20-24-24)
sequence: https://synbiohub.org/public/igem/BBa_K143039_sequence/1

start
type: SequenceAnnotation
persistent identity: annotation1992729
display id: annotation1992729
version: 1
top level: SacB-HE
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K143039/annotation1992729/range1992729/1

https://synbiohub.org/public/igem/BBa_K143039/annotation1992729/range1992729/1
type: Range
persistent identity: range1992729
display id: range1992729
version: 1
direction: direction/forward
top level: SacB-HE
start: 1
end: 3
orientation: inline

SacB
type: SequenceAnnotation
persistent identity: annotation1976019
display id: annotation1976019
version: 1
top level: SacB-HE
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K143039/annotation1976019/range1976019/1

https://synbiohub.org/public/igem/BBa_K143039/annotation1976019/range1976019/1
type: Range
persistent identity: range1976019
display id: range1976019
version: 1
direction: direction/forward
top level: SacB-HE
start: 1
end: 96
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1992731
display id: annotation1992731
version: 1
top level: SacB-HE
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K143039/annotation1992731/range1992731/1

https://synbiohub.org/public/igem/BBa_K143039/annotation1992731/range1992731/1
type: Range
persistent identity: range1992731
display id: range1992731
version: 1
direction: direction/forward
top level: SacB-HE
start: 694
end: 696
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1992730
display id: annotation1992730
version: 1
top level: SacB-HE
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K143039/annotation1992730/range1992730/1

https://synbiohub.org/public/igem/BBa_K143039/annotation1992730/range1992730/1
type: Range
persistent identity: range1992730
display id: range1992730
version: 1
direction: direction/forward
top level: SacB-HE
start: 697
end: 699
orientation: inline

Human Elastin (EP20-24-24)
type: SequenceAnnotation
persistent identity: annotation1992732
display id: annotation1992732
version: 1
top level: SacB-HE
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K143039/annotation1992732/range1992732/1

https://synbiohub.org/public/igem/BBa_K143039/annotation1992732/range1992732/1
type: Range
persistent identity: range1992732
display id: range1992732
version: 1
direction: direction/forward
top level: SacB-HE
start: 97
end: 693
orientation: inline

BBa_K897318
type: ComponentDefinition
persistent identity: BBa K897318
display id: BBa_K897318
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Paired termini structure
creator: Muyuan Chen
created: 2012-09-17T11:00:00Z
modified: 2015-05-08T01:13:42Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1162_
m user id: 0
owner id: 12559
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Hair-pin structure can protect antisense FtsZ.
mutable notes: With T7
mutable provenance: no
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Other
role: SO:0000110
sequence annotation: PT2
sequence annotation: PT1
sequence: https://synbiohub.org/public/igem/BBa_K897318_sequence/1

PT2
type: SequenceAnnotation
persistent identity: annotation2194708
display id: annotation2194708
version: 1
top level: BBa_K897318
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K897318/annotation2194708/range2194708/1

https://synbiohub.org/public/igem/BBa_K897318/annotation2194708/range2194708/1
type: Range
persistent identity: range2194708
display id: range2194708
version: 1
direction: direction/forward
top level: BBa_K897318
start: 94
end: 138
orientation: inline

PT1
type: SequenceAnnotation
persistent identity: annotation2194706
display id: annotation2194706
version: 1
top level: BBa_K897318
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K897318/annotation2194706/range2194706/1

https://synbiohub.org/public/igem/BBa_K897318/annotation2194706/range2194706/1
type: Range
persistent identity: range2194706
display id: range2194706
version: 1
direction: direction/forward
top level: BBa_K897318
start: 1
end: 39
orientation: inline

LipA-HE
type: ComponentDefinition
persistent identity: BBa K143035
display id: BBa_K143035
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LipA-Human Elastin (EP20-24-24) Fusion Protein
creator: Qin Qi
created: 2008-09-17T11:00:00Z
modified: 2015-05-08T01:10:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _199_
m user id: 0
owner id: 3429
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Elastin is a polymeric extracellular matrix protein found in tissues that require the ability to extend and recoil. Examples of elastin containing tissues include arteries, lungs, ligaments and skin. Construct EP20-24-24 for human elastin polypeptide consists of distinct exons which code for alternating hydrophobic regions and crosslinking domains from the human elastin polypeptide gene 1. Under appropriate conditions of temperature and ionic strength, elastin polypeptide undergoes a self-aggregation process known as coacervation. Coacervation is usually induced by an increase in temperature and causes the protein to separate from the solution as a second phase. Unlike most proteins which undergo denaturation when the temperature of the solution increases, elastin polypeptides become more ordered through coacervation 2. LipA is a signal peptide from the B.subtilis genome. In general, signal peptides are responsible for directing preproteins (secretory proteins with a signal peptide region attached)through an appropriate secretory pathway3. LipA has been successfully used in the secretion of heterologous proteins such as cutinase by ''B. subtilis''.
mutable notes: BioBrick standard was applied to the LipA-Human Elastin(EP20-24-24) Fusion Protein.
mutable provenance: All exons in EP20-24-24 are derived from the human elastin polypeptide gene. EP20-24-24 was used to study the effect of various combinations of exons on coacervation of elastin polypeptide. LipA originated from the B. subtilis genome4. Both components were produced as a fusion protein by GeneArt.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: start
sequence annotation: LipA
sequence annotation: stop
sequence annotation: Human Elastin (EP20-24-24)
sequence: https://synbiohub.org/public/igem/BBa_K143035_sequence/1

start
type: SequenceAnnotation
persistent identity: annotation1992707
display id: annotation1992707
version: 1
top level: LipA-HE
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K143035/annotation1992707/range1992707/1

https://synbiohub.org/public/igem/BBa_K143035/annotation1992707/range1992707/1
type: Range
persistent identity: range1992707
display id: range1992707
version: 1
direction: direction/forward
top level: LipA-HE
start: 1
end: 3
orientation: inline

LipA
type: SequenceAnnotation
persistent identity: annotation1976410
display id: annotation1976410
version: 1
top level: LipA-HE
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K143035/annotation1976410/range1976410/1

https://synbiohub.org/public/igem/BBa_K143035/annotation1976410/range1976410/1
type: Range
persistent identity: range1976410
display id: range1976410
version: 1
direction: direction/forward
top level: LipA-HE
start: 1
end: 102
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1992710
display id: annotation1992710
version: 1
top level: LipA-HE
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K143035/annotation1992710/range1992710/1

https://synbiohub.org/public/igem/BBa_K143035/annotation1992710/range1992710/1
type: Range
persistent identity: range1992710
display id: range1992710
version: 1
direction: direction/forward
top level: LipA-HE
start: 703
end: 705
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1992709
display id: annotation1992709
version: 1
top level: LipA-HE
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K143035/annotation1992709/range1992709/1

https://synbiohub.org/public/igem/BBa_K143035/annotation1992709/range1992709/1
type: Range
persistent identity: range1992709
display id: range1992709
version: 1
direction: direction/forward
top level: LipA-HE
start: 700
end: 702
orientation: inline

Human Elastin (EP20-24-24)
type: SequenceAnnotation
persistent identity: annotation1992708
display id: annotation1992708
version: 1
top level: LipA-HE
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K143035/annotation1992708/range1992708/1

https://synbiohub.org/public/igem/BBa_K143035/annotation1992708/range1992708/1
type: Range
persistent identity: range1992708
display id: range1992708
version: 1
direction: direction/forward
top level: LipA-HE
start: 103
end: 699
orientation: inline

BBa_K1404008
type: ComponentDefinition
persistent identity: BBa K1404008
display id: BBa_K1404008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: p70-CsgA-His*2, double His-tagged curli generator
creator: Juliette Paillet, Alexandre Duprey, Emiel van der Kouwe
created: 2014-09-23T11:00:00Z
modified: 2015-05-08T01:10:17Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1782_
m user id: 0
owner id: 21195
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: temp
mutable notes: temp
mutable provenance: temp
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: CsgA-His2
sequence annotation: GCT into GCC
sequence annotation: linker
sequence annotation: Beta sheet4
sequence annotation: BBa_K342000
sequence annotation: RBS Paris 2009
sequence annotation: Linker
sequence annotation: Beta sheet5
sequence annotation: Beta sheet2
sequence annotation: CsgD signal
sequence annotation: Beta sheet
sequence annotation: His tag
sequence annotation: SEC signal
sequence: https://synbiohub.org/public/igem/BBa_K1404008_sequence/1

CsgA-His2
type: SequenceAnnotation
persistent identity: annotation2426510
display id: annotation2426510
version: 1
top level: BBa_K1404008
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1404008/annotation2426510/range2426510/1

https://synbiohub.org/public/igem/BBa_K1404008/annotation2426510/range2426510/1
type: Range
persistent identity: range2426510
display id: range2426510
version: 1
direction: direction/forward
top level: BBa_K1404008
start: 92
end: 607
orientation: inline

GCT into GCC
type: SequenceAnnotation
persistent identity: annotation2426516
display id: annotation2426516
version: 1
top level: BBa_K1404008
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K1404008/annotation2426516/range2426516/1

https://synbiohub.org/public/igem/BBa_K1404008/annotation2426516/range2426516/1
type: Range
persistent identity: range2426516
display id: range2426516
version: 1
direction: direction/forward
top level: BBa_K1404008
start: 461
end: 463
orientation: inline

linker
type: SequenceAnnotation
persistent identity: annotation2426520
display id: annotation2426520
version: 1
top level: BBa_K1404008
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1404008/annotation2426520/range2426520/1

https://synbiohub.org/public/igem/BBa_K1404008/annotation2426520/range2426520/1
type: Range
persistent identity: range2426520
display id: range2426520
version: 1
direction: direction/forward
top level: BBa_K1404008
start: 575
end: 586
orientation: inline

Beta sheet4
type: SequenceAnnotation
persistent identity: annotation2426515
display id: annotation2426515
version: 1
top level: BBa_K1404008
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1404008/annotation2426515/range2426515/1

https://synbiohub.org/public/igem/BBa_K1404008/annotation2426515/range2426515/1
type: Range
persistent identity: range2426515
display id: range2426515
version: 1
direction: direction/forward
top level: BBa_K1404008
start: 422
end: 487
orientation: inline

BBa_K342000
type: SequenceAnnotation
persistent identity: annotation2426507
display id: annotation2426507
version: 1
top level: BBa_K1404008
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1404008/annotation2426507/range2426507/1

https://synbiohub.org/public/igem/BBa_K1404008/annotation2426507/range2426507/1
type: Range
persistent identity: range2426507
display id: range2426507
version: 1
direction: direction/forward
top level: BBa_K1404008
start: 1
end: 70
orientation: inline

RBS Paris 2009
type: SequenceAnnotation
persistent identity: annotation2426508
display id: annotation2426508
version: 1
top level: BBa_K1404008
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1404008/annotation2426508/range2426508/1

https://synbiohub.org/public/igem/BBa_K1404008/annotation2426508/range2426508/1
type: Range
persistent identity: range2426508
display id: range2426508
version: 1
direction: direction/forward
top level: BBa_K1404008
start: 79
end: 91
orientation: inline

Linker
type: SequenceAnnotation
persistent identity: annotation2426518
display id: annotation2426518
version: 1
top level: BBa_K1404008
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1404008/annotation2426518/range2426518/1

https://synbiohub.org/public/igem/BBa_K1404008/annotation2426518/range2426518/1
type: Range
persistent identity: range2426518
display id: range2426518
version: 1
direction: direction/forward
top level: BBa_K1404008
start: 545
end: 556
orientation: inline

Beta sheet5
type: SequenceAnnotation
persistent identity: annotation2426517
display id: annotation2426517
version: 1
top level: BBa_K1404008
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1404008/annotation2426517/range2426517/1

https://synbiohub.org/public/igem/BBa_K1404008/annotation2426517/range2426517/1
type: Range
persistent identity: range2426517
display id: range2426517
version: 1
direction: direction/forward
top level: BBa_K1404008
start: 488
end: 544
orientation: inline

Beta sheet2
type: SequenceAnnotation
persistent identity: annotation2426513
display id: annotation2426513
version: 1
top level: BBa_K1404008
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1404008/annotation2426513/range2426513/1

https://synbiohub.org/public/igem/BBa_K1404008/annotation2426513/range2426513/1
type: Range
persistent identity: range2426513
display id: range2426513
version: 1
direction: direction/forward
top level: BBa_K1404008
start: 287
end: 352
orientation: inline

CsgD signal
type: SequenceAnnotation
persistent identity: annotation2426511
display id: annotation2426511
version: 1
top level: BBa_K1404008
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1404008/annotation2426511/range2426511/1

https://synbiohub.org/public/igem/BBa_K1404008/annotation2426511/range2426511/1
type: Range
persistent identity: range2426511
display id: range2426511
version: 1
direction: direction/forward
top level: BBa_K1404008
start: 152
end: 217
orientation: inline

Beta sheet
type: SequenceAnnotation
persistent identity: annotation2426514
display id: annotation2426514
version: 1
top level: BBa_K1404008
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1404008/annotation2426514/range2426514/1

https://synbiohub.org/public/igem/BBa_K1404008/annotation2426514/range2426514/1
type: Range
persistent identity: range2426514
display id: range2426514
version: 1
direction: direction/forward
top level: BBa_K1404008
start: 353
end: 421
orientation: inline

Beta sheet
type: SequenceAnnotation
persistent identity: annotation2426512
display id: annotation2426512
version: 1
top level: BBa_K1404008
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1404008/annotation2426512/range2426512/1

https://synbiohub.org/public/igem/BBa_K1404008/annotation2426512/range2426512/1
type: Range
persistent identity: range2426512
display id: range2426512
version: 1
direction: direction/forward
top level: BBa_K1404008
start: 218
end: 286
orientation: inline

His tag
type: SequenceAnnotation
persistent identity: annotation2426519
display id: annotation2426519
version: 1
top level: BBa_K1404008
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1404008/annotation2426519/range2426519/1

https://synbiohub.org/public/igem/BBa_K1404008/annotation2426519/range2426519/1
type: Range
persistent identity: range2426519
display id: range2426519
version: 1
direction: direction/forward
top level: BBa_K1404008
start: 557
end: 574
orientation: inline

His tag
type: SequenceAnnotation
persistent identity: annotation2426521
display id: annotation2426521
version: 1
top level: BBa_K1404008
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1404008/annotation2426521/range2426521/1

https://synbiohub.org/public/igem/BBa_K1404008/annotation2426521/range2426521/1
type: Range
persistent identity: range2426521
display id: range2426521
version: 1
direction: direction/forward
top level: BBa_K1404008
start: 587
end: 604
orientation: inline

SEC signal
type: SequenceAnnotation
persistent identity: annotation2426509
display id: annotation2426509
version: 1
top level: BBa_K1404008
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1404008/annotation2426509/range2426509/1

https://synbiohub.org/public/igem/BBa_K1404008/annotation2426509/range2426509/1
type: Range
persistent identity: range2426509
display id: range2426509
version: 1
direction: direction/forward
top level: BBa_K1404008
start: 92
end: 151
orientation: inline

SacB-EAK16
type: ComponentDefinition
persistent identity: BBa K143038
display id: BBa_K143038
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: SacB-EAK16-II Fusion Protein
creator: Qin Qi
created: 2008-09-17T11:00:00Z
modified: 2015-05-08T01:10:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _199_
m user id: 0
owner id: 3429
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: EAK16-II is a sixteen amino acid peptide that self-assembles to form β-sheet structures in an aqueous medium. The alternating positive and negative charges (--++--++) are responsible for creating an electrostatic attraction between adjacent peptides 1, triggering self-assembly when the EAK16-II peptides are exposed to physiological media or salt solution. When examined under SEM, a well-ordered nanofibre structure is formed by the association of the EAK16-II peptides and these nanofibres can futher aggregate to form a membranous 3D scaffold. SacB is a signal peptide used in the Sec-SRP (secretory signal recognition particle) pathway by ''B. subtilis''. Signal peptides are responsible for directing preproteins (secretory proteins with a signal peptide region attached) through an appropriate secretory pathway. In the case of the Sec-SRP signal peptide, they direct preproteins from the cytoplasm into the growth medium. SacB has been successfully used in the secretion of heterologous proteins such as acid-stable α-amylase, cystatin and interleukin-3 by B.subtilis 2.
mutable notes: BioBrick standard was applied to the SacB-EAK16-II Fusion Protein.
mutable provenance: EAK16-II is a segment from zuotin, a yeast protein. SacB was identified from the initial part of certain preprotein genes that utilises the the Sec-SRP secretory pathway 3. Both components were synthesised as a fusin protein by GeneArt.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: EAK16-II
sequence annotation: SacB
sequence annotation: start
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_K143038_sequence/1

EAK16-II
type: SequenceAnnotation
persistent identity: annotation1992725
display id: annotation1992725
version: 1
top level: SacB-EAK16
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K143038/annotation1992725/range1992725/1

https://synbiohub.org/public/igem/BBa_K143038/annotation1992725/range1992725/1
type: Range
persistent identity: range1992725
display id: range1992725
version: 1
direction: direction/forward
top level: SacB-EAK16
start: 97
end: 144
orientation: inline

SacB
type: SequenceAnnotation
persistent identity: annotation1976411
display id: annotation1976411
version: 1
top level: SacB-EAK16
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K143038/annotation1976411/range1976411/1

https://synbiohub.org/public/igem/BBa_K143038/annotation1976411/range1976411/1
type: Range
persistent identity: range1976411
display id: range1976411
version: 1
direction: direction/forward
top level: SacB-EAK16
start: 1
end: 96
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation1992726
display id: annotation1992726
version: 1
top level: SacB-EAK16
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K143038/annotation1992726/range1992726/1

https://synbiohub.org/public/igem/BBa_K143038/annotation1992726/range1992726/1
type: Range
persistent identity: range1992726
display id: range1992726
version: 1
direction: direction/forward
top level: SacB-EAK16
start: 1
end: 3
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1992728
display id: annotation1992728
version: 1
top level: SacB-EAK16
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K143038/annotation1992728/range1992728/1

https://synbiohub.org/public/igem/BBa_K143038/annotation1992728/range1992728/1
type: Range
persistent identity: range1992728
display id: range1992728
version: 1
direction: direction/forward
top level: SacB-EAK16
start: 148
end: 150
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1992727
display id: annotation1992727
version: 1
top level: SacB-EAK16
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K143038/annotation1992727/range1992727/1

https://synbiohub.org/public/igem/BBa_K143038/annotation1992727/range1992727/1
type: Range
persistent identity: range1992727
display id: range1992727
version: 1
direction: direction/forward
top level: SacB-EAK16
start: 145
end: 147
orientation: inline

BBa_K1333312
type: ComponentDefinition
persistent identity: BBa K1333312
display id: BBa_K1333312
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: I13458-I13453-RBS-G3P(M13)
creator: Wei Tang
created: 2014-09-23T11:00:00Z
modified: 2015-05-08T01:09:58Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1708_
m user id: 0
owner id: 15896
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: expression PⅢ, which is from M13 phage genome, with the regulation of araBAD promoter.
mutable notes: For the rescure experiment.
mutable provenance: PⅢ is from M13 phage genome.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_I13453
component: BBa_I13458
component: BBa_B0034
component: BBa_K1333002
sequence annotation: BBa_K1333002
sequence annotation: BBa_I13453
sequence annotation: BBa_I13458
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K1333312_sequence/1

BBa_I13453
type: Component
persistent identity: component2386081
display id: component2386081
version: 1
top level: BBa_K1333312
access: public
definition: https://synbiohub.org/public/igem/BBa_I13453/1

BBa_I13458
type: Component
persistent identity: component2386080
display id: component2386080
version: 1
top level: BBa_K1333312
access: public
definition: https://synbiohub.org/public/igem/BBa_I13458/1

BBa_B0034
type: Component
persistent identity: component2386083
display id: component2386083
version: 1
top level: BBa_K1333312
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K1333002
type: Component
persistent identity: component2386087
display id: component2386087
version: 1
top level: BBa_K1333312
access: public
definition: https://synbiohub.org/public/igem/BBa_K1333002/1

BBa_K1333002
type: SequenceAnnotation
persistent identity: annotation2386087
display id: annotation2386087
version: 1
top level: BBa_K1333312
location: https://synbiohub.org/public/igem/BBa_K1333312/annotation2386087/range2386087/1
component: BBa_K1333002

https://synbiohub.org/public/igem/BBa_K1333312/annotation2386087/range2386087/1
type: Range
persistent identity: range2386087
display id: range2386087
version: 1
direction: direction/reverse
top level: BBa_K1333312
start: 1279
end: 2619
orientation: inline

BBa_I13453
type: SequenceAnnotation
persistent identity: annotation2386081
display id: annotation2386081
version: 1
top level: BBa_K1333312
location: https://synbiohub.org/public/igem/BBa_K1333312/annotation2386081/range2386081/1
component: BBa_I13453

https://synbiohub.org/public/igem/BBa_K1333312/annotation2386081/range2386081/1
type: Range
persistent identity: range2386081
display id: range2386081
version: 1
direction: direction/reverse
top level: BBa_K1333312
start: 1123
end: 1252
orientation: inline

BBa_I13458
type: SequenceAnnotation
persistent identity: annotation2386080
display id: annotation2386080
version: 1
top level: BBa_K1333312
location: https://synbiohub.org/public/igem/BBa_K1333312/annotation2386080/range2386080/1
component: BBa_I13458

https://synbiohub.org/public/igem/BBa_K1333312/annotation2386080/range2386080/1
type: Range
persistent identity: range2386080
display id: range2386080
version: 1
direction: direction/reverse
top level: BBa_K1333312
start: 1
end: 1114
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2386083
display id: annotation2386083
version: 1
top level: BBa_K1333312
location: https://synbiohub.org/public/igem/BBa_K1333312/annotation2386083/range2386083/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1333312/annotation2386083/range2386083/1
type: Range
persistent identity: range2386083
display id: range2386083
version: 1
direction: direction/reverse
top level: BBa_K1333312
start: 1261
end: 1272
orientation: inline

BBa_K1404007
type: ComponentDefinition
persistent identity: BBa K1404007
display id: BBa_K1404007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: p70-CsgA-His, His-tagged curli generator
creator: Juliette Paillet, Alexandre Duprey, Emiel van der Kouwe
created: 2014-09-23T11:00:00Z
modified: 2015-05-08T01:10:17Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1782_
m user id: 0
owner id: 21195
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: temp
mutable notes: temp
mutable provenance: temp
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: Beta sheet3
sequence annotation: RBS Paris 2009
sequence annotation: GCT into GCC
sequence annotation: BBa_K342000
sequence annotation: Beta sheet4
sequence annotation: CsgG signal
sequence annotation: Sec signal
sequence annotation: His tag
sequence annotation: CsgA-His1
sequence annotation: Beta sheet5
sequence annotation: Beta sheet2
sequence annotation: Linker
sequence annotation: Beta sheet 1
sequence: https://synbiohub.org/public/igem/BBa_K1404007_sequence/1

Beta sheet3
type: SequenceAnnotation
persistent identity: annotation2426225
display id: annotation2426225
version: 1
top level: BBa_K1404007
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1404007/annotation2426225/range2426225/1

https://synbiohub.org/public/igem/BBa_K1404007/annotation2426225/range2426225/1
type: Range
persistent identity: range2426225
display id: range2426225
version: 1
direction: direction/forward
top level: BBa_K1404007
start: 353
end: 421
orientation: inline

RBS Paris 2009
type: SequenceAnnotation
persistent identity: annotation2426220
display id: annotation2426220
version: 1
top level: BBa_K1404007
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1404007/annotation2426220/range2426220/1

https://synbiohub.org/public/igem/BBa_K1404007/annotation2426220/range2426220/1
type: Range
persistent identity: range2426220
display id: range2426220
version: 1
direction: direction/forward
top level: BBa_K1404007
start: 79
end: 91
orientation: inline

GCT into GCC
type: SequenceAnnotation
persistent identity: annotation2426227
display id: annotation2426227
version: 1
top level: BBa_K1404007
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K1404007/annotation2426227/range2426227/1

https://synbiohub.org/public/igem/BBa_K1404007/annotation2426227/range2426227/1
type: Range
persistent identity: range2426227
display id: range2426227
version: 1
direction: direction/forward
top level: BBa_K1404007
start: 461
end: 463
orientation: inline

BBa_K342000
type: SequenceAnnotation
persistent identity: annotation2426218
display id: annotation2426218
version: 1
top level: BBa_K1404007
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1404007/annotation2426218/range2426218/1

https://synbiohub.org/public/igem/BBa_K1404007/annotation2426218/range2426218/1
type: Range
persistent identity: range2426218
display id: range2426218
version: 1
direction: direction/forward
top level: BBa_K1404007
start: 1
end: 70
orientation: inline

Beta sheet4
type: SequenceAnnotation
persistent identity: annotation2426226
display id: annotation2426226
version: 1
top level: BBa_K1404007
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1404007/annotation2426226/range2426226/1

https://synbiohub.org/public/igem/BBa_K1404007/annotation2426226/range2426226/1
type: Range
persistent identity: range2426226
display id: range2426226
version: 1
direction: direction/forward
top level: BBa_K1404007
start: 422
end: 487
orientation: inline

CsgG signal
type: SequenceAnnotation
persistent identity: annotation2426222
display id: annotation2426222
version: 1
top level: BBa_K1404007
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1404007/annotation2426222/range2426222/1

https://synbiohub.org/public/igem/BBa_K1404007/annotation2426222/range2426222/1
type: Range
persistent identity: range2426222
display id: range2426222
version: 1
direction: direction/forward
top level: BBa_K1404007
start: 152
end: 217
orientation: inline

Sec signal
type: SequenceAnnotation
persistent identity: annotation2426221
display id: annotation2426221
version: 1
top level: BBa_K1404007
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1404007/annotation2426221/range2426221/1

https://synbiohub.org/public/igem/BBa_K1404007/annotation2426221/range2426221/1
type: Range
persistent identity: range2426221
display id: range2426221
version: 1
direction: direction/forward
top level: BBa_K1404007
start: 92
end: 151
orientation: inline

His tag
type: SequenceAnnotation
persistent identity: annotation2426230
display id: annotation2426230
version: 1
top level: BBa_K1404007
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1404007/annotation2426230/range2426230/1

https://synbiohub.org/public/igem/BBa_K1404007/annotation2426230/range2426230/1
type: Range
persistent identity: range2426230
display id: range2426230
version: 1
direction: direction/forward
top level: BBa_K1404007
start: 557
end: 574
orientation: inline

CsgA-His1
type: SequenceAnnotation
persistent identity: annotation2426219
display id: annotation2426219
version: 1
top level: BBa_K1404007
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1404007/annotation2426219/range2426219/1

https://synbiohub.org/public/igem/BBa_K1404007/annotation2426219/range2426219/1
type: Range
persistent identity: range2426219
display id: range2426219
version: 1
direction: direction/forward
top level: BBa_K1404007
start: 92
end: 577
orientation: inline

Beta sheet5
type: SequenceAnnotation
persistent identity: annotation2426228
display id: annotation2426228
version: 1
top level: BBa_K1404007
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1404007/annotation2426228/range2426228/1

https://synbiohub.org/public/igem/BBa_K1404007/annotation2426228/range2426228/1
type: Range
persistent identity: range2426228
display id: range2426228
version: 1
direction: direction/forward
top level: BBa_K1404007
start: 488
end: 544
orientation: inline

Beta sheet2
type: SequenceAnnotation
persistent identity: annotation2426224
display id: annotation2426224
version: 1
top level: BBa_K1404007
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1404007/annotation2426224/range2426224/1

https://synbiohub.org/public/igem/BBa_K1404007/annotation2426224/range2426224/1
type: Range
persistent identity: range2426224
display id: range2426224
version: 1
direction: direction/forward
top level: BBa_K1404007
start: 287
end: 352
orientation: inline

Linker
type: SequenceAnnotation
persistent identity: annotation2426229
display id: annotation2426229
version: 1
top level: BBa_K1404007
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1404007/annotation2426229/range2426229/1

https://synbiohub.org/public/igem/BBa_K1404007/annotation2426229/range2426229/1
type: Range
persistent identity: range2426229
display id: range2426229
version: 1
direction: direction/forward
top level: BBa_K1404007
start: 545
end: 556
orientation: inline

Beta sheet 1
type: SequenceAnnotation
persistent identity: annotation2426223
display id: annotation2426223
version: 1
top level: BBa_K1404007
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1404007/annotation2426223/range2426223/1

https://synbiohub.org/public/igem/BBa_K1404007/annotation2426223/range2426223/1
type: Range
persistent identity: range2426223
display id: range2426223
version: 1
direction: direction/forward
top level: BBa_K1404007
start: 218
end: 286
orientation: inline

BBa_K1404006
type: ComponentDefinition
persistent identity: BBa K1404006
display id: BBa_K1404006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: p70-CsgA, curli generator
creator: Juliette Paillet, Alexandre Duprey, Emiel van der Kouwe
created: 2014-09-23T11:00:00Z
modified: 2015-05-08T01:10:17Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1782_
m user id: 0
owner id: 21195
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: temp
mutable notes: temp
mutable provenance: temp
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: CsgG signal
sequence annotation: Beta sheet2
sequence annotation: Beta sheet4
sequence annotation: mutation
sequence annotation: CsgA-WT
sequence annotation: Beta sheet5
sequence annotation: Part:BBa_K342000
sequence annotation: Sec signal
sequence annotation: RBS Paris 2009
sequence annotation: Beta sheet3
sequence annotation: Beta sheet1
sequence: https://synbiohub.org/public/igem/BBa_K1404006_sequence/1

CsgG signal
type: SequenceAnnotation
persistent identity: annotation2425825
display id: annotation2425825
version: 1
top level: BBa_K1404006
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1404006/annotation2425825/range2425825/1

https://synbiohub.org/public/igem/BBa_K1404006/annotation2425825/range2425825/1
type: Range
persistent identity: range2425825
display id: range2425825
version: 1
direction: direction/forward
top level: BBa_K1404006
start: 152
end: 217
orientation: inline

Beta sheet2
type: SequenceAnnotation
persistent identity: annotation2425860
display id: annotation2425860
version: 1
top level: BBa_K1404006
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1404006/annotation2425860/range2425860/1

https://synbiohub.org/public/igem/BBa_K1404006/annotation2425860/range2425860/1
type: Range
persistent identity: range2425860
display id: range2425860
version: 1
direction: direction/forward
top level: BBa_K1404006
start: 287
end: 352
orientation: inline

Beta sheet4
type: SequenceAnnotation
persistent identity: annotation2425862
display id: annotation2425862
version: 1
top level: BBa_K1404006
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1404006/annotation2425862/range2425862/1

https://synbiohub.org/public/igem/BBa_K1404006/annotation2425862/range2425862/1
type: Range
persistent identity: range2425862
display id: range2425862
version: 1
direction: direction/forward
top level: BBa_K1404006
start: 422
end: 487
orientation: inline

mutation
type: SequenceAnnotation
persistent identity: annotation2425844
display id: annotation2425844
version: 1
top level: BBa_K1404006
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K1404006/annotation2425844/range2425844/1

https://synbiohub.org/public/igem/BBa_K1404006/annotation2425844/range2425844/1
type: Range
persistent identity: range2425844
display id: range2425844
version: 1
direction: direction/forward
top level: BBa_K1404006
start: 461
end: 463
orientation: inline

CsgA-WT
type: SequenceAnnotation
persistent identity: annotation2425824
display id: annotation2425824
version: 1
top level: BBa_K1404006
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1404006/annotation2425824/range2425824/1

https://synbiohub.org/public/igem/BBa_K1404006/annotation2425824/range2425824/1
type: Range
persistent identity: range2425824
display id: range2425824
version: 1
direction: direction/forward
top level: BBa_K1404006
start: 92
end: 547
orientation: inline

Beta sheet5
type: SequenceAnnotation
persistent identity: annotation2425863
display id: annotation2425863
version: 1
top level: BBa_K1404006
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1404006/annotation2425863/range2425863/1

https://synbiohub.org/public/igem/BBa_K1404006/annotation2425863/range2425863/1
type: Range
persistent identity: range2425863
display id: range2425863
version: 1
direction: direction/forward
top level: BBa_K1404006
start: 488
end: 544
orientation: inline

Part:BBa_K342000
type: SequenceAnnotation
persistent identity: annotation2425822
display id: annotation2425822
version: 1
top level: BBa_K1404006
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1404006/annotation2425822/range2425822/1

https://synbiohub.org/public/igem/BBa_K1404006/annotation2425822/range2425822/1
type: Range
persistent identity: range2425822
display id: range2425822
version: 1
direction: direction/forward
top level: BBa_K1404006
start: 1
end: 70
orientation: inline

Sec signal
type: SequenceAnnotation
persistent identity: annotation2425823
display id: annotation2425823
version: 1
top level: BBa_K1404006
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1404006/annotation2425823/range2425823/1

https://synbiohub.org/public/igem/BBa_K1404006/annotation2425823/range2425823/1
type: Range
persistent identity: range2425823
display id: range2425823
version: 1
direction: direction/forward
top level: BBa_K1404006
start: 92
end: 151
orientation: inline

RBS Paris 2009
type: SequenceAnnotation
persistent identity: annotation2425875
display id: annotation2425875
version: 1
top level: BBa_K1404006
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1404006/annotation2425875/range2425875/1

https://synbiohub.org/public/igem/BBa_K1404006/annotation2425875/range2425875/1
type: Range
persistent identity: range2425875
display id: range2425875
version: 1
direction: direction/forward
top level: BBa_K1404006
start: 79
end: 91
orientation: inline

Beta sheet3
type: SequenceAnnotation
persistent identity: annotation2425861
display id: annotation2425861
version: 1
top level: BBa_K1404006
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1404006/annotation2425861/range2425861/1

https://synbiohub.org/public/igem/BBa_K1404006/annotation2425861/range2425861/1
type: Range
persistent identity: range2425861
display id: range2425861
version: 1
direction: direction/forward
top level: BBa_K1404006
start: 353
end: 421
orientation: inline

Beta sheet1
type: SequenceAnnotation
persistent identity: annotation2425859
display id: annotation2425859
version: 1
top level: BBa_K1404006
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1404006/annotation2425859/range2425859/1

https://synbiohub.org/public/igem/BBa_K1404006/annotation2425859/range2425859/1
type: Range
persistent identity: range2425859
display id: range2425859
version: 1
direction: direction/forward
top level: BBa_K1404006
start: 218
end: 286
orientation: inline

BBa_K1666000
type: ComponentDefinition
persistent identity: BBa K1666000
display id: BBa_K1666000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LsrA of LuxS/AI-2 signaling pathway in Salmonalla
creator: Heming Wang
created: 2015-08-25T11:00:00Z
modified: 2015-08-30T07:46:12Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2084_
m user id: 28478
owner id: 28478
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: In a process called quorum sensing, bacteria communicate with one another using secreted chemical signaling molecules termed autoinducers. As the density of the bacterial population increases, so does the concentration of secreted autoinducer molecules. When the concentration of the autoinducer reaches a critical threshold, it diffuses back into the cell and activates or represses certain target genes. An autoinducer called AI-2 is generated by many species of Gram-negative and Gram-positive bacteria. In the LuxS/AI-2 signaling system of Salmonella enterica serovar Typhimurium, AI-2 response involves genes named lsr (LuxS regulated) that encode ATP binding cassette-type transporter. And LsrA is ATP-binding protein of the ABC transporter. In our project, we set this part under a nisA promoter for the final purpose of constructing an integrated AI-2 response pathway in our engineering bacteria.
mutable notes: The lsrA gene was isolated from the genome of Salmonella enterica subsp. enterica serovar Typhimurium str. LT2 using PCR.We added restriction enzyme sites when designing primers,and the assembly is done through the use of restriction sites (cutting and ligating).
mutable provenance: It comes from the genome of Salmonella enterica subsp. enterica serovar Typhimurium str. LT2(Gene ID: 1255601)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: LsrA
sequence: https://synbiohub.org/public/igem/BBa_K1666000_sequence/1

LsrA
type: SequenceAnnotation
persistent identity: annotation2439289
display id: annotation2439289
version: 1
top level: BBa_K1666000
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1666000/annotation2439289/range2439289/1

https://synbiohub.org/public/igem/BBa_K1666000/annotation2439289/range2439289/1
type: Range
persistent identity: range2439289
display id: range2439289
version: 1
direction: direction/forward
top level: BBa_K1666000
start: 1
end: 1536
orientation: inline

LipA-EAK16
type: ComponentDefinition
persistent identity: BBa K143034
display id: BBa_K143034
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LipA-EAK16-II Fusion Protein
creator: Qin Qi
created: 2008-09-17T11:00:00Z
modified: 2015-05-08T01:10:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _199_
m user id: 0
owner id: 3429
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: EAK16-II is a sixteen amino acid peptide that self-assembles to form β-sheet structures in an aqueous medium. The alternating positive and negative charges (--++--++) are responsible for creating an electrostatic attraction between adjacent peptides 1, triggering self-assembly when the EAK16-II peptides are exposed to physiological media or salt solution. When examined under SEM, a well-ordered nanofibre structure is formed by the association of the EAK16-II peptides and these nanofibres can futher aggregate to form a membranous 3D scaffold. LipA is a signal peptide from the B.subtilis genome. In general, signal peptides are responsible for directing preproteins (secretory proteins with a signal peptide region attached)through an appropriate secretory pathway2. LipA has been successfully used in the secretion of heterologous proteins such as cutinase by ''B. subtilis''.
mutable notes: BioBrick standard was applied to LipA-EAK16II Fusion Protein.
mutable provenance: EAK16-II was identified as a region in zuotin, a Z-DNA binding protein from the yeast genome. LipA originated from the B. subtilis genome3. Both components were produced as a fusion protein by GeneArt.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: stop
sequence annotation: EAK16-II
sequence annotation: start
sequence annotation: LipA
sequence: https://synbiohub.org/public/igem/BBa_K143034_sequence/1

stop
type: SequenceAnnotation
persistent identity: annotation1992705
display id: annotation1992705
version: 1
top level: LipA-EAK16
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K143034/annotation1992705/range1992705/1

https://synbiohub.org/public/igem/BBa_K143034/annotation1992705/range1992705/1
type: Range
persistent identity: range1992705
display id: range1992705
version: 1
direction: direction/forward
top level: LipA-EAK16
start: 151
end: 153
orientation: inline

EAK16-II
type: SequenceAnnotation
persistent identity: annotation1992703
display id: annotation1992703
version: 1
top level: LipA-EAK16
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K143034/annotation1992703/range1992703/1

https://synbiohub.org/public/igem/BBa_K143034/annotation1992703/range1992703/1
type: Range
persistent identity: range1992703
display id: range1992703
version: 1
direction: direction/forward
top level: LipA-EAK16
start: 103
end: 150
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1992704
display id: annotation1992704
version: 1
top level: LipA-EAK16
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K143034/annotation1992704/range1992704/1

https://synbiohub.org/public/igem/BBa_K143034/annotation1992704/range1992704/1
type: Range
persistent identity: range1992704
display id: range1992704
version: 1
direction: direction/forward
top level: LipA-EAK16
start: 154
end: 156
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation1992706
display id: annotation1992706
version: 1
top level: LipA-EAK16
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K143034/annotation1992706/range1992706/1

https://synbiohub.org/public/igem/BBa_K143034/annotation1992706/range1992706/1
type: Range
persistent identity: range1992706
display id: range1992706
version: 1
direction: direction/forward
top level: LipA-EAK16
start: 1
end: 3
orientation: inline

LipA
type: SequenceAnnotation
persistent identity: annotation1976427
display id: annotation1976427
version: 1
top level: LipA-EAK16
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K143034/annotation1976427/range1976427/1

https://synbiohub.org/public/igem/BBa_K143034/annotation1976427/range1976427/1
type: Range
persistent identity: range1976427
display id: range1976427
version: 1
direction: direction/forward
top level: LipA-EAK16
start: 1
end: 102
orientation: inline